LOCUS       DACDHL010000001        99849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDHL010000001 DACDHL010000000
VERSION     DACDHL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.001"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            598..1287
                     /locus_tag="IV228_000001"
     CDS             598..1287
                     /locus_tag="IV228_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8715957.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="IV228_000002"
     CDS             1274..2482
                     /locus_tag="IV228_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8715958.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="IV228_000003"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="IV228_000003"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8715959.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IV228_000004"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IV228_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8715960.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="IV228_000005"
     CDS             complement(6585..7280)
                     /locus_tag="IV228_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8715961.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IV228_000006"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IV228_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8715962.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="IV228_000007"
     CDS             complement(8479..8670)
                     /locus_tag="IV228_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8715963.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="IV228_000008"
     CDS             complement(8691..9581)
                     /locus_tag="IV228_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8715964.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="IV228_000009"
     CDS             complement(9568..10335)
                     /locus_tag="IV228_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8715965.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IV228_000010"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IV228_000010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8715966.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IV228_000011"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IV228_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8715967.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IV228_000012"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IV228_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8715968.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14951..15289
                     /locus_tag="IV228_000013"
     CDS             14951..15289
                     /locus_tag="IV228_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8715969.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="IV228_000014"
     CDS             complement(15457..16215)
                     /locus_tag="IV228_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8715970.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="IV228_000015"
     CDS             complement(16233..17051)
                     /locus_tag="IV228_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8715971.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IV228_000016"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IV228_000016"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8715972.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IV228_000017"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IV228_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8715973.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18180..19514
                     /gene="dnaA"
                     /locus_tag="IV228_000018"
     CDS             18180..19514
                     /gene="dnaA"
                     /locus_tag="IV228_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8715974.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19712..20842
                     /gene="dnaN"
                     /locus_tag="IV228_000019"
     CDS             19712..20842
                     /gene="dnaN"
                     /locus_tag="IV228_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8715975.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21071..21313
                     /gene="yaaA"
                     /locus_tag="IV228_000020"
     CDS             21071..21313
                     /gene="yaaA"
                     /locus_tag="IV228_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8715976.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21300..22424
                     /gene="recF"
                     /locus_tag="IV228_000021"
     CDS             21300..22424
                     /gene="recF"
                     /locus_tag="IV228_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8715977.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22421..24367
                     /gene="gyrB"
                     /locus_tag="IV228_000022"
     CDS             22421..24367
                     /gene="gyrB"
                     /locus_tag="IV228_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8715978.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24387..26858
                     /gene="gyrA"
                     /locus_tag="IV228_000023"
     CDS             24387..26858
                     /gene="gyrA"
                     /locus_tag="IV228_000023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8715979.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27094..27393
                     /locus_tag="IV228_000024"
     CDS             27094..27393
                     /locus_tag="IV228_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8715980.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27443..27955
                     /gene="ssb"
                     /locus_tag="IV228_000025"
     CDS             27443..27955
                     /gene="ssb"
                     /locus_tag="IV228_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8715981.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27981..28217
                     /gene="rpsR"
                     /locus_tag="IV228_000026"
     CDS             27981..28217
                     /gene="rpsR"
                     /locus_tag="IV228_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8715982.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28360..30333
                     /locus_tag="IV228_000027"
     CDS             28360..30333
                     /locus_tag="IV228_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8715983.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30339..30791
                     /gene="rplI"
                     /locus_tag="IV228_000028"
     CDS             30339..30791
                     /gene="rplI"
                     /locus_tag="IV228_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8715984.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31065..32432
                     /gene="dnaB"
                     /locus_tag="IV228_000029"
     CDS             31065..32432
                     /gene="dnaB"
                     /locus_tag="IV228_000029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8715985.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33212..34504
                     /locus_tag="IV228_000030"
     CDS             33212..34504
                     /locus_tag="IV228_000030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8715986.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35291..36154
                     /locus_tag="IV228_000031"
     CDS             35291..36154
                     /locus_tag="IV228_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8715987.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36283..37905)
                     /locus_tag="IV228_000032"
     CDS             complement(36283..37905)
                     /locus_tag="IV228_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8715988.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38154..38675
                     /locus_tag="IV228_000033"
     CDS             38154..38675
                     /locus_tag="IV228_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8715989.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38861..40591)
                     /gene="spxB"
                     /locus_tag="IV228_000034"
     CDS             complement(38861..40591)
                     /gene="spxB"
                     /locus_tag="IV228_000034"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8715990.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40756..41574
                     /locus_tag="IV228_000035"
     CDS             40756..41574
                     /locus_tag="IV228_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8715991.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41677..41874)
                     /locus_tag="IV228_000036"
     CDS             complement(41677..41874)
                     /locus_tag="IV228_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8715992.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42043..42621
                     /locus_tag="IV228_000037"
     CDS             42043..42621
                     /locus_tag="IV228_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8715993.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43666..44169)
                     /locus_tag="IV228_000038"
     CDS             complement(43666..44169)
                     /locus_tag="IV228_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8715994.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44528..45553
                     /locus_tag="IV228_000039"
     CDS             44528..45553
                     /locus_tag="IV228_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8715995.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45876..46754
                     /locus_tag="IV228_000040"
     CDS             45876..46754
                     /locus_tag="IV228_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8715996.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47230..47346
                     /locus_tag="IV228_000041"
     CDS             47230..47346
                     /locus_tag="IV228_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8715997.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48274..48612
                     /locus_tag="IV228_000042"
     CDS             48274..48612
                     /locus_tag="IV228_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8715998.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48706..48936
                     /locus_tag="IV228_000043"
     CDS             48706..48936
                     /locus_tag="IV228_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8715999.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49336..49707
                     /locus_tag="IV228_000044"
     CDS             49336..49707
                     /locus_tag="IV228_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8716000.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49981..50673)
                     /locus_tag="IV228_000045"
     CDS             complement(49981..50673)
                     /locus_tag="IV228_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8716001.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50676..51461)
                     /locus_tag="IV228_000046"
     CDS             complement(50676..51461)
                     /locus_tag="IV228_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8716002.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51698..53098
                     /gene="lacG"
                     /locus_tag="IV228_000047"
     CDS             51698..53098
                     /gene="lacG"
                     /locus_tag="IV228_000047"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8716003.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53123..53440
                     /locus_tag="IV228_000048"
     CDS             53123..53440
                     /locus_tag="IV228_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8716004.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53453..55126
                     /locus_tag="IV228_000049"
     CDS             53453..55126
                     /locus_tag="IV228_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8716005.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55140..56771
                     /locus_tag="IV228_000050"
     CDS             55140..56771
                     /locus_tag="IV228_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8716006.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57262..58827)
                     /gene="guaA"
                     /locus_tag="IV228_000051"
     CDS             complement(57262..58827)
                     /gene="guaA"
                     /locus_tag="IV228_000051"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8716007.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58886..59809)
                     /locus_tag="IV228_000052"
     CDS             complement(58886..59809)
                     /locus_tag="IV228_000052"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8716008.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            60005..60610
                     /locus_tag="IV228_000053"
     CDS             60005..60610
                     /locus_tag="IV228_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8716009.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60918..61934
                     /locus_tag="IV228_000054"
     CDS             60918..61934
                     /locus_tag="IV228_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8716010.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62075..63376
                     /locus_tag="IV228_000055"
     CDS             62075..63376
                     /locus_tag="IV228_000055"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8716011.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63422..64084
                     /gene="deoC"
                     /locus_tag="IV228_000056"
     CDS             63422..64084
                     /gene="deoC"
                     /locus_tag="IV228_000056"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8716012.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64105..64503
                     /locus_tag="IV228_000057"
     CDS             64105..64503
                     /locus_tag="IV228_000057"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8716013.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64569..65651
                     /locus_tag="IV228_000058"
     CDS             64569..65651
                     /locus_tag="IV228_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8716014.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65863..67425
                     /locus_tag="IV228_000059"
     CDS             65863..67425
                     /locus_tag="IV228_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716015.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67418..68551
                     /locus_tag="IV228_000060"
     CDS             67418..68551
                     /locus_tag="IV228_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716016.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68551..69507
                     /locus_tag="IV228_000061"
     CDS             68551..69507
                     /locus_tag="IV228_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716017.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69606..70772
                     /gene="deoB"
                     /locus_tag="IV228_000062"
     CDS             69606..70772
                     /gene="deoB"
                     /locus_tag="IV228_000062"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8716018.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70796..71614
                     /locus_tag="IV228_000063"
     CDS             70796..71614
                     /locus_tag="IV228_000063"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8716019.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71631..72338
                     /gene="deoD"
                     /locus_tag="IV228_000064"
     CDS             71631..72338
                     /gene="deoD"
                     /locus_tag="IV228_000064"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8716020.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72636..73322)
                     /gene="gpmA"
                     /locus_tag="IV228_000065"
     CDS             complement(72636..73322)
                     /gene="gpmA"
                     /locus_tag="IV228_000065"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8716021.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73494..74075)
                     /locus_tag="IV228_000066"
     CDS             complement(73494..74075)
                     /locus_tag="IV228_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8716022.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74208..74891)
                     /gene="rpiA"
                     /locus_tag="IV228_000067"
     CDS             complement(74208..74891)
                     /gene="rpiA"
                     /locus_tag="IV228_000067"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8716023.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75210..75623
                     /gene="rpsL"
                     /locus_tag="IV228_000068"
     CDS             75210..75623
                     /gene="rpsL"
                     /locus_tag="IV228_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8716024.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75707..76177
                     /gene="rpsG"
                     /locus_tag="IV228_000069"
     CDS             75707..76177
                     /gene="rpsG"
                     /locus_tag="IV228_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8716025.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76271..78358
                     /gene="fusA"
                     /locus_tag="IV228_000070"
     CDS             76271..78358
                     /gene="fusA"
                     /locus_tag="IV228_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8716026.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78530..79717
                     /gene="tuf"
                     /locus_tag="IV228_000071"
     CDS             78530..79717
                     /gene="tuf"
                     /locus_tag="IV228_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8716027.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80124..80432
                     /gene="rpsJ"
                     /locus_tag="IV228_000072"
     CDS             80124..80432
                     /gene="rpsJ"
                     /locus_tag="IV228_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8716028.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80463..81092
                     /gene="rplC"
                     /locus_tag="IV228_000073"
     CDS             80463..81092
                     /gene="rplC"
                     /locus_tag="IV228_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8716029.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81120..81743
                     /gene="rplD"
                     /locus_tag="IV228_000074"
     CDS             81120..81743
                     /gene="rplD"
                     /locus_tag="IV228_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8716030.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81743..82033
                     /gene="rplW"
                     /locus_tag="IV228_000075"
     CDS             81743..82033
                     /gene="rplW"
                     /locus_tag="IV228_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8716031.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82072..82905
                     /gene="rplB"
                     /locus_tag="IV228_000076"
     CDS             82072..82905
                     /gene="rplB"
                     /locus_tag="IV228_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8716032.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            82948..83226
                     /gene="rpsS"
                     /locus_tag="IV228_000077"
     CDS             82948..83226
                     /gene="rpsS"
                     /locus_tag="IV228_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8716033.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83249..83596
                     /gene="rplV"
                     /locus_tag="IV228_000078"
     CDS             83249..83596
                     /gene="rplV"
                     /locus_tag="IV228_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8716034.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83610..84266
                     /gene="rpsC"
                     /locus_tag="IV228_000079"
     CDS             83610..84266
                     /gene="rpsC"
                     /locus_tag="IV228_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8716035.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84269..84703
                     /gene="rplP"
                     /locus_tag="IV228_000080"
     CDS             84269..84703
                     /gene="rplP"
                     /locus_tag="IV228_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8716036.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84693..84881
                     /gene="rpmC"
                     /locus_tag="IV228_000081"
     CDS             84693..84881
                     /gene="rpmC"
                     /locus_tag="IV228_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8716037.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            84906..85172
                     /gene="rpsQ"
                     /locus_tag="IV228_000082"
     CDS             84906..85172
                     /gene="rpsQ"
                     /locus_tag="IV228_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8716038.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85230..85598
                     /gene="rplN"
                     /locus_tag="IV228_000083"
     CDS             85230..85598
                     /gene="rplN"
                     /locus_tag="IV228_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8716039.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85635..85943
                     /gene="rplX"
                     /locus_tag="IV228_000084"
     CDS             85635..85943
                     /gene="rplX"
                     /locus_tag="IV228_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8716040.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            85971..86510
                     /gene="rplE"
                     /locus_tag="IV228_000085"
     CDS             85971..86510
                     /gene="rplE"
                     /locus_tag="IV228_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8716041.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86529..86714
                     /locus_tag="IV228_000086"
     CDS             86529..86714
                     /locus_tag="IV228_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8716042.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86750..87148
                     /gene="rpsH"
                     /locus_tag="IV228_000087"
     CDS             86750..87148
                     /gene="rpsH"
                     /locus_tag="IV228_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8716043.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87180..87716
                     /gene="rplF"
                     /locus_tag="IV228_000088"
     CDS             87180..87716
                     /gene="rplF"
                     /locus_tag="IV228_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8716044.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            87877..88233
                     /gene="rplR"
                     /locus_tag="IV228_000089"
     CDS             87877..88233
                     /gene="rplR"
                     /locus_tag="IV228_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8716045.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88254..88754
                     /gene="rpsE"
                     /locus_tag="IV228_000090"
     CDS             88254..88754
                     /gene="rpsE"
                     /locus_tag="IV228_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8716046.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            88769..88948
                     /gene="rpmD"
                     /locus_tag="IV228_000091"
     CDS             88769..88948
                     /gene="rpmD"
                     /locus_tag="IV228_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8716047.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            88992..89432
                     /gene="rplO"
                     /locus_tag="IV228_000092"
     CDS             88992..89432
                     /gene="rplO"
                     /locus_tag="IV228_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8716048.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89432..90727
                     /gene="secY"
                     /locus_tag="IV228_000093"
     CDS             89432..90727
                     /gene="secY"
                     /locus_tag="IV228_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8716049.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            90789..91436
                     /locus_tag="IV228_000094"
     CDS             90789..91436
                     /locus_tag="IV228_000094"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8716050.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91627..91845
                     /gene="infA"
                     /locus_tag="IV228_000095"
     CDS             91627..91845
                     /gene="infA"
                     /locus_tag="IV228_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8716051.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            91878..91994
                     /gene="rpmJ"
                     /locus_tag="IV228_000096"
     CDS             91878..91994
                     /gene="rpmJ"
                     /locus_tag="IV228_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8716052.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92012..92377
                     /gene="rpsM"
                     /locus_tag="IV228_000097"
     CDS             92012..92377
                     /gene="rpsM"
                     /locus_tag="IV228_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8716053.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92405..92794
                     /gene="rpsK"
                     /locus_tag="IV228_000098"
     CDS             92405..92794
                     /gene="rpsK"
                     /locus_tag="IV228_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8716054.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            92874..93812
                     /locus_tag="IV228_000099"
     CDS             92874..93812
                     /locus_tag="IV228_000099"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8716055.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            93840..94220
                     /gene="rplQ"
                     /locus_tag="IV228_000100"
     CDS             93840..94220
                     /gene="rplQ"
                     /locus_tag="IV228_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8716056.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94492..95331
                     /locus_tag="IV228_000101"
     CDS             94492..95331
                     /locus_tag="IV228_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8716057.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95307..96176
                     /locus_tag="IV228_000102"
     CDS             95307..96176
                     /locus_tag="IV228_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8716058.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96166..96963
                     /locus_tag="IV228_000103"
     CDS             96166..96963
                     /locus_tag="IV228_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8716059.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            97166..97912
                     /gene="truA"
                     /locus_tag="IV228_000104"
     CDS             97166..97912
                     /gene="truA"
                     /locus_tag="IV228_000104"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8716060.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            98577..98814
                     /locus_tag="IV228_000105"
                     /pseudo
     CDS             98577..98814
                     /locus_tag="IV228_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(98958..99734)
                     /locus_tag="IV228_000106"
     CDS             complement(98958..99734)
                     /locus_tag="IV228_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8716061.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT        31213 a        18391 c        20627 g        29618 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgcggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggag tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaatgg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg tgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tatatttttt atccacagat gcaagaggaa atctattgat ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa cttttttttg acaactgtgt ttttctcttt tttctttttg
    18061 tcgattgtgt ggataattgt aattaaaaaa tttttttctt cggtttctgt ggaaaactat
    18121 tgaaaaaaat gctaaattag tactagttat tcacttttta atgaaggagg aggccttata
    18181 tggtatccct cgatgcttta tggaatgaat taaaagcaac ataccaaaag gatttatcgc
    18241 ctgctagcta taatacatgg atcgaaacag cgaaccctcg aacccttgat caaaaccagc
    18301 tcgttgttga ggttcctagc aagattcata aagaatattg ggaaaaaaac ctggcgacaa
    18361 aaatcgttga agtcggttat atgttatcag gaaatgagat tatccctcgc tttattactg
    18421 gcgaagaagc agagcaagaa gaagttatag aagaaaaaaa tccaaaagtt gtggcaccta
    18481 gtccgctaaa aaaagccatg ctgaacccta aatatacctt tgatacgttt gtcattggta
    18541 aaggaaacca gatggctcat gcagcagcgc tcgttgtagc agaagatcca ggttctattt
    18601 ataacccgct cttcttttat ggaggcgtgg gacttggtaa gactcacttg atgcatgcaa
    18661 ttggtcatca aatgctgcaa agtcagccta atgccaaagt aaaatatgta agcagtgaaa
    18721 cttttgctaa tgatttcatc aattcgatcc aaaacaaaac agcagaagag ttccgtcaag
    18781 aatatcgaaa tgttgattta ctgctagtgg atgacattca attttttgca gaaaaagaag
    18841 ctacgcaaga agagtttttc catactttca atgccttgta caatgaaggg aaacaaatcg
    18901 tactaaccag tgatcggttg ccaaacgaga ttccgaaact gcaagaacgc ctcgtttctc
    18961 gctttgcatg ggggctgtct gttgatatta caccgccgga tcttgaaaca agaacagcga
    19021 ttttacgtaa aaaagcggcg gctgaacgtc tagagatccc agatgacaca ctaagctaca
    19081 tcgcaggaca aattgattct aacattcgtg aattagaagg tgcgcttgtt cgagtgcaag
    19141 cttttgctgc gatgaatagt gaagatattt cgactagtct agctgcagac gcactgaaaa
    19201 cgttaaaatc aggaaaagga catcctcaat tgtcaatcct gcagatccaa gaagaagtgg
    19261 caaagtatta tcacatccaa ttgaaagata tcaaaggaaa gaaacgcgtc aaatctattg
    19321 ttgttcctag acaaatcgct atgtatttgg cacgtgagct cacagataat tctttaccga
    19381 aaatcggagc tgaattcggt ggcaaggatc atacaacggt tatccatgct catgaaaaaa
    19441 tccagcagct gatggattct agtgtcagta tgcaaaatga agtatcagaa atcaaaaact
    19501 ttttactaaa ttaatttgtc gattctatca actgaaaata gcatgtggat aaaagaaaaa
    19561 aacgttcaaa agttatgcac actttttcca cagctgtttt ttcgtttctc tcttacgttt
    19621 tttgagtttt ccacagaatc aacatgccct attactttta ttattctttt taactaataa
    19681 taaataaaca aacattcaaa ggagatacat catgaaagtt actttaaacc gagctagctt
    19741 tatgcaggaa ttgcaaactg ttcaacgagc tatttcaagc aaaaccacga tccctatttt
    19801 gacaggtgta aaaatcacac tgacacaaga aggtttgact ttgacgggga gcaacgctga
    19861 tatatcaatt gaaacttttt tgtctgttga aaacgaaaaa gcaaatatgc aaatcgaatc
    19921 tactggttcc attgttttac aagcacgttt ctttagcgaa atcattcgga gacttcctga
    19981 agaaacattt actttagaag ttttagaaaa taaacaagta gcgattactt ctggaaaagc
    20041 gaattttatc gtaaatggat tagatgcaga taactatcct catcttcctg ttgtcgaaag
    20101 ccataaccag atgaaattac ctgtacacgt attgactaaa ctaatcaacg aaacagtttt
    20161 tgctgtctca caacatgaga gtcgtccaat cttgacaggt gtgcatttca ttttatctga
    20221 taattcttta ttggctgtag ctactgattc tcaccgtcta agtcaacgcg tgattccagt
    20281 agaacaagcg gctgatcatt ttgatattgt tattcctgga aaaagtttga tcgaattatc
    20341 tcgctcatta acaaatgaag aagaaatcgt cgaaatcagc attatggaaa accaagtgtt
    20401 gttcaaaaca gaaacgatgt atttctattc ccgtttgcta gaaggaaact atcctgatac
    20461 caatcgtttg attccttcaa gttttaatac agaggttgaa ttttctgttc ctagcttttt
    20521 agcggcgatc gaacgggctt ctttactttc tcatgaaggc cgtaacaaca tcgttcgttt
    20581 atctattcgt ccagatgcgg ttgttttata cggaaattcg ccagaaatcg ggaaagtaga
    20641 agaaagcttg agttatacag caagcagtgg cgatccatta gatatttctt tcaatccgga
    20701 ttatatgaaa gcagcgttgc gtgcatttgg agatatgagc atcaaagtga aatttatctc
    20761 tgctattcgt ccatttacat tggaaccaac ggaagatggc gttcaattta ttcagctgat
    20821 tactcctgta cgtacaaatt aacaatgatt ttcattgttc ttgaaaattt aattgaaaag
    20881 aaatagaaaa aataaaattt taaacagaag gccctaaaat ctcttttaag gctttctgtt
    20941 tatttttgtt taaatcacct tttttagatt aaatcgtctt atatggaaaa atatatggat
    21001 aaatttgttt tattgcgaaa aaaaaggtat aatagagtat acttttaata atgaaaatga
    21061 gggatgggca ttgaaacaac aagtcattct aaatacggaa ttcatgacgt tgggccaaat
    21121 gttgaaagaa gtcacagtta tcggtagtgg cggacaagca aaatggtatt tggcagaaaa
    21181 cactgtgtta gtcgatggcg aaccagaaaa tcgtcgtgga cgcaaacttt atcccggcat
    21241 gatggttgaa gttccagaag taggaacttt ttttatggta aaaaaaggaa acgacgataa
    21301 tgaggctgaa taagctgtat ttaaaaaatt atcgaaatta cgaagagctg aacatcgaat
    21361 tttccaagaa cctcgtcatc tttttaggag aaaatgcgca aggaaaaacg aatcttttgg
    21421 aaagtatcta cgtattagct atgacgcgaa gtcatcggac aagtagtgag caggaattga
    21481 ttggctggac tgacgatcag gcgatgattc aaggagagat cacaaaaggt tcatccactc
    21541 ttccattaga aatcttatta tctaaaaaag gccgaaaaac caagatcaac catatcgaac
    21601 aaaaaaagtt aagtacttac gtgggacaaa tgaatgtcat tttatttgca ccagaagatc
    21661 tttctttagt aaaaggcagc ccgcagatca ggcggaagtt tttagatatg gaattaggcc
    21721 agatcaatcc aatctatctt tatgatcttg tccaatacca agcaattttg aaacaaagaa
    21781 accagtattt aaagcaattg aatgaaaaaa aacaaactga tttgctttat ttggatattt
    21841 tgtccgaaca actagcagcg tttggcagca aagtattgaa agcaagagca cagttcgtca
    21901 agcgattgga gttttgggct aattctttgc atcagcaaat cacccatcaa aaagagcagc
    21961 tggaaatcga gtatctcacc gcagtcgatt ctttagaaac acatacgcaa gagcaaatcc
    22021 aagaacaatt tctcgcactt ttgaaccaaa ataaaaagaa agaccttttt cggggaacaa
    22081 cgacagttgg tcctcatcgt gacgatcttt ccttttttat taatcagaaa aatgtgcaga
    22141 cttatggatc gcaaggacag caaagaacaa ctgcactaag tgtcaaatta gcagaaatcg
    22201 atttgattaa agaagagacg ggggaatatc caatcctttt gttagatgat gtgatgagcg
    22261 agctggatga tagtcgtcaa ctccatctgc tagaaacaat cgaaggaaaa gttcaaacgt
    22321 tcttaacaac aacgacactt gaccatgtaa aaaataaaat gactgttgaa cctgagattt
    22381 tttatgttca acaagggaaa ctaaaaggag aagaaactga atgacagaag aaagaagttt
    22441 agtagaacgc gctaaagagt atgatgccag tcagattcaa gtacttgaag gacttgaagc
    22501 cgttcgtaaa cgacctggca tgtatatcgg ttcgactagt tctgagggtc tgcatcacct
    22561 tgtttgggaa attgtcgata attcgatcga tgaagtatta gcaggttttg caacgaaaat
    22621 ccatgtgatt attgaaaaag acaacagtat caccgttatt gacgacggtc gggggatacc
    22681 tgtagatatc caggcaaaaa ctggacgtcc tgcagttgaa acggtcttta ctgttttaca
    22741 tgctggagga aaattcggcg gtggtgggta caaagtttct ggtggacttc acggagtagg
    22801 atcttctgtt gttaacgcgc tttctacaca gcttgatgtc aaagtataca aagaaggaaa
    22861 tgtgtactat caagaatatc gtcgtggagc tgtagtggac gacttaaaga ttatcgaaca
    22921 aacggatcgc catggaacaa cggttcactt tacgccagac cctgaaattt ttacagagac
    22981 aactgagttt gatttttcaa aattggctac ccggatccgt gaacttgctt tcttgaaccg
    23041 cgggatgcgt atatcgattg aagacaaacg agaagaagaa ccagtcatca atgaatacca
    23101 ttatgacggc gggattaaaa gttatgtcga atacttgaat gcaaacaaaa ccgtgatttt
    23161 tccagaacct gtatatttgg aaggtgaaca gcaagatatc gcagttgaag tctctatgca
    23221 atatacagac ggctaccatt ccaatatcat gagttttgcc aacaatatcc atacgtatga
    23281 aggcggaaca catgaatcag gattcaagac tgctttaact cgtgtgataa atgattatgc
    23341 ccgaaaacaa aagctgatga aagaaaatga cgacaacttg accggtgaag acgtgcgaga
    23401 aggattgaca gctgttattt ctatcaagca tcctgaccct caattcgaag gacaaacaaa
    23461 aacaaaattg ggcaattccg aagtacggac agtcactgat cggttattta gcgaacattt
    23521 catgaaattc ttgctggaaa acccaagtgt gggccgtcaa attgttgaaa aaggattact
    23581 agcttccaga gctcgtctag cagcgaaacg tgcgcgtgaa gtaacgagac gtaaaggtgc
    23641 tttagagatt agtaatctgc caggaaaatt agcggactgc tctagcaacg atcctgaaaa
    23701 atgcgaattg ttcatcgtcg aaggagattc ggccggcgga tcagctaagc aaggtcgtaa
    23761 tcgagagttt caagccatct tgcctattcg tgggaaaatc ttgaatgttg aaaaagcaag
    23821 catggacaag atcttggcaa atgaagagat ccggtcttta tttactgcta tgggtacagg
    23881 gtttggagaa gattttgatg tatcgaaagc tcgctaccac aaattagtta tcatgactga
    23941 tgccgatgtc gacggcgcgc atatccgtac attgctgctg actctttttt atcgttacat
    24001 gcgtcctatt gttgaagctg gatacgtcta tattgctcag ccacctttat acggggtaaa
    24061 acaagggaag aatattacgt atgtacaacc aggaaaaaat gcagaagaag agttgaaaca
    24121 agtcgttgct tcacttcctg cctcaccaaa accaagcgtt cagcgttata aaggacttgg
    24181 agaaatggat gatcatcaac tatgggaaac aactatggac ccaagcaatc ggatgatggc
    24241 ccgcgtcagt gtggacgatg ctattgcagc agatcagata tttgaaatgt tgatggggga
    24301 tcgagtagaa ccccgtcgtg cattcatcga ggaaaatgca cactatgtga agaacttaga
    24361 tatttaatca tgaaggagga aaaagaatga gtgaagaaat cagagaaaac atccatgatg
    24421 tcaatctaac cagcgaaatg aaagactcct tcatcgatta tgcgatgagt gttattgtcg
    24481 cacgtgcact tcctgatgtc agagatggtc tgaaacccgt tcatcgccgt attttatacg
    24541 gcatgaacga attaggtgta acaccggata aagcccataa aaaatctgcc agaatcgtcg
    24601 gagatgtcat gggtaaatac catcctcatg gagactatgc gatctatgag tcaatggtac
    24661 ggatggcaca gccattcagc taccgttata tgctagtgga tggtcatgga aactttggtt
    24721 ctgttgacgg tgacggtgcc gccgctatgc gttataccga agcacggatg agcaaaatcg
    24781 ctacagaaat gctgcgtgat atcaataaga acactgttga tttccaaagc aactatgatg
    24841 atacagaaaa agaaccagtt gtactacctg ctcgcttccc gaatctttta gtgaacggaa
    24901 caacggggat cgccgtaggt atggccacga atatcccacc tcataacttg tctgaggtag
    24961 tagcggctat cgatttgctg atggataatc ctgatgtgac gaccaatgaa ttgatggaag
    25021 tattacctgg accagatttt ccaacaggtg gtttggttat gggaaaatca ggaatccgaa
    25081 gagcgtatga aacaggtaag ggttctatta ccgtacgtgc aaaagttgag attactgaga
    25141 tgccaaatgg taaagaacgg atcatcgtga cagaattgcc ttatatggtc aataaagcaa
    25201 aattgatcga acggatttct gaactgcaca gagacaagcg tatcgaaggg atcacagatt
    25261 tgcgggacga atcttcccgt gaaggtatgc ggatcgtcat tgacatccgt cgtgacgcca
    25321 gcgcatctgt tatcttgaat aacttatata aattgacatc cttgcaaaat tcatttggat
    25381 tcaatatgtt ggcgatcgaa aaaggtgtgc cgaaagtttt gagcttgaaa caaattcttg
    25441 aaaactatat cgaacatcag cgcgaagtca tcacaagacg gacagtcttt gaaaaagaaa
    25501 aagcagaagc aagggcacac attttagaag gtttgcgtat tgctttggat catatcgatg
    25561 aaatcattgc gattatccgc gggtctaaat cagatgacga agcaaaagcg acgatgatcg
    25621 aacggttcga tttatccgat cgccagtctc aagctatttt agatatgcgt ttacgccgat
    25681 tgaccggttt ggaacgggaa aaaatcgaaa atgaatacca agaactctta aaactgattg
    25741 aagatttaac tgacattctg gcaagaccag aacgtgttac agaaatcatc aagaatgaat
    25801 tagcagactt gaatcaacga tttggtgata aacgacgcac agaattacta gtcggtgagg
    25861 tcttaagtct ggaagatgaa gatttaatcg aagaagaaga aatcgttatt acattgacaa
    25921 acaatggtta catcaaacgt ttggcaaata atgaattcag agctcaacga aggggtggac
    25981 gtggtgtcca aggaatgggt atccacaatg acgacttcgt caaaaatctg gtttcttgtt
    26041 ctacccatga tacattgtta ttctttacga ataacggaaa agtgtatcgg gcaaaaggat
    26101 acgaaattcc agaatacggg cgcacagcca aaggaatacc aattatcaat ttactaggta
    26161 tcgattcgag tgaaagcatc caggcaatca ttgcggtgac gggagaagca gaagaaggcc
    26221 actatctctt ctttacgacg cgtcaaggta cagtaaaacg tacttctgtc actgcttttg
    26281 ctaacatccg aagtaatggt ctgatcgcaa ttggtttgaa agaagacgat gagctcgtca
    26341 atgtgctact gacagatggt caatctaata tcattattgg aacacataac ggctactctg
    26401 tcactttcaa agaagaagca gtccgcgata tgggacgtac agcttccggg gttagaggaa
    26461 tacgcctgcg tgaggaagat tatgtcgttg gggcagcctt aatgaaggaa gatcaagaag
    26521 tattagtgat cacagaaaaa ggttacggaa aacggacaaa agtatcggaa tatccagtga
    26581 aaggccgcgg aggtaaaggg atcaaaacgg cgaacatcac tgaaaaaaat ggtccactag
    26641 ctggattgac cactgtttct ggagatgaag acatcatgct gattacagat aaaggtgtca
    26701 ttatccgttt caacgtatcg actgtttctc aaactggacg ttcaacatta ggcgtacgtt
    26761 tgatgaagat ggaagctgat acgaaagttg tcacgatggc agcagtcgaa ccagaagctg
    26821 cagaacaaac agttgaagaa caaggatcag aagaatagcc atcaagacta attgtgtaag
    26881 ctagcaagaa tgaccgatta aaaaatctgg ttattcctgc tggctttttt gattagttga
    26941 atttctctta aaatattcga aactgttcat tctgtattgc ttttgaccaa aatagagtgt
    27001 ataatcagag cttgtgagta gtctaattaa ttagataact ctccttgctc ttaggaacct
    27061 aagagccgaa agtccacagg gaggtgaata tcaatggaaa acacgaaata tgaaatcatg
    27121 tacattattc gtccaaacat tgatgaagaa gcaaaaactg ctttaatcga acgtttcgat
    27181 gcaatcttga aagataacgg agcagaagtt atcgaatcaa aagattggga aaaacgccgt
    27241 ttcgcttatg aaatgaacgg ttaccgtgaa ggtatctatc acatcgttaa agtatcttct
    27301 ccatcatcag caaatgctgt caatgaattt gaccgtcttg ctaaaatcaa cgacgatatc
    27361 attcgtcaca tgattgttaa agaagaagct taattgttgt ttcatgtgaa acaatagaaa
    27421 acgatgagag gagatgacta ttttgattaa taatgttgta ttagttggac gtctgacgaa
    27481 agatccagat ttgcgttaca cagcaagtgg tacggcagtc gcaacattta ctttggctgt
    27541 gaatcgtaat tttacaaacc aaagcggcaa tcgcgaggct gattttatca actgtgtgat
    27601 ttggcgcaaa tcagcagaaa ctttagcaaa ctatgctcgt aaaggaacac ttttaggtgt
    27661 tactggacgt atccaaactc gttcttatga taatcagcaa ggacaacgtg tatacgtgac
    27721 agaagtagta gctgacaact tccaattgct ggaaagtcgt tctgcatctg aaaatcgcca
    27781 acaaagcggc ggattccaaa gttctggtca atcagctggc ggatttggcg gaaacaataa
    27841 ctctaatcaa acatctcaat catccaacgg tatgcctgat ttcgaccgtg atacttctga
    27901 cccattcggc tcaagttcaa cgatcgacat ttcagacgat gatttaccat tctaaacgaa
    27961 tttgatagga gggaaataga atggcacaac aaagaagagg cggacgtaaa cgtcgtaaag
    28021 tagactacat tgctgctaac catattgaat atatcgatta taaagatatc gaattactaa
    28081 aaagatttat ttcagaacgc ggtaaaatct tgccacgtcg tgtaactggt acaggcgcta
    28141 aaaaccaacg taaaattact gttgcaatca aacgcgctcg tatcatgggc ttgctacctt
    28201 tcgttagcga agactaataa atcctaggaa ttgaatgact gatccttttg atcagctatt
    28261 caattccttt ttttctgtaa aaataacctc tattcgttta gtcgctcgaa tgacgaaagt
    28321 tatggtaaaa tcaaattagt catatatggg aggaaaatta tggaaaataa aaacaatcat
    28381 ttatccccga aacctctctt ccttatcctg tttatattaa tcgaagtatt ggtagtctta
    28441 attttaccat ttcgttttgt tagcgcttcg gtcattctat tgatgaattt atggggtgtt
    28501 cgctattggt tgaaaatgaa acgttggctt attttaaata aacgtgaaaa aatccagcag
    28561 gcttcgactg tagctgaaac caatctagct tatgtctcag aagtcatgcc tgtaggagtg
    28621 attgcttatc agccatcaag ccaaaaaatc gaatggctca atccattagc attgaaaatc
    28681 aaagatcaaa gtggattaac agaggaagaa ttgctagata ctttttttac tgcaaaagaa
    28741 aaaaagaaaa accaagtcta tctagcagat aaaacatttc agtttgatat ggatacagta
    28801 aagggtgttg tttattttaa tgatattacc caaaaccagt tgttaaaact ccatcagacg
    28861 aacagtcagc cggtgattgg tattttatct attgataact atgcagatgc cattgataaa
    28921 ttggatgaca aagagatttc ttatttgaac tctttcgtca ctacactagt ttctgactgg
    28981 atgaatgaat accatgtctt tttcaaaaga atcaacgcag aacggttttt cttcgtagca
    29041 cagatgatcg atctgaaaaa gatgatggat aaaaaatttg atttgctgga tcgctttcga
    29101 gcagaagctg aaaaacagag ctcgcctatc acccttagta tggggattgc ttatggctca
    29161 agaaccctcg atgaaatcgg tggagaagct cagaataatt tagatgttgc tttagtccgt
    29221 ggaggcgacc aagttgtcgt gaaagaggta aaagaaggag caaaacctgt ttattatggg
    29281 gggaaatctg ctaagaatgt caaacgcacg cgtgttcgtt ctcgagcgat gagtaccgct
    29341 ttgcgtcgga tttttgatga aacaggcgat gtgtttatca tggggcacaa gtttccggat
    29401 atggatgctt taggctctgc ttttggtgtc agttgtctgg cacgcttcaa ccagaaaaaa
    29461 gcttacatcg ttatcaacga gaacgagatc attccagatg tagaacggtg tcttgaagaa
    29521 atccataagc atcctgaatt agaaatgcaa ctaatctctc cagaacgtgc catagagctg
    29581 aaaaaagaag aggacctcct cgtgatggtg gattaccata aaccttctct atcgatctct
    29641 cagggcctat atgagcaatt tgataagatt gtgatcattg atcaccatcg tcgtggggaa
    29701 gagtttccaa gtaaacctct gctgacttat atcgaatctt cagcttcttc agcttcagag
    29761 ctagtttctg agctgatcca ataccaaagc agcaaaacaa atcgcttaga tcgttttgag
    29821 gccacattat tgcttgcagg gatggttgta gatacaaata gtttcaatgt ccgtacaact
    29881 gcacgaactt ttgacgttgc cagctattta aggtcttgtg gagcagacgc atccttagta
    29941 caatatttat tgagctcgga tttagcgaca tacttggaga tcagcaattt gattgctgga
    30001 agcgactatg tgacttctga tattgttgtg gcggcaggga cagacgaaaa agagtataat
    30061 agtgttatag cagcaaaaac agcagataca ttgctttcga tgatcaatat caatgcagct
    30121 ttcgtcatta caaagcggac agacggcttg attgggatca gtgccagaag taccggcacg
    30181 atcaacgtcc aaatcatcat ggaatcctta ggtggcggag gacacttcac aaacgcagct
    30241 actcaactgg aaaatacgac agttgaggaa acaaaacaga agctatttgc tgcaatcaaa
    30301 gaaaatatga atcaaatata tgataaggag taatccaaat gaaagtaatt tttttacaag
    30361 atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt tccagcaggt tatgcacaaa
    30421 attatctaat caaaaataac ttggcaaaag aagcaacgaa aagcagtatc agtcagttag
    30481 caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat cttggaagaa gctaaaaagt
    30541 taaaagaatt cttggaaaaa gaagatactg tagtcgaatt gaaggcgaaa gcaggggaag
    30601 acagccgttt gtttggttcg atcccttcaa aacaaattgc tgaagggtta aacaaacaat
    30661 acaaaatcaa attagataaa cgaaaaatcg aattagaaaa tccgatccgt tcactaggct
    30721 acacaaacgt acctgttaag cttcatcatg aggtgacggc tacgatcaaa gttcatgtcg
    30781 tacaagaata acaaatcttg atagcgatgt tcagctggta agtataacca gaaggaaaaa
    30841 agacaaaccg aaatttatct gagaaaacag ctgcagcaat gaaagcaaaa gctgcaaatc
    30901 gatttcagat tgatttcggt ttcttttttg ggaacagcaa aatagcataa agaaaacgag
    30961 tataataaaa gagaactatt tttttattac actttcggta cgaatgatga cttttctctc
    31021 attttcttgt aaaatgaagc aattgaagac aaggagcgat acgtatgaat gaagtgtggc
    31081 aggatcgaat cccaccgcaa aatattgaag ctgaacaagc ggttttaggc tctgtttttc
    31141 ttgacgcaga aacgatcatt gatgccatgg agtacataga gccaaaagac ttttatcgtc
    31201 gtggccatca aattattttt gagacaatga tcgagttaaa tgatcgtaat gaagcgattg
    31261 acgtggttac tgtcaaagac cggctggaac aagccaattt gttagaagat gccggtggtt
    31321 taagttatct ctcagacttg gcattagcag ttccgacagc agcaaatatc gtctactatg
    31381 cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat ccagacagca acaggaatcg
    31441 ttacgaaagg attcgagcaa ggagaagaag ttcaaacaat tttagatgat gccgaacgca
    31501 gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt tttatctatt gctgaagtac
    31561 tgaactccac gattgaaaat atcgatcgac tagctcaaaa caatgaagaa atcacaggtc
    31621 taccaactgg ctatcaaggt ctggataaaa tgacagcagg tttccagccc gaagaactaa
    31681 tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt tgcattgaat attgctcaaa
    31741 atgtaggaac aaaaacagat cgttctgttg cgatatttag tttggaaatg ggggcagaat
    31801 ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga ggccagccat ttacgtacag
    31861 gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc gatggggagt ctttctcgag
    31921 caaatatttt tatcgatgat acaccaggga tcaaaatctc tgaaatccgt gcgaaatgtc
    31981 ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt gatcgattat ttgcagttga
    32041 ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc agatatctct cgccaactga
    32101 aaaaactggc gaaagagctg aaagtacctg tcattgctct ttcacagtta tctcgtggag
    32161 tagaacagcg acaagacaaa cgaccagtgc taagtgatat tcgtgaatcc ggatcaattg
    32221 aacaagatgc ggacatcgta gccttcttgt atcgagatga ttattatgaa agagaaggcg
    32281 gagatgacga tgatggaaac gagccaaaaa atgacaacgt catcgaagtc atcatcgaga
    32341 agaaccgaag cggtgcacga ggaacagttg aactactgtt tatcaaagaa tacaataagt
    32401 tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg ttgtgagcct tgagcaatcc
    32461 cgagagctct cgtaagaaga tgcaaggagt aaccgcagga acgactagag tacttaattt
    32521 aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac agtccctttt tccgaataaa
    32581 cggtgttcaa tcagctgacc cttggtatta gctcagcaaa cgtcaaaata tgaggaacta
    32641 ttttgacgtt tgtttctcta atactcaggt caaaacgctg atcttacgac ctcttttcct
    32701 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatcatca aacatgagaa
    32761 gcagtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca caacctcttt
    32821 cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc ccaatcaaac gaaaaatatg
    32881 agaagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacaacct
    32941 ctgattcacg gtgttccaaa agtagcttct tcgaaaaata agtcgaaatt tcacaaaaat
    33001 ttgagaagca attttcgtac attccttctt atttcttgaa gctgaccact tctgttacaa
    33061 cctctttttt aataaccatt ctaaaaagat aaagttcgtt ttttaagcgt ttttataaaa
    33121 taaaaataaa aatcattcga ttttatttga aatatgtatc tattattgat acaatggttc
    33181 aggattaaat aatatagaac gaggtgttca catgtcatct gtagtagtag taggtacgca
    33241 atggggcgat gaagggaaag gaaaaatcac agactttctc agtgaaaatg ctgaagtgat
    33301 tgcacggtac caaggtggcg acaatgcagg gcacacaatt aagtttaacg gtgtgactta
    33361 taaactgcat ttgattcctt ctgggatttt ttataaagac aagatcagtg tgatcggaaa
    33421 cggcgttgta gtcaatccta aatcattggt taaagaatta gcttatttac atgaaaaagg
    33481 tgtagaaaca agtaatttgc gtatttctga ccgcgcgcac gtaatcttgc cttatcatat
    33541 caaattggac caattacaag aagatgctaa gggcgaaaat aagattggta caacgattaa
    33601 aggaatcgga cctgcctata tggataaagc agctcgtgta ggtatccgta ttgcagactt
    33661 attagataaa gaaatttttg aagaacgtct tcgtttgaat ctagatgaca aaaaccgtca
    33721 atttgtaaaa atgtttgatt cagaaccact tgattttgac gatatttttg aagaatatta
    33781 tgaatacgga caacaaatca aacaatacgt cacagatact tccgttatct tgaatgatgc
    33841 tttggatgca ggcaaacgcg tgttgtttga aggcgcacaa ggtgtaatgc tggatatcga
    33901 ccagggaact tatccttttg ttacttcttc taacccagta gccggtgggg taacaatcgg
    33961 aagcggtgta gggccttcaa aaatcaataa agttgtcggc gtctgcaaag cctatacgtc
    34021 acgtgtcggt gatggaccat ttccaactga attatttgat gaaacaggag aacaaatccg
    34081 cgaagtagga cgagaatacg gtacgacaac tggtcgtccg cgtcgtgtag gctggtttga
    34141 ttctgtagtt atgcgccatt ctaaacgagt ttcaggtatt accaacctat ctttaaactc
    34201 gatcgatgta ttaagtggat tggaaacagt aaaaatttgt acggcttatg aattagatgg
    34261 cgaactaatc taccactatc cagcaagctt gaaagagttg aaccgctgca aaccaatcta
    34321 tgaagaattg ccaggctggt cagaagacat tacaggatgc aaaacacttg cagaactgcc
    34381 agaaaacgca cgtaactatg tacgtcgtat ctcagagctt gttggtgtac gtatctcaac
    34441 attttctgtc ggacctgata gaacacaaac aaacatctta gaaagcgtct gggcgcaaat
    34501 ctaaggataa aaacgacgac tgaatcagga cgttcttacc agagcgagtc gaagtgttta
    34561 tctatacgat aataataact tgaagaggta gtgaaagaga cgtttagcct tattgccgat
    34621 aggctgtctc ttgaacactg tttattcaga ataagggact gtgacaaaaa ccgtgtcaca
    34681 gtcccttttt ccgaacaaac ggtgttcaaa agcttgcatc tacggcacta agcccaatca
    34741 gatgaaaaat atggagtgct atttttatct gattgttctt actgcttgat gcagagcagc
    34801 tttttcacaa cctctccact aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    34861 atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc ggagctaaac
    34921 ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca
    34981 aaaaacggca aaatatgagg aactattttt tccgtttttt tctctaatac tcaggtcaaa
    35041 acgctgattt cacaacctct gattcacggt gttccagaag tagcttctac ggaaataagc
    35101 cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    35161 gaccacttct gtcacaacct cttattttga ttagaacttg cgaaaataaa ccggttaaag
    35221 tgtgtctagc gcaagagatt tgctataata gacgataagg gtaaaatgta acgagaaggg
    35281 gttgaaaaat gtgtatcaaa ttgtaacaga ttcttgttgt gatctgccat accatgtatt
    35341 agcaaaagaa aaagttgatt ttttatcaat gaatctccaa ttgaatgatc aagagttagt
    35401 agatgacctc ggaaagacat tcaattatga ccgctacatg aatcagctga aaaatggtgc
    35461 gatgcctacg acatcacaaa tcaatgttgg ccgatattta gaatttttcc gttcttatgc
    35521 ggaaaaacag attcctgttt tatatgtggc tttttccagt ggattaagcg gctcttacag
    35581 tagtgcgctt caagcggttg aattgctgaa ggaagaatat gaagaccctg agatctatgt
    35641 ttttgataca aaagctgcaa gtattgggga agggctttta gtattggaag ctgtaagatt
    35701 gaaaaaagaa ggtaaaagca tcgaagaaac cctagagtgg ctggaaagta attatttgaa
    35761 agtccattca tgggtaacag tagacgatct gaaatatcta gaacgaggcg gtaggatttc
    35821 caaagcaagt gctactcttg gcagtctatt gagtgtaaag cctattatta cggtggataa
    35881 agaaggaagg ctgacgaatg ttgacaaagt tcgaggaaga aacaagtcga tccagacaat
    35941 cgtcaatgag acggtaaaaa caatcgttgc accacttgat caaatcgttt atgtggctta
    36001 cgctggggat ctagagagcg cggagaaggc aaaacggctt ttggaagagc agataaagat
    36061 gaaagatgtg aagctttatc ccttaggacc aacgatcgcc agtcacacag gctatggctg
    36121 tatcgcgatt ttttcgatgg gaatcagtcg ataaaagcag taactcattc tgtaaaaaag
    36181 cccagaaatt gattgtaaca aaaacagcga ctcttaaaag atagatgagc atcctatctc
    36241 ttagaggtcg ctgttttttt attttttaag ctaaataggc cattatgccc ataactcgtt
    36301 caattgtttt tgaatatctg cattttccaa aaattcatca tatgtcgtat cagctcgatc
    36361 gatcacacct ttatccgaaa cggcaatgat gcggtttgcc aatgtttgga taaattgatg
    36421 gtcatgtgac gcaaataaga ttgagccagt aaatgccatc aagccgtcat tcaatgctgt
    36481 gattgattct aagtctaaat ggttcgttgg atcatctaat acaagtacgt tagctttcga
    36541 aagcatcatt tttgaaagca tgcaacggac tttctctcca ccagataaaa cattcactgg
    36601 tttcaatact tcttcaccag aaaacaacat tcttcctaag aagcttctta agaaggtgtt
    36661 atcatcttct tctttacttg catattgacg caaccagtct aaaatcgtta gatcagtatc
    36721 gaattctttc gttgtatcct ttggtaaata agaattactt gttgtcacac cccaacgtac
    36781 tgttccagta tctggcgtaa tttcacccat gataactttg aatagcgttg ttgttgttat
    36841 atcattttct gcgacgaaag cgaccttgtc ttctttgctc aaattaaaag atatatcatc
    36901 taaaattttc tttccatcaa tcgttacact tacattttct acctgcaata agtcgtttcc
    36961 aatctcacgt tcaggtttga atccaacgaa tggataacgt ctagaagaag gttggatatc
    37021 gtctaaagta attttttcaa gcattttttt acgtgaagtt gcttgttttg attttgatgc
    37081 attcgcacta aagcgggcaa taaattcttg gagttctttg atttgttctt cttttttggc
    37141 atttgcgttt gcttgaagtt tagccgctag ttgactagat tccaaccaaa aatcataatt
    37201 tcctacataa agtttgattt taccaaaatc tagatccgcc atatgtgtac atactttgtt
    37261 caaaaagtga cggtcatggg atacaacgat cacagtattt tcaaaattga tcaaaaattc
    37321 ttccagccaa ttgatcgatt ggatatccag accgtttgtt ggttcgtcta gcaaaagaac
    37381 atcaggttta ccaaatagtg tttgtgctaa taacactttt accttttgtc caccagttaa
    37441 ttcactcatt tgctgatggt gcaattcttc agggatgttc agaccttgca gtaaaacagc
    37501 tgcttctggt tcagcttccc atccattaag ctcagcaaat tctccttcta attcagctgc
    37561 tcgaatacca tcttcgtcag tgaaatcctc tttcatgtaa atcgcatctt tttctttcag
    37621 cacttcatac agacgcttgt gtcccatgat gactgtttca agtacagtat agtcttcata
    37681 gtcgtaatgg ttctgtttca atacggccat ccgttcatct ggtcccatag taacggaacc
    37741 tgtggaaggc tgcagatccc ctgataacac tttaaggaac gttgatttac ctgcaccatt
    37801 tgcaccaatc aagccatagc agtttccggg ggtaaattta atattcacat catcaaatag
    37861 tttgcggtct gaaaagtgca aactcacatc atttacagta atcaattttt ttcctcactt
    37921 ttatcattat tagttttgac aagtcttata gaattatata ggggaaaggt aaaagattca
    37981 aggcaatcga acattctttg tgtttcacgt gaaacagaat gaaaaaaata aaaataaaag
    38041 cggagcgaaa atagctacta taggatttat tagtttgcaa cagcataatt tttgacgtta
    38101 tttataagag ggagtactat aaaactgtaa aagaaatgaa agaaggattg aaaatggatg
    38161 aaaaaagaac catcgactgg ggctcattga ttttgggaat tttatttgta cttgtctcat
    38221 tgttatcatt tcaagaccct gtcggaaatt tagttgcaat tgtagtagtc tttgcgattt
    38281 ttgcctttat taaagggata tttgaactgt ttgtacggaa cagattaaaa gagctaacag
    38341 gatataaagg aaagatgccc ttaataatcg ggatcatcga tattctagtt ggcgtctttt
    38401 tcttattcaa tattggtgca ggagtcgcag cattgccttt tgtttttgca gtatggttta
    38461 ttgctgattc agttttagcg ttatttactg ctgacttggc aagaggagta agtgaaggat
    38521 attattggtt tacgatcatc gtcaatattt taggaatttt attaggaatt ttcttattgt
    38581 ttaatccaat ttcttctgcg ttgacattaa gctttttagt tggattctat tttatgttat
    38641 ttggtatcac acatatcgtc tatgcattta gataagtaaa atcaaacaaa taaaaaagcc
    38701 ggcttccgat tacagtttta ctgttgtaat tggaagccgg cttttttatc atggacaaat
    38761 atgcacgagg tgatttttag gtgtcgagac aaatttatgc aaaaatgtga gaagctatat
    38821 acacaaattt tatcttaata ctcgaagcat agcgcagttg tcacaacctc ttttcatata
    38881 attcattcaa tactggcaaa tcatggactt catatttttc tttgaatgct tggatttctt
    38941 cttcagaata ttttcttgga tctaaatgca gatcttctac tggcaatggg cgcttgttat
    39001 tgatcttcac atcgattact acaggacgat ctgattttgc agctgcatca aaggcaaatc
    39061 gaagttgcga gtattctgtg actgtaaagc tatttgcccc gagcgcttct ccaactttac
    39121 cgaaatctgc atcttctaaa aatacaccaa atttcttttg ttccgtatct tcttgttctg
    39181 cttcgatgaa accaaaagaa tcattcgaaa agacaacatt aataattgga agctgatatt
    39241 tgacttgtgt aatgatatct tgcatgacca tagcaaaccc gccatcacca cttaaggtaa
    39301 atacttgttt ttctgggtag cttagttgtg cggcaattcc tcctggcaca ccattaccca
    39361 tagttgcgaa ccatccagat gtcgtatgtt tttgtttacc gttcatattc aataaacgaa
    39421 tagaatggat agtcgtgtta ccgacatctg tgacgaaaat cgcatcgtct tcagcaatac
    39481 gattgatttc tttatagaca gcctctggac gaatcggttc ttcctctcga tcttcaaaag
    39541 atttcaacca atttacccat ttttctttat tttcttgatt tgctaggtac cagctatcag
    39601 cagggcgagc atcccctaac tcgacaagtc tttgcaaagc tgtgtttgca tcaccgagga
    39661 tcgacaaaga tgtttcatgg cgtcgtccaa attttgacgc atcgatatcg atttggataa
    39721 attcagcagt tggattaaaa aatgcacgtc caaatgggaa gtcgctaccg acaaataaga
    39781 tcagatcggc ttctgccaat gcttcatttc cgggtttagt agcgacacgt ccagcaaaac
    39841 caagaaagtt ttcataatca tcaggaataa ttccttttgc tagaacggaa gcgacaacag
    39901 gaactgaaaa atgttctgag aattctttga tttgcggaaa tccgtttcgc gttccttgac
    39961 caacataaag aactggtttt tttgcttttt caagataagg caaagcagct agcaaatctt
    40021 tttcttctgg taatatcaca cttgttttgt gagtgtgagc ggtggagaaa ggttgttcgt
    40081 caatttcttc aaaaccaaga tcaacaggga tagtcacgat agccacgcct ttatgctcat
    40141 aggcggcttt aatggcttca tctactacat gcggaagact ttgtggtgtc atgactgtac
    40201 gattgtatac gctaacatct gcgaaaatag gattttcatt tagttcctgg aaagagttat
    40261 agttcatgga tgtagaagca acttgtccta agaacgctac tagaggcaca tggtccatct
    40321 gtgcatcata caaaccattg atcaaatgtg tcgcaccagg tcctgcagag ccgaagacag
    40381 cgccgacttt ccccgttaat tttgcatctg cagcggctgc gagtgcgccg acttcttcat
    40441 gacgaacttg aatgtagttg atttcggttt tttcttgata tagcgcatcc ataattgaat
    40501 tgaaagatcc tccgggaatg ccgtaaatat ggtcgatacc ccagctttca agaactttta
    40561 ccattgcgat tcctgcattg atttttgaca tggtgtttca ctcctttagc aatgatgata
    40621 cataaatttt ttgtatctag agtatagaac tttttatcga aaatcccaac taattcgtct
    40681 tttcacaaaa atgtagtcat gtttttggga atatgctaga gtagtacata gatagattca
    40741 agagagggga atggcatggt tgaatggcta ttgagacttg aaccttggca gcaagcactt
    40801 gctgggacag cttttactta ttttatgaca gctcttggtg ccgggttggt ttttttcttt
    40861 aaagaaatca aaaaagaagt gctaaacttg atgttaggat ttgcttctgg tgtcatgatt
    40921 gctgcaagtt tttggtcttt gttagatccg gctattacaa aagcagaaga aaatggagat
    40981 attgcttggc tagttgtaag tattgggttt ggtttaggcg gtgtgttttt atatatggca
    41041 gataaaacac tgccccatat gcattttgga ccacaacatg aagcagaagg attgcctacc
    41101 catttaaaac ggacgatctt gcttgttttt tctattacat tgcataacat tcctgaaggt
    41161 ttagccgtgg gggtagcatt cggtgcagca gcaactgcgg ataatccgac agccgctata
    41221 ttagccgctg tttcggtggc gctaggtatt ggtatccaaa acttcccaga aggagcagct
    41281 gtatcaatcc cgttgcgaca agaaggtctg agtcgtaaaa aggcctttgt ttacggacaa
    41341 gcttctggaa tcgttgaacc aattgctgga gtgattggag cgctactagt aacgaaagtg
    41401 gagctgctgc ttccttatgc acttgctttt gccgcaggag caatgattta tgtcgtggtg
    41461 gaagaattga tccctgaagc acagcagaca ttgtccagca aacatcattt tgcagttttt
    41521 ggcgtcatga gtggatttat cattatgatg attttggatg tggcattagg ataaataaaa
    41581 aaaatatata tgatatatca aatagggccg cgaacttata tcgcgaccct atttttttag
    41641 taagttatat cataatgaag tgaatggtat catacattaa taatagtttt ttctatgtgt
    41701 atttcctttg atccagcgaa caagccagat gataagtgcg attgggatta gaactttgac
    41761 cgcaaaccat aaaagcccgc ttaggacact gacaaaaaga ctgatgacga agctagctgc
    41821 aattagcact aaaagaacca tgaagatatt taagaacata tttttttgac gcatacaaaa
    41881 ccctcctctt gttcattctt tactatagaa aaagtataac agggattgtt taaaaacacc
    41941 tccaactgaa gactgatttt tacttcatct acttaaatga tcaaacaatt gctttttctc
    42001 tggtttatca tctaaaatag agcggaaaag gaggagaata tcatgcctaa aaatcgtctt
    42061 gaagcattta cagatgctgt aatcgcgatt atcatgacta ttttagtttt agaacttcat
    42121 gaacctaacg gagacacgtg ggaggcgctc gctgcattag gttaccgatt ttttatctat
    42181 attgtcagtt ttatcatttt agcgatttat tggaacaatc atcatcattt atttcaagtt
    42241 gtacataaga taaatggacg ggtcttatgg gcgaacagtt tctttgtttt tgcgctttcg
    42301 ctttttccgt ttgcaacttc atggatcagt gagtacatca atagcctggc tccagaaatg
    42361 acttatggtt ttgttacctt gaccgctaat attgcgtatt atatcttggc aaaagaactt
    42421 gtcaaagcaa ataaaggcca cgagaaagca ttgaaggcga tgttgaataa ttacaagaaa
    42481 tcgtatctat cgattggttt gaatgtgtta ggaattgtct tagggtattt agtagcgcca
    42541 gtagccgtgc tgatcgcaaa catcgtgatt ctagctgctt ggatcatccc aagtcgtacc
    42601 atcgaatcac aatatcggta aaatgatccg tgtttaaaat ttaagagaaa aatatagaaa
    42661 aaataaataa acgatcaaat atccgagtga tactagtatt caaagaggga tgcagatgga
    42721 tcaaaaaaga attcatgtag gactgattac tagttttgtc acgatgctta tttatttgga
    42781 tcaaggtcat ttgacttgga cagtagttgg tgtcgtagga tttttgacat tattcacagc
    42841 aataaaaaaa taaacatctt tatgtaatct gatgttctat ctatttaaga aaagcatgtt
    42901 tacagcagta tgattctgtt gtaaacgtgt ttttatttgt ggaaaattta atttttagaa
    42961 aagtgtatcg aaaaatagat agttagtttt tccacagtgg ataacttcgt aagagataaa
    43021 aataaaaaat tagtcaaaga agggcgtata gatttctttt gataggagat atacaacaga
    43081 aatgaataaa tattaacgtg ttttcaaaca gtataaaacg gtgtggtttt tggaataaaa
    43141 aaataacata tctcatttta cacttttgtt attttatact taaaaaataa cgacacagaa
    43201 aataccgtgt ctcagatttc tctactactt ctaaaaacta ctgttttttt cctttgaaat
    43261 gccttcaaaa agtaggatca ggcagaatat actggcagaa tcaaggagaa gggagcctaa
    43321 ataaggagaa aaaccataga aaatataaag taattactta taaaacaagc tttttataga
    43381 aaaataatat acctgagtag ggtataaatt aagacggaat tcctttctag attgagatat
    43441 tgactaggaa gaatgttaag attttaagcc tttaagctaa aaaaaaaccc tttttgttaa
    43501 ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt
    43561 agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga
    43621 tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac
    43681 agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat
    43741 aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca
    43801 taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat
    43861 accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt
    43921 tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac
    43981 aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa
    44041 agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag
    44101 cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac
    44161 ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca
    44221 taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt
    44281 ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg aaaagttgct
    44341 atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt ctccaagttg
    44401 tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac taggtaatgc
    44461 tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac taggaaggag
    44521 ggatagattg ttcaagactt attttcgttt caataaaata caagataatc cttttcgtat
    44581 catgaatctg atgctactta tctttattgt attatggtta ggttacataa atcatgattt
    44641 tttgatttca agtttttcat cattagggat ctttactttt ttctattatc aaaacatacc
    44701 gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg ttgctttcgt
    44761 tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag ctttggttgc
    44821 tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg gcttgttttt
    44881 tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat taccgatcgt
    44941 ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt
    45001 actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa gatttcacga
    45061 agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc tttccgttta
    45121 tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat gtgcatcgat
    45181 tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc tgattggatc
    45241 gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc agttgcagac
    45301 catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta gaaattatgc
    45361 agttgctaat atttttctta atccgatggc gttgatgcta tctacactta ttcgtggagc
    45421 atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa gtttcatcgg
    45481 tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg ttgttcgtca
    45541 aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag atttttgtca
    45601 caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa attcacacaa
    45661 aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa attttgttta
    45721 tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga
    45781 tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat
    45841 accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg
    45901 gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc
    45961 ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg
    46021 agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag
    46081 acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc
    46141 aaagttggaa aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa
    46201 tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg
    46261 accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg
    46321 agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt
    46381 gcctttctct tcatattcct tccgatgcag atttatctca gaataagata agagatagtt
    46441 acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt
    46501 acacagatac ttggttacta tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa
    46561 tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag
    46621 tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt
    46681 tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta
    46741 ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa
    46801 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
    46861 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    46921 cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac
    46981 gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa gagggccttt
    47041 tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta
    47101 gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca
    47161 caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg
    47221 gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat
    47281 atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa
    47341 aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc
    47401 aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca
    47461 tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat tccagttata
    47521 tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt
    47581 tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac
    47641 ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg
    47701 ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac
    47761 cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc agctcactcc
    47821 atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa
    47881 ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta ctattgctag
    47941 aataacatca aggaataata agttttggtt ttcagataat agtcgataag ttgtgtattg
    48001 gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc actattacaa
    48061 gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa atgaatagtt
    48121 gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt caatttgtga
    48181 ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat tgtagtgttt
    48241 attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa
    48301 catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg
    48361 gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat
    48421 atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta
    48481 gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt
    48541 gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata
    48601 ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa aatttactag
    48661 atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag
    48721 ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg
    48781 aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa
    48841 ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta
    48901 gaagagtttt tggattatgt tagtatgcaa cagtaaggga gttcttatta tgaaaaattt
    48961 tgacacatat tatgttattg ttttttcgat atctttgctg ttaatattac taactttaat
    49021 gcagtataaa gagtatccag agtattttat tttaactttt gtatatttag taagtagcta
    49081 cacacttcta tataaattca gaagttcttc tagagtttct aaagtttgtt tcttaattag
    49141 cttattgatt ctataatatt tttgatttat ataggaaatt aaaaatatta tagaatcaac
    49201 actcagagaa cgggttggtt tttaatagat ttaataagta ctgataatga aatttctcct
    49261 ggttgggaaa acgaagttgg ttaccctgta gaagaatttt ttcaagatta ctttgcggag
    49321 tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt catcaatcaa
    49381 tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc cattatttct
    49441 tcagggatta gtgactatat tgatatgagt ggtgaaatca attatggcga taaagacaaa
    49501 attgtacgca tgataaaagc ttttatcaaa acttttgatg tgaaggcaga tcttaagttt
    49561 aatgatactt acctagtagg aaaattaatt aatatccttg ggacagagaa agatacggaa
    49621 acgtttattt cctctttgtc tatgttgttt acagataagc atttttcaac tcagcttggt
    49681 ggagaggaac tagtggaaat cttttaacca ttacgcccag cattaataaa agtatatcaa
    49741 aaaagtactc ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa cactattttt
    49801 aatgttaatt atagatagag atgaacgcgt catatggtta aaagaatgaa aaagtcaaag
    49861 atacactgct atatttcact aaggatatag cagtgtatct ttcatgtcgt ttcactggca
    49921 gttttttata aaaggaggtg tgcatttcag ttgcttttct ttggtagtta gttgaatcgt
    49981 ttaagaatta acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa aatctttgat
    50041 ggttccatct ttatttagct gtgcctgttc aaatactgtt ttgtttttac cgaatggatc
    50101 aattgcaata attgttccga tccccgcacg tttggcagca agtaatcctg agtaagcatc
    50161 ttcgataaca agacaatcag ccggagctaa gctcagtttg tctgccgctt taagaaaaat
    50221 atccggttgc ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt cccagtcaaa
    50281 ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga cagtagccgt
    50341 ggcaatcgtt aatggaatct gttggtcagt cagctggtca agagtcgctt caagcccttt
    50401 tgtcaactga agctcgtttt cattttgcaa gcaaagcata cgataatagc tttctttttc
    50461 ttctgacaat acggcgatct cagaggcggt caaatgctct gaaatgaaat gtttcaaaat
    50521 ctccgtattc gtccgaccat gaatattttt taaaatatcc tcgtctgata aaccatcttt
    50581 tgtatgatca tgtatcatat gcagccaagc agcttcatgc agatgagagt ccaaaaacat
    50641 tgttccgttg aaatcaaata taatcccttt catagttacg ctcctatagt ggtattttcg
    50701 gttgaatcag caatttgcag atcgacatat tgtgcatagt gctcttgaac ctcccgcgag
    50761 atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac ataaaaatca
    50821 tctcgattga attttttatt atcaacgagt agatatttct tacgggaatt atttagagcg
    50881 atctgctgtg cttctccttc ttcggtactg taagtagaga tcgcttcgtt gtgaacgcca
    50941 tttgaactga taaatgcttt tgtaaacttt agtcttctta aattgtcgtt ggtgatcggt
    51001 ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa tcagtagaat ttctggtcca
    51061 tctgcttgag caagaatatc aaaaactgga taattatttg taatgatccg aattttcttg
    51121 ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa aatcgtttct
    51181 ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt ttttttcttg aatctgcaga
    51241 gtggcttttt cagtgtgaga aagttcttga tctaaagtgt aggaaagact ttgagcccct
    51301 ccatggacac gcagcagctt gcccgatttt tctaattcat ctaaatctct gcgaacggtc
    51361 atatcagaaa catctaattg attgatgatt tcattaacgg taaggattcc ttttttattg
    51421 accatttcta cgatagtaag caatctttct tttttcaaca ttgtcttcac ctctaattta
    51481 aggataatct atcatataaa cggacaacag tcaatctaat tcgaacaaaa gtaaacattt
    51541 agggtggttt ttatgtttgt tacaatagat tttattgctg ttttctggtt tttatgatta
    51601 tttgtgtttg tttttgttga caaatgtttt ggatgcgttt acaatgcaat cagttagaaa
    51661 gagcgaaaac tgattataga tttgaggaga gatgtacatg cttaaattac cagaagattt
    51721 tatttttggc ggagccacag cagcctatca ggttgaagga gccactaagg aaggtggcaa
    51781 aggcgctgtt gcttgggatg actttttaga ggaacaaggg cgattcagtc ccgatccggc
    51841 aagtgatttt tatcatcaat atgcaaagga cattgaattg tgtgaacgtt ttggtgtaaa
    51901 tggtctaaga ttatccattg cttggagccg catttttcct gatggagctg ggaaaccaaa
    51961 tccagaagga atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa gaaacgtaac
    52021 gccgtttgtg acgttgcatc attttgatac ccctaaacgt ttgtttgacc atggagattt
    52081 tttgaatcga gaaacaatag aagcatttgt ttcctatgcc attttttgtt ttcatgaatt
    52141 caaagaagtc aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa ccaatcaata
    52201 tttgttaggt gttttcccac caggaatcaa atatgatttt acaaagatcg tcgcctgtct
    52261 tcataatatg atggttgctc atgcccgtgt agtgaactat ttcaaagaaa acgaacttcc
    52321 gggtgaaatt ggtgtagttc actcattaga gacgaaatat gcggcaacag acgcgccgga
    52381 agataagcat gctgcctttt tagacgatgc attgtcgatc cgtttcttat tagatgctac
    52441 ctatttaggc tattattcaa ctgaaacctt gactgctctt gatgagatat gtgaagcaaa
    52501 tcaggccagc tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg
    52561 taatgactac ttagggatca atcactacca atgccatttt gtcaaagctt acgatggaga
    52621 aaatgcaatc catcataacg gtacgggtga aaaagggacc tctgtttata aagtgaaggg
    52681 aatcggagaa cgtatttata aagaaggaat tcctcgaact gattgggact ggctgattta
    52741 tccagaaggt ctatatgact tgttgctgcg aattaaaagc gattacccac attacaacaa
    52801 aatctatatc actgagaacg ggatggggta taaagatcag tttgaagatg gcattattat
    52861 ggatcagccg agaatcgact atttgagagt ctatttggaa tccttaagca aggcgataac
    52921 agccggggta aatgtgaagg ggtatttttt gtggtcgctt atggatcttt tctcatggac
    52981 aaatgggtat aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc agaagcgtta
    53041 cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa accaaaacga ttatttaata
    53101 agtgataatg gaggaagaaa agatgaataa agaggaatta caaatgcttg gttttgagat
    53161 cgttgcatat tcaggcgacg ctcgcagtac attattgaaa ttattgaaag aagtaagaca
    53221 aggaaacttt gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt taacccttgc
    53281 acataattcg caaacaaaaa ttttagcgga agaagcatct gggaaagaga tggagatggg
    53341 attcattttt atccatgggc aggatcattt aatgaccact ttattgctga gagatctgat
    53401 tcaagacttt attgtactgt acagacagaa tcaaggatag gagaagaacg ctatggatgg
    53461 aatcattaaa caaatcgaaa aagggaaacc attttttgaa aaaatctcac gtaatagtta
    53521 tttaggagcc gttcgtgacg gatttttaac agcgatgcca gctattttat tttcaagcat
    53581 tttcattttg attgctgcgg ttcctgagat ttttggttac caatggcctg aatctgtctc
    53641 cacatggcta tggaaagtct ataattattc gatgggtgtt gtagggttac tcgtttcagc
    53701 aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat
    53761 caacaccacc tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc
    53821 gattgaaggt ggattttcaa cggaatatat ggggacaaag ggactactgg cttcctttgt
    53881 cgctgcattt ttaacagtta atttatataa gttttgtgtg ctgaaagata ttaccattaa
    53941 aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc cgagatattt ttcctttctc
    54001 tttttcagta tttgcggccg ttttaattga tttaggcatt cgacaaatct tccaaatctc
    54061 atttgcagaa gcaatcatta cggtgctgca gccgctattt acagcagctg acgggtactt
    54121 aggaatcgct attatctggg gagcgatggc gctgttttgg tttgtcggtg ttcatggacc
    54181 gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca
    54241 attgttcaat tccggtgaac aagcttctaa agtactgacg gtgggtttag ggaattttgt
    54301 aggaactatg ggaggaactg gggcaacatt ggttgttcct tttatcttcc tactctttgc
    54361 taagtctaag cagttgaagg cagttgggaa agcttccttt attccagtaa gtttcgctgt
    54421 caacgaacca ttgctatttg cggcacccat gattttgaac ccttattttt ttattccatt
    54481 tttgttaaca cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg tattgggcat
    54541 gaacagcttt atgtacgttt tgccttgggc aactccagca ccgatcggtt tggttctagg
    54601 aacgggcatg ggtatcttag cttttgttct agcagcggtt ctaatcgtcg ttgacttcct
    54661 gatctatcta ccattttgca aagcatacga tgctgtctta gtagagaaag aagcacaaaa
    54721 agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt
    54781 ggcagcgagt caagttatca gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc
    54841 tggcgcaggg acaagtgcga tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa
    54901 agcaccgatc agtgcaatgg cgggtgctta tggttctcat tacgaaatgc tgcaggattt
    54961 tgatatggtg gtcttagcac cgcaagtcaa ttcttactat gatgatatca aagaagacac
    55021 cgatcgctta ggaatcaagc tggttgcgac aaaaggagca gaatatatcg gattaacgag
    55081 agatcctgaa aaagctgttc agtttattct gaaccaattt caataaagga ggagccataa
    55141 tgaaaatggg aatttggttg cgtaaagctg ttttatttgc agctgtgtta ggggctttgg
    55201 gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga gcagatattt
    55261 cgatcctgtc agatatggaa aaatctggtg caaaatatta tgaagacgat atttcaaaag
    55321 atgccttaga gattttaaaa aataatggtg tcaactatgt tcgactgcgt ttgtggcagg
    55381 atccttataa tagtaatgga gaaagttatg gagcagggac aaatgatctg gaaacgacga
    55441 ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt gctgctggat tttcactaca
    55501 gtgatttttg ggttgatcct gggaaacaga atcttcctaa agcatggcaa ggtctgacct
    55561 ttgaagaaat gaatacggca ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc
    55621 agctggacgt ttatcctgat atggttcaga tcggcaatga actgaatagc ggaatgctct
    55681 ggccttatgg caagtcttgg ggagaaggcg ggggagaatt tgatcgattg gctgcttttt
    55741 tgaagtctgg tatccaagct gttcgagaca ctcaacctaa aacaactcca gtaatgctcc
    55801 acttagctga tggtggcgat acaggagcat ttacttggtg gtttgatgaa atcacgagtc
    55861 gaggtgtgtc atttgattta attggagtgt cgtattatcc gtattggcac ggttcaatga
    55921 atgaacttca aaccaatatg aatgcgatca gccaacggta tggtaaagaa gtgatcgtag
    55981 ttgaaacagc ttatgggcat acaacagcga atgccgatac tatgccaaat gcttttggtg
    56041 aagccgaagc agctgcgggg ggatatgaac caacaccgac aggacaagca gagtatttgc
    56101 tggatcttgc cgatcggatt caggcagtac cgaataatcg cggggcgggc ttcttctatt
    56161 gggaacctct ttggtacaac ggaaatgtga gttgggcaac acaagcaggt atgaattatc
    56221 tcagtgttca atccactatg ggaaacgaat gggacaacca agcgatgttt gattttgctg
    56281 gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata aatctagtta
    56341 aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg caaacctggt
    56401 ttcaacaagg aaccgctaag ggtacgatca aaacggaagc gggcactttt tttggggact
    56461 acaaactaac tttttggcag gatcaagcat acgaggcatc agcttatcaa aatgttagtg
    56521 gcataagcaa aggaacttac acattgagtg cctatgtaat gtctggggga gagtttgata
    56581 ctaatcagtt atatatcaaa aattatggtg gagcggaact aaatactgcg gttgttaaaa
    56641 caagtgaatg gacaaaaatt acaatcaaag acattccaat tacaacaggt gtttgtgaaa
    56701 ttggggttta tagcaaggct tcaggaaatg cttggacgaa cgttgatttt gtttcattgg
    56761 tgaaacaata gactgcggat cgtttcgatt caagatcgga ggttcccttc agccacatcg
    56821 ttagcgtaga aggcaggaat cgaacctgca cctcgccttt gccaaaaata accagaaacg
    56881 atttccagtc atttttacca aaacgagtaa agcaccagac ttcatctaca ctttgtatta
    56941 tgctaaagag agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca
    57001 gataacataa agtttgttta agaaagggta accttagaat tccgtcgtga atatgatttg
    57061 agcacttaga tggatagaaa aaagttctcg gaaaacagtt cgtttttcga gaacttcttt
    57121 ttatgataag aatgaaacaa attaacttat attatagtct cttttggtag ggaaccatct
    57181 tatttttaat gaaaatcaag cattttagct attgaagaat gttgatttat agggattccc
    57241 cctccatatt atgttggctc tttattccca ctccactgtt gctggtggtt tagaagtgat
    57301 gtcatacacg atacggttga cgtgggctac ttcgtttacg atacgtacag agattttttg
    57361 caagacttcc catgggatac gggcaaagtc agcggtcatt ccgtcgattg aagttacagc
    57421 acggataccg actgtgtagt cgtaagtacg tccgtcaccc ataacaccga cagagcggat
    57481 ccctggaaga acagtgaaat attgccagat atcacggtca agtccagcag ctgcgatttc
    57541 ttcacgcaag atagcatcag attcgcgtac gatttctagc ttgtcttcag tgatttcgcc
    57601 aagaacacga ataccaagac caggaccagg gaatggctga cgccaaacga ttgaatcagg
    57661 catgcctaac tgagtcccta attcacggac ttcgtctttg aataatgtgt ttaatggttc
    57721 gatcaattca aattgcatat cttctggcag tccacctaca ttatggtgag atttgatcgt
    57781 ctgtgctgtt tctgtaccag attcgataac gtcagtatac aacgttcctt gtgctaggaa
    57841 agagacgcct tcttcaccag caagttttgt tgcttcgtca tcaaaaacat atacgaattc
    57901 attcccaatg attttacgtt tttgttctgg gtcagataca cctttcaact tgtctaagaa
    57961 gcgatctctt gcattgactt tgatgatgtt caagccgaat tttccgccta gactttccat
    58021 tacttgttcc gcttcgcctt tgcgtagcag accgtggtca acaaagatag atgtcaattg
    58081 gtcaccgatc gctttttgta aaagaacccc aacgacacta gaatcaacgc cacctgaaag
    58141 accaagcaag acttttttgt caccaacttg ttcacggatt ttttctactt gcatatcgat
    58201 aaagttatcc attgtccagt taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag
    58261 caattcgatt ccgtattcgg aatgacgtac ttctgcatgg aattggatac cataaaaatt
    58321 acgtgttgtg tcttggatag aagcaatcgg acaatctttg cttgttccga ctgtttcaaa
    58381 gccttcagga actttagtta ccaagtcacc atggctcatc catactgttt gttgttttgg
    58441 tgtttcttta aacaagacag cttgatcgct tgttacttct aacaaggctt tcccatattc
    58501 gcggttttta gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt gcataccata
    58561 gcaaatccct aatacaggaa tacctaaatt gaagatctca ggatcgatcc caaatgcatt
    58621 ctcatcatat acactgtttg gtccgccaga aagaatgatt ccctttggat tcatcgcttt
    58681 tacttcttct gcagtaatgc ggtggctcaa taattcggaa aagactccaa gatcacgaat
    58741 acgtcttgtg atcaattggt tatactgact accgtagtct aatacgataa tcttttcaac
    58801 gttcgtcata tcggcaacgt ttgtcaaatt tattacccct atccctttaa attttatctc
    58861 aaaatagtca aactatttta aaatgctaat attttcgcat gaaaatcgaa tcgatgatat
    58921 gattttcagt tttatggaga attatatcgg ctcttcctcg tgtaggcaag atatattcct
    58981 gtaaattttt caaattcaca tttttccata catcttttgc cattttaaag gcttcttcac
    59041 gatctccgat agcaaaacga taataataat tctctggatt ttggaaagcc gtatccagta
    59101 acgcaccaaa acgatccaaa taccattttt cgatcagcac gggatcggca tcgacataga
    59161 cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat
    59221 tgatcccctc aacgatcaag atatcaggct gctggatcaa ttcgtacttg tctggaatga
    59281 tatcatacac atcatgtgag taaacaggta cttttatctc ttcctgacca gattttacac
    59341 ggttcaagaa atcgatcagc atctccatat cataactttc aggaaatcct ttacggtcca
    59401 tgattccacg ctcttttaat atacgatttg gataaagaaa tccgtcagtc gtgatcagct
    59461 gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg gctgttgtac
    59521 tttttccaac ggctacactt ccggcgatcc cgataataaa aggcggtact tctacgtatt
    59581 tatgaagaaa taaaccttta ctcaagaaca aagattcaaa ttctttcata taaagataaa
    59641 tcagatgagc aagaggtaca tagatatctc gaacatcctg catggaaatg cggtcattca
    59701 aacttttgat attatctagt tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt
    59761 ctcgccattc ttctcttgcc acacggtagt agttcattgg atcattcata ttagcctcct
    59821 gcgttctccc atcaatggaa ctattttaac acaaaataaa cgcaaacccc accacttaag
    59881 agagattgcc gatctttttg tataatcttt ataatagaaa gaaacggagt cttattcttg
    59941 acttgcgcac ggatacataa aaggatagac tgttgtcatg aaagaaatga gaaggatgga
    60001 agacatgaca aatcattact attctgaacg cccagatacg gcgcacgatt ttgaacaatg
    60061 ggcttttgag ttaaaaggca gaacctttca atttgtaaca gattctggtg tattttcacg
    60121 tgaaacagtg gattatggtt cacgtgtcct gattgatgct ttcgattggg aagagttacc
    60181 tgatgaagga aagatcttag atgtgggatg cgggtatgga ccgattgggt tagctttggc
    60241 atttgcgaca cagcggtttg ttgaaatggt cgacattaat gaacgagcgg taagtctagc
    60301 gcaaggaaac gcaaagcgta accaaatcga acatgttgat atccatcaat cgaatatcta
    60361 tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc aatccgccga tccgtgctgg
    60421 aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct cggttgaaga agggcggtac
    60481 actgacaatt gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga
    60541 aacatttggt aatgcagaaa tcgtcacaaa agataaagga tattatatca tcaaaagtgt
    60601 gaaagaataa ctagctgaaa aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt
    60661 acgggtacgt atcagttctt tttttgttga gaagttttta aaaacaaaga actatttttt
    60721 cttcaaaaag aaatagtagg aacagagtcg ttgtttttta cgataaaata aataagatac
    60781 agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc aaaaaacata
    60841 ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg gaatttagat
    60901 agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg gcaggcgtat
    60961 cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt gataagacaa
    61021 gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat ttcgcttcaa
    61081 atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc actgatttct
    61141 tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc tatgtgatca
    61201 tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa gagctttccc
    61261 accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag aaatatgcat
    61321 tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc
    61381 gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt
    61441 ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga tactatcaat
    61501 tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg
    61561 ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca tcaaaaaaat
    61621 tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg gcgatcggat
    61681 gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca gtcataggat
    61741 tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg cagcagccaa
    61801 gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa
    61861 gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat
    61921 tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag gctagttagt
    61981 gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct aaaaataatg
    62041 tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc tgattgaaaa
    62101 aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac
    62161 gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt tttatcaaga
    62221 catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg gtgatgtcat
    62281 cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg gcgttggtga
    62341 cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag tggctaaaat
    62401 gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag caattcccgg
    62461 atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc
    62521 ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg ccttgcgtga
    62581 cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga gtaaaaaaat
    62641 cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg ctttcatgaa
    62701 gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga aactggctaa
    62761 ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag ctatcggaaa
    62821 tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag aagatctagt
    62881 agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag ctaagacaac
    62941 cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt
    63001 caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc cagaacgaat
    63061 tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat
    63121 cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga
    63181 agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg acactgtgac
    63241 aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt ctgtcattga
    63301 attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt tgattcatga
    63361 catcatcaca gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga
    63421 aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat ctaaagaagc
    63481 tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct gcatcaatcc
    63541 agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg tatgtacagt
    63601 aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa caacagatgc
    63661 catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa
    63721 ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa aagatcgtgc
    63781 gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca tccgagcttg
    63841 cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg
    63901 cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt
    63961 caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg aagctggtgc
    64021 tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg gtgaaggcta
    64081 ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat caacacagct
    64141 attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg cgcttgtctc
    64201 gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc atttggatta
    64261 acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga aaaaacattt
    64321 acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg tgcatgtcga
    64381 caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg agacaatggt
    64441 gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga aaaagatcta
    64501 taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt ttaggaggct
    64561 ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta gcagcagggt
    64621 tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc aaaagctcat
    64681 cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac cgttcattca
    64741 accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa
    64801 atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca aatattgacc
    64861 aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta aaaccagcga
    64921 tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat gatgtcatcg
    64981 atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct tatcttgcag
    65041 gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag
    65101 gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag
    65161 aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct gctccagata
    65221 aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc ttccatgctt
    65281 caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa tcaggcgaca
    65341 aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa tacacattaa
    65401 acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga acagtagtag
    65461 aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac actgtttatg
    65521 gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag
    65581 cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca gaaactcctg
    65641 aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag gctatggttt
    65701 gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg ataagggaac
    65761 aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg aatcttagtc
    65821 aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca agaaaatatt
    65881 gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa tgacaacatc
    65941 aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa
    66001 tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa aattttgatg
    66061 cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg gattgggatg
    66121 gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat cgtgttggga
    66181 agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa
    66241 gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga tatttcagtt
    66301 ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga tgtactgatt
    66361 tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat cgtgatcatg
    66421 aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt agatgaaatc
    66481 aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg tacagtcaac
    66541 gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac ggtttcattc
    66601 aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga aaatctagtg
    66661 gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt tcgtgctggc
    66721 gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat ccaagctttg
    66781 actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga catcactaat
    66841 aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga ccgtcataaa
    66901 tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac ctatcatcaa
    66961 aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca tgccagagaa
    67021 ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa agctttatca
    67081 ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc tgatctgttg
    67141 attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat tcataaacgt
    67201 ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga attagaagaa
    67261 attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt cggtatcgtt
    67321 gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta taccttagaa
    67381 gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt
    67441 ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag gcgcgatatt
    67501 gatggctgct tttggttttc agcccatcca aggttattct tctatgctag atgcagccct
    67561 tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga tttttacagc
    67621 attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt ctggacaagc
    67681 actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt
    67741 tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg cagcggctgt
    67801 tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa
    67861 ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg aaagctatcg
    67921 ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt taccatggtt
    67981 aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct
    68041 tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc gttctgttgg
    68101 attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga tcgttttgtc
    68161 gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat taggaacgta
    68221 tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa tggctgtttc
    68281 cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt tcagtatttt
    68341 gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt
    68401 atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc
    68461 taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat cgaaaccaac
    68521 gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat tgccttgatc
    68581 cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg cggaactttt
    68641 tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat gggtgctttt
    68701 agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac accatggatc
    68761 gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc agtagcaaca
    68821 gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat ggcacctgct
    68881 ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa catcacgcag
    68941 aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg accaattttc
    69001 tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct agcttgggtg
    69061 atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct
    69121 gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact gatttctggc
    69181 ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa cttttcagct
    69241 ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg taagtggaat
    69301 cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt gagtatcatt
    69361 ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat tgctccatat
    69421 ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc aaaagcaaac
    69481 gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg ggttacggga
    69541 agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag aaagaaggaa
    69601 taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt ggagaagcac
    69661 cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt gcgaaagaag
    69721 caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt cctcttgaag
    69781 gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa gtttctgtag
    69841 ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa actccatttc
    69901 gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc
    69961 gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat tacggtgaac
    70021 accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc cttcagatcg
    70081 cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa ttcgttcgtg
    70141 atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg tacgtgggtg
    70201 aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat ccattcggac
    70261 acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt ggaaagatca
    70321 acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc aatatggatg
    70381 gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc tttaccaatt
    70441 tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat gcgcatgcga
    70501 ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa gacgacttgc
    70561 taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat catacgcgag
    70621 aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag
    70681 gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct tcgactgaaa
    70741 atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa agcaaatgac
    70801 caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag gagtaaaaga
    70861 aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg aaatcgaaga
    70921 tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag tagttgggca
    70981 tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca tgcaagggag
    71041 attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac gtgtgatggc
    71101 cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca acgaatcatt
    71161 tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg aaaatccttt
    71221 gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc aagcttatac
    71281 gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga
    71341 aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc
    71401 tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca tcgtagcagc
    71461 tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag caggtatgca
    71521 agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac aatcttttaa
    71581 agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa atgagtgtgc
    71641 atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga gatcctttgc
    71701 gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat caggtacgcg
    71761 gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa ggaacaggaa
    71821 tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat ggtgtaaaga
    71881 aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag
    71941 tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc caagcctttc
    72001 attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata gctaaagaaa
    72061 agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac aaagatgatt
    72121 tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg gaagcagcgg
    72181 ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg accgttagcg
    72241 accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa
    72301 tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa aaagctgccc
    72361 tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg acttggctgg
    72421 acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt gaatctgccg
    72481 tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt gatgaatatt
    72541 gtgacttgtg accgagaagt tggcaggaga aaaccgttta tacgaaaaaa gggactgtga
    72601 caaatcgtgt cacagtccct tttttatagt gttttttata ggtagtattt tttagcaact
    72661 gttaagtcat catttaattc ataaacaagt ggttgacctg ttggaatttc aagatccatg
    72721 atgtcttcgt cagagatacc ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt
    72781 gcagctacta aaacagtttt gttgtctagt aatgctggag caatttcatc ttgccagaat
    72841 ggcaaagcgc gttctaatgt cactttcaag ttttctccac caggaatatc gcgtttgtct
    72901 aacattgcgt aacgacggtc atttgctgca gaaccttcgt cagtttcttc cattaatgga
    72961 ggaagagtat cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta tttttcagct
    73021 gtttcttttt tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa gcgccatgat
    73081 ttgatttgtg gtacccaaag ttgatctgaa tattctaaga tcaagttaca tgttttgatt
    73141 gcacgagtta aaacagaagt gtaagcaaca tcaaattcga ttccagcttc tttgattttg
    73201 cgtccgcctt cttttgcttc ttcgattcct tctggtgcta gatttacatc agcccaacca
    73261 gtaaattggt tcaatgcatt ccattcacta agaccgtgac gagaaaaaac taattttggc
    73321 atgtttagct ctccttttaa gataggttta tatttctgaa attttcagtt ctgttttatt
    73381 gtacaatttt ttggaagaga tttccatcga aaaattagat aagattctat gagaaaacaa
    73441 gtagagaata taaaaaaacg aacagtgccc acgcaactgt tcgtttttag gtattagatg
    73501 cataattgct cgatttcttt ttcaggtaaa ttgatatatt tataacatgt tccaactgaa
    73561 acaccgcatt ttgaagaaat aaattggatc gtttcttttt tttcataata gagacgtcgg
    73621 atttttttga tcgtttttgc atcgattttt ggtcttccgc cgatttttcc ttttttacga
    73681 gcttcatcta atccaactaa tgtacgttct ttgatcaatg cgcactccat gtttgctagg
    73741 ttttccatta attggaaata gatttttccc atttcgcgcg tatcgattcc ttcatcaaga
    73801 ctaatcaagt gcagcccgcg atctttgaat aattgcgtga gttctgttaa ttgccgcgta
    73861 gatttgccta atcgatgaag ttggcaaatg actagtgcat cgccttgttg catgtcagcg
    73921 atcactgact ccaaatgaac agcagtagaa tcattttgtt ccacttcata tgtctcatgg
    73981 aaaatttctt gacaaccaaa gtcagataat tgatcaacct gagttgttaa atcaacgtca
    74041 tttatggttg tgcgtgcata accaataatt ttcatggtga ttcctttcta gcagtgattt
    74101 tctaactgga tataacagta aaaatgtagc ataaagtacg tcaaaaagat agttttttcg
    74161 caaattttcc gctgtctttt tttattggat gatcaagcag tttttcttta tttacgacgg
    74221 attgcgtcaa gaatctcaac gcctttttct gagccgacaa ctactttaga aaccatgttt
    74281 aggaaaagcc catgttctac taccccaaca agttcatcta aataggtagc taagctttct
    74341 ggctgttcga tgacttctag atgcagatca atgatgtaat gaccaccatc tgtggttaag
    74401 acttctccgt tttcgtctgt acgcaagacg ggttggaatc ttttttcttc aaaaatatga
    74461 agtagctgtt gggagccata agggatcact tcaactggga gtgggaactt cccaagttta
    74521 tcgactaatt tagaagaatc aacgatccaa atcgtttctt ttgagtacgt agcgactatt
    74581 ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt gaaaatcagc actgatttca
    74641 tcagcaccgt ctattgtcaa atcgactacg gggacttcat caatactctt taacggtatt
    74701 cctaattttt ccgcttgttc ttttgtcgca tttgaagtag taactcctgt gatacgtaaa
    74761 ccttcttcac gcatacggcg accaatctct tctaccatat aataggcggt tgaaccggtc
    74821 ccgagaccta cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc agctaattgt
    74881 tttaaattca tctttgcgtc cacctttttt tagtttgagc atcgtgcttt cctcactatt
    74941 ttagcgtgat ttacggtaaa aagataccat tcttttttat tccaataaag ctttttcaaa
    75001 aaaaattgca tctcttgttg acatgttttt tcattcgtga tattctaaca aaggtgcttt
    75061 atctacaggt ttctgtgaag aaacgtgctg actgtttatc aaagcatatt tgataaggat
    75121 gcagacattg catttatttt ttatcacaaa aaaagaacca cctggatgtg tggaactaga
    75181 cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat taaccaatta gtacgtaaac
    75241 ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt gaacaaagga tataacagct
    75301 ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg
    75361 gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt
    75421 caaacttaat tgaagttact gcttatatcc cagggattgg tcataactta caagaacaca
    75481 gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc aggggtacgt taccatatcg
    75541 ttcgcggtgc tttagatact gctggtgtta acgaccgtaa acaaagccgt tctaaatacg
    75601 gaactaaaaa acctaaagct taattttaat tattaaacaa atgaaacttg cttctactat
    75661 atagaagtaa ttactatgaa caatatttcg gaaggaggag ttacggatgc cacgtaaagg
    75721 tcctgttgca aaacgtgatg ttttaccaga tcctatttat aactcaaaat tagtaactcg
    75781 cttgatcaac cgtgtaatgg ttgacggaaa acgcgggatt gcagctaaca ttatctataa
    75841 tgcctttgac ttgatcaaag aatctacagg taacgatcca ttagaagtgt ttgaacaagc
    75901 aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta
    75961 ccaagtacca gttgaagttc gccctgaacg ccgcgttact ctaggtcttc gttgggtagt
    76021 aaactacgct cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa aagaaatcat
    76081 ggatgctgca aacaacacag gtgcttctgt taaaaaacgt gaagacactc ataaaatggc
    76141 tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt tggtcggctt
    76201 ttataagtcg acctcagata gtgtccttat ttttagcaac tagatagtaa agagaggaga
    76261 aacaaggaga atgaccagag aattttcttt acaaaacact cgtaatatcg gtatcatggc
    76321 ccacatcgat gctggtaaaa cgactgctac tgaacgtatc ttgtactata ctggtaaaat
    76381 ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg gactggatgg aacaagaaca
    76441 agaacgtggt attacgatca cttctgctgc tactacagca caatggaagg gtcaccgaat
    76501 caacatcatc gacacaccag gacacgtaga cttcacagtt gaagttgaac gttcattgcg
    76561 tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac ctcaaactga
    76621 aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg caaacaaaat
    76681 ggacaagatc ggtgctgact tcttatattc agtatcaact ttacatgacc gtttgcaagc
    76741 aaacgctcac ccaatccaat taccaattgg tgcggaagac gacttcacag gaattatcga
    76801 ccttgtaaca atgaaggctg aaatgtatac aaacgatctt ggaacagaga tcgaagaaac
    76861 agaaattcct gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc
    76921 tgtagctgaa actgatgaag aacttacttt gaaatactta gaaggtgaag aaatcactga
    76981 agctgagtta aaagaaggta tccgccgcgc aacagttaac gtagaattct acccagtact
    77041 atgtggttca gcatttaaga acaaaggggt tcaattacta ttggacgctg ttcttgatta
    77101 cttgccatca ccgttagata tcccagctat caaagggatt gaccctaaaa ctgatgaaga
    77161 agtagaacgt cctgcagacg acagcgctcc tttctcagct ttagcattta aagttatgac
    77221 tgacccattc gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt tacaatcagg
    77281 ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta tcctacagat
    77341 gcatgccaac tctcgttcag aaatctctga agtttacgct ggagatatcg ctgctgctgt
    77401 tggtttgaaa gacacaacta ctggggacac actatgtgat gaaaagagtc tagttattct
    77461 tgaatccatg gaattcccag aaccagttat ccaagtcgct attgaaccta aatcaaaagc
    77521 tgaccaagat aaaatgggtg tggctttaca aaaactatct gaagaagatc ctactttccg
    77581 tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg aacttcactt
    77641 agatatcatc gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc
    77701 tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta agtttgtacg
    77761 tcagtctggt ggtaaaggtc aatacggtca cgtatggatt gaatttacac caaatgaaga
    77821 aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat
    77881 tccagctgtt gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc
    77941 attagtagat atcaaagcta aactttacga tggttcatac catgatgtcg attcaaatga
    78001 aactgccttc cgtgttgctg cttctatggc attacgtgca gcagctaaga aagctaatcc
    78061 agtaatcctt gaaccaatca tggctgtaga agttgttatc cctgaagatt atttaggtga
    78121 tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg
    78181 cggccaacaa gttgttcgtg cgatggttcc acttgctgaa atgtttggtt atgctacaac
    78241 tcttcgttct gctactcaag gtcgtggtac atttacaatg acatttgacc actacgaaga
    78301 tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac ggcggaaaag ctgaataatt
    78361 tcttaaaacc ggatagtcct gcaaagagat tatccaaaga gactatattt ttaatatagt
    78421 ttcgtgtaaa ataattatcg atgatatggg catgtggtgt accttgtgta catcatatac
    78481 ctatcagaca aataaaaaat atattgtatt tttaggagga cattttaaaa tggcaaaaga
    78541 aaaatttgac cgttctaaac cacacgttaa cattggtact atcggacacg ttgaccatgg
    78601 taaaactaca ttaacagctg caatcacaac tgtactatct aagaaaaacg gcggccaagc
    78661 tatggcttac gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa tcacaatctc
    78721 aacagctcac gttgagtatg aaacagatac acgccactac gctcacgttg actgcccagg
    78781 acacgcggat tacgttaaaa acatgatcac tggtgctgct caaatggacg gagctatctt
    78841 ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg
    78901 tcaagttggt gttccttaca tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga
    78961 agaattacta gaattagttg aaatggaagt tcgtgaccta ttaacagaat acgaattccc
    79021 tggtgacgat gttcctgtag ttgctggatc agctttgaaa gctctagaag gcgacgcttc
    79081 atacgaagaa aaaattcttg aattaatggc tgcagttgac gaatacatcc caactccaga
    79141 acgtgacaac gacaaaccat tcatgatgcc agttgaagac gtgttctcaa ttactggacg
    79201 tggtactgtt gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg acgaagttga
    79261 agttgttggt attgctgaag aaacttcaaa aacaacagtt actggtgttg aaatgttccg
    79321 taaattgtta gactacgctg aagctggaga caacattggt gctttactac gtggtgttgc
    79381 acgtgaagac atccaacgtg gacaagtttt agctaaacca ggtacaatca cacctcatac
    79441 aaaattctct gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc atactccatt
    79501 cttcactaac taccgtccac aattctactt ccgtacaact gacgtaacag gtgttgttga
    79561 attaccagaa ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg aagttgaatt
    79621 aatccaccca atcgctatcg aaaacggtac taaattctca atccgtgaag gcggacgtac
    79681 agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc acttaagttt taaagtaaag
    79741 caacatttat tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg ttccgagctt
    79801 ttttatttat tgcaggatca ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa
    79861 ataaaagagt cagtattttc taaatgaaac tttatgaaaa caaaatgttt tttacaagaa
    79921 aaagccttgc tttgtgagtt tatatatatt ataatgacaa aggtgcgttt actttagtga
    79981 attgcacaga cgtaatcaga atgatttcgt cggctaagaa acgagaggtt gcgacacgcc
    80041 cggacgcttt gccacgaacg agcgtgacgg aaattttcgt ggagctatgt ctacttttca
    80101 aaataggcga aggagggaac aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt
    80161 atgaacaccg tattttagat caatcagcgg ataaaatcgt ggaaactgca aaaagaactg
    80221 gagctagcgt gtctggtcca attccattac caactgaacg cagtctttac acaatcatcc
    80281 gtgcgactca taaatacaaa gattcacgcg aacaattcga aatgcgtaca cacaaacgtc
    80341 ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag ctagacttac
    80401 catctggtgt aaacatcgaa atcaaattat aaaactaaaa caaaaataat ggaggtgtac
    80461 tcatgaccaa aggaatctta gggaaaaaag tgggaatgac acaaatcttc actgaatctg
    80521 gtgaattaat tccagtaact gttgtagaag ctactccaaa cgtagtacta caagtgaaaa
    80581 caatggaaac ggatggctac gaagctattc aagttggtta ccaagatatg cgtgaagttt
    80641 tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct aagcgcttca
    80701 ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt aggaaaagaa attacagtag
    80761 atgttttcca agcaggagac attattgatg taacaggtac tacgaaaggt aaaggattcc
    80821 aaggggttat caaacgccac ggacaaagcc gcggaccaat ggctcacggt tctcgttacc
    80881 atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc
    80941 ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg
    81001 atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa
    81061 ttactatcaa atcagctgtg aaagctaaat aattgtagga agaaaggagg aactaaggaa
    81121 tgccgaatgt agcattattc aaacaagatg gaagccaaaa cggtgaaatc acacttaacg
    81181 aagaaatctt cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac
    81241 gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg
    81301 gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct atccgctcac
    81361 cacaatggcg tggaggtggc gtagtctttg gaccaacacc acgttcttac agctacaaac
    81421 ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa
    81481 acaacttggt agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac
    81541 aagttcttgc taacttatct atcgactcta aagtattggt tgttctagaa aaaggtaacg
    81601 actttgctgc tttatcagca cgtaacttac caaacgtttc tgtagtaact tctgataatg
    81661 tgagtgtgct agacgttgta tcaaatacaa aagtgttagc aacacaaact gctcttactc
    81721 aaattgagga ggtgcttgca taatgaactt actagacgta atcaaacgcc cagtgatcac
    81781 tgaaaaatca atgcttgcca tggatgacaa gaaatacact tttgaagtgg acactcgcgc
    81841 aaacaaaact ttagtaaaac aagcggtaga agctgctttt gacgttaaag tcaaaaacgt
    81901 gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag gatatacaaa
    81961 aaaacgtcgc aaagcgatcg ttacattgac tgaagattca aaagaaatcc aattattcga
    82021 agctgctgaa taagcactaa tagataactc attaaattag gagggaaaaa cgtggcgatt
    82081 aaaaagtaca aacctacctc aaatggtcgt cgtaacatga caggttctga ttttgctgaa
    82141 atcacaacat caacacctga aaaatcattg ttacaaccat tgaaaaacca tgctggacgt
    82201 aacaacaacg gccgcatcac tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc
    82261 gtgatcgact tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac gatcgaatat
    82321 gatccaaacc gttcagctaa catcgcgtta gtacactatg aagatggagt gaaagcttac
    82381 atcttagcac ctaaaggact agaagtgggt atgaaacttg tttcaggtcc agaagcagat
    82441 atcaaagtcg gaaatgctct tccattagaa aacattccag ttggtactgt tatccacaac
    82501 attgaaatga aacctggtaa aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa
    82561 gtacttggta aagaaggcaa atacgtattg atccgcttga actctggtga agttcgtatg
    82621 atcttagctg catgccgtgc tacaatcggt tctgtaggta acgaacaaca cgaattaatc
    82681 aacatcggta aagctggacg ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc
    82741 gtaatgaacc ctaatgatca cccacacggt ggtggtgaag gtaaagctcc tatcggacgt
    82801 aaagcaccag tttctccttg gggtcaacca gctcttggtt acaaaactcg taacaaaaaa
    82861 gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata aataattact ttgaccatgt
    82921 gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg accttttgtc
    82981 gatgagcatt taatgaaaaa agttgaagct caagcagggg ttgaaaagaa aaaagttatc
    83041 aaaacttggt cacgccgttc aacagttttc ccaagttttg ttggatatac catcgcagtt
    83101 tatgatggac gtaaacatgt accagtttac atccaagaag acatggtagg acacaaatta
    83161 ggtgaatttg cacctacaag aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa
    83221 cgctaatttg aggggaggat aaatccaaat ggcagaacaa atcacatcag ctaaagcaac
    83281 tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta gtaattgacc ttatcagagg
    83341 taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat ctgctggaat
    83401 cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa aataactttg acttagatgt
    83461 tgaaaacttg gtagtaagcg aagcttttgt taacgaagga cctacaatga aacgtttccg
    83521 tcctcgtgca aaaggatcag cttctccaat caacaaacgt acaagtcata tcacggtagt
    83581 cgtatcagaa aaataaggag ggataatcag tgggtcaaaa agtacatcca attggaatgc
    83641 gtgtaggcat catccgcgac tgggatgcta aatggtatgc tgaaaaagag tatgctgagt
    83701 tcttacacga agatttaaga atccgtaaat ttatttcaac taaacttgct gatgctgctg
    83761 tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta
    83821 aaccaggtat ggttattggt aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa
    83881 acaaattaac tggtaaaaga atccatatca atatcgtgga aatcaaaaaa ccagatctag
    83941 atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc
    84001 gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc
    84061 aagtatctgg tcgtctaaac ggagcagata tcgctcgttc agaaggatac tcagaaggaa
    84121 ctgttcctct tcatacttta cgtgcggaca ttgattacgc atgggaagaa gcagatacaa
    84181 catacggaaa actaggagtt aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa
    84241 aaaacactga gaaaggaggg aaataatcat gttagtacct aaacgtgtaa aacaccgtcg
    84301 tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag cattcggtga
    84361 atacggtttg caagctgttg attcacattg gatcacaaac cgccaaatcg aagctgctcg
    84421 tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta tggattaaaa ttttccctca
    84481 caaatcttat actgccaaag caattggggt acgtatgggt tctggtaaag gggcacctga
    84541 aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga
    84601 agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga aaactaagat
    84661 cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc agagaattaa
    84721 ccactgccga aatgcttgat caagaaaaac aattaaaaga agaattgttt aatcttagat
    84781 tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga
    84841 ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta attatggaag gaggccatta
    84901 agcgtatgac tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg gtttcagaca
    84961 aaatggataa aacaattacc gttgctgtcg aaacaaagaa aaaccaccct atctatggca
    85021 aacgcatgaa ctattctaag aaatacaaag tgcatgatga aaacaacaca gcaaaagttg
    85081 gcgacatcgt gagaattatg gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt
    85141 tggaagtagt cgaagaagca gttattatct aataacacga gattttccta tatagattga
    85201 atgcaaaatc tgaaaggagg atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg
    85261 ctgataactc aggcgcacgt gaaattttga cgatcaaagt ccttggtgga tctggtcgta
    85321 aaactgctaa catcggtgat gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg
    85381 ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc
    85441 gtacagacgg ttcatatatt aaatttgatg aaaatgctgc agtgattatt cgtgatgata
    85501 aaagccctcg tggaacacgt atctttgggc cagttgcacg tgaactacgt gaaaacaact
    85561 tcatgaagat cgtttcacta gcaccagaag ttttataatc ttgagacact tatcaaagga
    85621 ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt taaagttatc actggaaaag
    85681 acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa aaaagataaa gttattgtcg
    85741 aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca agctgctcct caaggtggaa
    85801 ttgttgagat ggaagcgcca attcatgttt ctaacgtgat ggtggttgac tctactggtg
    85861 tagctggccg agttggctac aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa
    85921 ccggtgaagt tttagataaa taaaagttta aggaaggagg gtcttactga atgaaccgcc
    85981 taaaagaaaa atatcttaaa gaagtaactc catcattgat ggaaaaattt aactatagct
    86041 ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg
    86101 tatcaaacgc taaaaactta gataaagcag ttgaagaatt aactttgatc actggtcaaa
    86161 aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc
    86221 caattggtgc aaaagttaca ttgcgcggag aaagaatgta cgaattctta gacaaattgg
    86281 tttctgtttc actaccacgt gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg
    86341 gtcgtggaaa ctatacttta ggtattaaag aacaattgat cttcccagaa gtagattatg
    86401 atttagtaga taaagtacgt ggtatggaca tcgttattgt aacaacagca aactctgatg
    86461 aagaatctcg tgagttgttg acacaattag gcatgccatt ccaaaaataa ttaaaggagg
    86521 cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa
    86581 cacaagctta tactcgttgc gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc
    86641 atctttgccg tatttgcttc cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga
    86701 aagctagctg gtaaaaagta aattcgcata agaaggaggt aaaagatcaa tggtcatgac
    86761 agatccaatt gcagattttc taactcgtat ccgtaacgca aacatggtta aacatgagtc
    86821 tttagaagta cctgcatcaa aaattaaacg tgacatcgct gaaatcttga aacgtgaagg
    86881 tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct
    86941 aaaatttggt aaaaacggcg aacgtgttat cacaaactta aaacgtatct ctaaaccagg
    87001 tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt taggtatcgc
    87061 aatcatttcg acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg
    87121 cggcgaagtt atcgcttacg tatggtaatc aagtagaatc tacaaggagg tgtctttaag
    87181 tgagccgtat cggaaataaa gtcgttgtta tccctgaagg agtaacagtt actcaagacg
    87241 gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac tcgtactttt tcagcagata
    87301 tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa
    87361 tgaaaacaat tcatggaaca actcgtgcga acttcaacaa catggttgta ggtgtcagcg
    87421 aaggtttcca aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa ttacaaggat
    87481 caaaacttgt attgaacgtt ggttactcac atccagtaga aatcacacca ccagctggag
    87541 taactgttga agtaccagct aacacacaag taattgttaa aggtgcgaac aaagaagaag
    87601 ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag
    87661 gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt aaataatcgt
    87721 ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca tttggctgaa gtttaccttc
    87781 aaaaaaatac atttttactt gtgccgcagc ttctattttt cgaaacgtat ttactgcttg
    87841 aaatagtagt acaataaaaa taaagaggtg acaattgtga ttacaaaacc agataagaac
    87901 aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc
    87961 ccacgcttga acgtttttcg ttctaacaaa aacatctacg ctcaaattat tgatgacgta
    88021 gcgggtgtaa cgctagcaag tgcctctacc ttggataaag aaatttcagg tggaacaaaa
    88081 acagaagcag cacaagctgt cggtaaacta gttgctgaac gtgcagctga aaaaggcatt
    88141 aaagtagtag tctttgaccg tggtggatac ctttaccatg gccgtgtgca agctttagct
    88201 gaagctgctc gcgaaaatgg actagaattt tagaagaagg aggaatacca ttcatggttt
    88261 atatcgatcc aaaacatttg gaattagaag accgcgttgt tgcaattaac cgtgtaacaa
    88321 aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa
    88381 acggacacgt agggtttggt acaggtaaag ctcaagaagt tcctgaagca atccgtaaag
    88441 caattgaaga tgcgaagaaa aacttagttg aagtacctat ggttggttca acaatcccac
    88501 acgaagtaat cggtgctttt agtggtggta gaatcctaat gaaacctgct gtagaaggtt
    88561 ctggggttgc tgctggtgga ccagttcgtg ccgtattaga attagctggg gtagcagata
    88621 tcacttctaa atctttaggc tcaaatacac caatcaacgt tgttcgcgca acagttgaag
    88681 gtttgaaaca attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag
    88741 aattaatcgg ataaggagga caacaataat ggctgaatta aaagttactt taaaacgcag
    88801 tattatcgga cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc ttacaaagat
    88861 taacagttca gtggttaagc cttctaatga agcaattaaa ggcatgatca atactgtttc
    88921 tcacctagtg gacgttgaag aaatttaatt tagaaaaatc ataactagaa cattaaggag
    88981 gtgccaaacc tatgaaactt catgaattaa aacctgctga aggttcacgc caagtacgta
    89041 accgtgttgg tcgtggtact tcatctggta acggaaaaac tgccggacgc ggacaaaaag
    89101 gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga caaacaccat
    89161 tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac tatgcagtca
    89221 ttaatcttga tgtattgaac cgctttgaag acggagctga agtaactcct gttgctttag
    89281 ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct aatggggaac
    89341 taaacaagaa attaactgtg aaagcagcaa aattctctaa agcagctcaa gaagcaattg
    89401 aagcggctgg tggatcaatc gaggtgatct aatgtttaga ctattaaaag atgcttttaa
    89461 agtaaaagac attagatcga aaatcctatt tactgtattt gtgttgtttg tatttcgcct
    89521 aggtgcacat attactgtac caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt
    89581 gccatttttc aacatgttga acttggtaag tggtagtgcg atgcaaaact tttccatttt
    89641 ctcaatgggg gtttcaccat acattactgc ttctatcgtc attcagcttc ttcaaatgga
    89701 catcgtgcct cgatttgttg aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa
    89761 tcaagcgact cgatatctga cattagtatt ggcatttatc caatctgtcg gtatcactgc
    89821 tggattcaat caatacgctc aattaggttt cgtgaataat cctaacgtag caacttacgt
    89881 aacgatcggt ttgattctaa cagccggtac gatgtttgtt acttggctag gagaacagat
    89941 cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt tttgcaggta tcatttctag
    90001 attgcctgaa agtatcagag aaatttatga agattacttt ataaatattg atccttcaga
    90061 catatggaaa tctgctattt ttgtggccat tttactagta gcggtcttgt tgatcgtgac
    90121 attcgtaaca tttttccaac aagctgaacg caagatcccg atccaatata cgaaacgcgt
    90181 agctggcgcg cctacaagca gctatctgcc gttgaaagta aatgcagctg gagttattcc
    90241 agttatcttt gctagttcgt ttattgctac gccaaatgct gttttacaag cattgagcgg
    90301 tagattcggt tctgaaaact ggtacaaagt gatgatggag atctttaact ataatacagc
    90361 acctggtgct acgatttata cgattctgat cgttgccttc actttcttct acgcctttgt
    90421 tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt atataccaag
    90481 cgtacgccct ggtaagggga ctgaagagta tgtctcaaga ttactgatgc gtttaagtac
    90541 agttggtgca gtcttccttg gtttagtggc attgcttcca atcattgctc aaatggtttg
    90601 ggatcttcca caatcaatcg gattaggcgg tacaagctta ctgatcgtta tcggtgtagc
    90661 tttagaaact gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg ttggattcat
    90721 tgagtaagaa agcgtgttga ggtttcccct caacacacgt tctcacacat ttattaggag
    90781 ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc aggtaaagga acacaagctg
    90841 aaaaaatcat tgatgcttat ggtattccgc acatctcaac aggagatatg ttccgcgcag
    90901 cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc ttatatggat aaaggtgcat
    90961 tagttcctga tgaagtaaca aatggaatcg tgaaagagcg actagctgaa ccagatacag
    91021 agaaaggctt cttattagat ggctttccac gtacgctaga tcaagcaaaa gctctggacg
    91081 caatgctaaa agatttgaat aaaaaaattg atgctgtcat cgatatccat gttggtgaag
    91141 aaatcttggt cgaacgtcta gccggccgat ttatctgcag caattgtgga gcaacttacc
    91201 ataaaatttt caaccctaca aaagttgaag acacatgtga tcgttgcggt gggcatgaat
    91261 tctatcaaag agaagatgat aaacctgaga cggttaaaaa tcgtctggct atcaacatca
    91321 aaaatagtga accaatcttg gcttattata aagaacaagg tttgctaaac actattgatg
    91381 gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa aatcattgaa aaatagcacg
    91441 attgcccgta gccgacttgc attatgcagt tactcttgtc aagttcaata aattatgata
    91501 gaatatgtca gtccgacttc tagagaatcc tctagatgat ccattttgag aattttgagg
    91561 tgggaactgc agtttccgcc attttatcgt gtgaaaatgc gaaacaagta caaggaggta
    91621 ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga aactttgccg
    91681 aatgcaatgt ttaaagttga actagaaaac ggacaccaag ttcttgctac tgtttcaggt
    91741 aaaattcgta tgcattacat ccgtatctta cctggtgaca aagtaacggt tgaattgtct
    91801 ccatacgatc ttaaccgtgg ccgtattact tatcgcttta aataattgta ctccgtaaaa
    91861 ctacaggagg tattatcatg aaagtaagac catcagtaaa accaatgtgc gaacattgta
    91921 aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca aaacataaac
    91981 aacgtcaagg ataattggag gtgctataaa tatggctcgt attgcaggag tagatattcc
    92041 tcgtgataaa cgtgtagtag tttcacttac ttatatctat ggtattggta acactactgc
    92101 taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa
    92161 tgaacaaaca gatgctatcc gtgcagaagt agataaatta aaagttgaag gggatcttcg
    92221 tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc ggttcttacc gtggaatccg
    92281 tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa
    92341 aggcccagct cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa
    92401 cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg
    92461 tattgcacat atccattcta cattcaataa cactatcatc atgatcactg atactcacgg
    92521 aaatgcatta gcgtggtcat cagctggttc attaggtttc aaaggaagca aaaaatcaac
    92581 tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg aacacggatt
    92641 aaagactgtt gaagtaacag ttaaaggacc tggttctgga cgtgaagcag caattcgttc
    92701 attacaagct acaggtttag aagtgactgc aattcgtgac gtgactccag ttcctcataa
    92761 tggtgcccgc cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat
    92821 cagtaacgtc attgaacaag acacttttcg ttttgaaagg ggtaaagata agaatgattg
    92881 agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa agattatggc aagttcatcg
    92941 ttgaacctct tgaaagaggg tatgggacta ctttagggaa ttccctacgt cgtattttat
    93001 tatcttcttt gccaggtgct gccatcacta atattcaaat cgatggtgta ctgcatgaat
    93061 tctctaccat caaaggtgtt agagaagacg tcactcaaat catcttgaac attaaaggct
    93121 tagcgctgaa aatgtatggc caagaagaaa aaacccttga aatcgatatt accggacctg
    93181 ctacagtaac tgctggcgat attatcgttg atagtgatgt agaaattctt aacaaagata
    93241 tgtacatctg tagtgtctct gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg
    93301 gacgtggtta tgttcaagca gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc
    93361 cagtcgattc aatctacaca cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg
    93421 ttggacaccg tgaagatttt gataaattga cgatggagat ttggacagat ggttcaattg
    93481 aaccgttgga agcaatgagt ttgtctgcga aaatcatgac tgaacatttg gatatttttg
    93541 tgaacttaac tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa
    93601 aagaaaaaat gttggaaatg acgatcgaag agttagactt gtctgttcgc tcttacaatt
    93661 gtttgaaacg tgcaggaatc aatactgtac aagaacttac aaataaatct gagcctgaaa
    93721 tgatcaaagt ccgtaactta ggacgtaaat cattagaaga agttaaagca aaattgcacg
    93781 acttaggttt aggtttacgt aaagaagatt aaaacctttt acgaaggagg gaaaccaacg
    93841 tgagttatcg taaactagga cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa
    93901 caactgattt attaatcaac gaacgtattg tgactactga agctcgtgcg aaagaagttc
    93961 gctcaactgc agaaaaaatg atcactttag gtaaacgcgg agatttgcat gcacgtcgtc
    94021 aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg
    94081 tcattgaatc agctttacaa aaactgttca acgatattgc acctcgttac gcagaacgtc
    94141 aaggcggata cacacgcatt ttgaagacag aaccaagacg cggtgatggt gcaccaatgg
    94201 ttgtcttaga acttgtctaa aatcacacta ataacatcac tttatagatg attcttttag
    94261 agtgttatga tgttggaaga tatttttccg agtctagctc agcgctgctc ctcaagcgaa
    94321 tatcgcttgg ggagtaagca ctcatcataa agtgtttttt tataccttca attacctgtc
    94381 atgacagata tgtttgaagg cataaaaaaa cattttaagc gagaaataat tgaaaatttt
    94441 gtcttttttt gatagtgtta gtaggaactt taggatgaag aaggagtaat catggagcca
    94501 attattgaat tagaaaagat aaattataaa taccagccag atgatcttcg ccccgcattg
    94561 aaagatgtct cctttacaat tgataaaggt gagtggatcg ctattattgg acacaatggt
    94621 tcaggaaaat cgacacttgc caaaacgatc aatggattgc ttctgccaga atcagggatc
    94681 gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt ggacgatccg acagatggtt
    94741 ggaatggttt ttcaaaatcc agacaatcag tttgttggtt cgacagtaga agatgacgtt
    94801 gcttttggtc ttgaaaatca agggattccc agagaagaaa tgctagtgcg tgtgaaagat
    94861 gcacttgaga aagttagaat ggctgagttt gcttcacgcg agcctgctcg tttatcagga
    94921 gggcaaaaac aacgtgttgc cattgccggc gtggttgcct tacgacctga tatcattatt
    94981 ttagatgaag caaccagtat gctagatcca gaaggtcgtg aagaagtgat ctctacgatc
    95041 aagaagatca aagaagaaag tcaattgacc gttatatcta tcactcatga tattgatgaa
    95101 gcagctaatg ccaatcggat actagtgatg agacaaggcg aacttgtccg tgaaggtaca
    95161 ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact taggtctaga tctaccattt
    95221 cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg atgtgcccag tgaatatatg
    95281 acagaagaaa ggatggtgga ttggttatgg acatccgttt tgaacaagta gacttcactt
    95341 atcagccgaa tacaccattt gaacaaagag ccttatttga catcaatatg acgatcaaag
    95401 aaaacagtta tactgcatta gtagggcata ccggaagcgg gaaatcaacc ttacttcagc
    95461 atttgaatgc gcttgttaag ccaacaagcg gtacagtcca tattggagaa cgagatattc
    95521 agccggatac ggataataag aatttaaagc ccatccgaaa aaaagttggc atcgtctttc
    95581 agtttccaga agcacagcta ttcgaagaaa cggtagcaaa agacattgct ttcggtccta
    95641 aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca ttagaacaag
    95701 ttgggctgga tgaaagctat ttggaacgtt cgccatttga actttcagga gggcaaatgc
    95761 gtagagtagc aatcgctggt gtgcttgcca tgaggccgga agtacttgta ttggatgaac
    95821 caacggcagg acttgatccc cagggtagaa aagaaatgat ggagatgttt tggcgtcttc
    95881 ataaggaaca gcagatcact attgtattag ttacgcattt aatggatgat gtagccaatt
    95941 ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg gagccgcaag
    96001 aggtctttca aaacattgaa tggctaaaag aaaaacaact aggcgtacct actgccaccg
    96061 agtttgcaga agaattaatg gctaaaggga tgaactttgc taccttgcct ttgacagctg
    96121 aagaattagc agacgctatc gtccattatg ctggaggaaa tgcccatgat gaataaattg
    96181 atatttggtc gttatatgcc tggagattcc ttgatccatc gattggatcc ccgagctaaa
    96241 ttattagcca gtttttattt tatcggaatc atttttttag caaataattg ggaaacgtac
    96301 cttttgttgg catttttcac attactttct gtcttcttgt caaaagtaga tttgaaattt
    96361 tttatccgag gcattcgccc attgatctgg ctaatcctct tcactgtagc tttgcaggtc
    96421 ttgtttacaa gtggcggaga aacttattgg agttggggtg ttttccatat cactgaattt
    96481 ggtgtgatga acggcctctt tattttctgt cgattcgtgt tgatcatttt tatgtcgact
    96541 ctactcacgc ttactacacc gcctttggaa ttatcggatg cgattgagta cttgcttcgt
    96601 ccattgaaag ccgtacggtt cccagtccac gaaatttcat tgatgctgtc gattgctctt
    96661 cgttttgtgc ctacattgat ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc
    96721 ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga aagcagtcat tcctttgctg
    96781 attcctttat ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc tatggaagca
    96841 agaggctaca aaggtgggga aggaagaacg aaatatcgtg tattgcattg gcataataga
    96901 gacacgatgg ttatcattgc ttttgtatta ttaactgtgc tactgatctt tttgagagga
    96961 taaacgttca gtgggccgct atctattctt ttcttgctga tctgtttgca agaaaggatg
    97021 cgagcagtgt atgacaagaa atattagggt ctgtggcaga ctaaggtcac aggcctcttt
    97081 cttttatctg aaaagaaaga acgaaaaaat tttataaaaa aatcattatt ccagataacc
    97141 ttttaggaac aaaggagaga aaaacatggt acgttacaaa gcaatcattg catacgacgg
    97201 aacaaatttt aatggattcc aaaaacagcc aaatgggcgt actgttcaag aagaagtaga
    97261 aaaaacatta caaaaaatgg caaatggaaa agaaatcacg gtctttggtt cgggaaggac
    97321 agatgccgga gtacatgcga ttgggcaagt aatccatttt gattatccag aggagcgtcc
    97381 gttagaacga atgcgtttcg cgctagatac ccagtcacca gaggatattg ctgtaagaca
    97441 agtagaaatt gtttcagaag acttccacgc aagatatttg gtcaaagaaa agacgtatca
    97501 atttcgagtg gatataggga aaccacgcag tccttttcga cgccattatg caagttattt
    97561 tccttaccct cttgatttgg aaaaaattca gcgggcgctt cctgatttat tagggactca
    97621 cgatttcacc tctttttgtg catcaggaag ctcgatagag gataaagtac gtacgatcta
    97681 cgaggctaag atggaggtaa atgaaacaaa agacgaattg ttgtttacat ttaggggaaa
    97741 tggcttcttg tataagatga tacggatctt agtaggaacg cttttgaaaa ttggcaatgg
    97801 ccgattgcct gaagatagta tcccagatat tattgcgaaa aaagatcgta atgcagcagg
    97861 acctacagca catcctgaag gactttattt gtatgaagtg aaatatgaat aagaggttgt
    97921 gacagaagtg gtcagcttca agaaataaga aggaatttac gaaaattact tctcaaaatt
    97981 ttgcaaaatt tcggcttatt ttcgaaggag ctacttctgg aacaccgtga atcagaggtt
    98041 gtgaaaaagc tgccctgcat caagtaataa gaacaaccaa acaaaaatag ctcgtcatat
    98101 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    98161 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacggaaaa atagttcctc
    98221 atattttgcc gtttgttgag ctaataccga aggtcagctg attgaacacc gtttaagaaa
    98281 gaggtcgtga gcctgtcatt taactctgag tcacaagtaa caatttcaaa aaacagcttc
    98341 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    98401 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttaatgag
    98461 gacagtcgga acaggtttgt catttttata tttaaatggc acaaattttt ctaattattt
    98521 gtcatttagc gctgaaaatt tttgctattt atctaaaaaa gtgataacta cttaagatga
    98581 agcagaatat ttacgccata cggcatacaa ccgcgaaaaa tataaaaaaa agaaaacgat
    98641 tgaacgcgtt tttgcagtcg caaaggaaaa gcatggaatg cgctacatca agtatcgaga
    98701 cttggaaaac aatcaggtgt atacgctgat tgtttttgct gcaatgaatc tgaaaaagtt
    98761 gtccacttgg cagttggaac atatgtcttc atatctttta ttatacaaaa atagcaaaag
    98821 caccagtcta cactcttagt aaagaggaaa ctgatccttt tgtctacatt cttaaagtac
    98881 ttaaactaag tactttattt tactctttag gattgataca gtagcatcta tccctttatt
    98941 aataatcttt tattttttta aataaagatg ttgtctccat cttcaggcac taaaactttt
    99001 tcggaaattc ctttttcttt attgaaagac tttaattctt ctcttgacag tccccaatgg
    99061 tttacagctt ccatatgaac ggcaataatt gtggaatctg gtattgcttt atgcaattca
    99121 tataaatcgt ctttccccat aataagagat tctccaacta aaaaatgatt atctccagca
    99181 tttgctacta tgatttctgg attatactga tcaatagttt gtttaacagc ttcataccaa
    99241 actgtatctc ctgtaaagta tagtgtcttc tcagtcgaat gtttgaaaac aataccgcaa
    99301 acattaccta cataattcaa gatttctcct ctgccatgtt tgccttttgt tttaaccaat
    99361 tgtatagatt caaaaactgt gtcttctcgt agtacctcca catttttaaa accatatagt
    99421 cgaatttcat tcgcatcatt ttcatcttgc acaaataatt ttatttcttt aggtaggacc
    99481 tctttcgcta tatcgtccca atgatctaga tgtaaatgtg ttacaataac agcatcaata
    99541 ccgttgataa tatcttcgac tggcattggc aaatcaacta gaggattcat ttgatctttt
    99601 cttggtgatg gaaaaggacc tttcgaaata aatggtggaa gtgttccttt ttccgctaac
    99661 attggatcaa ttaaaaattt tttacctcca tagtctaaga tagctgttgc attacgaatg
    99721 tgatgtatat tcataaacgt tgtctccttt ttgttgatat gaaatatttt tagtataatt
    99781 caattccata caataaataa accgaacaaa aaaagtcttt tactattttt acttaattta
    99841 tgaaaggtt
//
LOCUS       DACDHL010000002        77101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDHL010000002 DACDHL010000000
VERSION     DACDHL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.002"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1160)
                     /locus_tag="IV228_000107"
     CDS             complement(<1..1160)
                     /locus_tag="IV228_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8716062.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDT"
     gene            complement(1173..1748)
                     /locus_tag="IV228_000108"
     CDS             complement(1173..1748)
                     /locus_tag="IV228_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP8716063.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(1714..2901)
                     /locus_tag="IV228_000109"
     CDS             complement(1714..2901)
                     /locus_tag="IV228_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8716064.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(2965..4692)
                     /locus_tag="IV228_000110"
     CDS             complement(2965..4692)
                     /locus_tag="IV228_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8716065.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(4689..5141)
                     /locus_tag="IV228_000111"
     CDS             complement(4689..5141)
                     /locus_tag="IV228_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP8716066.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(5253..5633)
                     /locus_tag="IV228_000112"
     CDS             complement(5253..5633)
                     /locus_tag="IV228_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8716067.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(5630..6016)
                     /locus_tag="IV228_000113"
     CDS             complement(5630..6016)
                     /locus_tag="IV228_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716068.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(6018..6296)
                     /locus_tag="IV228_000114"
     CDS             complement(6018..6296)
                     /locus_tag="IV228_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716069.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            6344..7072
                     /locus_tag="IV228_000115"
     CDS             6344..7072
                     /locus_tag="IV228_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP8716070.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(7218..7694)
                     /locus_tag="IV228_000116"
     CDS             complement(7218..7694)
                     /locus_tag="IV228_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP8716071.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(7654..8001)
                     /locus_tag="IV228_000117"
     CDS             complement(7654..8001)
                     /locus_tag="IV228_000117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716072.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(7988..8173)
                     /locus_tag="IV228_000118"
     CDS             complement(7988..8173)
                     /locus_tag="IV228_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716073.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(8174..8446)
                     /locus_tag="IV228_000119"
     CDS             complement(8174..8446)
                     /locus_tag="IV228_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP8716074.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(8443..8646)
                     /locus_tag="IV228_000120"
     CDS             complement(8443..8646)
                     /locus_tag="IV228_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716075.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            8771..8983
                     /locus_tag="IV228_000121"
     CDS             8771..8983
                     /locus_tag="IV228_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716076.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(9016..9210)
                     /locus_tag="IV228_000122"
     CDS             complement(9016..9210)
                     /locus_tag="IV228_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716077.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(9223..9405)
                     /locus_tag="IV228_000123"
     CDS             complement(9223..9405)
                     /locus_tag="IV228_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716078.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(10032..10397)
                     /locus_tag="IV228_000124"
     CDS             complement(10032..10397)
                     /locus_tag="IV228_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716079.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(10437..10739)
                     /locus_tag="IV228_000125"
     CDS             complement(10437..10739)
                     /locus_tag="IV228_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716080.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(10739..11044)
                     /locus_tag="IV228_000126"
     CDS             complement(10739..11044)
                     /locus_tag="IV228_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716081.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(11041..11202)
                     /locus_tag="IV228_000127"
     CDS             complement(11041..11202)
                     /locus_tag="IV228_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8716082.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(11199..11501)
                     /locus_tag="IV228_000128"
     CDS             complement(11199..11501)
                     /locus_tag="IV228_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716083.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(11663..11932)
                     /locus_tag="IV228_000129"
     CDS             complement(11663..11932)
                     /locus_tag="IV228_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716084.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(11944..12795)
                     /locus_tag="IV228_000130"
     CDS             complement(11944..12795)
                     /locus_tag="IV228_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8716085.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            complement(12802..13488)
                     /locus_tag="IV228_000131"
     CDS             complement(12802..13488)
                     /locus_tag="IV228_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716086.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(13494..14165)
                     /locus_tag="IV228_000132"
     CDS             complement(13494..14165)
                     /locus_tag="IV228_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8716087.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(14158..14499)
                     /locus_tag="IV228_000133"
     CDS             complement(14158..14499)
                     /locus_tag="IV228_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716088.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(14677..15375)
                     /locus_tag="IV228_000134"
     CDS             complement(14677..15375)
                     /locus_tag="IV228_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8716089.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNIKVMEVLGGK
                     ESRAYEKISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDTVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            15430..16095
                     /locus_tag="IV228_000135"
     CDS             15430..16095
                     /locus_tag="IV228_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716090.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDHLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            complement(16350..16688)
                     /locus_tag="IV228_000136"
     CDS             complement(16350..16688)
                     /locus_tag="IV228_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8716091.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(16704..16907)
                     /locus_tag="IV228_000137"
     CDS             complement(16704..16907)
                     /locus_tag="IV228_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8716092.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(16922..17668)
                     /locus_tag="IV228_000138"
     CDS             complement(16922..17668)
                     /locus_tag="IV228_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8716093.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            17749..17928
                     /locus_tag="IV228_000139"
     CDS             17749..17928
                     /locus_tag="IV228_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716094.1"
                     /translation="MNKKTDEIAADLVVAWLNHESQVSTKGESISPKEIAQAYLDIHY
                     AVIYGQLPEDRKNDD"
     gene            18359..18679
                     /locus_tag="IV228_000140"
     CDS             18359..18679
                     /locus_tag="IV228_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8716095.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            18697..19128
                     /locus_tag="IV228_000141"
     CDS             18697..19128
                     /locus_tag="IV228_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8716096.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            19255..19839
                     /locus_tag="IV228_000142"
     CDS             19255..19839
                     /locus_tag="IV228_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8716097.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            19953..21092
                     /locus_tag="IV228_000143"
     CDS             19953..21092
                     /locus_tag="IV228_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8716098.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(21161..21234)
                     /locus_tag="IV228_000144"
     tRNA            complement(21161..21234)
                     /locus_tag="IV228_000144"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21198..21200),aa:Arg,seq:cct)
     gene            complement(21623..22803)
                     /locus_tag="IV228_000145"
                     /pseudo
     CDS             complement(21623..22803)
                     /locus_tag="IV228_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(22926..23339)
                     /locus_tag="IV228_000146"
     CDS             complement(22926..23339)
                     /locus_tag="IV228_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8716099.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            complement(23339..23521)
                     /locus_tag="IV228_000147"
     CDS             complement(23339..23521)
                     /locus_tag="IV228_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8716100.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            complement(23552..23773)
                     /locus_tag="IV228_000148"
     CDS             complement(23552..23773)
                     /locus_tag="IV228_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716101.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            complement(23841..25301)
                     /locus_tag="IV228_000149"
     CDS             complement(23841..25301)
                     /locus_tag="IV228_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8716102.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            complement(25409..25729)
                     /locus_tag="IV228_000150"
     CDS             complement(25409..25729)
                     /locus_tag="IV228_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8716103.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            complement(25759..26076)
                     /locus_tag="IV228_000151"
     CDS             complement(25759..26076)
                     /locus_tag="IV228_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8716104.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            complement(26228..28930)
                     /locus_tag="IV228_000152"
     CDS             complement(26228..28930)
                     /locus_tag="IV228_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8716105.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            complement(28993..30270)
                     /locus_tag="IV228_000153"
     CDS             complement(28993..30270)
                     /locus_tag="IV228_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8716106.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            complement(30270..31283)
                     /locus_tag="IV228_000154"
     CDS             complement(30270..31283)
                     /locus_tag="IV228_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8716107.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            complement(31299..32027)
                     /gene="sfsA"
                     /locus_tag="IV228_000155"
     CDS             complement(31299..32027)
                     /gene="sfsA"
                     /locus_tag="IV228_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8716108.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32204..32533
                     /locus_tag="IV228_000156"
     CDS             32204..32533
                     /locus_tag="IV228_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8716109.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            32717..33364
                     /locus_tag="IV228_000157"
     CDS             32717..33364
                     /locus_tag="IV228_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8716110.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            33379..34905
                     /locus_tag="IV228_000158"
     CDS             33379..34905
                     /locus_tag="IV228_000158"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8716111.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(34954..37752)
                     /gene="ileS"
                     /locus_tag="IV228_000159"
     CDS             complement(34954..37752)
                     /gene="ileS"
                     /locus_tag="IV228_000159"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8716112.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(38014..38721)
                     /locus_tag="IV228_000160"
     CDS             complement(38014..38721)
                     /locus_tag="IV228_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8716113.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(38742..39524)
                     /locus_tag="IV228_000161"
     CDS             complement(38742..39524)
                     /locus_tag="IV228_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8716114.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(39573..39860)
                     /locus_tag="IV228_000162"
     CDS             complement(39573..39860)
                     /locus_tag="IV228_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8716115.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(39875..40474)
                     /locus_tag="IV228_000163"
     CDS             complement(39875..40474)
                     /locus_tag="IV228_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8716116.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(40488..41165)
                     /locus_tag="IV228_000164"
     CDS             complement(40488..41165)
                     /locus_tag="IV228_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8716117.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(41182..42423)
                     /gene="ftsZ"
                     /locus_tag="IV228_000165"
     CDS             complement(41182..42423)
                     /gene="ftsZ"
                     /locus_tag="IV228_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8716118.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(42446..43771)
                     /gene="ftsA"
                     /locus_tag="IV228_000166"
     CDS             complement(42446..43771)
                     /gene="ftsA"
                     /locus_tag="IV228_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8716119.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(43927..45147)
                     /locus_tag="IV228_000167"
     CDS             complement(43927..45147)
                     /locus_tag="IV228_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8716120.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(45162..46250)
                     /gene="murG"
                     /locus_tag="IV228_000168"
     CDS             complement(45162..46250)
                     /gene="murG"
                     /locus_tag="IV228_000168"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8716121.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(46276..47637)
                     /locus_tag="IV228_000169"
     CDS             complement(46276..47637)
                     /locus_tag="IV228_000169"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8716122.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(47651..48613)
                     /locus_tag="IV228_000170"
     CDS             complement(47651..48613)
                     /locus_tag="IV228_000170"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8716123.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(48639..50831)
                     /locus_tag="IV228_000171"
     CDS             complement(48639..50831)
                     /locus_tag="IV228_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8716124.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(50832..51233)
                     /gene="ftsL"
                     /locus_tag="IV228_000172"
     CDS             complement(50832..51233)
                     /gene="ftsL"
                     /locus_tag="IV228_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8716125.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(51238..52197)
                     /gene="rsmH"
                     /locus_tag="IV228_000173"
     CDS             complement(51238..52197)
                     /gene="rsmH"
                     /locus_tag="IV228_000173"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8716126.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(52218..52649)
                     /gene="mraZ"
                     /locus_tag="IV228_000174"
     CDS             complement(52218..52649)
                     /gene="mraZ"
                     /locus_tag="IV228_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8716127.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(52819..53187)
                     /locus_tag="IV228_000175"
     CDS             complement(52819..53187)
                     /locus_tag="IV228_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8716128.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53358..54314
                     /locus_tag="IV228_000176"
     CDS             53358..54314
                     /locus_tag="IV228_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8716129.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            54387..54503
                     /locus_tag="IV228_000177"
     CDS             54387..54503
                     /locus_tag="IV228_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8716130.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(54558..56240)
                     /gene="recN"
                     /locus_tag="IV228_000178"
     CDS             complement(54558..56240)
                     /gene="recN"
                     /locus_tag="IV228_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8716131.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(56258..56707)
                     /locus_tag="IV228_000179"
     CDS             complement(56258..56707)
                     /locus_tag="IV228_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8716132.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(56814..57632)
                     /locus_tag="IV228_000180"
     CDS             complement(56814..57632)
                     /locus_tag="IV228_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8716133.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(57641..58531)
                     /locus_tag="IV228_000181"
     CDS             complement(57641..58531)
                     /locus_tag="IV228_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8716134.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(58533..58757)
                     /locus_tag="IV228_000182"
     CDS             complement(58533..58757)
                     /locus_tag="IV228_000182"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8716135.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(58762..60099)
                     /locus_tag="IV228_000183"
     CDS             complement(58762..60099)
                     /locus_tag="IV228_000183"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8716136.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(60100..60954)
                     /locus_tag="IV228_000184"
     CDS             complement(60100..60954)
                     /locus_tag="IV228_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8716137.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(61051..61500)
                     /gene="nusB"
                     /locus_tag="IV228_000185"
     CDS             complement(61051..61500)
                     /gene="nusB"
                     /locus_tag="IV228_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8716138.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(61493..61918)
                     /locus_tag="IV228_000186"
     CDS             complement(61493..61918)
                     /locus_tag="IV228_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8716139.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(62164..63228)
                     /locus_tag="IV228_000187"
     CDS             complement(62164..63228)
                     /locus_tag="IV228_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8716140.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(63400..63645)
                     /locus_tag="IV228_000188"
     CDS             complement(63400..63645)
                     /locus_tag="IV228_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8716141.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(63784..64158)
                     /locus_tag="IV228_000189"
     CDS             complement(63784..64158)
                     /locus_tag="IV228_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8716142.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(64241..65155)
                     /locus_tag="IV228_000190"
     CDS             complement(64241..65155)
                     /locus_tag="IV228_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8716143.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(65176..65979)
                     /locus_tag="IV228_000191"
     CDS             complement(65176..65979)
                     /locus_tag="IV228_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8716144.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(66012..67001)
                     /gene="manX"
                     /locus_tag="IV228_000192"
     CDS             complement(66012..67001)
                     /gene="manX"
                     /locus_tag="IV228_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8716145.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(67104..67595)
                     /locus_tag="IV228_000193"
     CDS             complement(67104..67595)
                     /locus_tag="IV228_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8716146.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(67786..70620)
                     /locus_tag="IV228_000194"
     CDS             complement(67786..70620)
                     /locus_tag="IV228_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8716147.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            complement(71027..71320)
                     /gene="rpmA"
                     /locus_tag="IV228_000195"
     CDS             complement(71027..71320)
                     /gene="rpmA"
                     /locus_tag="IV228_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8716148.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(71333..71677)
                     /locus_tag="IV228_000196"
     CDS             complement(71333..71677)
                     /locus_tag="IV228_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8716149.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(71704..72012)
                     /gene="rplU"
                     /locus_tag="IV228_000197"
     CDS             complement(71704..72012)
                     /gene="rplU"
                     /locus_tag="IV228_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8716150.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            72294..72935
                     /locus_tag="IV228_000198"
     CDS             72294..72935
                     /locus_tag="IV228_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8716151.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(72997..74628)
                     /locus_tag="IV228_000199"
     CDS             complement(72997..74628)
                     /locus_tag="IV228_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8716152.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(74751..75110)
                     /locus_tag="IV228_000200"
     CDS             complement(74751..75110)
                     /locus_tag="IV228_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8716153.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(75269..75342)
                     /locus_tag="IV228_000201"
     tRNA            complement(75269..75342)
                     /locus_tag="IV228_000201"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75306..75308),aa:Arg,seq:tct)
     gene            complement(75651..76334)
                     /locus_tag="IV228_000202"
     CDS             complement(75651..76334)
                     /locus_tag="IV228_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8716154.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(76462..76980)
                     /locus_tag="IV228_000203"
     CDS             complement(76462..76980)
                     /locus_tag="IV228_000203"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8716155.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT        22089 a        16225 c        12757 g        26030 t
ORIGIN      
        1 gtgtctggtt cacccttttt atctgctgca tctgtccaat ccacaggata gccaactaat
       61 tggtaaccaa ttccaccttc tgcttgtgtg aatggccgca acaaaggaaa tccatcatct
      121 gttttcattt tttcaatggc agtcaaagca gctcgattaa taataaagcg tccctttttc
      181 atcacttctg tcactggtgt atttttaaat tcgattaatg catcatataa tttttgccca
      241 gcacctgaag cagttagatc taaaggtttt ttaaatgcta cagccttttt ggctaatgca
      301 ccaggatttt catttccagc gtcatcacca ttgaacatat aattgatttc tttacgcaca
      361 taagcttttt tcaattcttc cacaacaata tcttcaactg gaacaccaga catttttaat
      421 aattttttag ttactgttgc caaagcatcg aattcggcag gattaagcaa aatttcatca
      481 aactgaatag ctgtttcagc aatatcagtt gaacgctctt tcttgtttac attcgcatct
      541 gctttcttca caagaattgg atatttgaca tctcctgatg ttcgcaccac tgttccgtat
      601 ttacgaagta aattttcttc ttgagcataa gtaataactt cagatgcaat tacttctggg
      661 acagtaactg aaccgttgcc agcttcaatc cctaaagctc gagcttctgc ttcagaaata
      721 tttccagcta caaaattagc aaatgctttt cgtagttgtt gatctttttg tttcttggtc
      781 atttttgcac gtgcctccaa tccgttctta attgatccaa gtaatccatc tcgttgctgt
      841 tgagtaatca ttccagaacg attttctgaa ctatcttcac tgtctgaatc ttcttcttga
      901 tcgttgtctt caccagaacg gctttcgtct gaatccgtac tattcgattg atcatctgta
      961 ttgtcgtctg tttcatcagt tccagaatct gcgcctaatt cgtctttaat tccgttcaat
     1021 tcatcaataa cactgtcaat ttcttctttc acggcttcta aatctgcttc gcgtaattct
     1081 cctgattcaa ctttttcacg taattcagtc aatcgttgct cacgacgtgc cttcattttt
     1141 ttcaataatt ctttatccat gtatttttcc tcctacgctt ctagcgcttg attaattttt
     1201 ttgattaatt tttttctttc ttcgacgttt ttttctaatt catcacgact tcttaatgca
     1261 gcttctgtat cttcgtatgc tggcaaaggc acaatagaaa cctcgtataa ttccacttca
     1321 tggattgttc gaagcattgg ttcagaattg tagtcccacg tttcttcagt cggcacaaaa
     1381 ccaaaactac attggttgat gtctcctcgt tccatagatt taactaagtc ccttgctacc
     1441 gttgtgtccg gtaaatcaac ttcgaatttc aaaccacgtt catcttcttc aaggcgaagg
     1501 gttccgcttt tagtacgtcc tagcacattg gaccaatcgt gattgaacaa acaacgaaca
     1561 tccgaattac taattgcctt tgagaatgca ccaggaacaa tcacttcgct taagtcatcc
     1621 cataaaagtg tttgactgtt gaatacagca gcgtagccgc taatggtcct cgtatgagtt
     1681 tcttcatcgg aacgtgttga aagattggtg atgtcaatcg tgcgaatttc cttcttcttc
     1741 atttccatca cctcctttca agtcttgatc attcgttggt aaggaatcat ctgttgcatt
     1801 tttctggcca atcctagata agtcatttga aatatagata gcttgtgttt ctggagtatt
     1861 ctgtttagga aaaccaagca tttctgccac gttatctgga cttgtaatcc cggtacgaac
     1921 gatgttgtag ccaatatttg tttttgttga gtaaggaaca aaatccaaaa tattaatttt
     1981 ccattccact ctatagccag aattaggcat aaaaaaaaga gcggtgtaat gttcgctctt
     2041 gtttttcaat attggtttga ttgctttgtt gtgcagatac atcatcgctt tttcaatatc
     2101 tgtcttcatc agcgattgat acgtatcaac attgattcct agaaattttc ctaagtcttt
     2161 tttgtaaaca cctaaataat tcaaaattgc cgcgtcatca acaggacttt ttaatgtctc
     2221 gatggaatat ccttttccca aaggaatcat cttaacagaa tgatcactct cattttgcgt
     2281 tccttccagt tgatccaata tagctttgac aatttttgtt tgggcgctat tatttggatt
     2341 gatgtgggcg tctagtttaa gcatgaacgc aagtaaaccg cctttagtat atttgtccgt
     2401 caaaactttt tcagcgctca gaacgccttc tagagtgttt cttgcaagat caattattcc
     2461 agcacctttt aatgaatcag ttccgatgtt cttaatgtgt cgaatcattt gaccaggtat
     2521 tttttgacca ttcatttcaa attcttcttg aagtcgttca ttgattttag ttgttacacc
     2581 gtacgccaaa tgaagctgat ccctatccgt taatgggaat gtctcaccat tgatcaataa
     2641 agtatttgtt tccaatttgg taaattcgaa tcctgtcaaa taatcattgg gactcttcaa
     2701 aatatttagt aagtgatgat ctttgacttc gttgccgtcg ggaccaataa caattggtgt
     2761 ggccaatgcc acctgattag atatgtcttg aactaattca taaacatcag aagattccat
     2821 gatagatgaa tccgtcacat atctttgacc ataacgtgtg tagtgtccaa aaatatcctc
     2881 gatatatcca cgtttttcca taaaagaata gactgcattc gataaccgat cacgtaattt
     2941 caattttttt caccgccttt ctatttatct atagatggaa cttaggtaat catctaactc
     3001 atctgaatca atatctgtca tttgattcat cgtttcctta tgaccacaca aaaacgccac
     3061 gaacccatca atctttttct ttgattgacg tttacttggc gctttttgtc cattgatgtt
     3121 agtaattgct acaacgttca aagtgcaata aaggaataat ggattatcaa attgaattcg
     3181 tttctcataa aacaaccgtt cgacatcgtc aaaaggagca ttcaatactt taggatattg
     3241 agcaacttca acgcattcca atcctaagtt ttctaatttc tcaacaagtt tgtcactcat
     3301 cgctggatca taattcactt gctgaatatc atataaattc atgcagtcct ctatgtactg
     3361 caaaatctga tcttgatcaa tcatttttcc gtcgcaaaat tcaacgaaac cttgttcagc
     3421 caaatcactg taaggaacat tatcttcttt ttctcgaaac tctaaatctt cattcggaat
     3481 aaaataaagt tgctttactt taagaaccgc tttaccatct tcatcccatg ttgggaagtt
     3541 taaagataca caggtcaaat ctcgtgtacg tgataaatct aaaccgatat aacatggttc
     3601 gccacttaaa tcaccaagtt cttgtgtggt aactaaacat ggctctactt gatcctgttc
     3661 aaagaaatta tccgcgccgt tcacaaatac atccaagtgt ttcgttagaa attcagcttt
     3721 ggaatgagct gaccgttgcg ctgttttaaa tgccgattct aaagcagata aatcgactga
     3781 tattccccag ttaggattgc acatttccca gacttttcta tccgtccaat cataattttt
     3841 atttggctca tagattagaa caaagtttga atcattatca tcacgtttca acacttcttt
     3901 tgcttcgcga tatacacgca ttccaacaga cgacgaacct ttcccagcag ttgagatatt
     3961 aaacatcaat ggctgtggta aagatatctg agcagactta aaattgtcat actgttccat
     4021 tttctcctgt ttatgcaatt catcgttcaa tacaaaatat ggattggatc cctcgatatt
     4081 atcaatattc ttggtttgaa caataaattt atttgagtaa gccatatctt catgaatata
     4141 gtcataagta atactagaaa cagttccctt tggcccccta aatattttag ttccttcaag
     4201 taggattgga ttgtttaaga ttgtaactgc aaaaggtttg gcagcatatt gcgcttgagc
     4261 aaaatcagat gcgcaagcat agcaatcaac tgataacgcc ccttctccgt acattgcata
     4321 tcctaatgaa ccgacagcaa tcaatgtttt accattcttt tttggaatct ggacataagc
     4381 ctcacgagta acgcgtacaa tttggccttt ttcattttct ttaacccaac cataaatcca
     4441 agaataaata aatttttccc agggttctaa aagaaacggt tttcccacca tttctccttt
     4501 tgtgtgaaca ataaatgatt ctacccaatc catcatttca ttcgcgcgat ctacatcaaa
     4561 ccaaatatct tttctttttt tccactgata ccagcgatct attgctaaac gaacggtctt
     4621 aggatacttc ttaggtttct ttcttacttc ttttgcaaat aaatcagcat aatttacacc
     4681 aggttcaatc atttttcagc acctgccttc ttacgccatt tatttctgtg cgctgctagt
     4741 tcatctactg gctttacttc tgggcgtgta atttcttcat cttttcttgc ggtcgatcct
     4801 ccagcaatta gtctaccagt ttttgctttg tttgttaatc ctaataaatc tagagctttc
     4861 atcttcttat ctgcccaagt ttctacttgt tgtgccaatg gatgttttga ttgattagta
     4921 gctcctgatt tattcgtgaa tttttgcgtc tccggaaatc ctttttcctt ccacaataaa
     4981 tatttgtgtt ggtaaatttc aaaaatatcc aagtatgatt cgattaatgg atcaagagtg
     5041 atggtgtaca aatcagacaa attcattatt tctaagatac gagctttttc agcacttact
     5101 ttttcatcaa taatcgcttt acgttgcgct ttagtcgtca tttttgtata cacccccctt
     5161 tgttttttga aaaatttgac ctaacgatgc gcgtgactcc ccgctaccct atctccccac
     5221 gcgaaaaaat tttgaaaatg aatagggggg cttcaattga aatacgaagg aaaaactttt
     5281 ttgtcttccg tttcgttttc aacaattgga tgacattttg aacataatag catgagattg
     5341 tttggatcaa gcttaagcag ttcgttgtct ttgattggta caatgtgatg gacgtgtgct
     5401 ctctttccaa atatgaactg accacaccgc tgacaatgac caccttctct ttcataaata
     5461 aattgacgca tatctttcca tgcttgcgtt cgatagaatg gtttgttctc atgatgataa
     5521 acagactttg cttgctgctt cttcttgcgt gatctgcttg atctcttgtg ttcagtacag
     5581 tagataccct ttgctatttt gttcgtgcat ccgtcaaact gacagtattt cattctactt
     5641 cacgaataag atttatgata tcgccttttg cacggatagc accaggaata tcaataccat
     5701 gtttcttagc atatgcacgc aattcttttg cagtcatatt gtccagttca tccgtatctt
     5761 cagtggactg atcattagta gcttcgtctc catcaaaatt agcagcagtg tttccatcgc
     5821 tatcaagaat ttcactgcta tgagctatga gttcgccatt gacagcaaca aatgatttgc
     5881 catcactcaa aattcctttt tcttctttta cttcaaaatt tggttcttga tctttcggaa
     5941 caactactgt ccttttcttt tctgaatccc aatactctgt tccactaatg gatgttctaa
     6001 ttttgatcat tgccatttta attctctcct ttgtaatttg tacggattac ttttgcgccc
     6061 attcgctcat accattcaat cgtttcctgc agattgggta gagtgtgtga caataaagaa
     6121 ataactaacg tattcattcc agttctgtaa tcatcattct ccacttgcac atgtgcatga
     6181 ttaccattcc aattttctct gtacggacta ttctctgctt caattgcttt cttataaact
     6241 ttagccaaac ttgattctac ttcaatacta agtaccgctt catgaaaatc attcatttgt
     6301 ttactctcct atcagatatt ttgtggtatg atttataaaa aagatgcgag gtgttttaat
     6361 gcttcaagaa gttaatatta agtatcgtga tcgtcaaaat acagagacaa tttatttgcc
     6421 aaatagttgt ccgcattgcg gaaatataat gactccgtat gtgtattttg gggtttcatc
     6481 atatagtgcg ttcgataatg atagaatctt tggcgtatta gtacagtgta taaatgatga
     6541 gtgcaacaaa ttttatagtt tacaatttat aaatacgttt aatgaagatg aaggatattc
     6601 tgaaaatatt ttaattaaag attattaccg tccgccgatt aaaatagatt taccagaaaa
     6661 tattgaaaaa gtttctccta catttgttga tatttatact caagccacac aagctaaatc
     6721 tgagcaattg gaacaaattg ctggagttgg ttatagaaaa gctctagaat ttctaattaa
     6781 agattatgca atatctaaaa attcagagga tgaagataaa ataaaaaaaa tgttattagg
     6841 taaaacaatt gaaaattatt tgaatgattt tcctaaactc caaagattag ccaaagctgc
     6901 tacttggatt ggtaatgatg aaacgcacta tgttcgtaga catactaata aagatgttca
     6961 tgatatggat gcatttatta aatcagcagc gcaatttatc gcggctgatt atgatgctga
     7021 tttagctgat caatttataa atggaaataa ctttatgcca actactaatt aaaagaataa
     7081 aagagccaca gataccaatt aataaaacgg ttgggatatc ttggctcttt catatttttt
     7141 tgacactaat agaatatcat gctgctaaca atgtttacat agctacaatg accgaacatt
     7201 tagtgaacag tgtaatttta gaataaagag agttgttctt tgtacttctc gctattgtaa
     7261 aaatcaattt cgttttcttt tttcaatcgg tcagcttgtc tgtgttcata ctcatctaaa
     7321 aagttcaatg tctttctaat ttctgcatgt cgctgtctga catacgatga gctataccca
     7381 acagattcag caatatcttc taagctcatt tgatcaacat atttcatctt aacaatttga
     7441 ttatcgatac cagaaaagct atctatcagc aataacattt cttctttttg ctcgatcaac
     7501 aattccaatt cattttctat cttttggata ttctcttcta aagaagaagt tctcgaattc
     7561 ttttcgatgc ggacgtttgc taaatcacca cagacccatc gattcaattc aagcttactt
     7621 ttattaagat tccactttaa gtaaagaatc tgctcatcta gttcttggta atcttttaac
     7681 caatgaaatc tcacaaacgc caccccttat ggtagaatag tattgcgctg ctatctcgaa
     7741 aggggtagct tttttatttt ttcttagccg ttccattctg gattgccttt tgttccaaac
     7801 gtcgtttttt cttcttaatc tttgattttg tttttcccat acaattctcc tttccgaaaa
     7861 cacattttgt tcgattttta atactaaata aaagttggcc agaatttttc gttcgtcttt
     7921 actcatcttt ttttgaataa gattactagc ataaaaatag ttagtaccac gaaaatgaac
     7981 aggtaaatca attacgttca ccttcttcat gccagttatc agccactaga atctttgcgt
     8041 tagtgccgcc accgttttct tttttgtatt tttcaaatgc tttataagca gctgtttttg
     8101 atttatagat ttttccggaa ccgactggga acatccatgt gccctctgtg atagctaaga
     8161 tgtatacttt catttaattc acccctttaa tccccaacac tacatatccg gacttctgca
     8221 tgtaatcagt aatataagtg atctctgctt tgtatgagtc accagtgtaa ctaaatgttg
     8281 gtctcgttag tccaggtaat tcatgtttaa tgtatttttt atattcacgt agtattaact
     8341 gatcaccaac tttgaagtca cgatcattct tgcggatctc gaattgtttg cgtccgctcg
     8401 tcactgcttc aaaatattct ggtagtattt ttagttcgtg gatcattctg tttcctcctt
     8461 caaaattcgc tcatcatcaa aaagtgaatc tccacaaaat ccttctccca aatatttatc
     8521 gttcatgtat aagtcaacca aatatttgtg atctgcttct cgatattcat taatattaac
     8581 gatctttatt ttgtaaacat taccttcgtc gctcactatc ttgaattgtt ctctgacttt
     8641 caccaccgtt cctcctcttt ttgaaaaaat tttagtgttt ttgtaagacc ccctaaatca
     8701 gcttttttat ttgatataat acttgtatcg attaatcaaa tcgaaattaa atttaaaaag
     8761 gtgatttatc atgaatatca acaatttagt aaaactcgtt gaacaacgtt ttaaagaatc
     8821 aggattagaa caattaaaaa aagataattc tccagaatta ttttctaatt ttaccgaaga
     8881 cgacattcct actcttaaac aattaaaata ttattctgat tattggacgc aaactcgtga
     8941 gaatgaatta aatttagaaa aactcattat taaaaagaaa tagcaactcc agcccacacg
     9001 gctggttttt tagtttcaac ttccctcctt acgggtcaaa atcatcggtt ccggcataat
     9061 ctgatcatcc tttagcaaca aatcaggata ttttttcctt agcttcttat tgttcgtgta
     9121 agttttcaat agccattggt tggcttcgct tttgctttct ccagaaaaat aaattttccc
     9181 tgtttggatg cctgcaagtt gaatcatcat tttcacctca aatcaaattt tttctgctag
     9241 atggagcaaa agcaatccga catcaagaat cttttgaccc attatttcag ctactttgct
     9301 cggcttaact ccttgagaaa agagcaagcg tgcttgctct atctcttcgt gagtccaaat
     9361 aaatttggct tcctcaagga taattactgg atcagttgct ctcattgttc ctctggaaaa
     9421 atgatgtcat tcttatgctt ggaccaggta tctgcatatg gaacaaagta ttgtctagct
     9481 aattcgattt gatcaattaa agcagtttct gataattcat ggtctgcagc aatttcattc
     9541 atactatcgc cttcatctaa acagataaga ccgttgacgt tatcgatttt tacatttgtg
     9601 actgcttgac tttctccacg ctcataatat tctacagttg atactgatac atcagcttcg
     9661 gagtttaatt gcagaatctg ttactgccgg gataatttaa tattcttttc ataattcacc
     9721 ttttttccta ctgacatcga atatttttca tgttatgatt agccgtgaaa ggagagctat
     9781 attatgtgtt tacacatgat gtccttagct cattcggttc tttggaagct tttttggaaa
     9841 taactctcag gttcttgacg caaatctcat ttattctttg aaacaaaaaa cttccatttt
     9901 ccgagagctt tggatcactt caaaagagat ttgcatttat atttctgtga ccttgagtcg
     9961 cgtaaagaac cttacagcaa tggcggcaat tccactttca ggaaaccgcc ttttttcatt
    10021 gatattggtg gttagcggac tacccatttt ctttcggcag tgccaaacca caaatgatct
    10081 gtgttgatgt ttaggtagtt gtataccaga taagctaaat ccaaagtgcc tgcatcttca
    10141 agacgacaat attccaaaaa ttctcccata gctggacata aaaatcctgg agctatcata
    10201 tcccaacagt taatagtgca aagaaattcg tttagcttct gaacgttttc tttggtttca
    10261 cgaattacaa catactccaa ttcttctccg ctggcagagc attccaaaac ttcaaattga
    10321 ttaagaaatg gtagaacata tgcttcaaac atcaacattc catctttctg tttttgcact
    10381 tgttttgcat caatcatgtc tatcctccta ttcagttatt tcttccatta atggaattat
    10441 ttcaggtcac tggacttaac gaaaacacca ttgaccatct ttccagtccg acctttgatt
    10501 tcgtcataag caaaatccaa gcactcgtat aaatccatat cattttgcat tgccaagatg
    10561 atcaaggtta ctactacatc gccaattccg tctcttaaac catgttcatc ttttctagct
    10621 agagaagcgg caacttcccc gatttcttcg accgttttta acatttgctt gctgaaatcc
    10681 gcttgatcca atcccttatc ttttgcccac tgctctactt tcgcgattag ttcgtccatt
    10741 atttctcctc cacataccta aactgtcttc cttttgaatc aatccataag ctcctagctc
    10801 tatcccaaat aatatttttg ctcagaccag taatttcaga taactgttta gcggtacccg
    10861 ttactagaat tcgatcacca tgccagattg caatctttct cggcgttctc cgtttgggct
    10921 tttcagtcca cattgattta ccgagctttt ggacttctgc aactatttct ttgtcttctt
    10981 gccaagattc tgacttggtt aattcagcaa ttcttttcat tgctgctttc ttatccacgc
    11041 tcattcctcc aatcgatgga tttcccttct taaattctct atgtgcaaat cgattgcctt
    11101 tctcgccgtt tcattgacca tcactgcctt tgttcgttcc agatcgtcaa tctcacgctg
    11161 aaggcttcga atacgcattt gaatcacttc ttctgttgtc atgatgattc ctccacgtat
    11221 ctaaacgttc tcttcttagc gtctgtgtag ccacaccttg ctcttttcct cacgattttc
    11281 tcgtgcaagc ctgtgagact tgctaactgt tcagctgtcc ctatcactag aagtttgtca
    11341 ccgtgccaaa ttgcgatttt tcgtggtctt ggcttgttgc tcttgtctgc ccacatcgct
    11401 cttccaagcc tcatcacttc tgaagcagct tctttgtcat tttgccaatc ttctgaataa
    11461 gtcaattcga taattcgctg cattgccgct ttcttatcca tcccgacatt ctcctttcag
    11521 taatttgagt acttgatcaa gtgcgctctc acgtccgccg tggaacgtgt tgagccactt
    11581 gtcttcgtac gatacacttt gtcttaaagc ttcttgatgc attagttcaa tctgtgctgt
    11641 aaatgtcttt aggtccatct gattacacct gctcaagttc attaagatgt ttttgcaagc
    11701 ctttaacaca atcaacaaat agtaatttgg tatatgctaa atttcttaat tgtgttgtat
    11761 cgatataaag tgcgaaatag tatctgagct tactccaact cgaacgatca ttcttaattc
    11821 cttcgattcc agcttcttcg agttgatcat atacgtctct caggatttct atttcctcac
    11881 cagttttata ctttgctatt tcattaatta gttctagata atcgattttc aatttcccac
    11941 ctcttagaat ggtgcttttg attgtctatt agctcgttct agcgcttttt tcttgagata
    12001 ggcttcttgg tcgattgccc actcaggaag cttctctcgt cttcctgtgc gcttgtatcc
    12061 actgcttgcg ttcttagatt cacttttttc ttttcttgcc cagcttcgaa tagttgccaa
    12121 atagttttta taagtcttac cagatgattc acaatactca gatagccgtt ctattcgttc
    12181 ttgatagtca ttagggaatt ctattttgag tttctccatc tgctcatctg acaaaagaac
    12241 atttttatac tctccgtatt tatgacggac gggcttagcc ttcgattttt tcgaaggcgt
    12301 tacattctct atatctttct ctaactctat atctttctct aactctatct ctaactctgg
    12361 tgtagttttg tccggacatt tgtccgacat ttgtccactt gttattaaat tctgttttgc
    12421 ttcctcaatt tcttttctgt attttctctt cctatcagct tcagttgaag atttcccaat
    12481 aaaactttgt atatcagaca tataaattgc tccgttatct aatacctcaa taagttgtaa
    12541 atcacgaaat atttgtactg ctttttctac aactcccaca gagtgtcttg taatagtcgc
    12601 aagcattgta gaattgaatg gaatcctgtc attaaacatt agtttgcctt catgtttcag
    12661 acttcttaaa taaagtttga gaagaatatt agaataaatg tatccatctg gcatactttc
    12721 taaaagaacc atctcgtcgc tatcgaaaaa gttctctttt agttttaaat agtagtatcg
    12781 tttgttgtca gccaatattt tttatcctcc tattctaagt ttcttaattg tttcctggtt
    12841 taacttgatc cctttgattt gatatttatt tttgaaattg atcacaccta tcttgtgttt
    12901 ctccgtgtga tggattctgc agagtgctgc aaatgtgtac tctgaatgat caacttcttt
    12961 gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgata tcagctcctg ttttgccaca
    13021 gatgcaacag actctttttg tgatgcattt gtagaagtaa tattcttgat tcgctggtaa
    13081 aatctcatag ccttctttga aaggaatatg atgttcaaag atgaaatcta agatgatatt
    13141 tgctaagacg ttagcatcac tcacagtcgt attcgattca tctttgaggc ttattttgcg
    13201 ccctgtgaca ccttcaaagc ggaagtaaaa gaattccttc cagaagtccg ttggcatgcc
    13261 tgtatcgatg aaaatatcgc ctatgagtgc atagatgaag tttcgttgct gtactgtgaa
    13321 acgtctagga tcaataaaac gaatttcaat aactcgatca ccatcgtagc cgtcatacat
    13381 cgtctttagt cgatcaatgt tcacttcctc attgatggtt gcacctatgt cttttccttt
    13441 gaactttttc agaaccgctg aatatgaatc gattaatggt ttaaacactc atatcacttc
    13501 ttatctaatt cttttctctt agctgctatt gctcgctcca tcaaggcaca ttgctcatag
    13561 cttaactgtt caatagtttc aacgttatca gctaagagcc ctaatttatc tgtctgctca
    13621 ttaacatatt cgattaaggt tttggtcata tctttaccca tctgctcatt gaaagcttct
    13681 agaatcgtct ctagcatgtt taatttcttc gtatcgattc taggtggtgt tggaatatct
    13741 tccccttgaa atacatataa tcccagtccg tgtagagcca atgctttcac aaagcatcgc
    13801 ttcaatgagt tattgatttg cattgcattt ggtttaacaa ctggttggtt tcgataatct
    13861 aaaacaggaa ataactcggt ttccgtgtgt cctttaaccg ttactgagac agatacataa
    13921 gtccctgttt catccataag aaaaggttta tattcctcaa caagaaagtc ttgatgagtt
    13981 ccagaaacaa ccctgtagtg tttatactca ttaatagtta ccgttgcctg tggatcattc
    14041 tttttcataa tctcccacgc gtgagcccaa gataaataat caaaatttcc ttttttcttg
    14101 agtattttat ttaacttgcg actaaaaagt ttttcaaaag tcgttgtccc tttgttttca
    14161 ctcatcaaat tctgcctcca tttcagcaat gtatttctta cctgatccgt aataagagat
    14221 atcaatcaag ttatctctgt catactcttc tagcgcatca atcaagccat cttcgatgac
    14281 atagatatat tcaggttttt tggacttcct cgataaatgg ataagataga catgatccca
    14341 aatactcaca aaatttccca aatcgtcttg atcacatgct agttcttcat ccgtcaaaag
    14401 atttcgtctg atttttcgat tatttgtttc cttgatattc gatttgcccc aactaggatc
    14461 agtcaaatat tgatctagag tggaaagttc tttttccata tgctaaaatc tccttatgat
    14521 gtgttttctt tgtgactctt tgcttgccgg cggagtcact tttttatttg ttgccatgct
    14581 ttttgcttgt caatatgttg ttggcttagg atgattggtt tattgtgtct ccaccagcga
    14641 ttagcaatta ccgtcccgat tcttagcgct tcagctctat tcattttcat caccgaaaag
    14701 tctttgttgt ctgttcagtt gatcgatttc catgcggatc gcagtttctg gtaaccacat
    14761 ttcaataaat gaaacagtat catcgaatct cttacgaggt aactcgccat atcttgggat
    14821 tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat acttttttgc tgattttttc
    14881 ataagctcgg ctttctttac cccctagaac ttccataact tttatatttc ctttttgttt
    14941 aatttcaaac tcttgttgcc cactaattcg catagtatct ttaagcatgg aaacatcttt
    15001 tttaacatct ttcatttctt ctagttggta gatcatcata tcttcaattg tttgaggaac
    15061 agtattcttg cgaataacat cttccatttc gttgaatgct tcaatgtatt tttgtttgaa
    15121 gtaaatagct ttctttcctg taaaacccat agccaacaag aaaaaaccat ctctactaat
    15181 gaagaaaact cgtcgatttc tgccgtatga atctggttca ttaccttcag aaaacatctg
    15241 tccaaaattg gacacatctt ttttcagtgc atcaatatct cttaaaacat gttgatgctt
    15301 tttctcaaaa ctttctgcaa cttgcaaact cgtagtcaca gcttctttat ttttcaaaat
    15361 tactaattct tgcattattt cttctctcct ctttgatata atgtagataa aaaatggtgg
    15421 tggtataaat tgagtcttat tgactttatt aaacaatttg actggaataa cattgttaaa
    15481 accattaaag attttatttt gcctattacg tctctagtat tttcaacatt tgctttttat
    15541 atctcctata cagatagaaa aaacaaaaaa tttaatttaa aattggattt cttcgctgaa
    15601 tgcgaagaat ggttgataga tagagaaagt gattccaaac cagatgttta ccatcaaaat
    15661 aaattcagga ttatagattc tgtattgtta acaaataaca gttctttacc tgttacaatt
    15721 attgagtttt cgatttctgg aattcctgat catttaaatg cttttacaat gattggtgat
    15781 aattattctg taacggtaaa atcaatgtat gacgaacttc cacatggtat acgtgcgtat
    15841 tctggtaaat ctttaaaaaa aggtgctgat ctatcaaaat ttcctccgtt gtcattacct
    15901 attaccattc caccgtatga gtcaaaaatt actacgttgg tttttagata cgatgaatct
    15961 ttagtcgaaa aaaacattac aataaatgtt cttacaagta gaggtactgc taaattcaat
    16021 agatttgttt cttcatctca aatttcacag cttgatactg gctatgcccc tccgcaacta
    16081 gatgaatttg attaggaaat tctccagttt cttttacaaa ttctttgatg agatcattaa
    16141 aattttcttt caattgtttt tcgtttatag tcattaaact ttttttcatt agtcagtccc
    16201 tcccgactgg cttttttgtt ttgtactcag cttcatccag ccccataaaa atccaaacca
    16261 tgtaaacaat cgttcctatc aacgcttgtc tgcttcccca aagtcctaaa gcgtagatga
    16321 ttagcggtgc gctgaatact agcgctctat taaattttcc catccgcttg cctccttaag
    16381 atttccgaaa aatttgtttc taaaaattca agtgttttac ttcttaaaaa tagatatgtg
    16441 tctcttcctt caactggata atagacaaat ccatttttat ttttttcgat atcgataata
    16501 tttctatatc ttgggttctt taaaactcta gaagtaaacc aatcatattt tctgttaatc
    16561 cgttctagca cttctggcaa cgtcatccat ctaccagtat catcagcttt ttttaactcc
    16621 tcataatcca cttgggagat aattacatag ccttctggaa ctgggatttt cgcttctaga
    16681 tattgcatta gctgttcctc cctttacaat tcgtacatag taataatcga atctatgatt
    16741 ctatttgctt ctgcagaagt ctttttaccg tttaaaatta aagataagta gcttttgcta
    16801 attccaaatc tttcagcaag catggtgtaa gttaagaact tcgaactttc gacatattct
    16861 ttgatttttt ctctatctcg ttgagtgatt tctgcaatgt cagtcatact aaaactcctt
    16921 tctaaccaat ttcctctaaa tccatttgag ggtaatagcc ctctgcgatt aataagttgt
    16981 aaataaacac tcgccctttc tgtgtccatt tggtattcat tacaacttta gtgccgccat
    17041 cggctttcgg aatctcactt gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt
    17101 aaagtatcca ttgtccgcta actttatgtt gaacacctaa ctcgtttagc aatttgttta
    17161 gagcaattgc cgacattccg taatcagctg caatttgaga agtagctacc gtatctgtcg
    17221 atgaaagaat cgtatctaag tagctaatct ttggttcgta ctcggcaatt tgttgttcga
    17281 gtaattggtt cttttcttct aaatctgctg ctaaacgcaa tgcttgtgca aaactttgag
    17341 gtacgtttga atagctacct gtttttctga ttgttggaag gacttcttcc attacccagg
    17401 cctcaaattt ttcagcgctt ggtaagttcg atttaataat caagcggtaa acatctgatt
    17461 ctggaataac tttgaatttt tgacgacgac ctaacgaatc gttaccccat gtttcaattg
    17521 attttttaca atgatcattg gtggctttac tgggattttt gtatcccaaa gttttggcaa
    17581 catcgtttgc tacgaaatac ggaatgtcat tttctagaaa cgttcttact tcatgttgtt
    17641 caaaactaaa aatttgtggt gtgttcattt ttgttcattc ctttctttga tataattttc
    17701 ttatcagtaa gtggtctact gaaataatgc ataaggtggt gaataagtat gaataaaaaa
    17761 actgatgaaa tcgctgcgga tttagttgtt gcatggctaa atcacgaatc acaagtatct
    17821 actaaaggcg aatcaatttc gccaaaagaa attgctcaag cttatttaga tattcattat
    17881 gctgttatct atggtcagct tccagaagac cgtaaaaacg atgactaaac gtgagctaaa
    17941 atctctgcta tggctgcaac catggcagag tcttctttat ctaccgcgtg ttccatcgcc
    18001 acttttgctt gtttcagtat gtctaatttc aaatcttcga tttttgccga gactgttttt
    18061 tccatattct tctctccttt cttttatatt cgtaaacaaa tttaacaact tttaaaaaaa
    18121 ctgcgttgac taaattaaca tacagtgtta aaatcagaac atagttaaat aagacatata
    18181 aacattgatt ttaaaagctt tcttggcggt tggcatttat taatcaatag tgttttttgt
    18241 tgtcttttta gttgttaaac ttgtttacaa gaatgatatt aacacctttt gttaaaatag
    18301 tcaacgaatt ttaacaaata aagttaaata ttttttcttg aacgaaagga atattgatat
    18361 gataacgttt gagagaataa aagagttagc gaaaaaacag ggaaaatcat taaataaagt
    18421 tgaagaagac ctaggctatg gtaaaaacgt cttatataga cttaagaact caaacccctc
    18481 tacagaacgt ttgcaagaaa ttgctgacta tttcgatgtc tctgtagact atcttctggg
    18541 gcgcgaagaa aaagaaaccc ctaaacatgt ggatttatca gaaaacgata ctgttttttc
    18601 ttttgatgga aaagaaatat ctaaggagac aatgcgtaaa gcgattgcaa ttgctaaagc
    18661 tttagaggaa aatgaatagt tggagtgatg ggttgtatgt atttgaaatt gaaagaaatg
    18721 ttgagcgaat acaatttgaa agtagtttac atggaaatga aagagcctgg cttctattat
    18781 ccgaagccta gaatcatttt tttgaatgaa aatctatatg gcgaaactgc agaagccttt
    18841 catttatctc atgaacttgc gcatttcacc gcttctcatt ttgaatattc agttttgtac
    18901 gatacttcta caacttttca ctcaaaattt gaaacagaag cagataaaat agctattcta
    18961 attttattaa atatctatat tgagaacgaa ctgactgatg aatctcagtt taatctcgaa
    19021 aaatttatgg agtattattc tattcagaat aagctcagat acacttgtta tgctgtctgc
    19081 caatgctatt ttaagaaaaa atattcttac gcaaggcaat atgtatgaat acatcaagaa
    19141 ttattaaaaa aaccgatttc agcaaaacat taaaaaagcc cgtgctggca cacggactca
    19201 tacctcattt ctgagatcgc aaatatatta taacaagaag tgaggaatat ttaaatggca
    19261 aaaaaagtta tgggtcaaga cggaaaaatg tataaagtta gtaaaccttt ttacaaaaaa
    19321 gtatggtttt gggtattagc agtaatttta attataatta ttggttctgc tttaaatggt
    19381 ggatcagata gtaataaagc aagtgataat ggtggcgaaa aagtaactaa atcttcaacc
    19441 tctgcttcat cttctaaaga agaaaaaagc gataccttct ataagattgg tgacactgta
    19501 aaagttggtg atgctgaata tacactaaat agtgtagaac taactgatga aagaaatcaa
    19561 tttgaagaaa accaacctgc acaagtagta aaaattactt ataccgtaaa aaatgatggc
    19621 gattcagata tccctgtagg tacagatgtg gaagtatatg gaccagatga caaaaaatca
    19681 gagacatatg ccaacgaaaa tacaatggga tctgttgctc caggaaaaca aatggatgta
    19741 actgctcatt tcacgttaaa ccaaaaggga gaaatagaaa tccacttctc tcctttagta
    19801 tcatttgaaa aagcagctat ttttaaagca actgtataat aaataaaaaa cacgcccacc
    19861 gtccaaagca agcgtgttct aagaaaaaca aacctataca gtaggcttct ttatagtgcc
    19921 tattgtatca gagaaagaga gtagattcaa ttatggcaag attagtcaaa cgtggaaata
    19981 gttggcaata cgaaatttca tacaaaaaag atgacggaaa atacacgaag ataagaaaat
    20041 caggatttaa gacaaaagga gaagcaaaag atgccgccaa cgaattagaa tataacttga
    20101 acaaaggcct taaaggggat cgcaaaaatc tattattatc agattacttt gaggattgga
    20161 tgcaacttta taaagaagga acagtatctc ctatcactta tagaaaatac gaagatacgt
    20221 taatgaacat aaagaaatat atgccagcgg tattgatttc tgatttagat agagtgggat
    20281 atcaacgctt tttaaataag tatgctaaag accatgtaaa atctaccgtt attaagttta
    20341 ataaccatat tagagcgtcg ttgaaagatg ccgtagaaga aggattaatt ccgtttgatc
    20401 caactagaaa agcagtaatc aaaggaaaag attcattgaa gccaaaagaa gataaatatt
    20461 tagattatga tcaatttaaa tctttaatga aactcgtaga agaaaacctt tctgcgcagt
    20521 actcttctcc tatgctcgtg ttagttgctg gtgccactgg aatgcgattt gctgaacttc
    20581 taggattaac atgggaagat atcgatttcg aagatcaaat catcactatt aataaaacat
    20641 ggaattataa attaaatgaa tggggaaaaa caaaaaacga aacttcaaat aggaaaattt
    20701 ccattgataa acatacgatt gatctcttaa aaaagtttaa aatcaatcaa aaagaattat
    20761 tcgagaattt tgaaatcaaa aaccctcata attttgtttt tttcaactta aaaaatggat
    20821 tagtttcatc aaacgccgtc agcaaatatt tgcgcaaaaa attaaaagaa ttagggattg
    20881 aaaagcaatt tactttgcat ggactaaggc atacacatgc atctatttta ctttatcaag
    20941 gagtaaatat acttagcgta tcgaaacgtt taggacatag cagtttagaa actacaatgt
    21001 ctacttatct tcatattgtt cgagagcttg aagatcagga taaagaaaaa atcaatactg
    21061 tgttcgatag tttatataaa aatgataact agtttggcac atttttggca caaatacaaa
    21121 acaaacccct gtaccgcaaa ggatacaggg gttatttata cgttcctgac tggaattgaa
    21181 ccagcgacct atcgcttagg aggcgattgc tctatcctac tgagctacag gaacaatttc
    21241 aataaatcaa ttgtagcttg tttgtttttt acttgcaagc ttatctgcta aaaaaatcag
    21301 cttataagtt aggcaaaacg tttccactag tccttaacag atgataaact agaggtagaa
    21361 tgaaataatg tagggaggtc ctttccaatg aatgataagc caaaagaaaa agtatacgca
    21421 gaacgcttag attcatctaa aaaaaatcgg gaaaagatta cgaaagaagc aatccaagac
    21481 cacaaaaaag aaaaccctaa aacaaaagaa aacaaaaaat tcaaatttac gaaaaaagag
    21541 gatttacttt aacattttcc ttcaaatgcc aaatgaaaaa caaaagggtg gtgacaaaaa
    21601 tattgtcatc acccgttttt tatcaaaaat agtcattaag aattggctgt tgctgtggca
    21661 agattgagct taacgtttct tcatattctt ttgtcacctg tattctttct tggaatacca
    21721 actcttccac tttacgatac cctaataata gctgcgtgat atgttggata cttcctcgaa
    21781 cgattgggag ttttgtttgt tctacttttg agagatgatt ttttccattt tcgattgtta
    21841 cttccacgat tccttcattc catttcgcat aatgatcttc ttctatttgt agggcaaatg
    21901 cagcttgtct ctttttaaat ggataatttt ccaaaaacaa agaaatatct acgatcctcg
    21961 ccatcatata aggtgtgatc attacagaag caaatggatt ttctaataga taattttgat
    22021 cattaccgcc gtagccgcat gaatattcaa atgtttcaaa agcaccattg tgagaactta
    22081 cgaagcgaac aaggctgcaa aaagcatcat gatccaaaaa tccccactcc gcaataataa
    22141 aggaagacgt tgcaagctga taaacgatat atcccgttgc ttcctcatta tcatcaaaat
    22201 gcaaagcaaa ctggtaattg cctttttgct tgaatttata cgtataccac cagtcttcgc
    22261 gcttcagacc agctcgttgg ttttccggca tctgttcata aattcttctg attgcagctt
    22321 ttgcatcttc aaaatccata cgctttattt ttccagattt tttttgataa acggcacatc
    22381 acgactgttt agttgataac tgattttatc aaatgattgt tcatagccat agcgccgata
    22441 gaagggatag gagaatggcg ccagataaga taagcttact ttacgatcac ggcaatcttc
    22501 aaggatcttt tccatgatct gattgatccc gccttgtccc cttgcttctg gataagaagc
    22561 aacatagcca atgccggcca ttaggtactc ttgtccgtaa aaattcactg gaaaaggggt
    22621 cgccatgact tgacttgtta gccgatcctc ctcatcaaaa aatccgtaat tccaagaatt
    22681 ctccgcaagt gtacgaaaac gttctcgcct ttcaggtgta tctgccccat taaatgcata
    22741 agtggctaaa tcaaacattt cctcaatcga agatacacct agctctctta ctttgtttgt
    22801 cattttgctg ccccttccta caaaaaagct agtgaggatt cgcaggaaag atctatcttc
    22861 ctatgaaaaa ttccttctag cttacttcat ttttcaataa ctaaatagta ccatgtttca
    22921 ttgatttaat acgttgcttc gatcatatct agcatttttt tgaaacgtct ccgtttaaaa
    22981 tacatcaaga ttgttcctaa aagcatatca ttcaactttt gaagcatgcc ataagactcc
    23041 attttttctg tgtaatgaac ctcacaggtc gaatcgtcca atggtttgat ttgataatga
    23101 acgacgaatt cattcttcgt tgtcgatgta cgaaacttat aagactggtt ttcaatgact
    23161 tcttcgattt ttatgcgagc ccggttattt tgcgagaatt gtttgacgta ctccaaattt
    23221 ttcagctgtt ttctagttac tgatttgccg gtcgctttac gtacatcgaa aaggacagag
    23281 tccataactt tatcgtaaaa aaacgatgct ggaatattta attttttcgt gatttccatt
    23341 agttattttt ctcctttgct tcttctcttt ttttcccctt catgaagaaa tagattccta
    23401 atcctgttag aaaaagacct gtgaccatac tatacaaatc atacctttgc gaatgtggat
    23461 tcaaactata caacacgatc aaagaaccaa tactacataa aaataaaccg ttttttatca
    23521 tctcaaccat cctatctttt taaaccattt tctaaacagc tgttgattga aaataaagga
    23581 ctgttcccca aggctgtgct ttgattagtt cctttgcttt tttagccgca acttccggat
    23641 cttctgcact tgtttcaaag aacagcttga ttccttgttt ttctttaaat gtaaaagtca
    23701 ttccttcttc attgtatcct tctaacaatt ctttgatttc atcttgttgc attgcactgg
    23761 caattgtaat catttcagtt cctcctcgaa aattcattat gaggaacgaa acttaaagcg
    23821 tcccgctcct cacgttcatc ttattgttgt tcgcctgcgg cagggtccat tttgtttgca
    23881 gagatgacaa atggtgccca aatcaagaaa gtcacgacca tacatactaa tgttacgatc
    23941 ggtgcccgcc aatccgctcc tgttgctaag aatgacaata agaacggcgg tactacccat
    24001 gtgacttgct gcgataccgg agcgactagg ccaagataag tagctgtcca tccaattgct
    24061 gttgctacta ctggagcaat caagaatggg ataaacatga ttgcattcaa tacgattggc
    24121 agaccaaaca tcactggttc gttgatattg aagataccag gcccaatgga taatttagct
    24181 actgttagat aatctgcacg tttagagaaa atcaagatag ctatcagtaa gacgatcgtt
    24241 ccgcctgatc cgccaaacca tgcaaaagca tcgaacgatc cgcggaccca catataagct
    24301 tttccatctt cgattgcatc taacacggct ggtgtaccag tttttccgtt ataacctttt
    24361 tggaaaatat caatattgat tagttgtgct tgtccccaga ttccctctag cactggtgcc
    24421 aatacatttg atccatgaat accaaagaac cagaatactt gaacaaatac tgtgacgatc
    24481 aatactgctc ccaacccttg agataatcct aggaatggtt ctgcaatata tttttgcacc
    24541 aaatcaatga tcagttgccc atcagctaca cgacctacga tataattgat aatagcaatg
    24601 acgtataaag cgatcgtcgc tggtaaaatg gctgcaaatg ctttagatac tgctggtggt
    24661 actgaatcag gcattttgat cgtaatgttg gcaagcatca atttacaaaa aataatgact
    24721 gaaatagctc ccatgatcat aactgtaaaa taagcgtttc cattcaaatg atctagtttt
    24781 agccaacccc ataatccaac tgacagtgtg ccatctgcaa tcgttgcacc aatctcagaa
    24841 ctattgattg catctgcgac atttgaagga acattcacat ctaatgctgc tgataatgct
    24901 ggaccaaagg aaataccttg aatcaaggta gctaaaccaa cgatcccccc agctagatca
    24961 tttactccat aagctcgggc aagattatac ccccaagaaa aggcaaagat caatccggcg
    25021 attgctagtg taccgttcca gacaaaacca ttgattccga tgatgacatt caatacggca
    25081 aagattcctt tgtctgctgc taaactgtca gcataggtac tgttaaactg ttgcggtaaa
    25141 tcacgaataa ttgcattgat cattacagcg actgatcccg ccattgttgc gggcagtatg
    25201 ccgataaaag catcacgcaa agcaactaaa tgtttttgcg ctccgatttt tgcggcaacc
    25261 ggcaggatgt atttctccat ccaacttgat agtccgttca ttttttactt cctcctataa
    25321 gttttttata taaagagagt tctccttata acaaaataaa aaatccgttc aaaaattttt
    25381 cttcttccgt tcaaaaaaga agttgtattt attcgctgta tacgcgacga taaagttcga
    25441 tgatttcttt tgctaaatca gtaaacgcaa tagatgtcat taagtgatct tgactgtgaa
    25501 cagtcaataa tgtcacttgc atatgatttc cttgtgcttc ttgtgtcaac atgtctgttt
    25561 gtgcatgatg cgcttgtata agtgattctt ctgcatcagc gattttccta tccgctaact
    25621 caaaatcacc gattttagca gccgcaatcg cttccattgc atcgctcttt gcattaccgc
    25681 caaacatgat taaccccatg atggcttcta gattttgttg atcttccacg ttgttatacc
    25741 cccaactgca tacttgattt atttcattag attttctgct tgtgcaagaa ctttttctcc
    25801 gttcatcatg ccatagtctt gcatattgat gacatctaat ggaatacctt taggcgcaag
    25861 tttttgttca aattgagctt tcatgaaacg tacttgagga cctaataata atacatcgac
    25921 attcttagat tctaaattat tatctgcatc tgaggcagag accgcaaaaa tatcggcttc
    25981 catccctttt tcttctgctg ctttttgcat ttttgtgacc aataaactcg tactcatccc
    26041 tgctgaacaa actaacatga ttgttttttt tgccattttt aacactcctt ttattttttt
    26101 acacttttat ataacgcaag aagcatgcca ataaaaaaaa gagctgaaat agcgttttca
    26161 tgctttttag ctctttttta tagtgcgcac acagatgttt agtgtgtaaa cagtatgatt
    26221 agtgtgttca tttttttatt ttattttcaa gaaacatttg tgtaagaaat gcgatatcat
    26281 cttctggaat ttttacctga tattttcgtt ctagtgccat caagtttgcc cgaacgatat
    26341 ccatttccaa acgataaatt tttgagaaaa tagacagttt ctcaaattgc cgatggcttt
    26401 cttgtttgat caatccgtct aacatgaagg ctaaatggat aacgattcca gtatctactc
    26461 caggttccac gataatcgac aaatcggtct ggatctgctg aactgtcttt tgcaatgaac
    26521 ggatcagttc atcaatagaa aagacatcgc ttaatgatcc agcaagtgaa tcgacaattt
    26581 tagctgtcgg gatctcatca tcagctatac gctttaaaat atttagtttt tcatcatcaa
    26641 aaacgtcata ggctgaaaaa aatgggatat tttgataatc aacatcaact gttcccgcaa
    26701 ttgctttgat ctcatattct tccaataagg catcgatgtg ttttttgaat gattcacgtt
    26761 ctaaaaactg catctggatc agttctacct tattttgatt aatcactggc ataatacgct
    26821 gatataattt ggcagctact ccttcaccag taaagcatgt cacgattacg gcttttttac
    26881 gctgtaaagt ttcagacttt tccgctttga actgatcacg tacgatcgat tcaaaggaaa
    26941 gctggatatt ttgataaata tcttctaagc ttcttccaac acttgccatc cgtatcgctt
    27001 ctatcacaat catggtactt gtcatcgtga tagctttcgt ccgaatgcca gtctcttcat
    27061 aaatcaaatc actgaaggtg tttaatgatc ccatatctgt taacaagagt aggccattcg
    27121 tcaaatgctc ttgatagttt ttgacatatt gtaacacttc ttcatacatt gtttgaactt
    27181 ccataaccag tggcatatta aaagctttcc ctacttttgt tcctaatagt tcttgtgcca
    27241 tctccaacat gcttgaagca gtctgtctgc cgtgcatcaa tatgagaaca tctactttgt
    27301 tttctttaac aggctcactt tctcctagat tgatagaaag aaacatacta ataaagccga
    27361 tttcatcaaa aggaatctca acttgttctt cttcttcaat gatcgttgaa agatcgattg
    27421 ctacctggaa ttccctctta aaatttttcc gtacgttatt caaatctggg tggacgatca
    27481 tatgaccttc tctcacacga tccaatgtac tttgcagatg caaagcaaaa gcaaaacgag
    27541 ctttttcatt ataaatacgg tctaaccgtt tttcagccac atcatacaat tgattggtta
    27601 accgccagat ttcatctggc aaaagatctt tgggaatcga acttttagtc aattctttga
    27661 catatgtctg gaaataagca tccacatctt ttgaaatgag ggtctctaga tcaatgttct
    27721 ccacaccggt ttcggaaaga gaagtgactt tttcctcaat atcgttatag acctgcatgt
    27781 ttcgttctgg atcttgtgac cagacaacat cagcaaatcc tggttcaaat gtcaaatact
    27841 gacttttgct gtccatgaat cggtcgagac gttccggtat ttccttgatt ttcagtaatc
    27901 ctttttgtac ttgaagggaa agttcttctt ttcgaatgat tagctttttc tcatgatgag
    27961 tacggtaatg taaaaaagat tttgcacaga ctagtttcaa gtctcgtttc acttgaccga
    28021 tatttccttc cgcatcataa agcatgaagg caagaatcgc ttctttttcg atatcgatac
    28081 gttgattcaa acgattcgct tcttgtttga taaaaagaga gatgatttca taacgctcat
    28141 ccagtgaacg tgaagcaaga ttcggcaagg tgattgccat aggaatccgt cgattgaacg
    28201 tagataagaa actttctgaa gattcagtcg tcgccccaat gatctgaaca gatgcttcat
    28261 gtacttgtgc actttctcca agcggacggt aaataccttt atcaatgaaa gtaaacagca
    28321 tttcctgccc ttccggcggt aaacggtgga tctcatctaa aaacaatata ccgccgtctg
    28381 ctttggctat tagaccggga ctatcttgag ctgctcctgt gtaagcccct tttttgatcc
    28441 caaagatgtg tccaaagagc agttgcggat tttgagcata atccgcacaa ttgaaagaaa
    28501 caaatggcgc atctgcagaa agcatctctg aatcgattgc aaaatgatac atacattcag
    28561 caaacatcga cttccctgta ccagtttctc caaaaataat cgtatgcaac ccccgcggtg
    28621 gataaagaat cgcagctttt gcctgctgta tgctgacttt taaagaatca ttttctccaa
    28681 cgagtgtttc aaaagtcact tgcgtagaag agtcgacatg cacttcatca gattttgact
    28741 tttccgttgg caaagagtaa acgactggtc ggccagcggt ttttacaata tgatgggcct
    28801 tgtatagttc acttaaatat cggcttgcat tgcttcgatc gatagttaag cgctcagcta
    28861 catcacttgc tgtcaatcct tgttggtttt ctgtcaatag ctgcaagatc tcttcttgac
    28921 gttctttcac ataacccctc ctctggcaac caattttgtt cccgttttca taatcatagc
    28981 ataaaaaggc gcttatttgc gccttgctcc tttatttctc tctttttttg atttattttt
    29041 aacttttttt cttatttttt ctttgttata ttctgggttc tgctcctttc cagaaaaagt
    29101 tttcgcatac tttttattta ctgcaggagc tgcatcttct ttggttggct gctcagtttg
    29161 cagcttacct ccgtataaaa aaatctcttc aagtgttata tttgatggac gtaaaagccg
    29221 ttttaaatct cttagattat tatcttgtac gagtgtaatt actattcctt cattacccat
    29281 tcgaccagtt cttcccgaac gatgcaaata agcttcttca cttaacggaa cttctgcatt
    29341 cactacataa ggaagacctg taatatctaa acctctagcc gccacatctg tggtcaaaag
    29401 tgctctgacc attttctttt tgaattgttc tagcgctgct tttcggagta gcttgttctg
    29461 atcagaggcc agacttccta ccggtacacc tctatacagc aatttttctt ccattacgcc
    29521 taattcagtc agttgattaa agaaaatcag accatgaaaa tcttctatat gcgtgagacg
    29581 tctcaagtag tcatctttct ttcgtatcgg tacacgtaaa aagtaatgtc tcagtccttt
    29641 tcgagaatcg tcctctgctg tcacgtcaat tgtctcaaac gttttcccag tgattttttc
    29701 aatgctttca agggaacgat ctgctgtcgc tgaaaaaaaa gctagctgat actctgttgg
    29761 agcttgatga agaatttgat caatgatttg gctgttccca gcgtcaaata gctggtctgc
    29821 ttcatcaaat accattgttt tcacttgatg tgcttttatt ttttttgctt tcatcaattc
    29881 caatattctt cctggtgtac caatcaagat ttctggtttt tccttcaaac cctcaatctg
    29941 acgtttgacg ttggctccgc ccaccaaaga ttggactttc aactctttgt cttttcccca
    30001 ttcacgtgca acatctgcta cttgaatagc tagttcttga gaagaagcaa aaatgaccaa
    30061 cgcacttgct tcacctggtt ccacgtttag tagtagaggc caaagataag ccagtgtttt
    30121 acctgaacct gtaggagaaa ccgctactat actttgttta ttacttagtg gctgaaaaac
    30181 tgctcgttga ataagagaag gtgagataaa ccctttatcc tcatattttt tctgccaatt
    30241 ttcgggaaat acttgtacaa attcgttcat taaaaatctt cctcattatc tgcatcgaat
    30301 ttgatgccgc cattttttct taaatcataa atcacttggt tcacatttct agctagctcg
    30361 atccactctt catacaacaa tccccaagct ggatctttcg gatgagccat gacattggca
    30421 aaatcctctg cttcatctgc cattggattt tctgcagctg ttaccgccaa atcaatgatt
    30481 tctttatgcg cgcgatcatg aaattgtgct tgttcaattg cattgactcc attcatcact
    30541 agcgttcctg agtcaaaata aatctctgac ggtaaataag aatcaccgat tttacctggc
    30601 tggatcgata catcaaaatt ctcataacgt aagatccccc atccaagacc atctacacca
    30661 gtagaaatct ttcttgggaa ataatggact tcttttggca ttccaaacca agcaacagct
    30721 gcatatacaa ggtaaacacc taaatccatc aatgcaccac ctgagaaatg agtcgagaag
    30781 atattcggtt cttctccatc taatacttgg tcatagcgcg aagaatattt catatagctg
    30841 aaatttgcac cgattatctc gtcttttaat ggtaagtaat cggcaatctt tttaaaacct
    30901 gattcatgga tatttcttgc tgcttcaaaa aaatacacat tgtgttcatt tgccaaatgg
    30961 atgatttcat ccatttcttt tggcgtcgaa aaagctggct tttcaactat cacatttttt
    31021 cctgccaaaa ttgcttgctt agcttgacta aagtgaagac tattcggaga agcaatataa
    31081 atggtgtcca tatgagtaat attgaaaaaa gtcttcaaat ctgttgcgaa ttcgatatcc
    31141 ccttcataat gagaaccaaa ttcctgtgct ttttccaaat gtctagaata taccgctgtc
    31201 aattcatatt tttttgtgct taaagctgct tggacaaatt gatgagtaat ccaatttgtt
    31261 ccgataatac ctaagttgat cattatttta ccttctttct attgtatagg atcttcaaaa
    31321 gggatatcta catcaaatgg aatggcctgc ttgacagtta cctcatctgc cgatacttga
    31381 caattatagg cgaggatttg aactccgctt tgctgcgcat gtcgaaccat atcagctagt
    31441 gctggttgca tttcttcatg gatcgttgcc agatgaagat gttcgaactg agcaataaac
    31501 aagacataag cggcataacc atcttgtgcg gcatacgtta attctcctat atgtttcaat
    31561 cctctgagtg tgggcgcatc aggaaaagca ccaatcgctt tattttcaag tgtcatacct
    31621 ttgacttcaa caaatgcttt ttgtccgacc accgtctcta aataaatatc aaatttggag
    31681 tgaagataag ttacttcacg cttgtatacc gtaaatggac cagttaatcc tggtaaaagg
    31741 atcgtgccgt ctaaaatacc atcaattgcc agccgattag gcaattgact atcgatatta
    31801 aaccagtcac ccttcttttt tacagcaatc agatcatagg cggttttccg tttaggtcca
    31861 ggagcaaata caatagccac ttcagcgcct ggtatcagga cctcttttcc tctacctgta
    31921 tttttgacat gcgcaataat tatttctccg tcttttttca tacaatgtgc tataaaccga
    31981 ttcgggcggt caataaaaaa agctatttcc acttgctgat acttcaaatt atgtcacctt
    32041 tcttcctact atgtatccta tcataatttg tagaaaaaca gtaaaaaaac cagtcgaaag
    32101 tcttagagaa attttagctt ttattcggta caatgaaata aagaagaaag aatatgtcta
    32161 tgcgattatt ttctcataaa ggagtgattt ggtgataaaa aaaatgatct attttcttgg
    32221 ttcactcatt accgttctct caagttttat ttacttgaaa aagaaaaaac aagcggaaaa
    32281 gtcagaagga ctctcaaagt atcaaggtac atggttcttt agtgatagcc agcaaaaaca
    32341 acaattacag attacatctg attacacgtt tttgatcaac ggtcagtctc ttggcacatc
    32401 tgtggtagaa atagatgaat atcgtttagt cgtacgtgat cagtatggtt tttatcttat
    32461 cttttcttat cccgaacaaa ctctttatga cgaatctgca gacacttcat ataagttgtt
    32521 atctgaaaaa tagatccctc ccaatgtata tatatatcct ttataaacga aagaatgagt
    32581 cttctctatc atctttcgtt ttttgttgta aaaacgtcta aaaatccttt gttttttatt
    32641 tagaaaaata gtcaattaac gttttttttg ttacacttac taatgatggt tttttaccat
    32701 gaaaggatga tcgaatatga caccaaatcg agaagattat ttgaaaatca ttttagaact
    32761 aggtggagat acaacgaaag tcaacaataa acaaatcgta tctagtctag atgtctcagc
    32821 tgcatctgtc agtgaaatga tttctaaact tgtgaaagaa gagcttgtag aacattcccc
    32881 ttatcaaggt gtacaattga ctgcaagcgg actgcaaaaa gctagcagtc tgattcgcaa
    32941 acatcgctta tgggaagttt ttttagttga acatttagac tatagctgga atgaagtcca
    33001 tgatgatgct gaagtattgg aacatgtgac ttcggaacat cttgcagatc atttagaaaa
    33061 ctatttgaat catcctgaac attgtcctca tggaggatcg attcctaaaa aagaggaagt
    33121 tgtccatgaa gaacgccgac aaacattgga gtcctatcct gtcggtacaa aagtgcgtat
    33181 cgcacgagtg cttgatgaaa aagaattgtt ggattactta gtcactattg acctgaatat
    33241 caacgaagaa tatgagatcg ttgacatcgc agcttatgaa ggaccgatca ctatccaaaa
    33301 tgatagaaag aaaatccccg tcagctttaa ggcggccagc acgatttttg tcgataaaat
    33361 ataaaggagt gtatccacat gtctaatgaa aaaaatgtat tgtttacgat attcggcggt
    33421 acaggtgacc tagcccagcg taaattgtac ccgtctttgt tccgccttta cagaaaagga
    33481 aatttaggcg agcattttgc tgtaatcgga accgctcgcc gtccctggag cgatgagcac
    33541 taccgtgaag ttgtcaaaga aacaatccgt gcattgaatc cttcagatga agaagcaact
    33601 acctttgcaa gccattttta ctatcaatcc catgatgtaa atgattcaac gcattatcag
    33661 acattaaaag aattgtctga ccgattagat ggacactatc aattaaacgg taaccgtgtc
    33721 tattatttgg ctatggcacc tcaatttttt ggaacgatcg tttctcattt gaaatcacaa
    33781 aatattatga caaaaaatgg cttcaatcgt ttgatcatag aaaaaccgtt cggttctgat
    33841 taccagtctg cttttgaatt gaaccagcaa atcagagaag tattcccaga acaagatatt
    33901 ttcaggattg atcattattt aggaaaagaa atgatccaaa acatctcagc tatccgtttt
    33961 gccaataata tttttgaggc tcaatggaac aatcgctata tcgacaatat ccaaattact
    34021 tttggcgaaa acctcggtgt agaagatcgc gggggctact acgatcatag tggcgcactt
    34081 aaagacatgg tgcaaaatca tattttacag gttgtagcat tacttgcaat ggaaccgcca
    34141 gtagcatttt ccgaaaaaga aatccgtaca gaaaagatca aagcattgaa agctatccgc
    34201 atttataacg aagaagaagt gttagaaaac ttcgttcgcg ggcaatatgc tcaaggtgaa
    34261 ttggatggtg tacaattcaa gggctatcgc gaagaagaca aagtggatgc tcaatcagag
    34321 actgaaacat tcgtcgcagg caaattcact atcgataact tccgttggtc aggtgttcct
    34381 ttctatgtac gtacaggcaa acgtttgact gaaaaaggga ctcggatcaa catcgtattc
    34441 aaacaagttc ctgttaatgt attcaaaaca tctgtcgatg aaccatgtga cgatacgaca
    34501 cttccaccta atgttctaac gatctatatt cagccaactg aaggattttc tttatccttg
    34561 aatgggaaag aagtagggca aggatttgaa acggaaccaa tcaaattgga atttagaaat
    34621 agtgcagaaa tggttgaaaa tagcccagaa gcttacgaaa aattactatt agatgcgttg
    34681 aatggtgatg gaacgaactt ctctcattgg gaagaagtcg ctcaatcttg gcatatcgtg
    34741 gatgtcattc gaaaagcttg ggaccaaaca caaccggatt ttcccaacta caaagctgga
    34801 tcaatgggac ctaaagcagc tttcgaacta cttgaaaaag acggattcga atggatctgg
    34861 caaccagata actggtatcg tgaacgtgga aaattaaacg attgataaca aaagggggtg
    34921 tgacaaagaa catgtcacac ccccttttcc cttttacttt tcttcgaatc cttcagctac
    34981 agcttcagga tagaagtctt caacgatttt tgcacaacga ccgcatagat gaggaagttt
    35041 ttcatctact cctacatctt tacgaacttg gcgacagcga tcgcaagttt ccccgtctgc
    35101 tttttccact gtaatagcaa catcatcaaa taccatagcg ttatctggca cttgttcatc
    35161 tgctgccata acttcaaagt agtctggtga aacgatcaat aattgtgcaa tatctgcatc
    35221 caaagctttt aacatttcac gaacttgttg ttttggataa acagttagtt ttgcttccaa
    35281 agatttacca atcaatttgc tgtttcttgc ttcttccaat gcttttaaga cattatcacg
    35341 gaagtccatg aaagcagtcc aggtatccat caattcttct tggtttgcaa actcttcata
    35401 accaggaaac tcgcttaact gaacgtactc ctcttcttct ttcaggaatg accagatttc
    35461 ttctgctgta tgcggaatga taggagtcaa taattttgtc aaagctacag ctgtttgata
    35521 gaaaacagtt tgcatgcaac gacgttgata atcatcttct gcttcaatat aaactacatc
    35581 tttagcaaaa tccaggtaga atgacgatag atcaaccgtc aagaagttca taactgtacg
    35641 gtaaatatgc atgaagttat atttgtcgta cccttcttct cggatttctt tgattacttg
    35701 gttcaatcga acggtcatat atttgtctac agaacgtaaa tcattgtacg aaacagtatc
    35761 ttttttagga tcgaaatctt ctgtattcgc taataagaaa cgcatcgtat ttctgatttt
    35821 acgatatact tctgccactt gacttaagat atccatagaa acacgaacat ctgcttcata
    35881 atctacacta gatacccata agcgaaggat atctgcacca aattgtttga tcactttttc
    35941 tggtgcaatg atgtttccta aagatttact catcttacgg ccttcaccat ctaaagcaaa
    36001 tccttgagaa ataactgatt tgtatggtgc tacaccattg atagccacac tagtcgtgat
    36061 acttgagttg aaccagccac gatattggtc tgatccttct aaatacatat ctgctgggaa
    36121 tgataattct ggtcgctgac gtaaaacagc ttcatgagaa gaaccagaat caaaccagac
    36181 atccatgata tctttttctt ttgtaaattc gccattagga gaacctgggt gggtaaatcc
    36241 ttcaggaaga agttctttcg cttctttttc aaaccagata attgatccat gttcagcaaa
    36301 taattttgcc acatgttcga ttgtttctgg tgtgatgatt gcttctccat tttcagcata
    36361 gaaaatcgga agtggtactc cccatgcacg ctgacgggaa attacccaat cgccgcggtc
    36421 acggatcata ttatacaaac gtgttttacc ccatgggata acccactcta ctttttctac
    36481 ttcatccaaa atattctgac ggaatttatc gatagatgca aaccattgtg gagttgcccg
    36541 atagatcaca ggctttttcg tccgccaatc gtgaggataa ctatgagtaa agaaatctag
    36601 cttcagcaaa gctccttttt cttttaatag atcagtgatc ataggatttg ctttgtcata
    36661 aaagatacct tcaaatccag gtgcttcttc tgtaaacaat ccttttgagt ctacaggtga
    36721 cactacagat agtttatatt tgctaccagc gatatagtca tcttccccat gtcctggagc
    36781 agtatggact aatccagtac cagcgtctaa agtaacatga tcgcctaaaa tcacaagtga
    36841 ttcacgttca tagaatggat gacttgcagt catatattcc atttcttgtc cagcaaattc
    36901 ttttagaact gttacatttt cccaaccgat ttctgattgg actgtttcta gcaaatcttt
    36961 cgctacgaca aacttacggc catcggcttc caccaataca tacgtaaatt ttggatttac
    37021 agcaatacct tggtttgctg gcaatgtcca tggtgttgtc gtccaaatga caaaagctgt
    37081 atcttcatca agaatatctt tgccgtcttt gacattgaaa gctacaaaaa tagatggaga
    37141 ttttacatct ttatattcaa tttcagcttc cgctaaagaa gactcacttg acggagacca
    37201 gtaaattggt ttcaatcctt tgtaaatata gcctttttca gccattttac caaatacgcg
    37261 tacttgtgca gcttcataag aaggatctaa tgtaacataa ggatgttccc aatctcctgc
    37321 aactcctagg cgtttgaaat ctgctcgttg cttatctact tgggaaagtg catattcacg
    37381 acatttttcc aagtattctg ccatggacat ttctttacgt ttcacgcctt tattagccaa
    37441 tacttgttca atcggcaatc catgtgtatc ccatcctgga acatatggtg cacgatagcc
    37501 agacattgat tttgaacgaa tgataatgtc tttactgatt ttattcaatg aatgtcctaa
    37561 atggatatta ccattggcat atggaggtcc atcatgaaga acaaagctag gttttccttc
    37621 gttcaactct tgtctttttt ggtagattcg attttcttcc cattcttttt gccactcgcc
    37681 ttctcggttt ggcaaattac cacgcatagg gaattttgtt tttcctaatt gcaaggtttc
    37741 tttcattttc attcaaccat acactccttt atttgttttc cggacacaaa aaagcatgtt
    37801 cagccgcaag ggacgaacat gccgtggtac cacccaaatt tagttgcatc tatctagtgc
    37861 aaactctctg tgaccaataa cgaggtcagc cgctgcttct tactctaatt tcggaaaagc
    37921 aggtctcaag tagaggatct ttcaccatgc agtgtcttac cgaacttcca ctctcatcgg
    37981 ctcgctttaa gaataacaaa gtgcttttct gttctactcg ttttcatttt caccaggcac
    38041 gatcgagata tcaatagcct gtgtatcaaa tgccgtcata ggtgcttcta tgactcctgt
    38101 gtctgagttt acatcagtat cattttcatt gtcaagctgt tttgctaaaa cttctttgat
    38161 cacttcatgg ctgtcagtca cgtagctgga gaatggttgt aaaatttcat cccattccgg
    38221 acttttcact tgttctaatt gagactcaag catcaagctg atacgttgat ggaatactcg
    38281 tgtttttttc ttaagatctt cagtttctgt cgcaagctgt cttgcttttt ctgttgcatc
    38341 tgtcaagatt tttcttgctt tttcttctgc atcagttgtt aattgatgtg cacgtttttc
    38401 agcgttcgca actaactcat ctgctttgtt ttgtgcagaa gtaacgatca cttcggattc
    38461 cttgcttgca cttgttttta ctttatcagc cgtatcttga gctacgatga ttgattggtt
    38521 caaagcatct tttaattcat tgaagtactc aagtttttct tcagaatgtt tgactgcttt
    38581 ttctaattct cgatttcttt gagtcaattc ctcatagtca cgaacaacta aatctaagaa
    38641 atcatctact tcgtcttgat tatatccccg catttttgtc gggaatgtct tattttggat
    38701 atctaatgga gttaaagcca tgagtaaacc acctttttat tttatttacg taatacaccg
    38761 aacagtaccc tgtacttttc ttttttcgtc ttcccctcaa gttcttggat ctggactctg
    38821 ccataacctc tgactgaaat aatatctaat agttcaagta caaaatcagg tcttgtattt
    38881 tctgtccagt tcacttttac tttaccagct tcgatcagct gcttggaccg ctgtctggaa
    38941 atattataaa tctcagagat cacactgtct agtcgcatgg aactaactgt tcctctttct
    39001 tgtgtccagc tatctttcgg cataatcatt tctgtgtatt tgattggttc caagcgtaca
    39061 gcaacctttc caatctttgt tacttgtgca gcaacaaaat gatccacctc atttgcaata
    39121 aatacttgcc agcgatcacc gtccgagatg atatcgccaa agtaagagcg tttgatcccc
    39181 gttcccacta aagttccaag gattttccca tgacttaggc tggcaaattt gctaggataa
    39241 tggatttcat acaatgctgt ttcaaaatct tcctctacag gttcgtaata atcggggaaa
    39301 atcaaacatc tttttctttc tgcctgttca tatccgccat aaaacatgaa acgcaaatca
    39361 ctgctttgtc gaatcagtgt ttctaaaata taggattgtc ttgggtcaag aaattcggtc
    39421 agatagggtg catattgtcc ttgaacctgt tcaatccaat cgcctaccga atcgataaat
    39481 gtacgttcat ctgcccgaaa gtgctggtat acatttgcat ccaaaatttt cccctcctat
    39541 ctattctgag aataaagatt ttgttgcttc aatcaataaa ctaaatggac agttatccat
    39601 ataacgatct gtgctaaacc ttgtacggct aagttcaaga tcaggattgc ccctaaaatt
    39661 gtgaaatcca agggtccaat cctcaaaggc agacgatcga acaaactgat atacggttcg
    39721 cagatcttag ctaataatcg tcctagccct gattgatacc cgcctggaaa ccaagagagc
    39781 aatgcatata ccatcaagat aatgctatag aaataggctg cccgttgaag taaatgaagt
    39841 aatagtgcaa tagtataaat cgtagttcct cctcttacag atcaaacaaa ttcgcatctg
    39901 caaaagattg agcggttgaa ctgtcaatct ccatcccagt cggtgtacaa agaaaaatct
    39961 catctccaac acgtttgata tctccatcta gtgcataaac tgttcctgtt agaaaatcaa
    40021 cgattcttct cgcttgtttt tcttctacta aatgaaaatt cactaaggtt gcttctccag
    40081 caatgattcg tttagcaatc gccatagctt cagaataagc tcttggttcc attacagtga
    40141 tttttccagc aagatttgct ttaggttgtg tagaggttct tgaagctgtt ctttctttcg
    40201 ctacagtttt tgtaggtgat tgatgtagtt caattacttt tttcttttca gccggctgaa
    40261 tactttcatg cgttacagga cgtgaagtcg ccgttttttg tttttcatga gtattcgcag
    40321 cagatacaga atgagttgat tgattctgtg ttgtattttt tttagttggc tgtacagcct
    40381 tttttgttgt ttcaggataa tcttggtatt cgtactcttc ctcatcagct aatccgaaaa
    40441 aattagcaat tgctcctctg tttaaaagcg gcatttttgt tcctccttta ttcgtctttg
    40501 aatagcgctg ttcctacacg gataaatgtt gcaccttctt caatcgcaat ttgaaagtct
    40561 tgactcatcc ccatacttaa ttcactacat ggtgcatggg cataaccttt tgcttgaaca
    40621 agatcacgca attctttcag cgtaccaaac actttatgga tttgctcatc agaagcttct
    40681 ttaggcgcca tagtcatcaa cccaacaacc ttgatttttg tgcaggcagc taaagattcc
    40741 acaaacggta aaagctcatc tggtgaaatt ccatgttttg attcctcacc agaaacattt
    40801 acttcaataa aacaggataa ttctttctct aaccgcttct ggatttcttc tgctaatctt
    40861 aaactgtcca acgcatgaaa gtaatcaatt tcatttacaa tcaattttac ctttctgcgc
    40921 tgtaaattac caattaaatg ccactttatt tcaggaaaat caaataaatc ttgtttcttt
    40981 tctaataatt tatctactcg gttttctgca caattcataa cgccagctct aaccaattct
    41041 tcagcttgag cggattcaac agattttgtt acagctacta atgtaacttc tttcggatca
    41101 cgatgaacaa gagcacacga atcctgaatt tcttgcttga ttttctgcaa gttgtcagca
    41161 atcatgtgct cttcctctcc tttatttttt acgacggaaa aacggtggcg tatttaattc
    41221 atcatcgcca tgagattttg tttcttcgcg gttgaatgta tcaaattctt ttttttcgat
    41281 attgtcaaaa ttggaatcat caactctagg acgaacattt tgttcacgtc tgatatccca
    41341 atcgccaaaa ctgttttctt cttctgaaga agttggttta gcttgatcca tatctaatac
    41401 agtcttttga gcaggtgatt gcatttgtgc ttgtctagct ggtcggctag acttgcgttc
    41461 cttttttgat tcatcgatac ctgttgcgat gaccgtcaca cggatttcat cgcccatttc
    41521 ttcattgata gatgtaccta gaatgatatt tacatcgcca gttgctgcat ttgctacgat
    41581 atctgaagca tcttgtgctt cgaacaatgt catatccaaa ccgcctgtga tgttaagaag
    41641 tacttgttca gctccatcaa tagatgtttc aagcaatgga gaggagattg cttttttcgt
    41701 tgcttctact acgcgatctt ctccactagc cacaccgatc cccatcaatg ctgtaccttg
    41761 gttttccatc actgttttca cgtcagcaaa gtcaaggtta acgtaacctg gcgctgtgat
    41821 caaatcagag ataccttgta caccttgtcg taaaacatta tctgcttcac ggaaagcttc
    41881 aagcatcggt gtctttttat ctacaacttc taataaacgg ttatttgaga taattaataa
    41941 tgtatccaca ttttctttca aacgagcgat accttctgca gcaaaacggc cacgtttcgg
    42001 tccttcaaaa gtgaatggac gtgtcacgac accaacagtc aatgcgccca attctttcgc
    42061 gatacctgca acgattggtg cagcacctgt accggttcct ccacccattc ctgcagtgat
    42121 gaaaatcata tctgcaccgt ctaaagcttc gcgtaaggat tgttcacttt cttctgccgc
    42181 tttttgtcca acttctggct gagaaccagc acctaaacca cgagtatatt tggggcctaa
    42241 ttggataact gtttcagctt ttgaattttt taatgcttgc acgtctgtgt tggctgtaat
    42301 gaattcaaca cctttaacgt tttcttcaat cattcggttt acagcatttc caccgccacc
    42361 accgactccg atgactttga ttactgcgcc gtcattgata ttattatcaa ttgagaattc
    42421 catatcttat tcctcccagg atgaattaat caaaaatatt tgtaaagaaa cccttgattt
    42481 tattttttac attttctccg gactcttttg gttccggctc atcgtagatt gtttcttcag
    42541 gtgcttcata gctctcatat gtgtttgttg tttcttgttc aactaccatt tgacgagctg
    42601 ctgaggtttc acccgtaaca gcaatttttg ccaattgata cacttcactc aagtttgctg
    42661 aataatccac gatacttatt acatttgtaa ataccgggtt tctcagcccc atgtgatttg
    42721 gaacatacaa ttttacattt acgccgaaga tttcttgtgc caagtccaca acaccaggaa
    42781 gactagcagc tccgcctgtc aatacgacgc cacctggcaa ctctaatgct tcgatttggt
    42841 ccaatgcatc ttttgctttg ttgaaaatct gttccattcg agctgaaata atttctgaca
    42901 agtagcgttc atcgactttc actggttctg actgaccaat cacatctact ggaaattctt
    42961 catctggtga tgtccgttct gggtatgcat ccccgtagtt tattttcaac gcttctgcat
    43021 tgttaaatga cgtatttaat acaatagaga tatctttagt gacaaattcg ccaccttctt
    43081 ggtcaagatt ggtaaacttc aattgcttat catgcataac agccgttgtt gtttggccgc
    43141 cacccatatc aatgacgatc gtaccaaaat ctttttctcc gtcagaaaga attgattcag
    43201 tcagcgctaa aggagtaatc accatttcgt tgacgattaa tccggcattt tccacgcatt
    43261 tacgtatgtt atggatgatc gttttaggtc ctgtaaacaa cagaccgtac atttctaatc
    43321 ttacgccgat cattccacga ggatctttga ttccctcgaa cccatcgaca gtaaaatctt
    43381 gtggcaatat tgacacgatt tgacgttcag gaggtattga acgaacaagg gcagctgaag
    43441 ccacatttct gacatcttca tcggtgattt cctttgaatc tccgttaact gcaatcattc
    43501 cctggcaatt ttctacttca agcatgtttg ctggcaaacc gacacttacg cctttgatct
    43561 ggatacctgc tttttcttct gcttgtcgta ctgctcgttg tatcgcttgg accgttttat
    43621 cgatatcaac gataatgcct cggttaatcc cttctgattt tgcgtttcct actccaataa
    43681 tattcatttg gctgtcgata tattcagcca cgacaacttt aacagatgtc gttccaatat
    43741 caaggcctac atacattcct gtttttgcca tgaatgggat tccctcctac ttactattcc
    43801 atatacaagc gaacaactag cgtccgtttt aaaatatact tattaataga tatattcact
    43861 gcttccaatt ctaccataaa ttactggttt ttagaaagta cattcttact ttttaaagtt
    43921 gttttatcaa ttttcattta aacttgacgg agttgtactt ggattatctt ctatttgact
    43981 tgtttcactt gaatcatttg aatcagatga tccgtttgtg ctagacgatc caggtgtttg
    44041 tgctttttgc gtttctgcct cttcacttga ttcgtttgtt tgatccgcat tttcttcact
    44101 ttcagttgtt tcttccgatt ggcccgattc ttccgtttct tgagtgctac cgtaaggatg
    44161 cgagaagata ccaacttcca tgtcgatcac acctttttca tccatatctt tggctacttg
    44221 cgaataatat ttcatttggt tcgcaagatt attgatattt acaataactt gattgccgtc
    44281 attcatattc aattggatca gatttttatt gcttgctctt ggcgtgtact tgatttcaga
    44341 aattgctttt tgtaattctg atgataactg attgtattgc tttgttaatt ctttgatttt
    44401 tgcagagtct ttgaactctt ccaagatagg gagatttttt gtaggatttg ctactttttc
    44461 atctaatact gttccatttt caatcactgg gtagtattga ttgttttttg cgatcaaagc
    44521 aatctcttta tattctgtta catccaaatc gaattcattc atccctttga aatgtatatt
    44581 tgctgtttcg attcttggct gtgctttttt aaggcgatcg atataagtag atcgatgaaa
    44641 atactgtccc caaacatttt ctcctaatga caattttgta tcagagatga tttcttggct
    44701 gttgactgtc ttattgcctg atactgcaac tgcttgtaat ttgctataag gagaaacata
    44761 atagatcaaa aaaatcagcg gtatacccaa tacagtgatg atcaaagcta accgacgaaa
    44821 tagtactttg tgccgttgac tttttaaatt gggcagccgg ttcaaaaaag aaccgttata
    44881 tggtccaccg tttttctttt gcttgatctc ttcaaaaaat tcgatttttt tctcggactg
    44941 gctttcttct tgctgttcct cttgaaccgt tttttcttct tcagaatctg aaaggggtgt
    45001 atctaatgtt atgtcttctt tttttggctc ttggtttttc atctcttgtt ctgccagata
    45061 ttttcggtta gccttttgcc aaggagtcaa attgttttca tcagaagacg gatgagacaa
    45121 tttttttggt tcttttttct tactcactaa gaccaccgcc tttatttttt gattatttgt
    45181 tttactaagt cataaagacg ttttgaagca tcagggattc cttgttcttt agaagctcgg
    45241 cacatttgct tttgcaattc ttcatcaccc atgatttcgt tgactgtctg acttagtgat
    45301 tcacctgtta gttcattatc tgcaatcatt tttgctgctc ctgcgtgaac aagactcatt
    45361 gcatttttag tctgatgatc attcgtaaca tatgggctgg gaaccaacac tgccggtaac
    45421 cccagagctg tgaattctgc gatcgaagtt gcaccagcac gccccaccag cagatcactg
    45481 gaagccatga cctctgccat atttttgata taaggctgga cactaatatt ttgtatttca
    45541 ggatttattt tatttaggat ctcttcataa tatctatctc ctgaagcata caaaacttgg
    45601 taagaagaag gaatatattc acttaaaaaa actgatactg cttgattgat tttaagtgct
    45661 ccttgactac cgccaaaaat caagactgtt ttcttttctg gatccaaatc aaatgttttc
    45721 aaaatatctg attttttagt atctgctact tcttgtgctc gaggattacc tacaagaact
    45781 gctttgtttt caggaaaaaa ggaagccgca tctttaaatg aaagagcgat ccgatccaca
    45841 tatcggctta aaaatttatt ggtaacacct ggtacactgt tttgttcatg gataactgtt
    45901 ggaataccca ttttggctgc agcgtacact accgctccgg aaacatatcc tccagtaccg
    45961 ataacgatat ctggttgaaa atcactgagg attttttttg cttgacggat actcttaaga
    46021 aacaatcgga ccgtcttgat gttatgcatg gaaatttttc gtttaaatcc ttgaatctcc
    46081 aaggtatgga aaggcattcc tgtatcaggg acgattttgt tttctagacc tcgttttgct
    46141 cccacataca tgaattcagc attcggttct tgagtcttta cataattgac aaaggcaaga
    46201 gctggataaa tatgtccccc tgttcctccg cctgtaacca aaattttcat tttttatcgc
    46261 tcgcttctac tttttctatt ttaatcggtt cacagcttcc atgaatctgt ttcctctagt
    46321 ttcaaaattt gggtattgat cccagctggc attcgctggt gataataaaa ctacatcacc
    46381 atctgtactg ttttctaatg ctaaaggaac tgcttgttct acagaatcgg ctgttactat
    46441 tgtctcgatg cctgcttttt ttccagcatc catcaacttt tcttttgttt ggccaaatac
    46501 gaccattgct ttgattcctt ttaatgaagg aattaattca tcaaatgtat ttcctctgtc
    46561 taatccgcca gccaataaca cgaccttaga cgtttcaaat ccacttagag ccattttagt
    46621 cgctaaaata ttggttgctt ttgaatcatt ataaaatttt ctgccttgga tttctcctac
    46681 atattgcgta cgatgtggta cgccatggaa aaaatgcaac gtttctcgta tagcttcatt
    46741 tgatatacca tacaattttg ctacagaaat agcagctagt gcattttcta cattatgctt
    46801 acctggtacg ccaagttcag atatttccat tattttttct tttttatagt atattgagta
    46861 atcgtcagca tacacaccgt cttccaacac ttcttttgtc gagaaaggca gtacgcgtgc
    46921 ttttgttctt tgcgctaatt cttgtagttc tgactgattc cagttaagga tcagataatc
    46981 tttttctgtc atgttttttt gaaggttcca tttagctttg acatattctt ttcttgtacc
    47041 atgataatca atatgtgctt catagatatt ggtaatcacc gctatatgag ggcggaaatc
    47101 tgtgattccc atgagttgga agctagacaa ttccatcaca attttatctt ctgattttgc
    47161 ttcttgcgct acaccacttg ctggataccc aatattaccc gctaaacgtg caattccttg
    47221 atctgctcct gcattcagta gtagtcctgt catcgttgtt gtcgttgttt ttccgttcgt
    47281 tcctgtaatc ccaataattg ggcattccgc tacttcgtaa gctagctcaa cttccgtaat
    47341 taccggaata cccatttctt gggcttttgc tactaaagga tgactgtagg gaatccctgg
    47401 atttttgacc atcaatgaaa attcttcatc caacaattcg attggatgac tacctgttat
    47461 tacttttatc cctaaagaaa gcagctcttg tgcttccgga ttttcgtcaa aaggttttcc
    47521 atcattaacg gtgacaagtg cgcccaattc atgtaataat ttggccgcac ttactccgct
    47581 ttttgcgagt cctaagacaa gcactttctt attttcatat gttgatattt ttttcatttc
    47641 ccgtcatctc ctaccaaacg atccataatg tgatcaacga agtcacaatg ctgatcaacc
    47701 aaaaaactgt atcaatcttc cactccgacc atccgcacat ttcaaagtga tgatgaattg
    47761 gagacatttt aaaaattcgt tttcctgtaa gcttaaagga agtaacttga agcatcacac
    47821 ttgctgtttc cataacatag atcaaaccaa tcaaaagcaa tgtccattct tggtttagca
    47881 ttatagaaat tgccgctaaa agtccgccca acgctaatga accgacatct cccataaaaa
    47941 tcttggcagg ttttctgttg tagacgaaga aacccagtaa acctcctaaa acacttaagc
    48001 aaatgattaa tacatcatac tgatgctgat gccaagcaat tagaccgtaa gctgcaaaag
    48061 agatacttcc taagccagca actaatccat cgatgccatc tgtcagattt actgcatttg
    48121 aaaatccaac aagccagaag agagcgaaaa taccataaat gattcctaaa ggtagatcta
    48181 gtccgaagaa attcaataca cctggataac cttcagaacg gtaaacaaga taaaagatga
    48241 ttccccctag aatctgtcca atcaatttct gcttcgagtt cagtcccatg tttcttttct
    48301 tgaatatttt tataaaatca tcaagaaagc cgattactcc gtataacgct aatacaaaaa
    48361 gcaagatgaa aagagtagga gttagctgtt tttgccaagc tccaacccaa attcctgtca
    48421 aaatagagcc gatcagaaac actaagccgc ccattgtagg agttccggct ttgctattgt
    48481 gccactttgg tccctcctca cgaatcgcct gaccttgctt tttcatttga aaataaccaa
    48541 taaatagcgg cattgcggca attgtcattg cacaactgct gacaatggga atcaatgctt
    48601 gtgtccattc catgctcttt tctctcctat ttctcttctt attcttctaa agtaaaacta
    48661 agtttgtctt cagtgatttt ttcatatgga gcaagttctt gtttgacaca ataaccatct
    48721 ccatcaaaac taacttcaat accgaggatt ttccccagct tcatgatatc tgcttttgac
    48781 catcctgtta cgtcaggcat cagcttatca ccaccagtat ataggatcag tttttctcca
    48841 gagatcaact gatcaccgtt ggcagtagat tgtttttgta cttttttacc attaccgatc
    48901 actacaggtt gcagtccact tttttgagca tcagctgctg caacgtccgc ttcaagatta
    48961 cgataatcgg ctacggatac tttttctgtt tttgtttctg tatctgaatc ttcttcagtt
    49021 tctttaaaat ccattgcccg tttcatcaat ggatttgcga ttttagccaa tgccattcga
    49081 tcatatgtct tagggtgttt cattgttaga tacaagacat agtctggatc ttcagaagga
    49141 accatctcca caatcgaata caggtaggct gtatctcctg tttgatatcc ccctgtatca
    49201 gaagcgattt gggctgttcc gttttttgcc gatacattgt atccaggtac actatataca
    49261 ccataagcac taccgtagtt ttcgctttcg acaacatctc tcatatattc acggactttt
    49321 tctgttgttt cttttgaaaa aggttgccct agaacttctg tttgagttgt tcgctcttct
    49381 ccagtttggg gatccaccac tttgctgata tatcgaggtt gaatcattgt accgttattc
    49441 gcaatagaag tgaaagcttt catcatttgg aaattcgtca cacctaccgc ttgtccataa
    49501 gcactcattg ctcggtcgac aatatttaat gtaggtaaag caccgtttac ttcatcatct
    49561 aatccagaat gagtactttg tccaaatccc agtttttgta aatagttata ccatcgtccg
    49621 cctaatcgct gttccagtat gaccatcccg acgttacttg accatgaaag agcttgacgc
    49681 atggtcagca cacctttttc accaaagtca tggtcattga tcgtagcatc ttctacttgg
    49741 atttttccag attggaaggt ttcattctcg ttgaattctc cttcttcgat agcagctgca
    49801 gtagtgaaaa ctttcatggt tgatcctggt tcataactat cttgtaccaa gaaattacgc
    49861 cagatagcat cttttccagt caatccttcc attgtttcag gattgaaagt aggtcgttgt
    49921 cccattgcca agatttctcc ggtttttgct ttcatcagga ctgctgtcaa ttcttctggc
    49981 tgatattctt catttacttg atccatcagt gtttctaaat aactttgtag tctgctgtct
    50041 aaagtcgtgt agatatcttg tccgtcgaca gctttttctt cttctgccac tgtacctggc
    50101 agaggatttt gaaaattatc tttttgataa ataatcttac catctttacc gctcaagata
    50161 tcattgtaag cagactccaa acctagttta ccaactaagc cattctcatc tttgtctgga
    50221 actgcgtatc caataaaatg agaagaaaag acgccatttg gatacatccg agcctgatgg
    50281 tcgacaaaat aaagaccagc gacaccttct tttttcatgt ccgcttcgat gttttgcttg
    50341 gtttcttggg agatattttt cccatagcta ccaaattcaa cttgatacag ctcttttttt
    50401 gcgccgtcct ctaaaatatt cagtgcatct tttttgtcta tagaaagatt cttatgcagg
    50461 atttctgcta ttttatcaaa attcttttct tcaacataca gttttttatc cccagaggta
    50521 tatgtttttg agagtatcgc tttaattgaa taagaggagg catcttctgc gagcgccaca
    50581 ccgttacgat cataaatcgt tcctcttttt gcttttacta cttcacttcc ttggtacaac
    50641 tgttttgttt tttcagctaa tgaggtacca gcaacatgac ctccaataac gatatacgag
    50701 aaacggacgg caaataaaaa gaacaatcca atactcgtag caaataaaat gatacctact
    50761 tttttgcgat tgttcatcgg atttagattt ttcttttcca taaaccgccg aattttattt
    50821 ttaagactca tttacttcac tttccttaaa ttgtcgtcat taatagacaa tcctttttct
    50881 tctgcgattt gtttaatccg ttcagtacga gaaagttcgt ttttttcttg ttctaaactc
    50941 gttttttctt gctttttatc attgatttct gtttgtattg acgtgatttc tttttcagct
    51001 ccgctgatat ttgtgcgcag cataactgtt aaaacacata aagcaacaat agcaatcgcc
    51061 acgcttgcca cagcaatttt ttccaaagca gaaatctgct ttaatcgttt tgccggtgat
    51121 tgtggaagaa tgatttcttg attcgaagaa gaatcaggca tttgttgttc cggctgatcc
    51181 accaattcag gtaaatcata ttcgtaatct tgcattttct ttaattctgc catcattcta
    51241 ctcctctttc attcttattt tttctgcaat acgtaacttt gcactgcgtg atcgattgtt
    51301 ttctgttagc tcttcctctg aaggaaggat tggtttacga gtaatgactt tcagttctgg
    51361 ttggaattcg tcaggtatga caggaagtcc cggtggtaga tcttgtaccg tactatattc
    51421 tttaaacata cttttgacaa ttctgtcttc caatgaatgg aatgtgatta cgctgattct
    51481 tccattgact ttcaataacc ggattgcctg ttctaaagag gcctcttcgg caccgagttc
    51541 gtcatttact gcaatccgca cagcttgaaa aattcttttt gcaggatgcc cgccttttct
    51601 tcttgcggga gctgggatga cttgtttaat gatttcgaca agctcaccag ttgtttggat
    51661 tggttttgtt tttcgtactc gttcgatttc acgagcaatt tgtttggaaa atttctcctc
    51721 gccatagcgg aagaaaatct ttactaattc atggtaggaa tattcattaa ttacgtgata
    51781 agcggataaa ggagctgact gatccatgcg catatccaac ggtgcatctt gatgataact
    51841 aaatccacgt tctgcttcat ctagctgagg agaggaaacc cctagatcat acaaaatgcc
    51901 gtctacctta tctacatgct cactcaagcg ttcttctaat tctctgaagt tcgctttgat
    51961 aaatgtgacc ataccttgtt cgacataaga agctaaacgc agcttggcgt gatcgattgc
    52021 tttttgatct tggtcaaacg catacagatg ccctttagaa cttagttggc ttaataaata
    52081 ttcactatgt ccggcaccgc cgagtgtaca gtctacataa acgccatccg gtttgatgtt
    52141 caatccatct accgtttctt tcaacatgac tgtatagtgt tgaaatgttt cagtcattag
    52201 tcagcctctt cctattctta tagtccgaaa tcaatcatac tttcagcaat ttcgtcaaag
    52261 ttctcttctg ctgctgcaga aaaatcttgc catcgtgtct catcccaaat ttcaattcgg
    52321 ttagatacac cgatgactac gcaacctttt gtcaaagctg cataatttct taacgtgctg
    52381 ggtatattga ttcgtccttg tttatcaagt tcgcattcag tggcagccga atagaagaat
    52441 cgcacaaaag tacgtgcgtc ttttttagcc aacggcattt cgttcagttt agcttccaga
    52501 ttttcccatt cgctcatcgg atagccgaaa agacagccat ccaatccgcg tgtcagaaca
    52561 aacttttcgc caagttcttc acgaagtttt gagggcacga ttaaacgtcc ttttgcatca
    52621 atattatgtt ggaattcacc catcaacatt gaaagccccc cattcctcca ctaactaaaa
    52681 ttagtctacc acaatccccc actttctacc accttttctg ggaaatccgg gaacaacttt
    52741 ttttattcat aaacattttt ttaaaaatca agctgcaaaa agccttttaa aagacttttt
    52801 acagcttttc tatttttttc ataggtaaga aagtatgttc acaatgatca agacagcgta
    52861 caagacgatt gtcaacagaa aaaccaaacg ccaagtcatc ttgaaataac gcggataaat
    52921 aatttcttca taataatatg cttggaaaac agcaatacaa attcccatta gcaaaacaga
    52981 aataaagaaa aacggtaaga tagaaattgt ccctgtattt ctagacaatg catgcatacc
    53041 gccccacaaa aaaggaatcg ccaaatcagg ggcttttatt ttaaaacgac gatttaacga
    53101 aaataatttt acaagaaatt ggcaaccaaa caaaacgatt accgggtaaa tataccaaaa
    53161 caagataagt ggtgtgaatg tgcccatgaa cgtaaactcc ttttttatcg ttgcttcaag
    53221 tatactgtat atactttaca caactgtttc attttattta aatttatgca gttatattct
    53281 atatcttaca aacacattcc atgtattcca attccctatc cgttataata aaagaaacag
    53341 gcaacaggag gaaaatcatg atcaaatatt ttacattaga taatgagaaa ctgcgtaata
    53401 ccggaaagga aactgaccaa acgatctggt attgtgtaga acgtcctaat caagaagaga
    53461 ttcatcagct gacacatcaa tttgaaattc cgtcagatta catcacttcc atattggacg
    53521 atgcagaaaa ttcccgtaca gaagaattca atcaatcaaa gttgacaaag ccagcgttat
    53581 tattgttaca atatcctttt gctaaaacaa gtcctagcgg ttattttcaa gtggatacat
    53641 acccgctctc tattattttg acgcccaaaa agaagcttat cacaattacg aatcatgaac
    53701 cactgttttt aaagaaagtc tttagccaga cctttccaaa aagtgatatt tcgcctaatc
    53761 tcaatatctt ctttcaattc atttggcagg tcacactctc ttacaacagt tatttagcta
    53821 cgcttcaatc tcaatgcgat actttggaag ccaaacttca agtatccact gaaaacaaac
    53881 aactttatca aatcatggat atccaaaaaa gcctcgttta tttcagagaa gcgacttctg
    53941 ctaatttaga tgcattgaaa gaactagcgc aaaataaact tttccaagaa aatcatgctt
    54001 tgaaaaatca tttacgtgat gtattggtgg aaacagagca ggcaatgact acttctcgca
    54061 ttcaattaaa actagtagaa caaatgaatc agactttttc agcaatcgta tcgaataatc
    54121 tgaataatat tatgaaaatt ttaacttctt taacgattat tttaactata ccgacaatcg
    54181 tcggcagtat ctatggaatg aacattaagt tacctattgc tgaaagggat gatgccttta
    54241 tatggctagt tctattaaca ttatttattt gtatcgtaac tacctattat ttaaaaaaag
    54301 ggaagttttt ataaaaaagc cctcttgtgg aaatggaaaa aagattgtac acttgaatca
    54361 actaattaat gacgaggttg aaaacaatgc gtgaaaaaaa acaaacaagt acttttgcta
    54421 aagtaacaaa atttgtcatt tggacaatgc tgattttaac gattggatct gttgtattga
    54481 ctgctgtaat gagtgtcatg taaaaataaa atatagctat cagctgctga aaaggacctg
    54541 aaccactgcg aggtggctca ggtccttttt gcgagtttca atagttcttt tgcatgttcg
    54601 agtgtgagtt ttgtaatctc acttccagcc agcatacgtg caatttcttc ttctctttct
    54661 tttgttttca actctgaaac agaagtttgc gtacgtccat caatcactgc tttcacaata
    54721 taatattgat agtcggcgac tgcagcaact tgaggcagat gagtgatgca caaaacttga
    54781 gagtatttcg atatttgaga aattttatct gcgatggcct gtgcaactct tccgctcaca
    54841 cctgtatcca cttcatcaaa aataatactt gtcacacctt gctcagaaga aaaaatagtt
    54901 ttcaaagcaa gcaacatccg agacagttcg ccaccagaag cgactttcac caaaggcttc
    54961 agtggctctc ctggattggt cgtaatgtaa aattcgattt ggtcaaatcc atcatgattc
    55021 aattgtctat tttcttcttt taagaaacgt acctcaaact cagtgttttc catatacaga
    55081 cttttcaatt catgcagaat ggatttttcc aattcttttg cgattttttt acgcgcttca
    55141 tgaagatctg cggcttgttg ctgaagtaga agctcttttt gctctagttc ctctttcatt
    55201 ttgtccaatt gaccttctga aaaatcagaa gaagctagct cttcggtgat ttcgtcatag
    55261 taagccaata ttgactcaat ggattcgccg tatttacgtt tcaactgtcg gattaattct
    55321 aaacgttgcg taacttcttc taaacgactc tcatctaact ccaataaatc aatctgacga
    55381 ctcatatcgc cgattgcatc ttgcagcaaa tagtaagcac tttggatatt ttcataaatt
    55441 gcttcatact ccacatctag atgggcgatc ccttgcagtt ctgaaacggc caagccaaca
    55501 ccatctacac tgttttgttc attattcgtc agtgctccgt aacctgcagc taatccatca
    55561 gcaatttttt gataattgct aagtttttct ctttcttctt ttaatttctc ttcttctccg
    55621 acttgcaaat cagcagcagc tatttcttct tgctggaaat ggagcatatc tattctctga
    55681 acataggatt tttcattttg ctgcactttt cgtacccttt tttccagctc acgataagag
    55741 atatattctt gctgataact ctttttcttc tgttggaaag ctacatctcc aaaacgatcc
    55801 aataaagcaa gatgtgattc aggctggagc agttcctgat gttcgttttg tccttggata
    55861 tctactaaat aactgccgat tcttctcaga ttagctaatg tgataatatg accattcact
    55921 cgacaaacat tttttccagt taaggacata tcccgtcgaa caatcagatt gtcctcatct
    55981 gtttcgatcc ctaattcaac catcagttca gagaatcctt cttgttttgg tagttcaaac
    56041 aacccttcaa gcacacattt ttctgctcct tgacgtatat aatcgcttga acctcttcct
    56101 cctgctaaca gacctaaagc atcgataata atggatttac ccgcaccagt ctctccggtc
    56161 agcgcggtca ttccttcgtg aaaagaaagc cgcaattccg gaataatcgc aaagttagta
    56221 atactgattt cttgtagcat cacattcgct cctcaactta aaggtaatgc aggaagtctt
    56281 tcttcaataa aactgcctgg tcttcagttc gtgtaatcat caaaacatta tcgtcatcat
    56341 tgataatcga aaataattct ttcttgtaac gtttatcaat caaatttgct gctgccgaag
    56401 catttcccgg caatgttttc aaaataacaa atttttccat ttggtcaact gccacgaaag
    56461 cgtcttttaa tagtttttcc aatttagtat ttacatcttc gtttgtttca gctggtatgc
    56521 tataacggta gccgccatct gaagccggta ccttgatcag tttcagttcc tttatatctc
    56581 tggaaatcgt agcttgagta acagatattc ctttgctttt caggatttca acaaattctt
    56641 cctgctttcg gatatcttgc tcactcaaca atcgagtaat caaacgatgc cgatcttttt
    56701 ttctcattac gtcttcgctc cttagcatat tcttcttaat caatgatagc ttatttcctt
    56761 ggtataataa agaagaatcc gcatacttct cacttgattt tcacagttac aatttattta
    56821 tggtcaaact gttcgtgtgc atttgaaacc acttctgcca tattaatagc tgaattgatt
    56881 gttccgcttt cgggtacttt cctcaagtga gccaagaact cgatatttcc ttctcccccc
    56941 gtgatcggag aataacttaa ttccagcaca tcatatccac cttgtgccgc aaatgacaaa
    57001 atgtcttcta aaactttttg atgggtctga gggtcacgaa caattccgtt tttccctaca
    57061 gcttctctgc ctgcttcgaa ttggggtttg attaaagcaa tcacttctcc ctcttctttt
    57121 aaaatagcat gtaatggagg cagcatcagt tttagtgaaa taaaagaaac atcgataaca
    57181 gcaacttctg gaattcctaa ggtaaagtct tcgggttttg cataacgaaa attcactctt
    57241 tccattacga cgactctagg atcttgtctt attttccaag ctagctgatt gtagccaacg
    57301 tctaaagcat aactcatttt tgcgccattt tgcaaagctg catcagtaaa tccgccagtg
    57361 gatgcaccaa tatctaacaa tgtttttcct tcaacagata gatcaaatgc tttgagtgct
    57421 ttttctaatt ttaatccccc tcgagaaaca taaggaagtt ttttaccttt gactcttaat
    57481 tcactactca ctggaatttt ttctcccggt ttatcaaacc gctcgttttt ttcgttgtaa
    57541 atcaaaccag ccatcactga acgtttggct tgttctcttg tttcaaataa tccttgtttg
    57601 accgctaata catctacgcg ttctttttcc attctttccc tcatttctcc acatcaaatt
    57661 gcttgatcat ctggcgcatc agcccgccat caaaagaaaa aacgtcctct tctaatttgt
    57721 ctatgatctt gttggcggat aacagctcaa tctccaacgc ctcacgtgtc ttttctaggc
    57781 ctaataaacg cgggtaagta tttttttcca tacgttcatc tttaccagct gttttcccta
    57841 atgttttcgt tgtaccaatc acatcgagta gatcatctct tatctgaaaa gctaaaccta
    57901 aatgttcggc taatcgttga agtagcaata agatttcttc aggttgcttc gctaatatgc
    57961 caccagccag caaggcataa cgaatcaaat gccctgtctt tctttcatga ataaatgcta
    58021 attcttctaa agaaagcttt ttcgattcgc cctcgatatc tgcagcttgc ccagcaacca
    58081 taccttgtga tcccgcacaa acagctaatt gctgaagcag cagaagctta ggagaattac
    58141 ctaagtgtgc catactgatt agctggaatg ctcctgtaag caaaccatct ccggccaata
    58201 tagctaatgc ttcgccgaat actttatgat tggtaggttt tcctctacga agatcatcat
    58261 tgtccatcgc aggcaaatca tcgtggatca aggaatacgt atgcaccatt tctaaagcag
    58321 ctgctacttg atagacggga acatcaatag gctcatcaaa ggaagcaacg gccgttaaaa
    58381 gtaataaggg acgtattctt ttcccgcctg ctttcactga ataactcatc gcatcataga
    58441 gtcctctatc cgttgtatgc tcatctaaaa aagacaagat ttctttttct acagcaggta
    58501 gatgaatagc tgaaaagtca gttaatctca tctcagcttt cctcgtttcc gtcaaaatca
    58561 acttcttcat ttgattctgt catcattttc gtcaaggtct cttctgcttt agctaatgta
    58621 tcttggcaat gtttgcttaa ttccattcct cgtttaaacg aatctagagc tgattctaat
    58681 ggaacgtctc cttgttctaa ctgcatgaca attttttcta attcttgcag tgactcttca
    58741 aaagttggat tttccatatt tttattctcc tttgtctatc tcttgtactt gtcctttaac
    58801 gattccatct tgataatgga tcgtcagcaa atccccttga tgaatctcgg tgacactttt
    58861 tactacttga tcatttaacg tcgtatagct gtagcctctt cccatgattt tcaatggact
    58921 caacaaatca agagattgaa cagcttgagc aaaacgctgc tgctgttgtt tcatatattg
    58981 ttggattttc tcttctaagc gtttttctag ataagcagtt tcttggcgag ctgctttaac
    59041 tcgataaagc ggagccactt gttccaattg gtggcttaat gcgatcgttt gtttttcttt
    59101 cgcatgataa atttgctgtg tagcttgata taatcgctgt ttcaactggt ctaatttgat
    59161 ggtctgacct tcatacaatc tttcaggctg ccgaaataca taggaacgtc gagcacgctc
    59221 gaaccgttct tgtttgcgct ggatctggcg tacaaacgcc tgctgcaatc tagcttgccg
    59281 ttcttgaatg cgcatcaact cttctgacaa aacaggaaca gctaattctg cagctgctgt
    59341 tggagtggca gcccgaacat ctgctactaa atcagcgatc gtggtatctg tttcatgtcc
    59401 tacagatgaa atcaccggcg tattagcttg ataaatcgcc cgagcaaccc gctcttcatt
    59461 aaacggccat aaatcctcaa tcgaaccacc cccacgaccg atgatcattg tatcgaaatt
    59521 gcccatctct tctactctct ggatattcct gacaacattg tccgctgctt ggtttccttg
    59581 tacaactgtt gggaacaaaa ccaattggac aattggatat cttcttttca ccgtcgtgat
    59641 gatatcgcgg ataaccgctc cgcttggact ggtaaggacc gcgattcgct ttgggaaacg
    59701 tggcagcatt tttttaggcc cttgaaaaag gccttccttc atcagctttt cttttcgttc
    59761 ttccaattct tgatataaag cacctattcc atcaggctcc atgtgctcta catagatttg
    59821 ataagaacct cccgcttcat ataatgaaat acgcccaacg acaaggactt tcattccttc
    59881 ttttggctgg aatcgtaatt tttggaatgc tcctttgaac ataatagcag atatttttgc
    59941 atggtcatct tttaagctga aatattgatg ggcattggca cgtaaacgga aattcgatat
    60001 ttcgcctgtt aaatatacgc gttctaaata aggatcggca tcaaattttc ttttcaaata
    60061 cttcgttaat gtagtgacag ttaaatattc ttgtgccatc tattcacctg ctccaaaagc
    60121 ttcggcagat tttacggttt gatacatcag catcgtgata gtcattggcc ctacaccctt
    60181 tggcacagga gtgatccagc ctgcaatagg ctccacttca tcaaatttca catcaccgat
    60241 cagcttaccg ttagcatcac gattcatccc tacatctatc actacagcac ctggttttac
    60301 gaattctttt gtgacaaaat gtccacgtcc tattgctacg actaaaatat ctgcttcttg
    60361 tgccagttca gccagatttt cggttttaga atgagcaaca gtaacagttg catctgccat
    60421 caagagtagc tgagccatcg gtttaccgac gatattgctt cgtccgataa ctactgctcg
    60481 tttacccgcc ggatcgatag aataagcttc aaacattttc atgatgccgt atggagtaca
    60541 aggaatcttg tccggtttac ccgcaaggag tctgcctaaa ttcatcggat gaaaaccgtc
    60601 tacgtctttt tcagggcgga tagctaacag gatcttttct tcatctatat gctttggcaa
    60661 tggtaattgg accaatatac catgaaaact aggatcttga ttgtattttt caatttctgc
    60721 aagtagttcg tcttcagaaa tcgtctcagg ataacgctct accaatgaat ggattccgat
    60781 ttcttttgct acacgttctt tatttcttac atagatttgg ctggctggat tttcccctac
    60841 caataaaacg actaatccgg gatggatacc attatctttc aatttttgga ttttttcagc
    60901 aatctctgcc tgcatttttt cagctaattc ttttccgtta atcaattctg ccatctaacc
    60961 actcccagat attttttgtg ttttcattct accatagaat tttcccgatt tataacaggt
    61021 ttatgatgat tcatagaaaa aagctaggac ttacgcccca gcttctaatt ctttcaatac
    61081 attggataaa acaccgttga cgaatttacg tgaacggtca tcactaaatg tttttgccaa
    61141 ctcgattgct tcatttaaag caacagccgc tggtacatct gtcacataag tcatttcaaa
    61201 aatcgcgata cgcaagatca ccaaatccat ttttgagatc cgattgatgt gccaattgtt
    61261 ttttaaatgt ttttcaatca ctttatccaa ttcatctttt tttgcacaga caccgccaac
    61321 tagcgtatca agataaacag gaacaaactc tgattcatct tcgttgatca tgtcgcggtg
    61381 gtctaattcg attgcgttga aaatcgcatc ttcttttgtt aggtctgcat tgaagtctaa
    61441 tggaaataaa gcctgcaatg ccatttcccg aatctcgtgt cttgataatt ctttattcat
    61501 tctcttcctc ttcgtcttca aaaagttctt cgattgttgg ttgttctaat ttttcaggaa
    61561 caacagctac tacatggata ttgacttcag ataaatcgat atccgtcatg aaaaggactt
    61621 gctggctgac ccgttcctgc atttccattg ctactttagg aactgaaaca ccatattcta
    61681 gataacagta aagatctact ttcaagccat cttcctcgtt tgctaagtaa acaccttttc
    61741 cgtgagctgc acgtcctaat aattcagtga cattgctggc aaaagttccg cgcattccgt
    61801 atacgccttc tactttagaa gctgcaatcc cgataattac ttcgatgact tcaggagcga
    61861 tcacgatctc tcctaaatct tggtttgcgt ttaaaatcaa atttttttca tctgccattt
    61921 atttattcct ccctttgcct aagcacgaga tacatccgaa ttctctatga tgcggcatag
    61981 tgatcacgtg gtaagaaaac tgccgccgat cagctgcaaa ctgctatgaa ttggagaaaa
    62041 cacccaactt tattatctct tacagcttaa aagttcttct atagtgtatc attaaatcga
    62101 gtgattttct ataacaaagt ctgtgaacac tgatttttac gcttacttct tctattgatg
    62161 aaactataag atgatcaatt cttttggtga acgagtcaac acacgatttc catcttctgt
    62221 gatcaacaag tcatcctcaa tacgtactcc accgattcct ggtaagtaga tccctggttc
    62281 atcagtgatc acatttcctg gtacaaattg cttttctgca cgagaagaaa cgtttggtcc
    62341 ttcgtggatc tctaaaccga tcccatgtcc tgtagaatgt ccaaatgctt ccccgtaacc
    62401 atgttttgaa atataatctc tagcaactgc atctaattgg acaccagtca ttcctggttt
    62461 tgcagcatca ataactgcta attgagcatc taagactatt tgataaattt cttttaattt
    62521 ttccccagga tcaccaatag cgaatgtacg tgtcatatcg gaaacgtacc cttcgtaata
    62581 gcaaccaaag tccaatgtga tcaagtcacc ttgttcgatg atttttttgc ttgctacacc
    62641 atgcggcata gccgaacgca atccgctagc aacgatcgta tcaaaagaaa cacctgaagc
    62701 gccaagagaa cgcatataaa agtctagttg atttgcgact tcaatctcag tcatgcccgg
    62761 ttggatcatt ttcaggatgt gatcatatgc catatccgca atatgacatg ctttttcgat
    62821 aatagcgatt tcttcttcat ctttcacttc tctgagttct tcgatcatct gtgaaacagg
    62881 tactaattcc gcatcgatga tttcttctaa aaccgaatat tctaaaaatg agactgttgt
    62941 ttcctcaaaa gctaatgagt gcagttcttc tttgcgtact aagtttgcta cctcatcaaa
    63001 aataggtccg acatttttaa taatttcaaa cccttgagcc tgcgctgccg cctgctcggt
    63061 ataacgaaaa tcagtaataa aaaatgcttt ttccaaagta ataacagcta atcctgtcgt
    63121 acctgtaaag ttcgttaagt aacgaagatt gtacggactg gtaaccaaaa atgaatccaa
    63181 attttcttct tgcatttttt tgcgcaattt ttctacacgt agcatcataa tgaaactccc
    63241 tctttctttg cacgcttttt tccattatag caaacttttc ttcgtacacg acagttaaat
    63301 aaaaatttcc ggaaaacttc tttttatttt aaataaataa agccaaaaag gagtcacagt
    63361 ctcgctgcaa ctccttttag tttactattt tgtaaaaact tatgatcgtt ttccaaagaa
    63421 aaatgagaca actgctacca aaatcactgc gcctaaaata gatggaataa gtgccatacc
    63481 tgcagctttt ggtccccatt cgccgaaaat cgcttgtcca atacttgaac cgactagtcc
    63541 agcaatgata ttcgttacac agcccatcga atttccttta ctggtgatcg cacctgcaat
    63601 tgctccgatc acggctccaa caatcaatgt ccataaccaa ctcataatat aacctccttc
    63661 tttgctaatc aattttagca tatgggattt aaaggggaca aataattggt tttgctattt
    63721 catattttct tctgcaaagt aaaaaaataa agaaaacctc ctcttagaag ttttctttat
    63781 tttttacttg cgtttaaaca tactacgaat accacgtttc actacatcaa agaaactcaa
    63841 tgattgtacc attctatctt catctacata ttctctgatc gcacgaagca cttttttagg
    63901 ttctttggaa gaaaatgtgt atgtaccatt ttttttcgtt tgaatggcat atcgaggaat
    63961 ccattttccc tttagcatga ctgatgctag tacataatcc acttcttccc atggaatttg
    64021 gatatatttg cgtctatctc gcgcatggta aaactcaaaa ccattattac ctatcatgat
    64081 ttgcccgtaa tctgtcagac ccgtaaacga agtagcatca attaccaagt ctacttttgt
    64141 attgattgat tgaaccattt tctccctcca gatatttgtt tatgaacagt atactatata
    64201 aaaaaagctc gggacaacgc ccgagctata tttttatgga ttataaaagt ccgatgacgt
    64261 gtccaacaac accgactacg aataaaccaa ggatgataac gattggagaa acctttttct
    64321 taagcaacca catacataac aatgttaatc ctaatgcagc taaaccagga atcaattggt
    64381 ctaagttgtc ttgtaatgtt gttacctttt caggagaaag ggctaagcca ttattaactt
    64441 gttcaaatgc actcttgatt ccttcaccac ctgaaggcaa tttatcccat tcgatgtaag
    64501 cacctttatc taattttaca gttgatacaa ctggtaagaa tttgattgat acccatcttt
    64561 gtacaagggc agctaatacg aacatcccta aaattgaagc accttttgtt acgtcttgta
    64621 acaatccgcc agaaaggtca tcagtaattt tagaaccagc tttgtagcca aattcttgtg
    64681 tataccacat gaatgcccaa cgaataatgt tccaacctaa gaagaagatg attggtccaa
    64741 ggatatttcc tcccatagct aatgaagcac ctagtgcacc tagcattgga cgtactgtaa
    64801 accagaacac tgggtcacca acaccagcta aaggacccat catacctact ttaacccctt
    64861 gaatcgctac atcatcaaca ggcgcgccat tcgcacgttc ttcttccaga gccaatgtaa
    64921 cccctaaaat tggagaagca atgtatgggt gtgtattaaa gaattccaag tgacgtttta
    64981 atgctgcaga acgttcttct tttgttttgt ataatttttt gattgctgga atcatagaaa
    65041 atgcccagcc accgttttgc atacgttcgt agttccatga accttgaatg aaggtagagc
    65101 gccacgcaac agctaaacga tctttttttg ataatttgat tttttcttct gccattttat
    65161 tttcctcctt caagattaat agtcattcaa aatgtcgcct aaagggtcac cagagttgct
    65221 tccgcctcca ttatttgatg aaccacccat tttagaaagg ttcaagtaaa taagagctaa
    65281 tgcaacacct aaagcaccta gagcaatcaa tgttaattga gaaattgcag ctacaacgaa
    65341 accaataacg aagaatggcc atacttcttt tgtagccatc atgttgatta ccaaagcgta
    65401 acctacagct acgaccattc ccccaccgat agccatacca tctgttaacc atgctggcat
    65461 tgattcaagg aatgattgca cagtttgtgc tgggataaat agcaacgctg ctgctgggac
    65521 cgcgatacga accccttgta aacatacagc aatgatgtgc cacatttcaa ccttacggat
    65581 gtttccttct tcagcagccg catccatcaa atgaacgatt ggaacagcga ttgtacgtac
    65641 gatcattgtt aagaacaacc ctgcaacagc caaaggaacg gcaatcgcga tcgcagaagg
    65701 aacaccttta acaccttgtc cacctaaaac taaaataatt gctgatgcta ctgatgctaa
    65761 tgcagcatct ggtgctacag cggcaccgat atttgcccaa cctaaagcga tcatctgaag
    65821 cgtaccgcca agtacgatac cagcttcgag gttaccggta actaaaccga tcaatgtaca
    65881 tgccactagt ggttgatgaa attgaaattc atcaagaata ccttccatac cagctagaaa
    65941 ggcaactaat acgactaaaa ttattgaaat aatagacatg ataagcctcc tataaatttt
    66001 aatattattt attacgcttt tgctaattca gcttttgctt ttttcaaaat atcatccatg
    66061 ttatcgcgtg aatcattagg tactttacgt acatcgaatt tcacaccttt ttgttcaagc
    66121 ttctcaaacg cttcaacgtc ttcttgtccc atagacaata ctttacttac aacgacttta
    66181 ccgaccgaat gcgccattga cccaacattt aattctttga tgtctacgcc accttcaaca
    66241 gctgtcaata cgtcttcagg attttcaaat aaaagcaatg ctttggtatt tccaaaacga
    66301 gggtcttttg ctacttcgat cattttgcta ataggaataa catttgcttt tacacctgga
    66361 ggagcagctt gttcgattag tctcttacgc agatcatcct ttgatactgc atctgaaaca
    66421 acgataatac ggtttggttg cactgctttt gtccaagctg tcgcaacttg tccatgtaac
    66481 agacgtgagt caacacgtgc taatacatat ttgatttttc cgtctccgac cacagttcct
    66541 tctggaagtg ctcctttagg ttgtgcgtct tcaactgctg ctttcggtgc ttctgccggt
    66601 tctaattctt ccggttttac ttttacaccg tcttttgctg tttcaatgat gtgtgctgca
    66661 atttcatgag cagattccat tgaaaagcgt gatgcgtagg cttcaatcaa cattggtaaa
    66721 ttcaaaccgc tgacgattgc ccatttatct ttgtgctctt cgaataatgt gtttgattga
    66781 ttgaacggtg ttccgcccca taaatcaact aagaataaca cttcgtcttg attatcaaaa
    66841 gaagcaaccg cttcttccaa tttggctctc aagtcatctg gaccttcact tggtttcaaa
    66901 atcacggctt tgacgttttc ttgttcgcca aagatcattg aaccggattg taagattcct
    66961 tcagcaaatt gtccatgact tgctaggata atccctacca tatttatacc tcctatttac
    67021 ttttgttgta acaaattcaa aaatattaat aattgctaac cattacaaca gaattatttg
    67081 accttttcca acaaccattc ttgctacacc tttttgattt tcgctagtag ttccatcaaa
    67141 aggattttcc tgtcagcagg tacttttcga atctctaact ccacttcttg actgttcaag
    67201 taacgaaagg aatcaatatc ttgttgattt actgaaacaa aattggtaat cattgatttg
    67261 ccttcagtaa aactcatacc gccgatattc aacgacttca actgtacacc gcagcggaca
    67321 accgcttcaa catcttgggg attggtgaat aaaagcatca cttttgctcg tgataataat
    67381 ccactatgat agatttgaat cattttctgc acggttatta catttgctgt gatccccggc
    67441 ggtgcagctt cttttagtag aaactttctc aatttatctt gagcgactac atcgctgatc
    67501 acaatgatac gttcaacacc tgtttgcttc gaccaaactg ttgcgacttg cccgtggatt
    67561 aaacgatcat ctatacgtgc caatcgaata tccatctgca aaactccttc ttcctcacta
    67621 cttcacagaa acccctatca tttctgtgtg aacaaccacc tcctagcaca cactactata
    67681 agcaaaaagc gtgccaactt ttttacgata cactaattga atttaaagga ttcttaacca
    67741 atacacaaaa acgatacact ataaaagtgt atcgtttttg tgtattcatg gcacagtgta
    67801 ttctttttta tctaactgat tatggactag acgtagcaga taatacattt cttctatcgg
    67861 cacttttact tttatctgat cttccaacag aacaactgag gctgccaatt ggctataaag
    67921 aggatcattc gttgtatttg tcaattcgtt ttcttccaca gtcaatggtt cattccgaat
    67981 aatacgttcg atcattcctg ctgtgtgcat cactaggttg attttgaatc catattcttc
    68041 atcgccgatt cccgtcgtct gagagatttg tgtagaaaat tgccacattg gatcgattaa
    68101 cttactacca ttaataaaga tgaagttatc agagataaac tcaacacacg tatttttagc
    68161 tgactcttca tctagggaga tgaattcagt ttcctccaat tctgattcca tcagcaactg
    68221 atccaaaatg acttcgatat tttgatcaat aaaacgttct aaaggaataa atggggcttg
    68281 gatattcaaa tcagtaatcc cagtagtagc tatgatctga tattcttttt ggataactgg
    68341 cagttcttct ttcacttcca caatcgacaa agctagaact tctaattctg tttcttgacg
    68401 cttgtttacc gctcgttcga tcaattcttt gattctttgt gctgtacctt cgccagatgc
    68461 gcagatagcc aaaacagctt ttttcttatg aataggtttt gcttcctgaa cagattctac
    68521 agaagatatt tctgcgtaac ctcgaaaatt tttcaatgat tcatgcaaca tatctagatc
    68581 tgtccctaat actgagacct ttcgtacagt ttccaaaact agagaagttg ttaccatatc
    68641 aacagtacgt actggaattc cagtgtctct ttgaatttgc gaattaaacg acgcaaggga
    68701 ccccatatct actaagagta tgaccccgct tccttcatct acttcttgta cattgcgttc
    68761 aattcggtcc aatgctgttt tcggatccat atccaaaggc atgtctactg ctcgtacgtt
    68821 ctctgcatct aacaactgtt ggacaacttg gaccatactg cttgctgtgc tgtttccgtg
    68881 agctgcaacg actacaccga ttctgccgct tgcttgattt gcccgtaacg agacgagtag
    68941 aacggtcaag taatagtctt cactttctgg aatctccact tgaaaatacg tttcgatata
    69001 actacgcact tctttcgcaa cagcatattc atctggaaaa tcaaaagcca tctcacggat
    69061 gttatcattc gtatgccgtt cttctcctaa gttgattttt ttcaaaaaag aactgatatg
    69121 caagctcata gcataaataa agttttgctg aaactcatat ggtaaagatc ttttgaccat
    69181 tacataaatc tgattggtca cttctatgat tttcggatct acaaattctg ctaatttgct
    69241 gtctgcatta aaagaaaaac catggtcttt atagaaagac ttcagatgga tgttgatatc
    69301 tgtagaaatg aaatgattga ttgcttcttg atccaaacca tccgacttca gtaaagctgc
    69361 tttatctccg atgatatcat atagattata aggtaactca taggagtccg attggatttt
    69421 catgatcgta tcatttggtg ctacagttat ctttggctcc aatattttag aaagctctgc
    69481 cattgcactg cggttgcttg ctaattgaat caatccgcta cgaatccctt ctgtcaaatc
    69541 atcgattgtg atcgagattt cgggtgattg catatgattt agaaatcctc gtgcacatac
    69601 taactggata tttgacttca attgtcctat atttccatag gtcacactgc cgatcaatgc
    69661 ttttgctaca tcttctgtca aagaaatctt tcgctgcgtg cggtttgctt cgtgggcaat
    69721 catgattttt actaaatcga ctttctcatt tgcaggacgt ttttcaaaag ccggaagttt
    69781 gatattgatt gggatacgcc gaacaaaagt ttctaataga gaagaacctg ggtcttctgt
    69841 ggtcgcacct acgatccgta catttgcttc atgggatttc gttgtttccc caagacgact
    69901 gtaagttcca tgatccataa agtaaaagat catttcttga ccttcaggcg gcaaccggtg
    69961 aatctcatcc aaaaacagca taccgccatc cgcttgatcg ataatcccca ttttttcttc
    70021 atctgcgcct gtaaaagcac cttttgcgta cccaaataaa tgactcatca aaagctcagg
    70081 gttattggca taatcagcac aattgaaaac aatcagttcc gtttcctcat ctattacatg
    70141 atgcgctttt gcaaaatgat acatcgcgtg tgcaaaataa gtctttcctg atccggtagg
    70201 tcctgtaatc aaacaattta atcctcttgg cggatacaaa atcgcagctt ttgcctgttc
    70261 cactgaattt ttcatgctgc cgttagctcc aatgattttt gcaaagatat ccttagtatc
    70321 tatttggagt gtattttttt cttcagagac cggcgtttct ttgtaactga ctacatgttt
    70381 tgtaactggc tttacgtgcg taacgagtgt ttgatatatg taacgtactg gtcgtccgtc
    70441 tgtctttatc agttttcctt cgcgcactag ctgattcaga tctttactgc tatttgttcg
    70501 ctgaatccct aacacttcag caatttcctg tgttgttact ccttgatcat attctgcctt
    70561 tgttaaatga gcggtttttt ctttgacata catataaatt cggtcaatgc gttttgccaa
    70621 tggtatcgct ccttttctat agactattgt acttaggctt caaaacataa tactatgtat
    70681 ttatttctgt ttcgtcaact atttacctga ttttgttttg ttgataacac gcggatagat
    70741 agaaatgttc aatttaaaag aacgaaaaat cccgatacga tttttataat ggaaacaaga
    70801 aaaagaacac tgtttaccat ataattattt cactgatagc aaacaatata gcttccatca
    70861 attgaaaacg atagttatca aaacatcctg attccaaatt ggcaaaaaaa tattaagtgt
    70921 aaaaagacaa agtaaacttc gtagcctcca ctacaacttt tatttttata aacaaaaaag
    70981 gctgtgacaa aagtcttgtc acaacctctt taatcataag attcaattaa gcttcttgag
    71041 ctactggata aacagacact tgttttttgt tacggccttt acgttcgaaa cgtactacgc
    71101 catcaacttt tgcaaataaa gtgtcgtctc caccgatacc cacgtttaca cctggataga
    71161 tttttgtacc gcgttgacgg taaagaattg atccacctgt aactgtttgt ccatcagcac
    71221 gtttagcacc tagacgtttt gattctgagt cacggccgtt agatgtagaa ccgccacctt
    71281 ttttgtgggc aaataattgt agattcatag ataacaacat ggtctgcacc tcctatttat
    71341 cattaattgt tttaatttgg atatattctg agttttcagc ttctatcgat tgtaatccta
    71401 ataaaagatt ttctaataga atctcagcaa tattgttttg ttcttgtgtc attcctgaag
    71461 tgacttcggt ataaagataa ccgccttctt cttcattcat ttcaacaatc ggctcaaaac
    71521 cggctaatga ggcaatcccg ttgacagtgc tgatcgctaa cgcagaaaca cccgcacaaa
    71581 cgatatcact tccataagga ccagcatcag catgcccggt caaagtgaat gaaacgatct
    71641 gacctgcgtc atttcgctta aatgttcctt tgatcatata taggccttct ttctcaaacg
    71701 ctattaagcg ttgatcgcgt cgataaccac ttttgtatat ggttgacgat gaccttgttt
    71761 acggtgtgaa tgttttttag gtttgtattt gaaagtaacg actttctttt gtttgccgtg
    71821 tttttcaaca gtaccttcaa cagttgcacc tgcaacagtt ggagcgccta cttttgtaga
    71881 ttctccgcct actaagataa cttcgtcaaa aacaactttt tctcctgctt caacgtcaag
    71941 tttttcaaca tagattgctt gtcccacttc aacttttact tgtttaccac cagttttaat
    72001 aattgcgtac atgctataaa gcacctcctt ctgatatact tagactcgcc atccgaagtg
    72061 acagcatatg ctgtgcttat tacttgttct gagcggttgt agctgtggag tccacaatta
    72121 caacagtact agcttaccag aacagcaacc gttagtcaac taaatttaaa atcaaaaagt
    72181 aaaacaaaaa ttcaagcaat acttctgctt ttctatcctc acagttctac caaataaaat
    72241 agtcgcattg tgtttctttt attcttataa taaaagaaaa aggaggagca taaatggaat
    72301 acccaaaaga actgatacaa gaagcttctt taagaatgaa cggccgtcct gtagaaggat
    72361 ttatcgctgg gcaaggacct cttcatccta aactgatgct cgtgggagaa gccccaggga
    72421 aaacagagat tgatacgcat gttcctttta gcggtcaagc tggaaaagaa ttgatgcagg
    72481 cactttcttc aactggctta acacgagaag aggtgtatat caccagtgct gtaaggagcc
    72541 gcccgtatcg cgtgactcat cggatcaata aacggacaca acagtcagaa actgtttatc
    72601 caaatcggac acctactcgt tccgaagtat ttgcgcatgc accaatattg gattacgaac
    72661 tgatgaaggt ccagccaaaa ttaattgcga cacttggaaa cattggtctt caacgtttac
    72721 ttggcaaaga atatacggtt accaaaaatc atggtcaact ctatacaggg cctgtcttac
    72781 aattaacaga acaaaaggat gcctacgaat ggtcagaaaa aaactatcga attcttccac
    72841 tttttcatcc agcagctatt ttctacaatc gctcccttgt acctgttatc catcaagact
    72901 ggcaagtact agcttcactt ttacagcaat attaaaaaga acagacaaga ccataaaatc
    72961 ttcagtttac agtcttgtct gttctttttc attgttttaa tggctaattg cagtatattt
    73021 cgcatgtaaa gctgtcaacg catgagcaac tgtttgatgt tgatgcgtga atgtatgttc
    73081 ccacaagtga gaaagttgtt tttctacttc tggctgcaag gaagtataga taatcgtttg
    73141 tcctctttgc tctgcttttt cttgaaattc atcaaaaact gcacgtgccg tcaaatcaat
    73201 acttggtacg cctcgcatgg agaaaatgac accatcgtga tgatttagtt cttccaattt
    73261 atttttgagt ttttctgaag acataaaaaa cagtggacca ctaatataca ctactgtcca
    73321 gttattcagc ttttccgttt ctggcagccc cattctttgc caatcgatcg tttctaatga
    73381 aatcgtgata gcagcgcttt tgacaacaaa aaatacacag ccgccgatca ccccgatcac
    73441 gatagcaata cttaaatcaa agataactgt acaaatcatc gtaaggataa acaatagaac
    73501 tgctgaccaa tatttattag taaatagctc cttgatcgtt tcccattcat tcatacgcca
    73561 agccgtcaca atcaaaacac ctgcaagtgc tggcattgga atctgtgaca tcactggcgc
    73621 aaaaacaagc atggagagca gtaagacaac tgcatgtatc ataccagcta atctagtttg
    73681 tgcaccagat ttgatggcca cgcttgtacg ggcgatggcc gcagtagcag gaatcccgcc
    73741 aaagaaagga agcaacagat ttccaattcc ttgcgccatt aattcttgat tgctatctaa
    73801 gggcttattt gccattcggc cagccgaagc accacatagc aagctttcaa tcattcctaa
    73861 taaagcaatg ctaatagctg gaactgccac ttgttgcaag gcagaaaagc taaaatctcc
    73921 catattcaaa cgattactac tgatcaatgt ttgtggaatc ttacccacag ttgctactgg
    73981 taaatggaac aatagcataa ctgctgttgc caaaatgata gccagcagcg aactaggcat
    74041 tcgtttcgcc catttttttg gatagataaa catccctaaa atgactagcc ctcccatgat
    74101 caaagaggct gtgtttatcg gaaatcctat cgtttgataa ctacttagtt tttcaataac
    74161 gtttgtacct tccgagtgaa caccaaataa attatcgatc tgacctaatg caataatgat
    74221 agcaatacct gaagtaaacc ccgtaattac gggtgctggg ataaaagaag tcagtgttcc
    74281 taatcggaaa agtcctgctg caagtaaaaa tagacccgct aaaaaagtag caagcaatac
    74341 accttgcatt ccctttgttg ccgcaataga catcaagata gctgccatcg cacctgtcgg
    74401 acctgatatt tgataaaaac cgcctgataa accgccaata acgataccgg caatgatagc
    74461 cgtaatcaaa cctgcggccg catcagctcc gcttgatacc ccaaaagcca aggctaaagg
    74521 caatgctaca gcagctactg tgatcccagc caaaagatct ttctgaaatc ttgaaagatc
    74581 ataacctgaa aattcttttt ttagtgagga aacataattt ttgaacacct atctaactcc
    74641 tttttcatga aattttctaa aataccattc attagaatat catatttcat gagataaagt
    74701 aatatttttc acaaagtttc ctgttagata aattcttatt ttttctatat ttataagaaa
    74761 atagaaagca atgccaacag agctagccca ccttgtttga gtaaaatcca acgatcgcta
    74821 gtcaaactgc catataatgc aacaaaaaaa atagatatta atagcatact tgtgatttct
    74881 ttagatgcgg aagaaagata agctccataa agtagtccca ctcctaatag tccattgtac
    74941 actccttggt tcttaaacaa tgtttgaaca gattttctgc ttaactcttc tttactcatt
    75001 ttaaaaaccc tacttgtcgt gtcagaatca gttctcaccg tttcgatata catgatataa
    75061 aaaaattcaa ttgcaactag agtcactaaa attctagata gtatcgtcat atcttcttcc
    75121 tcttttctga tgagttttat ttttttgctc cttataattg tactcgataa ggatgttttt
    75181 ttattttatt tgcctttgaa aacaaacaat attggcacaa aaaacaaaga aaaaccgtta
    75241 gaaacctcag tttctaacgg ttttttagcg tcacaggagg gattcgaacc cccgaccgtc
    75301 cgcttagaag gcggatgctc tatccagctg agctactatg acaagaatca gcggaacatc
    75361 aataattata gataattaaa ttgcttctgt caatacgaaa tagttattta attagcaaat
    75421 aatgaattat tttggcccaa ctactaaatc aatcttttaa aacgagacat tctccttcag
    75481 ctgttctaag gttatcaatt gaaaacagga gctacattga cttcatgcta ctcctgttta
    75541 tttagatttc ctgtatcctt taattgactt acaaatatag aaacttttac atcaagttcg
    75601 aattgagccc tcgattcaat cgattatcta caggctgcca taatcatgta tcacaactcg
    75661 attgtttctg attcctttgt cattaagatc acaccatcac ctaatggcaa gattgtcgat
    75721 gtcaaatcgg gatgatccat cacaacacga agaaattcat ttaatttacg atggatagct
    75781 cgtttgccac ggggaatctc ttcatctggg agcaagatcg ttcctccttg gaagatgtca
    75841 tctaccatca aaacgccacc ttttcttaac aaacgtaagc attcaggtaa aaattcaata
    75901 tattttgatt ttgcactatc cataaaaata aagtcatacg gaccagacaa ctccggcaag
    75961 atatctgctg cttgaccttc aagcaaagtc actttatctg tcagtcctag acgttcatag
    76021 ttttttttag ctttctcgat catgacatcg aaacgatcaa ttgtcgtaac atgagcgttt
    76081 tctccgattg tcgtggccat gacacttgat gaaaaaccga tagctgctcc gatctccaag
    76141 attcgttcag gctgaagctg tccaagtaaa aatttcaaaa atacaactgt ttcatgggga
    76201 ataactggta cttctgctac atgagcttct tcttccagtt tgcctagctc acccggtaat
    76261 tttttttgct gggtacgcat aaaatccaat agttcttctt tgactacagg acgatgcatc
    76321 atttcattgc gcatactact cctcctcttc acacattata cgaacaactg actaaatcta
    76381 caagctgcat gagtgttttc tcgtgtttca tgttcagaag agtcgttgga tctctggaat
    76441 acatttatgt ttttaacatt cttacaaaaa tttctgtgtt cgtctccgct tctttttcaa
    76501 ctctacaaag gtattccatg ctgtgattcc tgttaggcaa aaagcaatcg taaagaataa
    76561 cccctgaatc catgcaaatg ccgtttttga aaggactagt ccaatatatg gaatctgaaa
    76621 gatgggacta ccgataatat ttctcttttt tactactcca ggctcaatat aagtctttgt
    76681 atcagctttt gtataaaaat cttcgtggtt gttttcaacg gctattacgc gacgcgtcga
    76741 gatttttttt cctgaatttt cataatatgt aataatatct ccgactaaaa tctcatttgt
    76801 atttttcggt cgtacatata tcagatttcc ttgtgcataa tatggactca tttctttgtc
    76861 ctcaataata taaggagtca aaccaaaaat tcggggaacc aagacgacga gaagaataag
    76921 aattaaaaat ataatcaacg ttatagaact tataaaagtt atttttttgt aatcaggtat
    76981 cgttttcaac ttctttcttt attcaatcct tcttaatcat ataataacga taaatttttt
    77041 caaattatag ctgtaaaaat gtttcccttt aataattata tgtttctaat tctatttcct
    77101 c
//
LOCUS       DACDHL010000003        74035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDHL010000003 DACDHL010000000
VERSION     DACDHL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.003"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..144)
                     /locus_tag="IV228_000204"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="IV228_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="IV228_000205"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="IV228_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8716156.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1716..2498)
                     /locus_tag="IV228_000206"
     CDS             complement(1716..2498)
                     /locus_tag="IV228_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716157.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2563..3399)
                     /locus_tag="IV228_000207"
     CDS             complement(2563..3399)
                     /locus_tag="IV228_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8716158.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3417..4826)
                     /locus_tag="IV228_000208"
     CDS             complement(3417..4826)
                     /locus_tag="IV228_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8716159.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4840..6663)
                     /locus_tag="IV228_000209"
     CDS             complement(4840..6663)
                     /locus_tag="IV228_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8716160.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7506..7832)
                     /locus_tag="IV228_000210"
     CDS             complement(7506..7832)
                     /locus_tag="IV228_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716161.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7878..8240)
                     /locus_tag="IV228_000211"
     CDS             complement(7878..8240)
                     /locus_tag="IV228_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HAP8716162.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8277..8491)
                     /locus_tag="IV228_000212"
                     /pseudo
     CDS             complement(8277..8491)
                     /locus_tag="IV228_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8619..8954
                     /locus_tag="IV228_000213"
     CDS             8619..8954
                     /locus_tag="IV228_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8716163.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9030..11027)
                     /locus_tag="IV228_000214"
     CDS             complement(9030..11027)
                     /locus_tag="IV228_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716164.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(11125..11400)
                     /locus_tag="IV228_000215"
     CDS             complement(11125..11400)
                     /locus_tag="IV228_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8716165.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11632..12366
                     /locus_tag="IV228_000216"
     CDS             11632..12366
                     /locus_tag="IV228_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8716166.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12405..13547
                     /locus_tag="IV228_000217"
     CDS             12405..13547
                     /locus_tag="IV228_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8716167.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13642..15033)
                     /gene="sufB"
                     /locus_tag="IV228_000218"
     CDS             complement(13642..15033)
                     /gene="sufB"
                     /locus_tag="IV228_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8716168.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15038..15508)
                     /locus_tag="IV228_000219"
     CDS             complement(15038..15508)
                     /locus_tag="IV228_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8716169.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(15495..16730)
                     /locus_tag="IV228_000220"
     CDS             complement(15495..16730)
                     /locus_tag="IV228_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8716170.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16727..18013)
                     /gene="sufD"
                     /locus_tag="IV228_000221"
     CDS             complement(16727..18013)
                     /gene="sufD"
                     /locus_tag="IV228_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8716171.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18026..18799)
                     /gene="sufC"
                     /locus_tag="IV228_000222"
     CDS             complement(18026..18799)
                     /gene="sufC"
                     /locus_tag="IV228_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8716172.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18996..19844)
                     /locus_tag="IV228_000223"
     CDS             complement(18996..19844)
                     /locus_tag="IV228_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8716173.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(19868..20554)
                     /locus_tag="IV228_000224"
     CDS             complement(19868..20554)
                     /locus_tag="IV228_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716174.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(20547..21581)
                     /locus_tag="IV228_000225"
     CDS             complement(20547..21581)
                     /locus_tag="IV228_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8716175.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(21976..22314)
                     /locus_tag="IV228_000226"
     CDS             complement(21976..22314)
                     /locus_tag="IV228_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8716176.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22316..22672)
                     /locus_tag="IV228_000227"
     CDS             complement(22316..22672)
                     /locus_tag="IV228_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8716177.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(22678..23865)
                     /locus_tag="IV228_000228"
     CDS             complement(22678..23865)
                     /locus_tag="IV228_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8716178.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24056..24901)
                     /locus_tag="IV228_000229"
     CDS             complement(24056..24901)
                     /locus_tag="IV228_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8716179.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25135..26100
                     /locus_tag="IV228_000230"
     CDS             25135..26100
                     /locus_tag="IV228_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8716180.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            26477..27520
                     /locus_tag="IV228_000231"
     CDS             26477..27520
                     /locus_tag="IV228_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8716181.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(27582..28046)
                     /locus_tag="IV228_000232"
     CDS             complement(27582..28046)
                     /locus_tag="IV228_000232"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8716182.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            28292..29239
                     /locus_tag="IV228_000233"
     CDS             28292..29239
                     /locus_tag="IV228_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8716183.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(29450..31522)
                     /locus_tag="IV228_000234"
     CDS             complement(29450..31522)
                     /locus_tag="IV228_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8716184.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(31455..31832)
                     /locus_tag="IV228_000235"
     CDS             complement(31455..31832)
                     /locus_tag="IV228_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8716185.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(31961..32521)
                     /locus_tag="IV228_000236"
     CDS             complement(31961..32521)
                     /locus_tag="IV228_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8716186.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            32671..33963
                     /locus_tag="IV228_000237"
     CDS             32671..33963
                     /locus_tag="IV228_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8716187.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(34023..35141)
                     /locus_tag="IV228_000238"
     CDS             complement(34023..35141)
                     /locus_tag="IV228_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8716188.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(35155..36576)
                     /gene="arcD"
                     /locus_tag="IV228_000239"
     CDS             complement(35155..36576)
                     /gene="arcD"
                     /locus_tag="IV228_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8716189.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(36822..37943)
                     /gene="mnmA"
                     /locus_tag="IV228_000240"
     CDS             complement(36822..37943)
                     /gene="mnmA"
                     /locus_tag="IV228_000240"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8716190.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            38347..38946
                     /locus_tag="IV228_000241"
     CDS             38347..38946
                     /locus_tag="IV228_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8716191.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(39084..40370)
                     /gene="efmA"
                     /locus_tag="IV228_000242"
     CDS             complement(39084..40370)
                     /gene="efmA"
                     /locus_tag="IV228_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8716192.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(40641..40985)
                     /locus_tag="IV228_000243"
     CDS             complement(40641..40985)
                     /locus_tag="IV228_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8716193.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(41122..42258)
                     /locus_tag="IV228_000244"
     CDS             complement(41122..42258)
                     /locus_tag="IV228_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8716194.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(42447..43421)
                     /locus_tag="IV228_000245"
     CDS             complement(42447..43421)
                     /locus_tag="IV228_000245"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8716195.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(43501..44376)
                     /locus_tag="IV228_000246"
     CDS             complement(43501..44376)
                     /locus_tag="IV228_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8716196.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            44506..46413
                     /locus_tag="IV228_000247"
     CDS             44506..46413
                     /locus_tag="IV228_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8716197.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(46954..50232)
                     /locus_tag="IV228_000248"
     CDS             complement(46954..50232)
                     /locus_tag="IV228_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8716198.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(50503..51048)
                     /locus_tag="IV228_000249"
     CDS             complement(50503..51048)
                     /locus_tag="IV228_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8716199.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(51060..51752)
                     /locus_tag="IV228_000250"
     CDS             complement(51060..51752)
                     /locus_tag="IV228_000250"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8716200.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(51771..52055)
                     /locus_tag="IV228_000251"
     CDS             complement(51771..52055)
                     /locus_tag="IV228_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716201.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(52068..52631)
                     /locus_tag="IV228_000252"
     CDS             complement(52068..52631)
                     /locus_tag="IV228_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8716202.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            52859..53521
                     /locus_tag="IV228_000253"
     CDS             52859..53521
                     /locus_tag="IV228_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8716203.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            53645..54841
                     /locus_tag="IV228_000254"
     CDS             53645..54841
                     /locus_tag="IV228_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8716204.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(54942..56318)
                     /locus_tag="IV228_000255"
     CDS             complement(54942..56318)
                     /locus_tag="IV228_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8716205.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(56355..57221)
                     /locus_tag="IV228_000256"
     CDS             complement(56355..57221)
                     /locus_tag="IV228_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8716206.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(57310..58335)
                     /locus_tag="IV228_000257"
     CDS             complement(57310..58335)
                     /locus_tag="IV228_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8716207.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(58353..59027)
                     /locus_tag="IV228_000258"
     CDS             complement(58353..59027)
                     /locus_tag="IV228_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8716208.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(59211..60104)
                     /locus_tag="IV228_000259"
     CDS             complement(59211..60104)
                     /locus_tag="IV228_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8716209.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(60178..61875)
                     /locus_tag="IV228_000260"
     CDS             complement(60178..61875)
                     /locus_tag="IV228_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8716210.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(61868..63181)
                     /locus_tag="IV228_000261"
     CDS             complement(61868..63181)
                     /locus_tag="IV228_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8716211.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(63181..67218)
                     /locus_tag="IV228_000262"
     CDS             complement(63181..67218)
                     /locus_tag="IV228_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8716212.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(67231..67689)
                     /locus_tag="IV228_000263"
     CDS             complement(67231..67689)
                     /locus_tag="IV228_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8716213.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(67987..68505)
                     /locus_tag="IV228_000264"
     CDS             complement(67987..68505)
                     /locus_tag="IV228_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8716214.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(68507..69124)
                     /locus_tag="IV228_000265"
     CDS             complement(68507..69124)
                     /locus_tag="IV228_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8716215.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(69121..69975)
                     /locus_tag="IV228_000266"
     CDS             complement(69121..69975)
                     /locus_tag="IV228_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8716216.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(69972..70883)
                     /locus_tag="IV228_000267"
     CDS             complement(69972..70883)
                     /locus_tag="IV228_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8716217.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(70924..72258)
                     /locus_tag="IV228_000268"
     CDS             complement(70924..72258)
                     /locus_tag="IV228_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8716218.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            72466..73296
                     /locus_tag="IV228_000269"
     CDS             72466..73296
                     /locus_tag="IV228_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8716219.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(73540..73789)
                     /locus_tag="IV228_000270"
                     /pseudo
     CDS             complement(73540..73789)
                     /locus_tag="IV228_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        21383 a        15696 c        13003 g        23953 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
     1441 tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt
     1501 tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa
     1561 ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa
     1621 tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg
     1681 ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact
     1741 tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt tttcccattt
     1801 tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct
     1861 gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta
     1921 tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat
     1981 gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca
     2041 ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca
     2101 ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc
     2161 atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag
     2221 acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg
     2281 ttgtttacat ctacataata atcctttgac tctacttgag accttcctat acggaataca
     2341 gctttactgt ctgatatctc tataccagca tccttattat taatctgcca atctccaagt
     2401 agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc
     2461 gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa
     2521 ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat
     2581 atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac
     2641 gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc
     2701 cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa
     2761 ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg
     2821 aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc
     2881 ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc
     2941 ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat
     3001 ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat
     3061 gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact
     3121 tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat
     3181 aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga
     3241 aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt
     3301 tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca
     3361 aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa
     3421 gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt ttttattcgt
     3481 tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg
     3541 aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct
     3601 acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga
     3661 tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca
     3721 gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca
     3781 gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg
     3841 tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat
     3901 ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg
     3961 ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga
     4021 tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa
     4081 gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta
     4141 gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga
     4201 tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt
     4261 tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc
     4321 atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc
     4381 aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc
     4441 tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga
     4501 ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta
     4561 ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat
     4621 tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa
     4681 ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc
     4741 tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga
     4801 aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg
     4861 ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt
     4921 cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt
     4981 gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc
     5041 cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga
     5101 acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga
     5161 ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa
     5221 gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat
     5281 gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag
     5341 ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat
     5401 attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc
     5461 atatctgact aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga
     5521 agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc
     5581 cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt
     5641 cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc
     5701 attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca
     5761 agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca
     5821 agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc
     5881 aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt
     5941 agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat
     6001 gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg
     6061 aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag
     6121 caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc
     6181 ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat
     6241 gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt
     6301 taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact
     6361 aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt
     6421 tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg
     6481 gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt
     6541 atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct
     6601 gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc
     6661 cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca
     6721 ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat
     6781 tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt
     6841 tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata
     6901 tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat
     6961 tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg
     7021 atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga
     7081 tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa
     7141 aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg atagaatgat
     7201 ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta
     7261 catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat
     7321 aattttttct ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg
     7381 acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa
     7441 aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat
     7501 ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc
     7561 tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc
     7621 cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag
     7681 ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc taatccagat
     7741 gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat aactataatg
     7801 ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt
     7861 tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca
     7921 gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg atataagtag
     7981 cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc tactaccata
     8041 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
     8101 ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc agttgtctca
     8161 tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt tatttctttg
     8221 ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt tattagttaa
     8281 ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact taatatctca
     8341 gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
     8401 cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct ttttcttttc
     8461 tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt attatataca
     8521 acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca ttacaaatac
     8581 aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa
     8641 aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa
     8701 tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg
     8761 atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt ctagacaatg
     8821 ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg
     8881 cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc
     8941 tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga
     9001 aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc
     9061 cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg aactaattca
     9121 actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt tgctaggtag
     9181 actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt agaagctcgt
     9241 tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc attttccacg
     9301 ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc tttctctgtc
     9361 attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc
     9421 aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct tttagcctct
     9481 ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaaat
     9541 ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa
     9601 acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc
     9661 tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt
     9721 atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt
     9781 ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca
     9841 ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc
     9901 atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg
     9961 tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg
    10021 ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca
    10081 actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc
    10141 ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt
    10201 aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc
    10261 tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct
    10321 tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag ttcatactct
    10381 ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca
    10441 gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca
    10501 aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag
    10561 tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca
    10621 atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa
    10681 ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact atcatcttta
    10741 ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt
    10801 actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata
    10861 aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta
    10921 tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt ctctaataat
    10981 ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc
    11041 ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta
    11101 tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc
    11161 agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac
    11221 atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat
    11281 atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat
    11341 ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat
    11401 atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt
    11461 tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt
    11521 tcatcgacat acacataata cctcgataaa aaagaggtgt caatatcttt gtaaaattta
    11581 tttacaacac ccctttttcg aggtatacta attttaggag gtgtggtcaa tatgataaaa
    11641 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
    11701 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
    11761 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
    11821 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
    11881 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
    11941 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
    12001 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
    12061 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
    12121 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
    12181 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
    12241 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
    12301 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
    12361 atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca aaaatatcaa
    12421 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
    12481 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
    12541 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
    12601 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
    12661 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
    12721 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
    12781 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
    12841 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
    12901 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
    12961 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
    13021 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
    13081 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
    13141 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
    13201 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
    13261 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
    13321 ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac
    13381 atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat
    13441 tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac
    13501 ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa
    13561 aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta tatatcaagg
    13621 gttctagctc ctataacagg tttacccaac actgccttcc atctcataac tgatcaatcg
    13681 attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa caaagcccat
    13741 gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat agtagagttg
    13801 ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc tgttgtggat
    13861 ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg tgtcacattc
    13921 gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc gatagtttac
    13981 ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat ttggtgcatt
    14041 gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg cgatggagag
    14101 catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca tcgttgtttt
    14161 tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt
    14221 ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac ggcaatacgc
    14281 atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg aataagtagg
    14341 tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga tcagcgtacg
    14401 ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg gaacatcgac
    14461 tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg aattcagcgc
    14521 tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga ataattcagg
    14581 gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt cgaattcatc
    14641 tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg ctagataagc
    14701 tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt ctggtacgtc
    14761 ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg catcgaaatc
    14821 gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg tttctagaga
    14881 tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca tctcacggat
    14941 cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc
    15001 gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc ttgtggtaat
    15061 gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc caggcaagtg
    15121 tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca gcatcgccta
    15181 attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct acagttaaat
    15241 gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag gcacttgccg
    15301 tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg tcttttacat
    15361 cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc ttgcttgtcg
    15421 agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc acttgacggt
    15481 ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc catgattgct
    15541 tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc
    15601 gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc tctgacggct
    15661 accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc caaattgaat
    15721 gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa tccatcaatt
    15781 gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg gatattgtcc
    15841 ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc ggcgatattt
    15901 ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta taattcgaca
    15961 aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca ttttcgttta
    16021 ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa agcaaagaaa
    16081 tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc accatctact
    16141 acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg attgaagaca
    16201 cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt aatctttttc
    16261 gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt caagacagca
    16321 cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc catgtaagag
    16381 agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc tacccaattc
    16441 aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc atggatgaac
    16501 gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg
    16561 acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc ctggagtact
    16621 tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc atcattgacg
    16681 tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat ccattcaact
    16741 tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt gctgttaaga
    16801 cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc aatcctctac
    16861 tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca tgtccagcag
    16921 ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct ttatcagaaa
    16981 gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc aagatgtggc
    17041 cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc aaaatatgcc
    17101 cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt tgtctgcccg
    17161 agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa
    17221 agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca gaagcatctc
    17281 gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat tgatctacgg
    17341 ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata ttacctgatg
    17401 attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat tcactatgtt
    17461 catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga
    17521 ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg aaaataccgc
    17581 tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt actgcttttt
    17641 tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac aagtcggtaa
    17701 aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc catgaacctt
    17761 gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg cctgtttcac
    17821 tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc acccgatcga
    17881 tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc
    17941 aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa tcgagccagt
    18001 ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg attcctaatt
    18061 ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca gcattccctg
    18121 tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata tagttcaata
    18181 atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt tcatttactc
    18241 ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg tctaaaattg
    18301 caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt tctccgccag
    18361 aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt aaagccattt
    18421 tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca tcacgtttcg
    18481 cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca cttggatatt
    18541 gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct aaaacatttt
    18601 tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc atgatagcgg
    18661 cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt tctccggttt
    18721 tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca tgtagatttt
    18781 ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc actcatttta
    18841 gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc ttttatttat
    18901 tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga aataagccat
    18961 tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc ctttactggt
    19021 ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg gtgaagcact
    19081 tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc aataatattc
    19141 acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt caaccctgcg
    19201 ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg gtacgtagaa
    19261 gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt ttcttcaata
    19321 tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa gccagcatct
    19381 tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat gattgttgca
    19441 ccatctttta attcggaaac atctttgact tttttagagt acaagcccat tggttcgatg
    19501 tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt gttcaaataa
    19561 ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg gttcggtaat
    19621 acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa taatggtttc
    19681 acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg
    19741 tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc taatcctaca
    19801 gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta catcccccta
    19861 tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct tgaacgataa
    19921 atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta ccacgctggt
    19981 agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag
    20041 taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg ggaatacttt
    20101 caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca tctgctgctt
    20161 ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca taaaatggtg
    20221 ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg cctacgattg
    20281 ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac cggaaaatat
    20341 tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt ttgcgtccaa
    20401 tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc attgagatta
    20461 atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg atttgtttaa
    20521 aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat tcctactcct
    20581 tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt catcaagaca
    20641 gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac aacgttgaca
    20701 tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt tctttcgcta
    20761 aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc taatagttct
    20821 gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac ttcttttttc
    20881 ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa ctccatcaca
    20941 gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc
    21001 aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt tgtttgcggg
    21061 tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc caatgctcgt
    21121 gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc ttgtccgcgt
    21181 ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt gctctttggt
    21241 acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga ccataacagg
    21301 ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc tttgtttttt
    21361 aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca
    21421 ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat tccataaata
    21481 tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc gtttttccca
    21541 ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac atctttctct
    21601 tataattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt
    21661 gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct cttccagtac
    21721 ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg tagtaagttt
    21781 gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt tacccatttt
    21841 cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca aagctttctc
    21901 gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca acttttttca
    21961 agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa
    22021 gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac cgattttatc
    22081 attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg ctttttccgc
    22141 ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg gcagattggc
    22201 aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac agtattcttt
    22261 tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg ccattttata
    22321 tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg tcctctcgta
    22381 agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc gcttttgaag
    22441 ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc ccgcttgtgt
    22501 tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc gttgaggcct
    22561 tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg tctaaccacg
    22621 ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac aattttctta
    22681 cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata tcaacccgat
    22741 accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca atgggatccc
    22801 tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga acagcagcat
    22861 catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga aacacacccg
    22921 aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac cgataaagcc
    22981 gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca catagatatt
    23041 gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa gtccttgggc
    23101 cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat caatacgtgc
    23161 aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt cagtcgtaac
    23221 caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg cgatgatgcg
    23281 ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga caagcatcgt
    23341 tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga tcaagagctt
    23401 cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat tatgtttagt
    23461 tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg gctggaaagt
    23521 aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat tctgatcata
    23581 gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag gagtcagttt
    23641 ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc caactaaata
    23701 ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat catggctaag
    23761 tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta aaatcacgcc
    23821 ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc atcccttttc
    23881 ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa gtctttccat
    23941 agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa
    24001 agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca attatctacc
    24061 agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga aggatctctt
    24121 cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa taaaaatatc
    24181 gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta gacaaaatcg
    24241 agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc acgatcatct
    24301 tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt
    24361 ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact agctggtctt
    24421 ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata aaaccaaaat
    24481 cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt tcgatctcaa
    24541 aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa agatagacag
    24601 caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca ttgagcaatt
    24661 tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat aataagtcgg
    24721 cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg atattcaatt
    24781 cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac agtacttctg
    24841 ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat aacttaaaca
    24901 tggcaccact tccataactt tttatgataa ttactataaa aactatttga tttaaatata
    24961 caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa ctagtaactt
    25021 agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc attttacaat
    25081 gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt atatatgaag
    25141 aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt agcttccctt
    25201 ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg cggtatctct
    25261 atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa
    25321 acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc actcatggtt
    25381 atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt catttggctg
    25441 tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca tgttatcaaa
    25501 gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt tggatttggg
    25561 gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt cttttggatt
    25621 tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt tttcgtaaga
    25681 aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg ttctctttgc
    25741 ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt agcattgttc
    25801 gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt
    25861 cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc aacagctata
    25921 tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt ggatttctta
    25981 aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc tcattacgta
    26041 cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa
    26101 taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga acggccaaac
    26161 aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg caagcttttg
    26221 aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata agaaggaatt
    26281 tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag aagctacttc
    26341 tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga cctcttgttc
    26401 ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt atttattgac
    26461 gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa aggattcagc
    26521 ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct tcagcaggct
    26581 tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat tgggtatttt
    26641 attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat ttgctgaaag
    26701 caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc aaacgattgc
    26761 tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca ctatcatcgg
    26821 tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga tgatggctgg
    26881 aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca tccatgcgaa
    26941 agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg tgatgatgtt
    27001 gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa aaagtgcact
    27061 tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct tacttgacaa
    27121 ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc tcgttgttgt
    27181 tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg aaacgccaac
    27241 tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt ttgtgatctt
    27301 ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg cacattttat
    27361 cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag actttgcaaa
    27421 attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc ttctccaaac
    27481 agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg aaaaaacagt
    27541 tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg cagatatcct
    27601 tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc catatatcct
    27661 gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg caatggattg
    27721 gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc aacggctact
    27781 tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca gacattttgc
    27841 tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct ttttcccgca
    27901 aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc tgattcgctt
    27961 tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc taaccataaa
    28021 aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac tggaacttcc
    28081 tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta aaagtttgat
    28141 aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac ctgttctttt
    28201 acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa tgtaaaccat
    28261 atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg tcccatgttt
    28321 taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca aaggtgccca
    28381 tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag cagcaatcaa
    28441 gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa attttgggaa
    28501 agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga ccgtgatgga
    28561 tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga tcactacttc
    28621 tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg cgatacgcag
    28681 tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag aaatggtcga
    28741 tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc tagaattaac
    28801 agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa cagaatatat
    28861 ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct ggaaattatt
    28921 caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga ttgcttcatt
    28981 tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta ttttgcgggc
    29041 attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct tcctgttcat
    29101 tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga aaatcttctt
    29161 agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga agccaaatga
    29221 attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa atgaaaaagg
    29281 actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc cagaagtagc
    29341 ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt
    29401 cttatttctt gaagctgacc acttctatca caacctcttt catatcttat tacttcttca
    29461 ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct tgtgcatatt
    29521 ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct tttttatcaa
    29581 gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt ttaccttcga
    29641 tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt ggatcttttc
    29701 ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca cgtgtgtttt
    29761 tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag aaatcttgat
    29821 attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt tcgattccat
    29881 tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct ttttcccagc
    29941 tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat tgcttacctt
    30001 taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg attagtttct
    30061 cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt tgaccaagca
    30121 agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg gcgaaatcgg
    30181 gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca gtttcgcctt
    30241 tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta ccaaattcaa
    30301 ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa aaacgagaga
    30361 caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt gctctgtttt
    30421 cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact tttgtctgtt
    30481 ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg ctgactgctt
    30541 gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg tctgtacgcg
    30601 gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat gttttctttg
    30661 cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca taaggcaatg
    30721 gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg cttgtactga
    30781 tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga tttttttgaa
    30841 gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt tgtggacgga
    30901 actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc tgcacacggc
    30961 ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc aatcctacca
    31021 gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat tctttcgcag
    31081 gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag atccataacc
    31141 gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca agttcccctt
    31201 ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga gccagctgct
    31261 tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt tttttaggga
    31321 tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct tccggcattt
    31381 tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct tcataataat
    31441 ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg cttggttttt
    31501 ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat gggctgatga
    31561 tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc ttcacaccag
    31621 cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt gatatattca
    31681 taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc ttcgaaagac
    31741 tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca ccaaggctca
    31801 ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc agcttttcta
    31861 gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa aagggtcatt
    31921 taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa gtagcgataa
    31981 agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct tttgtaaagt
    32041 atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc ctgcttcgat
    32101 gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg atctgattcg
    32161 tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg atcagtaact
    32221 catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag atctgcagtg
    32281 cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt tcctgcagaa
    32341 gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta tacagcgagg
    32401 gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga acatccatct
    32461 cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt ctttttttca
    32521 tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca cgaaaaagaa
    32581 agagaaaaaa gagactttca ttgataaact acatattgta gtttataata aagagctgta
    32641 gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga aaaattattt
    32701 aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac atttatggct
    32761 ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac gttaggtaat
    32821 tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac tttcttggtg
    32881 actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag aaaattgatg
    32941 ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg cgtgactagt
    33001 gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta tatcagtaat
    33061 gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt tcttggaacc
    33121 ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg gatctctgct
    33181 tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt tttcgtcttc
    33241 ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga aaacatggtt
    33301 tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg tatgttcgtc
    33361 acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag tctgtacatt
    33421 cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat tgcttcgata
    33481 cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga caagattggg
    33541 agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat cccaatggca
    33601 ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat ctctgatgct
    33661 tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca agctgctgga
    33721 cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg gcctatgatc
    33781 ggctccagtg tttccagtgc atttggttat cgcggcgtct ttctttcgac ttctcttctt
    33841 gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca gcaagaaact
    33901 gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc tcatagacaa
    33961 taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg cagtgttttt
    34021 gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc caaggtttct
    34081 tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact tcctggtact
    34141 aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc ttcttcgatg
    34201 gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac tttcggttca
    34261 ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt gtttcgcgcc
    34321 aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag gactccgccg
    34381 caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg tccagtagct
    34441 gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt ggttgctata
    34501 cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa aggcgcatat
    34561 acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat tttttctaaa
    34621 gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca gatgattttt
    34681 gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc ttcttcttcc
    34741 ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata taattgctga
    34801 taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta tagagctaaa
    34861 agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac ggtttgataa
    34921 agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata gtccaatggt
    34981 tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc aacgagttcg
    35041 tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt cttttcccat
    35101 ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc ccccttatat
    35161 cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac aaatcagccc
    35221 aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag cgatccagta
    35281 gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa atcctgctaa
    35341 aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt gttttttgat
    35401 tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac aaatcaaaat
    35461 tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta agaaagtcaa
    35521 taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg catttttttt
    35581 attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca tcgttgtttc
    35641 tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca agccgatatt
    35701 gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca tagcaggttg
    35761 tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg aagcaaggat
    35821 atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt ctgaacgttt
    35881 cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc acatcataac
    35941 catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc cattcactag
    36001 attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa atgcgataaa
    36061 tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga ttgtattgat
    36121 gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa taaaaatact
    36181 tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa attctcccat
    36241 ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca accagtaacc
    36301 ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac cgaaaatacc
    36361 accatctaga tctggacgct tttctcctaa atgattcagc gataatacca aagctaaaat
    36421 accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag aactagccag
    36481 atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca gagcaaccag
    36541 tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca cttataaaat
    36601 tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc ttaacaaaaa
    36661 gcaactttat tcatattatt ggatattatg aactttttac acaagtattt caagtataaa
    36721 atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag aaaaggtgtt
    36781 tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta ttgtaaaact
    36841 tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat tccatcataa
    36901 aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac gacttctgca
    36961 cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata acggaatttc
    37021 gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat ttcacttgca
    37081 tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata taatgtgttt
    37141 gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc gccaccgatc
    37201 cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg tcctttgact
    37261 tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct caaaaattgt
    37321 ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt agcagtcgca
    37381 aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc tagagggaac
    37441 atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg atctttatta
    37501 ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac ttgcgcataa
    37561 tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa tgctttgaat
    37621 ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg gtattcggct
    37681 aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga gtaataagga
    37741 atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc cgtgcataca
    37801 ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc gtatccttgt
    37861 tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc tacgacaacg
    37921 cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc tgaaacatct
    37981 acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt ctgaaaattt
    38041 catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca gtacacaaac
    38101 atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa attggcatac
    38161 tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt gaaaaactat
    38221 ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc ttttcttttt
    38281 agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa gggaggcaaa
    38341 agatacatga attactttat cagtgatatg cactttttcc atgaacgctt attaggaaaa
    38401 aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca gttgatcact
    38461 agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga ccttgctatg
    38521 catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca actgcaagga
    38581 acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta tttagaaaaa
    38641 aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt aggtgccatc
    38701 gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct cgggcctaac
    38761 gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat cgagaacgat
    38821 gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact accatttggc
    38881 actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa gtacgatagg
    38941 tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac tcatattttt
    39001 catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt gtaacaagac
    39061 ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc ttttaccagt
    39121 tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa aacgattccg
    39181 cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa acgatcagct
    39241 aatggtcctg caaataataa tccaattgga cctgccaggt tcatcaaaga gttgaagttc
    39301 cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc catcagcagt
    39361 gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa gtacaagaac
    39421 ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat aatgatgtag
    39481 gctataataa taggtttcat tcggtctttc catgttccta taaaactgat cagtgcaccg
    39541 cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc aacggaacca
    39601 ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa caacagcatc
    39661 acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa ttgctgcagc
    39721 ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg agaaactttt
    39781 gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac atctaataaa
    39841 ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg cgcaatgatg
    39901 aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg agaagatgga
    39961 acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc gaccgaacgc
    40021 ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt tccgactact
    40081 gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag agctttttta
    40141 tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg aatcattcct
    40201 agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa ataccagatg
    40261 atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga taaaaagaac
    40321 aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat aaacatccct
    40381 cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga ttcttttttc
    40441 aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt acttagaaac
    40501 aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat aaaaaagaca
    40561 actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt caaaactagt
    40621 tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct aatcatccca
    40681 tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt gaaaatattg
    40741 actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat atcttttgat
    40801 acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa tggtcggatc
    40861 aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt atatcttttt
    40921 gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt tgtttcaaat
    40981 gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt atcctgattt
    41041 ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag acatctgttc
    41101 atataagaat gacttcacta gttatttttt gacagcacga actaattgat caaggaaata
    41161 ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt cttttagtat
    41221 cggtgtatct tctccataca ttgcttctaa tacgtgtgaa ggctcgatat ttcctgctgt
    41281 acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca gtagcaaatc
    41341 atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg ttaaatcacc
    41401 atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt gctggaatcc
    41461 tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa ctgcttttgc
    41521 cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt cctgctcccc
    41581 gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac cgacgccttt
    41641 tggcccattg attttatgag cagagatact taataaatcg attcctaatt cttctggatg
    41701 gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg gatgatccgc
    41761 taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt tcccgtacat
    41821 cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga tcgacagatt
    41881 ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta aatatttcat
    41941 ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc cttctttttg
    42001 acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc cactgttaaa
    42061 gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg ctgtttccaa
    42121 tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa acgtgtcttg
    42181 catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg ccgcatgatc
    42241 cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca cattctagca
    42301 taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt tcatgggcat
    42361 agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct cacttctgtc
    42421 acaacttctt tccctctttt ttttgtttat tcgaattctt tttcttccag acggaataac
    42481 ggactcatcg gtgtattttc atggatgcgt ttgaccgctt cacccattaa ttctgaacat
    42541 gtaacgattg ttaagttttc tggatgacga ttttcttctg tgaaaacaga atcagtgata
    42601 caaatatctt tgattggtgc attgtctaaa atacttttcg catgatcgga caataatcca
    42661 tgtgacgcac aggcatatac ttcttttgcg ccgttttctt ttaatacatt tgctgctcgt
    42721 gcaagcgttg ctcctgtgtt caagatatca tctaccatga tacatgtctt tccttgaaca
    42781 tcaccgatca cataaccacc ctcagttgaa gcatcatctg catgatctac gatggctaat
    42841 gtgctgttca aatattctga aagactacgt gcacgctgaa caccgctgtt ttttggcgaa
    42901 acaactacga tgtcatctcc cgtcagacct aattgacggt aataatgagc aaacaacggc
    42961 atcgtaaata aattatctac aggaatatca aagaatcctt gtacttgaac cgtgtgcaga
    43021 tctaaagtca gtactcgagt tgcccctgct tcttcgatca agttagcgat caatttcgca
    43081 gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag catacccata gtaaggcaat
    43141 acgacattga tcgttttcgc acttgctcgc ttcattgcat cgatcatgat cagtagttcc
    43201 atatagtaat cattgacagg tgcattcgtt gattgaacga tatacacatg atcccctcga
    43261 atactttctt cgatattgat ggaaatctcg ccatcactaa attgcttcac gacgctttta
    43321 cctaattctg ttccgaacac tttcgcgatt ttttcagcta atggacggtt gccattcaaa
    43381 ctaaaaatct tcaacgtgtc atcctgatac ttattactca tgtagtcctc cccaaaactt
    43441 ctctattctt cgatagttta acatattttt ccaagatttt ctgttaattt cagcaaattc
    43501 ttaaggaagt gaaactgttc cgttactcca actggcagga atgactttat gataatcgcc
    43561 ccagttgtcg tgtactttgt aataacgttc tcctgtttca gtttcaaaat aagcgtacgc
    43621 tgtcacccaa tgattgccat atgtactgcc aagtactttc aaaataccaa tcatcacagg
    43681 tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa cctagaagag ttgctcgtgc
    43741 acgctcatga ttgccattct tctttaaaaa actagaaatc cctgtactaa cctgaaaagg
    43801 aatcgtagga agtcctagtg gttggatctg gctacgcaat gccttcgtca aaaccaacga
    43861 atcagccgta tttttcttgc gaatctcatt tggaagcatc caccccttac gatagtcttg
    43921 ataataggca aggagaactg ctgccgcgta agtaccacaa agatagcctg agggggtagc
    43981 ccatttctta tacctcaaaa aacgttcttc tggcaatccc acccagcaat ttgcgagttc
    44041 ctttcctgac cttttttctt cgatcaaaac gggaatatct tcttttctgt tgatccatct
    44101 agataactcg attggctctt cattgatcag cttccatttt ttacaagaaa gaccctcttg
    44161 ataagtatat tttgctggag aatattctac cggatgtcct gattgatcga taatcacata
    44221 actttgatct tcaaaaacca gagctaaaaa aagcaattta ctttttcttg actgcagctg
    44281 ctgagtgctg gctaattttt taattttttt atctactttg aaatataaag gtttatccac
    44341 tacttgattc agttgggatc gcctcctttt tttcattata agtgaaggat tgaaaaaaac
    44401 aaatcagttt gattgttcga attttcaatt ttttatgttc tatttgttaa ttccttcatt
    44461 ttgtaagata atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa caaagaacga
    44521 ttgaaacaag atttctatga agcagtcaat gaagaatggc aaaaaacagc aaaaatccct
    44581 gcagataaac cagctacagg cggtttccaa gatttagttg acgggatcga ccaacttttg
    44641 atgggagaaa cagacgatct gcttgcccat ccagaaaaag tgtcttcaga aaaaatgaaa
    44701 catttccttg catattatca actggcaaac gattatgacc gaagaaatga gttgcatgct
    44761 caaccgcttc aacctataat cgaaaaagtt gaatcattgc agtctttcga cgaattgaat
    44821 aagcaatttc cagaatggct gttggataca ttaccattgc catttaatct ggatgtagat
    44881 gcagatatga agaatgcgca aacgaacgcc ttctttgctt atccgccttc tttattcctt
    44941 ccagataaga cgtattatgc atcagaccat cccaacggtg cacaactaat aggcgttttc
    45001 tttgatatgg ctgttcagtt attagagctt gccggcaaag aaaaaagtca ggcagaagca
    45061 atcacggaac aagcagtgca atttgataaa ttgattgctc ctcatgtaaa aagtgcagaa
    45121 gaaaacgcag attacagcaa aatgtataat ccgcaatctt ttgatacatt tattaaatac
    45181 acagataaac tggacttgaa aaaacttgtc acgggtttga ttgggacaac tccagataaa
    45241 gtgatcgtaa ctgatccagt gtacttcgat catttgaatg acatcctgac agaagctcat
    45301 tttccgttat tcaaaagctg gatgatcatt aaaacagtac gttctcttag tggttatcta
    45361 tcagaagaat tccgccaagt cagcggaatc tattctcgcg tattgtctgg aacagacgaa
    45421 gcaatgcctc agaaaaaagc agcgtattac cttgcctctg gacaattcga tcaagtagtt
    45481 ggcgattact acggaaaaaa atattttggc gaagctgcta aaaaagacgt agaacaaatg
    45541 gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg gcttggggat
    45601 gcaacaaaag aaaaagccgt cataaaatta aataacttag gtatccaagt aggctacccc
    45661 gataagatcc cagcattatt cgaccagttc aaaacagtac ctgcagatca gggtggtact
    45721 ttgctttcaa atactcttgt tttcagcaaa ctcacattaa aagatcgttt cagcaaatgg
    45781 aacaaaccag tggatcgttc agaatgggaa atgagtgctg acacggtcaa tgcttattat
    45841 catccatttc ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc attttacagt
    45901 ctagaacaat caagctctgc taattatggt ggaatcggag ctgttatcgc tcatgagatt
    45961 tcccatgcat ttgataataa cggcgcttta tttgacgaat atggcaactt gaacaactgg
    46021 tggacagaag aagatcttac acaatttcaa aacaaagcac aagcaatgat cgaagaattc
    46081 gatggtctgc cttttgctga cggaaaagta aacggaaaat taaccgtttc ggaaaatatt
    46141 gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc agatctttct
    46201 atcgaggaat tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa aaaagaatac
    46261 caacaacttt tactgcaaat cgatgtccat gcccctgcta aactccgagc aaacgtgcaa
    46321 ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca cggaacaaga cgctatgtat
    46381 ctagcgccag aaaaaagagt gcgaatctgg taagaggttg tgccagaagt ggtcagcttc
    46441 aagaaataag aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat ttcggcttat
    46501 ttttcgaaga agttacttct ggaataccat gtattagagg ttgtgaaaaa gctgcttagt
    46561 ttcaagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttgact
    46621 tactaccgag aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa tcagcgtcct
    46681 gacctgagta ttagagaaaa aaacggaaaa atagttcctc atattttgcc gtttgttgag
    46741 ctaataccga gggtcagctg attgaacacc gtttagtgga gagattgtga aaaagctgtc
    46801 ctgcatcaag taataagaac agccaatcaa aagcagcaag gagaacctca tctataaaaa
    46861 gatagaagga agtgagacat ctattggtct catttccttc tatttttact ttcctcagtc
    46921 aattgtttta catcataaaa ggagaaattc cacttatttt tttcgctgtt tcatgacata
    46981 aacaactaca ccgattaaca aaattcctcc agctaatgct agcaacgggt tattaaattc
    47041 tcccgttttt gggagtcctt gagtgccatt tttttcactt tcttttgctg tcctatccgg
    47101 atgagaaggt tttccatttg gttttccatt tggtgattca tttcctggat tttctctttc
    47161 tggtttgtca tttcccgggg gcacatcggc agtttctcgc acagtgatcg ctttttgctg
    47221 ggtaacagat tctgtatgag cagaccattt ttccccatca aatatttccg aacgataagt
    47281 gacttctagc gtatattctc cttttgaagg tgctgaaaaa atagcggtat gattagaaaa
    47341 ttgagcactt gactgtgttt caacgatttg gatcttttct gggaaataac gcatatcttc
    47401 aaagacaggc gttccttgat ttggtgtgaa agttgacaca aaaactgtaa aaggcttcag
    47461 ttcaatgata tttccttctg cattctccgc cacaccattt gcttcttgta taataatcgg
    47521 attattttgt tcatttggtt ttgtctgatt tggttcttgt acgattgcat actctggata
    47581 ttccgaagac ggtgaggttt ctccgtcaaa aatagcacct acagataatg gaagaccatc
    47641 cactctttcc ttttctgttt gacgattccc cggtttaatc tgcatcagtc catgaacatg
    47701 gatacttcca ttttcatagc gcgtaacagg ctgctgacct tctttcgaac atgtatttag
    47761 agactcgatc caatcttttg atatggtatg tccaagagta ttttctgatt gtttctctcg
    47821 ttcattccaa tagtagttca acgagtggta gatttgatct tcgttttgat tcaatgggcg
    47881 gatctgctct ctcatctcaa gattctctgt tcggaaagat aagataccag aagcatggta
    47941 gtctgtgttt cctgctgaag aggtataaag agccacatta tatgaaccgt tattaaaact
    48001 tgtactgcta ttgacgataa tatctgttcc gctattggaa tcgattcctt tcgcaagatt
    48061 ttcgaaagac aaactattga tcaattcatg agggcccgtt aaagaagaac cgcccatttt
    48121 gaatccatta ccgttacctt ctccctcaat tcctggtacc cagccgtttc gataagcaac
    48181 tgagtttttg atgattactt ttccgatcga acctgtttca tttttagcaa aaaggtccca
    48241 accgtcatct gcattatgat acgcgataca accgtcaaat acatttcctt ctcctattgt
    48301 cagtttggcc gcaaatccat cggcatcttc aaatcctgga tcggcatttt caaaagatgt
    48361 acagttcttg actaagttat gcgcgggcca tgcttcgaaa ggatcatttg agctaccgct
    48421 gatttggatt cctgtatttc cattccgata agcatgaatc tcttcgatca aattatgatt
    48481 tccagaaatc tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa accctcgtaa
    48541 accccagtag tgactgaaca gtgtcatccc atttccagtt cctttaaaat caagtacagg
    48601 acgcttgttt tctggatttt tctctgcaat caactgaatc gggttttctc ttgtcccgtt
    48661 aacgcctttt ggaatcgtca atcctctttc gaaggaataa gttccaggcg ccatgacgat
    48721 tgtctgccca gcatatgcgt atctcaatgc ttgtgtcaga gataatggat tttgtctgct
    48781 tccgtttcct gcatctgacc cttctggtgt tacataaact gtcgaatatt taggtttacg
    48841 gtagtccact gtatgaagaa tctcttttgt ttcataacta gtcatctcta cgtattcttc
    48901 tggcggatag tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc cttccatcag
    48961 ttttgtctga taatcaaatt cccgattcgc agtgatagcc aagttttttg cgttcttaag
    49021 cacctctcct gttgctttat ttttaattgt caataatccg tcagcatttg ctctaaagga
    49081 catagtatga tcttggtttc ctgaatgatt actagaggtc acagtgtaaa tcggatcaat
    49141 caagcgagta ggtctttctt gcccaggagg atctgtcgca ggatctgagt aagcaacttc
    49201 gattccttct accttgatcg tcatattcct tgctgcagca aatcctgcat aaataaccga
    49261 ttcatctgaa gcgtataatt tttcccagtc atacatgatg tcagaagcca tttcttctcc
    49321 tgtttcagga tcgtaataat atgcctctaa accagtattc gtttttttca tctctacgtc
    49381 cagtttagta atcgagttcg tggccggaaa cagattccca tgaccgtttc cgaatatatt
    49441 ataagaacct gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg ttgtttccac
    49501 tgagacactc ccaggagctg gcgcagcttg cggatcttca gatgatattc ctgtgattgc
    49561 ccgtgtcccc aatccaagtc gcatcgtata tttgctgccg ctcgtgttga ccacttcttg
    49621 tctatccgag tcccagacat attcgatccg actgcctagt atggcaaatg aatttgaata
    49681 aaatgcctgc ccaaacaaat gattttttgg tactgcgtct cttaccatta aagcaaatcc
    49741 ttcttgggca ttcgtatagg tccaagattc tatttcgatc gtcgcccgca tcctaaagtt
    49801 gatgtctttg gggattgctt gataataata ggtcattcca tctgatccgc ttgactggaa
    49861 ttttccgccg ttattcgtgg aagagagccg cacgccgtct tctaatggtt caatggcata
    49921 cctactagca cttgtgcttt ctccgaaaac aacccctttc cactcttcat ttgcttcttc
    49981 agattgcttt tttgtctttt cattttctga ttctttcatc ataggatcat ttccttcttc
    50041 cgtatccata tcttcactag ttcttggtat ttcctccatt gttgacatcc cttcaatgtt
    50101 cacgtgtgca ttttcttcgt tcacagactg ttgttcgtta gcagcaactt caacgacaga
    50161 aattaaaacc ggggtactga gcataaggag attcatcatt agcgcactgt attttatacg
    50221 tttttttaac aagtgtattc ctcccgtaac aaattagaaa gcgtttactt aaagtattac
    50281 ctaatttatt tttactgtca ataaaaaaca aaattataat aagtgtattt tataaaaaga
    50341 aaaaatataa cttaagttgt tgataataaa gagatttatt aacgtaaaaa gaataagttt
    50401 ttttaaaata acgtataaaa aagagcataa aacaaagtcg tttgttttat gctcttcttt
    50461 tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa tttctgcgcc
    50521 tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga aacttgccac
    50581 ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg ctgtatgcaa
    50641 cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc gttctcgcaa
    50701 acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta tccaataaac
    50761 attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca attcccgtcc
    50821 acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa aggaatcttt
    50881 tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa acttctttgt
    50941 atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca gctttccatc
    51001 ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct tagtcctcct
    51061 tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg
    51121 ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg
    51181 atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc ttccatttcg
    51241 caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg cgagtcgaca
    51301 aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc ttttgctgct
    51361 ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat tcctggtaat
    51421 tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac tttgtctgat
    51481 ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc tgtattgatc
    51541 accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc taagactttt
    51601 cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga aataaataaa
    51661 gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat ttttacttct
    51721 tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt ctatataaat
    51781 gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga tttcatcaaa
    51841 aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt gattggttta
    51901 ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg ctgataagct
    51961 gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga
    52021 agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta ctttcctttg
    52081 gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat caactgatct
    52141 ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac ttcgtcttca
    52201 tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat atgaaggact
    52261 tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga cttcgcacga
    52321 tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg tccttctcct
    52381 ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt tcgaaattgt
    52441 ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc gccaggatga
    52501 aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc tacagcaaca
    52561 tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc aaattgctca
    52621 ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt ttcatttttc
    52681 aatttttctc aaaaactcct tattagttta ccatagataa accttagatt ttctctaaat
    52741 atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta aaataagtat
    52801 gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag ggtttattat
    52861 gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct ttgtccgaaa
    52921 caatagtttt tcttttgttc taggactttt attgataggt gtagttttag ttttgtgggg
    52981 actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac tgactgacga
    53041 acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat tttttcgaca
    53101 aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc agcagacagc
    53161 caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag ctttattcaa
    53221 agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt atacgcatct
    53281 gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg agatcaaaaa
    53341 caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt
    53401 aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg acgtagagat
    53461 atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac aaaaggatta
    53521 actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct gtttcgagta
    53581 ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca aggaggaatt
    53641 ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg atacacttga
    53701 agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt cacaacaagc
    53761 ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca aactaccggc
    53821 ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg atgcacagtc
    53881 tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac aatcgatgtt
    53941 gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg aactgatgta
    54001 tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca tcttccagcg
    54061 tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg
    54121 tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc tgctaaaaga
    54181 taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg cactgaatat
    54241 ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca ttccagaagc
    54301 gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca atgattacac
    54361 gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta gacagatcac
    54421 gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa
    54481 gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt
    54541 gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg aaacaacaaa
    54601 cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga
    54661 aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg atttgatcga
    54721 aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga
    54781 agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt taacccaata
    54841 gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac aactgttgat
    54901 gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc attcatcttg
    54961 ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac gataccgaac
    55021 acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa aatggagacg
    55081 attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt gccaaacatt
    55141 tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc catgatattc
    55201 gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc tgccacacca
    55261 gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt cataaactta
    55321 ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata catcatcgag
    55381 atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc cactgttcca
    55441 tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc agtatacgga
    55501 acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa
    55561 ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc tgccattgca
    55621 acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag cacaccggaa
    55681 aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg
    55741 ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa acgtaatgct
    55801 ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac cgcaatcaag
    55861 tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg aagttcggcc
    55921 cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac agaggaaacc
    55981 gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat
    56041 acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc aaattctgcg
    56101 aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag tgacagtgct
    56161 ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt accgatgatc
    56221 gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat ctcactcgta
    56281 tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc ccattttagg
    56341 ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc taaaatgact
    56401 aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc gccaaggatg
    56461 aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa gccaacagct
    56521 aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca taataatcca
    56581 cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc aaagaagctt
    56641 gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc caaattcgcc
    56701 cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa tgctctaaaa
    56761 cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc atcttgtcgg
    56821 gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta tacataatct
    56881 ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa aattaattga
    56941 aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc atggaactgt
    57001 ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa aatccaaggg
    57061 gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc accgatcgtc
    57121 attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa accaatacta
    57181 ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct ccttttattt
    57241 cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt tttcgtttct
    57301 aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata aagtcattgt
    57361 tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt cggccagatt
    57421 ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac catttctccc
    57481 agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga tccctgaatc
    57541 tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga tgtttccgtg
    57601 atccggcgta tatttgatgg cattactaag caattggttg aagataaaag cgatccattt
    57661 agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat ttttttggat
    57721 aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc catattcttg
    57781 gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt gttctacgta
    57841 ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt caatatcgaa
    57901 ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt catggaccca
    57961 tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca ttacttcttg
    58021 gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt ctgtcctccc
    58081 accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt ttccccacca
    58141 tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc cctcaatcaa
    58201 agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc acatcacaaa
    58261 gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat ctttcaaata
    58321 tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc ctgtcctact
    58381 tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa ccgattgatg
    58441 ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc tcgcagcaat
    58501 ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag acgatattca
    58561 tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc aacattcaac
    58621 gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta agaacggcga
    58681 agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt cacaaaatca
    58741 tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct ggagataaaa
    58801 atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc atccaaaacg
    58861 ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc tgagaacact
    58921 tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc ctccatgatc
    58981 aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa aaatccctcc
    59041 atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt ggcttacctg
    59101 gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta atcgcgtttg
    59161 tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta ttatctgtta
    59221 tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc gaaaagcgtt
    59281 agcactagca atatacctag aaggagtata agtaatcgat aaagatggta tttttttgtc
    59341 tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt cactagtaat
    59401 cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc ctgcttttct
    59461 ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac gacttcataa
    59521 gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat ttcttcaaga
    59581 tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga atgtgtactc
    59641 tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc taacaaaaga
    59701 tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata gatggctccg
    59761 atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac cgcttgctca
    59821 aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag cgtctgattt
    59881 ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg ttcaagctgc
    59941 ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc tgctaaaaat
    60001 ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa ttgtaataaa
    60061 agactgattt gggtttgagg tctattacct attttctttt tcattttctt caccttctta
    60121 tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt ttgttgctta
    60181 tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca tcaaacttat
    60241 tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg acccgcgttt
    60301 catattcggt actctttctg gccctggatt ttctactcct acgatctgaa tttcttcatt
    60361 atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg gaagtacata
    60421 accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc cttgaacctc
    60481 aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg cccactcaat
    60541 tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa atattgcgta
    60601 ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt cactgcttga
    60661 tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg gctctactcc
    60721 ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt tgttatggaa
    60781 atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta aatctcctcc
    60841 acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt cttctgtcaa
    60901 aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc cttttgttaa
    60961 aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac caactgtagg
    61021 cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga cagaatcgat
    61081 gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt ctagatcaat
    61141 ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc taaggtcgct
    61201 ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat acaaatgcac
    61261 atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca ccatatcagg
    61321 catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat gagctggcga
    61381 ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt ccgtcacttg
    61441 gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa tacttgtcag
    61501 tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa tcgcttcggc
    61561 agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca aatcgtaata
    61621 tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt caccaaaatc
    61681 cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac cttttttgtg
    61741 taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga ctagttttcc
    61801 gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc ccgtgacaat
    61861 cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca gcacctaaca
    61921 ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc gggaatacgg
    61981 accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt ttgtccgttg
    62041 tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca gagactggaa
    62101 ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat tgtggttggc
    62161 cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt gcttcatcca
    62221 tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa ggcaagaaac
    62281 gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca tttttgtcat
    62341 tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta gcaggagcca
    62401 tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca gcaccttcta
    62461 atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag tatggatctc
    62521 ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg gttgattctg
    62581 tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg atatcaacat
    62641 ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca tcccgatctt
    62701 ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca accgtatctt
    62761 tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt agctgacgga
    62821 tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc atgttttcag
    62881 cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc gctccattta
    62941 gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag atagccaagc
    63001 catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc atccgtttat
    63061 gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca tgtggaagaa
    63121 ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac agctttttca
    63181 ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg ctcctgcaag
    63241 cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc ctgtagcagg
    63301 caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat ttgtctccaa
    63361 aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt ttggataata
    63421 cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat cattttctat
    63481 gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt ttctatagct
    63541 gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg aagcattgaa
    63601 tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat actctcctgc
    63661 actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat tcatactgtc
    63721 aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta cttgtagtac
    63781 accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga tgaccgtatc
    63841 ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat cagatagccg
    63901 atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca gatgcagagg
    63961 ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat agaagtatcc
    64021 gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt atgctcgata
    64081 cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat ctaatgtatg
    64141 gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat tgtaagcagt
    64201 gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt gccaattttc
    64261 ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc gcaagatcca
    64321 tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa tgattttgta
    64381 ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg gctctccgat
    64441 tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat ctactgaata
    64501 gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc ctttcgctac
    64561 tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact ctgcgccatt
    64621 gattggcata ttactgcctt caattagttt attgatattc atgtggattg gcttgaattc
    64681 atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta tgttaggctc
    64741 ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat ctttaccttc
    64801 cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt gacctaacat
    64861 ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat ctactaactg
    64921 ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg ctccagtttc
    64981 atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg tagttttact
    65041 tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg caaattcgac
    65101 tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac ggcctttatg
    65161 ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat cattctcata
    65221 atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc cgtcttgagc
    65281 tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac ctaaagaaat
    65341 cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag tcgccactac
    65401 ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt ggtacttcat
    65461 gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc cagctccaaa
    65521 tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg ttctcttgaa
    65581 ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg cattgtaata
    65641 tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat ttcgtccaat
    65701 agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa tggagaagat
    65761 acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa ctgtgttgat
    65821 cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag aagaaccatc
    65881 accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac gatagtgtac
    65941 atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat gagtaaaccc
    66001 tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt cattatcttc
    66061 agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc tcgtacgata
    66121 acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa cgattaggat
    66181 tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat agccattttt
    66241 aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg tattctcact
    66301 tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga tttgctcatg
    66361 gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa tatccagact
    66421 cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg cagcattttt
    66481 agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat ctagaacaaa
    66541 aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta gatagacatt
    66601 gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg gcagaacagg
    66661 ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt caaaattccg
    66721 ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt cgcctgcagt
    66781 tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt ttgtataatc
    66841 actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt gatagcgttc
    66901 attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa acttcgggta
    66961 gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg ggtcttgcgt
    67021 agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa tgcgctggag
    67081 tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt cttcgctacg
    67141 ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt ggttatctat
    67201 tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg ggatcttttc
    67261 tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc cgacatacca
    67321 ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat aggaggatag
    67381 acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat cgattgctaa
    67441 acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc attcaagggt
    67501 ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta agaatacctc
    67561 atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat gatagactga
    67621 ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct tcaattttaa
    67681 ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa caatcaacaa
    67741 aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa tgatatcctt
    67801 tttagttaaa aaacaccaat tatacaacat ttattattaa aattaaatta taaaatttta
    67861 taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca actacgcttt
    67921 cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt tattcgtaca
    67981 attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa tagctcctcc
    68041 taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga tcaaacaagc
    68101 aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag ctactgataa
    68161 aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac ttgtgtaaag
    68221 gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt tgataaaaag
    68281 caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat attctctagt
    68341 atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac cattagagat
    68401 attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt gcttggcgaa
    68461 cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat tcctcctttt
    68521 taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa aacaagaaac
    68581 tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca agaaggatca
    68641 acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag aaaaatgcag
    68701 taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta aagagaagcg
    68761 cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt cccccacccc
    68821 aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt gctaaactat
    68881 cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt gaaccgagct
    68941 ttttctcttc attttttttg taatgataaa tcatatacag aactgttcga cttgcagttc
    69001 ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg ataagacgtg
    69061 gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc gatcgattca
    69121 tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt gtgcaaaaaa
    69181 gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat aattccattg
    69241 tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt tttcttgatc
    69301 ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg aattgatcgc
    69361 aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa tatacaaatg
    69421 attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata agtaaaattg
    69481 acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca ggggcaaata
    69541 agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg ttacaaaccc
    69601 cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag gaaaatcaag
    69661 cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca ctactagcac
    69721 agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa tcgtatccag
    69781 atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa cgatttcttc
    69841 cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa tgattcctat
    69901 tactgtcatg aggacataac ttagtatttt ttttgtttgc cgattcacac tggtcttttt
    69961 gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg tgcaagcttc
    70021 aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat agccatcgca
    70081 taacccatat caaaagctac gaacgccttg ttccacatat gcagattata aaaaagcaaa
    70141 gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg aaaaatttgg
    70201 aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat taatggcaaa
    70261 gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga ttcatataaa
    70321 gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc agaactccat
    70381 attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca agctggtcca
    70441 gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga aagcagctgc
    70501 atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa agctgtacgg
    70561 aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac actgaaaatc
    70621 gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga tttccaaaat
    70681 aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt catcttggat
    70741 gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc tagcataaat
    70801 accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc ttcttttttc
    70861 tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac agtcaaaaat
    70921 ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg ccaatccttc
    70981 ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta atgtcgtgta
    71041 atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt cgtccgccat
    71101 ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat tctcattggc
    71161 tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta cttctggaga
    71221 agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt atggtacttc
    71281 caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt ctgggagctg
    71341 catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt aataattgat
    71401 attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac cagacccaaa
    71461 ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt cttggatcca
    71521 ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt tgtcaaacca
    71581 actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc ataatggata
    71641 aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg ctgtttcttc
    71701 taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt taaataactt
    71761 tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag gatcaccttc
    71821 atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt tttctacata
    71881 tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat ttatgtcctg
    71941 tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag tccagatgtc
    72001 accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat tgaaatcctc
    72061 aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc agaaatcaat
    72121 attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac tggcatcgct
    72181 tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca gcaaccctgt
    72241 aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc cggttttaat
    72301 ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct aaaaaaatta
    72361 ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt ttaatctttc
    72421 gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt tagacaccag
    72481 aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc gaaatatctg
    72541 caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta cctgttccaa
    72601 ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat tgcaacagaa
    72661 gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga gggtgcgcct
    72721 gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat tatctctgga
    72781 atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga cagcgtgggc
    72841 aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga ctttagagct
    72901 aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga aatgatcgat
    72961 gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta tcatcaaaat
    73021 ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt agtcccaagt
    73081 actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa cttctcagag
    73141 ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg cgaaggcgaa
    73201 tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga accagtaaca
    73261 ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag aaacacccgt
    73321 tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat aaccaccctt
    73381 caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc ctaaagacgc
    73441 tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc acctcttaaa
    73501 aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta cactcagctt
    73561 atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg tcgcttcatt
    73621 ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc caaatttata
    73681 ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat tgattataaa
    73741 ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc tactttgtac
    73801 gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg tatttcaatc
    73861 gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg cttcactgac
    73921 agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg caatctattt
    73981 tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa taaag
//
LOCUS       DACDHL010000004        72530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDHL010000004 DACDHL010000000
VERSION     DACDHL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.004"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..209)
                     /locus_tag="IV228_000271"
     CDS             complement(<1..209)
                     /locus_tag="IV228_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716220.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSE"
     gene            complement(384..2519)
                     /locus_tag="IV228_000272"
     CDS             complement(384..2519)
                     /locus_tag="IV228_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8716221.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2602..4257)
                     /locus_tag="IV228_000273"
     CDS             complement(2602..4257)
                     /locus_tag="IV228_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8716222.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4259..4573)
                     /locus_tag="IV228_000274"
     CDS             complement(4259..4573)
                     /locus_tag="IV228_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8716223.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4743..5003
                     /locus_tag="IV228_000275"
     CDS             4743..5003
                     /locus_tag="IV228_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8716224.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5017..5331
                     /locus_tag="IV228_000276"
     CDS             5017..5331
                     /locus_tag="IV228_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8716225.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(6061..6615)
                     /locus_tag="IV228_000277"
     CDS             complement(6061..6615)
                     /locus_tag="IV228_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8716226.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6741..8670)
                     /locus_tag="IV228_000278"
                     /pseudo
     CDS             complement(6741..8670)
                     /locus_tag="IV228_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(8976..9692)
                     /locus_tag="IV228_000279"
     CDS             complement(8976..9692)
                     /locus_tag="IV228_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8716227.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9693..11516)
                     /gene="glpO"
                     /locus_tag="IV228_000280"
     CDS             complement(9693..11516)
                     /gene="glpO"
                     /locus_tag="IV228_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8716228.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11530..13026)
                     /gene="glpK"
                     /locus_tag="IV228_000281"
     CDS             complement(11530..13026)
                     /gene="glpK"
                     /locus_tag="IV228_000281"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8716229.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13162..14622)
                     /locus_tag="IV228_000282"
     CDS             complement(13162..14622)
                     /locus_tag="IV228_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8716230.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14612..15907)
                     /locus_tag="IV228_000283"
     CDS             complement(14612..15907)
                     /locus_tag="IV228_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8716231.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16239..16916)
                     /locus_tag="IV228_000284"
     CDS             complement(16239..16916)
                     /locus_tag="IV228_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8716232.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16946..18037)
                     /locus_tag="IV228_000285"
     CDS             complement(16946..18037)
                     /locus_tag="IV228_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8716233.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18138..18572)
                     /locus_tag="IV228_000286"
     CDS             complement(18138..18572)
                     /locus_tag="IV228_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8716234.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19156..20613)
                     /gene="ascB"
                     /locus_tag="IV228_000287"
     CDS             complement(19156..20613)
                     /gene="ascB"
                     /locus_tag="IV228_000287"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8716235.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20610..22505)
                     /locus_tag="IV228_000288"
     CDS             complement(20610..22505)
                     /locus_tag="IV228_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8716236.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22563..24338)
                     /locus_tag="IV228_000289"
     CDS             complement(22563..24338)
                     /locus_tag="IV228_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8716237.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24388..25884)
                     /locus_tag="IV228_000290"
     CDS             complement(24388..25884)
                     /locus_tag="IV228_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8716238.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26078..27700)
                     /locus_tag="IV228_000291"
     CDS             complement(26078..27700)
                     /locus_tag="IV228_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8716239.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28028..29341)
                     /locus_tag="IV228_000292"
     CDS             complement(28028..29341)
                     /locus_tag="IV228_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8716240.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29586..29810)
                     /locus_tag="IV228_000293"
     CDS             complement(29586..29810)
                     /locus_tag="IV228_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716241.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29830..30198)
                     /locus_tag="IV228_000294"
     CDS             complement(29830..30198)
                     /locus_tag="IV228_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8716242.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(30394..31104)
                     /locus_tag="IV228_000295"
     CDS             complement(30394..31104)
                     /locus_tag="IV228_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716243.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31122..31619)
                     /locus_tag="IV228_000296"
     CDS             complement(31122..31619)
                     /locus_tag="IV228_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716244.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31656..32108)
                     /locus_tag="IV228_000297"
     CDS             complement(31656..32108)
                     /locus_tag="IV228_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8716245.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32243..32848)
                     /locus_tag="IV228_000298"
     CDS             complement(32243..32848)
                     /locus_tag="IV228_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8716246.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33298..33918)
                     /locus_tag="IV228_000299"
     CDS             complement(33298..33918)
                     /locus_tag="IV228_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8716247.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33920..34789)
                     /locus_tag="IV228_000300"
     CDS             complement(33920..34789)
                     /locus_tag="IV228_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8716248.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34812..35219)
                     /locus_tag="IV228_000301"
     CDS             complement(34812..35219)
                     /locus_tag="IV228_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8716249.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35238..36527)
                     /locus_tag="IV228_000302"
     CDS             complement(35238..36527)
                     /locus_tag="IV228_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8716250.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36554..36856)
                     /locus_tag="IV228_000303"
     CDS             complement(36554..36856)
                     /locus_tag="IV228_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8716251.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(36881..38974)
                     /locus_tag="IV228_000304"
     CDS             complement(36881..38974)
                     /locus_tag="IV228_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8716252.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(39463..40227)
                     /locus_tag="IV228_000305"
     CDS             complement(39463..40227)
                     /locus_tag="IV228_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8716253.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40338..41207)
                     /gene="mdcB"
                     /locus_tag="IV228_000306"
     CDS             complement(40338..41207)
                     /gene="mdcB"
                     /locus_tag="IV228_000306"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8716254.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(41232..42308)
                     /locus_tag="IV228_000307"
     CDS             complement(41232..42308)
                     /locus_tag="IV228_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8716255.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(42332..42925)
                     /locus_tag="IV228_000308"
     CDS             complement(42332..42925)
                     /locus_tag="IV228_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8716256.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(42929..44605)
                     /gene="mdcD"
                     /locus_tag="IV228_000309"
     CDS             complement(42929..44605)
                     /gene="mdcD"
                     /locus_tag="IV228_000309"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8716257.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(44595..44894)
                     /locus_tag="IV228_000310"
     CDS             complement(44595..44894)
                     /locus_tag="IV228_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8716258.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(44996..46642)
                     /gene="mdcA"
                     /locus_tag="IV228_000311"
     CDS             complement(44996..46642)
                     /gene="mdcA"
                     /locus_tag="IV228_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8716259.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(46649..47560)
                     /locus_tag="IV228_000312"
     CDS             complement(46649..47560)
                     /locus_tag="IV228_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8716260.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            47680..48537
                     /locus_tag="IV228_000313"
     CDS             47680..48537
                     /locus_tag="IV228_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8716261.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(48724..49086)
                     /locus_tag="IV228_000314"
     CDS             complement(48724..49086)
                     /locus_tag="IV228_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716262.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            49432..50871
                     /locus_tag="IV228_000315"
     CDS             49432..50871
                     /locus_tag="IV228_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8716263.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(50878..53430)
                     /locus_tag="IV228_000316"
     CDS             complement(50878..53430)
                     /locus_tag="IV228_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8716264.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(53544..54875)
                     /locus_tag="IV228_000317"
     CDS             complement(53544..54875)
                     /locus_tag="IV228_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8716265.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(54841..56319)
                     /locus_tag="IV228_000318"
     CDS             complement(54841..56319)
                     /locus_tag="IV228_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8716266.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(56624..58459)
                     /gene="tdc"
                     /locus_tag="IV228_000319"
     CDS             complement(56624..58459)
                     /gene="tdc"
                     /locus_tag="IV228_000319"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8716267.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            58929..60356
                     /locus_tag="IV228_000320"
     CDS             58929..60356
                     /locus_tag="IV228_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8716268.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(60448..60813)
                     /locus_tag="IV228_000321"
     CDS             complement(60448..60813)
                     /locus_tag="IV228_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8716269.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(60810..61175)
                     /locus_tag="IV228_000322"
     CDS             complement(60810..61175)
                     /locus_tag="IV228_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8716270.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(61367..61615)
                     /locus_tag="IV228_000323"
     CDS             complement(61367..61615)
                     /locus_tag="IV228_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716271.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            61614..61742
                     /locus_tag="IV228_000324"
     CDS             61614..61742
                     /locus_tag="IV228_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8716272.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            61799..63493
                     /locus_tag="IV228_000325"
     CDS             61799..63493
                     /locus_tag="IV228_000325"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8716273.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            63497..63616
                     /locus_tag="IV228_000326"
                     /pseudo
     CDS             63497..63616
                     /locus_tag="IV228_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            63698..63889
                     /locus_tag="IV228_000327"
     CDS             63698..63889
                     /locus_tag="IV228_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716274.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            64267..65400
                     /locus_tag="IV228_000328"
     CDS             64267..65400
                     /locus_tag="IV228_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8716275.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            65431..65751
                     /locus_tag="IV228_000329"
     CDS             65431..65751
                     /locus_tag="IV228_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716276.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(65780..66244)
                     /locus_tag="IV228_000330"
     CDS             complement(65780..66244)
                     /locus_tag="IV228_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716277.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(66375..67628)
                     /locus_tag="IV228_000331"
     CDS             complement(66375..67628)
                     /locus_tag="IV228_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8716278.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(67657..67974)
                     /locus_tag="IV228_000332"
     CDS             complement(67657..67974)
                     /locus_tag="IV228_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8716279.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(68000..68320)
                     /locus_tag="IV228_000333"
     CDS             complement(68000..68320)
                     /locus_tag="IV228_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8716280.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(68784..70199)
                     /locus_tag="IV228_000334"
     CDS             complement(68784..70199)
                     /locus_tag="IV228_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8716281.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(70216..71532)
                     /locus_tag="IV228_000335"
     CDS             complement(70216..71532)
                     /locus_tag="IV228_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8716282.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(71682..72380)
                     /locus_tag="IV228_000336"
     CDS             complement(71682..72380)
                     /locus_tag="IV228_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8716283.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT        21247 a        15365 c        12247 g        23671 t
ORIGIN      
        1 ttttctgata aaaaagcgta caaggaaaaa tccttggctc tttctggtct cgttaatggg
       61 aaaaagaccc atataaaagg aagaacaacg atcatccatc ctaaaagagc cactaacgtt
      121 tgccaaatct ttaaccagta attacctttt gaaaaatact tatctttcac caaaattgat
      181 gcaacttctt ctcttgttac ttttttcata tcctcacctc acgttttttt gttacgacgt
      241 aggagtttac catacatttc atttcttttc acataaagtg ttcctaaatt tttcctaaga
      301 aaaagaacaa aacatttctc atcagcaaat gaaaaatatt ttgttctttt tatcagttgt
      361 tctttttatt ttttatctct taattatgtt gctctttcca atacgacaat gcttttcatt
      421 cctctagata tcgccgtgta catttgtctg cgtcttcttt gctcatctcc atcgttttca
      481 aatggatata agattacatg atcaaattcc agtccttttg ctagactcaa aggaatgatt
      541 tggcagtttt ctaatccata tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc
      601 tcttctgttt gggtgataac tgccgctttt tctcctttta gagaaaccaa aatctcagct
      661 agtattttca acaattcttc agaggatcga aaagagcggt attcgggttc ttttccttgc
      721 ggtcggatcg atacgatgtc gatcttctct tgatccacag cataggtttt gaaaagctct
      781 gtgattgctc cgctagaacg gtaactattt ctaagatcat accgatggat cggcagatgg
      841 tagtcgtcga aacaacgcat aatatcagca aaagtagtgg aagaattaaa gattgcctga
      901 ttctcgtcac ctactaaagt aaagtgagtt tttggaaata attcgatcaa tagacctaat
      961 tgtgccaggg tataatcttg tacttcatca atcaatagat agcgaaatgc tggtacattt
     1021 accttttcaa caagtaaatg acgaatagct aatataatga ctgcttcatc aacagtacgt
     1081 gtgggttgcc aagcataagc tgagccatat cgtttttcat aaattctttc aaacaactga
     1141 tgctcatcca cccatgccat ttcttcgatt tgtctggtta tttttcgata ttttttttgt
     1201 aaaagtttcc ttgcataagc tacaatactt tgttccgaat catcagaaat caatttaccg
     1261 aataattcca actgcctatc ttctgtcaac gagagtacct gattctgcac agcaggactt
     1321 tttgcttgtt tcaaaagatg attcttccat tcgctcatca gtgccttttt cgttgcttga
     1381 agcctatcga tcaatttagg atgagcagga actttctcat atattttttc gatcgttttt
     1441 ttagaaagga ctgtttttcc atttttagta agtccacgga taaaattcgg atgatctaaa
     1501 aacaaaggat ctgattgttt tagattatcg ataaactttt tgctacgcaa acgttctgtc
     1561 tgctccgata cattttctcc cgtgatacgt ttgaaataag cctcttcacc ttctatttcc
     1621 tctgctgaaa gctgtgaaac cagctgtatg atggttagat tcagtggatt tctttcacct
     1681 aatgatggta ggacattcga aatatagtcg ataaaccgat gattaggtga tagaatcagc
     1741 aagtcatcgg atgtcatcgt atctcgatat aaatagagca gatacgcaat acgctgcatg
     1801 atagcagagg ttttaccact tcctgctacg ccatttacca agatattttg acttttcaca
     1861 tcccgaatga ttgtgttttg ttccgattgg atcgtagcag tgatagcctt catttcattg
     1921 gtctcatctt ggcctaagac ttctaataat acgtcatctt gaatagctac agacgtatca
     1981 aaaaacttga ccaaacgatc tctttccaaa agaaattgtc gtctttcttt aataaccgta
     2041 tcgattttat tttgacgaac cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt
     2101 tcggcaattg gtgaacgcca atcataaaca agattttcat ctgcatcatc tgtgaaattt
     2161 gctgtaccga tataaaagga ttcttttggc tcattatcca gaaagtctac aacgactttt
     2221 ccaaagtagg gacttttcaa taaacgttcg atctttgcta gctgattttc tgcgtttttc
     2281 tgtttgatat tcatctgatc gatttctcta tttttcattt caatcatcga aaaagtatcc
     2341 aggttatcta agtaactgtc aaaattcaat cgaagctcgc cagccatttc tttcaacgac
     2401 tggtttcctt ctatttttgc cgtatccatc gattcccgca gttcgttttt tttatcaatt
     2461 agcttttgat acacgttctt cagatatgct tcttctaact gtttttgtgt gttgaccata
     2521 cacattctcc tcttttttta aagtggtgga accgattagt ttacctcttt taagagtcga
     2581 atgcaagctt tttaacgagt atcatttgtt aggatatatc agttcttcag gtcggacagt
     2641 ttgatataaa gaaaatgcac catcaaggtt ttttacgtgg tagccagact gcttcaagat
     2701 tctttccgct agatagctac gtaatccgct atgacagctg acgatatatt cttgttggct
     2761 gtctaattca tttaatcggt cccgaagttc attcaaaggg atatgtttgg cttccttgaa
     2821 tttgcccttt tccaattctt ctgctgttcg cacatctaat aaaatttttc cttttgctag
     2881 ttctgtcggc aattcatgcc attgtacggt ttcactaagc ccttctgcaa tgttcattgc
     2941 tgcatatcct agcatgttta ctggatcttt ggctgagcca aatggcggtg catacgtcaa
     3001 ttctaattcc ggtaaatcga agatcgtcaa atgccctttg attgctgttg ctaaaatatc
     3061 gatccgttta tccacacctt ttgccccaac accttgtgca ccataaatct cgccagtctc
     3121 tggatggaag atcaacttca gtaaaatatc tgttgcttct ggataatagc ttgcatgatc
     3181 ttttcctgat acatgaatga cttcaaaaga cagttcgttt tctttggctg ttctttcact
     3241 taaaccggtg gatgcggcag tcattccaaa cgttcgtata attgctgttc caataccgcc
     3301 ttgatttctt cgagaaattc cggaaatcgt gtccgcaact tgtctacctt gacgattggc
     3361 tggtgaagca agagaaataa gtgcatcttt tccagtgatt tgctgtttta caacgatagc
     3421 atcccccacc gcataaatat caggaatatt cgtttgataa cgttcgtcta ctaggatacc
     3481 cccgcgtatg cctaattcga ttccagcggt cactgctagc tggttatcag gacgaacacc
     3541 aatggataaa ataactaaat cagcagggat ggtacgccca tcatccagtc gcaatcgttt
     3601 cccgtggtct tcaaatccag cgacagcatg agaagtaatc acttctactc cttgatgaga
     3661 caattctttt tctataaaag ctgccatttc ttcatccagt gttggaagaa tatgtggtgc
     3721 ttgttcgatc accatgactt ctaaacctct tctttttagg ttttctgcca tttccaatcc
     3781 gatgaatcct gcaccaacga tcacggcacg ctttggctgt ttttctaatg catgcatcac
     3841 tttatcgata tctggtacat ttctaataga aaaaacattg tcggcttctg ttatccctgg
     3901 aatcgatgga acaactggtt ttgcaccagg agataaaacc aatgcatcgt actgttcaat
     3961 gtgtttttga ccctcatgta cgacttctac cgttttgttt tcgggaaaga tggctgtcac
     4021 ttcatgattt ggacgcacat ctaaacaaaa acgagcggat aatgattctg gcgtttggac
     4081 aagaagattt tcccgttcac tgatttctcc cgaaagataa taaggcaagc cgcaatttgc
     4141 aaaagaaaca tacggtcctt tttcaaaaac aacgatttct gcatcttcca ttaatcggcg
     4201 aagccgtgta gcagcagaca ttccacctgc tacacctccg acaataacga ttttcatctt
     4261 atttccctcc tactgttcga tcaaaccatt gattcatgcc accacgtaca ttcactacat
     4321 cataaccaga tttctttaat tctttagctg cttgtttact cctcatccct gattgacaaa
     4381 tcacatagac ggtcttttct ttgtctccat catatctgtt tatactttgt aatggaacat
     4441 ttttagctcc ttttatatgc ccgccccggt attctgcagg tgttctaaca tcaagtaacg
     4501 ctggattttc caaaagcttt tcctttaatt ctgggactgt tatcgatttt attgattgaa
     4561 atagcaaatt cattttctgc acctcattta catttttata aatacctcat ggggtatatt
     4621 aaacaggagc tttattaata tgtcaaccag aatgactctt catttttttc actacttttt
     4681 atatttgcat aaaataccga taaggggtat tatctaatta taaaaataga ggagaatgtg
     4741 ttatggagat tgataataaa aatatcctga accgtttgaa acgtacagag ggacaaattc
     4801 gtggtatcca aaaaatgatc gaagacgaaa aagaatgtat ggatgtcatc actcaattaa
     4861 gcgctgttcg ttctagtatt gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa
     4921 attgctttga gaaacctgat agtaatccag aagaacaagc ggctaaacta gaacaagcga
     4981 tcaagatgat tattaaaaag taaaggaatg atttcaatga aagtcgtttt ccatattgat
     5041 gaattagaaa aatggtcaga aacagggaaa aacgtaaaaa acctgataaa agcttctcca
     5101 gaaacgacga ttgtcgtatc tgtaaatggc atcgcgatta ccggttatct agactctgcc
     5161 aatgcagaat tcttagattt gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt
     5221 gctaatcata tttcagaaag ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc
     5281 cttgatttag ttgaactgca atcccaaggt tatgcttata tcaagcctta gtcagtagag
     5341 gttgcgacag aagtgatcag cttcaagaaa tttcggctta tttttcgaag aagctacttc
     5401 tggaaaaccg tgaatcaaag gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc
     5461 caaacaaaaa tagcttctca tatttttcgt ttggttgggc ttattaccgc agatgcaagc
     5521 ttttgaacac cgtttaagga agaggttgtg aaatcagcgg tttgacctaa gtattagaga
     5581 aaaaaacggg aaaatagctc ctcatatttt gccgtttttt gagctaatac cgaaggtcag
     5641 ctgattgaac accgtttaag gaagagattg tgaaaaagtc gcttagtttc gagtattaag
     5701 aaaaattttg tgaaaatgga tttctacatt tttgcataaa tttggcttaa taccgagaaa
     5761 ctggcttttg aataccgttt aaggaagagg ttgtaaaatc agcggtttga cctgagtatt
     5821 agagaaacaa acggaaaaat agctgctcat attttgccgt tttttgagct aataccgaag
     5881 gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca
     5941 caagaaataa tttcaaaaac aacttctcct gtttttggtt cggttggctt attatcgaga
     6001 agtagctact gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt
     6061 ttattttctt tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag
     6121 accgtaagtg atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag
     6181 cagcaaaagg atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc
     6241 tccacttaat ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc
     6301 ttttatcaag aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa
     6361 aatcagaaaa agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt
     6421 tacctgatta tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac
     6481 aatgaccgct cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag
     6541 caaaaaaata cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc
     6601 tgtgcttaat ttcattttat cgccctacct cctcagcact cttttctttt tattttagct
     6661 tacacgggga ataaaaccaa tgatttcact gtttttattt ttctttacag acactgatat
     6721 tatgagctaa tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt
     6781 cgtttgcgca tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca
     6841 cgctgccgtt tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg
     6901 tacagatctt ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt
     6961 ttgtctaaat atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt
     7021 tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctgacgttc ttttttattc
     7081 tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct
     7141 ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga
     7201 gcaatccaca aagcttggct ttctcctgtt tcttccaacc cgcaactgat atttaggacg
     7261 aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa tcttcatgat ctccggcgaa
     7321 tttcttgatt tctccataat gggtcgtggc aatgatcaat gccccttgct catacataga
     7381 ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc tctgttccac tgccaatctc
     7441 atctaaaagt accaacgtat tcctttttat ctttttcaaa atagcagcga tgttttgcat
     7501 atgaccagaa aatgtactca acgcattttc taaattttgg tggtcaccga catcgacgaa
     7561 aatctcatca aagacagcaa gctctgtccc ttcttttgcc ggaacttgca taccgaacat
     7621 cgccatcaaa gtcaataaac cgactgtctt caacacgact gttttgcctc cggcatttgc
     7681 tccggtgata atcaagcctc gatagtcttt accgatttca acatccaatg gtaccgcatg
     7741 ctcaggtaaa aatggatgtc tgccttgctt gataatgata tgttccgatt tattgattct
     7801 aggtgtgctt ccattgattt ctcggctaaa ttttgcccga gcaaagatga tatctaacac
     7861 cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt tctgcgatag caccagtcaa
     7921 tgccgccagg acttgatatt cttcactgat ttcttcagct tttaacaact gatagtgttc
     7981 attcaattta gaaatcacag ttggttcaat aaaaaccgtc gttcttttat ttgattcatc
     8041 gataatcgtt ccatctattt tatttttata gctggctttg ataggaatcg tatagtattc
     8101 gcctttttga acaatcattg cttcttggat catttctttg tttttcggat gacgcaagaa
     8161 tttcagtaat tttgactgga tttctttttc gattagttgt aattgtttgc gtactttacg
     8221 caaattacgt gacgcgtcgt cactgacttt attatttttt attttctggt caatcaactc
     8281 ttctacattt atcaaatcag gaagatgttt gctataagca aacaacaagg gtgtctggta
     8341 ttgattctta tcaaaaaact ttgtgatcat ccgactgctt ctaagaaaat ccgcatattc
     8401 gatcaaatcg gttggctgca gaatcaaccc ttttttgact tgttctgtca gtgcatcgat
     8461 cctcggcaac cccataaatg gaacatgctg actgctttct aagatcaagc gtgcttctct
     8521 tgtttcttct tgacgatcca agacagtttg taggtttgtc gagaccgtca tatcgctgat
     8581 tcgttttttg ctatagtttc caattgctct tgcttggatt tcctttttga tccgatcaaa
     8641 ttgtaattga ctaatcattt cgttgtccat attctctcct tttcttgaag agatacctgt
     8701 cccaaaataa ataagggcct agagataaat aaaaggatag aacacagctt aaaaaagtca
     8761 tgtcctatcc ttattagaat acaaagatat tgttttcctg acaggtaccg tttcataagc
     8821 acaccaaata aactttgttt attggctatt cgacttactg attagatacc agctacactc
     8881 acaaaacata ccccattctt ctaggtctta tttattttcc acttaactat agcaagacac
     8941 attttggttg tcaaacttta gtatgtttta tagatttaca ccattaatga atagacacct
     9001 accgcgataa ttgctccgat cattggacct gcgattggta cccatgaata tgcccaatct
     9061 gaagttcctt tattagctat tggcaatacc gcatgtgcta atcgaggacc aaagtcccga
     9121 gcagggttga ttgcatatcc tgttggccct cctaatgaaa gaccgatggc taagatcaat
     9181 ccacctacag cgaatacgtt cgttccaggt gcaaattcgt tttgtccaaa agctagcaac
     9241 ccaagaacta atacaaatgt accaattaat tctgtgatca catttgctgg ataattccgt
     9301 atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt catctttcgt tgcattccaa
     9361 tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa aagctcctaa aacttgcgca
     9421 atgatgaaag gaacgacaag attccaacta attgcccctg tcatcgccat agctagagag
     9481 actgcaggat tcaaatgagc tgggcccatg aatccagaaa tatagacagc cattgttaca
     9541 gcgagtcccc aaccaaatgc gatgacaatc cagccagaag cctgtgcttt acttttgttt
     9601 aaattcacag ccgcacacac accatctcca agcaaaacta ggatcagtgt cccaataaac
     9661 tcccccatga tttgagtcat atcactgttc atttatttct ccccctttag ttcccgcaga
     9721 tctgattctg caatcaatgc ttctaaacgt ttagtctgtt gcgccttctc ctcttctgtc
     9781 cattcaaaat atccagcaat tgcatcgatc acaggttgct tgatctcatc aagctgatct
     9841 cgttcaaaca acagatgatt tgtacgacga atgagataat cgcctggggc caaaaccatt
     9901 tcttcttcta aaccataacg taaccgagct gactcagcga gactcaaacc aggataaggt
     9961 gccatttctt ttgctaattc aaagatacgt cgggcattag tcccgtaaaa atcagcgata
    10021 taagtagcat cttcttcctc taaaccggct gctactcctt cttttgctaa ttctgtcact
    10081 gtttcttcta atttagttgg atcgaagttt ccacctgaaa tctgatattt tttagagtcg
    10141 atttctttcg tctctattcc gtattcttct tttaataatt ggcgtatcaa tcgcaaagct
    10201 ccttctgcca tcttacggta atcggtgatt ttcccaccag ataaagtcag caaaccatct
    10261 ggttctcttt ctaaagaact acctctggaa atcgtagaag gtgctttttc atcacggctg
    10321 ttttccaaat ggttcaacac gtcttctact tcagcacgag aaactttgtt ttccttatat
    10381 tcacttactg tatcgacaac tttattgaag ctcttgtctg aaatcgatcc attatctcca
    10441 ccattgtaat cagaaccaga attaccaatc aacagtggac gaagtcctgc ccagcttgct
    10501 tcgatatcat tcaatgtgat atttgcttct ggatagcgat gattgatcac atccaataga
    10561 taatctacat cttcttgtgt gactttcgga tccgtaaaat ctccttggta atccgtatct
    10621 gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa aaaccatccg cccatcttgc
    10681 tttcctgtat cgaagtatgt gggttgcggt acaggcagtt ttttcgcatc tacaactaaa
    10741 tggatccctt tagttggacg cattttagga gagactggac gcgtaaaatt caagtttcgt
    10801 actttatcta cccaaggacc actcgtattg atcactaatt ttgctttgat ttcgatcact
    10861 tcatctgtca gcaggtcacg agctttgacg ccaacgattt gatcgccatc atataaaaag
    10921 ccaaccgctt tcattttact tactagataa gccccatctt cggcagcctt tttgatatta
    10981 tcaatcacta aacgggcatc attgttgcgg aaatctagat agacacccgc gccttttagc
    11041 ccttcttttt tcaaaaacgg ttctctttcc aatacttctt ctggtgtcag cgtataattc
    11101 tcatatttgg ttcctgtcac atttgccagt ttgtcataaa ggtccattgc tactttgacc
    11161 gagaacatat tgaaggttgt tgctccttcg ccctcataga ttggcaaaag cattggatct
    11221 ggttttggga tgtgtggggc aatactttgt acgactgcac gttcaccaac tgtgtcagct
    11281 actacttcca catcaaatgt cttcagataa cgaataccgc catgaacaag tttggtcgag
    11341 cgagaggaag tcccttctgc aaaatcctgc atttcgatca atcctgtttt gattcctgat
    11401 gctgctgact ggactgccac accggcacca gtgattccgc caccgatgat caacagatcc
    11461 aattcttctt gctgcatttt ttgaatgcta tcttgtcttg tcttgtttga aaacatatgg
    11521 atttcctcct tattcaaatg cttgggtagc ttttactgct tttttccaac cgttgtaaag
    11581 tttttctcgg cgctcttcct ccatttgaac ttcaaatagc ttgccagctt cataaaattc
    11641 gcggatctcg tctgtatctt tccaataacc gacagctaat cctgctaaaa atgctgcccc
    11701 tagcgccgtt gtttccaagt tttctgctcg ttggatcggt acattcaaga tatctgtttg
    11761 gaattgcatc agatattcat tgtttgctgc accgccatct actttcaata caggaatctt
    11821 gattccagtg tcatcttgca tcgtatcgat gatatctctc acttgataag caatcgactg
    11881 taaagtagct ttaatgatat cttcttttgt cgtaccacgt gtcaagccaa acatagcccc
    11941 tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg aatgcaggaa caacataaac
    12001 ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa tcgctagctt tttgcaacat
    12061 ttgaagtcca tctctcagcc attgaatcga agaaccagcc acaaaaatac ttccttctaa
    12121 agcatagtaa acctttccat ttatgccgta accaattgtc gtcaataaat tatttttgga
    12181 taattgcggt ttttcaccgg tattcatgac aataaatgaa cctgtaccat aagtattctt
    12241 gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg gcttgctggt ctcctgccat
    12301 ccctgaaatt ggtgcttcgc ttccataaaa atgataacct tgcgtataac cataaacttc
    12361 tgagttagaa gtgacttttg gtaacatcac tcgaggaata tttagtaaat ctaaaatctc
    12421 ctgatcccaa tctagctgat ggatattgaa caacatcgtc cgactggcat tcgagtaatc
    12481 agtaacatgt gcttgtccat cggtcagttt ccatactaac caagaatcga tcgttccaaa
    12541 catcaattcg cctttttcag ctcgttcttg cgcgccttct acatgatcaa gaatccagcg
    12601 tatcttcgtt gctgagaaat aggcatcgat cactaaccct gttttttcgt ggaaaaattc
    12661 ttgatagcct tcatttttta agttatctgc aattccagct gactgtctgg attgccaaac
    12721 aactgcatga tagattggac gtcctgtatc tttttcccat attactgttg tttcacgctg
    12781 gttcgttatg ccaatcccag caatctgatt tggtttgatc cctgattcga tgaacgctcc
    12841 ggcaataact gactgtacag aattccagat ttcatttgca tcatgttcca cccaactttc
    12901 ttgcgggaaa tattgcgtga actctttttg agaactgccg atgtgtcttc ctttcttgtc
    12961 aaagataatt gctcgtgagc tagttgtacc ttggtcaatc gccatgatat acgttgattc
    13021 tgtcattgtg attccctcca ttatatgcaa acgctttctt taactactct tatcatacta
    13081 ctgttttaaa aaaagaaact gtcaaaattt tattcttttt ttcttttttt gacagttttt
    13141 tattatgaag ctatttttta atcaattctt ttcttttttc agcgtctcta ttcttctttt
    13201 caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga taatattcac actgtttttt
    13261 gtttctctgg caaaatgtga tcaccaaatc atattcttta ccttcttcat aacttccaat
    13321 ctcaataccg ccgattcccc tcagttctct gatcagatat tgttgaaaag caatacgata
    13381 aatcggcaga ctttctaaat cgattccaat cactactggt tttgcaatat aaaaatcgat
    13441 catcaaaaga acattggcat actggatcat caggtaacta agctcactat cttcagctct
    13501 tttcctcggc aattgctgca ccgctgtttc ctgtaatttc gccagtaact gtgttaagga
    13561 atgcccgagc attttctgct gctgttccaa aagccgatca cgctcataga cttcgatctt
    13621 tccaacaaag aaaaaccatt cattgttgat ctgggaaatt tggtagccta ctgccttttc
    13681 ttctttgatt ttcaaacgac gaggacgata atgccataac atcgtttccc gaatgtaagt
    13741 atctaaaaca gcggttggta actttttgct ccgtgtcaaa tcatactgat aaaatatttc
    13801 ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac atcattgttt cgtatacatt
    13861 cagttctgct gctgttcgac taagatgcat cacgatgatt ggatcgattg cttgataaag
    13921 ccggtccgac tgatagagta atttcttttc tttcaaagtt gcgtacgtac ttttttcatt
    13981 caatagtctt tttttcgtaa tcccgagcca gcaagcaatc tgtgcttctg ctgatgccgt
    14041 aaacgttgtt tctaatacac ggtccaatcc tagaatgaac ggtctttttt ctggtgtatt
    14101 ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg aatagttcat aataaaaata
    14161 tcgtatctgt agctcttctc cgtataattg accgtttttg atttgtaatc caaattcctc
    14221 taagagctgg ttcaattctt tgatctttcg aaaaacagaa gactcactga tattaaacgc
    14281 tgtcgctaat cgaacgatgg atacttgctg atgttctaat aaatagacga gcatcttaaa
    14341 cttcaagctt tcctgcaata aaaacaatag aattttttcc aaacttacat cttcgtccaa
    14401 cacaagcagc gcctgatact catttcgctg tacttctact tttttttcca tcattcggcc
    14461 aatcaattca atatcttcca aatattgatc aaacgaggct tttgacagtt ggacatgctc
    14521 tctcaaacca ttgatagagg ctgttccccc ttgcagaaca aggtttcgca aaatcaggat
    14581 ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc ataggcagta ttcgccacct
    14641 ttgaccacac catatctggc acacggtatt ctggctggaa aaatagctca tgttcttcta
    14701 actgttcaaa cgtccatcct tcttctactg caattgtcag ttggtcaaga aggtggaaca
    14761 tctctttttt gctttggagc tgtgctccaa gaatcttttt ttcagaatca aatagaaatt
    14821 tgagtttaac tttgctttga tttattgctg taagcttgta gtctacgtat cctgtttcaa
    14881 ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc tacgcttcct aagaaatagc
    14941 caaaccactg attgccgact ggacgatagg ttccctgatc ctttgtctgt gagccactaa
    15001 ttgtcttgct gacatttcgt gctgtacgga cagcgttatt tactagagaa acatacattt
    15061 tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc aatcgcataa acgtcttttt
    15121 gagacgtttg caagtagtca tctacccaga tcgttccatc atcattcaat gttaattgtt
    15181 cttcccacat ctgattgtcg ggacgagaat gattagccag caaaacaaga tcatagttct
    15241 cctttctttt ttccgtctct acaatagcgg tttcattttc ggtcaaagac acaacttgtt
    15301 cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt tactaacggt tctgtcattt
    15361 caggatctag ataacgaaaa agaattgact ttcggctttc gtaaagatgg atttgttttt
    15421 ttcttgatgc caacccttca gctatttcga ttcctgcttg tcctgcacca ataattgcaa
    15481 ttttctttgc attcatgatc ttttgctgaa gagccgttac gttttgtatc tttttaactg
    15541 ggtgaataag cgttgattct gcagaaatat ttcgaaactt ttgatttgaa ccagtcgcta
    15601 gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa aacgactttt ttttctttca
    15661 atcctattac tgtcttttct gtgtaaacat ggattcgata gcggtcagtc agttcttctt
    15721 tcgtgatcca agaaagttgt ttttcactgg taacctttcc ttttaatatc aaattgaacc
    15781 cgctgggaat ccatcctaac ttcgattgct tttcaatcaa atatatttca cttgttggca
    15841 tcaattggcg aagataaata gccgcatgaa tccctgcaaa cgacccgccg acaattacaa
    15901 tgttcatctg cattcctctt tcttataagg tgttctactg ctaacttctc aatcagaaaa
    15961 cacagtattc ataactcgct ttcaacctta tgatacatag cgatgcagca gaatcaaccc
    16021 tctccatcat accgtgttct aggtctgctt ctcaataatg tactagtgga cctaattgaa
    16081 gagtatcatt tctgaagaga tgtttcaaaa aatgtactct tataaaagaa aaaaactcgc
    16141 cttttacgta cctctgtcca aataggaaac agaaaagaac gaaaagacga gttttagcgt
    16201 gtgtaatgta atagttagaa tagacacatc atttattttt aattctttaa tgcttcaaca
    16261 actgcttcgc ctaattgatg agcagattga ggattttgcc ctgtaagaag ccgcccatca
    16321 atttgaacca cttctgaaaa agctgctcca gactgatagt ttgctccttt ttctttcaaa
    16381 gcatcttctg ctaagaatgg tacttcattc gttgtaccat ttgcttcctc ttcttcatta
    16441 ctgaacccag tcactgtacg tccagctaaa atagattttc cagactcatc tgtaactggc
    16501 aacaatccta caacaccgtg acaaacagct gagaccagcc cttcatttcg atagatttgt
    16561 gaagcgactt tcccaagatc tattgcttct ggaaaatccc acattgttcc gtggccacca
    16621 gcataataaa ccgctgcata atcatttgca tccacatcat caggacgtaa ggtatttccc
    16681 aaggcccgat ttctgaaatc ttcatcttca taataggccc aatcgatttc atccatattc
    16741 aaaacacttt gcggttctaa aggtacatat ccgcctttag gactgacaaa atctgcatcg
    16801 atcccatttt ttactaatac atcataaaaa tgagtcagtt cacttaacca caaacctgta
    16861 ggacgctcga tcttttcgta cttttctaca tttgttaata cgatcaatac tttcattttc
    16921 ccatcctctt tctataaaaa ttgcgttatt gataacgtgc tttcaagaaa ttatataatg
    16981 ggcttggcaa ataaagctct ttatatgctt cttcaaaagg tttcgttaca aattcatcct
    17041 cacggttttc caataatttc tctgcccagt caggatcgac gatcatttgc tgtccaattg
    17101 ctgcaagttc tgcatatttc aatacctgct ctacatcttc gcgattgcgg atcccaccga
    17161 caccaactaa agggatcttt ccttgaatcg tgttatggac cacttcaaga atcgatttat
    17221 cgttgtattt atcagaacga gctattcgat catacatatt taatgaaaca tgcagataat
    17281 ctagttttga ctcacgtaat cgttcaagaa gccagatcgt gtcttcaaaa cggatacctg
    17341 gtgtttcata ttcttctggt gagaaacgat accctacgat gaatggtttt ttagcatatt
    17401 gttcaaccgc tgagaaaacc gttgttaata attcttcaat aaaacgataa cgattttcca
    17461 agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg agagaaaaat tgttggatca
    17521 aatacgtatt tgctccatgc aactcgattc catcaaatcc tgattcaatc gctctacgtg
    17581 tggctttgcc aaatgcttgg attgtttcaa cgatttcttc ttcactcatt gctcgtggtg
    17641 tttccgcatc aggacgtttt gctgcaactg cacttgcaga tacaggctgt tctccagata
    17701 aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg aataatcgct tttgcgcctt
    17761 ctccttggat tgctttcgct agctcactta atcgaggaat catttcatca tgagcgatgc
    17821 tgagttcacc tggccagcct tttccgatat cttggacatt tgcagcacca gtaataacag
    17881 caccaagacc tttagaacgt tgtttataat aagtgatctc atcttcggtg atcactccat
    17941 tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa tcggttcttc aattgaactc
    18001 ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt cattcctccc ataattcgct
    18061 ctgattaacc tatcgagaat catctatcat taaaccactc gattagttgt acatgcaact
    18121 aatttgctta aaaaatttta ttttttttgt aacttatctg ttgcatcatt cattgtctgg
    18181 gctaattctt tacttgcttc atttcccaaa actgctttgt atgactgatg aagttcttcc
    18241 cagctaccat cgacttcctt agcaaattgg cgtccttgtt ctgtaagata aactaatgac
    18301 aagcgacctt cgctaactgt tttgacgtat cctttatatt ctaatttttc aacaaaacga
    18361 gcaatcgttg atggggcgga atagagcatt tgtgcaatat ctttttgtgg tattccatct
    18421 ttttctttta atatcaataa aataaaacca taagttggtg ccagacctgt ccttctaaac
    18481 gcttcatcag ctaatttatt caacatgcga tctagctttt tcacgctaaa gaaaagacat
    18541 tcatctaacg ctttttcaat tggcttttcc atttcatccc ctccattggc gtacatatca
    18601 tagtatgatt tttatttttt gtcaaaaaac acggaataat gtccgcctcc tctgtctaga
    18661 tttgaaaaaa agaagtagcc atcttcaaag aatttcggct tgttttcgac aaaactcctt
    18721 ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta gttttgagta tgaagaaaaa
    18781 ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg aaaaactggc
    18841 ttttgaacac cgtttatatc agaggttgtg aaaaagccgt cctgcatcaa gtaataagaa
    18901 cagccaaaca aaaatagttc ctcatatttt gtcgtttttt gagctaatgc cgatggtcag
    18961 ctgattgtac accgtttatt gagaggtcgt gagcctgccg ttgaactccg agtcacaagg
    19021 agcaatttca aaaaacagct tctcatgttt tatgactatg gtgacttgtg atcgaggagt
    19081 tggcaggaga atactgttta ttcgtaaaga aggggctgtg acaagacttt tgtcacagtc
    19141 ccttatttcc ttttattata ggatttcgcc atttgattgg atgacttgtc tgtaccaatc
    19201 aaatgatttt ttctttaacc gtttctttgt gccattccct tcgtcatctt gatcaacata
    19261 gatatatcca taacgttttg acatttgtga agtagaagcg ctgacgatat caatgcagcc
    19321 ccacgacgta taaccaaata aatctactcc atctttgata gcttccttca tctgctcaat
    19381 atgctgcttc aaatagtcaa tgcggtaatc gtcttgaacg gttccatctt cttctacttt
    19441 gtctatagct ccgagtccgt tttcagctat gaacagtggt ttttgatacc tatcatataa
    19501 ttgattcaaa gcaattctca aacccttagg atcaatctgc cagccccatt cacttgatgg
    19561 aagatacgga tttttaattc ctccagttat taagtttcct tggacccctt cccaattttc
    19621 tggatgagca ctgactgcac tagacatgta gtagctaaaa gaaacaaaat caacagggta
    19681 ggctgctaag atttcttcat cacctacttc tatttttaag ttgacacctt tttcttcaaa
    19741 cattcgttgc gtatacgctg gataatatcc tcttgcttga acatcactaa agaacaaaga
    19801 agttctttgc tgctcaattg ttttttgaat atcatccgga tcacaagaat acggaacaag
    19861 aagctgatag ctgatcatac aaccgacttg tgaacctttg ataatttcat gacaagcttt
    19921 tgtagctaaa gcacttgcta taaactgatg gtgtgcacct tggtatttcg cttgttccaa
    19981 atggggatgg ttttcttcaa ttactcctac gctaacaaaa gggtgatgtt ttacacagtt
    20041 gatctcattg aaagtcatcc aatattttac tttattttta tagcgtttga atactgtttc
    20101 agcgaaacga acgtaaaact gaataagctt tcgattaggc catccaccat attctgtaac
    20161 caaatataac ggcatttcat aatgggaaag agaaacgact ggctcaatcc catattttag
    20221 acattcgtca aacacttgat cataaaaaaa taatcccgct tcattgggtg tttcttcatc
    20281 accatgagga aaaattcttg tccatgcgat agacatccga tagcatttaa aacccatttc
    20341 agcaaataaa cggatgtctt ctttaaagtg atgatagaaa tcatttccgt gtcttttagg
    20401 ataataatac ttgctgttgg gatcctgcgc cgcttttact tgctgatgcg tcatcatatg
    20461 ccattgatct gtccattttt ctttaggtaa atcgctgtcg aatgtataca catcagacac
    20521 agataatcct ttttcatcta catcccatgc accttcacat tgattggccg ctgtcgctcc
    20581 gccccataaa aatccttctg gaaatttttt catatcgatc cccctaaact tgctagtttt
    20641 cttgtttgat tatttttctt atgactagta atgatatttt tgaatctact actatttgta
    20701 acaataacca ttgtggtcgg atcatatccc tctttgatta attcttgcaa atctacttcc
    20761 atcaataaat cgcctttacg tacttttttt ccatcagata ctgcactttg aaagtatttg
    20821 ccatttaaat tcactgtatc aataccgata tggatcagta tctctgctcc ttcttcactg
    20881 actaccccaa ttgcatgttt cgttggaaaa actgttgaaa cttccccgtt tacagaactg
    20941 tagatttttc cgtccgttgg aatgatcgca aacccttctc ctaaagatcc actagcaaat
    21001 gttggatcgt ttacttcaga taaatcaatt atttcacctg ctgcaggagt acttaacact
    21061 atatcctttg aggattcttc ttcgatttca tccttgtagc cgaaaagaaa agttagcata
    21121 caggaactga tcatcgctaa gaaataacta gcaactagcc aaacaaatcc ttcaccaata
    21181 taaatcggga aagacaaaat acttcttgta gcaactgcca aagcaccggc tcctgcggca
    21241 ccagctactc caccaaatat agctccacat atacaagcaa tgtaaaatgg tcgtttatat
    21301 ttaagagtca caccataaac agcaggttca gtgattccaa ataatgctgt gataaatgca
    21361 gataaagata aactttttac tttcttattt ttcgttttca aaaatacccc aagtactgct
    21421 cctgcttgcg cagctacggc agtcatacat gctggaccta gcgtatcacg accgtagaaa
    21481 gaaagattgt tggtcattac tggtacaagt ccccaatgta acccaaaaat cactaacact
    21541 tgccaaaatc cgccgataac agcaccagat aaaacagggc taaagttata aacagctaaa
    21601 tatccagctg caatcagctg acttgcataa tctgcaattg gacctacaac taataaaata
    21661 attgggacca tcacgatgat acaaatgaat ggtgtcaata aatttcgaat actttcgtgc
    21721 aagcttttgc gaatcttagg ctcaatataa gaaagaaccc atacagctaa aattgctgga
    21781 ataaccgtac ttgtatacct tgctagaatc acgggcatcc ctagaaatcg caaggttatg
    21841 ctatcactat atgcactgac aatcgtagga taaacgagag ctgctgcaat aaccatagcg
    21901 atgaaacgat ctgtattgaa cttttttgca gcagtatatg ccaaaaatat aggcaagaaa
    21961 tagaaaaatg catcagctgc tgcattcaaa atgatataag ttcctgattc agtagttaat
    22021 aaattcaaac tcgtacataa ggcgagtact cctttgatca aaccacttcc tgccaagacg
    22081 cctaaagtcg gggcaaaaac agcggcgatc atgtctataa aagagtttaa aatgcctttt
    22141 ttctcagttc ctctctttcc actagatgca tcatcttcta gtttcatttg tttggataat
    22201 acgtcataca cttcatttac tgcatttcct actacgactt gaaacattcc tccagcttcg
    22261 actacagtga tcacaccttc tagttgctct accttctcct tatctgcctt cttattatct
    22321 tttagggtaa agcgtaacct tgttgcacaa tggtaaacat tgctgacatt ttcttcgcct
    22381 ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt ccatttttac ttcccccttg
    22441 ttaattaatg ccgtagcaat ccttcttaca agataagtat agttcttaga aaacggtttc
    22501 tccattcaat tagtacccct tctaatagcg aacgaaactg tacaatagtg tgtatggttt
    22561 acttagccga gcaaatcact cactgcttga aagaaaacaa gtatatcgtt tctcttatag
    22621 tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct gatgtataag tggtatctca
    22681 agctgatact ctgtgatttt tgtttctact tggcttttga taagacagca caacaaagtc
    22741 tcggcattta acggaagcat aactaggtca gaactaggag agattagttg ctgaatggaa
    22801 tctatctttt tatggatttt tatttgattt tcttctttgt agtaatgatc aagcagtttt
    22861 gttttctttt tttcttcaac tatggtcagc ttttcacgca aaatctgata aatctcccaa
    22921 gattcaaact catctagaac actaggtaga taaatcaaat catcccactc aaaaataagc
    22981 tcttgctcag tagtcaaaat tgcatcatat tccgttattt tgtttgattg atttttgaaa
    23041 agatacactg gttctatccg gcattctttg atccacatgt tcatactttg ttgaatctgt
    23101 ttttttagtg tattcaaaat actaattggc tggttactaa caatcagtat ctccccttgt
    23161 tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa aataagcgct ttcagctaaa
    23221 ttgggacgta attgaaaatg tattgggaag aaacgccgaa ttaaataaat ttctaaagga
    23281 taacgggaga tgatcttttg tacaaaatga ttcatggtat tatgtccggc atccatcctc
    23341 gttttcattt gtttgatatg ggttagcaaa aattctgttt catggaacaa taacgaaaca
    23401 ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc ttagattctt ctctatttct
    23461 ttgttttttt catcattaat catcaaataa tttaactcta atagtcgatt ttcaattgct
    23521 tgtttttctt gattggaaaa agaatagggt agttctgatg ataacttttt tgtgagggtc
    23581 tctaccatcg gaataaataa tgtatacctg tttgtttttt ctaaaagttc ttctcttcga
    23641 agaatggtca aaacgagata ccgagaaaat cgtatgaagt cctctcctga aacaatataa
    23701 ttatatgata taagaacctc ttttaatgtc ttttggacag tcggcatgat attggaaaaa
    23761 attgtactga tttgttgagg aaaccttgcg tgttggatca ccgtttctgt tccaacaaga
    23821 gataagaaaa ttctttttga attttcttca gcaatgattt ttagtccttt tgtcgacgaa
    23881 acttccagtt ctaatttagg attgcgctcg atccaacgca aaatcgtttt tatttgtaat
    23941 gatacggctg tttttgaaag aaagaaaatg tctgctatct tttgcatact gacataccct
    24001 ttttgaaaca ataagtacgt cacaatagtt gaagctcgga aatccatgct ggaatagatt
    24061 ttatgtttcg tcacctcttg tatgatttgc tgtttagttt cagattcaat tgattctaaa
    24121 aaataacctg aacgtttatt tgactggata atgattttct tctcgtagat agcaagcata
    24181 tttttgattt cagtttggat cgttttcttg ttggtaccta aagcttcagc taattcttcg
    24241 cttgttatcc aatgattctt atttcttaat actacaagaa tttggagttg tcgtttattt
    24301 aagtccattt gcttacacct cctctttgtt ataaacatac aggaaaaacc tctcaaatga
    24361 caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa gatctatatt caaggttttt
    24421 accattagac tggataattt gtttataata ttcaaaacta tcttttttca atctcctaaa
    24481 gcttccgctc ccatcatttt gtctatccac ataaataaag ccatatcgct tcctcatttc
    24541 ccctgttcct gccgatacga tatcgattgg tccccaccat aagtaaccaa tgatttggca
    24601 accatctttt attgcctcag caatttcctg aagatgtatt tttagatact cacaacgttt
    24661 tggatcgtgg attcgattca attcatctgg tgcttcatct agtccaatgc cattttcaac
    24721 tatgaataat ggtaattcat aacggtctgt caattcattc atgacataac gaaaccctag
    24781 aggatcaatt ggccaactcc aaggttcggg agatttttcc ttcaaaaagg gattatcgaa
    24841 accatttgct ccgccagtat ctacgcgcat ttctgcttct tttttataga cagcgctacg
    24901 ataataacta aatgcaatat aatctgccgt gttagcttgc aagcactgtt cgtctccatc
    24961 taacattact ggtgcacagt cttcttcctt ccatgtcctg taaatataag ctggataatg
    25021 ccctcggcac atgacatcgg agaaaaacca tgaattgcgt ttaaattgca gtgaaccaaa
    25081 tacattctct ggatcacagt catatgcata agtcgctaat gcagaaaagc tacacataat
    25141 acccatctta gcatttggat cgattttctt taataattga gtagcacggg cgttcgctag
    25201 aaacatatga tgacaggctt gataaatcac tttttttgtt agtgtagcaa tgggttcaag
    25261 tttatctcta gcagggaatg taaccaatcc agcagctgca taaggcatac gaaaagcatt
    25321 attgatctca ttaaaggtca tccaatattt cactttccct tggtagcgtt caaagacggt
    25381 ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt tgccaccccc catactctcc
    25441 taccaatgct aagggggttt cataatgact aagagtaatt actggctcga tatcatatcg
    25501 atttaatgta ttgattagtc tgtcataaaa tacgagacca gcctcatttg gcttctgttc
    25561 gtctcctctt ggaaatattc gtgcccatga gatgcttgtt cgaaaagctt ttaaacctaa
    25621 ctccttcagt aaacgaatat cctcttcaaa tcgatgataa aaatcaatcg cttcatgaga
    25681 aagataatgg agtgattcgt ctaaagcaat ctcccaaatc tttttttctt cattccactg
    25741 aatactagga tgctggttca tgatcccgat aagcgtatct gtcacattag gtaatttgcc
    25801 gtcttccaac catgcacctt cacattgatt agcagctact gctccgcccc ataaaaaatt
    25861 ttctggaaat atttcctgat ccataactat ctctcctcgc tatcaacaat ttcttacttg
    25921 aagtatagct cgattcatca aaacaagata ttctaactat ttcggcagaa gagaagaaaa
    25981 aaattgaatg ctactcttca tttttaatat ttatatataa caaatacata agacgatccc
    26041 cgaaagaaaa cactcgttaa ccatctaagc aaacgattta ttttacttcc aacacaaatg
    26101 cttcataagg tcgtaaatca actttgccgt ctggtaactg tttcagagga taatttgaca
    26161 caataaccct taaagcttca cgtggaatag tgaacgtctg cttttcactg gacatattac
    26221 aaataacgag ccattgctgg tcttcatata tgcgagaata ggcaaagaca tgctcaggcg
    26281 tcttttccac taattcatag tctccccaga cgatgatgtc atgttttcta cgcaaatcga
    26341 tcaatttctt gtacatataa aaaacagaat cttgatctgc taacgcttct tctgcattga
    26401 tttctttata atttggattg actcgaagcc atggaattcc agtagtaaac ccagcatgtt
    26461 cacgggcgtt ccattgcata ggcgtcctag cattgtctct tcctttcgcc ttgattgatt
    26521 ccaagatttc ctcttttgta aatccagaag aaatcctttc gttatacata ttgattgtct
    26581 caatatcttt tgcttccgcg atatcatcga ttgggcaatt cgtcatgcct aactcttctc
    26641 cttgatagat ataaggggtg cctttcatca tatggagcaa gatagcaaac atcttggcac
    26701 tttcttttcg atacacatcg tcatttcccc aacgagaaac aattcgcgga agatcgtgat
    26761 tattccaaaa taaactattc catccttcat cacctaaaga tgtttgccat ttggataata
    26821 cttttttcag ttcagagata ctcaagggtt tcaaatccca tttgtctttg ccttcttgct
    26881 gatctaatcc tatgtgttcg aattggaaga ccatggataa ttcatttctt ttaggattgg
    26941 aataaagttt agcgatttcg gtagttgctc cccatgtttc gccaacagtc atcagatttt
    27001 tgtcaccaaa agttgcttga ttcatttcct gaagatactc atgcagttta ggtccattac
    27061 ttgtgatttc tttatccggt tctttgccaa tcatatcgat cacgtccata cgaaagcccc
    27121 caataccttt atcgatccag aaattcatca tttgataaat ctcttggcga acctgtttat
    27181 ttttccaatt caaatcaggt tgacgcttgc tatacaagtg cagaaaatat tgccccgttt
    27241 tttcatccaa ctgccaagca gaaccactga atattgagcg caatctatta ggctcttttc
    27301 cattgaccgg atcacgccaa atataatagt cccgataagg attttcttta tttttacggg
    27361 cctcaatgaa ccacggatgt tcatcagaag tatgattcac caccagatcc atgatgatac
    27421 ggatatttct tttctttgct tcttcaatca gttcttccat ttcttccatc gtaccgaatt
    27481 ctgccatgat tgcttgataa ttactgatat catacccatt gtcatcattc ggagactcgt
    27541 atactggaga aagccaaatt gcatcaatac ctaaatacgc cagataatcc aacctggaaa
    27601 taatcccccg caaatctcca ataccatctt gatttgaatc ttgaaaactt cttggataga
    27661 tttgataaac aactgaatgg tgccaccatt tttgttccat gattttcctc ctataaatgg
    27721 atgtttctat tttttgctta atcatttatt ttagtctctc tcactaaaaa ttgtaacgct
    27781 ttcaaatagc tattgcacct tgttatcggt aacagaataa aacaaaaaaa cagctgagga
    27841 aattccctca actgtttttt gaaaggattg atttatgcag ggaagaatgc tgatttcaca
    27901 acttctttat aaacggtgtt cactcagctg tcccttggta ttagctcaaa aaacggcaaa
    27961 atataagaaa ctattttttc gtttttttct ctaatactca ggccagaacg ctgatttcac
    28021 aacctcttta tttcacattc aatatccaca tgaatcccac aaatacaaag aacagaccat
    28081 acatgattgg agatacttct tttcctcttt tagctgctaa catggtaatt ggatagaaga
    28141 taaaacctaa tgcaatccca tctgaaatac tataagtcaa aggcatgccg atcaagatca
    28201 agaatgaagg gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca
    28261 tcaatactcc taccataatc aatgctggtg cagttacttg tgaagtaaca actgatagta
    28321 atggagaaaa gaacaagcct aacaagaaga aaatccctgt agtaatagct gtgatacctg
    28381 aacgtcctcc aacggcgata ccagctgatg attctacaaa tgcaccaaca ggagaagtac
    28441 caaataagga acccgcaagc atagctgttg aatcagaagc taaagctttc cctacacgag
    28501 gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa tccaactaaa gttcctgctg
    28561 tatcaaagaa agtcacaagc aagaatgtca gcactacgac ccacatttga agagaattga
    28621 tgtcccctac atgttttaat gcaactaaaa atgttggttt caaacttggt gctgcggcaa
    28681 caatttcaga aggcattgga atcaaacctg tgaccagacc taaaatcgtt gtagctgcca
    28741 tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa gatagcagtt acgactaatc
    28801 caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc taatcctact aatgtagatt
    28861 cattcgaaac gatgatccca ccttcgctca gaccaaggaa agcgataaat agaccgatac
    28921 cgccagaaat cgcatatttc aaatctgctg ggatcgcatc aatgatcatt tcccgcagct
    28981 tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa tacgccggct aatgctgttt
    29041 cccatggaat ccccattcct aaacaaacag aataagcaaa aaaagcattg atcccaagag
    29101 caggtgccgt tccaattgga taacgggcta atacccccat taaaacacag ccgatcgcac
    29161 tggctaatgc agtcgcagta aacactgctc cttcatccat tccagaagca cctaatacag
    29221 ttggatttac aaataagata taagccattg agataaatgt tgtaaatcct gccagtacct
    29281 cacgttttac cgtggtgttc aatttttcta tttcgaagta ggaaagtatt ttttccttca
    29341 tcttccatcc ccctaaatcc gaacattata aataaagtcc gttaaaattt cgatttatta
    29401 ataaaacaac gtgttttatc atatactaaa acaaaacata agtaaacaaa aaattcggat
    29461 tttaagcaaa atccgaacgt tccatctgat taaatccctt tatctcaaag ttcatgaaca
    29521 ttcactttgg attacaagaa tttctttatt gtgttaataa agtttcttca ttctagtctc
    29581 aaaaatcaat agtgtgattt gattttacca tttcgtttct gttcttcttt gatgaattct
    29641 tttgtatcga caggattcag attatttagt ccgccaaaac ctgtatcgac catttttccc
    29701 cagccttttg tcttcttttt tcctgatttg agattctttg ctcgaagctt ctcactatgt
    29761 tttgctcgcc gatacttcaa ataaccttct aatcttccca ttagatccac tgtttttcct
    29821 cctttttatt caatcagacg actcagcttt tcctataaca aaataattgc cttcagggtc
    29881 agaaaaattg aaagtcaaga tgccattgtt atctgtgatt tctcctggtt cttccatgtt
    29941 ctcgtagata gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg gtcctaatac
    30001 ctctggtgaa aacttctgca caaatacttt tgggaatacg cctaattcta attcttttga
    30061 gatgctgagt acaatactct taaattcttc tggcaattcg attgtttcag aaatttctgc
    30121 accaattttt tctacaaaga atcggcaaat catctctacg tcattgacat aaagcatcac
    30181 acgtgttttt tgtatcatca tttgaccctt ctttctattt gattttcttt cctgtctata
    30241 aactgattcc attatatcac gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa
    30301 ataaagcaat agtttctagg attgctttcc tccacaaact ttttttacaa aaaaaaaacg
    30361 gcaatgaatg ccgctcttct tatttcgatt cttttataat tctgcttctg attcatcttt
    30421 ggttagtgtg tgtctaacac tagttacgcc aaatgtttga tcgatttttt ccttcaacgc
    30481 ttcgattgcc tgtgcgccac gtgccgcttg ttcagcattg atggattcaa tgaccagcac
    30541 cgctgcttgt gtttcttccg tcagcatcgt tctttgggct tcacgccagt tgagacagcg
    30601 acagatcgct ccttgttcat catagtagat gatttcttcc ggaagtgccg gagcatcgga
    30661 atctgcacct aaaggcagga atgattcgcc accttgggct tttcctaaat gcagatttcc
    30721 tgcgatcttt tcgatatctt ctccgccgca aggtacgcca aaagacaagg aaatactatt
    30781 atagatatct acaagcggat tgattgggaa aaattcccgt tcttgatttg cacgtttcag
    30841 taaagcttca atagaagaac gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg
    30901 ccactgagca atgacttcgt tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc
    30961 ttttgctgcc tcattcaata attgatgata cgtttcttca ctttcttttg ttacgtggtt
    31021 atcgatacca tttacggata agatttcgat ggttgcttca ggaaatactt cccagaaatt
    31081 ggtatccact attacttttt tcatctgttc acacctcttg attagaataa ttgcttgatg
    31141 aatagatagt ctgtttgggg atcatctccc atataaaaaa catgttcgct gtatttgata
    31201 aatccttttt tacgataaaa agcgagcgca acatcgttgt gttcccaaac accgagccaa
    31261 atggctgttt tttgcttttc ttttgctttt tcaatagctg tatctagtaa aaatgtaccg
    31321 ataccttgat gtttaaattt ttttcgtaca tagattcgct cgatttctaa aaaatgatca
    31381 gccatttcct cagattgact cgttcctgta tttagtttca ggtaacccgc aggctcttga
    31441 tcgatactca ctaggaaaaa atcggtttct ttatctttta gctcttctgt aagtttttct
    31501 agtgcataag cttgtgtcaa gtaatcagct aaatgctccg gtgaattatc ttttccaaat
    31561 gtatctgtaa atgtttcgat actaatttgt tgtagtaagt ctacatctga aggcgtcatt
    31621 tttcgaattt cgtatttatt cacggcttct tcctttcaat agatcctttt attgcctttt
    31681 ttgacaaaat tccaatcttc gctaacattc ttgcatactt tatttaaaag atattccaat
    31741 tgttttgctt cttcgtcaga aagaccattt aatgcgactg cattggagta gttgttttct
    31801 cgaatgatga aaggatagat ttctgctcct tttttagtcg gatagagttt cttgattttt
    31861 ttattttctt tatcttccaa acgttcgatc atcccattgg attctaattt cttgattgct
    31921 cgggcagtcg ttgtacgatc tactttgatc atttctgcta gtttttcttg gatgattcca
    31981 ggattttcgc aaatacgtac taaataaaga tactgtcctc ttgttaaatc gacttctttg
    32041 aattcgatat tgctaataga atctagcgct cgggcaatca ttccgatgtc tcgaaggatt
    32101 tcagccattt catcccacct ttattttgtt tgtaagacta gttcacctct ttttgttgca
    32161 aatgcaacta ttttcaataa aaaaatcaag ttctttttta ataactagta aagtatattc
    32221 ttttcttctt ttacgaaaaa gctcaatcac aatagatctg gtttttgttt tctaaaataa
    32281 gtaactgttt cttcgttgtt cgcttttctg atagctttgg caactcttct atactaaaaa
    32341 atcccatgtt ggaagtttca ttattctcaa taaacttggc ctcttcaccg tggatcgcac
    32401 aagcaaaaac aagtttataa tactgaaaaa gctgtggaat atctttttgt ttattcgtat
    32461 caaaaactgc tcgcaactga gtagtttcta ctgttaatcc tgtttcttca tagacttctt
    32521 tacgcacatt ttcttcagga gacaatccta tttcagcaaa tccaccagga agtgaccatt
    32581 ctttcgtccg tttatcttcg attagtagga ccttgccatc ttttttgata taggcacgaa
    32641 catctacttt tggtgttgga tgtccttctc caatcgttgt gagcgctttt atttcttcga
    32701 aagtttcttt tcctatttca ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac
    32761 gctctttatc aaatacatct tttccataat atagaccagc ttgagaaagt gcgagtagtc
    32821 ttttgtattg agccaacttc ttgttcattc tcttcccttc ttttttagca aatgtatgac
    32881 tatgtaatca agtatagaag actggtttaa ttacggcaaa taaatataat gaaatctgca
    32941 ataaataaaa gaatgttaaa aaactcgaga acaaaagaca ttcgacttct ctggggctgt
    33001 gaataaatgg cgttcaaaag cttgcatctg cggtaataag ccccaccaaa cgaaaaatat
    33061 gacgagctat tcttgtctgg ctgttcttat taattgaggc aggacagctt tttcacaacc
    33121 tccccactaa acggtgttct cctaccaact cctcggtcac aagtcacaat attcatcaaa
    33181 catgagaagc tgtttttttg aaattgctcc ttgtgactcg gagctgaacg acaggctcac
    33241 gacctctcgg taaacagcgt tcaaaagcca atttttcgat attaagccat ttctttgtta
    33301 cttattgtca ttgatttctt tttctaattt atccaatagt ggagtgtaat ccacttgagt
    33361 atagtctgtt acatcaaatt catatagtgt tcctaactgg aatttgtcat attttcggtc
    33421 ggcgatgatc tttttgaatg cttctttcgt aatatgattg gtccctttcg ttcgatcttc
    33481 caatacgtca ccatagtgat aatgatccat gatatagctc aaatgacgat ctgattcacg
    33541 atcacgtttg taccgacgtt gccataacac ttcaaagtct gctgttaggc gaatcgtgat
    33601 cacctgatat ccgtatctat ctgctaattc ttggagtcgt gctttttgct tatcactaaa
    33661 aggatactcg gacacaatca ctttctttcc tgcttccata tacaaatcaa gtgctttgta
    33721 atagaaagga taaactttcg tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc
    33781 gatggattct gcatagtatt ccttgatttc atcgagcgtg atggcataca tatctggaaa
    33841 tcgttcccgt agtttattca taagatatgt ttttcctgta ccaggactac cagccagaag
    33901 aatgaaatac tttttcatct catttatttc ctaagttaat tggactttcc aagttcataa
    33961 acagtccaat cttatgcttg acaatttctt ttactgcttc atttgctgga atcacattat
    34021 ggcgaagaga cttgttgatt tctgtttcac taaaggtttc tttagctact ttcgtccagt
    34081 tgactaaaag ctccgttcca acattgaatt tgcggatccc gttattgatc aattcaggat
    34141 aatcttcttc tttcacacct gttcctccat ggataactag ggggacatca actactgcat
    34201 cgatttcttt cagcaaggga atgtttactt ctgtttttga tttgaattgt ccatgattcg
    34261 taccgatagc aatagctaat gcatccacgc ctgtagcttc tacaaatttc actgcgtctt
    34321 ccggtcttgt atataccttg tcattttcag cgacatggat tccttcttct gtaccgccga
    34381 ttgttccaag ttctccttct acggaaacac cgtgcgcatg ggcatattct actactgctt
    34441 ttgtcttcag gatattttct ttgaaaggca gatgggaccc atcatacatg acagaagtat
    34501 atccgctgtc gattgcttct ttgatatcat caaagtctcg ggcgtggtct aagtgcaaga
    34561 ctgcatcaac catttcttct tctgcaatag ctgtcattac attgactaac acatgcatgc
    34621 cgatatattt agcggtatcg acacttgttt gaataatgat cggcgaaccc atttcttttg
    34681 ctgcgcctag catagcagga agcatttcta ggttatgcgt attaaaagct ccaactgtaa
    34741 aattcaactc ttctgctgtt tttgttactt ctttcaatgt tgtatacatc ttttattgcc
    34801 accttatctt tttatttgtt tcctttgatc gttacttgtt ttgcaatttg tcgcatctca
    34861 tccattttgc ccatgtagta atcgatccct tgatcatcac cagcacgagc agcatctgca
    34921 cgcttctggt tatagaggct catcagtttt ttatacccat ctccgatcgt ctgtttattt
    34981 tccaaactct tttctaaata ttggatggct tgatcgttca catacaattc agaaaattta
    35041 agtccaactt gttcataaag tttcgctaat ttatgcttat cttgggtagt ggaaatctct
    35101 tgtttcaatt gtgtaatctc tgattgaatc ttttcggtcg cttctggcga tagggctttt
    35161 tcttcttttg ccagaacttt tttcggttct ttttcttttt tcttaaaaaa tccaaacatc
    35221 attttcccta ctttcaatta aaatgctttc aaaactgggc ttaagatcca ggcatataag
    35281 agtgctgcgg ctactgtatc tacatcggtt gctgtagcat tgttgaatcc catcgaatta
    35341 aagatcgtta ctagcaaagc gggcaataat gtaataaaga aaccatgagc aataccgcca
    35401 atgatcgctc ctcggcgtcc gccaactgcg ttgccaaata tccctgctgt ccctccggca
    35461 aagaaattgg ttagcatacc aggtaaaatc atcgctaagc cgaacatggg caaagtaaac
    35521 atggcaataa tcgtaccaat cgttgtcgta atgaaaccta aaatgacagc atttgggcta
    35581 taaggaaaga aaactggaca atctaaggca gggatcgcat caggaactaa tttcatagct
    35641 atccctcgaa atgccggcac gatttcacct aacagtagac gtacaccagc tagcaataca
    35701 tacacaccca ctacgaattg gatggcttgt aaaaaggcaa acatgatata gttttgatct
    35761 cctgataatt cagaagcgaa tggttcgcct gcaaataaaa cagtgatgat atatagcggg
    35821 accatcacga ccatgacaga caaataagta tcttgcagaa attcgaaatg tgtcggcaac
    35881 ttgatatctt cgatcgactt tttattttcg cctttctcac caaaaagctt agcaacaccg
    35941 gcttcaaata agtacccgat cgtacaaaaa tgacctaaag caatatcatt tgatccagtg
    36001 attttacgaa tgatcggttg cgctactgcc ggcatagcaa tggcgaaaca agctccgata
    36061 aaaccaccga ccaagatcaa tacgatccct cgcagaccgg caaaatagcc aaagactgtc
    36121 gtcatcgttg ccatccataa aattgcttga ccagttaaaa aaatgtactt ccattttgtg
    36181 aaacgagcca aaataatatt gaatacaaaa atggctaaga acgtcagtgc aatatctcta
    36241 cctaaaccca aatcattcat tgcttgacca ttgatagatt caatagaagg gatgatccct
    36301 tgcatatgaa atccttcagt aaagattttc ccgaaataag ttaaacttcc aacgatgatg
    36361 ctcgaaccgg cactcaacac ttgaaatcct aggagtgtct ttaacgtgcc agagatgact
    36421 tgtcctgctg atttcttctg cagtagtaga cccaacatag caatcaatgc aatggtgatc
    36481 gatgcctgag tcaaaatatt ttcgatgata aaattgataa cgcccatgtt tttaccctct
    36541 tttctttttg tatttagata actcccttat cttgcagaac tggggtgatt ttttcttcaa
    36601 tttcattttt tgaaacaata tttttcaaat agatcatcgc cgttttagga tcaactgcga
    36661 acttttcgaa ctgcgaacga aaattttctg ctgtgatgat cacatctggt ttcatggaaa
    36721 ctgcggaaga aatatcacaa tggtccagtt tggcttctac tcctaatttg ttcaaaacat
    36781 cctcagcaga catctgtgct gcaaaactac ttcctaaacc tgcaccgcat acgaataaaa
    36841 tgttcaattt gttcatcttt gttctccccc gtatcattta ttatttctca actactgaaa
    36901 aaagcagttg tttgaaatct tcgatatttg tgcattcaat caatcgatcc aacttctctt
    36961 cgttgttgat caattcaatt agttctttca tgatgttcag atgcgagtag gagtctactg
    37021 ctgccaaaca aaaaataatc tttactggat ccatttcagg attaccgaaa tcgattggtt
    37081 gctcaatcgt agctacgctg actcctaatt tattgacccc atcttctggt cgggcatggg
    37141 ctagtgccat gtgtttgcca atcacgatat acggtccgta atcattcact gcacgaatca
    37201 tcgcttcgac atagcgctct tcgacaatgt tttctttaac caatggtcta gcagctagtt
    37261 caatcgattc ctcccaagta gtggcttttt ctttgatcat gatattgctg tccgtcaaaa
    37321 tatcttttaa cataggctgg atctcccttt tgttgatttc taaattgttt gtttcgaata
    37381 atttttctaa cttgccatag atgattcgat ctactcggcc accactttct ttcatgacat
    37441 ctattatttg agtgaataac tccgtcgatt catgactgtt aggctctaac cgctgtagtt
    37501 cttcatttct tttcaaaaag tcttctataa ccaagatatt cgtctcagtt aaaatcggat
    37561 cgacaactaa aagcggctta tttcgataat tcaattgaaa ggtgctgaaa accagattga
    37621 catccaattt atctactaat tcgatttctt ttgaacttaa aactgcagta atctcgatct
    37681 gaggaaactt ttctttcaga ttctctgcta acagatttcc agtagccatc ccatggttac
    37741 agacaacgac tgatttataa acataattca agtctcgctt gattacactg gcgatcgtcg
    37801 aaaagtggat gacaagaaaa gctatctcgt cctccagtat cttgccacca attacttctt
    37861 cgatcgtagg aacaaaatga gtgactgcgc ggtagattgg gaaataattt gttcggatgt
    37921 tttctttcaa tggattcatg acttggatat gattcttgat tcttacaatc agtgcagcca
    37981 tgtgcttata gagattttcg aataaagctt cttcctttag atggaaaggt acgtgtgtct
    38041 cgttctctac aaattggatc aattggatcg ttagtaattg agcttgtacc cactcgattg
    38101 aattggagat atctctagca ttgaacgtct caatagtgaa acgaagataa tttctttcta
    38161 cttgcgggac attgtccaat tgaaattcct cgatcatgtg gtcaataaat gcagaaaagc
    38221 tactgtcttt tatttctgta tttttccaat taatggcaga gataaattca tgtttcctga
    38281 ttcgctggat ccagatcaat gtaaaaagca ggatttgttt tttataaata tcatcttctt
    38341 gtttcaacaa cttattggaa tagatctcct gtattttttt gatttccacc aaaggaaaat
    38401 aatggagaaa gattttttga gccggtgtta attgtgcatc ttgattcata aagaagtcgg
    38461 tcattcccag attctgattg ataaaatcat agatcatcgt acggatagat cgttctgaac
    38521 ctttataaac cagtccttgc tttccataac tgacgatctc tatcccataa ctgaccagtg
    38581 ttgctcgtaa ccgtctcata tcttcatcta atgtactttt agagatttga aattcttctt
    38641 cttttttgtt tagatggatc gggattcggc tgtaagcaac ggctaaaact agatcaaaaa
    38701 tacgttcgcg ccgattcaaa aagccgtctt tcacatcttt tatttcagtt tctaatcgag
    38761 cttgcttttc cttttccagc ttcaacaaaa aacctttgtt gcgaattgta cggatctccg
    38821 gaagcttttt actgctcaac cagatattcg cttcaagaac ttcctgacgg atcgtccgct
    38881 gactgacatt gaactcactt gctagatgtt ttgcttttac tggatattgg gctgtcatga
    38941 ttctgtgaag tatttcaatt gttcttgttt tcatagaaat gatctccttc tgatttcaag
    39001 tatatggttc cgcttacata ataaaaagta gatatttctg ccacttgaag tggaaagaaa
    39061 gtaagcaaag caaacaaaaa ataagccata tgctttttca tctcctcaac gaaaaagaca
    39121 tacgcctatt attacaaaaa ttttgcccat ctatacaaaa aagaggttgt gaaaaagctg
    39181 tcctgcatca agtaataaga acaactaaac taaaatagtg cttcctattt ttcgtttggt
    39241 tgttcttatt atcgcagatg caagctttta aacaccattt agtggagagg ttgtgacaga
    39301 gatgttcagc cttgagcgat aagaagcgat tttgaaaaat cgcttcccat attttacaaa
    39361 atcgtgcctt attgccgata ggctatctct ggcacaccgt ttattcagaa aaaggagctg
    39421 cgacaaggct tttgtcacaa ctccgttcat aaatatttcc tgtcaatcca gcataatcgg
    39481 ctgaataaaa tcggcttgcc atcttttttc tttctcagaa aaatcaaaat accatatctg
    39541 agaaaaatac tgtcgttgtg tcttacttcc tgagatcaat cgcccatctt ttcgatcgac
    39601 tgaataatca cagcatctga aatcaattcg gatggcaaaa ctattttcgt ggatctgtcg
    39661 ataatttcct acttcctgaa tagttatttt tttagtatga tttcttacac cgtctgcttt
    39721 gtttttttgc agaactttct gatgttcggc aaacagcttg tctgtataac agcttctggc
    39781 tttttcagga gtttcttctt cccaagcgga ttggatcttc atgaatatct tttggatttc
    39841 cagaaactgt gctttctttt ctttagccgt tcctgacatg cgggacaata tctccgcttt
    39901 cagccttttc ttttgtcttt tattcttgac gatagaaaaa gcagaaaccc ctcctactga
    39961 caaaacagct aatgcaagaa atcccgtttg ctcataacta gaaggacctg tacctggacc
    40021 ataataccca taagaatctc tgccgtaaga acgactgcca aaataactgc ccccatatga
    40081 acgaccacca aatgaacctc tgcccgaact tcgaccgcta gatgtacctc caccatgacc
    40141 acccgcgaca gcgaatcctt gggtcggcag agcaaacaaa cataggccca ctaacaaaat
    40201 gattgctaaa taattgattt ttttcattgt cttcctcgtt atcttttatt acaaaaaact
    40261 atttttatga tagccttgct aaaaggaaga gtcaataacc agatgcataa actgtttttt
    40321 tagcaatttt gtcttattta gtcattgagt aacatcggac aaatgtatgt gtcggcagtc
    40381 cataattcct ctatcttcag catgaacaag cttgcagaca agagatctgc gcttcctcca
    40441 ggagagattt ggttttcctg acaaaagtgc aacagtgctg taaatggacg atttggtaaa
    40501 gcactttgac tagcagcatg agcaagtttt tgcatttcca ctaacgtttt ctgatcactg
    40561 cgatgcaata cgcatgtgtc atctagcgaa ctcatcaaaa ctaacaacat atgcatttgt
    40621 ttctgtacat atgaatcatt atctagctcc ttagctgctt gaattgcaag cctgatatgc
    40681 ggatacccta atatcgcttc ttcatatgca ccatttacgc catatttttg cttcacccta
    40741 tgaccatgag tggttttttt atgacgatat tttgtatcag gaaaagcagc aagcttgctg
    40801 acatcatcaa gtattttcga taaagaaaaa ggctgttcca tactgatttt ttgtgcaatg
    40861 acactgatca atagccccat tgcccaaatc gctcctttat gggtattgat gccatttgta
    40921 gcaatcagca ttgccttttc cgcttttcgc ccaatctcag caattctttc tcttaactcc
    40981 tgatcaatag catgatgcca tgctatttga gccatttctt caaaaaaagg agttagcgtc
    41041 ttagcagact taagaaataa agcatagtcc atatcggaat gtgcacccgc atccagtgca
    41101 tccactaatc caggttttgg acttaatgtt acttctgcga tcaatgcttc ctctaccaac
    41161 gcagcgagtt gctgagtcac tgcttttttt cgttcgtcat ttatcatgac actcactcct
    41221 tcttcctact attacttgct gaaacctttt ttgatagcta cccaaatgat atgaagtaac
    41281 cagcttaaag caaatgcaca acttgccgtc attagataag ctacaagaat catgactcct
    41341 gaaggtaact gcagccaact atctttaaac aataataaca gtacttggaa aacaaataca
    41401 ttggctaaat acgtacgata agcataaaca gacaaccatt tgaacaatgg caattttggc
    41461 gaatcgttta caatcatttt ggaagcgcct atgaagacag caaagatgat gactaaactg
    41521 tataaacttt ggatcgtatt caagtaaggt gcattcgcaa agctcaagtc tcctggatgt
    41581 gcgagcaatg aacggacaga aaagaacgcg ataactaagg caacaggtaa gaaaagataa
    41641 cgtactctgt ccaaatattg atagactttt tcgtgataaa caaatgcggc taccccataa
    41701 atcatgaaac tagcaacaaa ccgatctaat aagtaccagc tgtcatgata aggcccagtc
    41761 aatacttgtg tttgatagaa cagataccat gcgacatata aaatcgttga accaatcaat
    41821 actggccata catatttttt attagcaaat actttgtaac cgagccaaac aaaaaacggc
    41881 atgaacaatt ggatctgcag catcataacg gtataccata aatgaggcgc tccatcacct
    41941 gtgacatatt tcagtaaaaa gcttgcagta tccgtatacg gagtttgttg ttggactgct
    42001 ggaaatagcc aaagataagc cgtcgtccat agaagatacg gaacgactag ttcaaaaaac
    42061 ttttctttca aaaattccca ataagacaaa tgctgacttg tttttaccat attataaaca
    42121 attgcaaaaa taaacattgg tgctgtgtat ttaacgatga cgtaaaaggt accaagaaaa
    42181 gctgtatttg ttgcgctgtg gctgtttgcc aaagcaaagc tcaatatcgt ttgcatcata
    42241 acagccgtta cagctgatac tttctggaag tcacttacgt tgattttgat ttttttgttt
    42301 tcaaacatct ctttctctct ctttttcttt attaccaaag ttctttggtt aatttaggtc
    42361 cttcgactgt tttcaataga atttttttct ctggctctct caaatactct tttagtgaaa
    42421 aaccgccttg ccgagtcact acttgggtat cgaattgctc aaaaaaagca ggatgcgatt
    42481 gaattacctg aattggcaat tcaatcggtg aatttgcgta cagcagcaaa tcaaaatcac
    42541 tggtttcttt tactgccgga atcccagtcg ataattcaaa tcctaagctg ccgccgattc
    42601 cccatttaca tcctgataat atgtttctag cagcatgaaa ttgctgataa acaggataat
    42661 ccatgatttt ctcttgtcta tacgattctt gttcgatgat gtcccacgga tgaatgactt
    42721 gtttgacggc atgttttgag atttctgttg accatctctg attgcgggca ggtccacgta
    42781 aaccaatcgg tagtatttct gcgtgatccg tcgacgcacg acgaactgtt ccgaacatct
    42841 tttcagttag aacgacccat tcaggcaagg tgaacccttc tatttcatgt ttatcaaaaa
    42901 ggacaatatc atgtgcttga atcatttttt atgcatccca ttcttcgtcc attttggcac
    42961 ggactttatt tgtttcgaca cgtccgaatg ttttggctgt ttcgtttgtg tatcgatttg
    43021 tcagcatcgt gtcatttgta ttgcggacat cttgaatggc ttcattgatt gcttcgatga
    43081 ctttgctgac attttgttct gaagcagctt ggtcggtgat atcttctagg aaatgataga
    43141 tcgctcctag ttttgtataa ttattgatat cgtaagcgat agctggaact ttttcagcag
    43201 ctgcttcgat ttcagcaacc gaacgttttg tcacacgtgc agcactttct ttagacatcg
    43261 cttggacggt aatttcttta ttgtttaaag caatcaagcg gcttgattgt aatccatgtg
    43321 ctaaaaatgc acctgaaata gcatttcctg caatcaatcc aataactgga tgtccagctt
    43381 gacggagttt tgcataagca gcagcactat tagccaaagc cacatggata ccgatcaatt
    43441 cttcgttata gccgtatgct tggcttggta catcgatcac aacgatgatt ggccgtttca
    43501 cttttttgtc tttatcttct tcgtaaacgt gatccaaaat ttgtcccatg cggaaacctt
    43561 caactaaacc aacttcaccg tttcgtacac ggtacatcgg gtttttagga tcaggtacga
    43621 ttgatgtcac gatggcttct tctatgaatt tacttgattt accataatac acagtactaa
    43681 tagaagaagt tggatttttc atgccagtta gtgccgcaaa ccaatcgtaa ccaacacttg
    43741 ctgcagctgg ttcatttcct tctgctactt ctggtaagtc aattgatttt gcttgatggt
    43801 tagcaattgc ttcattgtaa tcgtcaatgg ccattggttt tgaaagatct aaagaatcaa
    43861 gtaatgataa ataaaatgct gagctttcac tgcgatgcgt gtcttttcgt tcgtccattg
    43921 ctgcgatgac agcagctttg attgcttcta cactatcagt cactaattca tcaatgattt
    43981 ttgattctac ccgttgacga gatcctaatg tattccaaat caagtctttg tcactagaat
    44041 caaattctcg aacgcctgct tcttgttcaa taacttctgg tccattcaaa ccgactctcg
    44101 ctttttttgt tgcgattaaa taactcatta atgctgaagt aatcgacata ccgccaaatg
    44161 atcctacacg tcctggaatc aaaccgatga ctggtacata ttgttttaga gcaatgatta
    44221 catttccgat ttctgaaata gataacagtc cgtagtttgc ttcttgcaaa cgaacaccac
    44281 ctgtatctag tacaatgact ggaaagatct catttccttt tttattttct tctaacacat
    44341 gttcaagtgc agcaacgatt tttgcaccgg aaacttcacc gataccgcca ccttggaatt
    44401 ttccttccat agcgattact actgcttgtt tgccattgat cgtgccacgg ctgacaacga
    44461 tcccgtcatc actttcaggg acgattcctt gtgctggtaa acttggcgcg atgatttgat
    44521 cgaatggatc aatcaattca cgtccattgt caagtagagc gttcactcgt tctctggcgt
    44581 ttaattctac aaaactattt ttcatctgaa agttcctcca ttgcttgcgt caatcgtagg
    44641 tacacaactc ctggtgtagc accgaagtca ttgatcacga tatctgctga aattggataa
    44701 cgatcaaaaa atctcgttaa tacattgttc catacttcgc cgaagccatc gcttcctgta
    44761 caaattttta cagtagattc tgttttttct gttggaaaca tcaagatttc caaatctcct
    44821 gaaccgacca ctcctacatg gattttctta ttgatcggct gtttcgttgg atatttataa
    44881 gctaattgtt ccatggatct ctcccctttt tgtaaaacag agcatacacg ttctgcttat
    44941 tcgtctttct tcttccatct gggtaaatgg cgacaaaagg tttttctcgc caattttatg
    45001 accaagttct gaatttcatt ggtggttcat acaggccatc tgaccattcg atcaaatcat
    45061 cgatggtttg tgcagctaat aatgaacgtt tcgcttcact acgtttgatg cctagatctt
    45121 ctggtaattt cacgatgccg gctgcacgca agctatctag tgattctttc gtgctagtca
    45181 ttccaagagg tgtaacaccg ccaattgctg caattgctgc ttgtcgttct tcttttgttt
    45241 ttgctttgta caaataagca attccttctt ctgtcacgat atgtgtagta tcttcgctat
    45301 aaatcatcac tggtgcattg gctaagcctg cttgtttttg aacagctagt gcatctaatt
    45361 cttcgacaaa gactggtttt ttatttgaac catatgtttc aaccatttga acaactaatt
    45421 tttggccttt ccctaatgga tcatcattgt cgcgaagtga ttgccatgct ggtgtagaat
    45481 gacgacgacc gccaggatta tgtcccatgt taggcgcacc gccaaaacca gataaacgtc
    45541 cttttgttac ggtagaagag tttccttcgt aatctaattg caaggttgaa ccgataaaca
    45601 tatcgatggc atattgtcct gcagcttgtg ataatgttcg gttactgcgc atcgtgccgt
    45661 ctttaccgac aaaaaagaca tctgggcgag cagcaatata tttttccata ccaacttcgc
    45721 caccaaaact atggatgctt tcaacccaac ctgattcgat ggctggaatc atcgttggat
    45781 gtgggttcaa tacccagttc ttcgcgattt tcccttttaa ccctagtgat tcaccataag
    45841 ttggcagtaa caattcgata gctgctgtat tgaatccaat accgtgattc aaagattgta
    45901 cgccgtgttc tgcgtagatt cctttgattg ccatcattgc cattagaatt tgcaattctg
    45961 tgatgttttg aggatctcgt gtgaataaag cttctaattg ataaggttta tctgctttca
    46021 cgacaacgtc tacccagtca ccagggatat ctacacgtgg taaagaatcg acaacttcgt
    46081 taacttgaac gatcacgata ccatctttga aagctgcagc ttcgatgatt gttggtgttt
    46141 cttccgtatt gaaacctgtg tacaagttgc cgtctttatc tgctttatct gcagctacta
    46201 aaacaacatt tggaatcaaa tcgatgaata agcgtccata aagttccaaa taagtatgga
    46261 tatcacctaa tttcattgtg ccgtcttcaa tcatttgggc gacacgcaag ctttgtgcac
    46321 cagcataaga gaaatcgatc ttgcttgcga ttcctttttc aaaaatattc aaatgttctg
    46381 gtcgtgagat actagacatg atcaaatgca gatcattcac tttttctgca tctacttttt
    46441 caagactttc tgaaagaaaa cttgcttgtt tttgattgtc gccttctaat acaactttgt
    46501 cgccgggagc aatcagtttt tccattactt ctacgatttt ttcagtttct acatacttgc
    46561 cattgagcaa gttgctgatt ttttccaatc gctgcgcttt tttttcgcgt ttagaagccc
    46621 agttgcggtt tggtttttcc ataattagtc atctccattt ttgatataga aacgtggcgt
    46681 catcaatacc atgttggtct agattgatcg tacggatccc tttatctaca ccaaattttg
    46741 tatgaatcag ctttgtcaaa gtattgccgg ggggaaattc aatcgtcaga tccattccta
    46801 attcttttgc caagtccatc atttggttcc actgcacagg gaaagctagg ttttccaaca
    46861 aatctttacg aatctcttct gcgtcctgag tggctcttcc atccgtattt tttatataca
    46921 aacatctagc atcagataga tggacagact gcatatacgg cttgaatttc ttgaccgtat
    46981 cattcatcaa gagacagtga gatggcacag ggactttcag cagcttcgct gtttgcgctt
    47041 ggtgccgttt ggcttctgcc aacgtaacgg ataaaccttc gatactccca gataacgtat
    47101 gttgcatcgg acaattttca tttgacagat aaactggatg gtcaggatca aatgtttttg
    47161 ctacaatttc ctcagcttcg cttctcgtca cacctactac tacgcccatt gcataaccgc
    47221 ttggataagc ttctttcatt aagactgcgc ggtgatagac tagttccgct gcattctcaa
    47281 atctcaaaga tcgacaagca acagctgcac aaaaagcacc aatcgaatgt cctgctgcaa
    47341 tatcaggctg aataccgttt ttttccattt catctaaata aaatactgct tttgtcagta
    47401 atgctagctg gataaaaacc gtatcttgat agttttcttc tttatcaact aaccgatacc
    47461 ccgtcacatt ttctaaacgt tccatgtaca tttcagggat atccgctaac atccctaact
    47521 tctgactgcc ttgtccagga aataagaaac ctgtactcat gagcctcgcc tcctcagcac
    47581 ttactttttc cactgctatc ttagtcaatt tctgctataa taaaaaatgc caaaaaaaga
    47641 aaaaaccata acaaaaaggt atggatggga tggataatta tgaaattgct tcatctgcag
    47701 tattttaaaa aagtattcga aacaaaaaat gttacccaag cagccaaaga attatttatt
    47761 tctcaacctg ccttgagccg tgcgatcaaa catttggaaa acgaattagg tgtccctttg
    47821 ttttaccata gcggcagaaa cattgagacg accgtttatg cagaagaatt ttatccatat
    47881 gcagtcaaga cattagacac attagaagaa ggaatcaaat tattgtcttc ggtcaatgag
    47941 aagatcgtta cttccgtcat attgtattta gaggtagcct ctgtttctat tccgaacttg
    48001 gtccgaattt ttatggaaaa gcatcctgat atccaattaa cgatcatgca acatcattta
    48061 ccagatgata ttaccgatcc tgtactgtac atcacttcgg agatgaagcc tggcatgacc
    48121 aatattccta tcatgaaaga acctgtttat gtcgccattc ctaagaaaca tcctcttgct
    48181 aaaaaagaac atctgcaatt agaagatatc caacgcaccc ccatcttgat gctgtcaaag
    48241 agcaacgcct tccgtcatac actggatgca gcacttgaag cgaaaagtat cgaattgacg
    48301 atcagttcga cgacagatga tcctgcgaca ttacgttcta ttgtcaagca aggattagct
    48361 gttagctttt tcccaaaagt ttcttggtct tacgataaaa atgatccttt cgtcttgcgg
    48421 gaaatcaaag atttgccctt gacacgaaca atttatttat cttctagctt cacagaagat
    48481 catcctttga tcaaaatgat tgcaaaaacg atgcgggaat tttatttagg aaaataaaaa
    48541 aaaaagagct gggacaaaat tcttgtcgca gctctttttt cgaacaaacg gtgttccaga
    48601 agtaatttct tagaaaataa gccgaaattt cccaaaaatt tgagaagcaa ttttcgtaaa
    48661 ttccttctta tttcttgaag ctaaacattt ctgtcacaac ctcttctcta ttgtcctaga
    48721 cattcattgc gttgatttaa aacttccatc aatcaaatct gctagttcat ctagcttttc
    48781 tattttacgt agatcaatag agatccaatg ttctttattc atgtgatagg ctggaaaata
    48841 cccctctttt cctcgcaaaa ttccgccaag ttcaggatcg accttcaaat caagaatatt
    48901 gatgatccta tggtcacttg catcatcata gattttttct tctggaacag acatcaggac
    48961 cccaaaccat tttttgcttt tttgatgtct gaatacgcaa taatcaggaa attttttaaa
    49021 aatatattct ggagacaaat tgtatgtttc attgatgtga tcgattagtt cttttttcgt
    49081 tagcatctgc ttccatctct ttcttattga ttcgctttat tcaaagatac cacgtaagaa
    49141 tagaaaaatg aatacaagta gttgctaatc aaatacttcg atatttttct tttttatcaa
    49201 ctcttaaatg ttcaatccgc ctgtgttggt cttgctacca tctactatta aaatgtttat
    49261 tattgtttgc ccacctattt tttgatttta gtggtatcat ttttcaagtt catgatgaaa
    49321 ttcttatcat cagtaaatgc tccagaaaaa taaacaatga ttgaaaaatc agttgaaact
    49381 tgaccaccgt ttattttctc cgtattctta gaatagaagg gagtctgatt gatgtattca
    49441 ctaatgaaaa aaatcattac cgaaaaagat attcgacgtc atgtttcttt agtagaacaa
    49501 ttgctgaatc atacaaagat aaccgtaaat gaattagctg aaatcatagg gacaaccgaa
    49561 cgaacgattt tttctgacct tcaatctatt cgctctcagc tgcccgaagg ctgggacatc
    49621 atcagcgatc aagcaggaat cagtttgcaa aaccagcaaa atttattaac aaatgacctt
    49681 tgggagattt ttttcaaaca atcagtcagt gtcgaactgc tgaaaaatct gctttttaca
    49741 aaaaaagttg ctgtccctga ttttctagcc gaccatgggc tttcttatgg gacattgaaa
    49801 agacatgtga ctaaaatcaa tcagcgttta gcaagttacg acttgcagat cgatttgacg
    49861 aaatatacag cttgtattct tggaaaagag cgtgcgattc gtacttttta tcatcgattg
    49921 ctgattcctt tcactcataa caactatttt ttcgaggatt attcgatcca tgaatcccat
    49981 tattttcaat ttttaagaaa cctcagtcaa actgaactag cggttgaaac agaagagatt
    50041 ttcggaacat gttggttctt tatcaatacg atccgtataa aagcgaattg ccgattagat
    50101 tcttctatcc ataccaacag cacactctcc tccctgtatg actctgcatt gaaaaaattg
    50161 tatttaacgg aaggtattta tcttaaagac actgagcttt cttttgcttc tttctgcttt
    50221 ctggaaagtt ggaattacaa caataactat ggacaagaaa ccgctcgctg cctgcatcat
    50281 tctccatttt tagaggtcct cgaaacgttt gtagaggaac tagcttcaga gctgtctctt
    50341 gatcaactaa aaaaaacatc tctgactgat aacttggctc tcctcatatt aaaatatcat
    50401 gaatcgccga tattgattga agcattggac cgtcaatacc atcgtttttt agaaacatat
    50461 gatcagcaat atgcacactt gtatcaacag aaagagaatt tactgaacga tctaaaaaaa
    50521 gaaatagaaa tcaatgaacc aaattacttt ctccaactct tgtctctact gatccaacaa
    50581 acgatttttt ctatcaaacc gaatttgttc aatgtctatt ttattttcca aggagaacct
    50641 tcatggaagg cgttcttgca gcaggaatta aaagattact taggaaaacg tgtctgcttc
    50701 ttacctatcg aactaaccga tctttccact atttcttttg aaaaaacaga tatcttgatt
    50761 tccaatttcc ctctagattc tctagatatc cctattgtat acatttcttc tattccaacg
    50821 aaaaatgagc tcaatcgttt gactgaactc actttcaaaa cttttttata gtaacggcta
    50881 gcgactgtat tctctagctg ttttttcttt tttgcgaaac agtttgttca tgatcccttt
    50941 ttctatttct acaactaaga atagaagaat cacatttgct ccaatcaata actgttgtgt
    51001 catcgttaaa cttactgtac cgatcagctg ctggccgact ggtagaaaaa taaccatccc
    51061 ttgcaataaa gcaaggatac ctaatgaaag aaaaagcgct ttgttttgca aaagaccttg
    51121 gttcaatgac ggcttggaca actctctgca attgatcatg taaacagctt gagctaagac
    51181 gatattttgc agaagaatgg tttgctgtaa gctttgccct tcaaactgca tagccaataa
    51241 ataggcagga accatgatca gtagggagac atagatgatc cgaaacaaat gataaccaga
    51301 taggattcct tgctttggat ctcttggtgg gcgcttcatg atttctggat ctggcgactc
    51361 gaaccctagt gcataagata aagtgattgt cgttaccata ttgacccata aaatctgtac
    51421 tggggacaaa ggtagcgggc gattcagcat aagtgcccaa atcaagatca atccttgagc
    51481 taatgcagtt ggcaagaaga aattgatggt tttttgcaaa ttatccaaaa tccgccggcc
    51541 ctcttttacg gcttttgcaa tcgtatggaa attatcatct cctaaaacca tatcagctgc
    51601 ctgtttgctt acttcactac ctttgattcc catagcaatt cctacatcgg atcttttcaa
    51661 agcaggtgca tcatttacgc cgtctccggt cattccgacg atttctccat ttttctgcaa
    51721 agctttaaca attcgcagtt tatgttctgg tgtcgttcga gcaaagacat cgactattgg
    51781 cacttgctga atcaactctt catcagacat aagatccaaa tcgatccctt ctaaaacttt
    51841 ggcagtatgt tttaagccaa tttgttccgc aatcgctttt gctgtgtctt tgtgatcccc
    51901 tgtaatcatt ttcactgaga tccctgcttg ttggcattcc tttacagctt tgactgcact
    51961 ttcttttggc ggatcgatca atccggcgat ccctgcaaaa gttaattggg ttaaagtctc
    52021 atgcgtgagt tcctgtttcg aatctacttt tttataagca aatcctaaaa cacgctgacc
    52081 ttttctcgca aattctgcag ctgtctgact ccatgcttct tgttctgcag aagaaagagt
    52141 tgatttagca aataatactt ctggtgcacc tttgacgtaa atcaccgctc ctttttcctc
    52201 cgcatgcatc gttgccatat atttgtattc agaactgaac ggaatctttg cttctatttt
    52261 tttcaaaggt agattggctg tttctgtgtg tcgaatcagc gcaacttctg tcggattgcc
    52321 acttaaattt tctgttttct gttcttcttt tgtttttaat tcctgacagt ttttcataat
    52381 ggacaacacg caggcttctt cttttgcagc tacatccaca actgtcattt cattttttgt
    52441 caatgttcct gtcttatctg aacagatcac tgtcatagaa cctaatgttt ctaccgaagg
    52501 aagtcctttg atgatcgcct tttcttttgc catttcatgt actccaacgg atagaatcat
    52561 ggtcaaaaca gctggcaatc cttcgggtac cattgacacc accaaagcaa tcgtagccga
    52621 aagaagaata tgccaatcca taccatatcg aaatgtcgta aaaataacta agaataggct
    52681 caaacaaaga atgccttgaa aaatttgttt gtttaatcgg ttgatttttt taatcagagg
    52741 cgtcgtttgt tgttcgaccg attgcaatgc ttgattgatt ttaccgattt ctgtcgtgtt
    52801 tcctgtttct acaacgacac cgactgcaga accagattgt acaagcgttc ctgaaaaacc
    52861 catatttttc tggtcgccga tcgattcttc ttctgtcaaa acggcatgac ttttttcaga
    52921 tgcttctgat tctcccgtca aaatcgattc ttccactaat aaatcatgtg cttcgattaa
    52981 tcggatatct gctggaacga catctccggc acgtaagaca atgacatctc cttttaccag
    53041 ttctttcgca gggatttttt cttgcatccc gtttgatagt actatcgctt cttgtcccat
    53101 cagctgcttc aaaccattca atgattcttc cgcttttctt tcttgccagt aactaacgag
    53161 tccattgacg atcactacga ataaaataat acttccttca atatattcac tagaagcaaa
    53221 tttcaaaaaa gaggcaatga tcaacacgat catcagcaga tcagtaaaat gctttgctat
    53281 ttttttccat cgttttgtct gctgccttgc ttttatttca ttatatcctt gatcagtcag
    53341 gcgtttctgt cgttcttctt gcgacaagcc ttctgttgat gtttgatagt ttttcaatac
    53401 atcttctacc gtcaatctgt accagttcat cctatcccct cctgtttaat tgatactgtt
    53461 attatacgca gggaagtttt ttcaggcgta tcgattattt caggggggtc tgaaataatt
    53521 cgttttgcaa aatgtatctg ggcctaactg atatttctat tgcttattct tttatataca
    53581 aaatttaaga atacaaacca gataaccaat aaaaaaggaa taaagcgtgt cgtgctttcg
    53641 aataacaagc tgaaagcaat tgaagtaaaa aacaccaaca aagcaagatc tttatatctt
    53701 tgtgtagtgt tccgtttctt ttctttaata aattgaacat gtgcacacaa gatcgaagac
    53761 cacacaaata gaaaacaact cgttgtcatg cctgccaaaa gctgaaaaaa caatcgacta
    53821 tcagctacaa aaaaatgtag acaaagaccg gcaaaaaata acattcctgt aattaaaata
    53881 ccgtgtaaag gtacgccttt cttagaaagc gcacaaaacc aatggggcaa acttttttct
    53941 tttgctgttt gatataaaat cctgctagta ctgtagactg cactatttgc tgaggacaaa
    54001 gaagcaacca aaatcattag attgacaatt gctgctccgc cttggcaatg gatcacttgc
    54061 agcatagtca ctaaaggact ttcttttaaa tctaaacaat gccatggaac aacaaataaa
    54121 atcgacaaaa tcgttcctat atagaaaaag agaatacgcc aaggcagaca gttgattgct
    54181 tttttcaaat tcttttgcgg atcacttgtc tcgccaacgg ttaagccgat catttcaatt
    54241 ccagcaaaac tgaataaaac cataggaaat gagactacca ttccagcaaa gccaaacggt
    54301 agtaattcat cgatctgtcc aaaattttct ttgattccga tagactgggt atgaaaatct
    54361 gagacactca atcctatacc caataagata aaaaggaaaa tccccaagat cttgatgatt
    54421 gagaaaaaag attccaattc accaaaccac ttgactgcaa caaggttgac tgccattaag
    54481 atcattagtg cgctacctcc gcttatcaaa gctggtacat gtacaaacca gaaatggata
    54541 tacaatccta ttgctgttaa ttccgcaagt ccagctccaa gccagcaaag ccaataagtc
    54601 caattgacaa aaaatgccca ctttttgcct aaataatagt caacgaaatc actgatagag
    54661 ttaaatgtac tatttgctaa aaataaatca cccatggccc gcatcaaacc ataaatagcc
    54721 aaaccgcata tcaaataaac gagaataaca gctggccctg ctgatgcaat tgcctttgct
    54781 gaacctaaaa ataatcctgt tccaattgcg ccacctaatg cgattaactg gatatttcga
    54841 ttagttagag tccgtttcat tgccggtttt tccatcaagt tcaccttttt ctttttcaag
    54901 cagcatggcg acttcctgtt cccaagaagg tttctttatt ttttcaatac agagtggtac
    54961 tacaaacaaa atgacactga tggctgtgac tgcaattaga ttgatccatg ttccagcttt
    55021 catcgtcagt ccgacagaaa atccgattgc cgcaaataaa acaataacga cagtccaaac
    55081 tccaagattg ctctttttct ctttttttcc aattctaaat ggtcgattca actttgcatg
    55141 tgtataacgc aatctcaaaa ctgcgatccc catgatgaca tagacgatac agtagataac
    55201 agtagttgaa tttacgagca tcaaaaatgc ttgattgacg ccaggaatca gcaaataaaa
    55261 aagtgcaaag attgagataa tgacagactg agtcaaaata acggatttgg atacgtcaaa
    55321 ttgattcgtc ttccaaaaat tgaattttgg tggatacgca ccttttctag cactttctgt
    55381 cactgtcttt gctggtcctg aggcccaagc agataattgg actgctacac caatgaagac
    55441 aagtaaacta aacagattca caataagatg tggcaaacca agaatttggc aataaatcga
    55501 aatagattgc gcgatattat tcaattccat ttgtcctttt ggtacgacat ttgctacaac
    55561 taaagcattc aaaatattga cgaaaaatat aaaaattaac gcggcaaata ctccgcgaat
    55621 atacgtttta actggatttt ccaatcgagt gatatacaca gaggacattt cgataccagt
    55681 aaaaataaac atgatcggcg caaaataaac aaatgaggat gcagtcgtag tatctggtat
    55741 taatttgtcc catgaaaaac tgcctaatgt tccggttggt acgatcccaa ctttgatcca
    55801 cgcagcaaac cctaataaga gcatcatcgt caaaggcacg tacaatccta accaaacacc
    55861 gacttttccg ccgatttttg ccatatcaaa tttcaaattc aaaaaagtga tgatccaata
    55921 aacgactaaa atgacaacta atgtaaattt gctgttcaaa ccaagcggca cattatcgat
    55981 aatattacca aacaacggag cgagtgctga agcgaccatc accatcccag gaaacatttg
    56041 gacccataac agccatgaga caacgaaccc ccattttttt cctagagcat tcgataccca
    56101 aagttctgga ccaccttttt ggggaaacat tccagcaaat tcaccagaga tcaatgagat
    56161 tggaaaagcg tacaataata ctgcaaccag catgtaaaaa atcatcgtcc agctcgttgc
    56221 tgctacatct ggaatatttc gtacacttgc tacaagggcg caggtcattc cgataaaggt
    56281 catggtcgtg atttgctttg atgaaccagt atctttcatc tttattcctt ctttcaatag
    56341 cgacatatgt ttttgttttc ttttattttt gttcaagaac agaaatgaca aggtttcatc
    56401 gattcagtac aaaacccagt atcaccgaaa catccattga acgatcttag tgaactgggt
    56461 ttgatacatg aaacgaacga tccaacttat agaacgtttt ataaaaaaga atatcttggc
    56521 gaggaatata tgaaaaacag ggggaaatac aattgttttg atgaagcaaa taattgtctg
    56581 ttctccatat gtctcttgtt attctttcac acaaaaatca gttctattgg atgatttcgt
    56641 ttagtttctt ttccattgct ttggcgattt tttttgtata aaaattgaaa attcgatcat
    56701 cattcaaata aggagtcatg atagccgcac gtagcagagt gattttctgt tcttttttcc
    56761 attcttcttt cgttagaccc attctttcaa caaaacgaat cggagaatca ccgtaatctt
    56821 cttgtgtaaa tgtagtatga gaagtaataa accgttcacc ataaacatcg ccatccatat
    56881 aagaagaccg atcgaacatt ttttcattta attcattgat agcgttcaaa tctgtacagc
    56941 cttgttcttt gaatacccaa ttgaccatat taaaatcagg atgattcaat ggatagactt
    57001 cgattgtttt tcctttaaca gtaaaagtca gatgatcgag aaattctctg aatctttgag
    57061 ctgcctcgat tgaagcccca atcaattgac catatccggt cacattcaat ggcaatacac
    57121 gatgtgctgt ccagactgcg gcggcggttg ctccagcttt tgatccttcc aaaatataag
    57181 ctcctaacat atctggagcc tttactgatt tttcaaacac atatggcgcg aaataagaaa
    57241 tgatatttct catattttta tgtttgatta cgattcctcc agcagcataa gggacatagc
    57301 ccatcttatg aggatctatc gtgacagaat ccgcttctga aatagctctg aacccttcat
    57361 atacctcttt gtcaatcgtt acgcagtggt ggaaaacatg atgttcttca aagaattcag
    57421 ctaaggaagc atacggcaca aattctcctg cttcgtttag aaacagtgaa cgcgcatagc
    57481 cgccatatgc ggcatcaaca tgtaaataga aatagatccc ttcatctttt aacctttctc
    57541 ttaattggac gattttatct acactgtcta cttgaccttc ttctgtcgtt cctacgacag
    57601 caaccacacc tagaattggg attttttgtc cagctaattc acgaattgtt ttttccaaag
    57661 cattaatatc cattcgataa tcctcttgaa ctgggatagc aaccatctga tctaagccga
    57721 ctccgcaaat atctaacgcc ttcatccaag aatagtgttt tgtttgaggg accagccatt
    57781 tccctaatct ttggatattt tttccgctac gggaagaagc tgctttgacc tcatccattt
    57841 cttcgtccgt aaagctttcg gtcatttcca aaatttcttc aacagataaa ttcaaaagtt
    57901 cccactcaga catttttttc actttttcag gcagcacttc cttgacagct aaaggaatcg
    57961 atttgatgca acgggcgtac cataatcctt caagattagc aatagaacca tctgctgcaa
    58021 tgtgtcccca tccgtctgtc atattgaata atgaagcaaa atcctgtcct acttctgctt
    58081 ccatttgcga agttgccata gaagattcca atgcaacatt atttgggttc cacagcatcg
    58141 catagttgta agctaataaa gcaggcatca atgtttcagc attcatttgt ccccagtaac
    58201 ggcctgctga atgccatggg atcgatcctg cgcgtaatcg ctgactgact tcatccaaga
    58261 cctctatcat atggtcgcgg gtggctagat aatctggagt gagcttatct ccttcgctaa
    58321 ttgcaggcat atctgaagga atataatttt ctctccatcc aagatgttca tctaccatct
    58381 tgttcaacaa cattttataa actggtccat tttcagcttt gtcgcctata aagacggcct
    58441 tgatatccat atctttcatt tttcttccct ccatattttg ttggtcaatg tcttgcagat
    58501 acatgtgttt atctgtaaca cctcttcact atagcgccaa aaatgagaat ttttttttta
    58561 taacatcaaa tccacgtaga tttttacaaa caaaaataaa taaagtcgga tttttctgat
    58621 aattcaacgc aaaaaagcga acaatttttc aataaatcag tcttctaaaa tagataacct
    58681 aaaaaaagcg aacaaacaaa taaaatgact gtccttcatt cactaaacat atttttattt
    58741 ttttatattg ttaaaaattc gcaatttatt ttttaataga attatctgtt ttccaaattt
    58801 tataacataa aaaacctttt ttaacggcat ttatatgctt tttcagaaat taactgatga
    58861 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
    58921 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
    58981 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
    59041 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
    59101 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
    59161 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
    59221 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
    59281 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
    59341 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
    59401 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
    59461 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
    59521 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
    59581 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
    59641 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
    59701 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
    59761 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
    59821 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
    59881 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
    59941 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
    60001 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
    60061 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
    60121 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
    60181 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
    60241 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
    60301 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
    60361 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
    60421 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc
    60481 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
    60541 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
    60601 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
    60661 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
    60721 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
    60781 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
    60841 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
    60901 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
    60961 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
    61021 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
    61081 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
    61141 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
    61201 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
    61261 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
    61321 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
    61381 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
    61441 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
    61501 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
    61561 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
    61621 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
    61681 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
    61741 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
    61801 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
    61861 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
    61921 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
    61981 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
    62041 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
    62101 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
    62161 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
    62221 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
    62281 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
    62341 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
    62401 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
    62461 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
    62521 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
    62581 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
    62641 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
    62701 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
    62761 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
    62821 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
    62881 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
    62941 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctatcgtcg tgcatcatca
    63001 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
    63061 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
    63121 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
    63181 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
    63241 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
    63301 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
    63361 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
    63421 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
    63481 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
    63541 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
    63601 ttttgaacac cgtttaccaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
    63661 acccaagcta aaactgcttc ttatgttttt gacttggttg gacttattac cgcagatgca
    63721 agcttttgaa caccatttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
    63781 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
    63841 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
    63901 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
    63961 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
    64021 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
    64081 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
    64141 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
    64201 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
    64261 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
    64321 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
    64381 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
    64441 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
    64501 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
    64561 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
    64621 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
    64681 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
    64741 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
    64801 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
    64861 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
    64921 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
    64981 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
    65041 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
    65101 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
    65161 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
    65221 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
    65281 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
    65341 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
    65401 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
    65461 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
    65521 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
    65581 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
    65641 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
    65701 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
    65761 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
    65821 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
    65881 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
    65941 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
    66001 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
    66061 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
    66121 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
    66181 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
    66241 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
    66301 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaacg gaaaacaatc acttgttctc
    66361 cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc
    66421 ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca
    66481 cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc
    66541 ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag
    66601 accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca
    66661 aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc
    66721 atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca
    66781 cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg
    66841 ctatgcccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc
    66901 atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata
    66961 aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc
    67021 actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact
    67081 aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact
    67141 gccggtggca caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca
    67201 aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata
    67261 cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg
    67321 tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga
    67381 atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa
    67441 attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat
    67501 gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc
    67561 cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat
    67621 ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag
    67681 atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact
    67741 gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc
    67801 tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc
    67861 taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt
    67921 tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg
    67981 tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt
    68041 caccattcat catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg
    68101 ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt
    68161 ctacattttt agattctagt tgattatccg catctgaagc agatacagca aagatgtctg
    68221 cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca
    68281 ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta
    68341 tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga
    68401 tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact
    68461 aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga
    68521 cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta
    68581 ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt
    68641 gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg
    68701 tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat
    68761 gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc
    68821 ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg
    68881 ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag
    68941 tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc
    69001 aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc
    69061 tcatccatat actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg
    69121 atattatgat aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt
    69181 tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt
    69241 tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga
    69301 taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct
    69361 gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag
    69421 tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca
    69481 gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag
    69541 ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa
    69601 actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca
    69661 gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt
    69721 accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact
    69781 tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc
    69841 aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc
    69901 tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg
    69961 atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat
    70021 tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca
    70081 tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc
    70141 gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg
    70201 atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg
    70261 cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt
    70321 tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac
    70381 tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg
    70441 cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac
    70501 tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc
    70561 ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc
    70621 catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc
    70681 ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt
    70741 caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag
    70801 caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat
    70861 gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc
    70921 cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac
    70981 ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat
    71041 agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc
    71101 caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt
    71161 aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag
    71221 cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac
    71281 atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg
    71341 tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt
    71401 aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt
    71461 attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc
    71521 agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg
    71581 cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa
    71641 ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct
    71701 cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac
    71761 gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc
    71821 tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg
    71881 attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa
    71941 tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc
    72001 gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc
    72061 atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct
    72121 tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc
    72181 cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc
    72241 accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct
    72301 cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact
    72361 agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat
    72421 tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc
    72481 atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
//
LOCUS       DACDHL010000005        67564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDHL010000005 DACDHL010000000
VERSION     DACDHL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.005"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..103
                     /locus_tag="IV228_000337"
     CDS             <1..103
                     /locus_tag="IV228_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP8716284.1"
                     /translation="IVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            279..1022
                     /locus_tag="IV228_000338"
     CDS             279..1022
                     /locus_tag="IV228_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716285.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1015..2595
                     /locus_tag="IV228_000339"
     CDS             1015..2595
                     /locus_tag="IV228_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8716286.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2846..3325)
                     /locus_tag="IV228_000340"
     CDS             complement(2846..3325)
                     /locus_tag="IV228_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8716287.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3341..4093)
                     /locus_tag="IV228_000341"
     CDS             complement(3341..4093)
                     /locus_tag="IV228_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8716288.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4210..4280
                     /locus_tag="IV228_000342"
     tRNA            4210..4280
                     /locus_tag="IV228_000342"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4242..4244,aa:Gly,seq:ccc)
     gene            4641..5366
                     /locus_tag="IV228_000343"
     CDS             4641..5366
                     /locus_tag="IV228_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8716289.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5621..5887
                     /locus_tag="IV228_000344"
     CDS             5621..5887
                     /locus_tag="IV228_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8716290.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5884..6315
                     /gene="ruvX"
                     /locus_tag="IV228_000345"
     CDS             5884..6315
                     /gene="ruvX"
                     /locus_tag="IV228_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8716291.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6340..6645
                     /locus_tag="IV228_000346"
     CDS             6340..6645
                     /locus_tag="IV228_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8716292.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            7214..8050
                     /locus_tag="IV228_000347"
     CDS             7214..8050
                     /locus_tag="IV228_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8716293.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            8114..8545
                     /locus_tag="IV228_000348"
     CDS             8114..8545
                     /locus_tag="IV228_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716294.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(8607..9929)
                     /locus_tag="IV228_000349"
     CDS             complement(8607..9929)
                     /locus_tag="IV228_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8716295.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(10083..11723)
                     /locus_tag="IV228_000350"
     CDS             complement(10083..11723)
                     /locus_tag="IV228_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8716296.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12056..12526
                     /locus_tag="IV228_000351"
     CDS             12056..12526
                     /locus_tag="IV228_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8716297.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            12748..13851
                     /locus_tag="IV228_000352"
     CDS             12748..13851
                     /locus_tag="IV228_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8716298.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            14135..14815
                     /locus_tag="IV228_000353"
     CDS             14135..14815
                     /locus_tag="IV228_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8716299.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            14905..15939
                     /locus_tag="IV228_000354"
     CDS             14905..15939
                     /locus_tag="IV228_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8716300.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            15953..16288
                     /locus_tag="IV228_000355"
     CDS             15953..16288
                     /locus_tag="IV228_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716301.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            16272..18752
                     /locus_tag="IV228_000356"
     CDS             16272..18752
                     /locus_tag="IV228_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716302.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            18808..20367
                     /locus_tag="IV228_000357"
     CDS             18808..20367
                     /locus_tag="IV228_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8716303.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            20364..21728
                     /locus_tag="IV228_000358"
     CDS             20364..21728
                     /locus_tag="IV228_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8716304.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            21899..22969
                     /locus_tag="IV228_000359"
     CDS             21899..22969
                     /locus_tag="IV228_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716305.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            22984..23652
                     /locus_tag="IV228_000360"
     CDS             22984..23652
                     /locus_tag="IV228_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716306.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            23672..24250
                     /locus_tag="IV228_000361"
     CDS             23672..24250
                     /locus_tag="IV228_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8716307.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            24651..25562
                     /locus_tag="IV228_000362"
     CDS             24651..25562
                     /locus_tag="IV228_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8716308.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(25650..26567)
                     /locus_tag="IV228_000363"
     CDS             complement(25650..26567)
                     /locus_tag="IV228_000363"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8716309.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            26769..27206
                     /gene="zapA"
                     /locus_tag="IV228_000364"
     CDS             26769..27206
                     /gene="zapA"
                     /locus_tag="IV228_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8716310.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            27265..27813
                     /locus_tag="IV228_000365"
     CDS             27265..27813
                     /locus_tag="IV228_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8716311.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            27928..30288
                     /locus_tag="IV228_000366"
     CDS             27928..30288
                     /locus_tag="IV228_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8716312.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            30375..30689
                     /gene="trxA"
                     /locus_tag="IV228_000367"
     CDS             30375..30689
                     /gene="trxA"
                     /locus_tag="IV228_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8716313.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            30871..32649
                     /gene="uvrC"
                     /locus_tag="IV228_000368"
     CDS             30871..32649
                     /gene="uvrC"
                     /locus_tag="IV228_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8716314.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            32794..33693
                     /locus_tag="IV228_000369"
     CDS             32794..33693
                     /locus_tag="IV228_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716315.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            33686..34948
                     /locus_tag="IV228_000370"
     CDS             33686..34948
                     /locus_tag="IV228_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716316.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            35109..37007
                     /locus_tag="IV228_000371"
     CDS             35109..37007
                     /locus_tag="IV228_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8716317.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(37337..38056)
                     /locus_tag="IV228_000372"
     CDS             complement(37337..38056)
                     /locus_tag="IV228_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8716318.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            38393..38725
                     /locus_tag="IV228_000373"
     CDS             38393..38725
                     /locus_tag="IV228_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716319.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            38712..40715
                     /locus_tag="IV228_000374"
     CDS             38712..40715
                     /locus_tag="IV228_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8716320.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            40734..41204
                     /locus_tag="IV228_000375"
     CDS             40734..41204
                     /locus_tag="IV228_000375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8716321.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            41225..41809
                     /locus_tag="IV228_000376"
     CDS             41225..41809
                     /locus_tag="IV228_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8716322.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            41824..42810
                     /locus_tag="IV228_000377"
     CDS             41824..42810
                     /locus_tag="IV228_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8716323.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            42800..43111
                     /locus_tag="IV228_000378"
     CDS             42800..43111
                     /locus_tag="IV228_000378"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8716324.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            43133..44914
                     /locus_tag="IV228_000379"
     CDS             43133..44914
                     /locus_tag="IV228_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8716325.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            44907..46283
                     /locus_tag="IV228_000380"
     CDS             44907..46283
                     /locus_tag="IV228_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8716326.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            46290..46925
                     /locus_tag="IV228_000381"
     CDS             46290..46925
                     /locus_tag="IV228_000381"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8716327.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            46955..48310
                     /locus_tag="IV228_000382"
     CDS             46955..48310
                     /locus_tag="IV228_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8716328.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(48502..49896)
                     /locus_tag="IV228_000383"
     CDS             complement(48502..49896)
                     /locus_tag="IV228_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8716329.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            49988..50959
                     /locus_tag="IV228_000384"
     CDS             49988..50959
                     /locus_tag="IV228_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8716330.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            50956..51738
                     /locus_tag="IV228_000385"
     CDS             50956..51738
                     /locus_tag="IV228_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8716331.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(51907..52278)
                     /locus_tag="IV228_000386"
     CDS             complement(51907..52278)
                     /locus_tag="IV228_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8716332.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            52412..52855
                     /locus_tag="IV228_000387"
     CDS             52412..52855
                     /locus_tag="IV228_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8716333.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(52898..53707)
                     /gene="yidA"
                     /locus_tag="IV228_000388"
     CDS             complement(52898..53707)
                     /gene="yidA"
                     /locus_tag="IV228_000388"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8716334.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(53726..55093)
                     /locus_tag="IV228_000389"
     CDS             complement(53726..55093)
                     /locus_tag="IV228_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8716335.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(55077..55907)
                     /locus_tag="IV228_000390"
     CDS             complement(55077..55907)
                     /locus_tag="IV228_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8716336.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            56070..56507
                     /locus_tag="IV228_000391"
     CDS             56070..56507
                     /locus_tag="IV228_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8716337.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            56583..57203
                     /locus_tag="IV228_000392"
     CDS             56583..57203
                     /locus_tag="IV228_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8716338.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            57392..57898
                     /locus_tag="IV228_000393"
     CDS             57392..57898
                     /locus_tag="IV228_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8716339.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            57915..58814
                     /locus_tag="IV228_000394"
     CDS             57915..58814
                     /locus_tag="IV228_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8716340.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            58901..59785
                     /locus_tag="IV228_000395"
     CDS             58901..59785
                     /locus_tag="IV228_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8716341.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(59977..60048)
                     /locus_tag="IV228_000396"
     tRNA            complement(59977..60048)
                     /locus_tag="IV228_000396"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60013..60015),aa:Arg,seq:ccg)
     gene            60108..61442
                     /gene="rpoN"
                     /locus_tag="IV228_000397"
     CDS             60108..61442
                     /gene="rpoN"
                     /locus_tag="IV228_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8716342.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            61645..62682
                     /locus_tag="IV228_000398"
     CDS             61645..62682
                     /locus_tag="IV228_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8716343.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            62723..63724
                     /gene="gap"
                     /locus_tag="IV228_000399"
     CDS             62723..63724
                     /gene="gap"
                     /locus_tag="IV228_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8716344.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYHTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            63834..65024
                     /locus_tag="IV228_000400"
     CDS             63834..65024
                     /locus_tag="IV228_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8716345.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            65128..65883
                     /locus_tag="IV228_000401"
     CDS             65128..65883
                     /locus_tag="IV228_000401"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8716346.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            65999..67297
                     /gene="eno"
                     /locus_tag="IV228_000402"
     CDS             65999..67297
                     /gene="eno"
                     /locus_tag="IV228_000402"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8716347.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        22297 a        11803 c        14131 g        19333 t
ORIGIN      
        1 aatagtaaca tttgcaaatg gagaacaact agagatggga aagagaagta ttgataaact
       61 aaagaatttt atgcaagaaa agatagaaaa tgaatcattt taaagaaaac aaagactcat
      121 atcttacctg ttagaagagt gttttcctgt agaagagaac aacccaagta gcagcctagt
      181 cgtttctatc cttttttgtt atgcactctt tttgacattt actccaccaa taacgaaggg
      241 tacaataaaa agtaataaca aacaaaagga gtacagaaat gattgaattc atcaatgtaa
      301 gtaaaagtta cgggaaaaaa caagcattaa aagacttgtc tctctcgata agacaagggg
      361 aaatttttgg atttttagga cataatggtg ccggaaaatc aactacgatt aaaagtttag
      421 ttagtattat tgaaccatca tcgggaacga ttttagtaga tggaatgaaa ttgacggaaa
      481 atcgattgag tatcaaacaa aaaatcggtt atgtgcctga ttccccagat atttttttac
      541 aattgacggc tggagaatat tgggacttga tttcagctgc ctatgaatta gacacacaaa
      601 aaaaagaaaa gcgtttagca gaattgacag cgctttttga tatgtatagt catcaaaatg
      661 aaacaatcgc aagtttttca catggaatgc gtcaaaaaac gattttgatc ggcacgttgc
      721 tgccagatcc agatatttgg gtattggatg aacctttgca aggacttgat cctcaagcag
      781 catttgattt gaaagaaatg atgaaggctc atgccgcaaa aggaaaaaca gtcatttttt
      841 ctacacatgt attggatacc gcacaacagt tatgcgataa actggctatt ttgaagaaag
      901 gtgaattgat ttatcagggg gcagttagtg aattattgaa tggctcacct gatgaaacgc
      961 tagaaaagat ttatttgaaa atggctggaa ggcaagcgac aggagatgga ccgtatgtat
     1021 aagcgacaat tattagaatt aattcgagtt aatcttttat atgttaatcc acagctcacg
     1081 aataaagcaa gagagaaagg aaaaaaggga aagcgattaa ctatctatct tttgtctcaa
     1141 aatctttttt cagggctgat ttttttactg atttacggat ttgctatgtt tgctatgaac
     1201 tttgttaaat tgccaggttt ctttacttat tacgtggcac tttttggtat gttaggttta
     1261 actcaaagta tatcagtcat ttataatatc ttttttgaag gaaatgacct gcaagtattt
     1321 ttgcctctgc cttttggaca agaacaggtt ttttcagcaa agattttggt agttgctttg
     1381 accatcattc cttttgtttt tccgcttcta gtcatgttta ttttaaccgg atggcgagct
     1441 gatctaggga ttttgcttac tatcattatc tcaatccttt tgttttctct atttttgatt
     1501 attctttttt gtctggctag tttgattgtt tttggtttgg ctcgaacagc agtgtttaaa
     1561 aaacatagaa aaacagtttc tagtatcttg cttggcagct cgatgctgat cgcatttgct
     1621 ggaatcatgt ggatgaatca tcaaacgggg actattgaaa atgagttaac agatcggcat
     1681 ccgatttcga tattattgcc attctatgat gctgccagtc agccactttc tcaacaaggt
     1741 ttattgggtt ggggaatact agctatcacg gctctgggat ttttccttgc tgtaaaacag
     1801 ctgatagtac caaagcttac agagcaactg actgattcaa acagtgagtt caaaataaaa
     1861 agaacccata aaaagaacca aaatgtgcat cagcttttat ttagttacaa tgctcaatta
     1921 gtaaaagaac ctaatttgat tatgcaagtt ctttcttctt ccttgttaac tccaatcatt
     1981 tttattcttg cttttgccct aggaggcgaa ataaaattaa ctgatttaga taataagtta
     2041 atcggtgttg ttttcctggt agggatcgca ttggctttct taacagttaa tcagacttct
     2101 tttatcagca atcttatttc tttggatcaa gaaaattttc tgtttatccg ttctttgcca
     2161 atctctatga accaatattt aaaagaaaag ttcagattcg gatggatatt gcagagtttt
     2221 ttaactggtg ggatcgcatt attaagcgga ctgtcttttc agctgccttt gttttttatc
     2281 cctagcttgc tatttggttc tttattcggc tgctacttat tgtcactacg ttatttcgct
     2341 agagattatc ggttgctgct gctgaactgg accaatatta accaattatt taacagaggt
     2401 tcaggaagtt tgggtttagt cgcaactatg atgggttcat tgatcatcag tattattctt
     2461 atcgtcttat acggaatgtt ggctttgttt tatccatttt ggttagtgaa tattcctgtg
     2521 atcggtttag ttctaatact ttccgctcta tggatcgttt attatcaacg aacgttttgg
     2581 aaaaaatttg aataaagaag aggggccgtg acaaaaatca tgtcgcagcc ccttttttac
     2641 agatagaggg tgttcaaaag tcagtttctc agtattaagt caattttatg taaaaatgtg
     2701 agaagctatt tttacaaaac tttttttaat actcataatt acttcttatt gctcctgcgg
     2761 ttacttgtcg caatgtttgt gagatatttt gtcatcgctc aaggctgaac gtctctttca
     2821 caaccttttc tttgttacac ttcgtttatt taaaaagccg tttttttcgt ttcatcatga
     2881 ttgcgtcacg ctttactttg aaattacgga tgttatggtt aaagacttct gataatacaa
     2941 gacctaaagc gattgctcca gcaatcataa ttgcttggac cgcataatga gccgcttctc
     3001 gataggattc agtaacgaga tttcgaaccg cttgataagc taaaccacca gggactagag
     3061 gaaccatccc gggaatattg aaaatcgtta cgggcatttt caaacgttta gagaaaagat
     3121 cgctgatgaa tgctactcct aaagaaccaa gcaatgaacc aaaagcagca gatccgccta
     3181 tttgaacaca gatccagtag atcatccagc caaaagcacc agaaagacca caacaaatca
     3241 atgaacgcct aggtacattt gtaataacgg caaatgccgc agtcgctaaa aaactaaaag
     3301 aaaactgaat aaatatcgtc cccatacttg tgttcctttc ctaataaaaa agctggaaaa
     3361 taaaagcaat cgcaaaaccg atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa
     3421 ctcctgagac ataatgtcca gctagcaaat cacggatagc gttcgtaatt tgaactccag
     3481 gaactaaagg catcacacat ccgataatga ttagatcctg attgcttcct aagccaaaac
     3541 gtacgctgta gatagcggct atgccaatca ataaagaaga taaaaattcc gataaaaatt
     3601 tgattctaaa aaatttcaaa ctatatagat aagctgcgta tcccaatcct ccaatgacta
     3661 aagtcacagg taaatcggtc atctttccgc caaataacac cataattgtt ccactgatca
     3721 ctgccgcgct aacgatttgc aaccagacag gaaagaaaga acggtctcgg tcgattgctt
     3781 gcagttcgca gtaaagttct tctaatgtta gctctgaagc aacatattta cgtgaaagat
     3841 catttacttt tactactttt tctaaattaa tcgaacggtt ggtaatttgt tccattcgga
     3901 tggtcgaggt acgatcaaga ccaataaaga gaccggtttg agtgacatag ctgaccaaac
     3961 ggaaatttcc agaagctaaa gcaattcgac tcatcgtatc ttctacccga tacatctccg
     4021 cattgctttc catcatgatt ttcccagcga gtaaacaagt gtccagcaac agatctaatt
     4081 cttttcccgt catagcatct tctccaataa ttgattgatt agttttattt tactacaaaa
     4141 cataccagaa gcgtaaagaa taaaaaaact tgaggaagga ataaaagagg cgtatactgt
     4201 ttttgtattg cgggtgtagt ttagtggtaa aatcccagct tcccaagctg atgtcgcggg
     4261 ttcgattccc gtcacccgct taaaataggt tgtgaaaaag ctgctctgca acaagtaata
     4321 agaacagcca agcaaaaaca acttcttatg tttttaactt ggatggactt attaccgcag
     4381 atgcaagctt ttgcgcaccg tttaccaagc ggttgtgaaa tcagtgtatt gtcttgagta
     4441 tgaagaaaat ttttgaataa taataaatac cttacagata gagtggtgac tgattagaga
     4501 cgaggaatcc gtctcttttt tgtattttta agccatctta aaacaggttt tattttttgc
     4561 tgtttctatt tctttctagt atttatttat aggggaacat atgttaagat ttagggagct
     4621 gttagtaaag gagtaaagaa atgagcaaaa aaacaaccaa ggcgatcgtg gggatcgtcg
     4681 ctgctgtgat tgcaggggca ttcggtgttt ccaccacaca aaaagattca gatatctttc
     4741 agcagattac aactattttt gattccacac cacaagcttc tcaagtagcg gatgtgccag
     4801 aatatgatgg aaaacatcaa gaaatagaaa tcaatgggaa tcagccggaa tttacaaaag
     4861 aggatcttag cctttccaaa ggaacatggg aaagctattc aaatattgat aaattgaaca
     4921 gagtaggaca agccaatgct atgcttggaa aagatttgtt ccctactgaa aagagagagc
     4981 ctttgtatat tgacccgaca ggttggaaac aaaagaaatt aagtgacggg caatggttat
     5041 ataatcgcag ccatttaatt ggtttccagc tgactggtca aaataacaac attaaaaact
     5101 tgatgacagg gacacgttcg ttgaatgctc catacatgct tgctcacgaa aatgatattg
     5161 cggcatatat caaagaaaca aatcatcatg tacgctaccg tgtcactcct cattttgagg
     5221 ggaatgaact ggtagccagg ggagtgcaat tggaagcaga aagcattgaa gacaaaaaaa
     5281 ttgaattcaa tgtctttatc tacaatgttc aagatggcta cacgatcaat tatgagacag
     5341 gacaagcaac aaaaacaaaa aattagtagc agctattcga attttttatg aatattatga
     5401 aaaaattgtg taaaaacata cattcaaaga aatatagcgt atactttatg aaaagagtac
     5461 ttttgataaa gctacagaaa aaaggaaaaa aacatacaaa tagtgtttct taatttattc
     5521 ggaagtttgg aatatttgtt tgtagttttc cctttcttcg agtaaaataa ggttgtagaa
     5581 ggtaagctgg aattaccttg cgggaaagag gtgtagaatc atgggtttta cagatgaaac
     5641 cgtacgtttt ggttttgacg atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt
     5701 ttatcgggct ttggaagaaa aaggctacaa tccaatcaat cagattgtcg gctacttgct
     5761 ttctggagac ccgtcctaca ttcctcgtta tcgtgatgct cgaaatttga ttcgccgtca
     5821 cgaacgcgat gagatcatgg aagtaattgt caaggactac ctctcaaatc atggagtgaa
     5881 tttatgagag taatgggctt ggatgtcggt tctaagacag taggtgttgc tatcagtgac
     5941 ccctttggct ggacagctca aggagtagag atcattcgga tcaatgaagc agaagaagag
     6001 tttggctttg atcggttgaa ggaattagta gcacaatatg aagtagaccg gtttgtagtt
     6061 ggattaccta aaaatatgaa caattccatc ggaccaaggg cagaagcttc catgtcatac
     6121 ggagaaaaga tcaaagaact attctccttg ccagttgatt atcaagatga acgattaacc
     6181 actgttcagg cagaacgtat gctagtagag caggcaaata cttcaagagc caaacgaaaa
     6241 aaagtcatcg ataaattagc agctgtgatg atcttacaaa attatttaga cggctcaggt
     6301 aagcaattat tttaataata aaagaaaaga ggtaatctta tgacagaaca taaccattcc
     6361 cacgaccatg aaggacacga acacatcaca ttggtggatg accaaggaaa tgaaacatta
     6421 tatgaaatcc tactaacagt tgacggacaa gaagaatttg gacgtaacta tgttctttta
     6481 tatcctgctg gtgtttcaga agatgaagat gtagaattac aagcttatgc atacatcgaa
     6541 aacgaagatg gaacagaagg cgagttagaa caaatcgaaa cagacgcaga atgggacatg
     6601 attgaagaag tattcaatac attcatggcg gaagaagaag aatagaacaa aagaatgaat
     6661 taataagata taaaaaaagg gattgtgaca aacgtcttgt cacaatccct ttttccaaat
     6721 aaacgatgtt caatcagctg cccctcggca ttcgctcgaa aaacggcgaa atatgaagaa
     6781 ctattttgcc atttgtttct ctaataatca ggtcaaaacg ttgacttcac agtctcttcc
     6841 ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     6901 aagctgtttt ttgaaattgt tccttgtgac tcggagctga acggcaggct cacgacctct
     6961 ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa gttcaaccaa acgaaaatta
     7021 tgaggagcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
     7081 ctcttttcgt ttagcactaa atttccttaa aaagtagaaa agctttcgaa gcctcgtttg
     7141 tttcttttac tgtataataa aagaaaagag taacaatcgc taaaagatat aaacaagaga
     7201 aaggaaatac aagatgattg gtctgattta ttttgtggtg atcgttctgg cgaacacagt
     7261 tggcgctatt tccgggatgg gtggaggcgt gttgatcaaa ccgattcttg atcttatcgg
     7321 cgcccattct gttgcaggga tttcttttta ctcaacagtt gcggtattca cgatgtccat
     7381 tgtttcgact gtgcgacaag tatcaagcgg gaaatcactg aattggcaga ttgtcggttg
     7441 ggtatctagt ggagctgttg ttggaggagt agtcggcaac attgtattcg aagttttttt
     7501 acagttattt gaaaacgaga agcatgtgca gctgatccaa atttttttga cggtattgac
     7561 cttggttttt gcgttcttct acaccaagca tcatcagcca aaatttcatt tgacgtcatg
     7621 gacttggtac cttatatgtg ggggggttct gggcttttta gctagttttt taggtattgg
     7681 tggtggacca gtcaatgttt ctttgttgat gctgatgttt gcattgccaa taaaagaagc
     7741 aacactatat tctctatcta cgattttctt ttctcaactg gcaaaattgg taacgattgc
     7801 tttaacaagt agctttatgc gctttgattt atctatgttg ttttatgtta tccccgctgc
     7861 tgttattgga ggtttgtggg gagcaagatt tagcagggtt ttgtctccaa aaaaagtgac
     7921 ctttattttt caagtaattg tcctcgtggt tcttttggtc aattgttaca atgcctttgt
     7981 catcttacaa acattaggag aatctagact aacgcaagtc aaaatcactg aaacatattg
     8041 ttctaaatga aaaaaggagt atactgtgat aaaaaaattc atttaaaaaa ataaaaattg
     8101 gctggtgatt tggatgttgc gggaaatgcg tttaggagat gcagaagcac tacgagaaat
     8161 caatgcggaa caattaggat atgatgtacc attaaccgtt acggtgagac aaatgaagaa
     8221 actgttgaag gagccagaaa aaaatttttt cttggtctat gaagatccta cttctaaaaa
     8281 agcagctggc tatgtacaag cggagtccta tgaatcgatt ttcagcgatc cgatgttcaa
     8341 tattatggca cttgctgtgt ctaaacatgc tgaaaaaaga ggaatcggaa aggccttgat
     8401 gactggcgta gaagaagcga aaagacgagg aatatcagca ataagactta attctgcaga
     8461 atacagagta gaagcacacc gattttatga acgcatcggt tatcattccg ataaaatgca
     8521 aaaaagattt ttgaaaatcc tgtaaatttt tatcaaaaaa tacctggtca tcaagccgat
     8581 acttgatgat caggtatttt ttttgactaa gaaagtaatt gcatcaagaa acctgagatg
     8641 atcaaaacga tggcaccacc aagccgattg cccatttgag caaaagcaat aagttccatc
     8701 cgattagctg cagataagac agctacattt cctgtaccac ccatcgagtt gttgcataag
     8761 ccagcagtaa tagttgattc gattggatat aaaccaaaca aacgaccgac aaatcctgaa
     8821 actactgaaa tcgcgataac acttgtcaaa cataagacaa caaattgcca tgtcaaagca
     8881 gaagccaata catttagatt caataacgca ataccgatac cggcaagaac agctgctgtc
     8941 aagtttttga caatcaagtt gttgaacatg atagctgcat cttcgtaata ttctggtaat
     9001 aggtttgcga ttttacagat aacaacgata atgatcatga atgcgtaagc atggacttta
     9061 ggaacaaaat agttacagat ttgtcctaag atgaagaaag tcaatgatac catcaaaccg
     9121 acacctaatt gtggaatgga aggttttaca tttctaggtt ttccatcgcc aaggtcgcct
     9181 ttttccatta atttaccatt accgttgaat tttggcattg attgtcccgc acgagcaact
     9241 aacgctgcac caatgatagc taggacattt gtcattgctg tcgcaggaat caggcgggaa
     9301 atgatagaag aagcgtccgt acctaaaaca tttgcataaa tcgttgataa aggtacagct
     9361 ccggctccaa caccaccagc cattgcgggc aatgagatat ataaaacgga atttgcaaaa
     9421 ccatttccga taagcatacc tacgagtcct acggcaaaaa aggagacgac cattgaaata
     9481 agtgcaacgg gaataaaacg aacagaagct ttcatcaata gattacggtt cataccaagg
     9541 atgctaccag taatcaaggc agcaatataa aagtctaaaa agcccatatt gctgacaaag
     9601 ttttctgtgg attttactac atactcaggt aagatcccaa atgtagcaat cgcggcagag
     9661 gcaaagatcg caaaaacgga gccaccacct aaataacttt tgacgatagg gatttttgtt
     9721 ccgataaaat gaaataaatt accgaaaatg accaaaacag cgattgggcc aatcatattc
     9781 gtcggtaatt ttcctaaagc cattgcaatg atcaggacaa ggaccatgat caaataaagg
     9841 ggcaaactta cgccggtaat ttttgttgac caaaaccctt cttttttctt ttcctcaact
     9901 gcagttgcgg ttgttgtttt ttgtgccatt tttacagatc ctttcttatt ctgtatcgta
     9961 tgaaaaaagc caaagagaag cggcaaaaga gcagtagttg gctggctgct gttttgccgc
    10021 agtcgctttt ttctttttag acgtcataca aagtttctgt atgttctttt actataacaa
    10081 tcttaatctt ctaaagattt atattcagga acccatttag cagattcaac agcttctttg
    10141 atatccgtga tttcttcacg attcaatttt tgtgcaacaa ctgattttgc gacagtttca
    10201 gcaatcgttt gagagaattc agtgattttt gctactggag gcaagatagc tgctcctggt
    10261 tttgttacat caacgattcc gcctaatgct cgactagctt gagaaatgat ttcagcattt
    10321 actcgtgtag ctgttgaagc aatcaaacca agccctaaac ctgggtacat caacgcatta
    10381 tttgcttgac cgatttggta agttacaccg ttatattcca catcagcagc agggatacct
    10441 gttccaacta aagcttttcc gtccgtccat tcaatcaaat cttttgcttt tgcttctgcc
    10501 aattttgttg gattagacaa aggcatgatg attggacgtg gggtatgagc cgccatttct
    10561 ttcacgattt cttcagtgaa tgctccaggt tgggtagaag taccgatcat gatcgttgga
    10621 tggattgctt ttacaactgc ttctaagttt gtcaattcat cagcattagt gaattctgag
    10681 cgtttacgag cgaatgggat ttgaccagga gtcaatcctt ttgtatcttc aaacaataaa
    10741 ccctgtttat ctacttgata gaaatgttgg cgtgcttctt cttcaggtac accttgacgg
    10801 atcatttcat ctaaaaggat gtttgcgata cctacaccag cagtaccagc accaaatgtc
    10861 aagatcgttt gatctttcaa ttgctcacct gatatattca atccacctaa tacaccagct
    10921 agtacaacga ttcctgtgcc ttggatatca tcattgaaag tagtgatttt atcttcgtat
    10981 ttttctaaga tcgttgcagc atttcctcgt ccaaaatctt cccaatgtaa taacaattct
    11041 gggaataatt cagtagctga ttgaacgaat tgatcaatga attcgtagta agtttcgcct
    11101 tctacacgag catgacgatt ccctaagtat aatggattat tcaataattc ttcgttatta
    11161 gtaccagcgt cgattgatac aggcaagact tgtgcaggat tgataccagc cgcagcagtg
    11221 tagaccatca atttaccgat agcgatgtca acaccgttca caccccagtc acccatacct
    11281 aagattcctt ctgcatctgt cacaacgatc aaacgaatgt cgcgtccatc tgctgcgttt
    11341 ttcaaacttg ctttgatatt ttctggttcg tcgattgaaa gaaatgcagc atcttgtggt
    11401 ttcaagaaaa tttcgttgta ttgttcgatt gaatccgcaa cgacaggatc ataaacgaca
    11461 ggcatgaatt caactaaatg ttcgcccatc aatttataaa acaatgtccg gttcgtattg
    11521 aatagattca ttaagaagat acgtttttcc aaatcggtct gtttgcttaa gtattgaccg
    11581 taagcttgga cagattgttg ttccaatgtt tggacagtac ttggaagcat accagtgatt
    11641 ccgtattttg cccgttcttc ttttgtaaaa gctgttcctt tatttagaaa aggattattt
    11701 aataagttta gccctgtaag catgaatgat tcctcctaag ttttaataca aacgaagtat
    11761 aggagtcaga tgaggatata gtcaaaaaga gcaggtataa taaattttat ttatatgagt
    11821 aatagagtca aaagaaatca aaatagcggt atttgttctt tttatatcaa caatccttgc
    11881 ttgattacta tgcgtatctc ttatatgcta aagaaagaaa agtagaagaa taaaggatat
    11941 gttagataga taaatagaat ctctgaagaa taaatcacgt gatcgtgacg ataaaataca
    12001 cttacgtcag gtgataaaaa atacataagt agaaaactaa gaggaggaac agaaaatgaa
    12061 agaagttcaa ttgatcagaa aaagcgaact atcagaaggt ggttgcaatg catgcggcgt
    12121 agtagaagca acaagctata cattaaagct tggtgccaac aaagcgataa tctctgagtt
    12181 gacagtaggc ggtcttgtgg acagtctggc gcttgctgaa ggttttattg gagaagatat
    12241 ttatgagatg ttttcagaaa tccgtcagtt aaaaaaaggg gaaaactgca tagaggttca
    12301 tcatgagagc ccgaatgtga gattcaaacg tggtgataat gagatgatct tcaataatca
    12361 tgtgtccgat cacaccgagc tatatggaat cgtgaatcaa atactaactg agctttttgg
    12421 attgggacct tatgcgttca aggaagaaaa cggtaatcca aaattaaacg aagaatggca
    12481 agaaacgatt gaaatacaaa gaaacaaccc gcacttattt caataggcat ccacagtatt
    12541 tcgtaaaagg gactttgaca cgacttttgt caaagtccca tcgtttttta tctatttgtg
    12601 ttctaaacat ttctttcaga gtagtaacaa ctggaatgac tgaaatgtaa acagattatt
    12661 tgcctgtatc aatacggaag agtaatataa agtgtgtaaa cgctatcata ttattcattc
    12721 aattttttat agagggaagg cattgaaatg gaaaaaacgt atcaagcagg tacaaatgaa
    12781 ggaatagtgg attttatcaa tatggaagat ttagaaatag ccgcttcaca agtgattcca
    12841 gctggtggtt atggttacat cagcagtggt gctggcgact tatttactta tcaagaaaat
    12901 gaacgggcat tcaatcatca gctgattatt cctcatgtat taaaagatgt agaattaccc
    12961 gatacaacta cacattttga caaagaaacg ctgactgcac caatcatcat ggcaccagtt
    13021 gctgctcatg gcttagctca tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat
    13081 tttgggacga tctatacagc aagttcttac gcttcttgta cattagaaga gataagagaa
    13141 gcaggcgggg agaaagcacc gcaatggttt caattttata tgagcaaaga tgatgggatc
    13201 aatttggata ttcttgaagt ggccaagcgt aatggagcaa aagcaatcgt attgacagcg
    13261 gatgcaacag ttggcggaaa ccgtgaaaca gaccgcagaa atgggtttac cttcccactg
    13321 ccaatgccga tcgttcaggc ttatcaatca ggagtaggcc aaacgatgga tgctgtgtac
    13381 aaatcttcca aacagaagct tagtccaaaa gatgtagaat ttattgctgc tcattctgat
    13441 ttgccagtct atgtgaaagg tgtccagtca gaagaagatg tctatcgttc attagaatct
    13501 ggtgctggcg gaatctgggt atctaatcat ggtggccgtc aactagatgg tggtccagca
    13561 gcgtttgatt cgttgcaata tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt
    13621 gacagcggtg tacgacgcgg acagcatgtt ttcaaagcaa tcgcatcagg tgcagatctt
    13681 gttgctattg gcagacccgt tatttatgga ttgtctttag gaggaagtac aggagtccat
    13741 caagtgtttg atttctttaa aacagaattg gaaatggtca tgcaattagc tggaacacaa
    13801 acagtagaag atatcaaaaa aataaaattg cgtgagaatc gttttattta agaaacaaat
    13861 gacgaaaaaa ataggagtgc ggcagatgat gccgtactcc tatttttata gttcaaggat
    13921 gtcactaata gaataatttg aaacgaaaaa ataaaattga aatatttttt catacttaat
    13981 aaaaaaggaa gaatattcat gtaaacgatt ttattgtttt tttctctttt atacaaaaag
    14041 ggtcataatt tcaagtaagc ggatgaattc atgaggattt tttgcatact gtctctacta
    14101 tacttaggtc actaagaaaa aggagtgatt tattatgaaa aagaacatga ttgggacatt
    14161 attactttca acacttcttc taggcggctt ggcaacacca gcttttgctg agggtcaagc
    14221 gacttcaaaa ggagatatta cctttactga accaacaaat accgtggagc ctcttaaccc
    14281 tactgatcct tcaaaaccag tagagccagc agatccggaa aatccagcaa ctggccagac
    14341 aggtccttta acactggatg tagttcccga attgccattt ggtacacacg agattgaaag
    14401 cggaacaaaa acgtatcaag tagacgcttc aaaaaacgat acaccttatc tccaagtatc
    14461 agatcgtcga ggtgtaggtg cagatggcca agcacaaggg tggaacgtga cagtttcggt
    14521 ttcagatttt gtaaacggtt cacaagtttt gcaaggcgct gaattagatt ttggaacatc
    14581 cactgttaaa agtacgtcag ataacgaatc cactggacca actagtcaaa ctgtaactgg
    14641 gttgagtaaa gcaagtgcag cgacaccaat atttacagca gcaaaagatc aaggtctggg
    14701 aacatggctt tctgtttatg atccagcaaa tatcacactg aaagtgccga aagcagcagc
    14761 tggaacattc acggctgatt tgacttggaa cctcgtggct ggcccagtag catagagtat
    14821 gaaataaatt caacaaactg cggtgaaaat actataggtg aaggattttt taccgcagtt
    14881 tttgttgaaa ggatgtggtg gacaatgaat caaagtaaac gactgtttgt ttcgtttttt
    14941 tctattttaa gtctattttt catttttccg tcaatcagcc aagcaaagga ttcagcagga
    15001 gactttggaa tcaaaccggt atttccagag aaccaaatag ataaagcaat cggttatttt
    15061 gatttattag tggcaccgga acaaaatcag acactagaag taattatcag caattcttca
    15121 gatgaagaac gaacatttga ggtctctgtc aatccggcgg taacgagtga tggtggaacg
    15181 atcgattata gtcaaaaaaa cccaacgtta gatgagacat tgccgtttga tgtccgtgac
    15241 gttttgctca ttgcgaaaaa ggaaatcaat gtatcagcac atgcagaaac aactgtacct
    15301 atagaagtga aaatcccagc aaaaagcttt aagggacgtg tattagcggg tattcatgtt
    15361 tcacctaaag aagaagctga aacagaaaat gctacagaag gagcacaaat caaaaatcgt
    15421 attgcttata atctcgcagt cgttttacaa gaaagtcaag agacgataga accggatctg
    15481 aaacttttat caggagattt ggacgaggtc aacgcaaagc caactgttca gcttcgtttc
    15541 caaaatcctc agcctaggat tatcagcaat ttgatattta cgagcaagat cttttatgaa
    15601 aatcagcttt atattgaaaa tacgtctaat gcatttttag ttgcaccaaa ttcgaatttt
    15661 cacttgaatc ttgatttagc tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc
    15721 atagcaaaat caggcgacag taatgaatgg cgatttacac aaaactttac gatcaaaaaa
    15781 gaaaaagcac aaaaagtaaa tgaaaattcc gtttttgcag tacaagaaca atctttccca
    15841 tggctttatg caggattggt tgctgcagtt ctagtgttat ggttgatttt gctgtttttg
    15901 tgggtccaca agaaaaagaa aaaaggagaa acaacatgat gaggtgattt cgttgaaaac
    15961 atcgatctta ttgggaggga ttattgcaag tttgctgttc ttcagctggc aagagccagt
    16021 ccaagcagat accaaaagtt caggtacgat tacattttat gatctaagtc caattccgca
    16081 tccaagtggt tcagtcaatg gcgcatcaaa acagcagaca cagctaccga tttataaaga
    16141 atctaatcaa catggcgtat tcccggagac gaatgaacaa aagatccgtt ttctttattt
    16201 gatcattggt atagaattat ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa
    16261 ggaggatgaa catgttgcta caaaataaat tttcattttt tcgctccttc ttttttactt
    16321 cgctttttgt tttaagtata tttatctttt caccttcggc agaagctgtt ggagcaacgc
    16381 ctcctgccat ttcttatgat ccatacacgt atgaaaatat cgtacctcca aatggtggac
    16441 gtagtgcagt caatggcggg aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat
    16501 tgactgctac ttatttgaac gatggttcaa atgatccttg gaaaggtggc acggtccgtt
    16561 ttcattggga aagccatgtg aaactaacaa atgtacttga tttggtgaat atcactgcac
    16621 aaggatttgc atttgtggcg atcatcaatt tgccggaaca tgtatcaagc agtgatgtat
    16681 taaaagcaat tgactggaac aacgcctact tgtatattgc cggtgccccc atcaaggtta
    16741 ctagcggaag tttattatca gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg
    16801 atacaaatac tctattgaca gatattattg ctattattac gcgaggcgaa ttcaacgtgg
    16861 acgatatccc gcttcaattt gatgttacag ctaatgtcgc gaagatgaca gaaaacggag
    16921 ataaatatga tacgagtcca aacaaaattt tgaccaaggg taaattacaa ccttctatgt
    16981 ctaaaaaggc cgttttttct gttgattttt atgattcgaa taatattatc caagatcgta
    17041 tgttcaattc gattttagct ccagctgcag gtagatttta tcctaaatta actggcggaa
    17101 acttgaatcc aggagcatat gcattttctg caagtgcaga gataaatacg tggaatagtt
    17161 acatctcacc aagtgattca acaggaaaat ataatacatt tgctgatacc acagaatacg
    17221 tggatggaaa taaccagaac cttccaggct ctaccaatag tcggacattg acgatggggc
    17281 tgactgaagg acgttttgat cagttgccat ttagtcgctt tgatcgcgta gtcaactatt
    17341 ttacaggaaa aaacgttaca caaggagcaa tactgactca tactccagca agcacaagtg
    17401 gattgaatca gacagccaat atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt
    17461 ctcctgttgc tttaaaggta ttggagcagt acaagctaga cggaacggtt ttaccaacaa
    17521 acttaacagc cgatagtcag tggggaaagc tcctttcttc tgcatcgtac caagtcacgg
    17581 ccaattggtc agcaactgaa ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag
    17641 gaacccaaaa attagcgaca ggatggacag atcaactttt ccaaacgatc caatcaaata
    17701 acgggggtgt aaatacagct acttctacac tacctgcatt ggcttcaggg caatattatt
    17761 ttgattatcg cttggtggac gatgtactta ctaacatgta tccgtctttt gcgtacaaat
    17821 ggcagtctga acaagctgga atcaccactc ttcctcaaat aacagtggct aattttccat
    17881 ctgtgagtgc agatagtcag ctaaaaaacc ttagcagaca acctgagaca ggagatcccc
    17941 tgtctgctct atcaggagat acgattcaag aaaatgtgac attcacgtta acaaaatctg
    18001 gggatgcgat aaccgacaaa aaaatcacga tctcacttcc tgaaaatact acttatatca
    18061 atggttcctt aaaattaaat ggtacaacta tcgcagatac cggcattcaa caaggagttt
    18121 ctgtcccagc agatctattg agcaaaatag gagataccat tcatcttact tacaactatc
    18181 aattaaatac tgtagatact tctttacaat ccgtcagcat actgacaaag gcagctgtac
    18241 tttccagcaa tattacatta gcagatggtg ctaagcttcc taacccagtt gtccaaacat
    18301 ctgctaaaac aattcttgtc cccaaacaag aattaacatt agttaatgta ccagatgatt
    18361 ttacatttgg taacgatctc cccaaaccgt tgaagacaag ttattatgaa tcaaaaggcg
    18421 atttttcttt tgatgtgcgg gatactcggc ttccaagcac gtctccttgg caactgacag
    18481 gtacattgac ttctttattt aagaatgatc aaggtcaaga attaagcgga acaaaattat
    18541 acttcaatca ttctggatca aagcaattga ttcagcaagg tcaaaacacg ttgatttatg
    18601 agagtgatgg aacagctaaa ggagaggttc tcgttgactt tcccgatacc gatggtttac
    18661 tcttagaggt aaatagcagt acgaatgctc aacctggcgc aacgtatcaa ggcatggtca
    18721 catggaaact gacagctggg ccaaccagct aagtgattag aatttgtatg gatctgtgat
    18781 aactgtcaca gatcctcttt ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa
    18841 atgcttgaag gagtggactt ggaacttttc agtttgcttg aaaaaaatga acaacggcaa
    18901 atcaaacttt tccaactttt attagaggaa aaaggagagg tttccgttaa taacgtactt
    18961 acccgcttat cagttgatcg ggctaccttg aaagaagatc tgttttattt acaagctaac
    19021 ttggaaaata tcaaacaatt ggaaattgtt atccaagaag ggaatatttt attaatcaga
    19081 catggaaata tagcagcagt cgatgtttat tatcattgtt acttgcgcta ttctacaaaa
    19141 tatcagatac ttctttatct gttggaaaat ggctcatttg atcggcagaa acttgtcatg
    19201 gaacttaata tcagttcggc tactttgtct cgcagagtga aggaattaaa tgattcttta
    19261 aatgagtttc atctgaaaat aaaaagcggg cggctgaccg gtccagaaag tcaaattcgt
    19321 tatttttatt tccaattatt ttggtttgga cgtccctatc ttgaaaacaa aaaagaatac
    19381 agtgatttat caggaaacac ttttttagat ctgctgcaaa aagagtttag ttttgctatt
    19441 acagaagaag gacgattgaa atttgaaata tggctttcca ttatgaagaa gcgaatgcgc
    19501 tgtacgaaaa aatccaaacc agattatagc aatcttcctg ttggattgtt tcaaacgaca
    19561 caatttttat tggacctcca tacatttttg acccgttact tctttcaata tgcctttcct
    19621 tggaatcaaa atgaaacgtt ggttttttat cttttcatga tcagtaactt tacattagat
    19681 acacataact cgcatgtcca ggagtttttg ataagcgaaa aatacaaaga cgacgtagta
    19741 gctcaattga atcgaacgtt taaacaatcg atagaagaat atttctcttt ttatgttttt
    19801 ccagaagaga tttcgcaaaa gattgcgttg acagtgatgc aaaatcacgc tcattttgcc
    19861 tattttagag gatggatgaa tatttttggc caagaaggat taaaacagcg gttaactgaa
    19921 gtagaagata aacaattcct ggttttatgt gaaagattgg tcagacaaac atttgctatt
    19981 gttgatttta ctgaagtaga cgaggaaaat gcccgctttg aattatttgc tcgctatgca
    20041 tctattttac ggacgctgat caagcagtta gaattacgtc tgcaaattgc ttatgatctt
    20101 ccttatgaaa ggctaatggc ggatatcgtg aaagaaatca tccaggaaca attgctgaat
    20161 acgatcaaat atgatttagt cgaatacgag aaagaaaagc agtatgatat cattttgact
    20221 agccaattga aagagtaccc gtcacagaag tccgctaaag tttttgtatt cactagcaac
    20281 gaatataatt atgatttccc ctatttgaac caatttttaa aagaatgtta tttagaaaaa
    20341 ttgaatttaa ggatgaataa aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca
    20401 tcacagctgc cctaaatcta agaaaacttc aaccgaaagc agaagtttta ctgatcgatg
    20461 aagatcataa agatggacta ggctatgttt caaatggaat caacctttat ttaaaaggaa
    20521 agatacgtag tttagctgaa gttgcccata acatgcggac tcttcaagaa agtggggcaa
    20581 aactgatcac cgaatggaga gtaactgaac ttgatccaga caaacatcag ctcatcctcg
    20641 catccaaaga aggaaaaaac gagaccataa cctatgataa attaatcgta gcgacaggtt
    20701 cctctcctgt caccttgtat aaacaaattg aagctgaaaa tgtctacacc tataaaaatc
    20761 tagtgcaatc taagcaagta cttgccgctt taaaagaagc aaaagaagta gtgatcttcg
    20821 gtgctggtta cattggattg gaattagcag atgctttgag aaataaagga tacattattc
    20881 atctagttga ttatatgccg aatgttttat caagatattt cgataaggat atgatcaaca
    20941 gttttcaaaa tcagctgcaa actaagcaaa taaattttta tccaaatgaa ttcttgatcg
    21001 attggaaaaa atcagaagag aaagtagtat ccgtacaact tctgtctcaa acaataaagg
    21061 cagatatggt cattttttct gcccaaactc gtcctaatac gacattgtta aaagaaaaag
    21121 tggctttgta tgaggatgag actgtgatag tcaatgaata tctgcagaca agtgatccgg
    21181 acatctatgc gattggagat atcgtcccag tcagttttga caaaaataaa cggcatctct
    21241 ttttaccttt agttactcga gctgtacata tggcaagagc agtagcgcta actttgagtg
    21301 gtcagccaac cgcttacgac ctcagacaaa agataacagc agcagtcatt atagactact
    21361 ttttaggaac tgtagggctg acagaagatg aagcgccatt cttggaacaa aataccggtt
    21421 cgtgttcagg agaatttgat ctatttccac aatacgacga agaaaacaaa acagtaaatg
    21481 caaagctgat ctaccatccg gatacattag aaataattgg cggacagctg ataagccaag
    21541 aattcttatt atcagatttg aacctgcttg cggatatcgt gaaacacaaa actacgattc
    21601 cacagctagc agttgaggat tttggatttc tgtctgaata cacctctcgt tttcactacc
    21661 tcaatgagtt agcttttaaa gtactagcta agagagcaaa tagcaataga gtagataaaa
    21721 gactgtgaga aatatcacgg tcttttattg tgtggggata taataaatat taatcaatcg
    21781 agaataaaaa attcatataa tgaaatagcg gaagaatttt ttgagcataa tattcgccaa
    21841 aaatttatca agtgataaaa ttgttgtata aaatgattat ttcaggagga ggagttaaat
    21901 gtttttagca ttaaatgaaa ttatgcattc aaagttacgt tatgcgttag ttgcaggggt
    21961 gatgtttttg atcgcctacc tggtattttt tttgactggg ttagcttatg gcttagctca
    22021 agataatcga acagcagtcg acaagtggga ggcagattcg attgtgcttt cgaaagatgc
    22081 aaattcaaat ctagggatgt ccatgattac taagaagata gcagaagaag tcgaaggtgg
    22141 gaaagtggcg tatcttgccc aaacacctgg agtcgtgact agtaaagaca gtacagaaga
    22201 aggaaaaatc aatgttagct tcttcggtat cgataaaaat caattcatca tgcccaatct
    22261 tgttgaagga aaagcttttg ataacgatga tgaggcagta ggcgatatta gtttgaaaga
    22321 agaatatggt ctagccatag gagatacagt taagttatct ggtagtgaca agacgtttaa
    22381 actaactggc tttacggatc atgccaaatt caatgtctca ccagttctct atacaaccat
    22441 caatgcttac caagaaatcc gcttcgaaaa agaagataca agtgaaaatg cacggattaa
    22501 cgcaatagtt gtcagaggta aaatcagtaa tttacctgaa gatttagaac agatcaaaat
    22561 ttctaaattc atcaatgaat tacctggata taatgcgcaa gtattgactt ttggatttat
    22621 gatcggattt ttgatcgtta ttgcggctat tgtgatcggg attttcattt atgtactgac
    22681 gatgcaaaag attaatatct ttggtgtaat gaaagcacaa gggatcacag gaggatttat
    22741 tgcgcgttct gtagtggcac aaacattcat tttatcattt gttggtattc ttcttggtct
    22801 attaggaaca gtgggaacat cccttgttct acctgatgcg gttccattcc aaagcaattg
    22861 gctgtttttt ggcgtcatta gcttgttgat gttagtagta gctgtgttag gcgcactatt
    22921 ttctgttcgg acaatcgtaa aaatcgatcc gttgaaggca attggataag aaggaggaag
    22981 aacatgaaag caatagaatt taaagaagta gaaaaaagtt tccaagatgg cgaccaagta
    23041 gttaaagcat tgaaagaaac aaccatgagt atcgataaag gggaatttgt tgcaatcatc
    23101 ggtccttctg gttcaggtaa aagtactttt ttgactttgg caggcggatt gcagacgcct
    23161 agcagtggct ctgtatttat caatgatcaa gaattcagta atcaaaaaga aagcgaacgc
    23221 gttaaactac gttttaaaga aattggcttt attttgcaag cttccaatct ggttcctttt
    23281 ctgacagtaa aaaagcagtt attattagta gataagatta aacatgaaca tcgtcaaaaa
    23341 gaagcaaacg aattatttga gcagcttggc gtggataagt tgatcaacaa gtatccagaa
    23401 gaactatctg gtggagaacg gcaaagggtc gcaatcgctc gtgcattgta caatgatccg
    23461 tccattattc tagcagatga accaactgct agtttggatt cagaaaaggc acgagaagta
    23521 gtcgatatct tggcaaaaga atcaaaagac aaaaataaag caacgatcat ggttactcac
    23581 gatacacggc tgattgacaa atgcgataga atatttatga tggaagatgg tgtattgaaa
    23641 gaaaaagaat aaccaaaaag caggtggaaa aatggtccat cgatacaaca aagatacaca
    23701 gcaaaaaatt ttgaaagaag cgactgactt gtttatgtcc aagggatatc tcggcacttc
    23761 aacaagagaa atcgcacaaa aagttgggat cacccagccc aatctctatc attatttcgg
    23821 tgataaagaa aaactatata cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc
    23881 tttgagagaa atcgttcaaa caagcgaaac gggatttcaa gtaaccttga caaaaatggc
    23941 gcagtttttg attgagacac atctggtcga tttgtttctg atgcttcacg atcttgaaag
    24001 caatctttca aaggaaacaa gagatcatct tttctttcta tggaagaaaa attatcgtga
    24061 accttttgaa gaaatctttt cagcaaacca atctgtgcta agaaatggga tttcaaaaga
    24121 aatcgcagca aggcattttt tcttagtgtt agcaccttac atcactcggt cgaaaagacc
    24181 tgtggaagaa ccgctaaatg tggcgcaatt gatcgattta tatttgaatg gtgtattagg
    24241 agaagcgtaa agctgttata tagggagaaa atagttaggt gaagaagcaa gatatgacga
    24301 agatcgttat atcttgcttc tttatatagg tagtttttca taaagacaaa tttgttattc
    24361 tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga agtgttttaa
    24421 tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt gggtagatga
    24481 atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa aaatcatttt
    24541 agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc ttacttaagc
    24601 tgattttaag ttttggccct tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa
    24661 taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa atagatgttt
    24721 ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg gttacaaaga
    24781 caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga aaaaatgacc
    24841 gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac ggcggagagc
    24901 tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat gatggctata
    24961 ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa acaaaaccgg
    25021 aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat gatactgggg
    25081 cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca gccataaaag
    25141 taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact acagatgaat
    25201 tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat gtctttatca
    25261 gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg acatattatt
    25321 actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact gatctgcctc
    25381 tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat cttcaacctt
    25441 ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa ttagtaacag
    25501 acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact tattttcagt
    25561 aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg ccgggcttat
    25621 tatcgcagat gcaagctttt gaacaccgtt tattttttta gtagcttcaa agctttttct
    25681 gtatttgcaa agtgaagttt ggcatatttt tcaagcatag gcatgccgcc tttctccaat
    25741 agttttgccg ctacttggtc agatttcgtt ccagctcctg atggaagggt gattccagct
    25801 tcagctgatg ctttgtgtaa ctcttccaaa aagctggcac gactgatgat agaagcagca
    25861 gcaacagcta aatgatattg ttctccttta gtaacgaagt acagtttttc ttgtaattga
    25921 tttttttcta aacgcgcata ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa
    25981 atcgcttctg gtttttctgg ctcgattttt tttagcagca aatggatcgc ttgattgtgc
    26041 aaagcgactt tcatccgaac ggcattgtat tttggttgga tctcattgta tttttctgga
    26101 gataagacaa gcagtttata ggggatggtt ttttttaaga tgtcagcaag ctggatgatt
    26161 tggctatctt ttaactcttt tgaatctcta acaccaagtt tacgtaattc tgcaagctga
    26221 tcacgtttca cataagcagc acatactgtt agcggaccga aatagctgcc atttccgacc
    26281 tcatcactac ctaaaacgga taattgactg atattttttg gcaaagaaga ggctgttttt
    26341 gctggcttgg cttgtttatc ctgtgcctcc ccccatttat cagcttctat tgctgcattc
    26401 gtaccttgaa acatcacttt tccggatgta taagcggtaa tggtcgtccc gtcttttttt
    26461 gcagcaaagc tagtataagg aatctgttta tttaacaaat aagagcgata gtatgctttc
    26521 atttgactaa gttggtcgtt tgttgctttg ataatgacgt tgctcataaa attctcctcg
    26581 tttttataca taaaactata ccgcaactca cgacttttcg ttttctaaga gattgcgaga
    26641 catagcattt ttttaaagtt tatcatataa tagttaagta tgtgaaaaat atttgtggag
    26701 aacggtgaaa gaaccaactt catggtacac taaataaaac tttcatcaaa taggaggaaa
    26761 tgattcgtat ggcgcacgaa aaaacacgct acaaagcggt gattgccaac caaacatata
    26821 ctattatcgg acgggaaacc aagcatcata tggatatcgt aacgaagctg atcaatgaac
    26881 agttagcaga attgaaacaa ttatcaccac aaatggataa tgaacaagca gcaatcttga
    26941 tggctgtcaa tgcgctttcg gatcaattga agaaacaaga aagaatcttg gagctggaag
    27001 aagaaacagc agaattaaag aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga
    27061 aacgcattga agcaatcgag aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag
    27121 atcataccat taaaaatcat gtggaagcac aacagatttt aaacgagaaa cgaaaaggac
    27181 aaattaaaca aaaatcttct aattaagatc cttttatcaa aataagaaaa atttaaaaaa
    27241 agttagaaga gaaaggaacg tagcatgctg agtttgctga ttttattttt attgctaatt
    27301 gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa tcatattcgc gattggatat
    27361 gtattgtcat ttattgctgc ccagcatttt tacaaaccat tagcaagtca tttggaatta
    27421 tacattccat acccagctgt cacgccaaca tcaaagttag cattttttga tcaggtattt
    27481 gcctttcacc tagatgaagc tttttatgca ggaactgctt ttctgattat tttgctaatt
    27541 ggctggctgc ttacccgatt tgtgggggtt tttgtccatg gattgacgta tgtgccgatc
    27601 ttgagacagg tagactggat tgcaggaggc atcctgtcac taatcatggc ttacgttact
    27661 atatttttga ttttgcagtt acttgccttt gttccattag atgtggtcca aaatcaattc
    27721 ggcgagcata gtttcgctcg ctttatcgtt gagagaacgc catttcttac caacaagatg
    27781 tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa aatatttcgt
    27841 tttttgtaca gagtagatta tttaagaagg ctgcgtagcg tatgtccggc acagctcttt
    27901 tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac attggaattt
    27961 gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa agaagaactg
    28021 gcagaattgg taccagtgag cgaggaacaa acgatcacaa actggcttca agaaactgag
    28081 gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaact tgaaaatatc
    28141 cgtccccata tgaaacggat tgagatcgga gcagacttga atggagtgga gcttgcacaa
    28201 gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga tctttatgac
    28261 agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac gatccctgtt
    28321 ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga tgacgcttct
    28381 cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt tcgtgaacag
    28441 ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat catcaccatg
    28501 cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt tggcggtgta
    28561 gtccacgatc aaagtgcttc aggacagact ttatttattg aaccaaaaca agtggtggat
    28621 ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca acgtatttta
    28681 agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa tgcttatgtg
    28741 attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt aaaagctatc
    28801 gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca tccactgatc
    28861 gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca agccatcgtc
    28921 attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg tctgttacaa
    28981 ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat cggtattttt
    29041 gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct atcgaccttt
    29101 tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa aagtctggta
    29161 ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct agcaatcgca
    29221 atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca ttatcctgaa
    29281 ttgaaagttt atggatataa tcgggcaaat acgattaatg ccagcatgga gtttgatgtg
    29341 gatacattaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag caatgcgttt
    29401 gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa gcagttgatg
    29461 aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg ccggaaaatg
    29521 gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga acttcacgat
    29581 gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat ggaaaaagca
    29641 aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa aagcagaaaa aatcattgct
    29701 gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca tcagttgatc
    29761 gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa aaataaagta
    29821 ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt ggttacgact
    29881 tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt agaaatcggt
    29941 atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca aaaagagccg
    30001 acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc gaatcaattg
    30061 gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata tttggattct
    30121 gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac aggcgcactt
    30181 cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt tgagtttgca
    30241 ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag cttactaaag
    30301 gtaagcagat ggttcgctat ccaagtatag aaatgctata ctagtcatag attttaaaaa
    30361 tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa gaaacagata
    30421 aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt atgcaagcac
    30481 cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt gctaaaatgg
    30541 atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt ccaacattaa
    30601 tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca aaagaacaac
    30661 tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc tgagggtggg
    30721 actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt caaattaata
    30781 ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca tgctataata
    30841 actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa caaattagct
    30901 ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac gatcatttat
    30961 gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg tagccataat
    31021 acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt tacagaatcg
    31081 aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc taaatacaac
    31141 attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga aaaatatccc
    31201 cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac tgtattttgg accatatccc
    31261 gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc tctgagaaaa
    31321 tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca atgtctgtgt
    31381 ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga agtgaaaaac
    31441 tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat ggaaaaagct
    31501 gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg tgccattgag
    31561 acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga tgtatttggt
    31621 tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt tcgtaagaca aggaaagcta
    31681 attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga tttcttgact
    31741 tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt cttgattcct
    31801 gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct gcaaccaaaa
    31861 cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga atgcctcagt tgctttgaat
    31921 gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt tgaacgttta
    31981 ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa ctctaatatc
    32041 atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc ggcaagaaaa
    32101 gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc atcaatgaga
    32161 gaagtcatct atcgcagata ttcgcgagtg attcgcgaag ggttgccttt acctgatttg
    32221 atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt agaaaatcaa
    32281 ttaggaatcg atattcctat tgctggcatg gcaaaaaacg ataaacataa aacaaatgag
    32341 ttgctattcg gtacagattt ggctgtagta ccgcttgagc gaaattcacc agagtttttc
    32401 ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca tcggcagcta
    32461 cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aagggttagg accaaaacga
    32521 aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc cacgacagaa
    32581 gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga taaattacat
    32641 caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt ttatgtacaa
    32701 caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg gtgtctaagg
    32761 atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga tcttgttaaa
    32821 acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga tggaaaaatc
    32881 atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt gatcttggat
    32941 tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg ggagaaagat
    33001 tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt aacgattcaa
    33061 gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat tgaaccaaaa
    33121 atcgactttt ggatggaaaa atttcaggta aaagggaaaa agacggataa agtaaaatct
    33181 ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca tgaacctaaa
    33241 ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga attgctaaaa
    33301 gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag ccacaatatg
    33361 gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa aatggttttg
    33421 aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt cttggaatct
    33481 ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat ccttcatgaa
    33541 gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat ctttactcgt
    33601 gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt ggaagagatt
    33661 ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca aagatgtcta
    33721 tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt ttatcatgct
    33781 gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca ataaaatcgg
    33841 cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa cagatgattt
    33901 tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg aagaaaaaat
    33961 cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc tttatagcga
    34021 aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg gcttgcaatc
    34081 gatgcttcga gcgagtcaat taggcatttc accagaagaa gtagctagct tgagccaaca
    34141 agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga cagtcggaga
    34201 agataactcg acagtccaat acgtcgtaag ttatgtagca acgattgtct tgttcattat
    34261 tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag gcacgcggat
    34321 catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa aattaacagg
    34381 tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg gtataggata
    34441 cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct cattggataa
    34501 cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca ttttgatttt
    34561 ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata cagcaaaagc
    34621 aattttgcca gtgacttatc tatctctagg cggatacatg cttggattga tactaggagc
    34681 ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct tatcttcata
    34741 tattatgccg gttcgcttag caaatgagac agtcggtatg ggtggagcat tgatttcctt
    34801 gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca tgtacaaatc
    34861 gaatgtactc gtttatagcg aaggcggatt atggagttca ttgaaacaat cgatttcaat
    34921 tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa atacagaagg aagctggaca
    34981 tctattgttc ccagcttctt tttgtataga cctattttat ttattttact atattggata
    35041 tatattcatt gaaaatagtg tataacaact ttataatatt gagtatacca atttttggag
    35101 gcgttttaat ggaccgttat ttagatttac tttcagaaaa attcccctca acggaggatg
    35161 tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga acagagttat
    35221 ttttgagtga tatacatggc gaatttcctg cttttgatca tattctgcgt atcggttcag
    35281 gaaatcttaa agaaaagatc aaagaccttt tttctgaaca aatgacggaa acggatatca
    35341 gccaattcac tatttttacg gcttatccag aatatgcatt aacgactgat tggtacaaag
    35401 aacaagataa atcgcagctg atccatcatt tgatcgatct tttacggttt acgacagtca
    35461 aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga atatagctat atcattgagg
    35521 aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga ctatgtaaaa aaaatcattg
    35581 aacaacttgt tttgatgaag gaagaagaaa aatttttaac gaaacttgca cagacgatcc
    35641 aaaaatctgt catcgatcat ttgcatatcg ttggagatat ttttgaccga ggaactcaag
    35701 cagccaaagt gatggatcga atcatggatt tttcttctgt agatatccaa tggggaaatc
    35761 atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttactg acacttttac
    35821 ggattgcagc acgctataat tatttatttg aattagagaa agaatatggg ttgaatttac
    35881 gtccattgtt ttcttttgct catcaaactt atcagaagaa tccagtattt gcgcctaaaa
    35941 acagtaaaaa tctatccgaa agagagcaag aagaattaga aaaaatccat caggcacttt
    36001 ctatccttca attcaaatca gaacatcagt tattggatcg tcgacctgaa tttaaaatgg
    36061 acgatcgtaa attgctggaa aaaatcgact atgaagcatc aacgattgat ttagaaggtc
    36121 agctctatga gttgaaagga acttgttttc aaacgatcca gccagatgat ccaaaaaaat
    36181 tcttggaaga ggaacaaaaa gtgattgata gtttgatgta ttcattccag cactctttga
    36241 gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg aatgtatctc acgaacaatc
    36301 agcatgtgct gtttcatggc tgtatacctg tagacgagaa gggacagctc ttagcatttg
    36361 aattagacca atcttatcgc ggaaaagaac tgctgggctt ttttgaaaaa cagataactg
    36421 aaagcgcaaa agatctgact gcaaaagaag atttagcaac cgatttgatt tggtatgcgt
    36481 ggagtgggcc attttcgcct ttattcggca agagtaaaat ggcgactttt gaacgttatt
    36541 ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc ttattatcac ttgcgagatg
    36601 aagagtggtt cagtgaaaaa attttagcag aattcgacac aaaagaagaa gggtcagcaa
    36661 tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga gtcaccgatc aaagcaaatg
    36721 gaaaagtatt tgtcattgac ggaggtttat ccaaagctta tcaaaaaacg acaggaattg
    36781 ccggttattc actattatgc aattcttatg gtttccaaat cgtgacacat tatccgtttt
    36841 tgcctattga acaacaattt gcaaaaaaat ccgaccaaac aaatgtgaaa aaagtaatcg
    36901 aacagcaact accacgcaaa ctcaatcgtg atacaacaaa aggtatggaa ctgcagcagc
    36961 agattaccca gctcaaaagg ctgttggact atcgaaagga cgattgagaa gaattccagt
    37021 gaacattgtt ccatagactg gagactgaga caaaagtcgt gtcgcagtct ctttttccgg
    37081 ataaacggtg ttcttctgcc aactcctcgg tcacaagtca cactattcaa caaacatgaa
    37141 aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct cacgacctct
    37201 tggtataaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg
    37261 tgaaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct ttttcacaac
    37321 cccctcagaa agtctattaa aattcattca caaagaaatc gaataagcgc tgttcagatg
    37381 gatgtacacg gtgggtaagt tccggatgcc attgaacacc gaggatttta tgcatatcat
    37441 ctttgctttg aaccgcttca atgagtccat cgttagaaaa agcgatggct tttaataaag
    37501 gtgaaagatc tttgactgcc tgatgatggt aagaattcac aaaatatgga tcggggagaa
    37561 ggttgcttaa caaactgtct gaaacaatct tgacttcgtg agtcgtaaat tgcggggcag
    37621 tgggctgttg ttcatgtttt actgaccact ttggatataa tgaaagatct tgataaagtg
    37681 taccttccaa aacgacattc aatagttgga gtccccggca gactgcaaaa attggttttc
    37741 tttgtttcaa tgcttccaag atcagtgcct gttcaaattg gtctcgttga atatttgttt
    37801 ccgttagctt tggatgagga tcttccatgt aaagttgtgg actgacatct tgaccacctg
    37861 caagaataag tttgtcgatt tgtccaatca gttgaggagc tgaatctgga gacatgactg
    37921 gcagaataag aggcaatcct tgagcatcta aaactgcatc cacgaatcct tgcggtgtat
    37981 agctcacttg atttccttgg aaagtatctg ttgctcgaat cagttgattt ccggcaatgc
    38041 cgatgattgg tttcatttgt attcccccta aagtagactt attgtaacaa ggatttaaaa
    38101 aaaattaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
    38161 taaagagaaa tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
    38221 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
    38281 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
    38341 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
    38401 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaaa agcgtgctca
    38461 aacactgcgg cagcagctga agatctatgc aacagaaaaa gagcaggaat tggaaacgat
    38521 ccaaacaaac tatcgacaac aactaaaaca gttggtggaa gaaaaagaac aatttgaaat
    38581 acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
    38641 tttgaaagat caatatcaaa agcatcagag tcaagcgatc ggtgcgatca ttgaaagggt
    38701 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
    38761 atcaggagag gattttacaa gcagtacaag gaatccaaaa cgttgaaatc cgggatctgt
    38821 atcaaaatag aacaaacaac cagtgggtgg agaagtattt tccagatacc acgattattg
    38881 ataaagaagc aaaattaagc gctttaggta atcggttgaa agacatccaa gaagctatcc
    38941 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
    39001 tttccttaca agatttggaa gccaattatt ccgaagaaaa tttcataaat aaattgaaag
    39061 aagtccttga tttaaaagaa aagtgggaac aactaactga agaacgtgat cgtttagaag
    39121 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
    39181 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
    39241 aagaactgag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
    39301 tttatctttc gtatgttgct ttaaaaaaat cagttgctgt tatagaagaa attgctggga
    39361 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaactgcaag
    39421 agaccaagca gcaattatcc attgttcacg agcagcaaaa gaagataacg acagcgttag
    39481 gtcagtgcag tggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
    39541 cagaacgaga aaaaataaaa gaacggttta tccatgcgac ttatctgatc gttttgcaag
    39601 gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt gttaaacgaa accgtatcag
    39661 aagaagatat atacgtcagt ttcgaaacac caaactcaga agagattcag caggaagtcc
    39721 ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga attgctgact gaaatgtaca
    39781 gtctgccaaa atatgaagaa gtagatccaa caccttggat gacaccattt tatcttgtct
    39841 tcttcgggat gatggtcgca gatgttggtt atggtttgct gatgctcatt gggacaatac
    39901 tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga
    39961 ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat
    40021 taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg
    40081 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat
    40141 tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt
    40201 ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa
    40261 aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct
    40321 taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata
    40381 atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac
    40441 taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc
    40501 cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
    40561 ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg
    40621 gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg
    40681 tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg
    40741 attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga
    40801 cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
    40861 cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag
    40921 gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg
    40981 atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag
    41041 gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga
    41101 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga
    41161 ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa
    41221 gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag
    41281 agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
    41341 gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa
    41401 ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa
    41461 gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga
    41521 atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt
    41581 aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg
    41641 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
    41701 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
    41761 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
    41821 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
    41881 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
    41941 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
    42001 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
    42061 atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc
    42121 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
    42181 accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac
    42241 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
    42301 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
    42361 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
    42421 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
    42481 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
    42541 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat
    42601 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
    42661 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
    42721 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga
    42781 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
    42841 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
    42901 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
    42961 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
    43021 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
    43081 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
    43141 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
    43201 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg
    43261 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
    43321 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
    43381 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
    43441 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
    43501 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
    43561 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
    43621 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
    43681 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
    43741 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
    43801 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
    43861 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
    43921 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
    43981 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
    44041 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
    44101 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
    44161 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
    44221 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
    44281 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
    44341 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
    44401 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
    44461 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
    44521 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
    44581 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
    44641 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
    44701 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
    44761 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
    44821 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
    44881 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
    44941 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
    45001 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
    45061 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    45121 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    45181 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    45241 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    45301 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    45361 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    45421 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    45481 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    45541 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    45601 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    45661 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    45721 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    45781 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    45841 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    45901 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    45961 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    46021 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    46081 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    46141 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    46201 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    46261 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    46321 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    46381 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    46441 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    46501 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    46561 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    46621 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    46681 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    46741 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    46801 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    46861 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    46921 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    46981 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    47041 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    47101 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    47161 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    47221 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    47281 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    47341 catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc
    47401 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    47461 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    47521 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    47581 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    47641 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    47701 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    47761 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    47821 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    47881 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    47941 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    48001 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    48061 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    48121 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    48181 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    48241 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    48301 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    48361 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    48421 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    48481 attcctttat ttatcaatag gttataattc atattttttt attttttctt ttacgatact
    48541 tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc
    48601 tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa
    48661 gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg
    48721 gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt
    48781 tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt
    48841 gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat
    48901 cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa
    48961 ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat
    49021 gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag
    49081 accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc
    49141 tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc
    49201 ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa
    49261 atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct
    49321 atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac
    49381 aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt
    49441 agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg
    49501 gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact
    49561 aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc
    49621 aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc
    49681 aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag
    49741 ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc
    49801 taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca
    49861 agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat
    49921 accatacaat tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga
    49981 attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc
    50041 gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc
    50101 tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc
    50161 ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca
    50221 tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact
    50281 acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc
    50341 gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg
    50401 tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt
    50461 tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta
    50521 tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat
    50581 catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga
    50641 cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt
    50701 gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga
    50761 cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg
    50821 cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga
    50881 cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga
    50941 gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg
    51001 ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg
    51061 caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa
    51121 gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca
    51181 ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt
    51241 tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta catggatttt
    51301 cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct
    51361 aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg
    51421 gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc
    51481 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    51541 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    51601 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    51661 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    51721 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    51781 accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    51841 ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    51901 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    51961 agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccgg cagctttatg
    52021 ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc
    52081 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catccccagg
    52141 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    52201 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca
    52261 tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa
    52321 gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    52381 gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa
    52441 gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca
    52501 gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg
    52561 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    52621 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    52681 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    52741 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    52801 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    52861 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    52921 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    52981 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    53041 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    53101 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    53161 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    53221 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    53281 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    53341 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    53401 cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga
    53461 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    53521 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    53581 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    53641 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    53701 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    53761 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat
    53821 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    53881 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    53941 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag
    54001 aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt
    54061 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    54121 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    54181 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    54241 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    54301 actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat
    54361 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    54421 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    54481 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    54541 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    54601 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    54661 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    54721 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    54781 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    54841 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag
    54901 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    54961 gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat
    55021 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    55081 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    55141 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    55201 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    55261 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    55321 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg
    55381 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    55441 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    55501 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    55561 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg
    55621 aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt
    55681 ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga
    55741 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    55801 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    55861 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    55921 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    55981 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    56041 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    56101 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    56161 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    56221 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    56281 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    56341 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    56401 gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg ttcatgggac
    56461 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa
    56521 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    56581 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    56641 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    56701 tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    56761 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    56821 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    56881 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    56941 acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg
    57001 atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact
    57061 tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg
    57121 aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat
    57181 tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat
    57241 cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata
    57301 aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg
    57361 tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg
    57421 gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat
    57481 gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga
    57541 tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc attttagtgc
    57601 atggttttat cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac
    57661 gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt
    57721 tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct
    57781 ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata tgaatgaaaa
    57841 cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag
    57901 gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata
    57961 aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag
    58021 agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc ggagtatctt
    58081 tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg
    58141 cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg
    58201 atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa
    58261 ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa
    58321 aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc
    58381 agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag
    58441 cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct
    58501 cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa gaagtcaaac
    58561 ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata
    58621 ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga
    58681 acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg
    58741 gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt
    58801 atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca
    58861 agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt
    58921 tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt
    58981 gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca
    59041 atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat
    59101 tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag acagtacaaa
    59161 aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc
    59221 gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt
    59281 cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg
    59341 gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt ctgcccagtc
    59401 gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg gcaaaacccc
    59461 cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga tcatccagcg
    59521 accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc ctgatttgat
    59581 tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc atgatatcta
    59641 tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc aagggtatcg
    59701 catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact attatggcag
    59761 aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata acacatttta
    59821 ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc tatacaggta
    59881 caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa aataaagaag
    59941 caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga atcgaacccc
    60001 cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa gcactgggat
    60061 aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg aaatttgagc
    60121 aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa cagttgcaac
    60181 agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt gagaatcaag
    60241 caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat tacataaaag
    60301 cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt cctgatacaa
    60361 aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga gatacttttt
    60421 tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt ttgacgatta
    60481 gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc gatgcgttga
    60541 ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa gaatgtctca
    60601 tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg ttggaagaat
    60661 gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc
    60721 tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc cctggccgga
    60781 ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta gtggacgacg
    60841 ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga
    60901 gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat ttaaaagaac
    60961 ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat acaattttgc
    61021 gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag gaacgtccaa
    61081 tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa tccacggtca
    61141 gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa ttaaaaaaat
    61201 ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt tcagcagata
    61261 cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag ccgttgtctg
    61321 accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga cgaacagtag
    61381 caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt tatgataact
    61441 aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag ttgaaaaacg
    61501 agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt gatgtgaaat
    61561 tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac attggacgta
    61621 aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga atctgttgca
    61681 ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat ctattggatg
    61741 cagccgattg ggcgtcgaag tttatcggaa agtatgggga tcacagaacg agtcttacga
    61801 acggaaacag accttttgaa aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg
    61861 ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga tcagctttta
    61921 ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg ctgtatcgtt
    61981 gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga agcgctgagc
    62041 gaagccttag accgactact tccagaaggc gagaatatca tcgctgtgat gggaggaact
    62101 actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg gcataacctg
    62161 tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa ttctgtcagt
    62221 gcaagaatgg ctgctaaagc aaacggtaac caccgcgcac tttatgttcc ggaacaatta
    62281 agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt cttaaacttg
    62341 attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat ggcagcacgt
    62401 cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt tgcggaatct
    62461 tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag aatcggcata
    62521 gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa
    62581 gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct catcactgat
    62641 gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata tttttttgat
    62701 tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt ttggacgtat
    62761 tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag tagcaatcaa
    62821 cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg
    62881 tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa
    62941 agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg tagatatcgt
    63001 tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt taacagctgg
    63061 tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa tcgtttatca
    63121 cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt catgtactac
    63181 taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt
    63241 aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac ctcatcctaa
    63301 aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact caacaggtgc
    63361 tgctaaagct atcggtttag taattcccga attgaacggt aaattagacg gagctgctca
    63421 acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg aaaaagaagt
    63481 aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt acggatacaa
    63541 cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat tatttgatgc
    63601 tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg ttgcttggta
    63661 cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact tcgctaactt
    63721 ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa gcggggaagc
    63781 aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat aaaatggcta
    63841 agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt gttgacttca
    63901 acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca gcattgccaa
    63961 caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac cttggacgtg
    64021 taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg
    64081 aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa ttagaagaag
    64141 caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga tttgaagata
    64201 ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg gcttcattag
    64261 gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct tctaacgtag
    64321 gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa gaaatcaagt
    64381 tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa
    64441 aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat aaaatcttga
    64501 tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc ggtaactctc
    64561 tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca ggcgacaaac
    64621 ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt gaaacacaag
    64681 taatcgaagg agacattcct gatggtttga tggctttaga catcggccca gcatcagtaa
    64741 aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga cctatgggtg
    64801 tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca atcgctaact
    64861 tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca gaacaattag
    64921 gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg gaattgcttg
    64981 aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc aagtttgctc
    65041 aattctgacg agtaatcgaa caaaaggtga agggagtttt catgactccc tagccatttt
    65101 tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa
    65161 atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct
    65221 tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca agaattaatg
    65281 acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg ggaaaactca
    65341 ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt tcattatgtg
    65401 atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga catcaataaa
    65461 aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg cgaatcttta
    65521 gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac agctggttta
    65581 gctgatcttt cagctgaaca ggtgtcaaac atggttattg cttatgaacc aatctgggca
    65641 atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt
    65701 caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat ccaatacggc
    65761 ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt tgacggtgct
    65821 ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga cgctgtaaaa
    65881 taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc aaaattaact
    65941 aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga gacaaaacat
    66001 gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta acccaacaat
    66061 cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc cttcaggtgc
    66121 ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc gttatggcgg
    66181 taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag caatcattgg
    66241 ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag acggaactcc
    66301 taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc
    66361 agctgctgac tacctagaag tacctttata ccactaccta ggcggattca acacaaaagt
    66421 attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata actcaatcga
    66481 cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat
    66541 gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat tagcaacttc
    66601 tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag gttttgaagt
    66661 aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg ttcttgctat
    66721 ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac tagctgactc
    66781 aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat tagtttctaa
    66841 atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg gattcaagaa
    66901 attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt tcgtaacaaa
    66961 cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt
    67021 gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta aagaagctgg
    67081 ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa tctctgatat
    67141 cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta ctgaccgtat
    67201 tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg cagaatacaa
    67261 aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg aagatacatg
    67321 atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa ctctctactt
    67381 gctgaaatat cagcaggtag agagtttttt tgcaactagc ttctgtatat gatgtttaca
    67441 aagaatggta caagaaaaga tcatccttga aaaaggaaag ctaacaataa ctagcatcat
    67501 ataagagctt ttaaggatag aacagtagaa taggaatcta tgggcaagtc ctctcgtata
    67561 aata
//
LOCUS       DACDHL010000006        64620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDHL010000006 DACDHL010000000
VERSION     DACDHL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.006"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            19..657
                     /locus_tag="IV228_000403"
     CDS             19..657
                     /locus_tag="IV228_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8716348.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(776..848)
                     /locus_tag="IV228_000404"
     tRNA            complement(776..848)
                     /locus_tag="IV228_000404"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(813..815),aa:Thr,seq:cgt)
     gene            969..1211
                     /locus_tag="IV228_000405"
     CDS             969..1211
                     /locus_tag="IV228_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716349.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(1350..1979)
                     /gene="upp"
                     /locus_tag="IV228_000406"
     CDS             complement(1350..1979)
                     /gene="upp"
                     /locus_tag="IV228_000406"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8716350.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(2113..3060)
                     /locus_tag="IV228_000407"
     CDS             complement(2113..3060)
                     /locus_tag="IV228_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8716351.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(3213..4457)
                     /locus_tag="IV228_000408"
     CDS             complement(3213..4457)
                     /locus_tag="IV228_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8716352.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(4648..5673)
                     /locus_tag="IV228_000409"
     CDS             complement(4648..5673)
                     /locus_tag="IV228_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8716353.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(5695..5874)
                     /locus_tag="IV228_000410"
     CDS             complement(5695..5874)
                     /locus_tag="IV228_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716354.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(5867..6706)
                     /gene="prmC"
                     /locus_tag="IV228_000411"
     CDS             complement(5867..6706)
                     /gene="prmC"
                     /locus_tag="IV228_000411"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8716355.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(6699..7772)
                     /gene="prfA"
                     /locus_tag="IV228_000412"
     CDS             complement(6699..7772)
                     /gene="prfA"
                     /locus_tag="IV228_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8716356.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(7775..8359)
                     /locus_tag="IV228_000413"
     CDS             complement(7775..8359)
                     /locus_tag="IV228_000413"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8716357.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(8465..8934)
                     /locus_tag="IV228_000414"
                     /pseudo
     CDS             complement(8465..8934)
                     /locus_tag="IV228_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9040..9642
                     /locus_tag="IV228_000415"
     CDS             9040..9642
                     /locus_tag="IV228_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8716358.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(9746..10315)
                     /gene="lepB"
                     /locus_tag="IV228_000416"
     CDS             complement(9746..10315)
                     /gene="lepB"
                     /locus_tag="IV228_000416"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8716359.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10545..11312)
                     /locus_tag="IV228_000417"
     CDS             complement(10545..11312)
                     /locus_tag="IV228_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716360.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(11309..11788)
                     /locus_tag="IV228_000418"
     CDS             complement(11309..11788)
                     /locus_tag="IV228_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8716361.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(11913..12650)
                     /locus_tag="IV228_000419"
     CDS             complement(11913..12650)
                     /locus_tag="IV228_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8716362.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            12811..14211
                     /locus_tag="IV228_000420"
     CDS             12811..14211
                     /locus_tag="IV228_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8716363.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            14434..15786
                     /locus_tag="IV228_000421"
     CDS             14434..15786
                     /locus_tag="IV228_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8716364.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            15779..16477
                     /locus_tag="IV228_000422"
     CDS             15779..16477
                     /locus_tag="IV228_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HAP8716365.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPFGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(16539..17690)
                     /locus_tag="IV228_000423"
     CDS             complement(16539..17690)
                     /locus_tag="IV228_000423"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8716366.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(17703..18140)
                     /locus_tag="IV228_000424"
     CDS             complement(17703..18140)
                     /locus_tag="IV228_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8716367.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(18155..19921)
                     /locus_tag="IV228_000425"
     CDS             complement(18155..19921)
                     /locus_tag="IV228_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8716368.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20185..20862
                     /locus_tag="IV228_000426"
     CDS             20185..20862
                     /locus_tag="IV228_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716369.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            20865..21992
                     /locus_tag="IV228_000427"
     CDS             20865..21992
                     /locus_tag="IV228_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716370.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(22192..22632)
                     /locus_tag="IV228_000428"
     CDS             complement(22192..22632)
                     /locus_tag="IV228_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8716371.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(22643..24676)
                     /locus_tag="IV228_000429"
     CDS             complement(22643..24676)
                     /locus_tag="IV228_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8716372.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(24678..26381)
                     /locus_tag="IV228_000430"
     CDS             complement(24678..26381)
                     /locus_tag="IV228_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8716373.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(26475..27425)
                     /gene="manA"
                     /locus_tag="IV228_000431"
     CDS             complement(26475..27425)
                     /gene="manA"
                     /locus_tag="IV228_000431"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8716374.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(27464..28489)
                     /locus_tag="IV228_000432"
     CDS             complement(27464..28489)
                     /locus_tag="IV228_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8716375.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(28698..29567)
                     /locus_tag="IV228_000433"
     CDS             complement(28698..29567)
                     /locus_tag="IV228_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8716376.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(30271..32163)
                     /locus_tag="IV228_000434"
     CDS             complement(30271..32163)
                     /locus_tag="IV228_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716377.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(32160..33887)
                     /locus_tag="IV228_000435"
     CDS             complement(32160..33887)
                     /locus_tag="IV228_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716378.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(33903..34553)
                     /locus_tag="IV228_000436"
     CDS             complement(33903..34553)
                     /locus_tag="IV228_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8716379.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            34757..35053
                     /locus_tag="IV228_000437"
     CDS             34757..35053
                     /locus_tag="IV228_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8716380.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            35245..35865
                     /locus_tag="IV228_000438"
     CDS             35245..35865
                     /locus_tag="IV228_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8716381.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(35957..37123)
                     /locus_tag="IV228_000439"
     CDS             complement(35957..37123)
                     /locus_tag="IV228_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8716382.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(37135..37488)
                     /locus_tag="IV228_000440"
     CDS             complement(37135..37488)
                     /locus_tag="IV228_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8716383.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            37616..38638
                     /locus_tag="IV228_000441"
     CDS             37616..38638
                     /locus_tag="IV228_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8716384.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(39077..39511)
                     /locus_tag="IV228_000442"
     CDS             complement(39077..39511)
                     /locus_tag="IV228_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716385.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(39621..41480)
                     /locus_tag="IV228_000443"
     CDS             complement(39621..41480)
                     /locus_tag="IV228_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8716386.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(41598..42758)
                     /locus_tag="IV228_000444"
     CDS             complement(41598..42758)
                     /locus_tag="IV228_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8716387.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(43065..44489)
                     /gene="araA"
                     /locus_tag="IV228_000445"
     CDS             complement(43065..44489)
                     /gene="araA"
                     /locus_tag="IV228_000445"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8716388.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(44505..45203)
                     /locus_tag="IV228_000446"
     CDS             complement(44505..45203)
                     /locus_tag="IV228_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8716389.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(45160..46821)
                     /locus_tag="IV228_000447"
     CDS             complement(45160..46821)
                     /locus_tag="IV228_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8716390.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            47039..48439
                     /locus_tag="IV228_000448"
     CDS             47039..48439
                     /locus_tag="IV228_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8716391.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(48691..49770)
                     /locus_tag="IV228_000449"
     CDS             complement(48691..49770)
                     /locus_tag="IV228_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8716392.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(49856..50815)
                     /locus_tag="IV228_000450"
     CDS             complement(49856..50815)
                     /locus_tag="IV228_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8716393.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(50827..52350)
                     /locus_tag="IV228_000451"
     CDS             complement(50827..52350)
                     /locus_tag="IV228_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8716394.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(52360..52924)
                     /locus_tag="IV228_000452"
                     /pseudo
     CDS             complement(52360..52924)
                     /locus_tag="IV228_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(52930..53793)
                     /locus_tag="IV228_000453"
     CDS             complement(52930..53793)
                     /locus_tag="IV228_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8716395.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(53807..54703)
                     /locus_tag="IV228_000454"
     CDS             complement(53807..54703)
                     /locus_tag="IV228_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8716396.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(54878..56149)
                     /locus_tag="IV228_000455"
     CDS             complement(54878..56149)
                     /locus_tag="IV228_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8716397.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(56139..57656)
                     /locus_tag="IV228_000456"
     CDS             complement(56139..57656)
                     /locus_tag="IV228_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8716398.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            57854..58744
                     /locus_tag="IV228_000457"
     CDS             57854..58744
                     /locus_tag="IV228_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8716399.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(58817..59641)
                     /gene="nadE"
                     /locus_tag="IV228_000458"
     CDS             complement(58817..59641)
                     /gene="nadE"
                     /locus_tag="IV228_000458"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8716400.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(59653..61125)
                     /locus_tag="IV228_000459"
     CDS             complement(59653..61125)
                     /locus_tag="IV228_000459"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8716401.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(61762..62124)
                     /locus_tag="IV228_000460"
     CDS             complement(61762..62124)
                     /locus_tag="IV228_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8716402.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            62347..63099
                     /locus_tag="IV228_000461"
     CDS             62347..63099
                     /locus_tag="IV228_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8716403.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(63239..64162)
                     /locus_tag="IV228_000462"
     CDS             complement(63239..64162)
                     /locus_tag="IV228_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8716404.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(64177..64449)
                     /locus_tag="IV228_000463"
     CDS             complement(64177..64449)
                     /locus_tag="IV228_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716405.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18617 a        13300 c        11271 g        21432 t
ORIGIN      
        1 atagtaggag ttattatcat gaataacatt ttaggaaaaa aaatagaact taaaaaaggg
       61 atattgggca ctattgtcgt tgtactcggt ttgttagggt tgctatttgc gccatccatt
      121 attagcattt tttctttaac tatcactgga tttatagcat ttacacttgt ttttggtaaa
      181 aaagatacga aaaaaatgtt ctcgaaacct gtcgctcctg tcaaaaatat agccaaatat
      241 ttttttatca atgtagtaat cagtgccgca gtctcggtat ttttgcaaca aattttgaaa
      301 tggggtttaa caggcaatcc aataaatgag gctttcagcc cgttattatt tattattttg
      361 cctatcatga tacttggaga agaattgttc tcagtgtatt ttctcgctat tttttctagt
      421 aagttcagca ttcctgtcgc tagtttcttg tcagcaatca tctttggatt tatccattac
      481 agtacttatg ataatggaaa tattttacac actgtagcac acatcctttt gatacaaggg
      541 gtggcaagat tgttattcaa ccaagctgcc attaaaagca actcgatcat tacaagttgg
      601 gcagttcatg tgatatttga tttcacagct atattgctag tcgttttatt ctcctaaatt
      661 tttatagaaa taatacatgc tctattgcta agaaagcata ctttatttcc tttaaacaaa
      721 aagacagatg tatcaatgga ttaaaaatcc ttgatatatc tgtcttctct tttaatgcca
      781 gctataggga tcgaacctac gacctacgcg ttacgagtgc gttgctctac caactgagct
      841 aagctggcct tatccaaaag aaattatata ctctatttgg ctgattgtaa agagtccgat
      901 ggaaaattag tttaatttca tgatattttt cataaaacgt ggtataattg atgtgaaagg
      961 caggttttat gaaaatggca acttttggaa aatttgatac aactatcgta ccatcggaaa
     1021 ttggtgaaaa attcaagatc aatactcacg taatgttcac aattcacggc aagtcgcaaa
     1081 ctggaacgat ttcaaagcaa ttgaaaaatt ccgctgttgt tgagattgat gaaactgcag
     1141 aaaatgcgaa cttgatgacg caaagtaagg gtgtagtaat cgttaactac aaagaactaa
     1201 aaaagatttg aatttgacca tctctgcatt tttgggatgg tttttttata tgcaaacgaa
     1261 agctgtgaaa aagctgttct gcatcaaacg ttaaaaatga ccaaaaaagg atgtgacaaa
     1321 agttgtgtca cacccttttt cgtatttact tattttgtac caaacaaacg gtcacccgca
     1381 tcccctagtc caggaacgat ataaccatgt tcattcaatt tttcatctag agaagctgta
     1441 tagatgtcaa tatcaggatg cgcttcttgc aaagctttta ctccctcagg tgctgctacc
     1501 aaacaaacaa atttaatgtt cgacgcgcca cgttctttca atgcatcgat tgccatgatt
     1561 gctgatccgc ctgttgccaa cattggatct actacaaaca actgacgtgc atcaatgtct
     1621 tctggaagtt tgacgaaata ttcatgtggt tccaatgttt cttcatctcg ataaagaccg
     1681 atatgtccca ctttagcagc tggaatcaat tccaagatac cgtcaaccat tccaatacct
     1741 gcgcgcaaga ttggaataat cgctactttt ttaccagaca acgttttttg tgttgtttca
     1801 gtgatcggtg tttcgatcac gatgtcttcc aatggcatat cccgtgatac ttcatatgcc
     1861 atcaacatag cgatttcatc gactacttca cgaaaaacct ttgttccgca gtttttgtcc
     1921 cgaataatag ttagtttgtg ttgaatcaat ggatgatcga tcacttgaaa tttgcccatt
     1981 ttttaagctc tcctttgttc tgtcgtttct ttttaattgt aaaacaaaat caagtgattg
     2041 actagtcaaa ctcgccattt ttcctaagaa aatccgtttt tgaaaagaag ttttcctgcg
     2101 gacaagcaaa gcttattctt ttttcttgat ccaagaagta gataatgcca ttgctgcacg
     2161 cgttgccttt gtctgatcca tagcatttac catgacaaaa tcatggatga ttccttggaa
     2221 gcgtacttgt gtaacaggaa cccctgcatc acgaagtttg cgcgcataag cctcaccttc
     2281 atctcttaat acatccgctt ctcccgttaa aatcattgct gaaggaagat catttagttc
     2341 atttatattt gctctcaaag gcgaagctgt gatttcagca cgctcttctt cattcgtcgt
     2401 atattggtcc caaaaccaca tcattccttc tcttgtcaaa aaatagtttt ctccgaattc
     2461 atgataggaa tctgtatcaa aatcagcgtt tgtgactgga taatacagca attgcttgga
     2521 tattgggagg ccctttcgtt cttttgttaa gatcgtcatg actgtagcca tgttaccacc
     2581 tactgaatct cctgcaacga ttagattctc gagatccaga ttttttgact tggctaattt
     2641 attcagctgt tgcaacacag cataattttg ttcaattgct gttggatatt ttgcttctgg
     2701 tgatcgacta tattctggaa agacaacgac ggaattcgta cgaaccgcaa gttctctgat
     2761 caatttatca tgggtttgag cactgccaaa aacccagcct gctccatgaa tataaaaaat
     2821 aactggtaat ttatccatat tcccttctgg tcgtacgaga cgaatattga gttttcccca
     2881 ttcgccagta tccaccatca agtcttcaat atcgacggaa tatttgttca caggtgattc
     2941 ttgtacttct tctagtaaaa ctcttccttc ttccggtgct agttcataga tacgcggatg
     3001 aggcgcattt gcatcactga atgctactgc ttccttttct aacgagatac gatcagtcat
     3061 gacttttcct ccctgagatt tagtgtgaag actaaaatta cacctcatat actagagagc
     3121 atcaccgata ttgtcaaaga atccgttttc ccaaaaaata aacagaaaag cccaacattg
     3181 aacttttctg tttactaatg gaatttaaat ttttattttt tatgcaacgg aaatttttca
     3241 gttaattcgc gtacacctgt tttgacttcg tctagttgag cgttatcatc ttttgcttgt
     3301 aatgctttga caactagttc agccactttt acagcatctt cttctttgaa cccgcgagta
     3361 gtgattgctg gcgtgccgat tcgaataccg cttgttttga atggacttag tgtttcaaaa
     3421 ggaatcgagt ttttatttac agtgatattc acactgtcca aaatgctctc tgcttctttt
     3481 ccatttatac ctaactcgcg aacatcgatc aacatcagat gattatctgt cgcccctgaa
     3541 atgactctgg ttccaattgc ttgattgaaa actttgacca ttgcttttgc attggcgatg
     3601 atttgttcac tatattcttt aaatgcagga tctaatgctt ctttgaatgc tactgcttta
     3661 cccgcaatca catgctctag agggccgcct tgaataccag gaaataccgc actatttatt
     3721 ttttttgcta atgcttcatc gtttgtcaaa atcatgccgc ctcgagggcc acgcaatgtt
     3781 ttatgagtgg tagtcgttgt gatatctgca taaggaactg gattaggatg aagtcctgct
     3841 gctactaatc ctgcgatatg tgccatatct accatcagtt ttgcgcctac ttcatctgcg
     3901 atttctctga atttcgcaaa gtcgatcgtt cgtccgtaag cactggcacc tgctacgatc
     3961 aattttggtt gatgttttct agctaaaata cgtaccacat tataatcaat aacttctgtt
     4021 gtcggatcaa caccgtaagc aacaaagtga taggttttgc cactgaagtt gactggtgat
     4081 ccatgtgtta gatgtccgcc tgcggaaaga tccattccta agatcgtgtc ccctggttct
     4141 accaaagcga gataagcagc cgtgttggct tgagagccgg agtgaggctg gacgttcgca
     4201 aatttcgctc cgaataattc ttttgcacgg tcaatcgcta aattttcaac gatatcgaca
     4261 aattcacacc caccataata gcgatgccct ggataacctt ctgcgtattt atttgtcaag
     4321 atacttcctt gtgcagccat cactgcttcg gagacaaagt tttcagaggc aatcagctcc
     4381 aaattatgtt cttgtctttc ttcttctttg gcgatcgcag cccaaagatc agggtcaaac
     4441 gttttgtaat ctaccacaaa caacagctcc tttttatttt cctacaattt tattttccta
     4501 caattttatt tttcccatca aaaacaaatt gccaaatatt cgtatctctt ctattacgat
     4561 tagttacgct tagtttttcg ttcactgata gaagatttac ttgatagcat tgtatcatac
     4621 taaaagaatg cgacaataac caattgatca tttttcaaaa taattttgtc ctgctgcttt
     4681 tttcaagcga ttcatgtaag cattccccaa tccagtttct ggatacactt gtacaaaaat
     4741 caaatctaat ccaagtgttg gttcatctaa tcctcttaga cctgcaaaca atcctttagc
     4801 cgccgcttct actgagttgt cgttatacat ataaacagct gcagtatctg tccgcacttg
     4861 atccgcaatt tcggggcttg ctatgacacc tgcacggatc ttcttatttt ttgcccattg
     4921 aactgcagtt gaccaatcgc catctctgac catcagaaca cgtgtatcgg gtgagtagtg
     4981 tttgtatttc atcccagggg cttttggtgt ttcattttct tttacaagat gctgatcaat
     5041 agcaacagga gattcgatga ctgcttcgat ttgttctttc gttactgcac ctggtcgtaa
     5101 aatcataggc atggctgttg gatcacttaa atcaagaaca gtggattcta caccaattcg
     5161 cgtagctcca tcatcaataa tcccggtaat ttttccttgt agatcatgat atacatgatc
     5221 tgcagtcgtt ggacttggtt ttcccgacgt atttgcactt ggaccgacaa gcggaacacc
     5281 ggaaagttcg attacttcta gtgttttttt gttatccggt atacgaaatg cagctgtgga
     5341 taatcctccc gtcacaactg agggcaaagt gtcttttttg atcttaaata tcaaagttaa
     5401 tggtccaggc cagaagttct tcacaatctt ctctgctgcc ggatgaaagt tgtccacata
     5461 ctcttttact tgttcaaaag aggcgacatg gacgattaat ggattatctt gcggtctccc
     5521 ttttacagaa aacactttct tgacagcgtt tggtaaaaga gcgttggcgc ccaatccgta
     5581 tactgtttcg gtaggaaaag caacaagttc tccttctttc agtgcttcgg ctgcctcttg
     5641 caattctgtt tcatcatatc gtttcgtttc cacagtttat ccacaacccc tttttcatac
     5701 attcttattt tgtctgagtt tatcctcaag ttttccactg tttgtggata atctatttgt
     5761 caatagtgga taaacttgtt gataaaccaa aaagtcgttt acatccttgt caaataagtg
     5821 tttttttata ataaaaaaac aaataaaaaa tttttcatcc acaacttcaa ccataaactt
     5881 ggatcattcg ttcattgcca aaaagatcct tttttatcgc cactttttta tctggaaatg
     5941 cttgctgaaa tatttttttt actgcttcac cttggcgaaa tccgatttct aaaaaaatct
     6001 ttccatttga ttttagtacg cttggtgctt caactgctat tttttcataa atagctaatc
     6061 catcgttctc agcaaaaagg gccattttag gttcgaatga ccggacactt tcatccatca
     6121 gttcccattc attccgactg atgtacggcg gattagaaat aatcacatca aattgctgat
     6181 ccattactgg tcctaatgta tctccatgat aaaaactgat tttcgttcct tctttctgag
     6241 cattttcttt cgctacttct aatgcttctt ctgaaagatc cacagcagat atatgccaat
     6301 tttttcttgc tgctttcaaa ctaatagcaa ttgctccagt ccctgtccca atatctatca
     6361 cttctaacgg tacaccaggc gtctcgtcta aacaccattc gactaactcc tctgtttctg
     6421 gtcgagggat cagtgttgct tcagttacct ttaaccgatg atcataaaaa tctgcatatc
     6481 ctagcaaata ctgaggtgga tgatctgcaa gcagaagtcg cagatcggtt tcgatcaacc
     6541 gctgttcttc ttctgaaatc tcttcgttca tatgaaggag ccaatcaagt tttttccatt
     6601 gttttcgttc caaaaataaa aattggatac tatatgcttc tttcccatga tcctctaaaa
     6661 aagaagaagc ccgtgacagg acttctcgat aggtcatttt acccattttg catagcttcc
     6721 agttttgatg tttgatcata catgatcaat gcatcaacga tctcatctaa tttccctgct
     6781 aaaatctgat ctaatttttg gatagttaat ccgatacgat gatcagtcac acgattttgc
     6841 gggaaattat acgtccggat ccgttctgaa cgatccccag ttccgactgc tgattttcga
     6901 ttggcatcat actcgctttg tgcttcttgc tggatcttat cgtacacacg agcacgcaag
     6961 attttcattg ctttttcacg gttcttgatt tgagaacgtt catcttgcat agcaacaacg
     7021 atccctgttg gtaagtgagt caaacgtaca gcagatgctg ttttattgac gtgctgtccg
     7081 ccagcacctg aagcatgata gatatctgta cggatatctt tgtcttcaat atcgatctct
     7141 acttcttcag cttctggcat cacgacaact gttgcggtgg aagtatggat acgcccttgc
     7201 gattcagttg aaggaacacg ctggacgcgg tgcgcaccac tttcatattt taatttcgaa
     7261 tatacgttgt ctccggtgat catcatgatc acttctttat atccaccgat tccggtaacg
     7321 ttagcttcca tcacttctgt tttccagcct tgtgcttcag catacttttg gtacatattg
     7381 aaaagatcgc cagcgaataa tgctgcttcg tctccgcctg ctgctccacg gatttccatg
     7441 atgatatttt tatcgtcatt tggatctttt ggtaaaagca agatcttgat tttttcttct
     7501 aagacttctt tttctttttt taattcagat aattcttctt ttgctagttc agccatttca
     7561 tcatccagtt tttcaccaag cagttcttct gtatcggtga ttccttgtac tacttcttta
     7621 taacgacgat aaacttcgac tgtttcgcgc gtgtttgctt cttcttttga aagctgcata
     7681 aaacgctttg tatcagaaat cacttctggg tcacttaaaa gttctccaag ctcttcataa
     7741 cggtcttcaa ttgcttgtaa ttgatcgtac atgcttattc tccttctgta atcattgatg
     7801 ggtgaaagta atgttttcga caaacggggt aataagcttc atttccgcca atttgcactt
     7861 gatcgccttc ataaacaggg tttccatcaa tataatgtaa attcatgatt gcttttttgt
     7921 ggcagaacca acagattgtt ttcatttctt cgattttgtc tgcataaagc aataaatatt
     7981 ttgatccttc aaataactcg tttcgaaaat catttttcaa accaaatgcc ataaccggaa
     8041 tatccaattc gtccacaatt ctcgtaaaag caatgacatg tttctttttt aagaattgcg
     8101 cttcatcgat cagcacacaa tagggtttgc tttccatttt ctgaattacg tcaaatacgt
     8161 ctgtttcttc aaatatcggc atcgcattac gtttcagtcc gattcggctt gaaacaacgc
     8221 ctaaaccatc tcttgtatca ataccgcttg tcattaagac aactggctta ttttgttctt
     8281 cataattatg tgcgactttt aggatctcga tcgtcttgcc gctattcatt gcgccatact
     8341 taaaaaatag ttgtgccatg ctctttttca ccttccagtt tgttcctatg gtattatact
     8401 tgattttcag cattttttac agtgagaatt ttcatcaagc acagattagt attaaatcta
     8461 ttgctcactt ttgttccaat tttttgacaa gaactaaaca tgggttccat tcatcccata
     8521 aatttggaaa aacttccaac cttgaaaatc ctaatgattc gtagaaacaa atagtttgat
     8581 catatataga gtagtgcccc tcgtctacag tcttcacttg caaatatttg taatgttttg
     8641 ctgcgtaacc ttctgcttct ttaaaaagtt ttgttcctaa tccttggtga tgatagtctt
     8701 tttttattcc catacaatag aatagatttc ggcagtatct tcacttgtct gtgaaagagt
     8761 gataaagcct aaatattctt cttttctttt ttcacaccat aaaggtagtt tacttgattc
     8821 ctcgatatat ttttccgttg attcaggaag cccaaaccac tcaggcaagt ctagtaatat
     8881 ttctcggaca cactttcctt tttcctctct atcttttaca aacgatatcg gcatattctt
     8941 tctagattct ccctttttga attgagtata gcatgctttc tttcttttca cgtgctataa
     9001 taagttaaaa tttcaaagtt ttacgtaagg agctatttga tgacaaaaaa tatttttgat
     9061 tccatcgcta accattatga ttcccctgac cgtcaagcat tagccaagat tattcgcgaa
     9121 gaactaacta cttatctacc gcgcgacagc caccaaaaag tcctgctgga ttacggaggc
     9181 ggaactgggc ttgttagttt accgcttgcg gaaagattca aagaactgat cattgccgat
     9241 gcttcagaaa ctatgctgaa aatggcagaa gaaaagatcc aagcagcaga tctgaaaaat
     9301 gtccgaacga tccacgctga tgcttctgtc gaatttcctg ctgtccaagc taatctcatc
     9361 cttctatcac ttgtactttt acacattcct gatacggaga atattcttac aaaactttat
     9421 gaaattttag ctcctggcgg tcaattgatc atcgtagact ttgacaagaa tgaacaaatt
     9481 agtcatccga aagtccataa tggctttagc caagaagaat tgaataatcg attggaaaaa
     9541 acgggctttg tctctgctac tagtcatacc ttccatcgtg gggaaaagct atttatgaac
     9601 aaaaacgctt cgcttttctt atccatcagc caaaaagctt aaaatgagat tgcaaaaaaa
     9661 gtgagcaact ccaagaaata agaaggaatt ttcggaaaaa gaagctgtga caaaaaacat
     9721 gtcacagctt ctttttattt tttcatcaca tgattttaat ttctgaaaga ggccaataac
     9781 ggaaaacaac ttttccttgg atcatatctt tgtcgactaa cccaaacatt cggctgtctt
     9841 ttgaaatcaa gcggttatcg cctagaacaa gatactttcc ttttggcaca gtatattcaa
     9901 agtcactcgt aaaggtggat gaactttctg cttgtgcttg gaatcctgag ctataagcat
     9961 attctccttg taacttatct tctgaaaatt cttttttgaa ctcatccaga taaggttcat
    10021 catacttttt gccattaatg gtcaactggt cattttccat cttgactgta tctccaggca
    10081 taccgatgat ccgtttaaca atcatccgtt cttcatcccc cggttccttt gtagtaataa
    10141 tatccattcg ttcatagttt gagatcttag atgaaatcaa tcgttgtcca tcacttaatg
    10201 tagggtccat cgaatgcccg tttacagtaa ctggtgtaaa cacgtacact ctagctaata
    10261 aaatagccgc taatacaagg atcaaaatcc cccaattttt caaaaattct ttcacagctt
    10321 acctcctact tgtattttcc tcgttattta ttacgttttt tcacaatact tcgttgtact
    10381 ttctttattc tacaatgttt ataaatgaaa ggaaagaata agtgatgtgt tcgtcttatc
    10441 tttctcgtta tataaaaaag gaacgtgaca aaagacgtgt cacatccccc ttattccgaa
    10501 taaacgtttt tatcgcaacc actgatacac ggcgtttcat cacattatga atgtcttctt
    10561 ctttggctac gactataaac cactggttct tcctgcgact gtgccttttt tgcaatcaat
    10621 ttcagacgat aaggctcatg atagcgtaac ccgtctttga caaaatcacc gccgacgatg
    10681 aacaatccga caggttccaa tgtgtcttct gttgagtcaa gtcgaatggt ccctactttt
    10741 tccatgtccg agtagcttcg tccaatattt agaacatact ctttctctcc tgttttcttg
    10801 acatagtaat aaggatacaa tgtatttcta tcgaccagaa atccatccat gcgaccgagt
    10861 tcatttgttt tgatctcacc atttccaatc aaataccgca tattctcggc taatttcaat
    10921 tgataatctt cgtcataaga aaccatgacc tcttgtgtat tcactttatc gatcgaattt
    10981 ccattatcta tcgattcttc tgtctggatg ttgaacgctt catcaggtaa atacttcaca
    11041 aaaagctgag tgacatcggc tttcgtgttt ttatctaaaa agtaataaaa tacatttaaa
    11101 acagtaaaat aaacaagcac tagaaatgca agtccatata agaaaaattg aagatagttt
    11161 tggttttgcc aaagtccata aacaattctc aataaataaa gcgtgggaat cacacttaaa
    11221 atcgtatatc cacggttaag atatttagga ctgatgtcta aatagccaag gaatttatgc
    11281 accccgttga taaggtctag taaaaatgtc attgagaatc acctgcttgc tggttatacg
    11341 tttgggtatt tcctgaatct tggttttgct gtgctgtacg tgctgcatca tcattttgcg
    11401 tgttatcatc tacagtcgta ctgcttggta tcgtttcgga gctagcactt gtatccggac
    11461 ttgtacttgg attggttgaa tccgatggag tgccatctgg taccgtatta cttgtttcgc
    11521 ttgagctgtc agttgtatat gtctcatcat acgacgaact agttgttgta tcgtcccatg
    11581 tacttgtcgt agtggtttca ttcaaacttt ccgttgttgt tggttcagat gtcgagactg
    11641 aagtagttgg tgttgtttct tctacagtac tagaacttga acttggtgca cggtagctgg
    11701 aactattcac aatacctgta gtagtcgcga caatcgtcga gattgttaat acagcgattg
    11761 gcttcaaaat aggtgctact tttcgcatct tatctcttcc ttcattgaaa tttattcttg
    11821 tttccttact ttactacgga caaattatac agtctacctt atttctacca taaattcttt
    11881 atttttttct tacgtttttc cgaaagattt ctttaatttt cctcaaaaac tacggttgat
    11941 gtttttaagt aagcttttgc ttcccgattg atttctttca gatcgcatcc ttgctgcttg
    12001 gcagcgaaca cgcaaccaca ataacattgg cgataaatat catactcttt gcacatttgc
    12061 aaagagcgtt catagccttt gtttttcttg aaatcacttg gcaagtattg tgtcccgtaa
    12121 atcttttgga tatccattcc gatctgattg atcaaagctg catttttctt tggcgagatg
    12181 gtcagggcac ttccaaagta atcgtatcct agttcttgcg ctttttgcgc aacaagatct
    12241 aatcgcatat tgaagcatgc actgcagcga gcaccacctt ctttttcgtt ttcaagatgg
    12301 tttgttaaaa ccattttggt gaattcattt ggacggtatt catcaacgat tagcgcaaca
    12361 tgatggtcag ttttctcatt aaattggtct acaaatcgtt tttgttcatg taatcttcgc
    12421 aaatactcac ttttaggatg aatattcgaa tttgaaaaaa ataacgtgat ctctgcatat
    12481 tgagacatga attccaatgt gtgcgtactg caaggagcac agcatgtatg tattaatatc
    12541 ttaggcttta cttcttcttt ctcccattgc atgatcattt ttttcagaac tgtatcataa
    12601 tttatttttt gatttttcat tttttctacg atttgttgag cattaatcaa tttgttattc
    12661 ctcctccttc ttttttctag gattttcctt attataagcc atttctattt taatcgaaag
    12721 ctgtattgtt atctgtttga taatttcttt gagtattcct aattcccctt gtatactggt
    12781 agaaacgact attgtcagga ggatagtact gtgcatgttg atttattctt tcgaaggctg
    12841 cagcggcgag gacagaagtt tgctgccaat catttctcat cgatccaaat catcgttttt
    12901 tattatatat tgatgacggt cttatcactc gttctatttt atatgccgat ttttcgcgaa
    12961 ccagatagcc atgtttcttt tgtggacatg ctgttcatgg caatcagtac agtgagtgtg
    13021 actggactat cgacttttga tatcaattct gtgttcaatg ataacggtat tatactgctg
    13081 gaaatattat ttcaagttgg tggtttgggg atcatgatga tttctactgc ttttgtcatt
    13141 ttttctaaac gacggatcac actgaaacag cgacagctga tcatgacgga tatgaatcaa
    13201 ccgcgtttgt ctggtatcgt ccgactgatt cggatcactt ttgctatttt aatctggttt
    13261 caattacttt ttgggacatt cttttctatt tatttttatt acagaggcta ttttgatcgt
    13321 tggcgtgatg ccgtttttta cggtttttat caggcgatat cggctgtcac gaactctggg
    13381 tttgacgtca ctggggattc catcaagccc tttgcacatg actatttttt cttgatcctg
    13441 atcatgtttt tgatttttgt cggaggaatt ggttttcctg tattgatgga gtgccgggaa
    13501 tggctattat acaaacgttc caatgcgaag cttccgtttc gtttttcctt gtttaccaaa
    13561 ttagctgtgt tagcttttgt tatcttgttt gtttcgggaa ctgtcttgat ttatctattg
    13621 gaaaaggacc atttattcca agattccaat atgagtgtca aatggatcaa ttcgatgttt
    13681 tattcgatta cgactcgtaa tgctggtctg cagattcacg atttgggtga ttttcaaatc
    13741 acaacactga tcattttttc tttactgatg tttatcggat gcagtcctag ttctgtaggc
    13801 ggcgggattc gaacaactac tgtagcaatc atcggtttgt atctatattc cttcctcaaa
    13861 agtgaagata acatcaatat ttttggacgg agaatcgatc aagatgatgt gcgaaaatca
    13921 gtcgttgtct ttatgctttc tcttggtatg tgtttctttt gtatcgtttt tttatctgct
    13981 acagaagaac aaacgctgat ctcgatcatc atcgaagtaa cttctgcgtt tggaacaact
    14041 ggtttatctt tagggattac cggagacttg tctgttgtgg gcaaaatcac gattgctaca
    14101 ctgatgttta ttgggcggat cggaatgcta tatacattga tgttattcgt gccaaaagaa
    14161 acacgagatc taggctatga atatccaagc gagaaaatta ttatcggtta aaatgggaat
    14221 ataggtaaga gggaataata aaatcacagc agcttgaaac aatcattttg tttcaagctg
    14281 tttttttgtt aaatcatgcg attttgtccg atttcatgca ctttcagccg taaccttttc
    14341 ttaagaataa gaaaagcaat ttaacctttt tttcaacata tggtatgatt agttgaattt
    14401 attcgtcagc aaatcgtttt tggaggttac ataatgggaa tacgcagtca ttttgcgatt
    14461 gcagttggaa aaacgtcgca atgggcatta aaaacttttt ttaaaggcgg atcaagttta
    14521 ccaggtaaac tagcgctgaa agtcgatcca catatactag atagtttggc aaaagactac
    14581 caagtcgtcg ttgtcaccgg aacaaacggt aaaacattga ctaccgcttt aacagtcaat
    14641 attttgcgcc agcaattcga tgaagttcta accaacccta ctggtgcgaa tatggtacag
    14701 ggaattgtat cgactttctt gcaggcaaaa ccaaaaaaag gacagaaaaa gtttgctgtc
    14761 ctagaaatag atgaagcaag tttgagcaaa gtcacagaat atatgaaacc agaactgttc
    14821 ttatttacaa atattttccg tgatcaaatg gatcggtatg gagaaatcta tacgacctat
    14881 aaaatgattg tagacggtgc cgcaaagtca ccaaatgcaa cgattatcag taacggtgat
    14941 tcaccaatct tcaattctat cgaaacagtc aatcctagaa aatattatgg ctttgaccat
    15001 gaagaagatc gtgaacaaat ggctcattac aatacagacg gtgtcttatg tccaaattgt
    15061 catcacatct tgcattataa aatgatcact tatgcaaact tagggaaata ttactgtcca
    15121 aattgtgact tcaaacgtcc tgaattggat tatcgattaa ctgatatggt acggatggac
    15181 aatgtgtcag cagatttcgt gatcgatggt catgaatacg gaatcgaagt tggcggtatg
    15241 tataatgtct acaatgcttt agcagctact gcagtggcag agtacttcaa tgtcgatcct
    15301 gataaaatca aacaaggttt aggatatgac gagaaagtat tcggccgaca agaagtcatc
    15361 aatatcaatg ggaaaaaatg tacattagta cttgtcaaaa atcctgtagg tatcaatcaa
    15421 gtaatcgata tgattggtct agctccttat ccattttctt tagtggcttt attaaatgcc
    15481 aattacgcag acggaatcga tgtcagctgg atttgggatg gtaattttga aaactttgcc
    15541 gatatggata tcccagctgt tatttctgga ggagaccgtc atacagatat gactttacgt
    15601 ctaaaagttg ccgggattcc agaaaataaa ttgacagaaa caaaagaatt accagaagta
    15661 atcgaaaaaa tcaaagaatt gccaactgag catgtctata ttttagctac ttacactgct
    15721 gtattgcagc ttcgtaaaga actagctgct caaggattta tcaaaggagg aatgaaccgt
    15781 ggctaattat gaactcaata tcgcccacct ttatggaaat ttgatgaaca catatggtga
    15841 taacggaaac cttttgatgt tgaaatatat agcagaaaaa atgggggttt cttgccatac
    15901 ggagatcgtt agtatacatg aaccttttga tgctgataaa tatgacttgg ttttctttgg
    15961 cggtggtcaa gactttgagc aattaatcat ttcaaaagac atccagaaaa agaaagattc
    16021 tttgatcaac tatatcgaaa atgatggcgt catgttagcc atttgtggtg gctaccaatt
    16081 gctaggtcac tactacatgg gagctaatgg agaaaagatc caaggaatca gtgcactgga
    16141 tcactacacg ttaagtcagg aaaataaccg gtttatcggt gatatcgtta tccataacga
    16201 agaattcaat gaaacttatt atggatttga aaaccacaat ggtcggactt tcttaggtga
    16261 aggtgaacga ccttttggga aagtggtaca aggtcaagga aataatggtg aagaccaatc
    16321 tgaaggtgtc atttaccgaa atgtcttcgg ttcttatttc catggaccta tccttgcaag
    16381 aaatgagaac ctggcagctc gtttgatccg cctagcttta gaacaacgtt acggtaaaga
    16441 attggatctg ccctatcttg aaggacaaac agcctagtta ttcgaaaaag gggaatgtga
    16501 caaaaatttc gtcacattcc cctttttcgt atcctatttt aagcgacttg cttggcataa
    16561 cggttcacgt tttgcttgat attcccgatt gttttcggat cattgattcc tgtgacttct
    16621 tgaatcaatt gttccaagtt ttcatggatc ttcatttcat gcaattgacg gctttgttca
    16681 tcttcaggat catagtaatt aaaaataatc cccattgttg ccgtcaaatg tgggcaagtc
    16741 aagttatgtt cttgtagttc tctcactgga cgtgtaaatc tttcttgata acctaactta
    16801 cgtaatggtg tccgagcgac acgtgtgatc gcatcagaga tatttttgtt ttggaagcga
    16861 tgaatgattt tttcaatata cgcatgatgc tgctctgcat caaaccccca tttggcaatc
    16921 agcaaacttc ctgtttcctg caaaactgat tttaattgtg ccacaactaa cgcatcttgc
    16981 attgcttcat caatggtctg gtaacctaac aaggcacctg tataagcgac agtcgcatga
    17041 ccagtattga cactaaatag tttgcgttcg atatatggtt ctagatcatc tacatataaa
    17101 acccctttta attggatttt tttatttttt ctttggctat cgtcaatgac ccattctttg
    17161 aaaggttcca cttggacgaa taaaggatct tcatggtgtt gtaatgggac gattcgatcg
    17221 actgctgcat ttggaaagcc gatatatttg tcagtaaatg atgtttccgg aagatattta
    17281 tacacttctt tttctagaaa tgctgaaccg ccaatcatat tttcacaggc aatgatatct
    17341 aaagatgttg tgcttccttc cttttctctt tcttggatac cttttgcaat caactcagca
    17401 ataaaaggta agatattcgg gccaatggcg gtagtaacta aatctgcttc agcgatttct
    17461 tggattactt tttctggatt tgtttgattg ttgattcctt tgacattttt tacagtgatt
    17521 ttttcttttg attcatctgc taattcgatt tcgtattctt ttctttcatt taaagcatca
    17581 atgatcgtgt gattcacatc tacgaaagta atctcaaatc cattagctgc caaaacctct
    17641 ccaataaatc ctcgaccgat atttcctgca ccaaaatgta ctgctctcat ttttctctct
    17701 ccttaagcaa ttgctagatt ttccgttact tcttctttgc tgagcgcgtc tgctagctgg
    17761 acaacattgt ccatatcact gcaataaagt gcgatttgct gtacaagttg caaatgctca
    17821 tctccgactc ctgcaatacc gaacaaaaca gtcgcaatct tttcttcttc ttcggtacca
    17881 aaatctacac catctggtac ttgaacgaca cagatgccgg attttttcac atattttttt
    17941 gcttcatctg ttccatgagg aatcgcgata aaattcccca tataaacaga taacattttg
    18001 tctctatcta ccatggcatc gatatattcc tcatcaacgc accctgcttc tacaagcatc
    18061 tgaccgcaat agcggatcgc ttcttctttg gttttaaaag atcgattgag ctcgatcatg
    18121 tcgaatggca aactttgcat gctgttttcc tcccttaagc tgtctgtttc aaatattgtg
    18181 caatcgattc gtatttttgc ggattgacca tgctgttgac tgataaatga atggattgag
    18241 gtgcagccat ctgtactttt tccgctaaac tttctttggc tacgatcagt gaatcaggtt
    18301 catcttttaa atcttggata ttgacttttc ttaacgggat agttaaatgg ttcttgtcta
    18361 atatatcttt gagcacggtc atccccattg ttgctgatcc ggaacgatca tcgtggacaa
    18421 gtagaatctc tttgatttgt ttgatgtttg ctgaaagaaa ctcttctgct ggctcattct
    18481 caatttcaga aactgatgtt ttctgcttgc tgcttaattc ttgaatgatt tcatcatatc
    18541 gaggtgaatt caaaaagttt tctactgaga caaaacgcgc gctcggtgct ttttgttttg
    18601 ctctttcttg tagttcgacc tgagtaacga tcaatgtatt cgagtcatct gtcagttggc
    18661 tgattgcttg attggtcacc gtttgaggca agtctgcttc ctgtactttt tttctcaaga
    18721 tcgaagcgcc catcgcactt gatcccattc ctgcatcaca agcaaaaatg attttttgta
    18781 cgccagagaa tgtagctcct tgaggaatac cttgggcacc tttggaagct tgcttagctg
    18841 cctttgtctc ttgaacagat ttttcgaagt cttcatttgt atctttcgca tccgctttca
    18901 aaattactgc agaaatacca aacgaaacag ctgcggcaac aatcacgccc aaaataacag
    18961 gaaggtaaga attgaacggt gtcatcgcta gaatcgcaat gatcgaccct ggagaagctg
    19021 gcgctcttag tccagcatcc atcaattgga aaatgaaact tcctgacatt cctccgccta
    19081 taactgataa gaataataac ggtttcatca taacgtaagg aaaatagatt tcatggatcc
    19141 cgcctaaaaa gtgaatgatc atggctcctg gtgctgaaga ttttgctgct cctttgccaa
    19201 acaaagtaaa agcaagtaat acgccaagtc ctggaccagg atttgcttca agcaagaata
    19261 gaatagattt tccagcctct gctacttgtt cagttcccaa tggtgtcaaa atcccatgat
    19321 tgattgcatt attcaaaaat aagatttttg ctggttcaat aaaaatactt gtcaatggaa
    19381 tcaaatgggc agataaaatg atctctacac cttttgccat tgcttgagtc aatgcatcga
    19441 caactggacc aatcgcagaa aaacctaata acgctaatcc aaaaccgata agacctgctg
    19501 aaaaattatt gactagcatt tcaaaaccgg atttgatccg tttttggaat agctgatcaa
    19561 atttcttgat ggcatatcca ccaagaggtc ccatgatcat cgcgcctagc atcattggaa
    19621 tatctgttcc gacaatcaca cccatcgttg caattgcccc aacaactgat ccgcggtcac
    19681 cagcaattac ttttccgcca gtataaccga tcaataaagg aatcagataa gttagcattg
    19741 ggcttaccat ggtagcgaaa gcctcatttg gtaaataacc atctgggata aatagagccg
    19801 ttaatacacc ccaggcaatc agtgctccaa tatttggcat gaccatgctt gataaagtac
    19861 tgcctaattt ttgtaccctc gcttttagcg aaatgttgtt tttgggaatc gtccgttcca
    19921 ttttgtctct ccctctctcc ttttgatact ctaattgtat ccgcttacta aaaagagaaa
    19981 tagtcgtact ttggcacgaa actttgtgcc aaaggtttga gtatgtcagc aaaataaaaa
    20041 gatttcggta tcagaaaact tcaattttaa ataaaactgt tattttaatt tttttataat
    20101 atgcaaacaa taacttttgt tagcatttaa tttagatgtt ttccattcta ttatgttatt
    20161 aaattttata ttggaggcct atctatgaca ttactgatca aaaacatgaa catgaaacgt
    20221 ggtaaaaaac gtgtgattcg acaattgaat cttttagtag aatcaggaga agtagctgct
    20281 ttgattgccc cgaacggatt tggcaaaaca actttgctga atggcattgc ccaactgctg
    20341 ccaacaactt atgatcaatt gtcccttcac aacatctctc ctactgctta tacagattat
    20401 cgccggtctt tcttttttct agaatcaacg aatcagttat ccgactatct atcggctgtt
    20461 gattatttga ctttgatcaa atactattgg cagagcaaag tagaatgcca aactgttttc
    20521 caacgattga agatgactga gtaccttact ttgccgattg gaaaaatgtc tctaggaatg
    20581 aaacaacatg tcttgcttgc tgcttacttg atcagtgatt catctgtcat gttattcgat
    20641 gaaccgttga acggattaga cccaggaagt attgatatct tgaatagcct catcagacaa
    20701 tggcagaaag aggggaagac gattcttctt tcttcccacg ctatcgcgaa tatccaatcg
    20761 atttgttcta aagcatgttt tctaaaaaat ggagaaatcg tcacagaatc ctcaaatatg
    20821 gaagagattt tagctattta tcgtaaatta tatgaggtgt aactatgtct ttacttacat
    20881 ttgaacgcaa tctcttacta aaaaacaaag taaacttttt atttcctcta ttgttggtcg
    20941 tgctctttgc attcccactt ttttttgatc ataaacttgc ttatacagaa ttcgatgaac
    21001 tgaatcataa ctatgaagag atgcaacgtt tgattgaaac gttgaaagaa gatgagaatg
    21061 agaaagaatt tgtggaaagt ttagagaaaa gcaacaaact gatcgaagca atcttacatg
    21121 caaaaaacac agggaatgtc cagcaaatgg tcgaagcaac ttatcattat gaaaaagata
    21181 tactggatcg actgatttct ggtcaaagac aaggaattcc aatcattgaa caacaaaaac
    21241 gtgtcgagtt gctccgttat atgaaagaac atcagatcca aagatattct atttttgact
    21301 tgccagcgca tctctcttta gcaaattact acgaaaatat tttttcagga atgatttcta
    21361 gttttttgat tttatgtata accgccttgt ttctttcttc catcatctct tatgaaaaaa
    21421 gaaagcaggt aatctctcta gtcaatctat tactggatag tatggtaaag aaacatagca
    21481 tccgttttac catttattat ggtgcagcta tgctaagtct cgtgatgcca tttttgatcg
    21541 tctctatact agtaataatc aaaaatggtt tgggagactt ccgttatcct gtaggaacga
    21601 tcattggtca ggagatacga atcctgccga tgtatgagta tcttttccag agctttctgt
    21661 tcctgttgtt gtgggtgctc tttttaagta caatcagttt tcttctatct gctttatttg
    21721 aacattctct agtcaatctt ttaggtacac ttctttgtct tttcttagca gagtatcgct
    21781 tgttttcttc gattggatgg atagaatcaa tcagtcatta cttgccaact tcttatgttg
    21841 attttcaaaa tgtgatcata ggaggagata ttttctcgcc actagcttca gaacaggtta
    21901 ctttcatgaa tggtatcttg acacttggta tatggagtat cgttttatta ttcataggta
    21961 tgggcactat ctatataaaa aagagctatt aaaacacaaa aataataggg gcggtgacaa
    22021 aaaccttgtc accgtcccct atttcaaata aacggttttc tagaataaat agttttcaaa
    22081 agcttgcatc cgcagtaata agaacctccc aacaaaaata gctcctctta tttttatctt
    22141 ggttgaagtt attacttgat gcaaggcagc tttttttact gtgaacattg gttatatatg
    22201 tccatgttca aaaatcattt ggacttcttc aatggtttcc gcatcactga gcgccattac
    22261 ttggtcgatg tctgaacaga aaaaggctaa atcttggagc accgctaact gatgttcttt
    22321 ttttaaagcc actacaaata agagtgtcgc gatttttggt tcctcttttg ttccaaagtc
    22381 cacaccatca ggcacttgga ctaaatagat tccttcttct actacttctt catctttgtc
    22441 agtcccatgt ggcaaggcta cgaaattccc gatatagaca ctcgcttttt cttgtctttc
    22501 gatcattgat tggatatatg ccggtttgat tcccccttgt tgggtcatcc actggccgat
    22561 ctcggtcaac gcttctttcc aattttgata ggattgatcg agcaatattt tttctgctgg
    22621 tagctgtatc atcttttcac cttcatttct ttatttcttc aatcaggatt ttcgctaaca
    22681 actgataaac gatttcttta tttcctgagc ggaagatctc ggtgttcaag tcattcatga
    22741 tgattgctcc actgatcttt cctaaaagaa tcccgtcttt ttcttggatt ggcgaaggcg
    22801 ccagcataac taacatacgg gacaaatcga ctggctgtct atccatccct tcaatggtca
    22861 acggatgttt caaattgaaa atacaaaaga tcggtttaga aatcaatgag ctggatgtat
    22921 ggaataaccc catatttgta tgaggaatac cgataggtgc ttgttcatat cgcttaagca
    22981 gccggcgatg aaccacctct tcatccgtta ctagtccttc aggaaaataa ggaaataagc
    23041 tgcgaatcgt ttcggaaatc gtctcaggat tatccagttc ttggataaaa aaacgaccga
    23101 gtaattgatt gatttgatcc atgaaatgca ttgtttcctc ataagtatct tctatcatcc
    23161 actcgttatt gctgttatac gtaatttttt gccttttttt cttctcaata tgcgaaaaag
    23221 cttcttttag ctgcttgact tcatcttcta gcagtagcgg ggagactaaa atatattttc
    23281 caccgtatcc tggtaaaata gatgtagaaa taacaacatc atattcttct tgtaaatcga
    23341 ttttttttaa atcagcaatc cgatagaaat gaatttgttg gatgaaggga aaataccttt
    23401 tcaatcgtag ttccaacatg ctagtagaag ctaatccact tggcgaaaat cctgcaatac
    23461 tgatattgct ttctttcgga cttcgttcca atgaattcgc aaaatgaagg accatataag
    23521 cgatctcttc ttcggataat tttttttctt gaaaaatccg ctctactgct ttacggattg
    23581 cattcgcaac ttcctcgtat tgaaccatga ttttttctaa aatcggattg gtcagtgtct
    23641 cttcttgtaa aacggctctt gaaaacacac cagataaatg agtcaacagc attttgtaca
    23701 aagtttcatc ttcataaaaa tggacattcg tctcttcact tacttcttga atcagttgtt
    23761 ttactaaata agcaagctgt gtatcgaacg tctcttcaaa gaaattctga tcaaatgagt
    23821 tcgtgaagtc atttaataat cctgcaaaaa agacgatctc atttactgga taaagttgtt
    23881 ttgtctcaga agcgatccgt gaaaacaatc gtttagctgt ctgtagtaat tctttgtttg
    23941 ccagactatt atatgtctca tttgtcagta aatgattttt cgtcacacgc tccatcgtca
    24001 atgtcagcat caaaaccaaa tgttcaagct ttctatcact tagattggtc tttttttgga
    24061 tcaattcttc cccgattact ttgcgtgcaa gagatagatg ttgcggatca acgaatcgaa
    24121 aaaaataatt cagtgatgca tcgaaagaaa tactatgaaa aagttcatat tcattgattt
    24181 ctaattctaa tacattcgct gtcagaagcc ggcggtattt ttccggtcct tcgatctcgt
    24241 atccctggtt ccgaatgatt tccaaaggca gttggcgaat actttcttcc aactgtttga
    24301 tatctgcata aaaagtggta ttactgatgc gatatgattc catgaaatga gcaagactca
    24361 acggctctgt cgtcagtaac aactcggcta aaatcgcgtg ttgtcgttta gcagtggata
    24421 agtcattact tttcccatct gtattttctt ttaattgctc taatacttct tctgatgcct
    24481 caatgacaaa tgagcctcta gctgcttttt tcaaagaagc atttaccgat ttcaatgaga
    24541 cttcaagatt atctaattcg cggtaaatag tccgtttact gaccttcaat aaattcatca
    24601 ttttttggat agatacaggt gacgggctac tgagaaattc tgctaataat tgtttttctc
    24661 tgggcgataa atacatctta tatttctccc ttcacgagtt tttcgtattc attcgtagct
    24721 acccaatgtt caactagaac tagccgaggg atcttttctt ctagctgttc agttacagct
    24781 agttctcttt gtgcgatata aatcgtttct tcttggattt tgattttttc tattgattct
    24841 tttgagataa caaactgttt tttcgctttt tttgctaatt gacttaaaat cgtcatgccc
    24901 atagtttgtg agccgcgtct gttgttttta tacaaaaata caatattagc aggcgacgtt
    24961 ttttgttctg tgatatctgc tatctctttt ttttcttccc gctttggaaa ttggatcgct
    25021 tcttttattc gtgacagcaa gatatctgtt tccaaaaagt gatccagtac atagatttga
    25081 ctttgaggta ctttttctct tgcaagaccc gctagttcat gttgcacgat aaccagctga
    25141 tttacttggt tattcaattc atagatagag ctgtatccga cagaaagatc gattttttct
    25201 gctgctaatt tctttttcag cagggcggct cccatagcac ttgatcccat acctgagtcg
    25261 caagcaaaaa tgacggaatg aatgatagat ggttccaaga tttctcccct ttctttgacc
    25321 gttttgttct ctttttcttt atttggtaat ttcaatagta tcgctgcaca aatgaaggtc
    25381 acaaatgtac tgattgctat ccctatactt accccagcta gttgtggata aggcgcattg
    25441 gcaaaaatca cgagtagtga cccaggcgat acaggtgcag ctaatcccgt atctaaccat
    25501 tgaaatatca acgtgccgct tgccccacca gccattacag ctaaaaatag atacggtcgc
    25561 agcaagacaa atggaaaata gatttcgtga atcccgccaa tcgcatggat catcaatgct
    25621 ccactcgcat ttactttttc ctgtttttct gcaaaacaaa ggatagaaag taagatccct
    25681 aaaccaggtc caggatttgc ctccatcaaa aataaggaag agtacccgac tgtttgtgag
    25741 aattcgattc ctaaaggtgt caatacacca tgattgattg cattattgaa aaatagtact
    25801 tttagaggtt ctaagatcag actgacaagt ggtaacagat tcattgtgac tagccaagac
    25861 acaacggagg cgagcaacaa gtgaacacta tttaaaactg gttcaataaa aacgattcca
    25921 attatacaaa taacgctacc agtcagtcct actagtaggt tgctgacaag catttcatat
    25981 ccagccttga atttcacatg gaatctagaa acaaattgtt catatacgat gccggcaagc
    26041 ggcccgataa tcatcgctcc tacgatttgt ggagcattgg aacgagcgat caaccctaaa
    26101 gaagcaattg caccggcgac tgcccctttt tgtcccccta ccgatttccc gcctgaataa
    26161 ctgatcaaga tgggtaaaag cagctggatc atccaatggc atacctcttc taatgtggtg
    26221 tgcaaatcgc cagacacacc ttttgttgct aatgacaaga ttccccaagc gataaacaga
    26281 cttagatttg gcatgatcat ttcatttaag tgatgtccag cttttctcag ttgtttcata
    26341 gacacgcgac tgctgttgtt tgctttccat ttgtattcca tctttttcac cagacttcta
    26401 tttattgatg gacggatttt atgtagaaaa agcagcaaac gaaaaaccgt ctgctgcttt
    26461 cagctatctg ggaactattt gcctggttca gacgcaatga ttgctagttc accttggacc
    26521 gcccactcag aaacgccatt tggaataata cagcttgttc ctttttctaa tggatatgtt
    26581 tttccgtcaa cgacaaggtc tcctgctcct tcgattaccg tcatcaatgt ataaggggct
    26641 tgttttttga aagatgtgat tcctttaata tcccactcat atacattgaa aaactcagtt
    26701 tctaaataag tgacgatcga tgaattccct tttcggactt ctttgatttg taattctggt
    26761 gttttcgcag ggacagttgt tacatcgatc gattgttgga tatgcagctc tcttgtttgt
    26821 ccttgcgcat cttttctgtc gtagtcatac acgcggtaag tcgtgtcaga actttgctgt
    26881 gtttctaaaa tcatgattcc tttaccaata gcatggatcg ttccgcttgg tacataaaag
    26941 aaatcacctt ttttaactgg tactttcttc aaaaggtcat cccagcgccc atctttgatc
    27001 atctcagcta gttcttctct tgtctttgca tgatgaccat agatgatttc tgcaccaggt
    27061 tcagcatcga tgatatacca gcattcagtt tttcctaatt caccttcatg tttcatccca
    27121 taggcatcat ctggatgtac ttgtacagat agatcatctt ctgcgtctaa gatcttgatc
    27181 aataatggaa acactggttc tgaaggatgg ccaaacaatt ccttgtgttc tgaccatagt
    27241 tcatctaatc ttttcccttt gaattcgcca ttttctacta cactgacacc atgaggatgc
    27301 gcactgatcg cccaatcttc tccgatttta tcgttaggga tatcaaaacc aaagactgaa
    27361 cgtaaacgac ttcctcccca aattttttct tgaaaaactg gttttaaaaa cataggttcc
    27421 tgcataattc aaactcctct catttttcat agctattata gcattatttc ggtaataccg
    27481 ctttctcttt caaaaagttt tcaacaattt catctcgaca tatgagatac tccgcacgat
    27541 gatcgacagg atgtacgcga tttgacaaaa atatcaacgc ttcttgtgtt tttatatcaa
    27601 tcaagaaaaa cgtacctgta taaccagtat gaaaaagaat cgcatgaccg tcttctacat
    27661 ccttttttaa gtcccatcct aaagaacgat agccattttt tttcggtgtt tgatcttgca
    27721 gtaaagaatc aatcgtctct tcttctaaaa aacgaccatt acggattttt cccttgttca
    27781 aatacatttg gacaaaagta atgctgtctt tcaaatcaat aaagagaccc gcgttacctg
    27841 catgttcagc caaaactctt gctttcggat catgagtttg tccacgcaaa acctcacccg
    27901 aaggcagttg ttctgtggga acgatttttt tggaaggaaa aggaagaaat aagctgttca
    27961 tcattcctaa tggttccaat acttgttctt gaaagatttc cactgcagtt tcttggtaaa
    28021 tttcctctag catcaaccca agaagaatcg taccggcatc tgtatatttt acttcttttc
    28081 ctagttgctc acctggttct agcttcaaat aagcggcgat caattcttcc tgagatagct
    28141 tatctctgtt tggtatccat gttttgatat cagaagtatg tgtcagtaaa tgacgcaaag
    28201 tgattgtctg attttcaaat tcaggaagat atttatgaag cggttgatcg agttctatct
    28261 ttccatcttc taatagacgt aaaactaatg aagtggttgc aacgactttc gtcagtgagg
    28321 ctgcatcaaa caacagatct tctgtcatca gctgcttttc aggaacgact tgggcaaagc
    28381 cggctgtaaa tgtttctact tgattttctt ttatgaaggc gactgctaca cctggataaa
    28441 atccttcttc catctttttc aaaataagct gtttcgtgtg tggatacatc tttttctcct
    28501 gcttttctat cagattctta cagtccaagc ttaaagctta atctatgagg aatcaacctt
    28561 ttttacacaa aaaaactgcg cgaaaaaccg cgcagtttcc cccggtatac tattttctct
    28621 ttctatctct agaataagcg atagtatgac cgttcataaa atgacgagac atgcgacaat
    28681 cgttaagaaa gaaaaagtta agcgtcgcat cgaatgtcag ttttattctt ttcttttggc
    28741 tcactgacga accaccattc gattcctata gcatcataga ttgtgagtac tgtgtgcttt
    28801 ttgtctaaga agaaatcaac tgatagttgc tgcaagtgag caatcagtcc ttccatgctt
    28861 tcagtatcga tcatgaattt cagaaaatca agtccaatca cttcatctgt aggcaaatca
    28921 attgcgcctc cgctagcttc tgtcaaacca acatggaaat ctccttcatt caatacatgg
    28981 atgccctctt cttcattatg taccttcaaa ccaagtacat gattaagaaa ttggcgttct
    29041 tttgttaagt tagtaacatt taaatggatt ttttcaaaat aagaacctgt tgatagttta
    29101 ctgaattttt cttcagattt tcttaataat gcttcttgat ccattgtttc tggaatttct
    29161 ctttggtcgt tcttattctc ttcatggtaa atttccaatt cattctcttc tgggtctacg
    29221 agcaaaatcc ccatacgtcc atgatcttct aaagcttctt tgattggata attcatttta
    29281 cggatacgac aaaggatatc tgccatttct tctgtgctag ggataatcaa tgaaaaacgg
    29341 ttcagctttt tgatcacccc catgtgatca ttcgctctag gactttcttc cagcagcaac
    29401 atttcacttc cagcttcttc tgttcctaaa atggctaatt cgttttcttc tcttttcaaa
    29461 tcaaagccaa caacgttctt gtaaaaatca atcattttat ctcggtcttt cacccgaatg
    29521 gctacacttt ttaaataggt ggcagccccc aattgaaaat ctttcatatt tcttgctccc
    29581 ctctacgtca agctttattt taacgatgga ttcttcttct ttcaagcaat aactttgatt
    29641 ttgttaaaaa aaatgtgaga aaattcgcat tttgcttgtt ttttgtaaat taatgagtaa
    29701 aaactcttat aaaaagaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    29761 aacaaaaata gctcctccta tttttcgttt ggttgggctt attaccgcag atgcaagctt
    29821 ttgaacaccg tttaaggaag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa
    29881 attttgtgaa aatggctttc tacatttttg cataaatttg gcttaatacc gagaaactgg
    29941 cttttgaaca ccgtttacca agaggttgtg agcctgccgt ttaactccga gtcacaagga
    30001 gcaatttcaa aaaacagctt ctcaggtttg ttgactattg tgacttgtga ccgaggagtt
    30061 ggcaggagaa caccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag
    30121 agatacaaaa cggaaaaata gcttctcata ttttgccgtt ttttgagcta ataccgaagg
    30181 acagctgatt gaacaccgtt tattcggaaa aagggactgt gacacgactt ttgtcacagc
    30241 ccctaaaaaa aataataaat ttgtactatt ctatgaagct tcttcttcag aaaactgact
    30301 gttataaaga tctgcataga atccgtcttt ttccatcaat gtatcgtggt ttcctgtttc
    30361 tacgatcgaa ccatgattca tcacaataat gttgtctgca tcgcgtattg tagacaagcg
    30421 gtgagcaaca acgaaacttg tccggttttc caataggcgg ttcatcgctt tttgaatcag
    30481 tatttctgtt cgtgtatcta cactagatgt tgcttcatca aggatcagga catctggatt
    30541 tgctaagaat gcccgcgcaa ttgtgattag ctgacgctgc ccttgcgaaa tattgcttgc
    30601 atcctcgttt aatacagtgt tatatccttc tggcaattta cgaacaaaat cgtctacgtg
    30661 agcagcttta gcagcctcaa tgatttgttc gtctgttgca tcgtcgttcc catatttgat
    30721 attgtcatag atacttcctg taaacagcca agtatcttgc agtaccattg aaaactgtgc
    30781 gcgcaattca tcacgtgaaa gatcacgggt atctacacca tcgtaacgaa tactacctcc
    30841 agaaacatcg tagaaccgtt caagaaggtt gatcagtgtc gtcttccctg caccagtcgg
    30901 acctacaata gcgaccatct caccaggttt gacatccaag ttaaaatcag tcattagcaa
    30961 gttatctggt gtgtagccga attgaacatt ttcaaaacga actttgtatg gtgtatcttt
    31021 ttcttctgga aggccagatt tttcattgga catctcttct tcatccaaca cttcaaatac
    31081 ccgttcggct gaagcaactg ttgattggat agtgttcatc aaacttgcaa gttgagtgat
    31141 tggttgagag aactgtgttg tatattgcaa gaacgcttga acatccccta atgtcatgtt
    31201 cccatttgca acttttactc cgcctagtac agcgacgaac acatagccta gatttttaac
    31261 gaaattcatc aacggcatga tgattgccga aataaattga gctttccaac cagcggcata
    31321 cagattctga ttttcttttt caaatacttc ctggtctttt tcagtatgat tgaatgtttt
    31381 aacaatggta tgaccaccat atgtttcttc tacttggtta ttcaataaac ctaaactttt
    31441 ttgctgatct gcgaaaaaca cttgtgattt cggcgctacg atcatcacga tgatcaagct
    31501 taaagggatc atcaataacg cgactaacgt caattgccag ctgattgaaa gcatcatcac
    31561 gatgacccca atcaatgtga ctgcactagt tacaaattga gtcaaatttt gctgcagcgt
    31621 gcttgcgatg ttatccatat cattgattgc tcgagacatg atatccccat ttgtatgtgt
    31681 atcataatag gaaatcggta cacgattcat tttggcttcc aaatcatgac gtaattcata
    31741 aactgttcgt tgggaaacac gtgtcataat aaattgctgt aaaaagttga atacagcaga
    31801 aatcaaatac atcacgatta ctgtcgcaat gatctgcgcg attttatcaa agtctatcgg
    31861 aaattttgtt acttgttggc cttgttgcat ctgttgagcg ccagccatta cacctttgaa
    31921 tatttctgtg gttgctttcc ccaagacctt tggtgtttgg atttggaaga tcacggctgc
    31981 tattgctagt acaaatacag cgatgatcgc aaccatacgt ttagacatat agccaaacaa
    32041 tcggcgtacc gttccccaga aatttttagg cttttcacct tttacaccga gatttctgcc
    32101 aggtccacca tgtcctggac cgcctgctgg tccatgagag ggacgtgtgt ttgttttact
    32161 catgctaagt cctcctctct tagttgtgaa tgaacgattt cttgataagt ttcgtttgtt
    32221 gctaatagtt cttcatgtgt tcctttgcca accagttttc cttcatctag cacaaggatc
    32281 atatctgcat ccattactgt acttactcgt tgagcaacta aaacggtgat actttctttc
    32341 atattcgttt tcaatgcttg gcgaagattg gcatccgttt tgaaatccaa cgctgagaag
    32401 gaatcatcaa acacatagat atctgctgtt ttgactaatg cccgtgcaat tgccagacgc
    32461 tgtctttgtc caccagagaa gtttcctcct ccttgttcca catggctatc taagccatct
    32521 tccatctctg aaacaaaatc tttagcttgt gcgatttcta atgctttcca aatcatttca
    32581 tctgttgcat ccggcttacc atactgcata ttgtctcgga tcgttccaga gaacagaact
    32641 gctttttgag gaacaaagcc cattaattct cgcaaattat attgagtcat gtcactaatg
    32701 tccgttccat ttatttggat cgaaccagat tcgatgtcat ataatcgagg gatcaaattg
    32761 actaaggtcg ttttacctga accagtcccc cctatgattg cgacgatctc gcccgacttg
    32821 gcttggaaat caatatcttc caatgcaagt ttttctgctc catgatatcg ataattgaca
    32881 tgattgaagg ccaatgttgc gttttttcca gcaaaagaaa ctgttttagg attttcaggg
    32941 tcatggatac cgattttttc atccaacact tcattaatac ggtcagctga agcttgtgca
    33001 cgaggcacca tgataaaaat agcggaaagc atcataaagc tgatcaagat ctgcatcgca
    33061 taagtcataa atgcaatcag attaccgact tccaaagtca tatctgctat gtagtgtcca
    33121 ccaaaccaag tgattgctac gtttgtaccg ctcatgatca atgtcatcat tggcatcatc
    33181 aatgcgacga tcgtattaac tttgattgcc gtatttgtat aatctttatt cgctaaatca
    33241 aaacgatttt cttcaaagcg tgtcttatcg aatgctcgaa tcactctgac ccctgtcaat
    33301 ccttctcgaa ataccaggtt taaacggtca gttttctttt gcatactttt gaataatgga
    33361 acagcaaagt acatgattcc gccgatcaaa ataatcatta ctgggatgac aactaagaag
    33421 atttttgtca actgatgatc tttttgataa gcaagtatgc tcgcgccaat caacgtgatt
    33481 ggtgcattga tcatcatacg taaaaacatt tgtgtaacca tttggatttg agtcacatca
    33541 ttggtcgttc ttgtgatcaa agaggccgtt ccatatttat caaacgcatc ttttgtcaga
    33601 ctctctgatt ttttatagac atccgtacgc aattgcttac ccagtttctg tgattcacga
    33661 gtagcaaaaa atgtatttgc gatagcagct aagatactga tcaaagaaaa gccgatcatc
    33721 acaaaacctg tatgccaaat ataatcgaca tctccttgcg ctaccccttt atcaataata
    33781 ttggaggtaa gtgtcggcaa atataaatct gccagtactt ggatcaccat gaaaactgca
    33841 gctgcaattg ctgaagtcaa ggagatccgt tttacgattt ttagcatcta gttcccttct
    33901 ttctatttat atactccgtt tttcagccaa gaaagtttca aattgaattc actgattgct
    33961 tgctctactg agtaatcttc agatgttgaa aataagcgat acgaatgcgc aattgttgta
    34021 aatacagtat tcatcactaa ttggatcagt aattccaatt catgatcatt ttttactttc
    34081 agcttcgtcc gatcgatcaa ttcaagcaac tcttttgcat cttgtttgtg ttctttatgt
    34141 ggatcgtgag gcgttttttg gcgactcgta tgaggagcga cttttcttga ggcacggtaa
    34201 tccatgttca taaacaagtt acgataaaaa gaagcattct cgccttccaa aatctcataa
    34261 atcattttag aaaaatagga ttcgaatcct ttgaatagat caccatcgga ttctttcatt
    34321 agttgtaaca tatctttaga gctgtcacgt ctcattgtct caaagtaata aaaataaaga
    34381 tcctctttat cttcaaaata ctgatagaaa ctgcctctag ggatatccgc taatttgacg
    34441 atattagcaa tcgaagcttc ttttaagggt actctagaaa actcgattct cgcagcctcg
    34501 attagtcttt tttgtttttc tttaggcaaa tggaaaaatg tctgtgtcgg catatggatt
    34561 ctcctttctt tcaagatgac acattgtcat atgacgctat gtcattataa cacctcctac
    34621 ctgttttgca agacatttgc atagtttttt tcattatcat ccgatacatt aaaaaagtga
    34681 taagaggaat ccttccattt gaattaatga gagttgccca ttaaaatagc cacatactaa
    34741 acaaaggatg agataaatgg cccaatcaat tagtgtccaa gacttcaata aattgcctct
    34801 cgatgctgac agcatcgttt tagatacaag aaatacctta gattacacaa aaaaccactt
    34861 aagccactcg ctttctatcc ccgctgctat cttgccaaga caattaaaaa atctagatcc
    34921 aaagaaactt tattatattc taagctatac aggcaggcga tcagaagcat tagccgatca
    34981 acttactgct aatggttttc gtgctgttta tatcattggt gggatggaag ctcttcagtc
    35041 gagtgttgct tgaatattgc ttgttagatt caaaagaggc tgtgacaaaa aatcgtgtca
    35101 cagcctcttt tataccctgt ctttttttag ttatttattc ttcaacctta tcaatatgac
    35161 ttgatagcct tttctttttc gagtaaactg attttgttag tcatagttat aacaatcata
    35221 gcaataaaag gagaaggtaa gcatatggca tctgttttag tagtcaaagg tcatccccta
    35281 actgcagaag aatcgcgcac agttaaggca ttgacgtctt ttttaacaag ttataaagaa
    35341 aatcatccag atgatgaagt gactgttttg gaactttatc gcgatgatat tccggaaatc
    35401 gatgaagaat tattatcagg ttgggaagca ttacgtgctg gtgcagaatt cacttcctta
    35461 tcagaaagtc aacaacaaaa aattgctcgt ttcaatgaat tgactgatca gttccttgct
    35521 gctgataaaa tcgttatcgc aaacgcctta tggaatctaa atatcccaac aaaattaaaa
    35581 gcttggttcg ataccgtcaa tgtagctgga aaaacgttcc gctatacaga aaacgggcca
    35641 gaaggattag tgacaggtaa aaaagcactg cacatccaat ctaatggcgg tgtatacaac
    35701 ggacaagact ttgcctctca atatgtaaaa ggtattttaa actttgtagg catcgatcaa
    35761 gtagatcagc tattcatcga aggaatcgat tatgaccctg accgtgcaga tgagttgatg
    35821 caaaatgcat tagataaagc tgctgcttta ggtaaatcat tttaaaaatt gagataaatg
    35881 catacaaaag agacagagga aaaaatcttc aaagcagcat ttgtgccttg gatattttcc
    35941 catctgtctc tttttgttat tctttctgct ttttcaacgg attttcttca taaagagaag
    36001 agattgttgt gtaccattca aaaacatgag taatcactac tttagctgct gcataaacag
    36061 gaatccccaa gatcacaccg atgattccga acaatttccc agaagtcagc agaactaata
    36121 aaatcgttac tggatggatt gctaactgat tccctagtac taatggagag atcaagcgtc
    36181 cttctattgt ctgctctact gcaaaaacga tcagtacttt gatgatcatg aatggacctg
    36241 ctacaatggc taaaaagata gccggaatca tcgctaaaaa tgatcctaag taagggatca
    36301 ggtttaaaaa acccgcaata atccccaacg tgacagcata ctctaaacca atgaccgcaa
    36361 aaccaatcat aaacatgatt gccacagcaa aagccacagt tagttgtcct cttatataag
    36421 aagaaacttg actattcatt tcactcagca cattcaacgt cggtttttgc atgcgtgtcg
    36481 gtaaaaattt gacaaaataa ggtgctaatt gtttaccgtc tttcaataga taaaataaga
    36541 taaacggcat cgtcagcaga gccactaaaa tagtcgctac cgcgccgaca aaacttccaa
    36601 gactctgaac tgtactctta gatatatctt ggatcatatc gcctgccgaa ttcatgaact
    36661 tatcacctgc tgtttccaac tgttcgcgaa attggttgaa cagcgggtcc cgcaaaatct
    36721 cggttaattt attatcaatc gtatctacat attgagggaa gttactgatg aagctcactg
    36781 tttgttcctg aatcttaggg atgatcacta cgctccccca tgcgattagt gcaacaacca
    36841 aaacaaacag cccaatgata ctgtaaagcc ttggtacttt tcgcttttca aagtaatcaa
    36901 ctaccggatt catcaagtaa tacaagattc ctgctaaaat gataggcaga cctacgattg
    36961 ctaaaaattg ccagattggt gcaaacaaat atgaaacttt tgtaaataaa aagacgatca
    37021 gcaatattaa caatacaaca agtaaggctg tgactacttg attatttaaa aaccagcgcc
    37081 aaaaccacga taccggtctg ttcttcttat tttttccttc cataattcct cctgttagtt
    37141 ataaaaaatt tggcttccca aaccaatagc aggcagtcta tgcggatgaa gatagattcc
    37201 atttgcaatt tgtccttctt ctggacaagc atcaaaaatc aatgatacgt gagctactgt
    37261 ctttaaattc ccattggcca tcggaccgac ataatcgatc gtatatacct gatcatcaaa
    37321 agcaattgtt ccgcctgctt tcaaagaaaa aggttcgtct tttgatactt cttgaatcac
    37381 cgaatatttt cgtaaggcag ctgttgctgt tttatcgaac aaaatcagca tgggttcttc
    37441 cttgtttatt gcttctgtcc caatttctgt aatctttgcc tgaatcattg cactctctcc
    37501 tttaattgct ttcaaaaaca gtataacaca ttctcattct ttagccgtgt ggaataatta
    37561 tgtgatagaa taaaaacgaa aactatttca ttacaattag gaggaatcag ctcatatgat
    37621 cgaaaagata attttaggaa cctatacgcg tcgcgaaagt aaaggaatct atacgatcga
    37681 ccttgataca gaaaaagaag aattagccaa cctttcatta gctgttgaag aaaatagccc
    37741 tacctatgta gcaaaaagta aagctggcaa tctttacaca gttactagtg tggatggatt
    37801 aggtggtgcc ggtgcgtacg ataaggattt tcattttttg aatgcggtaa cagagtctgg
    37861 cgctcctttg tgttatgtag cggttgatga agcacgtcaa ttactatacg gggcgaacta
    37921 tcataaaggt gaagtaaatg tctaccatat attaggagat ggcgaactga aagcagcaga
    37981 ttcaatattc catcaagaag cgacaggtcc tcataaaaac caagatcatg cgcatgtcca
    38041 ctatacggat ctgactcctg accaacgatt agtcgtatgt gatcttggta cagatcgtgt
    38101 gtatacttat gatgtgtccg aaaacggcaa attgaatctt gtaactacct tcactgcaga
    38161 gccaggaaca ggccctcgcc atcttgtctt tcatccaaat ggacaattcg cttatttatt
    38221 tggtgaatta gacagcacag tccgcgtgct ttcttataat aaagaaacag gtgcttttga
    38281 agaattacag aaaatatcga ccttgccaga agaattcgac ggtgaaaatg gaggggcagc
    38341 tatccgcatt tcaaatgatg gcaagttcct ttatgcttct aaccgaggac ataactcgat
    38401 tgctgtattc gcagtttttg aagatggttc ccacattcaa aacaagcaaa tcatctcaac
    38461 agaaggggat tttccccgcg atttcgcact agatccaagc aataattttg tcgtgtgtgc
    38521 caatcagaac accgataatc tgacattata tcgccgtcat cctgaaacag gattactgga
    38581 attactgaaa aaagatgtct atgtaccaga atgtgtctgt gtttattttg atcgttagta
    38641 agaaaaggag aatgaaatga atcagcaaga cgtattagac agactacggg aagaactaaa
    38701 tattcctttc tttgatggga aaatcgaaga taaagagtat agcgaagagg aatatcagaa
    38761 actcaaagcc gatttacaga actactttga acaatatgta cagaatgtag aaaactaatc
    38821 agctttccat actatctgaa aagaacatca ggacaagact tttccgtcta tcctgatgtt
    38881 cttttctaca aataaaaaac cgccgagatg gttagactcg acggaaaagg agtatttact
    38941 cgattttgag taagtacaag gttgttgttg gttataggta tgtcattatc ataaaggatt
    39001 ttgatcactc tatcataaag gtaatattac atttatatta gataactcta gtttttattg
    39061 ttatcaaaga tttcttttat tttttcaaca acaaattagt cattttttgg atggcagctg
    39121 tcaattggtc tgtcgcttgg tcttgttttg cttcagatgt attgaataga tcttcatcta
    39181 cccctgattc aagatcgata ctttggatca tttcttcaca gcctgctaca tatgaaagat
    39241 aagctttttc aaaattttta tgaatgccca ttgcttttgc tggtggacgc aaagtactga
    39301 ctttcataag cattgcccga tatttttttg ttccttcttc aaataacagg atgatctctt
    39361 caagagtcga tttgtccagt tcacttgttt ttccttcatc aattgcgttt ctgatcactt
    39421 catatttcgg atgcattcct tctccgattg cttctgtttc cttgacgatt tgatcgatgg
    39481 actgcacata atagccaata ttcggtaaca tgttcgtttt cctactttct ccattgttgt
    39541 tattatttat tctacttgtt tttatcctca aatgacaagt acttgaaaaa atttaatggt
    39601 ttctatacag ctcgccattc ttattcgtgc tcaaagaaac catctgctaa actatattgt
    39661 ttccctgcga caatgtcata ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag
    39721 cgattgaatt gcgcttcttt ataccactgt ccgttttgat agtacagttc tcgttcaaac
    39781 gccggaagtt cctgttctaa tgataaaagc agctgataaa agccatttgt tgtctgattc
    39841 gtctgttcca ttagattcgc tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt
    39901 tgcttttcta attctccttt actgtcgaac ataagaaatt gtgtttcatg caatgtttgt
    39961 ttgtcatttt ttgcttggat cgtatctgaa tagatccctg gaagatgatc cccccaaaaa
    40021 acaactaaag tccttctaga aagattcttc aattcttccg tgaactgctt cagtgccgtg
    40081 ctgctatagg aaatatcttg cagataattc tctaagtcaa gtgtcccact tccatctgaa
    40141 gtttttgcag gatagtctag attatcatac tttccatcgt agggcatatg tgtctgcatc
    40201 gtgactaaat gaatgaactg aggtgttttt tcttccttga gaagtgtcat tacttctttg
    40261 taagctgact catcagaaat atatggatta ttctctatcg tatccgtata ggtcatcgta
    40321 cgctcgctga tgaactggtc aaatcctaat gtctgataaa catcctctct tttatacatc
    40381 gaagtattgt atggatggat cgctgtcgtc tggtatgatt gtgcgtttaa cgctgaaacg
    40441 atagaaggaa gctgatccat ttttggtaca agcatcgtgt aaggggtggt gagttgcgca
    40501 ttaaataacg ccatagaaaa accagtcaat gcttcaaatt cgatatttgc tgttccaccg
    40561 ccatagtttt gagaaagcat attgccacta tatgtttgat cagctacttc ataataatct
    40621 gccaatggct caccagatac ttctacgccg tttaatcgac taggatctga gaaactttca
    40681 ctcatcacga acacgatgtt cggcgactct tcctctacag ttttattttt ttcatcggct
    40741 agcttctggt atttttcagt gatttctttg atcttttctt tcgaatatcc ttcaggttca
    40801 tccattggtt ctaccttcag attgtacaaa aacccaccga taaaaccagt gttatagtag
    40861 ttcattttct ggctgtaagg tatccatagt gccgttttat tatatgcttt tcgcaacaaa
    40921 ttatcgggat tgttgaaatt actgatatac cctagcagtc caattgccac aagcagtgtc
    40981 gttaatcgga tcaattggaa tcgccttcct tttttcaggc ttcgaaacat gtaccagcaa
    41041 aacaatccta tcacggttcc tgcggcagct aagatcactg taaacagcat agtgccggtc
    41101 atatctttca gcaatccgat ttcagtgatc atcttcaaat cgtctggata aatcggttct
    41161 tgccgataac tcattttcat ataatttgca aaacctaata caccgatggc tacgacatac
    41221 gtaagggaac ctaaagggac agatcctgct agtgcaatca gaaaaataag aataatcagc
    41281 aagacgagac aggctaagaa gaatttttcc gtatgccaag aaaaagcaaa tttcaacgca
    41341 aggtctacgg atagatcatt ttgacaccac tgcaaaaata aattactaaa aacaacaaga
    41401 aaaaagatac tgatgattgc tgctgttcct tttaaaatct ttttctgttt ggtcgaaagt
    41461 ctgcttatat atcttttcaa atgcttgctc cttcatttct atcaataaaa gaatgttgct
    41521 tctcttattt tagttgtacg aaattcgaaa gtaaagaacg gaaagaagaa ttaatgaaat
    41581 ccaaatattc tctttcttta tggcctttct ggtttcttta ataagcggac ctttcgtact
    41641 ccagaaattt tttctagctg ttgtttcact tgattgagct gttcaaaatc agtactgtct
    41701 atgtcaatca atatgtagac atgctcattc ttatggttac gcgatatatc tgcgatcgct
    41761 agctcgttct cattcagcag agaaaatatc tctgctaata tattggtttt cttttgataa
    41821 aatatagtga atcgaaacgg cgcttggaaa aacattcgtg cggatggata attgaccgct
    41881 tctctaaccg taccgaacaa aagataatct tttaaggctt caactgccaa acgtccgctg
    41941 tctgccaaag cttcctgagt cgatccccct agatgaggca acataagtat acggtcattt
    42001 tctaagaact cttcttctgg aaaatcagtc aagtatttag ccagatgttg tttttccaat
    42061 gcttgcataa cagcatattt atcaacaatt tcacttcttc ctacattgat aagtacagca
    42121 tcttttttca ttttttctat attcgcctga tcgatcaatc cttttgtatc ttctgtcaac
    42181 ggcagcatga ctacgatgta atccgaggta gaaagaactg tttctaaatc tgcttgccga
    42241 acataatcca actgggcatc gcggatagaa taacccaaga catccatccc taagctatag
    42301 caacttagcg ctacttttgt gcctatcgct ccaagaccaa gaagaccgat ggttttgcct
    42361 tgcaattctc gtcccacata agcacttctt ttgttttctg cctgttctaa aatattagga
    42421 cctgttagtg tttgtaccat acgacttgct tcgattatcg gacgggaaga taagagcaaa
    42481 cagcacaaaa caagctcttt tactgcatta gcatttacac ctggtgtatt catgacgatc
    42541 gtcccgtttt cagaagcttt ttcaacgttg atcgtgttca ccccaacacc agctcttgaa
    42601 attgctagca aatctggtgt aagccattca tctttcactt tcgtactacg aataactatt
    42661 ccttcggctg gcaaatcatc tattattttg aaatattccc cttgtaactt ttctatctct
    42721 tgttctggaa tagcatcaat caaatgaacg tgatacataa aaccccctcc ttactaacaa
    42781 gtatctcgaa atttctgcaa ttagacaact gtttaccatt tgacttccta gaagcaaaaa
    42841 gagcagttag agcagagtag gctgagtgat aaaaataagg ctatgacaag ttactgccat
    42901 aagccttatt ttcgagagca aaatattgag ggtgagatat cttgataaat ggtgttccgt
    42961 tagctgacat tgataataag actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc
    43021 tcttcttatt tcttaaaact aagtggcttt ttcacaacct ctttttatct aataaccact
    43081 gtttccaact caactaattt tgcccacgtt tcaatttgat ctgttgttag atttagcgag
    43141 acaacggtat gatgaccgcc acctgcctga atccatgaat ggacaccttc atggaaattc
    43201 ggtttagttt tccataacac acgtgcaaca ggtaaattag gtgcttcagt cgttggttca
    43261 aaggcttcta cttcattgat cagcagttta taatgcgtac cgaaatctgc catcgatacg
    43321 actacccctt ctcctgcttt cccatcaaat acaagacgcg ccggatcttc tctatctccc
    43381 attgataaag gagaaacaac gattcttggt ttcgtcgcag caagcgtcgg atctacttcc
    43441 atcatatggg attccaagat tgcttcttgg ccactagcta gctcgtaagt ataatcttcc
    43501 atgaatcctg tatctttccc tcgagccatg attttcagca accgatcaat cgctgcagtt
    43561 ttccagtcgc cttccccagc aaaaccgtat ccttctgcca tcaaacgttg tacagctagt
    43621 ccaggtagtt gtttcattcc atacaggtct tcaaaattag tcgtaaaagc gttatagcct
    43681 ccttcttcta agaaacggcg aataccgatt tcctgctgag cttgtacttt tacatgttcc
    43741 tcccatgtct tttcttcata atcaccatag tcgaaggtat acaattcctt gtactcttca
    43801 aataattgat cgacttcttc gtctgatacg cgatcgatta cttgtacaag atctccgata
    43861 ccaaaataat caacagtcca accaaattga atctgtgctt ccactttgtc tccttctgtg
    43921 acacccacgt ttctcatgtt gtcaccaaaa cgagccactt ttattccaaa actttcatta
    43981 taggctactg caacatccat ccaatcagca atctcttttt ggatctcagg tcgtttccag
    44041 tagccaacga cgattttatt ttgtttattt aatcgagcat tgatgaaccc atattcgcgg
    44101 tcaccatgag cggattgatt taaattcatg aaatccatat cgatcgtttt ccaaggaatg
    44161 ctttcgttat attgcgtagc taaatgaagc aatggttttt gtagaagctt tgtcccgcgg
    44221 atccacattt ttgctggtga gaaagtatgc atccaagtga tcacacctgc tacttcttct
    44281 cgataattga cctctttcat gatttttgtg atcgtatcag cagtaactgc taattcttga
    44341 aggcggattg gataaggcaa ttggccattt tcattcagtt catcgaccat ttcttgtgca
    44401 tgtttcttta cttctcgcag tgcttcttcc ccatataaat gctgtgatcc tacaacaaac
    44461 caaaattctt tttctccaat attcaacata attagccact ccttttattt gttttgtccg
    44521 taatacgcat tttttccgtg ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc
    44581 tcttctgaaa atgaattgag ctgtctggta aataaattca tcttgcatac ttcatctagt
    44641 acgactgcgt tcattaccgc actttgcgca tctttccccc aagtaaatgg accgtgtccg
    44701 tgtaaaagta cccctggaat tgctaaggga tcaattttgc gttctttgaa tgtttctatg
    44761 ataacttttc ctgtttcata ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga
    44821 gcacaaggta ccgatccata aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc
    44881 aatcctgctt gtgcccagat cgtcgcccat gtcgagtgag tatggacgat tcctccgatt
    44941 tgaggaaatg aacgatataa aacagcatga gtaggcgtat ccgaagaggg attgagttta
    45001 ccttcaatga tatgattgtc caagtccaca acgaccatgt cgtctgctgt catctcttca
    45061 taactgactc cgctaggttt gataacgaaa tatcgtgttt caggatcaaa agcgctcaca
    45121 tttccccatg tatatttgac gagtccgtgt ttaggcaagt caagatttgc ttggtatact
    45181 tcttctttca gttgttccag cattttctat cactcctttt tcttgcaatt gatgaatcgc
    45241 tgttttttca atgtcgatcc cctcacggta acgtctcata aatatctgat agcctgctat
    45301 ttcttctgct gaaggtgtga tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa
    45361 gaagttttct aaagacttcc catgtcgatc catcatatag gctgctaata acgcgattcc
    45421 ccacgctcca ccttctccgg ctgtgtccat cactgtaact ggtgcctcca tagcagatgc
    45481 tagaatccgc tgtccaactt ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc
    45541 aatttggaca ttctcagatt tcaatatatc cattccgatt ctcattgcac caaaagcact
    45601 ggctaaatgg atccgcatga aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa
    45661 taatggcctt ccttcattta ctcctgtaat gttttcaccg gaaaaataac cataagaaag
    45721 taatcccccg caatccgtat cgccttctag cgctttgttg aataataccg tgaataattt
    45781 atctctgctt atctccatac ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc
    45841 attgatatcc gaagagcagt tattcgtatg aaccatgcct actagttctc ctgaaggtgt
    45901 agtgaccatg tcgatttccg gatggacatt ttctagttct ttttctaata cgatcatagc
    45961 gaaggcggaa gtaccagccg aaatgttccc tgtccctttg gctacactgt tcgtagcgac
    46021 cataccggtt cctgcatccc cttctggcgg acaaaccggg attcctggtc tcaaatttcc
    46081 cgtagggtcg atcagataag cccctgattc gcttaacttg ccagcttctt ctcctgctac
    46141 tagaacttta ggcaaaatgt gatccaagtc aatcgctacg tgcgcttttt cgattaactg
    46201 accaaaaata tttttcattt cttgatggta ggttttcgtt gaaggatcga taggaaacat
    46261 gcctgatgca tctcctacgc ctagtacttt ctcaccagta agctgccaat ggacataacc
    46321 tgcaagcgtt gtcaaaaaat taatttcttt cacatgttct tctttattca aaatagattg
    46381 atacaaatga gcgatactcc atctctgcgg aatattgtat tgaaattttt ccgttaatat
    46441 tcgttctgct tcttcagtga ttgtatttcg ccaagtacga aatggtacta gtaactgttc
    46501 cttgctatca aatgctagat aaccgtgcat cattgcactg aacccaatcg acccgattgt
    46561 cgtaagattc tcgccataga tttcgaatac ttctgctgcc atttttcggt aactgacgcg
    46621 cagtcctttc caaatatctt ctaatgaata ggtccagata ccattttcta gtttgttttc
    46681 ccactcataa ctaccagcgg caataggagt aaaagatgaa tcaatcaata cagctttgat
    46741 cctagttgat ccaaactcaa tccctaaaga tgtacgacca tctatgacat ctaacatact
    46801 ttgcgcattt acttccttca taatgattta actccctcct tctcgtcaaa cctgatcaaa
    46861 ccgtcttttt agtagcgctt acaatgatta ttgtatactt ttgtacgtac atgtcaagca
    46921 gttatttaca agaatacaat aaaaaacact tgttttgtac gtacataagt tataaaatga
    46981 aattgcttac aaaatctgat caaccgcctt ctcgaaattc tagaaggaga gaatacttat
    47041 gaaaaaagaa aaaaaagttt cctcatcgtt tatttatttt tttggcgcat ttggcggcat
    47101 tctatttgga tatgatattg gtgttatgac cggcgcctta ccttttttac agcatgattg
    47161 gggtttggca ggtaaagctt ctttgatagg atggatcacg tcttcggtca tgttaggtgc
    47221 gatccttggc ggctctttat ctggtcttct ttctgataag cttggacgac gaaaaatgat
    47281 tcttctttct gcacttattt ttatggctgg ttctgtcctt tctgcatctg cccctcacaa
    47341 tggttcttat tttctgattg cagcaaggat tcttctaggt ttagctgttg gcgctgcatc
    47401 tgccctagtt cctgcttata tgtctgaaat ggctcctgct agactaaggg gaagattgtc
    47461 agggatcaat caagtgatga ttgcttctgg catgctcctt tcctatgttg cagattattt
    47521 gctaaaaggg ttgcctgaaa cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc
    47581 cgcgctgatt ttatttttcg gtgttttagc tctaccagag tcaccgcgtt ttcttatgca
    47641 atctggccgc ttggaagagg caaaaagagt attgaactat attcggacac caaaagaagc
    47701 tgaacaggaa ttcgaacaaa tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc
    47761 ttggcatact ttgtttttag aaaaatatcg ttccttagtc attgccggta ttggtgtagc
    47821 agtattccag caatttcaag gagctaatgc tattttttac tatattccgc tgatcgtaga
    47881 aaaagcgact gggcatgcag cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat
    47941 tttagttgct ggttctttgc tctttttggt tattgcagac aagttcaatc ggcgaacttt
    48001 attgaaaata ggcggtagcg tgatgggact ttctttcatc ctaccagctg ttttaggaac
    48061 tgtactggat gctcatacaa attcgttgtt gattctcttg tttttatgta tttatgtcgc
    48121 attttattct tgcacatggg ctccactgac atgggtaatc gtaggcgaaa tattcccact
    48181 agctgtccgc gggcgagcat ctggcctagc ctcttctttt aattggattg gttctttcct
    48241 agttggcttg ctttttcctg tgatgactgc ttctatgtct caagaaatcg ttttcggcat
    48301 ttttgggatc atctgctttt taggggtttt atttattcaa gagatcgtac cagaaactcg
    48361 tgggaaatct ttagaggaaa tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat
    48421 ttctatccat cattcttaaa gaggttgtga cagaagtggt cagcttcaag aaataagaag
    48481 gaaccgctta gtttccagtc acaaggaaca atttcaaaaa acagcttctc atgtttgatg
    48541 aatattgtga cttgtgacca gttggtagga gaacaccgtt tagtggagag gtggtgaaag
    48601 aaacgttcag ccttgagcga tgacaaaagc tctcacaaac attgcgacga gtaaccgtag
    48661 gagcaataag aggcggtaaa aacccgtctc ttatttttcg ttttctttca ctgtttcccg
    48721 ttcaactagt acaggttcat agagaatatt tggctgtgcc tgtccttttt caatcgttgt
    48781 gacgatccac cgtgcggcat cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa
    48841 ttttttattc cctaaatggc ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc
    48901 tggaatatga tagccatttt ctgtcaactt atctagcatt aaactagcta tttgatcgtt
    48961 atagcaaatg atgccatcta aagcttgttc ttctttcaac cgttgtactg ttttttcagc
    49021 gattgtttct cgtgtttccg tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt
    49081 atactgttca cacgcgctga taaaaccttt catcctgtat tttccttgaa gatcgtcgat
    49141 ttttgtgatg aacaacagac gctgatgtcc tttttttatc aaatattctg ctgccataaa
    49201 accgactttc acatcatcta aacaaatata agaggcattt aactcttcat aagcagcatt
    49261 gatcatcacc atcggaatgc cttgttctct caaagcaaca taaagtgcta agtttgggtt
    49321 atactgatta cttttcgtag gctcaacgat caaaccatct accccataag caatcatttt
    49381 cttcaaacac tctttctcca attcgtgatc gttattcgta ctggctaaaa gcaatgaata
    49441 cccagattct cgtaattcat tttcgattcc tcggataatc gacggaaaaa tataatcaga
    49501 gagatatgtg gtgatgacac cgatcgtttt tgttctgttc aatggtgttt tttctttctt
    49561 atagctgtca tctacatatg tgcccgaccc tttttcttta cgcagatagc cctcatttac
    49621 caacacagcg attgcttgcc gaatcgtatg tctgctgacg tcatatattt cttgcagctt
    49681 gaattctgag ggaatggttg tatttacttc gtattcctca cgaaggatct tttgttttat
    49741 ctcttctgca atatcttgat attttgccat ttgccttgcc cctttttatt tgtacgtaca
    49801 tatatcataa cgagtctgca aggtttattc aagcaaaaat caaggtttct tgtgctcata
    49861 aggcatacgg aacaggttca ccaaattctg gaaatccttg ctcattccac gtaaagcatt
    49921 gcgcgttcgc gtgccgatta ggattgtcta acggatcccc tttttggttc ttctctggtc
    49981 gagcatgata gatcaaaata tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac
    50041 caggaccaaa ttgcttattt ttttcagaag aaacgaatac tggctccgat aatttatgcc
    50101 atgattcttt taataaaaga ttcttgtgct catcagccca cagcaggccc atggcatagt
    50161 tttcatctgt tgcactacca gaatacgtaa tgaatatttt cccattgcga gtaaggactg
    50221 ctggaccttc atttactgaa aaaccccgtt tttcccattc atactcaggt atagaaagga
    50281 ggatctgctt cccctttaac gtccaaggat tatccatttc ggatagataa agattagaat
    50341 tcccagtaat ttcaggatct tgttgggccc acacataata tagctttcct tgagaagaga
    50401 aagttgttcc gtctaaactg aaagtttccc ttttcgtttt tatttgtccc ttttctatcc
    50461 aatctgtatt cattggatct atatgctgat tttcaagtac gaacatgcgg tgatgatgag
    50521 attcatcacg tatagtttta tgattgcttg cagcaaagta aatataccat tttccattga
    50581 tataatggac ttcaggtgcc cagatcaatt cactcatcgg tccttcttca tgagcctgcc
    50641 atacaatcag gcttgcagca tgttctaatt cattcagtga ctttgccctt cttaattcaa
    50701 tcacttggta gcctggaacc gatcctgtaa aataataata accatctgta tgtttatata
    50761 cccaaggatc tgcccgttgc cgaacgatag gattattata aagtgtcttc atcataacaa
    50821 atttcctcac attttcttta ttctaattac attccaagag tatttttcta tccctacatt
    50881 taattcgtca tgtttttcta ttgaaaaata gtttatttca ggttttattt tttctggttc
    50941 ttcacgtgta tttttgtcca acagatcgct actggttaat gtgtagtgct ctccttgatc
    51001 tagcaaattg aaatcatgtt caatcgttat cttttgttgt tcatttgtgc gattgatcaa
    51061 gaaaatagtc agctgattgc cgttatctac gatcacactg tccacataag gaacattttt
    51121 tatcttttca caatcataaa cagggcaaat gatttgttgt ttcaatactt gacctttagc
    51181 atatttcgat aagtgttgca tcacataata agttggattc ctccatgctt gtcctttagc
    51241 atctgtcagt attaaaggta ttgtgtttac taacaatgat tgacaactaa tctttacgcg
    51301 atctgcgtgt cgtaaaatag ccagccctaa tgaaccaaat aataatgtat cttccatagt
    51361 aaagtaacac caatcgatgg gactaccgac ttcaaaatcc ctatgtcttt tttccggttg
    51421 ggcatgtaca ttccattcat caaacgctag atacattgtt ttctcacttc tcaaatatcc
    51481 ttttacataa tcacaagtag caacgatttc ttcaatttga cgatcgaatc gataagcttt
    51541 tgcaaggtat gtcgctgtat catcacgctc atttttagga ggtttagtca agtcttcttc
    51601 atcatactta tcaatatagt tatgcaaagc taaataatct acattctcat aagcttcttc
    51661 taatgcgata cgatcccatt cagggtacga tggtaaacgt ggcgtagagc ttccaactaa
    51721 gattgtttca attgaatcat ccactaactt catgactttt gaggcttcat ttgctaacct
    51781 accatattca taagcactct tagcccccac ttgccatgga ccgtctagct cattccctaa
    51841 acaccataat ttaacgccaa aaggcttttt ttgcccattt tttattctta aatcactcca
    51901 gaaggttcct tttggaaaat tacaatattc tactaaattt tttgcttcat caattccttt
    51961 tgttcctaaa ttcaccgtga atatcggttt cgcttgtaca tgactgatcc atttaaaaaa
    52021 ttcgtggagt ccaaaagaat ttggttctat acctcgccat gctaaatcaa ttttttttgg
    52081 tcgttgttct ttcctaccta cagtatccac ccaatcataa ccagatgtaa agtttcctcc
    52141 aggatagcga atgacaccta attctaattc ctttacataa gagaggacat cttgtctaaa
    52201 cccgtcacca tcagctgttt catggcccgg ttcataaatc cctgtataga cgacacttcc
    52261 catatgctca acaaatgagc caaacagccg agaactgatc tctcctaccg taaaatcctc
    52321 tctgattttt actttcactt cttcgtccaa cagctttccc tacctttcat ttcctatagt
    52381 caagataata gatacaagtt tccatacgcc accagcaaag ataaaaataa aaggagggaa
    52441 aagaaatatg agcagtacaa atcctaataa caatacgcaa caaagaatat ttctcaaaaa
    52501 atcaaaaaat actttccatg cactttgttc aaaaatttgt aaaaatgtga tatctttgtt
    52561 ttcagaaatc acatatatcc aggataaaag aaacatggaa taaataagta aaactacata
    52621 tccactaatt gaaagtacga ttgtctgttc ttctaataat tgattaacaa gaaaataaat
    52681 aatgatcgtt aacagactaa ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa
    52741 gaaaaaataa atgatatttc ctgatccctc acctttccat tgcgccatta cataaaaaaa
    52801 cagcacatgt agcaggcacg atagtaataa caggcaaaca cacgattacc cagagaatac
    52861 ctaataagat gtaatcagaa accacaatca actgtccata aagtttagtc tcaactattt
    52921 tcatcttcct cactctttca ttccagacat agctgttcct tgaacaaaat atttctgtgc
    52981 aaaaatataa accactagta atggcagcat tgaaataacc gttccagcca ttacaggtcc
    53041 ataaaaagaa atgttctgcc ctgtaaataa agctaaccct gctggtaaag ttctcatttt
    53101 tgaactactt gtcaatatca atggataaag caagtcattc caactattca tcagcgtgaa
    53161 aatccctaat gataaaagag taggtttgct caatggcaac atgattttca aaaaaatatt
    53221 aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa
    53281 aaaggaacgc atcatgaaga taccaaatgc agttgtcata cgaggaatga tcaaacctgc
    53341 ataagtatca agcatgccaa gcaaattgac ttcaataaat agaggaatca tgaagacttg
    53401 gaatgggatc atcattgtca gcaaaacgaa atagaacaaa actgattttc ctttgaaacg
    53461 catacgagca aatgcatacc ccgcaagaga atcaaagaat acagaagtga tcactgtccc
    53521 tccagcaaaa atcactgtat ttttgatgta atctaataaa gggatcgttg tccaaacctt
    53581 aatatagtta tctagggtat agctatctgc aaatattcta ggcggaaatg agataatgtc
    53641 tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa
    53701 tataactagc atactaatcg ttcgaatgat ttttttacca ctaactgctt ttcgactatt
    53761 cacagtaact gtaatcgagc tttctggtgc catacaggtt tccctcctat ttcatttgtt
    53821 tttctttccg tgacatgtac acattagtgg agatagctat agttgcaaca atgataaaca
    53881 aaaccgaaga cagtgctgat gcatagccca attcataagg tgctgtaaat cctcgactgt
    53941 aaatatattg aacagctgtt tctgtcttaa attgtgggcc accagcggtt gcaacatata
    54001 cttggtcaaa cacttgcatc gaaccaatca gattcgttaa aatacaaaaa cctaaggagg
    54061 gaacgatctg aggtagcgta atgttaaaaa actgctgtcg atttgttgca ccatccattt
    54121 gagcagattc atacaaagag ggagagattc cctgaatagc agtcaagaga atcgtcatag
    54181 taacaccaaa attcttccaa acagtcatga tggctaccgt cggcatagca agggttggat
    54241 cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa caagtaagga atcgccccta
    54301 cattcggatc aagcaacata gaaaaaataa tcgcaattga cgtcaatgaa caaataactg
    54361 gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt atttttagat aatgctactg
    54421 ctgctaataa accaacgata atttgtgttg gcgtttcgag tacggtgaaa tataaagaat
    54481 gaatcaacga attaactgct cgttcatcac taaatatacg tgtgaaattt tcaaaactta
    54541 caaaatttct tgatgtaaag aaaatattca aatctgtaaa actaatagca aatgttccta
    54601 aaagcggtat gacagtaaat aataataaaa ctaaaaaaga tggagttaaa aacaaatacg
    54661 cagcacgatc aggacgattc caatatttgg tgaatccgtt catatagtcc acctcatctt
    54721 tcattttgat aaaatataca atcttggttt aaacgaatct gaataagata tccgaacaat
    54781 cgctgtgatt tttgcaataa cacctatctg caaatactag gtcgatcatt cagatatctg
    54841 tatcttgcca acagtctatc ctgtataaag ttctgtacta tttttgcagt aattgattga
    54901 tggcatcatc tgcatcgttc atgagtgtct caggatcttc tcctgctaat aatttttcaa
    54961 tcagtggatt gatgatatcg ttattgatcg cagagatttg ggtaaaacct ggtaaatatg
    55021 gctctgcata gtccatttgt ttcgataatt cagatacaat aggatcattt tttacttctt
    55081 catcttttgc tactgaatcc aagtaggcag gaaaaccatt ttcgatactc cattttttac
    55141 caatttcagt tgtattccaa tatttcaaaa actcatagac agcttctttt ttattattat
    55201 ctgtacttgc cggaatggca tatcctacgc catctaaaat agctgttttt ttgtctgaat
    55261 cactcccttg tggtaaggta gtcacattat aatcaatctc gtttgctttc aatccgttat
    55321 ttaaccatgg cccattgatt tcaatggcta gttgaccagc attcattaaa ttgtccattt
    55381 cggcgccaga aatcgattct ggaccagtct tgtctacatg gatcattttt tggatcgctt
    55441 ttaacgtttt aaggttctca ggagacgcaa attcagactt agtataatca tcgttcacta
    55501 atttaccgcc atttgctaac atccagttat ataacggtgc agttccatcc ttattgaaga
    55561 caaaacctga tacattttta ctactatcag ttaattttgg tgccatttct gtcaattctt
    55621 cccaagtagt aggtggttgt tcagtatcta atccagcttg tttaaacaaa tcattattcc
    55681 aatacatata catgccttgt acctgcatgg ggacaaaata ctgtttacct tctacttcac
    55741 ctaattcaat agccgtttct ttaaaatcag atttatctac tccatcatat tcccagaaat
    55801 cgtctaatgg ttgtaccgct ccgttttcta catactgtgc aaaatctgga taatttaatg
    55861 caataaagtc aggtgctttt ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg
    55921 cccaagtcat gatatccatt tcaattttga tgtttttatc atttgtttta ttaaattcat
    55981 taacgatttc ttgcaatatt tcaccatcag atgcagtaaa cccattccaa aaagtcaaag
    56041 tcacttcttt atcagaatca gattctgctg aacttccgcc accgcaagct cctaaaatac
    56101 ctgttgcagc taccaacaaa gagccaatta accatttttt acctttcatt ttttgttcct
    56161 ccttcatctg ataaacgaca tctgaccaca ttccaagata gcggtttcaa taaacaattg
    56221 attttcccat cttcagcttt tacatttgtt gaatgacttg gttttacttg ttcactttta
    56281 ggaagattag ttgcttttgt atcaaatcca ctcatttcag aaaaatcaat cactccctct
    56341 aatgataatt cagacaagtg aacagttaga ttcatttctt cttctccacg gttaactgca
    56401 aatatgacaa gttctttgtc atttattacg gctatacttt ctaaatacgg cacttcttca
    56461 aaatcttccg tcgcatagtt tgctgaacga atttgtgggc ttaacacctc tccttgtcca
    56521 tacacagcag cttgcataaa tggataatag atcgtctgtc tccaagcttc tccgtttgtt
    56581 tctgtcctga ttggtgcaat aacatttacc aattgagcaa gacaagcaat ctttactcta
    56641 tctgcatgtt tcaataaagt gattaataga cagcctaata gtaatgcatc ttcaaaatta
    56701 tagttatctt ctagtaaagg aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc
    56761 gctgaatgat accaaatatt ccattcatca aaggacaggt tgatctgctt cttactatgt
    56821 ttcttcgcct tgactgtatc gcacatcgct gtcacttcag aaatgaaccg atccatgtct
    56881 aaggaacgtg caagataatg agaaagatca tttttttggt tgccataata ttgatgcaga
    56941 gacaaataat ctatttgatc gtacgcttga tcaagaactg ttaactccca atcaccaaat
    57001 gtaggcattt gccgattcga acttccgcat aaaaccacct caattgaatc atctacgagt
    57061 ttcatcgcct tagccgtttc ctctgctaac cgtccatatt cttccgctgt tttatgtcca
    57121 atctgccaag gaccgtccat ttcattccct aaacaccatg tcttaattcc aaaagggctc
    57181 tcttgaccat tcgaacgcct taaatcactc caataagttc ctttaggaaa attacaatat
    57241 tcgactaaat tacgagctgc atctactcca cgagtaccca gattgacagc catattgatt
    57301 tcggccccca catgctgaca ccagttataa aattcatgaa tcccaacttc attcgtttca
    57361 attgtccgcc aagctaaatc caaccttcta ggtctatttg ctatgggccc aatactgtct
    57421 tcccaacgaa atcctgacac aaaatttcca ccaggatatc taatcatggg aatgttcaat
    57481 tcttttacca agtgaatgac gtcttggcga aatccacttt tattagctga tggatgatct
    57541 ggctgataaa tcccttcata tactgctcgt cccatatgtt caataaatga actgtacaac
    57601 cgcggatcaa ctttgcctac acgcttgaca ctactaatca acattgtggc ttccataact
    57661 gacctccttt aatttataca tgaataatac acctcgttga aagcgttgtc aataatataa
    57721 atgttaaact tacttatttt ttatcgattt taattaacca aaatattaat aatgattaaa
    57781 acaaataatt taatataact tttattaaca aatatgttaa tatataagaa aaacctttta
    57841 agaggtgata tatatgcaac tacaaataaa cgaagaatcc ttaccggtat atgaagcatt
    57901 agctagtaaa acaaggatca agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa
    57961 agatttggca aaagaacttg gagtgagcag tgctatcacg acgatgcacg tgaaaaagct
    58021 agaggaagca aacatcatca aaacagaaaa agtaggacaa caaaaaattt cgagccttcg
    58081 tgtcgataaa atcgatatca gttttccaga aaaaatattc aatgcttttg atacgaaaga
    58141 aacatcgata cctattggtc actatacaaa ttatgcaatt gaaccaacat gcggtttagc
    58201 tacgatccat gattttatcg ggaaagtgga tgaaccacgc tattttatgg atcctcgaag
    58261 gatggatgcc cgcattctct ggtttaccag cggatttgtc gagtatcagg cacctaattt
    58321 tttaaataca gaggatacat tggagatgtt ggaagtttct gtggaaattt cttctgaatt
    58381 tcctttttca aatgacaatt ggccttcaga tatcactttc agcctaaatg gtgtggagct
    58441 aggaacatgg acgagccctg gagattttgc tgatatccgc ggtaaatata cgcctgattg
    58501 gtatccagat aatcttaatc aatacggctt gctgaagacg atccgaatta ccaaacactt
    58561 gacgaatatg aatggagaac ccttgtcaaa catcacgatc aacgacatcc ctaaagagca
    58621 agacacttgg catttgcgaa tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac
    58681 tttatttggt aaaggattcg gtaattacga tcaggatatc aaattaaaag tttactacag
    58741 ctgatatttt tcttttctat tttataaaac aaaaacagcc aatccctatg aagggacggc
    58801 tgtttttgct ttattgttat ttccagaaat catccaaaac ggaaattggc aaatgtcgtt
    58861 tatgttgtgt cttgttccac catgcttcga ttttttcttg tgcttgagcg ggaacttctt
    58921 ttccttctag ataatcatca atttcattat atgtgacacc tagtgcgact tcatctgcaa
    58981 tcattggttt cccatcttca agatctgcag tcggaatttt tgtgtataat ttttccggtg
    59041 cattcaatgc ttgaagcaat tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa
    59101 tgtcggctcc accatcacca aatttcgtga agaatccagt aatgttttct gcagcatgat
    59161 ctgtccctaa tacggctcct gctctttctc cagctacggc atattgcgta atcatacgtt
    59221 gacgtgcttt gatatttcct ttgttaaaat cgctgattcc cacacctgct tcgcttaata
    59281 cttgtacttg tgcatcaaca gctggtttga tatttactcg cagactaaca tcgggctgga
    59341 taaagtcaag agcagctttt gcatcttctt catctgcttg ttcaccataa ggaagacgaa
    59401 cagcgataaa ttgatagttt tcatcttttg tttcttcacg catttcttcc atcgttagtt
    59461 gcgccaaacg gcctgctaaa gtagaatctt ggccgccgct gatacctagt acaaaagttt
    59521 taagaaacgg atattttttc aaatatgctt tcataaagtc aatactttta cgtatttctt
    59581 cttttggatc aattgttggt ttgacaccta attcattgat aatttctgtc tgcaggctca
    59641 tttattctcc tcctatgctt ctgtatgaag ttctgctacg ctttttttca ttcgttccat
    59701 gatagccatt ttatggttcc agcattcagt agacagatcg actgggtatt tttgcggatt
    59761 caagtctcgt ttatactctt cccatagtga atctagattt tcttttgtat attctttgat
    59821 ttcgtttagt gcaggtaatt cgtaaactct ttttccttcg ataaagatat cttgtaaaac
    59881 tgggcgagct gtaaagtctc gaacagtttt attgataaac gtatgcactg gatggaacat
    59941 gaagatagct tcttcttctc gagggtcttc atcccataat gtcacgtagt ctccttcaga
    60001 tttcccatca aaatttcgag tgatgcgcca cacttgtttt ttccctggag tagttacttt
    60061 ttctgcattg cttgataact tgatcgtatc catcattttt ccttcatcat cttcgatcga
    60121 tactagcttg aatactgcac caagggctgg ctgatcatag gctgtgatca attttgtccc
    60181 aacaccccat acatcaattt tagctttttg catctttaaa ttcaaaatcg ttgcttcatc
    60241 caagtcattc gaagcataga tttttgcttc cgtaaatccg gctgcatcta attgttcccg
    60301 aacacgctta gatatatagg ccatatctcc actgtcgatg cgtacgcctt ggaagttgat
    60361 tttgtcacct aattctttcg ccacgcgaat cgcacttggc acacctgatt tcaacgtatc
    60421 ataagtgtcg actaagaaca cgcagtcttt atgggtttta gcgtaggcca taaatgcttc
    60481 ataatcattt ccataagatt ggactaacga atgagcatgg gtcccgctag ctggtatccc
    60541 aaatatcttc ccagcacgta cattgcttgt tgcatctgct ccgccgatat atgccgctct
    60601 agttccccag actgctgcat ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg
    60661 atcttctccg atgacagatt tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt
    60721 gaccatattc aacagtgctg tttctactaa ttggcagtgc gctaaaggcc cttccacttg
    60781 gattaatggt tcatttgcaa aaactagttc tccttcacga gcagaacgaa ctgttgcttt
    60841 aaattcaaaa ttttgtaaat actccaaaaa accttcagga tagacttcta atccacgcaa
    60901 gtaagcaata tcggaatcat tgaacgtcag attctctaaa taattgacta accgttccaa
    60961 tcctgcaaaa accgcgtatc cgtggttaaa aggcatttct cgaaagtaac attcaaaaac
    61021 agcatgtaaa tcagctcgac ctaattccca gtatgtctgc atcatattga tttgatatag
    61081 atccgtatgc agcgtcaaac tgtcgtctgg gtaagtatta ttcatcttgt attactcctc
    61141 ttggcaagtt ttcatttcct acccattata acaaaaaaaa ttaataggaa aagaaatctt
    61201 ccattttttc tgaaaaagca catgttttac ggatatttct tgctaaaaat gaactccatc
    61261 tgctaaaact atcctttctt attggtattt aacctaaacg agaaagaata cttattttat
    61321 tcaatgtatc cgagacacga agaaattagc gttttctatt tttcttactt gataaaataa
    61381 gtagattttc ctactacaat cgtagccgtt aagataccga tgattgaaca aatataacca
    61441 aatgtctcta caacataatc aatggcaaaa gtttttgaag caataatacg tctttccttt
    61501 tttgcttctt atccccaaag aaacggatat aacttattat gtattgaatt tttttggaaa
    61561 ggcgaattat gacaagatat accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt
    61621 ttttaaaaag atacaaagta agaaagcagc tgtcaataga atggaaacaa aaatggcgag
    61681 acatcaatca gtctcgcctt gttttttatt gtatacatta gatatggagc aggttaccat
    61741 aaagatgatt attagtcgtt attaaataga atccttcgta tttttttgtt ctatcactac
    61801 aatatcttta tttggacgtt tgaattgtat tccggcacgt tctagctcct gtttcactcc
    61861 tacatggact ttgacatctt ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat
    61921 ttcttgcctt gtgatttttt tcttcgtatt gatcatcacg acttgctttt tatcatatgt
    61981 gtctaccaat agcagttgtg ttcgatctaa cgaaaataat ttgtaataat taatagccgt
    62041 attcccaaaa aagaattcca ctactttagg ataaaatgtc ttaatattta aattactatt
    62101 cacaggtgtt tcgattagct tcatctcatt cctcttttct atatagtcga tttagatcca
    62161 atcttaagtt atgttaccat ctttcctagc attaaggaat cggttacaat tgaaccttta
    62221 aagacaaaag gatttacctt ttatcactta atagtataac ataatttcgt gagaaaaaaa
    62281 tacaattttt tcttgaccat ctcgttacgt aaaggattat gcttacattt gtcaggagga
    62341 aaaataatgg agtacacaat aaagaaaatg tcagagatat caggagtcag tccgagaacc
    62401 ttgcgctttt atgatgagat tggactgcta aaaccagcta ggatcaattc ttcaggttat
    62461 cggatttatg gcaaaaaaga agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg
    62521 gcattcaagt tggatgatat ccaagaggtc ttagacaatc catcttttga ccaccagaaa
    62581 gctttaatca agcatcagca aatgttgctg gaaaaacggg cacaaatcga taccctcttg
    62641 acaactgtcc aacagacact tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa
    62701 aaatttgaag ggtttaaaca acaaaagttg aaagaaaatg aagaatccta tggacaagag
    62761 attcgacaaa aatacggaaa aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg
    62821 gataaagaaa cgtatgaaga aatgcaaact gttgaaaagc agatgttatt tgatctcact
    62881 ctttacttaa aagaaccaga agatgaaagc ttagcagacc gtatcttcca agctcataag
    62941 aagtggttct cttacagctg gcctgaatat cagccaaaag cgcataaagg actaggaatg
    63001 atgtacgaag ccgatgaacg gttcactgca tattatgacg agcgaagtgg tcatggtgca
    63061 gcaaaagctt taaatgaaat tatccaaaaa aaggcgtaat cttttacatg atccaacaaa
    63121 aaagtacacg taaaatgacc taataacaaa acagcagaca cccaagttag atttttatgc
    63181 taacttttgg tgtctgctcc attctttcac aagttttttc atcctacttt aaatatattc
    63241 atcgttgaat aaacgtataa ggtattttca ctttatcctt tttttctgta agaaacaatt
    63301 gaaatgccat ttctccgcaa gcttccagat ggtggtcgat ggttgggaaa ccaagaagct
    63361 cgctgatcag caaattatcg cgtccaatca ccactggtgt cttatcttgc agatagcttt
    63421 gcaagatgcc tgctgcaacc atatccgaat ttgtaaagac accatcaact ccaagctctt
    63481 taaaaaagaa gcctgcttgt tttcctttct ctgcatctat acaatcccaa aaaacaagtg
    63541 attcatcaaa agatggaaaa agttgatgac atagctgcaa agtgattttt gagttataac
    63601 ttagcctgcc acttcgcccc aacgtgatgc ccaattgctt tataccttgc tgcttcaaat
    63661 aagtaagacc ttctgtaatt gattgttctc gatcaataaa gacacagctt gcctgttttc
    63721 ccttgatttc ttcacaaaaa actagaggtc catatttttg atatgccaat aaatcatcta
    63781 gtgtattggc acgagacgta atgatcaatc catcgaaagc tttagctgca aattcttcaa
    63841 gataccttcg ctctttttct ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat
    63901 aatcaaacgc tgcttcaatg atcccactga tcaactgatc aaaatacgga tgattggtat
    63961 aaggcagaat cactccaatg cttttcgttt ttccataact taactgtctg gcatttgcat
    64021 tagggatata atcgacttct ttcatgaccg caagtatttc tttccttttt tcctcatcta
    64081 catatggatg tccattgatt acacgtgaaa tagtagaaac tgaataacct gtgagctttg
    64141 cgatatctcg aatgtttgcc actgctgctt cgccacctat acaaaaagat tttcttgtaa
    64201 atagtctacc acagctggac cgcattcaaa aaactgatac atttttcctt catgcgaaaa
    64261 actgatccgc tgcccgcccc aatatatatc ttttgtcaaa ttttcgattt cattcattgg
    64321 tatttctttt actatattcc tattcaaccc ttgaaaactg tacaacgtta atacggatcc
    64381 cttgaactcg acaaacgcat aaaaataatt gttcgttgtg ccatctgtta tctctacata
    64441 agtattcatt gcaagccctc cttttgatac tttgagtata gtgtatgaaa cgcgtctcat
    64501 agcaaggagt ttgttttgcc aaagaaaagt tttattcctc tttcattatt accaaagtct
    64561 cttattttaa attgaataca ttataatgat actgtaaata tatctttatt attagtgaat
//
LOCUS       DACDHL010000007        59815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDHL010000007 DACDHL010000000
VERSION     DACDHL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.007"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..107)
                     /locus_tag="IV228_000464"
     rRNA            complement(<1..107)
                     /locus_tag="IV228_000464"
                     /product="16S ribosomal RNA"
     gene            26..169
                     /locus_tag="IV228_000465"
     CDS             26..169
                     /locus_tag="IV228_000465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716406.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(629..1939)
                     /locus_tag="IV228_000466"
     CDS             complement(629..1939)
                     /locus_tag="IV228_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8716407.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1951..3369)
                     /locus_tag="IV228_000467"
     CDS             complement(1951..3369)
                     /locus_tag="IV228_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8716408.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3609..5177)
                     /locus_tag="IV228_000468"
     CDS             complement(3609..5177)
                     /locus_tag="IV228_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8716409.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5181..5591)
                     /locus_tag="IV228_000469"
     CDS             complement(5181..5591)
                     /locus_tag="IV228_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8716410.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5588..6511)
                     /locus_tag="IV228_000470"
     CDS             complement(5588..6511)
                     /locus_tag="IV228_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8716411.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6508..7386)
                     /locus_tag="IV228_000471"
     CDS             complement(6508..7386)
                     /locus_tag="IV228_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8716412.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7373..8710)
                     /locus_tag="IV228_000472"
     CDS             complement(7373..8710)
                     /locus_tag="IV228_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8716413.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8713..9816)
                     /locus_tag="IV228_000473"
     CDS             complement(8713..9816)
                     /locus_tag="IV228_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8716414.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9890..11398)
                     /locus_tag="IV228_000474"
     CDS             complement(9890..11398)
                     /locus_tag="IV228_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8716415.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11364..12056)
                     /locus_tag="IV228_000475"
     CDS             complement(11364..12056)
                     /locus_tag="IV228_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8716416.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12078..13052)
                     /locus_tag="IV228_000476"
     CDS             complement(12078..13052)
                     /locus_tag="IV228_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8716417.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13352..14224
                     /locus_tag="IV228_000477"
     CDS             13352..14224
                     /locus_tag="IV228_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8716418.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14682..16880)
                     /locus_tag="IV228_000478"
     CDS             complement(14682..16880)
                     /locus_tag="IV228_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8716419.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            17112..18104
                     /locus_tag="IV228_000479"
     CDS             17112..18104
                     /locus_tag="IV228_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8716420.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18164..19585
                     /locus_tag="IV228_000480"
     CDS             18164..19585
                     /locus_tag="IV228_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8716421.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19578..21077
                     /locus_tag="IV228_000481"
     CDS             19578..21077
                     /locus_tag="IV228_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8716422.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            21116..22510
                     /gene="uxaC"
                     /locus_tag="IV228_000482"
     CDS             21116..22510
                     /gene="uxaC"
                     /locus_tag="IV228_000482"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8716423.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22669..23352
                     /locus_tag="IV228_000483"
     CDS             22669..23352
                     /locus_tag="IV228_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8716424.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23354..24190
                     /locus_tag="IV228_000484"
     CDS             23354..24190
                     /locus_tag="IV228_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8716425.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24201..25955
                     /locus_tag="IV228_000485"
     CDS             24201..25955
                     /locus_tag="IV228_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716426.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26231..26572)
                     /locus_tag="IV228_000486"
     CDS             complement(26231..26572)
                     /locus_tag="IV228_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8716427.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26640..28811)
                     /locus_tag="IV228_000487"
     CDS             complement(26640..28811)
                     /locus_tag="IV228_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8716428.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            29022..29879
                     /locus_tag="IV228_000488"
     CDS             29022..29879
                     /locus_tag="IV228_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8716429.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29950..30807)
                     /locus_tag="IV228_000489"
     CDS             complement(29950..30807)
                     /locus_tag="IV228_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8716430.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30792..33494)
                     /locus_tag="IV228_000490"
     CDS             complement(30792..33494)
                     /locus_tag="IV228_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8716431.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33507..34796)
                     /locus_tag="IV228_000491"
     CDS             complement(33507..34796)
                     /locus_tag="IV228_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8716432.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34946..35992
                     /locus_tag="IV228_000492"
     CDS             34946..35992
                     /locus_tag="IV228_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8716433.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35994..38147
                     /locus_tag="IV228_000493"
     CDS             35994..38147
                     /locus_tag="IV228_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8716434.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38620..40071)
                     /locus_tag="IV228_000494"
     CDS             complement(38620..40071)
                     /locus_tag="IV228_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8716435.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAL
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(40068..41792)
                     /locus_tag="IV228_000495"
     CDS             complement(40068..41792)
                     /locus_tag="IV228_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8716436.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41785..42399)
                     /locus_tag="IV228_000496"
     CDS             complement(41785..42399)
                     /locus_tag="IV228_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8716437.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42744..44201)
                     /locus_tag="IV228_000497"
     CDS             complement(42744..44201)
                     /locus_tag="IV228_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8716438.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44242..45162)
                     /locus_tag="IV228_000498"
     CDS             complement(44242..45162)
                     /locus_tag="IV228_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8716439.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(45174..46121)
                     /locus_tag="IV228_000499"
     CDS             complement(45174..46121)
                     /locus_tag="IV228_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8716440.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46600..47319)
                     /locus_tag="IV228_000500"
     CDS             complement(46600..47319)
                     /locus_tag="IV228_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8716441.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47368..49014)
                     /locus_tag="IV228_000501"
     CDS             complement(47368..49014)
                     /locus_tag="IV228_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8716442.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49193..49783
                     /locus_tag="IV228_000502"
     CDS             49193..49783
                     /locus_tag="IV228_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8716443.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49875..51380
                     /locus_tag="IV228_000503"
     CDS             49875..51380
                     /locus_tag="IV228_000503"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8716444.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51464..52816)
                     /gene="celB"
                     /locus_tag="IV228_000504"
     CDS             complement(51464..52816)
                     /gene="celB"
                     /locus_tag="IV228_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8716445.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52816..53334)
                     /locus_tag="IV228_000505"
     CDS             complement(52816..53334)
                     /locus_tag="IV228_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716446.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53350..53676)
                     /locus_tag="IV228_000506"
     CDS             complement(53350..53676)
                     /locus_tag="IV228_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8716447.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53689..55668)
                     /locus_tag="IV228_000507"
     CDS             complement(53689..55668)
                     /locus_tag="IV228_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8716448.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55689..56003)
                     /locus_tag="IV228_000508"
     CDS             complement(55689..56003)
                     /locus_tag="IV228_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8716449.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            56171..56464
                     /locus_tag="IV228_000509"
     CDS             56171..56464
                     /locus_tag="IV228_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8716450.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56542..57654)
                     /locus_tag="IV228_000510"
     CDS             complement(56542..57654)
                     /locus_tag="IV228_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8716451.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            58041..58220
                     /locus_tag="IV228_000511"
     CDS             58041..58220
                     /locus_tag="IV228_000511"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716452.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58414..58584
                     /locus_tag="IV228_000512"
     CDS             58414..58584
                     /locus_tag="IV228_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8716453.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(59008..59748)
                     /locus_tag="IV228_000513"
     CDS             complement(59008..59748)
                     /locus_tag="IV228_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8716454.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17873 a        12225 c        10465 g        19252 t
ORIGIN      
        1 tctttttccg gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg
       61 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct
      121 tgcgattgtt aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttatt
      181 gtcttgaatt gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca
      241 aaggtctact tcatcgagag tgtttcccga ttcatttttg gctgccgtag caacttttat
      301 atcatatcag agagacaaac gattgtcaac tcttttttaa aagttttttt cgatttcgtt
      361 tttcgaaatc tttcagcaac tcatttatca taacttatcg tttgttattt gtcaagaact
      421 tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg gttcttttta acgacttagt
      481 tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat aaaatgtttc
      541 aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc aagctaaaat
      601 attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg tttgcagctg
      661 tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt actgcttcga
      721 ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct tctataggct
      781 tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct ggagaaaggc
      841 atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt gattcaggta
      901 tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg ctattttttc
      961 tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca tcaatcatat
     1021 tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa gatttcattc
     1081 gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca ctaactccag
     1141 cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat ctagcaacct
     1201 cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca gacttaatag
     1261 caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt gttgaagaat
     1321 caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact ttttctacta
     1381 atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga aaaatcaaat
     1441 cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt ttaatagcat
     1501 aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg cttttttgat
     1561 cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt ccttttcctt
     1621 ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat ttcatttcta
     1681 cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc cccttgagga
     1741 ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct ccgactaaat
     1801 aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg attgcttttt
     1861 gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa gcattgataa
     1921 tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt tcaattcttc
     1981 accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa tgattgcgga
     2041 tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt cttgtactaa
     2101 aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg ccagcataaa
     2161 tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat ttactccata
     2221 tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca cgtcagtaat
     2281 gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa ataaaagaat
     2341 cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca tacatgaaga
     2401 agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca gctttgtagc
     2461 aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag acattgcttg
     2521 tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta aaatctgatt
     2581 gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag ctaacaaacc
     2641 ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc tttcagtagt
     2701 caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga ttgaaatcca
     2761 aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat acattaaaat
     2821 cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc ggaacgtgga
     2881 caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc catacgggat
     2941 attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat aagcaatctt
     3001 caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca ctaagcaaaa
     3061 tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta actctgagac
     3121 atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg aaatcaagaa
     3181 taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa tcatcaagaa
     3241 actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt agcctatctt
     3301 atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa cagcattttg
     3361 tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt ataactcttt
     3421 ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga cagataaaaa
     3481 acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta cataacaata
     3541 tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc atttattaaa
     3601 gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc agcataactt
     3661 cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt aaaccctcct
     3721 gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt tgatacgtct
     3781 ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt cttcttttct
     3841 gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc tatcatacgt
     3901 aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt ggattcattt
     3961 aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc aaatgctgtc
     4021 tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc gaaggtagat
     4081 tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt atcacaccaa
     4141 tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc ttcaaatgta
     4201 tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat cattatttct
     4261 tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt ccaatacact
     4321 tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct ggtacaaaga
     4381 tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc tgtacattgc
     4441 aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc aagctcgtaa
     4501 ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc tgtaccgcct
     4561 tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc ctccgttttc
     4621 gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt cttctctgca
     4681 tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg ataatcccat
     4741 agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga aggatcaaag
     4801 ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga tgcagtgatc
     4861 aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt gtaaacaaga
     4921 taaaacaatc catcagcata actgatttgt ggcgcccata tacttccatt tgtaggattt
     4981 ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt gactaaattt
     5041 ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat aatgtaatac
     5101 cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat aggatttttc
     5161 actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata taaactgaat
     5221 gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt gccaatcaac
     5281 aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt tcctttagca
     5341 gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac ggcaaacaga
     5401 ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga atgatctaca
     5461 gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa agtaactgct
     5521 aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa tagttcccat
     5581 tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt cttttgatcg
     5641 atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt ttcttgtcat
     5701 cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct tcaaataaga
     5761 catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca ggttctgcag
     5821 tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta gatgctgctg
     5881 tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga aaaacggcat
     5941 ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca taggtctgac
     6001 gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct ttttgcaaat
     6061 caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag ttttttaaag
     6121 atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct cctggacctg
     6181 ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag aaaaaagtag
     6241 cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca aaacgattac
     6301 cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg ctttctatat
     6361 attcatcata tcttccttct tttactatca agacctttgg atggctatcc ggcaggcctg
     6421 ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct atagcattct
     6481 ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt gtttccaaat
     6541 atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag aaaagttttc
     6601 tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca gtacgccttc
     6661 gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg gagcgatttt
     6721 ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt aataaaaccc
     6781 atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg ccgctagatc
     6841 aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata agcctcccat
     6901 agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct ccaaataatc
     6961 gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg ttaataaaaa
     7021 ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc aagcatcaat
     7081 cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag aagctaagta
     7141 aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt gagaatgcag
     7201 gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg ctggtactgt
     7261 ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt gtttttctcg
     7321 gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa aatcattcaa
     7381 ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat tgatttgatt
     7441 ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt ccctcgtgat
     7501 tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa aatccttgtc
     7561 tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca tcttgggcca
     7621 tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct aatccataaa
     7681 taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg atccttctga
     7741 atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac ggttctttcc
     7801 ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca ttatccatga
     7861 ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta gatttcaatc
     7921 gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta cgaatactac
     7981 cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg caatttgtga
     8041 tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc ttgattgcaa
     8101 tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg catgattctg
     8161 gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag ttatcaaacg
     8221 ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc aatttaatat
     8281 ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga ggataagcga
     8341 gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca tctaatgtac
     8401 cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa gcataaactt
     8461 ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga tcgtcagaga
     8521 atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa aataaagctc
     8581 ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt gctgcagcat
     8641 caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca aatgtcaaaa
     8701 tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg caccaacgcc
     8761 tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac catctcgctg
     8821 atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc agccttcttt
     8881 tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt gtggaagcca
     8941 ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa caaacatact
     9001 actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta cttgatacca
     9061 tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt ctagaatttc
     9121 tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg ctgcagccat
     9181 caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt ttggatcagc
     9241 ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt ttggatcgaa
     9301 tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt tttcttcact
     9361 aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat ccaaccaaat
     9421 ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg gatggtttgc
     9481 caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg tttctttgta
     9541 caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt caacgctgta
     9601 gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta tgaaatcaaa
     9661 ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta cgacgccata
     9721 gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg ttctttccat
     9781 gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt taaaaattaa
     9841 aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat tactgtgccg
     9901 ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt gctttccagt
     9961 cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat tgcaccgtag
    10021 catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt gaatccaatg
    10081 gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga ttagagaatt
    10141 ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga gagcttgata
    10201 acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa ccatcatcat
    10261 tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca tcttcttcta
    10321 aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt acttgagatt
    10381 tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga taaccgccgc
    10441 cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga ataccttgac
    10501 ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt cttccttgtg
    10561 caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc aaatagccat
    10621 tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta tcaaattctg
    10681 gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa taagaagtga
    10741 aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa ccaactgtgt
    10801 cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact tcataaagct
    10861 catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt cgaatgacta
    10921 agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg tcgatactcg
    10981 cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt aaatattctg
    11041 atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt ttcaacatcg
    11101 caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt gttaatcttt
    11161 cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt ttttcttcga
    11221 tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt aaccaagtga
    11281 ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg ccgcatgcgg
    11341 caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct tttttcataa
    11401 attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc tgtatatgag
    11461 ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc ggaaaagaag
    11521 agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat cagataaatc
    11581 cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc gaataaaaat
    11641 gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat tgggaaaata
    11701 tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct aagataccaa
    11761 ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata tatccagctc
    11821 atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt cgtcaaagga
    11881 aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata cgatgcagga
    11941 ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa attcaaataa
    12001 accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg cagcatatct
    12061 ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc aatcttatta
    12121 gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc tgtactgaaa
    12181 cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat ttctgcaacg
    12241 cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc taatgtctga
    12301 ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa tgtaacatgc
    12361 catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa ctgtgtatcg
    12421 actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt ccagatcact
    12481 tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat tttttcaaat
    12541 cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa tacataagta
    12601 atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt ttgaatcccg
    12661 tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat tggcatcgga
    12721 aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa tgtaaagaat
    12781 gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact tcccattatt
    12841 cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg aagataccta
    12901 aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt attcgttttg
    12961 attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga tagcgtcagt
    13021 tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt gtgaatgttt
    13081 gctgcttatg acttcatgat agataactat ctgaattacg tatataatca tttttaccgt
    13141 aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa agtgtatttt
    13201 ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca cttagcatag
    13261 gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga aacagaaaca
    13321 tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta taggagaaag
    13381 aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct gtatcgttcc
    13441 ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg cgaaaaagtt
    13501 tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt ctacgccgac
    13561 catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga tcgtcagtat
    13621 gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg gtcgaggttt
    13681 cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc caacgttctt
    13741 agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc ctgtggctgt
    13801 taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg atagtttgga
    13861 agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa ttatgctgcc
    13921 attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt actggaacac
    13981 ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac agattctttt
    14041 acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg ttgatgtgat
    14101 tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc ctatgctgat
    14161 tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt ctgtcaaagg
    14221 gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct tcaagaaata
    14281 agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt atttttcgaa
    14341 gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca gccttgagcg
    14401 atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata agaagcgatt
    14461 ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag cctgtctctt
    14521 gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt tctttttgcg
    14581 tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt ctaacttctt
    14641 ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata tttttttcga
    14701 tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt ttgcggattt
    14761 cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt caaccatttt
    14821 tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc tgtttccttt
    14881 ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat cacatctaaa
    14941 gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga caaattttta
    15001 aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa ttgttctaaa
    15061 acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata aatttgtttc
    15121 atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac gagttcattg
    15181 atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc aaacaatgca
    15241 tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat ggcttcaaac
    15301 aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc agcaggaacg
    15361 atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata cagggcttct
    15421 tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt actgatgcct
    15481 atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg gcaatactcc
    15541 atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt ggcttgcatt
    15601 tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc cgtgatcatg
    15661 ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc taacagatcg
    15721 atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa ttgctgcaac
    15781 cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac aaatagcgtt
    15841 tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc tcctatgatc
    15901 cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt tgatttaaga
    15961 ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa taagtaggaa
    16021 agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat tctcagattg
    16081 cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca attattataa
    16141 tctgatttca gtactatata cgttttatct ttttctctta tcactttcag ctgattgtaa
    16201 tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt ctgagtagta
    16261 aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc accaacaact
    16321 tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc tttttcaaac
    16381 attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa taaattattt
    16441 ttgttattta tgtacttctg ttgatacttt tcaacttcct cttcggtcag ttgactcaac
    16501 gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt attatcatcc
    16561 ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt ggagtaatct
    16621 gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc atacgtagaa
    16681 aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg cctgagcaaa
    16741 ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg gtaaatgaat
    16801 aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa tattttataa
    16861 aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag gctttcattt
    16921 ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa gaatagcata
    16981 attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat caaagatcta
    17041 ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga aaaagtaagg
    17101 aaaatgatgg aatggtaacg atcaaagata tcgctaaagc agctggcgtt tctcatacga
    17161 cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa caaaaaatac
    17221 gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt ttagtgaatc
    17281 aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact tcatcaaatt
    17341 tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac agtttatctg
    17401 ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac gatggactga
    17461 tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa gaaaaagaat
    17521 ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac gtcactgccg
    17581 atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga catacgaaga
    17641 tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa aaaggggtat
    17701 tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta acaggagatt
    17761 acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct gacctgccta
    17821 ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct gtacatttag
    17881 ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct ccagtcgtct
    17941 cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg tgtcaagaag
    18001 gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct aaaattgagc
    18061 tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca agtaatttta
    18121 tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa aactgacaaa
    18181 agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg aaggcaattt
    18241 tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat tgtttaaagg
    18301 taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga accagcaaga
    18361 taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg atacttccga
    18421 gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg cttatctatc
    18481 tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg caggaatcca
    18541 attcgtggag actgataatt taacagatac tcccccacag agcttcccgg gcaaactgac
    18601 agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca ttccttgcga
    18661 actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg cagaaaaatg
    18721 ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct gttgtagttt
    18781 agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca atcaacggca
    18841 taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg tgatcgaagg
    18901 gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg tcatcatcac
    18961 agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac cgcatacagc
    19021 gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa tgaaagatcc
    19081 tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta tgctttcttt
    19141 acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct tgaatccttt
    19201 tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca gcacccgtct
    19261 gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt taatcactgt
    19321 ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac atgatgatga
    19381 aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta ttcctcgatt
    19441 attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg tgacagatac
    19501 agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat tacaagaaat
    19561 gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta aatcgacgag
    19621 acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg attgatcagc
    19681 aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg ggtgatttga
    19741 aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg acagacgtaa
    19801 ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt gaattggatt
    19861 atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga acatttcaag
    19921 gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta gtacctaccg
    19981 ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa aaacatccag
    20041 atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt tcgcagcttg
    20101 gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat cccaatgccg
    20161 gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa ttcaaaaaaa
    20221 ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat gtttacgatg
    20281 agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa aatgaccagc
    20341 gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt tctgatggtc
    20401 tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg attgctcaag
    20461 gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa gtattgatgg
    20521 cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat gacttcaaac
    20581 agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt aataaagcag
    20641 gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca ggaagctctc
    20701 ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt agtttattgc
    20761 aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat tgccagttgg
    20821 tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg gtcaaggtat
    20881 ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat gcaggcgtct
    20941 tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc atcgctgtag
    21001 ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc atttttaaaa
    21061 acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa gatttatgtt
    21121 tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc attcctacgc
    21181 aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa tttacgaaaa
    21241 caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca aatggcgctt
    21301 gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg attttgaaaa
    21361 atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt acagttggtc
    21421 acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag aaaatgcaga
    21481 gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc gtcgggaaat
    21541 cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag acgacttgca
    21601 ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt cattccgtcc
    21661 agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac tactggaaga
    21721 agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag ggcagcggat
    21781 agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca tcttacgcta
    21841 taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag ctaacaaact
    21901 attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt tgacccactt
    21961 ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta actgtaatac
    22021 acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg atctttcttt
    22081 gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt tgccaaagac
    22141 gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga tgggctgttt
    22201 ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca acgatactcg
    22261 agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg ggaattttgt
    22321 cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat atttccgtcg
    22381 tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg aagatgaaca
    22441 acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt actttggctt
    22501 ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt gaaataacgg
    22561 aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt cttcttctct
    22621 tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat gcctactatc
    22681 catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc cgagacaggt
    22741 tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa ccaagcaaag
    22801 aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct agcttcacag
    22861 tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa aatccaagaa
    22921 gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt ttttgaatgc
    22981 gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg agattactta
    23041 gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat gcgtgcattt
    23101 gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga attgttccag
    23161 accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga tacttgtaaa
    23221 aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc tgataaagtt
    23281 gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa ttatttacaa
    23341 aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt tagatgttcg
    23401 aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata tccaatttgc
    23461 accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg ggagtgctgc
    23521 ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag gatgctatat
    23581 caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt tcaaacagca
    23641 aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg gtagtgtctc
    23701 tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca gaaattctat
    23761 cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg aagcagggat
    23821 caatcatcca atctataact atcagttagc taaacgttta atcgatgaag tagcttcacc
    23881 aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc atgctgatca
    23941 ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag cgcttcatct
    24001 caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca agggctgttt
    24061 agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt acgccactct
    24121 tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt ttatttacca
    24181 agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa aaatatcatg
    24241 caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt tttgccatgt
    24301 caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc gctattgcca
    24361 atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt tatttcaacg
    24421 cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt catgtaaaat
    24481 accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt atcaagaaaa
    24541 tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc cagtcaaaga
    24601 tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc agcggattaa
    24661 atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt cctgttgttt
    24721 tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt ttccgtaaaa
    24781 ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct gcaaaagtga
    24841 tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa cacgaaacaa
    24901 ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta gatatcctgc
    24961 aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg atttgtttga
    25021 ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc gtgatgtatc
    25081 agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg attcctacga
    25141 tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct ggcgatgtag
    25201 aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt tttgaggatg
    25261 tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct ctagaggtga
    25321 aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct acactcctta
    25381 acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat gatcagcctt
    25441 tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct caaaccactt
    25501 tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac gtatcaaaag
    25561 aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac cagcttcctg
    25621 atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc caaaagcaac
    25681 gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta gatgaagcaa
    25741 cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat tacctgacag
    25801 aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag gcagataaaa
    25861 tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa ctgttagctc
    25921 aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct atgacaagac
    25981 ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    26041 gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg taactcagag
    26101 ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc tatcggcaat
    26161 aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc tgttgtttca
    26221 taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata cggttcaagt
    26281 cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt acttcttctg
    26341 attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg aactcttgtc
    26401 cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg aacagtttgt
    26461 gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg aattctggta
    26521 attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac atgttatgcc
    26581 ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt tcaaaggggc
    26641 tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc ctgaacgcct
    26701 tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc atattgattc
    26761 aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc aacttgatct
    26821 gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc tgtttttgaa
    26881 gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac atttccactt
    26941 gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt cgaaatcact
    27001 acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc gaaattagtt
    27061 tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc agccgctgtt
    27121 ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc ctttgatatg
    27181 acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga atcaacgatt
    27241 ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact gtaagcaccc
    27301 gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc ataatactta
    27361 tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt ttgtaaaccg
    27421 atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc ttgatacatc
    27481 ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact ttgcggttcg
    27541 tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata tacgcttaat
    27601 ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa tcctctaaag
    27661 acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg atctaaagct
    27721 actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa tgcactgttt
    27781 tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata gattttatag
    27841 cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc atcgatcact
    27901 gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt gtcattaaga
    27961 tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat ttttccgttt
    28021 tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg gtgatcgatc
    28081 ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc agacagcgta
    28141 acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc ccataccccg
    28201 ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct gtacttcttt
    28261 tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg gtctaatgtc
    28321 agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc tgatgttccg
    28381 ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa cccgtgatgc
    28441 tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg agaaatcgcg
    28501 tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc gtttttctta
    28561 tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt tgcttgtttt
    28621 gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat cagcaaaatc
    28681 aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa ttgtccgaga
    28741 tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc taaaatgctt
    28801 tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt ttaccatagt
    28861 gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac gttagtgcaa
    28921 gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat gttttaaaaa
    28981 tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc acgatcactt
    29041 tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa tggttagttc
    29101 ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat caacaacctg
    29161 ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc gaagaaacag
    29221 attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt ttatatgaag
    29281 atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca aatgaaccga
    29341 cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa cagcaacgac
    29401 cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt gcaaaaaatc
    29461 catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat cggagatatc
    29521 aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa attggacgag
    29581 atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc gccttgacac
    29641 atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt ttggaaaccg
    29701 gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc gttggagacc
    29761 ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa ctgcacatga
    29821 tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta tgggaataga
    29881 aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct ttttgtgtaa
    29941 tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga taatcccact
    30001 ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt ccatataacc
    30061 acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct gattcattac
    30121 cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga tcagtgcttg
    30181 agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat cttgcccttt
    30241 gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac aatctttatg
    30301 ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa tatgtcccat
    30361 ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg caccgattcc
    30421 tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt gcttcaattc
    30481 cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt agcgttcctt
    30541 gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca aaacgtatcc
    30601 gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc cgattccctt
    30661 caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg gtgttgtggt
    30721 agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca caaattttgt
    30781 tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat tgtccgaatc
    30841 tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag cagattcaat
    30901 atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact agacatattg
    30961 aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt aattgcaaac
    31021 gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc atgatgtttg
    31081 atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt agagaatttt
    31141 tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag acactgtgct
    31201 tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag tagcgtcact
    31261 gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc gactgttaca
    31321 ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt cgtggggttt
    31381 tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact atctgcttcg
    31441 tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc tttcatctga
    31501 ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt catgattgtt
    31561 tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg aaattccaca
    31621 accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc catttcctgt
    31681 agaatactta tttttgctat ttccggtgta tctactaata cttttacttc agagttttct
    31741 ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata ctcatttcct
    31801 atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt tttgcgatct
    31861 tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt ttcgatgatt
    31921 cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc ttctacaagc
    31981 gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac tttgatcatt
    32041 cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa ttggacagtt
    32101 tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg atacttacgt
    32161 ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc gaatcttttt
    32221 ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc agtatttaca
    32281 atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc ggtcagctgt
    32341 tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa acgaggtatc
    32401 atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg atgattttgc
    32461 attaatgtct tccaagcata atctaattga tcatggggat agtttccact aacttcataa
    32521 gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt ggtattccat
    32581 tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc atccgtttct
    32641 tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt ttgaactgct
    32701 tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag ttcatttgcg
    32761 agatggattg cttttgatat atctttttgt accggttgat gatcaacacc attcatcatc
    32821 aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt ccaaaaggca
    32881 agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt ggcaaatagc
    32941 agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta ttgggaagaa
    33001 tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc acggccaaac
    33061 gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat atttccaaat
    33121 gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc ttccatgcca
    33181 atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg caagatataa
    33241 aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg tcttttttct
    33301 ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg aaaactgttg
    33361 aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat cagtcgcatg
    33421 tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga aatgatatat
    33481 actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa gtaatccatg
    33541 actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata attttcagga
    33601 ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc tacaagtaaa
    33661 ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat agctaatgca
    33721 atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc gtactttcct
    33781 tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag ataatcttcc
    33841 tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac attactgtca
    33901 tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt ttgccctttt
    33961 tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt caatttctgt
    34021 gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc cacataacct
    34081 aacacgacca ccgcaaacat attggatggt accagatatc cataacggca agtatcatcg
    34141 ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc tcgcccgtca
    34201 ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg agagtgttca
    34261 tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat aaaagattga
    34321 ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg tattgggtag
    34381 cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt tgtatgatct
    34441 gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg atcgatattg
    34501 atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc ctcatcttct
    34561 tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca catcgctgga
    34621 atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt tgtcaacaga
    34681 gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc cccacatttt
    34741 tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata agccactaat
    34801 aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc caattatgtc
    34861 tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat tatttgaata
    34921 tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa tatttaatga
    34981 tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac caactgaaaa
    35041 agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat tgactgaatt
    35101 agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta aatccgcttc
    35161 tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc ttcttccata
    35221 tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga tgcaagataa
    35281 acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag caaaagagct
    35341 aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac atcttgactt
    35401 actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa aaatctatga
    35461 gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg ctgcctctta
    35521 tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga ctcatccaca
    35581 atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg gtttgacttt
    35641 ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt atatcactaa
    35701 gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga ccatgaatct
    35761 cttgagacag catggttatc atatccctga agatttttca attatcggat ttgatgatat
    35821 ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt ttgaacagtt
    35881 aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc gaccatccga
    35941 tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat aaaatgaata
    36001 tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct aatgggaact
    36061 gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa actacggatc
    36121 aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac cgtcttaccc
    36181 atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg atcaatggaa
    36241 aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa gaaagtatat
    36301 tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct acattgatac
    36361 ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc ggtttacttt
    36421 tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg gaaggaaaaa
    36481 tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata ttgagatttg
    36541 aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac tcgatcccgt
    36601 tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt atcagctcgg
    36661 aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact taccttgctc
    36721 acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac catgatcaac
    36781 aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca caaacatttt
    36841 atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga tctataaaac
    36901 ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt tatcctcttt
    36961 attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg aacagctata
    37021 gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt atgcctggga
    37081 cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga gacttgatgc
    37141 ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat ccaaattatg
    37201 gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac taccacgaat
    37261 cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa gttgccaagg
    37321 tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac tatcaaaata
    37381 tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag ttcagagaac
    37441 cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg caaagcagct
    37501 ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt gagagatata
    37561 aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga ggatatggtt
    37621 ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta gctatttcaa
    37681 accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct gaaatggcac
    37741 agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg ggttaccctg
    37801 gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg ggattttatc
    37861 ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac aaggctgttt
    37921 tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg ccgcagcaac
    37981 aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat tttacccatc
    38041 aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct caccctaaga
    38101 aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa gaggttgtga
    38161 aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc tcatattttt
    38221 cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat tgagaggttg
    38281 tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt tcctcatatt
    38341 ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta gtggagaggt
    38401 cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
    38461 tttttgcata aatttggctt aataccgaga aactggcttt tgaactccgt ttatcgagag
    38521 gtcatgaaaa agctgccttg cctcaagtaa taagaacagc ccaaaattag atttctagac
    38581 aattttttat aagttatttc attacattgg ctttttcttt tatgattctc cactgttttc
    38641 tcggtactct ttaggggatc tgccgacgat tttcttgaat aatttagaga aatacgtggt
    38701 gttgttgtag ccaatctcac ttgctatctc attgatgttt tgattactgt gcaaaagcag
    38761 ttgtttcgct ttttccatac gtaaatggtt caaataagct gaaaaacttt ttttcgtttc
    38821 ttttttgaac agctgtccta gatacatgac atttaaatgc aggttttctg acacttcttt
    38881 gagtgtaagt ggttcttgat aatgctggtg caggattacg attacttttt ccacattgtc
    38941 actgtacttt ttctgtctct gatgcttttt gatgaatgac agaagtagct gctgaagttc
    39001 ttgcaccgtt tttgcctgat ggattttttc gattccttgt aaatattcct catcttccag
    39061 atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa ctggaatatc gtatatcttc
    39121 cggcatcatg gctgctaact gaaactgttc aaaaaaatca gtgatcattt tttgagcgac
    39181 ttccaattgt ttattttgca gcacgcgtct gaaacttgaa aaatcgatgg tttgatcttt
    39241 tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta tctccataaa attgatgcaa
    39301 ctgtagattg tctttcgctt gttggaatgt ttcagggatt ttgtccaaag aatctgtctc
    39361 ttccccgata ctgatcagcc attcctgatc aggaaagttt tcttgaacaa aacaacagaa
    39421 gccatcaact tcatctgcct ccaacaacaa cgtgatcaat acacggtctc cgtaatttcg
    39481 ttgatagtaa aagatttgtt gccgttcttg caaccatcta ttgataagag tatcatgagt
    39541 tcttgccagt tgaatcaata ctatcctatg ctgcttctca cttatttgtc ctattatttc
    39601 ttcttcactt atttcatcca attcttcatt caaccattga ctgaataaca gttcttgata
    39661 gatttcttgt tgattttttt gttcattttg ctgatctaac cgtgttttga ttttcttgag
    39721 gctttcaacc aattcgaact tattcacggg tttcatcaaa taattcaagg cgcctaattg
    39781 catacctcct ttcaaataat cgaattcttg gtatcctgat aaaatcataa attcaaaatt
    39841 atgacgttct ttttgagccg cttcaataaa ttccagtcca tttatttctg gcattgtcac
    39901 atccgttact actaaatcaa tctgtcgctg ttcaagtaca gctagtcctt ctttggcatt
    39961 ctctgctgtt gctactattt gaaagcctaa actttgccaa tcaattatct tttttaaccc
    40021 attcaaaatc atgtattcat catctaccaa taacacttga tacacgctca tccctccttg
    40081 aatgtaatag aaacggtcag tccacctgtc tcattattct caagcgacat ataataactg
    40141 ctgccgaagc tggcacgtaa acgttcattc acgttttgca gaccgataga accgtgaagt
    40201 tccactttag gatgagacag tttttcttca atctgtttta gccgtttttc agtcatccct
    40261 tttccattat ctttgatgat gatccttacc cgtttgcctt cctgcaagac tttgacactt
    40321 agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa aataattttc aactaatggc
    40381 tggatcacaa atttaggtac ttcgatcttt tccaaatcag gatcgatcat aaaatgatag
    40441 gctactcgat taggataacg catttgatac aaatagacat atttttcgca aaagtccagt
    40501 tcttctttta aagtgatcgt tttctcttga ttcgtattgt ttcgaagcaa agcggaaaac
    40561 gcatacacaa catctgctaa ttcttcactt ccttcgctga ttgcatacat tcggatatat
    40621 tctaatgtat tatataaaaa gtgtggattg atctgtgctt gaagcgcccg catatgtgcg
    40681 tcttgctgtt tgatttctaa tttgtagatg tcttctacaa actgattgat gctatccaac
    40741 atttcgttga taccgattga aagttccttc agttcatatt ccgtgtttgt tatgtcgatc
    40801 cgcgtctcta aattccctgc tgctgtttga tccatggcct gcatgacgat agatacttgc
    40861 tgtgaatatc gtttgaatgt tttataaaga aagataagca gtatattgtc taaaataatg
    40921 ataccaatga tcagcggggc aagatttttt cccgtttcga tttttacact ttttttatct
    40981 agtacagcta atatacgata atcatcagac agttcttggt gttgaacaaa attgtttttt
    41041 tccagatcgt caatcggcaa ttgtgaattt ttcttcagac tctctgtgat gatcatttct
    41101 tgtctttcaa acgtttcttt agctatttta ttatcatgga atgtgtatag tcgatttgtc
    41161 gttccagaaa tcatgtataa agagagtcca ttgaatgacg ttagattttc taaaatcata
    41221 tctagttctt tcttttgaaa accaataaaa atacttcctg actcagctcc tccttgtgtg
    41281 ataggcaact tgatataaaa ggcgtctagt aattttggtg tccccttttt tatttcccca
    41341 cctttgtttt ctctagaaga ttcataatac tccggtagtt gattcaacac gatatatatc
    41401 gcacttagtt cttcataatt tgcgtagaga tctttgattt gatttgggaa agaaactaat
    41461 tcatcggtac tgttttgctg ttcgtataca tacatcagat actgatctat gggttttgtt
    41521 aagtaggtcg tcacattttc gatcttttcg gaattcccta caagctggtt tttgatgatc
    41581 ctactcaagg ttgttttatc attcagcatt ctgcttgtgc tttgaacggc atcacttgtc
    41641 atgttttctg cctgtttttg attttgttcc caagtatgga tactaaaaat aacggtagca
    41701 gtcatcgtta caccgatcat gataaccgcg tatttccgca acagacgatt tagtaaggtt
    41761 ccttttgaaa atagaatccg aaacttattc ataactttcc aactgtttat ctaattcttc
    41821 acgccaatgt gttgtcatcg taccattcca aaccgtcaaa agaccaatga caccaaacag
    41881 gatcaatcct ttgaattgcc aagtcacgcc taaaattata ccgctaccaa taattatttt
    41941 tagaaaagac gaaaagctgg aaaaaaccga aataaaactt agctttagca gattaggaac
    42001 agagatcgta tattcactat ctaaaatcat actgtattga taagtagcaa ataggcagag
    42061 caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg agccctttga tttgaacaga
    42121 taaatataaa ttgtaactca ataaagcagt accagataaa aataaactaa atagcagatt
    42181 gcctcttaaa aaatttcttt tatatatatt ccagcctctt tttattgtga tttctttata
    42241 ctcgaatccg tacaaataaa atagttcatt gactgttttc catgccgcac ctaatccaaa
    42301 gatgatcccg ccggcaaatg tatatatcca gaaatacaag gttagtttta taatgaccca
    42361 gatccggata aataactctt ccaaaacctt ccctaacata tcttctcctc caaacattaa
    42421 gtttatactt tctcattata caaaaaaagg ttgggcaatc agcaccttga cctgattatt
    42481 agagaaacaa acggaaaaat agttcctcat attttgtcgt tttgtgagcg aataccgagg
    42541 gttagctgat tgaacaccgt ttaccaagag tttgtgaaat agccgcttag tttcgagtag
    42601 taagaaaaat tttgtgaata tggctttcca catttttaca tatatttagc ttaataccga
    42661 gaaactggct tttgaacacc gtttattcgg aacaagaggt tgtgacacgt cttttgtcac
    42721 aacccctttg tctttattca ttattattct tgtcggaact catcatattg tttttgcatt
    42781 tctttcaata ccttatcata gccagccttg tccaaagcgt ctttcaattt cggtaatgtt
    42841 tcatctggat caacagtccc tgtattcaat ccatctaaat attgattcat gacattagaa
    42901 atattactta attctgtttt gacactgtca gtgttgaagc tgaatcctaa aatcggtgaa
    42961 gtttctgcat cagcaatcga ttgatcacgt ttggcaatca tgtcatccgt aatagactct
    43021 tgtgtatata gaatcttgtt gtttcctgta ttccaagcag acatatgtgt atttggctga
    43081 taaccatcta gcaatttgat tttatcatct gaaccatcga ctttttccca tgcttctccc
    43141 tcgatccccc aaacaagccc attcaataat tctggatcac tgttaagcag ccctagaagt
    43201 tctactgatt tttctttatt ttttgaagta tttgatacga caaaatttgc catttgtgct
    43261 tgagctgtag attttagtgg aacagtaatt gcttttgaga caagttcttg ttttgctgca
    43321 tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg tttctccacg cattagccaa
    43381 gtatttcctt ctagtggata atccttattg ctcgttgcag cgtcacttgg aatgagtcct
    43441 tgtttgtacc agctatgcat tgtacgtaac agtccgatat acgtttcatt atcgtattgg
    43501 ttgatgattt tcgaatcatc tccatccaga tcaatagcaa atggtaacgt attccctaaa
    43561 ggataatcaa agttcccttg gactttgaag ccttgtccaa tggcaaaagc tgccgtgttt
    43621 ggttcttttt catggaattc cttcaagact ttttctgcat cagcgtaaga ttggattcca
    43681 tcaattgaaa gatcgtattt atctagtaaa gctttgttaa aagtaagtac ttgttgagca
    43741 aagacatttc cattgactgg gaaagcatat aattttccat tgaccagatt tcctttaata
    43801 tatgcttcat ctaaatcatt ataagctttt tcagcgtact taggcgctaa ttcagtcaaa
    43861 tcagcaaagg ctcctttttg ggcattggaa acatagttgt tcgcaaaggc gatatcataa
    43921 ttttccccgg aagaaatgat gacgttcatt ttcttttcgt aatcgcccca gccaatgtaa
    43981 ttgatattga cttttacacc gatctctttt tcgattcgtt tgtttgctac ttccatcaat
    44041 tgatcgaaat tatctggttt atctccgatt tgatacatct gcaaagtgtc cgaattgcta
    44101 gcttctgcct ggtctttaga accagaacct ccgcatgctg ctaatgttcc caacaacaac
    44161 gctgaaacac ctgtaaaggc tagtttcttc cataacttca tttttcttcc tcctaatttt
    44221 atgatttata tttttgtttc tttattcttt tactccgccg attgttagac ctttgacaaa
    44281 atgcttctgg aaaaatggat agctgatcgc aataggcaac gtcgagatga ctacgatcgc
    44341 cattctggca gattctcctg gaactgaggc taatccgcct gtcaattggc ttcctgctcc
    44401 tgaattttgt gtcagatatt gaatattact ttggattttc atcaaaagat actgtagtgg
    44461 aactagctta tcatcttgga tgtatagtaa agcattgaac cagtcattcc agtaacccaa
    44521 tgcggcaaat aaactgattg tcgcaatacc tggaacagct aacggtaaaa cgatctgaac
    44581 aaatattcgc agttcgctag ctccatcgat ccgtgccgat tcaataatcg aatcagagac
    44641 cgaacgttta aagaatgtgc gcatcacaat aatattgaag ggactgacag ccattggcaa
    44701 gatcaatgcc caaatcgtat cttttaactg aagaagattt gtcataacaa gataattggc
    44761 caccatccct ggtgagaaca acatcgtaat caaacaaaaa acggtaaaaa aacgacggaa
    44821 tggaaagttt gaccgtgaaa tcgcataagc atataaagaa gtagccgtgc tattgatcag
    44881 tgtacctaaa acagtaacaa gtacagtgac gaaaagcgct tggaaaattt tatcctgcat
    44941 ctggtcgaac aagtacgtat aaccaaatat actgaacttt tctggccaaa aactgtaccc
    45001 gtgagtcgac aatatggact catcggtaaa agagatggcg ataacaaata taaatggcaa
    45061 aatacaggaa atagcaaaga gtgcaatcat tatattagag aaaaaattag ccgtcttatt
    45121 gaaagagcgg acttggactc tctccacttt cttttttttc atttttttcc tccttagaat
    45181 aatgcagaat cgctatcaaa acgtcgggcg atcgtatttg tgatcatcaa caagacaaag
    45241 ccgacaactg attggtaaag tcctgcagca gccgtcatac caaaatctcc tgtggatgtc
    45301 aggccattgt agatatacgt atccaaaact tgagtcacat catataatgc accggaattc
    45361 ctaggaatgt tataaaacag gccaaaatca gctcggaaaa tgtttcctac ggctaaaatc
    45421 gttaaaatcg tcattagtgg aataagttgt ggtatcgtca catgtttgat ctgctgccat
    45481 ttgctagctc catcaaccat agcagcttca taataagtcg gatcgattcc cataacagat
    45541 gcaaagtaaa taatgctgtt ataaccaatc cctttccatg taccaatgaa caccaagata
    45601 aacggccaat atttcggttc attataccaa ttgacagctt ctcctccatg tccagtgatc
    45661 cactgattga atattccttt gtcaggactt aaaaaagcgt aaacaaagta actgataatg
    45721 acccaagata gaaaataagg tagtaaggac atcgtttgat aaactttaac gagtcgttta
    45781 tttctcaact cgctcataat gattgcaaat acgactgaga tcagcagatt caaaaggata
    45841 aatgctacat tatataaaat tgtattttta gtgatgagaa atgcttgatc cgaagaaaat
    45901 aaaaatttga aattttccca acccacccaa ggactttccc tcaagctagc caagaaccca
    45961 tctgctgaaa tatgaaaatt tttgaatgct acgacattcg ccattacagg tatgtaaaag
    46021 aaaaaaatca tccaaagcat acccggtaca gccatcagga caagtgcttt ataacgccat
    46081 agattcataa aaaaatgatt gaaccccttt cgttttttca tctaaaaacc cctttcgatt
    46141 cattcgcaac gtaattatag cgctttcaat aatgagaata aataaacaaa gtatagggaa
    46201 aatgaaacaa agtataggag aggttgtgaa atcagcgttt tggcctgagt attagagaaa
    46261 aaaacgggaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagct
    46321 gattgaacac cgtttatcag aggttgtgaa ttcagcgttt tggcctgagt attagggaga
    46381 tcgtgtcctt actatctaac tctgagtcac aaggaacaat ggggactgta acaaaagtct
    46441 tgtcacagtc ccttattccg aacaaacggt gttccagaag tagcttctac ggaaataagc
    46501 cgaaatttca caaaaaagcc atccgaaagt cagacggaca agtctgcttt tcagatggcg
    46561 ctacaactac tctgcttatt cttttagcta tccattcaat tatttttctc tcacttgttc
    46621 tagctcttct ttggtcatgg gtcgatattg ccctttattc aaatctttag gcagccaaaa
    46681 ttttcccata cggattcttt ttaaatataa gacttctttt cctactgctt tgaccattcg
    46741 cttaacttgg tgaaactttc cttcatgcaa aatcagacgg ataattgaag tttctgtctc
    46801 ttgatctact tgatcaattt ggagttttgc cggcaatgtc tgctcatcgc catcaataat
    46861 cagtccttct gcaaataaac ggatatcttc ttctgtcatt ataccgctaa cctcagctag
    46921 atattctttc tccacatgtt ttttaggtga tagtaactga tgggcaaaca ccccgtcatt
    46981 cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc
    47041 ccgataatcc tggtcttcca ataaatcgat tacggtttga tcatgattat cctgagtagc
    47101 agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat
    47161 ttctcctaaa taagtaaccg catcttcgaa ttcatttact tgcgctttat cgcttttcac
    47221 cacttgtcca ttgacttcga tcgttccttt tttgattaat cctttgactt ctttgcggct
    47281 gccaagtccc atatctgcta aaaacttatc taaacgcatg cgtttccccc atttcaaaag
    47341 agcatcaatc tagctcttct gattcactta ttttattttt aatttatgtc gtaaacgcgc
    47401 tattcctggt cctagtagtc tttctgccaa gcggattttc aaagctaaat aaccatacac
    47461 taaagcccct atacctgcta ctacaagaat caacaataga gactggaatt tgctatcttc
    47521 gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa gccaaaagca taatcaaggt
    47581 aagaataaag atcagcaatg tcctgcgcca gacaaaactt ggtttaaagc gggctacttc
    47641 atggattctc tttaagatca aggtacaaga cacagcaaat ccgatcattg tcgccaataa
    47701 agggccgtat acttcaaata aacgaatggc tgggtattga agaaggagtt tcactagaaa
    47761 cccaataacc aaataattaa ttgccgcctt gttttcaaac attccttgca gcatgttgga
    47821 aactagcata tatagtccta ggaagagtcc tacaaaactt gcttggatca gtacattact
    47881 tcctagagaa tccggtgtat aaaataatgt atttagcgga taggcaagta acataacacc
    47941 aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc
    48001 cagacctact cgatctttca acgtaacagc ttctgtaatc aacggtaacc cagtagacgc
    48061 gatcgatgtt gccaatgcaa taactaccat cgtcaactta tctggattgg cactaaaaat
    48121 cgaaaaaaga tccagtaact gtgcattgga atactcggtg gatgcagaca tgatcttcat
    48181 aaaagtgaac tgatcgacta atttgaagat cgttacccct gaacccacaa taataaacgg
    48241 aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt gcaatattta ttttgttttc
    48301 gctgtagtga ataaacgccg atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac
    48361 aagaatactt gctagcatac cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac
    48421 atagtcacca tccatgactt tcatgatgat gaatgtagcc aatagcatat aaaaaatacg
    48481 tgccacctgt tctacaatct gcgacagcgc ataaggcatc atttcttgat tgccttgcaa
    48541 ataccctcgt atcacactca tacaaggaaa aaccaataca gcgatactca aagaacgcat
    48601 cgttggaatc agttcttctc cgccaccaga agcatgagct agccaaggag aggcgatgta
    48661 catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc tgtaatgcac ggataaataa
    48721 tcgtcgtgaa gtgccatatt cattcaatga attatagcgt gcagtttgtt ttgcaatcgc
    48781 agcaggtatt cctgctgtag agatcagtag aaacaatgca tatatattgt atcccatatt
    48841 gaataatcca ttggcagcct tagcattttc gcccatccaa gcataccagg gaatgatata
    48901 gacagcgcct agcaatcttg aaatgatatt actggcggtc atccaagcag agcctcgtgc
    48961 cattttttct tgcggtgtta attgatctaa tgctggtttt ctttgattat ccatcggtca
    49021 gattccaact ttctacaaaa cattactatc attttaattg gtaaaacaga aactggcaat
    49081 cgattttata taacttttat aagaacaatt gtttttttga attcggtttc gtttttttgg
    49141 taaagtaaag aaatatacag ccttttatgg ctaacattta taggagatat caatgacaca
    49201 agaaataaaa aaactacccg ttatattttt aggtcttttc tttatcacag ttggattgaa
    49261 ttggttcttg ctccctcacg atatagcttc cgccggagtg ggcgccatcg gacatttagt
    49321 tgaaacggcc ttttctatcc aacgctcagt tactgtttgg agcatcaatc ttatgatgct
    49381 gcttctagcc gctgtcctat taggaaaatc agtatttttt aagactgtag cgggaagtct
    49441 gctttttcct gttgttttag agattgttcc aaaagtggaa atgatctcct cacatttctt
    49501 ttcactgatt tcaggaagtc ttttgttcag tcttggggtt tacacattgt acacagtcgg
    49561 ggcctcaaat ggcggtataa cgataccgcc gattattttc cacaaatttt ttcggcttcc
    49621 aatgccgcaa ggacttttac ttaccaatag tcttatcgtc tttttgaatt attatgtttt
    49681 tggattgttg gagacactat tcgttctcct atctattaca gtcagttcat tgtttatgaa
    49741 aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata
    49801 gcggaaacaa acgatatatt aaaaagagtg tgctataata aaccaagaaa ttgaataaag
    49861 cgagggatca atcaatgagc ttctctttag aagacatccg tcatttacta ttaaaagaaa
    49921 atctattaaa agaatttgtt tcaacaagag gctggcatct tgatgtacca gaaaaagctg
    49981 aatttcttca gttaagttat gattcaagaa aagctgatgc ttctactttg tttttctgta
    50041 aaggaatgaa tttcaaagaa gaatatttgg attcagcaat cgaacagggt attcaatatt
    50101 atgtatcaga acaaccatac gaaaattcag cagtaggaat catcgtaacg gatatccgta
    50161 aagcaatggc gcttcttgct atggcttttt acgactatcc gcaaaataaa ttgaaagtga
    50221 tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg
    50281 atcacacaac aaataaaaaa acagcattgc tttctactat gaatacaacg ttagatggca
    50341 aaacatattt caaatcccat ttgacaacgc ctgagtcatt agatttatat cgtatgatgt
    50401 cagaagcagt tgaaaatggt atgactcatc tagtgatgga agtttcttca caagcttaca
    50461 aaacacaacg cgtatacggc ttgactttag atgtcggaat cttcttgaat atctcgcctg
    50521 accatatcag tccgatcgaa catccgacat ttgatgacta tttctattgc aagcgacaat
    50581 tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag tgactatttt gatttactta
    50641 gggaaacagc cgatctattc cagattccaa cgatcactta tggtcgttcg gaagaaagca
    50701 attatcaagt gatccgttca gataaaggta ctcacggttt tactttatct tctcatgaag
    50761 atcgcttagc tatctgcgat actgcttatg atattttgct ggctggagga ttcaaccaag
    50821 aaaatgcagc cagtgcgatc attgccgcag cactaacagg tgcctccagt caagatgcac
    50881 aagacggatt aaaagaagct cgtgtaccag gccgaatgga tcaattgatc caatccaatg
    50941 gagcgcatgt ctatgtggac tatgcacata attatctaag cttgaaaaca ttgctgaaat
    51001 ttgccaaaaa tgaacatcca gatggccgtg taattgttgt attaggcagc ccaggaaaca
    51061 aagcaatctc ccgtcgacat gattttggaa aagtgctatc agaaacagct gacatcgcct
    51121 ttttgacggc agatgaccca gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg
    51181 aagcaattac taatccagat ttgatcgttc attatgaaat ggatcgtcct gaagcgattc
    51241 gacgcgcttt agcagaaagc gctccgcaag attcagtcgt tattgcggga aaaggcgtgg
    51301 atccctatca aaaaatcaat ggtgtagatg aaccttatga aggggattat gcaatcgtga
    51361 agcggttgat tgaagaataa tggtatttat atactagtgg tttcccattt ctaagaaaaa
    51421 aagggactgt gattttttgt cacagttcct tttttcttag aaatcatttt aacttttcac
    51481 tagctaattc tgctgccttt tctctttctt catttaataa tttattatca tagaatttga
    51541 taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac acagcagctt
    51601 tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc cacggcatct
    51661 gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt gtagcagaag
    51721 ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt ggcatcccga
    51781 aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt cctaacactt
    51841 tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa gtcgcacctg
    51901 atccaccaac ggttacataa gaattgttga attctcctgc caacggataa tttgcgcctt
    51961 gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg atgatattcg
    52021 ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc atgacaccaa
    52081 gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt ttgaagatat
    52141 ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca atgacaaatg
    52201 ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg aacaacatct ggcattttta
    52261 tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc gacataataa
    52321 tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc ctaataccat
    52381 ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca tcagtcatga
    52441 tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc gtgagcggat
    52501 taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca aacacaatgg
    52561 caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac aagtcactaa
    52621 ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat
    52681 ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc cccgctgcca
    52741 tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc aggtatttct
    52801 gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt catattgttt
    52861 aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat actgtataaa
    52921 tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc ctccattgtt
    52981 atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg gaatcactaa
    53041 taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt tcgtttggtg
    53101 ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa tcagaatcat
    53161 cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt gcatgttcat
    53221 gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat acatgttttt
    53281 caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct gcacaatgat
    53341 ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa cttccaataa
    53401 tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca tttctatttt
    53461 tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat gaagaatctc
    53521 ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac gcatcgagcg
    53581 aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat caaaagaagt
    53641 cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt attcgatcag
    53701 tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt cctgctgtac
    53761 ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc cttcattttc
    53821 gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat cccactgcat
    53881 cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa cagcgggatg
    53941 tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt gctggatctg
    54001 ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt gcagcaaatc
    54061 atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa
    54121 ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg gtctatctga
    54181 ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat catcttcatt
    54241 caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa gatcaataga
    54301 agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat agccttttac
    54361 acttaccact gtaatatttt taccgaattc actgactacg cgattcttta atagttgggc
    54421 actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag ctctttcttt
    54481 ttccaatgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat tgatggtata
    54541 gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga ctttgggatt
    54601 ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat
    54661 catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct ctactaatga
    54721 aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg atagttcctg
    54781 atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca aataagcaat
    54841 ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga tccgctgcgc
    54901 cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct tgatttcaaa
    54961 cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct gtatgataaa
    55021 atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga ctagctcttc
    55081 aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg aatttccata
    55141 atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt ccccttcgat
    55201 ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca atcgctcctt
    55261 gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt ctgccaaatc
    55321 atccataaaa agaatggcat cttccaaaag caattggttc aatatatact tttgtcgatc
    55381 ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt cttgccgact
    55441 caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat aacctggttt
    55501 agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca agtaattacg
    55561 gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga catactcctt
    55621 cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta tcatccactc
    55681 ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc tgtagggatt
    55741 gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc tcctaatttt
    55801 ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc aaaatcacta
    55861 ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac ctcttctctt
    55921 ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat tcctgccgca
    55981 caaataatca atgctttttt cataaatcaa tcactccttt tatttttata aaccaagcat
    56041 atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga aaaaatgtgt
    56101 atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa ttttttcagg
    56161 aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg gacaagccag
    56221 aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg
    56281 aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt tactgatcga
    56341 ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat
    56401 aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca ctgatttacc
    56461 gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt cactttttcc
    56521 ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta tttttcggat
    56581 aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga tattctcaaa
    56641 aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg ccaaagcacc
    56701 aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag ctagaccacc
    56761 taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata aaacacatcc
    56821 aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg acatcaatcc
    56881 atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac
    56941 atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca tccaaatttt
    57001 ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt
    57061 ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa ataataagct
    57121 acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa
    57181 gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag cggttgccat
    57241 taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc atttgatcag
    57301 ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta tgatcgaatg
    57361 ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga ataaaaccac
    57421 agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa aacaaatggc
    57481 aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc acttttcttt
    57541 tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt gtagagactg
    57601 ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag tcatctatga
    57661 caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca actatttttc
    57721 ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc accacaaaat
    57781 aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt ctgagatcaa
    57841 tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag aaaaaaacat
    57901 tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt ggttctgctg
    57961 tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc
    58021 actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag cgctatacct
    58081 gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca aggcactcga
    58141 tttcaaatca tgtcagacat aaaatcactt ctttactgac tgaaaaagtg tcgttatttt
    58201 ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg aaagagttga
    58261 gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt
    58321 tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg gcgagacaga
    58381 aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa ctatttcctt
    58441 agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc cacctacttt
    58501 cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt acttggtttt
    58561 tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct ttccgagaca
    58621 aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag agaaaaaatt
    58681 acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat aaacttgagt
    58741 cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt tgtatatttt
    58801 ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc acaaaattgt
    58861 ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata tttgtttgta
    58921 taccccttct aaaatcggta gtttccattc aattgatggg tactagttga ttttaaaaac
    58981 tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa attttatact
    59041 gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga tggtttctcc
    59101 agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta tatctgagct
    59161 atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt ctttttcgat
    59221 cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag tttctaccag
    59281 ctgatatgct cctggtgcta agccatctat cgccagtttt ccatctgctc ctgtggttag
    59341 acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc cttgcaggac
    59401 ttctccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa gtcgattttc
    59461 tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga ttggtgtcgc
    59521 atccaattca taccccgttg gcgcttgggt ttctaccagc tgatacactc ctggtgctaa
    59581 gccgtcaatc gccagttttc catctgctcc tgtggttaga cctgactgca gaacggttcc
    59641 attggcatct tgtaactcaa aaacagctcc ttgcaggact tccccgcttt ggtcatcgat
    59701 ctttgtcaac acgacaccgc ctggtgtaag tcggtttgct ttcgtcaatt ctaccaccgc
    59761 tgtctgactt ctttcgatct caaactcgat cggtgtcgca tctaattcat acccc
//
LOCUS       DACDHL010000008        59619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDHL010000008 DACDHL010000000
VERSION     DACDHL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.008"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            189..419
                     /locus_tag="IV228_000514"
     CDS             189..419
                     /locus_tag="IV228_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8716455.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="IV228_000515"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="IV228_000515"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8716456.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="IV228_000516"
     CDS             1830..2006
                     /locus_tag="IV228_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8716457.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="IV228_000517"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="IV228_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8716458.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="IV228_000518"
     CDS             2622..3089
                     /locus_tag="IV228_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716459.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="IV228_000519"
     CDS             3174..4058
                     /locus_tag="IV228_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8716460.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4175..4408
                     /locus_tag="IV228_000520"
     CDS             4175..4408
                     /locus_tag="IV228_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8716461.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="IV228_000521"
     tRNA            4496..4576
                     /locus_tag="IV228_000521"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="IV228_000522"
     tRNA            4583..4654
                     /locus_tag="IV228_000522"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            5169..5876
                     /locus_tag="IV228_000523"
     CDS             5169..5876
                     /locus_tag="IV228_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8716462.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="IV228_000524"
     CDS             complement(5935..6579)
                     /locus_tag="IV228_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8716463.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="IV228_000525"
     CDS             6705..6992
                     /locus_tag="IV228_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716464.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="IV228_000526"
     CDS             7100..7420
                     /locus_tag="IV228_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8716465.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="IV228_000527"
     CDS             7501..8814
                     /locus_tag="IV228_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8716466.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="IV228_000528"
     CDS             8811..9530
                     /locus_tag="IV228_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8716467.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="IV228_000529"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="IV228_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8716468.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="IV228_000530"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="IV228_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8716469.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="IV228_000531"
     CDS             join(13091..13153,13155..14192)
                     /gene="prfB"
                     /locus_tag="IV228_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8716470.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="IV228_000532"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="IV228_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8716471.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14967..15860
                     /locus_tag="IV228_000533"
     CDS             14967..15860
                     /locus_tag="IV228_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716472.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="IV228_000534"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="IV228_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8716473.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="IV228_000535"
     CDS             17797..18654
                     /locus_tag="IV228_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8716474.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="IV228_000536"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="IV228_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8716475.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="IV228_000537"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="IV228_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8716476.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="IV228_000538"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="IV228_000538"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8716477.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /locus_tag="IV228_000539"
     CDS             21410..22168
                     /locus_tag="IV228_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8716478.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="IV228_000540"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="IV228_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8716479.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23095..24126
                     /locus_tag="IV228_000541"
     CDS             23095..24126
                     /locus_tag="IV228_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8716480.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24123..24890
                     /locus_tag="IV228_000542"
     CDS             24123..24890
                     /locus_tag="IV228_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8716481.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24990..26558
                     /gene="liaX"
                     /locus_tag="IV228_000543"
     CDS             24990..26558
                     /gene="liaX"
                     /locus_tag="IV228_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8716482.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26858..27175
                     /locus_tag="IV228_000544"
     CDS             26858..27175
                     /locus_tag="IV228_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8716483.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27175..27525
                     /locus_tag="IV228_000545"
     CDS             27175..27525
                     /locus_tag="IV228_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8716484.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27692..28630
                     /locus_tag="IV228_000546"
     CDS             27692..28630
                     /locus_tag="IV228_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8716485.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28645..29475
                     /locus_tag="IV228_000547"
     CDS             28645..29475
                     /locus_tag="IV228_000547"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8716486.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29519..30544
                     /locus_tag="IV228_000548"
     CDS             29519..30544
                     /locus_tag="IV228_000548"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8716487.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30559..31497
                     /gene="galU"
                     /locus_tag="IV228_000549"
     CDS             30559..31497
                     /gene="galU"
                     /locus_tag="IV228_000549"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8716488.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31622..32062
                     /locus_tag="IV228_000550"
     CDS             31622..32062
                     /locus_tag="IV228_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8716489.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32065..32667
                     /locus_tag="IV228_000551"
     CDS             32065..32667
                     /locus_tag="IV228_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8716490.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32726..33832)
                     /locus_tag="IV228_000552"
     CDS             complement(32726..33832)
                     /locus_tag="IV228_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8716491.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34042..35043
                     /gene="ccpA"
                     /locus_tag="IV228_000553"
     CDS             34042..35043
                     /gene="ccpA"
                     /locus_tag="IV228_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8716492.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35380..37725)
                     /locus_tag="IV228_000554"
     CDS             complement(35380..37725)
                     /locus_tag="IV228_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8716493.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38004..38906
                     /locus_tag="IV228_000555"
     CDS             38004..38906
                     /locus_tag="IV228_000555"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8716494.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39119..40894
                     /locus_tag="IV228_000556"
     CDS             39119..40894
                     /locus_tag="IV228_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8716495.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40960..42546)
                     /locus_tag="IV228_000557"
     CDS             complement(40960..42546)
                     /locus_tag="IV228_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8716496.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42797..43021
                     /locus_tag="IV228_000558"
     CDS             42797..43021
                     /locus_tag="IV228_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8716497.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43163..44914
                     /locus_tag="IV228_000559"
     CDS             43163..44914
                     /locus_tag="IV228_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8716498.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44914..46698
                     /locus_tag="IV228_000560"
     CDS             44914..46698
                     /locus_tag="IV228_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716499.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47024..47875
                     /locus_tag="IV228_000561"
     CDS             47024..47875
                     /locus_tag="IV228_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8716500.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47929..49680
                     /locus_tag="IV228_000562"
     CDS             47929..49680
                     /locus_tag="IV228_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8716501.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49684..49854
                     /locus_tag="IV228_000563"
     CDS             49684..49854
                     /locus_tag="IV228_000563"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716502.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49894..50796)
                     /locus_tag="IV228_000564"
     CDS             complement(49894..50796)
                     /locus_tag="IV228_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8716503.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51027..52430
                     /locus_tag="IV228_000565"
     CDS             51027..52430
                     /locus_tag="IV228_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8716504.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52430..53863
                     /gene="ascB"
                     /locus_tag="IV228_000566"
     CDS             52430..53863
                     /gene="ascB"
                     /locus_tag="IV228_000566"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8716505.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            54008..54934
                     /gene="trxB"
                     /locus_tag="IV228_000567"
     CDS             54008..54934
                     /gene="trxB"
                     /locus_tag="IV228_000567"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8716506.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55148..56875
                     /locus_tag="IV228_000568"
     CDS             55148..56875
                     /locus_tag="IV228_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8716507.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56947..57240
                     /locus_tag="IV228_000569"
     CDS             56947..57240
                     /locus_tag="IV228_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8716508.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57332..58192)
                     /locus_tag="IV228_000570"
     CDS             complement(57332..58192)
                     /locus_tag="IV228_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8716509.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58284..58712
                     /locus_tag="IV228_000571"
     CDS             58284..58712
                     /locus_tag="IV228_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8716510.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58874..59050
                     /locus_tag="IV228_000572"
     CDS             58874..59050
                     /locus_tag="IV228_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8716511.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59077..59523
                     /locus_tag="IV228_000573"
     CDS             59077..59523
                     /locus_tag="IV228_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8716512.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        19420 a        10339 c        12778 g        17082 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaagcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgac gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagtttc aggaggtctc tttgacttag atcagctgga agaagacatc gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgacagcga agccgcacaa aaagtgatca
    13261 gtgaaaataa cgctaataaa gaaacctatg accaattcca ttctcttgcc gaagaactag
    13321 aagaaatcga attgatggtg gagatgctgc aagaagaacc tgatgcagca atgcaagaag
    13381 aagcagaaac acgtatcaat gcattagatg aaaaaatgaa gacgtatgaa ttatctatgc
    13441 tgttagacgg accttatgat cgaaacaatg cgatcgtcga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagactgg ggcagcatgc ttttacgtat gtatacacgt tgggcagagc
    13561 agcatggcta tcaagtagaa acactagatt atcaagcagg tgatgaagca gggatcaaga
    13621 gtgtgaccct gctgattaaa ggacataacg cttttggtta tctaaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacggatt tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gctcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatcccta ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct cgattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gagatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tcgtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacgttgact ttagtcggtg tattcatggc agttatcatg aatgcaacta
    15121 aattagctca ggatatagaa ggaaacgttg atgtatctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagtaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaggaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat ggctgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaagcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaaatc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatatctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctgatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactgatt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcatttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacacaaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgaggattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgtg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta ttgttgccac accatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggacag ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta
    19321 aaggcagtac cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggacttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatgtagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctgacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcattgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggctttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggtttca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgatcat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcggctcg tatcagcgac tatactgcct ttttctatct
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 aattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagt ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cgggagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaaaat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 taaagcggtg attctgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa atagctcctc atatttttca
    22981 tttggctgga cttattaccg cagatgcaag cttttgaaca ctgtttattc gggaaagggt
    23041 ttgtgacaaa atatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa
    23101 aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag
    23161 catcaagctg ctttatatga agtacctgtc atcgagatcg aaaaagtacg aataaaagag
    23221 gatcatcaat tgattacagg aagactgatg aacacgaatc aaaaaggaga atcgatttat
    23281 ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg
    23341 tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg
    23401 cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggcgg aaaacatggt
    23461 ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc
    23521 tttactaaaa tagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg
    23581 atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca
    23641 gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatctttgg
    23701 aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt
    23761 tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc
    23821 attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg
    23881 ctttcgggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc
    23941 gtctatatca gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc
    24001 aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta
    24061 ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa
    24121 cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg
    24181 gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcttgt
    24241 tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat
    24301 tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt
    24361 ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt
    24421 tagcaatcca aggttttaca acgatagaac tagaagaatt atctggctta gataagactt
    24481 tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg
    24541 ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc
    24601 aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt
    24661 caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt
    24721 ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat
    24781 atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga
    24841 ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac
    24901 ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc
    24961 aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag
    25021 tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg
    25081 aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa
    25141 aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc
    25201 cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg
    25261 aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg
    25321 ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag
    25381 aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa
    25441 tcaaagagtt agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag
    25501 aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt
    25561 ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa
    25621 aaatgtcaga agctggatca cagttaggaa aattcttgaa gaaaacattc caaactgttt
    25681 cagaaacagt gaatgacaac atggaatgga aagatgtcag cttgcgtgtt cctgggattg
    25741 cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata
    25801 tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg
    25861 aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac
    25921 ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc
    25981 gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc
    26041 taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga
    26101 atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg
    26161 gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta
    26221 cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac
    26281 gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg
    26341 tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa
    26401 gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc
    26461 aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga
    26521 caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc
    26581 agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc
    26641 cttattgccg ataggctatc tcttgaacac cgtttaccaa gagattatga acttgccaat
    26701 tatcgccgag tcacaaggaa taatttcaaa aaaagctcgt cacgttttat gaatatggta
    26761 atttgtgacc gagaattttg caaaaagaca ccgattaaga agaaggttgt cacaacgatt
    26821 ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc
    26881 caataaaaat gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga
    26941 tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat
    27001 cattttatac attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta
    27061 tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac
    27121 aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg
    27181 tactttcaac gtattttagt taatacgtta acattcatat cattggctgt tctttttcct
    27241 gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg
    27301 ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca
    27361 ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg
    27421 ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg
    27481 tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg
    27541 aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat
    27601 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
    27661 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
    27721 agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat
    27781 cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta attattattc
    27841 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
    27901 agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat
    27961 ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc
    28021 tgtactgcgt tctcccatct caacttctcg cttattagga gaattatcca gttatttaga
    28081 tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg
    28141 tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa
    28201 acgcggtcat cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac
    28261 ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat
    28321 cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt
    28381 ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga
    28441 cacgaccgtt gaaatcgcgg aagtaggcat tccacaaata aaaatccctg tcaaaacagg
    28501 acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg
    28561 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
    28621 caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
    28681 gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgccatg
    28741 tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt
    28801 atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat
    28861 cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct
    28921 atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt
    28981 atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc acaagcaatc
    29041 ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc
    29101 ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat
    29161 cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg
    29221 tttttacgaa agaaaccaca ccttttgaaa aaaggtgaag tccttttatg ttatctaatg
    29281 tggtatagtt tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta
    29341 ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta
    29401 tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat
    29461 caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
    29521 gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct
    29581 cgccgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
    29641 caacacccaa cacacgaatc aacgttattt accagatcat tgtctacccg attccatcca
    29701 agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc
    29761 aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt
    29821 gatcatccat gcaagtaaag gattagaaca ggggacacat aaaaggatct ctgatatctt
    29881 gttagaggaa atcccagtag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca
    29941 tgcagaagaa gtagcgaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa
    30001 agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc
    30061 tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg
    30121 cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt
    30181 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt
    30241 aagcggagta ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc
    30301 aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt
    30361 cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaataa tggaaatcga
    30421 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
    30481 cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt
    30541 ttgaaggagc gaatgtatat gaaagtcaaa aaagcagtta ttccagcagc aggattagga
    30601 acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa
    30661 ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc
    30721 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa
    30781 acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat
    30841 gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa
    30901 gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa
    30961 gacgaagtac cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg
    31021 atcgccgtta tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga
    31081 gaagtattgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
    31141 aaagcaccaa gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat
    31201 gtattgtccg agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat
    31261 acattgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc
    31321 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta
    31381 ggtgcacctt tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc
    31441 aagatcgatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc
    31501 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
    31561 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
    31621 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
    31681 ttttatcgtt cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt
    31741 agcagaagca aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca
    31801 agtagaaggc ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc
    31861 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa
    31921 tcattctagt aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc
    31981 gacggttgcc ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac
    32041 tgaaacgaca ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta
    32101 gtcggaggca cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa
    32161 cttcgggatg aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca
    32221 gatatggcga aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat
    32281 ctttctcaac aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca
    32341 gatgaagcag gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac
    32401 ttatctaatc gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga
    32461 agtattgcta aagatacttc agaagatatc atgattgatg taaaagacgc tgctggtaaa
    32521 gcaggagata ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa
    32581 gaaatgatga aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca
    32641 atgacagatt catctacaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc
    32701 ggactagctc tcaccttttt tactcttatt tttctaaaac aagtaattct tttggtgtag
    32761 tcgtgaatgg cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta
    32821 ctttttcagg aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta
    32881 agtcatttcc ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta
    32941 aacggtgatt gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat
    33001 caagttcacc tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa
    33061 gagtgatgtt gtagattttt tcttggaagt cggttggttt ttgataagca attgtacgag
    33121 tagcatcact gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg
    33181 gtgaaatctt cgtatttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga
    33241 ctaatgtgtc gaaagacatt tgtgaaacac cttgtttttt caattggtat tcaatttctg
    33301 caatgatcgc ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa
    33361 catcggccca tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt
    33421 gtagacgttg gatgatggaa gttagatcag tgtaagaatc cgttcccgtc aagatatcag
    33481 agatagcatc gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct
    33541 gaccatagcg agcacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat
    33601 agccacggac atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag
    33661 tgaaaaggaa aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat
    33721 cactttcgta tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga
    33781 cttggtgttg tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac
    33841 ctactttcta aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac
    33901 gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt
    33961 cttgagatta tttgatattc tattttagaa atgtaagacg tattttcaga aaacatataa
    34021 aatatgaata ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga
    34081 gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgttaaa
    34141 ccagcaacaa gaaaaaaagt cttggaagtt atcgatcgct tggattatcg tccaaacgca
    34201 gttgcgcgtg gcttggcaag taagaaaaca acaacagttg gtgtaatcat ccctgatgtc
    34261 agcaatatgt tctttgcttc tttagcacgt ggtatcgatg atgtggcaac aatgtacaaa
    34321 tacaatatta tcttagcaaa ttcagatggc aacgaccaaa aagaagtcaa tgtattgaat
    34381 aacttactgg ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat cacagacgaa
    34441 atccgtggag aattttcacg ttcaaaaact cctgttgtat tagctggctc tattgatcca
    34501 gatgaacaag taggtagcgt aaacattgat tatgtatccg caacaaaaga tgcagtaaac
    34561 aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt gatcgatccc
    34621 atcaatggac aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa taacctacca
    34681 tatagcgaag gattgatctt tgaagcacaa tacaacttca aagatggtct atctctagtc
    34741 gatcggatcc ataatagcgg tgcaacagct gtctatgttt cagatgatga attagccatc
    34801 ggtattttgg atggtttatt agaccgtggc gtgaaagtac cggaagaatt cgaaatcatc
    34861 acaagtaaca attcactact gacagaagta gctcgtcctc gtttaacaag tatcacacaa
    34921 ccattatacg atttaggggc tgtatctatg cgcctattga cgaaaatgat gaataaagaa
    34981 gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atctacaaaa
    35041 taataaaagg acaaccattc agaggttgtg tgcctgccgt ttaactctgc gttacaagga
    35101 gcaatttcaa aaaacagcat ctcatgtttg atgaatattg tgactcgtga ttcggagttg
    35161 gcaggagaac accgtttatt tgggaaaagg ggttatgaca aaattttgtt ataatccttt
    35221 ttttgtgtct taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat
    35281 ccgtgtatag atgctgcatt ttgttcacca ccatcaaaaa aataagggac tgtgacaata
    35341 ctattgtcac aatcccttat tactgtgtat tctattaaat caatcatctt tctcatctga
    35401 ttctttagtt gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata
    35461 gttttcatcg gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc
    35521 ccatagcgat tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt
    35581 tccaggtgtt tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc
    35641 gaatttctca tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt
    35701 ggcaagataa ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg
    35761 ccctgcccag ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta
    35821 ttcattggta gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct
    35881 tgagatggca tctttgaatg gtgtagtgat ttttgcattg atcacactac gcatcatgtc
    35941 attcatgatc gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata
    36001 aattacattt cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc
    36061 gccgtttgct aaagcctgaa atccgttggt ctgttgaagc gtcgtgacat ctactgtacc
    36121 taaaggagca gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta
    36181 gtgctcgtaa gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata
    36241 agccgggata ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc
    36301 ttcgttagta gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc
    36361 ataatctgat acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac
    36421 aagaactggt ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg
    36481 attctgactg taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat
    36541 caatacattt ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt
    36601 ttcaacgcca ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata
    36661 tcctttattt tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc
    36721 agacatatca gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt
    36781 catcacagta taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa
    36841 gtcttttgta aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc
    36901 acgatacata ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt
    36961 tagcgcgcct gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat
    37021 ataagcagct tgcggcagcg tcacatcttt tgcatctaca ccaaagattc cttttgcagc
    37081 ttcctggact ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa
    37141 atacgtagca atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat
    37201 ctcgtttgct ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg
    37261 ctgtgtcaaa gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg
    37321 taaaacagct ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat
    37381 aattgcagtt ttcaataaag gtgaaatctt atcagatgaa atagtcgtgc gcatcagatc
    37441 tgtctggatt tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat
    37501 gtttcctagt tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata
    37561 ggcaaaatag ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt
    37621 aataaaaaca gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc
    37681 tttttttctt ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac
    37741 taatggtttt atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt
    37801 tttggcagga atttcttttt taaaagagtt aaaacgtgag aaaatgatct ttattataaa
    37861 agagttctta agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa
    37921 aaatcaatca ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt
    37981 gcctttcaag gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga
    38041 aaatgctgtt aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct
    38101 atacaggcgc ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg
    38161 gaaaagcgta catggcagaa aaaggtctaa caaatatcgt cgttcatgcc ccttatatca
    38221 ttaatttggg gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag
    38281 aagagattgt tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc
    38341 atgtaggggc aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt
    38401 tgacaaaaga acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa
    38461 tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat
    38521 tatccgttac ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact
    38581 tttcaggtgt aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc
    38641 atatcaatga ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt
    38701 ttggaacaat cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata
    38761 tttcgaaaat cttggaaact ccctatgtag gcaaagataa aaagaaagct tacgcaccat
    38821 atggaaaaga aatcgccatg ttcaaagcgc aaacattcga tccagaagtg tttcctgagt
    38881 tattagcaga agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag
    38941 gcacattgcc ttgtcctttt ttgtttaaga aacaaaggag tactttttgc agtctttttc
    39001 atcatgcatc ttctcccccc tctttttaaa tttagacaaa caaggtatac tggtagaaaa
    39061 gtaaaaaaac gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat
    39121 gctgaacact ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct
    39181 tggcatagcc ctattattta caattattcg tttgtattta tatagtaaag caatctatgg
    39241 aacagacatg tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt
    39301 ggacggtaaa tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag
    39361 cttattttta gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt
    39421 cagtattttt gccagtatat tggttgcact cgcgtttaaa ccgattgtga agaacaagta
    39481 tcttctttta agtatctatt tttatttttt gttttctccg attagtttta cacatgaata
    39541 ttccactcga acatatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg
    39601 tttattagct atttattacc gcaaatacgg ttctagcaga aaattgctgc catggttttt
    39661 agggctttct gtcgcttttc ctttcttttg gttcattcgc gaagattcta tctggcttct
    39721 tccttttact gtagtagctt tgggtatcag tttcgtccag atcttgctag ggcaagactt
    39781 ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt cttctctcga aacgggaatg
    39841 gctgaaactt tcattgtttt tacttcctat agttggcttt tccatcgtct atcaatggat
    39901 cgaatataaa aatgagacaa catacggtat tgcaaccatc aatgaccgtt ccagtggaga
    39961 atttggacgt ttgatgaaac aactgatccg aatagatgat gaaacgaact tgaataaaga
    40021 aaacagtgag atatgggtct cacatgctgc actaaataaa gcattagccg catctcctac
    40081 attccatact gtttctaccc gtattgacga attatatacg actcacgcgt ggactcaagg
    40141 tggaaaagta aaagaattac ccggagatat catcttttgg gcattgcgag atgtggtaag
    40201 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    40261 ccaagaatta gaagaagcct atcacaaggg aacaatcaaa aagaaaaaag agttctatct
    40321 gatgggatca ggagatggaa agacgatgag tgatctgcca atcgtattcg atttccttca
    40381 atcaggatac gcttatgcat ggcgatataa agatttccat caaggaggaa attatagttt
    40441 cggaacgcaa gaacaagtca aacaggctca agaattatta aaccttccgc taatggagaa
    40501 ttggatcgat agtaatgaac cgagaacacc taatttccaa ttaacaaaat cagcaaaagt
    40561 cgcgaatgtc atcataaaaa tttaccaagt tctggctatt cccgtgctga ttgcttcaat
    40621 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    40681 tagttcctct ttgattgtgg tcaccggatt aatactgaca caattggttt ttcttttcgg
    40741 tgtctcatgg ttttgttctt atgcgccaga aaaaaaggac tattttttaa gtgtctacac
    40801 tggtgcaggc gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa
    40861 attaaatctg aaaaaagctt tatcttctaa ataaaaagac ccttggtagc tagattttta
    40921 acctaactat caagggtctt tgtgtggaaa aatcagtttt tattcgattt cttctaccgt
    40981 ttcctgcaaa agctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc
    41041 caacaggaga aggacaagaa caatgattac ataagcgatg tgatcagcca atgctagttg
    41101 agcggaagtg gcttgctcgc cgaagaaact ttcgatctgt cctggtaatc ctcgtaagtt
    41161 caagtaagtt agactaatca cagagaacca gccaagaagt tttacgatga atgaattttt
    41221 aaatcggcta cccatctccg cttggctgtt ggtcatcatc agtaaaggaa tcatcgaaaa
    41281 cggcaacgca aaagccaaga acacctggga ttcattcatt aagttattca aagcggcatg
    41341 ttcttcgatc gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga
    41401 aagcaatcgt gtgaccagtc ttcttagcca aatcgggata cgcatatgga tgaatccttc
    41461 catgatgact tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag
    41521 tgctacagca aataaagtgg acaagatacc agattttgct acagcaatca aaataccatt
    41581 gctcatagct gatggatcag ataaagcttc atacagacca aaaaaggaag gatcttggac
    41641 agaaccggtt ttgaagacag cgacacccat gatcaatagc agtgcattga ctagaaaagc
    41701 cattgacaac tggatattag aatcccaagt agaaaaacgt acagcttgtg ctacatcttc
    41761 ttcgttttgg tgatcgatct tccgagtttg tgaaactgct gaatgaagat ataagttatg
    41821 cggcatgact gttgcaccaa taatccctaa agctccagtc agcggagttt cgccgccgat
    41881 gaccggtgag tctgagaatg tatgactgtt agggataaag ccagcaaata aactgtgcca
    41941 atctgggtta gacaagccaa cttgatagac aaaaacgaat aaaatgacga aaattaagca
    42001 gacaaccagt gcttcgatct tacgaaaccc gacttttgtc agtagtaaga gaacgaaaac
    42061 atctaataca gtaataaaga cggcaatcac caatggaata tcaaaaagta agtagagggc
    42121 gattgctgca ccaatgactt ctgcaatatc tgttgccata atggctaatt cagtcaagat
    42181 ccataaaatg acacctaatg tcttacttgt gcgtgctcgg attgcctgtg ccaaatccat
    42241 ttggctcacg attcctaatt ttgctgccat atactgtaaa agcatagcaa tcagactaga
    42301 catcaaaata actgacatca ataaatattg gaaattttgt cctccggtga tcgaagtaga
    42361 ccagttaccc gggtccatat acccaactgc tactaacgca ccaggtccag agtaagcaag
    42421 aagtgtttta aaaaaacttt tcccctttgg tacatcaact gttccattta cttcagctaa
    42481 agataaacca tttgtatgtt caataagtcg atgcttatga aattgtttat tggattgatt
    42541 attcaagaaa aaaccacctt tcctatttat ctacgattac attctacacc tcaaaaagaa
    42601 aaaattaaag aaaaagattc ggtacgccta attttttttc atggcgcgtc ttgctagatg
    42661 acgaaagaaa ttttgttctc ctctaaaaaa accactattt tttgcatgaa actaaagaat
    42721 acgcctttct tttttcaata aactttagta aaataagtca ttgagagcaa ttcggaaata
    42781 gaaaggagag atttatatgt tatcaacagg tattgtcgta cttattgcta ttattgcttt
    42841 gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga
    42901 aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa
    42961 accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata
    43021 ggttctgacc caacgacttt ttacgggctg attttttcag tccgtttttt atttcgtaat
    43081 ttgttagact attcagatta ttcagcaaaa aagacaagca cagacaatcc taggctcttg
    43141 ataaagacaa aggaggagta aaatgtcgat ttttagaaaa ttaggctggt tttttcgtca
    43201 ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt
    43261 agttcctcca aaagtgattg ggatcatcat tgatgagatc gccgataaaa atttatcaat
    43321 aaagatcatt ttcgggtgga tcttcctatt gattatcgct gcttttttac aatatctttt
    43381 tcgttatatc tggcggacga atatttgggg aagtgcagct cgattggaga aagaacttcg
    43441 gcagcagtta ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa aatatcggac
    43501 tggggatttg atggctcacg caacgaatga tttgaatgcg atccagaatg tagcaggagc
    43561 tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat
    43621 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt
    43681 cacttcacga gtgctggggt caaaactcca tgatgctttt cgtgattcgc aagaagcttt
    43741 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    43801 tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa
    43861 aaacaaacga gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg
    43921 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    43981 gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    44041 gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga
    44101 acttttgcat gaaaagacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa
    44161 aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt
    44221 agaacaaatc aagtttgcga tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    44281 tgcgggaaaa acgacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    44341 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    44401 cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt
    44461 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    44521 caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc
    44581 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    44641 attattaata ttagatgatg cactctctgc agtagatgcc aaaacagaag aagctatctt
    44701 gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag
    44761 tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    44821 aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca
    44881 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    44941 acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc
    45001 aaacctttta gaagaacgtt catcgttgct atcttatttg cgtttgcttt gtctgtgatc
    45061 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg
    45121 gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt catagtaaaa
    45181 agtatcattt ggtttttcca atggtatttg tattccatgg cctcattgaa gacctatcag
    45241 tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag
    45301 acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    45361 tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc attccttgcc
    45421 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgtttttgcc gattttagga
    45481 atcgtcatct ggtattacca gaaattcagt tctcgtattt accgaaatat gcgggaaaaa
    45541 ttaagtcagc tgaacacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    45601 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac
    45661 acgcgttatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta
    45721 tatgcattgg cgattgcgtt ggtgttgact ttgtttggga tcgatgcgtt gcattctccc
    45781 gtagaagttg gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg
    45841 tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc
    45901 ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg
    45961 attacttcag gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    46021 gtattaaaca atatctcttt tgttgctaat ccgggagaga ccgttgcatt agtgggacac
    46081 accggaagcg gaaaaagctc gatcatcaac gtattgatgc ggttttacga attttatgaa
    46141 ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    46201 aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt
    46261 cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa
    46321 gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt
    46381 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat
    46441 cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg
    46501 atccaagaag gattggcaaa aatgcgtcaa ggacggacaa cgattgcgat tgcccatcgg
    46561 ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa
    46621 cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    46681 caaagcagtg gtatataaaa gatgttgtga cagtagaatt ttgacctaag cactagagtg
    46741 aaaataaaac atagcttctc atatttttca tctgtgagag ttagtgtcgt agtcaccaga
    46801 tttaaaaaaa gattcgacag ctagtcagaa gtgcctaata gccactaaga aagcgttgac
    46861 aaaagcattt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    46921 tgcaagctaa acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt
    46981 ttgttttgga tacagcccta tacatcatgg aaaggagcaa aacatgctga ttgagaaagt
    47041 actgaataac aacgtcgtaa taaccaggga tgaaagaaat caggaaatga tcgtgatggg
    47101 aaaaggtttg gcattcaaaa agaaagtggg tgacccgatt tttgactcat gcatcgacaa
    47161 aaaatttcag ttgacaaatg acgggctttc acaacagttc caagaattgg cacaagcgat
    47221 tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctaca
    47281 agtccagttg aatgattctg tgtatattgc attgacggat catttgtata cggctatcga
    47341 acgtcacaaa atgggaatat ttgtaccaaa tgtattgctg tatgatatca aacaactatt
    47401 tcctaaagag tatgccatcg ggaaaaaaac agtgaagaaa atctcggata aataccacat
    47461 caagttatca gaagatgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat
    47521 tcaaagccag caagacaata cctacctgtt tactataaca atacaggata ttctaaatat
    47581 cgtccgctat tactataaga ttacatttga tgaagattca gtccattttt ttcgattcgt
    47641 cacacactta cgtttttttc tgacacgcgt cctagatgga gaaaaatcag aagtgggaga
    47701 agtttctgag gaattgtttc aggttattca aaaaaaatat aaggatacgt ttggttgcgt
    47761 tgagaaaata gcagactttt tagccgagac gttcaactac cagatgtcca cggacgaaaa
    47821 aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaaaaaa
    47881 ggaggataaa ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct
    47941 tccactaata aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc
    48001 gtagaaaatg tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt
    48061 cgtttcaaat taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt
    48121 attgtaacgg taatgcgagc gggagggcag tatcaagttg tcattgggaa tcatgtgcca
    48181 gtcgtgtttg aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca
    48241 gaaaaaatga gaccgtttga tatgcttatt gatggaatat cgggctgttt ccagccattc
    48301 cttgctatcc tagctgctgg cgggatgatt cgtggactga cagcattcct tgtcttctta
    48361 ggcgcgtttg accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta
    48421 ttccaattta tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa
    48481 ttcgtaggga tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg
    48541 agtggtgcag cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct
    48601 atttatcaaa cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt
    48661 cccattatct ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta
    48721 cctgagatga tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta
    48781 ggttttttac tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt
    48841 gaaacacttt tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa
    48901 attctggtga tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct
    48961 gtgaatggtt ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca
    49021 gcacttaccg cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca
    49081 gccattgttt ctggtattgt ggggattaca gagccagcta tttatgggtt tacattacca
    49141 cgtaaaaaaa tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg
    49201 ttaatgaatt taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat
    49261 attagaccag atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg
    49321 atggtggtat cctttacgct gacttttttc ttttggaagg atgaagcagg tgaaacagat
    49381 gatatacaga aaaaatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct
    49441 ccactaaatg cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg
    49501 atctaccctg aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gaccctgttc
    49561 ccgacgaaac aggcaattgg aatggtttct gaaacagggc tggaattgct gattcatgtc
    49621 ggattggata ctgtgcagtt ggaagggaaa tattttgaaa gcttagtgca gcaaggataa
    49681 agagtgaaac aaggcgatct gttgctacgc ttcgacaaaa agaaaatcga aaaggaagga
    49741 tacagtttag aaacacctgt gatcgtcacc aattacacgg attatttgga tattttggaa
    49801 aaccaagggg agacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt
    49861 aggtgaagaa tggactaggc ttaactattc tttttattcc acttctttat tttcaaaatt
    49921 gaaaggctct ttcatgatgg ctgagctgat agggcgttta tcgaattttt gaccgacaga
    49981 aattagatgg actaaattct gttgataatt ttctgcaaaa acacaactat ataacacgtc
    50041 tagaagataa caaattgatg tattgatcgt aaaattacca atttttgaat acagtcgctc
    50101 gcgtgtcgta agtcgcaaag cggctgtact cattgcagat agcgttgaat caccgatact
    50161 ggtcaaagct aaaattggaa catgattttg tttcaatatt tctgcggtac gaaggatcac
    50221 attgttttca cccgtataag aaatcagaat cccgcacata tcactcgtgc aattgtacgc
    50281 ttcatagaat gattcaccaa aagtggtgac aatcgtgaca tccattttga tgcgcttcat
    50341 tttcagcatg aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt
    50401 ttttgcttga agcattagtt gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag
    50461 tgtatcatga atcgtcatct gttctaaaga agccatttta tttgcgatcg tcataatacc
    50521 gtctgtggtg gaaaaaggaa gattcgcatc tacgtcttga aaattccctt caagataggc
    50581 tgtttcagct aaaaaagcat tacgaaaact gacccagcca tcaaaaccca ttttttttgc
    50641 tacacggatg agtgtagagg ggtggacata acatcgctca gcaatcgctt ggatggtcat
    50701 ttcgccaagt gctggagaat cgcttaagag aaactggatc acttcatttt ctgaaggaga
    50761 aaaggagatt tctttgattt tttcacgtaa taacatgtag ataccttctt cttatttttt
    50821 tacgtaaatt ataacttttt tgaaaatgat ttgtaaacgg atttatacta tataacttaa
    50881 aatagacatt ttttgacaag atatttacaa aaatccacta gaaaacagta aattttgtaa
    50941 atgttactgg aaagaaacag atttctattg tttgttttgt ttacaaaaaa gataatgaaa
    51001 gtgtaaaaaa gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg
    51061 gaggcagcag ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac
    51121 cgatacgaga aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtta
    51181 gtcagatgtc tcagcgtatg tgggatgctt ctccgtatga agtcaagatc tatgcaactc
    51241 tggatcggga agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc
    51301 aattagatgc acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag
    51361 aaacaaatgg tgctggcggg atattcaaag cttttcgtac cgttccagtc gttttggcaa
    51421 tcgttgagga catgaagcgt ttatgtccaa atgcttggct gatcaatttc gcgaacccaa
    51481 gtgggatggt gacagaagca gttatcagat atggcaaatg ggataaagtg attggattat
    51541 gcaatgtgcc agtgatggcg atgatgacag aaccagcaat gcttggcaag aaaaaagaag
    51601 agctgatcta caagtttgcc ggtctaaatc atttccattg gcacaaagta gcagataata
    51661 aaggaaatga ccgcacggca gaattgattg atcgtttata cgatgaagag aatggattac
    51721 cgaaaaatat ctttgatatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc
    51781 cttgtggcta ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag
    51841 aatatcaaac gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctatttg
    51901 aattatacaa agatccagaa ttagaccata aacctgaaca attgcaaaaa cgaggtggcg
    51961 cttattattc agacgctgcc tgtgaaacga ttgcttcaat ctatggaaat aaagaaacac
    52021 agatcgtggt atctacaaag aatgatggag ctgttcctga cctgccggca gattgtgtag
    52081 tagaagtcac tgcctatgtt ggcgcacaag gagcgagaaa tgtagcgttt ggttctcttc
    52141 ctgctgctga aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag
    52201 cagctgtgac tggtgattat gggacagctt tacaggcgtt tacgatcaat ccacttatac
    52261 gatcaggaaa gacagcaaaa cggatcatgg atgagttgtt tattgcacac cagcagtatt
    52321 taccgcaatt caaggagaca atcagccgat taaaagaaga aggaatcgaa gttcaagatg
    52381 aactggcaag agaattatca ggtaatacac atataatgga ggaagtataa tgggattcag
    52441 aaaagatttt ttatggggcg gtgctacagc agccaatcaa tgtgagggcg gttatgatga
    52501 aggtggacgc ggactagcaa acgtagatgt tgtgccaatc ggaaaagacc gttttcctgt
    52561 tatcaccgga aagaaaaaaa tgtttgaatt tgatgaggat catttttatc cagcaaaaat
    52621 tgcaatcgat atgtatcatc gttataaaga agatattgct ttgtttgcag aaatggggtt
    52681 caagacttat cgaatgtcca tcgcctggag tcgtattttt ccaatggggg atgagaaaga
    52741 gccgaatgaa gctggcttga aattttatga ggatgccttt aaagaatgcc aaaagcacgg
    52801 aattgagcca cttgtgacga ttacgcattt tgattgtcca atgcatttga tcaaagaata
    52861 tggtgcgtgg cgttccagag aactcgttgg tttttatgaa aatctgtgtc gagtcatctt
    52921 caatcgctat aaaggacttg tcaaatattg gctgacattc aatgagatca atatgatcct
    52981 tcatgcacca ttcatgggcg cagggctgta ttttgaaaaa ggagaaaatg aagaacaaat
    53041 caaatatcaa gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agattgctca
    53101 tgaagtagat ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata
    53161 cacttgcaat cctgaagatg tttgggcttc tagaaaatca gatcgagaaa attatttctt
    53221 cattgatgtc cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggagcgaaa
    53281 tggcattcag atcgaaatgg aaccagggga tgccgcattg ctgaaagaac atactgttga
    53341 ttttatctct ttttcttatt atgcttcccg agtttcgact accgacaacg aactcttgga
    53401 acagactgca ggaaatatct ttgcttctgt caaaaatcca tatctgcaag caagtgaatg
    53461 ggggtggcaa attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta
    53521 tcaaaaacca ttgtttattg tagaaaacgg cttaggtgca gtcgatacgc cagatgaaaa
    53581 tggttatgtg gaagatgatt accgaatcga ttacctggca gcgcatatcc aggcgatgaa
    53641 ggatgcagta gaactagacg gagtcgatct attaggctat acgacttggg gatgtattga
    53701 tttagtatca gctggtaccg gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg
    53761 tgataaccaa ggaaatggca cattagcccg ttcaaagaaa aaatcattca actggtataa
    53821 aaaagtaatt gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag
    53881 tctattgttt ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga
    53941 acaatagact ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa
    54001 tctacatatg tatgatgtaa tcgttattgg cgcaggtcct gctgggatga ctgctgcttt
    54061 atatgcttca cgttcaaact tatccgtatt gatgatcgaa caaggtgccc ctggcggcca
    54121 aatgaacaat acggcagaaa tcgaaaatta tcctggattc gattcgatca tgggccccga
    54181 tctagcttat aagatgtatg aaagtgtctc aagatttggg acagaaaatg cttatggaat
    54241 cgttcaagat atcaaggatc acggcagcca taaagaagtc atctgtgaag ataaaagtta
    54301 cgaaggaaaa acagtcatta tcgcaacagg atgtatccac cgtaaattag gcgtacctgg
    54361 cgaggaagaa tttgctggac gaggcgtttc ttactgtgca gtctgtgatg gtgcgttttt
    54421 ccgtaacaaa cgtctagtag tcatcggggg tggggactca gcagtagaag aagccatcta
    54481 tttgacacaa tttgcttctg aagtagtgat cgtccaccgt agagatgaat tacgtgcaca
    54541 aaagatcatc caagaccgtg catttgcaaa tgaaaaaatt tcctttatgt gggatacagt
    54601 cgttgacgaa atcgttggaa acgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa
    54661 aaccggcaat gaatttgatt tagcagcaga cggagtattc atctatgttg gactggatcc
    54721 attaactgca ccttttagag aagcaggttt gacgaacgaa gctggctgga ttccaacaga
    54781 caacgaaatg aaaacgaata tgccaggcgt ttttgccatc ggagatgttc gtgaaaaaga
    54841 tttgcgacaa atcactacag ctgttggtga cggcggtatt gctggacaac aagtgtataa
    54901 atacttggag tctttagaag aaacaacagc ataagtttaa aaagagtaga agaacgggtt
    54961 caattgttct tttactcttt tttttcgcgt gaaatttact gaatctgatt tatgcgaaca
    55021 cgtccaacac cttttgataa aagaatcctt tcttgattta agcgtttttt ggtcctcata
    55081 ctttcatatc taataagtga atgctatact tattaagaac catatttcag aaagaggtgt
    55141 tattttcatg tcttgggaac aagtgtatga acaatgggta catgaagaaa atttagaaga
    55201 aaacttaaaa aaacaattaa atgatctggg tgaagatcct gaaaaacttg aagatgcttt
    55261 ttatgcaccg ttggaattcg gtactgcagg tatgcgggga atcttaggac ctgggatcaa
    55321 tcgaatgaac atctatacgg taagacaagc aacagaagga ctagcacgtt tcatggatac
    55381 acaaggtgaa gaaacaaaac gccgaggtgt cgctatcgct tatgactcac gccatcagtc
    55441 accagaattt gcaatggaag cagccaaaac actagcacat cacaatatcc cgtcttatgt
    55501 attcgaaagt ctgcgtccaa caccagagct ttcttttgca gttcgtcatt tgaagacgtt
    55561 tgcgggaatc atggtaaccg catctcacaa cccagcggct tacaatggat ataaagtcta
    55621 cggagaagat ggcggacaaa tgcctcctgc agacgcagat gcattgacga aatatgtccg
    55681 tgaagtcagc aatccgctaa aagtcgaagt attatctgac gaagaagcta aacatagcgg
    55741 gttgatcacg atcatcggag aagaagtgga cgctgcttat ctagaagaaa tcaaagcagt
    55801 gacgattgat cgtgaattgg ttgaaacaat ggggaaagat ttgaaacttg tgtacacgcc
    55861 acttcatgga acagggaaaa tgttgggcga acgtgcgttg aaacaagctg gattcgaaca
    55921 attcgtacta gttcccgaac aagctgtggc agatccagat ttcactacgg tcaaatcgcc
    55981 aaatccagaa gaacattcag cttttgaata tgctatacga cttggagaaa aagaaggagc
    56041 tgacttgctg attgcaacag atcctgatgc agaccgttta ggcgcagcgg ttcgtttgcc
    56101 agatggttct tatgaagtat tgactgggaa ccaaatcgga gcattgtttg ctcaatatat
    56161 tttggaagca catcaatcaa gaggcacttt accagaaaac gcagcggtat tgaaatcaat
    56221 cgtttccagc gaattgccta cagcaattgc ggaacactac ggtgctacca tgttcaatgt
    56281 gttgacaggg tttaaattca ttgctgaaaa aatccaacaa tttgaagaag accattcaca
    56341 tacttttatg tttggattcg aagaaagtta tggttattta gttaaacctt ttgttcgcga
    56401 taaagacgcg atccaagcat tgcttttatt agcagaagta gcagcttact ataaaaaaca
    56461 aggcaaaacg ttgtatgacg gcttgcaaac gatttttgaa acctatggct atttcgcaga
    56521 aaaaacgatt tctgtgacaa tgagcggaca agaaggttct gcgaaaatct ctgctttgat
    56581 ggaaaaattc cgtaaagaag caccgacaga atttgccgga gcaaaagtta ttcaaacaga
    56641 agactttaaa gaattgacga gaacagaccg tgaaggaaac gtagaaaaac taacaacacc
    56701 gccatccgac gtattgaagt atacattgga agaaaatggc tggattgcta tccgcccttc
    56761 aggaactgag ccgaaaatca agttttatat tggtgtaaaa ggaagttcta cagaagacgc
    56821 agccgaaaaa gtagcctctt atgaagcgat tatcaaaaag atgacagaag aataaaaaac
    56881 ttgagactga tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg
    56941 aatggattgc cagacacgca aaaatcaaaa gtagagaaag tcagtcaatt attcaaaatg
    57001 ttgagtgatc ccactcgtct taaaattctg ctatatctga aagacgggga acaaaatgtg
    57061 acagcaatca cccaagcagt ggaaatggag cagtctgctg tatcgcatca actacgttta
    57121 ttaagagaaa atcatgtcgt caagtctcat cgcgagggaa aagcgatttt gtatagtctt
    57181 gatgattctc atgtactgga tattttgaac cagacactca aacatgtgga acatcaataa
    57241 cgataggtgc aaatagataa acactggagt gtgacaacga agaaatttcg ttgtcacact
    57301 ccagtgtttt ttaagaaaaa gacaaaaatg atcaatcgct tttttctgtt gtttcagttc
    57361 cataataact atgatcttct aggtctaaag cattcaaaat aagcgaagcc gtttcttcaa
    57421 tagataaaga tgctacattg atcactacac atcctagttt ttcataaaga tcattcgcaa
    57481 aatcgagttc agctctaatt ttttcgatat cggaataagc agtatcaggg tttaatccgt
    57541 aagagcgcat ccgttcttta cggatattat ttaaaatatc tgggttattt gttagtccca
    57601 cgattttttt aggatcgatc tcccaaagct gtttgggaat atgtgcttgt gggatcaaag
    57661 gtaaattggc aacttttaaa tttttgttag ctaaaaacaa gctcaaaggc gttttggaag
    57721 ttcgggatac gcctagcaaa acgacatctg cttctaaaaa accacgagga tctttcccat
    57781 catcgtattt tacagcaaat tccattgctt tgatccgttt gaaatagttc tcattcaata
    57841 aatggagggc accaggttgc cgaattggct tgataccagt taaacgttca atttctgata
    57901 tcggtggcgt catcacatca aaactaaata attgatgttc ttcacaaaaa gaacgtgtta
    57961 tctcgatcag ttcttgattg attaatgtat gcaggacaag tgctttttct tctaatgcat
    58021 cttgcaatgc ctgccttaat gttttttcat ctgtcacgaa cgttctgcgg aataggttga
    58081 actcaacggt aggatactga gccatagtag cttgcgctag ttttgtcgca gtttctccag
    58141 cagagtcgga gatgacaaaa aaagtaacga tttctgtaga atcggaagtc atggataaaa
    58201 cctcacttgc atttttttta tgtttattat accatactct agaatgcgta cttcgtaatc
    58261 attttgattt ggagggataa gtcatgaaaa aacagacatt gatcgaacaa tcattagaaa
    58321 cattgaaaaa taacggtttt aaatatacta aaaaaagaga agcattgtta acttatttag
    58381 tgaagcgtaa ccgctacgta tctgctaaag aagtttttga ctatatgaat caggaattca
    58441 aaggggtaag ttatgatacg atttaccgga atctccatga ttttgcagag atcgatttac
    58501 tggaagaaac cgatttaaat ggagaaatga agtttcgttt tcattgctgt caggggaaaa
    58561 tcggccatca ccatcatttt atctgtacag tgtgcggaac aacgaaagaa atccatatgt
    58621 gtccgatgaa ttatttcgaa gaacagctag gtggatgcac gatcgaaggg catagattcg
    58681 agattttcgg aagatgtgaa aattgtcaat aaggatttaa ataaaataac atttgataat
    58741 tttaaaaaaa agcaagattt ccttgacggt caaaacagca ttagatataa tatttacagg
    58801 acagtcttgt tttttgagat atatttcaat aacaattacg gttacttttt aagctcggag
    58861 ggagggaatt aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct
    58921 tcgtcgcttc aaacgttccg tgtcaaaagc gggtacttta caagaatctc gtaaacgtga
    58981 attctacgaa aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa
    59041 aaaattctag ttctgtgatg aattggagtt ggtagtgtgt cactactaag tagattaaac
    59101 gatgacatga aagcagcgat gaaagcgaaa gataaggaat ctttacaagt catccgaatg
    59161 atcaagtcat ctatacaaaa cgagcagatt aaagaaggtc acgacttgac agaagaagaa
    59221 gaactaacgg tgttgtctcg tgaaatgaaa cagcgacgtg attctttgca tgagtttgaa
    59281 gaagcaggac gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat agtcgaaaaa
    59341 tatatgccgg aacaactttc cgacgaagaa attcgacaac ttgttcaaga agcaatcact
    59401 caaactggtg cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat tatgcctaaa
    59461 gtcaaaggaa aagcagatgg aaaccaagtg aatgcgattg tgaaagaact attacaatct
    59521 taattttatc atttaaactg cggacagtga ttatccgcag ttttttgtgt tcgtaaaaaa
    59581 agaactaaaa aacagctaga agaaagaatc ttattcttt
//
LOCUS       DACDHL010000009        58383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDHL010000009 DACDHL010000000
VERSION     DACDHL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.009"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..832)
                     /locus_tag="IV228_000574"
     CDS             complement(<1..832)
                     /locus_tag="IV228_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8716513.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     Y"
     gene            complement(1056..2996)
                     /gene="thrS"
                     /locus_tag="IV228_000575"
     CDS             complement(1056..2996)
                     /gene="thrS"
                     /locus_tag="IV228_000575"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8716514.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3333..3512)
                     /locus_tag="IV228_000576"
     CDS             complement(3333..3512)
                     /locus_tag="IV228_000576"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8716515.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3559..5382)
                     /gene="cadA"
                     /locus_tag="IV228_000577"
     CDS             complement(3559..5382)
                     /gene="cadA"
                     /locus_tag="IV228_000577"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8716516.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5660..7939
                     /locus_tag="IV228_000578"
     CDS             5660..7939
                     /locus_tag="IV228_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8716517.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8032..8679)
                     /locus_tag="IV228_000579"
     CDS             complement(8032..8679)
                     /locus_tag="IV228_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716518.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8858..9577)
                     /locus_tag="IV228_000580"
     CDS             complement(8858..9577)
                     /locus_tag="IV228_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8716519.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9708..10859)
                     /locus_tag="IV228_000581"
     CDS             complement(9708..10859)
                     /locus_tag="IV228_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8716520.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11076..11816)
                     /locus_tag="IV228_000582"
     CDS             complement(11076..11816)
                     /locus_tag="IV228_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8716521.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11820..12143)
                     /gene="rsfS"
                     /locus_tag="IV228_000583"
     CDS             complement(11820..12143)
                     /gene="rsfS"
                     /locus_tag="IV228_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8716522.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12200..12793)
                     /locus_tag="IV228_000584"
     CDS             complement(12200..12793)
                     /locus_tag="IV228_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8716523.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12777..13424)
                     /locus_tag="IV228_000585"
     CDS             complement(12777..13424)
                     /locus_tag="IV228_000585"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8716524.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13437..13748)
                     /gene="yhbY"
                     /locus_tag="IV228_000586"
     CDS             complement(13437..13748)
                     /gene="yhbY"
                     /locus_tag="IV228_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8716525.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13764..14873)
                     /gene="yqeH"
                     /locus_tag="IV228_000587"
     CDS             complement(13764..14873)
                     /gene="yqeH"
                     /locus_tag="IV228_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8716526.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14870..15397)
                     /locus_tag="IV228_000588"
     CDS             complement(14870..15397)
                     /locus_tag="IV228_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8716527.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15577..16362)
                     /locus_tag="IV228_000589"
     CDS             complement(15577..16362)
                     /locus_tag="IV228_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8716528.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16355..17224)
                     /locus_tag="IV228_000590"
     CDS             complement(16355..17224)
                     /locus_tag="IV228_000590"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8716529.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17225..18604)
                     /locus_tag="IV228_000591"
     CDS             complement(17225..18604)
                     /locus_tag="IV228_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8716530.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18612..19031)
                     /gene="fabZ"
                     /locus_tag="IV228_000592"
     CDS             complement(18612..19031)
                     /gene="fabZ"
                     /locus_tag="IV228_000592"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8716531.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19056..19532)
                     /gene="accB"
                     /locus_tag="IV228_000593"
     CDS             complement(19056..19532)
                     /gene="accB"
                     /locus_tag="IV228_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8716532.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19535..20770)
                     /gene="fabF"
                     /locus_tag="IV228_000594"
     CDS             complement(19535..20770)
                     /gene="fabF"
                     /locus_tag="IV228_000594"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8716533.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20789..21526)
                     /gene="fabG"
                     /locus_tag="IV228_000595"
     CDS             complement(20789..21526)
                     /gene="fabG"
                     /locus_tag="IV228_000595"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8716534.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21538..22455)
                     /gene="fabD"
                     /locus_tag="IV228_000596"
     CDS             complement(21538..22455)
                     /gene="fabD"
                     /locus_tag="IV228_000596"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8716535.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22562..22786)
                     /locus_tag="IV228_000597"
     CDS             complement(22562..22786)
                     /locus_tag="IV228_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8716536.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22839..23801)
                     /locus_tag="IV228_000598"
     CDS             complement(22839..23801)
                     /locus_tag="IV228_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8716537.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23811..24251)
                     /locus_tag="IV228_000599"
     CDS             complement(23811..24251)
                     /locus_tag="IV228_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8716538.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24557..25522
                     /locus_tag="IV228_000600"
     CDS             24557..25522
                     /locus_tag="IV228_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8716539.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25579..25809)
                     /locus_tag="IV228_000601"
     CDS             complement(25579..25809)
                     /locus_tag="IV228_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8716540.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25895..26782)
                     /locus_tag="IV228_000602"
     CDS             complement(25895..26782)
                     /locus_tag="IV228_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8716541.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26871..28094)
                     /locus_tag="IV228_000603"
     CDS             complement(26871..28094)
                     /locus_tag="IV228_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8716542.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28111..29190)
                     /locus_tag="IV228_000604"
     CDS             complement(28111..29190)
                     /locus_tag="IV228_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8716543.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29321..31048)
                     /gene="ptsP"
                     /locus_tag="IV228_000605"
     CDS             complement(29321..31048)
                     /gene="ptsP"
                     /locus_tag="IV228_000605"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8716544.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31048..31314)
                     /locus_tag="IV228_000606"
     CDS             complement(31048..31314)
                     /locus_tag="IV228_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8716545.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31793..34027
                     /locus_tag="IV228_000607"
     CDS             31793..34027
                     /locus_tag="IV228_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8716546.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34175..34495
                     /locus_tag="IV228_000608"
     CDS             34175..34495
                     /locus_tag="IV228_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8716547.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34700..34924)
                     /locus_tag="IV228_000609"
     CDS             complement(34700..34924)
                     /locus_tag="IV228_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8716548.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35122..36702)
                     /locus_tag="IV228_000610"
     CDS             complement(35122..36702)
                     /locus_tag="IV228_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8716549.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37103..38479)
                     /locus_tag="IV228_000611"
     CDS             complement(37103..38479)
                     /locus_tag="IV228_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8716550.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38607..39725)
                     /locus_tag="IV228_000612"
     CDS             complement(38607..39725)
                     /locus_tag="IV228_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8716551.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39867..40301
                     /locus_tag="IV228_000613"
     CDS             39867..40301
                     /locus_tag="IV228_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716552.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40379..40912)
                     /locus_tag="IV228_000614"
     CDS             complement(40379..40912)
                     /locus_tag="IV228_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8716553.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41006..42184)
                     /gene="mutY"
                     /locus_tag="IV228_000615"
     CDS             complement(41006..42184)
                     /gene="mutY"
                     /locus_tag="IV228_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8716554.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42177..42974)
                     /gene="recX"
                     /locus_tag="IV228_000616"
     CDS             complement(42177..42974)
                     /gene="recX"
                     /locus_tag="IV228_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8716555.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43081..44463
                     /gene="rlmD"
                     /locus_tag="IV228_000617"
     CDS             43081..44463
                     /gene="rlmD"
                     /locus_tag="IV228_000617"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8716556.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44637..45215)
                     /locus_tag="IV228_000618"
     CDS             complement(44637..45215)
                     /locus_tag="IV228_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8716557.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45537..46007
                     /locus_tag="IV228_000619"
     CDS             45537..46007
                     /locus_tag="IV228_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8716558.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46115..47341)
                     /locus_tag="IV228_000620"
     CDS             complement(46115..47341)
                     /locus_tag="IV228_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8716559.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48095..48295)
                     /locus_tag="IV228_000621"
     CDS             complement(48095..48295)
                     /locus_tag="IV228_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8716560.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48429..49037)
                     /locus_tag="IV228_000622"
     CDS             complement(48429..49037)
                     /locus_tag="IV228_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8716561.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49122..49592)
                     /locus_tag="IV228_000623"
     CDS             complement(49122..49592)
                     /locus_tag="IV228_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8716562.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49640..50554)
                     /locus_tag="IV228_000624"
     CDS             complement(49640..50554)
                     /locus_tag="IV228_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8716563.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50700..51503
                     /locus_tag="IV228_000625"
     CDS             50700..51503
                     /locus_tag="IV228_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8716564.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51681..52628
                     /locus_tag="IV228_000626"
     CDS             51681..52628
                     /locus_tag="IV228_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8716565.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52696..52989)
                     /locus_tag="IV228_000627"
     CDS             complement(52696..52989)
                     /locus_tag="IV228_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8716566.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(53017..53682)
                     /locus_tag="IV228_000628"
     CDS             complement(53017..53682)
                     /locus_tag="IV228_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8716567.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53822..54454)
                     /locus_tag="IV228_000629"
     CDS             complement(53822..54454)
                     /locus_tag="IV228_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8716568.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54461..55528)
                     /locus_tag="IV228_000630"
     CDS             complement(54461..55528)
                     /locus_tag="IV228_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8716569.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55525..56256)
                     /locus_tag="IV228_000631"
     CDS             complement(55525..56256)
                     /locus_tag="IV228_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8716570.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56425..56904)
                     /gene="greA"
                     /locus_tag="IV228_000632"
     CDS             complement(56425..56904)
                     /gene="greA"
                     /locus_tag="IV228_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8716571.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57040..58215)
                     /gene="mltG"
                     /locus_tag="IV228_000633"
     CDS             complement(57040..58215)
                     /gene="mltG"
                     /locus_tag="IV228_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8716572.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT        16827 a        12316 c         9867 g        19373 t
ORIGIN      
        1 aatattcttg gattgcttct cgtgtttttt tcactttttc gctaagcttt tcgtttttag
       61 ccatatagaa ataaaaaatg atatcgatcg tagcaaattg ggccaaaagt gaattagttg
      121 ccgcacttcg attcgtgatt tcttttggtc tagatgtttg tacgagtaca tcggctatct
      181 tgctaagcgg gttatttcca aattgcgtca acgcaatgat ttcgatattc atgcttttcg
      241 ccagctcggc taatgcgact acatctgcac ttctacctga gtttgaaaca agccaaagca
      301 ctttcttttt ctcattgctg acaagttgcg ggagcaagac gtggatatct ttttcaaaaa
      361 tgattggctt accgacacga gaccattttt gcagaatatc ttctactact aaagaagagg
      421 cgccaacccc atataaaaaa acagtctctg ccgattccaa acactctacg actttttgga
      481 tcgtttcttc ttctagaata tctactgttt cttttatcgc tacttgtgca ttgttaccaa
      541 gtttgttttt gatagagtaa aaggactcat ttgcttctat atcactaaaa ccttcatcga
      601 ttccagtctg tagttctgtt gaaatcgtga ttttgaagtc cgtcagactt tgaaaaccga
      661 tttttttgga aaaacgtaca actgtcggcg cactcaaccc ggaagcttct gctaattcat
      721 tagctgtcat tgtagggacc ttctcaatat taccgataat gtagttggcc aacttttttt
      781 ctgctgaaga ataatcttcc atcatacttt caatacgact aattacgctc ataagttcct
      841 ccaccctttt aatgatagtc tatgtaagta taccatatca gacccttatt cacggtcgta
      901 tactggataa aaaacaaaaa aacaatccgc ccaacagaat atcttatccc atcaatccca
      961 cagattctat tgagcatctg tgtctttgag gaatacctgt tatctattgt tcggattgct
     1021 ttctatctac tactttttta caggaaattt acctattagt tttctctgct gtaatttttg
     1081 acttctgctt ccatagcatc aataaagata ttcaaatctt ctacagctgt ttctttactt
     1141 ccataacgac gtacgttcac tgttgcatct tccaattcct tgtctccaac aactaattgg
     1201 taagggatct tttgtgtttg agaggcacgt atcttatagc ccattttttc attacgatca
     1261 tcaacttcta cacgcaaccc cttcatttgc aaacgttctt tgatttcata agcataatcg
     1321 ccatgtgcat caacagaaac agggatgatt gttgcttgta taggtgccaa ccatgttgga
     1381 aaagcgcctt tgtatacttc tgttaaataa gcgacaaaac gttccattgt agaaacaatt
     1441 ccgcggtgga taactactgg acgatgcgta ttttccccgt cttctccaac atatgtcaaa
     1501 tcaaagcgct ctggcaataa gaagtctaat tgtatagttg ataatgtttc ttctgtacca
     1561 agagctgttt tgacttgaac atctagtttt ggtccgtaaa aggctgcctc gccttctgct
     1621 tcaaagtaat ctagttctaa ttcatccata gcggctttca gcattgcttg ggctttttcc
     1681 cacattgcgt catcatcaaa atatttatct gtattgtttg gatcgcgata gctcaagcgg
     1741 aaacgataat ctgtaatatt gaagtcagca tatactgcca ccatcaattc aagcgtacgt
     1801 ttaaattcat ctttgatttg atcaggacga acaaatgtat gaccatcatt caacgtcatt
     1861 tctcgtacac gttgtaaacc tgacaaagca cctgattttt catagcgatg catcattcct
     1921 aattcagcga tacgaattgg taattcacga taagaatgga tcgtgttttt gtagaccatc
     1981 atgtgatgag ggcagttcat tgggcgaaga acaagcattt cgccatctcc catatccatt
     2041 ggcgggaaca tgtcttcttg ataatgatcc cagtgtccag atgttttgta caactcaaca
     2101 tctcccatta ttggtgtata tacatgttgg taacctagac taacttcttt gtctacgata
     2161 taacgttcga tcgtgcgtcg gattgttgcc ccttttggaa gccagaatgg caaaccagaa
     2221 cctacttctg gggaaaccat gaacaagtca agttcttttc ctagtttacg atggtctctt
     2281 tcttttgctt cttcacggag acggatatat tctttcaaag ctttcttgtc aaaaaaggct
     2341 gtcccgtata cacgttgcat catttgattg tttgagtttc cacgccaata agcacctgcc
     2401 actgataata atttaaatac ttgtatacgg ccagtggatg gaacatgtgg tccacgacac
     2461 aaatcgatga attcgccttg ttgataagca gtaatcactt cttcttctgg caattcacta
     2521 atcagttcta ctttgtacgg atcacttgca aaaatttcta atgcttcctg ttttgaaaga
     2581 acacggcgtt cgatcggcag attttctttg acgattttca tcatttctgc ttcgattgcc
     2641 ggcaaatctt ctgcagttac ttggttttgt ccattatctg tatcgtagta aaaccctgaa
     2701 tcgatagctg gtccaacgcc aaagtggata tttggataga ggcgacgcat ggcttgggcc
     2761 atcaaatgag ctgctgaatg acggactaag ccaagtgcat cttcatggtc tggtgtgatg
     2821 atctcaatac ttgcatcttc ctcgattgga cgagtcaaat caacgagttt tccgttcact
     2881 ttaccagcta aagctttttt tgctaagctt tttgaaatgc tttcagcaat tgccaatgtt
     2941 gttgttccag actcaaattc tttgactgca ccatctggaa aagtaatttt tatcataata
     3001 gtttcctcct tgaaatgttt tgattgatca gatcatcaag ctgaaaaaca aaaaaatcct
     3061 agcatctctt gaaaagagat actaggacga aaagttcgtg gttccaccta atgttcaaac
     3121 gcaaatgcgt tcctcaagcg ttgtaacggt ccgaccgccc ttgcttcaac aagggtttcg
     3181 aaagtggtca tcctttggtt ttctcctgga aaagtttcag cctagctttt cctctcttat
     3241 cgaagactct caaattcagt tggctttctc atcaatcaat tatgtgttta tttaaccact
     3301 aataaataga aaaatcaagt gggaaactaa acttattttt tccattcccc atttacaccg
     3361 tatgttcctt ttttcatctc attgatgatg acatggatgt gttcttttgg tgcctgcgtg
     3421 tttttatgaa cagcttccgt gatatctttc atcatatttt ctaattgttc cggactgcga
     3481 ccttctacta attcgacatg tacgaatggc ataatgctct ctcctctttc tgatcgttct
     3541 tttttcaact cgattctatc atactatgtt tgtcttgggt actgaatcag gaaaaatggc
     3601 tggatctttt tttgctctca aggcactcaa aatagagacc gtatccacga cttcttgcaa
     3661 aacagctccg aataatgcgg ggattagtcc cgtacttgca attaacatca aaaaaataca
     3721 aataaagatg ccaatcagta cagactgttt cgctattctc atcgtatctc ttgaaatcac
     3781 tactgctttt gctacacgca agagatcatc tttcaagatg accgcatctg cactttcact
     3841 agcagctgta gaaccatgag cccccattgc aataccgata tctgcagcag caagtgctgg
     3901 tgcgtcattc acgccgtctc ctaccatcgc aacaggtctt tgatctttag ggatactttc
     3961 taaaatggtc agtttatctt gaggcaagct acgggcatgt acagaatcta attggacttt
     4021 ttctgcaatc gtttgagcaa tggcagatcc atccccggtg atcatcatca aatttttcac
     4081 atcaagtttt cttagctcct tgattgtctt tgctgcttct gggcgaatct gatccgaaaa
     4141 taggatcgtt cctgcaaaag atcgatcaat tgttacatag attgctgtat tggacgtttc
     4201 tatttcttca gctgaaacaa aagaggcacg tccaatcttt acttcatgat tattgactac
     4261 tgcttggatt ccttgaccgg tcacttcttt cagttgacta acaggataca gttggctgtt
     4321 tttcacgttg ttcactaacg aacgagctaa gatatggcta gattcctgtt ctgcactagc
     4381 tgcatacaga agtagctctt ctttttttat tccattgact ggctgaatcg cctctacttc
     4441 caatatccct ttggtaagcg ttcctgtttt atcaaaagca atcgtttttg tacgtgcgag
     4501 cttttcaatc gttgttccag tttttacaac gatcccgttt ttacttgaac ggctcattcc
     4561 tgcaactaaa gcaatgggcg cagctagaat cagtggacaa ggtgatgcca ctaccagtac
     4621 ctcagcaaat cgtaaagggt cttttgagaa ccaccaagaa ataccaccga tcaaataagc
     4681 aatcaacgtg aaagggactg cataacgatc agctaaacgg acaaatcggg caggcttttc
     4741 ttttgattct tcgactagtt taataatcat ttgatactgg ctgtctgcgg cacgtttttc
     4801 agcgatcatt tggatcgatc cgtctccatt taaagaccct gacataatct gatctccagg
     4861 tttcttggtt tccgggcggg actcacctgt caaagaagat tcatcgaccg aagattctcc
     4921 tttggtcact cgaccatctg cgggtacaag ttcgccaggc ttgactagta actggtcatc
     4981 aatatttatt tcatcgatta ctatatcctg caattttcct tctttgaatc gatgggcaat
     5041 tcttggggtg ttatctaaca aagactgcag ttctttaccc gctcgtttat tcgcgtaatc
     5101 ctctagacta tctccgcccg taagcatgat caatatcatc aaactagccc agtattcttt
     5161 gacggctaat gttgcaagga ttgcggtaat cgctaataca tctacgccat atcttcctga
     5221 acgtaacgtc ttgatcattt ccaaaaacat caagaaggcg agaatccctc cagtaaatgc
     5281 gacaatcgca aatgcaaggg ccggctggtg aaaaacaaat tcacttaata aagctatccc
     5341 accaataagc aaagtaagta aaaatttttt gaaatgtgtc attgtttttt ctcctctttt
     5401 atgtgactta gagaggttag taataaattt ccagctgcct ctttcatctt aagcatagtc
     5461 cttgactagc actttttaaa acaataggcc ttaattgaaa acaactatca ctatcagcgt
     5521 ttccaaaatt catcagttct acaaacaaaa atattcagca aggattactt tttcgattca
     5581 tcagttaaaa agtacctttt ttcgaaaaac tatgctatac ttttaattcg ttatttacgg
     5641 cggaaggagc aaacatgcga tgaatgagaa acaactagaa caaacgcacg tggatgaaac
     5701 gatcgaacta atccagatcg aacaaaatat gctggataca aaacaacaag aattgactca
     5761 ggaaatgaat cattcgacaa aagacacagc taatcacaag attcgagctg gttccaatga
     5821 agctttttat gaatctgctg ttgaatatcg ccaacatgag caggaattgc ttttgaaata
     5881 ccatactctt gaagcacagc aaaaacgcct gcaaacgttg tctgtcatga aagatagtcc
     5941 ttattttgcc cgtatcgact ttaaagaaga ccgtgaaaca gaaacattat atctaggtat
     6001 cgcttcttta agagatctgg cagaagaaac aatcgttatt gactggcgcg caccaattgc
     6061 caatctttat tatgaaggtg agattggtcc cgcattttat gaaactgaaa cagatagaat
     6121 agatgtcgat ctgcagttga aacgacaatt taaaatccaa gaaggacgta ttttatctat
     6181 ggtagatacg tcggaagtaa tcaatgatga ctttttgcta gagatattgg atgaatcatc
     6241 aagcaaccat atgaaaaaca tcgtctctac gatacaaaaa gcacaaaatg caatcattcg
     6301 ggacacaaca agtaaagtaa tgctgattga aggaatcgcc ggaagtggaa agacatccgc
     6361 tttgcttcaa cgtattgctt ttctgttgta tcataatcgc aagtggctcg aggctgaaaa
     6421 cgttcttcta ttttcaccca accatttgtt ttctgattac atctcgactg tattgccttc
     6481 attaggtgaa agcggcgtgc ctacacagac attcaaaaat tatttggtgc agctgctgcc
     6541 agagttttct ttactagatg aacagcaaca agaaagtggt tttttgctag gggaaaaaga
     6601 tccgatacaa gtgatgaaaa gcggattgac cttagtcgat cagattgatc gctatatcca
     6661 aggcatcaca aactacggtc ccttgttccg tgatatgaaa ataaatggcc gtacgatcct
     6721 atcaaaggaa tcgattcgcc aatggtataa agaaacaaat gaacaattac ctttacatca
     6781 tagactttct ctattacaaa cgaagctcct gaaaaaatta ggcggtctgc aaaaagacga
     6841 aacacgtcag caatgggtca aagatctggc ggaagaacag ctccaagaac tatacgcaac
     6901 agatccaaat cttgaatata cagaacaaaa ggagcgttca ttacgtaaaa aactggccaa
     6961 ccaaatcgta cgtaaaagat tccgtcgaat ccatcgaggg atcatgaatt atcaatttgt
     7021 caatataacg aaacaatatc tccactttct acaaactgtg ccactttctc ttctaacacg
     7081 acataaaatc agcgagacca ggtggcagga acatttgaca actgttcgta cacacttgcg
     7141 tcaaagagaa ttgcgtcaag aggatgctgt actattcttt ttactgatgc gccgaacata
     7201 tccagtgacg gttacacaca aagctcgcta tatctttatt gatgaaatgc aggatttcgc
     7261 accatcacaa gtggcattgc tgcaagcctt gttcccaaaa gcaaatttga ctttctgtgg
     7321 agatctaaat caaaatgtct ttggaaatga aacgatcgtt ggatcattgg aagacctttt
     7381 cgtggaccgt gaagtcactc gtttccaatt aacgacaagc tatcgctcca caaaagaaat
     7441 cactgatttt gctaatcatt ttattgtgaa agaacatcaa gtagaaacaa cagcaagaaa
     7501 aggagacctt cctttgctca tccaaggaac aacagatgat atcaaattga attggttagc
     7561 agataccatt cgaacgactc agggaaaagc ccggtattgg cgtacagcaa tcatagctaa
     7621 aacacaagaa gaatgcgaag ctctttatgc tcaactgcca gacgagttga aacaacaagt
     7681 ccagctgatt ttaaatgaga gtgactttat gaagcgttcc attattttac ttccagcctt
     7741 tttagcaaaa ggtctggagt ttgaccgagt tttcttgtgg gatgtagatg cagaacattt
     7801 ttcttctcag caagacaaat tgatccttta caccatgtgt acacgtgcga tgcatgaatt
     7861 gacccttttt acctccaata atgaaagtcc tttgttaaac aatatggata tttcatctta
     7921 tgagaaaaaa gtactgtaag taaaaagaca actgctatca ttaatgttag cagttgtctt
     7981 tttatttaca ataataattg cagaaaaagt aattcaatca tttaaggtca tttattctat
     8041 atataaatcc aaatctgttt caggatattt taatttgaca agcgaaataa tcattttcag
     8101 ttcttccatg ttttctggat attctgggtc aaataccttg ctatgacggc caatcccgcc
     8161 attgtcgatc aatcggtaaa ttcgattcca ttcaatgaat tggtcaattg attggaaatt
     8221 gctgattgga tagccgccac aatccaaaat ctctgataca cgaaatgcta ggtatgcact
     8281 atcgatcact ttattttttt tcgtgtctat cttctcattt ctttcgataa gaattttttc
     8341 aggacgatgt ttcaagctcc actctaatag tagaaagaaa gcctccatcc gatactcttc
     8401 ttcaaataac cgaaaggctg aatcagaacg acgaaaaagc agcggaatca gataattttg
     8461 tctataaatc agcattggtg cgcggtccac catacactct aaataacggt tcagataccc
     8521 tgtaccgaca tagtttccta atcgatagcc ttcaacgccg aatcttgcgt atttttgcgc
     8581 taattttgaa caatcagtta cgttcacagc taactcttct actttgcggt aaccgcttaa
     8641 gatacgttca aaaggttcta aagtgatttt tgcttccata ttcttccccg tccatcttcc
     8701 acatacttca tatcatttca catgatactt atgctctcat cataccacag gttgaattag
     8761 aaaagaaaat aaatcacaca aaaaagcaag acaatctgtt agtgagattg tcttgctttt
     8821 cgaaaaagac tgaatacttt tcgttaaaac tggttgctta gtcttctaat gccacattat
     8881 ggaacacatg ctgtacatct tctagatctt ccagtgcatc aatcattttt tcaaatgttt
     8941 cttgatcttc tggagatagt gttacctcgt tttgagggat catttggatc tcagcgattg
     9001 aaaaatcagt gattcctttt tcttttaatg cttcttgtat actatggaaa tccgctactt
     9061 caccgtaaac gatgatttgt ccttcttctt ccattacatc gcgaacatcg atgtcttttt
     9121 ccattaggta ttcaaggatc tcgtcagcgt cttctcctgc aaaagcaaag attgctgtat
     9181 tgtcaaacat gtaagaaacc gctccggaaa cacccatgtt cccgccattt ttcccaaatg
     9241 ctgcccgtac gtctgctgct gttcgattca cattgttcgt caatgtatca acaatcacca
     9301 ttgagccgct tggtccaaat ccttcatagc gcaattcaga gtatgtttca tctcctgcac
     9361 cttttgcttt ttcgattgct cggtcaataa tatgttttgg tacattataa gttttagcgc
     9421 gatcaatgac gaaacgtagt ttttggtttg catgcggatc aggatcacct gattttgctg
     9481 ccgcgtagat ttcaatccca aattttgcat aaatccggct attattagca tctttagcag
     9541 ttttctttgc tacgatattt gcccatttac gtcccatatt ttcacttcgc tttctgattt
     9601 gatagtttca taacgcaaaa acgtcctgtt ccattatact tgtgaatcac gattctgtga
     9661 agcttttttt ctttcttcct tcattcttct catttttagt caattatcta tactcggatt
     9721 gggatttgcc cgaaattctg ttccggaata ttgcctccca attgatagat ttgatcactt
     9781 ctaactaaca gttccgcttc ttgtgcctct ttttttccga ttcgggaata aatagaaatc
     9841 tctttttttt cttttaagaa tcttcttcca gcatcggtaa atccaagtag tcgaagataa
     9901 tttttatttt gttggacagg aatctcttgc tttttgatat tcagcaagac ataacaagat
     9961 aatcgttgga tccgtgtcca tgtatatctt tttgttttta cctttgacac aaaatcctca
    10021 aaactatcag cagttgcagc tgctgttttc atgcgcgatt ctattccttc ggtcatctga
    10081 tagatatctg tcagttccag caaagtagaa gataacaacc gataacgcaa atacggccaa
    10141 taagattccc aagtctgcag cgaacaagtt gaaagatctt cttctgtctg aggcggcaca
    10201 agtccagcta ttctttttcc ttcttctaac gcatggcgaa tggctgttgc actggctatc
    10261 ctctgatctg tgatgatttt ttcatgatag cctgcacttt tacgtcggat cggatgtaat
    10321 tccatagggt ggacatactt ggcattttcc tttgcataac ttaatcccaa gatatgattt
    10381 ggcgaagaaa aatcaagcgt aatatcggga tacagactgc gaaaaacctc agtcattttt
    10441 tgcggataac tatacgtttg atcagacagt tcttgaaatg cctcttctat cctatcttga
    10501 ttctcttgga caaagcgtcc gaaactgctg taatcaaacg aagtctcttc ttctgttcca
    10561 aaacacaaac tgtcacagtc cagtgtttgc aagattttta ctgcaccttt agcaaaatag
    10621 tcagctgact gaaccgccca ttcgacggga agctccacta ctaaatccac accattttgt
    10681 aaagcttcat tcgcgcgatg ccatttatcg atgatagccg gttcgcctct ttgcaaaaaa
    10741 ttcccgctca ttacagcaat gacgacctct gctcctgtta tttttcgtgc catttccaca
    10801 tgatagcgat gtccattgtg aaaaggattg tactctacga taatcccgca gcttttcatg
    10861 cgcatcctcc ttcatagtta tcgataaaaa agaaatagag acaagtaaaa aaaggcaaaa
    10921 atcggaaaaa atacccgaac tatataaact attctcgcaa acacaatact tctgcgacga
    10981 aaagctgcac tgcaagagca atgcctatat cgttcggata tataatttat gttgaagatt
    11041 tattcttttc ttgttaactc gagcaggcaa aaccgctagt ctttgtatgc tacaaagaac
    11101 cagcgctttg atttctctga tgctgcttcg tctgtaaaat cagaaaatgc ttcgactttc
    11161 gaaaagccgc tgttttccag cattcttaaa taactttcca atggataggt acgctcttga
    11221 tgtagttcat cttcacgaat aaatcgacca gacccttctt gttcttcaac gaaaaaggta
    11281 agaaaatgtt cgatgctgtg tttctcgctt cctggataac tatcccacaa aaaagcaaaa
    11341 ttatcccctt gataatgata gctatattct ggaaaaacct catctacttg ataggtggag
    11401 tgtacgtcaa ataaaaatcg gccattttct tctaacgctt gatagacttc atcaaacact
    11461 tgctgtacct cttgtctatt tttcatatag cacagcgaat cagaaaaaca ggtaattgcc
    11521 tgatattgtc cgatttctga taaatcgagc atattgcctt ccacaaattg gatcgatgcg
    11581 tcttcttcat atgctcgctg gctggcaatc atcaacatct cttctgaaag gtctaaccct
    11641 gtcactttat atccatcttt tgcaaaggca accgctaatg ctccagtacc gcatgccagt
    11701 tccaaaatat tttttgtgtc ttcttgtaaa tgacgtttcg aaaaagctaa ccattgctga
    11761 taaagtgttt cgtccatcac ttcatcgtaa atgaaagcaa atgtttcata agccatatgt
    11821 taatccaacc attcactaag atcgactagc ggagcatctg accatagttt ttccagatta
    11881 tagaaatttc gttctggcgc atggaaaaca tgtacgatca catctccaag atcgatcaat
    11941 acccattttc cgccttcttt tccttcaata cgttttactt catatttgtt ttcttcttct
    12001 ttatcaatga tttcttctgt aattgcgttg atttgacgtt cgctatttgc tgaacagatc
    12061 atgaaataat ctgccaacaa ggaaacattt cgaacgtcta aagcgataat gtcttctgcc
    12121 cgttttgaat ctgctgcttc aatcgcaatt tttaattgtt ctttactatc tatgatgatt
    12181 tcctcctaat atgagcagct tatttttttg ctacccagta attatatgtt tcgattgttt
    12241 ttggataaat ctgttgttct tgcgcaagca aatgggccaa tgtatgtttt gtttcaaatg
    12301 ctacagcttc gtctagatca gcccatgcaa tggctcgtgc atcttgtacc ccaggaaaat
    12361 ctcgacctgg ttcaatatag tctgctacat aaatgatttt gtcaaggaga ctcatcttag
    12421 cagccccagt cgtatgcacc cggattgcat gaagaatctc tgcatcggtg attcctaatt
    12481 cccgctctac aaacgaagcg ccaacaagac catgccagat tgcattatta taattcaata
    12541 actccggatc aaaaccgtct cttacgatta ccattttgaa ctcctcatct ggtcgttctt
    12601 ttgcataatc atgtgtcaat gcagcaatac tagctttttc aggagaagca ccgtattttt
    12661 tggcaagagc cacggctgtt tcttctactc caagcacatg cttgaagcgt tgttcgctca
    12721 ttctcatctg tattttttgc atcaggtttt ctctagaata agcagtatat tcattactat
    12781 agtccatatt catacaaccc ttcattttga atataatcaa ttactttatc tggaacaagg
    12841 tagcggatag aacagccttc tttgatcttt tgacgtattt tcgtggaact gatgtcgatc
    12901 tcaggtacat ctacccaaat tacaggatat ggagtatccg ttgtgtatcc tggtcgacgt
    12961 atacccacaa agttgaccat agaagtcagc tcatcgattt tataccattt tggcaaatat
    13021 tctaccatat ccccaccgat aataaagtaa taatctgtat ccggattgtt ttgcgtcaat
    13081 tccttcatag tatcataggt gtaacttttc ccaccacgag ctaattcaat cgtttcaatc
    13141 tgcaaaaaag gattgtcttc caccgctaat tccaacatgt tgagtcgatg ttttgcatca
    13201 atcgtctgtt tctcatcaac atgaggtggc tgataagaag gcatcaaaaa tacgcggtca
    13261 agacctaaat tccggccagc ttgttcagcc atcactaaat gtgcaaggtg aacgggattg
    13321 aatgttccgc ccaagatccc tacttgcttt ttcttctcta agaattgagg catttcttca
    13381 ggaaataatt gtgaacgaac aaatgtctta gcttgcgtat tcatcatggt cactccttat
    13441 attgctttta cttcttttga tagcctttga tacttttctt ttgtggaagg tttgtataat
    13501 acaataacac gtccaatgat ttggacgatt tcgcaatcta tttctttttc taatacttga
    13561 gctgcttctt ctgcaatctc atctgtattt tgaagtaacg atactttgat cagttctctt
    13621 ttttctaaag cttcttcaat ttgaacgatc attgctgaat tgatcccacc ttttccgatt
    13681 tgaaaaatcg gctgaagatg atgcgctttg ctacgtaaaa aacgcttttg ttttcctctt
    13741 aaactcataa ttttccttct tccttaaatc aatgctttgc gtttcaatac atctacaccc
    13801 tttggcgccc agcccgcaat tactactggc gctgttacag tgatccagcc tagaccggca
    13861 aaaacgatgt ctgttttttc tttcacagaa aactcaaaac gaacaagctc cggaaatgcc
    13921 tcgacttcat caggacgagg aggctgtaat aatccaccta catgtttttg ataaaattcg
    13981 tctgcttttt ccagtttcgt tcgatgaata tttacatcat ttgacacata agcaataaat
    14041 gaagcacgat tgccgctgat aaaatcaaat cttgcaagtc cgcccaagaa aagtgtttgc
    14101 ccttcattca gctgataaac tttaggtttg atttctttta acggtgcaat gatccgcaaa
    14161 tcttttttac ctaagtagtg cgccatttga tggcgatgga taattcccgg tgtatcaatc
    14221 aaaaaatgcc cgtcatctaa aggaatctcg attttatcca aggtcgttcc tggaaactgc
    14281 gaagttgtga ttacatcttg tactcccgct gtatttttga tgatttgatt gataagcgtc
    14341 gattttccga catttgttac tccgacgata tagacatccc gtccatcgcg gtattcttcg
    14401 attttttcta atagttccgt catctcatga gatttcttag cactagttaa taatacatct
    14461 actggacgaa ttcctgcttc gtaagcacgt tccttcatcc attgtgttaa tttagttttt
    14521 ttcaatgatt taggtaatat atccactttg ttcccaacta aaagtactgg attgtcaccg
    14581 acaaaacggt gtagtcctgg gattagtgaa ccattgaagt caaaaatatc caccacattg
    14641 acgatcaatg catctttttg ccctaattca ttcagtaagc gcaaaaagtc gtcatccgtc
    14701 aatgagacat cttgggtctc attataatgg cgtaatctga aacaacgctg acaatagact
    14761 tccccggaag ctaaaccttt ttccaaagcc gtttgcggcg tgtatcctaa ttcaccgggt
    14821 ttgtttgttt gtataacggc accacagccg atacattgta attcttctgt cattcgattc
    14881 cacctcgcca gcccatatca gggtgttttg ctaatagata ggccataatt cttttttccc
    14941 aaaaacgatt aaattgtgtc ttccaactat ctgttgcgat aatgggtttt acaaggatgc
    15001 tacgtatccc tgcacgattg gctccacgta tatcagtcat aatctgatcg cctaccatta
    15061 ctatctcatc atctgctaaa tccaattgtt tctttgctat gttgatccct cttgctaaag
    15121 gtttcattgc tcgtgcaaca tacaaaagat caaatttttc aatcgctcgc gcgacacgtt
    15181 ttgaattatt gtttgaaaca acaacaacag ggattcctgc atttttcatt tctaaaatcc
    15241 aagtaagcaa ctcttctgtt ccatctggat tattccatgc aatcaacgta ttgtctaaat
    15301 cggtcaatac tgctttgatg cctagattct ttaattgggc aggtgtaatc tgatagatgg
    15361 cttctaccat ccatgttggt ttgtaatttg aaaacatgct gtctcctcat ctgcaaaaaa
    15421 tgaaggagca cataaagtac tcctcaaccc cgtattatac tgcatatcag caaaaaaaac
    15481 tacctctata ctcgttcgtc tttcagaaaa agcatttctt tacaaaatga ccaaaaataa
    15541 agattgatca cagaaaaatt tagaaacaaa aaacaactaa tattggcgga acctcttgta
    15601 tcgtttttcc agtaatttat gctgatctaa tcgaacaagt tccgtcaatc tttcaatcaa
    15661 cgcttttttc ataatgtgtg cgactttata tcgtggcaac tgctcgccgt tgaacgtttc
    15721 aggaataact cgatcaacga ttcctaactc gcgtagttct ggcgcagtaa ttttcatcaa
    15781 ctcagctgct tctgcggcgc gactaccgtc tttccacaaa atcgacgcaa atccttctgg
    15841 agaaagcaca gcataaatcg tttcttccag catccagact tcatccgcta aacctagagc
    15901 aagggcaccg ccacttccac cttcaccaat gatgatggcg atgatcggta cctttaaatc
    15961 agacatttcg agcagatttc tggcaatagc ctctccttcg ccgcgctctt ctgctcctac
    16021 accgcaatat gcacctgctg tattgatcaa ggtaatcacc ggtctattga acttttccgc
    16081 ttgtttcatc agtcgtaaag cttttcgata gccttcagga tggggagaac caaaatttct
    16141 gcggatattt tcctgcagat cccgtccttt ttgaataccg atcactgtta ccggtttgcc
    16201 atctaatcga gcgattccac caacaatcgc ttcgtcatct gcaaataaac gatcaccatg
    16261 gcattcctcg aaagaatcga agatctcttc aaaaaaatcc aagcttgtcc atcgttcttt
    16321 agctcttgct aactgaacga catcatgtgc tgtcttatcc acgatctatc catcctttct
    16381 gtgtatgcaa ttcaagaagc gtatggattt tctgtttcaa ttccatgcgg ggaacaatct
    16441 gatcgacaaa accatgagat aataagaatt cagccttttg gaaatcttct ggaagttcct
    16501 gcttgatcgt ctgttcgatg acgcgtcttc cagcaaaacc gatcaagctt tggggttctg
    16561 ctagaataat atcgccttcc attgcaaaac tggcagttac tcctccggtc gtcgggtccg
    16621 ttaaaacagt tagataaaaa agaccttcat tgctatgccg tttgacagca gcagagattt
    16681 ttgccatctg cattagagaa aagattcctt cttgcatccg tgcgccacct gaagctgtga
    16741 acaagacgac tggaagactt tcctttgttg ctcgttcgaa taatctggtg atcttttctc
    16801 cgacaatcgt tcccatactt cccatgataa aattaggatc catcactcca ataatgaagg
    16861 gaatcccctg aatggttgct ttaccagtga ggactgcttc atgaagtcct gttttatctt
    16921 gcattttttt aattttttcc gtatattcgg gaaatgctag cggatctttg gttacaagat
    16981 ccgtatccca ttcttcaaaa cttttttcat caataatcaa agctagacgc tgccatgcac
    17041 cgattcgaaa agcgtatccg caatgagggc atactttttc ttcaccgatg tctttcgtat
    17101 aaaggatatg tttgcaattt ggacactttg cccacatgtt atcaggaaca gatggcgagt
    17161 tagcaggttt gctctctcta ttaggattga tccgaatata tttctttttc ttaaacagag
    17221 ccatctatgc gcctcctatc tccgtttctg gtgtccaatt cggtaaaaat tcttcttgta
    17281 aaaatgctgt gctgtaatca cctgctatga ctgcaggatg actaatcaaa tccatctgaa
    17341 attctgcgtt cgtgatgatt ccttctgtga taaattcact taaggctcgc tgcatcttca
    17401 ttaatgcttc aaaacgcgta tttccatgga cgattatttt tgcaatcatt gaatcataat
    17461 atggcgggat cgtatatccc gggtaaactg cactgtctac tcgcagtccc atcccacctg
    17521 ctggaagcag taagttttgg atcttccctg gagaaggagc aaaatggaaa gcaggatttt
    17581 ctgcattaat ccggcattcg attgcgtgtc cttggaatac tacttcttct tgacacacat
    17641 tcaaaggctc accggctgct atctccacct gtttttttac taaatcgatt cctgtgacca
    17701 tttctgttac tggatgttcc acttggattc gagtattcat ttccatgaaa taaaaatcgc
    17761 cttcttcatc catcagaaac tcgatcgttc cggcattttc ataattgact gctttagctg
    17821 ctcgaacagc agcgtctcct agtgcttctc tttttgtttg tgagatcact acagaaggag
    17881 attcttccag tactttttga ttgttgcgtt gtaaagaaca atctcgctcg cctaaatgaa
    17941 tgacatgacc aaatcgatca cctaagatct gaacttctat atggcgagca ggataaatga
    18001 ttttttccaa atacatatca tcatttccaa atgccgctgc tgcttcttgc tgagctgaag
    18061 aaaaatgctg tgggagctct tcttttgatt gaactttccg aatgcctttc ccgccaccac
    18121 ctgcagctgc tttcagcatc actggataac caattctgtc tgctatttcc aatgcttctt
    18181 ctactgtaga taatacacca gtacttcctg gaatgactgg gacatttgct tcttgcatca
    18241 attgacgcgc attgatctta tttcccattg catcgatcgt tgcactttta ggaccaataa
    18301 atgtgatatt gcactcttca cacatggagg caaatcggct attttcagat aaaaaaccaa
    18361 atcctggatg aattgcttcg gcttttgtca cgattgcagc gctcagtact tcctgaacat
    18421 tcagatatga atcaacggct tttgccgggc caatgcagat tgcttcatct gctaactgtg
    18481 tatgaagtgc ttcggcatct gcttctgaat atatcgctac ggtttggatc cctagttcgc
    18541 ggcatgcacg aatgatacgt acagcgattt caccgcggtt agctatcaat actttagaaa
    18601 acatatttca cctatccaat cataaaggtt aattccgctt cggctacttt tttaccgtct
    18661 actttagcga ttcctttgcc tattccggct gaagctttca ctttcaatat ctcaacttca
    18721 aggtataagg tatctcccgg tgtaactttc ttacggaatt ttgctttgtc caatccgccg
    18781 aaataagccg tctttccctt gaattgttca agagaaagta atgcaactgc tcctgcttga
    18841 gccattgctt caacaatcaa tacccctggc ataacaggtt cttctggaaa atgtccttga
    18901 aaaaagggct cattgatcgt tacatttttc ttagcaacga tccgttctcc ttcaaccatt
    18961 tcttccactc gatcaatcaa aagcattgga taacgatgtg gaatgatttc ttttatttct
    19021 tgtatgttca ttgttttccc tctttcctgc tttgcttatt tttgctgaat cctaaagagt
    19081 ggctgtccat attcgacaac tgactcgttt tctacaagga tttctgtgat cactccgtcg
    19141 gcctcactag ttatctcatt catcagtttc atcgcttcaa taatgcagat aacttctcct
    19201 ttggcaactt gatcacctac ttttttgaaa gctggtttgt cgggcgctgg ttgcagataa
    19261 acgattccca cgattggcga aacaacctcg attccatcaa atactgccga ttctttttct
    19321 ttcattattt ctggagcagt tgttgcttgt tcaggtatga cattgactgg ctccgaaact
    19381 ggcaacgttt cgtttgtttg aaccatattc atgcctcgac ttgttttatt tttattcata
    19441 tacaattcaa attgaccttc acgcaaatcg aattccgtta gcgaggattg atcaaattga
    19501 ctgactaatt ctctgatttc attgatatcc aaaattattc ctcccagcgt ttcatgcaca
    19561 gaaccgcatt atgtccgcca aaccctaatg aattactcat tgcataagta aaacttgcag
    19621 gtttgctttc gtttttgaca actgtcaagt caatctcagg atcttgtttt tctacattga
    19681 tcgtaggtgg gataaattga tgttctaatg ccagcaaagt tgccaaggct tcgatcccgc
    19741 cggctgctcc caataaatgt ccagtcatcg atttcgtgct gctgacgtaa gtatctctga
    19801 aattttcacc taatgcgatt tggattgctt ttgcttctgc ctggtcattg gctggtgtac
    19861 ttgttccgtg agcattgata tatccgactt gttccggctg tacaccagct tcttccatcg
    19921 caagcttcat tgctttagat gcaccagaac catctggagt aggagctgtc atatggtaag
    19981 cgtcacagtt tgcaccgtat ccaacgattt ctccatagat tttggcaccg cgagcttgtg
    20041 catgttctaa agattcaaga actagaacgc ctgctccttc acccatcaca aaaccattcc
    20101 gattttcgtc aaacggaatc gaaccacgat taggatcagt tgattctgag agtgctgtta
    20161 aagctgcaaa tccagcaatc cccatctcac aaatcgttgc ttctgttcct ccagcaagaa
    20221 tgacatcaga atagccatgt ttgatgttac ggaatgcttc cccaattgaa ttcgttccgc
    20281 tagcacaagc tgttacgata gctgtacaaa taccttttgc acctacacgc aaagcaatat
    20341 ttccagctgc catgttggca atcgccattg gtacgaacat tggagaaact cgttttgcac
    20401 ctttttcatg tagacgaata acttggtttt cgattgttgg aagaccaccg atcccagatc
    20461 cgatgatgac gccaaaccgg tcttgattca ttttttcttt atctaaaccg ctcatctcca
    20521 ttgcttctaa agcagcgtag attccataga tcgaaaacat atccattcgt ttgctatctt
    20581 ttttaataaa atatttatcg taagggaaat ctttgacttc acccgctaag gtaatccctg
    20641 tttctgaagc atcaaattta gtgatcggtg cgatgccatt cgtcccctct tttaagctat
    20701 tcaaaaagct gtctgcgtca tttccaatag gtgaagtgac tccatagcca gtaattacca
    20761 ctcgattcat ttgtttgctc tccttttctt atccgtgcat gaccaatccg ccatcgacat
    20821 tgatgacttg tccggtgata tatggatttt gtgctaagaa aactgctgtt tgtgcaatat
    20881 cttctacttg tccgaaacgc tgcatcggaa tttgcttttc cgcttgttcc ttgactttgt
    20941 ctgctaaaac ttccgtcata tcagttgtga tgaatcctgg cgcaatggca ttacatgtaa
    21001 tgcctctagt tgcagcttct cgtgccacag attttgttaa tcctactacg ccagctttac
    21061 tagctgcata atttgcttga ccaatatttc caatcaaccc agatacacta gagagattaa
    21121 tgatggcccc ttctcgctgc ttcatcattt ttttcaatac gtgctgcgtc atattgaagg
    21181 ttccaatcaa attgatatcc agacattttt tgaaatcttc tgcatccatt cgcatcacaa
    21241 gtttgtcatt tgtgattccg gcattgttta ctaacacatg gatcgatcca agcttttctt
    21301 ctgcttcttt gatcatttgt cccgctttct cataatcaga gatgtcaccg gaaactccta
    21361 cgcattttac accgaatgct tcgatttctt ctatcttttc ttttggaatc tctccgcgac
    21421 cattcaaaat gatatttgct ccagcttttg caaaagctaa ggccatcgct ttgccgattc
    21481 ctcttgtact acctgtgata aatacatttt ttccttttac atccataacg ttcctcctta
    21541 tagtattgct attgtttcat caaatgtctg cttattctca acacgcagaa ctggtattgt
    21601 tttatcgatt tttttcataa atccgcttaa tacttttccg gggccgactt cgatgacttg
    21661 ttcgatgccg attgttttta acttatggat actctcatag aaacgaacag gttgcatgac
    21721 ttgctgttca agtagtcccg cgattgtttc tttcttcatt atttccgttg ttgtgttgct
    21781 tatgatagga aaagaaggtt cagagaattg aatctgactt aggtccttcg ccagtttttt
    21841 tgctgccggt tctaaaatag ctgtatgaaa aggcccgctg acatttagag ggatcattct
    21901 tttgaaccct ttttctttta atagtaaaac agcttcgtct actgcttttt cttcgccacc
    21961 aatcacgatc tgctgaggtg tattataatt tgcaggagat acgatgccgt acttgctagc
    22021 ttcatgacag ctttcctcga tcgtttcaat cggcgcattc atcactgcaa ccattttccc
    22081 gcttccagct ggggcagctt ctgtcatata agcgcctctt tttgctacta acgcaactgc
    22141 ttcagtgaag gatagcgcat tactagctac taatgccgaa tattctccta aactcagtcc
    22201 taaagctgca tctgggatga ttccatgctc cttcaataag cgataatacg cgatgctgac
    22261 tgttagaata gctggctgtg tatattgcgt ctgatccaaa cgttcatttt ctgtaaagca
    22321 aagttcagcc atatcgtagc cgagaatctc acttgcttca tcaaatgtct gctttacaac
    22381 ggcttcttca tataaatctt ttcccatacc ttgatattgt gcaccttggc cactaaataa
    22441 gaatgctgtt ttcatgtttt tctccaatat atccagaata tatgtttgat tcttcctatt
    22501 caaaaaggcc gagacattcc tatttcagga aatgtcccag cccccaacct aattttaatg
    22561 attatgcagt ttgtttttct acgtaagtca ctaaatcttg aaccgttttg attccgtctt
    22621 ctgtttcgat tttcacgtca aattcatctt caatttcatt gatgatttgg aataaatcca
    22681 agctgtcagc ttctaagtct tcttggatgt cagtagtcaa tttgatttct tcagtttctt
    22741 ttcctaattc ttctgcgatg atttcttgaa ttttttcgaa taccataata atatattcct
    22801 ccgatattgt ttgcttttta tttttataat ttaacagtct acaactccat gagaatactt
    22861 ccccatgtta aacctccgcc atagcctgta aatattactt tctgctgttt tcctaaggtg
    22921 atctttccac tagaaactgc ttcatccaat aagataggga tgcttgcagc agatgtattc
    22981 ccatatttat ccatattggt caggaacttt tcttgcggca tctttacttt tcgggcaatt
    23041 ttctcaataa ttctgaggtt tgcctgatgc aaaagcagat aatcaactgg tttgtctttc
    23101 atgacttctt ggatattgtt tgcaacgtct cgtacggcaa aatcaaaaat atctctgcca
    23161 gtcatttgca aataatacga accttctgaa tcttctgaat agaacggatt tttattttgc
    23221 tggtagccag cggtcaatga gctacttctg gtaccgtcag ctgctaattt ttctttcagc
    23281 aattttttct gtggattcgc ttctaagaga atcccacctg ctccatcgcc aaacaacaca
    23341 gcagtcgtac gatcagacca atcgattagc ttagatagtg tttcaccacc aatcaccagt
    23401 cccttagttg agccgcagcg gatcattttt tcagccatgc ttaacgcata aacaaatcct
    23461 gagcacgcag cactgatatc aaaagcgata gcatgagttg ctcccaattt cccttgtact
    23521 tgtgctgcta cagatggtgt cgtgtaatcg ggcgacattg ttgctactaa aataaaatcc
    23581 aaatctttag ggtttagatt tttctgtttt atcaattttt ccgccactaa aatgcataaa
    23641 tcagaagtct cctgatttgt tacacatctt cgttcacgaa tacccgtcct tgacgaaatc
    23701 cattcatcag aggtatccat catcttggaa agatcattat tagtaataat cttttcagga
    23761 aggacgctgg ctgttgcagt aatcattcca aattgttcca ttaaatatcc tcacttatag
    23821 tcttgcaaga agtcgtgcaa gtttttcagt gcatccagca aggcatgctg ttcctccgta
    23881 gccattccgt ctaagatgtt cttgaccatt tcacgatgaa aatgctgatg gacccgaaat
    23941 agtaactttc cttttttcgt tagtcccaac tttacaacgc ggcggtcatc ttcactgcgg
    24001 atacgttcga catagccttt ttttactaac ttgttaatag cagtggtcaa ggtcccaaca
    24061 gtaattgata gttctttggc tacttctgaa gttgtttttt tcttgtacat cccgattgcc
    24121 tcgatcgtgt gcatctctgt gattgacaga tctttgaatt gagatttttt cagctcagac
    24181 tcttcgattg tcaaaatatc attgaagaca ctaacaagat aatcatttac tgtttttaaa
    24241 tctggttcca tgttccaccg tccactaatt agtttgatta tcaaatcttt tgatgttcaa
    24301 actatatctt aacttttttc aaaatgcaac cttaaaagat cattttttta ctatatgagc
    24361 tttttttaag attttactca tttaaaacaa ctgataaatc agcatttata atttctttta
    24421 ttagaaaaat catctatctc acatattttg attatcaaag tattttctgt gaacagaact
    24481 atagaattcc ctaaaacttg tccattattt caaaatttgt tatagtcttt ttattcaaag
    24541 cgtaaaagga gatcctatgt taaaagaaaa aatgaaaact ctccccatgc tgccgggtgt
    24601 ctatcttatg aaagaccgag atggtaaggt catctatgtt ggtaaagcaa aacggctcaa
    24661 aaatagagta agcagctatt ttcatcagaa taaacagcat tcaaaaaaag tattacgtat
    24721 gattcatcat ataacagatt ttgatttcgt cgtagtagac acggaattag atgctttatt
    24781 gctcgagtgt caattgatcc aacattaccg tcctttttat aaccgacaaa tgaattattt
    24841 cagtaattat aattatgtac atattaccaa taagggattt gtattaacag atactcctac
    24901 ggctcgaaca tacgggcctt ttcgtctcta taaaaaaatg ccgtctattt tgcgtataat
    24961 ggaagaaact tatcaaatgc cttggttgtc tgaaattagt ttgttagctt tgcgagtcca
    25021 gcttcctgac ctacaggaaa tgacttttga acagaaaaaa aaggaactcc aagggctgtt
    25081 tcaaggccga aataaaaagc tactgactta cttgaaaaaa agacaacaac atttcatcta
    25141 tcagttgaat ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag ttacttattt
    25201 tattagacgg atccaggaac aaaagcagtt tttaagaaca ccatcgctca ccttcagcat
    25261 gccattagca gcagatgaga gtcagaaaaa gcactacctc gtttgttatg gtcaacttgc
    25321 agaaacaatg attacctctg gtgacgtttc tcctgacttc tattatgaaa agaaagaagc
    25381 tcatttatct ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac aaatcttgat
    25441 cagttaccgc aaaaaattag aaaaagaaca gtttgaaatt gaactgttaa acaaaaaaga
    25501 agctgaaaag caactaaatt agttgcttct cggcttcttt taggcatctt caacttcaaa
    25561 tgtcaattat cctaaaattt attggattaa gtcatagatt tccatcgcta cgatatcgat
    25621 attatcaaat tcgtattgct gatgagtatc tgtttcagtt aattcaaatg tatcggtcgc
    25681 tttatcataa gttactgtac acttctctac gccttctttt tcaaaacggc gaacttgcag
    25741 ttcattatct gctgactcga ccatagcctc cagtcgtttt atgattgcga ctaattgtga
    25801 atgtgtcatc ggaggtcctc cttttcttat ttctgttata ttgtatcaaa agcatagcct
    25861 acttgcattg attttctcta aaaaaactcg ttttttatga ttttttttct ggtatttttc
    25921 cttcaggcca ccaaagtttg atcaaagctt gggttccgtc attctcaaaa cctttatctg
    25981 ccaactcttc atatagtttc aatgcttggg tagttgctgg caatacaaga tccatttttt
    26041 ctgcttcttc taatgcaatc tttaaatctt tgataaaatg tttgacaaaa aaaccaggtg
    26101 tgtaatcttc ttttaaaata cgaggagaat aattgcttaa agaccagtta gctgcacttc
    26161 cacctgaaag cgtctcaatc actttcttta aatcaagtcc gtttttttgt gcatagacaa
    26221 gcatttccgt cattcctgtc atcgtacctg caatcatgat ctgattagcc atttttgtat
    26281 gctgaccttt cccagcagaa ccatgcaatg taaatgtcgt accaaattct ttgaataaag
    26341 gcagtacttg atcataaacg gcttcttctc caccgaccat gatcgtcagt gtaccattct
    26401 ttgctcctaa gtcaccaccg gataccggtg catctaacgc atctgcgcct ttttcttttg
    26461 ctgtttttgc aattttttct gccaggcttg gtgtacttgt agtcaaatca actaaaatat
    26521 gtcctgagat atctgcagaa aaaatccccg actgtccata gtagatttct tctacatctt
    26581 tggggtaacc taccattgta aagagaatat tactttgttc tgctaccgct tttggtgtat
    26641 ctgcccagat agctccttct gttactaaag gatcagcttt tgcttttgtc cgattataga
    26701 cagctacttc gtgtcctgct tctagtagat ggcgtgcgac agcacttccc atgacgcctg
    26761 ttccgataaa accaagtttc attttttcga cctcctaaaa atcaaaaatc cttgcttcta
    26821 tgctagtata cgctagaaac aaggattttt tcataaaaaa tgtttgaatt ttatctttta
    26881 aatgaattta attttatttt gattcgttca atcgagtctt cttcttcttt tcttcgcatc
    26941 tcttcttcat gataaacaac catgtcttga taaaattcga tcatagcttc gccgaaatgt
    27001 gtagatgaaa tatcataaag tttagcttct aaaacttttg gatctttagg aacttcactg
    27061 gaaatataat caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt tttccctaat
    27121 gaaggatgat cgaacaatcg gttcaaataa gcatttcctt ccacgacaca aggtgttccg
    27181 gctgccatcg cttctgtata agtaagccct tgtgtttctg aagtagaagc actgacaaaa
    27241 taatctgccg ctttataata aagtgctaca tcattgttgt ccacttctcc tgtgaactgc
    27301 acaaattctt ctaactgcag ttctgagatc aattcctcta actcttctac ataaggtcct
    27361 tttcctacga taactaatct ggcgttagga taagcggaga tgacctgagg aaacccatta
    27421 atcaaggctt ggatattttt ctcataagaa atcctgctta aagaaagaat catcaaatgg
    27481 ttttcctgaa gacctagtga ctcccttaat ttgctgatat cctgctgagt gatatcagaa
    27541 cgtttgaatt tttcaatgtc gataccagta ggaatcacgc gaagcggtgc tattacacca
    27601 tagctgcgta atgtatcaat aacacgttca cttggacaaa ctacacctgt cgtatgattt
    27661 gcaaataatc tagacaagta ttttacatgg gattgcctga tcacttttcc tttaccgata
    27721 taatgaaggt agtcttcata catcgtatgg taagtatgga tcagaggtat tttcaacttc
    27781 ttagctacca tcttcccaag tatccctgct ccgaattctg tatgggtatg aataagatct
    27841 agttctagtt ctttggcaac caaataagca taccacatgc ctctaacaac aactcttcga
    27901 tccttgaaag aaataaatgg tacacttggc attcgaatca catctttttc caacttatcc
    27961 gcattaggat cggtcgttgt aaaaatatag acttcatgcc catgtctttc taattcttgt
    28021 tttaatgttt tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt atcggtaaaa
    28081 aagccgattt tcatgttttt cgcctccaaa ctagtccttg acctgattta ctttttttaa
    28141 tttgctagag actttttcat tcagtacact ttcgtataca tttttaagtt cttcaccgat
    28201 tttattgata cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac tcggtagttc
    28261 ttgattggta atctgttgga tcagtcggat aaattcttcg tttgtcttcc ccatatagca
    28321 gttcttgcta tcttctaacc agccattata cactggaata tctcgaacaa gtacgttttg
    28381 tttgcttgct aaagcttcta aaacaacgat cccttctgtc tcttcataag aagggaaaaa
    28441 gaatagatct gcacctgaat acgcaccttc tatgatctct ccacgaatgt aaccaggaaa
    28501 caggacattt tcaggatgat cttcttttac aattttccgg atgttccgag gaatcgagta
    28561 catgggaaca tgaccaaacc aaataaatgt gtattcgggc atcttcttag caatttctac
    28621 gaaatcaatg attcctttac gttcgaaaaa caaaccgaca caaatgatta ctttttgatc
    28681 cggagacagt ttgaaatatt ctctgaactt ttgttctttc attggatcag ggtgatattt
    28741 ctctaagtcg atcccattgg aaatagcttg aatcggcaca cggataccat agctttccaa
    28801 caactgtttt gaataaggtg ttggcgtgat cagatagtcc gctttttggt acaatcctac
    28861 cagatatttt tttacgatag gagaaagctg gttcgatctg ataaaggaat ttcggaaatc
    28921 ttcctctgta gaatgcgcat gatagacaac tttttttcct tctcttctgg ctttattgac
    28981 catattatga ctattgattc cgtatgtatt gatgtgcagg atgtcataat cttctttttc
    29041 atctagtgta taagagatac cgacttccgt cagagcacgt ttttgatgat caagcgcacg
    29101 cccgattcca gattttgcaa gcatcttttc actttcaaaa tataataata ccttcacctt
    29161 acgtccccct tcacttatat agtataacat agcctacaaa ctgtcactca aacttaagcc
    29221 ctatttttgt ctattcttac tgacaaaaac gttttcttaa aaaagaacaa aaaaactggc
    29281 gtagctcttt tacaagccac accagctttt gatacgttgt ttattttaca tattcatgaa
    29341 caagttcaac gacttcttcc atcatatcgc attctttcaa tgcacgatca gcaagttcag
    29401 ccattttcgc tgtgtccaag cgtttcatta aactacgcgt tttaagaata gatgtcgcac
    29461 tcattgagaa ctcatctaga cccataccta ctagcaatgg aacagctgtt tgatctccag
    29521 ccatttctcc acacatacct gcccattttc cttcagcgtg tgctgcatca attacatttt
    29581 tgatcaaacg taaaattgat gggttatatg gttggtacaa gtaagaaacg cgttcgttca
    29641 tacggtctgc tgccattgtg tattgaatca aatcgttcgt acctacagag aagaaatcta
    29701 cttcttttgc aaacttgtct gctaagactg ctgctgctgg aatctcgatc atgatgccga
    29761 cttggatcgt atcagatact tccttgcctt cacttatcaa tttttgtttt tcttcttcaa
    29821 agatcgcttt tgctgcgcgg aattctttga gtgtagctac catagggaac atgatacgta
    29881 aattaccatg aacagatgca cgaagcaatg cacgcatttg tgtacggaac atttcgtcgc
    29941 cttgttcaga taaactgata cgcaatgcac gatatcctaa gaatggattc atttcatgag
    30001 gtaattgcag atatggtagt tctttatctc caccgatgtc cattgtacga acaactacag
    30061 gttttccttc cataccttct aatacagctt tgtaagcttc atattggtca tcttctgttg
    30121 ggaagtctgg agaatccata tacaggaatt cagtacggta cagaccaact gcttctccac
    30181 cattattatg gacaccaact agatcttttg gtgttccgat gtttgcagct aattcaaaat
    30241 gcttgccgtc agctgtgaca gtttctgcat tttttaattt ttcccattct gctttttgtg
    30301 ctgcgtaatc tgcacctgct ttttcaaatt cagctttttg ttcatctgta ggatcgatga
    30361 ttacatcgcc ttcgatcccg ttgacagcca aaatatcgcc ttctttgact ttagcagtaa
    30421 tttcttttgt cccaacgatt gctggaattt ctaaagaacg tgccatgata gcagaatgtg
    30481 aagtacgtcc accaatgtct gttacaaacg cttttacaaa attgcgatct aattgtgctg
    30541 tatcacttgg agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc attgatggat
    30601 ttggtaaagt aacgcctaat aagtgcgcta aaatacgttt agcaacgtca cgaatatctg
    30661 cagcacgctc ttgcatataa gcattatctt ccattgcttc gaacataccg atatacatat
    30721 ccgttacttc ttttagcgct gattcagcat ttacgctgtt gtctttaata ttttgcttga
    30781 tttgacctac catttctgga tctgaaagaa ccatcaaatg ggcatcaaat acttgcgctt
    30841 ctgcttcacc taagctttgc gctgcttttt cacgaatttg ctgcaattct tcagtagatt
    30901 tagctaatgc agcatccaaa cgagcttctt cagccgcagt atcttctact gttgccttac
    30961 tgaatgttaa atccggttga actagcaggt aagcttttgc aacggctaca ccgtcactag
    31021 cggcaatccc ttttagcatt tcaaccatta ttcagataag ccttctttct tcattgtttc
    31081 aacgattgct gccattgcat cagcttcgtc agcaccttct gcagtaatag taacatcaga
    31141 accttgacct acacctaaag acataacgcc catgattgat ttcaagttta ctgatttacc
    31201 tttatactct aagtttacat cagagttaaa tttgcttgct gtttgtacta atagtgtagc
    31261 tggacgagcg tggatcccag tttctgctac tacgtgaaat tctttctttt ccatattaat
    31321 cggtctcctt taagtgagtg ttgtttttat gtgagggttt tcttttttag taattcagag
    31381 aatcctaaca aatgccaccc tttcatctgt aaagattaac attttttttc gttagataca
    31441 acagttttca ttcaatttca gataaaagta ttcaaattat tccggattct ttttcatcct
    31501 tttcttcaca ttttataaca aacgtgttta tttccttttt cttgtcgctt tctcttctcc
    31561 tgacgtttat cattctttcc tttgaccctc tttgttttca tatgaaacga attacttgct
    31621 tcccttttta tttttagtat aataagagtc aaaggtcagg aatggtcaat agagatttca
    31681 ccttttctac ctcataaatc tcttaaaaaa ctaggggtta ttcttttacc tcatgaaaaa
    31741 cgagatgtaa aatatggatg tacccatttt tgtgaaagga cgtgaatgat tcatgctttg
    31801 tcaaaactgc ggcaagaatg aggcaacgat tcacttgtat gccactgtca atggtcagcg
    31861 gacacaactc gattactgtc agagctgtta tcaaaaaatc aaaaatcaac aaaatggagg
    31921 tctgactaat atggcccaaa atgatccatt tggatttggc agtttggacg atcttttccg
    31981 ttccatgtct cgccaaatgc aacagcaagg aaattatgag caaacaccac caacacaatt
    32041 tggcggaaac aacaacttca atggtggtca gccacctcaa ggcaatacag aaggtttatt
    32101 gggagaatac ggaatcaata tcacagaaca agcacgacaa ggtgatatcg atcctgtgat
    32161 cggacgcgat gacgaaatca aacgagtcat tgaaatttta aatcgccgaa ccaagaacaa
    32221 tcctgtcttg attggagaac caggtgttgg taaaacagca gttgttgaag gtttagcaca
    32281 aaaaatcgta gatggtgatg taccgcaaaa attaatggac aaagaagtca ttcgtttaga
    32341 tgtcgtatct ttagtccaag gtactggtat acgaggacaa tttgaagaac gtatgcaaaa
    32401 attgattgaa gagatccgtc aagcagaaaa tgtgatccta ttcatagatg aagttcacga
    32461 aatcgtcggt gctggttctg ctggtgatgg aaatatggat gctggtaata ttttaaaacc
    32521 agctttagct cgaggagaat tgcaaatggt tggtgctact accttaaatg agtaccgcat
    32581 catcgaaaaa gacgctgctt tagaacgtcg tatgcaacct gtacgggtag atgaaccaac
    32641 tgtcgaagaa acgatcagta ttttgaaagg tttgcaaaaa cgttatgaag actatcatca
    32701 tgtaaaatat actgatgagg ccatcgaagc agccgcaact ttatccaatc gttacgtcca
    32761 agatcgcttc ttgccggata aagccatcga tttgctagat gaaacaggtt ctaagaaaaa
    32821 cttgacgatc caaatcgttg atcctaaaac aatcgagaaa aaactgcaag aagcagaaga
    32881 acaaaaagtt ttagcttctc gagaagaaga tttcgaaaaa gcagcttatt atcgcgatca
    32941 gatcaataaa cttcaaaaaa tgaaagaacg tcagctcact gaagaggaaa cacctgtgat
    33001 taccgaaaaa gatatggaaa aaatcgttga acaacggaca ggtattccag tgggtgaatt
    33061 aaaagaaaaa gaacagacac aactgaaaaa cttagcagat gacttgaaag ctcatgtcat
    33121 cggacaagat aacgctgtag atcgagtagc gaaagcgatt cgccgaaacc gtgttggatt
    33181 aaacaaacag aatcgtccaa tcggctcctt cttatttgtt ggaccaactg gtgtaggtaa
    33241 aacagagctt gcgaaacaac ttgcctacga actattcggt tcacaagatt caatgattcg
    33301 tttcgatatg tctgaatata tggaaaaaca tagtgtctct aaattgattg gttctcctcc
    33361 aggttatgtc ggatacgaag aagctggtca attaacagaa aaagtacgga gaaatcctta
    33421 cagtcttgtc ttgttagatg aagtagaaaa agctcatcca gatgttttgc atatgttctt
    33481 gcaaatctta gatgatggtc gattgacaga tgcacaagga agaacggtca gctttaaaga
    33541 tacgatcatc attatgacaa gtaatgccgg aactggtaaa gtagaagcaa acgttggttt
    33601 tggtgcggca cgcgaaggtg ttactcgttc tgtattgaac caattaaaca actactttac
    33661 accagaattt ctgaaccgtt ttgacgggat catcgaattc agtgcattat caaaagaaaa
    33721 cttgatgaca atcgtcacac taatgctaga cgatgtcaac caaatgctgg cagcacagca
    33781 acttcacatc gaagttccta cgaatgtcaa agaaaaatta gttgacttag gctatgatcc
    33841 atctatgggt gctcgtcctc ttcgacgcac gatccaagaa caaatcgaag atggtatcgc
    33901 tgaattttat cttgatcacc cttctatcca tgagttgaaa gcaaaactgg ataaagatgg
    33961 aaaaatcatc gttacttcta aaccagaacg tttggctaaa gaatcagccg aagaaacggc
    34021 tgaataaatc cagttttata gcgtgagacg gtatttcttt gagaaatgcc gtctcattct
    34081 tttttcagaa aagtcaaaat actgttcatt ttataaaaaa gaaattataa taagagaaac
    34141 aagcaattaa aaatacataa aagaggtgag cgccatgaaa ttggtaaatg taacaaacag
    34201 ccattcccgt cttgtattaa accagcttga aaacactgat gcccatctag tcaaggtcta
    34261 cacagccgga aataccacta ttgtctacac tgaagcccct gaacataatg aaattctgct
    34321 gatcaatgac aaacgtaaaa tccagccgaa agaaatcgaa gatgctaaaa attatttctt
    34381 agataaaatc aaagacgccg tttaccacga ggatgaaatc aaagtaatcg aattggcagg
    34441 ccttgttgaa atctcgattc caaaacatga attaggcgct acattagccg tgtaaacaaa
    34501 ttttgaacat aataattaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
    34561 ctaaacacgt ataaacggcg aaagcagaag caactccttc ggaaataagc tgaaattcac
    34621 gaaaattgct tttcaaattt ttgtgaattc cagcttattt cttggagtta aacgcttctg
    34681 tctcagcctc tttttctttc tagctttttg atttttctct tttcttttgt acttttctct
    34741 tttcattttc agttctacgc gtgtctttat ttttaacttt ttcctcttta tggatgattg
    34801 atcgtacttt ttgtgcaatt tgctcgtttt tgtcccagtc ttttacatct aataatcgac
    34861 ataatcgtaa catctcttct tttttctgtt tttttctttc acgtctttgc cattctgttt
    34921 ccatgtgtca cactctcctt tctttaatca tatgtacgca aaataatttt tatatttttt
    34981 tgattagaca tttctatgat tttaatatag taaaaagagc cgtcacggaa aatccgtcac
    35041 ggctctaaac aaaatatgag aggctgtttt tgtttggcta ttcttattac ttgatgcagg
    35101 gcagcttgtg tcaagacttc tttacatcaa actttttaat tttacattgg gatatttatc
    35161 cgcaaaccag cgttctgcaa attggttctc gaataagaac aacggttggt cgaaacggtc
    35221 acgagccaag atattgcgac ttgaactcat cttctcatcc aaatcttctg gatcgatcca
    35281 gcgagcgatt ttgtgtccca ttggagacat gattacttcg gcgttatact catttaacat
    35341 acgatattgg aatacttcga attgtagctg accaacagct ccaatgatat attcttccgt
    35401 tagatatgtt ttataaagct gaatcgctcc ttcttgaact aattgataga ttcctttgtg
    35461 gaaggatttt tgtttcatga cattttttgc agtcactttc ataaacagct ctggagtaaa
    35521 tgaagggagt tcttcataag caacttttag ttttccttcg aacaatgtat ccccaatctg
    35581 ataattgcct gtatcatata caccgatgat atcgcctgct actgcttttt cgacgttttc
    35641 tctcgaatct gccataaatt gtgtgacatt gcttaatttc agttttttat ttgtacgttc
    35701 caaccagaca tccattcctc gttcgaatgt tccagaacag attcggacaa aagcgattcg
    35761 gtcacgatgg gctggattca tatttgcttg gatcttgaat acgaaacctg aaaattcttc
    35821 ctcataagga cttaccatct gatcatcttg tgttttgtgt ccataaggag ctggtgccaa
    35881 ttcaacgaaa gtctctaaaa aagtctgtac accgaagtta gtcaatgcag aaccaaaaaa
    35941 tactggtgtc tgatcgccac gagcaatttt ttcttgatta aatgcatctc cagcctcttt
    36001 gattagttct acttcttcta agacttgttc atataaggaa ttttcagtta gaggatgatt
    36061 ttcgggaagc agtccatctt catccaattc tactaatcgt tcatcctgat aattttccgg
    36121 acgataaaac tctacacgat tatggtagat atcataaaga ccttctagcc ctttacccat
    36181 accgattggc caattcatcg gataagattc gatatctaat agctcttcta attcttctaa
    36241 caattccaat ggttcacgac catcacgatc tagtttgttg ataaaagtaa atatcgggat
    36301 gccacgtttt tttacaactt ggaataattt ctttgtctgt gcttcgatcc cttttgcact
    36361 atcgattacc atgaccgcac tatccacggc catcaaagta cgataagtat cttcagaaaa
    36421 atcttcatgt cctggtgtat ccaagatatt tactcgtttc ccttgataat caaactgcat
    36481 aacagaacta gtaacagaaa tcccacgttg tttctcgatt tccatccagt ctgatttggc
    36541 aaaattacct gtttttttgc cttttacggt cccggcttga cggatagccc caccaaacaa
    36601 tagtaattgt tctgtaatcg ttgttttccc agcatccgga tgggaaatga tcgcaaaagt
    36661 tctgcgattg tccacttgtt gctttaattt tggattattc ataacctaac tccatttctt
    36721 ccattcaatt atttatattt tctctaggca cagattgtcc gatctcttaa agaaagacaa
    36781 tttcatatag ggctgtgaca aaagccgtgt agcagtctct ttttccgagt aaacggcgtt
    36841 ctagagacag cctatcagca ataagcccac ccaagccaaa aatacgaaaa gctgtttttg
    36901 cttggctgct cttattactt aatgcaggat agctttttca caacctttgt caactactac
    36961 aactgaatca gtatagcatg gattcagcga atgaaaaagc caccaaacca taagagtcaa
    37021 atattttttt gtcagctgac taaaagaaaa gacgtgttgc ttgtttattc aagcaacacg
    37081 tctttcataa ccaagtttcc aattatcttc ttggctcgtc atcttcgaat tcttttctga
    37141 agtatcttcg ttcttcttca ggttccgctt ctgtttcttc cggatcatga aaaatgacac
    37201 ggattttaag gattcgagaa ccttccattt cttcagaaac cagcgtaata ttatcgacat
    37261 caaacgataa cttttcgcct tcatccggaa tcatgcctaa tgctgtaatc aaataaccag
    37321 ccatcgtatc tacatcactc atatgcagat cactgtcaaa agcttcattg aactcatcaa
    37381 tcagcatacg tccttggatg atatattcgt gttcatcgat tttttcataa agattctcaa
    37441 cttcatctga ttcatcatcg atttctccga cgatttcttc taaaaggtct tctagtgttg
    37501 ctaacccaac gacgccgcca tattcatcta ataaaatagc catctgattc tgcgtctttt
    37561 tcatttcata taacagatca tcaataaaga ccgtttcagg aacgaataaa ggctcctgca
    37621 tgattttctt gatatctaaa ttatcaaagc cgaatttatg ggcagctttt agtaaattct
    37681 ttgtatgaag gattccgact actttatctt tgtcttcgtt ataaacaggg attcttgaat
    37741 agttttcgga taaaacttca ttcacatttt cttccacagt gtcgttgata tcaatcataa
    37801 atgcatccgt tcgtgggacc atgacttctc gggcaacttt cgtatcaaga gaaaaaaccc
    37861 cttggagcat ttctaattct tcattattca atacgccttc gttttcaagc atataacgca
    37921 tttcatctct ggtcattttt gaatcttcat catcaaaagt catcggcgtg atacgcgcca
    37981 ataaacttgt ggaagcagaa agcagccaga cgaagggctt agctactaca ccaatcaagc
    38041 gtatcagccc tgaagtgaac tttgcgactt cttctgattt atttaaagca atacgtttag
    38101 ggtacaattc tccaaaaaca atcgatacat aggtcaaaat agccagcaca atgatatttg
    38161 caatattttt tgccgctgtg ccgccaccta ataccggagc taaccgttcc gataaagtat
    38221 tagctagtga agcaccagac aaaatgttga ccaaagtgat tcctacttgg attgtcgata
    38281 ggaaattatt tggattctgc aatattttta aaagcttttg agatttcaca tctccttcat
    38341 cagctttttg ctcgattcga ttcttattga tcgaaacaac agcaatttcg gaagctgcta
    38401 aaaaagcatt gatcaatgtc aaaacgatca ataataaaat ctgcgctaac agcgactgac
    38461 tctcagggtc agcattcata atcgcattct ctccttataa aattaatttt tagtttacga
    38521 tagaaaaaaa cgtggcacta tagccacgtt aaaatggtat cacaaagcaa ttttctttgc
    38581 aataaaaaca agtgtactaa aactacctac gaggtcgtag caattttctt cttgttttca
    38641 ttttgcttgt cttctttgac gattttgatt atataggaaa tgatcgtctt gcagattgcg
    38701 taaaaaggaa cacctaagaa gataccaagc aatccggcaa tatttccagc tactagaaga
    38761 acgataatga tcgttaatgg atggattttc agtgatttcc caattacatt aggataaatg
    38821 atattcccat ccaactgttg gacgattagc acaaccacac aacaaagtaa tgcttttacc
    38881 ggatcattga agactgttac tagaaatgca ggtgctagtc ctagataagg tcctaaatat
    38941 ggaattaggt ttgtcaatcc cgcaatgacc ccaaataaga aagcataatc cacaccaatt
    39001 accatatatc ctaaaaatgt gaaggtacca acgaataaac attcgattgc ttgtccgcta
    39061 atgtaattag ccaatgtttt attcagttgc cccaataact ccacaatttg gtttcttctt
    39121 ttttctggga aaaactgttg gatgtttggc actagttttt ctccgtcttt caacatatag
    39181 aacaagatga atggtgctgt aatgattact acggttgccg aagcgatcgt tccaacgatc
    39241 gaacctaaac tattggatac tccactaagg aattgctgaa tgattgtccc gtaagaaatg
    39301 tctaattgct caaaatattt attgatatct acttccttga acaaaggcaa ttcggctacc
    39361 tcttttaacc agctttctac ctgtttgatg aaatcaggca tattagatgc caatgaggaa
    39421 atttgctgta ctagacttgg gatgacacta agtaaaatcc aaataattgc tgcaactaaa
    39481 aggagcagca caattgcgac agcccatatt cttttgattt ttgctttcat caacagcttg
    39541 acaataggat tcaataaata gtaaagaaag cctgcaatca gtacaggtgc aaataacgtg
    39601 gaaaaaaatg tcccgatcgg ttcgaaaata aagtttattt ctgatgaaat catgatcaat
    39661 gcagctatga tcaataattc aagcgaccaa aacattagtt tagactgtct aagtttttca
    39721 aacaatagat ttcctccttt ctttcttact agtatatatt atattcgtta aaaattcttt
    39781 tattcttttt ttatttttga aaacttgcca tactttttat ttgttttatt ttccagtata
    39841 ctatacgaaa ggaaggtggc tagaccatga aagttgtaca aacaaaagat acaatgagca
    39901 atatttactt agatgctgta cggatcaggc atcaagtttt tgtagtcgaa cagggtgtgc
    39961 ctttgtctcg agaaatagac aaagatgaag cccattgtat ccacttcgtc ctgtactcag
    40021 ataaaaaaga accgcaagga actgtacgtc ttttgccttt agaaaacgga aaaatgaaat
    40081 tacagcggat ggcgattctt tctgaataca gacaccaagg gttaggaaaa atattgattg
    40141 aagaagcaga aaactttgct aagaatcaag gctataatac gattttatta ggggcacagt
    40201 ccacagctga aactttttac gaaaaactag gatatacagc ttacggcgat ccttttgaag
    40261 atgctggcat gcctcatatt tatatgaaaa agactttata aaaatttatt tcgaaaaaag
    40321 caaaaaacag caagcttttg agaaaaatct ctcactggct tactgttctt ttttgtgttt
    40381 acttacgcga caattgcttg taacggttat accaaatatc aatatagcct tccgagaacg
    40441 ggccatctcc attattgatc cagtcaacca gaatcttcac attttctttt aggatgaagt
    40501 caatatcgtt cgggtaattc atcattttgc tgtgcatctc atattcatcg acatccaata
    40561 gtcgtttctc gccgtcagga aataccttga tatccaagtc atagtcgata tatttcaagg
    40621 cctcatcgtc cagcaaaaaa ggtgaagcta gattacaata ataggaaacc cccttttctc
    40681 tgatcattgc tattacattg aaccagtatt ttttatgaaa ataaacaatc gctggttcgc
    40741 gcgttaccca tcggcgtcca tcagattcag tgactaatgt gtgatcattc acgccgatca
    40801 tagagtgttc gcttgttttt aataccatgg tatctcgcca cgttcgatgc aatttgccgt
    40861 cgtgcttgta actttggatc gtcacaaact ctccttcttt tggaactccc atagcaaccc
    40921 ttccttaagc agcattttgt gctttgtgtt tgggtgttca ttacaccgac atcataatta
    40981 ttatatcaat aaaaatgagc tttgcctaga aaggattcct attgtttcga tttttctcta
    41041 actgatctac cagtttcatt tggaccttag gaaaaacgac cgaatcaaat gcatcaggct
    41101 tcaaccactt cgaagttttg ttctcggtaa attcttgttc tgctccctcg gttgcccgtc
    41161 cgtaaaaaag gagaacatgc catttcagat gactaaaaat atgagtaact tctcctaaat
    41221 gtcgtgtctg ccaaacaaag agttcctgct tttcaaatgg gagattttgc tcttcttctg
    41281 ctactaggtc atcaaacaga tcgatttctg gctttgtttc ccattctttt ttcaatcgct
    41341 catactcctc ttgtgtcact tccatcattg gaaatgtcca catattcgcc agcaattttt
    41401 ggctatctct ttcttcaaaa taatacgctc cagaatgatt ttgaagagct gcactgatat
    41461 aataaacgtc ttttggcttt gctttttttg tttttacagg aaaactcgtt tggattcctc
    41521 gtttgttagc taaacaaaag gcttggatcg gacaagtctc acactttgga gaagttggtg
    41581 tacagatagc tgaacctaga tccatcattg cttggttgaa ttcacctgga tgcttttcat
    41641 caatgatttt ccgcatcgct tcatcaaaga ttttcctgct agaagctttt gcgatgtctg
    41701 cttcaatgca aaacaatctg ctgactactc gcatcacgtt gccgtcaact gcaggttctg
    41761 gaagtccaaa tgcgatactt gcaatcgcgc ctgtcgtata tggtcctatt cccttcaatg
    41821 agctaatttc ttcaggcgtt tgcggcattt ccccatcaaa ttctgacatg atctgtttag
    41881 cagcagcttg gatgttccgg gctcttgagt aataaccaag gccctcccat gcttttaaaa
    41941 gcttttcttc tggagcattt gctagctctt caattgtagg aaaccattcc atgaaacgat
    42001 aaaaataatc gatcactgtg tctactctgg tttgctgaag catgatctca gagatccaga
    42061 tacgataagg atcacgatta tagcgccaag gcaggtttct tttttcttgt tcataccatt
    42121 gaatgaactg atcttgaaat tcttttgttt ctttatctgt ccatttttcc cattctttat
    42181 tcactttcct cttccttttc cgtaatgaat cgctggatca tatccaaatc aaatcctttt
    42241 tgatataagc tttgctttac ttttagattt ctttttttag gttcaaacct taaatttctt
    42301 cgccatagtt tatctccttg tttgactaaa taatcatatt ctatttcttg atctgcttct
    42361 tgcggaagat cagctatcgc ttgttggatc acttcttgag aaaagccttt cgtcatcaat
    42421 gtttgttgga tttttctcaa taattctttt tggctttttc cgtgattttt tccaaacgct
    42481 ttttcggctg tctgatgtgc attttgaact tggtcttcaa atgtatattg gctgagcgct
    42541 tgttcaatga tttcaggttt caaacctttt tgctgcatct gctgcgctaa tttctttggt
    42601 cctttgtcac ttaagcgcat attcgtgcgc acataacttt ccccatagac caaatcatcg
    42661 acaaggtcca attcttttaa gcgggctacg attttatttc gatcttcttg aaggatttcc
    42721 ttttctttta agtatgttcg gatttctttt tccgatcgca actgataact taagtaattc
    42781 attgctaggt gaaaaccgaa atcagctccg ctactcttct tgatctcttg gatagtttct
    42841 tcgtctaatt cccgtccttt taataatcga tagcgtacta atacatcttc agatacttgt
    42901 aaagcttctc cttcagataa atctacgcga tagaaagggc ctttgccttt actgattctc
    42961 gtaacggtta acataaacat cctcctcgaa catatattct ctatcatacc acagaaaatc
    43021 cccgtaatca ggtattatat gttagaataa accacagaaa aaagaaaaag ggagatactt
    43081 atggcagaaa caattgtcaa aactggacaa acggttcctt tgaaaatcaa gcgtttaggc
    43141 ataaatggtg aaggaatcgg ttatttcaaa cggttgatca tatttgttcc atatgcacta
    43201 ccaaaagaag aagtactagt gaaaatcacg aaagcaactc ctcgctatgc agaaggagaa
    43261 ttaataaaag tcaaaaaaac aagcaaagat cgcgtagtgg caccatgtcc tgtttactat
    43321 gaatgcggtg gctgtcaatt acaacactta gcttatcaag ctcaattgga ttttaaaaag
    43381 gatctgcttt tgcaagcatt ggaaaaattc aaaccagcag gctttcgatc ttatcaactt
    43441 ttgccgacga tcgggatgga tgagccttgg cattatagaa acaaagcaca atttcagtta
    43501 cgaaaaaata agcagactca aaaaattgaa gctggtttat acgaagcaaa ctctcatcag
    43561 ttagtcccta taacagactg cttggttcaa gaacctcgaa cacaaaaagt aatgaatact
    43621 gttgttcaac tattgaataa atttgatgca cccatctatg atgaacggac aaacagtgga
    43681 atcttccgca caatcatggt tagaatcggt gttaaaactg gggagttaca agttgttttt
    43741 attactcgca gtaaaaaatt cccgcaaaaa aacgcaatga tccgtgagat caatgaacga
    43801 ctgcccgaag ttgtttcaat catgcaaaat gtccaggcac aaaaaacgtc tctcgtgatg
    43861 ggggatgaaa cgatccactt atggggaaaa gaagccattg aagaacaaat caatgaagtg
    43921 actttcgatc tttctcctcg tgccttcttt caactcaatc ctcagcagac cgaattgctt
    43981 tatggcgaag gaatcaaagc attagatgtc cagcctaatg aaacgatcgt ggatgcttat
    44041 tgcggagttg ggacgatcgg cttaagtgta gcaaaacaag ccaaagaagt acggggaatg
    44101 gataccgttc ctcaagcaat cgctgatgca aaaaaaaatg cggaaagatt aggatatcac
    44161 aacactcgat atgaagtcgg gacagcagag caagttctgc ctaaatggtt gaatgaaggt
    44221 tttcagcctg atggtatcat cgtagatcca ccacgtacgg gtttggataa cgcattgatc
    44281 caatcattat tgaaataccc tccaaaaaaa cttgtttata tctcctgtaa tgtttctaca
    44341 cttgccagag atctggttaa tttaagtaaa aagttcaaga ttgaatattt acagtccgtc
    44401 gacatgtttc ctcagactgc acgttgtgaa gttgtcgtga agatgacgag actcgctgac
    44461 taaatgaaag aggttgtgaa aaagtctgcc ctgtatcaag taataagaac agccaaacaa
    44521 aaatagctct tcatgtttta tgaatactgt acttgtgacc gaagagttaa caggaggaca
    44581 ctatttattt aaaaaaaggg ttgtgacata gtttttgtca cagccctttt tgattattat
    44641 tggatattca cgtctatcac ttgataaaat gcatttgtcg tatcgctgat attccatact
    44701 gctaaaatca cattatatcc ttttcgatcc tgcggaagtg tgatcgtttg gttgactgtt
    44761 tttggtggaa cagttgcttt gtcgtctatt tttgtgatca attcaaaatc actaaatttc
    44821 aaaggttgat taggattcca agttggtttg gtaatgtagt aatcccaagt acttgtccga
    44881 tgttgtgctg ttaaattcca agtaatattt aatgttcctg gattaaccat gtttttatgc
    44941 catctgttag atgtctgttc atctaaaggc tcaaagccgc tgactcctgc actagcaatt
    45001 tttccatcaa tgaatgtatt tttggtagct tcgatacttt gcggttccca ttgtgctctt
    45061 ccgacatttt gatttaagtt tcctgctgct gatgttccta ggtatgcacg gcttcctgga
    45121 gaagtaatat aaccatgcgc aaaacctgga atgctaaaac cgaacaaccc taaactaact
    45181 aacccaataa acaataacgt actaatcttt ttcatgtaga aacctccttc atttgataac
    45241 cacattgtaa acgctttatt gtattaacac aaacattatg cttttataat tttatgttga
    45301 agtaaaaaag gtgaaaaaag taagatgcag attataacag aagtagacga gaaaagtaca
    45361 aatagaaaac atgagaatca aatcatatga tctttttcaa aaacatcatt catataaaca
    45421 aatgttcaac tttttaataa caacaattat caattttttt cttacttcac aaactaaata
    45481 caggtcttgc tgtgagcctt catggtaaac taatattata gaaaggagtt agtaaaatga
    45541 agatgataaa agatattcgt ttttgggttt gtgttatagg aattgtcata ttaggctttt
    45601 tatcagggtt gcttagcgga aatcccggaa aatattacta ttctttgcaa cttccaccat
    45661 ttgcaccacc tagctggata tttgggccga tgtggacctt actgtatata ttgatgggta
    45721 tttctcttta tttgttactt aatcacaaca acaaaaaaca gcgaaacaat cttgtcggtc
    45781 tttttgtgat tcagttcatt tttaatttta tatggtctgc tctatttttt aatttaagaa
    45841 atattttcat tgcagcaatc gatattacac ttttagttat tttcttgtca gtgcttatgt
    45901 atcagttgtg gctgcatcat cgcctggcaa tgtggctcat gattccttat tatttatggg
    45961 tactattcgc aactctattg aattattcaa tttatttttt aaactaagcg ctaaaaagag
    46021 acgtcctgta atttaacaca tagaaaaagt gccacctaag ttagattttt tgtctaattt
    46081 aggtggcact ctttattaac caaactattt ttttctagta cccacttgtt gttccatact
    46141 cattattctg gtcataacca taaccatttt gttcctgtcc agcgtaagga tcataacttt
    46201 gagtatcttg acttggtgta tagtactgac ttgtatcttc tgtttgctga taataggtat
    46261 tcggatcttg gtactgatca tatgtgtttt ctgaatagga agaataatcg gtgtaccctt
    46321 cactattcga tccatcaccg tttgtcgaat cgccatcttc cgaatcatca aagaacatca
    46381 ttgaagcatt ctcatttttc aaattaggta atgtatcgct tgtttttata tttaattgtt
    46441 tcttcagttc attttggatc gaaagaagat cattttttcc taaaatttga tagtagatat
    46501 cattgatcat ttgtccttcg ccttgcaact ggtcttgttt gattgtttca aacgccgggg
    46561 tgtagttcgt cgctacatca agcatgtcat cccaatctaa gtccgtcgtc acattgttct
    46621 caactgcttt taaaattttt ctataattgc ttacgccatc taaacttaaa actttattga
    46681 cgattttcgt cactacttcc cgctgacgac gttgtcgacc gatatcgcct tctggatctt
    46741 cgtgtcgcat tcgagaatag gctaaacctt tttctccatt caatgtttgt tcacctactg
    46801 gtacatggac accatccaaa gtaaagtcaa ttttattatt cactttgatg ccaccgaccg
    46861 cattgatcaa atcttccatg ccatccaaat tgatcgttac ataatgatca ataggaatat
    46921 cgagcagttg ctcaattgaa tccatggcca tttcgacacc accaaatgcg taggcatgat
    46981 ttaatttatc taccgttcca taacctacga tattcgtata aatatctcgg tccaaactga
    47041 tgatcgttga ttttttttgt ttcggattaa ctgttactac catcatcgta tcagaacgtc
    47101 cttgttccgt tcttcctaat ccaccggtat ctaaacctaa tagcaaaacc gaaaaaggtt
    47161 ctttgtcatc aatgcttact ctctcagaac gtttggagga tattcgatta gagtttttag
    47221 aaattttgtt tactgtttgg ctcgcttctc ccataaatct gattccgtat gctgataatc
    47281 cggcaatcag caaaacgaat acccctaaca aggttaatag aactttttgc catcgcttca
    47341 taatacacct ctgtttcaca ttttctaaaa tattgtctca tataactgtt caatcagcaa
    47401 attcactttt taatcataag aaaactttaa aaaacatttt tgtgtaacga ttattatttt
    47461 ttaaaatttc tcaaaattct taactatcat accataccgt gaaaaagatc gaaagaccga
    47521 atacacgcag ttcgcaatcg ttttatcttt ttttatgtat tttttccaga aaaagcgaac
    47581 aacaaggtat tttccaaata atcgtgcagt aaaatgatcg cacatacctt tcccatcttt
    47641 ttaacagtct ttgcctaccg ttcagattat cattcgattt cacgacaatt tcttcctatg
    47701 actggtcctt ttgttaaacc catcttctat tcgttttatt tccagctcac tgcatggctc
    47761 ttaaaatgac tcgaactagt gattgcatta ccatcatctt ttaatggata gatatgctcc
    47821 tcttcaaagt aaaacgattt tcttttaaag taatagaatt gacatggtaa tgagtatgta
    47881 tactagtggt ttcacaaatg gtcttttatt caacatccaa aaactgaaat ttctttaaaa
    47941 tccattacta acgaagaaaa tgttccttgc ttgataatta gttaggacat gtcaagtgaa
    48001 caaatgcatt tacttagcca aaaaatctaa aaacttcata aatagacaaa aagacacgac
    48061 tctctaagaa gtcgtgtctt tattcaacaa attattataa tttagtcact tcagcagctt
    48121 gaggtccgcg tgcaccttca acaatcgtga attctacttc ttgaccttct tctaggctct
    48181 tgaagccgtc tccttgaatt gctgagaaat gaacaaatac gtcatctccg ccgtctactg
    48241 taataaaccc aaatcctttt tcattgttga accatttaac cgttccgtgt tccataaatg
    48301 tatatcctcc aaaaaagttc ttgcaagtaa ccttgcaaga acattatacc caatttagaa
    48361 aacgctagca agctttcgct cttttatttt ccaaattatt tttaagactg atatgacgga
    48421 ggaaatggtt attttgctgc ttcgaaacgt ttgtttactt catcccaatt aataacattc
    48481 cagaatgctg caatataatc tggtcggaca tttttatatt tcaaataata agcatgttcc
    48541 caaacgtcca aacctagaat tggtgtcttt ccttccatca atggagaatc ttgatttgca
    48601 gtagaggtaa tagctaattt tccattttcc attacaagcc aagcccatcc agaaccaaat
    48661 cgtccagcgg ctgctttttt gaattcttct ttaaaagaag aaaaatcacc aaaagcttca
    48721 ttaatcgctt cttttatttc tcctgtaggt tcgccaccag catttggtgc catgatttcc
    48781 cagaaaaatg aatggttagc atgtccgcca ccattgttac gtacagctgt cttgatatct
    48841 gttggaatag cgtccatatc agataataat tcttctattg ttttttcgcc taattctggg
    48901 tatttctcaa ttgctgaatt taaattcgtc acataagtat tgtgatgctt atcatgatgc
    48961 agatgcatcg tttcttcgtc aatataaggt tccaatgcgt catatgcata aggtagatct
    49021 ggtaatgtgt aagtcataaa aaaattcctc ctcaatgttt tcttaataat gtctacatta
    49081 caaagagtaa caaagagaag gaaattcttc aattgttatg cctagtcgtc tttttcactt
    49141 tgacctactt cttcttcaaa aacctcagtt tctttaaaaa taaacaactt gctgaaaata
    49201 tagttcgcaa ggataacaat aatttgagtg atgattttcg ccacatactc attgctgttc
    49261 ataacattga tcaacaatac cattgccccc atgtccaatc cgtaagaaag gatacggtag
    49321 aagaagaact taccgcattc aataagtgca tgtgtaaaat tcggtgattt tgaatggaat
    49381 acccaaagtt tgttcgttac aaaagcaaac aatacagaac aaaaccacga aatagaattg
    49441 gataaggcca tacttaaatc aaaatattgg cgtgcgagag caaaactaac aaaatttaca
    49501 atcgtagcca aaccaccaaa aaagagatag actagtatct cccaatatcc ttttgcttct
    49561 aaatatcctt ttaactgtcg atactgcttc atgagccaac ctaccttatt aaaaattctt
    49621 atttatcata ctctatattt caataacttt catctttatt taaaaaaatt aatttttttt
    49681 gatcctttgc ctttgtctct gcttggatcg tcacatgtgt aataccatat tttaatttta
    49741 ataaagaaat tatacgagga tatatctttt caatctcgct tagtagctga tcttcaaaat
    49801 caatatgtcc gtcgaaaata attatctttt cgctatactg ccaaatatgg acatgatgaa
    49861 catcctttac cccatcgatt gcttcaattt cttgttccat tttttcatag tcgatagcag
    49921 gtgcactttg tatcaaaata gcggcagctt ttttgatcac cattatcgct tctatcaata
    49981 tatatatcga aatgatcagt gtaataactg gatcgatcca gtagatatcg aaaaaacgga
    50041 tcaaaattgc tccgataatc acacttaccg aagacagtgt atcacttaac aaatgcaaat
    50101 aactagattt tatattcaaa ctttcatctg cttcttgtcg taataataat actgagaaca
    50161 aattcgcgag caatccaata actgctactg ttagcatcaa atgactattt atcttttctg
    50221 gtgaagtgaa ccgcttgaat gcttcaacgg ttaaaaacaa ggataatatg atcaatatga
    50281 atgagttgat gaaagcactc aatatttcta aacgctggta gccatacgta cgttttttat
    50341 ttgcttcttt tcgggccatt ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac
    50401 taaggttatg gaaagcatct gaaacaagag acaaactccc tgacataatc cctccaataa
    50461 attctgataa agtgatcacc gcattcaaaa ccactactcc aaatagtttt tttgatgatc
    50521 tctgtggctg tgtaggcact attccttcct ccattttatt tatattggat agtctttaga
    50581 caagaataac atttttttga tatttcacca atcattcagc tgtttcctta aagcacttgt
    50641 atttcacttg atacttcgct agaattgcaa gtgatgacac tactagatga ggagtactta
    50701 tgacaacaaa acaattaatt atcagttcct ttatacgatt caaagaccta aaggaggcga
    50761 gaaatgtccc attttggaaa gccattgtct atcttttagt tttgagtata attatggctt
    50821 tgccaatctc ttttcaagtt tttcaagtct taaacaacat tcaattggat gggcaaaaga
    50881 ttgctactcg cattcctgat tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag
    50941 aaggattcat ttaccaaaca aactccatta tcttcacatt tgatccagag ggaaaacgaa
    51001 cagaacagga catctctact gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa
    51061 acaaacttat catagctctt cctaatacag gcacgacttc tgctttgttg aacaataacc
    51121 aattagaact tccctacacc aatgaatctt taaaaaactt aacaggtaag cagctccgtt
    51181 cttttttaag tgaagcatct ataccacttt ggataaaagt cctgacattt atattttcta
    51241 tttatcctag ttttctgaat ctgctgatca ctttgctatt taccaacatt gctgcttttc
    51301 tctatgcacg ctttcgacta acaaaagcta cttttcttga ttgtttaaaa acattgatct
    51361 attcaatttc attgcctact ataattgcaa caatcttgat ggtctttctt ccttcttttg
    51421 acactagcgc cttcattgct tttgcaggaa tctttatttt tgctcaagct gtcaaaggat
    51481 gggcaaaaat atcgatcaat taagaaataa agacaactcg ttatttctac ttcaatagac
    51541 ccttttagag gttgtgacat ttttttgtca cagccccttt tttatttgaa aaatatttct
    51601 ttctcaaaaa acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa
    51661 agaaaggcgg gaaattaact atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg
    51721 atgaatttat cgaagaaatc catactatag cgcctggtta tatctttaaa acagaattac
    51781 aaccagagga tctacaaaat gtagagatca gtttgggctg gaactcagac taccaagaaa
    51841 aactattagc ctcttctacc ttgaaatggg tccaatctat ctcagctggt gttgatcagc
    51901 taccccttga gactttttca aagaaacata ttttactttc aaatggtagt ggcatccaca
    51961 gtgaatcgat tacggagcat gtcttaggaa tcatccttgg gtattctcgt ggtctttttc
    52021 aagcacaaaa ggcacagagt gacatgaaat ggttaggttc atcggtccat taccaatcta
    52081 tccaagggaa aaaaatacta atcattggaa ctggccatat tggaaaaatg attgcaaaaa
    52141 aatgcaaagc attagacatg aactgctatg gaatcaatac atccggtcat ccagcagaag
    52201 gaatggaaaa gactttttct ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg
    52261 tagtcaatat cttacctcta acggaggaaa caactgggct ctttgatcag catatgtttg
    52321 aacactttga tccaaaatct gtattcatca atgttggccg tggtgcttct gttaagacac
    52381 aagatctggt gcaggcttta aataaccagc aactttcctt tgcagcatta gacgtttttg
    52441 aggaagagcc tcttccacaa gatcatcctc tttggaaaat ggacaatgta ctgattactt
    52501 cccacattgc aggtttgaca cctgattttc aaaagaaatt aatggctatt tttttaacca
    52561 atctgaaaag ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag
    52621 gatattaata gctgaaaaca atattttcaa aagaaaaaag agaaggtgac aaaaattgtg
    52681 tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca ggttgggatc
    52741 ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct cgataaaaaa
    52801 aagtgtccca tcaatttcca ctttctttac aggatgttct ggctgttcaa ctgacattcc
    52861 aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa aacgtattcc
    52921 gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt
    52981 taatttcatc ccaacaacct actttctctt ttctttctat tgattcatct tatcgtctag
    53041 aatatccaca tcgcctgtct ctccaataac cagtagatta tcattttcac gaacatattc
    53101 atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga ctactgttaa
    53161 gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt taggattgga
    53221 gactcggatt tctgctaaag aatatttatc tgacaactct aggtaatcta aaatattctt
    53281 tgacactaaa ttatgcgcaa tccttattcc catgtctcgc tcgggatgga cgacgcgatc
    53341 agctccgatc ttttcaagta ctctggcatg atactcattt tgggcttttg ctaaaatatt
    53401 tggtacgccc atttccttta ccatcagagt aactaagata ctggcttgaa tatcttctcc
    53461 aatagcgaca attacatgat cgaaatttct tatgcctaat gatcgaagtg tcatttcatc
    53521 ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga cccgatcttc
    53581 attactatca atagccagta cttcttgacc agcttcgatc agtgttcggc agatacttcc
    53641 gccaaatcgt ccaagaccta caattgcaaa attttgtttc accgttttcc tcctttagta
    53701 tcttcttttc tcagtataac aaagccttta caaaaacaaa agcaagcatc attattgtaa
    53761 aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt tatcatcgtt
    53821 tttatttcac caaaccatgt ttgaatgcat agatagcggc ttgtgtacgg tcttccactt
    53881 ctagcttagc caatatattg gaaacatgtg tttttactgt tttcagtgta ataaacaatt
    53941 cgtccgctat ttcctgatta ctttttcctt cagaaatcag cattaaaatt tcattttccc
    54001 gattcgttag ttcttcatgt aatacaggct catttcgccg actcattttt tccatcattt
    54061 ttgttgtcac ttctggttct aatactcgct cgccacgttg cgttgccctt atagcatcag
    54121 caatttcatg tgcggtagag gtcttcaaca aataccctgc tgcacctgct tcaatggcag
    54181 gataaacttt ttcgtcatca ataaaactgg ttacgataat gattttagct tctggccaat
    54241 cttttaagat tgcttttgtt gattcgatac catccatttc ttccatgact aagtccatta
    54301 aaatgacgtc cggtcgaagt gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt
    54361 ctccaattac ttcaatgtct tcttgaatag ataaataaaa agagacgcct aagcgcacca
    54421 tttcatggtc atctactaat aaaactttta tcatccttat ctatgcctcc ttcatcaaag
    54481 ggaccttgat ttctacactt gtaccttgac ctttaaaact aatgattttt actgttccac
    54541 caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt ttcgtttcat
    54601 tcatattaaa ccctgttccg tcgtcaataa tacgtaacaa aagattattg tctattttgt
    54661 gcaaatatac ctctaattca ttggctttag catgtcttaa tgtatttgat aacaattctt
    54721 gaacgattcg gaacaagtga tcctcaatgg aactagatag ttttacatct tctacatccc
    54781 atttcagcga aatctggatt ttattttgca attccttcaa taactgctcg ataccttgct
    54841 ttaaactttt ctcttctaaa ttgactggac gtagatgaag aagcagcgca cgcatctcag
    54901 actgggaagc gttgatgatc tctgctacca ttttcaactg cttgcgaaac atttctggtg
    54961 tctcgctttt ttctgcttgc tccgtcaatg ccgacatcat catcattgct gcaaacaatt
    55021 gttgcgaaac tgaatcatgc agctcacggg ctaatcgatg tctttctaat tctaaaattt
    55081 cttctttggt ttgtccatct acatattggg gacggctcgt tacaatttgt aattcactag
    55141 atacttcaat cattttttct tttatcttag tgatttcctt atcaatatct ttgatgtata
    55201 aatcgtccga tgcacttggg atcggaagaa gcaacaattt ggattcataa ttgccagaag
    55261 aaagtgcacg cagcttttct tcgattttcc cgtattttgt cttttgaata agtgataata
    55321 atagaaagac aattaatcct acacctaagg aaatggctaa caaatggaag ataagcggaa
    55381 catataataa acgtgcacgc agtaattctc cccaccaatg actttgattg ctggcataaa
    55441 aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa taaacagcta
    55501 gcatcgctct ggatattttt cccatcatat tcgtatcacc tcgacatctc caatcaaggt
    55561 attagtaata atcttcaatc tacgagggtt ttcatcataa tcactgctgt agattttgat
    55621 ttgttcattt ttcaaagaaa attgctcttc ttcaaacaag acatttccta ctaatgtcgc
    55681 atgctctaat cggatggcta cgcccaaagg tactaagatc cgtgttctgc ctatcccttt
    55741 tcttacaatc acaatattat catcttttgg caaaagcgta ttgcccaaat ctatgatagt
    55801 atcaccagaa atgacggcga tgttgatatc gtcccattca tagacgtcgt ttccaatacg
    55861 ttggttaccg aataattgct gtcttttccg ctcattatta tgtgttttta attgctctgt
    55921 ttggatcatc atgatctgtt tctttcgcca aaaagcattt ttggttaaat ctatccctga
    55981 aatctctaca cctttcagac caataaataa aacggcaaat acagccatca tccataaagc
    56041 aggactgttg actaagctaa aaaaaatgac caaacttcct aatatcaatt gaaaattttg
    56101 aaatttagta cgaggatgtt tgcgcatagc tagatatata ttgaaaatac cgaatatcac
    56161 taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc
    56221 ttcaactacc acaaaaaaac gccaagaact attcatcgct ctttctccct cctgacacat
    56281 tctcatttca tcatacctat ttttttaact gttggctata ggactttaga tgtaagatcg
    56341 tttgttgaaa tctaaaatac acaaagagaa aaagatctag agtgtatcat cagtaagatg
    56401 aactctctag atctttcagt cagactacgc ttgttctact ttgacgattt taactaacat
    56461 atcgccacct ggagtagcaa tagttacttc atcattttta cgtttgccaa tcaatgcttg
    56521 cgcaatagga gaatcattgg aaattttgcc agaaaacgga tctgcttcag cgcttccgac
    56581 aatcgtgtac tcttcttctt ctccatctgg tagttcgatg aatgtaactg ttttaccaat
    56641 tgatacctca tctgcatcta caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt
    56701 ttcaattgtt gtgattcttc cttcaacaaa tgcttgttca tctttcgctg attcatattc
    56761 agagttttcg gaaagatctc cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc
    56821 gcgtttgact gtttttagtt cttctaattc tttttctaat ttttctttac cttctagagt
    56881 cattggataa actttctcta ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa
    56941 aggtaatctt ttgaggaata tagtccaaaa aagattacca cgcttttctt caaatgtaaa
    57001 tgtttttcta aaaatgatca atgtctagag cctgtttttc taagaattat ttacatattt
    57061 ttccactaat tccatatgtt cttcataagt tttagcgaaa taaacatttc cagtgctcgt
    57121 atctgctacg aaataataat agtcattttc tgctggttct aatactgcct tgattgcctc
    57181 ttcgcttgga ttgtcaaaag gtccaggtcc atagccggta ttggtataca aattatatgg
    57241 tgaatctaca gctgtatctt catacgtcac taattctttg tgttcaccta atgcgtataa
    57301 aacagagata tctgactgaa gtggcatatt tgctttgatt cggttaaaga agacttgtgc
    57361 aatgttttta cgatcatttt cttttacgcc ttctttttca actagagaag ctagagttaa
    57421 aacttcttgt acagtcaaat ttttctgctt gatagctgaa taatagtttt gcatgaccgt
    57481 attcgtcttg ttcaccattt caatcactag atccttaagc gaagtactcg tgtaataatc
    57541 atatgtggct gggaataagt aaccttccaa acggtatttt gtgttctcag ctttagatgc
    57601 actttccaac agttctggga atttttggtg caactcgtta aagaaagtct catcattcac
    57661 taaatctaaa aattctttct tgtctttacc agttaccttc gctacccgtt cagcaatctg
    57721 atcgatatcg tacccttctg gaatggcaat tttcccatct gcaacttttg ttggctcaga
    57781 agtaccgcct tcttgcaact gtttgccaat ctcgtcaaga gtcatattag gcgctaactg
    57841 ataatagcca gcttgaaagc cagttaaatt tttaaatttt gtataataat tgaaaacaat
    57901 cccgctttta ataatattgt ccttctccaa aatctcacca atctgcttgt tagaagagcc
    57961 actcgggatc tcgacttgga ctaattggtc atctgattta tctaaaggct tcaacccgct
    58021 atctacataa cgatacacgg taaatccaag aaatccgcca ataatcaata atgtaagagc
    58081 aatcactaaa atgatcttcc tcacaatctt atcttctttt ttacgcagat ttctccgctt
    58141 gtcgattata tctgctgtag ctgccttact ggcttctttt ttatctttac ttgggtgttg
    58201 tttgtttcct gacaaaacaa ctcctcctta ctatcaagtt cgtaacttta ttcattatac
    58261 atgatatttg aattggcgtg aatgatttta tttaaatatt ctatcaaaaa tgtaagtagt
    58321 aaatccatta ctataagatt aaaataaatg ttatcaagat acaagctatt ttagctttat
    58381 tct
//
LOCUS       DACDHL010000010        54454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDHL010000010 DACDHL010000000
VERSION     DACDHL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.010"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="IV228_000634"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="IV228_000634"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8716573.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="IV228_000635"
     CDS             complement(1036..1956)
                     /locus_tag="IV228_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8716574.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /gene="xth"
                     /locus_tag="IV228_000636"
     CDS             2069..2821
                     /gene="xth"
                     /locus_tag="IV228_000636"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8716575.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="IV228_000637"
     CDS             3685..4218
                     /locus_tag="IV228_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8716576.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4708..5232)
                     /locus_tag="IV228_000638"
     CDS             complement(4708..5232)
                     /locus_tag="IV228_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716577.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="IV228_000639"
     CDS             complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="IV228_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8716578.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5821..6801)
                     /gene="pta"
                     /locus_tag="IV228_000640"
     CDS             complement(5821..6801)
                     /gene="pta"
                     /locus_tag="IV228_000640"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8716579.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6954..7634)
                     /locus_tag="IV228_000641"
     CDS             complement(6954..7634)
                     /locus_tag="IV228_000641"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8716580.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7760..8629
                     /locus_tag="IV228_000642"
     CDS             7760..8629
                     /locus_tag="IV228_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8716581.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8655..9170
                     /locus_tag="IV228_000643"
     CDS             8655..9170
                     /locus_tag="IV228_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716582.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9217..9780)
                     /locus_tag="IV228_000644"
     CDS             complement(9217..9780)
                     /locus_tag="IV228_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716583.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9835..10014)
                     /locus_tag="IV228_000645"
     CDS             complement(9835..10014)
                     /locus_tag="IV228_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716584.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10091..10600)
                     /locus_tag="IV228_000646"
     CDS             complement(10091..10600)
                     /locus_tag="IV228_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716585.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10696..11076)
                     /locus_tag="IV228_000647"
     CDS             complement(10696..11076)
                     /locus_tag="IV228_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8716586.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11406..12155
                     /locus_tag="IV228_000648"
     CDS             11406..12155
                     /locus_tag="IV228_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8716587.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12476..13693)
                     /locus_tag="IV228_000649"
     CDS             complement(12476..13693)
                     /locus_tag="IV228_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8716588.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(13951..15228)
                     /locus_tag="IV228_000650"
     CDS             complement(13951..15228)
                     /locus_tag="IV228_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716589.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15307..15951)
                     /gene="pcp"
                     /locus_tag="IV228_000651"
     CDS             complement(15307..15951)
                     /gene="pcp"
                     /locus_tag="IV228_000651"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8716590.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16150..17094)
                     /locus_tag="IV228_000652"
     CDS             complement(16150..17094)
                     /locus_tag="IV228_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8716591.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17096..17782)
                     /locus_tag="IV228_000653"
     CDS             complement(17096..17782)
                     /locus_tag="IV228_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8716592.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18256..18576
                     /locus_tag="IV228_000654"
     CDS             18256..18576
                     /locus_tag="IV228_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8716593.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18730..19635
                     /locus_tag="IV228_000655"
     CDS             18730..19635
                     /locus_tag="IV228_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8716594.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19632..20405
                     /locus_tag="IV228_000656"
     CDS             19632..20405
                     /locus_tag="IV228_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8716595.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20586..22010)
                     /locus_tag="IV228_000657"
     CDS             complement(20586..22010)
                     /locus_tag="IV228_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8716596.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22221..22667
                     /gene="nrdI"
                     /locus_tag="IV228_000658"
     CDS             22221..22667
                     /gene="nrdI"
                     /locus_tag="IV228_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8716597.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22732..24714)
                     /locus_tag="IV228_000659"
     CDS             complement(22732..24714)
                     /locus_tag="IV228_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8716598.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24728..25624)
                     /gene="murQ"
                     /locus_tag="IV228_000660"
     CDS             complement(24728..25624)
                     /gene="murQ"
                     /locus_tag="IV228_000660"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8716599.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25627..26688)
                     /locus_tag="IV228_000661"
     CDS             complement(25627..26688)
                     /locus_tag="IV228_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8716600.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(26899..27426)
                     /locus_tag="IV228_000662"
     CDS             complement(26899..27426)
                     /locus_tag="IV228_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8716601.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(27493..28995)
                     /locus_tag="IV228_000663"
     CDS             complement(27493..28995)
                     /locus_tag="IV228_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8716602.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29253..30374)
                     /locus_tag="IV228_000664"
     CDS             complement(29253..30374)
                     /locus_tag="IV228_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8716603.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30500..31441
                     /locus_tag="IV228_000665"
     CDS             30500..31441
                     /locus_tag="IV228_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8716604.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31591..32487)
                     /locus_tag="IV228_000666"
     CDS             complement(31591..32487)
                     /locus_tag="IV228_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8716605.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            32756..34096
                     /locus_tag="IV228_000667"
     CDS             32756..34096
                     /locus_tag="IV228_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8716606.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            34314..34757
                     /locus_tag="IV228_000668"
     CDS             34314..34757
                     /locus_tag="IV228_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8716607.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(35261..36679)
                     /gene="nhaC"
                     /locus_tag="IV228_000669"
     CDS             complement(35261..36679)
                     /gene="nhaC"
                     /locus_tag="IV228_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8716608.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(37082..38305)
                     /locus_tag="IV228_000670"
     CDS             complement(37082..38305)
                     /locus_tag="IV228_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8716609.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(38336..39175)
                     /locus_tag="IV228_000671"
     CDS             complement(38336..39175)
                     /locus_tag="IV228_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8716610.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(39192..39872)
                     /locus_tag="IV228_000672"
     CDS             complement(39192..39872)
                     /locus_tag="IV228_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716611.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(39874..40947)
                     /locus_tag="IV228_000673"
     CDS             complement(39874..40947)
                     /locus_tag="IV228_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8716612.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(41594..42265)
                     /locus_tag="IV228_000674"
     CDS             complement(41594..42265)
                     /locus_tag="IV228_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8716613.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(42352..45030)
                     /gene="mgtA"
                     /locus_tag="IV228_000675"
     CDS             complement(42352..45030)
                     /gene="mgtA"
                     /locus_tag="IV228_000675"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8716614.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            45689..46126
                     /gene="ndk"
                     /locus_tag="IV228_000676"
     CDS             45689..46126
                     /gene="ndk"
                     /locus_tag="IV228_000676"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8716615.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(46148..47014)
                     /locus_tag="IV228_000677"
     CDS             complement(46148..47014)
                     /locus_tag="IV228_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8716616.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(47174..49267)
                     /locus_tag="IV228_000678"
     CDS             complement(47174..49267)
                     /locus_tag="IV228_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8716617.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(50016..50639)
                     /locus_tag="IV228_000679"
     CDS             complement(50016..50639)
                     /locus_tag="IV228_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716618.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(50798..52195)
                     /locus_tag="IV228_000680"
     CDS             complement(50798..52195)
                     /locus_tag="IV228_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8716619.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(52227..53198)
                     /locus_tag="IV228_000681"
     CDS             complement(52227..53198)
                     /locus_tag="IV228_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8716620.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            53572..53997
                     /locus_tag="IV228_000682"
     CDS             53572..53997
                     /locus_tag="IV228_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716621.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        15719 a        11284 c         9619 g        17832 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa
     4501 tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc
     4561 gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga
     4621 ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga
     4681 cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc
     4741 taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc
     4801 catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg
     4861 aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag
     4921 tcccattccc caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg
     4981 aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag
     5041 cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg
     5101 tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc
     5161 catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac
     5221 ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt
     5281 gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta
     5341 aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa
     5401 cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta
     5461 cccgataaat atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg
     5521 tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca
     5581 acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg
     5641 aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt
     5701 ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa
     5761 agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg
     5821 ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt
     5881 tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc
     5941 ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg
     6001 aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac
     6061 tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag
     6121 ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc
     6181 ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt
     6241 tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca
     6301 atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt
     6361 ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt
     6421 cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc
     6481 aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct
     6541 acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat
     6601 cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt
     6661 ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat
     6721 acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct
     6781 taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca
     6841 ctattccaga aaaaaagaac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat
     6901 taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact
     6961 gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt
     7021 ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga
     7081 gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt
     7141 ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct
     7201 gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga
     7261 acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca
     7321 ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc
     7381 tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga
     7441 tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag
     7501 atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt
     7561 agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga
     7621 atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc
     7681 acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca
     7741 gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt
     7801 agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc
     7861 cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga
     7921 tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga
     7981 aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat
     8041 tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa
     8101 tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac
     8161 gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt
     8221 ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt
     8281 tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga
     8341 tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa
     8401 aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac
     8461 gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat
     8521 gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga
     8581 atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga
     8641 aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc
     8701 ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa
     8761 caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc
     8821 agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa
     8881 ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga
     8941 cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca
     9001 tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg
     9061 atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta
     9121 aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac
     9181 ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc
     9241 ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa
     9301 ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt
     9361 cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc
     9421 ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa
     9481 caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat
     9541 gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc
     9601 ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa
     9661 aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag
     9721 aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat
     9781 agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt
     9841 taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc
     9901 agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt
     9961 gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc
    10021 tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa
    10081 tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga
    10141 ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt
    10201 tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt
    10261 tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc
    10321 cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg
    10381 cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt
    10441 cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt
    10501 cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta
    10561 ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt
    10621 ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg
    10681 acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa
    10741 cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga
    10801 ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc
    10861 acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc
    10921 agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct
    10981 gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac
    11041 aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta
    11101 agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc
    11161 tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac
    11221 tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca
    11281 gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc
    11341 atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct
    11401 catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa
    11461 attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca
    11521 tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat
    11581 ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta
    11641 ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga
    11701 tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa
    11761 tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc
    11821 ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca
    11881 ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat
    11941 attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc
    12001 atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc
    12061 ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg
    12121 aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta
    12181 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct
    12241 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    12301 ttcttggagt taaacgcttc tgtcccagcc tctatttcct acgattagtc aagtaaaagg
    12361 tgcaagaaaa atagctcgta aacgaaagcg acaaaatcaa agcacaccaa ataaagcccc
    12421 gttcgtaaaa aattttcgat ctgggcaaag aagtggtata ctgtaagtgg tttagttaat
    12481 ggtccactga gaccgcgtac gcatagtcgt actctgtatg tgccctaacc atagcgtgca
    12541 tacatttcag aagcttgttc atacaagcaa ctgtggcaac cttatctttc ttagggatag
    12601 gttgcttttt taatttgtag taataatcga caatatgatt aggagctgcg cgttgctggc
    12661 gaatcatatt tcgaatggtg aagaaaataa ttttccgtgc tttgggatta ccacgcttat
    12721 tgatgcggtc ttggccagta tacttacctg aatgataccg tcggatgtcg attccgacaa
    12781 aggcattcaa ttgtttatgg gttttaaatc gtgtgatatc tcctaattca ccgagcaata
    12841 aagcggctgt gagtgcgcca atcccaggga acgtttggta caacaaaaag caaggagagc
    12901 cttcggcttt tttgatcatt tgtgtcgcca gtgctttttt ttgttcgagt aaatcttgga
    12961 gaagttcggc gtaatagatc gttttttgac agtggatact gtctttttca acagctggat
    13021 aggcacactg tgcatagtgg atgatttgat ctgccttttg ctcggcacga ttcgcggaga
    13081 tttttttccg tgtttcgttg atcagtttat tttttatttt tgtccgagta gatgccaata
    13141 caaaatctgg atggggaaaa agacggatca atgtcaatgc gtaaggggtc agcctgtttg
    13201 aaaagaactg ttcaagctct ggaaaagtca attgaaggca gttgtgtaaa tccatgcgca
    13261 gacgagtgat tttcttctca atttcttgat aaaaacgggc taaatcgcgc atctctaagt
    13321 aaaaattgct ttgtacttct tttggctgac gttgattttt aagatgagtc tgtgcgagcc
    13381 ggtgggcatc cgatttatct gttttccaac tgcgtaaggt gctttcttcc atctgttttt
    13441 tcgcctctaa cgggtttaac aaacagtaag aaaagtgatg atcttgaaag aacttttcga
    13501 ctggtctgga ataaatcccc gttgcttcaa aaacaatcaa tggacgctca ggcaagtgct
    13561 ggatctcttc gagtaatgca tgaaatcctc tttgagtgtg ttcaatctcg tcttcaaata
    13621 agcaggtgtc gtctttgtat aggacactgt agctgtgtcc cattgaaaca tctaatgcaa
    13681 gaatgtaagg catttgtttt cctccttttt aagttttgaa agaactttac tgcttcattt
    13741 cgattccaat ttcctataca cgggctcatg gccccaacat acgtaaacct gattctaaaa
    13801 tgacagtaaa gaagttcggt ttttgatagg gactcgtagt cctcaaggct ttgttcgaac
    13861 tttctttcac tcttcactat aatctaaaaa tagaaaatag aggagaacat cattcgtcaa
    13921 tgatgttccc ctctaatctt agtatgtttt ttatagtaat tccttcagat actttcttgc
    13981 ctcatcattg aaacgcagtg attcttctaa cttctgataa tattccaacg cttgtttctg
    14041 agcttcagta aaacgataat gttttccttt ccatttgtcg attgtctctt gcacatttcc
    14101 agattggata tctttcacta ctagttgcca tttttctttt gagactgtac ggtctttctt
    14161 atcagcagat tgtatagctt ctcgttcttt acgcaatact tttaaaaaac catttctgac
    14221 tatccgagga atgctgtcga agaacccttc tgtaaaacca tagcgacaat attgcttcca
    14281 aataaataga tagatttttg gttggatgaa tcgttggttt ttcgcaaaat attccatctg
    14341 agcagtgact tctggtgttc gttgacgttt tgtctttctc aacacggaaa taaatgtttc
    14401 aaaataagca ttaggatgta ctggcacttc ttcatatacg atggattgcc aatctttacg
    14461 taaaaatgcg cgatagttga tcaagaattc ttctacatcc tttactaatt cttctgcaaa
    14521 gtcgtcaaat agttcttttt ttatctgtcg cttttttgac tgcccattga tgtgttcact
    14581 tattttcttg atttcatttt ccaatgcttg ataatccgct atgtaaatcg gcatttctac
    14641 ttggcgaaat tctgcgagcc gctgagctaa ttttatatcc ggtaaatatg gctgtttcat
    14701 ccgacgattt tggtacaaga caaaacagta tttttgccat agtccttcag gaaaatcgat
    14761 attcttctgc ttaagccata ataattcgtc taaggccaga tcaacttttg tcattccctg
    14821 atggatcgaa gctgccactg tcggagagac atgcaggtga tcggcaaagt ggttgatgcc
    14881 tagttttagg atctttccgg tttttggcga tttaacgatg tattgcactt ttaattctct
    14941 accacactca caatacaatt tttgctggct tcgttttgtt cgaaaaaacg gattgatctt
    15001 gaagtcagaa aacacccaat ctgttgcagc taaataatgt tcttccataa aacgagagct
    15061 gattagatat cggcggtgct tatcgactag ttcccgctgt tttttcgtta attttgcata
    15121 gatctcccaa cgttgactgc ttgtgtattg tttagaaatt ttgatgggat agcttttgct
    15181 gatttctttt ggaaaccatt cttcaaagac ggattgccat tcttccacgg ctgtttcttt
    15241 cctttctaat tacaaaaagc tggctactaa cataacgtta gcaaccagcc agtgtttctt
    15301 gctaaattag tgagttgttc cggcatttcc ggaaatatct tctttatttt ctactgctgc
    15361 ttcaactgca taagtcaaag aagaagcaat gtcttctaaa gacatagaag gcgtacccac
    15421 tggacgatcg atcacttgat cgggtgagaa tggcacgtgg atgaacccac ctcgaattgc
    15481 cgggaattgt gtttcgatca aatgaagcaa acgatacatg atatcattgc agacaaatgt
    15541 tcctgctgta taagagatgt acgctggtag accatgatct tgtacatttt ttgtcattgc
    15601 tttgattggc aatgaggtaa agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa
    15661 tggctgattt ccttcattat cagggattct tgcttctgct agattgattg cgacacgttc
    15721 gaacgagact gctgaccgcc cacctgcttg tccgacacag atcacgacat ctggttggtg
    15781 ctgttggatg gcttctttga ctacttgact gcttttttcg aatacggtcg gtacttccac
    15841 tttgatgatt tcagcaccgc tgatttcatc tggcattttt tttactgctt cataggctgg
    15901 attcactttg tccccaccaa atggatcaaa acctgttact agtactttca tctgttgttc
    15961 ctccgtccga ttgatttaac atatggttat cttttatcat accattttcc ttgaacagcg
    16021 taaataaagg aaacaatagt ttaaataaca taacagatca aacttaatat attcttataa
    16081 aaaaatagtg ttccactaca atgttagaag aggttgcgac aattattctg tcacaacccc
    16141 tctcttctat tatttaaata gaatcatata agcaatttgg aaaactagca taaataacgc
    16201 aataaacaac tggtttttga tcactccgta tttgtctttc atctccagca tcgctacagg
    16261 cacgatattg aagttagcag ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa
    16321 tgcaaccatt cctattaacg ctggattcgc tccatgacta aatacgaatg gtgcaccgat
    16381 cccaaccgtc atcacggtga tggccgcaaa tgcattcccc atgattaccg taaatatcac
    16441 catgccaaga gcgtaaatga tgatgccgat gttcacattt ccttctggaa cgatcgtacc
    16501 tactgcattg gaaatcactg tcccaactcc agcgcttgta aataccgcac ctagagaagc
    16561 taaaagcatt ggcaacatgc ttaacggccc tactgtgccc agcatatctg ctgcatcatc
    16621 taaaaatgta cttggtcgat tatctcgaga aaagaacatc aagattagca tggctgcaaa
    16681 aacaccgacg cccacgccga ctaaagcacc taaattggta aatagagcaa acaagatcgc
    16741 aaagacaccg atactcaaag ctggaataaa aattttcatt cctagtttgt ctgacatttt
    16801 ttccatatat tcttttgatg gaagccggct ttctcctttg ctgactttct tagcaattgc
    16861 tggtatggtc atcgcaaaga tcaagccacc gttcacgatt gccggaagcc atcttcctcc
    16921 tgcaataact acccctaaga ccgtccagaa aaaggctgac ccataacgat gaggattggt
    16981 tttatctgtt aaatttctta ctccagtgta aatcaatacg agtcccatca cgatataaag
    17041 gatctccagt aatttatcgc ctaataaaat atcaggattt gtaaaaaagt ccatcttatt
    17101 gcccctttcc tggataatat ttcttcgcta atttcttttc tttaagatag aagtagacac
    17161 ttgccacaac taaagccgtg atagccactg gaacttccac taaagcaagc tgtcccaatg
    17221 ttacgtcata tcctagatct ttcagtgttg attggactag caacgcccct gctcctccga
    17281 tgaatagtac ttggccaaag aaccagcaga cattttccat agctgaagcc atacctttta
    17341 gttcttcttc atgcttagga tggacttcta acccttttga ttcgatggtc cccaatgcca
    17401 tcggtaaaat gattggacga acaaatccag caactccacc aaaactcacg ttaaaagccg
    17461 caaagatcat tcgaaagatc ccataaatat ctacaatcat accagcagat actttcttga
    17521 atcgcttgat caaggaagcc gctgattctt tcagaccatt tctttcaagc gttcctgttg
    17581 ccaacataat gatgataaaa atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa
    17641 gttctaacat tccttgaatg ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca
    17701 ttacgatcaa tatcgagtct aatttaaatg caaacccgag aataacaatc aagacgccta
    17761 aaaggctaag ataattttcc atatattttc ctccataaaa tgacaatatt tttttaatta
    17821 taaagcaaaa cacataaaaa atatagttgt tttctcacga aatagacact atttttaaat
    17881 atttttcgtt atttttttat cacaaaaatt atcttactta ttgtaaggta aagctttttt
    17941 taatttattg cccgataata tatagaaata aggtctagtt actgtttttt ttaaatgatt
    18001 aataaataac aacttctttc tatgaaattg caaagcattc atcttctttt ccgctaacga
    18061 tatatccgtc agtgcttggt acactttact aaaaagtatg agattgccga tatctggata
    18121 ggtgtgctat actacagaaa aagatataca atctgccacc gggtaggata aagaggatga
    18181 tcattccatt aggtctttga agggatgatt ttcctctttt tgtgtatttt tttatagtta
    18241 aggaggaatg taagtatggc atggatggaa ttaatcatag caggaatttt ggaagtattc
    18301 tggtcaacga tgatgaaatg gagtgatggt ttttctaaaa tcaattattc tttttacaca
    18361 gtgattggca tgattgctag tttttacttt ttgtcaaagg caatcaaaag tttgccgatg
    18421 agtctctctt atcctatctg gacagggatt ggcgcagttg gttcggtcat aattggtgtg
    18481 gtcttgtttc atgataagct gaatctttcc acttggttct ttgtggggat gctactcatc
    18541 agtatcattg gcatcaagat tactagcggc cactagacac ttggaataag tactgatcag
    18601 aaaaaataag cctttttatt taatattcaa tttttttatg aaaaaataag aaataagtcc
    18661 aatgacctag cgacttctcc actcatcagg tgtatgcttg tactatctat agaaaaggag
    18721 tgaatatttt tgaaaagtct ttcggtccaa gaattatcca agaactttgg tcacaaacaa
    18781 gccctcagcg atgtcagttt tagcttatcc cctggcaaaa tcgttggatt agtaggtcct
    18841 aatggcgctg gaaaaacaac gatcatgaaa gctattttag gactgatcca ttactcagat
    18901 ggaacaatct gtattggtga aaatgagatt tctccctcta gtcataaagg gttggaacaa
    18961 gtaggagcat tgatcgaata cccgggtatc tatccattct taacaggcta tgatcatctg
    19021 aagttattct ctgaaaccaa cgatgtatct gctatcgaca ctatcgtgaa tcagctcaag
    19081 atggaaaagt acattcacaa aaaagcgaaa tcctactcgt tgggaatgaa acaaaaattg
    19141 ggaattgctc ttgccttact gaaccatccg tctatcgtta ttttagatga gccgatgaac
    19201 ggtttggatc cacaagcaac aagagatgtg cgaaaaatca ttaccgactt ggctactcag
    19261 ggcacctctt tcttgatctc cagtcactta ctcagcgagc ttgaaaaaat ggtcgatgag
    19321 ctgatcctga tcaatcaagg aaagatcatc aaacaatgta caatggctga attgaaccaa
    19381 acagcgcaag atttcatcgt tatctctaca acaaatgatc tagctgctcg aacgatcttg
    19441 acagaagctg gatatacttt ggaagatcct gataaaatca agcttttaaa agaaaacgaa
    19501 gaattattag gaaccgtgat ccagctattg tctgcacaaa atatccatgt gacagacgtc
    19561 cagcatctga cgaatgattt ggaatcttct ctattggaaa ttctggattc agtaaagggg
    19621 gaagaagaaa aatgacattg cttgtaaaac aagaattatt taaattgatc aagaaaaagt
    19681 caactgctgt tttatctgtt ctattagttg ttttgctcat aggaacagca cttctagcca
    19741 aaaaatacac gacgatcatt gatccagttg aaatgactgc tcagcttttt agtgccacat
    19801 cttggattgt ctttatcatg attgcagctg caagcacgat catttcgatg gaagcacaat
    19861 atggtacatt gaaaaatctc ctttatcgaa agtattctcg aggagaaata ttagtaagca
    19921 aatggatcac attagttatt tattctgttt atttatacct tttagctatt attgtcactg
    19981 tgttgatgaa actgattctt ttcccttcga tttccttcac ggagaaggtt tctactggtc
    20041 aaacactgat ccaaagcttg ttcacttata ctttgggtag ttatatcggt ttatggctga
    20101 ttttaagttt agtgttgatg attgcttgct ttatcaatag ttccggcgca tccatctcag
    20161 caggaatcgt cttttatttt gcttcgtcaa tcatatctgg tatccttatt gcactcatcc
    20221 aaaaatggga gtggatcaaa tggaatccga tcagtatgtt gaatctgcaa aaccaaattg
    20281 gaaacgaaga gatcatgaag caattgactc atctttctac taatcaaatg ctattcggta
    20341 atttagctta catcgttttg ttcttagcat taggatatct cgtatttaag cgaaaaaatg
    20401 tatgatgact catcaaaaaa gaggctgtga aagagacgtt cagccttatt gccgattggc
    20461 tgtctcttaa acaccgttta ttctgaaaaa ggagctgtga caacatttgt gtcacagctc
    20521 ctttcctagt atgtttttga ataaacgaca ggctcacaac ctctctttat acttctttta
    20581 tacttctata cgtgaagcat cccagtggca aataaaacca ttgcaatcac tccgaccatc
    20641 gtcacaccta taagtaaaat cttatctaag tggatatgtt gtacaggctc gttttgctcc
    20701 ttacgtgctt tgaggtaaaa ataaatgcct ggcaagaaaa gaacaagaac aagcattact
    20761 tcattaaagg ctacgactag catagcccat acttgaaagg cacttgctaa aagccctact
    20821 aaaaattgga cccattcttt ccgctgataa gaaagtttca tttggtacaa tcctaagaaa
    20881 agccacgtga agaaaatagc tgctgtacca agtgtgtaag caaagttata agcactgtca
    20941 gtgaataata atgaaagtaa aaagacattc gtacataacg tcgtcaatac gatagcataa
    21001 gttggtgccc catttttgtt gagttttccc catgctttag gcaaaagtcc gtcttcagcc
    21061 atgtttccca ttgtttcaat cggtaagatc gtccatgaga gccaaacacc aactaaagaa
    21121 atgatcaaac cgccgttgat cacgactgct ccccatttac caaccatcat ttcaaacaca
    21181 tagcccatag caggctgcgc cttgatcgtt tgcagctgat tcatcgtcat tgtcccatat
    21241 ggaagcatgg aaaccaagac gtaaataata atcaagccga atatccctag aatcgttgct
    21301 ttcccaacat ctgattgttt ttttgctcgt gaacctacga cagaggctcc ttcaatacca
    21361 acaaataccc aaagcatcac catgatgcaa ctcaatactt gactggaaaa cgggatcggc
    21421 tgtccattcg cattcaatat aatgtcgcta gaaaagttat caatgaattg ttggatcgaa
    21481 aacgtgctga tagctactac aataaataaa aataatggca ctaatttaca gaatgtgacg
    21541 atagcattga cgaaactagc attttcgact ccccgattca ctagccatgc atatccccat
    21601 aaaaagactg tactcatcac gatcgatgcc agattttgcc caccttcaaa tactgggaag
    21661 aaatatccta atgcgctcat caacaatgtt gcaaaagcta cgttgcctaa ccagcatgtg
    21721 agccagtaac cccaaccgct gataaaacca gcgaaacggc caaaaccttc ttctgcgtaa
    21781 ctaaagatcc cgccttttaa atctggacgt tttttattga tattattgat cgacaaggag
    21841 agcatcaaca ttgctgttcc tacgagtacc caactgaaaa tagctgggcc gccagccgat
    21901 ccggagatgt ccgtcataat accaaatacg ccacctccaa tcgctgaacc aattactaag
    21961 gcagcaagtg gaattaaacc aattcctttt ttctgttgct ctgtatccat gtaagcatcc
    22021 tccaaaataa aaccgctttt atcaaaaagt ataacaaacc ttttttattt ttacttaatt
    22081 aataaaaact ttaatagaaa tataattcta ccggcttttt tctcattata caagcaaagc
    22141 gcctagtccc tttccaaatt acaccaccaa aaaacttaaa aaagatgcta tactaaaagg
    22201 aaagaattgg aggatgatcc atgaacattt tatatatatc gatttcagga aatacgcgtg
    22261 cctttgccaa acatctagca gaatatgctg aaaaaatgca tgcagaagat cctgtcaatc
    22321 ctgaagttac tttaaaagaa atccatgaaa atagtgattt tacgaaagaa acagaaccat
    22381 tttttacttt cgttcctact tatttagacg gcggaaatgg tttagataac ggtgatacag
    22441 aaatcttgac cgaaacgatg cgtgaatatc tggaacatga agacaatcat aaactttgtc
    22501 ttggtgtagt cggtagcgga aataaaaact tcaataatca gtattgtcta acagccaaac
    22561 aatatgcaca aacatttggc tttcctttct tggcagatta tgaattacgg ggaacaccat
    22621 ctgatgtgac acgaatttat cagattttag cagaaaacaa taaataataa aaaagaagaa
    22681 gctgaaaaat agatttcagt ttcttctttc gtttctattg tttctatcat attaagcttt
    22741 gacatccatc acaggtgctt ttgcttttgc tttacctgtt ttttccaaga caaagttttt
    22801 tacacgatcc atattggtta ccaagacgat catggatgtt tctttgcctg ctgctttgat
    22861 cgcttctaag tcagcttctg ctacaagcgt atcaggtgtc acttgctgcc cttctgttac
    22921 tttcacatca aatggcgtac cgcctaagtc aactgtgttg attcccatat gaagcaagat
    22981 ttctaaacca tttggcgttg tgatcccaat agcgtgtttc gttgggaaga cagagctgac
    23041 taccccagta actggagaat agatatgtcc gttttctggt tctacaaagt agccttcacc
    23101 catcatcttt tgtgcaaaaa ctggatcact tgctttatct aaagaaacaa ctgttccgtc
    23161 agcaactgat gagaggatag tcacactttc agctgaagtt tgaggtacta ctttttcagg
    23221 cacttcattt actgtggctg tttcagtcgc taattcttct ttttctaaac ggtctttagg
    23281 aataccgaag aagaatgtag caacgaagcc accagcataa gctgccaata atccaaggat
    23341 gtagcctaac catttaccgt cagagatcaa tgggatcaaa gcggctcctg aaggtccaat
    23401 agcgatagcc ccgacatttc cgatcattcc gatcactgcc ccgccgatac ctccaccgat
    23461 acaagcagta ataaatgggc gtcctagagg taatgttact ccgtaaatca atggttcgcc
    23521 gattcctagg atcccgactg gtaaagctcc tttgatcatt tctgttaatt ctttatcttt
    23581 tttgcagcga atccataatg ccaatgctgc acccacttgt cctgcaccag ccatcgcaag
    23641 aattggaagc aatagcgtac gaccagtttc atcaatcatt tgcacatgga ttggagtcaa
    23701 aatttgatgc agtccgaaca taaccatcgg caagaataat gtgcctagaa cgaagccggc
    23761 aaatgctcca ccaacgccaa gtacccagtt gatcccaccg accatgccat cagaaatgaa
    23821 acctgctaaa ggcatgatga agaaaatttc aatcagaccg atggcaatca atgaaagagt
    23881 tggtgtaacg atgatatcta ccgcatcagg tactacttta tgcagttttt tctcaacgat
    23941 tgacaatagc caaacagcga agatgacacc taggatccca ccttgtccag ctgcaagagc
    24001 ttcaccagta aataagtttt tcagtggtgc ttccggattc atccctgtca gcatcacaac
    24061 ggccccgata actccaccaa gagttggtgt tgcaccaaaa acttgtgcag cattgatacc
    24121 tgtatagata actaggtagc taaacatccc gtttttcaag atgttcatca catcgatata
    24181 ttgttgccat gttgctgcat caagtgttcc ggctgtgact aagtttgcta aaactgcggc
    24241 aatccctgcc acgatcccag taccgacaaa tgcaggaatc attggcacaa agatattaga
    24301 aatgtctttt aataccgatt tgattttaga tggtttttgt ttcgattttt gtgccgcttt
    24361 catttcttgt gcccgttcgt ttacacggtc ttttccagat gctgatgaat ttgctgtagc
    24421 tgtacttaca gaagaaagtt tttctcctaa tttcacgcct gccaaatcaa ccatttgctg
    24481 tgctactttg ttcactttcc caggtccgat gatgacttga agctgttcat cttctactac
    24541 accaagcact cctggaatcg cttttaattc agagacattt acaagatcat cattgatgat
    24601 cttcatacgc acacgagtca tgcagtggac aatgctttct acattgccca tgccgcctgc
    24661 ccctttataa atttgttttg ccagttcttc caaagtcagt tcgttttttg ccatggtctt
    24721 ttccccctca taaggtccct tttacaaatc cgtttgcttc agctaatttt gcttgagctt
    24781 cctctttatc actatttgtc aaaatcatga cgatagccag cttaacattt tgatctgctt
    24841 cttcaaagta cttttcagct gtttcgtaat cagcttctgt cgcttgcatg atgattcgtt
    24901 ttgagcgttc gattagtttt tcatttgttg gtttgacatc gaccatcaag ttattgaata
    24961 ctttacctat accaatcata ctgatagtag aaatcatatt caagataagt ttttgtgctg
    25021 ttcctgactt caatcgagta gagcctgtca aaaattctgg acctgcatcg acttcgattg
    25081 ggaaatccgc gtgtttactg atttcggctt gtttattgca agaaatcgtt cctgtgacag
    25141 caccgatcga ttttgcgtaa tctaatccac cgatcacata tggtgtacga ccgctagctg
    25201 cgataccaat aactatatca ttttttgtca aattcagctt gattagatct tcttgcccta
    25261 gctcataaga atcttcggcc ccttcgacag ctacagtcat tgcttcttct ccaccagcaa
    25321 ttagacctat gaccatttcc ggttctacac cgaatgtcgg tacgcattca gcagcatcta
    25381 acacacctag tcttccactt gttccggctc ccatataaat cagacgtcca ccttttttaa
    25441 aggactcaat cgtgttagca atcactggtt cgatacttgt cagttctttt tcaaccgcag
    25501 cggccacttt ctgatcttct ttgttcatta gttgaacggc ttgagaaata ctcatctcat
    25561 ctaatccaaa agtttcttgg tttctgcgtt ccgttgttag gttctctaaa ttgattttgc
    25621 tcatttttat cttccttctt ttcttataaa ttgatagttc ataccagctt tgatttggtt
    25681 gatcaatggt agctcttctt tgataacttt agctactaca ttcacttttt catccgctgg
    25741 caaatcatat ttggttaatt ggatctcacc catatagcgc aaatactttt cgttatctaa
    25801 agtaatagac cctttcatac gcgtagctgt atttcttgct gggataggcg gaatctctcg
    25861 aaaacgtgca tctacgcttc gtatgacatc tcttgcatcg tcttgccgat tcgtatgctt
    25921 ccctaatata tattcgtaaa attcttcatc tacaggttcc gtatggagca agactgtctg
    25981 ttctttttgg aaagaagcaa attgctcctg aacgttttct gacagacctg catctccgat
    26041 gtaaacaata tccgtgttac ttgctaatag atctagagcg cctgcaagag gatggatccc
    26101 tcgatgttct tccaaagttg gcaatccttg atatagtggg cctctcaact ctgtatctcc
    26161 tggtacaaaa ccttggacaa ggagaccttg aattttgagc cattgatttt ttttctgata
    26221 ccattctttg tccagccctg tttcgggacg tggataataa ttatgccaag cttcaatctg
    26281 tgagaaatca gcttctgctt ctttcaattc atcgatatct ttaggcgtga tcgtgctagc
    26341 attcaaactg attttcaatt gatgtgacca gtcggcaatc tgctgattgg aaatatgata
    26401 atccattctt aaacctgtta caccaattgc tttcaaaggc gttagatctt ctaatgaaaa
    26461 acccgctcgt tccaatgcct ctcctgaaat atcgaccatc aattctagtt tattcttttt
    26521 cgcttcgctt cctagttcga tcaatcgctt ttgatatgcc gaaacatcat cttctggtat
    26581 atgcatggaa gtgaaaatac cgacaaagcc attggatgcc atttggcgga tatactgttt
    26641 cttctcttct gacaaagaat cgttcataaa tattgaaaag ccaaacatat tatccctctt
    26701 tttctgtatc cgtcttcatt tctatgacta gagtatatat ctaaagaaac aaaatttcaa
    26761 tacttttatt aaaataatta aatttcattt ctaaaggttg agtttatatt atcaaaagaa
    26821 ttgtcataac ctaaaaaagg cactgctaag caaaagcagt gccttgaaat attttattta
    26881 aataacacaa aaacataatt actcataaga aaccaattct gtattatcta ccatgaatgt
    26941 aaagaacccc tcattaggca tcggatgaaa ataagcattg atcactctta tcaaggcatt
    27001 atggcaaact aacagataga cattttcttc gtcttcgatg cattctttca ataaaggaac
    27061 gatccgtgca tacacatcta agactgactc cccatttggg aatcgtacag cgaacgctaa
    27121 acgtgctttt tgaaagttct catctttgct tggcagtcca tcataatccc caaaatccat
    27181 ttctaccaat cgttcatcaa cggtcagcgg caagctgaga cgatcagcaa ctgcttgtgc
    27241 tgtatctctt gctcgttgaa gaggggaatg gatgattttc gtgattggaa caggcaaatc
    27301 tactaccttt tctgctagtt tctgtgcctg catctgtcct ttttcagtca gcggcacatc
    27361 tgcccgtccg caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc tcgttacata
    27421 aagcatgtga ttctctcctt cgttggcaaa gttcccctta accatacctt gttgttcttg
    27481 agattacaag ggttagattc tctcaagaga aataatctca gtagatactt tatccaggaa
    27541 ggtttgatca tgttccacta gtaacatggc aggtttcacg ttcaagatca gttgttctaa
    27601 ttgttcttga ttgaagacat ccaaataatt caatggttca tcccatgtat atagttcagc
    27661 tggctgtgat aaagatttag ccaattccac ttttttccgt tggcccatac tcatctgctc
    27721 gatcttgtta tgaaaaacat ctctttccat cccaagcttt cggaggttgt tcaaaaatgc
    27781 ttggtagtct acttggtttt tctctgcaaa ttccgccaac gttcctcgat tgtcttcata
    27841 attttggctt gcataactaa tgctcagatg ttttggctgg tatttttctc ctataacctt
    27901 gccgttgaat gcccccagaa gataatggat aatggatgac tttcctgcac cgttaggacc
    27961 tgaaatcgcc acccgctgat gaggctcgat tgtaaaatga attggctcga ataacagatt
    28021 ttcataccct aattgaagat cttctacgct taaaagtcgc ttatggtgag acgcttggct
    28081 attcatcgtc aaagaatcga tatattcgat atcttttaat agtttttctt tctcgctgat
    28141 ctgggtctcc atccgattca cgattgcttt ggatcgtttc atcgtccgtg cagcatcagc
    28201 acccactgct cctttattca ctcgtctaga ttcagtatcg atgaatccga cttgtttctt
    28261 tgttttatct ccttctcggg aacgagacca ttcggctttt tcagctgctg ttttctttag
    28321 cctactgact tcttttttca atttttcatt ttgagccatt tcaaattcat cacgaagttt
    28381 tttctgttct tcatagatag agaaattccc ttgataaagt tccagttgac ttttttcgat
    28441 tgctaaaaca tggtccacta cttcatcgat aaatccccgg tcatggctga ccacgatgaa
    28501 gccttgtttt ttctttttca aataagccgc tacttgtttt ctaccagaga tatccaaatg
    28561 attcgttggt tcatcgatta acgggaaatg agtgtcatcc acaaataaaa gtgccagtag
    28621 gacttttgtc ttctctcccc ccgatagtgt gctgaattct ctccataaga tttcaggatc
    28681 tgtttgcatc aattggagct ctctttcgat ttcccatatc tcaaaatccg taatatcatt
    28741 taacacgtaa taggttaaac gttctttatc ttttgtctgt tgcgggaaat aggcaaattc
    28801 ttgctgatgg attacttgcc cttgataagg tagtttgttt tgtagaatat tcagtaaagt
    28861 tgtctttcct cgaccgtttc gtccgataag tcctagtttc cattgtgtgt caaaatttag
    28921 atttgcttgt tcaaataata atgtgccttg gctgtcgtag ccgaatgtca gattttttat
    28981 ttcgatttta gacattttcc atcacctcgt aaaaaaagac cagtcctatt ttcagaactg
    29041 gctaaagagg atgcaagggt tggatacaca caaaaagacc catacatcga aaaaaccaag
    29101 ccaatcccga aaatctgcac actgcttcct tgtctccaca aggtacagtc tgaatgcagc
    29161 tttcatcaat cgacttaatt tttcaatgca cggatccctc actttgtcct atatttgaaa
    29221 ttacgatagc ataaagaaaa tgattttgtc aactacttcg ggaggatggt gaaaaatctg
    29281 ctaagtgtct gtcctaagat ttttgttttt tctaatgttt gcctgtgttc cattgcttct
    29341 tctaaaccta ttggacccaa atgaatactt aagacaattg ggagaaaatc agaaagtcct
    29401 tctagctctt ggtctctact tccgcaaata gcgattattg gttttcgata tttttttgca
    29461 agcactgcta caccgtatgg aacttttccg tggatcgttt gctgatcgat ccgcccttct
    29521 cctgtaatga tgacatctgc ttgttcgatc tttttttcca atccagtgat ttggctgact
    29581 aaatcgaaac cagaaaccat cttcgctccc aacaaaaaaa gggctccacc aataccgcct
    29641 gctgctccac ttcctggaat atcatctaaa tccacctccc agaaatcttt tatttgctga
    29701 gcaatctttc ctgcttgttt atctaagaaa gctagcgtcg tatctattcc tcctttttgt
    29761 cttccaaatt gccaagcagc tccttgttga cctgtatatg gattggtaac atctgcagcg
    29821 ataatcaatt ttactttaga aacaagaacg tttcttcgat caaaatgaag ttttttagta
    29881 gataataaag gatttccttc tgtgtaccct tctaaagaag cacccaagga ttggagtaat
    29941 cctagaccac catcgctaca cccgcttcct cctaaagtga caataatttg ttctacttct
    30001 tgaagcagtg catctttaat aagttctcct aaggcataac tggaagcaag tcggatagtt
    30061 tgatccgttg gtttatcaag tagatctaac cccaatgctt gtgcagattc gatgactgcg
    30121 gtgttttttc cttgccaagt tgtcaataaa tagctgattt ttcgctgacg gaataagaga
    30181 tccatcccat caatggtacg ccatgttccc ctgagcgcgt aatagattgc ttctgcagta
    30241 ttttctccgc catctgcaat cggcacattg atttttcttc ctttacgatc ttcccagcca
    30301 tttagaactg cctgatttag ttcaaatgaa gaagcacttc ctttaaatga atcaattgcc
    30361 gccaaaatat ccatctgtat ccctcacttt cttcttaagt aagacacttt ttttcatttt
    30421 tttcaaacaa aacaatgcta ttttcttcca tttttctata tactaaaagg gaactcattg
    30481 aataaaggag agaaaaaaca tgaaaatcgc aatagctggg gccggagcaa tgggcagccg
    30541 aatcggattg atgctacatc aaagcggaaa tgaggtcctc ttgatcgatc gttggcctgc
    30601 tcacattgaa gccattcgta caaatgggtt gattgctgac tttaacggta aagaagtggt
    30661 cgcaaaactg ccaatctatt caccagaaga aatcatcgaa agcaacgaac acgtggactt
    30721 gatcgtagca ttaacaaaag ccaatcaatt agatgatatg ttttgttcga tccaatccat
    30781 tattactgac aatacttatg ttttgtgttt actaaatgga ttaggtcatg aagatgtact
    30841 ggaaaaatat gtgccaaaga aaaatatctt atttgggatc acgatgtgga cagctggtct
    30901 agctggtccc ggaaaagtca cattgttagg tgatggagaa atcgaactag agaacttgga
    30961 accagaaggc gaagcattta caaaaaaagt tgtagaagtc tttcaagaag caaatttaaa
    31021 tccgatctat agccataacg ttcgttattc catttggcga aaagcttgtg taaatggaac
    31081 attgaatggt ttatgcacga ttttggattg taatattgct gaactaggcg cgcaaaaagc
    31141 ggcagagagc atggttcgaa caatcgtatc tgaatttgct tctatcgcag caaaagaagg
    31201 aatcatacta gatcaagaag aagtgtacca acatattgct tctacttatg atcctgacaa
    31261 catcgggttg cattacccat ctatgtacca agacctgatc aaaaaccatc gtttgactga
    31321 aatcgactac atcaatggtg ccatttggcg aaaaggacaa aaatatgata ttgctacacc
    31381 atattgtgcc tttttgaccc aattagtcca tgcaaaagaa ggaatccttg gcgcagaata
    31441 aaaggctctc tgatgagaat cagctgaatc agcagctggt tctctttttt taagcccaat
    31501 aaaagatcaa aaagaaaaca caactctcat gatgccataa gaattgtgct tgtttgtttc
    31561 agtacataat ctcttcaaaa tgtaggtttt ttatttgttt tcaacagagg agaggtcata
    31621 ttcgtattgc tcataaatag tggaatcgtt agtgataaat cgttttgccc aattttctgc
    31681 atcataaaat tccaatagct ctgataggat tggcttaaat ggtgtatgca gaaattccgg
    31741 taattcatct aaatcacgtt ctcgaagcaa aaaaatattt tcaggacgat aaaaatgata
    31801 atgagcaatc gcttgattat ccgtcactag tttcttttga aaatacatgg attcaaatgt
    31861 ccgcaaagtc actccttgct gaccctcccg caacagttcc agaatcccgc gcgattcccc
    31921 tgtttttttc aggtactctt cataagtaag atatccaggt tgttcattcc ctcgatgtgg
    31981 aagaatgaaa tagttggtcg taacatctct ttttataaat tcctgcttat agctttctgc
    32041 tcgttctttt ctcccattat tcgttgcacc aaaaaataga tccgtctttg gttcgctaga
    32101 agaaaaattc atatgtttcg aataaaatga actattatgt ttcagcccaa gttcttttgc
    32161 ttccaaaaaa tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag
    32221 catcgcctcg tgttctgctt tgaagtgatt ccaaaaaaat aacacaattt ttgcattggt
    32281 aaatgggcgc acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag
    32341 ctgataattt gccaactgtt gtctagctga tttaatgaat tccgactgta gtaaatcttt
    32401 tccggaccaa acatctaaag agcggatgga aggcaaaaaa taaagtgatt tattttttgc
    32461 aactagcagt acgtcatttt tgttcatctt ttccgcctca ctttctctgt cactttttcc
    32521 tatgatagcc tgtttaaata gtaaaaagcc agtaaaacgt ttcctatatt tgtatgaaaa
    32581 tttaacattt gtatgaaaat atgatgaaat aaatcattct tctattaaaa ttcgcctaaa
    32641 agttttctga atagttgtaa ttgtctgaaa atttgttatc attagtcatt gttaaatatt
    32701 gctatttatt cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga
    32761 agataaaaaa ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg
    32821 agcaatcggt gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc
    32881 tgtattgatt gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg
    32941 aaaaatcgtt ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta
    33001 tctaggtgct cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa
    33061 tatcatcgct gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc
    33121 agcctgggta tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt
    33181 acggttattc gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt
    33241 actgatcatt tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc
    33301 tacccttcag caattgactg accatggtgg gtttactcct catggtatga agggaatcat
    33361 cagttctttg cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt
    33421 aagcgaagca gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag
    33481 aatcatttcc ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac
    33541 tttagcaggc acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt
    33601 tgcagcggat atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg
    33661 tgtgtatgca tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag
    33721 aaaactagct gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag
    33781 tgttctttat cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct
    33841 ggcaggttcc ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt
    33901 tgtactggca ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc
    33961 tgtcttagga ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt
    34021 gactggcctg ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt
    34081 attgacgaat gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc
    34141 tattgaagaa aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt
    34201 ctcatgacct cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct
    34261 ccatgctcat gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa
    34321 agtattggaa gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt
    34381 acttgaatgt ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt
    34441 tgatgatggg tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa
    34501 acaaaaagat aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt
    34561 ttatggtacg aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc
    34621 atcattctat cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa
    34681 aattagcgga agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga
    34741 tcaaagaaat ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta
    34801 tcagcttcaa gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa
    34861 aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg
    34921 tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta
    34981 aaatcagcgt tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt
    35041 gccatttttt gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt
    35101 gaaaaagtcg cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt
    35161 tgtataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg
    35221 gattgtgaca aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg
    35281 tcttgcttac tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg
    35341 cactgaccat cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga
    35401 gtgtaggaat accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt
    35461 agttgattac tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg
    35521 ctttttcata agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac
    35581 caataaaaat atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt
    35641 tcgtcatctt tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca
    35701 atccacctaa tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc
    35761 ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt
    35821 cgatgacccc agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta
    35881 ctgctacagc gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca
    35941 gtaacattaa aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa
    36001 catcagtcac ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca
    36061 tgaataaaaa gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt
    36121 gtttgaacaa atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag
    36181 acatcttatc tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta
    36241 agcctagtgt tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg
    36301 cactccctac agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact
    36361 ggacactgat caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc
    36421 atacagcaat caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga
    36481 tgccattgat gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca
    36541 acatgaccat tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa
    36601 cgccaatcgc aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg
    36661 gtgttccttt ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa
    36721 gttaagatat cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa
    36781 aagacctgtt ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat
    36841 ctgtttttca ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt
    36901 aggcttgatc ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc
    36961 gtgaaaagtt gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa
    37021 aatatttttt catttttctg aaaattattt ttataacttt cacacacaag attatttttt
    37081 actagaaagt agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga
    37141 ttctttctaa tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg
    37201 ctgcaatcgc aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg
    37261 tgtgtccggc aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt
    37321 ggctgacatt tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt
    37381 catctggaac acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa
    37441 cgatgacatc atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta
    37501 tcgcagcacc tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat
    37561 ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc
    37621 ctccgtctgt aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt
    37681 tgattccatt aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg
    37741 ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt
    37801 ctgtcgtttt cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa
    37861 aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg
    37921 gggtgccata tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg
    37981 cggctaatac ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta
    38041 aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg
    38101 catcaaaggc tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta
    38161 attgtttaat caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt
    38221 ggatatctag agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt
    38281 ggtttgttga gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt
    38341 ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt
    38401 tgactgtgtc ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat
    38461 cttcttttct tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta
    38521 agaagatggc atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca
    38581 ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt
    38641 ctagattttt tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat
    38701 ccactttgat caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat
    38761 cattagggat tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag
    38821 aataaattcc cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt
    38881 ctttattgta attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat
    38941 ctgctaaagc cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt
    39001 tctccttttt caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc
    39061 ctagttttac gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg
    39121 ccaaccaaat aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt
    39181 ctcctccact ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa
    39241 aaaactaaga ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta
    39301 taccctctcg atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta
    39361 gtcagtccga tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct
    39421 tctaacaaat acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct
    39481 tcgatcaccc caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga
    39541 acaactccaa aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag
    39601 aaacgagtag tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata
    39661 ttgatcagct tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt
    39721 gtcactacta acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa
    39781 aaagacatat atagtgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca
    39841 ttgggcaaat atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat
    39901 acgactatct aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata
    39961 atccaatgct tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa
    40021 cggcgtttct tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt
    40081 gctgtacaga tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac
    40141 cagccattta ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc
    40201 ttggtcaaga tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct
    40261 gaagatctgg atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt
    40321 gttgcagatt tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa
    40381 ctggcgattc aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc
    40441 actggttgct tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc
    40501 tactcgttgt ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa
    40561 accgacaagt tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga
    40621 atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg
    40681 ctgaaagatc ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact
    40741 cagattcgtc tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac
    40801 acgaactaac gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt
    40861 ttcaatcgtt aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca
    40921 tacagaaaca tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc
    40981 aagaaaatga aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc
    41041 aagctgtgtt cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc
    41101 gcagccgtca tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat
    41161 agtcgttcct tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac
    41221 ttaccatttc aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt
    41281 cagtaaaagg acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc
    41341 caacatctca aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc
    41401 tacttatcga caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca
    41461 ccagccagag cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat
    41521 tttgttgttg ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt
    41581 attttttcta tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat
    41641 cttttccttc cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa
    41701 aactggaatc aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta
    41761 ctaaagaatc ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg
    41821 tcagataagg aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt
    41881 ccaagtaatc agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg
    41941 tcgtagcaat gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat
    42001 atggttttaa gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg
    42061 ctcggacttc ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt
    42121 gtttccagtt ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt
    42181 gctgaaatcc ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat
    42241 ctaaatcaaa tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag
    42301 tttagcacga ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag
    42361 ttcttggaat cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat
    42421 gactgtagca aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc
    42481 accaaaactt gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa
    42541 catggcaaat gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag
    42601 cactaaggtt tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa
    42661 aatcactcgc tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat
    42721 aacaaaatac atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta
    42781 aaccatgaat tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt
    42841 atccatccga tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa
    42901 aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa
    42961 catattgcca aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt
    43021 ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac
    43081 gtctgcagat tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac
    43141 tttcattgca ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt
    43201 acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg
    43261 gtcttcagct acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac
    43321 caattcattg atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc
    43381 cccagtcaga acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt
    43441 tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc
    43501 tttgactgaa aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg
    43561 taagccatct tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt
    43621 taatggtgta acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat
    43681 cgcacctttt gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg
    43741 acgacgttca aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc
    43801 taaagtttct gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg
    43861 atagtagcta tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc
    43921 catatgatat tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa
    43981 cacatcgatt gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt
    44041 ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg
    44101 tgttagacca actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt
    44161 aagcccattg atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat
    44221 ggatgtttta tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc
    44281 tgctacatga ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc
    44341 actgatgaca ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc
    44401 gtcttcacat actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg
    44461 ggaaatgaat aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc
    44521 tgaaagccga acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc
    44581 attacgtttt acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt
    44641 ggaacgtacg gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat
    44701 ggctacaccg aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga
    44761 aataattcct aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc
    44821 tacaatgaat ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga
    44881 acgttcgatt tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc
    44941 ttgtttcgtt tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat
    45001 ctcaatctct cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat
    45061 tcatcggatc gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg
    45121 cctttcctct agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta
    45181 ctaagtaatt ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga
    45241 ccacctcctt tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa
    45301 atactcagcg aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga
    45361 gttttgacac tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc
    45421 ttatctcgac aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct
    45481 atgtttgtat aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca
    45541 actaaaaaag aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac
    45601 acgttatatt cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata
    45661 gaaatatagt aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga
    45721 cggcgtgaga cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc
    45781 cattgctgaa atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca
    45841 cttgactgag cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt
    45901 ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac
    45961 aaaagctgcc gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga
    46021 aaacatcatt cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt
    46081 cccgccattc gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac
    46141 tgctggtcta gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt
    46201 gattctttcc taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt
    46261 cttcccctaa agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga
    46321 tcactgtact tctttcttta tgtgctaagc tccgtttcat atcttcattc acactacgtg
    46381 cctttttcaa ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga
    46441 gatactgctc atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat
    46501 aattgttctc aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt
    46561 caaattgacc atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac
    46621 gtaaaaattc aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa
    46681 ccaaataata cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat
    46741 aaggatcaaa gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta
    46801 ttggttcatc cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct
    46861 ctaaaaatga tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga
    46921 ctagagaagt cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga
    46981 ctcgaagttt cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt
    47041 tttccacatt caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa
    47101 tgtttttttc ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg
    47161 ttggtttgat tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat
    47221 ataagttggt tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt
    47281 tttgcttttc gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat
    47341 caatcgtctc aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat
    47401 ctgaaagact taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc
    47461 ctgctgcttt cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga
    47521 cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag
    47581 aagataataa gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta
    47641 gtaatgtcgg caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat
    47701 aaccaggcat atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc
    47761 atgtatctgg atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc
    47821 caaaatgatc cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat
    47881 acgtaaaaaa ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta
    47941 ctgtgctatt gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag
    48001 gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat
    48061 attgggcaga ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg
    48121 attcatcaaa ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat
    48181 ctctattcca aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt
    48241 gtccaaggat cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg
    48301 agccttgagg taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc
    48361 cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac
    48421 tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga
    48481 aactttctaa atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat
    48541 ttggctctgc ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg
    48601 catgcgcgcg aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat
    48661 cgattcctag atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac
    48721 ggttggcttc gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag
    48781 cgatagccat cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc
    48841 aaaccagtag cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca
    48901 tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt
    48961 tgactgtaat gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg
    49021 tatttaaaat atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca
    49081 aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact
    49141 ggatcgtacc ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc
    49201 agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt
    49261 gtgtcacgta tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga
    49321 aaatttccta aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt
    49381 ccacttttca cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa
    49441 gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca
    49501 aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct
    49561 ttttcacaac ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa
    49621 tatttataaa acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg
    49681 gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat
    49741 gtcaggacgc taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt
    49801 aataagccca accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct
    49861 cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccttcg
    49921 gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac
    49981 tcaggtcaaa acgctgattt cacaacctct gtttttcatt gtccatagga ttgatattgt
    50041 ccttgtgatg actcattcgt aataattgag gcttgatttt cttcacttcc cgttatcgtt
    50101 tgtccactgc cagaatctga aggacttgtt cctgtcgact cgtttttttg agaagagaca
    50161 gttgttttct tgacttgttc atttgtcttt ttccattccg ctaaacgatc ggcgaccact
    50221 tttttgtcta ctttttcaga ataagcggtg atcacttttc catttttcaa acgggctact
    50281 ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa ttgatattgt
    50341 ctgagagaaa tcgcactaag tacgtcatcg atattaataa attgattttc ttcttttcct
    50401 tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt atactccgcc
    50461 caccaataag ttgagaattc ttttttgaaa ttatctactg attctttgct tgctacgatt
    50521 ttttctgttt ctttctgagc aagatctgct gtcatttttc ctgaaaatgc tgcggataaa
    50581 ataagcagta agaaaaaacc gaataaccaa aagagaagaa acggtctttt tttcctcatg
    50641 ctttcctcca ctcctattta attttgacta ttctattata tacaaaaaat gaaatagaaa
    50701 gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa atggtaagaa
    50761 tggagaattt cacgcatttg ctctcaacta ttctttctta agcctcaaat ccgtttttat
    50821 ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt tctttttcca tcttgtgcta
    50881 agtctactga aataaagcca tatcgattct tatatgcatt ggtccacgac cagttatcca
    50941 tacatgtcca catatgatag cctaaacaat tggcaccttc ttgaatggct tgatggacat
    51001 atttcagatg agattggaca aattcgatcc gataatcatc gtggatcatg ccatcttctt
    51061 tgataaatcg tgcttcgttt tctacaccca taccgttttc agaaataaag cagtcgatat
    51121 ttccataatg atctcgtaca ttgatcaaaa tatcatagat tccttgttca tagatctccc
    51181 atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaaa taatcctcag
    51241 gcattggacc gtttttagta tcgattggtg tttcttttgc ttttacccgg cgtggctggt
    51301 aataattgat acctaaaaga tctactgtat tttctttgat aaccgtcaaa tcctctgcat
    51361 ggatcacagg catgagatca agttctttta ctatctcaat caattcactt ggaaactcgc
    51421 cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt tgcgctgctt
    51481 taacatcttc agggtttgat tcgtctcttg gatagcttgg tgtcagattc aagatgatac
    51541 caataacacc accttgctcc atttcatggt aagatttgat ggctaacgct gaagccaacg
    51601 cttcatgata gccaacttgg atcgcttttt tcatatctga ctcttctgga taatgccatc
    51661 gatataaata acctgcttcg actggaacga ttggttcatt atgagtaaac cattttttta
    51721 ctttcgaacc aaacaattca aaacaacttt tggcatattt ttcataggct tcaaccgttt
    51781 ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa tgatacagat
    51841 tcacaaaggg ttcgataccg ttttcaatca actcatcaaa ataacgatga tagaaacgaa
    51901 cggcttcttc gttgattgtt ccagattcat caggtatcaa acggctccat tggatagatg
    51961 ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga tacttcgtat
    52021 aaacttgcga ggttttttca ggtcctactt gatcaaagaa cttttgcggt tcttttttgt
    52081 accaataatc aaaaatgttc tctccttttc catcgcctga aatccttcct tctgtttgtg
    52141 ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt ttcatcaaag
    52201 cacctctttt tcttttttag ttcgttttag ttgattccat gtatgatatt ttccatcaca
    52261 atcacttttt cattttcttc ctcttctttt ttgattcgtt ttaacagctg ttctgctgct
    52321 cgttccccga ttttatctgt tggtggcgtg atcgtgattt tcggaaggat actcagatcg
    52381 tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct
    52441 tgattgaaac attcgatggc accaatcgac atattgtaat tgctgacaaa aatagcagtt
    52501 gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct
    52561 gtaaagacct ctaattggat cgatggatca ttttggaccg cttctttggc tcctgctaaa
    52621 cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa atccgatttt tttatgtcct
    52681 tgttcaccat gatgagcgat cacttttgct acactttctc gatcattcac taatattgta
    52741 tcaatacctg ctggtccttc tggacaattc aatgcgataa ctggaatact catttttgtg
    52801 agcatttcaa atccagacca ctcttcttcg gataagaaga caatcagccc atcgatcaca
    52861 tgcttcatca ggtattcaat tttttgttca attttttctg gctgatcggc aaacccgctt
    52921 aataataagc tatacccatt ttcttcagct actctttcga tccctgagac aacttccgac
    52981 ccaaaacggc tggaaatccc gtttatcaaa agtccaatag agaagcttcg attattcttc
    53041 aaccctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgctattttt
    53101 ttcatgttct tttccttcag ttgctgacca ttcaagtaac gggaaactgt tcccactgaa
    53161 acaccagcca tcttagccac atcacggatc gatgccatac attctcactc ctgccagtta
    53221 catttgattt ctctttcttg ttgttcgatt atgatgaccg cattctttgc atagtgttgc
    53281 ttgtcatcaa tatccatata actaaagtct tcagtagagc aacttcctct cactattaat
    53341 tatacttaaa caagtatttg aaccggttca tttttaatat agcatttttt taaaagattg
    53401 caacactttt ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt aaaattttat
    53461 tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc cgtttgaagt
    53521 tttacattcc aatggtataa tttctttatc ctatcaaatg ggggaagtat tttgaagaaa
    53581 ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg tgatttgttt
    53641 ggcggctctg accaatcctc ggacgaaaca accgattctt ctgtcttaaa agcggaaaaa
    53701 gcagatacca ccaacagtgt atatacgcta aaagtacgta aagatatgga tttatccgcg
    53761 ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca gacacaagga
    53821 actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa gatcactacc
    53881 aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaaacaac agtcatattt
    53941 acaaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag tctatagtgc
    54001 cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa gaagcaagat
    54061 gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat gtaataacaa
    54121 caaaaatatc catacagaat ttaatgacca aggaagcttg agcatatgcg acaactgtca
    54181 caaactagtc aaagattcat ttaaaacatt tgaatcttat cctgttcaaa attatgtaaa
    54241 aagtagaaga agataaacaa atccaagcaa aactcactat ggctgatttt tatgaacttc
    54301 aaactcagct tcttcctttg atcaagcttc atcagtaaaa caaacagtgg atcatttcag
    54361 tttacaaatc aaagagtaaa gatttattta tttcaaatat ttgaatataa aataaccaaa
    54421 cgatagaaag gcttattcta catcacgtag taat
//
LOCUS       DACDHL010000011        51089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDHL010000011 DACDHL010000000
VERSION     DACDHL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.011"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(22..1722)
                     /locus_tag="IV228_000683"
     CDS             complement(22..1722)
                     /locus_tag="IV228_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716622.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2229..3389
                     /locus_tag="IV228_000684"
     CDS             2229..3389
                     /locus_tag="IV228_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8716623.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3405..3848
                     /locus_tag="IV228_000685"
     CDS             3405..3848
                     /locus_tag="IV228_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716624.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4331..4861)
                     /locus_tag="IV228_000686"
     CDS             complement(4331..4861)
                     /locus_tag="IV228_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8716625.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4966..5517
                     /locus_tag="IV228_000687"
     CDS             4966..5517
                     /locus_tag="IV228_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8716626.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5670..6143)
                     /locus_tag="IV228_000688"
     CDS             complement(5670..6143)
                     /locus_tag="IV228_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8716627.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6281..7753
                     /locus_tag="IV228_000689"
     CDS             6281..7753
                     /locus_tag="IV228_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8716628.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7750..8460
                     /locus_tag="IV228_000690"
     CDS             7750..8460
                     /locus_tag="IV228_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8716629.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8812..9096
                     /locus_tag="IV228_000691"
     CDS             8812..9096
                     /locus_tag="IV228_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8716630.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9413..9796)
                     /locus_tag="IV228_000692"
     CDS             complement(9413..9796)
                     /locus_tag="IV228_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8716631.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9798..10382)
                     /locus_tag="IV228_000693"
     CDS             complement(9798..10382)
                     /locus_tag="IV228_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8716632.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10429..11904)
                     /locus_tag="IV228_000694"
     CDS             complement(10429..11904)
                     /locus_tag="IV228_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8716633.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12085..12396
                     /locus_tag="IV228_000695"
     CDS             12085..12396
                     /locus_tag="IV228_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716634.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12511..14847)
                     /locus_tag="IV228_000696"
     CDS             complement(12511..14847)
                     /locus_tag="IV228_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8716635.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            14989..15657
                     /locus_tag="IV228_000697"
     CDS             14989..15657
                     /locus_tag="IV228_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8716636.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15660..16703
                     /locus_tag="IV228_000698"
     CDS             15660..16703
                     /locus_tag="IV228_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8716637.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16940..17821)
                     /locus_tag="IV228_000699"
     CDS             complement(16940..17821)
                     /locus_tag="IV228_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8716638.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17980..18708
                     /locus_tag="IV228_000700"
     CDS             17980..18708
                     /locus_tag="IV228_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8716639.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18864..19181
                     /locus_tag="IV228_000701"
     CDS             18864..19181
                     /locus_tag="IV228_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8716640.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19174..19782
                     /locus_tag="IV228_000702"
     CDS             19174..19782
                     /locus_tag="IV228_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8716641.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19798..20628
                     /locus_tag="IV228_000703"
     CDS             19798..20628
                     /locus_tag="IV228_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8716642.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            20766..21278
                     /locus_tag="IV228_000704"
     CDS             20766..21278
                     /locus_tag="IV228_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8716643.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            21304..22176
                     /locus_tag="IV228_000705"
     CDS             21304..22176
                     /locus_tag="IV228_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8716644.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22352..22714
                     /locus_tag="IV228_000706"
     CDS             22352..22714
                     /locus_tag="IV228_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8716645.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22723..23475
                     /locus_tag="IV228_000707"
     CDS             22723..23475
                     /locus_tag="IV228_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716646.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23489..25291
                     /locus_tag="IV228_000708"
     CDS             23489..25291
                     /locus_tag="IV228_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716647.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25568..26608
                     /locus_tag="IV228_000709"
     CDS             25568..26608
                     /locus_tag="IV228_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8716648.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26660..28804)
                     /locus_tag="IV228_000710"
     CDS             complement(26660..28804)
                     /locus_tag="IV228_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8716649.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(29161..30534)
                     /locus_tag="IV228_000711"
     CDS             complement(29161..30534)
                     /locus_tag="IV228_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8716650.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(30534..30929)
                     /gene="rbsD"
                     /locus_tag="IV228_000712"
     CDS             complement(30534..30929)
                     /gene="rbsD"
                     /locus_tag="IV228_000712"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8716651.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(30957..31874)
                     /gene="rbsK"
                     /locus_tag="IV228_000713"
     CDS             complement(30957..31874)
                     /gene="rbsK"
                     /locus_tag="IV228_000713"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8716652.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(31982..32995)
                     /locus_tag="IV228_000714"
     CDS             complement(31982..32995)
                     /locus_tag="IV228_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8716653.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(33076..33675)
                     /locus_tag="IV228_000715"
     CDS             complement(33076..33675)
                     /locus_tag="IV228_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8716654.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            33861..34562
                     /locus_tag="IV228_000716"
     CDS             33861..34562
                     /locus_tag="IV228_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716655.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            34572..37283
                     /locus_tag="IV228_000717"
     CDS             34572..37283
                     /locus_tag="IV228_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8716656.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            37734..39362
                     /gene="treC"
                     /locus_tag="IV228_000718"
     CDS             37734..39362
                     /gene="treC"
                     /locus_tag="IV228_000718"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8716657.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(39422..40603)
                     /locus_tag="IV228_000719"
     CDS             complement(39422..40603)
                     /locus_tag="IV228_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8716658.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            40855..42378
                     /locus_tag="IV228_000720"
     CDS             40855..42378
                     /locus_tag="IV228_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8716659.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(42447..43175)
                     /locus_tag="IV228_000721"
     CDS             complement(42447..43175)
                     /locus_tag="IV228_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8716660.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(43188..44468)
                     /locus_tag="IV228_000722"
     CDS             complement(43188..44468)
                     /locus_tag="IV228_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8716661.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(44609..45211)
                     /locus_tag="IV228_000723"
     CDS             complement(44609..45211)
                     /locus_tag="IV228_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8716662.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            45456..45662
                     /locus_tag="IV228_000724"
     CDS             45456..45662
                     /locus_tag="IV228_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716663.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            45974..46693
                     /locus_tag="IV228_000725"
     CDS             45974..46693
                     /locus_tag="IV228_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8716664.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(46774..47457)
                     /locus_tag="IV228_000726"
     CDS             complement(46774..47457)
                     /locus_tag="IV228_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8716665.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            47676..49862
                     /locus_tag="IV228_000727"
     CDS             47676..49862
                     /locus_tag="IV228_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8716666.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            50208..50618
                     /locus_tag="IV228_000728"
     CDS             50208..50618
                     /locus_tag="IV228_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8716667.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            50632..50988
                     /locus_tag="IV228_000729"
     CDS             50632..50988
                     /locus_tag="IV228_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8716668.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        16640 a         9010 c        10177 g        15262 t
ORIGIN      
        1 ggatgtattt atcccttttg cctaagcacg taaaagcttg tatctatgat aaataaataa
       61 acatagcaag gtaaaccagc caatcaaagt aagagaaaga atgaaataga aatagattgg
      121 cgtttgataa tgaagcgtga gctcgttgat tcctttttgc tgcttcacga ttggggtccc
      181 gatatctgtt acttcataat cagtcaatgt ttttccattt tgctgtagca cagtccgatc
      241 gtagacgata acagggatat gaacagtttt gtccccagtg tttttccact tgattgtcaa
      301 aagaccgcct ggatgtgttt ttataaaagt tgtttcgttt tctagtattt cttcgttata
      361 ccggtcgtat ttattgtctt ttgtctcctt atagattggc agatagtcag gtgtggtttt
      421 ttggaaaacg aacaagctgg tagacatatc tttgctgaag aatgttttcc ttgcttcttc
      481 cggcgtatcg aatagataag tatgtcgact gacaaatgtt tcattttccc acttttccag
      541 atgctggtat aagttctgga tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa
      601 aagacggaac cacttttgtt cagaccactg gttcagtgaa agggcaaggt aaagtagaag
      661 caatagcgta aatggcacga aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac
      721 atttttttcg atcaatgtcg tccatggaat caagctagta gaaagaaaaa agaaaacaag
      781 acttgtgtac aacacgattt tttggaataa ggtgttcttt tgttgaaaga aaataaaaag
      841 caaaaaacct gataatatca atataggaaa ggccttaggg taatggagcc aataactgcc
      901 atccagatct atcgcctttt gactcatttc tctgttaaca aagggaggaa gaatcttatt
      961 cccactgtaa acaatagcta atcctgccca aatatttgct gtcagaacaa aataaagcaa
     1021 gaccgactta atcagttgaa gcagtgcttt tcctttttca gtctggttaa aaaagtaata
     1081 aagataaaaa gggatataca tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt
     1141 cataagtccc actgagacag cgactttgat aacagggaac tttttatgaa ggacgaaatc
     1201 agcaataggg atcatacata aaggataaaa acaagtcccc cagctagtga atccttggtt
     1261 gatagaccag tattgaatag aatacgtact catgaataaa acggctactg gtacacttac
     1321 ccaatttcga acattgacat atctcagtaa aatcagcata gaaaatcctg aaataagata
     1381 aagaatgaaa tttgaaagaa tctgataatg aaaccagcta cctgcaagca acacgatcaa
     1441 tccttgtaaa taagcgaaaa atgggccgta aacggcattc acgatcctgc cactttgctg
     1501 gaatccataa agggaaggaa aataatggaa atccccatat ttaatttgtt gcgcggcatc
     1561 atagaaccga ttgtaatgga atggggcatc agcacccaaa attagtttat gtgtgaataa
     1621 atgaggcatg agaaatgcca aagcaatgaa aaaaattgct aaaaatgggc catatttttt
     1681 caattgtcga actcctttgc aaactttaga taaattcatc atatatccta tgcgagaggt
     1741 aggcaagaaa ttagaaaatt catgaaaata tttatgaatt ttacaagaaa aacataagtg
     1801 aaagttctaa ggagagagga tgcgacataa gttttgttgc atctccttgt tttgaaacag
     1861 aatttttctt aatactcgaa acaaagtagc tttttcatga cctctcggta aacggtgttc
     1921 aatcagctgc ttctcggtaa taagctcact agcctagaaa atatgagaag ctattttctg
     1981 actagcgctc ttattactca aagcataacg cagttttcac aacctcttcc ttaaacggtg
     2041 ttcaaaagct tgcatctgcg gtaataagcc caacccaaca aaaaatatga gtagctattt
     2101 ttttgggctg tacttattac ttgatgcagg acagcttttt cacaacctca tcggctttca
     2161 ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac ttgcaaggga
     2221 ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt
     2281 tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat gtggattctt
     2341 taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa
     2401 ttgaagtgca gcgaaagaaa atggaggaga aaaaccaacg attaactgac caaggattca
     2461 atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca tccagccaat
     2521 tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat atttcacttt
     2581 atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa ggaacctctt
     2641 tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt ttacctaatc
     2701 gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt ttgactgttt
     2761 tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca gatgacacgt
     2821 ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt acgccgtata
     2881 tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac gaatcggtaa
     2941 aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt attcttgcag
     3001 gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc tatcagtata
     3061 gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca gggaaaccgg
     3121 taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga aatggtgtgc
     3181 cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg cctggtggaa
     3241 cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt attagaaaag
     3301 taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa
     3361 atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc cgcacaacaa
     3421 caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat gctaacttgg
     3481 attctcatga attcattcca tcaacttatt gggagaatca agtagaatct gttaggaaag
     3541 ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt ggttttgctg
     3601 gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa
     3661 tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact ttgacagtct
     3721 atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca attcaaacac
     3781 agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat caaaacgctg
     3841 atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc tgggagcatt
     3901 atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca atctttgata
     3961 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     4021 tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttgat
     4081 aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa aatatgaaaa
     4141 gctattttct aactagcgtt cttattactc aaagcataac gctgatttca caacctcttg
     4201 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaacatgagg
     4261 aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc acaacctctc
     4321 tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg ctacggatat
     4381 catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct atacctgcat
     4441 cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa gtttcatatt
     4501 tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca gtgatttctg
     4561 gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt tcatttggca
     4621 taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg aatacgccgt
     4681 ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg accattccgc
     4741 catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat tcccaaccat
     4801 tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta aattctttca
     4861 tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt cgattactca
     4921 tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc aagaaaaaat
     4981 acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg atatgaattg
     5041 aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag tcagatttat
     5101 cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga gatcacaggt
     5161 gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca attggcagag
     5221 tggattcata cacccaccaa tgaactaccg gttaatcgag acgaatttgt tctgaaattg
     5281 tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca gcagcgtgac
     5341 cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt tcctactgaa
     5401 aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat caatcgggaa
     5461 acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa
     5521 aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg
     5581 gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct acttattaaa
     5641 aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg ttcgtagtca
     5701 gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg
     5761 atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg
     5821 tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg gtgcattttc
     5881 ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc atctaaatat
     5941 tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg acaattcgca
     6001 tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg acgtggagac
     6061 acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt ttccaacaaa
     6121 cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct tcccttaatt
     6181 atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag gactaaagta
     6241 ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca accaacccgt
     6301 agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat taattggtac
     6361 attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat tgatgaaagc
     6421 tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg
     6481 gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa atttatatct
     6541 ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac ctaattttag
     6601 tacattacta atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga tgccattatt
     6661 gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc
     6721 agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt atatcattga
     6781 taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc ttgtcttggt
     6841 tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca ttgatatttt
     6901 atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt ctagtgtagg
     6961 agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg tgattttcat
     7021 cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac ttcgtgtatt
     7081 caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata tggcgatggt
     7141 aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat cagcattcca
     7201 ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg
     7261 tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt tcattttgac
     7321 agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat atatcgtgat
     7381 cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa cgacttctgg
     7441 tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata atacattccg
     7501 tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga atgtgacaaa
     7561 agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata aagatcaggc
     7621 aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca tctttgggat
     7681 tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg
     7741 ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc tttaacgaac
     7801 gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact aatcttcgtc
     7861 gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga aaaagtaacg
     7921 gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga tatgcttttg
     7981 tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa
     8041 gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa gacagacggc
     8101 gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat gtataaattc
     8161 tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac attcaaaatc
     8221 ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga attagtgaag
     8281 aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa
     8341 gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac gaaagcctta
     8401 gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa aggcgaataa
     8461 tattttgaac accatgtagg aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca
     8521 atcagataaa aatagcactc catatttttc atctgattgg gcttagtgcc gtagatgcaa
     8581 gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactct gagtcacaag
     8641 gagcaatttc aaaaaacagc ttctcatgtt tgttgactat tgtgacttgt gaccgaggag
     8701 ttggcaggag aacaccgttt attcgaaaaa aggaactgcg acaagacttt tgtcacagtt
     8761 ccttcttttt tttgctatgc tatactagat gtaaaaaata ggagaaataa aatgagggaa
     8821 atttattctt ttgaatcaaa aatctatgct tccgaagtcg gcaagggcgg ggcatatatt
     8881 atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt ccaagcaact
     8941 tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt
     9001 tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg aaaagaaatc
     9061 ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt
     9121 cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     9181 caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg aagcggcttt
     9241 atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg
     9301 gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca taacgctgat
     9361 ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact ggttatttga
     9421 tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct tctgacatcg
     9481 gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg gaagtgatcg
     9541 tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct aagacatcgt
     9601 ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct gtatcacgag
     9661 aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg atcaataagt
     9721 ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt acttcaacta
     9781 tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt tacaatttca
     9841 aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt ttgaggctga
     9901 ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag
     9961 ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa ctttcgttgc
    10021 tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc aacagagata
    10081 cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa aaatgatccg
    10141 ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag gctttcgaat
    10201 tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg actgatgatt
    10261 ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat ggcataaatg
    10321 gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc caattgttcc
    10381 attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt atttcaaata
    10441 tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt tcatatcgaa
    10501 aggaactgta ccgtcaaaga tagtttttgc acttatccca cggaaacgaa tagagccagg
    10561 attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg ggatcacgat
    10621 gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa
    10681 aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc
    10741 aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac ggatttgcat
    10801 gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta cttttcctgg
    10861 aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg ccatagatac
    10921 gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc ggtgagtaac
    10981 tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc caggaaattc
    11041 tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat ttggcatgat
    11101 ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg ctataccatc
    11161 aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat tgtagccaag
    11221 tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta aaccaatatt
    11281 gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg
    11341 gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa ccatacgatg
    11401 gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac ccattgtgat
    11461 gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa
    11521 gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt
    11581 cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc
    11641 atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt tcacacgtgt
    11701 gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag tcggacgttt
    11761 tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt caggatcgat
    11821 ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga tgacttttct
    11881 taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta cctatctata
    11941 gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc tacaatggaa
    12001 tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa aagaaagcgc
    12061 atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc taaagcagta
    12121 aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga agtaaaatac
    12181 gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa aaaaggaaga
    12241 ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac tgaagtaacg
    12301 attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc agaagagttg
    12361 tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa aaacagcgcg
    12421 aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca gcgctcttat
    12481 gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc
    12541 ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata tagtagagac
    12601 gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta gatccgtcat
    12661 gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca cacctaatat
    12721 cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt
    12781 acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca cagatgtata
    12841 agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa aagcaataag
    12901 gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc cagctaaatc
    12961 agtataaatg attttatctt catctgattt gcctttattg taatcatcta gataatccaa
    13021 gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag gcaagctgtc
    13081 tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt cagtagttaa
    13141 gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt tttcgttgac
    13201 gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat tcattgtaga
    13261 agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat tctgatacat
    13321 cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat agtaagtatt
    13381 gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc
    13441 tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat tgccgtctac
    13501 gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag aataattgtc
    13561 ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc
    13621 tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat tttgattcga
    13681 gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc ctgtagtacg
    13741 ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat cgacgtatgt
    13801 ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt
    13861 tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac gagtctggtt
    13921 agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt gcgtgttatc
    13981 gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga tgatcccaat
    14041 actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat tgttgaagga
    14101 aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt gatcatcttt
    14161 tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa attcaatgat
    14221 tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca ctaatttttc
    14281 tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat ctaacgctcc
    14341 ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag cgatggccac
    14401 tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat ttgtcaagcc
    14461 gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa caccacttaa
    14521 tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt tgtaactttg
    14581 aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct taaagtcttt
    14641 agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc caccaatcac
    14701 attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga attctttttg
    14761 tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga ctttataata
    14821 tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa atattcggta
    14881 tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta actgaagatg
    14941 aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat gaatcgtatt
    15001 ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt ggaacgagcg
    15061 ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact agaagcgaca
    15121 cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta tgacttgcta
    15181 gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc aaaggccgat
    15241 tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac aaaacccatc
    15301 gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa gatcaattac
    15361 agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac agtgttacaa
    15421 gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa attactgtct
    15481 tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg attagatacg
    15541 aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg ttttgaaaag
    15601 acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt tatttaaaga
    15661 tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc ttcatttcga
    15721 tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga cattctatct
    15781 cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt gcggggttca
    15841 gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg catccaatca
    15901 gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt cgaatggatg
    15961 aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt atggtccaag
    16021 aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc catgaattca
    16081 aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca aatctctctg
    16141 acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag ctttcacaat
    16201 taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga
    16261 aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag cctaaatggg
    16321 agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca ggcaatgaag
    16381 agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac aatcaaatca
    16441 atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg gaagtgaccg
    16501 attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa ttttatcaag
    16561 gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt aaaaaaattc
    16621 tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga acgacagcaa
    16681 tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc tattgaaggt
    16741 ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg ttcttttggc
    16801 ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca ttgaaacaag
    16861 gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg ggtggataga
    16921 tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa aatgtatctg
    16981 aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct gttgttggag
    17041 aaatagggag aatcacagta ttttctttta atagattctt gactgttttt tccataacaa
    17101 tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt gctcctttat
    17161 aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt tgatacaagt
    17221 tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga tccaatttct
    17281 ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct gtttcagtag
    17341 gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac
    17401 tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc aactggactg
    17461 taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta taattgatca
    17521 ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag gtttcaagca
    17581 cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc aataagcttc
    17641 cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg tctaattctg
    17701 tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt cgatcagcag
    17761 ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta tggatatcca
    17821 tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg aattggtgta
    17881 atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag aaatttttct
    17941 gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg agttttgatc
    18001 aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa taaattaagt
    18061 gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg gatgggaatc
    18121 tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt gataggaaca
    18181 gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc gctgatgcaa
    18241 gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga tgaatttatc
    18301 agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat ctatggaagt
    18361 ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt ccttgatttt
    18421 ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat agcaattccc
    18481 caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc attgactaca
    18541 ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc taccggtttt
    18601 agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat gatcgttatc
    18661 atgccactga gctggatgtg gacaaattgg attatgccac tgttataata aaaatcaaag
    18721 agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt tttgtatttg
    18781 acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat aaaatattac
    18841 acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt cttgatggta
    18901 ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca aaaatcgtac
    18961 aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga ttgaaaaaag
    19021 aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga tattactcgt
    19081 tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt gtttttcgag
    19141 attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa tgaagttgtg
    19201 gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta ttatgaggaa
    19261 tattttttag attctggaaa aacattagat cagatcattg atgaatatgg tacaccaaag
    19321 cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc agaaaacgag
    19381 ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat cggtttattt
    19441 gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg gggactattg
    19501 gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat cttaggtgtt
    19561 ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct gagtggatta
    19621 ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc gccactgttg
    19681 ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg gatgggacga
    19741 cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac acataaattg
    19801 agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg cctcctagca
    19861 ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg tcctcggttg
    19921 atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca
    19981 gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac gacttatgat
    20041 aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa
    20101 aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac tattgttgtc
    20161 cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc aattgatatc
    20221 aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt atcgttcaat
    20281 gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg attagatgag
    20341 gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct agaagacagt
    20401 ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt gcatgagatg
    20461 gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa agactaccag
    20521 ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac tgctaaagac
    20581 acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc ctgtactcaa
    20641 ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt ttcatcgctt
    20701 aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac agggtgaaag
    20761 agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg gaaagattac
    20821 atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa aaagtgatgg
    20881 ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat gaagttttca
    20941 aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt atcgatacag
    21001 gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt cgggaagatt
    21061 tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt acagaagaaa
    21121 agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg acacaggcaa
    21181 gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg gcgcgtattc
    21241 gaaaaaaaat taaaaaagta acattagata tttattgaag gataaaggag cgcaggaagt
    21301 agcatgagag aaaaaagagc attttatatc aatggttatg cagggattgt cggattggtc
    21361 gtattagctt tagttggatt gtttttcttc tatttaggta tgtggcaagc gaaagtttgg
    21421 gcactttttt taagtatttt tctttggttg atcacgttat tgttgttaag ttcggcaact
    21481 gtagtcagtc ctaatcaagc aaaagtaatt ttgttttttg gacaatattt aggtacgatt
    21541 cgagaaaatg gctttttcct tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa
    21601 gttcggaatt tcaatagttc tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa
    21661 atctcagcag tagtagtctt taaagtcgtt gatacagcaa aggcattatt cgatgtagcg
    21721 tattatcaag attttgtaga aatccaaagt gagacagcca ttcggcatat cgctagccaa
    21781 tacccgtatg atacattcaa tgagaatgat ttgacgcttc gaggaaacac gacggcagtg
    21841 tctgacgagt tgcaaaaaga gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc
    21901 gaaacacgct tgaaccattt agcctatgca acagaaatcg ctagtgccat gctccaacgt
    21961 caacaagcaa aagccatttt gtctgctcga caaaccattg ttgaaggagc tgtcacaatc
    22021 actcaaatgg ccttagagca aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg
    22081 aaagtccaat tgatcaataa tttacttgtc tcaatcatta cagacaaagg aacgcaacct
    22141 gttatcaata caggcgacat gaaagaccgt aagtgacata ctttttgtaa gataggaaga
    22201 aggagtttag gacggattat tgtcttgaac tcttttttta gttgttttaa agaaaatgac
    22261 aaaattgaaa gaagaatgat agttgattcg aaggcagaat tgttcgcttt tttctataat
    22321 cagtatataa agtaagagag aaggggaaaa agtgaaaaaa cttctgatcg gtctacttgt
    22381 tttcatcgtt gtggtacttg caggggtgaa aatcgcagat tacgtagtta tgggtggaga
    22441 agaatactat gtacaaatta cgacagatgg tacaaaaaca gtttcaaaat cagactctgg
    22501 acaagaaaca gtcaactata gatatatcct gcctggctat acgaaagagg gtaaggaaaa
    22561 agaactggac ttcaagggac aattgactcg accgcttcgt aaaggagcat atttaaaaat
    22621 cacctggaac aaaaataaag gtgtcacttc gtatgaagaa gtcgaaaaaa aagacgtgcc
    22681 gaaaacagca ctggaaaaac taattaaagg gtagggaaag aaatgaacaa aatagtcgaa
    22741 gtacagaatg taacaaaagt atacggaaaa aacaatgaga aaaagacgca agcattaagc
    22801 ggcatcagct tcgatgtaga aaaaggcgaa tttattggga tcatgggcgc ttctggttct
    22861 ggtaagtcga cacttttgaa tattttatcc acattagata aaccaacaga tgggcatatc
    22921 cgaatcaatc aaaaagatgt gaccacatta aaaggaaatc aactagcaga ttttcgtgcg
    22981 aatgagattg gctttatttt ccaagatttc aatttattag aaagcttgac agcacaagaa
    23041 aatatcgctg ttcctctttc tttacaaggc gtcagaccaa aagaaatcaa gcaacgtgtc
    23101 caaaaaattg cagaacgttt atccatctct catattttgg aaagctatcc ttctgagatt
    23161 tccggtggac aaaaacaacg ggtcgccgcc gcacgtgcat tgatcacaca accaacgatt
    23221 ctattagggg atgagcctac aggtgcgctg gattccaaaa gtgcccggga tcttttagat
    23281 acgatggacg aattgaatac aaaagatcat gtatcgattc ttcttgtgac gcatgatccg
    23341 ttttctgcaa gttattgcca acgtatttta ttcatcaagg atggcggtat ccatcaagaa
    23401 gtgaaacgtg gagatcaaag ccgtgattcc ttctatcgag aaatactatc tattcttgga
    23461 aacttggaac agtaggggga gattgactat gctttggaaa ttatcactta ctgggatcaa
    23521 gagccgttta agagactact tggtgttatt ctcaggatta gttatggcat ctgctatttt
    23581 ttacatgttt gaatcaatgg caagcaacga agcttttttg aaaagtaata cgatcgttgc
    23641 ggctacagtt atcattttcc gattcggttc tgttttacta gggatcatca cattcgttta
    23701 tattttatat gcaaattcct ttttgatgac gatgcggcaa aaagactatg caatgtttat
    23761 gatgctggga gcaaaaggca gaaaaatcgc acagatgatt ttttctgaaa cattcttagt
    23821 agggatcgca gctactttag tgggttcagc tgtcggtgta ggattgacaa gtatcgtcca
    23881 ccgattatta gtggatcagt tagatctcaa tatttcgcat tttacacctt ttagtattca
    23941 cgggttgttg atcactgttt tgttttttgc tgttttgttt ttattagctg ctgttgtcaa
    24001 tgctttttcg attgtcaaaa aaccgatttt agcattgatt cgtgcggatc agacaccaac
    24061 aagaatcaag cagcataaat tcttgttttt ccttgaagta gtactgggaa ttgcttttct
    24121 aggctgcggt tactatatga tgaaacacgt ggggacctat caagtctttg gtataggggt
    24181 agccttagtt acgatcgtat taggaactta tttcattttc cattctgtga tcattttctt
    24241 tttatcatta ctaaagaaat cagaacagat ttcattgaaa aaattgaaca atttcacatt
    24301 gtcacagtta aactttcgta ttcgtgaata tacacaaatg ctctcaatgg tcgctatgct
    24361 gtttgccttg gcactgggcg ctttgacggt aggattaggt tttaaaaatg aaattgcaga
    24421 cttgacagaa aacaattctg gatatgatct agtgatgaat aatcctcggg cagaagatca
    24481 gcaaaaaatc aaagaacttt caccaacact ttctgcttct tatactcaaa aagaagatgc
    24541 gggaacgatt tactataatg caagtgaatt tgcggctcaa ccattgatca tgataaaaca
    24601 tgaagatgga acaccgccga agatcacgaa agaaaaagtt cctgctaaaa aaatgaatga
    24661 actgactgta caagaggaac ttcgaagcta tgaattgccg gaacagcaag aaaaagaaat
    24721 caaggtagtc agtcaatcag aatttgatca actgaacttg ccacaatcca ctttacagct
    24781 agttcaggta aaagacttcc aagcaaacct gaaaccaatc gaaacacttg ttgctcaaaa
    24841 taaaatgaac aatccatctt tgcaaaaagt agagtacagc aaccagaaat atgaaatgta
    24901 tgaagtgtat aatggtctat tctctggttt tgaatttatg ggattcttcc taggtttagc
    24961 tttcttgacc atgcttgcta gctgtttgat gttcaagatc ttgtcaggaa gcaagagtga
    25021 cattgctcgt tatgaaatgc ttgaaaagat aggaacacga cgcgggttgc ttaaacaatc
    25081 gatccgtaga gaagtaggcg tgttattctt agcaccagga atcttaggtg tagtccatgt
    25141 cttattcgga ttacaaatgt ttggtctatt catgcaaaat ccatacaaag atatttgggt
    25201 tccgtttacg atattcttcg tattgtattt catctactat gtactgacga catggttata
    25261 tactggaatc gtattgaaaa agagattgtg aaaaagctgt cctgcatcaa gtaataagaa
    25321 cagccaagca aaaatagctc ctcatatttt tcgtttggtt gggcttatta ccgcagatgc
    25381 aagcttttga acaccgtttg ttcggaaaaa gaggttgtga caatattttt gtcacaacct
    25441 ttttttcacg gtcttttatt agaatatagt aaattttttc tcgttactca cggaataagc
    25501 tgttaagaaa acggtttcca tgtatactgg aatcagtgca aatgaaagaa aagagggtgc
    25561 aaggaacatg gaaatcaatg aacaaaattt tggcgaaaac tatcacttga ttacgatcac
    25621 gaatcaaaat gggacatctt tgtctgtcag tgatcttggg gcacgggttg ttcattttca
    25681 aacaataatc gataatgaaa aacgagagtt gatcttaggt tttgatgaag caaaagaata
    25741 tctagaaaaa gacccttata tcggtgcttc aatcggacgt atggctggac ggattacgaa
    25801 tggcacattc aaattagatg atgaaacgtt cgaagtaaaa acagacccgg acactggtca
    25861 ttgtttacat ggtggtgcac ctagctttga agccaagcag tggcattata cagtcatcaa
    25921 tggtgaaaac gaagcatcgg tcattttcta tcagactagc ccagatatgg aaaacggatt
    25981 tcctggaact ttagatgtag aagtacggta tacgctgacg aacgatgata catggcgagt
    26041 gacggtccaa gggatcagtg accgcaaaac agtattcaat cctacaaatc atgtctattt
    26101 caatttaacc ggtgatccag cacagtcaat cgacgaacat atactttggt tgaatagccg
    26161 attatttgct acgctcaata aagatacaac gcctacagga gaagtagttt cagtaagtgg
    26221 cacagcattt gacttccaac gtcctaaaaa attaggtgag gtctttgcca gtgattttcg
    26281 ccaatcaatg atagtgggcg gcatcgatca tccatttttg ttgaaaaaac aatctggttt
    26341 actaaatgct gcggaattga caagcccaga tgaaaaagta tcgatccaag taaaaacaga
    26401 tgcttcagga atcgtgattt ttacagcaaa ttttggagaa gatggaccgg aaatgcgcgg
    26461 gaataaatta gtaaatcatg gcggtatcac atttgagact caagaactgc caggagcgga
    26521 gcgacttgaa agttttggag atgtcactct ggaacctaac caagtacgcg agtttgtaac
    26581 ggaatacaaa ataaatataa agaaatagaa taaattaaaa agggagtgtg acagtgtcac
    26641 atcccctttt taatttattc tatttcttta aaggcagatc aagccagaat gatttgtcta
    26701 aacttaacaa ggttagtaca ggaacagttg cagctttaac tgtacgagta gttgtttctg
    26761 gtatctgtct tgccgtaaaa ttgttccaca aatcccagat tgaagcgaca cggaagatgg
    26821 aagaagcgtt gttgacgcgt tttatcgttg aaaattctaa tcctgtgatt ggcacgccat
    26881 ctgttgtcca tttgattggc acatcgaatc gcttagacat attgaggatt tcagcgtttg
    26941 cttcttcagt tgttgctgct tcttttttcg gttcatcata ttttgtcagg tcgtacgctt
    27001 cgatgatcgc gttcaatttt tgtgaatatt ggcgatctgt agcataacgt ccttccaaat
    27061 atttggttgc ttctttgtag tcttttgttt ccgacttcca agttggtttg tagaaatcag
    27121 gattgccagt cagtccattt ttgatcaagt cggcatagtc ttccaaactt tccttgtaag
    27181 aaggatattt acggaattgt gcgcggattt ggtagctttg tccggaacta tcttgttcaa
    27241 gtgtattgaa ggtcacgctg cttcctttat atgatccttt gataccaaaa aggttatgat
    27301 ttggttcgct tgataataag ctgtttccag atccactttc aaggatagct tgagccatca
    27361 tgactgatgc gtagattcct tctttttgcc cgatttcctg tgcatcatct gcaatctttt
    27421 tcaagaactg atcagttgtt tggttttgac tgaaatggaa cgagccatct aatccatcta
    27481 aaatagatga gccgcctgtc agcgaacctg aagcagcagg taaacctgct aagtttactc
    27541 ggacagcaaa atcaggcgtg aaatctgaaa tagaagttac tcgaacttta tctcctggtt
    27601 gaggcgcatg gataaattgt ccgttcccaa tatagatacc tacatggtga gtaacaccgg
    27661 aagaaggtcc ccagaagacg aggtcgccag gttgggcctg acttactgga atctgcgtac
    27721 ctgcatattg ctgttcgcct gtccagccac cgatataatg accggttgct ttcatatata
    27781 cgtaataagt caaaccagaa cagtcaaaag tatttggacc ttttgctccc catacatatg
    27841 gttttccaag atgcttgaat gcttctcgaa cgattgcttg ttggacattc gttgtatttg
    27901 taggcaatac agcatcaatt ggtgatttta caacaaaatc agaagaagca gaagatggat
    27961 tctctatacc gctttgattt gctgtatttg gacgagatga agcattgttc gttccgccat
    28021 tcgatgaacc attgtttgga tggtttggtt ctctctgagt atttccattg ttattcgatc
    28081 cagatgggtt tgtttgattc gaagaattcc cgctgttcga attattgtta ttattgtttg
    28141 aattatttga accattatta ttgttgttat tacccacatc tggtttgttt ggcgtttccg
    28201 gatctggtgt actagggact tcttcactag gatcagttgg tttctctgtt gaagtatccg
    28261 gtccagcttt aaattctgca gtaatagata aattgttcgt acctactgta aaggtctttg
    28321 catcataatt tacttctttg gcaccattaa cagcgactga agcaatttgt tcaccttcag
    28381 ctggcgtaat agtgtaagtt accttcgttt ccgctaataa accttctacc gttccacttt
    28441 ccgtaatatc caaaggttgc tgttcagggg aatccgctgt cgaagcagtc agttggatct
    28501 taccaatcaa agaaggagca attgtaaggg aatatgtctc ggctggcttt tcattttctg
    28561 atggatcagc cggatcaatt tcagttggct cggtcgatcc tgttgttgag ttagttgttc
    28621 cagttgggtc ttctgtaaaa gaatccgttg tttcactagt agctgagctg gatgtagtcg
    28681 tatctgttgt gactgtttga tcagctattt gattatctac ggcaaatacg gtattggtcg
    28741 acaaaacaga cggagcaatc agccaagtag cagctagcaa acgtatcgtt tttttatttt
    28801 tcataataaa ctagtccttt tcctttcatt gattttctgt cttattcagc attcattaga
    28861 cattttttgg aacagataat cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt
    28921 attctaaata aaacaatttg taaataagca acgagcattc attcgttttt ctttgacgtt
    28981 cattttattg catagaccac ctttgtttgg ggataaacaa gtggggataa aaatggttat
    29041 gcatatgtaa attataccaa agtttggaaa gtatcacaaa aatttataaa aataatgatc
    29101 ctaaaaagaa aaagactgaa atatgtgata aacatctcat acttcagtct ttttcattaa
    29161 ctattcataa gacattaggt cttcatcaaa cccgctaacg agagtagtga tcattgctac
    29221 gactagacta acagccagtc ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg
    29281 caatgtggtc agactaccaa atacaaaagc gatcgctttc acttgggcaa atcccattac
    29341 agctccacca gcagctgcac caatgcaagc agcaacaaat ggttttttca atgggatcgc
    29401 aattccgtaa acagttggtt cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc
    29461 ggagatcgaa cgggtttttg aacgtttact tcgcagaaat acacctaaag ctgcaccagc
    29521 cattccaaaa ttggctgctc ctgtcaacgg aacgattcgg tcgaacccat aagcagagat
    29581 attactgatc atgacagggt ttacagccca ttggatacca aatattacca aaatattcca
    29641 accaccagcg atgattgcgc cactgatcgg tccgttaata tccatcatcc aagtaattgc
    29701 ggcagctaaa ccttcgccaa ggtaaacacc cgcaggcccg aataccatta acgtcaaagg
    29761 aaccgtgata actaatgaaa gtaaaggtac aaatactaat tgcatatttt cgcgaatata
    29821 ttttttgagg aatttttcaa caaaagaata aaagaaaata cctaaaatcg ccggcatgac
    29881 agtagacgta tactgcatca gtactacagg aatccccaga aaatcagtag ctgctttgcc
    29941 actgtctgaa agtaaagagg tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc
    30001 aatatatcca ttcactttaa acgttttggc agcagaaaaa gcaagaaaga ttggcagaaa
    30061 ataaaagacg gcattcgacg cagcagacaa aatatgataa gttccgcttg tttcactaag
    30121 ccagccaatc gtagtcgcta aaacaaccag accttttaat ataccagaac cggcaaacag
    30181 cggaatgatc ggcgtaaaga tttccgatat caaaccaaat agtttttcga tcggatttag
    30241 cttttctttc ggagctgaat gatcgatttg acgcgtcaat tccttgtaag caggagccaa
    30301 tccctgatcc atgattattt ctgcatatct atcgagtaaa atgaaatact taatggaagg
    30361 gatttgctgt tttaattttt ctgcattggc tactggctgt tttttcaatt tgatacgaag
    30421 ccgagtcgca catggaagga cagaatcaat attgtctttt cctcccaact gttcaatcag
    30481 catatgaatc tgctcggaca gtaccatctg ttcaggattc attgtcgttt ccattagaag
    30541 accactccac tcactaatat aatattggcg taaggtgtca tttcgcctgt acgaataaat
    30601 gcatgacagt tattcaactc ttttttcatt tcttcatgtg ggataaagct aatcggtgta
    30661 tctggtaaca ataatttgat tttttccaat atttccgggt tcattgtctt tatttcttcc
    30721 gccaattcaa tcgattgtac ctcaagttct tctaaaacgt tttctaaaac agataaaaaa
    30781 cttggaagac cgtttgagac agccaaatct attttttcag taccaaaggg gacaggcatg
    30841 cctgcatccc cgatacttaa tttatcaaag tgccccattt gtgcgattac tcgtgaaata
    30901 tctgaattga ttacttttcc ttttttcatg tttgtttctt cctctcattg ttaattttag
    30961 actgctgatt gttctaaagc ttcgcggtaa gggatagaag gttgcgcacc ataagactgc
    31021 acagcaacag aagaggctag atttccataa aggatcgctt ctttcaagtt actaaggtct
    31081 ggctgaagtg tgctgcttaa tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg
    31141 gcatccactt taaaagcagg gacgataccg tgttcttttt ctgtatgata gaaagcgccg
    31201 gcactgccta gtgtaatgat tactgttcca atacctaatt catggagttt ttcggctgct
    31261 gcgaccaaac tgttatgatc tgtcactctg attcctgtaa tgatttctgt ttctgtttca
    31321 tttggtataa tgatatctgt cttttcaagt agttcagtag gaatcgtttc acgagctggt
    31381 gcaggattca ggatcgttgt ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc
    31441 gcatctaagc tactttcaaa ctgagcaata ataaagtcac tgtttgtaat cagttgcttg
    31501 ttttttagga cttcttgttc atggaaagca ttatttgccc ctgcatggat caagatacta
    31561 ttttctcctg ctgcgtctac cataatcatt gcttgtccgg ttgtggcgcc ttggatttca
    31621 gctatcccag atgtatcgat gttttctttt gtcagtagat ctaataactg ttgtccttcg
    31681 ctgtcagccc caacgcctcc aataaaagat gtccgtgcgc ctgaacgttt ggctgccact
    31741 gcttggttgg cacctttacc tccgccagcg tgaaaaatct catgcgcatg catcgtttcg
    31801 cctggcttgg gcatgttggt taaacgaagg gtagtgtcca tattaatact tccaattact
    31861 gtaaccgtgt tcattttaca aacctccaac tatctcattt attttgatgg gaaaattatt
    31921 ttttaaaacg ttttagtaaa acgttttaat agttcgataa aaatatagca caccttgaaa
    31981 gctatttcaa gggtgcagtt gaaaatcttt tttctaatcg tacaggtaat cttttttctt
    32041 cccattcttt ttctgggaat tggatccgat ctaacaataa cttggcagat gtttgtccca
    32101 attcgaagat tggttgtgcg atcgttgtca gtttcggctt gatatactcg cacatatcta
    32161 tattatcata gccaataatg ctgtagtctt cagggatgct ttttccagct tcctctaatc
    32221 cgcgatataa tccaaaagct aattcatcat ttaaagcaaa cacaccagtt gcagcagaat
    32281 caagaagttc tgctgtgatc tggtagccac cttgttttga gaactgtgta ggtaagagga
    32341 tcagttggtc gtgagggatt tggtaaacat caagagcaga tttaaacccc tcaatccgag
    32401 cgtggacatt ttccggtgga ttttcaggat agacaagggc gatcgtttga tgtcctagac
    32461 taagcaagtg catgcccgct aggtaaccac cgcgaaaatc gtctgttcgt acggcatcac
    32521 taaatccttc agattttttc tgatccaaaa caatgaaggg ccggccttgt tttttcaaat
    32581 tttcgttgat tgcatctgta gagacagcag aagtcgcgat aatgaatcca tccacacctc
    32641 gacgtgtgag ctcggctaga tattcgattt ctttttgatg atcagaatca gcattacata
    32701 atatcgtgac gaaattctgc ttatacaaga tatcctcaat tcctcgcatc agtgtactga
    32761 agaatggatt cgtgatatca ggaacgagta caccaattgt tttcgtttcc ttggtaatca
    32821 tttgtctagc aaaatagtca ggctgatagc ctaattcttc tttggcagct aaaactttct
    32881 gaacagtttt cggactgaat ttctccgtat ttccatttaa tatcaaggag acagttgtga
    32941 ttgaaacgcc ggaaagttta gcgacttctt tgattgtgat cttttttttc accatgttca
    33001 tactcctatc taaatcctgt ggttattttc ccatgaaatc aaaaagaagt aaatgaaaat
    33061 cacgtgtgcc agtacttatt tgctttgttg gatagcttcg attatttgtt tcgttgagcg
    33121 tactcgtgca gttttcggaa atatcttttt tatgaacaat tgatgggatt ctttgtcacg
    33181 atcactgcaa gcatcttcag caagcacgat atggtacccg tgttgaaagg catctaatgc
    33241 agtagcatat acaccgtttg ttgtcgctac accgcttaaa atgatagttt cgattccacg
    33301 ccggcgaagt tgaagatcaa gagaagtacc aaaaaaagca cttggattgt attttgtgat
    33361 agtcaacata ttatctgtat ctttcaaaga atccttcaaa agtaaatcag tgaattctgg
    33421 aggtacagaa gcagtcattc cacccatatc agtttgttga ctcaaataag ggaaagtgga
    33481 gatgtctacg ttcactaaaa ctttcaaggc agcggtacgt ttcaatgttc ttactaacaa
    33541 atcattgttt gtcagaatag atgctgcagt gtgtggagag agtgtaccgt tatttacgat
    33601 ctctttttgc atgtcgatcg cgacaaaagc agtttttgat aaatcaaaaa tcatattttc
    33661 ctttaaatca ttcatgatgt tccttctttc aaataacaat gttttttatg taagtaaatc
    33721 ataaatcgtt tatttgtcaa ctaaattaaa ggattccttt ctttagtcta gagaaattca
    33781 gtggtaaaat aaataagtgt gagaaaacgc attcgatgca tagatatagc gagaaatatc
    33841 gtgaaaatgg aggaatcaga gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca
    33901 aaatgggaga aacgaccatt acagcaaatg acgatatttc tttttcaatt gaaaaaggcg
    33961 aattagtgat tatacttggt ccaagtggag caggaaagtc aacgattctt aatattttag
    34021 gtgggatgga tacacctgat gaaggtcaga tcattattga tgacacagat attgcgcaat
    34081 tttcagataa acagcttact gcttaccgac gaacagatgt agggttcgtt ttccaattct
    34141 acaatctcgt tcctaatcta acagcaaaag aaaatgtgga attggcaacg gaagtttctc
    34201 ctgatgcgct tgatcctgta gaggtattga ggcaagtggg cctagctcat cgattgaata
    34261 actttccgtc ccaattatca ggaggcgaac agcagagggt ttcaattgct cgtgcactag
    34321 ctaaaaatcc caaacttctt ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg
    34381 gcaagcaagt attgaaatta ttgcaaaacg ctagcagaca acacggcaat accgttttga
    34441 tcattacaca taattctgct ttagcgccca tcgctgatag agtcattcac attaatgatg
    34501 ctaaagtaag gtctgttgaa ttaaatgatc atccttcatc cattgatgag attgtatggt
    34561 agggggattt tatgaaaaca agaacatatc ttaaagcagg ttttcgcgaa attcggcaat
    34621 ctaaaggacg attcattgca atcatcctta ttgtcatgct aggaacactt ttatttgtag
    34681 gtgtcaaaac tgctggacct gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag
    34741 gactttctga tttgcaaatc gtttctactg gtggcttgac acaaaaagat attgctcaag
    34801 cagaaaagat cccagatgca aaagtagaaa caggaaaaca gatctattat tccaatactg
    34861 gcaaaaacga agtgattcaa atattttctt ataacaaaaa taacaagcag aacaagctac
    34921 aattaactga tggaaaactc ccggaaaaac caaaccagct tgtattggat gaaaaagcgg
    34981 aagatgaagg gtataaaatc ggtgatattt atcagattga ttcagatgaa ttgaaagaaa
    35041 aagagtatac catcacgggc tttgtccgtt ctccattgtt catcaacaat ttggagagag
    35101 gatacgcgaa cgtcggaaat ggtagcgtcg attattttgt ctatttacct gaatccgttt
    35161 ttaaaacaga tatttattct gtcttatatc ttgattttac aaacgtaaag aatttgaata
    35221 cgtacagcaa agcttacaaa gataaaatgg agcaaaatca agaaaaggca gaaaagtatt
    35281 tacaagatcg acccgaagaa cgcttagaag agctgaaaaa atcggccaac gaagaattag
    35341 aaccggcaca gcaaaaaata gcggatggca aagcacaaac aacgcaagca agaacacggt
    35401 tagaagatgc taaaaaacag ttagcgcaac agtcagcaac tatccagcag cttcctgaag
    35461 cgcaacgaac aatcgctgtt caaacattat ctcaacaaga agctcaactt gctgaacaag
    35521 aaaggcaatt agttgagaaa gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa
    35581 atgcagaaaa aattgaaaat ttaactaaac catcatacct atacaatgaa cgttcagaaa
    35641 atgtcggttt tcaagaattt ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt
    35701 ttcctgtttt ctttttcttt attgccgcat tgatcacctt tacgacaatg acacgtatgg
    35761 tagaagaaaa ccgcagggaa atcgggacat taaaggcgtt aggttatacg aaagttgaaa
    35821 tcgcaaaaaa atatgcaatc tatgcaagtc tagcctcagg gataggtatt gttttaggaa
    35881 cgatattagg aacgaatctt ttgccaagga tcatttttga gctgtctaac gagcggtatg
    35941 atattgggag tgcgcttatt ttttatgact ggccgccgat tttacaagca gctgccgcat
    36001 tcttcattgc cgcatttggc gctgcaatga tcgttttatt caaagattta agagaacggc
    36061 ctgctgcatt gcttcaaccg aaagcaccga agccagggaa acgcattttt cttgaataca
    36121 tcactccgct gtggtcacgc cttagcttta atcaaaaaat cagttaccga aatctcttcc
    36181 gatataagtc aagaatgctt atggcgatta tcggaattgc tggatgtgcc gggctgatgg
    36241 ttgcaggcgt gggattgaaa gattctctaa gttctgtttc agccaaacaa tttggaccga
    36301 tcattgacta tcaagcaatc gttacattgg attcatccac ggaagatagt cgtcaaaaag
    36361 cagcggatat gctgcagaag caagaaaaag taaatgagat cctagctatt catactcaaa
    36421 ccgtagaatt gagaaaaaaa ggacatgctt cgcaaagtct gacagtgatc gtaccaagta
    36481 aaacagctga tttggatcgt tttatccatt taactgatct tgaagagaac ccacgctcct
    36541 tgcctaaaac aggaatcgct attacgcaaa aagtagcaga actatttgac ttatctacag
    36601 gagatcatat ttcgctttat gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa
    36661 tcgtgaaaaa ctatctaggt agctttattt atatgtcccc tgaatattat gcttcagtta
    36721 ctgatcaatc gatgatggat aatgcatttt tgctgcgtac agataagatg acaacaaaag
    36781 aagaagaaat gctttccgag aaactgcttg ccacagacgc tgtgacaaat acgtctttca
    36841 tgtccaaaca aatcgaaacg caagaagaat caatggcgaa cttagactcg gttgtcttaa
    36901 tctttgtcgt attatcagga ctgctggctt ttatcgtctt gtataatcta actaatatca
    36961 atatttccga gcgtgtacgc gaattatcta caataaaagt ccttggattt tttgataaag
    37021 aagtcacgat gtacattgtt cgagaaaata tcatctttac tcttttagga atcatcggag
    37081 gatttggtgt aggatatata ttgactgact tcatcttgca acaagcgtct atggagaatg
    37141 tcatctttcc attagtgatc acatggtggg cttatgtctt atcagcagga ttaactatcg
    37201 tatttacggt tattgtgatg attgttaccc atttcaaatt aaagcatatc gatatgattg
    37261 atgcgttgaa atcgaatgaa taaaaacaga ggttgtgaaa tcagcggttt gacctgagta
    37321 ttagagaaac aaacggcaaa atagtttttc atattttgcc gttttttgag ctaataccga
    37381 aggacagctg attgaacacc atgaatcaga ggttgtaaaa aagccgtcct gcatcgagca
    37441 aaaagaatac tcaaataaaa ttagctcctc atgttttaca tttgagaaga ctgattgccg
    37501 tagataaaag cttttgaaca ccatttatag aagaaacaag aatattttgc tattttaaac
    37561 aagaggatag acaaatacat gtctgatctc ttgtttttta ttgggcaaca gacctcttag
    37621 aatgtataaa gataaatttt acaaacgctt tcggttcagt tataataata aatgataact
    37681 tgtatatata agttgtggta ggtaataaat cagttgaatg gaggtaaata tccatgacat
    37741 ttaaagacaa agtcatttac caaatctatc ccaaatctta caaagacagt aatggtgatg
    37801 gaattggaga tttaaaagga atcaaagaaa aactcccgta tctgaatgat ctaggcatcg
    37861 acatgatttg gctaaaccct atttatccaa gccctcaaaa agacaacggt tatgatatct
    37921 ctgattacac agccatcgat cctatctttg gtacgatgga ggaattcgaa cagctaatag
    37981 ccgaagcaaa gaaataccgg atagaaatca tgctcgatat ggtgctgaat cacgtttcga
    38041 cagaacatga atggttcaaa aaagcactag caggggacca gcattatcag gacttcttca
    38101 ttttaagaga agagccaacg gattgggtat caaaatttgg tggaagtgct tggtcgccat
    38161 ttggagatac ggggaaatat tatttgcact tatatgatcg gacacaagct gacttgaact
    38221 ggcggaacca agcagtaaga gaggaactat tcaaagttgt tcgtttttgg atggaaaaag
    38281 gggtaaaagg attccgtttc gatgttatca atgttatcgg taaagacgaa caattgaaaa
    38341 ataataaaga aaatgatggc aaacctgaat atacggacaa accaatcacc catacgtatc
    38401 taaaagaatt gaaccaagca acgtttggga cggattccac tatcatcaca gtcggtgaaa
    38461 tgagctctac gacgatcgaa aattgtatct tgtatacaga acctagtaga gaagaattat
    38521 caatggtctt taatttccat catttgaaag tcgattatga aaatgggaat aaatggtcta
    38581 ctcctccatt tgattttgca gaattaaagc gactatttca tacatgggga gaaaagatga
    38641 gtgagggtaa cggctggaat gctctatttt ttaataatca tgatcagcca cgtgcgctca
    38701 atcgatttgt agctatcgat cagtatcgaa aacaaggagc agagatgcta gcaaccatga
    38761 tccatttgaa ccgtgggacg ccctatattt atatgggaga agaaatcggc atgatcgacc
    38821 cagactattc atcgatttcc gactatattg acgtagaaag tttgaatgct tataagttac
    38881 tccttgaaca aggattcaca gaaaaagaag cgtttcgtcg gatcaaagca aaatctcgcg
    38941 ataactctcg aacacccatg caatggtcag acacagaaca agcaggattt actacaggga
    39001 aaccttggtt aaaagttgga ggcaagctag aagagattaa tgtagaaaaa gaactatctt
    39061 ctgaagattc tattttgtcc tattacaaga aattgattcg gctgagaaaa acatatccaa
    39121 tcgttgcaca aggagattat catgcttatg aagcaaatca tccggaagtt tatggctttc
    39181 ttcgccaatt tgaaggacag cagctgcttg tattaacaaa tttctatgat aaagatacaa
    39241 ggatcgccat cccagaagga ttcacagaag ccaatattct tatcgataat tattccgata
    39301 ctgttcttca tcaaagtctg gagctaaaac cataccaggc aattgctttg ctgaaaaatt
    39361 aaaacaaaaa cacaaaacgt cgttcctacg agctattctc gtaagatcga cgttttgtgt
    39421 tttatttaaa ataatcttct tcagaaaatg aagagttttc atactctcct ttatcgccat
    39481 aaaatttatt gtagcgttgt ttgaatttac ggaaaataaa agtaacgagt aatttgacta
    39541 acgtatagat aggaatacca aaaataacgc ccacaattcc cattagatca cccatgacaa
    39601 gtaaaacgat taagatcgta atcggatgga tctgtaaacg atcacccatt actcgaggaa
    39661 tcaccaagtc accgtggaga agttgtacgg caaaccagac aatgacaaat ttgacagcca
    39721 taaaagtaga gtcttggaat gcaatgatca aacctggaaa gaaggcgata aacggcccaa
    39781 tatatggaat gatacataac aacccagcag aaatagctaa aacactagca aattctaatc
    39841 cgatcagtaa gaaacaaacc cagtagactg ctccaaggat aaatgaagca atgatttgcc
    39901 cctttagaaa tgaacccaat tgttgattag caatctttgt caaattttta aaatctgtac
    39961 gaaatgctgg tgggacaatc gctaaaactg actgataaaa tttttgagga tctctcaaaa
    40021 ggaaaaaagc gacaattgga ccagtaaata aagtaataaa aatagctgta acagttgaaa
    40081 aaatgttgct gatccgttgt gcgccacttt cccaataatt tccaataaat ccagctatat
    40141 cagacgtcac gtcgtcaata gaagcgaaaa attgattaaa tgaatcggca aaaggtgttt
    40201 tctccaaaaa ttgtcggaga ctctcttgaa aatcttgaaa caaatctggc aaactactga
    40261 tcaatgtctt tgcctgttct tcaagaaaag gaataagcca tatgcttcca agccccaata
    40321 aaaataaaat aatcacataa acgataatga ctgacaagtt cctcgaaagg tgcttgctca
    40381 aacgtttgat gactggttcc aaaagataat acaagatcag tgcaaacaat aatgggggca
    40441 agatagtagc gataatgatt ccgacaggct tgaatataaa cgagatctga tggaaaaaat
    40501 aaatgatcag cgcaagtaag ataactattc ctaaaacata gtaagaggtt tttcctccta
    40561 aaaagcggat caaacgattc gttgtatctg tttctttttt cactcttttt cctcctttat
    40621 ctataacttc attatagaca aaagcgggta taaaaggaaa atgatagagg ttgattgcaa
    40681 aaatatcgtc aatcattcat ataaactttc tcatcaatta aaataagaaa acgcttgaca
    40741 gttcttttaa ccatatgata aattaaatcc tgttgctaaa acgtaactat tacggtttgc
    40801 gaaaagcgaa tcggaacatt caaatgaaag aagatgagaa gaggagtgtc ataaatgaaa
    40861 cgattgatag gtatattggc catgctggtt ttagcaggta tgttaactgc ttgcggggca
    40921 agtgggaaag ccgaggacag taaagaaaaa ttgagcgtga tgacgacctt ttatcccatg
    40981 tatgatttta caaaagcaat cgtcggggat gaaggagaag tagagttatt gattccagca
    41041 gggactgatt cccacgatta tgaaccttct gctaaagaca tggcaaagat ccaagataca
    41101 gatatctttg tttataatga tgaaaacatg gaaacatggg tccccgcaat ccaaaaaaca
    41161 ttacaagaag gcaatgtcca tacgataaaa gcgacagaag gtatgctttt acttccggga
    41221 agtgaagaag gacatgatca tgaccatgaa cacggggaag aaggccatac acatgagcta
    41281 gacccacatg tatggctagc tccaagttta gcaatcaagc aggtagcaaa catacgtgat
    41341 cagctgattg aagcttaccc agaaaaacag gaagtatgga caaaaaatgc agcagcctat
    41401 accgagaaac ttcaagcact ccatcagctt tatcaagaaa cctttaagca agcaaaacaa
    41461 agatcttttg tcacgcaaca tgctgcattc aactatctag cgttagaata tggattgaat
    41521 caagtatcga ttgctggtct ttcttctagt gaagaaccgt ctgcggcgag aattgctgaa
    41581 ctgaaacact ttgttaaaga acatggaatc aactatattt attttgaaga aaacgccaaa
    41641 gatagtattg ccagaacgtt agcaaacgaa gcaggtgtat ctttagaagt attgaatcct
    41701 ttggaaggat tgacgaatga acaaatagaa aatggagaaa actatctttc tatcatggaa
    41761 gcaaacctcg aagcgttgaa aaaaacgaca gagacagaaa acccgctaga agatactttg
    41821 acccccaaaa aagaaaagag cgtctacaac ggttattttg aagacgagga tgtacaagat
    41881 cgctctcttt cagattgggc aggaacttgg caaacagtag atacgtatgt agaagatggg
    41941 acttttgatc ctgtttttga atataaagaa aaattgaatc aggataagac agcacaagaa
    42001 tataaagaat attatacgaa gggctatcaa acagatattg cttcaatcaa gatcagtgag
    42061 aaagagatgg catttacgtt taagaatgga acaacgaaac gttcaaatta tcgttatgta
    42121 gggaaagaaa tcttgaccta cgaggcagga aaccgtggcg taaggtatct attcgaagca
    42181 gaagataagg aaagtggcgc ttttagatat attcaattca gtgatcacac gattgcaccg
    42241 gctgactccg aacattttca tttatatgca ggaaatgaaa gtcaagaagt attgctgaaa
    42301 gaaatggaga attggccaac attttatcca gatacaatgg atggacgtga aattgcacag
    42361 gaaatgcttt tgcactgata ggcaaaaaag aaccgaaggg tttggatcgg ctggtcattt
    42421 agtcggtcca aactattcgg ttctttttat gaaatcaatt tctttaatcc acggatagag
    42481 gctgggatgg tctcgccgtt tgccaacgtg acttctctgg tttttttatt aatcgaccgc
    42541 acattttgtg tattgataac ataggattta tgacaacgga taaaattccg atgcattttc
    42601 tcgatttctt tgatcttctc atagaattgt aattgggcat gagtcaaatg gacttccagt
    42661 ttatgtggcg ctcctagcga agtagaaaaa tattggatgt ctttgatcgg taaaaatttg
    42721 atctcatcat taaactctat tttgaagata tctttttgga tttgggacat ttgaaaatct
    42781 tcgaaaattt tgctcaggat atgctcgaca ttttctttta aatcataaag atcttctttg
    42841 acgatataat cagtcggttc gatgttgctt tttaagattt tcatcgacat ttccgtatgg
    42901 gaagtcacat agattatttt tcctaaagga tctaattcac ggatcaattt accgatcgct
    42961 acaccatcca tttcactgtc ttctagttcg atatctaaaa agtaaatacc aaaagaagaa
    43021 gttctgatct tggctgcatt gagaagttct tccggatcag aggtagaaat atggacataa
    43081 ctgtctaagt tttcaatcat gattctattt ttgatcaccg ttgataaaac ttctaattgt
    43141 tttggatcgt cttcacaaat ataaatggga atcatatcat tactccttta ttttatttgt
    43201 atataaatcg tttgagaaaa acgctggttt tcaattgatg tttcaaggaa aagataagga
    43261 ttggtctttg tcaattcatc caagataaac aagccaaggc cttcgttttt tggatttgtt
    43321 tttgttgaga accctttttg tttcaactgg tttaacggta atgtatttgt tgttgtattg
    43381 atcacacgaa tcatcacgga atgattcttc ttgagtatgg tcaattcgat ttttttcttc
    43441 tttgcagcac tagctccttc aatcgcattg tccaagataa taccaagcat acgtacaagg
    43501 tttaccgtat gtttcgtatc cagttcgatt ggctcaggaa aatgaagctg gacgatcaac
    43561 cctttttcct gtgccatcat gagttttaat gacagtaaac tacggatctc aggaactttc
    43621 atattatcca acatacttag attggccgga aaaaggttca tcatctggcg tgtaggtaat
    43681 atcgtttgat cataataaga ccgtaatcct tctaagtcgc cttctgaaat atagctgtga
    43741 agagaaaaaa gcagattttg gtaatcatgt ttgaattctc tcaagtcttg atagtttttt
    43801 tctagatctt tgatataatc ctgttgcatc ctgacttgtt gctctaataa ctgatcagct
    43861 aatttcttat tagagatttt ccaataataa accaagatga tcaggccaac acacagatat
    43921 agaatgaaaa aactcgtatt caaagctaag acatcaggtg tttcaccaga aaaacgggta
    43981 taaaaaataa tgatatagta tgtaaataat atcaatgtcc cgatcgtacc gatcatacgg
    44041 cggtccacag tgtgaaacca agtctctaac gattttttta aacaaaaagc taataaagca
    44101 gataaaaaag cggataagac aatgtgaaaa tactgcaatg aatctcctgg ttcaataacc
    44161 gaagatttca aaacaaaaaa atcgccgatg cttgttaagt gatctcctaa aagcgacgtc
    44221 aataacccaa aaggaagcgc gtaaaccaat tgttgacgcg aagtcgaaaa gaagaaaaaa
    44281 gtaagcatca gcattcctat agcaattaga gcagtaaaaa tccctaaaat cgaatagacc
    44341 catccaatca aaatacaaga aatcagaatg actcccgcaa acagatattt gtatcggatg
    44401 tgatggtcaa tcaataagat acaaattgtg taaatcaaat attgcaacgc tatggaccat
    44461 aaaaacataa agtacagctc ccattgattt ttaccaaatt atagcacaat tggcgtatta
    44521 tttttttaat ttttcaggga ttttgaattc agaactcata ccaaagcagc agccagatac
    44581 aaaaaagtca gataagttag ttaattgttt aatgattgtt tttttcatga aagattcctc
    44641 caaaattcat caagtagttt gtaataggaa gcaacgtagc tatttctaaa acaatcccaa
    44701 gtagcaaata ggtagaaaag taagaagctg agaaaaataa cgtaatgaga gaaaaaatta
    44761 gaaacatgct gatcgccttc tttaataatg ggggacgttt ctcgggttca atgggattga
    44821 ttgctgtacc tcttgatcca tagcgaagca gtaacaaaaa gcagctgatt aacaaaaaga
    44881 ccatcattgg gaaggagata gtcaattgca gatgtttcat caccaaggga acgccccata
    44941 aataaagtag tgtccacagc agacaaacga attcagagtc ggaatgcaat ccataagcat
    45001 atctgcggat gcagatataa gaaagaaagg tgattcctgt ttccgcccat gagcctgtca
    45061 ccagagaaaa gactagaacg accgctagtt ttccaagatt attcaataaa acttctaaga
    45121 caaattttac ttggacatac accatatctt cttccgattt gtctccgctg aataaggctt
    45181 ctaataattt gttcacaact ttatctttca tctcatacgt acctccacca caaattttat
    45241 gctaaaaaaa ataaaaaacg tcttttgttt cataaaaagt ggttttgaca tcaaaaccgt
    45301 catgatctcc taaaaaaatc ttcgttttcc aagaacctta ccaaaaaaag tgacagtttt
    45361 gatacgaaaa gtacttttta tgacaagttt gtgctttttc gagtgattta tgttaaaaaa
    45421 ttactgacct tataaaaggt gatggaggga ttcttatgga aaatgaacaa ttgtttgaag
    45481 tcggcgaaac atatgcattt aaatctgatt tatttgaatc accaataaaa ggaaaaatag
    45541 aaaaaatcta tgaaaatagt gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg
    45601 ataccgctca taatctgcac tggcagttag ttgtacgcaa atcaagtaca atagaagaat
    45661 aaaaacagat aaaatgttga gagcataaag aggttgtgag tctcccgttt agctctgagt
    45721 tacaaggaat aatgttaaaa aacagctttt catgttttat gaatattgcc ttgtgaccga
    45781 ggtgttggca ggagaatact gtttatttga aaaaagggct gcgacaagac ttttgtcaca
    45841 gcctttattt tcatgaatag gagttacttt tggctacttt tagagaagta gcttttaagg
    45901 agcagataaa gtcaaggctt ttatttctat aaatgaagta gtaaaatgaa gaacagattt
    45961 tattacagga aggatgccga taattgtgat cattgttgca ggaacgatcg gtgcagggaa
    46021 aagcacacta actgaaatgc ttgcccaaga tttagaaaca aaaccttttt atgagaacgt
    46081 ggaagataat gaagtattgc cgctatttta ttctaatcca gaaaagtata cttttttatt
    46141 acagattttc tttttgaaca aacgcttttt ggcaatcaaa gatgctttca gccatgatga
    46201 caacgtacta gaccgctcca tctacgaaga tagtatgctg tttcatttga atgcagattt
    46261 agggcgtgtt tctgaagtag aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa
    46321 ggagttagaa gaaatttcgc cacaaaagaa accagatcta ctggtttata ttcgtgtatc
    46381 ctttgaaact atgttagcga gaatcaaaaa aagaggccgg gaatatgaac agctagaaca
    46441 agatcctgaa ctctattcct actataaaga actcaatcga cgatacgaag agtggtatga
    46501 acagtttgat atatgtccca aaatagtgat cgatggggac aaatatgatt tcgtagccga
    46561 tccggcatgt ggagaacaaa tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga
    46621 agaggcagat gcccagtcga tccgctacac tacaaataac ccgaaagcaa agggaatctt
    46681 tacaactaaa taatataaaa actaagcaaa ttgctcactc gcagatattt ctctgtgagc
    46741 agcatcttgc ttagtttttt tgattcattg agatcaaata gtgaaccgat tcgtatgata
    46801 aaattgccat aaatgaaatg tctgagtgag ggccatgatc aaaaataaaa tggaaacgat
    46861 caaaatcgaa tgggcgccaa acagatgatg acagaaaaca aggacaatag ctcctgcgat
    46921 aaaggcaaga acgacactta gatagtggaa aaatcgatca aaatctgctt tttctttaga
    46981 aatacaataa tcatataagt tcgttgctac cccttttaaa tttccagttg taaataaatt
    47041 agtgtaggca cgcccgttga ttttatcaaa agctacccac tggatcgatg cagtaaaagc
    47101 aatcaaaatc gttactaatg cgggatgctc atggacgggt gtcaaactaa caaagaaaaa
    47161 aatcactgct tcataatata gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa
    47221 ataatcaatt agatactttg ttagaagaat ccctaagaaa aagaaaaggg tagacaatac
    47281 ttttttacca acacttagcc attctccgtc aaatgcttgg atgatcgata gaatgatatt
    47341 gccagtttga gcagaggcaa aagcaccata ttggatataa gtataactgt ccatggctcc
    47401 tccgataaat gtcagcaaaa gtcctattgt acgcccctcg tgaaaggaag tctgcatttt
    47461 tttcatcacc ttttatgaat atttttgtct tccttatctt agcataaacg attgaaaaaa
    47521 aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt
    47581 ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaacaa
    47641 attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa attctccgat
    47701 tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa gtctttcggc
    47761 tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc ggacttattg
    47821 aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa atgatggaga
    47881 aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat gatatgaaga
    47941 aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag
    48001 ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg atcactttta
    48061 caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag agaaaaaccg
    48121 ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa atttgtcgaa
    48181 aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat actgcacaaa
    48241 aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata tttgatcaat
    48301 atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac ctttcagtag
    48361 aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat gcacgacagc
    48421 cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa agaagccagc
    48481 aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag aatgaggaga
    48541 tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt caagatacta
    48601 atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc aagttgattg
    48661 ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca gcatacatca
    48721 agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat tatcgttctc
    48781 gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat gatcggatac
    48841 caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat ttgttttctg
    48901 atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt caaaaaaatg
    48961 ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag cagcttgtcc
    49021 aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca tatgaaggta
    49081 tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg caaacagcac
    49141 tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg atggcagact
    49201 gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa aatatagccg
    49261 ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca gaaatccgag
    49321 agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg cttgttcaaa
    49381 gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa gaatggatac
    49441 gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg caagaaaccc
    49501 aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga aaacttgatg
    49561 cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat gtctttttga
    49621 ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa gtaatcgact
    49681 ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga ctattttatg
    49741 ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa
    49801 gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt
    49861 gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg aatgtacgaa
    49921 aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta tttctggaac
    49981 acagtttatt cggaaaaggg aggtgtgaca agaattttgt cacacctccc ttatttattt
    50041 tgaaaatgag aatactatta tcaaaataga gaatatatag aaaaattctc attattcaaa
    50101 cgtaaataat cagttatttt gatttttttt atattttatt agctaataat tggtatttta
    50161 aaagaaatta ctgtagaatt ggattataat taaaaaggag tgagaaaatg ataaaagtat
    50221 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
    50281 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
    50341 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
    50401 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
    50461 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
    50521 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
    50581 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
    50641 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
    50701 catcaattgc cttatcaatt tgttgactta gaagaacgcg atttaagtac agaagaagca
    50761 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
    50821 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
    50881 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
    50941 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
    51001 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
    51061 gttatgtgtt ctttttttga ctttatttt
//
LOCUS       DACDHL010000012        50119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDHL010000012 DACDHL010000000
VERSION     DACDHL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.012"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            21..473
                     /locus_tag="IV228_000730"
     CDS             21..473
                     /locus_tag="IV228_000730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716669.1"
                     /translation="MEKEYFSNTLDIPVKTSSKRNKPDIRLDIILQDKYYIGSLIFEV
                     KYKKLDNILDNDKGRQRQQLMAYKQNTMSSILAFPEILTRSLQAVSAVFALYPSNEGR
                     KKTAPKYYEKEGIFFHLLNPSSDEKELSVKIQTSIEERISLFNQQSRN"
     gene            complement(663..1142)
                     /gene="rlmH"
                     /locus_tag="IV228_000731"
     CDS             complement(663..1142)
                     /gene="rlmH"
                     /locus_tag="IV228_000731"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8716670.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1570..2859
                     /locus_tag="IV228_000732"
     CDS             1570..2859
                     /locus_tag="IV228_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8716671.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2898..3503)
                     /locus_tag="IV228_000733"
     CDS             complement(2898..3503)
                     /locus_tag="IV228_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8716672.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3708..4172)
                     /locus_tag="IV228_000734"
     CDS             complement(3708..4172)
                     /locus_tag="IV228_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8716673.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4194..4514)
                     /locus_tag="IV228_000735"
     CDS             complement(4194..4514)
                     /locus_tag="IV228_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8716674.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4658..5737)
                     /gene="pepA"
                     /locus_tag="IV228_000736"
     CDS             complement(4658..5737)
                     /gene="pepA"
                     /locus_tag="IV228_000736"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8716675.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5937..6287
                     /locus_tag="IV228_000737"
     CDS             5937..6287
                     /locus_tag="IV228_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8716676.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6510..7457
                     /locus_tag="IV228_000738"
     CDS             6510..7457
                     /locus_tag="IV228_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8716677.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7514..8932)
                     /gene="pepV"
                     /locus_tag="IV228_000739"
     CDS             complement(7514..8932)
                     /gene="pepV"
                     /locus_tag="IV228_000739"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8716678.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            9081..9926
                     /locus_tag="IV228_000740"
     CDS             9081..9926
                     /locus_tag="IV228_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8716679.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9930..10370
                     /locus_tag="IV228_000741"
     CDS             9930..10370
                     /locus_tag="IV228_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8716680.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10420..10614
                     /locus_tag="IV228_000742"
     CDS             10420..10614
                     /locus_tag="IV228_000742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716681.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10750..11363
                     /locus_tag="IV228_000743"
                     /pseudo
     CDS             10750..11363
                     /locus_tag="IV228_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(11771..12460)
                     /locus_tag="IV228_000744"
     CDS             complement(11771..12460)
                     /locus_tag="IV228_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716682.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12435..14564)
                     /locus_tag="IV228_000745"
     CDS             complement(12435..14564)
                     /locus_tag="IV228_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8716683.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14612..15949)
                     /locus_tag="IV228_000746"
     CDS             complement(14612..15949)
                     /locus_tag="IV228_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8716684.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(16201..16560)
                     /gene="rplT"
                     /locus_tag="IV228_000747"
     CDS             complement(16201..16560)
                     /gene="rplT"
                     /locus_tag="IV228_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8716685.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16613..16813)
                     /gene="rpmI"
                     /locus_tag="IV228_000748"
     CDS             complement(16613..16813)
                     /gene="rpmI"
                     /locus_tag="IV228_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8716686.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16885..17409)
                     /gene="infC"
                     /locus_tag="IV228_000749"
     CDS             complement(16885..17409)
                     /gene="infC"
                     /locus_tag="IV228_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8716687.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            17729..18361
                     /locus_tag="IV228_000750"
     CDS             17729..18361
                     /locus_tag="IV228_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8716688.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18363..19268)
                     /locus_tag="IV228_000751"
     CDS             complement(18363..19268)
                     /locus_tag="IV228_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8716689.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19353..20006)
                     /locus_tag="IV228_000752"
     CDS             complement(19353..20006)
                     /locus_tag="IV228_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8716690.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            20328..21272
                     /gene="mvk"
                     /locus_tag="IV228_000753"
     CDS             20328..21272
                     /gene="mvk"
                     /locus_tag="IV228_000753"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8716691.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            21265..22242
                     /gene="mvaD"
                     /locus_tag="IV228_000754"
     CDS             21265..22242
                     /gene="mvaD"
                     /locus_tag="IV228_000754"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8716692.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            22255..23340
                     /locus_tag="IV228_000755"
     CDS             22255..23340
                     /locus_tag="IV228_000755"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8716693.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23337..24401
                     /locus_tag="IV228_000756"
     CDS             23337..24401
                     /locus_tag="IV228_000756"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8716694.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24650..25453)
                     /locus_tag="IV228_000757"
     CDS             complement(24650..25453)
                     /locus_tag="IV228_000757"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8716695.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25473..26315)
                     /gene="kduI"
                     /locus_tag="IV228_000758"
     CDS             complement(25473..26315)
                     /gene="kduI"
                     /locus_tag="IV228_000758"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8716696.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26331..27338)
                     /locus_tag="IV228_000759"
     CDS             complement(26331..27338)
                     /locus_tag="IV228_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8716697.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27345..27989)
                     /locus_tag="IV228_000760"
     CDS             complement(27345..27989)
                     /locus_tag="IV228_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8716698.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(28089..28847)
                     /locus_tag="IV228_000761"
     CDS             complement(28089..28847)
                     /locus_tag="IV228_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8716699.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28974..30125)
                     /gene="fucO"
                     /locus_tag="IV228_000762"
     CDS             complement(28974..30125)
                     /gene="fucO"
                     /locus_tag="IV228_000762"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8716700.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(30207..30518)
                     /gene="rhaM"
                     /locus_tag="IV228_000763"
     CDS             complement(30207..30518)
                     /gene="rhaM"
                     /locus_tag="IV228_000763"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8716701.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30520..31365)
                     /gene="rhaD"
                     /locus_tag="IV228_000764"
     CDS             complement(30520..31365)
                     /gene="rhaD"
                     /locus_tag="IV228_000764"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8716702.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31379..32635)
                     /gene="rhaA"
                     /locus_tag="IV228_000765"
     CDS             complement(31379..32635)
                     /gene="rhaA"
                     /locus_tag="IV228_000765"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8716703.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32632..34092)
                     /gene="rhaB"
                     /locus_tag="IV228_000766"
     CDS             complement(32632..34092)
                     /gene="rhaB"
                     /locus_tag="IV228_000766"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8716704.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            34245..35114
                     /locus_tag="IV228_000767"
     CDS             34245..35114
                     /locus_tag="IV228_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8716705.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(35240..36139)
                     /locus_tag="IV228_000768"
     CDS             complement(35240..36139)
                     /locus_tag="IV228_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8716706.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(36304..37128)
                     /locus_tag="IV228_000769"
     CDS             complement(36304..37128)
                     /locus_tag="IV228_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8716707.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(37139..38434)
                     /locus_tag="IV228_000770"
     CDS             complement(37139..38434)
                     /locus_tag="IV228_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8716708.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38450..38929)
                     /locus_tag="IV228_000771"
     CDS             complement(38450..38929)
                     /locus_tag="IV228_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8716709.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(38935..39963)
                     /locus_tag="IV228_000772"
     CDS             complement(38935..39963)
                     /locus_tag="IV228_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8716710.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(39980..41086)
                     /locus_tag="IV228_000773"
     CDS             complement(39980..41086)
                     /locus_tag="IV228_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8716711.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(41315..43912)
                     /gene="adhE"
                     /locus_tag="IV228_000774"
                     /gene_synonym="adhC"
     CDS             complement(41315..43912)
                     /gene="adhE"
                     /locus_tag="IV228_000774"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8716712.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(44217..44597)
                     /gene="yajC"
                     /locus_tag="IV228_000775"
     CDS             complement(44217..44597)
                     /gene="yajC"
                     /locus_tag="IV228_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8716713.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(44694..45839)
                     /gene="tgt"
                     /locus_tag="IV228_000776"
     CDS             complement(44694..45839)
                     /gene="tgt"
                     /locus_tag="IV228_000776"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8716714.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            46057..46755
                     /locus_tag="IV228_000777"
     CDS             46057..46755
                     /locus_tag="IV228_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8716715.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(46923..49562)
                     /locus_tag="IV228_000778"
     CDS             complement(46923..49562)
                     /locus_tag="IV228_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8716716.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT        14411 a        10720 c         9117 g        15871 t
ORIGIN      
        1 atcttgtata taataattcc atggaaaaag aatatttctc caatacacta gatatcccag
       61 taaaaacctc ttcaaaacga aataaacctg atattagatt agacattatc cttcaagata
      121 aatactatat cggaagctta atatttgaag taaaatataa aaaactagac aatatacttg
      181 ataatgataa aggtaggcaa cgtcaacaat taatggctta taaacaaaat actatgtcaa
      241 gtattctagc atttcctgaa attcttacac gttctttgca agctgtctct gctgtctttg
      301 ctttatatcc ttccaatgaa ggaagaaaaa aaactgcacc taaatattat gaaaaagaag
      361 gtattttttt tcacttatta aatccttcat cagatgaaaa agagctttca gtaaaaattc
      421 aaacaagtat tgaagaaaga atttcattat ttaatcaaca aagtagaaat taaaaagatg
      481 aagagcgtct ctttatacaa gagagcgctc ttcatcttca attttttgta acaaatttga
      541 atacataata aagttattat tatttttttg aaattctcaa cgattttcaa gttaactttt
      601 ttatatataa atttcaatga aaagctaact tagtgttcag gagcattcaa tcttaaatat
      661 cattatttat gatacggagc ccccgcattg atccggaagg cacggtagat ttgctcgaca
      721 aggatcagac gcatcaattg atgtggatac gtcatttttc caaaagagat ctgagcattg
      781 cttcttttca tgacggcttc gctgagtcct aaagagccgc cgataacaaa aacaaggtgg
      841 ctttttcctt ggatacctaa ttggtcgatt tgtttagcaa aagattcact tgtaggattt
      901 ttcccttcaa ttgctaaggc atagacgtat tcttgttctt tgattttcgc taagatccgt
      961 tccccttctt tttcttttac ttgtctcatt tgcgcttcgc ttaaattctc cggggctttt
     1021 tcgtcaggaa cttcgatcaa ttcgattttt gcataggcat tcagccgttt gacatactca
     1081 ttgatacctt ggatcaggta tttttctttt aattttccta ctgagataat tttgatattc
     1141 atgttttctc tcctttggtc ttcatgctca ctttgtttat tataatcaat tttttcgtct
     1201 atatgtttat tttggctatt tttgttaaaa agatttcccc aacagttatg cacaacatcc
     1261 acaggtttat ccacagaaaa cagtgttatt ttaccttatt tcatcacatt cattaggcaa
     1321 acatacgttt tataagaatt tatccacttt atacacaggc tgtggatatg ttttatccct
     1381 tatttttcca atcctatccg cgtagtttta cacagtatct gtggaaaaag aaaatcaaat
     1441 attttcatct gaaaacaatg atttttccta aagtttttca taaaaagttc acagtctcaa
     1501 agtagactaa agtcaacaaa taagactata ctaacagtag ataaatgaat gtaattggag
     1561 gaggaacaca tggatagaaa aaatgtgaca ccaaagatga aaaaaagtaa aaataatagc
     1621 ctctggcgta agttaggtct ggggcttgtt ggagggatcg ttggaggact actgacagct
     1681 ggcatttttt atgcagcgat gggcactggc aacaatagtt ctactgctac aagcggcaat
     1741 cagaattctg ccggtgaaac agttgtggaa aatgtcaaag tcaatgtaga ttctgatatc
     1801 accaaagcgg tagataaagt tcaagacgct gtcgtttctg ttatcaactt acaaagtcaa
     1861 aaccaatcaa gcggttttgg cggactattc gggcagcaag aagaaagtag cagctcagat
     1921 gatagtaatt tggaagctta tagtgaagga agcggcgtga tctacaaaaa agacgggaat
     1981 actgcttatg tagtcacgaa taatcacgta gtagatggcc agcaaggtct tgaagtcttg
     2041 atgaaagacg gtacgaaggt aaaagctgaa ttagttggaa cagatgctta ttcagattta
     2101 gcggtgttga agatcaattc agacaaagtc gaaacagttg cctcttttgg tgactcaagt
     2161 gctctgaagg ttggtgagcc tgcgattgcg attggttccc cactaggatc tgaatatgca
     2221 aattctgtca cttcaggaat catttcttcc ttgaaccgac aagtgacaag tacgaatgaa
     2281 tcgaacgaaa cagtcaatat caatgcgatc caaacagatg cagcaatcaa ccctggaaat
     2341 tctggtggtc ctttagttaa tatcgaagga caagttatcg gtatcaactc aagtaagatc
     2401 gcaagtactt ctgaatctac ttctaatgta agcgtagaag gaatgggatt tgctattcca
     2461 agtaacgatg tagtgaatat catcaatcaa ctggaaaaag acggaaaagt gacacgtcca
     2521 gctctaggta tcacaatggt cgacttatct gccgtttcta ctcagcaaca agaacaaatc
     2581 ttgaagattc cttcatctgt aactaacggt gtcatcgtga caaatgtcca aacagctaca
     2641 ccggctgaaa aagctggatt gaaacaatac gacgtcatta ctaagatcga tgacaaagaa
     2701 gtttcttctg gtgtagaact tcaatctgct ttgtataaga aaaaagtggg agattctgtc
     2761 aaaatcactt actatgaagg ctcgaaagaa aaaacaacta ctgttgaatt gacgatcgat
     2821 caatcggcat taaaacaaaa tcaaaatagt gaaaattaat aaaaaagagg ttgtgacaaa
     2881 gacttgtcac aacctcttta ttttggaaat gcttctccga ctacggcatc ctcaggcaat
     2941 actaagattc ctttttttgc tggagcattt ggcaaatgca attcacgagc ggaacagatc
     3001 attccatagc tatctacccc tcgcaaagtt cctggccaga tcatcattcc atctggcatc
     3061 atagctcctg gttttgcaac gacaactttt tgtccagctt cgatattggg tgcaccgcaa
     3121 acgatttgca atactgtacc attgtctact tctgtctcag taatcgataa atggtcagaa
     3181 tcaggatgtg ggacgcatgt ttttacgtac ccgacaacga tcttcggttc atgatcagct
     3241 tcaagctgtt cttcgaatcc tgcttctttg atcaatcgat tcaatttcgc taattcctca
     3301 tctgttaagc ttacttggcc gttcccttcg attgttagat gatcggagat atggaaaaag
     3361 ttccaagcaa caacttgacc ttgttcatct ttgatccgag caacattcca tttgcgttca
     3421 acagtgtttt ctgttccttt atcgtcggct ataataacca tcaaggtgtc tccgacgtgt
     3481 gctttgttat aagcaaaaat catgttcttt cctccattat cctttgctga gatttatctt
     3541 aacaaaaaat tgcattcact gcatgaaatt tttttcatgc tttatttccg aagaagcctc
     3601 ttctggaaca ccatttattc gtaacaaggg actgtgacaa gacttttgtc acagtccctt
     3661 tactgtttta gttaattgat taaaaaggtc gtacaagttc cacttgctta attacttggc
     3721 tgttcttctt cgtcagaaac gatcaagaca tcacaaggtg aatgacgtat cacgtagctg
     3781 gctacacttc ctgtcataaa gcgttcgact gcatttagac ctgactgccc aaccatgatt
     3841 agatcgatct catatttttt tggcaatttg actgccatgg attcacgaac agaaccatat
     3901 gcaataattc cttctatatt tgtgtaatca acagatttcc catattcttt acattcttcg
     3961 atcatttcct tggctgtttc agtttgctga tcgactagat tttgatcaat aggtgcatag
     4021 cccataaagt tataaaattg ctgatcgatt acgtttgcta catatacttt cccattatta
     4081 cggcgagcca cttctaccgc ttttttgaaa gcagcaagtg cttgttcact accgtcgata
     4141 cctactaaaa tattcttata ttcttgattc atcttgaccc ctcctaacag atgtcagcct
     4201 tttgttacac tttctaggaa cgattcgatt tcctgttttg ttttacgatc tttattcact
     4261 agacgaccta attcttttcc gtcttggatc acgacaaaac ttgggatacc aaaaatgttc
     4321 cattcagcgg ctacatctat gtacttgtca cggtctactt caataaattg ccaatcagaa
     4381 aagtcatttt caatttcagg catttgcggt ttgataaaac ggcagtctcc gcaccagccg
     4441 gcagtaaaga aaaatacact ctttccagtt tctacgtagc ctgctaattc ttctaaacta
     4501 tttgggataa tcattctttg tcctctactt tctgtttatg tatttttcaa ttaaattata
     4561 cacctttctt tcgaactaac aaacattgat cctcttttta taaaaaaaat ctggcaaaaa
     4621 gcaatctttt gccagatttt atagtttcac tatcgcatta ttttccgtat acgatcgtat
     4681 tcactgttga tttatccaaa cctttcagtg tttgaatcaa catttctcgt gcagcttcaa
     4741 aatcgcgaat actgaacatc gtttgatgcg tatggatata acgtcccact acgccaatca
     4801 ctgtacttgg gattccttcg ttttgtgtat gagcagcacc tgcatctgta ccgccttttg
     4861 aaacaaagta ttgataaggg atattgtttg tttcagcgat atccagcaag tactcgcgca
     4921 aacgaggaag catgatcaaa cctggatctt ggatacgcat caatgtacct tctcctaaat
     4981 gtccgaatgt gccatttttc gtcactgtat catctgctgc agagcagtct actgcaaaga
     5041 acagatctgg cttgaattta ttgacagaag ctttagaacc acgcaagcct acttcttcct
     5101 ggacattcgc gcctgcaatc aatgtatgac ctaatgtttc attttgcaac gcttctaaag
     5161 cttctaatac cattgtacag ccatagcggt tgtcccatga tttactgata atattctttc
     5221 cgttggctgt tttgatcgtt tcagcgtaag gaacgattgt gtctcctgga aggacaccgt
     5281 aactcattgc ctcttctctt gattcaaacc cagcatcgaa caaaatatcg gttacttcta
     5341 ctgatttttg tccgcttgtt cctcgcaata aatgtggggg aacggaagaa gaaatacatg
     5401 gataattctt accagtagag gttttaagtg taaaacgctg agcagaaaca acgtaaggat
     5461 tccagccgcc tagaggtacg actttaaaaa gcccgttttc ttggatttgt gtcagcatga
     5521 atccgacttc atccatatgt gctgcgacca ttacacgggg tgcatccgct tcttcatgat
     5581 ggcgcagacc aaaaatgccg cccaaaccat ctaactgtaa ctcatctacc agcggttcca
     5641 tatgactttc catataagcg cgaacatctt gttcaaaacc gcttgttcct tgtaattctg
     5701 ttaactcttt gattcgttga aatgtttttt cttccatatg ctattcgtct aaatttcaga
     5761 ctaaactcct ctcagactta tattgatctg cggataaaat atgatttttt ctgcagaccg
     5821 atcgtattta ttatagctta ttttttctgg tagcggttat ttatttcaaa gaaaatatga
     5881 gagtgtggta aaataagcac aaggagtaac aagtgagaag aaggaggtat gtccacatgt
     5941 atgaagaaag cgaactaagc tattttaaag gcgggttagc agttggaatc ggtctaggat
     6001 tattgagcgg ggtagcttca acgatttggt atcatcgcaa aaaagtattg tcagcagacc
     6061 aagtacttga aaatatcaag tcatcttttc taaaagaagg tgcaatcgaa ggttcttgga
     6121 tctcttttga aaaggaacct attaggaaat tcgccgttcg gtcgagtgct tatcggggag
     6181 gtctttctcg aatagaagat ggcgaaactg tttattatga atttttagca gatgctcata
     6241 caggaaccgt attagaaatt tcaagaaaaa aaggtgcaac agattaatac gtcaaaaaag
     6301 tgagagaacg aatacgcacc cactattcaa aagagaggta tcatgtcctt cttttgtagt
     6361 gggtggtttt ttagatagag ctttccaaac atatattcca gtattttctt tttaaataaa
     6421 tgatatttag cgggaaaaac tagcatttcc tttgattatg aggtattttt aatttagctt
     6481 ttgttttcta tagttaagga gagtgaaaaa tggaacaaca agaaaaaatt gctgttctga
     6541 ttccttgtta caatgaggaa gcaacaattt caacagtcat tgcagacttt aaacgagaat
     6601 taccagaagc agatatttat gtctatgata ataattcgac cgatcgaaca tacgaattgg
     6661 ccgttcaagg tggagcaatc gtaaaaaaag aacctcgtca aggaaaagga aatgtgatcc
     6721 gccaaatgtt ctttgatatc gatgccgact attatttaat ggtagatggc gatgatactt
     6781 atcctgcaga agctgtacat ggtttgctcg agaaattacg ttctggtgaa gcagatatgg
     6841 tcattggtga tcgcctatca aatggaacct actttgatga gaataagaga cctttccacg
     6901 attttggaaa taaccttgtt agaaatacaa ttactagaat gtataaaaca aaaattcttg
     6961 atgtcatgac cggctatcga ggattcaatc gtatctttgt caaaagtttt cctattatga
     7021 gttctggttt tcaaatcgaa acggaattga cgatccatgc tttagataaa aaattcaagt
     7081 tcgttgaaat cccaatcgat tatcgtgatc gtcctgaagg cagcgaatca aaattgaata
     7141 cattctctga cggattcaaa gtgatcatga tgattattaa aatgtttaaa gattataagc
     7201 ctttgctttt ctttgggatt tggaccatct tcttcttttt atttggattg gtcacgggga
     7261 ttcctgtgat tcgcgaatat atgctgacaa gctttattac acgcatccct tctgcgattt
     7321 taagtactgg attgatgact ttagcacttc tttcattagt aacagggttg attttggata
     7381 cagtcgtcac gaacgctaag aaagaatatg aattgaatct atatcatgtt tatcatgaat
     7441 accacagtag aaagtaaaaa aaggatcaag actgcaaaaa taatttgcaa ttcttgatcc
     7501 tctttttaat taattatttc gccaaacgat agatggcatc tgcatagatt gctgttgctc
     7561 tgaacaagtc atcaagactc ataaattcgt ttgcttgatg cattgtatca gtatatcctg
     7621 ggaacatcgc tccataagca acgccacgtt tcaacaaacg tccgtaagtt ccgccaccga
     7681 tgatttgttc gtagcctttt tctccagtgt ggtcttcata gacttgcaat aatgtagcca
     7741 ccaatggatc atccattggt acgtaatgtg gttccattac gcgagcacct cgagtaactg
     7801 ttgcgccttc ttgccccact gtttgttcga tgtcactttg caatccgtct actgttacac
     7861 cttttggaaa acggaaattc atattgatga agttcgcttc ctctgtacca ttttcttcaa
     7921 aagcaaatag tccagcattc attgtcaatt tgcccatttt ttcatcttca taagcgactc
     7981 ctagtttttc tccataaaaa tcttcatgga cataagcagc tgctacatgg ataaattgtt
     8041 tagcagatcc taggaattca tagttatcta agaacaatgc caagaaagaa cctgcattga
     8101 taccagattg tgggcttgca ccatgtgcgc ctttaccaac taattcgatt tttacatacg
     8161 tattgtccgc ttcaatcgtt ccgcttactg gatttgcttc gatgaattgg taaaatgctt
     8221 cttccatcgc gatagctgca tctgcacttg gtacttgaag tgctgcagtt gctgttcctg
     8281 gcaccatatt ttcgcgaagt ccggaaacaa aagatttcaa gacataatct cctgcattgt
     8341 cgcctttgaa gcgtaaagct aaagaaacat ttcctttttc accattgatg attgggaatt
     8401 cagcatctgg tgagaaacca aaatctggtt cttcttcatg ttcgaaataa taagccatat
     8461 cgccccagcc gctttcttca tcgctgccga ctacgaagcg aacttttttt gatagtttca
     8521 agtcaagttc tttaatgatc ttcatcgcat agtaagctgc catacttgga cctttgtcat
     8581 cgcttgatcc gcgtgcaaag atctttccat ccttgattac tggttcataa ggatctgtgt
     8641 cccagccatc tcctgcagga acgacatcca tgtgtccaaa tatccctagc gtttcgtctc
     8701 cttcgccaag atcaatatgc cctgcatagt tatcgacatt ttttacttta aatccatcac
     8761 gttcgccata agctaacata tgtttcaatg cgtcgcgagg tccaggtcca aatggtgcat
     8821 ctggtgttac ttttgagtca tcgcgctcgc tgttgacacg taaaagattt ttcaagtctt
     8881 caagcaagtc ttctttacgt gcttctactt ctttttgcca atcaattgtc ataaagttaa
     8941 tcctccactt atttaagtct tcgtgttcat gataccacaa attacagaaa aaaatagttc
     9001 aaaaaatctg acaattcctt ttcttatctg tttttctcta aaaaaaatat acgctataat
     9061 gagtaaaagg aggtttttag atgctgataa acgaacaact agtgaaaaga gtcatcacac
     9121 ctcgtccaaa aatttcacat aaaggaacat ttggacgtat cgttttgatt ggcggcaatg
     9181 aacaatatgg cggagcaatc attatgagtg ctttagctgc cgtacacagc ggagcgggtc
     9241 tcacgactgt agtaactgat gaaaaaaatc ggagtgctct tcacagtcat ctgccagaag
     9301 caatgattgt agtttggacg gatactgacc agtttctttc cgtaataaag caagcagatg
     9361 tactcttgat tggtcccgga ttgggtactt cttctaagag tttggaacgt ttcaaacaag
     9421 tactagaaaa ccaatcaaaa gaacaatggc tggtgattga tggctctgct ttaagtttac
     9481 ttgctgctaa tcatcttttg ctaccctatc ccgaacaaac tgtacttaca cctcatcaaa
     9541 tggagtggca acggttgagt tctctaccga tacaagaaca acgggataaa gaaaacaaaa
     9601 agaagcaaca ggaaatcggt gctgtgctcg ttttgaaaag tcatgagaca aaaatttatg
     9661 gtcaccagca cttctacacc aatcctttag gttctgctgc tatggcaacg ggcggcatgg
     9721 gtgacacttt agctgggatg atcacaggat ttttagccca gtttcctcaa aatgaccaaa
     9781 cgattgcagc agctatttat cttcatagtt tcattggaga ccagctggca aaagaacaat
     9841 atattgtttt gccgacacaa atcagcgaag cacttccgaa atggatgaat tatttttgta
     9901 aaaaatcaat ggaaaacgag gcatgagtca tggagaaatt tacaggttat gtgcgagaac
     9961 ctttttatta tgaaacagat caaatgggga ttattcatca cagcaattat atccgctggt
    10021 ttgaagaggc tcgggtagcc ttattggtac atctagggtt tccctataaa aaaatcgaag
    10081 aatctgggat catcatcccc gttctggaag taaattgcag ctataaagaa atgatccatt
    10141 acggagaaaa agtgagcatc catccaacta tccaaaagta tacggggaca cggttggatt
    10201 ttagctacga aattgtggga actgaagaca aaaaattaaa aactactggc agtagtaaac
    10261 actgtttttt actttctgat tcgcaacgat tagtgaagct gagcaaagtc aatccggagt
    10321 tagatcagtt attccaagcg tacgcttctt tggatgatgt aaattcttaa taaagaccgt
    10381 ctaagcattt ttcttgtatt gtgtcgagta gttcgtataa tgaaaagaaa aaagcaggtg
    10441 atccccatgt atgtcgttaa agtaatgcac ggttacatcg ataaaactgg ctgtcgcact
    10501 cgggaaaaaa acttagacaa tctgctcatt ttcaaagaca aaaaagaatc cgaagctttt
    10561 gctaaaagaa tcggcggtcg tgtcaaaccg atacaagagg tacgaccaga ttgattcaat
    10621 atttttcttt tcgaggctct aataggcatg cctatcaggg ctttttttgc agactgtgtt
    10681 tttatgccca cacgatttgt aagcgattta tgttataata gattatatct gcatagaaag
    10741 gaaggttaaa tggttatcaa taatttgcaa cagcaagctt atgaatctat acgtaaacaa
    10801 atcatttatt ccgagttagc acctggaagt aaaatctccg ataaagtgtt agaagaacaa
    10861 ctagctatcg ggcgtacacc gattcgagaa gcactgatcc aactgcgcaa tcaagagctt
    10921 gtaaaaacca ttccacagtc cggcacttat gtttcaaaaa tcgatatccg atctgcttca
    10981 ttagcccgtt atacgcggga aaagttggaa aaaccgatca ggcagtaatc gctcaaaata
    11041 aaaaagcttt ttttgatttg gatactgatt ttcaccgaac ttgttatgaa attgctggaa
    11101 aacgagaaat ttgggattgg ctagaaagtt atagtacgca cttgaaccgt ttcagatggt
    11161 tacgtttgac gatttcagaa ttagactggg gtcgagtatt agatgaacat cagacaatgc
    11221 ttcaatcaat gattgatcat aactttgatg aagtcggatt tctttgcacg atgcatttac
    11281 atatgattat cgaggagcaa gaatacgtga ttcataatta ccccgattat tttgaagata
    11341 ttcaggacag acccatcaaa taagaggttt tggaatcagc gttttgacct gagtattaga
    11401 gaaagaaacg gcaaaatagt tcctcatatt ttgccgtttt ttttgagcta ataccgaggg
    11461 tcagctgatt gcacaccgtt tattgagagg ttgtgaaaaa gctgccctgc atcaagtaat
    11521 aagaacagcc aagtaaaaat agcttcttat gtttttaact tggctggact tattaccgca
    11581 gatgcaagct tttgaacacc gttttccaag aggtcgtgag cctgccgttc atctccgagt
    11641 cacaaggaac aatttcaaaa aacagcttct catgtttgtt gaatattttg acttgtgacc
    11701 gaggagttga caggagaaca tcgtttattc ggaaaaaaag gactgtgaca cgacttttgt
    11761 cacagtctct ttatttgtaa tctaaatcat actccgtaca tcttttttct atttttttta
    11821 tttcttctaa gatcccttgg ctacgaattt cattatatgg ccaaagtaag actgcctcta
    11881 atacataagg cgaaaaatcg ctttgattca tctcttttga aaaactcatc gctgctatct
    11941 cgctcaatgc acgaatgttt gattgatgtg tataaaaagg caaccgccac agcacaattt
    12001 tttcgctttg cgtataaatt cccttaacag aagcttcgat gtggtgaaat agttcatgtc
    12061 caataatcaa agcttgcgct gtttctttag agatcgaagc tagtcctgaa cattttaatt
    12121 tttctaacgg ctccgtcatc aatcgaatcc cttcatcttc tgcataagtt gcaaaagtga
    12181 ttcgttcgtt tatttgctct gattcacagg aaacgattgg aacagccatt tgttcacaaa
    12241 tttctcctac atcggcaaaa gggtactttt ttttgatgct acatgccgtt tcgataccac
    12301 aatcgattga ttgttgtata agaattttct gcttggacaa aggaatcttt ttccaaaata
    12361 aatctcgccg aaactggtag aatccccaaa tttcttcggg gatctgttgt aattcgttta
    12421 gtaactcttc taacttatgt ttgtttgata tttccggcaa cgacgatcac ttcttcctcc
    12481 ggttccatag tagatacttc cagatccatc aaaagctgta gttgttccag atccgtcgct
    12541 gtaaaatcag agactcttgc tcctaagcag agataaaatt ctggtcgggc aatcaattca
    12601 gtttgataaa ctgccatatc ttcccaaagt tgttcgacag ctgtcatata attggctgca
    12661 gttgttttca tacatgaagc atgtccacgt tggaccttga taaatccttt ttgatcgatg
    12721 atccgaagac ttccagcatt ttttgaacga ttctgttcgc cgcatacata aaagaaaggt
    12781 gtattttcca ataagcaaac ttcattttta tttaatcgca tatcctctgc agcaagctct
    12841 agtgcttctt ctgttgtgat ctccttggtt agatcggttg ctttcacttc cgtagaacca
    12901 gtagcgatag ccgttacttt tgaagtctgt ggatcgattt ctacgtggac ttcgatcgat
    12961 tctggtgtag cgcctgattc aatcgcttta ttcatcgctt catttttcaa cgatcgtata
    13021 tcttctttac ttggcgaagg gatgatacgt tctacaacat ctcggaccat tgcaagtgct
    13081 acaccaatag aagaaatcac ttcggcgttt tcaggaatcg aatatttcac acccatttta
    13141 ttactaaagt aagtaatcaa tgaggcagcg ccgcccccta cacctacaag agaaatctga
    13201 tctttctcca agtgatactt gtctgccaat tctaaaatca ccggttcaat cttagcatag
    13261 gatttttcca tgatctgttc tgcgatgtct tcaaccgtcg tatgacaata atcagctaat
    13321 gcttggatgg cttttcgtgc agatgggaca tttccataag aaaaatgttc ttcttgcact
    13381 aatccaagga cattggctgc acatgtattt gtaatagtca cttcttctcc gtcttccatc
    13441 actaccttga cgtaatcagc agggtctcct tctttaggag agaaaaattc aaccttagga
    13501 cctttgattt tttctgtctc cgtaaatact gcataatcta gaccggcaat atgtgcagaa
    13561 cgtggaccga catcgatgat tcctgactga ttagcccgta ccatcgaacc gcctgccaca
    13621 cctagaaccc gaacatctaa tgaagagata tatgttgggt ggccacctac aattgaataa
    13681 tcgattgctg gacgtccatt tttaatgacc ccgatattgg ttgttgttcc gcctacttca
    13741 aaataaacac cattagacgc tctcaagtac atcaaagagc ccatgactga tgctgctgga
    13801 ccggaaagca tggttaaaac aggtcgtttt ttcatttcat tgatttccat tactccgccg
    13861 tctccgcgca taatcatcaa agaaacattg acccctgctt ctcgtacgga atcttctgta
    13921 gaggttgccg tatctagcat ttttggtaaa atagatgcat tgattgctgc tgtccgagtt
    13981 cttcttgtca atccatataa ttttgtgatg tcagaagcca tcgttgtagg gatcgatttt
    14041 accgatgctg cttcataaac aactcgttct ggttccccat tatctactcc aaatgccata
    14101 gaagaaacca agacctccgc tctttctctt tctaaagaag aaatcgtttc tgaaacacgg
    14161 tctacattta agtgtttgac aggtaagaat gcattgacaa tctctatttt tttcttattc
    14221 cctaaatcga tatcgttcaa tctagtttgt cgttttgcta aaaaaccttc taatccacct
    14281 tttgccatcc cgattacgcc gacttttgct acgtctcctt caatcagtgc attagtcgct
    14341 tgtgtggtag aatgtgcaac aaacacaaca tcttctggac tgatattatt ttcccgcaaa
    14401 caattttgaa aagactgaac gacacctgcg gctactccac ggacatcatc gtgggttgtt
    14461 ttcactgaag attttccgat gatttcgtgc gtcgcattat cgattgcgac tgctttcgta
    14521 tgtgtaccgc caacatcaat tcccattcgc acaaaacgtt tcattttctt ttcctccatt
    14581 catctaatcg tttttatcga cgttttgttt actaggcgtt cccgtacaat aagaacgtga
    14641 tcaggcataa gatcgctccg atgatccaac cagttgggat ggatttcttc atatattctc
    14701 gagaagttac ttttgtataa cctaatcccc aagcaaccca agattgtgta atatctaaat
    14761 gctgaggagc aatcgttgtg attgcaaata acgggaacaa aaaggctggc gacatcgttg
    14821 gattaacagc taatacgaca gctaaaatag ctgagcctga accgactaga ttcattggac
    14881 cgcggaaaaa tcctagtggt gtcaatatag caaatacgat tgccaagact aatggtgatt
    14941 gcggtatcca atctccaaaa atagctgaaa aataaggaga agcatacgca gctgaattat
    15001 tgaacatcgc taaggtcatc aagaaaccaa ccattggtgc aacatctact gcgccatctg
    15061 taaataattt cgcaaactga cgacagattt ctttataacc tccgcgcaat tttccagtaa
    15121 acagcaatgc ccagatacct gctagaataa aacctaagat gattggaaga tctaaaagaa
    15181 cgacccctaa aactggtaat aatacggcta accatgagat cattggtgcg tcgccacctt
    15241 cagctggtac gtttgctgcc attgcatagc gtttcttttt gttcattgat atattagcga
    15301 ccgttagtac cacaactaag cttacaatca tcccaatcat accaatttgg aaatagtcat
    15361 tataagtata gctttcagcc gctggattga aaccagcata aatcgtttga tattgtttga
    15421 aattgacgat attagcaaag attccagcca taatcgagcc cataaatgag aacagtgcaa
    15481 ccggtactga aatacctaat gacatcaaga ttggcaacac aataactgca atagaaatca
    15541 ctggtccgat tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca
    15601 tcgtgattct tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga
    15661 ttccgctatc tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg
    15721 atttggcgta ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt
    15781 gcccttccat cgccgggttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata
    15841 cagtagccat gatggcaaag ccgaccatca agttgtagcc ttttacacag taccagacca
    15901 tgaaaaagaa tgagataact aacaaaatac caataataat atcactcatg ttattcctcc
    15961 gtccattttg tttttctgat aagcaaacta atatatcagt caaactcatt ctaacttatg
    16021 tagttagcgc tttcattaaa gggtgaccat aaatgttcag ttttcaatta aaaaacctta
    16081 attatgctat tttagactta tacgttattg tcctgtttca tttgatggag gattgtaaat
    16141 aaaaaaagag gaaagaacat aacttttcgt tatcttcttt cctctttata attgtttagc
    16201 ttatttagcc aaagcttctt ttgcttggtc agccaatgca gtaaatgctg ctgcatcgtt
    16261 aacagccaag tcagctaaca ttttacggtt gatgtcgatt tcagcaactt tcaaaccgtg
    16321 cattaatttt gagtagctca agccattcat acgagctgct gcgttgatac gagcaatcca
    16381 caatttacgg aagtcgcgtt tcttttgacg acgatcgcga tatgcgtagt tgtatgaatt
    16441 cattacttgt tcttttgctg ttttaaataa agtgtgtttt gaaccgtagt aaccttttgc
    16501 taattttagc actcttttac gacgtttacg agttactgtt ccacctttaa cacgtgccat
    16561 gtataattcc tcctaataaa aataaatctt cagattctgt tatttgtact tcttatttca
    16621 tttttgctag ttgttggcga atacgtttga agtcgccact agaaaccatg cttgctttac
    16681 gcaattgacg gcgttgtttt tttgttttgc cgtggaaacg gtgacttgta aacgcgcgga
    16741 agcgtttcaa tccgccttta ccagtacgtt tgaaacgttt tgctgatccg cggtgtgttt
    16801 tttgttttgg catgactata tttcctcctc aaaatctttc gttatccgac tatcggctgt
    16861 ccaacagcca gttagtcatt caaattactt actgtcgttt ttcggtgcca gcgtcaagaa
    16921 catgctgcgt ccgtccattt tcgctttttg ttccactgtt gcaatatccg cagtttcttc
    16981 agctaagcga ttcaagactt tttgaccaat ttctttatgg gtaatggcac ggcctttgaa
    17041 acggatagaa gctttcactt tatcgccctt ttccaagaac ttacgtgcat tacgaagttt
    17101 tgtattgaag tcattcaagt cgatcgtagg acttaagcga acttctttta cattgatcac
    17161 tttttgtttt ttacgcgctt cgcggtcttt cttttgttgt tcgaaacggt atttaccgta
    17221 gtccatgatt cgcgcaactg gtggtttcgc accaggggca actaacacaa gatccaagtt
    17281 tgcttgttct gcaatattca atgcttctgc ttttgtctta actcctaatt gctcaccatc
    17341 tgaaccgatc aatcgtaact ctcgtgcacg aatgccgtcg ttaaccatca tatcctttgc
    17401 tatggtcatt cacctccaaa tattatgaga gaaagaaaag cttaccgaat aaacggcaat
    17461 aaaaaaacga gttctcttac agaacccgcg cgtacttcac ctaatagaac cagtgaaaac
    17521 tatctagccc agtgacatta tcaactaagg cgagaagcgg gtgcttctgc ttttatttct
    17581 caaccatcat agtatagcaa aaactaatct ttttgtcaat gtttttcata cagtttgtct
    17641 tccttttctg aaaatgatta gattagcaga ctttctagca gtgattcccg cttctttgct
    17701 atactaaaga aaaacgaggt gaacccacat gaaaaaaatc gaacctattc caccttctct
    17761 tcctcttctt acaaagggag agttttattt agaagatgag gagatcttct ctggtcttct
    17821 gatcaacggg ggtgaccaaa gctatctcga tgccaaaaat atggtattaa gagagagtca
    17881 tatccaaaaa cttacgatgc aaaaaacagt tttacgtcat tttgaatgca gtaatgtcat
    17941 ttttgaaaaa tgtgattttt ccaatctgga gtggattggt ggcagtttcc accaagtcgt
    18001 gtttcatcaa tgtaaattaa caggtacgaa ttttgccgaa agttatcttc gagactgtac
    18061 tttcactgat tgtattgctt cgatggcttc ctttagtagt acaaacttga aagctgtctc
    18121 cttcgatcat tgtcaattag aagacagcga attttatgag gtcacttgga aaaacctttt
    18181 tttgtctgac aatcagttga ccggttcgaa ctggtttcga acttctctga agggattaga
    18241 ctttacgact aatttcttca cagcaatcgc tttatctcaa gattctttgg cgggactaat
    18301 cgtcaaccaa gagcaagcac ttgtgatcgc ccaagccctt ggtttgcaga tcaaagacta
    18361 gatcaacgaa tccgccgcca cagtgcaata agcatccaaa tagataagac caaactgatg
    18421 atcaaggcga ttccccatcc gaaaacggaa tgggtaaagg gtaactggag attcattccg
    18481 aagaatcctg tcacaatagt tggaacggtc aagatcagcg accagaccgt caaaaatttc
    18541 atcgtgtcat tcaagttgtt attcagcaaa ttattataag tgccagagag ctggtctaaa
    18601 atctgcgaag ctaagttcgt catttcaact gcttgtcgtg cttcgatcag cgcatcgtcc
    18661 aactcttctt tttccttttc tgacaacttg cgataaatag ccaatgcctt gatttgttct
    18721 aaaagtacta cattttgttt tgttcctgta actaagtaaa ccaaaccgat ctctaaatct
    18781 gaaagttgca gcagattctt gttcgttgtt ttttctctta atttcagatt aagccgctga
    18841 cgttcactat tcacttcctc gattaaagga aaaaaggaat cagaaatcaa aaacaatgta
    18901 cgaaacaata aactgtatac actcatgtct ggcattcgtt ccaaaagcga tcgaattagt
    18961 cgaatcacgt actcggtatt cggcgtggta aatgtaaaca aaccgtcttc ttttaaaata
    19021 aaagcgatag gactagtttc ataatgatta tcgacttttt gctggtgcgg aacatttgat
    19081 acaagcagta acgcttcatc aaaagaatcg tactctacgc gcgcccgttc atttttatct
    19141 agagaataag tcagcatctc gtcactaatg ccatattctt cttttaagtt tttaacagct
    19201 agcacatcgt tttgctgtac attgatccaa aaattacggt cttgattaaa atagtgtttt
    19261 tcccgcatac tctatcctcc tgacatttgt ttccagtata aagaaaaaag tcgctcaaat
    19321 aaaggtttac cctttatttg agcaactgta aatcaaatgg ctactttggt ttggcgaatc
    19381 tctgctaatt tttcttcgat cattttcaag acttcttctt ttgcttgagg atcttccaca
    19441 aaattatagc ggtctccatc gatttggatt tttggacttt ctttgtattc ttcataccat
    19501 tgatcgtaac gtctgttcaa ttctttgtaa taatcgtata gacttggatc aaaggacaat
    19561 tgttcatacg aacgaccacg tttttcgata cgctccaaca tcgtatcaaa agatacacga
    19621 atatgaacta ataaatccgg atgtttttta tgggcagcat agggaagttc ttccatcatg
    19681 ttttccagca gttcatcata cacacgaact tctgtttctg ttgctcttcc tagatctgca
    19741 tttagatgga aaagcagtga atcttcataa atcgaacggt ccaatacatt attgtcatct
    19801 ttcattgctt gtttgatgct tgcaaaacgt ttattcaaaa aataaatttg cagcaaaaaa
    19861 gcatactgtt cagggtttgc ataaaataag ggcaagactt cattatcatc aatcgattca
    19921 taaaatgcct gactatcaaa gtgttcagcc atcatttctg ttaaacttga ttttccggca
    19981 ccaattgtac ctgctaaaac gatcacgctc atttacctcc agctcacttc atcactaaat
    20041 attgtgttta actccaacct ttttaaatat acccccaata tctagtagtg tcaattgtat
    20101 ttttttctag tcaaagaaga aaaaaagtca aaaaattctt aagaaaaaag tgttttttct
    20161 ttagaaaacg tattcttagt tttacttctg agatggtttt agtaaaataa aggaggaatg
    20221 ttttttgaga cccttagtgg acaaaaaaat tcgtgaagca gcgcaactcg ttttgggcgc
    20281 tttatttttt gcttaaaaat cacttttacg tgagaaggag ttagattatg gcaaactatg
    20341 gccaaggaga atcaagcgga aagatcatat tgatgggcga gcacgcggtt gtttatggag
    20401 aaccagcgat tgcctttcct ttctatgcaa caaaagtcac cgcattcctt gaagagctgg
    20461 atgcaatgga cgatcaactg gtttcttcct actattcagg aaatttagcc gaagctcctc
    20521 atgcattaaa aaatatcaaa aaattattca ttcacttaaa aaaacagcat gacatccaaa
    20581 aaaacttgca actgaccatt gaaagcacga ttcctgctga acgtggaatg ggatcaagcg
    20641 ctgcagtcgc cacagcagtc actcgtgctt tttatgatta cttagcattt cctttgtctc
    20701 gtgaaatact attagaaaat gtccagcttt cggaaaaaat cgcccacggt aatcctagtg
    20761 gaatcgatgc agccgctact agcagcttgc agccgattta ttttacaaaa gggcatcctt
    20821 tcgactactt ttctttgaac atcgatgctt ttttgattgt cgctgataca ggaatcaaag
    20881 gacaaacaag agaagccgtc aaagatgtcg ctcacctctt tgaaacccag cctcatgaaa
    20941 ctggacaaat gatccaaaaa ttaggatact tgacgaagca agcaaaacaa gcaatcattg
    21001 aaaattcacc agaaacgttg gcacagacaa tggatgaatc acaatcactt ctggaaaagc
    21061 tgacaatcag caatgatttt cttaatctat tgatccaaac agcaaaagat accggggcat
    21121 tgggtgccaa attaactggc ggtggacgcg gtggctgtat gattgcatta gcacaaacaa
    21181 aaacaaaagc ccaagaaatc agtcaagcac ttgaagatgc gggtgctgct gaaacttgga
    21241 tacaaggatt aggagtacat acctatgttt aaaggcaaag cacgcgcata taccaacatt
    21301 gctctaatca aatattgggg taagaaaaat gaagaactca tccttccaat gaacaacagc
    21361 ctttcgttaa cattggatgc attttataca gagacagaag tcatcttttc tgatagctat
    21421 atggtagatg aattttatct agatggcacc ttgcaagacg aaaaagcaac aaaaaaagtc
    21481 agtcagtttc ttgacctttt tcgtaaagaa gccggcctct cactaaaagc ttcagtaatc
    21541 agtcagaatt tcgttcctac cgcagctgga ttagcctcct ctgccagcgg gctagctgct
    21601 ttagcaggcg cttgcaatac tgctcttaag cttggattag atgatctctc tctttcaaga
    21661 tttgctcgac gcgggtctgg ttcagcttgc cgaagtattt tcggtggttt cgtcgaatgg
    21721 gaaaaaggcc atgacgactt aagttcttac gctaagccag tcccttccga ttctttcgaa
    21781 gacgatttag caatggtttt cgttttgatc aacgaccaga aaaaagaagt gtccagcaga
    21841 aatgggatgc gtcggacagt cgaaacatcc agtttttatc aaggctggtt agattccgtt
    21901 gaaggggatc tatatcaatt gaaacaagca atcaaaacaa aagatttcca acttctcgga
    21961 gaaacgatgg aaagaaacgg actaaaaatg catggaacga ctttagctgc ccagcctcca
    22021 ttcacttact ggtctcctga cagcttaaaa gcgatggatg cggtgcgaca attgagaaag
    22081 caaggcattc catgctactt tacgatggat gctggcccta atgtcaaagt tttagtggaa
    22141 aacagccatt tatctgaggt acaagaaaca tttaccaaat tatttagcaa agaacaagta
    22201 attactgcac atgctggtcc aggaattgcg attattgaat aaagggagat ttttatgatt
    22261 gaagtatctg caccaggcaa actctatatt gccggagaat atgcagttgt tgaaacaggc
    22321 catccagcag ttatcgctgc agtcgatcaa ttcgtgacag tcactgtaga atctgcacga
    22381 aaagtcggaa gtatccaatc tgcacaatat agtgggatgc ctgtacgttg gacaagacgc
    22441 aacggagaat tagttttaga tattcgggaa aatccttttc attatatcct tgctgctatt
    22501 cgcttgactg aaaagtatgc acaagaaaaa aacattcttt tatcctttta tgatctgaaa
    22561 gtaacaagtg agttagacag ttcaaatggc cggaaatatg gattgggttc tagcggtgcc
    22621 gtgaccgttg caacggtcaa agccttgaat gttttttacg cgttgaattt atctcagttg
    22681 gagattttca agattgcagc actagccaat ttagcagttc aagataatgg ttcctgcggc
    22741 gacatcgctg ctagctgtta tggtggctgg atcgctttct caaccttcga ccatccttgg
    22801 ctccaagaac aagaaactca gcattctatc agtgagttac ttgccctgga ttggccaggt
    22861 ctatccattg agccattgat tgctcctgaa gatttacgtt tattgattgg ttggacgggt
    22921 agccctgcct ctacttctga tttggtcgat caagttcacc gttcgagaga agataaaatg
    22981 gtggcttatc agcttttctt aaaaaatagt acagaatgtg tcaacgaaat gatcaaaggg
    23041 tttaaagaaa ataatgtaac gttgatccaa cagatgattc gaaaaaaccg acaattactg
    23101 catgatttat ctgcaatcac tggggtcgtc atcgaaacgc ctgctttgaa caaattgtgt
    23161 aatttagctg aacagtatga aggagccgca aaatcttctg gtgcaggtgg gggcgattgc
    23221 ggaatcgtaa ttgttgacca gaaatctggc attcttcctt taatgagtgc atgggaaaaa
    23281 gcagaaatca ctccactgcc gttacatgtc tatagcgatc aaagaaagga aaaccgatga
    23341 atcgaaaaga cgaacatgtt tcactagcta aagcctttca tgataaacaa aaaaacgaat
    23401 ttgacttcgt acggatcatc cataaccctt tgccgcaaat agcagtttct gatgtcgatc
    23461 taagtacaca ggcagttggg tttacactaa gcagcccatt ttatatcaat gcgatgactg
    23521 gcggaagcga aaaaacaaaa aaaatcaacc aagatcttgc aatcgtcgca agagaagcag
    23581 atttgatgat tgccactggt tcagtcagcg ctgccttgaa agtcccttct ttagccgata
    23641 cttatacgat tatgcgacaa gaatatcctc acgggaaaat cattgccaac atcggtgcag
    23701 ggacttctgt cgaaagagcc aaagaagcta tccgattgtt tcacgcagat gccttacaga
    23761 ttcatttgaa tgcacctcaa gagttggtta tgcctgaggg agaccgtgat tttaccaatt
    23821 ggaaagtact tattcaagaa actcaaacag ccattgacgt ccccctcatc gtaaaagaag
    23881 tcggttttgg catgacaaga gaaacgctca atgatctagc tgctttagga gttcatacag
    23941 ttgatatcag cggtcgaagc ggaacaagct tcacccagat cgaaaacgcc cgtcgctcca
    24001 agcgggaact gagttattta gctgactggg ggcaatcgac agtctcttca ttgcttgaag
    24061 caaatgaagc agacacttct atggaaatcc tagcctccgg aggtatccgc aatgcctatg
    24121 acatatttaa agccctttgt ctcggcgcaa atgccgtggg tacttcagga accgtactga
    24181 cgcatctgat gaatcacggc gtagaagaaa ctattatact gatgaagcaa tggcaagaag
    24241 agcttcgtct gctttatact atggtcggcg caacgaatac cgcggcccta caccaacaat
    24301 ctctgatctt ttccggacca gtgaaggatt ggtgcgaagc aagagggatc gacttagtga
    24361 agtatggaag acgcacagaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg
    24421 ctctaaacac gtacaaacgg cggaagtaaa agcaactcct tcggaaataa gctgaaattc
    24481 acaaaaattt gaaaagcaat tttcgtgaat tccttcttat ttcttggagt taaatgcttc
    24541 tgtcccagcc tcttttttcg aataaatgat gtttaatcag ctgtccttcg gtattcgttc
    24601 aatccgctga tttcacaacc tctccactaa acagtgtact aaagctcttt taaggttgtt
    24661 ttccgatata ggcaagtatg cctccgtcca catacaagac atggccgtta acgaaatcag
    24721 atgcgctaga agccaagaag acagatggtc cagccaaatc gattggctcg ccccagcgtg
    24781 ctgctggcgt acgtccaacg ataaattgat cgaatggatg acgttcaccg ttttcttgtg
    24841 tttcgcgtaa tggagcagtt tgcggtgtag caatataacc tggtccaatt ccattacatt
    24901 ggatattgta ttgaccgtat tcagaagcga tgtttttcgt cagcattttt aatccgcctt
    24961 ttgccgcagc ataagcactg accgtttcgc gtcctaattc actcatcatg gagcagatat
    25021 tgatgatttt accgccgcct ttttcgatca tatccggaat aactgctttt gaaacgatga
    25081 atggggcatt taaatccacg tcgattactt ggcggaagtc tgccgcatcc atttcgatca
    25141 tcggtgtccg tttgatgatc cctgcattgt tgactagaat atcaactgaa cctacatctt
    25201 ctttgatctg ttgaaccatc gcttgaaccg cttcttcatc ggtcacatcg cagacataac
    25261 cttttgcatc gatgcctgct tctttataat gcgctatacc ttcatcaact gattcttgat
    25321 tcaagttatt aaatacgatt tttgctcctg ctgtagctaa agagcgtgca atctcaaatc
    25381 caataccata gacagcaccg gtgactaatg ctacttttcc atctaaacga aacatatcca
    25441 tactaaattc catcattttc tctcccttat gcttatttta attgatccat ttgcaccata
    25501 tccatgtctg tataggtgat attttctccg cacattgccc agatgaatga gtaattgctt
    25561 gtacctacac ctgaatggat cgaccagctt ggcgaaataa tggcttgttc attgtctaca
    25621 actaaatgtt ttgtttcatc tggtttgccc atcatgtgga acacacgtgt atcttcattg
    25681 gcataatcaa agtagacata agcttccata cgacgttcat gtgtatgaca aggcatcgta
    25741 ttccaagcgc ttcctggttc taaaatcgta taacccattt gtaattgaca gctttcgcat
    25801 acatttggat ggatgtattg gtagatttta cgttcattca gtgtcaggcc ttcaccagtc
    25861 tccattggtt ttacttggtc gatactgatt tttacatttg gatatttatg atgagctgga
    25921 acagaactaa tatagaattt agctgggttt tcagcatctt gagaagaaaa tacaacatgt
    25981 cgtgtttctt ttccgatata gtatccgtct tgttttttca tttcttcttt ttgcccatcg
    26041 atctcgatga atcctggtcc accgatattg atcacaccta gttcacgacg ctctaagaag
    26101 taatcaacac ctagttcttt gtctaatgtg atttctaatt ctttttcagt aggtgtcact
    26161 ccaccaaaga tcattcgatc gttgtgcgta taagtcaaac tgattttccc tggttcaaat
    26221 accttttcta ctaaaaattc ttttctcaat tgttccgttg aataatgacg gatatcttct
    26281 ggactgtgtg tataacgtgt ttccatttct tgcattttct tctactcctt ctatcgaatg
    26341 atttttccag agccgcatgc taaaaattgt gcgacttctt cttgactaaa ttggttgcag
    26401 tccccatgga tcgtatgctt caaggcagaa gctgccgtag caaaatcaat gatttcttgc
    26461 ggagacatat tggataaaat cccatggagc acgccacctg caaaagcatc tccgccacct
    26521 actcgatcca cgattggggt gattctatgg acttgggatt ccacatattc atggttcatc
    26581 cacaatgtcc ccttcaattc attttcactt gctgaatgaa cggtacgctt ggtcgaataa
    26641 aacacctgaa tattcgggaa cttttcctgc attttttgat aataataaat tagaggttgt
    26701 tcttctgctt ctggcgcttt ctcgatacct aacaaataaa gagcatccat ttttccagct
    26761 gagcagatat ctactaaagg taaaagctga tggattactt cgctggcttc ttgctgtgtc
    26821 cataattttc cacgatagtt gatatcgaag ctgatcaggc agccagcttt ttttgcagct
    26881 tctatcacct gtttcgtcat tttttgccat tttttagaca aagctggggt gattcctgaa
    26941 acatgaaata tatctattcc ttggaataac tcctctagcg accattccaa atgatccatt
    27001 tcagcaaaac ttgatccggc acgatcatag atcactgagg cggcgcgttc gccactacca
    27061 gtttccatat aataagtacc tagtcttgat ccaccagata atacctgttt cgtatctact
    27121 ccataacatt gcaagtgctt tttgactgct tttcccaatg cgttatcagg tacttttgta
    27181 gcaaaaatcg cttcatgtcc gtaattagct aaagaaattg ctacgtttgc ttcccctccg
    27241 ccataatggg cagcgaatcg ttcacttgaa tcgatacggg ttccctgctc tgtggacaat
    27301 cgcaacatga tttctccgag tgtcactact tttcccataa gcagctacct cttgatttct
    27361 ttcatttttg ctgcatactg ttgcgcgact tccgttactt tgtcaaaatc tcctgtagct
    27421 gcgggtgcta aaaggttccc acctactccg actgttacga caccagcatc gaaccactct
    27481 ttcatattgt ccagactcac accgccagtt ggcatgatat tgacttgagg catcggcgcc
    27541 ttgaaggcag aaatgatact tggtccataa gcgcttccag gaaatagttt gacgatatct
    27601 accccgcttt tcagcgctgt tttgatctcg gtaatagtca tacatcctgg taagtaagga
    27661 atttgataca gattgcacat ctctgccgtt tcttgatcaa aggaagggct aacaatatac
    27721 tctgcgccag ctaagatagc caagcgagct gtgacagcgt ctaatactgt gcctgcgcca
    27781 atcactattt ctggttggtc tttgtagaat gacaaaagtt ctttgattac ttgatctgct
    27841 tgagggactg taaaagtcaa ctcaatacca gttagaccac ctttgatgat tgcgtggcaa
    27901 gcattcaaag cttcttcttt gctttttccg cgtaccaccg caatcactcc ggcgtttttc
    27961 aatcgagata aaatatcaat ttttttcatc ttctccacct atttcctatt tagaaatttt
    28021 attttataac ttggaaatct atacccatat tatacggcag aaaagaaaaa ttgcaaacgc
    28081 ttttttcatt atttttgaat tctttgctcg atttcgcttt ttgtttgctt gagggcactg
    28141 attatttgtt cttttgtctc atctgttaaa cgatatttcg gcatacttac actaaatgca
    28201 ccaatgattt gttcgttttt cacgattgag gctcctatac aaaaaatatc acgttcgatt
    28261 tcttcatcgt caaatgcgat gtgggtttct tttaccgttt ggatttcctt tttcaaccgt
    28321 aatgcattgg tgatcgtata ttctgtgtaa gggatcaacg gatgcttttc aagataatca
    28381 gtgtactgct cttcactgaa ttctgctaat acggctttgc ccatcgcaga attgtataat
    28441 ggtcgggtga ttccgatttt tgaagacatg cggatcgttt gatgttttgg atctagtttg
    28501 ttcacgtaca agatctcatt gttgtccaat acccctaagt ggatagtctc atcgatcttt
    28561 tgctgcagct gttctaaaaa cggtaaagtc aactctacca aatcgatttg ttcaatgttt
    28621 ttattcgcgt agcgaataag ctttgcacct aaacgatagt ttttctctga atttttattt
    28681 acgtagccga tcatcaacaa agtatctagt attttcaaga ctgtagaaga ggtcattccc
    28741 acaccctttg cgatcgtctg caaagagaca tcttggtttt ccgctaaata gtctagtatt
    28801 ttagaggctt tgatcaatac agttccataa ggctttttat tttccatata atattctcct
    28861 atttctttat aagaaaaatt atagcggaaa taccatcaat ttaaaagaga tatccagata
    28921 taaactacca tttctcttta caggcaattt aatgttgaag atccgcttcg tttttacatc
    28981 aaactttgat ataaagcaat gatttcttct actgtcgttt ctcttggatt tcctggtgtg
    29041 catacatctc gcaaagcatc ttctgcaatt gctggaatat ctgcttcttt tacacctaat
    29101 tcagaaatgg tttctgggat tccaaccgct ttgctcaacc gatcgatctc accacaagcc
    29161 gcttctctta catcttctaa agacatttca gctgctcctt taatccctag aacaagagcg
    29221 atctcacggt acttttctcc agtatattct ttgttatatt tcatgacagt tggcaaaagt
    29281 gctgcacacg ctacgccatg aggaatgttg taccatgcac ttaacgggtg agccatcccg
    29341 tgtaccagtc ctaaaccgac atttgagaag cccattcccg caatatactg tcctaatgcc
    29401 attttttctc gcccattttg atcgccatct acagaaactt ccaaagaatg gccgatgatt
    29461 tcgattgctt ttaaatgcag catatcagtc atttcccaag cgccttttgt gataaaccct
    29521 tcaatagcat gcgtcatagc atccatacct gttgatgcag ccaatttttt cggcattccc
    29581 atcatcatat cgctgtcgat gaacgcgacg attgggatat catgaggatc aacacaaaca
    29641 aattttcggt gatttacttc atctgtgatg acgtagttga tcgttacttc tgctgctgtt
    29701 ccagaagtcg ttggtatagc taatataggt aaacatggat ttttcgtatc agcaacacct
    29761 tctaaactaa tgacgtctga aaactctgga ttcgtcaaaa taatcccaat ggcttttgcc
    29821 gtatcgattg gcgaacctcc accgattgcg atcaaacagt ctgcctctgc ctctttaaca
    29881 aaagccactc catcttttac attttggatc gttggattag gtacgatccc gtcgtataaa
    29941 gcataagcga ttgctgcttt atctagaagt tctgttactt ttgttgctat accgtgctcg
    30001 atcaatcctt tgtcagtaat gacagctgct ttgccgaatc ctcgctgctg aaattcaggg
    30061 acgatcgact caatcgctcc ttttccaaaa tacgatgttt catttaaaat cattcgatta
    30121 ctcattttct attcctccta atttgtattt tttttcacaa taaaaacgaa agaaccccaa
    30181 gcaaccggtt gcccagttgc ttgttgctac agttcaaaga ctggcatcaa ttcccaactg
    30241 actggagagg catctggatt tgtctccatg atatcttcca tgtacttcca ccatttttga
    30301 ttgatagatg tatttgccat tttttcccat aacttttcat cttcgatcac taagtaacca
    30361 aataacaaac ttgtactcgg atcaagatag atcgaatagt cgatgacccc gtgttcacga
    30421 agtgctgtac gcatttctgg ccaaagttca tcatgtcttt ttttgtattc ttcttgtttt
    30481 tctgggtaaa ctttcatgca aaaagctttt ttcaccatgt cagacacctg ctttcgtatg
    30541 aacttcctcc aaataacctg cttttggagt gacaccaaac gcatctgcta aatgccacag
    30601 gttttcgtcc gtgattgttt gcaagattgg tccttgcgct ttgacgtacg tatagacttc
    30661 tgctgccttt tctgctgtct cgatcaaacc aaagacttca tccatgtctt ttcctgaccc
    30721 gtagattcca tgttgtggcc ataaaaccaa tcgagtttct ttcatttttt cagctgttgc
    30781 ttccccaatc tcatttgttc ccggaaccat ccacggaatg atggaaattc cttctggaaa
    30841 aacaactaag cattccgtac acatttgcca tagcgtacga ctaaaggaac gttcgtctaa
    30901 ttcatgcgta aaactcatcg ctaatagatg actagcatgg caatgcataa cgatccgatt
    30961 atctgggtct acttctaaac gggcgatatg actcatgaaa tgacttggca actcgcttgt
    31021 aggaaggcat tcgttttcca gcccccacaa aagttccagt gttttgccat cttcagcaac
    31081 tcgaacgatt ccaagattct cggcaggttc gctcgctaca tttttaaaat atcttcctga
    31141 accagttaca ataaaatagc atcctgctaa acggctggca tcaaaaatca tcgagatgcg
    31201 gcggatcact ttttcaggat ccaagaacgg tgtgatctct tcttctttta gtaaataaga
    31261 aatattcccg ccatttcttt catcccatcc caagcgataa agattcgacg tcgtttcgat
    31321 catttctttc acataaggcg cttgtaaaat atctattttt ttcaatatag ccacactcct
    31381 attttcttaa ttccaacacg tctttttgat attcctggac ttcttccagc caatccaaac
    31441 caactggaac ttgatttttg taacagaaat aattccatac atcggcatac gggaaatctt
    31501 tcaattcttc cgtataagcc attcttgaag taaagtcaaa agccagctcc atttctttca
    31561 atcgctcaac tggttccagc atagctttca gcagtgcttt ttgtgtattt ctcgtaccga
    31621 tgacccaagc agcgatacgg ttgatcgttg catcaaagaa atccagaccg atatttgttt
    31681 tttccaacaa ttcgttacgg actaattctt tagcgatttc ctgcaattca tcatccatca
    31741 aaaccacatg gtctgaatcc caacgcactg gacgggaaac atgcaacatg attcctttgc
    31801 tgaataaagc taatgaagat aatttattgg aaatcacttc tgttggatga aaatgtccag
    31861 catctagaca aacaagtttg tcacgagtca acccgtaacc catataaaat tcatgggaac
    31921 cgactgtata agcttcagcg cctaaaccaa atagtttgct ttctactgca tcttgtacat
    31981 accgttcatc taactcttct gtaaaaattt catcgagtga ttccattaaa cgttcccgtg
    32041 gggtaaaacg atcaatcgga ttatctttca tcccatcagg tacccaaaag ttattgatac
    32101 ttgtttgccc agtttctttc ccaaagtact ccgcgatttt gcggcttctt ttcccgtgtt
    32161 cgatccagta ttcacgaact tctttatctg gatgagccaa tgtaaagccg tctttcatca
    32221 ttggatgcga aaagaaagtc ggattgaagt caagaccgat ttcttctttt tttgcccact
    32281 ctacccattt ttcaaaatgt ttcggctcaa tttcatttag atcgacagat tcttctgtat
    32341 ccaaatagat agcatgaagg ttcaatttgt gtttgcccgg tatcaacgaa taagccttct
    32401 ccagatcttg tctaagctct tcaggtgttc gtgcggctcc tggaaaattc cctgtcgcca
    32461 tgattccacc ggtcaattcg ccatccggtg tcaagaaacc tttgacatca tctccttgcc
    32521 agcaatgcat agaaattttg atgtcctgta tcttttctag tacagcatct gtatctacac
    32581 cgatagttgc atatttttct tttgcttgtt cgtaatttct ttcaatcgtg ttcatacgat
    32641 tactcctttg attttattca cgtaccattc catcgtgttt tgcttatttt catctgggta
    32701 aaaagtttca catggaaacg actgtttgat tacatttctt gcttgttcta tattcgaaaa
    32761 ctgacctgta ctgagcattt gcatggcaac attcccgatt gctgtggctt cactaggtcc
    32821 tgcttctaca atgattccgg cataatctgc tgtcaattga ttcagtagac gattattcga
    32881 tccgccccct acgatgtgca gcttttccaa tttcgttcca gttaagtgtt ctactttgat
    32941 cagctcttgt gcgtaacaaa gagcaagatt atcatacaca caacgagcga tagctcccga
    33001 tgagacaggg actggctgct ctgtttcaaa acagtattgc tgaatctctt ctatcatatt
    33061 tgggggattc aaaaatcgag ggtcattgat ttccatcact tgctgcagcg gtttttcttt
    33121 tgcagcaagc tctgccaatt ccgcataact gtactcgtac tcttgcattc gggcaacttc
    33181 ctggatcaac cacatcccca tgatgttttt caaaaaacgc accgtgttat gggcacccca
    33241 ttcattcgta taattttcag aaaaagcatt gcgagaaaca ttggcgatct ttgtttcaat
    33301 ccctaaaagc gaccatgtcc cactgctgat ataaccccaa cgttctcctt gacctggcgt
    33361 tcctaataca gctgacgccg tatcatgact tgctactgtg ataaaggtca catctggcaa
    33421 atcatatttt gaaaattgtt ctttttgcag atctcctagt atcgttcccg gctctgtcaa
    33481 tggtggaaat aattccttcg agacaccgat tttttcaaga agttctattt cccattcttt
    33541 tgtccctgca ttcagcagct gcatagtaga tgcattagtt ttctccgtca ccatgttacc
    33601 agtaaagaca taccctaaat aatcaggtat cagtaacaat ttatgtgctt ttgccaaatt
    33661 ttctttttct tcagcaaaca attggaacaa ggtattgaaa ggctggatct gaatccctgt
    33721 ctgctcatac aacttatcta gagaatagat ttcagaaaac tgttctactg ctccattggt
    33781 acgcttgtcc cgataagcaa tcggatcatt tatccgatcc ccttgttcat caatcagaca
    33841 gtaatcgact gcccatgtat cgatccccgc aaaaaggcga tcaatccctg tctgctttaa
    33901 tttttcaaat ccaatcaaaa tctctttgat aagataatcg atcttccagc gatcgtgacc
    33961 gtctttgttc cgaaagccgt ttttgaaccg gtgtacttct tccaagatca aagaaccttt
    34021 tgcatccctc gtacttttca ttagtcgtcc gcttgatgca ccgatatcga tggcaacaaa
    34081 tgttggattc atttctccac ctcttttact ttattgttga ttagcacttt attaataaag
    34141 cgttatcatt tattaacaat gctatcataa tacagaatct tatattttgc ttcacactat
    34201 tttggtatat aattgcatta tcttgacaag ttgggagtgt ttacatggat tactgggaaa
    34261 aagccaacca ctcatggtcg gaagactcaa aaagagtgat cttgacgcct acacaaagaa
    34321 gcaggaattt atttttttat atccaagaaa tcggttactt caaagcacca atcccttact
    34381 atactgaacg agcacattta ccttcttact tgataaagtt tactttgtct ggcaaaggtt
    34441 cgcttacata taatgataat cagcaacagc taaaagctgg agacttattt tttattgact
    34501 gccaagaata tcagcgttat caaacgatca gcgaagaacc atgggaaatg gattggatcc
    34561 atttgaatgg tcagaacgtc caatcatttt atcaggagtt cacaaaagac ggccataaca
    34621 ccttccattc aacaggggat ccatcagaaa acaagatcca ccatttgatc acagaattga
    34681 tccacatgca acaaggcacg aatgcccgta ccgatttcca gtcctctgta gctatccacg
    34741 agttgctgaa tgaactgatt ttgcaaaagt atcagctaga tttcgaagaa gaggatattc
    34801 ccgcctacgt ttacaaactg aagcaatact tagacgaaca tttcaaagaa acgattacac
    34861 ttgatcaatt agaacaacaa ttcttattga ataaatatca gctgagcaaa gatttttcca
    34921 aatttatcgg ttctccacca atcgattatt tgatcagcaa aaaactttct tacgctaaag
    34981 accttcttcg gtatacagaa tttacggttc gaatgatttc cgaagaaata gggattgaga
    35041 attttgctta ctttagccgc ctgttcaaag caaaaacagg actatcgccg agagactttc
    35101 gtaaattcag ctgattcata taatatgtac gtcatttttt caaaatattt tgaaatatat
    35161 agataaaaat aaaataaccg cagtttctgc ttctatgtaa acaacagcag ctactgcggt
    35221 tattttttag tgatcattct cattgttcat cttcaaaatg ttcataatga accaactgtt
    35281 tctgtgtatc acgtttcaaa aattctttag gcgttatgcc gaactctctc ttgaatgctt
    35341 tcagaaaatg cggataagaa gaaaaaccac ttttactata tacagcacta gagctgcggt
    35401 attctcgcaa caagtgcttg gcatagatca gctttttctt cattacatac tgatgaaacg
    35461 taaatccagt gtacttttta aattctcgtg tgatatggta tttgctgaca aaaaactgct
    35521 tttccatttc ctctaaagaa agcggatgag ataaattttt tgagatatat tgtaacattt
    35581 gttccacacg ctcattagga actacagcat tctgttcttg cgcactctct tctttttgta
    35641 caacttgatt gagaaaaatc aagtattcta ccagagcttg ttcatatttt atgtcttcac
    35701 cgtaaatttt tttatcagga ggatggtcga taaatgataa tttttgcttc aatacttcct
    35761 gttctatttg caatacacga ctcctcttct ctcccattgg ttgaaaacaa gccgatagat
    35821 tagaccatgg cgtggaacga ctttctagaa aagaaggggt gacaaagata tagacacgct
    35881 cgaatacatc tgaagattgg cggacgatac gatgcaaatc attggaatgg atgagcagca
    35941 cacttcctgc tttgatcgta aattgtcgtc cgttcaaata aaaagtcgct tctcccttta
    36001 atgtagcatg gatctcatag aaatcatgat gatgatagat cgttttattt tggctgacga
    36061 catctttgac atgatagatt tcatactgat cagaaaccat cgtattgact tgttcaccgc
    36121 tgattttctt ttggagcatg tgccgcacct ccttttgttc acttatgcac aattttatta
    36181 taccacgttt tttgtaatca aaaagagctg cttttatata ctcttataaa aaagaagaga
    36241 cgtggcatct tctagccccg ccccctccat tctaaatatt agttaaccaa cgtcttcttt
    36301 acattattca agaagacttc ttccataacg aatcccatca tcttttgttt cttcaagaac
    36361 cacatacagg tacggacgat tcttcatgat gatttctttg atttttgtgt actcgatcac
    36421 accgtctccc aaatttaccg gcacgatttt ttcctgttcg acacgaaaat ctttcaaatg
    36481 aaccgcacat atcttttcac caaaacgttc aaaagcttct tctaccaatt ggacttgttc
    36541 ttgataatca ctacttgtga tcaaattggt cggatctaaa atgattccta gatagtccga
    36601 atctactgct tggatcagct gctctaccct tgctaaagaa tacaccggat gattcaaacc
    36661 tggttctatc cccaccatca tttggtattt ttctccagct tttaccaagc gacggataac
    36721 ggacacagct tcagcaaatg cttcatctgt gaaattttct tccgtatatt ggatttccgg
    36781 caaaacacat ccagtttccg aagcaaccat cgacgcacca aaggaagaag catagcgaag
    36841 atactgctcg aactttgtca gcaactcttg tcgtatcgtt aaatctggat gaatcatatt
    36901 gatatagcaa cttaaaattc ctacggtcac ttcttgcttt tctaaaactc gtttgacata
    36961 atttcccatc ccagaattga tctcatttgc gccagaaggt gtttcaggaa acgaaagacc
    37021 taaggcgagc tgcagtgtat gaatcccttg ttcttttgtt ttttctgcaa gatcttctat
    37081 cgtttggact tcggaaagat catgtcctct aattgtaatg ttcaacataa ctattctcct
    37141 aactcagcat attgggtagc cacatgacga tttgcgggaa gtaagtaatc aaaaacaaga
    37201 cgactaagat aaccccataa aaaggaagaa gcggtttcag cattgcctct gcttttacgc
    37261 ctcctacact tgcaccgaca tatagtcctg tacctactgg cggcgtgatg gagcctacac
    37321 acagattcat gattgaaaac aacccgtaat gtacaggatc aacccctaca cttgtgacga
    37381 ttggtaaaaa tattggtgta aaaatcaaga tagcaggcgc cacatccata aacataccca
    37441 ccagtaataa cacaagattg actaataaca gaataataaa tttattatcc gttacgccca
    37501 taatcaacga actaattgcc tgaggaatac cggtaaacgc catggcgaaa gacatcatgg
    37561 aagaagtagc caataagaac atgatcgtcc ctgacattgc tactgattga aacaacatat
    37621 taggtatatc ttttagtttt accgttttat agtaaaacat agcaatcaac agggagtaaa
    37681 tgacagctat cgctgaagct tctactgctg taaatatccc cgtcaagata ccaccgataa
    37741 tgatgacaat caatagaagg ctaggaatcg cctcttttat tatttttcca gcatgagcca
    37801 tctgtccttt ttcgacaaca gggtatttat tctttttagc aacgatgaat gctactagca
    37861 tgattcctag cgcccacaaa gcgcctacga gataacctcc cataaacaaa gcagagatcg
    37921 aagcaccacc agcaacaagt gaatacatga taaaagcagt acttggagga atgaccatac
    37981 ctgtcggagc tgacgcgata ttgaccgctg tcgcaaactt ccggtcatat ccttcttcca
    38041 cttgctgagg gatcaacact ccgccaatcg cggtagaagc ggcaatggcc gagctagaga
    38101 tggaaccaaa taaagcattc cctaacacat tcgtatgagc caaagccccc ggcacgcgcc
    38161 cgacgaacaa catcgcaaaa tccactagtt tcttagctat ccctccatta ttcatgataa
    38221 tgcccgagag aacgaaaaac ggaatagcga tcagcgaaaa actattgata cttcccacca
    38281 ttttttgagc tgaactaaaa gcagaaacat caaacggtat aactaacaat aaagtcatca
    38341 tcgatgagaa tgcaatactt atagcaatcg gcatccctaa tacaagcaac acaacaaata
    38401 caaaaaataa cactaatccg gcttctattg ccattttatt cctccatttt tatatttttt
    38461 cttgaattgc tggcttgttg cttaatgtat cgattagatt gatcaggcta tagataatga
    38521 tgagtacccc cgatacaggt actgacagat aaacatatcc cataggaata cttaaagaag
    38581 gcgagatctg ccccattgtc aatgtgacag cattccatcc gccatacacc atgatcatcg
    38641 cagcaaatac cagaaataaa ctttgaacaa caacttctaa aattttactt tttttgccag
    38701 ttaatttttc acttaaaacc gttacggcta aatgtgcttt ttgtcccaca acatacgcgg
    38761 aagacaacat ggcaaaccag acaagaccga atcgaacaag ctcttccgtg accgtatttg
    38821 gatttcctaa aaccgttctg gaaaatactt gccataaaac aacagcgacc atcccaacaa
    38881 gcaaaacagc tcccaggatc tctaatacct tgtttaaaat cattttcatc acagctacgc
    38941 tccttgctct tggatctttt gataaaggtc tttgtaggta ggatcgtttt tgaatatatc
    39001 atgaagtggg gcaactgcct ctcgaaacgg tgtattatct acatcattga attggactcc
    39061 atgttcttct tgtgctaaag ctttttcttc ttcaattgct tttgcaaaag cttcaatttc
    39121 aaaaaccgtt gattctttcg ctgcttcttc aatggctttt tcctgctcat cagttagctg
    39181 ttctttgatg gcatcattca tgatgatgat atctggcaca cgtgtatgct catcatagga
    39241 atagtattta gccacaccac catgtccagc tgtatacaag acaaattcat tgttttctga
    39301 tccgttgatc aaattggatt gaagagacgt gtacacttca tcacttccca taggtacggg
    39361 cgaaccgcct aaaagctcta ccattttgat agcggtttcg ctttgcatca cacggatctt
    39421 ctttccctcc aaatcttccg gcgtatggat cggaccgtct accatataaa agctgcgctg
    39481 accagaatca taataggtca atccggtaaa tcctaaattt ttggtcgatt ggtagattgg
    39541 ctccattatt tcttcattat ccatcacctt aaagaaatgt tcttcatcat tgaatagata
    39601 aggaactgaa aaaatcgaat aatctttaga gaaactttcc aacgctgagg cacttacttt
    39661 tgtgaaatct actgctcctg actgtgtcaa ttcgatcagt tcacgttctc cacccagttg
    39721 tccatctggg aaatactgga tctgtacgga accatctgtt ttttctttta ctaaacgccc
    39781 aaattcattc attgcatcaa ttactggctg gctgttgctt gcataagcaa agcgcaaggt
    39841 aactatctca tttacatctg catctgcgac agttgccaca ctagaattat ttccgcagcc
    39901 gcttagacct agcagaccta ggctaagcga acttgctgca aacagcagtg atgttttttt
    39961 catatgggtt tcctttcttt taacaataag agacgagcaa ttctgtcaaa caaagcaccg
    40021 ttaacgactg tccataaggc attgctgtga ttccgatatt ccgataaaaa tctaaactgt
    40081 cccccattcc tgtcccgatt gaaacgtttt gaacttctcc tttttcatcg atttgttcaa
    40141 gcaatccttg gatagcacga taagcgactt ctttatattc ttgtggtaaa taacgtttat
    40201 ggaccgcttt caaaataccg taagcaaatc ctgcagttgc agaagattct acgtaagaag
    40261 aaggatcatc caacaacgta tgccataaac cgctttcaga ctgataagtt tttaatgcac
    40321 gcacttgtgc ttcaagtgtt tcgatcaata atttgcgcaa tgcatcttct ttcgataatt
    40381 ctaagatttc aatgatctct ggaatagcaa tcgtcaacca gcagtttcca cgagcccaaa
    40441 atgcttcggc atagttatga tttccttcaa acgtccagcc gtgataccac aatcctgttc
    40501 gtttatccat caagtatttg atatggatca taaactggta acgagcttct tctaaatatt
    40561 ctgggcgatt caacagtttc ccgatttttg ccaaaggaag cactgtcatc atcaatgtat
    40621 cgtcccacaa ctgattctta ttttccggtc catatgtggc atgctgtaac ccgtcttcat
    40681 ttgtccgagg catgtcattc atgacccatt ctgcccattg ttctaaataa ggcaaatagc
    40741 gtgaatcttt cgtgtcttca tacaagtaag ccatagtcaa tagcggtgcc attgtattga
    40801 cgtttttggg cggagcgcct tcttccatac gatcctcaaa ccattcattg accacttgat
    40861 aagcgcgagg attttttgtc atctggtaat ttttataaat accatataga ccgacacctt
    40921 gtggccaatt ccacactgtc cagcttttgt catctacttt cagtccatca aagtccaaca
    40981 aaaattcacc attatcgtct ttgatcgttg tcaggttttc aacgagcagg tcaatttttt
    41041 ctttcactaa ttctttaggt acaaaaatct cttctttaat aatcatcttt acgccacctt
    41101 tttaaacgct ttcattttat atccttaata tagcagtaat accgtccact ataaatatcg
    41161 ctttttgaac aaaaactgtc taaaattgac ctaaatgtta aataacaaaa gtaactttca
    41221 gttacttgga aattaaaaaa agaacttgga gaaacaacgt tttccaagtt ctttttcatc
    41281 tattttctta tcttttttct acgctttctt ttgcttatcc gttgtaagca tcaatgatga
    41341 tctgtttcaa ttcagagatc aatggttctt tcggattagc tgtcgtacat tggtcttcgt
    41401 aagctaattc agccatacgg tctacggttg tatctagtgt ttcttgtgtg actccttgag
    41461 cttttagact catgttgatg ccgacagatt ttcctaattc ataaacagcc gttgctaaag
    41521 cttcgactaa ttcctctgtt gtttcgcctt tcagacctaa gaattttgcg atatctgcgt
    41581 aatctgtatc tgcacggaaa tagtcatatt tagggaacat cgcatgtttt tgtggatctt
    41641 ttgcattata gcggataata tgcggtaaaa gaatcgcatt tgtccgtcca tgagggatcc
    41701 cgtattcccc gccgatttta tgtgcgactg agtgacaaat acctaggaat gcgttagcaa
    41761 acgccatacc agccattgta gaagcgttgt gcattttttc acgtgattcc atatcaggtg
    41821 tttttactga tttttctaaa tagtcaaaga ctaacttgat tgcttgtagg ctcaatccac
    41881 gtgtataatc tgaagccatg actgaaacat aggattcgat tgcatgtgtt aacacatcca
    41941 tcccggtatc tgctgtgaca gatgctggaa ctgacatgac gaattgagga tcgacgattg
    42001 cgacatctgg cgtcaatgca taatctgcta acggatattt cacatgtgtt tcactgtcag
    42061 tgatgaccgc aaatggcgtt acttcagaac cagtccctga tgttgtcggg atacatacga
    42121 attgtgtttt ttctggtttg tcgatcttat atgtccgttt acggatatcc aagaattttt
    42181 gttttgcacc aaagaaagat gtgtctgggt gttcatagaa catccacatt cccttagctg
    42241 cgtccattgc agaaccgcct ccaagagcga ttactgtatc tggcttgaag tctgccatca
    42301 gtttcgttcc tgcgtatacg gtatttgtcg atgggttcgg ttcaacatct gagaagactt
    42361 cgatcttcac atcattttta cgtcgtgcca atacttcacg aactgtatcg cagtaaccga
    42421 attggaccat accagggtca caaacgatca tgacacgttc aacgttttcc attttctcta
    42481 agtacaatag agagttcttt tcaaagaaga tttttggcgg taatttgaac cattgcatgt
    42541 tatttctccg tttcgctaca gttttgacat tgattaagtt tacagcagag acgttacgag
    42601 aaacagagtt cttaccataa gaaccgcatc ccaaagtcaa tgacggaatc atttcgttat
    42661 agatatcacc gatgccgcct tctgcagatg gtgtattcac taaaatacga caagctttca
    42721 ttcgaagacc gaatttgaca tgcaactctt cgttttctga atggataact gctgtatgtc
    42781 ctaatcctcc aagattcaac ataccttcac acaaatcaaa cgcatggtcc gtgttgttag
    42841 ctttgatcat ggcaagaact ggagaaagtt tttctcttga tagcggataa tctgggccta
    42901 cgccttccaa ttcagcaatc agcattttcg ttccttttgg tacactgatt cctgccaatt
    42961 ttgcgatttc catcgcagaa tgtccaacga ttgatggatt gaccgcatat tttccttcgt
    43021 tcatcactgc atcttctaat ttttgcaatt catctggttt tacgatataa acttggtgtg
    43081 cttggaattc tgcttttact gctgcataga tttctttatc cacgatcaca gcttgttcag
    43141 aagcacaaat cattccatta tcaaatgttt tcgaaacgat caaatcgttc actgcacgtt
    43201 taatttttgc tgttttttca atataagctg gtacgttacc tggacctaca cctaaagctg
    43261 gttttcctgt agagtaagca gacttgacca ttccagctcc acctgtcgct aagacgatcg
    43321 cgatacctgg atggttcatt agcatagaag tcgcttcgat cgaaggatgt tcgatccatt
    43381 ggatacaatt ttcaggagct ccggcagcga tagcagcatc acgtaccact cgtgctgctt
    43441 ctgcagaaga tttttgggca cttggatgga aagcaaaaac gattggattt cttgttttca
    43501 acgcgatcat tgatttgaaa atcgttgtgg aagtcggatt cgttgttgga gttactccgc
    43561 aaaccacacc gactggttca gcaatttcaa tcaacccttt ttggacatct tcattgatca
    43621 caccaactgt tttatcatgt ttgatattat gccaaatata ttcggacgca tacatatttt
    43681 tgattgcttt atcttcgtaa acaccgcggc ctgtttcctc tacagccatc ttcgctaatg
    43741 gcatatgttg atccaaagct gccattgcca tttcatgcac gatatggtct actttttctt
    43801 gatcaaagct ttccatttct ttcaatgctg catttgcttt ttctgccaaa tcatcaatca
    43861 ttgcttgtac gtcaattgtc ttcgtttttt ctttttctat tactttagcc atcattatcc
    43921 tcctaatggg tcatggttat ttgttaaata attcacatgt atatcataca atatttattt
    43981 ttctatgtaa acactttttt gtgatttttt tcacagttat ttttaaatat aaaattgctt
    44041 tatttacagg tgtttgtata gaaaaatata taaagttatt tattattatt tatatgacac
    44101 acccgattat tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata
    44161 aaaaatgagg taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat
    44221 ttttcttcct tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact
    44281 gtctcagctg gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc
    44341 cctggtttga ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct
    44401 aaaacaactg tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc
    44461 gttacgactg aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt
    44521 ttcttttgcg aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc
    44581 attggtaatg ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag
    44641 cagaaaaatg ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag
    44701 ctttttgcgt tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc
    44761 aaattatcat ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa
    44821 ttgtggtaag aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata
    44881 taggcacgtg tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa
    44941 tcatgggcat atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca
    45001 tttcgagcga ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca
    45061 tcgatcaatg aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc
    45121 aacggtgtag tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac
    45181 ccgccgattg agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga
    45241 agatcttcaa accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca
    45301 tgagctttca atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag
    45361 tcgtaacttt catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat
    45421 ttattttgga tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga
    45481 tttttgaaat gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga
    45541 aagccgcctg aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca
    45601 gcttcttcta ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata
    45661 acgccagcac ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt
    45721 cctactggca tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa
    45781 cgcgcgccag tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt
    45841 catttcccta ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag
    45901 tgctttttgc aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct
    45961 ttttttatga ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg
    46021 tatattggtg ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa
    46081 cgccaagcaa aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc
    46141 cctacattga ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc
    46201 accaccatat atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt
    46261 tcaggaggcg gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt
    46321 tcttggactt atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat
    46381 gcatttcgtg gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc
    46441 tcgatcttta caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat
    46501 tcttattctc aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct
    46561 aaagtactgg atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt
    46621 ttctggttag atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt
    46681 ggttatctat ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg
    46741 ccaaaagata tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt
    46801 ttatcgagag gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat
    46861 tcatgtcaca cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt
    46921 aatcattcct cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg
    46981 attggtacga tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg
    47041 ccgaatagtt cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt
    47101 aacgccattt ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg
    47161 ctgcgtacat tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt
    47221 tgaccgacat gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa
    47281 taataaccaa acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat
    47341 aaacctcgag caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt
    47401 tccgctggct ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac
    47461 agtagttgca cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata
    47521 aagatctccg aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa
    47581 accgaacgtc cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc
    47641 atatctgcgg cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta
    47701 cttgctttca acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc
    47761 gtacgttgcc aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca
    47821 gagagagctt tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct
    47881 gaaagtgctt ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc
    47941 accacatgat ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt
    48001 gctcctttac tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag
    48061 tctttttcca gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca
    48121 acagcaatca ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct
    48181 acatcgcctg aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg
    48241 tatttttcgc ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc
    48301 tcgtttactc gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca
    48361 gcccttacga tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc
    48421 tcgaccgtca catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc
    48481 attgaatctt cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt
    48541 tcattttgtg tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt
    48601 tcaactgctg gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat
    48661 gcaagtgtca acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa
    48721 atcgaagtca tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact
    48781 aaagccgcag ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc
    48841 tgtaaaggtg ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct
    48901 gtagccatgc caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct
    48961 ttaaatacca tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca
    49021 tctttttcaa ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat
    49081 gcttcgatca aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca
    49141 cccactacca gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc
    49201 gtttgtttat tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca
    49261 atcgccaata ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc
    49321 ccatgttctg tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc
    49381 gttgtaaaat cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca
    49441 tcgaatttat tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca
    49501 gtatctggat ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc
    49561 atcctattcc tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc
    49621 tttttttatt tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt
    49681 aaagtgctta tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag
    49741 gataccatca aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt
    49801 agaagtacag gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg
    49861 tatggatgtt tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga
    49921 ataacgtaaa cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta
    49981 acgaaaacaa aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat
    50041 ggaaagttac aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt
    50101 atcgagagct cggcttttt
//
LOCUS       DACDHL010000013        44702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDHL010000013 DACDHL010000000
VERSION     DACDHL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.013"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1008)
                     /locus_tag="IV228_000779"
     CDS             complement(<1..1008)
                     /locus_tag="IV228_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8716717.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1188..1532)
                     /locus_tag="IV228_000780"
     CDS             complement(1188..1532)
                     /locus_tag="IV228_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8716718.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1525..1959)
                     /locus_tag="IV228_000781"
     CDS             complement(1525..1959)
                     /locus_tag="IV228_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8716719.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2097..2834
                     /locus_tag="IV228_000782"
     CDS             2097..2834
                     /locus_tag="IV228_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716720.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2827..4038
                     /locus_tag="IV228_000783"
     CDS             2827..4038
                     /locus_tag="IV228_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716721.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4245..5024
                     /locus_tag="IV228_000784"
     CDS             4245..5024
                     /locus_tag="IV228_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8716722.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5094..5756
                     /gene="trmB"
                     /locus_tag="IV228_000785"
     CDS             5094..5756
                     /gene="trmB"
                     /locus_tag="IV228_000785"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8716723.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5854..7608)
                     /gene="ade"
                     /locus_tag="IV228_000786"
     CDS             complement(5854..7608)
                     /gene="ade"
                     /locus_tag="IV228_000786"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8716724.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7740..8669
                     /gene="cpaF"
                     /locus_tag="IV228_000787"
     CDS             7740..8669
                     /gene="cpaF"
                     /locus_tag="IV228_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8716725.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8626..9675
                     /locus_tag="IV228_000788"
     CDS             8626..9675
                     /locus_tag="IV228_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8716726.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9672..9950
                     /locus_tag="IV228_000789"
     CDS             9672..9950
                     /locus_tag="IV228_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8716727.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9925..10395
                     /locus_tag="IV228_000790"
     CDS             9925..10395
                     /locus_tag="IV228_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8716728.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10412..10714
                     /locus_tag="IV228_000791"
     CDS             10412..10714
                     /locus_tag="IV228_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716729.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10731..11102
                     /locus_tag="IV228_000792"
     CDS             10731..11102
                     /locus_tag="IV228_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8716730.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11074..11433
                     /locus_tag="IV228_000793"
     CDS             11074..11433
                     /locus_tag="IV228_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716731.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11605..12612
                     /locus_tag="IV228_000794"
     CDS             11605..12612
                     /locus_tag="IV228_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8716732.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12637..13821
                     /locus_tag="IV228_000795"
     CDS             12637..13821
                     /locus_tag="IV228_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8716733.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14258..15841)
                     /locus_tag="IV228_000796"
     CDS             complement(14258..15841)
                     /locus_tag="IV228_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8716734.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16419..16562)
                     /locus_tag="IV228_000797"
     CDS             complement(16419..16562)
                     /locus_tag="IV228_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8716735.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16785..17264)
                     /locus_tag="IV228_000798"
     CDS             complement(16785..17264)
                     /locus_tag="IV228_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8716736.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17337..17621)
                     /locus_tag="IV228_000799"
     CDS             complement(17337..17621)
                     /locus_tag="IV228_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716737.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17699..17893)
                     /gene="ssrS"
                     /locus_tag="IV228_000800"
     ncRNA           complement(17699..17893)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV228_000800"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17965..19251)
                     /locus_tag="IV228_000801"
     CDS             complement(17965..19251)
                     /locus_tag="IV228_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8716738.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19389..20060
                     /locus_tag="IV228_000802"
     CDS             19389..20060
                     /locus_tag="IV228_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8716739.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20026..20514
                     /locus_tag="IV228_000803"
     CDS             20026..20514
                     /locus_tag="IV228_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8716740.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20527..21474
                     /gene="prmA"
                     /locus_tag="IV228_000804"
     CDS             20527..21474
                     /gene="prmA"
                     /locus_tag="IV228_000804"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8716741.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21475..22239
                     /locus_tag="IV228_000805"
     CDS             21475..22239
                     /locus_tag="IV228_000805"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8716742.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22472..24685
                     /locus_tag="IV228_000806"
     CDS             22472..24685
                     /locus_tag="IV228_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8716743.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24696..25142
                     /locus_tag="IV228_000807"
     CDS             24696..25142
                     /locus_tag="IV228_000807"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8716744.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25313..26572
                     /locus_tag="IV228_000808"
     CDS             25313..26572
                     /locus_tag="IV228_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8716745.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26661..27608)
                     /locus_tag="IV228_000809"
     CDS             complement(26661..27608)
                     /locus_tag="IV228_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8716746.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28002..29294
                     /locus_tag="IV228_000810"
     CDS             28002..29294
                     /locus_tag="IV228_000810"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8716747.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29309..31078
                     /gene="aspS"
                     /locus_tag="IV228_000811"
     CDS             29309..31078
                     /gene="aspS"
                     /locus_tag="IV228_000811"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8716748.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31187..32062
                     /locus_tag="IV228_000812"
     CDS             31187..32062
                     /locus_tag="IV228_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8716749.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32328..34211
                     /locus_tag="IV228_000813"
     CDS             32328..34211
                     /locus_tag="IV228_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8716750.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34286..34570)
                     /locus_tag="IV228_000814"
     CDS             complement(34286..34570)
                     /locus_tag="IV228_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8716751.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34560..35294)
                     /locus_tag="IV228_000815"
     CDS             complement(34560..35294)
                     /locus_tag="IV228_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8716752.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35423..36046
                     /locus_tag="IV228_000816"
     CDS             35423..36046
                     /locus_tag="IV228_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8716753.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36238..37359
                     /locus_tag="IV228_000817"
     CDS             36238..37359
                     /locus_tag="IV228_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8716754.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37366..40494
                     /locus_tag="IV228_000818"
     CDS             37366..40494
                     /locus_tag="IV228_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8716755.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40513..43707
                     /locus_tag="IV228_000819"
     CDS             40513..43707
                     /locus_tag="IV228_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8716756.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43853..44560)
                     /locus_tag="IV228_000820"
     CDS             complement(43853..44560)
                     /locus_tag="IV228_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8716757.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14339 a         7947 c         9388 g        13028 t
ORIGIN      
        1 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
       61 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
      121 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
      181 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
      241 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
      301 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
      361 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
      421 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
      481 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
      541 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
      601 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
      661 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
      721 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
      781 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
      841 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
      901 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
      961 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     1021 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     1081 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     1141 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     1201 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     1261 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     1321 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     1381 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     1441 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     1501 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     1561 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     1621 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     1681 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     1741 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     1801 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     1861 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     1921 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     1981 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     2041 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     2101 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     2161 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     2221 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     2281 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     2341 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     2401 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     2461 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     2521 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     2581 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     2641 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     2701 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     2761 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     2821 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     2881 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     2941 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     3001 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     3061 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     3121 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     3181 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     3241 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     3301 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     3361 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     3421 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     3481 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     3541 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     3601 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     3661 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     3721 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     3781 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     3841 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     3901 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     3961 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     4021 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     4081 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     4141 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     4201 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     4261 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     4321 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     4381 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     4441 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     4501 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     4561 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     4621 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     4681 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     4741 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     4801 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     4861 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     4921 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     4981 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     5041 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     5101 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     5161 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     5221 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     5281 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     5341 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     5401 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     5461 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     5521 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     5581 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     5641 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     5701 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     5761 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     5821 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     5881 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     5941 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     6001 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     6061 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     6121 attgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     6181 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     6241 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     6301 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     6361 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     6421 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     6481 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     6541 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     6601 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     6661 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     6721 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     6781 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     6841 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     6901 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     6961 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     7021 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     7081 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     7141 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     7201 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     7261 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     7321 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     7381 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     7441 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     7501 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     7561 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     7621 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     7681 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     7741 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     7801 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     7861 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     7921 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     7981 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     8041 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     8101 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     8161 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     8221 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     8281 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     8341 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     8401 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     8461 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     8521 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     8581 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     8641 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     8701 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     8761 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     8821 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     8881 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     8941 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     9001 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     9061 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     9121 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     9181 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     9241 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     9301 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     9361 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     9421 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     9481 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     9541 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     9601 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     9661 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     9721 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     9781 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     9841 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     9901 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     9961 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    10021 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    10081 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    10141 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    10201 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    10261 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    10321 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    10381 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    10441 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    10501 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    10561 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    10621 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    10681 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    10741 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    10801 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    10861 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    10921 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    10981 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    11041 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    11101 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    11161 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    11221 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    11281 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    11341 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    11401 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    11461 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    11521 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    11581 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    11641 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    11701 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    11761 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    11821 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    11881 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    11941 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    12001 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    12061 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    12121 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    12181 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    12241 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    12301 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    12361 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    12421 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    12481 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    12541 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    12601 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    12661 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    12721 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    12781 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    12841 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    12901 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    12961 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    13021 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    13081 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    13141 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    13201 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    13261 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    13321 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    13381 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    13441 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    13501 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    13561 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    13621 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    13681 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    13741 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    13801 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    13861 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    13921 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    13981 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    14041 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    14101 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    14161 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    14221 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    14281 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    14341 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    14401 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    14461 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    14521 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    14581 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    14641 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    14701 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    14761 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    14821 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    14881 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    14941 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    15001 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    15061 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    15121 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    15181 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    15241 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    15301 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    15361 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    15421 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    15481 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    15541 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    15601 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    15661 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    15721 tgccagttcc gttgataccg actaaggcaa ttttttcacc agcttcaatt tgacaggata
    15781 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    15841 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    15901 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    15961 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    16021 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    16081 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    16141 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    16201 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    16261 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    16321 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    16381 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    16441 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    16501 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    16561 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    16621 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    16681 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    16741 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    16801 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    16861 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    16921 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    16981 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    17041 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    17101 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    17161 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    17221 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    17281 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    17341 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    17401 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    17461 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    17521 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    17581 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    17641 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    17701 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    17761 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    17821 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    17881 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    17941 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    18001 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    18061 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    18121 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    18181 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    18241 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    18301 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    18361 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    18421 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    18481 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    18541 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    18601 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    18661 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    18721 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    18781 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    18841 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    18901 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    18961 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    19021 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    19081 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    19141 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    19201 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    19261 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    19321 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    19381 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    19441 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    19501 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    19561 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    19621 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    19681 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    19741 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    19801 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    19861 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    19921 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    19981 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    20041 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    20101 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    20161 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    20221 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    20281 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    20341 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    20401 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    20461 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    20521 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    20581 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    20641 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    20701 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    20761 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    20821 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    20881 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    20941 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    21001 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    21061 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    21121 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    21181 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    21241 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    21301 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    21361 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    21421 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    21481 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    21541 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    21601 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    21661 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    21721 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    21781 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    21841 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    21901 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    21961 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    22021 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    22081 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    22141 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    22201 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    22261 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    22321 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    22381 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    22441 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    22501 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    22561 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    22621 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    22681 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    22741 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    22801 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    22861 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    22921 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    22981 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    23041 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    23101 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    23161 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    23221 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    23281 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    23341 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    23401 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    23461 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    23521 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    23581 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    23641 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    23701 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    23761 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    23821 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    23881 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    23941 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    24001 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    24061 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    24121 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    24181 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    24241 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    24301 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    24361 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    24421 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    24481 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    24541 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    24601 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    24661 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    24721 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    24781 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    24841 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    24901 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    24961 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    25021 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    25081 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    25141 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    25201 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    25261 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    25321 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    25381 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    25441 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    25501 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    25561 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    25621 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    25681 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    25741 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    25801 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    25861 aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    25921 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    25981 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    26041 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    26101 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    26161 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    26221 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    26281 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    26341 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    26401 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    26461 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    26521 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    26581 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    26641 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    26701 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    26761 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    26821 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    26881 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    26941 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    27001 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    27061 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    27121 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    27181 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    27241 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    27301 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    27361 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    27421 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    27481 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    27541 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    27601 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    27661 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    27721 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    27781 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    27841 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    27901 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    27961 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    28021 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    28081 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    28141 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    28201 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    28261 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    28321 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    28381 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    28441 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    28501 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    28561 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    28621 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    28681 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    28741 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    28801 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    28861 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    28921 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    28981 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    29041 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    29101 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    29161 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    29221 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    29281 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    29341 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    29401 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    29461 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    29521 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    29581 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    29641 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    29701 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    29761 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    29821 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    29881 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    29941 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    30001 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    30061 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    30121 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    30181 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    30241 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    30301 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    30361 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    30421 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    30481 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    30541 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    30601 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    30661 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    30721 tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt ctatccgtat
    30781 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    30841 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    30901 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    30961 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    31021 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    31081 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    31141 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    31201 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    31261 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    31321 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    31381 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    31441 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    31501 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    31561 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    31621 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    31681 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    31741 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    31801 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    31861 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    31921 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    31981 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    32041 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    32101 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    32161 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    32221 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    32281 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    32341 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    32401 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    32461 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    32521 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    32581 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    32641 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    32701 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    32761 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    32821 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    32881 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    32941 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    33001 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    33061 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    33121 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    33181 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    33241 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    33301 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    33361 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    33421 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    33481 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    33541 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    33601 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    33661 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    33721 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    33781 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    33841 taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg aatggctcat
    33901 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    33961 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    34021 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    34081 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    34141 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    34201 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    34261 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    34321 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    34381 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    34441 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    34501 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    34561 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    34621 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    34681 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    34741 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    34801 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    34861 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    34921 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    34981 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    35041 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    35101 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    35161 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    35221 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    35281 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    35341 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    35401 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    35461 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    35521 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    35581 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    35641 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    35701 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    35761 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    35821 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    35881 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    35941 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    36001 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    36061 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    36121 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    36181 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    36241 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    36301 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    36361 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    36421 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    36481 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    36541 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    36601 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    36661 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    36721 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    36781 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    36841 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    36901 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    36961 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    37021 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    37081 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    37141 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    37201 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    37261 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    37321 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    37381 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    37441 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    37501 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    37561 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    37621 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    37681 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    37741 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    37801 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    37861 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    37921 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    37981 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    38041 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    38101 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    38161 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    38221 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    38281 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    38341 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    38401 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    38461 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    38521 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    38581 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    38641 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    38701 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    38761 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    38821 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    38881 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    38941 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    39001 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    39061 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    39121 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    39181 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    39241 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    39301 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    39361 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    39421 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    39481 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    39541 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    39601 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    39661 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    39721 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    39781 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    39841 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    39901 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    39961 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    40021 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    40081 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    40141 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    40201 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    40261 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    40321 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    40381 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    40441 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    40501 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    40561 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    40621 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    40681 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    40741 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    40801 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    40861 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    40921 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    40981 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    41041 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    41101 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    41161 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    41221 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    41281 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    41341 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    41401 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    41461 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    41521 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    41581 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    41641 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggc
    41701 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    41761 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    41821 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    41881 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    41941 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    42001 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    42061 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    42121 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    42181 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    42241 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    42301 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    42361 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    42421 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    42481 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    42541 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    42601 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    42661 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    42721 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    42781 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    42841 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    42901 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    42961 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    43021 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    43081 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    43141 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    43201 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    43261 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    43321 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    43381 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    43441 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    43501 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    43561 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    43621 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    43681 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    43741 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    43801 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    43861 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    43921 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    43981 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    44041 tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    44101 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    44161 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    44221 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    44281 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    44341 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    44401 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    44461 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    44521 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    44581 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    44641 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    44701 tt
//
LOCUS       DACDHL010000014        43920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDHL010000014 DACDHL010000000
VERSION     DACDHL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.014"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(127..504)
                     /locus_tag="IV228_000821"
     CDS             complement(127..504)
                     /locus_tag="IV228_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716758.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            760..1488
                     /locus_tag="IV228_000822"
     CDS             760..1488
                     /locus_tag="IV228_000822"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8716759.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1488..1742
                     /gene="purS"
                     /locus_tag="IV228_000823"
     CDS             1488..1742
                     /gene="purS"
                     /locus_tag="IV228_000823"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8716760.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1744..2415
                     /gene="purQ"
                     /locus_tag="IV228_000824"
     CDS             1744..2415
                     /gene="purQ"
                     /locus_tag="IV228_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8716761.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2416..4638
                     /gene="purL"
                     /locus_tag="IV228_000825"
     CDS             2416..4638
                     /gene="purL"
                     /locus_tag="IV228_000825"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8716762.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4623..6062
                     /locus_tag="IV228_000826"
     CDS             4623..6062
                     /locus_tag="IV228_000826"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8716763.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6094..7137
                     /locus_tag="IV228_000827"
     CDS             6094..7137
                     /locus_tag="IV228_000827"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8716764.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7134..7712
                     /gene="purN"
                     /locus_tag="IV228_000828"
     CDS             7134..7712
                     /gene="purN"
                     /locus_tag="IV228_000828"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8716765.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7709..9244
                     /gene="purH"
                     /locus_tag="IV228_000829"
     CDS             7709..9244
                     /gene="purH"
                     /locus_tag="IV228_000829"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8716766.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9260..10534
                     /gene="purD"
                     /locus_tag="IV228_000830"
     CDS             9260..10534
                     /gene="purD"
                     /locus_tag="IV228_000830"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8716767.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10656..11570
                     /locus_tag="IV228_000831"
     CDS             10656..11570
                     /locus_tag="IV228_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8716768.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11703..13268)
                     /locus_tag="IV228_000832"
     CDS             complement(11703..13268)
                     /locus_tag="IV228_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8716769.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13261..14712)
                     /locus_tag="IV228_000833"
     CDS             complement(13261..14712)
                     /locus_tag="IV228_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8716770.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14726..15742)
                     /locus_tag="IV228_000834"
     CDS             complement(14726..15742)
                     /locus_tag="IV228_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8716771.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15962..17050
                     /locus_tag="IV228_000835"
     CDS             15962..17050
                     /locus_tag="IV228_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8716772.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17104..18630
                     /locus_tag="IV228_000836"
     CDS             17104..18630
                     /locus_tag="IV228_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8716773.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18632..19837
                     /locus_tag="IV228_000837"
     CDS             18632..19837
                     /locus_tag="IV228_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8716774.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20094..21071
                     /gene="guaC"
                     /locus_tag="IV228_000838"
     CDS             20094..21071
                     /gene="guaC"
                     /locus_tag="IV228_000838"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8716775.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21335..22759
                     /locus_tag="IV228_000839"
     CDS             21335..22759
                     /locus_tag="IV228_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8716776.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23220..24329
                     /gene="pdhA"
                     /locus_tag="IV228_000840"
     CDS             23220..24329
                     /gene="pdhA"
                     /locus_tag="IV228_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8716777.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24332..25309
                     /locus_tag="IV228_000841"
     CDS             24332..25309
                     /locus_tag="IV228_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8716778.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25334..26977
                     /locus_tag="IV228_000842"
     CDS             25334..26977
                     /locus_tag="IV228_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8716779.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26985..28391
                     /gene="lpdA"
                     /locus_tag="IV228_000843"
     CDS             26985..28391
                     /gene="lpdA"
                     /locus_tag="IV228_000843"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8716780.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28553..28828
                     /locus_tag="IV228_000844"
     CDS             28553..28828
                     /locus_tag="IV228_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8716781.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28834..29601
                     /locus_tag="IV228_000845"
     CDS             28834..29601
                     /locus_tag="IV228_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8716782.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29769..31598
                     /gene="typA"
                     /locus_tag="IV228_000846"
     CDS             29769..31598
                     /gene="typA"
                     /locus_tag="IV228_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8716783.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31859..33631
                     /gene="recQ"
                     /locus_tag="IV228_000847"
     CDS             31859..33631
                     /gene="recQ"
                     /locus_tag="IV228_000847"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8716784.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33745..34047
                     /locus_tag="IV228_000848"
     CDS             33745..34047
                     /locus_tag="IV228_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8716785.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34050..35213
                     /locus_tag="IV228_000849"
     CDS             34050..35213
                     /locus_tag="IV228_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8716786.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35239..38667
                     /locus_tag="IV228_000850"
     CDS             35239..38667
                     /locus_tag="IV228_000850"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8716787.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38688..39812
                     /locus_tag="IV228_000851"
     CDS             38688..39812
                     /locus_tag="IV228_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716788.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39802..40239
                     /locus_tag="IV228_000852"
     CDS             39802..40239
                     /locus_tag="IV228_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8716789.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40223..40561
                     /locus_tag="IV228_000853"
     CDS             40223..40561
                     /locus_tag="IV228_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8716790.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40680..41240
                     /gene="rsmD"
                     /locus_tag="IV228_000854"
     CDS             40680..41240
                     /gene="rsmD"
                     /locus_tag="IV228_000854"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8716791.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41237..41728
                     /gene="coaD"
                     /locus_tag="IV228_000855"
     CDS             41237..41728
                     /gene="coaD"
                     /locus_tag="IV228_000855"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8716792.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41718..42761
                     /locus_tag="IV228_000856"
     CDS             41718..42761
                     /locus_tag="IV228_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8716793.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(42862..>43920)
                     /locus_tag="IV228_000857"
     CDS             complement(42862..>43920)
                     /locus_tag="IV228_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAP8716794.1"
                     /translation="KLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILM
                     ELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGS
                     MSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTV
                     FPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDST
                     TLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQET
                     LKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRN
                     FYNFRARIYLQQGLIFEN"
BASE COUNT        14431 a         7554 c         9803 g        12132 t
ORIGIN      
        1 aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc ttatcaataa
       61 taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat acaaaaaata
      121 gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat ggagatgttg
      181 cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga tctggatacg
      241 ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt cgcctttgtg
      301 ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg ttgctcggcg
      361 gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt ctttgatcga
      421 cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata aaaaaagctg
      481 accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta tccaaatttt
      541 tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga tggtataaga
      601 ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat gtacatattt
      661 ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa caaaaaacga
      721 tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa agaacttttg
      781 tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat ttggatagaa
      841 tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag tggaaaagga
      901 gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag aggaataaaa
      961 aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt acaaattatc
     1021 cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt
     1081 cccgaaggta aagaattcat caaaccaatc attgaattct actataagga tgaccagttg
     1141 gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa gccggaagaa
     1201 atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga attatttgct
     1261 aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa tggacaaatt
     1321 ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg tacgaatgaa
     1381 catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa
     1441 gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg tatttcgtaa
     1501 aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca gtaaaaaaag
     1561 cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa tactttgaga
     1621 tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc
     1681 tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg gaggaagtat
     1741 aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg
     1801 gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga gtcctcttta
     1861 gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta tttgagatgc
     1921 ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg
     1981 aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg acttttgcca
     2041 ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc attgaaagtc
     2101 aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta tcttccagtt
     2161 gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat
     2221 aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa cattgcaggg
     2281 attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg tgcagtagaa
     2341 gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa aaacttcgga
     2401 aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat caagaaaaaa
     2461 agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga agaagatatc
     2521 ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg gagcgaacat
     2581 tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg acctcatgtg
     2641 ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact agcagttgtc
     2701 ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg agctgcgaca
     2761 ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat tgcgttgcta
     2821 gattcattga ggttcggcga attagataat ccaagaacga aatatttact agaagaagta
     2881 gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg cggcgaaatt
     2941 gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt
     3001 gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat catgtatgta
     3061 ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga agaatttaat
     3121 caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat ggaaaagctg
     3181 ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat aggtatacag
     3241 gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa agctggctca
     3301 ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac accttatgaa
     3361 atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg agaggaagaa
     3421 aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg taaagtaaca
     3481 gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct gccagtcgat
     3541 gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc tcgaatcgta
     3601 gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca agtattgctt
     3661 gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac ttatgattca
     3721 caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga
     3781 gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct gtatttggat
     3841 ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat tgctagcggt
     3901 ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa accagaagtt
     3961 ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac gttaggaact
     4021 cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc aatctatcca
     4081 acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac tactcaggaa
     4141 ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga ttttgatggc
     4201 agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta
     4261 tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg attgattgaa
     4321 agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc agctttcaaa
     4381 catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt tgcagaaact
     4441 caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga agaaatgatg
     4501 ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga aatttcagca
     4561 actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga ggatgcaatc
     4621 gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt gtttggggtc
     4681 atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat cgaggacaag
     4741 aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga gatttggggc
     4801 tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga gaagctgcaa
     4861 tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt cagccttttc
     4921 tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg atcaatgctc
     4981 gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac tccgatacgg
     5041 agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa
     5101 gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa acggcaatga
     5161 ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg aaaaacggag
     5221 catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt tttataagag
     5281 atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt gattgcttta
     5341 cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc agaccagatt
     5401 caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg aatttagcga
     5461 aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct ttgtcagccg
     5521 caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta aaaaatcaat
     5581 acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt gttcgaatga
     5641 aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc gatgattcaa
     5701 ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca ggggcaaaag
     5761 aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac gggattgata
     5821 ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc aaagaatgta
     5881 tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg atcggtctgg
     5941 aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat tatccgacgc
     6001 cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta tcgttctttt
     6061 aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc aaaagctggc
     6121 gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt caaaaaaacg
     6181 gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga tttatcggtc
     6241 tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac gaaattagca
     6301 gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc gatgtgtgtc
     6361 aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt agcaacgggg
     6421 aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa
     6481 ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat
     6541 gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt attgacagga
     6601 gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat tcattcaaat
     6661 gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt atcatcaaaa
     6721 ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa aatctatgtt
     6781 tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca tatcactggt
     6841 ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa
     6901 cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg tgaaattcct
     6961 gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc agtccaagaa
     7021 tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc
     7081 gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga acgatgagaa
     7141 tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat tatctatcga
     7201 aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa gcatatgttt
     7261 tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa gaatttgatt
     7321 caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag atcgatttga
     7381 tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat tatgataaaa
     7441 gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat ggcatacgtg
     7501 atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg
     7561 tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag gacacgttcg
     7621 attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa gtgattagtg
     7681 aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg tatcagataa
     7741 aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa tcatttcaac
     7801 aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga tcgaagaagt
     7861 gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta aaatccatgg
     7921 tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa cggaaaatat
     7981 ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga ttatgaaacc
     8041 agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta gtatgctccg
     8101 tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg actatgaaga
     8161 agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac aaaaactagc
     8221 ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat acttaacgga
     8281 ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa aacaaacgct
     8341 tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc ttctggaaaa
     8401 atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata ataatatccg
     8461 agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt
     8521 gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag cgtgggaagc
     8581 agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga atcgagaagt
     8641 agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca ttgccccttc
     8701 ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat tactgacagt
     8761 agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct taggtggact
     8821 tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa
     8881 acgtaagccg actccagcag aagcaaaagc attagcgttt gcttggagag ctgtcaaaca
     8941 tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa tcggagccgg
     9001 gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga
     9061 aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg aatatgcagc
     9121 caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc aagattctat
     9181 tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc attttagaca
     9241 ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc
     9301 aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa
     9361 tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag atcattcagc
     9421 gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc ctgaagttcc
     9481 tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct tcggtccgac
     9541 aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa tggatactta
     9601 tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag cagcgtatat
     9661 caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt
     9721 agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt tgaaccagca
     9781 tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag gagaagaatt
     9841 ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt cgcaagatca
     9901 taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg cttattcacc
     9961 agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt taaaaccagc
    10021 ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg cgggtttgat
    10081 tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg atcctgaaac
    10141 gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg atttattaga
    10201 gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg ttgtggttgc
    10261 agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt ttctggaaga
    10321 agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag ttggagcagg
    10381 aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac agaaaaaaat
    10441 ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag atattggaga
    10501 aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat
    10561 acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag agtgtggtac
    10621 acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa ttaggtactt
    10681 ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat gcggaagatc
    10741 cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac catgcagata
    10801 tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa acatcaatca
    10861 aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt
    10921 tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg aaaatggatt
    10981 atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag gaagtagctg
    11041 aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa
    11101 aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta gctaatcagc
    11161 tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc aatatgacag
    11221 atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg catgatatga
    11281 cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt atcgggaacg
    11341 aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat caaacgacac
    11401 caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa gtgatcgccg
    11461 gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc
    11521 gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa agattgtcat
    11581 aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct gttttcagct
    11641 tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac ggatttcgtt
    11701 tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg agaaatccct
    11761 tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat ttcaaaagca
    11821 atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc gattctttgt
    11881 tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt actgaaatga
    11941 gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg atcggtaaaa
    12001 ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt ccttgaaggc
    12061 tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag
    12121 tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg
    12181 attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa taaacgatat
    12241 aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta cttagatact
    12301 tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac agaatcaact
    12361 ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt aagggaactg
    12421 gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct gattctttct
    12481 gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa ttcatggacc
    12541 aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga atcaactatt
    12601 aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt ctccctaaat
    12661 aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact gacttggttt
    12721 gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga ttcagcaagt
    12781 ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa atgatttttt
    12841 ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa atctaaagca
    12901 gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt aagcagataa
    12961 tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa tccactaata
    13021 aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc
    13081 atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat aattgaagac
    13141 agtgccatct caccatctcc agcttcgccg acataagtga ttggataata agaaccaagg
    13201 acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat gataaaaact
    13261 ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca gtgtgacagt
    13321 tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc cgtaataaag
    13381 aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc
    13441 tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa tcccgttgtc
    13501 tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa taaatccttt
    13561 gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct gcaagatcat
    13621 ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt caagcatatg
    13681 gccgtatctt atttttaaga tgactagata atcattgata tggctaattt ctttttcaag
    13741 aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt
    13801 catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg catccaaact
    13861 gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac tttgtgctaa
    13921 atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg ccattttatt
    13981 catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac gaaagccaag
    14041 gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt cttggacctt
    14101 tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa gaactccaag
    14161 ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa
    14221 gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag tgacagaatc
    14281 tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga tattattgat
    14341 cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat ctaacgtact
    14401 aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc taagttcttc
    14461 taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca agtcccacat
    14521 actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt ggatatttgc
    14581 gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca gtagcaaagg
    14641 gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa
    14701 attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta gctatattct
    14761 gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt cctgtttgaa
    14821 tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca ggtttacact
    14881 cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct tgtccatcaa
    14941 tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc gcagctaaag
    15001 caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat aaatcatttg
    15061 tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca ccgacaattc
    15121 ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt agggttggtt
    15181 tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg gagttctctg
    15241 aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag tgattgctga
    15301 caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa
    15361 gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc cctgctcgct
    15421 ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa atgatcatat
    15481 ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga ttcatgtagc
    15541 catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt gattcagtat
    15601 gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact agtgggtcag
    15661 gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca acaagggcta
    15721 aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag tactatctta
    15781 tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta
    15841 aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc ataaaaaaat
    15901 caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg ggggataaaa
    15961 aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg caagcattct
    16021 attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg taggaatcgc
    16081 tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg tctcggaatt
    16141 agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag aaaatcaagt
    16201 agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg cttctatcga
    16261 tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca aggtgatcgc
    16321 ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt ttgataattt
    16381 tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga aagaaggaaa
    16441 aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg cactaatcaa
    16501 ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt tggtcgtgcc
    16561 atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca
    16621 agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg atgctgtttt
    16681 gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg tggggtatgg
    16741 gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg ctggcgttaa
    16801 atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta tcttagcaaa
    16861 aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg tcaatgatac
    16921 tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata cagtatctgt
    16981 cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag aatccgatct
    17041 gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga gaggataaga
    17101 attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc tggaatccga
    17161 gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa
    17221 aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc atatggctcc
    17281 tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt
    17341 gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta tctttcaata
    17401 aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga ttgggaactg
    17461 acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga cccgaatctt
    17521 ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa agcattatca
    17581 aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga agaagaaagc
    17641 gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc catcattatt
    17701 tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt acgggatgga
    17761 cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat tcgcggaatg
    17821 gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg agaagtttat
    17881 tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat cataaacgac
    17941 cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact gatgggagcg
    18001 ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa gatatccgga
    18061 cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc cattgcaaac
    18121 ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct gatggatatt
    18181 cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt attagataaa
    18241 gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc
    18301 atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt tttatccaag
    18361 tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg gatagatgtt
    18421 ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg caaagctgtc
    18481 tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat ctatacgatg
    18541 aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga agtcttgatg
    18601 aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc ttacggataa
    18661 aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc gctacagcat
    18721 ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg gtattttctg
    18781 gcgtccattg aatataacta accttgttct gcaaaatagc catatattag tactagctgc
    18841 agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt cagtgatggc
    18901 gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac
    18961 agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt tttgggtagc
    19021 ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt ttcgtggact
    19081 gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt tccaaaaaat
    19141 gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct tgacattgat
    19201 tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg
    19261 gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg cggtatttac
    19321 agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt atccgattat
    19381 tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata aaacagtagc
    19441 aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa
    19501 aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag ctttagctgg
    19561 attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat cgatggaact
    19621 ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca ttggaacaat
    19681 catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca tgtcgatttt
    19741 aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt tagccgttgt
    19801 gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt attcagaaaa
    19861 aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac attcattgtt
    19921 tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa ttctcttttt
    19981 gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa aagccttgat
    20041 taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat gtaatgaaag
    20101 tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat agccgttctg
    20161 aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta gtgcctgcca
    20221 acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat ggttatttct
    20281 atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc
    20341 gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc tttatagaga
    20401 agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg catggacatg
    20461 ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa acttttgtga
    20521 ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg
    20581 caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag acaggctttg
    20641 ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct cgcaagccga
    20701 tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc cgttttggtg
    20761 cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa
    20821 aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa ttccaaaaag
    20881 gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga tctttgaaag
    20941 atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct ggaggaagag
    21001 atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt tttaatggag
    21061 acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc ctgcattaag
    21121 taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg gaattattat
    21181 cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata agatttttgt
    21241 cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc
    21301 aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca
    21361 aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa acggaaggat
    21421 caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg gtgtgaatcg
    21481 ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa cgagcaaacg
    21541 tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc caagaatcga
    21601 ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc aaaaaataag
    21661 agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg agatgccaga
    21721 tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct tagcggaaga
    21781 aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac ggtatttgga
    21841 aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg gcggtcagca
    21901 agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag ctgtagagtc
    21961 accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt tatttgggat
    22021 accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa
    22081 attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag tgatgagtca
    22141 aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa ttattgaaga
    22201 cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta tacgaacgat
    22261 tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa aaacaacgaa
    22321 aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat
    22381 gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat ttaatgaagc
    22441 aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag
    22501 cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg gttactatgc
    22561 ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag tagtagatat
    22621 gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa tcagaatcaa
    22681 ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga aaattctaga
    22741 aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa cagcttggat
    22801 ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag aaactaagaa
    22861 aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa
    22921 ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat tagaaaatag
    22981 aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg attttattca ctaatgctgt
    23041 taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt
    23101 tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac agttttattc
    23161 actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat gtgtgacaaa
    23221 tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc aatgcagatt
    23281 tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat ttagtacctg
    23341 atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct cgtgtattag
    23401 accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca ccaactgctg
    23461 gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat gttcttcttc
    23521 caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct caagcttttt
    23581 tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa gctctaccac
    23641 cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta ggtttgaaga
    23701 aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca tcacaaggtg
    23761 atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta ttttatatcc
    23821 aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca aaaacacttg
    23881 cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg gatccgcttg
    23941 cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat ggaccagtcc
    24001 taatcgaaac actgacttat cgttatggac cacatacact atctggtgac gatccaaccc
    24061 gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg atccgcttcc
    24121 gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag
    24181 caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca aaacaaaagg
    24241 tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa gaacaaattg
    24301 caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga tccaagcaat
    24361 cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga
    24421 tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa agttcggaga
    24481 agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga gtttcggtct
    24541 tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta ttttcgaagc
    24601 aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg gcactagaaa
    24661 tctacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag aattgcattc
    24721 ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga ttccttccaa
    24781 cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc cggttgtttt
    24841 cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg aagcttacga
    24901 agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta ttatcacata
    24961 cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag aaaatatatc
    25021 tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga tcattcaatc
    25081 cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag cgggcgttgc
    25141 cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag ccccaatcgg
    25201 acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg tctggttgcc
    25261 aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg
    25321 acgaaacaaa gtcatggctt atcaatttaa attgccggat atcggtgaag gaatcgcaga
    25381 aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag atgatacatt
    25441 actagaagtt caaaatgaca aatcagtaga agaaatcccg tcacctgtta caggaacggt
    25501 taaaaatgtg attgttccag aaggtaccgt agcaaatgtt ggggatgtat tagtagaaat
    25561 cgatgcacca ggacacgaag acaatgaagg ggacagtggt gtagccgcag aatcccaaac
    25621 tccagctaaa ccggctgctg aacccactgt tgatacagaa tcagctggat cgtcaagcga
    25681 aggagtcttc caatttaagt taccggatat cggtgaagga atcgcagaag gtgaaatcgt
    25741 caaatggttc gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca
    25801 aaatgacaaa tcagtagaag aaattccgtc acctgtcaca gggacagtta aaaacgtgat
    25861 tgttccagaa ggcactgtag caaatgttgg ggatgtatta gtagaaatcg atgctcctgg
    25921 acacaatagt gcaccgtcaa ctagtgcgcc atcagctgaa gcaccaaaag aaaaagtgga
    25981 aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca aataaacgtg tattggctat
    26041 gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat atctcacaag taactgcaac
    26101 tggaaaaggt ggcagagtaa caaaagagga catcgaaaac ttcttggcag gtggtccaag
    26161 cagtgcacca gcgaaatcag aagcgccaga agcggcagca ccgaaagaag cagctcctgc
    26221 agcagaaagc aaaccagctg caccagcaaa accattcaag tccaacttag gagacttgga
    26281 agaacgtgta gcaatgacgc caacacgtaa agctatcgct aaagcaatgg tcaacagtaa
    26341 gcatacagct ccacacgtga cattgcatga tgaagtagaa gtgtctaaac tatgggataa
    26401 tcggaaacga tttaaagaag ttgctgctgc aaatggtacg aaacttactt tcttgccata
    26461 tgtcgtgaaa gctttaacag cgactgttaa gaaataccca gtattgaatg catctatcga
    26521 cgatgcaaac caagaaatcg tttataaaca ttactacaat atcggtattg caactgatac
    26581 ggatcacgga ctatatgtac caaatgtgaa agatgcagac cgcaaaggaa tgtttgctat
    26641 tgcagacgaa atcaatgaga aagcaaaatt agctcatgac ggcaaactat ctgcagaaga
    26701 tatgcgcaat ggaacaatta cgatcagcaa tatcggatct gtaggtggcg gttggtttac
    26761 accagtcatc aactatccag aagtagctat tttaggtgta ggaacgattg cacaacaacc
    26821 aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg atgaaactgt cattaagttt
    26881 cgatcaccgt atcgtagatg gagcaactgc gcaacaagca atgaataata tcaaacgtct
    26941 tttagctgat ccagagctat taatgatgga aggatgataa acaaatggta gttggagatt
    27001 ttgctgtaga attagataca gttgtaattg gtgcgggacc tggtggctat gtagctgcca
    27061 tccgcgctgc tgaaatggga caaaaagtag caatcattga aagagagtat attggcggtg
    27121 tttgtttgaa tgtcggctgt attccttcaa aagcactgat agctgcagga caccattatc
    27181 aagagtcact agattctgaa atgtttggag taactagtga aaacgtcaaa ctagattttg
    27241 caaaaacaca agaatggaaa gaaaacaaag ttgtccatac gttgacttct ggtgttggtt
    27301 tcctattaaa aaaacataag gttgaaacaa tcgaaggaga agcgttcttc gtagatgatc
    27361 acacactacg cgtcattcat ccagattcag ctcaaactta ttcgttcaat aacgcaatca
    27421 tcgcaacagg ttcccgtcca atcgagattc caggatttaa attcggagga cgtgtgttag
    27481 attctactgg cggattagca ttgaaagaag tacctaagaa atttgtgatc atcggtggcg
    27541 gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt aggagcagaa gtaacgattc
    27601 ttgaaggctc accacaaatt ttaccaactt acgaaaaaga tctagtaaaa ttagtagaag
    27661 atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc aatggcgaaa gaagcagtcg
    27721 ataacggtga cagcgtgaca gtgaaatatg cagtggacgg gaaagaagaa tccgttactg
    27781 ctgattatgt gatggtcact gtaggtcgtc gtccgaatac agatgatatg ggcttagaac
    27841 aagcgggtgt cgaagtagga gaacgaggat tgatcacggt ggataaacaa ggacgtacga
    27901 acgtacctaa catctatgcc atcggtgata tcgttccagg ggcggcatta gcacacaaag
    27961 ccagttatga agcaaaaatc gctgctgaag caatctctgg caaaaaagta gctgtagatt
    28021 ataaagcaat gccggcagta gccttcactg atcctgaatt ggcttctgta ggtatgacga
    28081 tcaaagaagc aaaagatgct ggtatcgaag caaaagcata taaattccca ttttcaggga
    28141 acggtcgggc attatcgctt ggtaaaacag aaggattcat ccgtctagtt acgacgatcg
    28201 aagataacgt gcttattggc gcgcaaatcg gtggagtcgg cgcaagtgat atggtctctg
    28261 aattagcttt agcaatcgaa tcaggaatga atgctgaaga tatcgccttg acgattcatc
    28321 ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga attagcatta ggtttaccaa
    28381 tccatatttg atttgaataa tacaggcgaa acgcaactga aaaatcagtt gcgttttgtt
    28441 ttttagaatt atcaataata ttattatgta aacttaaaaa tatgacaact tcttggttta
    28501 ttaccacttc ctatgctaca ctgaagagga atatgaaata ggagagttga agatgcaaga
    28561 atatcagtat ccacttgatt tagattggac aacagacgaa atggtcattg taatgaagat
    28621 gtgggaagat ctggaacgtg cgaatgaaaa agggataaac aatgaagctt ttttgaagac
    28681 ttatcagcag tttaaaactg ttgtcaaatc cattggtgag gaaaaacgat tgggacggga
    28741 atttgaaaaa gcttccggtt attctttata tcgtacagtg cagcaagcaa aaaaaaatcc
    28801 aaacaaaact ttgagaatga acgggtgaga taaatggaac agatgattga tcatatcaag
    28861 tcctggcttg aagaagctgg tgagaaaatc aaatcttctc aacaaacatt aacaatctcg
    28921 caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc gaaacatcca gaattttcta
    28981 atcgaaaaaa tccattcctt tgatccagag gcgaaaatat tggcagaaga agaagggtgt
    29041 aatcaattat cggatctcga tggaagagtc tttatcatcg atcctattga tggcaccttg
    29101 aattttgtga ttgaagggga aaatttttgt attatgctcg ctgtttatga atccggcgtt
    29161 ggaaagcttg gtttcattta tgacgtgatg agaaatgaac ttttttgggg tggaaaagga
    29221 ataggcgtgt ataaaaatga tgagccattg cctagaccag caaataaatc cttatcggaa
    29281 ggcttacttg gtgtgaatag ttatcttttc ggtcataata aatatggaat ccgagaaatt
    29341 gcggaaagca gcatggggac acgtatgtac ggatgcgctg gtcttgaact agtggcaatc
    29401 ttgaaaggaa accacattgc ttatatttca aaccttagcc cttgggatta tgcagcggga
    29461 aatgtcttat tggaagagtt tggtatggta acaagcggtc tttttggtac tcctttgaca
    29521 tttgaaaaaa gggaatacta tttagcaggg acttcttgtg catatgcaga aatcgcagac
    29581 cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt cacaaaaatg ataaaaacga
    29641 gcaaaagatt aattttctcg aaaaaatgaa aacattttac tatatttcat gaaaaaagtc
    29701 tgctataatt agcagtcgag taaaatttgc cctgtcataa agaaccaata aaaaaggagt
    29761 tagttacatt gaattataga aacgatatcc gtaacgtcgc aattattgcc cacgttgacc
    29821 acggaaaaac aacattagtt gatgaactat taaaacaatc agatacatta gacgcacata
    29881 cgcaattgca agaacgtgcg atggactcaa atgcgctaga aaaagaacgt ggtatcacga
    29941 ttttagccaa aaacacagct gtagactata aaggtattcg cgtaaacatc atggatactc
    30001 caggacacgc ggacttcggt ggcgaagtag aacggatcat gaaaatggtt gacggcgttg
    30061 tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac acgttttgta ttgaaaaaag
    30121 cactggaaca acatatcaca ccaatcgttg tcgtaaacaa gattgacaaa ccttctgctc
    30181 gtcctgagca tgtcgtagat gaagttcttg aactattcat cgaattagga gcagatgatg
    30241 atcagttaga ctttcctgtg atttatgcat ctgcactaaa tggtacgtca agcttgtcag
    30301 atgatccagc agatcaagaa ccaaccatgg cacctatttt tgatacaatt attgaaaaaa
    30361 tccctgctcc tgtcgataac agcgacgaac cattacaatt ccaagtttca ttacttgact
    30421 acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt tagaggaaca atcaaagtag
    30481 gagaccaagt agcattaatt aaattagacg gaacagttaa gaaattccgt gtaacgaaat
    30541 tatttggttt ctttggacta aaacgtctag agattcaaga agcaaaagct ggagacttga
    30601 ttgctgtctc aggtatggaa gatatctttg tcggtgaaac agttactcct gtggatcatc
    30661 aagatgcgtt acctatcctt cacattgacg aaccaacatt gcaaatgact ttcttagtta
    30721 ataattcacc atttgccggt cgagaaggta aattcgtaac cgctcgtaag attgaagaac
    30781 gtttgatggc agaacttcaa acagatgtat ccttacgagt tgaaccaaca aactctccag
    30841 atgcatggac agtttctggt cgtggagaat tgcatttgtc tatcttgatt gaaaacatgc
    30901 gccgtgaggg atacgaattg caagtatctc gtccagaagt tattgaaaaa gaaatcgacg
    30961 gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac accagaagaa tatatgggta
    31021 gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca agatatggtc catacaggaa
    31081 acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg tttgattggt tattcaactg
    31141 aattcctttc aatgacacgt ggttacggaa tcatgaacca tacttttgac caatatctgc
    31201 caatgcttcc tggtcaaatt ggcggacgtc accaaggggc gcttgtttcg atcgatacag
    31261 gaaaagcaac gacttattct atcatgagta tcgaagaacg cggtacagtc ttcgtagaac
    31321 caggtacaga agtatatgaa ggaatgatta tcggggaaaa cagccgtgat aatgatttga
    31381 ctgtcaacat cacgaaagca aaacaaatga caaacgtacg gtctgctact aaagaccaaa
    31441 catctgtcat caaaaaacca aaacaattga cgttggaaga atctttggaa ttcttgaatg
    31501 atgatgaata ttgcgaagta acaccagaaa gcattcgctt gagaaaacaa attttagaga
    31561 aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc tgtaaaagga tgaagagaga
    31621 aaccagcttg ttataaaaaa tagctggttt ctttttttgt ccaactcttt tctgtggaga
    31681 agtcatcttt gtcaatggac ataaattcag aaaatacgcc gaaagacgtt taaaagactt
    31741 ttaaaaaact tatttctaga gagtttttgt ttcataataa gataatgttt cacgaaagga
    31801 gatcaacttg tccttcgaag aaaatcgaag taaaatggca agagaaagga gcgggaagat
    31861 gaacgactta ttaaaacaat actttggcta tgatgagttt agacctggtc aaaaagaaat
    31921 tatccagaaa gtcattgatc aggaaaatgt attaggaatc atgcctaccg gaagtgggaa
    31981 atcaatctgt taccagcttc cagctttact attagatggg cttactgtgg ttgtgtcccc
    32041 tttaatctct ttgatgaagg atcaggtgga cgcggcgaat caattaggta tacccgccac
    32101 ttttatcaat agctcgttag atggttacga aacagctaga cgcttccaag aaatcgatag
    32161 acagcagtac cgtttacttt atatcgctcc tgaacgtttt atcatgccgg attttatcca
    32221 agcaatgaag cgatggaacg tccgtatgat cgctattgac gaggcacatt gtatctctca
    32281 gtgggggcat gatttccgcc caagttattt acagatggcg aaccagctag atcaactgcc
    32341 aaatcgtcct gtaattgttg ctttgacagc tacagcgact gtgcaagtag ctgctgacat
    32401 aaaaagactt ttaaaaattc cagaaaacaa ccatattcag actggttttg aacgagaaaa
    32461 tctacgattt caagtaatta aagatcaaaa aaaagaacaa tatctaatcg aatatttaaa
    32521 gatcaacaaa aatcaatctg gtatcattta tgcagcaacg agaaaagaag tagaccgtct
    32581 ctatcatttg cttagaaaat tcgatttttc cgtgggtaga tatcatggtg gtctaaatga
    32641 gaatgagcga acagaaatgc aagaagcctt tttatacgat cgcttgcagc tgattgtagc
    32701 gacaaatgca tttggtatgg gtataaataa aagcaatgtc cgttttgtta tccattatca
    32761 aattcctggc tctctagaag catactatca ggaagctggt cgggcgggaa gagatggtct
    32821 ttccagtgaa gccatcctgc ttttctctcc tcaagatatc caagtccaaa agttttttat
    32881 ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa tatgaaaaat taaaagcgat
    32941 gacagaatac gtgtatattg agtcctgtct tcagcaatac atcctaaact attttggcga
    33001 gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat gacagagaaa tagtagaagt
    33061 aacgacagaa gcacagatgg ttctttcttg cttaaaacgt atgggtgaga actatggtaa
    33121 acaaatgctg atgaaagtct tagcaggatc gaaagaacaa aagctgcaag ctttaggttt
    33181 tggacattta tctacctatg gactgatgaa aaaccaatcg caaaaagaaa cgatgcagtt
    33241 aattgaatat ttgatatcga atggctatct tctgaccatc aatggtgagt atcctgtttt
    33301 gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa gaatctgttt atcgaaagga
    33361 acccaagaaa gtccagcagc tatcggatga agaaaccgat acactatttg aagtgctgcg
    33421 ggaactgcga acggatctag catcagaagc aggcgttccc ccgtatgtcg tattttcaga
    33481 ttccacattg aaagaaatga gccgaatacg tccatcaagc agattggaga tgcttcagat
    33541 aaaaggagtt ggccaaagca agcttgataa atatggcgaa gcctttttat ctcggataaa
    33601 aaatgcggac tctaacgttc tggataagta gaaagaacga atacgtaaaa atgaataaaa
    33661 acaactggtt aaatctgttt tttttgctcc ttatgaaaat caaatatgtt ataattaagg
    33721 aattagaatg ggaggtaagc acagatggat tctttgtcga aaaaacttgc gatccaagta
    33781 ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa atcaaaacaa cagtctctgt
    33841 atctcacaat gcaaagcctt cgaagaagtg gtagatacac agatgtacgg cttttcccgg
    33901 caagtcggtt ttgctgtccg tataggagta atcggaaaga acgagggcca gctgatgtta
    33961 agtgagctag aacgagaatt gaatcagtta tatagtgagg tttaccaaga aatttatgat
    34021 aaaaaagaag ttggcaagga ggaatagact tgcctaagaa agtgaaaaaa aggcatctat
    34081 tagactatag catcttgatt ccctatttaa ttttatgtgt attgggattg atcatggtat
    34141 atagttctac gtcgtatcta ttattagaaa acggacaaaa tccgtccgca tcagtcatta
    34201 accagagtat tttttgggtg cttagcttga ttgctatcgc actactttat aaaatgaaaa
    34261 cagatgtttt gaaaaaccag cgtttgatca tggcagccat cgcagttttg acgattttgc
    34321 tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc gaaaggttgg ctgcaaattg
    34381 caggattctc gatccaacct gccgaatacc taaaaatcat ttctatctgg tacctgtctc
    34441 tgactttatc taaaagacaa aacagtgtac aaaaagattt tcttgggact gtgaaacgtc
    34501 cacttgcaat ggttatcgga cttactgcat tagtcgctat tcttcccgat tttggtaatg
    34561 cggcagttat ctttttgatt attttggttc tgcttttagc cagcggagtc aattatgtgt
    34621 atactttgat tgtaggagta ggaggctttt gtctaagtac ttttacgatc tggctgatca
    34681 atatcacaaa cggaaagatc ttacccggac gtttacagta tatctataat cgatttgcta
    34741 tttaccaaaa tcctttctca gacgaattaa ataaagggca tcaactagtc aacggctact
    34801 atgccatgtt caacggagga ctatttggca ggggattagg taacagtatc cagaaaaaag
    34861 gatttttaca agaagcacaa accgatttta tttatgcaat cgtagtggaa gaactgggag
    34921 tgattatggg gattttgata ttagcgttgc tattctttat gattgtgcgc attgtccttg
    34981 taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg cattgggatc ggcgcaatgt
    35041 ttttgatcca agtatttgtc aatcttggag ggattactgg cgttattcct ttgacaggga
    35101 tcacgttccc atttttaagt caaggaggct ccagtttgct gatgctttcg atttgtgtcg
    35161 gctttgtcct caacatcagt gcggatgaaa aacgcaagag cttaggttta taataaaaaa
    35221 agatgtagga gtggttcgat gaaaaaagtt ttagttgcta acaggggtga aatcgctgta
    35281 cgtgtatttc gtgcttgtac agaattagga attaaaactg ttggaatcta tgcagaagaa
    35341 gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat atttagtagg acaaggaaaa
    35401 aaaccgattg atgcgtatct agatattgaa gggatcattt ctatcgcgaa agaatgcgga
    35461 gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa acttgaattt tgcgcaacgg
    35521 tgcgaagaag aaggcatcat ttttgttgga cccaaactgc atcatttaga tatttttgga
    35581 gataaaatca aagcaaaagc tgcagctatc gaagcgggga ttgcttcgat tccaggcaca
    35641 gatggaccaa tcgcttctat agacgatgcc ttggagtttg cgaaacaata cggttatccg
    35701 attatgatca aggcagctct aggcggcggc ggacgcggca tgcgtgtagc tcacgatgaa
    35761 aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag caaaagcagc ctttggtagt
    35821 gatgaagtgt atgttgaaaa atacatcgct aatcctaagc atatcgaagt acagattctc
    35881 ggggatacgc atggaaacgt catccatctt tttgaaagag attgttctgt tcagcgccgt
    35941 caccaaaaag tagtagaagt agcgccttgt gtctctatga atgaacagca acgtcaaaaa
    36001 atctgccagg cagctgtcca attgatgaaa tatgtcgggt atgtcaacgc aggtacagta
    36061 gaatttcttg ttgaaggaga tgatttttac tttatcgaag tcaaccctcg cgtccaagtg
    36121 gaacatacga tcacagaaat gattacagat attgatatcg taacaacgca actgttgatt
    36181 gctcaaggtt tggatctgca taaagaaatc ggattgcctc agcaagaagg aattaaatta
    36241 aatggttcag ccattcaatg ccgaatcact acagaagacc cgttgaataa ctttttacca
    36301 gacacaggga aaatcgatac gtatcgttca cctggtggtt ttggcgtacg tctggatgtc
    36361 ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg attcattgct agtaaaagta
    36421 tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa tggaacgttg cttgaaagaa
    36481 tttagaatcc gtggagtgaa aacaaacatt ccatttatgc tcaatgtcat cacacatcct
    36541 gaattccaat caggaaatgc aaaaacaacc ttcattgata gtacagcaac attatttgaa
    36601 tttcctcgac tacgagaccg cggaaacaaa acgatgaaat atatcggtga aattactgtg
    36661 aatggttttc caggtatcga aagtggcgaa aaaccatttt atgaagaacc acgtatgcct
    36721 aaagatctaa tcacaagaac tgattacgta acggctaaga atgtattaga tgcgaagggt
    36781 gccgatgctt tggtcgaatg gatcaaagga caggaaaatt tgttgttgac ggatacgaca
    36841 tttagagacg cccatcaaag tctgctagca actcgtgttc ggacaaagga tttcaagcag
    36901 attgcacgtt tgactggaga aggtcttcct gaactctttt caagtgagat gtggggggga
    36961 gcaacatttg atgtggctta ccgctttttg aatgaagatc cttggcaacg gttgagaaaa
    37021 attcgttctt taatgcctaa tacgttgctg caaatgcttt tcagaggttc aaatgccgtt
    37081 gggtattcta attatcctga caatgtattg gtggaatttg tcaaagaagc agcagcgcaa
    37141 ggaatcgatg tatttcgaat ctttgatagt ttgaactgga ctccacaaat ggaaaaaagt
    37201 atccaagctg tacgggatac tggtaaaatc gcagaggcag ctatttgcta tactggcgat
    37261 atcaacgatc caagtcgggc aaaatacaat gttcagtatt ataaagacat ggcaaaggaa
    37321 ctagaacaac tgggtgcgca tatcattgca atcaaagata tggctgggct gcttaaaccg
    37381 caggcagctt atcgtttgat tagtgaattg aaagaaacga cagatctgcc aatccatctg
    37441 catactcatg ataccagcgg gaatggaatc atcacttatt ctgctgcttc aaaagcagga
    37501 gtagacatcg ttgacgtggc aatgagtgca atgagtggga atactagtca gcctagcatg
    37561 agcagcttgt attatgcact ggtcaatggt ccacggctgc cagaaatcac gattgaaaat
    37621 gcacagaaat taaaccatta ttgggaagat gtgcgcatgt attacaaacc gtttgaaaac
    37681 ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa tgccaggagg acagtattcg
    37741 aatctacagc aacaggcaaa agctgtcgga ttaggtcatc gctgggatga aatcaaacaa
    37801 atgtatcata ctgtcaactt gatgtttggc gatatcgtta aagtaactcc atcatctaaa
    37861 gtagttggag atatggcact atttatggtc caaaatgatc ttacagaaga agatatttat
    37921 gaaaaaggcg aaacactaag tttccctgaa tctgttgtga ctttcttcca aggagaacta
    37981 gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga ttattcttaa aggcagacct
    38041 gcattgaatg aacgtccagg tcttttggct gaatcagtcg acttcaacga agtaaaaaaa
    38101 gaattggcag aaaaaattgg ctatgaacca aaacaagacg aagtattaag ctatttgatg
    38161 tatccgcaag tctttttgga ctatcaaaaa gcatataatc aatttggcga tgttacatta
    38221 ctggacacac caacattttt ccaaggtata cgtttgggtg aaacaatcaa tgtacaaatc
    38281 gaacgcggaa aaacgcttat cattcgttta gatgaaatcg gagaacccga tatcgaaggg
    38341 aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg aaatcactat caaagataat
    38401 tcgatcatca gtacggtcca aacccgattg aaagctgaac cgacaaaccg agaacaaatt
    38461 ggagcaacga tgtctggttc tgttttggat gtattagtga aaaaaggcga taacgttaaa
    38521 aaaggtgata cgttgatgat tactgaagcg atgaaaatgg aaacggccat cgaagcacgt
    38581 tttgacggag aagtcgcaca tgtatatgtg tcttcaggag acactatttc gtctggtgac
    38641 cttttgattg aagtgactga gaaataataa gcgaaaggtg gggaaacatg aaaagactgg
    38701 gtttgttttg gctgattctg ctattagtta ttgtggcatt ctacatggaa ccagtcttgt
    38761 ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca aatatcaaac caaatgacaa
    38821 cgtggaaata ccaggagctt aaagcaactg gtttttctac ttatatcgga aaaccgatcg
    38881 atcagctgga aaaggatttt ggccaagcct atgatcgatt aactagcgga tttggttttg
    38941 aaacaagata ttatgaaaat aaagctagtc atctcacatt tgaagcgaac atccaagaca
    39001 gcagagtcac aactgttaaa gtactgcaaa cagggcctgc tgatatcgcg ccgttttatt
    39061 tagggatgac gatgcaagat cttacgaaaa tcactactat ctatactaat ttcacttttg
    39121 agtaccatga tacagaagtt ggaatcgaac taatggaaga ggatatgaat tatcgtccgc
    39181 taattgcttt tgataacgac acttttgcta ttttattttt tgatcaaaca acaaacggcc
    39241 tattttcagt tgtgtattta gataaagata gtcttttgaa actattgcca tatcaagtgt
    39301 ttggtgaagg ccttccacat tatcaagcag ataaatcggc tgattgggaa tcaatcaatc
    39361 gcgttaaaga acgaaaaagt acgactttat taaatatgct tagaagagaa gacgaacttc
    39421 ccatatacaa tcaaacactt gcctttcaaa accacagtca gctgctacta catgattttt
    39481 tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc tgattggcaa aaatcgctag
    39541 gcagtgctca aacgacagtg aaattctcac tgacgaatga tgaattggag aagttaatca
    39601 gtaaacaaaa attagaaaat actagtgggc tatttattca tccggcgatt gacgctactt
    39661 tttcgttttt atattggtac agcgatcctt acaaccatga ccgtttcatg gacccgggaa
    39721 cggattctgt aggcattgct ttttctaaag aaaatatgtt agtcttattg caagaagaaa
    39781 acggagaaag tagtgatagc agtgatcgtt aacgaagagc tgtttcaact ggaggatcaa
    39841 tgcctccaag tcgctgaaat gataaaaaag agtaaaacga tggcttctta tcttgaacat
    39901 aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga aagaagcgtt cttgtctaag
    39961 aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg cacctgatta tcgtgaaaaa
    40021 aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg atgagcatgt tgcagctttc
    40081 cgatatagtg aaacacagct gcaaagtctg ttggatcaaa ttgtccaagt acttgccgaa
    40141 gaagtttcta atgaaatcaa agtagatgca ggtagcccgt tttttgaaac gaaaaaacac
    40201 tttggatgcg gaggtaattg tcatgcaatt agacaatagt gaatttgaaa taacaaaacg
    40261 acgcgggctg atcgtatggg tctacagctt aaaacaatta aagaatctga aaaagttcgg
    40321 cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg ctttacttaa atgaagagac
    40381 attggaagaa acagaagaac gaatccgtaa attacatttt gttcgaaacg tagaacgttc
    40441 ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata ggaaacaaaa cagtattaaa
    40501 agacgaaggt ttcgaaattg aagagctaaa tacagaaatc cgattagctg aacatcttta
    40561 aaaatctctt atgagcagtt tttaaataaa aaataacttg gataacgatc gatttttgat
    40621 cattgtccaa gtattttttt ggtatactag taccagtacc atttctaagg agtgagtaga
    40681 tgcggataat atcaggaaat tatggcgggc gccgtttaaa aagtttagca ggggcgaaca
    40741 ccagaccaac tacagataaa gtaaaagaat ccatattcaa tatgattgga ccatattttg
    40801 atggagagac agtattggat ctgtttgcag gaagcggtgg tttagctatc gaagcagtat
    40861 ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta ccaagcgcta aaaatcatca
    40921 aagaaaacat cgaaattacg aaagaaccag agaaatttac cacaataaaa cttgatgcgg
    40981 acaaagcaat cgaacggcta gcagcggaga aacaacaatt cgactacttg tttcttgatc
    41041 cgccatatgc aaaacaaaaa ataatcgatc aaatccataa aatggaagaa ctgggacttt
    41101 tttctaaaac cgcagttatc gtatgcgaaa cggataaaac agtcgaattg ccagaaactg
    41161 tggctggatt ccatcaaata agaaaacaga tttatggcat aacggccgta actatctata
    41221 gaaaagaggg aaatcaatga aacgtgcatt attcccagga agttttgatc cttttacaaa
    41281 aggtcactta gatacggtgg aacgtgcggc taaattattc gatgaggtag taattggtgt
    41341 ttttatcaat acttcaaaga aaagtttgtt tcccccagaa gaaagaatga cattgatcac
    41401 aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat caagaaaatc aattgacggt
    41461 agaaacagca aaagagattg gtgcagatgc gttgatcaga ggcatacgaa gtatcaaaga
    41521 ctttgaatat gaacgagaga ttgctcagat gaatcatcat ctatatgatg aattggaaac
    41581 tgtcttctta ttagcaaaac cagaatatag tcacgtgagt tccagtattt taaaagaagt
    41641 actgcacttt ggcggagatg tgagcagcta cttaccgcca gtgatcaacg aagctttagc
    41701 tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa aacagtactg attatcatat
    41761 ttggaatgtt gatcattgga tttgtagtag ttcccttgcc gtattatgta gaatcaccgg
    41821 gagccacgat cgatctgaaa gaactaatta cggtcaatga taaggaagat aaaggttctg
    41881 ggtctttctc tcttacctct gttggtatca ggcaagctac aggtttctta gctttaaaag
    41941 ctaaaatgac cccttttgaa gaattgatca gtgaagaaga attgactggt ggggcgacaa
    42001 atgaagaata taatcaaatg caacagtatt atatggattc ttcccaaaat gcagcaatcg
    42061 aacaagcgtt aaaacttgca gacaagcctt ataaaatgga attcaaaggc gtatatgtca
    42121 tgagtgtaga gaaaacatcg aatttctttg gcaagctttc agttggagat acagtaacaa
    42181 aagtagacgg gaaaagcttt caaagtacag aagcatttat ggattatgta aaaagccaaa
    42241 aagttggaca aacaatcgaa atcagttatt tgcataatgg tgaagaaaaa acagcttctg
    42301 gtgatctgat cgagttacca acagataaaa aggcaggaat aggtatcacg cttactgacc
    42361 ataccgagat cgaatcagat acagatgttc gtattaattc tggctctatc ggtgggccat
    42421 cagcgggatt gatgttcact ttagaaatct atgaacaagt cactggtaaa aacttgcgac
    42481 atggtaagca aattgcagga actggaacga tcaacagtga aggagaagtt ggccgaatcg
    42541 gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg cgtagagatt ttctttgcac
    42601 cagatgatac gatttcagac gatgttaaga aagaatatcc tgaaataaaa accaactacg
    42661 aagaagcaaa agctgccgct aaaaaattga attcctcaat gaaaatcgta ccagtaaaaa
    42721 cagtacaaga agcgcttgac tatctagaga aaatgaaatg aaactaaata attaaggctc
    42781 ttcgtcaaat tgtgttggcg aagacaaatt tgaataaaac ttagtccgat atgcggcgcg
    42841 ttctgtgcgc cgcatatttt tttagttttc aaagatcaga ccttgttgta aatagatccg
    42901 tgcccgaaag ttataaaagt tgcgataacc aaacgcaatt cgtttaatca gcttgatctt
    42961 gttattcagc ccctcagtga cgccgtttga gtggggcacc ttaaaggcat ttgaaatacc
    43021 cgattggtag cgatggaaga tcgtgaattt ctttttaaac gactgaggta acgtagatga
    43081 aattgtttta agggtttctt gaaagccttc cgtatctcgt ttaacgaaat aatagcgtaa
    43141 taattgacaa aaatgatacg cagttcgcaa ttcttcgttg tagcttaaca gctcatcaac
    43201 gatttctgtt tgactaattg gtcgtttaaa cagtcgatga taatgccgtt tgagtggctc
    43261 caatgtcgtg gaatctttga gtaagagttt ccaatatcgc ttgagccgac ggtacttctt
    43321 ctgattttca gagtgtgagg ttcgaaaacg attcatgatc tggacacgta attgattgaa
    43381 gctgcgattg atgtgttgga caatatgaaa gcggtcagtt acgatctccg catttggaaa
    43441 gactgtttta agcaactgac aatagctggc attcatgtcc atcactaggt acttgacctt
    43501 taaacgggcc tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc
    43561 ttcaaggatc gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc
    43621 agaaacagac ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat
    43681 acgtggctgg taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac
    43741 gaagtacttc ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc
    43801 ctgcttcagt tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa
    43861 ggtccgtcca caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt
//
LOCUS       DACDHL010000015        43713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDHL010000015 DACDHL010000000
VERSION     DACDHL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.015"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(353..2734)
                     /locus_tag="IV228_000858"
     CDS             complement(353..2734)
                     /locus_tag="IV228_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8716795.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2724..3371)
                     /gene="recU"
                     /locus_tag="IV228_000859"
     CDS             complement(2724..3371)
                     /gene="recU"
                     /locus_tag="IV228_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8716796.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3435..3974
                     /locus_tag="IV228_000860"
     CDS             3435..3974
                     /locus_tag="IV228_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8716797.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4064..4483
                     /gene="gpsB"
                     /locus_tag="IV228_000861"
     CDS             4064..4483
                     /gene="gpsB"
                     /locus_tag="IV228_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8716798.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4505..4872
                     /gene="rnpB"
                     /locus_tag="IV228_000862"
     ncRNA           4505..4872
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV228_000862"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5081..6247
                     /locus_tag="IV228_000863"
     CDS             5081..6247
                     /locus_tag="IV228_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8716799.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6293..7789
                     /locus_tag="IV228_000864"
     CDS             6293..7789
                     /locus_tag="IV228_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8716800.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7870..8553
                     /locus_tag="IV228_000865"
     CDS             7870..8553
                     /locus_tag="IV228_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8716801.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9198..10388
                     /locus_tag="IV228_000866"
     CDS             9198..10388
                     /locus_tag="IV228_000866"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8716802.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10490..11443
                     /locus_tag="IV228_000867"
     CDS             10490..11443
                     /locus_tag="IV228_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8716803.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11512..12861
                     /locus_tag="IV228_000868"
     CDS             11512..12861
                     /locus_tag="IV228_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8716804.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13044..13367)
                     /locus_tag="IV228_000869"
     CDS             complement(13044..13367)
                     /locus_tag="IV228_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716805.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13496..14659
                     /locus_tag="IV228_000870"
     CDS             13496..14659
                     /locus_tag="IV228_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716806.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14729..16078
                     /locus_tag="IV228_000871"
     CDS             14729..16078
                     /locus_tag="IV228_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8716807.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16123..16554)
                     /locus_tag="IV228_000872"
     CDS             complement(16123..16554)
                     /locus_tag="IV228_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716808.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16942..19584
                     /gene="alaS"
                     /locus_tag="IV228_000873"
     CDS             16942..19584
                     /gene="alaS"
                     /locus_tag="IV228_000873"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8716809.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19784..20380
                     /locus_tag="IV228_000874"
     CDS             19784..20380
                     /locus_tag="IV228_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8716810.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20502..21650
                     /locus_tag="IV228_000875"
     CDS             20502..21650
                     /locus_tag="IV228_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8716811.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21688..22101)
                     /locus_tag="IV228_000876"
     CDS             complement(21688..22101)
                     /locus_tag="IV228_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8716812.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22180..22611
                     /locus_tag="IV228_000877"
     CDS             22180..22611
                     /locus_tag="IV228_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8716813.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22710..22943)
                     /locus_tag="IV228_000878"
     CDS             complement(22710..22943)
                     /locus_tag="IV228_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8716814.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23126..24796
                     /locus_tag="IV228_000879"
     CDS             23126..24796
                     /locus_tag="IV228_000879"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8716815.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24943..25329
                     /locus_tag="IV228_000880"
     CDS             24943..25329
                     /locus_tag="IV228_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716816.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25316..26014
                     /locus_tag="IV228_000881"
     CDS             25316..26014
                     /locus_tag="IV228_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8716817.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26019..26483
                     /locus_tag="IV228_000882"
     CDS             26019..26483
                     /locus_tag="IV228_000882"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8716818.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26480..27391
                     /locus_tag="IV228_000883"
     CDS             26480..27391
                     /locus_tag="IV228_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8716819.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27626..28165
                     /gene="pyrR"
                     /locus_tag="IV228_000884"
     CDS             27626..28165
                     /gene="pyrR"
                     /locus_tag="IV228_000884"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8716820.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28179..29462
                     /locus_tag="IV228_000885"
     CDS             28179..29462
                     /locus_tag="IV228_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8716821.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29475..30401
                     /locus_tag="IV228_000886"
     CDS             29475..30401
                     /locus_tag="IV228_000886"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8716822.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30420..31700
                     /locus_tag="IV228_000887"
     CDS             30420..31700
                     /locus_tag="IV228_000887"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8716823.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31705..32781
                     /gene="carA"
                     /locus_tag="IV228_000888"
     CDS             31705..32781
                     /gene="carA"
                     /locus_tag="IV228_000888"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8716824.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32786..35971
                     /gene="carB"
                     /locus_tag="IV228_000889"
     CDS             32786..35971
                     /gene="carB"
                     /locus_tag="IV228_000889"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8716825.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36025..36801
                     /locus_tag="IV228_000890"
     CDS             36025..36801
                     /locus_tag="IV228_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8716826.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36798..37730
                     /locus_tag="IV228_000891"
     CDS             36798..37730
                     /locus_tag="IV228_000891"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8716827.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37727..38443
                     /gene="pyrF"
                     /locus_tag="IV228_000892"
     CDS             37727..38443
                     /gene="pyrF"
                     /locus_tag="IV228_000892"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8716828.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38450..39079
                     /locus_tag="IV228_000893"
     CDS             38450..39079
                     /locus_tag="IV228_000893"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8716829.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39067..39381
                     /locus_tag="IV228_000894"
     CDS             39067..39381
                     /locus_tag="IV228_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716830.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39419..40060
                     /locus_tag="IV228_000895"
     CDS             39419..40060
                     /locus_tag="IV228_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8716831.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40091..40795
                     /locus_tag="IV228_000896"
     CDS             40091..40795
                     /locus_tag="IV228_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8716832.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40792..41694
                     /locus_tag="IV228_000897"
     CDS             40792..41694
                     /locus_tag="IV228_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8716833.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41852..43558)
                     /locus_tag="IV228_000898"
     CDS             complement(41852..43558)
                     /locus_tag="IV228_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8716834.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14010 a         7702 c         9489 g        12512 t
ORIGIN      
        1 catactatca gataactcaa aatgaagcct tctttcaaaa aattattcaa aaccatatat
       61 tgtatttttt atttgataat aacaatggga ttattaagaa gtggcactaa ataaaagact
      121 agtgtgtatt tagccgattt aattataaac aagtaaatgg ataaagtata tgtaatgata
      181 aaagaaaaat ggattttcta atttttaagc taaatgttag atcacacatg tatatgctgg
      241 atagtcttat aaaaaatcat gtaatacagc ttggtacatg atttttattt gtagctgttt
      301 tttacataga cacaaaaaag aaaccacgct tgtagacgtg gtttcttttt tactatctgt
      361 ttccagaagc tgaggtgctg ctggatggtg tatggtcatt tgcagcatcc gctccgctag
      421 ttgctggctc actggaagaa gcaggaggag cagatgaaga cggtgtcgag ctttcttcgg
      481 atgaagaaga aggaaccgtt gttgaagacg ctggctcgct tgtttctgct gtcgtgcttt
      541 cttttgagga ctcagcagtt gattcgcttt gactagtaga gctagagctt tgagtcgttg
      601 tctctgttgt tgagcttcca ggagtctgga catagctgga agatggaata gttgttgatg
      661 gtgtaattgc attcgatcta gctgtatatt gatctttgta gtacagttca ccgcccacac
      721 gaattaatcc gcttggcatc tcccagtctg tatttgtcac gttagctgaa acatactgca
      781 taagctcgcg ataaacatca gaggctacgt gtgacgattc tgaagtgaca ggtgtcattt
      841 ttttattgta acctgtccac acggaaatcg aataatttgg cgtatagccc gcaaatagga
      901 tatctggata caccccagaa gaaataccaa gttttgcata ctcgtcatct gtgtaattgg
      961 atgttccggt cttaccggct tgatataacc cagcgatttg cgcattcgta ccagttcctt
     1021 cagtgatcgt atcttttaaa atgtctgtga tcatataagc tgtttctgga gacatggctg
     1081 tttttccatc tggctcataa gtttcttctg ttccgtcttg gaagacgatt ttattaacat
     1141 attgaggttt gtaataagtc cctccattgg cgaaagccgc ataggctgct gccattttta
     1201 aggaagaagc tccgtatttt gtcccatctt gttcttccgt attactagag atcgcattcg
     1261 attgatggat cgtactgtat tcgatcccaa gattttttaa gaaagaagcc actttatctg
     1321 aaccgacttc attgaataat ttgactgctg gaacattacg ggataagtaa agtgcttggc
     1381 gtaaagtaat ggtgcccata tattgattat cccagtttcc tacaggagtc gaagtccctt
     1441 catagttata cggtgcgtca gtaatagttt ttccagtaga atatttcaaa tattcaaaag
     1501 ctggtccgta atctgtgaca ggtttcatcg tagaaccaaa atcacgggaa gtattcactg
     1561 ctaagttgtt gcctaaagtt acgtcttctg cgatgtgacg tccgccaatc tgggctttta
     1621 ctttcccggt attggtgtcg ataagggtgg aggcaacttg catctcgtcg tctgggtaag
     1681 agacatactg atccgtgttg acgatatcat atagtttctt ttgggcgtcc agatccaaat
     1741 tcgtataaat ctctaaaccg tctgtgtaga cattcttatc tgttttttct tgtacttcat
     1801 tgatgacttc tttgacataa ttatcgacga tcttagtatt gtcatcggat tgagttagtt
     1861 cttgcagacc atccgttacc ggtacattaa cagcttgatc atattctgtt tgagagattt
     1921 tttcgttttg aagcatcgta tacaaaacag tatctcgtct tttcttcgcc tgatctggat
     1981 aaacataagg atcataggcg gaaggggctt gaggcatccc agcaagcaat gctgtttgcg
     2041 gtaaagaaag ttctgataat ttcttaccaa agtacatctc agaagctgtc tccatcccgt
     2101 acaagccgtt tgacatatac actttattta cgtagtaggt caagatctct tgtttcgatt
     2161 ttttttgttc caatcttaca gccatccacg cttcttgggc cttgcgtttc agagtctgat
     2221 cctcggcact ggtggagaag aaggaaagtt tgatcaattg ctgtgtcaaa gtacttccgc
     2281 cttgcagtcc gccagaggta aagttagaga gggcagaacc gatgatccga atagggtcaa
     2341 ccccgatgtg tttataaaaa cggcgatctt ctaccgacac gatcgcatct tctaatgttt
     2401 tcggtaattc attcgcagaa attttttcac gcttttctgc gcctaaatct tcgaatagtt
     2461 cgccatcttg tgtatatagt ttggaagata cggtagcgtc taatttttta tccgtcaatt
     2521 cgggcgcatc ttttgcgtaa taccagaaaa gtcctacacc tgctaaaaag aggatacaga
     2581 aaaatgagag tattcctaaa aggatcttga taaataatcc ttttttatgt gttccagagg
     2641 actttccaga acctttgcca gaattcttct tcacactctt tttaggagta ggctgtttct
     2701 gtcgtctaga gcttctagtt tgttcatttg ccattcttgc tttctccttc aatagataat
     2761 gttgataaat agaagtcaac cgcgtccaaa taaggaatcc gcggagaaat accagttttg
     2821 atctctattc ctagttcttc gatcaaagat aaaggtagtg atttgcgtcc tttttccttt
     2881 tgggcgtccc agtaagcaat caaatgaaca ctattcaaat aaaaacagcg cttcaaagac
     2941 gaaaaccata acaagacaaa gcagacccca ttttgttcga ggcatgcttt catatgttcg
     3001 atttgatggg catggaagtt tttaaaggga aaagatgttt tgttcttggt ttcttttgct
     3061 tcaaagtcca agtaaaagcc tcgatagacg ccattatagt ctgtagttga ggcttgggta
     3121 aagtaggctt ccttgatcac agcagcactt ctcttaggat agtctacttt cacgatttgg
     3181 ataggggtag gctttttatg gataaccgcc aacccgcggg aaagataata tttattactt
     3241 tcattgattg cttcttcaaa gcgcatcccc cgattgccga attcagtgat ctgatttctt
     3301 ttttcttgaa ttttttcgac gggagaggca ttcttataat aaggttggcc atttggatag
     3361 cgcaatacca tctttaatca cactcctagg ccacattata gcaaaaaagc ggatagaaag
     3421 aaaaggaatc tgagatggat aagtcaaaag taatctatgt gtctgggtat cgaagtttcg
     3481 aactcggtgt ttttaaagaa aatgatccta aaatccaagt tgttaaaaat gttttaaaaa
     3541 aagaactcca tcagttagca gaagaagggc tggaatgggt acttgtcagt gggaatttgg
     3601 gcgtcgaact atgggctgtg caaatagtag cggaattgaa agaagaatat ccggaactat
     3661 ctcttggtat tttgtatcct ttttctgatt ttggtaataa ctggaatgaa gggaaccaag
     3721 aaaaaaaaca attagcagaa caattagcag attatgtcga agctgttagc catcaaccct
     3781 accaatcacc tagtcagcta aagaatcaca ctcgttttat acttgagcat acagtgggct
     3841 gtctgctgat ctatgatgaa gaattccctg gaaaaacgaa gtattttttt gaagatgcac
     3901 agaattacca agcagatcat ccctacgaaa ttcggctgat cagtatggac gatcttcaaa
     3961 attttagtga ataatcgata ttgtgtttga tttatcaact tttttcctat acaatgaaaa
     4021 aggtagaaat gttattacgt aaatgaaaac tagaggtgta aatatggcga atttggttta
     4081 tagtcctaaa gacattttac aacaagaatt caaaacaaaa atgcgcggct atgatcccgt
     4141 tgaagttgat gaattcttag acaatatcat caaagattat gaaacttaca gcaaagaact
     4201 ccttgcttta caagaagaaa acgatcgatt gagtgcaaaa gtagctcaat tatctaaaac
     4261 tcaaggagca gctcaaacac gcgtgcaaca aacagaggca ccaaagagcg cagcagtgac
     4321 caattttgat atcttaaaac gcttgtcaaa tcttgaacgt gaagtatttg gtaaaaagct
     4381 tgatcaacaa gcaagtgcag tcaaaccagc acaacctaat ccaaataact acacaaatgc
     4441 agacacaagc ttagatgata atgaaaaaac acgtcaattt taatttttga ttaatgaatt
     4501 tgcggttttc gggtaatcgc ggtctgactt gatcaggctg aggaaagtcc atgctcgcac
     4561 aggctgtgat gcctgtagtg ttcgtgctta gcgaaaccat aagctaaggt actctttgga
     4621 gtaacggcag gaaaacaagc taaggcttcg gctatgctta agtatccttg aaagtgccac
     4681 agtgacgaag cggctgggga aacccatccg gtggaacgcg gtaaacccct cgagcgagca
     4741 acccaaacaa tggtaggggc gctttttgtc tggaagaaga acaggacaaa aaggcagtat
     4801 aactgcagat agatggttac cgctttgttt tttctccctg gaaaagcaaa gtacagaaca
     4861 tggcttatag aaaaccgcaa acatcaggga attaggtcgt gacaaaagta atgcgttata
     4921 agaaacaaga aaaacatttg aaagctattt ttcaatactt cagatgaatc gttcttatta
     4981 caaaacaaac ttttgtatca ccggttattc ggaatttgag taaaagtgtg gggtaggact
     5041 gatgtcccac ctcctttttt tataaataag gagattgtgt atggaaaaaa acaggaagtt
     5101 caacctcgtc gcaactgcgg caagcgggct agaagcactg gttggtaaag aattaagaga
     5161 tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt tttgaaggaa caatggaaac
     5221 gatcgctaca gctaatttat ggttacgtac agcagatcga gtaaaaatcg tggttgggga
     5281 attcgatgcg tatacgtttg atgaattgtt tgaaaacgta aaagcattgc cttgggaaga
     5341 ttttcttcca ctagatgcag cttttcctgt agctggaaaa tcgatcaagt cgaaactata
     5401 tagcacacct gattgccaag cgatcacaaa aaaagcaatc gttgaacgtc tccgtaccta
     5461 ttatcaccgt ccagcttctg taccactgac ggaaactggc gcacattttc agttggaagt
     5521 agctttgctg aaagaccatg tgatggtaac tctggatacg acaggaccaa gtttattcaa
     5581 acgtggttat cgcttagaaa aaggtggagc accattgaaa gaaaacatgg cagcagcact
     5641 tattatgttg acaaattggc gaaaagaccg tccattttat gatcctgtat gtggttcagg
     5701 tacgctttgt attgaagcag ccttaatagg ccataatatc gcaccaggat tcaatcgtga
     5761 gtttgcttgc gaatcttggg attggttcag tcaagaaatc atggaaaatg ttcgatcagc
     5821 agccgaagaa aaagcagatt atgatattga attagatatt actggttcag atatcaatgg
     5881 acggatgatt gaaattgcca aagccaatgc agaagaaatc ggattaggct cttcgattac
     5941 ctttaagcaa caagcagtca aagacttcaa aacggataaa gaatacgggg tcatcgtggc
     6001 taatccgcct tacggagaac gtctaggaga agaagaaact gttcggaaat tatataaaga
     6061 aatgggcgat gtattccgtc cattaaaaac atggagtaaa tatatcttga ctagcgactt
     6121 agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa agaaaattgt acaatggtgc
     6181 attgcgtacg gatctattcc agtattgggg aactcgtccg cctagaaaaa aagagaaaga
     6241 cgaataatct tactaattga ttcattcaat tggatgtttg gaggaatagg gaatgaaaga
     6301 aaaagagttt ttacaagaag taaaagaaat caatcttcta caacaagctt taggaatcct
     6361 cgattgggat acacaaacag gtatgcctga aaaagctagt gcataccgaa gtgaagtaga
     6421 cagctatctt tacagcattt atttttcaaa aaaaatcggt ccaaaaatca aagaagccat
     6481 tcgctatttt tctgaacatc cggaagaatt gtcggaagtt ggaactgccg tttttgccaa
     6541 agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa gatcttatga gaaaatatac
     6601 agctgccgtt tcttcagcac acggacaatg gcaaaaagct agagaaacac aacaattttc
     6661 tgattttcaa cacgcattga atcaaaatat tgaattaaca aaacagttga taccttactg
     6721 gagaaaagat gaaaagacga attatgatgt tttgctgaat caatacgaac ccgggatgac
     6781 tgtcgaaatc cttgataagg tatttgatca gcttaaagaa ggaattcttt cgatccgccg
     6841 tacgctccaa gaaaaaggga ctgaacctga aacagacttt ttaaatcgga gaatgacgaa
     6901 aaaacaacag aagagatttg tagtgaaagt aatcgaacaa cttggttatg attttagtcg
     6961 tggtcgttta gatgacactg tccacccatt tatgactgga attaatccga atgatgcacg
     7021 aatcacgaca cgatggaacg aaacagattt caaaatggct gtttttggag tgatccacga
     7081 ggctggtcat ggtatgtatg aacaagacat cgatgataaa tatgcgtata caccggttta
     7141 tgaaggtaca tcaatgggta tccatgaatc acaatcgttg tttaacgaga tcattattgg
     7201 aagtagtcgc agcttctgga aaaagcaata tcctttcttt caagaatgtg cagaaggaac
     7261 attcgatgat attccttttg acaaatttta tcgtttattg aaccatacaa aatcaagttt
     7321 ggttcgtatc gaggcagaca gtttgactta ccctctccat atcatcattc gttatgaaat
     7381 cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa ctacctgaaa tatggaatca
     7441 aaaatatgaa gagtatctgg gaatacgacc aacaaatgat gtggaaggca tcctacaaga
     7501 cgttcattgg tcaggtgcaa gctttggtta ttttccctct tatgcattag ggtacatgta
     7561 tgcggcacaa ttacttcatt ctttgcagca agaaatagat gtggaaaatg ttctagcgtc
     7621 agacgattat tctccaatca aaaactggat gtcagagcat atccatcaat atggtgcatc
     7681 tagaaaacca aatcaattaa tcatggatgc aacacatgaa ccgcttaatc cgcaatacct
     7741 gatcgactat atgaaagacc tgtattttaa tgtatatcag gtaaaataaa atacaattta
     7801 caaagatagc tgaaattcat ctaatctata gatgttttca gctatttact taagaaagaa
     7861 gtggaacgaa tggataaatt acaagtgatc gctgattata gtatggacca attagcgcat
     7921 gatcaaacag gacatgggag cgatcatacc aaaagagttg tcaagttagc tgaaaaaatt
     7981 ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg cagcagctta cctacatgat
     8041 acgattgatg ataaagtcgt taaaaatgag aacgaggcaa agcaacagct tcgtgtattc
     8101 cttcgctctt tgccaatagt agaggaacag atctcgatga ttttttccat tatcgaaaat
     8161 atgtcctttt ctaaaaatct ctctgaagcg gccgaactat ctctggaagg taagatcgtt
     8221 caagacgctg atcgtatcga agcattagga gcaatcggca ttttgcgtac tgcttatttc
     8281 ggcggtggac atgggcaccc gatttttgat cctgaactgt atccgcaaac attcaaggac
     8341 aaagaaaatt accgaaaagg cacgacagtg atcaatcatt tttatgaaaa actgttcttg
     8401 ctgtctgata aaatgaatac tgattacgga tatgaagaag ccaaacgaag agaaaccttc
     8461 atgcgtgaat tcttagaaga attttttctg gaatggtcta ttgacgatga aaattttact
     8521 ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt tgtgacagaa gtggtcagct
     8581 ccaagatatt tcggatcatt ttccgtaaaa gttacttctg gaacactgtt taccaagagg
     8641 tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaat agcttctcgt
     8701 gtttgatgaa tattgtaact tgtgattcgg aattggcagg agaacaccgt ttattcggaa
     8761 gaataggact gtgacaagac ttttgtcaca gtcctttttt cgaattcaac aagtaaaact
     8821 tttaagaaat attcagagag taaaattaaa aattgctgat aaacgaagat ggaaatttta
     8881 tgtattttta ttggtctatg caataaataa attaaatatt cagaatacaa ttgattttaa
     8941 cgaaagggct tctatcaatg gttgtgttgt atttatctgc ccaggtagag taaaatagat
     9001 cattatctaa aaggtaattg ttcatactat ataagtcatt tgaaagtaaa aagatactgt
     9061 aaataaaaaa tgtggttaaa taattctcat aatcagtgta agcgttatca aaaatatagc
     9121 atacttgctt tgtagatgaa tgacacgata tatgtttggt atgttctgca agacagatga
     9181 atatggaggt ggagacaatg tcttccaaag aactaacaat gtatttagag aaagaattgg
     9241 atcaactgaa agaaaagggg ttatacaata cgatcgacat actagaaagt gaaaatggtg
     9301 cttgtatcat cgtagacgga aaaaagatga tcaatctagc atccaacaat tatcttggat
     9361 ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc aaccgaaact tatggtgtag
     9421 gagcaggagc agtccgaacg atcaatggtt cgctgaaaat ccatcagcaa ttagaagaaa
     9481 aaatcgcaga attcaaaggg acagaagcag caatcgcctt tcaatctgga ttcaactgca
     9541 atatgggcgc tatctctgca gtaatgacaa aagaagatgc tatcttatcg gatgaattaa
     9601 accatgcatc tattattgac ggttgccgct tatccggagc aaaaatcatc cgaattaaac
     9661 atcaagatat gaaagacctt gagaaaaaag caaaggaagc gatagaaagt aagaaatata
     9721 aaaaaatcat gtatatcaca gatggtgttt tctccatgga tggagacatt gcacgattgc
     9781 cggaaattat tccgattgtt gaaaaatatg gactgatcac ctacgtcgat gatgcgcacg
     9841 gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca ttttggatta agcgacaaaa
     9901 tcgatatgca aatgggcacg ttatcgaaag ctataggtgt tgttgggggt tatgtagctg
     9961 gatcgaaaac actaatcgac tggttgaaag ctagaagtcg gccattccta ttttctactt
    10021 cgctaactcc aggtgcagct gcagctgcat tggcttcgat tactttgatg caagaacatc
    10081 cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt taaagaagaa ttgaaaaaag
    10141 taggctataa catcggtatg tctgaaacac ctattactcc agtcattttg ggagacgaaa
    10201 aagtgactca gactttctct aagaagctga tcgaacatgg gatttatgct aaaccaatcg
    10261 tttatccaac tgtgcctctt gggacaggca ggatcagaaa tatgccgact gcggaacata
    10321 cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa aataggaaaa gaaatggaaa
    10381 ttatttaact aaatttagtt ttttcgtcta tttaaaatta gagaacatgt tcacgcatat
    10441 attgttaatc atctgaagat agtagtaaaa aagatcaggg aggatcatca tgaaaaaagt
    10501 attggtgaca ggatgtttag gacaaattgg ttcagagttg atcacacggc tacgtcaaga
    10561 actaggcagt gaaaatgtca ttgcgacaga tatacgcatg cctgaagtca ttgagaaaaa
    10621 cgatatcttt gagatcctag atgtaatgga ttacgaaaaa atgcgcagca tagcagaaca
    10681 atatcaagtg gacacattga ttcatttggc tgctcttttg tcagctgttg cagaagcaaa
    10741 accacaatta gcctggaatt tgaacgtgac tgggctggtc aatgcattgg aagtcgctag
    10801 agaattccag ctgaaatttt ttacgccaag ttcaatcggc gcgtttggtc caaatactcc
    10861 aaaagatcat accccacaag atactcttca gcgtcctaca accatgtatg gggtaacgaa
    10921 ggtagctgga gaactactat gtgattatta ttatgtgaaa tatggcgtag atactcgtgg
    10981 tgtccgtttc cctggattga tttcttatgc aaccttgcca ggaggcggta cgacagacta
    11041 cgcagtcgat atttattatt cagcaatcaa agaaggaaag tacactagtt tcattgatca
    11101 aggtacatca atggatatga tgtatatgcc agatgcgatc gatgccatta taaaactcat
    11161 gaatgcagat cctgatcgat tggttcatcg aaatgccttc aatatatcgg cgatgtcttt
    11221 tgacccggaa gagatcaaag catctattca aaaaatcata ccagattttg aaatggatta
    11281 tgatgtggat cctgtacgtc agtcaatagc agaatcttgg ccaaattcac tagatgtttc
    11341 ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac ttagatgtga tgacaaagga
    11401 tatgttggaa aaactgaaag aaaaattggt agagacggta taaaacaaga accacatcca
    11461 aaaagttttt tatacttttg ggattggcta tggtatgaaa ggggacaagg gatggaagaa
    11521 tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg aattaaaacg tacgatggga
    11581 ttttttactg ctttatcgac tgttatgggc acagtgatcg gtgcaggggt attttttaaa
    11641 gccgcaagcg tagcagaagt tacaggatcc gccagtttgc atatgttttc ttggtttctc
    11701 ggtggtatga tttctgtctg tgcaggacta acaggagctg aactggcagc tgctattcca
    11761 gaaacaggag ggatgattaa atatatcgaa cggatttacg gaaatacagc agctttttta
    11821 ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg tagcagcctt gtcgatcatt
    11881 ttcggtacac agttcgtcaa tctatttggt ctttcccagt caatgattgt tcccgtagct
    11941 gttacagctg ctgtatcgat cctgttgatc aattttttag gttctaaagc tggaggggct
    12001 tttcagtcga ttacgcttgt ctgcaagctt attcctttgt ttgtgatcgt cattttcggt
    12061 ttgttccgac aagaaggagt agacttccaa ctgtttccga tccaagcagg agaaaatctt
    12121 tcatttttct cagccttggg agcgggcttg ctagcaacga tgttcgcata cgacggttgg
    12181 atacacgtag gaaatatttc gggagaattg aaaaagccgg cgaaagattt gccaaaagca
    12241 atttctttag gaatcatcgg aatcatgatc gtttatttat tagtaaatgc ggtgttctta
    12301 agaaccgcat ctatcgacgg agtcgcagga aacagcaatg cggcaagtga cgttgcaaaa
    12361 atgatttttg gcggcttcgg tggaagactg gtcacagttg gtatcttgat ttctgtttat
    12421 ggaaccatca atggctatac ccttactgga atgcgtctcc cttatgttat ggcaaaagaa
    12481 aacaacctgc cttttagcaa gctctttgct aaacttcatg ataaaactaa agttcctgta
    12541 gccgcaggta tcttagagct agtcatcgct attggcatga tgatgatcgg tggttttgat
    12601 acactaacgg atatgctgat ctttgtcatt tggatttttt atacgatggt ctttgttggt
    12661 gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac catacaaagt tccaatgtat
    12721 ccgtttattc cactagtagc aatcattgga gggactttta tcgtatcaag cacgcttatc
    12781 actcagacct ttttagcttc gatggggata gtattgacac ttgctggtat cccgatttat
    12841 ctctatttaa aacgtagata gctaaaacat tcgaatttta ttagaactgc acttctctgc
    12901 caaacaaaat attttattta aatgaggagt acgtggaaga aatgtcaaaa gtactcgtta
    12961 attttgatca cgtaaatgtc gttttgtcta cgatgaaata gcaaagtaaa agacagaaat
    13021 cgggctcaac cgatttctgt cttttacttt gatttgcgta actgaactat tcggcgtacg
    13081 ccgcgataaa tatttaataa aaccaagcaa ccagaaatga aaatgggaag ccaaatcaga
    13141 tagacagggt agccaagcac tttagacaaa gcatgtggag tcattttatc catttcaaga
    13201 agtgcttgct gccaagtctg aagattgaag actagcatga aacccgaaat aatcaggaga
    13261 ataaatccta aataatgaat tgtagttctt tttcttacat aagcgatcgt agcataaagg
    13321 aacagtaaaa aaataggtac gccaaaaaca aagtacatat tcagcatctc gacaccccct
    13381 ttgtgtttat cttactttac tttgaaagaa atgagaaata atagcctcgt tacttgcttg
    13441 aaacagcaaa ggactgctaa gataagagag atacaaagaa tcaaggagga gtcatatggc
    13501 aagttatatc gaaccgattc gttttgcgct gattgttttt ccatttttag ccttagcgat
    13561 ctcatctgtt ttttttattt atgaatatcg aaagtatgga acatttctag taacaagggc
    13621 agttattttg tattctttcg ttttttattt attgtgtgcg tattttttgg tgatcttacc
    13681 acttccggca aaggagacgg ttgcccaaat gacagggcca aagatggagc tgcatttatt
    13741 tgcttcatgg caacatttca gagacaacac ggtattggtc ttgtctgaac ctagtaccta
    13801 cctgccggca atgaagcaga gtgtattttt ggaaccagct tttaatgttt tattactcat
    13861 accatttggc atttatttaa gatattattt ccggctttct tttatgaaaa cgatgattgc
    13921 tagtttctgc ctttctctat tttttgaatt gacgcagcta agtggacttt attttatcta
    13981 cccaagacca taccgcttat ttgatgtgaa tgatttagtg acaaatacat tgggtggaat
    14041 cattggtttt gtcgttacgc cgttatttac atttatgctt cctacaagaa gacgaatgga
    14101 tgaactttct tatgaaaaag gaaagactgt ctcattgact cgacgcttgg tcgcttattt
    14161 gatcgactgg acatttttat ctcttattaa tttggtagcg gcgattttct ttaaaattgc
    14221 gttcgattca ggggcagtta cttctcatat ctggtggctt ttattagaag taggggtata
    14281 ctttatgatt gtaccgttta taaccaatgg atggacactt ggcaaaaaaa ttgtgcgtat
    14341 ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa gcattgatga tccgctacgg
    14401 gtatctgtac tttctttttt atggtttaag ctggttgggg cagttgcttc agcaaatcat
    14461 gaattcgaca acgaatacgg tgttgcagat gagtgcagta ttcatttctc tcggtgtctt
    14521 gttactacaa ggtattttta ttttaaatat cttgctatca tttttgagaa aaaaacggat
    14581 tctgttttat gaaaaagcta gtcacacata tactgttagc acgattggcc aaacaaagga
    14641 tgatgattct cgaaattagc tgagcaaaaa cgctttgtct gtgttataat gccaaagtcg
    14701 tgataattta aagagaagag gtgcacgaat gaataatttt gatatattcc gcttcaaacc
    14761 gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag cccacagagg tccaggaacg
    14821 tttgattcct ttaattgcaa aaggaaaaaa tatcattggg caatctcaaa ctggtacagg
    14881 aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc aatgcagaaa aagaggaagt
    14941 acaagttgtt attacagctc caagtaggga actagctgca cagatctatg aagcagctac
    15001 acaaatcgct aaattttcta caccggagat ccgtgtctcg aattttgtcg gcggtacaga
    15061 caaacagcgt caattaaatc gtctgcatca ccaacagcca caggttgtag taggcacgcc
    15121 tggtagaata ttagacatga tgaatgaaca agcgctgaag attcacaccg catttgcatt
    15181 tgttgtcgat gaagcggata tgacattgga tatgggtttt ttaaatgaag tagaccaaat
    15241 tgcaagtcga ttaccagaaa agctgcagtt tttagttttt tctgccacta ttccagaaaa
    15301 acttcgtcca ttcttacgaa aatacatgga aaatccaatc attgaagaaa tcaaaccaaa
    15361 agcagttatt tctgaagaca ttgaaaattg gctgatttca acaaaaggta aagatgagaa
    15421 tcaaatgatt tatcaactgc tgacaattgg tcatccttat ttagcaatcg tatttgccaa
    15481 tacaaaacag catgtggatg aaattgccga ttacttgaaa aaccaaggct tgaaagtagc
    15541 aaagattcat ggagacatca ccccaagaga gagaaaacga gtgatgcgac aagtacagaa
    15601 tctggagtat caatatgttg tagcgactga tttagctgct agagggatcg atatcgaagg
    15661 tgtctctcat gttattaatg cagaaattcc agaagatcta gacttcttta tccatcgtgt
    15721 cggacgtact ggacgtaatg gcttaaaagg aacggccatt acgttatatt cgccaaatga
    15781 tgagcaagcc attgatgcga tagaaaaatt gggcgtctca tttgaaccaa aagaaatcaa
    15841 aaacggggaa atcgtcaaaa catacgatcg taatagacgt gccaaacgag aaaaatcaaa
    15901 agatgtatta gatcccacgc tgattggtct tgtgaagaag aaaaagaaaa agatcaagcc
    15961 tggttataag aaaaaaatcg attgggcaat tgcagaaaaa aataaaaaag aacgcaaaat
    16021 cgaacgcaga cagcaaagcc gtactgctcg taaaaataag aaaaattcac ataaataaaa
    16081 aacaagaggt tgtgacaaaa ggcgtgtcac aatctcttgt ttttaagcta gacttttttt
    16141 cattccgata caaagtactt catcttcaat aaaaggttct gtgcaagtat gatacccttg
    16201 tttttcataa aaatctacag ccgtcatttc agctactaca taactagtct gacaaccttc
    16261 tgaaaccgcc cgtttttcag cagcttcaag caactgaccg cctaaccctt ttcctcggta
    16321 ttcttttgct acgcaaaatc gttccggctt gaagcaagta ggtgttttct tttcataacg
    16381 caaagtagct actggtcttt tttgatcaag aaatagataa taggtttgtt gcgggatatc
    16441 ttttccgtcg aattctagtt ctgcaggaat cccttgttct aatacaaaaa cttgatatct
    16501 caaaaagcag ctagctgcgc gtatccattc atccgctcca actatttttt tcatcatatt
    16561 cctccttgtc atgaatagca gagatgtatt gattccttta ttcttatcgt accaaaaatc
    16621 gtggagtatg accaaaagga aatgaaaata aaaattgaga tattgacagt gatcaataga
    16681 atgcgtatac taattaagta cgaaaaggat atgatttgtt ttttaagact cacagaaaat
    16741 tctttcttgg ctgaaaaaag aatagtcact aatcaaattg ctcccttttt ctattttggt
    16801 agaaataccg acgcagctca gcgttatcga gtttaagagg taatgattac acatcattgc
    16861 aatcaaggtg gtaccgcgag ttttcgtcct tggctgtgat ggtgtttttt tgtatttaat
    16921 ttatagaaag caggtaatga catgaaacaa ttaagtagtg cacaagttcg acaaatgttt
    16981 cttgatttct tccaatctaa aggacatacg attgaaccta gtgcgtcttt gatccccgtg
    17041 aacgatccaa ccttattgtg gatcaactct ggtgttgcaa cattgaaaaa atattttgat
    17101 ggttcagttg tgccagaaaa tccaagaatc acaaatgccc aaaaatctat tcgaacaaat
    17161 gatatcgaaa atgtcggtaa aacagcacgg caccatacga tgtttgagat gctgggtaat
    17221 ttttcgattg gtgactattt caaaaatgaa gccatccact gggcatggga atttttgact
    17281 gatccaaaat ggatggcatt cgatccagaa aaactttatg taacagttta tccaaaagat
    17341 acagaagcaa aacgcatctg gcatgaagaa gttggcctgc cactagatca cattattgat
    17401 gtagaagaca acttctggga tatcggtgca ggcccatgtg gtccggatac ggaaattttc
    17461 tatgaccgtg gagaagctta taacgattta gcagaagacg atcctgaaaa ctatccagga
    17521 ggagaaaatg aacgttattt agaaatctgg aacttagtat tttcagaatt caaccataag
    17581 cctgataaca catttgaacc acttcctcat aaaaacattg atacggggat gggattggaa
    17641 cgcatggtct caatcgttca agatgcgtca acgaactttg agacagactt gtttatgcct
    17701 attatccacg ccgtagaaaa gctaagcaaa caagtaaaat atggtgaaaa cccagtaaca
    17761 gatatttctt acaaagtaat cgcagatcat attcgcgccc tttcatttgc tattggcgac
    17821 ggtgcattgc catcgaatga agggagaggg tatgtcttac gccgtttgct tcgtcgtgct
    17881 gtcatgcatg ggaaaaaatt aggtattgaa caagcattct tatatcgttt agtccctgtt
    17941 gttggggaaa tcatggtaag ttattatcct gaagtacttc aacaaaaaga atttattgaa
    18001 aaagttatcc gtacagaaga agaacgtttc aatgaaacaa tcaatgaagg tttggaaatc
    18061 ttaaatcaag tgatccaaga agtaaaaaac gaaaacggga cgactctagc aggaaaagac
    18121 atcttcaaac tatacgacac atatggcttc ccggtagaat taacggaaga ggtggcagaa
    18181 gatgaaggac tgaaagttga tcatgctggt tttgaaaaag aaatggatgc acaacgcgaa
    18241 cgtgctcgct ctgcccgcag tactgaaaaa tcaatggggg tacaatctgc tttattgaca
    18301 gatatcaaag tagaaagtaa attcattgga tacacagaac aagaatcaga tagtcaatta
    18361 ttggtcatcg ttaaaggtga gtctcttttg aatgaagtcg cagaaggtga agctcaaatg
    18421 atctttgatc aaacaccttt ctatgcagaa atgggtgggc aagtagcgga tcaaggatgg
    18481 atcatgaatg aagtaggcga aatcgtagca gttgttacag atgttcaaaa agcgcctaat
    18541 ggtcaattta tgcacaccgt agaagtaaaa gcaccaatga gtgaaggaca aacctatcat
    18601 ctattgatcg atcaatcgat gcgcaatcgg atcatcaaaa accatacagc aacccatcta
    18661 ctgcacaagg cattgaaaga agtacttggc gagcatgcaa atcaagctgg ttctttagtc
    18721 gctcctggac atttacgatt cgactttacc cactttggtc aaatcacttc agatgaatta
    18781 gctgaaatgg aaagaatcgt caacgaaaaa atctgggaag ctatcccagt tgaaacaatt
    18841 gaaacagata tcgatacagc caaaaacatg ggtgcaatgg ctttgtttgg tgaaaaatac
    18901 ggacatgacg ttcgagtagt aaatattggc ggctggtcaa tcgaactgtg cggaggcaca
    18961 catgtagcaa atacagaaaa catcggaatc ttcaaaatcg tttctgaatc aggaattggt
    19021 gcaggcgtgc gacgaatcga ggctgtaaca agtaaagaag cgtacgaact acttgaagaa
    19081 gaagaaaaac aactaaaaca agtagcaaca attgttaaat cacctcaatt gaaagaagtt
    19141 gtttcaaaag cggaacaatt gcaacagcaa gtaagagagc tacaaaagga aaatgaacaa
    19201 ttagctagca agttagcgaa tcaacaagct ggtgatgtat tcaaagatcc agaaacgatc
    19261 aatggaaaaa caatcattgc agcacaagta aaagtgaaag atatgaatca gctgcgtcaa
    19321 ttagctgacc aatggaaaca aaaaaatatt tcagatattc ttgttttagc aaacgaacaa
    19381 gaaggaaaag tcagtctgct agcggcaatg acgaaagaat caaatgaagc tggactaaaa
    19441 gctggcgact tgattaaagc aatcgcaccg aaagtaggcg gtggcggtgg tggacgtcca
    19501 gacatggcac aagctggcgg gaaaaatcct gctggtattc cagatgcact taaagctgcg
    19561 aaagaatggg ttgcgcaaca atagggcgtt tacgtttgac agaaaaacaa aagttcaaac
    19621 attgaacgaa acatgattat gggggcgaag acaaacaagt cttgccctca tacttttttc
    19681 aattggatgg cgcatctaat caaatggtcg gattcggatg gtctttagtt gacgaaagtg
    19741 acagaaattt ttacgattag tataaataaa aaggggtgaa aaaatgaata gtagaaataa
    19801 gacctttcga ctagtcttac gtgcgatctt gttagcaatc atcatcgtac aagcgatggt
    19861 tccttggctg gggtttattc cattaggatt cattagttta acaattatcc atatcacagt
    19921 aattattgct gcagtagtat taggcccaaa agatggcatg gtgattggct tgttttgggg
    19981 aatcgctaca attgttcgtg cctatgccat gccaacaacg ccatttgata cattagtgtt
    20041 cacgaatccg attatctcgg tagttcccag agtacttgtt ggcttagtag caggcttggt
    20101 tttccactgg atttatcaaa aaaataaatc aataacgatc agctcagcct ttgcaggggt
    20161 actgggttca ttggtcaata cagtcttagt gttaggattc atgggattat tttatacagg
    20221 tgcaacggca gatgcctatg gtgtagactc tgctctttta tttaaaacct tagcaggggt
    20281 tgcagcaatc aatgggatac cagaagcaat aggtgcggga atcataacac cattactagc
    20341 aaaagcgtta tttgcagcaa caccgttgaa accagaataa tgaccatctt ttaatagtaa
    20401 taggacgcac gatagataga aaaaggaaga gaggagagga aaacaatcgt cctctccatc
    20461 tttttacctg agcaacaagc aaactagaag gggtaaaaaa cttggctaag aaacaagaag
    20521 aaaaaaagaa acaaataccg caacagcgga caaaaaaagg agtactagcg ggtatcttag
    20581 gattggttac gaatctgatc ttgtttgtct ctaagctaat gatcgggctt ttttcaggaa
    20641 gtgtctccat tatggctgat gcagtgaaca gtctatcaga tactgcttca tccatattga
    20701 ctttgatcgg atttaaaatt gctgcaaagc cagcagatca ggaacatcct tatggacacg
    20761 aacgcttcga atatatcagt gggttatttg tttcgatcat cattacttat gtaggatttc
    20821 aatttttaga gtcttctgct gaaaaaatca tccatccaga aaatgtctct ttaacaccca
    20881 tcgtcttttt tgtcctaatc atttccatct ttttgaaatt tgcacaaggc aaaatgtatc
    20941 aggttattgc gcaatccatt gattctgaaa cattgagagc tacagcaaca gatagttata
    21001 atgatgtctt tactacactg gctgtattgc tttcagcagc aatcgaatgg gtaactggat
    21061 ggcgtattga tggatatata ggttttgctt tagcattata tatcctttat agtggattga
    21121 cgatgcttcg aaattttgta tacgaactat taggaagtcg cccaacgcca gcagagattg
    21181 aagaaatgga agagcattta aatgcttgcg atgatatact tggctatcat gatttattgg
    21241 tgcataacta tggacccaat aaacgctttg catcagtcca catcgaagta gatgaatcaa
    21301 tgaatttgaa tgtggctcat aagattattg atctgataga aaaagacttt aaaaagtctt
    21361 tggatgtgga acttgtctgt cacttggatc caatgcctgt tgataacgaa aaatactata
    21421 atatcttgcg gcagttaaag caaattcttc ttgaaacagg tacagggctt aatattcatg
    21481 attttagagt aattcatcaa ggaaaaacgc tgcaatttga tgtagtggtc cctgagaaat
    21541 tccagtttgt tgatacagaa ctagaaagaa tcttacgtag tcaaatagaa gaaaaaatcg
    21601 gagattacca attggaaatt acgtttgatc acaattatct tttatattaa atgagaaact
    21661 ataaaaagac tccttaaagt ttttaattta aagagtcttt ttttctttta attgtttatg
    21721 gagagcctga cgtgcaagat tatctgctcc tttatttttg ttttcgggca accattgcag
    21781 gatcaataaa gaaaattcgg atagaagttc ttggattctt tgaaggtagg gaaggaagag
    21841 ctcgttcttc gttgcgtttt tgtcgaccgt agaaactagt gttttgctgt ctgaaaaaca
    21901 gaaaatcgtt tcattctgta agtcgttttt ctttagaaat tctaaagttt ttaaaaagac
    21961 ttcgaactct gcttgatggt ttgtccctat agaaagcggg atagatagct gttcttgttt
    22021 accttgatag acaatcaaga ttccacctcc actaggacca gggttgcctt ttgtagaggc
    22081 atcaatgtat acttttaaca taaaaagaga ttctcctttt tgttcaaact aatgttatgg
    22141 taagattata tcattactga atcggtgaga ggttagagaa tgaaaaaaaa tattcgatgg
    22201 cagcctgaac tggcacatac gatcatctat tggtcattga ccttgatcat tctgtttttt
    22261 agtctgattc tttctttaga aaacacaggt ccttattgga aaagcaattt tgttttaggt
    22321 atttttcttg tttttctatt tctaggtttg cggcgttctt ttattttaaa atcaaactct
    22381 gtgattatac gttatgctgc tttttggaaa gaccgagaaa tatctgtcgg acagattcga
    22441 tctttgacgc caactaatac tggggtgcaa atgatgttgc aagatgagaa agccccatct
    22501 gttttcatga tgcgaaaaaa agatcaagta gtcctttttg atcattttga aaaaaacgga
    22561 cccaaatcag aatcaattcc ttatccttat acgaaaaaaa gaaaaagcta attttaaaaa
    22621 tcagaaccta aaaaaacgaa tttttacagg aaaagaaaag agtgagacgt acggttcatc
    22681 ccgtttagcg tctcactcta tttgtttatc tattcatgca tactagtatc ggtagtctga
    22741 acgttgcctt tcttgacatg ggcagcttgt gttcctcgat tcccttcgat gatctcaaat
    22801 tcgaccactt gattttcttc caaagttttg aatccctctt cttcaatcgc agtaaaatgt
    22861 acaaaaattt cttctgtgtc atcataactg ataaagccgt atcctttttt gttgtcaaac
    22921 cattttacaa ttcctgtggt caaagcactc acgacctttc taaagtctga tcatagcctt
    22981 cattataaag agtatttata agtacgtcaa ataaattaaa aacctgtaat tttatgtttt
    23041 ttttctgaat aaaaaaaggt ataattggca tatcttaaca aagaagatat tttgttgaat
    23101 aaagttcgtg aaaaggggta atcaaatgaa gaatgatctg caaatttcac aagaaacaga
    23161 gctagaaccg attgttgaag tggctagtaa attagatctt aaggaagacg atttggaact
    23221 gtatgggaaa tataaggcga aaatcaattt ttctggaatc aatcgatcaa aggataacgc
    23281 tgaggggcat ttgatcttag ttacttccat caatccaaca ccagctggcg aaggaaagtc
    23341 aacgatcact gtagggttag gagacgcgct caataaaatc aataaaaaat ctgttattgc
    23401 attgcgagaa ccctcacttg gtcctgtgat gggaatcaaa ggtggtgctg caggcggagg
    23461 atatgcacag gtattgccta tggaagatat caacttgcat tttactggtg acatgcatgc
    23521 tatcacaaca gctaataatg cattatctgc tcttttagat aatcatattc atcaaggaaa
    23581 tgaattgaac atcgatgccc gccgagtgat ttggaaacgg gtagtcgatc tcaatgatcg
    23641 tgaactgcgt aaagtaattg ttggcttggg tgggccaatc caaggtgttc caagagaaga
    23701 tgggtttgat atcacagttg ccagcgaaat catggctatc ttgtgtcttg cggcagacct
    23761 tgaagattta aaggctcgtc ttgcaaggat cgttgtcggc tatacatttg accgtcagcc
    23821 tgtgactgct ggagacttga aagcagaagg cgctctggca ttactgctga aagatgcaat
    23881 caagccgaat cttgtacaaa caatttacgg aacaccagct tttgtccacg ggggaccgtt
    23941 tgccaatatc gctcatggct gcaatagtat tttagctacc aagacagcat tgagattagc
    24001 agattacgtt gtcacagagg caggatttgg tgcagatctt ggcggagaga aatttttaga
    24061 tatcaaagta cctaacttga aaaaagcgcc ggatgctgtg gtgattgtcg ccactgtccg
    24121 tgcattgaaa atgcatggcg gaatgaaaaa agatgaactt aaaaatgaga atcttgatgc
    24181 tttgaaagta ggttttgcaa atttaaagcg ccatatacgt aatatggaac aatatcagct
    24241 acctgtgatc gtcgcaatca atgaattcgt aacagataca gacaatgaac tcacactgtt
    24301 ggagcacttg tgtgaaaatc aggggattct tgccaagaga gcgagtgtat gggcagatgg
    24361 agcagaaggc ggcgttgatt tagcaaaagc tgtagtacga ttgattgata gaaaagaagc
    24421 taattacaag ccattatacc gcctagaaga aacgatacaa gaaaagacag aaacaatcgt
    24481 caagaaaatt tacggaggaa atggtgttgt tttcagcaaa aaagcgcaaa aacaaataga
    24541 agagtttacg aagaatggat gggacaaact gccgatttgt atggcaaaaa cacagtattc
    24601 attttctgat gatccttcac tgctaggtgc accggaagat ttcaccatca cgatccgcga
    24661 aatcatccca aaattagggg caggattttt agtagccttg actggagatg ttatgacgat
    24721 gcctggtttg cctaaaaaac ctgctgcatt gaatatggac gtgacaaatg acggagaagt
    24781 gttgggattg ttttaagctt gattataatc taccatcgaa ggagctgtat tttgactaca
    24841 gcttcttttt ttagacgata gggaattaat tttgtttaca ctaggatcat tctttttctt
    24901 tattgtattt tgagctatac taaagcataa taggagggta tgatgaaaac gacatttata
    24961 agaaaaaccg gtttttacgg tatgggtagc tcgattcagc taagaatcaa caatgaaaaa
    25021 cgcagtgttc tcagtcatga acaaaagata acgcttgatc tggaagtgcc ctttacgatg
    25081 caggtcacct tttattggct aaaaagcccc gtttatacag tgaaagaagc aaagcctgct
    25141 tatgtgatca caatgaatta cttgattctt caaatctatc cgtttttatt tctagcaact
    25201 gggttggggg ccgttttcct acaaaattta tttttcagtt tttttgcagt tattgtgttg
    25261 attgcgttct ttttctatat aaaaaaccgg gcatatttga tcaaggagga aaattatggc
    25321 aaactctgat ctttttttaa tcagtttcaa tcgaatcgaa aaatggatga gagacgagat
    25381 gggaaatgca cgaaatatgg gatttaccga acttgtcaga cgcctagctc aaagaaaaca
    25441 attaatgatc agaaaatacg aagatgatct tttacagctt gctcagttaa ggaatgcgat
    25501 cgtccatgat cggatcgcag tcgattttgt cattgcagag ccaaatgatt ggacagtcaa
    25561 gcgaatccag ctgatcgaac aagaactgat tcagcctgaa accgtgctgc ctcgatttgc
    25621 aaaacgtgta acaggttttg aacaagatct tccgttactt gaactgttaa agatagtagc
    25681 agaaaaaagg tattcacaat ttccgttata caataaagga aaatttgtag cattgattac
    25741 tttacgtaat atcgggtttt ggctagcaaa agaaagccaa aaaggtccgg ttgatttaac
    25801 aaataaacga gcgttggatt tgatcatcca aaacggaaag tacacaaatt atcattttgt
    25861 tccagctaaa acacatattt acgaggttga agcgatgttt cgtgagcaag ggactttgga
    25921 agccgtactt atcactaaag atgggaatcc cgatggtaat ctgctcggga tcgtacgacc
    25981 tcgtgatatt tataaacaaa tagaaaagga ttgatttttt gttaattatt tattggatat
    26041 tcagcgcttt gctagtagga gtggatcagt tggtcaaatg gtggatcact gataatctag
    26101 cgcttggaga gacgaaaaat cttataccag gtattttgtc actgaatcat attcgaaaca
    26161 ctggagccgc atggagtatg ctagaaggga agatgtggtt ctttactata gtgacactca
    26221 ttgcagtcgt tgtgattctc acattgatga ttaaaaatcg tgaaaaaggt aatcgctggt
    26281 tcatgattgg attgagtttg attctagctg gtgcgattgg caattttatc gaccgtattc
    26341 gtcttggcta tgtagtagat atgttccaga cagattttat gagctttccg atttttaacg
    26401 tagcagatgt gacacttgta tgtggagtga tttgtatgtt aatttatatt attttagatg
    26461 aaaaggatca aagaaaaaaa tgacagaact taaaacgacg attcaccatg aatctggtag
    26521 gatcgataaa gtattgacta atttattcac cgattacagt cgttcgcaaa ttcagctgtg
    26581 gctgaaaaat ggagcggttt ctgtcaatgg acagccagtc aaagcaaatt acaaagtaaa
    26641 acaaggagac gagattactg tcttgatccc cgaaccagaa acattagatg tggtcccaga
    26701 aaatatcccg ttgacgatcg tttacgaaga tgaagatgta gcggtgataa acaaacctca
    26761 aggaatggtc gttcatcctt cagcaggtca tacgcatggc acaatggtca atgcgctgat
    26821 gtaccatatc aaggatcttt ctacgatcaa cggcgttatt cgtccaggta ttgttcaccg
    26881 aatcgacaaa gatacttccg gactgctaat ggttgccaaa aatgaccagg cacacgaatc
    26941 attagctcag caattaaaag ataagacctc tttgcgcaaa tatgttgctt tagtccatgg
    27001 agagattcct cacgaaaaag gaaccatcaa tgcgccaatc ggccgttcga aggaaaaccg
    27061 gaaaatgcag gcgataagag aagatgggaa gtcggctgtc acacatttta ctgtattgga
    27121 acgtttcgaa ggatttactc tggttgaatt aaaactagaa accggaagaa cacaccagat
    27181 ccgtgtgcat atgaagtata tcggttatcc gttagctgga gatccagtat acggaccgaa
    27241 gaaaactttg aaaggcaatg gtcagtttct ccatgcgcaa atgttaggtt ttacgcaccc
    27301 gactacaaac gaaaagatga tctttgaagc acctcttcca gaagtttttg aaaaaacttt
    27361 agaaaaatta agaaaaaaca ttgcatttta atttagataa cggtatagta attaacaacg
    27421 aaagcataaa acttaataga attcctttaa gtttagtccc gtgaggctaa gaagggtggg
    27481 agcaaggata cgtatggtgc gccataagta gattgctcat tgacatagta tagctatcag
    27541 tgtatcagta tacgctcagt ctaaacattc ctcctgtcct cttagggcag gagttttttt
    27601 atgcaaaaaa gtagggggaa tgaaaatgca agcaaaagaa gtagtagatc aggtaaccat
    27661 gaagcgagca ttgactcgaa ttacttatga gattatcgag agaaaccata gtatccaaga
    27721 tatcgtattg gtaggtatca aaacacgagg aatctacatt gcatcaagaa ttgccgaacg
    27781 cctgaagcag ctagaagata tcgatattcc tgtaggagaa ctggatatca ctttataccg
    27841 agatgacaaa aaagaaaatc cagaagaacc ggaacttcat tcttctgaca tcccagtttc
    27901 cttagaagga aaagaagtta ttttgattga tgatgttttg tatacaggac gaacgattcg
    27961 agcagcaatg gacgcagtaa tggattttgg cagaccaaga aaaatttcat tagcagtcct
    28021 ggtcgatcgt gggcatcgag aattgccaat ccgtgcagat tatgttggga agaacattcc
    28081 aacatctcgg gcggaagaga ttttagtaga gatgcaagag cttgatggac aagatcgcat
    28141 catgattttg aaagaggaag attagaggag agagaaacat gtcggagaaa aaaggatttc
    28201 ataatgatga cgtggtgtta gatatccacg atcgcccaac cccaggacat tgggtaggat
    28261 taagtttaca acacttgttc acgatgttcg gagcaaccgt gttagtcccc atcttagtag
    28321 gtatcgatcc aggtattgcg ctagtcagtt caggactagg tacattggtg tacttattta
    28381 ctactaaagg aaagatacct gcgtacttag gaagcagctt cgctttcatt gcagcgatgc
    28441 agcttttgat gaaaacagat ggttatccag cgattgcaca aggagcagtc acaactggat
    28501 tagtctattt gattgttgcg ctgatcatca aaaagatcgg ttcagcatgg ctggataaaa
    28561 tcttgcctcc aatcgtcgtc ggtccagtca tcatggttat cgggttggga cttgcggcaa
    28621 atgcagcaac aaatgctatg tataatcagg gcacgtatga tttcaagtat attgctgtag
    28681 cgctgatcac attaggatta acaattttct tcaacatgtg gctaagaggt tttctcggat
    28741 tgattccgat tttactaggt attattagcg gatacatcgt agccttgctg tttggaatcg
    28801 tggatactaa gccaatcgta gacgctgcct ggtttgctgt tcctaacttt gagattccct
    28861 ttgtacaata tgaaccaaag ctatatcttg gagcaatcac aacaatggca cccatcgctt
    28921 ttgtgacgat gacagagcat atcggccatc ttatggtatt gaataaacta actaaacgta
    28981 atttctttga agaaccgggt cttcataaga cgttgacagg tgatggatta gcacaaatcg
    29041 tggcagcctt tgtcggaggt cctcctgtaa caagttacgg tgaaaacatt ggtgttctgg
    29101 ctatcacacg tgtccatagc gtgtttgtga tcggtggagc tgcagtattt gcagtcattt
    29161 taggattcgt aggaaaatta agcgctttga ttttaagtat ccctggacca gtcatctcag
    29221 gaatcagttt tatcttattt ggagtaattg ccgcaagtgg aatgaagatt ttaatcgaga
    29281 acaaaatcga ttttgataaa aagaaaaatc tgttgattgc ttcagttatc ttagtagtag
    29341 gaattggcgg actcgtcttt gaagtaggca catttacatt gtcggcaatg gcattagcaa
    29401 ctgtacttgg catcatttta aatcttatct taccagaaac ttcacgaagc gaagaaaact
    29461 aaggaggaaa cattatgatc atcaaatcag aacgtatcag cttaaagcat ttattgaccg
    29521 ttgaggcact tactgatcaa gaagttatgg gcttgatccg cagagggcag gaattcaagc
    29581 aaggtgctaa ttggactccg caaaagcgcc agtactttgc tacaaacctc tttttcgaaa
    29641 atagtactcg cacacataaa agcttcgatg tagcggagaa aaaactaggg ctagaagtga
    29701 ttgaatttga agcaagcaca agctctgtcc aaaaagggga aacactatac gatacagtac
    29761 tgacgatgtc agcattaggc gtagacgtag cggttatccg tcatggtgat gagaactatt
    29821 atgacgaact gatacaaagt aaaacaatcc agtgttcgat tatcaatgga ggagacggaa
    29881 gcggacagca tccaacccaa tgcttgcttg atttgatgac catttatgaa gaatttggtc
    29941 attttgaagg tctgaaagtt gccatcgtag gtgatatcac tcactctcgc gtagctaaat
    30001 ccaatatgca gatgttaaaa cgtctaggcg cacaagtatt cttctcaggt cctagagaat
    30061 ggtacgacga tgaattcgaa gtgtacggac attatatgcc attggatgaa ctactagatc
    30121 aagttgatgt catgatgatg cttcgggtgc aacacgaacg ccatgatgga aaagaaagtt
    30181 tctcaaaaga aggctaccat caggaacatg ggttaacagt tgagcgtgcg aagaagttgc
    30241 aaaaacatgc aatcattatg cacccagcac cagttaaccg tgatgtagaa ttagctgatt
    30301 cattagtgga aggattacag tcgcggatcg ttcagcaaat gagcaatgga gtattcgtca
    30361 gaatggcaat cttagaagca gtgctaaatg gaaaagcgta attataggag gatgaaaaaa
    30421 tgaaaacact cattaaaaac ggtcagatca atacacgaaa aaatatgacg acaccagcag
    30481 aaatttggat agaagatggg cgaatcaaag caatcggtac aggattctca gaagccgaat
    30541 ttgatgaagt gtttgatgca aagggacaat tgatcacacc tggtttagtg gacgtccatg
    30601 ttcatttgag agagccagga tttacataca aggagacgat cgaagctggg actcgctcag
    30661 ctgctcgtgg cggtttcact actgtatgcg cgatgccgaa tttaaatcct gtacctgata
    30721 cggcagaaaa gctgagacaa gtatatgaca tcattagaaa agatgcagtt gtgaaggtat
    30781 tgcagtatgc gccaatcaca gaaaatctgc gaagtgagaa attagttgat caagaagcac
    30841 tgattgaaga aggtgctttt gctttcacaa atgacggtgt aggtgtccaa acagctggga
    30901 cgatgtatct ggctatgaaa gaagctgcaa aaaacaataa agccctagta gcccatacag
    30961 aagatgaatc tttattattt ggcggcgtta tgcacgctgg caagaaggca gaagaacttg
    31021 gtcttcccgg gatcttaagc gtaaccgagt cttctcaaat tgctagagat ctgttgctag
    31081 ctgaagcaac tggtgttcat taccatgttt gccatgtttc tactaaagaa agtgtccgtg
    31141 taatcagaga cgcaaaaaaa gcaggaatcc acgtaacagc agaagtttca ccccatcatc
    31201 ttattttgat cgacgaagat attcccgaag atttcggctt ttggaaaatg aacccgccgc
    31261 taagaggacg ggaggacaga gaagctttga tcgaaggctt gcttgacggt acgatcgatt
    31321 gcatcgctac agaccatgcc ccccatggat tagaagaaaa aagccaaagt ttcatgaaat
    31381 caccatttgg tatcgtagga agcgaaacag cgtttcaact gatctatact cattttgtag
    31441 aaactggtcg attcacgttg gaacaagtga tcaattggtt ggcagttaaa ccggctgaga
    31501 tttttggttt gaatgcagga acgctaacgg ttggtgcccc cgctgatgta gcagtttttg
    31561 atattacaca aacttgcacg atcgataaag aagacttttt atcaaaaggg gagaatacac
    31621 cttttatcgg ctggaaagta aaaggcgaaa cacaaatgac ttttgtcaac ggaaaacttg
    31681 tgtggcagaa gggagaataa aagtatggaa cgtttgctta tattagaaga tggaacagtt
    31741 ttcgaaggaa aggcttttgg tgcagatgct agtgtcttcg gagaaatcgt ttttacaaca
    31801 agtatgactg gttatcaaga aactatcact gatcaaagtt ttaacggtca aatcattaca
    31861 tttacctatc ctatggtggg taattatggg gtaaatagag atgattatga atcgatttct
    31921 ccaacatgca aaggagtagt agtaaaagaa catgcacgtg tagcttcaaa ctggcgaaac
    31981 caaatgacgc tagattcatt tttgaaacaa aaaggaattc ctggaatatc aggtatcgat
    32041 acgcgcgccc ttactcgcaa aatccgtgaa gtcggaacga tgaaagctag tatagttgat
    32101 gctggagatt ccttcgaaca tgcatttgat cagctgaaag caaccgttct agcaaccaat
    32161 caagtgcagc aagtctcaac taacaaacct tatccaagtc caggaacagg tcggaaagtc
    32221 attgtcattg acttcggctt gaaacatagt attttacgag aattgagtaa aagaaactgt
    32281 aacctcacag tacttcccta taacacagat gccgagacga ttttagcgct atcaccagac
    32341 ggagtcatgc tgacgaatgg acctggtgat ccaaaagatg tgccagaagc aatcgaaatg
    32401 atcaaaaaaa tccaaggaaa agtaccgatt tttggtattt gtctcggtca ccagttattt
    32461 gctttagcca acggtgcaga tacctataaa atgaaattcg gacatcgagg attaaatcac
    32521 ccggtacgag aagtagcgac cggaagaatt gattttactt ctcaaaatca tggctatgca
    32581 gtcgatgaag ccacgattga tccagaaaaa ctcatcgtaa ctcatgtaga agtaaatgac
    32641 ggaacagtag aaggagtcaa acacagagat tatcccgctt tcagtgtgca gtttcatcca
    32701 gatgctgccc ctggtccgca tgatgcacta catttatttg atgaatttat ggaaatgatg
    32761 gacgcaggaa aggagcagta aacagatgcc gaaacgtaca gatatcaaga aaattatggt
    32821 gattggatct ggtccgatca tcattggtca agccgcagaa tttgactacg caggaacaca
    32881 agcctgcctt gccttaaaag aagaaggata tgaagtggtt ttagtaaatt ctaatcctgc
    32941 gacgatcatg acggataaag aagtggcaga tcaagtatat atcgaaccga tcacacttga
    33001 atttgtatca aggattcttc gaaaagagcg tccagatgct ctccttccaa ctttaggcgg
    33061 acagacaggc ctcaatatgg caatggagct ggcagcatca ggtattttag aagaattgaa
    33121 catagaactt ctaggaacaa agctttctgc gatcgatcaa gcagaagatc gtgatctatt
    33181 caaacagttg atgaaagaat tgaatcagcc aatccccgag tctgaaatcg tcaatacagt
    33241 agaagaagct gtagattttg caaataaaat cggctatcct gtcatcgttc gtccagcctt
    33301 tactttaggc ggaactggtg gaggtatgtg tgaaaatgaa gaagaattac gtatcgtatc
    33361 agaaaatgga ttaagtcttt cccccgctac ccaatgtttg attgaacgaa gtattgcagg
    33421 ttttaaagaa attgaatatg aagtgatgag agattcggct gataatgcaa tcgttgtctg
    33481 caatatggaa aattttgacc cggtgggtat ccatacaggt gattctatcg tatttgcccc
    33541 tagtcaaaca ttgtctgacc atgaatatca aatgcttcgt gatgcttctc tttcgattat
    33601 tcgagcactt aagatcgagg gaggctgtaa tgtccaactc gctcttgatc cacatagttt
    33661 taattattat gtgattgaag tcaatccgcg tgtttctcgt tcatctgcgt tagcaagtaa
    33721 ggcgactggc tatccgatcg ctaagctagc tgctaaaata gcgcttggat taacattaga
    33781 cgaaatgaaa aatcctgtta caggaacgac ttttgcagaa tttgaacctg cattagatta
    33841 tgttgtagct aaaattcctc gctggccgtt tgataagttt gaaaacggag aacggctttt
    33901 aggcacgcag atgaaagcaa ctggtgaagt gatggctatc ggtcggaata tcgaagagtc
    33961 tttattgaaa gctgtgcgtt ctttagaaat cggtacacac catatcgaac ttcctgagtt
    34021 ggcagacatc acggacgatg acctaatcca aaaagtggtt cgagcacaag atgatcgcct
    34081 gttttatgta gctgaagcta ttagaagagg gtacaccata gaagaattag cggatttgac
    34141 aaaaatcgat ttattctttt tagataaact gttgcatatc gtagaaatcg aacagcagtt
    34201 gaaacagcag ccgttggatg acaagatatt gaaaacagcg aaacaaaacg gttttacaga
    34261 caaaaaagtg gcggagctgt gggaaataaa cgaagaagcc gttcgccaga tgagaaaaga
    34321 aaatgatttg attcctgtat ataagatggt tgacacttgt gcagcggaat tcgaatcaaa
    34381 aacaccttat ttttatagtt cctatgaata tgaaaatgaa agttcacgat cagaaaagcc
    34441 ctcaattcta gtactgggat ctggtcctat ccgtatcgga caaggggttg aatttgacta
    34501 tgcgactgtg cattctgtca aagcaatcca gcaggcagga tacgaagcaa tcatcatgaa
    34561 cagcaatcct gaaacagttt cgactgactt ttctatttcg gataaattat attttgagcc
    34621 attgaccttc gaagatgtga tgaatgtgat cgagctagaa cagccgatag gagtaatcgt
    34681 tcaatttggt ggtcagacag caatcaactt agccgaacct ttgactgctg ctggtgtaaa
    34741 aatcttaggg acaacgatcg aagacttgga tcgtgcggaa aatcgtgatt tattcgagca
    34801 agcgctgaaa gagttggata ttcctcaacc tttaggggat actgctacaa gtaaagaaga
    34861 agccgtggtt atcgcttcga gaattggtta tccagttctt gttcgtccaa gctatgtatt
    34921 aggcggacgt gcgatggaaa tcgttgaaaa ccagcgagat ttagaagatt acatggaaca
    34981 tgctgtgaaa gcatctccag aacatccagt actagtggac cgttacttgc ttggtggtga
    35041 atgcgaagtg gatgcgattt gtgacggtga aacagtactc atcccaggga tcatggagca
    35101 tattgagcgt gctggtgtcc attcagggga ttcaatggct gtgtatccac ctcaaacatt
    35161 atctgaatcg atcaagaaaa cgatcgaaga ttacacgatc cgcttagcat taggtttgaa
    35221 ttgtgtcggg atgatgaata tccagtttgt catccatgaa aatgaggttt atgtgatcga
    35281 agtcaatcct cgagcaagtc ggactgttcc attcttaagt aaggtgacag gaattccaat
    35341 ggcacaaatt gcgacgaaag ctattttggg agagaaacta aagacacttg gttatgaaaa
    35401 cggactttat ccagaaacaa aagaagttca tgttaaagca cctgtctttt cttttacgaa
    35461 attgcagaaa gttgatactt acttagggcc tgaaatgaaa tcgactggag aagtaatggg
    35521 atctgacaga caattagaca aagctttgta caaggcattc gaagcttctg gattacactt
    35581 gcctgaatat ggagccgtat tatttacgat tgcggacgaa acaaaagaag aagcactaga
    35641 attagctaaa cgttttacag aaattggcta tagtctgata ggaacgaaac atacagctgc
    35701 ttatttcgaa aaaaatggtc tggtcgttga aacagtagcg aaaatttctg aggaagcagc
    35761 agaaaaaaat gtagtcgact taataagaga tggtaaaaca caagtggttg tcaatacgat
    35821 tgataaagat cggggaaatg cttctaaaga cggtttcatc atccgaagag aagcagttga
    35881 acatggtatt ccattgttta cttctttaga cacagcagat gcgatcgttc gagtgatgga
    35941 atcccgtgct ttttcaacta accctattta acggcgagca catgttaaga caaaaagacg
    36001 aaaaaccgtt gcaggagggg acagatgaaa caagaaatga tggaaatcgt tcagcaaaaa
    36061 cagctggcac caaagatttt tgaaatgaca ttgaaaggtg atctagtaaa agaaatgcag
    36121 caaccaggac agttcttgca tattctcgtg ccgcgtgcag acttgctatt acgccgaccg
    36181 attagcttga atcatatcga ccacgaaaca aatacttgcc gtatcattta tcgggtggaa
    36241 ggtgagggga ctagagtctt ctctactctt tctgccggag accatttaga tgtcatgggt
    36301 cctttaggca atggttttga tttgtccaat cttcatacag gagacgaagt atttataatc
    36361 ggtggaggaa tcggtgtccc tcctctatat gagttatcaa aacagttagt cgaaaaagga
    36421 attaagccaa cgcatttttt aggttttgcc acacatgaag tcgtttatta tgaaaaagaa
    36481 tttaatcgtc taggagaaac aagaatcgct actgacgatg gaacatacgg tatgcacgga
    36541 aatgtcggta atttattgtt aggaacgaag acggaaccta cagctgtttt tgcatgtggt
    36601 tcaaacgggt tattgaagac agtggaacag ctgttttctt cgcatcaaaa tgtccagtta
    36661 tcattagaat cccgcatggc atgtgggata ggggcttgct atgcttgcgt ttgtcatacg
    36721 ccggaagatg aaacaggaac aaaaagtgtg aaagtttgtg acgaaggacc gatttttaaa
    36781 ataggagagg taattatatg aatgagaatc ctttagcggt caaacttcct ggcctagaat
    36841 taaaaaatcc tatcatgccg gcaagcggct gtttcggttt tggcaaagaa tacggaaaat
    36901 actatgattt gaatcagctt ggatcgatca tggtcaaggc aacgactcca aatgcccgct
    36961 ttggcaatcc cacgccaaga gtggcggaaa caccatctgg catgttgaat gcaattggtt
    37021 tgcaaaatcc tggtctagac gttgttatgc ataaacttct tccagaatta gaggagtaca
    37081 atgagttacc catcatcgca aatgttgctg gtgcttgtga agaagactat gtagaagttt
    37141 gtagcaaaat tggcgaagca ccaaatgtga aagcaatcga gctgaacatt tcttgcccaa
    37201 atgtcaaaca tggcggaatt gcttttggaa cagattctga tgttgctttt caattaacac
    37261 aagcagtaaa gaaagtttct tctgttcctg tctatgtcaa attgtcccca aatgtaacag
    37321 acatcgttcc aatcgctcag gcaatcgaag ctggaggggc agatgggttt acgatgatca
    37381 atactcttct tggtatgcgg attgacctta agacaagaaa accgatttta gccaatcaaa
    37441 caggtggact ctccggacca gcaatcaagc ctgtcgctat tcgtttgatt caccaagtag
    37501 caagtatatc cagccttcca atcattggga tgggcggcgt gcagacagtg gatgatgttc
    37561 ttgagatgtt tatggctgga gcaagtgctg tagctatagg gaccgcgaat ttcaccgatc
    37621 cgtatatctg tcctaaacta attaaagagc tccctgtgcg aatgagcgaa ctaggcatcg
    37681 aatcgctgga aagattaatc aaagaagtga gagaggaacg tgaacgatga atcaactaga
    37741 ccaacgacca attattgcat tagatttttc aactaggcaa gaggtagaag actttcttcg
    37801 atttttcccg aaagaagaaa aattgtttgt gaaaatcgga atggaattgt tctaccaaga
    37861 aggacctgaa atcgtacgtt atctaaaaga ggctggtcat aatgtatttt tagatctgaa
    37921 acttcacgat attccgaata cggtcgaaaa agcaatgcgg ggattggcaa agcttggtgt
    37981 tgatctcacc tgtgttcatg ccgctggcgg tattcgtatg atggaggcgg caatgcgagg
    38041 attagaagag gggactccag aaggtggaaa acgtccgcta cttctagcta tcactcagct
    38101 tacttctaca agtgaagaag aaatgcatgc cgatcaacta atcgaagttt ctttagaaaa
    38161 aagtgtgatc cattatgcta gctgtgccaa aaaagcagga ttagatggtg tggtttcttc
    38221 tgcttgggaa gtagaagcga tcaaagaaat ggcaggagac gaatttgttt gcttaactcc
    38281 gggtataaga ccagaaggaa cagtcgcagg agatcagaca agagtagtca caccttctca
    38341 ggcaaaaaag atcggctcaa cttttatcgt tataggtcga ccaatcactc agtcaactaa
    38401 tccttataaa gcatatcaaa cgattcaaac agaatggagc taacctaaaa tgaatgttga
    38461 acaaagtatc gcaaaagact tattagaaat tgaagcagta tttttaaatc caagtaatcc
    38521 ttttacatgg gcaagtggaa tcaaaagccc aatttactgt gataaccgaa tcacgatgag
    38581 ctatccaaaa gtccgaaaag agatcgcaaa aggattagca agcaaaatca aagaagctta
    38641 ccctgaagta caagtgattg ctggaactgc aacagcagga atccctcatg ccgcatgggt
    38701 agcggaaata ctggatttgc caatggtcta tattcgaagt aaggcgaaag atcatgggaa
    38761 aggtaaccaa atcgaaggac gtatatttga aggtcagaag atggttgtga tcgaggattt
    38821 gatttctaca ggcggaagtg tcttggaagc agctgaagca gcaaaaagag aaggtgcgga
    38881 tatccttggt gttgcggcta tctttacgta tgagttgcca aaaggaaaag caaatttcga
    38941 aaaagccgaa atccctctta tgacgttgac caattattct gtattgattg aagcagcttt
    39001 agaagatcgt tatatcgatg agcaagaatt aactctgcta aaagaatgga aaaaagatcc
    39061 tgaaaattgg cagatttaag cgcgcttgat gaagatagag aaatccgatt tgtgtctcca
    39121 gaatcatcaa ttggaatagc aaaaatccaa aaactgattg cgagaagcat cttttcatca
    39181 ggtatgaaaa gaaacttcta tcgtgatcag tttttgattt ttttaaaatt catttttcta
    39241 agactggtta gcagaaagaa gagaataatc tcttctcttt tttctattat atatagattt
    39301 tctctatttt ttggtaaggg agctgtttca aaaatggtaa agagcggaaa tgttgcgaat
    39361 atcagttatt taatgatatg atattcggaa aaagaaatct caaagggagg taacttttat
    39421 ggggttgaat gaatttattt taggattgaa caatttagag acgatcacaa gggcacctgg
    39481 attttttaaa tatacagagc atacagttgc agcacattcg tatcgtgtag catctattgc
    39541 gcaagttcta ggagacattg aagaagcgaa tggacaaccg atcgattgga aactgctcta
    39601 tgaaaaatca ttgaatcatg actacacaga gcgttttatt ggtgatatca aaacgccggt
    39661 gaaatacgca agcaaagagt taagagggat gctgcaaaca gttgaagaaa agatgacgga
    39721 cgaattcatc aaccaagaga ttccagctga gttccaagaa gtttatcgcc gtcggttgtt
    39781 cgaaggaaaa gatgaaaccg tagaaggaga actattagcc atttcagata aggttgacct
    39841 tctttatgaa tcttttgatg aaatcatcaa aaataacccg gaaaaagttt ataaggagat
    39901 gttcattgag gcagtgacta cgattaaaag ctttgaacat cggccttctg ttcagtattt
    39961 ctttaaagag atatttccgg aactattgaa ccaggaattt tatggaaaag aggcgttttt
    40021 agaaaccgtc tcaacgattg taaagaaaga agaaaaataa aaatgatatg agatgcaggg
    40081 ggaagtatag ttgaaaaaga atatggatgt cgaatggggc tattctggag acaaaggtcc
    40141 ggaaaattgg gcgagtttat gtgattggtt tgcgagaggt gcggaatttc cattgcagtc
    40201 gcctattcat ttgaccaagg gggaagaaac caaaatagaa ggagacacgc tttcctttca
    40261 ttatcaaaag caacggttta ctgacaagga attcaaaaac acgattcatt tagtaccttt
    40321 tgaccaactc agttatgtag agttcaagaa agaacgctat tatttgacag atattcattt
    40381 ccacttgcct tctgaacata tcatcaacgg aaaacaggaa gatattgaat ttcattttgt
    40441 tcatatgaac agcaaaaaag agaatctagt tgtgggtgtc atgtaccagc taatggtaca
    40501 ggaaggttgg ctgtataatc aagaaaatgg ggaaagttgg aacttagaaa aacatgagca
    40561 ttgggtaaat ccggatgtgt tctttccgga gcaaaaaagc catttccatt atattggctc
    40621 attgacaact ccgcctacca gcgggccaat ccaatggttt gtaatggatc atgtaggaaa
    40681 actgaaccaa gagtttcttc tccccttcga tggtcagtat gctcgaccga acaaccgacc
    40741 aatccagccc ttgaatggaa gagaaattta ttatttcgag gagtttgagc aatgaatatt
    40801 cagcagatga aatacgtggt cgctgtagca aacaatggta gtttcagaga agcggcaaag
    40861 aaaatgtttg tctcccagcc aagtctttca gctggtatca aagaattaga aaccgagtta
    40921 gggatcacgc tgtttactcg aacgaatcga ggggcatatt taacagaaga agggcaagac
    40981 ttcttaaatc gtgccgagaa aatattggtc cagcttgata cattagaaac tcactatcta
    41041 acgagtgaaa agagagacaa tttttcgatc gctgcccagc attatgattt cttaggtcct
    41101 ttgaccgcac agctactcga ggaattcaaa gacgagatca aacaatttcg gatcatggag
    41161 acgacaacag cgagagtgat cgaagaagtc aaagagcagc acagtgagat cggcattatt
    41221 tatatgaatg agcataatca agctggtatc agaagatatt tacaacaagg agagctaagt
    41281 ggccgcagca ttggaaaatt caaaacgcat atttttttaa ggcaaggaca tccattagct
    41341 gaacacgcag agatcaccaa agaagaattg ttatcttatc ctcaagttcg ttttacacag
    41401 gatggaaata atttcccgta tttttatgaa gaccttatcg agatccctga tcaagaaaca
    41461 gtgatttata cgagtgatcg aggtactttg atgaatatcg ttcttgaaac tgatgcttac
    41521 gcttcaggat ctggtattgt gattggaggg attcgcgagc acttgcggtt aatccctttg
    41581 gcagaaggcg aactaaatga attttatatc atttattcgg cgaaacgtcc gctcagtgaa
    41641 atcgctcaaa gatttatcaa agagcttact cggttactgg accagcagag ctaagaaaag
    41701 cagatgagaa cgctgtttat tgaacaccgt tgatcaaaaa agaggggttg tgacagaatt
    41761 ttgtcacaac tcctcttttt gtagcaaaca atttataact aattgctgtt gtgggaaagt
    41821 acgcaaatat gattctagaa acactcattg attacctgtt ttgtttcata gctgtaattt
    41881 tttcttcttc tggtgtcacg ataatcgtct tttggttttc ataaataaca taaccaggct
    41941 tggcgccatt tggttttcgg atatgcttta cctgtacaag atcaaccggc acttgggcag
    42001 agtatcggta ttttgaaaaa taggcagcta actcggcagc ttctgtaatt gtttcatctg
    42061 agggcttgtc acttttaatg atgacgtgcg aaccgggtat attttttgca tgaagccaat
    42121 agtcggtttt tttagctgtc ttcattgtca gctggtcatt ttgaagattg tttttaccaa
    42181 caagaatgag cgttccgtca gaagaaaagt attgatcagg ctgagatggt ttcttacgtt
    42241 tttgtttttt taagttcttc tttttcaggt atccttctga agtcaattct tctttaattg
    42301 cttcgatatc cattgggccg gcaatttcca gttgagataa gacagattca agatattgga
    42361 tttcatcttt ggcttcttgg atttgttcgc caatcagctt aacagcgttc ttcagctttt
    42421 ggtatcgatg gaaatatttc tgtgcatttt gatttggtgt caatgccgga tctagtgcta
    42481 ttttgattgg ccgatcttct tcataataat taggtaaaac aacttctttt gcgccgcgtg
    42541 gtacttgtgt cagaaaagta gtaagtagtt cgccatttct acggtaattc tcagcgtttt
    42601 cggattcttt gagtgtttgt tcacgttttt tcaatttatt tttatttcgt tttagttcat
    42661 tttcgatttt tcgaatcagt tctccgcctt gttgttttgc ccgatctttc tctgcttttt
    42721 cgtgataata gctatctaat aattgactga gtgttggata agctgttaca gcagatgcct
    42781 gttcggacaa tacctgataa gcaatcggtg tgaaaaaatc tttattttcc gtttcgtaaa
    42841 aagtaggaat aggctgatgg ctgatggctg agaaaaattc gtcccataca accatctttt
    42901 cattaggacg ttccagtaat cttttggtca gctcttgagc gatatcgaaa ccgattccct
    42961 gaaactgacg ttggattgct tttggatcta gttcagtttg ggaaagccgc tgaaagattt
    43021 tttctttctc ggagctaaat gggttcaaga cctcttgttt tggcggagca acatattcta
    43081 caccaggaag caaagaacga taagtgtttt gagagcttcc aatgtgtttg atcgcatcca
    43141 gtatttttcc agtttctcga ttaactaaga cgatcgtact atgacgtccc attaattcaa
    43201 cgatcaatat gatattttgt agatcaccca actcgtttcg tttagcaaag tggaaatgga
    43261 tcactcgatc attttcgatt tgttcgatgt cttctaaaat agcgccatcc aaatattttc
    43321 gaagcatcat cacgaagtta ggcggattat caggattctg ataatcgatt tgggtaatct
    43381 gtacacgagc ataacttggg tgcgcagata ataataatcg atggtttttg ccgcgtgaac
    43441 gaatcaccag cacgatttca ttttcataag gctgatggat tttcgagatc cggccagata
    43501 gtaacgtttc acgcaattcg ttgaccatcg cgtgggtaaa tacaccatca aaggacatgg
    43561 ttcattcctc atttcttttc ataagtatat tcattataac gaaaaaaaga agaaaaacca
    43621 atttggccga atagtaaagc aaaagtctag aaaaatcttg gactaattac ataaaaaaca
    43681 gtgtttctat cgttgaaatc caaattttta ggt
//
LOCUS       DACDHL010000016        36950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDHL010000016 DACDHL010000000
VERSION     DACDHL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.016"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IV228_000899"
     CDS             299..1960
                     /locus_tag="IV228_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8716835.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IV228_000900"
     CDS             complement(2030..2377)
                     /locus_tag="IV228_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8716836.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IV228_000901"
     CDS             2461..3402
                     /locus_tag="IV228_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716837.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IV228_000902"
     CDS             3403..4446
                     /locus_tag="IV228_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716838.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IV228_000903"
     CDS             4452..5522
                     /locus_tag="IV228_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716839.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IV228_000904"
     CDS             5512..6465
                     /locus_tag="IV228_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8716840.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IV228_000905"
     CDS             6477..7487
                     /locus_tag="IV228_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8716841.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IV228_000906"
     CDS             complement(7772..8593)
                     /locus_tag="IV228_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8716842.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IV228_000907"
     CDS             complement(8690..8968)
                     /locus_tag="IV228_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716843.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IV228_000908"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IV228_000908"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8716844.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IV228_000909"
     CDS             complement(11724..12311)
                     /locus_tag="IV228_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716845.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IV228_000910"
     CDS             12486..13808
                     /locus_tag="IV228_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8716846.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IV228_000911"
     CDS             13963..14739
                     /locus_tag="IV228_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8716847.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IV228_000912"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IV228_000912"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8716848.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IV228_000913"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IV228_000913"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8716849.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IV228_000914"
     CDS             16629..17258
                     /locus_tag="IV228_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716850.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IV228_000915"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IV228_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8716851.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IV228_000916"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IV228_000916"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8716852.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IV228_000917"
     CDS             19445..19981
                     /locus_tag="IV228_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8716853.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IV228_000918"
     CDS             complement(20021..21784)
                     /locus_tag="IV228_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716854.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IV228_000919"
     CDS             complement(21787..23502)
                     /locus_tag="IV228_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8716855.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IV228_000920"
     CDS             complement(24281..24505)
                     /locus_tag="IV228_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716856.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IV228_000921"
     CDS             24705..27473
                     /locus_tag="IV228_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8716857.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27554..27973
                     /locus_tag="IV228_000922"
     CDS             27554..27973
                     /locus_tag="IV228_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8716858.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="IV228_000923"
     CDS             27985..28455
                     /locus_tag="IV228_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8716859.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="IV228_000924"
     CDS             28472..29251
                     /locus_tag="IV228_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8716860.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="IV228_000925"
     CDS             29238..30059
                     /locus_tag="IV228_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8716861.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="IV228_000926"
     CDS             30076..31125
                     /locus_tag="IV228_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8716862.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="IV228_000927"
     CDS             31138..32142
                     /locus_tag="IV228_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8716863.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="IV228_000928"
     CDS             32775..33794
                     /locus_tag="IV228_000928"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8716864.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33837..34718
                     /gene="galU"
                     /locus_tag="IV228_000929"
     CDS             33837..34718
                     /gene="galU"
                     /locus_tag="IV228_000929"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8716865.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35018..35395
                     /locus_tag="IV228_000930"
     CDS             35018..35395
                     /locus_tag="IV228_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716866.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36001..36615
                     /locus_tag="IV228_000931"
     CDS             36001..36615
                     /locus_tag="IV228_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8716867.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT        12087 a         6479 c         7562 g        10822 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gccacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttaggaggaa cagcaagtga
    33841 aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac acgttttttg ccggcaacaa
    33901 aagccatggc aaaagaaatg cttccaatcg tggataaacc aacgatccag tttattgtgg
    33961 aagaagcatt ggattcggga atcgaagaca tcttgatcgt aacgggaaaa gaaaaaagac
    34021 ccattgaaga tcactttgat gcaaatatcg aattggaaaa caatttgcgg gaaaaggaaa
    34081 aaacagaatt actcgcttta gttgaagaaa cgactcaagt caatcttcac ttcatccgtc
    34141 aatctcatcc aagaggactg ggggatgccg tgcttcaagc aaaagcattt ataggaaatg
    34201 aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga tgatatccca ttgactcaac
    34261 aattgatgat ggattatgaa gaaacacaag cttccgctgt tgccgtcatg cgtgtaccag
    34321 aaaatgaaac atctaaatac ggaattattg atcctaaaac agatagcgaa aagggtcgtt
    34381 gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa agcaccaagt aatttagcca
    34441 ttatcggacg ttatctactg actcctaaaa tatttgagat attagaaact caagaaccag
    34501 gagcaggaaa cgagatacag ctaacggatg cacttcagac tcttaaccag acagaggcgg
    34561 tctatgcgag agagtttaaa ggaaaaagat acgacgtagg cgataagtta ggatatatga
    34621 agacaaatat tgaatatgga cttcaacatt cggaaattgg gacttcatta agtgcatata
    34681 ttatccagtt gagtaaaaag ctaagaacag aaagttaact ggaaataagt ggcctgctga
    34741 gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg tcatttgcgc aactgtcgtt
    34801 ttagtttttt gactctagat cagttgagtc gtggaaaacg aaagatagaa aaagactttc
    34861 gtgaatagat tcagaaaata ttatgctaat ggtttaattt ttttttaagt tgtataaaag
    34921 aaaatgatta cataagagaa atgaaagcca agccagaaga ttcatagtag aataaaaagg
    34981 cgaatttttt aattataaaa aaaggtgtgt gttcactatg ggaaaattag tcgctgttgc
    35041 acagtcgctg cttgatatac tcaagtggct gatggaagga agcaattgga tatttgtgtt
    35101 gccactattg cttattagtc tatttatcat gtttcgcatg gtcagaaaaa aagaaacgtt
    35161 tctgattttt atctggtatg cagcatccgt tggggttgct accttgatgg ttgaattggc
    35221 gttaggtagt tgggaagctt gcgtatttgc tttatttttt gctgtactgt atctaatcat
    35281 ttatatccca tatatgaaaa gaaagtctcg gataggcaga aaaggtagac atacaactac
    35341 tttttctagt acagattctc tcgaacaaag tagaagaacg agaagaagac ggtaatattt
    35401 agtaggtata cgcaaaaaaa gataaaataa tggataaact attgagatga gagatttatt
    35461 taattggtgg aaaaaagctt ttttcactta tataaaagaa tcttttttaa aggaatgctt
    35521 gttgtatcgg gataagccat tccttttttt atgttattta tatatatata aatcatacca
    35581 atgttttttt gtattttctt gcttgcactt ggataagaga ttacttacaa taaattcaga
    35641 tagaaacagc tgaataaaaa aaggctccct gacccttagt tggcgctaag aatcagggaa
    35701 tgaccagtca cacacataca ccctgaccga tcattttgcc atagtcaatg cagtgcatcg
    35761 acctaattta ttgtacctaa tctagggtga aaattctacc cctatgtaga tattttccat
    35821 ggtctgcacg ttgttttatt tgctatgtca aggtattggg cgtattcgtt caatgccttt
    35881 tttgtgtttg aaacagcaaa gaccacatgt gttactgtct aactactgtt tttttatgta
    35941 cttccttaga ttaggcagct gtttctattc atgtcaagta tagctaggcg ggagagataa
    36001 atgagtttat gttattttga ctattatcac caattggaag agaaattaag gatagaagag
    36061 ccgctgatgc ttcaagctgg cacttggatc gcgaaacaaa ttaatcaagg tggtagattg
    36121 cagatatatg ctagccgcac actaagtggt gtagcatttg aattttggga tcaaaccccg
    36181 gatcttgtac caagtatatt gattgaaaat ccagccggag gtgtctatga aacgctagaa
    36241 ggtgcaggac aggcaattat tgatcagctt tctgtcaaat cagaagacat ttttcttctt
    36301 ctttcgaatg aaggaagaaa cccggcaatc atcgaactag ctcagtggat caaagaaaat
    36361 ggtcatccat taatcattgt tactggattt gatttgtcac gaagtatcaa atcgcaacat
    36421 agcagtggtt tgcgattatt tgaatttgct gatttggttt tggacaacca cgccaattta
    36481 catgatgcag tcttgacttt gccaaatcta gatccagcga tctgcggaac agcctctctt
    36541 gcaacgatct ttttgctgca gcaagttttg tattttacag caaaacagct agtccaggac
    36601 acaagtggaa aatagttatc cacatgttga caaagttcct taaaaaaata atgttccaca
    36661 taaaaatgga gcgtttgaaa aaaagaaaac gattattaat gaggattaac aacgactact
    36721 ttttttgttt tagttatttt aagatggatc atctttgatt ttaagcttgt tttgtttaaa
    36781 gtttgttatt tttataagtt gtttcacagt tatgagaaat gagttataag aatatgtagt
    36841 gtcagaaaaa taaaaagtag tggaaaaaga gacaagaaag aatccatatg gattctttct
    36901 tgtctcttaa tacacgttga tgaaagagtc ttccccaaaa ctctttacaa
//
LOCUS       DACDHL010000017        36575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDHL010000017 DACDHL010000000
VERSION     DACDHL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.017"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(239..1549)
                     /locus_tag="IV228_000932"
     CDS             complement(239..1549)
                     /locus_tag="IV228_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8716868.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(1731..2960)
                     /gene="rpsA"
                     /locus_tag="IV228_000933"
     CDS             complement(1731..2960)
                     /gene="rpsA"
                     /locus_tag="IV228_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8716869.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3095..3775)
                     /locus_tag="IV228_000934"
     CDS             complement(3095..3775)
                     /locus_tag="IV228_000934"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8716870.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(3824..4459)
                     /locus_tag="IV228_000935"
     CDS             complement(3824..4459)
                     /locus_tag="IV228_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8716871.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4525..5928)
                     /locus_tag="IV228_000936"
     CDS             complement(4525..5928)
                     /locus_tag="IV228_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8716872.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(5915..6946)
                     /locus_tag="IV228_000937"
     CDS             complement(5915..6946)
                     /locus_tag="IV228_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716873.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            6977..7198
                     /locus_tag="IV228_000938"
     CDS             6977..7198
                     /locus_tag="IV228_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8716874.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7363..8100)
                     /locus_tag="IV228_000939"
     CDS             complement(7363..8100)
                     /locus_tag="IV228_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8716875.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8253..9593)
                     /locus_tag="IV228_000940"
     CDS             complement(8253..9593)
                     /locus_tag="IV228_000940"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8716876.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(9830..11353)
                     /locus_tag="IV228_000941"
     CDS             complement(9830..11353)
                     /locus_tag="IV228_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8716877.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(11731..12342)
                     /locus_tag="IV228_000942"
     CDS             complement(11731..12342)
                     /locus_tag="IV228_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8716878.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12465..12590)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12672..13388)
                     /locus_tag="IV228_000943"
     CDS             complement(12672..13388)
                     /locus_tag="IV228_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8716879.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13394..13963)
                     /gene="scpB"
                     /locus_tag="IV228_000944"
     CDS             complement(13394..13963)
                     /gene="scpB"
                     /locus_tag="IV228_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8716880.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(13947..14744)
                     /locus_tag="IV228_000945"
     CDS             complement(13947..14744)
                     /locus_tag="IV228_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8716881.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(14714..15085)
                     /locus_tag="IV228_000946"
     CDS             complement(14714..15085)
                     /locus_tag="IV228_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8716882.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15104..15991)
                     /gene="xerD"
                     /locus_tag="IV228_000947"
     CDS             complement(15104..15991)
                     /gene="xerD"
                     /locus_tag="IV228_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8716883.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(16087..16560)
                     /locus_tag="IV228_000948"
     CDS             complement(16087..16560)
                     /locus_tag="IV228_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8716884.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(16665..17528)
                     /locus_tag="IV228_000949"
     CDS             complement(16665..17528)
                     /locus_tag="IV228_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8716885.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(17724..19823)
                     /locus_tag="IV228_000950"
     CDS             complement(17724..19823)
                     /locus_tag="IV228_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8716886.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(20043..21152)
                     /gene="rpoD"
                     /locus_tag="IV228_000951"
     CDS             complement(20043..21152)
                     /gene="rpoD"
                     /locus_tag="IV228_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8716887.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(21176..23017)
                     /locus_tag="IV228_000952"
     CDS             complement(21176..23017)
                     /locus_tag="IV228_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8716888.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(23199..25592)
                     /locus_tag="IV228_000953"
     CDS             complement(23199..25592)
                     /locus_tag="IV228_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8716889.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(25699..26559)
                     /locus_tag="IV228_000954"
     CDS             complement(25699..26559)
                     /locus_tag="IV228_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8716890.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(26618..27139)
                     /locus_tag="IV228_000955"
     CDS             complement(26618..27139)
                     /locus_tag="IV228_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8716891.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            27459..28178
                     /locus_tag="IV228_000956"
     CDS             27459..28178
                     /locus_tag="IV228_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8716892.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            28204..30795
                     /gene="mprF"
                     /locus_tag="IV228_000957"
     CDS             28204..30795
                     /gene="mprF"
                     /locus_tag="IV228_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8716893.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30983..32170)
                     /gene="tuf"
                     /locus_tag="IV228_000958"
     CDS             complement(30983..32170)
                     /gene="tuf"
                     /locus_tag="IV228_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8716894.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            32538..32894
                     /locus_tag="IV228_000959"
     CDS             32538..32894
                     /locus_tag="IV228_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8716895.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(33135..34139)
                     /locus_tag="IV228_000960"
     CDS             complement(33135..34139)
                     /locus_tag="IV228_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8716896.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            34305..35156
                     /locus_tag="IV228_000961"
     CDS             34305..35156
                     /locus_tag="IV228_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8716897.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            35173..35418
                     /locus_tag="IV228_000962"
     CDS             35173..35418
                     /locus_tag="IV228_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716898.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            35605..36309
                     /locus_tag="IV228_000963"
     CDS             35605..36309
                     /locus_tag="IV228_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716899.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        10332 a         7693 c         6300 g        12250 t
ORIGIN      
        1 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
       61 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      121 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      181 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      241 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      301 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      361 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      421 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      481 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      541 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
      601 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
      661 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
      721 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
      781 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
      841 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
      901 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
      961 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1021 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1081 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1141 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1201 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1261 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1321 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1381 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1441 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1501 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1561 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     1621 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     1681 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     1741 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     1801 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     1861 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     1921 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     1981 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2041 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2101 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2161 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2221 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2281 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2341 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2401 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2461 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2521 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     2581 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     2641 acttttttag catctaaacg gcgttttgaa agtagatagc tgccgttttc tttatctttg
     2701 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     2761 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     2821 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     2881 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     2941 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3001 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3061 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3121 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3181 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3241 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3301 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3361 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3421 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3481 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3541 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     3601 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     3661 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     3721 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     3781 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     3841 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     3901 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     3961 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4021 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4081 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4141 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4201 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4261 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4321 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4381 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4441 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4501 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4561 aatcgttccc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     4621 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     4681 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     4741 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     4801 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctc
     4861 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     4921 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     4981 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5041 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5101 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5161 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5221 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5281 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5341 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5401 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5461 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5521 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     5581 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     5641 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     5701 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     5761 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     5821 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     5881 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     5941 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6001 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6061 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6121 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6181 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6241 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6301 aaaggtggat acatctgctt tgttgcagat aaaccgctga ctcatcattg ttttttgcca
     6361 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6421 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6481 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6541 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     6601 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     6661 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     6721 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     6781 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     6841 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     6901 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     6961 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7021 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7081 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7141 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7201 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7261 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7321 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7381 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7441 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7501 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7561 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     7621 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     7681 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     7741 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     7801 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     7861 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     7921 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     7981 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8041 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8101 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8161 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8221 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8281 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8341 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8401 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8461 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8521 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     8581 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     8641 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     8701 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     8761 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     8821 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     8881 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     8941 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9001 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9061 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9121 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9181 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9241 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9301 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9361 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9421 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9481 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9541 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
     9601 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
     9661 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
     9721 gaaaaaacac tcactgaatg gagctattga cttatccacg tgcttatcga tagccgttta
     9781 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
     9841 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
     9901 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
     9961 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10021 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10081 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10141 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10201 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10261 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10321 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10381 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10441 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10501 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10561 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    10621 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    10681 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    10741 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    10801 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    10861 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    10921 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    10981 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11041 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11101 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11161 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11221 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11281 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11341 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11401 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11461 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11521 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    11581 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    11641 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    11701 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    11761 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    11821 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    11881 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    11941 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12001 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12061 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12121 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12181 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12241 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12301 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12361 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12421 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12481 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12541 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    12601 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    12661 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    12721 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    12781 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    12841 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    12901 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    12961 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13021 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13081 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13141 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13201 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13261 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13321 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13381 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13441 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13501 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13561 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    13621 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    13681 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    13741 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    13801 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    13861 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    13921 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    13981 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14041 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14101 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14161 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14221 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14281 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14341 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14401 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14461 tctgtgacga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14521 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    14581 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    14641 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    14701 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    14761 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    14821 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    14881 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    14941 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15001 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15061 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15121 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15181 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15241 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15301 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15361 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15421 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15481 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15541 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    15601 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    15661 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    15721 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    15781 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    15841 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    15901 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    15961 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16021 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16081 agacactcag acggactggt ttcttttttc tttttcatag cattcctgac agattccatg
    16141 gaacgttagc cgatgatctt ttactaaaaa atgataacgt tcttctacta cttgctccac
    16201 ttcaagtaaa agatcctctt ctacttcctc gatcgtgccg cagttcaagc atagtaaatg
    16261 atgatggaaa tgtttggcgc cttcttttct caaatcataa cgggctaatc catcattaaa
    16321 acttacttta tcgacaattt tcaattctgt caagatttca agggttcgat aaacagtagc
    16381 aagtccaatt tccggactct tttttttcac taaaaagaag atttcttctg cagataaatg
    16441 ctctttctca ttttccaaaa gcacaagcag agtagcttct ctttgcggtg tcagcttaaa
    16501 ccccgcttca tgtagctgct gttttgtttt tttcatcgcc gaaatagcat tcatgaccat
    16561 ttactcaacc ccaacaattt ataataatta caaataaaaa aagatataac actatgttaa
    16621 ttaaaattat tataagctag ttaagaaaaa catgcaaggg attctcaatt agattttttg
    16681 actagttttg tcacaccgtt ttcttgtgta atcaatcctt gtttcaaaag attacctaat
    16741 gcgcgcttaa atgctccttt gctgatacca aaagctttca tgatttcttc tggatccgat
    16801 ttatctgtaa agttgatttg ctgatctgca gaacgttcta aaatggttaa aatcatttgt
    16861 gcatcatctg atatagcttc atatgctctt ggcttcaatg aaagattcag tacaccgtct
    16921 ggtcggacac caatcactcg accttttatc tgctcgccta gacgaggttc attatatcgt
    16981 tcagaaggat ggataaaccc aagatagaaa tcttccgtta aaacgtacgt acctgttaat
    17041 ttcaagcggt atgctgttcc tatcacatct ttgtttttca tttcttctgt tccatatttg
    17101 cttaaagatt tgaatatttt ttcatctgct aaactgcccc aaatacggtc ttttgcatca
    17161 actctaaggg aaatcatcaa gcgatcccct tttttaggcc atagctctgt cattgtcggc
    17221 aattcatcaa gagatacgac aaagtcttta tctggaagac cgatatcgac aaacgcacct
    17281 aaatcttttc gtgtagcagt aacgattcca aaagcgtaat gaccgatacg gcttttagga
    17341 atttgtgtag tgatccgatt ttcatgtttt tgattttggt aaccgaatcc ttcaactgct
    17401 tcaccgattt tgtgttcacc ttctgatttg cttaagccaa aggtttgtcc gtttttttgg
    17461 acaaagtagt ttttttcatt ttcatcaata ataatacctg taaatatagt tgctaataaa
    17521 tcgttcattt ttttctccct ctatagtgaa aggttttctt aatacatttc tttgctatca
    17581 taacataatg ttatctactt gaatagctga gccttggttt ggaaaggtga aaaaaggagt
    17641 atgacaacag ttgtgtcata ctcccctttt tcgaataaac ggtgttaaac ggcaggctca
    17701 cagcctcttt catttgccta aaattaattg ctcccatttc ctccgaacca tccagtttta
    17761 cagttgatgg ttacgatgta aaatggcaaa gcactttctc caatgccttt catcaatgca
    17821 gattttgttg aagtggcata tagattataa tagccttccc cattttcgat tctggctttt
    17881 tctaatctgt aacctccttc aacgatatat ccacgttgga tacatgtagc aatcactttg
    17941 ctttggacac ctggtgccca cgtccatgct ggattagatg catcggcaat atcacttgct
    18001 ctggcagatt tgattggctg attcgctgag tttgaagagg aaggggtcga ggatgccgct
    18061 tcttgctgtg ctgcttgtgc gaccgcagct tgttgagcgg cttgagctgc agctgctgct
    18121 tgagcggctt gttttgcttg ttcttcagct gcttttttcg cttcttctgc ttttttcttt
    18181 tcctcttctt cttttttctc tttatcagtg agcatcttat ctacttgttt caattgttca
    18241 ttcaactgag cagatagatc tgtatttttc actttttcta ctgcttcttt tgccgtcttg
    18301 tattggtcgc gtgttgctga atccactact tttccgtctt taaagactat agctgttttt
    18361 tctttagctg tttgcaattc agcatattga tttttagctt ctgtttgtgc ttgttgaatc
    18421 aattttccaa aatctgtgtt atccgtttcg attgcttgga tctctttatc tgctttcaac
    18481 ttgatgtcat ctgctagatg atcatcgacg atggcaggtg ctatgaacca ctcattcact
    18541 ttttggatat ctcggatctt agattggata tcttggtaag ttgtttctaa ctctgaatat
    18601 cctttttcat tcttaagact ggataaatca ttttttagtt ttgataaatc ttgatgaatc
    18661 atatctgcac gaataaattg atggtgatca tctgtataaa aggcagcaag tttctcatga
    18721 agttcttcca tttgttgctg tctttttgct tctgctgcta cttcggcttc tactttttga
    18781 tgatggtcat aataagccca gcttcctcca ccgagtagaa gtactgctgc taaaacgaga
    18841 taaggtactt tttttgattt tttcttttgc gctttatctg gatctgtttt ttctaagttt
    18901 gattcttgat gtgttttttc agtatctggc acgattgtta ttgatttcgc ttctgcttca
    18961 aacagcttag gctggttctc ttctttttct atttttgctt caagatcagt accctctttg
    19021 cttacagatt gattttcagc atcggattca ctatttattt catcgggctc tgttgacttt
    19081 ttgtctgtgt tttcgagtat ttcttttttc tctgcttccg gcagtttatt cgttttttcc
    19141 ttttctaatt gtttttcagt aaatacttca gttttttctg tgatatccgt ttttacgtct
    19201 tttattttta ctgtttcttt acctgtggcg ttttctctat cagagtcttt ttcagtttct
    19261 tgttcctttt ctggctctac tttgttttcc tgtgcaattt tttcactgat cattttgagc
    19321 agctctttcg cgtgctcttc ttttgtatct tcacgatgtg ctttgatata agcggatagt
    19381 atcgaatttt cttcctcatt atcgacgatt tctttcagtt cagctactct tcttttttgt
    19441 tttgattcaa gcgtttcttc ttgattcttt cctgattctt ccgaagtctc atgaacagtt
    19501 gctttcttct gttcattttt ctctatatta gttacttgat cgtgagcagg tggttcttcg
    19561 tcttctggtt ctgctttttc gaataattcc attacagatt ctagaggtaa atctttcaat
    19621 tccgaccatt cgattggatc gtttaattct gtatcaggaa agactgcatc ttcagttgat
    19681 ttatcctgca tagctgcatc tgtatttgtt tcttcaattg attcttttat ttgctgatct
    19741 tctgcagtta tttgatgacc gcagtctgga cagatagtat tctgctccat ttttttcgat
    19801 ccgcaatttg gacatttttt catcacttag cgatcggctc ctttactagg tttacgctac
    19861 catttaatct atcatattct atacgtcacg ccaatagctt atttttagga tttacttaaa
    19921 aataatgaat gaatttcctt cctatagaaa aaaaagcttg agactgcgta gtctcaagct
    19981 ttttttcttt taaaataaac caaacagttt tactgatttc tcttctggtc aatagtccac
    20041 gcttattcta agaaatcttt taattgtttt gaacgagaag gatggcgcaa ttttcttaat
    20101 gctttagcct cgatctgacg aatacgttcc cgagtaacac caaatacttt ccctacttct
    20161 tccaatgttc tcgtccggcc atcatctaaa ccaaaacgta agcgaaggac attctcttcc
    20221 cgatcagtca atgtatctag tacatcttcc aattgttctt ttagtaactc ataagctgcg
    20281 tgttctgctg gacttgttgc ttcctggtct tctatgaaat cacctaagtg agagtcatct
    20341 tcttcaccga ttggcgtttc aagggaaaca ggttcttgcg cgattttcaa gatttctcgg
    20401 actttttctg ttggaagatc catttccgca ccgatttctt ctggcgtagg ttctcttcca
    20461 aggtcttgca gcaattgacg ttggatacgt atcaatttat taattgtttc aaccatatga
    20521 actggaatac ggatcgtacg tgcttggtca gcgattgctc gagtgatggc ttgacgaatc
    20581 caccatgtag cataggttga aaacttaaat cctttgcggt agtcaaattt ttcaactgcc
    20641 ttcatcagtc ccatgtttcc ttcttggatc aagtcaagga actgcatgcc tcgtcctaca
    20701 taacgtttag cgatacttac tactaaacga aggtttgctt ctgccagacg ctgtttcgct
    20761 tcttggtcgc cttcttcgat tttcaatgct aacgctactt cttcttccgc agttaaaagg
    20821 gagacacggc caatttcttt cagatacata cgaaccgggt cattgatttt tacaccggtt
    20881 ggcgcagaaa gatcttctgt ttttgcttgt tcagcttttt tctcagcact cttcaagctg
    20941 tgaattgatg gatcaccatt ttcatccacc acactgatac ctgcgtcttc aatcttttgg
    21001 atcaatttgt ccatttcatc tgcattcaaa gtgaatggtg tagcaagctg attggataaa
    21061 tcatcatata caacttgtcg tttgggtttg ttttctttga tgaaagctgc tactgctgct
    21121 tcatacgttt ttttatctgt tccatttgcc ataaaagaag gccccccttg agtagctatt
    21181 tggcttgttt taactgtttc gttaaattga tgatttcaat cgtcaattca agctcaagct
    21241 gtttgtttcc agttcgtctg gcttcttgct gcatttgttt ttttaatgtt atttcatcag
    21301 ctaagccaga tttgctgatc acatctagca agtcttggat ttctcgtggc gaactttctt
    21361 ctgccaccgg taaatattcg atttccacca ccaaccgcct taaattttca tcttgcaaaa
    21421 agtctaaaaa ctctgcaagg ttgaattcat aatgttgtga gacatatgca tcgaataaaa
    21481 aataaacttc tgcatatgca tcatgaataa actgaaattc tcgttctttt aaaatattac
    21541 ggacagaaac ttctttaaat gcacgatata atagaatctg ctctgccttt tctacttgcg
    21601 tcaaaggtct ctttttccgt gcagtttctt gtctcgtaac ctgagtgtgt tgactttcct
    21661 gaggttcttg acgattttgc tgatttgtcc ttcgaagatc gcgcagttgt ttttgtatcg
    21721 tctctctgcc gatctgaaac tctgttgaca gttgagatag atacatatct tgttcgattg
    21781 gtgaaacaac tcggctaagt tcaactagta attcttcgag gtattcgatc ttgtcttttt
    21841 cattatctaa atttttccct tgttttaaat aacgtgattt aaaagtaaag actgtttcgc
    21901 gcccatgagc cactaattct tggaaagatg cttctccata cttacggaga tattcatcag
    21961 gatcaagctt ttctggaacg actacgacag aaagtgctaa tcgactgttt tgccgaagca
    22021 attcaatccc tcgattggtt gcttcaaaac ctgcgttgtc tccgtcatag caaaagacca
    22081 attctttcgt cagcttttcc atttgttgga tctgttgagc tgttagactt gtccccatag
    22141 aagctaatcc agttttgata ccagcctgcc atgcagcaat cacatccata aacccttcga
    22201 acaacaaaat ttctccgttt ttccggatat cgcttcttgc tttatcaaga ttaaataaaa
    22261 cttgtctttt attgaacagt tctgtttctg gactattcaa gtatttgggc tgatcttttt
    22321 cctgcccttt ttcaggtgca agccagcgtc ctgaaaaacc gatcgtctgg ccttgagaat
    22381 tacgaagtgg aaacatcact ctttgataaa aacgatcaga catacggcca tcatcatgcg
    22441 tgacaaacaa tccagagcgt tctaacaatt catttgaaat tgattcattc ttgaatactt
    22501 gctgaagaaa agaacgctca ttaggtgcaa aacctatgtt gaattcttca attaattcta
    22561 aagacaaccc acgactttgc aaatattcta aagctggctg tccagctttt gtatgcaata
    22621 gcatgtgatg atagatttcc gctgcttttt catgagcagc aatcagtttc cccgtagcag
    22681 attcaggatt ttggacagct atagtacgct gacgccattg atcttctaac ggtatctgtt
    22741 cgaagtctgc cacttttacc actgcttctg gaaaacttag accttctagt tcttgaagaa
    22801 aagtaaacac gtttccgcct ttaccacaac caaagcaatg aaaaatctgc ttgtcttctg
    22861 caacagaaaa agacggtgta cgttcttcat gaaaaggaca cagccccaag taatttttac
    22921 cagacttttt caactggaca tattgtccaa tcacctcaac gatattcgtt tgactgcgta
    22981 ctgtttcgat aacctcttga ggaatccgtt gagccatttt ttcaccccct gctctattga
    23041 tatcttttgg gtttaacaga agatatttca ggagaataac cggtaaaaaa tcgcactaac
    23101 tgaaatcagt gcgattttaa aggacacaat tatacatgtt tgattgtatc atatttcatt
    23161 gctttttcaa gtataacaca tttctatctc attaagcatc aatttgctac tcttttcagt
    23221 aactgatttt gaatatcagc cttccagtac aatactcgaa gaacaatatt tacgagcatg
    23281 aatacaaaga agtatcctac gctctctccc aaccctaagt ttacttccaa aattccatgg
    23341 aacagcattg cagctacata tatccctacg atcgtcgtta ctgctaggaa gatgcgcaat
    23401 gggttgaagg gataacaagc ttttacaact gccatgcaac tgatcccaat cagcaagtaa
    23461 tacatcaaag tagtagtttc agcatttccc catgcgaatg tttttccaag tagatacacc
    23521 gcaatgatat tcactactac aagcaaggca tttggtaaag cattaataag tgctgtaggc
    23581 aaataacggt aagtgatttt tcttttatct tgttcaaaag ataaaaagaa ggaaggatac
    23641 ccttcaatcg ctaaatcaat cagcgtgatt tggataggga taaacggaaa tccaattgct
    23701 gtaacggcac atataatcga taaaataaat gaataaatcg ttttgataaa aaagatgcct
    23761 gagactttcg tcacattgtt gaccactcgg cgtccttcaa acaggacttc tggcaatgta
    23821 gtaaaatcag agtccaacaa taccagattc gcaatctgac gtgttgctcc atcaccttct
    23881 gccatagcaa tgctgcaatc tgcttctctt aacgccaaaa catcattcac accgtcgcct
    23941 gtcattgcaa ccgttctgcc gctttctttc aattcattta ccagcaattt cttttgctgc
    24001 ggactcactc gtccaaaaac cgtatagcga tgagcagctt ctcgcacttc catttcttca
    24061 gtaatttgag aaagatcgac atacgcatca taattcggta atcctgcacg tctagcgata
    24121 ttggagacag taaaaggatt gtctccagaa atcactttca gatccacacc ttcctcatga
    24181 agataagcta gtgtttcttg agcgtttttt cggattggat catcgatttc aaacagagca
    24241 accggctcaa caaatgctgg tttttcttcg ttcaattgct gatttggagc gatccccaac
    24301 ataagtacgc gatagccatt cgcttgggct gtttttagac tgtccggaag ccgatcttct
    24361 tccaccaaac gttcaggcgc tcctacgtac acgactccca gttctggaaa ttccatcgcg
    24421 ccccatttgc gatcagaaga aaatgcgact ttgtttgtca catcataccg attagaaagc
    24481 tcaaaataat ctctcatagc ccgcatagtg atgttgttat cactgctttc tgccagatac
    24541 gtccccataa tttctgggat ggctttctcg tacagcggat gtagtgcttc tacccgctgg
    24601 atactcattt tcccttcagt aatggtccct gttttatcca agcataatgt atcgacatgg
    24661 gccagcgttt caattgaata catgtcttga acaaggattc gtttttttgc cagtttggtt
    24721 acccctgtcg ttaatgcaat acttattagc aataccaaac cctttggaag catgcctaac
    24781 aaagctgctg cagatgcaat aacagaaatt ttgatagaag catcgcggat gaacagtgct
    24841 tccaaaaata gtatgatccc caaaggaata atcacgaaac tagtgaattt ggatactttg
    24901 cgaatcgaat ctacaagttc agaacgaata gctttgtgag tctttgcctc gctagtcaag
    24961 cggacagcat aattatctgc acctactcgg ataacttcag ccatgacaga accgcttgtc
    25021 aaaaagcttc cagaaagcaa ttcagctcca gtttctttga caatcaaatc agattcgcct
    25081 gtcaaaagag cttcatttgc ttctgcccgt ccagatataa cctgtaaatc ggctggtatc
    25141 tgttctcctg ctccgatttt taccaaatct cctaatacca gctctttagt agaaatcgat
    25201 tgttctttac cgtcccggat cactagtact tctgattggg aaacaatcga taatttccgt
    25261 acaagatttc ttgcatgtat ttcttgataa attccaataa cgatattgac gacaataatt
    25321 gctaaatacg ccatgttgga atacgcacct acaaataaca gacaaatccc aataccaaag
    25381 ttcagaaaat tgaataacgt caatatatta tcgctgaata tttgttttgt actttttgat
    25441 gcatcttcct ggtagtcatt ttgctggcca gaagccattt tctgaactac ctcttgctgt
    25501 gtaagtccat tcattttctt tttctccatt ttccgccaac tttcaaaaat aatcacgtgt
    25561 ttaagtctag cgtgttccaa gattaaaaac aacttgtttt tataaaattt atagaaaaac
    25621 acacgaatga ttttacaaaa aagatgccaa cctttttgtt tgaatcaccc gtgtgctgtt
    25681 tttattatca atctatcttt acttatattg ttcctccaaa cggctcatcc accaacctag
    25741 tgctgtaccg gctataaaaa gaacaggaca agctagtacc ccagcaactc ccaatcccgc
    25801 gtaatcggta ggaatgatca taatcgttga agcaaaaact acacctaaaa tgaaatggaa
    25861 tagcttgcta tatgcctttg agaaaatgta atccatgatt tttgacaatc caagaacaca
    25921 aagaatccca ccaattccga tcggcacaat cacttcaagt cgtacttctt taaatccgtc
    25981 agccatcgct ttatacaatc ccatatacac caaaaaatta gaagggctca atcctgggac
    26041 gatcataccc aaaccaatca atccgccggc aatcagccat gttccaaaat tctgatcgac
    26101 ttgcccgtca aataattgag cgccgtataa taaaaacaaa taagccagaa caaaagtaac
    26161 aaccatgatg atcacatcag aagtatctct tccttttttc cctgcttctt tccataacgc
    26221 aggaacagtt ccagcgatac atccgacaaa aaaccataat atcgttgttt catacgcacc
    26281 taataaaaaa ctgactaaaa aagaaagtag aaagatgcct gtcactccgc ctagaccaac
    26341 aggcagaaaa aacaaaacat tttctttgaa attttttgta aggtgggcta aaaaagaaat
    26401 cagccgctca tacatgccaa agacagctgc taatgcacca ccgctgactc ctggcaagat
    26461 aaatcctgaa ccgataaaca ttcccttcac aaaccgtaac atccagtctt tggttgtgta
    26521 ccccacttct tgattttcca ttttttgcct tttttccact atttgcgtac tccttctttc
    26581 atttaatttt attcatgatt gaacatttcc tgctatttta cttaaagact ttttcatagg
    26641 tctctattat tttttcattc attttgacaa tatcaggata aaacatgtta cctccattat
    26701 gatagagata gccgccattg taaagcaagg actggatccg ccaatatcgg tattgtttcc
    26761 cttcctcatt tcctaaaagt gggctcagca catctttaga atacgcttct gctaattcta
    26821 ctgtgttttc tccgccatga ttttttacat aggcaatata atctttccca aaattatatg
    26881 cttggacagc tgtccataaa tcgcatcctt gtttctttgc ttctactatc atttccgcca
    26941 aatatttgac accttgatca atactgtcag ctggattatc aatgatctca gcttctccat
    27001 gcagactttc cgagctctgc atgagatctg tcccttttcc ttttgattcg gtcaatatta
    27061 tcgccttgat caaattctcg tagttttcca tatgataatt ttttgcagct gttgttattt
    27121 ctatttgata gctttccact cgttttaccg aacgataagt tcttcctcat aaaaacaaca
    27181 ccgcaccaat aaccgtcaga atgatcatgt aaaaaagaat acggataata gataatcgtt
    27241 ttcgcttcat ttcatcacct agttattttg acatcttttt ttatcttact atttctttat
    27301 cttcttggct atttcttcct tgatttctgt gtacgcctta agaaaaaatg aattttcctg
    27361 tattttttga catcttacga aaatgatcgt tgtttttcag taaaaacaat tgcccaatga
    27421 ctgatataat cgttatactt tacacagtag gtgattatgt gaaaaaagaa aagaaatatg
    27481 caaaaatgcc agatgggagc gaaatttact atgaaaagag cggtcaaggc ttccctctct
    27541 ttttactgca tggcaatgac ggcagcggcc gctttttttc agaacaggtc cctgtactcg
    27601 aacgctacta taccgtgtat ctagtagata gccgaggaca tggtcgttcg acaaacgaag
    27661 catctatgtt gaattttcag ttaatggccg aggatctgaa tacgatcatg ttgctggaaa
    27721 aaatcgatca ggcagatttt cttggattta gtgatggagc aaatctagca ctggtatttg
    27781 ccagcagctt ccctaaaaaa gttcatcgat tgattttaaa ttctggaaac acgcttgtta
    27841 aaggcgttcg tttttctgcc agagtcatca gtaatattca ttatgcatgg gtatggttac
    27901 tctctttatt tcgcccaagt cttcgcaaaa atcttttagt tatcaaactt ttgctgcatg
    27961 acatcgggct tacagaaaat gatttaaaaa aaatcaactc ccccactttg atcatcgtgg
    28021 gaaaaaaaga tgtaatcaag ctaaaacatt ctttgtatat agctaaaact ataccaaaag
    28081 cttcctttgt gcttgtgaaa gaacaaggac atgaattagc tagaaaagat cctgaacgct
    28141 tcaatcgaga agttcttcaa tttctttctg aaacataact attaggaata gaaggtgatt
    28201 tatttgttaa aaaataccat acaatggctg aaaaaccacc taggactgtt caaaacaatc
    28261 tttttgattt ctgtcattgt cattattgtc ggtgaattga tgtccatcgg taaaactcta
    28321 tctatccagc aattaggaga aacgtttgcc actatacctg tatggaaaag cggactaatg
    28381 ctattgattg gtcttatctc tgtgttacct atggttggtt atgatattat tttgaatcgg
    28441 atgcttgaac aaaagcagaa tcctcgttac ctgtttgaga caagctggct gatcaatacg
    28501 atcaacaata ttgccggttt cggcgggttt gttagtgtgg ggcttcgctc tgaactatat
    28561 ggacaaaaga aagagagcag aaacgtgatc caagcattat cgaaagtttt cttgtttttg
    28621 atggctggac tttctattta cagtctgctt tcttttttac ttgtagtctt cacgccagtt
    28681 gctccttttt tgaagcaata ttggatatgg ttgatcggcg gcggtctata ttttcctgtc
    28741 gtctttttat ttactacttt caaaaagaaa ggattccttg gtggtacctc acttaaaact
    28801 caaggacagc tattgctagt ttcattgttg gaatggaccg gtgtcctgtt tagtttcctt
    28861 tctgttggtt atttaatgga aatccggatt gatctgtggc aaacaattcc attattcatt
    28921 gctgcttcag tgataggaat cgtgtctatg attccaggag aaattgggag tttcgatgta
    28981 atgatgatta tcgggttgtc cgctattggt gtgccaagag aaatagtcgt tgtgtggatc
    29041 ttgctctatc gcctgttcta ctacatcatt ccgtttttga ttggaatcgt ctttttcttc
    29101 aaaaatatcg gttccacttt tgatcagcgt tattctggta tccctaaaca attagcaaca
    29161 gaaattgcgc ataaaatcgt ggttgtctta ttatactttt ctggaatcat gctagtactt
    29221 tctgccacta tcccgcaagc ttttacagag tttcgctggc ttcatagttt gaacccattg
    29281 aaatttcatt ttattatcca atttcctagt atactattgg gatttctttt aatcattatg
    29341 ggcagaggga tcgcagcacg tgtgaaacga gcatatttgc ctacaatctt cttgattgtt
    29401 ctagctttac tttatgtttt attaagtgat tttagtttca caccggtggt tttcttgtct
    29461 attctgcttc tgatcattct tgcttctaaa aacgaattat ttcgggaaca attgatctat
    29521 tcatgggagt ggcgtacgat cgatggaatc attattggtg cactgtcctt gctttacatc
    29581 gttattgggg tatacaatct gccggatttc cctcatcggc gtcaccattt tatttccttt
    29641 ttcttatttc cttctgaaaa gatttggttt tcgggattgt tggccatcat cgctgttagt
    29701 ttcatgatcg ttctatttgt tcatttcctt caaggagaaa agaaacagat tggcgaagcc
    29761 tttaatgaag aaaaggccct gaagatcctt acaacttatg gaggaaactc cgatagtcaa
    29821 ttgatcttct taaaagataa gcggatgttt gcctatgaaa aagacggtga accaactgtt
    29881 cttctccaat tcgcttgctt caataataaa tgtattgtaa tgggtgatcc ttctggtaag
    29941 aaagaagatt ttccagaagc aatcgaagct tttatcgaag aaacagatcg actatgctat
    30001 ttaccagttt tctacgaaac aagcgaagag attgttatga ttttgcatga attcggctac
    30061 gatttcatta agatggggga agaagcttat gtagacctga atagttttac aacttcaggg
    30121 aaaaaaatga agggcactcg tgctgtcttg aatcggatcg aacgagaagg atttacattt
    30181 gatgtcttac aaccaccatt ttctactgag caaatgagta ctttcaaaaa tatctctgat
    30241 aattggttgg gttcacgaaa ggaaaaaggg ttttctttag gctttttctc tgaagattat
    30301 ctacaaagag cgcctattgc agttgtcaaa aatacccaag aagaaattgt cgcttttgca
    30361 acgatcatgc ctacttacac gaacaataaa gtaggaacga ttgatctgat gcgatatgat
    30421 tctaaaaaag caccttctgg cagtatggat ttcttgttct tgcatttgtt tgcctatatg
    30481 aaagaagaac atatccaatg gttcaattta gggatggccc ctctttcaaa tgtcggaact
    30541 tccagaaaga gttttttaca ggaaaggatt gcatacttgg tttacgagtt tggttctcat
    30601 ttttattctt tccatggatt aagagaatat aaaagcaagt acgctacaaa ctgggtctca
    30661 cgttatactc tatattctag agatagttgg attgcatatg ttatgattgc tttgctgatt
    30721 attgataatg cgccagttga gcagacacaa ggtagaataa gtggtttgaa aaaatggata
    30781 cgaagaaagt attaaaaact gcataccgat agatgaacac tctcatgatg aataaaagag
    30841 actgaagtaa aacgaaagtt catgcttcta caatctcttc ttatacaatt gaagaacaaa
    30901 gagcggtcca aacagaaatc ctgtttggac cgctctttta ttttaaacat ttttcattac
    30961 caaaatattc gagtcgcgtt tcttatgctt cgatctctgt aacgatacct gaacctactg
    31021 tccgtccgcc ttcacgaatt gagaatgtcg ttccgttttc aacagcgatt ggatggatca
    31081 agtcaacatc aatcgtcacg ttgtcaccag gcatgaccat ttctgtatct tctggcaatg
    31141 tgattgttcc tgtaacatca gttgtgcgga agtagaactg tggacgatag ttgttgaaga
    31201 atggagtatg acgtccacct tcttcttttg tcaacacgta cacttctgct ttgaattttg
    31261 tatgtggagt aatggaacct ggtttagcaa gtacttgtcc acgttcgata tcgtcacgtt
    31321 ggatcccccg taacaacacg cctacgttat ccccagcttc cccgtaatct aacgttttac
    31381 ggaacatttc tacacctgtt acaactgctt tttgtgtttc aggtttgatc cctacgatct
    31441 ctacctcatc accgacacga acagcaccac gatcaatacg acctgaggca acagttcctc
    31501 gacctgtaat tgagaagacg tcttccactg gcaatagtaa tggtttatct gtatcacgtt
    31561 ctggtgttgg gatatattca tctactgtat ccatcaattc cataatagca gcttcagcat
    31621 ctggatcgcc ttgcaatgct tttaatgcgg aacctttgat cacaggagtg tcatcgcctg
    31681 ggaaaccata ttcgctcaat aactcgcgaa cttccatttc taccaaatcg atcaattctt
    31741 catcatcgac taaatcaact ttgttcaaga aaacaatcag gtattttaca ccaacttggc
    31801 gtgacaataa aatgtgttca cgtgtttgag gcatcggacc gtcagttgca gaaacaacta
    31861 agattgcacc atccatttgt gctgcccctg taatcatgtt tttcacatag tccgcgtgtc
    31921 ctggtgcatc aatatgcgcg tagtggcgtt tttctgtttc atattctacg tgtgcggtat
    31981 tgatcgtaat cccacgttca cgttcttctg gagctgcatc aatactagca tagtcttgag
    32041 gatttgctag gccttttttg cctaatactg tagtgatcgc agcagttaaa gtagttttcc
    32101 catggtcaac gtgtccaatc gtaccaatgt taacgtgtgg tttacttctg tcataatgtt
    32161 cttttgccat aatttacaaa cctcctaaat aatatagatc actgaaaaat ttcagctctc
    32221 acctttttat tatagtcttt ttttgactaa atcaaaaaga atacgtccga cttacttttt
    32281 gtaagattcg aactaagctt attatacgtc aatattggtc aaagtcaact aagagcactc
    32341 gtctgtctta tacgcgaaaa aagtaccttt tataaataaa aaaatgtgtt ttttgagaaa
    32401 aacttcaaga tagtccttaa ctttcttttg aagcaataag aacaaaatga ggaaaagttt
    32461 aaaattcagc tgttgccaac aactttcaat agacgaaatc ttctatttcg ttaataataa
    32521 ggaaagaggt gaagtacatg aaaaaattaa ttggcgtact tgtcgtttta ggcatatttc
    32581 tcggcggaag ttttgcagct tatcattatt tttacggcgg cgaagcttat tatacaaaaa
    32641 tcacgacgaa tggcgaaatg tcagctaccc aagcagataa cggagagaaa ttcacgactt
    32701 atacctacaa gcaagcggct tataataaag atggtgaaaa aaaacaggtg acgcttcgcg
    32761 aagaaagaga acatccatta aagatgaatg cgtatcttaa actcaaagtc aatcctcgta
    32821 aaggtgtatt aagctgggaa gaaataaagg cttctgaagt gcctaaaaat gcggcagaaa
    32881 aaatcgataa ttagtaaatg gtcttaaaat tttattagat aaaaagaggc tgggacagaa
    32941 gcgtttaact ccaagaaata agaaggagtt gcttctgctt ccgccgttta tacgtgttta
    33001 gagcgtgaga caaaagtgtt ctttactttt gtctcacgct ctttttttgt tatattcgtc
    33061 ttttccgatc cttctcttga cacaattcat ttaactgatc actggtactt gaacacctaa
    33121 tattctagtc atttttataa acgattcaat acttcatcca catttttgat catcacggat
    33181 atcgtaccat ccggattatt cacaaattca atcaaatctg ggttgcgata tacatctact
    33241 ggtacgatca gttcgatccc gtttgatagc ttgaattttt gttttccaaa ttttttctca
    33301 gaaatttctt gtacttcttt tacaggaggt gcttctggga caaatccttg ctcttgtact
    33361 tcttctttaa aagccatttt tgccgtgatg ttttccttga agactttttc cgcaatcatc
    33421 tcatgatcca accgaccttt ttcttctatc gtatcataga cagcttcttt caaatcagcc
    33481 atgatatcaa attcttctgt ctcaaatttt tttccaagat gcttggctac tttttggatc
    33541 acttttacat tttcttctaa agaaggtgca ggaacacttt cgatcactct tccagaaaaa
    33601 taaagctctt ttttgccaga aaactcatat tttttttcaa ttaattcgta ggtccaatca
    33661 gtcaaattca cagcgaatgc ttcatctgct ttttgtgttt tcgcccctag aattgctcga
    33721 ttcaaaatca agcggttgtc gatccctgct tcgtctgctt caacaaagtg agtgtaagcc
    33781 tcacgataat tgactttcaa taaagataag tacatcactg tatctaattc atacaagaca
    33841 acaaatacat ctgcactagg tgcttcttca ctttcttggt agatatcgaa ccaccgctga
    33901 acgattgctt cacttgcttc cacaaaattt tcttgagcga ttttaagtga ttcaacgatc
    33961 atgctttctt ctgctagttg tccagttttt gtttgagcag aagagatttt ttggatcttc
    34021 ttttgaagaa aatcacgaat atattctttt gtcagatcca gctctttttg tgaaaagact
    34081 ggagaaccag tttctctgtc aataatatgc agaattgctt tttttaaata gatatccatt
    34141 ttctcattcc cttctgtctt agtttacgga aaaaaaatca aaaataccag aatcaaaatc
    34201 attttctttt aaacaaaaat ttcaagagta gaaacaaaat ggattctttt tttcgaaaaa
    34261 tgcgctacac ttatagttga acgaataaaa aaggagctga aagtatgaat tcattaacaa
    34321 gcacttatcg tctatcaaat ggttacgaaa tcccggtagt aggattcggc acatggcaaa
    34381 cacctgatgg ggatgtcgct gtttcctctg ttaaagaagc gctggctgca ggttatcgcc
    34441 atatcgatac tgcccaagga tacaagaatg aagaaagcgt cggccaagcg attaaggaaa
    34501 gtggaattcc tagagaagag atattcttga caacaaaatt atggaatgca aaccacagct
    34561 atgaacttgt gatgtcttct tttgaagaat cattgaagaa gctccaaaca gattatttag
    34621 atctgttttt gatccactgg ccaaaccctg ttgcttttcg tgataattgg gaacaagcaa
    34681 atgcagatac atggcgagca tttgaagaac tgtatgaagc aggtaaaatc aaagcaatcg
    34741 gtgtgagcaa tttcttaccc caccatctag atacactagc aaaaacagca aaaattatgc
    34801 cgatggtcaa tcaagttttc cttgcaccag gcgaattaca gacaactgta gttgagtatg
    34861 caaaaaaaca tgatatgatc ctcgaagcat atagtccatt aggcactggt aaaatcttcg
    34921 atgtgcctga aatgaaacaa atcgctgaag cacatgacaa gacgattgca caagttgctt
    34981 tgcgttggtc gctgcaacac gaattccttc ctcttcctaa gtcagtgaca ccaagcagaa
    35041 tcaaagaaaa tactgaatta tttgattttg aattaacaga agaagaaatg aaacaaatcg
    35101 atcagttaga tggtgtagtc ggcaaagcaa aagacccaga tactacccaa ttttaagtcg
    35161 aaaggtgatt ttatggatcc aatcgaacgc ttgaattctc tctcggaaga agttacccag
    35221 acttttcatt ccgattttgt ctttttgatc gatgcagaga aaatccaaca ttttcctgca
    35281 agaaactgga cacatgatca aatcatcgaa gaactgaaaa aacggttcga tcattcgcta
    35341 atggtcaaac cctggcacga acatgaagtc atttattcac ctgaactacc tgtttttgcc
    35401 ttgataccga aaaaatagaa agaatggaac ggattatgta tatatacata atccgttcca
    35461 ttttttattt ttctttcaaa aaacgggaaa atacgcaaat ttttctgttg taatttgcgt
    35521 atacgcaagt ttttttgcat tactcctttt catttggctg tttttttctt ataatcgaaa
    35581 gggtatgagc gaaggagaga acatatgaag aaaaaacaga aacaaaaatt attaaatcaa
    35641 ttccgtcctt cattggatgg tgtaagagga caattattcc gttctctaga agaagagagc
    35701 ttgcgacgtt atggtttgcc gttacaagtc atgcttgatc ccaaaaaccg tcaaatctta
    35761 acgatcggat tagctgggaa aacaaatgaa gatacacgga tcaacgtgcc aatcgatgac
    35821 aacttcaata ctgtattaaa gagaattcgt tcaggagaaa acgggatttt cgaacgattc
    35881 cgtgacaatc ttcttattga aatcgtgtct tactggaatg atcaacgatt gaaaaacgat
    35941 gaaccaactg cgcaagctgt tgcgacacca gttacagaag aaacagtcag tgtgccagaa
    36001 gaaaaaacgg aaaaaccaaa agcggacaca actgaaaata cacgctcaac tgatacgaat
    36061 gccgctttaa gttttgctga tttttcaaaa gccgttgctg agtatcctaa attttacaca
    36121 gaacaaaatg aacatgaagt agttattaat gaaaaaaatg ctgatgaacc tcgtaaatta
    36181 gcaactattt ctatgactac tgaaaatgag ttcgttatcg aaaaagcact cgaacggaaa
    36241 tacaaagtca aattgacttt gattcctttg atcgaacaat ttgcagcgac agcgattact
    36301 gatcgataaa aattctattt gtcataaaag gctgggattt aaaatcccag cctttttagt
    36361 catataataa tttctacagc aagtaaaaag aaattccttc taacatcagg ctcaaatatc
    36421 agcttgacct gtccaacaaa tcacctatga tcctacaatg ttaggcgact tctcttctac
    36481 ctgaagatat cttggagttg cctgatttct taagatgcct aaccttaact ataaaaaatt
    36541 ctaataataa acatattttc tgatagagaa aaaag
//
LOCUS       DACDHL010000018        36569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDHL010000018 DACDHL010000000
VERSION     DACDHL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.018"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IV228_000964"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IV228_000964"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8716900.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IV228_000965"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IV228_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8716901.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IV228_000966"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IV228_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8716902.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IV228_000967"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IV228_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8716903.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IV228_000968"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IV228_000968"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8716904.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IV228_000969"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IV228_000969"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8716905.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IV228_000970"
     CDS             complement(8054..8524)
                     /locus_tag="IV228_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8716906.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IV228_000971"
     CDS             complement(8570..8995)
                     /locus_tag="IV228_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8716907.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IV228_000972"
     CDS             complement(9062..9331)
                     /locus_tag="IV228_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8716908.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IV228_000973"
     CDS             complement(9342..10937)
                     /locus_tag="IV228_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8716909.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IV228_000974"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IV228_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8716910.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IV228_000975"
     CDS             complement(14490..15050)
                     /locus_tag="IV228_000975"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8716911.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IV228_000976"
     CDS             15403..16377
                     /locus_tag="IV228_000976"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8716912.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IV228_000977"
     CDS             16547..17947
                     /locus_tag="IV228_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8716913.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IV228_000978"
     CDS             complement(18025..18747)
                     /locus_tag="IV228_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8716914.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IV228_000979"
     CDS             complement(18826..19332)
                     /locus_tag="IV228_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8716915.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IV228_000980"
     CDS             19455..21254
                     /locus_tag="IV228_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8716916.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IV228_000981"
     CDS             complement(21322..22896)
                     /locus_tag="IV228_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716917.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IV228_000982"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IV228_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8716918.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IV228_000983"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IV228_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8716919.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IV228_000984"
     CDS             complement(24673..25311)
                     /locus_tag="IV228_000984"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8716920.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IV228_000985"
     CDS             complement(25312..25962)
                     /locus_tag="IV228_000985"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8716921.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IV228_000986"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IV228_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8716922.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IV228_000987"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IV228_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8716923.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IV228_000988"
     CDS             complement(29105..29845)
                     /locus_tag="IV228_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8716924.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IV228_000989"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IV228_000989"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8716925.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IV228_000990"
     CDS             complement(31216..32154)
                     /locus_tag="IV228_000990"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8716926.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IV228_000991"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IV228_000991"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8716927.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IV228_000992"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IV228_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8716928.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IV228_000993"
     CDS             complement(35314..35619)
                     /locus_tag="IV228_000993"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8716929.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IV228_000994"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IV228_000994"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8716930.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6706 g        11989 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaat
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaata
//
LOCUS       DACDHL010000019        36347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDHL010000019 DACDHL010000000
VERSION     DACDHL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.019"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..695
                     /locus_tag="IV228_000995"
     CDS             24..695
                     /locus_tag="IV228_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8716931.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="IV228_000996"
     CDS             754..1194
                     /locus_tag="IV228_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716932.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="IV228_000997"
     CDS             1206..1874
                     /locus_tag="IV228_000997"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8716933.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="IV228_000998"
     CDS             1938..2561
                     /locus_tag="IV228_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8716934.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="IV228_000999"
     CDS             2617..3003
                     /locus_tag="IV228_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8716935.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="IV228_001000"
     CDS             3216..4607
                     /locus_tag="IV228_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8716936.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5218..5586
                     /locus_tag="IV228_001001"
     CDS             5218..5586
                     /locus_tag="IV228_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8716937.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5766..6707
                     /locus_tag="IV228_001002"
     CDS             5766..6707
                     /locus_tag="IV228_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8716938.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6776..7507
                     /locus_tag="IV228_001003"
     CDS             6776..7507
                     /locus_tag="IV228_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8716939.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7651..9831
                     /locus_tag="IV228_001004"
     CDS             7651..9831
                     /locus_tag="IV228_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8716940.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9851..10333
                     /locus_tag="IV228_001005"
     CDS             9851..10333
                     /locus_tag="IV228_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8716941.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10349..11110
                     /locus_tag="IV228_001006"
     CDS             10349..11110
                     /locus_tag="IV228_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8716942.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11241..12575
                     /locus_tag="IV228_001007"
     CDS             11241..12575
                     /locus_tag="IV228_001007"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8716943.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12882..13367
                     /locus_tag="IV228_001008"
     CDS             12882..13367
                     /locus_tag="IV228_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8716944.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13360..14043
                     /locus_tag="IV228_001009"
     CDS             13360..14043
                     /locus_tag="IV228_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8716945.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14169..16814
                     /gene="polA"
                     /locus_tag="IV228_001010"
     CDS             14169..16814
                     /gene="polA"
                     /locus_tag="IV228_001010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8716946.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16859..17695
                     /gene="mutM"
                     /locus_tag="IV228_001011"
     CDS             16859..17695
                     /gene="mutM"
                     /locus_tag="IV228_001011"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8716947.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17659..18288
                     /locus_tag="IV228_001012"
     CDS             17659..18288
                     /locus_tag="IV228_001012"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8716948.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18340..18672
                     /locus_tag="IV228_001013"
     CDS             18340..18672
                     /locus_tag="IV228_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8716949.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18984..19475
                     /gene="nrdR"
                     /locus_tag="IV228_001014"
     CDS             18984..19475
                     /gene="nrdR"
                     /locus_tag="IV228_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8716950.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19498..20871
                     /locus_tag="IV228_001015"
     CDS             19498..20871
                     /locus_tag="IV228_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8716951.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20871..21797
                     /gene="dnaI"
                     /locus_tag="IV228_001016"
     CDS             20871..21797
                     /gene="dnaI"
                     /locus_tag="IV228_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8716952.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21926..22366
                     /locus_tag="IV228_001017"
     CDS             21926..22366
                     /locus_tag="IV228_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8716953.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22510..23211
                     /locus_tag="IV228_001018"
     CDS             22510..23211
                     /locus_tag="IV228_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8716954.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23208..24329
                     /locus_tag="IV228_001019"
     CDS             23208..24329
                     /locus_tag="IV228_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8716955.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFLPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24344..25576
                     /gene="pepT"
                     /locus_tag="IV228_001020"
     CDS             24344..25576
                     /gene="pepT"
                     /locus_tag="IV228_001020"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8716956.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25708..26616
                     /locus_tag="IV228_001021"
     CDS             25708..26616
                     /locus_tag="IV228_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8716957.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26651..26923
                     /locus_tag="IV228_001022"
     CDS             26651..26923
                     /locus_tag="IV228_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8716958.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26934..27980
                     /locus_tag="IV228_001023"
     CDS             26934..27980
                     /locus_tag="IV228_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8716959.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28231..30840
                     /gene="clpB"
                     /locus_tag="IV228_001024"
     CDS             28231..30840
                     /gene="clpB"
                     /locus_tag="IV228_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8716960.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30991..31662)
                     /locus_tag="IV228_001025"
     CDS             complement(30991..31662)
                     /locus_tag="IV228_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716961.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(31655..32149)
                     /locus_tag="IV228_001026"
     CDS             complement(31655..32149)
                     /locus_tag="IV228_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8716962.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32169..33389)
                     /locus_tag="IV228_001027"
     CDS             complement(32169..33389)
                     /locus_tag="IV228_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8716963.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33536..35371
                     /gene="lepA"
                     /locus_tag="IV228_001028"
     CDS             33536..35371
                     /gene="lepA"
                     /locus_tag="IV228_001028"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8716964.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35567..>36347
                     /locus_tag="IV228_001029"
     CDS             35567..>36347
                     /locus_tag="IV228_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8716965.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKL"
BASE COUNT        12245 a         6337 c         7789 g         9976 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4861 gaggtcgtga gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc
     4921 tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc
     4981 agaggttgtg acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac
     5041 attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta
     5101 aagaaaagcg tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt
     5161 cctatggtaa actgacaaag ttgagaatgt tagaacagat cgtttaggag gaaacagatg
     5221 tttacgaatg aaataaaaat catgctttat gtggacgatg tagaaaaaaa tgcaacgttt
     5281 tggagagcag ttggctttgc ggaaaaagat cgtcaagaaa tggatgggac gcttataatt
     5341 gaagtaggag ttgaggacag tcaaacctca tttctgttat atgatcgagc atttgttgag
     5401 tcccattcac ctggcacagc tactagtgcc ccatctttga tgttttacag taaagatatc
     5461 tttggactat ataaaaaaat gcagacgcaa tcagtcacat taggtgatat ggtacagctt
     5521 ggagaagaat atgtctttaa ctttgctgat cccgatggca actactttgc ggttacaggt
     5581 aaataataga aataaagact ttgtttttct gtgcgaaaca ggaaagcgaa gtctttttaa
     5641 ttcaaataat aatattatgt aaacaaaaaa ctttccatgc gtaattatac acaagcaaat
     5701 aaatcgtcaa atgcgaacgc cttctttata ctgtttagga acgaaaagaa ggaaggaaga
     5761 aatacatgga ttatcaagta ctgctttatt acaattatac aaaaatcgaa gaaccagagg
     5821 aatttgccaa aaatcattta gccttttgca aatctttgga tcttaaaggt cgtattctag
     5881 tagctgaaga aggaataaat ggtactgtgt ctggaaccgt tgaaaacacg actgcctata
     5941 tgaaagcaat gaaagctgat cctcgatttg ctgatacgat gttcaagatc gatccaagcg
     6001 aaggacatgc ctttaagaag atgtttgtaa gataccgtcc agagcttgta agtttaaagc
     6061 tagaagagga cattgatcca aaccaagtga caggtgccta tctttcaccg caagaattta
     6121 aagaagctat tttagatgaa cagacagtgg tgattgatgc acggaatgat tatgaatatg
     6181 acttgggaca ttttcgaggg gctgtacgcc cagatatacg cagttttcga gagcttccag
     6241 actggatccg cgagcacaaa gaagaattta tggaaaaacg ggtagtgacc tactgtactg
     6301 gtggcattcg ttgcgagaag ttttctggtt ggcttgtccg tgaaggattc aaagatgtcg
     6361 gacagctgca tggcggtatt gctacttatg gtaaggatcc tgaagtccaa ggagaattat
     6421 gggacggcaa gatgtatgtg tttgatgaac ggatcgctgt cgatatcaac caagtagata
     6481 aacaaatcgt cggtcgcgac tggtttgacg gaacaccatg tgaacgatat atcaattgta
     6541 gcaatcctga gtgcaatcgt cagattcttg cttccattga aaatgaagaa aaacatttag
     6601 gagcatgtag catagagtgt gcgaaacatc cgcgcaatcg ctatgttcaa gaccatggaa
     6661 tctctccaga agaagcagaa ctgcggttta cagctttaat taaatagaaa acaatagttt
     6721 tgattgtcga ccatttccaa tatgcgctat actagcgttg aggatggtga tccacatgaa
     6781 atcattgatt tatgctattg gggatgtcca tggcaaacat gagctttttg aaaaacttat
     6841 tcactattat gatccaaaga tccatcagct tgttttatta ggggatctca atgatcgagg
     6901 tcctcgtaca aaagaatgtt ttttattagg aaagcaatta gtcgaagaaa caggagccat
     6961 ttacctccgg ggaaaccatg aagaatattt tttgcaattt ctgcaatcac cggaggactg
     7021 gtatgcgcct tatatgagaa atggtggaaa agaaacgttt gaaagcctga ttcacccagg
     7081 ggcatcggag gaatactccc caactgaaat ctcgatgatg atccaatcga gatattcaga
     7141 actgattaaa tttttagtcc agcgcccctt gtattatgaa tgggaaaatt atctttttgt
     7201 ccatgccggt gtggatttga caaagaaaga ctggcgtaag acaagtctaa gagactttat
     7261 ttggataagg gaaccatttc atgaagggaa aaacaataca ggtaagacaa ttgtatttgg
     7321 acatacgatt acaccaatgc tccatggaga tatgcagaca acagacctct ggattcagga
     7381 tcataaaatc ggaatggacg gtggggctgt attcggaggg tcattgcatg gggttgtttt
     7441 tgacaaggaa ggtattcttc aagatattga atatcagaac aacgaaggac cttggcatgg
     7501 aatctgacta atggaaagaa tgaaggagct gtgactttgt tgcggctctt tttattttta
     7561 tagagaatat tgtttctttc agtagtttaa cccaggataa aaagtatgat agaataaaga
     7621 agcaattttt agcagaaaag aggcatttta atgacggaaa atttgaacca gacgatcatt
     7681 cagcttgtac aaaaagaact ttcagactat cgtcctaagc agttgactac tgtacttaat
     7741 ttgttaaatg aaggaaacac tgttcctttt atcgcgcgtt accgaaaaga aatgactgga
     7801 agtcttgatg aagtccagat tcgtgaaatc gaagagcggt atgcctatct tgaaaactta
     7861 gaaaaacgta aaactgaagt gattcggcta atcgatgaac aagggaaact gactcccgag
     7921 ttagaagcag aaatcaccca agccgtgaaa atgcaacaag tggaagattt gtatcgtcca
     7981 tacaaacaaa aacgacggac aaaagcaacg attgctaaag aaaaaggact tgaaccactc
     8041 gcgaagtggc tgatgcagct tacagatgga gaggtgcaat ctgaagcaga aaaatatatt
     8101 gacaaagaaa aagaagtctc ttctgccgaa gaagcgttgc acggtgctca tgagattatt
     8161 gctgaacaag tcagcgataa tgcaaaattt cggacttgga ttcggtctta tacgtataac
     8221 aaaggtatgt atgtcagcaa tgtcaaggat gagcaagcag atgaaaaagg cgtatatgaa
     8281 atgtattatg actttgcaga acccgtccat aaaatggttt ctcatcgaat attagctact
     8341 aatcgtggag aaaaagaaga tattctgaaa gtattctttc aagtagatga agcagcaatt
     8401 ttagcttact tggatcgcca aatagtcaaa aatcctgcct ctccttcttc ttcattcgtc
     8461 agagaagcct atcaggatag ttataaacgc tttattcagc cagcaatcga gcgagaactg
     8521 cgcaatgaat tgactgaaaa ggcagatgag caagcaatcg ctattttcgg cgaaaacctc
     8581 cgtaatcttt tgttgcaacc gccactaaaa ggtaaagttg tcctgggatt tgatccggct
     8641 tatcgtactg gatgtaaact agcagtcgta gatgcgacag ggaaagtttt agcaatcgaa
     8701 gttatttatc ctcataaacc tgcagcgcaa gcaaaaagag aagctgctgg accagctttt
     8761 atccaattaa ttaataaata tcaagtagat atggtagcta tcggaaatgg tacagccagt
     8821 cgggaatctg aactttttgt ggctgagcag ttgaaatccg ccgaccataa aacctattac
     8881 gctatcgtca acgaagcagg cgcttctgtt tactccgcta gcgaaattgc acgaaaagaa
     8941 tttccgcatc tacaagttga agaaagaagt gctgtttcta ttgcccgtcg cctgcaagat
     9001 cctttagctg aattagtcaa aatcgatcct aaagctgtcg gagtaggcca gtaccaacat
     9061 gacgtttctc aaaaacgttt ggctgaacaa ttggattttg ttgtggaaac agcggttaac
     9121 caagtaggtg tggatgtgaa tacagcaagc ccgcagctct tgcagcatat atcaggattg
     9181 aataaaacaa ctgcccaaaa cattgtcatt tatcgtgaag aaaacggaga attcaccgca
     9241 cgaactcaat tgaaaaaagt gccacgctta ggaccaaaag catatgaaca agcaatcgga
     9301 ttcttgcgag tccctggtgg gaaaaacatt ttggacaaca caggtatcca tcccgaaagt
     9361 tattctattg caaaagaaat attaacttca gtccaacttt cagaaaaaga gcttggtaca
     9421 gaagaagcag tagaaaaatt gacacgatta tccgtagaga aactggctga aagcctatca
     9481 gtcggagaag aaacgttagc cgatatttta gctgggctga cacaacctgg acgagatatg
     9541 cgtgatgaga tgccagcgcc cttattacgt acagatgtat tgagtatgga agacttgaaa
     9601 ccagggatgg aattgactgg aacggtccgt aatgtcattg acttcggcgc atttgttgat
     9661 atcggtgtga agcaagacgg acttgtccat atatcgaaac tcagcaaaaa attcgtcaag
     9721 catcctacag atgtcgtgtc agtaggagat atcgtaacag tttggataga acaagtcgat
     9781 acgaaaaaag gcagaatcag tttgacgatg ctttcccctt atgaagagta acaatgagga
     9841 gtaacaaaaa ttgacacaag aagaactgca gcagttagta gaagagatat cattaaaaag
     9901 cttccagctt cctttcaagc accaagcaat gttcaatcca cgcttacgta caactggcgg
     9961 gcgctatcat ttaaacgatc atcatttaga ttttaatgcg aagctatttg aagagtcaga
    10021 cgaagagacg attgcaggaa tcatcaaaca tgaactttgc cattatcatc ttcatctagc
    10081 tggaagaggg tatcagcata aagatatgga tttcaagtat cttttgaaaa aaacaggagg
    10141 gttgcgctat gcaccagcct tgacgaagaa acagataaag atggagtatt atcgatgcca
    10201 agattgttct gccacgatct gccgcaagag aagaatcgat gtacgcaaat atcgttgtgg
    10261 aagatgtcat ggagaaatca aatggattga gacaaaaaga gaagacttta atgaagcaca
    10321 aaaaatcaga tgaaaggata gaaggccttt ggaaaaaagt gagcagagga atctaaaaga
    10381 ctggaatcag ctattaaatt ttttagccgc acctgaagag ggaaattttg aggaactgac
    10441 ggcacctaca cttattttag ctggaaactg tcttcctatc ttagctgata aagcagcaca
    10501 aatgtattta aatggccaag tagatcaatt gtttttggtc ggaggggtgg ggcatgcgac
    10561 tagaatcctt tatgagaatt ttgaaaagca aggatttcat tttgaagatg gcatgagtga
    10621 aagtgaaatc tgtcgtcagt atctaaaaga agtctatgat ttgcccgata aggcttttct
    10681 gatagaatcg aagtctacaa attctggaga gaacgccact ttttcactgg agattcttca
    10741 ttccttagga gctgtaccag aaaaaatctt attgatgaat gatccgacac tgcaacgcag
    10801 gactcgagca acttttgaga aagtatggca aaatgaacaa actattttcg cgaatgctgt
    10861 gccatttgtg ccggaaatcc ttcacttttc tgaagagatc atctttacag caaaagaact
    10921 gaatcatcaa tggcctaagg aatatttcca tgcgctagta ttaggagaaa tggaacgtct
    10981 ccatgacgat gagaacggct atggtccgaa aggaaaagat tttattcctc acattgatat
    11041 atcagaggaa gtttggtctt cttacaccag aattaaagag tcaataaaaa cggatttttc
    11101 tcgtacttga ccattttagc aagcgaatcc tcctttccaa ataaatttgg ttgaaatttt
    11161 caatcgttta taagataatg aggagcgtgt tgaatgtaat agctaaaaga atggacgcta
    11221 gataagagaa gaggattcaa atggaaaatc aaaataaatt gtatcatttt gttggtatca
    11281 aaggttcagg aatgagctcc ttggctcttg ttttacatga acaaggtcta aatgtccaag
    11341 gatcagacat tgaaaaatat ttctttacac aaagagattt agaaaaagca aatatcacga
    11401 ttctgccatt taacgcagat aatgtaaaac cagggatgac gatcattgca ggaaatgcat
    11461 ttcctgattc acatgaagaa atccagcgag caaaagaatt aggtttggaa gtgattcgct
    11521 atcacgactt tattggtcat tttatccaaa attatacgag tatcgctgta acaggctctc
    11581 acggaaaaac aagtacgact ggactgctct ctcatgtatt gagtggtgtc cgtccaacaa
    11641 gttatctaat tggagatgga acaggacatg gtgatccgca agcggaattc ttttcatttg
    11701 aggcctgtga atatcgccgt catttccttg cctattcacc agactatgca atcatgacca
    11761 atatcgattt tgatcatccg gattattata caagtatcga cgatgtattt acagctttcc
    11821 aaacaatggc tggacaagtg aaaaaagcta tttttgcgta tggtgacgat gcttatttac
    11881 gcaaattaaa agcaaatgtg ccgatttatt attatggtgt cacagaaaac gatgatatcc
    11941 aagcacgaaa catcgaacgc acaacttctg gttctgcttt tgatgtctat catggggatg
    12001 aatttgttgg gcacttcact gtaccagcat tcggtaaaca caatatctta aatgctttag
    12061 gtgttattgc agtagcttat ttcgaaaaac tggatctgaa agaagttgca gaagaaatgc
    12121 tgactttccc aggagtaaaa cgtcgattca gtgaaaagat cgtagcagac atgaccgtag
    12181 tcgatgatta tgcgcatcac cctgcagaga tcaaagcaac gattgatggc gcacgccaaa
    12241 aatatccaga taaagaaata attgcagttt tccaacccca tacgtttacg cgtacgattg
    12301 cattgatgga tgaatttgca gaagcacttg atttagctga taaagtttac ttatgcgata
    12361 tttttggttc ggctcgggaa gaacaaggaa acgttaagat tgaagacctt ggagcgaaaa
    12421 tcaaaaaagg tggagaagtc atcaaagaaa ataatgtatc tccattgtta gattatcatg
    12481 atgcagtagt aatcttcatg ggtgctggag atgttcaaaa atttgaacaa gcgtatgaaa
    12541 aacttttaag cagcacgacg aaaaatgtac tgtaatacaa ttactgcagt gtccttataa
    12601 aaatagaaag agggggagtg gcaaaaatct tgccaagccc ccttttttgc aacttatgat
    12661 aaacggtgtt caaaagcttg catctgcggt aataagccca atcaaatcaa aaacatgaga
    12721 agctgttttt tcttggctgt tcttattatt tgatgcagga cagctttttc acgacctctg
    12781 taaactatag gaatctgtta aatcaaggga gcagaacttg ccttttacgc tacttttggt
    12841 acaataatgt atgattaaaa ataaactgga gggtttctat cttgaacata ggtaagccga
    12901 gaaaactagg aaaaacaata aatgaaatcg aggaaggcga ctcgttatcc ttaacagaat
    12961 ctattgaaga taaccagctt ttgctgtatt tgggattgac gaatgatgca aatcctttgt
    13021 atatccaaca tgactttgca caaaaaacag aatatcaaca gccaatcgtt ccatcagtga
    13081 tgctgatggg gatcatcaca agtgcaattt caaagcatct tcctggacca ggatcacatg
    13141 ttgtcaattt ttctgtcaat tttattgaac cggttttcca ttatgaaaca ctgacttttc
    13201 agtttgaggt cataaaagta gataagatga aggatgtggt gacgatttct gtagaagcgg
    13261 agaatacgaa cgaagatcgt gtgttggatg ctgtcgttat ggtccaaccg ccacaagttc
    13321 ctgaattgga aatagcaatc gaaagtgaag gtgaattgaa tgaataatcc aagtacatca
    13381 atgtcaggta tcaatcgttt ttatgcaaaa gtttatggat ttttaggtat gggacttgcc
    13441 cttagtgcag tcacttctta cttggctctt tgggtatttc ctttgcaatt agcaactttt
    13501 atcaataatt ttccactagg ctttgtgggt ctgtggatcg ttgaactgat tttagtaatg
    13561 gttctaggcg taaaagcaca aaagaatccg agcttagcaa tcggtggatt tattgcctac
    13621 tctcttttga atggcataat attagcggtt actcttgcat actacgatat tggaacagta
    13681 acgcaagctt tcttgacagc tactttgatg ttcatgggga tgtctgttgt cggagtaaca
    13741 acaaaaagag atcttagcgg ggttggaaga gcaggacagg cagctttaat cggagtgatc
    13801 attgcactag tcttgaacat cttcattttg agaagtagcg tagttgagct gttcatctcc
    13861 ctactgttag tcgtcatctt tgcagggatt acagcttatg ataaccaaag catccgtaat
    13921 atctatgtcc agacaaacgg aacagcacat agtggaattg ctgtattcat ggcattgaag
    13981 ctgtatttgg actttatcaa cttgttcttg gcgtttctga gaatctttgg acggaacaac
    14041 taaaaaagaa aaaaattcat cagttggggc tataccgaat tatttggtat ggtcccgctt
    14101 tttcttttcg cattccgcag gattctttgt tagaatgaat aagaacttta cagtaaggag
    14161 ttaagaagat gacaaaaaat aaattattac tcgttgatgg gaacagtgtg gcttttcgcg
    14221 cattctttgc gcttcacaat tctcttgaac gtttcaaaaa tagaaacggt cttcatacaa
    14281 atgcaatcta tgcattcaat acgatgtttg aaaatgttat gcaaaaagag caaccgactc
    14341 atgttctggt tgcgttcgat gcaggaaaaa cgacttttcg gacagaaatg tatgcggagt
    14401 acaaaggtgg aagatccaaa acacctggcg aattcaaaga acaaatgcca tatatacgtg
    14461 atttgttgac aggactaggg gtacagtatt acgaattgcc caattatgaa gcagatgata
    14521 ttatcggtac tttggcagaa aaagtagaca aagatcaatt tgatgtcgtc gtgctttcag
    14581 gtgatcggga tttgactcaa ttagctagta aagaagtaaa agtggatatc actgtaaaag
    14641 gtgtcagcga tatcgaaagc tatacaccag aacatgtagc ggaaaaatat gatgggttga
    14701 cgccaaaaca gattattgac atgaagggac tagccggaga tgcctctgac aatatccccg
    14761 gtgtgactaa aatcggtgaa aaaacagcta tcaagctatt gaaacaatat ggctcagtcg
    14821 aaggaattta tgaacatatc gatgaaatga agcaaagcaa aatgaaagaa aatctgataa
    14881 atgacaaaga gcaggctttc ttatcgaaaa aattggcgac catcaatact tcagcgcctg
    14941 tggatgtctc tatcgactca ttgaagtatg aagggaaaaa tctcgataaa ctcgttccgt
    15001 tttataaaga aatggatttc aatcagttcc tttctaaatt gaatatcgta gaagaagaag
    15061 taaaaatgga tgatattttg tttgaagtcg ttcatgaatt caaagaggaa atgtttacta
    15121 cggatatggc gttgtacgtg gaaatgatgg gcgacaatta tcacacagag gaaatcgtcg
    15181 gagtagcgtg ggggacagaa aagaaaattt atgtcacgaa cgatctatct gtattcgaaa
    15241 accgagcgtt ccattcttgg atcactgatc cgactcggct gaaaaaagta tatgatgcga
    15301 aacgaactta tgttgcttta aaccgctata ctggaaaaac gaaaggaatc gattttgatg
    15361 tcttgctagg tgcatacctt ctcgatacga atgaaaaaag cacagatatc gaaggaatag
    15421 ctgctcatta cggatacaac gatatccaat cagatgaagc tgtatacggc aaaggggcga
    15481 aaaaaggttt accagaagaa gaggaactct tttttgctca tttagcgcga aaagtagctg
    15541 caatcaatgc gttgacacca aaattgagcc aagaactggc agaaaaaaac caagaagatc
    15601 ttttccggaa aatggaactg ccattgtcta tcattttggc agagatggaa ataagcggga
    15661 tcaaagtaga tgcgactcgc ttacaagaaa tgaagggcga gttctctgct cgcttaagag
    15721 agatcgaaca aaaaatctac gaagaagccg gcgaagaatt caatttgaat tcaccaaagc
    15781 aattaggagt gatcttattt gaaaaaatgg gattacccgt tatcaaaaaa acaaaaactg
    15841 gatattccac agccgtagat gtactggaac agcttcgtga acaagcacca atcgtagagg
    15901 atatcctcac ctatcgtcaa attgcaaaaa ttcaatctac ctatgtcgag ggactgctga
    15961 aagtgatcca atcagatgga aaagtgcata cccgttatgt ccagacattg acccaaacag
    16021 gacgattaag ctctgttgat cctaatttgc agaacatccc gattcgatta gatgaaggga
    16081 gaaagatacg tcaggcattt gttcctagag aaaaagactg gctgatttat tcttctgact
    16141 attcacaaat cgagttacgc gtacttgcgc atatctcaaa tgatgaacat ttgaaagctg
    16201 ccttcctaga aggtcaagat atccatgcca gcacagcgat gcgtgtattt ggaatcgaaa
    16261 aggcagagga tgtcacacca aatatgcgta ggcaggcaaa agcagtgaat tttggtatcg
    16321 tgtatggcat ctctgattac ggactttcac aaaatctagg aatcagccga aaagcagcac
    16381 agcaatacat cgatacgtac tttgaaaaat acccaggtgt caaagaatat atggaaagaa
    16441 tcgtacgcga agcaaaagac caaggctacg tcgaaacgct gtatcaccgc cgtcgatatc
    16501 tgccagacat caattcaaga aattacaata tccgttcttt tgcggaacga acagctatca
    16561 atacaccaat ccaaggaagt gctgctgata ttttgaaaat cgcgatgatc gaattggata
    16621 aacgattaaa agaaacaggt cttcaagcta cgatgctgtt acaagttcat gatgaacttg
    16681 tcttcgaggt accggaaaaa gaacttgaat cattagataa attggtgaag gaagttatgg
    16741 aacaagctgt ttctcttcat gtaccactga tcactgacag cagttgggga aaaacgtggt
    16801 acgaagcaaa ataaacattg tttaagacag agcaatataa tgaaggtgaa aagaaaaaat
    16861 gccagaatta ccagaagtag aaaccgttcg taaaggattg gaacggttag tagtaggaaa
    16921 aacgattcaa aaagttcagg tcttgtggcc caagatcatc gagcaaccag agacacccat
    16981 ctttgaggct tctcttgttg gcgaaaccat ccaatccatt ggacgtagag ggaagttttt
    17041 aatttttcat ctcgatcatt gtgaactgat ctcacacttg cgaatggaag gaaaatatca
    17101 atttacgaaa gaaaatacac caatcgataa acatacgcat gtacttttct tttttgagga
    17161 tggaagccaa cttcgctaca acgatgtacg aaaatttggt cgtatgacaa tcgtcgaaaa
    17221 aggagcaagt gcaacttacc gaggaatcat gaaattggga cctgagcctt tgccagatag
    17281 cttcttatta gcagatttcg caaatggact gaaaaagtcc cacaaagcaa tcaagccatt
    17341 gctgctagac caacgtctag tgactggtct aggtaatatt tatgtagatg aagcattatg
    17401 ggaagcgaag atccatccag aacaaccggc gaacacactg cgccccaaag aggtagaaca
    17461 attacgtcta tcgatcattg atgttttgga tcgtgcaata gaagccggag gtaccacgat
    17521 tcgcagttat ttgaatgcat taggagaatc aggcggattt caagtagcac tgcatgtcta
    17581 tcagcaaact ggcaaaccat gtatacgttg tggaacgccg atcgtgaaaa caaaagtggc
    17641 acaaaggggg acgcactatt gtccgaattg tcaaaggcta aaaatcgcaa agtaggattt
    17701 gttttaggtt tgacgggtgg aatagctacc ggtaaaagta cggcagccaa agtctttcag
    17761 tctcatggat ttcctttgat cgatggcgat gtgattgcca gagaaactgt tgagcctgga
    17821 acgcttgctc tgaggaaaat tgtatcagtg tttggccaag aggtattgca gcctgatggg
    17881 caactagatc gagggaaact tgggatgatt gtttttccat caaaggaaaa acggcaaaaa
    17941 cttgatcagc tattagatcc gtttatccga aaagcgataa aagatcaaat cgcctctcta
    18001 agttccaatc atcctttagt aattgtagat atcccattgc tctacgaagg tcattatgac
    18061 cactatatgg atgcagtagc tgtcgtttat actacgccag aaactcagct tcagcgtttg
    18121 atgaaacgta atcaactgac gctagaacaa gcgcagcaga gaattagtag tcaacttcca
    18181 atcgaagaaa aaaagcagcg tgcggatatt ttgttcgaca acaatggaac aaaagaaata
    18241 cttgtgggac aaatcgaaaa ttggcttttt gctaatcgct ttctttgagt atctgtgtca
    18301 attttgctat cctgagtata tagaaagtga gggatagcga tgggatcatt aaaaaatcaa
    18361 aaggataaag tcgtaggtaa cgcaaaagaa aaaatcggcg agatgctcaa taaagaaaat
    18421 ctaatcgatg aaggtcgtac gcaatcagaa acagcagaag aaaaagaaaa gcagtaccaa
    18481 gcaagaatcg atgaaataga agaccagcag gcagaagaag aagcgaagcg gcaagatttg
    18541 actcaagaac gaaaaaatcc gacacctgat cgtagtaaaa caccgctccc agaaagtgac
    18601 cacattcccg atgagcaaaa aatcgaggaa cagcgaatga aacactatac aggaatcgaa
    18661 gagaagttat aaaatagtaa aaaatcgaga tgtgacttga agcagatgag aaggtgttca
    18721 tctgcttcaa gttttttgtg ctagatgact aaatcagctt ttgtgtaagt tatttctcag
    18781 taatcaaggt gatcagtatt ggaaaattga aatttagtaa ggacagggga cacaattacg
    18841 catgaaagta gaaaaaattg ttcttatatc cgtgtttttt gggtaggaca gtcgcgtttg
    18901 tgatttgttt cgtgttataa tacaactagt tgtaaagaaa gacacatcct ttatataaaa
    18961 tgcgatgcaa gaggtgagac aaaatgagat gtcccagatg tcaacataat aattcacgag
    19021 taatcgacag ccgtcaagca gatgatggac gtgcgatccg tcgccgtcgc gaatgtgaga
    19081 attgttcgtt ccgattcact acatttgaac gaattgaagc tgctccgtta ttagtaatca
    19141 agaaaaacgg ggaacgcgaa gagttcaatc gcgataaaat tttaagagga ctcatccgtt
    19201 cagcagaaaa aagacctgta gctatggaac aaatggaaca aatcgtcaac catgtcgaaa
    19261 accgcgtacg cagtctcgga gaaaacgaag tatcgacgaa tctaattggt gaatatgtga
    19321 tggaagattt agttgattta gacgaaatcg cttatattcg ttttgcaagt gtttatcgcc
    19381 agttcaaaga tatgtctgtt ttcttaaaag agttgcaaga tatcgtggat aaagcaaaag
    19441 aaaataataa agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg
    19501 gaaaatgctt ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg
    19561 ctgagagaag acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa
    19621 gccttgagta tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct
    19681 gaagggatgc atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat
    19741 gcaagaaaaa agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat
    19801 ctgggacctc gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa
    19861 gactctgttc tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt
    19921 acccaattct ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa
    19981 tttttagatg tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa
    20041 tcgctgaata aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca
    20101 ttcgattggc agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat
    20161 ccaatcatca aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt
    20221 ggcgaactga tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg
    20281 caaaaagcca ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg
    20341 gtttctgaaa acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag
    20401 ctgataaaag aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa
    20461 aaaggcggat atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca
    20521 ggtctttcca gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat
    20581 gctagcttaa atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc
    20641 gccacagcag aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca
    20701 aaacaaaaga agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt
    20761 cctgattggg tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag
    20821 attgaccgcc gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg
    20881 taggcaaaga attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa
    20941 tgatcgctga agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag
    21001 aattgacaca agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa
    21061 agcgtaaata tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga
    21121 cgttgaattt tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc
    21181 aaagacaaga agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat
    21241 cggcttcttt tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc
    21301 tagattttgt agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag
    21361 taggaagttt tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga
    21421 ttgctggatt caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg
    21481 caatcggaaa agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga
    21541 tgatcgatga cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg
    21601 ttatccttca atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa
    21661 tggatgaatt ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag
    21721 cgaaacggat aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca
    21781 accgacgcaa tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc
    21841 aggtcagcga atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg
    21901 cagaaactta aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg
    21961 tctatttttt cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc
    22021 gattgatgcg ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt
    22081 tgtcggaacg attgaaagta tcactcaagt agaagcagaa gaagaaacgc ttcagttgaa
    22141 actcgtcgat gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga
    22201 cggagtgatc ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat
    22261 cggtgacaaa gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc
    22321 gcaagttcct ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt
    22381 tcaaaaataa gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg
    22441 gaattttttt attcctagtc tccttatttt atttggaaaa tgctataatt atctagaagg
    22501 agtgaagcta tgaaccattt agatttatct cagcgcttag ccgcagtggg tgactttgtc
    22561 cctgaatccg ctcgtttagc agacattgga tcagatcacg cttacttacc cgttgcattg
    22621 atattaagag gaaaaatcga atatgcagtt gctggtgaag tcgttcaagg tccttatgaa
    22681 tcagcacgta agcaagtcaa aaaagatgga ttagaagacc gtatcaccgt acgccttgca
    22741 aatggtctag atgctatcca gccacaagat ggtattacag ctgtcacgat tgcgggtatg
    22801 ggaggttcac tgatccgaga tatcctagaa gctggcaagt tgaatcaacg cttaagcgga
    22861 caagaacgtt tgatccttca gccaaacatc ggagagcgta cattacgatc ttggctgcaa
    22921 gaaaatcagt ataaaatcca atcagaaaaa atacttgaag aaaataaaaa aatatatgaa
    22981 atcattgtag ccgaaaaaga acaaaaacca gttacttaca atgaaatgga gctgatgttc
    23041 gggccatttc tgcttgaaga gaaaagtgag atcttcaaat caaaatggac aagagagctt
    23101 caacaaagaa aagctatccg tgaacagctc aaacaagcag ctaatcaaga ggcacgtcaa
    23161 aaacaaatcg agcaggaaat ccaaatgatt gaggaggttt taaagccatg acaatcagcg
    23221 gacgcacttt catccaaaag tttgaaagct attgtcctca gtggctagct gaagaaggag
    23281 acccagtcgg actgcatatc ggaacattgg ataagccaat ccaacgagta atgatgacat
    23341 tagacgttcg tccagaagtg gtcgaagaag caatcaagaa aaaaatcgat ttgctgattg
    23401 ctaaacatcc tccaatcttt cttcctgtaa aacggctggt tactgatcag ccacaagaaa
    23461 aaatgtatgc agatttgttg aaacatgata ttgccgtcta tgcagcacac acgaacatgg
    23521 atattatttg ggatggactg aatgactggt tttgtgaact tctcggtatt gaagtggaga
    23581 gttatttagt aaagactcat gagattcatt ataaaaaact ggccgtttat gtacctgtgg
    23641 atcatgcaca gaaaatgcgt gaagtattag ctgcagctgg tgctggtaca caaggtgatt
    23701 ataccggtac aagttttacc tcgattggac acggtcgatt tactccggaa gctggtgcac
    23761 aaccagcgat tgggaaagta gggaagacag aacaagttca agaggcaaaa gttgaggtta
    23821 ttttgccgga aactatcgaa aaacaagtga ttcaagccat gcggtcagct catccttatg
    23881 aggaacctgc ttatgatctt ttcgctatag atgaaccagt cgaaatgttt gggttaggac
    23941 gagtaggtga attgccgcaa gaaatttcta tagaggcatt tgtagaacaa gtaaaagaag
    24001 cgttccaact agatggactg agaatagtcc agccaaaaaa tgcaaaatcc tctgtgaagc
    24061 gcattgcgat ttgcggtgga agcggtgaaa aattttatcc gcaagcaatc gcccaaagag
    24121 cagatgtcta tatcacggga gatatctatt atcatacggc tcatgatatg caaagcgctg
    24181 ggttgattgc aattgatccc ggtcattata ttgaatcgtt atgcaagcaa cgatttatcg
    24241 agaaatttga atcttggaaa caagaagaaa attgggatat taactttttc gtttctgaaa
    24301 caaatacaaa cccatttcaa tttaattaaa aggagagaat actatgtacg aaaatctttt
    24361 gccacgtttt ttacgatatg tcaaaacaga aacacgctcc gatgcaacaa gcaaaacgac
    24421 tccatcgact cagacacaag ttgcttttgc gcaagaattg aaaaaagaat tagaagagtt
    24481 aggcttagct gatgttttct ataatgaaga taacggattc gttatgggaa cattaccaag
    24541 caatgttgat catgaagtgc gttccattgg atttatttcc cacatggata cagcagattt
    24601 taatgcagta ggagtcaatc cacaaatcat tgaaaactat gatggcgagt ctacgatcgt
    24661 actagatgcg gaagaacgct atacattgaa tacaaaagat tttccaaatt tgaaaaatta
    24721 tgaaggccac acattgatca caacagatgg cagtacatta ctgggagcag atgacaaatc
    24781 agggattgcg gaaatcatga ctgcaatgga gatcttgatc aataatcctt ctatccctca
    24841 cggtaccatc aaagtggctt ttggtcctga tgaagaaatc ggtgtcggag cggataaatt
    24901 cgatgttgag caattcgatg tagattttgc ttatacagtc gatggtggtc ctttaggtga
    24961 gttgcagtat gaaacgttta gtgctgctca agcaaatatt acgatccaag ggaaaaatgt
    25021 acatccaggg acggcaaaag atacaatgat caatgctcta cagttagcaa tcgattttca
    25081 taatcagctt ccagccgatg aagttccaga aaaaacggaa ggatacgaag gtttcttcca
    25141 tttgatggca ttggatggga atcctgaaga agcttctatg agttatatca ttcgcgatca
    25201 tgatcgtgaa aaattcgaag cacgcaaagc acaaatcaca catatccaag aagagttgaa
    25261 tcagcgattt gatcaagaac gaatcaaagt agatatgtat gatcaatatt acaacatgaa
    25321 agaaatcatt gaaaaagata tgagcatcgt ggaattagcg aaacaagcaa tgatcgagct
    25381 tggtatctct ccagtgatcg aaccagttcg tggaggaaca gatggttcaa aaatctctta
    25441 catgggtatt ccgacgccaa atctatttgc cggaggagaa aatatgcacg gacgattcga
    25501 atttgtttct ttacaagtaa tggaaaaagc aacagacgtg attgtaaaaa ttgcagaatt
    25561 gaacacaaag agttaaaatc gaaaaagaac agcttatgac aaggcgttcg tcccaatgat
    25621 ggaatgtcat ctgtctaagg ctaaagggca ggacaatgga attggttgtc ccgccctctt
    25681 ttttgaagag taaataggag gtaaagaatg gaaaagctgc tagtttttta taacaaaagt
    25741 tcagggaaag atgagggtga gaaattagca cgctggtttc aagattatgt aaaagaaaag
    25801 cagccagaat tatatgtaga actagctgaa actggcccat cgattgatga tgcggaatta
    25861 gtcaagaaag cagaagccat agaagctgat actttagtaa ttataggtgg agacggcacg
    25921 attcatcata tcgtccaagc gttccaaaaa acattatctc aataccatgt cggattactt
    25981 ccaggtggaa ctgtcaacaa ccttgcacgt gttcttgaga ttcccctcaa aaaagaagaa
    26041 gctgctgacg tgatcctgga aggcaatgta cggaaaatag actatggtca agtcaatgat
    26101 gaagtcatta tctctacact gacaatcgga atcttagcag atacagcagt ttggatcagt
    26161 caagaagaaa aacagcgtta cggctcgtgg atctttatca agaagttttt taagttattg
    26221 ctgaagaaga aaaaatatca tttggattta gaaacagacg aggagcattg gaaaggaaaa
    26281 tcccaactgt tgacagtcac gatgtctaac tccgtgggcg gttttacaaa ttttgacgac
    26341 tcagcgaccc tagatgatgg aaaatttcat gtaacgatcg taccttcctt agatattttc
    26401 cgatttattt tttatttgcc tagaatcatg agaggcaaat tctatacgat ccccggcatt
    26461 acttatttca cagccaaacg catgaaaatc caagcgcagg aaaaatctgt gggcactcga
    26521 acggatggag acacgacaga tgatcttcca gttgaattat ctgttgtatc aaaaggatta
    26581 acagtttatt caccaaagaa agaagaaaaa gcttagaaat agtagttaac agaaagttgg
    26641 aagaagagta atggatgtat tgttacacaa tcctttgtgg ttttaccttt tcattgtgaa
    26701 tttttactta ttttgtctga tggggtatga taaacatcag gctagaacta gcggctggcg
    26761 tgttcctgag aataatttga tatttgtcag cctgttaggc ggtggtttgg gaggattgat
    26821 ctctcgaaaa gttttccatc ataaaactcg gaaaaagaag tttactatag gattcgctgc
    26881 aggtgtactt gttgatttga ttctcattta ttttttccat taaggagcag gagatgtcgt
    26941 ctaaaggaac gaaaactaaa atcattctga atctgctttt gctgattgtg atttttgcca
    27001 ttatttatta tttaatcaat caatcatttg cagatatctt tgccgaattg ctgtctacca
    27061 gtgttgttgt tttggcagcg gctgttatat tagggacagt ttaccaaatc gctgaagggc
    27121 gctcaatcaa agaaattgct gaacctttcg cgccgaattt tacaacgaaa gatgggttct
    27181 tgacctctag ctatattgcg ttttaccgaa tcgtttcgtt tggtacagga acgctgatct
    27241 cggagattta tttttataac aaaaaaggaa tgaagctatc ccagggagca ggggtgacag
    27301 cactccatat gattatgtac aaactggcag tgatcactta tgcattagct ggcttggtca
    27361 tccaattctc tcttttttat tctaatggtc catcaatgat ctggtttatc atcgctggta
    27421 tccttttgac gatcgtgatt gttgcctttt tattggcgat gtccgtcagc ttgaatgttc
    27481 aaatattttt tgtcagctta tcgaataagt tatttaaaag tgagcgtttg cggaaatggg
    27541 tggatacatg caatcagcag atctactctt taagggaaac ggtccaaacc attatccaag
    27601 acaggactgc gctattgcgg atttatctat ggaatatgtt aaaacttgta ttttggtatg
    27661 tacttcctta tttgattctg attgaaaacc atcctaaaat agattttcta ctgaccattt
    27721 cgtttatcag ctttgcagta gtgctttcag gggttattcc tacgcctgct ggtattggtt
    27781 cctttgaatt tgtctatttg ttattattca gaccagttgt tggaacagtt gacgcagttt
    27841 cttcgctttt actgtatcgt ttcagttcct ttatcttgcc atttctctat ggatttgtgt
    27901 atgtgattgc tgaacggcga agagtgatca aagaagaaat aatagaagta aaaaaagaga
    27961 ataaagaaaa tcaagggtag gtcttttata agatctaccc tttttatcaa atcaaagaaa
    28021 aacgttatgg ataagataat tttacgtttg tttggctctt tttttatata catacaatca
    28081 attttgtttt tttaagattt atataagtcg agaaaaaaag tcaaaaaact atgtgtatat
    28141 tttgaccaaa attgaccaaa gcgatataat agtttttgta aagatcaaag aaataagtga
    28201 tcttaccttt aattaggagg gtatacctgt atgaatattg agaaaatgac tacgacgctt
    28261 caagaagcga ttgctgaagc tcaacagatt gcggtcactc gaaaacatca agatatcgac
    28321 attgcccatg tatggaaaat ctttttacaa tctaatcatt ttggtcgaaa tttctatacg
    28381 gatgccggat tggatgtcga atcttttgaa catgaaattg atcgtctact ggatgagtat
    28441 ccagttgttt ctggtggaaa cgtccaatat ggacaaaacc tcagccagaa tttctttcga
    28501 ttattgaatg aagcagacca aatacgtgaa agcttcggcg acgagtttct ttcaacggaa
    28561 gtcgttattt tagcgttgat gaaattaaaa aattatccgt taactgttta tttgaataaa
    28621 aacggattat ctgaaaaaga attgcgtaaa aacatcgaag agatgagagg aggagaaaga
    28681 gtgacctcac aaaaccaaga agaacaatac aaagcattag aaaaatacgg cgttgattta
    28741 gtacagcaag tcaaaagcgg aaaaatggat ccaatcatcg gtcgtgatga agaaatccgt
    28801 gatgtcatcc gtattctttc acgaaaaaca aaaaataatc ctgttctgat aggagaaccc
    28861 ggtgtaggga aaacggcaat cgttgaaggg cttgctcaac gtatcgtacg caaagatgtg
    28921 cctgaaaatt taaaagataa aacgattttt tcattagata tgggtgcatt gatcgctgga
    28981 gcaaaattcc gtggtgaatt tgaagaaaga ctaaaagcag tcttgaaaga agtcaaaaaa
    29041 agtgatggac gtatcctctt gttcatcgat gaaatccaca atatcgtagg ggcaggaaaa
    29101 acagaaggaa gtatggatgc cggaaatctg ttgaaaccaa tgcttgctcg cggtgaatta
    29161 catttgatcg gtgccacaac tttagatgag taccgtcaat atatggaaaa agacaaagcg
    29221 ttggaacgtc gctttcaaaa ggtattagtc aaagaaccaa cagtggaaga tacgatcagt
    29281 attctgcgtg gattaaaaga acgttttgag atccaccatg gtgttaacat ccatgacaac
    29341 gcattagtag cagcagctac tttatctgac cgttatatta ctgatcggtt cttaccggac
    29401 aaagcaatcg atttgatcga tgaagccagt gcaacgattc gggtagaaat gaactctatg
    29461 ccaacggaat tagatcaagt tactagacgt ttgatgcagt tggaaatcga agaagctgca
    29521 ctgaaaaaag aatcagatga tgcaagtaaa aaacgcttga aaaatttgca agaagaatta
    29581 gctgaacttc gtgaagaagc aaatgcgatg aagatgcagt gggaaacaga aaaagaagaa
    29641 gtcaattctg tttcagcaaa acgtgctgaa atcgataaag caaaacacga attagaagat
    29701 gcagaaaata actatgattt agaacgcgct gctgtattgc gtcatggtac gatacctcaa
    29761 ttagaaaaag aattgaagga attagaagct aaagcaaaag acagcgagat taagatggtt
    29821 caagagtccg ttaccgaaaa tgagattgct caagtcgttg gcagattaac gggtatcccc
    29881 gtaacgaaac ttgtagaagg tgaacgagaa aaactaatta aattgaatga aacacttcat
    29941 aaacgagtga ttggtcaaga tgaagcagtt gatgcggtca gtgacgcggt aattcgttca
    30001 cgggctggat tgcaagaccc aaatcgtcct ctaggttctt tccttttcct aggaccaact
    30061 ggtgtcggaa aaaccgaatt agcgaaagcg ttagctgaga acttgtttga ctcagaagat
    30121 catatggtac ggatcgatat gagtgaatat atggaaaaac atgctgtgtc tcgtctagtc
    30181 ggagctcctc caggctatgt aggttacgaa gaaggtggtc aattaacgga agctgtcaga
    30241 cgaaatcctt atacaatcgt cttgttggat gaaatcgaaa aagcgcatcc cgatgtcttc
    30301 aatatcttgt tacaagtcct agatgacgga cgcttgactg attcaaaagg gcgagtagta
    30361 gatttcaaaa atacggttct gatcatgacc agcaatatcg gttcacagtt actgctggaa
    30421 ggcgtgactg ctgatggaac gattccagaa gcagtggctg aacaagtcaa cacgctatta
    30481 cgtggaaact tcaaaccaga attcttgaac cgaatcgacg acacgatttt atttacacca
    30541 ttgagcctag acaacgtgaa aggaatcgtt gacaaaatgg tggcgcaatt ggcacaacgt
    30601 ctggaacatc aagaaatcct gctgacaatc agtgatgaag ccaaaacgtg gattgctgaa
    30661 aatgcttatg aaccagcata tggtgcaaga ccattgaaac gatttatcac taaggaagta
    30721 gaaacaccac tagctaaaga aatcgttgca ggacatgtta tgcctaaatc aaaagtaacg
    30781 attactttac ttgatggaca acttcatttc aaaacagaag agctagaaga aatcgtctaa
    30841 tcttccattg agactataaa agaaaaaagg ggtctgacac aactagtgtc agacctcctt
    30901 ttttcaaata aacagtgttc cagaagtagt ttcttcgaaa aataagccga aatttcttga
    30961 agctgcccgc ttctgtcaca acctctttta ttaggtattt ttcacttttt ctaatttata
    31021 actttcaaag taacgctcat cttttttact ttgagaccat gcaaacaatc ccattaaggc
    31081 agcacctatc gtatttgtca gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac
    31141 aaacagtgtg ccatcagaag cagcaaaacg ttgaagattc atgcctaaga actggtcgca
    31201 taagaattcc cagaactccc aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa
    31261 gaggaacaac caaggactgg tttttgatat ctcagcatct ttcatcaagt aaccaatcaa
    31321 tccatagcca agagctgtca ggaccattgg actcaccgca tgtaagattt tgtcccaaaa
    31381 agagatgata ctgatcaaat gcatccctgt gcctaaaaag acagagatga acaagaaaaa
    31441 ccagtaaaac aaaacgattg ctgctggtat tttaagtttg aaaactttca aaatgacttg
    31501 tggcatgaaa atcaatgcaa tccctaataa agattccacg ataaatgtga ttccttgaga
    31561 agtcgaatat ttttgtgtga taaattcata aaaattatag cagacaacga atacaccaaa
    31621 agcaagtaaa gctatttgca cctttttgat ttctttaatc actgaaagtc cctccatcta
    31681 aaaatctttt tacttctgaa agcaactcaa tttctgaaga aaccactttt ccgttcaatt
    31741 tgatcaaacc tattgtatat aagttcaggt agtgaaattg attttcagct gtttcttgta
    31801 gtgcagcgat ttttttcggg tgggttccgc cttgctgtcg agtatcagta tataagccga
    31861 tcactgggat gcctttcgca taagccacac cgatttccga tgctaccccg gcatcgatcg
    31921 tcagcccatc tagtaaagca atcattaaat cactagccaa cactttttct gtgtcagcaa
    31981 gagcaatcat tcgactgtct gcataagcag ttttgtcatt gattgcttca ttttcttgag
    32041 gaagatagat cgaaagctca gaagagagct ctctgatttc tttaactaac tgttggttat
    32101 ataacaaatc acttttagca aacattggtg cggcaaaata aattttcatc taaacatcat
    32161 cctttttatc aaaaataatc gctaaagtaa gcttccatgt ttggaatgat acgctctagt
    32221 gacttcagtg tttcttcatc tgtttctaac cgttcgattc ttgccaaata agaagcacgg
    32281 cgataattca tcatcaagca agtcaatgtt tggatatcca gtcgaacgga ttttcctata
    32341 ggttcattta agacacggac ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta
    32401 ttccaaggag cgacaggatc ttcaatgatg aaatgaaaag catcagctgt tccaacaaac
    32461 ggaaaccgtt gaaggaattc ttttacatca acgatccgag ccataaaata tggttcgatc
    32521 tgctctttga tctgactatc ttcaatcaaa aatgctaaag gctcattttt ataaatatct
    32581 ccatgtaccc agtagatcat ggaaaaatgt gcggaaataa agttccataa gccatttcga
    32641 gcttcttgat caagatagaa catttctttt acgtgaaata tttcttcagc tacccagtag
    32701 aacagaacac ctcttggttc atggttgcta tcgtagtaga cagcggctgt gcgctcatct
    32761 tcattttcga aacgccaata ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg
    32821 ttttgttggg caaatttgtc ataaacggaa aatacgtctg gatgatcaac tggtaatcgc
    32881 tcaaccatac cacttactgg gatttgttta ggtagctgtg tatctctgat tttaaaggaa
    32941 agtttatcag acatgatttc ccatccttta cgccgatagt aggggatact gtaaggatac
    33001 aaataagaga tccattgctt attcttgcgc atattatcca atgcaacgat aattagatct
    33061 tgcatcaatc catggttagc atattctgga taagttccaa ctccagtcac accacccatt
    33121 ttataacgtg caccgtgaat attgacttca caaggataga tagcgatttg agagatcagt
    33181 ttattttcgt ggaaccatcc gaacactttc gatacttcca aaatcggttt tttggaacgg
    33241 ataaaagctc gtttattctc aaacccgctt tcctcgatgt ctgcttcagt cacttggaac
    33301 acataggaaa gcaattcatt gaattgatcg atatgttctt gagtgaccgg ttttaattca
    33361 agattttctc taaaatcttt ttgatccatt ttttcatctc catttattag cttgataaca
    33421 gtatagcaaa attctacaaa aaaaacttct atagctgact aattaaaaac tctttaaatt
    33481 actgcttttc gttggtataa tggaaagtgc tgattatatg aaaagaggag acgctatgga
    33541 tataaagaaa atgaaagaac gccaagagca gattcgtaat ttttcgatca tcgctcatat
    33601 cgaccatggg aaatctacat tggccgatcg tattttagaa atgacgcaca ctgtgacgtc
    33661 tcgcgaaatg caagatcagc tgttagactc gatggatttg gaacgtgaac gtggaatcac
    33721 gattaaatta aatgctgtag aactcaatta cacagcgaaa aatggagaaa catatatctt
    33781 tcacttgatt gacaccccag ggcacgtcga cttcacctat gaagtctctc gaagccttgc
    33841 tgcctgtgag ggtgccgttt tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt
    33901 ggccaatgtt tatttagcgt tagacaatga tttggagatc ctgccagtca tcaataaaat
    33961 cgacctaccc gcagcagatc ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat
    34021 cgatgctagt gaagctgtat tagccagtgc caaatcaggg attgggattg aagatattct
    34081 cgagcagatc gtggaatatg ttcctgcacc aagtggagac ttagatgcac cgctaaaagc
    34141 tttgatcttt gactctattt atgatagtta ccgcggtgtt gttctaaatg tccggatcac
    34201 ggacggtatg gtcaaacccg gagacaaaat caaactgatg agtaatggca aaacatttga
    34261 tgttacagag gtcggggtct tctcgccaaa agcagttgct agagactttt tgatggtcgg
    34321 tgatgtcggt tatatcacag caagtatcaa aactgtccaa gatacacgag taggggatac
    34381 agtaacgtta gcagacaatc ctgccgctga agctttacca ggttaccgaa aaatgaatcc
    34441 gatggtttat tgtggtcttt atccaattga tacttctcgt tacaacgatt tgcgggaagc
    34501 tttagaaaaa ctgcaattga atgatgctgc tttacaattc gaaccagaaa cctctcaagc
    34561 attaggtttt gggttccgct gtggattcct cggacttctt catatggatg tcgtccaaga
    34621 acgcttggaa cgggaattca atctagaatt gatcacgact gcaccatccg ttatttacca
    34681 tgttaataaa acggatggta cgactgctac agtagataac ccggcagatt ttcctgaacc
    34741 tgtaacgatt caggatgtag aagaaccatt cgtcaaagca cagatcatgg tcccaaatga
    34801 ttttgtcggt gcagtaatgg aactttctca acgaaaacgg ggagaattca ttacgatgga
    34861 ttatttagat gattaccgag taaatgtcgt ctataatatt ccgctttctg aaattgtctt
    34921 tgactttttc gacaaattga aatcaagcac gaaaggctat gcctcattgg attatgaaat
    34981 gtctggttat caaaaaagca agttagtgaa gatggacatc ttgctgaatg gagaaaaagt
    35041 cgatgcatta agctttatcg ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt
    35101 tgaaaaactg aaaaaactaa tcccacgcca acaatttgag gtgccaatcc aagcagctat
    35161 tggacaaaaa atcgttgccc gttcagacat caaagccttg cgtaaaaacg tcttggctaa
    35221 atgttatggt ggagatgttt ctcgtaaacg taaactctta gagaaacaaa aagaagggaa
    35281 gaagcggatg aaacaaatcg gctccgtcga agttccgcaa gaagccttca tggccgttct
    35341 gaaaatggat gagcaagata gtgtgaaata acttataatc aatcgaaaaa cctattaaat
    35401 aaaccttttt gagtagatgc aacactgagg attatctgta taaaagattt taaactgtca
    35461 tttggttatc acggcgatac taaaattaac aaaattggaa tatttttctt gaagattttt
    35521 agctgtcagt atatgatgaa agcatctcat aggaggtgct tttttcatgg ttgataattc
    35581 aagtggaagg gttcttaaat ctctaagaaa agagaaaaaa ctatctcaaa aaaagttagc
    35641 tgatttagca ggtatttcac aaagtacttt agttaaatat gaaaaagggt caagaaaaat
    35701 ccctaaagac gtagataata ctctaagcaa gattctgaat atagaaactc taataaaaga
    35761 tgaggaagat aagattgaga ttctcatcgg taaactaatt gcatacagag atatgaataa
    35821 gttacttaac aaagagttag ctgataatat aggaattagt gaagtattac tcagttatgt
    35881 gttaaataga aagagaaatc cgtcaaaaga aatgcaaaaa aaaatcgata tttttctatt
    35941 aagtaatgaa aaagaaattt tgaaggagat taatcgtgat agtgagatat ttagtttatc
    36001 aaaagacgat aaaattgtta tgggaaaaag aatacgagaa gtccgaaaga accgggaaga
    36061 gacgcttgaa aaatttggta aaaactttac catctataca ggcaaaaatg ttataagtag
    36121 atgggaaaaa ggtattaata ttccagatat tgaaaaacta atgaatattg cctatcttgg
    36181 taaggtcact gttccttacc ttatgtacgg agaagattat aagaatatac ttccaaaaga
    36241 tgaaagagtt agcgatttta aaaagataaa ttcttttagt atgggattaa ggatgaggaa
    36301 aattaggaaa gattattatc tagaaaggga ggagtttgga aagcttt
//
LOCUS       DACDHL010000020        35381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDHL010000020 DACDHL010000000
VERSION     DACDHL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.020"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            236..487
                     /locus_tag="IV228_001030"
     CDS             236..487
                     /locus_tag="IV228_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP8716966.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            1029..1187
                     /locus_tag="IV228_001031"
     CDS             1029..1187
                     /locus_tag="IV228_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8716967.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            1259..2485
                     /locus_tag="IV228_001032"
     CDS             1259..2485
                     /locus_tag="IV228_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8716968.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(2574..2966)
                     /gene="rpsI"
                     /locus_tag="IV228_001033"
     CDS             complement(2574..2966)
                     /gene="rpsI"
                     /locus_tag="IV228_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8716969.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2982..3425)
                     /gene="rplM"
                     /locus_tag="IV228_001034"
     CDS             complement(2982..3425)
                     /gene="rplM"
                     /locus_tag="IV228_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8716970.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3944..4411)
                     /locus_tag="IV228_001035"
     CDS             complement(3944..4411)
                     /locus_tag="IV228_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8716971.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4531..6069)
                     /locus_tag="IV228_001036"
     CDS             complement(4531..6069)
                     /locus_tag="IV228_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8716972.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6149..6835
                     /locus_tag="IV228_001037"
     CDS             6149..6835
                     /locus_tag="IV228_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8716973.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7036..7563)
                     /gene="msrA"
                     /locus_tag="IV228_001038"
     CDS             complement(7036..7563)
                     /gene="msrA"
                     /locus_tag="IV228_001038"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8716974.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7647..8324)
                     /gene="pgmB"
                     /locus_tag="IV228_001039"
     CDS             complement(7647..8324)
                     /gene="pgmB"
                     /locus_tag="IV228_001039"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8716975.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8321..9449)
                     /locus_tag="IV228_001040"
                     /pseudo
     CDS             complement(8321..9449)
                     /locus_tag="IV228_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(9449..11632)
                     /locus_tag="IV228_001041"
     CDS             complement(9449..11632)
                     /locus_tag="IV228_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8716976.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11629..12654)
                     /locus_tag="IV228_001042"
     CDS             complement(11629..12654)
                     /locus_tag="IV228_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8716977.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12699..13526)
                     /locus_tag="IV228_001043"
     CDS             complement(12699..13526)
                     /locus_tag="IV228_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8716978.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13523..14590)
                     /locus_tag="IV228_001044"
     CDS             complement(13523..14590)
                     /locus_tag="IV228_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8716979.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14616..15452)
                     /locus_tag="IV228_001045"
     CDS             complement(14616..15452)
                     /locus_tag="IV228_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8716980.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15449..16318)
                     /locus_tag="IV228_001046"
     CDS             complement(15449..16318)
                     /locus_tag="IV228_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8716981.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16388..17671)
                     /locus_tag="IV228_001047"
     CDS             complement(16388..17671)
                     /locus_tag="IV228_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8716982.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17685..19370)
                     /locus_tag="IV228_001048"
     CDS             complement(17685..19370)
                     /locus_tag="IV228_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8716983.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19645..20670
                     /locus_tag="IV228_001049"
     CDS             19645..20670
                     /locus_tag="IV228_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8716984.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21265..24918)
                     /gene="rpoC"
                     /locus_tag="IV228_001050"
     CDS             complement(21265..24918)
                     /gene="rpoC"
                     /locus_tag="IV228_001050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8716985.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24997..28614)
                     /gene="rpoB"
                     /locus_tag="IV228_001051"
     CDS             complement(24997..28614)
                     /gene="rpoB"
                     /locus_tag="IV228_001051"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8716986.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29342..30310
                     /locus_tag="IV228_001052"
     CDS             29342..30310
                     /locus_tag="IV228_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8716987.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30328..31233
                     /locus_tag="IV228_001053"
     CDS             30328..31233
                     /locus_tag="IV228_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8716988.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31230..32036
                     /locus_tag="IV228_001054"
     CDS             31230..32036
                     /locus_tag="IV228_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8716989.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32193..33161
                     /locus_tag="IV228_001055"
     CDS             32193..33161
                     /locus_tag="IV228_001055"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8716990.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33478..34002)
                     /locus_tag="IV228_001056"
     CDS             complement(33478..34002)
                     /locus_tag="IV228_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8716991.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34289..>35381
                     /gene="scm"
                     /locus_tag="IV228_001057"
     CDS             34289..>35381
                     /gene="scm"
                     /locus_tag="IV228_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8716992.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTT"
BASE COUNT        10138 a         7472 c         6612 g        11159 t
ORIGIN      
        1 gtacaaaatc agaaatgagg aaagtattag gatggactag aaaggagttg ttgaatttgt
       61 tgcaacagat tggtcattta tcaattaaaa attaggtgaa gttttattca tcactgcagc
      121 tgctgtttcc cagagaataa aacgtctaga agaaactggt cagttactgt attatcaagc
      181 agcaattgac tacgaacaag cttgattact aaccaaagcg tttatccacc tgttgattaa
      241 atcaaatcaa aaatcaggtt tttatacgtt cataaaagca atctccagtg tactcgaatg
      301 tgactgtatc ataggtcctt attcgatgtt aatcaaaatg gggtttcctt ctaccgaatc
      361 attggacagt tttatcaatg atttatcaaa gtttggacga accaacaccc aaatcgtttt
      421 ctcgactctt gtttccttaa tggtttatca gttctggaat gtttatcatg cgcttttatt
      481 aaaatagaaa agagcttttt tcatgcgaca aagcagtgat catttattgg cacatgcaat
      541 ctttgtggat ccattttttg aaaatacgat atttagtttt gacccaaaaa tctttaaata
      601 cgtatttgat aatttatctt aacaaccaga taattttatt cagcaccgta ttatctatat
      661 tgaattctcg gaatcaagat aaattttttt ccgtttttgt ttctttttta atcttcttct
      721 tgttattaat agtgattagg atgatattcc tatattgatc tttgataatt agatataatt
      781 tttgaataac gttccctgct attcgagcta gaaatacaag tacgccatga cctagttagc
      841 gacaactact tagagatttc ttttgttctg ggacaacaaa atgccatgac aatagcagta
      901 aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct
      961 tacccaaagc ggttcaaaaa ttttaaacta ttattttagg agatgatttt ataaaatcga
     1021 aagcctactt gaataaaaaa caagcctgtg aatttctagg aattgataat aatactttag
     1081 ataagtggat tcgtgtatat aattttccag taataaaaat tgatggcgta gttcgctttg
     1141 acgtagagga tatcaaagat ttcatgaata cattcaaaaa aaattgaaat tttcttctcg
     1201 tcacatatac tataaatagc gaaatgtact gccttacttt ttaaaggagt gaggaagttt
     1261 gcctacaaag ctttcaaatg ggaaatataa aactaatctg aggtatccaa aaaaattcag
     1321 agaaatcact ggaattgcat cagagaagta tcaaaaagta tttccaacta ggcaattagc
     1381 tattaaggcc gaaagggaaa tgaaaaagaa gattgaaaca gttttaagag aggagaatgc
     1441 aaactcacta gaattgaaag gaaaaataaa atttaaagat ttttatgaat caaaatggct
     1501 tcctagatat gaactaggtc aaactactag gtcaaacagg gctccaagta atgtaacaat
     1561 tagtaatact aaggatatat tccgattgca catccttcca atgtttggtg aatatgcaat
     1621 gaattatctg aattcaaata ctgagataat atcagatgaa ttaacaaaga aatcgaaaga
     1681 atacgcaaat ataaaaatta tcaaaggcta tgttcgctct atgtttgata ttgccgaaat
     1741 actatgttat atcgaattca accgaactac caaaattatt caatgtatta ccgcgcctaa
     1801 gaaaatggct ttagaagaaa aaaggataag ggaagggaat caagcattat catcagaaga
     1861 gctgactgat tggatagaag cagtaaacaa tgacttaaat aatcacttgc ttacacttca
     1921 cgattacaca cttttcatgc ttaccttata tctaggtgat agaaaaagtg agacttatgc
     1981 tctgcaatgg aaatatattg attttaataa aaatacggtt cgtctcaaat atgctttaga
     2041 taaatatcaa agaaagacac atacaaaagg ttggaaagac accgttattc aggcaccaga
     2101 agttgtgatg acattattaa gagaatggaa atcagctcag gctgaacaat tgttgaaact
     2161 aaaaattata caaacacctg accagtacct ctttacctat tccaaacctt ctggagaagt
     2221 aaattgtcct gttcacgctg actacttaaa ctatcgaatc aatgcaataa aaaaaaggca
     2281 tcctgaattg gctcaattat ctccacacaa acttcgtcat acctatgcaa ctatagctag
     2341 ggaaggtgga gcaaatatga atcaaatctc taatgcttta acgcactcgg atatttctac
     2401 tacaaaaata tacgtgaaca cacctgacgt agttgataaa tctgtttttg aagcatttca
     2461 aaaaggattg aataagtgtg attaacatgt gttctcaaat gtgttcttat tataaaaaaa
     2521 gacactttcc aaataattga aaagtgtcct gaaatattga tataaaaaga atattaacgt
     2581 tttgagaatt gtgaagcttt acgagctttt ttaagacctg gttttttacg ttcgaccata
     2641 cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact
     2701 tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg
     2761 ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga
     2821 ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca
     2881 gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct
     2941 gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg
     3001 tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat
     3061 tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg
     3121 atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct
     3181 gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact
     3241 ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt
     3301 ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca
     3361 gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta
     3421 cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg
     3481 gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa
     3541 cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat
     3601 aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca
     3661 caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
     3721 atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg
     3781 caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa
     3841 aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
     3901 ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg
     3961 gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt
     4021 cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt
     4081 ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag
     4141 tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg
     4201 tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt
     4261 tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca
     4321 tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca actaattgat
     4381 ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac
     4441 ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt
     4501 ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg
     4561 cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg
     4621 atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc
     4681 tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat
     4741 cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac
     4801 atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc
     4861 tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc
     4921 ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact
     4981 gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat
     5041 cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt
     5101 ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc
     5161 aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac
     5221 ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc
     5281 tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt
     5341 agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc
     5401 tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa
     5461 ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat
     5521 atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct
     5581 ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg
     5641 gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt
     5701 ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc
     5761 tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat
     5821 caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg
     5881 tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc
     5941 tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg
     6001 ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga
     6061 aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt
     6121 atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat
     6181 cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc
     6241 tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct
     6301 ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta
     6361 tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc
     6421 aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt
     6481 attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt
     6541 ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg
     6601 tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc
     6661 ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt
     6721 tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc
     6781 tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa
     6841 ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg
     6901 tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga
     6961 ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt
     7021 agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc
     7081 agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta
     7141 atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa
     7201 tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc
     7261 attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc
     7321 agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg
     7381 atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata
     7441 agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg
     7501 ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt
     7561 catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt
     7621 gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa
     7681 aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga
     7741 tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca
     7801 gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct
     7861 ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca
     7921 tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat
     7981 cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc
     8041 aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca
     8101 gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat
     8161 cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat
     8221 ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct
     8281 acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa
     8341 aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg
     8401 cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa
     8461 tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag
     8521 gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc
     8581 catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt atcgaactcc
     8641 agtaaatcca atacgtagtc gatgccagag actattttcc ctgaagcaaa tgctaacagt
     8701 cctgcaccca ttccaccacc agcactactg cccgatgcct cgattacttg aggggcaaat
     8761 ctcatataaa aatcatgcat ggcttggtca gccactgcta attcattttc tttcaagcct
     8821 ttttgtggtc cgaaaacaaa agaagcgcct tcttttccgc ataaaagatt agtaacatca
     8881 gtaatgacag tgattttggc tttaatcagc tcaataggca cgttcgtttt atcaactgta
     8941 gcaattttac ctagattttc tcccagtgct tctacttttt gattatcttg atcgtaaaaa
     9001 gcataaccta gaccttctgc catgccgatg ccgccatcgt tggtactact tccgccaacg
     9061 ccgatgatga tgtgtcgaac accttgttgc aacaattgtc gtatcagttc tcctactcct
     9121 cgcgtggata acctaagtgg ctgttgttgg tttagtggga catctttcaa tccgacgatt
     9181 tctgccattt caaaaatcgc agtttgaccg tctgaagcgt actggatttc tttgtttttg
     9241 ccaaatgcat tagtaacgac tgctgtttgc cgcttcaacc ttaaactgtc tgctaatgat
     9301 gaaaccgttc cttctcctcc atcgcctata ggtagcagat catattcagc ctgaacaaaa
     9361 acttcttgaa aacctgattg aatggcttgt gctgcctcta aggctgtcag actttctttg
     9421 aaagaatctg gcgcaatcag aatacgcatt attttcccct ccgttccaag atcagttctt
     9481 tttccagctg gtattggtct ccattgacaa aaataggtaa tgtttctttc gtctcttttt
     9541 ttattactat ttgttttttt gtcaaaacaa atagcaacct gtgatttttc caaataaaag
     9601 gaaaagaaat tttttcccat gctgaaggaa gtcttggaga aatagaaaga gttccattcg
     9661 ttactgtaat acctgaaaaa ccaaatataa ctgctagcca tagtgctcct aatgaagcag
     9721 catgaattcc gtcatctgat gaatgcggat ctggtcctaa atcaatcagg cacgcttctt
     9781 ggaataaatg atacgcccat tccatttctt ggatacggca agcctctatg gcgtgaatcg
     9841 ctttactcaa cgatgagtcg tggattgttt tgttttcata gtaatgcaaa ttcttagaaa
     9901 cgattggttg agaaaaatac tcaggtaata agtataacag catgacaaga tctgcttgct
     9961 tgctgacttg gagttcattt acttcgctac gggcataatc aagtaaaata gactggctgc
    10021 caggacgttc tttgtaagtg gtcaaatcaa taactggctt actgagatac gtgtcatctt
    10081 gcggaatcag ctgttctttg ttaggcactg gcaaatataa cttatctaaa aaattttgac
    10141 attctttcaa aaaaagcgga tcgccagctt gatatttaac agctaaggcc acattataat
    10201 gcgccagata gttcgtatat gcattattgt cgatatattc tgtgtattca tctggaccaa
    10261 tgactctgtg tatctctaaa tgatcatcga cttttacagc tctgctcagc caaaaagctg
    10321 ctgtttcatg aatgagcgtt tttccctttg tttctaaaaa cgattgatcc tctgttgctt
    10381 ggacatactc ttgaacggca taagcaaggt ctgcactgat atgatgttct gcggcagctg
    10441 aagcaacttc ttgtcttgtt ccggttttga tattcaatgc agcaaatttg ggtgtctctt
    10501 cctgaccagt gtgtgcgctt tcccatggga aaagtgcacc ttgatagtga taatctcttg
    10561 ctttttgccg tgcgccttct aatcgatcat aacggtacag cagcaaattc tttgctactt
    10621 caggaaactg atataaaaag aaaggcagga taaaaatttc cgtatcccag aaaacatgcc
    10681 ctttataacc ttcgccagtt agtcccttcg cccctacact aacacttgga tcattgcggg
    10741 gagtcataat ctgtaaatgg taagcagcga agtccatcgc taactgatcg aaagaagaat
    10801 cgccttggat cgttatccga tgctgatgcc aaaaatctgt ccaataagac atcgactgct
    10861 ctaaaatttc tgtataacta acgcttttct gggcttgggc aatacttttc ttaaacgggt
    10921 ctgtttgctg aagagaggta gagagcacga tgatcttctc cagcgtaaag gacgtatttt
    10981 ttctgatctt ttctgaaaag gaagcggtga tccggcgatt ttttgcttgg aatacacctg
    11041 ctttattgaa tttagctgca atcccaatat caattttact ttctgttgtc tggtaggatg
    11101 cactgatcaa atcgtcacca aatactcgca gttctttttc aaccaactgc tgtgtaccaa
    11161 aattggtttg ctgtccatcg atacccgttt tcacatctac ttggcagtct tcggtcaacg
    11221 gcttgatgac cactttcagg gctgcctgat ggagattatt ttgatcagca acacgacaag
    11281 taatgacttc cacttttttt ccttgagcag tttcaatgag cgcatagcgt ttaagctctc
    11341 ccatttctaa atctagtgcc cgctcgtatg caagaacttt catcgtctca agagaaaaat
    11401 gttcaccgtc gatttttatt tcccatctga tgacatctgg gagattcact aactcggctg
    11461 attctgctcc taaaggtcgg ttatatacac cagctgcaaa cattcccctt acttgattcc
    11521 gatagtcttc ctcctggctt cctctgatac caaaaaatcc atttccaatg gtcatctgac
    11581 tcgcatactt attgatatac tctaaatcaa actgtttttc ttctactttc ataaggtaat
    11641 aacttctcct gtttctgccg aacgatataa attttccacc agattctgga tgacatatcc
    11701 ttgccagcca tctgcaatca tctgagtctg gtcaccgagc gcccgattca caaaatgagc
    11761 catacttagt tcatgtgggt cattaactgg cgtggtcttt tgtaaggaaa ccagctttcc
    11821 atctacatct tcgaatagtt cgcctggata cagattagca cctgcctgat cgccacaaaa
    11881 agccacattc agtttgtctt ctctgatatt caaagcaaac gaagtattca gttggatgat
    11941 cttgccgttt tctaattcca gtgtgccaaa aattgcatct tctactgtga atttgcttgg
    12001 atcccatttc ccgaatttcc ctgaattttt tttcgtacca attttttgga agctgtacgc
    12061 ccgaacgctt tttactttag gaaatccagt aatatacatc gctgtatcaa gcatgtgaat
    12121 gccataatcg atcaaaggcc cgcctccttg aagttctttg tcgataaacg ttccccagcc
    12181 tggaacacca gaccgacgaa gcgcattcgc ttctacgtaa taaatatccc cgagaacatc
    12241 aagatgattc ttgatcattg ttgcttcgtc agaaaaacga tgctggaatc cataagccaa
    12301 aatctgcccg tttttatcgg ctgtctctgc catctttctt gcttgatcag ctgtcatagc
    12361 tggtggtttc tcgcacatca ctgaagcatt agcttcgagg gcagccaata catgttcgaa
    12421 gtgaaagcga ttggctgtac agacactcac gatatcggga gaaatctcat tcagcatttg
    12481 tgtaactgat gtatacgcat gagaaaatcc tgtacgtcgg caaaagtctt ctgctcgctg
    12541 gctgactgga tcaaccacag cggccaattc taagacatct gatctttttt gataataagc
    12601 agcatgtact ttttctgcta cttgtccagc gcctatgatt gctactttct tcatttattt
    12661 ctctctcctt tgcttattta cttattgtta ttctttctct attctttctc taaatcattt
    12721 aaatgacgcg ctgtttccag acagtttttc aagtattgca atgattcctg ataaattttc
    12781 acttcatttt ctcctcgcaa acgacattca tataccatat aaccttcata ctcattttga
    12841 agtagcgttt gaaaatggcg agtaaaatca attgcgccag atcctggttg gtagcgatga
    12901 ttatctgcta aatggatatg acccacatac tctttttgcg tagtcaatgc ttttgaaata
    12961 tcatcttctt caatattcat atgataaaaa tcagcgatga tctgcacatg tttcaattga
    13021 ttagagtcaa tgtaatgacg cgcatccgct aacaaattga tcatatggtc ttggtatcgg
    13081 ttcaaaggct ccaaataaat aaccgtacct gtttcttcgg ctactttatc taaataacga
    13141 agtgattcgc tgactgcttt atagtctcct tctttgcttc ggggtgaaac catcggcggt
    13201 aatcgataag tgaacattcc ccaagctgct gggataacga ttcctttgcc tccaacctcg
    13261 cttagtgcac gcaaaatctc agcaatttga cgtacaccat taagacgccg ttcttcgata
    13321 aagtcaccga tccacccatc gtagccgtta caagctgtca ctactgggat ccctgtttct
    13381 ttgattgcct gtttgactgt gtcgatattt tctattaaca acttaccgtc gatttcgtat
    13441 tcttcaaatc cacagtcttt gataaatcta aatttttcta aaatgtctct tgggaaaaat
    13501 gcttgatctt gcgtaccgaa tttcattgtt ctcctcctcg gacttgaacg cccattttga
    13561 tactttcttc tgggtgctgg tctacataac gcatgtaagc ttctgcgctt tctgcaaatg
    13621 gcacaactgg atcaatgatt tcttcacaat ccaagtaacc gttcatcaat aattcccaac
    13681 atgtgtcctc gattcgttta cggttccatc ttgggtaatc tgggtttggt tcactagatg
    13741 ctcgagcaaa gacgattttt gcattattga aatgtgcttc gcgaccaaga ttaaacccag
    13801 ctggaaaagg tttagcaaag gcaacataag aaatgatccc gccatagccg atccctctta
    13861 aagaagcttg taatgcggca gcactgccac tcgtttcaat aactgaatct actccacgtt
    13921 tatctgtcaa ttcctttaga cgaaaaccga catctgcttt cataggatct agcgtgtaat
    13981 cggcaccgta tttttctgct aattctctac gctgcgctat gggatcgatc ccaacgatgg
    14041 tagtagcacc tgctttttta gctaattgaa tcgcaatttg cccgatagcg cctaaaccga
    14101 agattgctac agcatcacct gctcgaacat gtgcttcccg gataccacct aaagcgaatt
    14161 gagcaggatc gtaacatact gcatttttcc attgatccaa ccgatcaagt ttacgtagtt
    14221 tataattatc aacaccgttt gtaatcagcg tttcacgaat gggtccataa gtgcagacta
    14281 actctccttt ttgatactct gtcaccaaac tgcccgtttc ttcgatcgta ccgacaatca
    14341 tatttcctaa ttgaaattcg ccaaatacca caccgcgttg gcttccttct tctcttggca
    14401 agaataggcg ccattcttct gaaaattctt catcgataaa tggacttaat cctctaaagt
    14461 cgacaatttc tgttcctggt tttggagtag cgaaaattac tcggatacgt acttcgttct
    14521 tttcgatctc acggtcttcg taatctttga atccggctat tcttggttct aatgcaacta
    14581 attttttcat cttttctttc ctctttcttt tctttttatc cttttacgcc gccttcgact
    14641 aaatcacctt taataaatcg ttcagataac gcatacataa tgatgactgg taaagcggtc
    14701 accaatgaag cggccatcat tcgtccccaa atgtagtctg gtgtactgaa taatgtgttc
    14761 aaaccgattg gtaaagtaaa tttttcggag gtcgacagga aaatcgaagc aaataaataa
    14821 tcattccacg aaaccataaa gcaataaacg aagacagaaa tgattccaga aatagccaat
    14881 ggtataatga tccgaataat gatctggatc cggctaagac catcgatcat tgctgcttct
    14941 tcaatatcag tcggaatcgt gtcaaaataa ctttttagca tgaatatcgc agtaggaagt
    15001 gtttgaacga ccatcgtgat aatcagtgct gtattggtat catacaaacc tagaccggaa
    15061 atgattttaa ataatggaac aacgatcaaa ataccggaaa acatataaac ggtgtaaaag
    15121 ctagcattga ttttcgagcg agctttgaag cgtagttttg acaaagcgta tgccccgctg
    15181 atcccaatca ctactgcagt tacggaggct atcaaggaga tcactaaact atttttgaaa
    15241 tacatgatga atggaaaaat ctcagcattg aaaatatcct gataatgttc aaaagtccat
    15301 tctttcggaa aaatagtttg tgtcgttgca attgcttctt gtgaatcctt gaatgatgtc
    15361 atcagcatga tcaaaaatgg aaacaaattg acagctaata gcaacaaaat cgtcgcgtaa
    15421 aataagattt tcctgctctt tttatttttc atttcaagtc caccttcttt cgtgagagca
    15481 agacgaccgc tgcaataatg atgaacaata gaatcgatat agctgctgct tttccaagat
    15541 cattgaacgc aaaggctgtc tgatacaaat aaacgcctaa gatattgact ttattcgtca
    15601 gtagatagac atctgtaaac atatagaaca tccagataga tcgtaaaaca atcacagttg
    15661 ctagtactgg tttgattgcc ggcagtgtca cgatcttgaa cttctgccaa gcatttgcac
    15721 cgtccatttc agctgcttca taaagcgttt tgtcgactgt ttgcaaaata gccaaaaatg
    15781 aaataaacgc atacggatag tatctccaga tagcaaacaa aacgaccata aagaagctag
    15841 caactggctg gtcgaaccac agcggtgctt cgttgaaaag ctgcaaccta tctactaaca
    15901 gatcattgac gattccataa ctattattga acatatattt ccaagcaaaa atcaaagaaa
    15961 tagatggcgt aacataactt aataaaatca gtgaacgcac cgttttccta aatctgaact
    16021 tgcggttaag caaaatagct acggctaatc ctaaagctgt actgcctgct acaactaaga
    16081 atgtgtaagc aactgttaaa cctaaagaac gataaaattc actatcactt aaaatttcaa
    16141 gataattgct taatccgata aaaacggcgt ctgagttagg attcagcggc atatcgaaaa
    16201 aactgatctg aatattggaa agcattggaa tcagcactaa aacagctaat aaaagcatgc
    16261 ttggtgctaa aagtcccatg gctaatttta agtcagaatg tttccttgga cgattcattg
    16321 aaacctcccc ctgataatct gccaatagct atctactttt ttcgaacacc attggcagaa
    16381 cttcttgtta tttcgtattt ttttgtgcgt cagctaaagc ttgatcgaca tcttcgtttc
    16441 cgacagttac ttggttcact gcttttccga tcacacctga gcttgtgata tctcccattt
    16501 tcgtgaaatt cttttccccg actagtccaa agacttgtac ttcatcaaaa gcactagcaa
    16561 tctcagatgg cagctcgcca aaagacttga tgacttcatt ttctttgtaa ccatccagtt
    16621 cgactacttg tttattcact ggttgagcac caccaggaga catcaagacc cacttttcca
    16681 tgtttttcgg ttgagataag aaagctgtga atgcttcagc agcctctttt tgtttctcgt
    16741 ctagaccaga agaaatcgtc aatccagata ctgtgccata gacagcttga tctttttgtg
    16801 ttgggatagc aaaaccgata ttttctgagt tccccgcttc ataaacagaa ggcaaaatgt
    16861 aagttgaata gattgccatt ggtgctgttc cattcatgaa agcatctttg atttctgtca
    16921 catcattaga gcctggcatc gtgtattggg ataagttttg ataaaacgac aatgcttctc
    16981 tcatcttttc cgtatcgatc gttacctcgc cttcatcatc caaaacattt gctccatttg
    17041 acaaagcaaa ttgagagaac gcttgctcac tcattgttcc ttcagcagta ggcatagcaa
    17101 ttccatattg tttattctct ttatctgtga aatgttgagc aatttttaaa atatcatccc
    17161 aattttctgg ttctgaaaaa ccagcttctg ataatttttc tttgtcatac caaatacctt
    17221 gaacccagcc gctaattggt gcagcaatat agctttttcc atcttctgaa cgaacaaggt
    17281 ttttggctcc gtcataataa ttatcttctc cagcttcctc gataacgttt tggactgccg
    17341 tctgatcaat cagttcatct ttatccatca ctttagcaaa atcctgactg acttccatta
    17401 cttctggtaa tttaccggaa cgagctaacg taacgatttt tgtattgaac gctgattctt
    17461 ctaccggcac ttgcttgatt ttgatgtctg gattttcttt ttcaaaatca gcaaccagtt
    17521 cattgatcac acttaatcgt tcctgttcga cagaagagtg catgaattga atcgttgtct
    17581 gttgtgattc gttagagctt tgactttcac cacaagcagt aagaagaaat ccagtagcta
    17641 gcagacaggt agtaacaata atttttttca tttttttcca cctcttattt tattattttc
    17701 agccacatat attcatacgg ctggaatatc aactttcgtt cttgactgca atggataatt
    17761 tgtccagaaa acaggtctag gtaatctcct tttggtaaaa gataacctgc tgattgattg
    17821 gaaaaattat ggataactaa gactttctct tccttcgctt cgcgaataaa actgataaaa
    17881 gaatctgctg agtccagtaa gtaaatcaaa ctattcggat ggaaggcttt ttcttttttt
    17941 cgaatagata gcatctgggt caattcacca tatactgctg ttcttaatgg atctgtctgc
    18001 aactctttct caatatctgc taacaaaaat ttttgccgat tgatttttcg attctctcct
    18061 gttttttcta cgcctttgta atcatttcga cttcccagca cactttgaat ataaattgcc
    18121 ggcacgcctg gtaacgttac caaaaatgaa tgagcagcta aaaagcgttt gcttctccat
    18181 tccgtcgtat ctgttggaga acttaacgca tctagatacg tagcgtttat ctcatatgga
    18241 ctggtcgttc catctgggtt tttcttgtag tttactcggg caccctcttt cttcagctgt
    18301 tccactaatt caaggatcgt tttttccggc agaatacctc gtaccggatt caatccgata
    18361 ccgtcatggg aggctaaaaa attaaaaaaa gttgtttttt tcgatgcgaa atcgatggtt
    18421 tttaaccatt ctttcaactt acttgtatta cccgaacgaa tcgcttgaag ggtcaaaggt
    18481 ggtagcgaaa actgatagac catctgtgct tcattctcgc cattcccaaa ataactgata
    18541 ttatctttgt gtggcacatt tgtctcagtg atcaataccg ttcctggagc tgcttcatca
    18601 gcgattgcac ggaataattg gatgatttta tgcgtttctg gcaggtgtat acaagaagtt
    18661 cctagttctt tccataaaaa tcccacagcg tccaagcgaa taaatgaagc gcctttttct
    18721 aagtaaaaca aaagaatatc gatcattttc aataaaactt ctggattttt aaaattcaag
    18781 tctacttgat cttcgctaaa agtcgtccat acgtgttttt ctttcccgtt tttgtcaatg
    18841 aacgttgtaa gtaacgggct ggtccttggt ctagtcacgc tgcttgtatc tgtatccagt
    18901 ggcaaatcaa taaaaaaatc tttgtacgta tcattcaagt ttaaatattc ctgaaaccaa
    18961 gtactttcgg cagacatatg attgcagaca aagtcgaaca ttaaccgtgt ttcgcttctc
    19021 atttcttcta tatcttccca tgtgcccaaa cggtcgttga ttgcctgata atttacaacg
    19081 gagaaaccat catcagatgt atatgggaaa aatggcaaga aatgtacaac tctaaatgta
    19141 tctgccaaat acttttgata aaatttctta aatgtacgca aagttggttg ctgctcttca
    19201 aaaaattgat ccccataagt aatcaagaaa atatcttctt cgtcccaatg cagtttgcct
    19261 gcctggcctt tcttctgctg ataaacactg actttttgtt caatcataga ggcggttttc
    19321 tcagacaaat ctgagtcgcg ataaatcgct tgtaaaagtt ttgtatacat cggattcccc
    19381 tttctttttt gggagcgttc ccaacgattt gatcatatta tggaaaacgc ttactgtcaa
    19441 tagcattttt ttaaataatt ttgatataca caaaaataca aataagaaag cttatgtatc
    19501 aataattaat agcttcttaa aaaaatatgg tctctcatga tcatgcaagc tattcctaaa
    19561 ttttcaaaat catagacaaa aaagaatgtc tattgtacta tggaattatt ctaaaacgct
    19621 cccaaaaagg aggtgcatat aaaattgaaa cctactattt atgacattgc gaaaaaagct
    19681 aatgtctcaa aatcaactgt ctccagagta ttaaataacc agccaaatat ctctgataaa
    19741 gcaaaagcaa gtgtacttca agcaatcaaa gaattgaatt atgaaccgag caaaattgca
    19801 agaggactgt caggaaatgg ttttgacgcg attctagcat tatctcatcg tacttctcat
    19861 acgacctatg gcaatccttt tttttcagat gttattcaat ccatttctgc cgtttctgaa
    19921 caaaataact tcgacttgat tctacaaacg gcaaaaacac cagaagaaga gctagaaaaa
    19981 tgcgcgaaaa agatccaaga aaaaatgatc cgaggaatta ttattttaag ctctacgatg
    20041 gatgaaacac ttctttttga attggatcaa tacaatattc ctattgtggt aatcggaaaa
    20101 gtcggaaacg actacacgaa catctactca gtggacacgg ataatttcca ggatagttac
    20161 gatttggtgg aaacactgat caagaatgga caccgtcata tcggctgtct ccatgctccg
    20221 acagaagttt atgtatccaa tgatcgtctc aatggttacc gtcaatgctt atttgatcac
    20281 cacctagata tccgtaatga atggattata gacggcggct ttcgcttaga agatagcatg
    20341 gcagcaatga atgatttaat gaagctacga gaaagaccaa ctgcgatttt ttctaccgat
    20401 gcattaaaat cattgagtat ttacaaagtg gcagaacaat ctggaaagca gatacctgat
    20461 gatctttcga tcgcaggatt cagtgatccc ttattgtcct ccctattttc gccacagttg
    20521 actcggatag acattccgac aaatcagtta ggaatcaaag ccacagaact tttattccaa
    20581 ctaatccacc accaataccc tgaagaaaaa agaatcattc tttcaaccga gattactctc
    20641 acagattcga tccaacaaca agaaatgtag aacagaatta taaaagaaca gaaacactga
    20701 ggataagtca gttactctgt tcttttttca atcactcatc cctcgttacc ccctcccggt
    20761 attttagcga atttttctta ttttccatct gttttagata gctgtggata actataatgt
    20821 ttgtgttaaa ctattttttc cacagggaag aaaaaactat ttttaaccac gttgtgtata
    20881 agtttttata gttcgttttt ttaaacacat ttacccttat ttcaatccat gttatttggg
    20941 gatacttatc tctaaaacat aaatagaata acagacatgt ctactggttt tgttttattc
    21001 gaggaattat tcaataatag ctagcaaaat attaaaaata accgctctat cttcccatca
    21061 atttattttg cattcaaaaa atgatctatc tttacttttg ttgattgact tctcttggaa
    21121 gatgatcgtt ccgatacctt aattggatac gaaccgatgt tgttaagatc ctttcccact
    21181 tttcttctag ttagacaaat tattttagac aaaccaaaaa agaggttgtg acaataattt
    21241 gtcacaacct cttttttaaa atatctattt tgtttgattg ttcattgcgt cttctgcagc
    21301 catttgcgct tcaatatctg aaatactgta gacattttcg cttgctacac cgacttcttt
    21361 tggttccatg tcacgataac gttgcatacc agtaccagct gggatgatct tacctataat
    21421 aacattttct ttcaaaccaa gaagaggatc tttcttaccg cggatcgcag catccgtcaa
    21481 gacacgagtt gtttcttgga atgatgcagc agataagaaa ctatttgttt ctaatgatgc
    21541 ttttgtgata cctagtaaga caggtcgaga agttgcagga acgcctcctg agactagcgt
    21601 attgtagttt tgatctttaa agtctgcgat gtctaataat gtacctggta agatatctgt
    21661 atcacctgga tccattacac gaactttacg cagcatttga cgaaccatta cttcgatatg
    21721 cttgtcgccg atttctaccc cttgcatacg gtaaacacgt tgtacttcac gaagcaagta
    21781 gttttcaaca gataatacat cgcgaacttg caacaattgt tttggatcga ttgatccttc
    21841 tgtcaatggt gcaccacggt ggatcatatc gccttctgct actttcatac gtgctgtata
    21901 aggaacagta tacgtacgtg tatctgtttt tcctttgatt gttacttctt tggtacgtgt
    21961 tgcaggatct tctgagatat cgatgacatc ccctgttact tctgtgatca ctgcttgccc
    22021 tttcggatta cgtgcttcaa agatttcttg gacacgagga agaccttgag tgatatcgtc
    22081 tccggcaacc ccacctgtat ggaatgtacg catcgtcaac tgagtaccag gttcaccgat
    22141 tgattgagcg gcgatcgtac caactgcttc tccgacttca acgtcagagc cagttgcaag
    22201 gttgcgtccg taacaatgtt tgcagacacc atgttttgta ttacatgtga agacagaacg
    22261 gattgtcact gtttcgacac ctgcatcaac gatttgtttt gcgatgtctt cggaaatcaa
    22321 ttggtttcct gcaatgatca attcatttgt ttcaggatga cgtacttctt tacgggtgta
    22381 acgtcccact aaacgatctt ctaatgattc gatgatttca tttccctcgc ggatggcttg
    22441 gatttcaaga cctcggtcag taccgcaatc ttcttcacgg atgattacat cttgggccac
    22501 gtcaaccaga cgacgagtca agtaacctga atcggcagtc ttcaaggctg tatcggtcat
    22561 ccctttacga gccccgtgag ttgagataaa catttccaag acggatagtc cttcacggaa
    22621 gttcgaaatg attggcaatt ccatgattcg accattagga gcggccatca aaccacgcat
    22681 tccggcaagc tgtgtaaagt tggaaatgtt accacgagct ccagaatcag acatcatgaa
    22741 gattggattt ttcgcttcca aaccttccat cagtttttgt tggatgctgt cttttgcagc
    22801 attccaaaca gcaatgacac gttcataacg ttcgtcatct gtgatcagac cacgacggaa
    22861 ttgttttgta atagtttcaa cttgtttatg ggcattttca atgatttctt gtttttcatt
    22921 cagtacactg atgtcagcga tccctactgt gataccggca tgtgtcgaat gtttgtaccc
    22981 taagtctttc atacggtcaa gcatcttaga agtttctttt actttgaatc gtttgaagac
    23041 ttctgcgatg atgtttccta agtttttctt cttgaacggt aatactaatt cttgctcttt
    23101 gatatgtgca ggaatatcag taccagcttc aacaaagtat ttgtccggtg tttggacggt
    23161 caagttgaag tctgttggtt cattcagata agggaattca ggaggcatga tctcgttgaa
    23221 gatgatctta ccaactgttg tgatcatcgt acgttctttt tgccattcag taaatggttt
    23281 ttcacctaat gagttagggt tcacaccgat acgtgaatgt aaatgaacat atccgttgcg
    23341 ccatgcgata acggcttcgt tttgatctct gaagaccatt ccttcgcctt cgcgtccttc
    23401 ttcttccatt gtcaagtagt agttacctag aaccatatct tgagaaggag taacaactgg
    23461 tttaccatct ttagggttca agatgttttg agcagctaac atcaacatac gtgcttctgc
    23521 ttgtgcttct tcattcaatg gaacgtggac agccatttgg tctccatcga aatcggcatt
    23581 ataagcttca catacaagtg gatgcaaacg gattgcgcgt ccttgtacta aaactggttc
    23641 gaatgcttgg ataccaagtc ggtgaagcgt aggtgcccgg ttcagcaata ctgggtgttc
    23701 cttgattacg tcttctaaaa catcccatac ttcgtcttct tgacgttcga ttttacgttt
    23761 cgcatttttg atgtttgaag ctagttcgcg ttgaacaagc tcacgcatca cgaatggttt
    23821 gaataattca atggccattt cttttggaag tccacattgg tacatcttca agaaaggtcc
    23881 aacaacgata acggaacgac ctgagtagtc gacacgttta cctagcaagt tttgacggaa
    23941 acgaccttgt ttccctttca acatatgaga aagagatttc aatgggcggt tacctggtcc
    24001 agtaactgga cgtccgcgac gaccgttatc gatcaatgca tctactgctt cttgcagcat
    24061 acgtttttca ttttgaacga tgattccagg tgcattcaag tctaacaaac gtttcaaacg
    24121 gttattacgg ttgattacac gacggtaaag atcattcaag tcacttgttg caaaacgtcc
    24181 accttctaat tgaaccattg ggcgtaaatc tggtggaata acagggataa cgtccataac
    24241 catccagctt ggttgatttc ctgaagcgcg gaatgcttct aaaatatcca aacggcggat
    24301 tgcacgtgta cgtttttgtc cttgtgcaga tttcaattct tctttcaatt cggctgcttc
    24361 tgcatctaga tcaacattat caagtaattg tttaatcgct tctgcaccca tagcagcttg
    24421 gaattcttgt ccatattgtt cacgtttttc gcgatattcg cgttctgtca acaattgttt
    24481 tttctctaaa ttcgtatttc ctggttcgat gactacatat gaagcaaaat aaatgatttc
    24541 ttccaaagcg cgtgggctca tgtctaatac aagacccata cgtgaaggaa tccctttaaa
    24601 gtaccagata tgtgaaacag gagctgctaa ttcaatatgt cccatgcgtt cacggcgaac
    24661 tttcgaacga gtaacttcca ctccgcatcg atcacaaacg attcctttat aacggatacg
    24721 tttgtatttt ccgcaggcac attcccagtc ttttgtagga ccaaaaatcc gctcacagaa
    24781 caacccttcg cgttcaggtt ttaaggtacg atagttaatc gtctcaggtt tttttacttc
    24841 accgtaagac cagcttctga ttttttcggg agaagccaaa cctatttgca tactttcgaa
    24901 tttatttaca tcgatcaaaa ggggttccct ccatttcatt taatggcaaa ccgcaagacg
    24961 gctgatcttc gaccgccttg cagctcatag accggattag tctaaattat cttctgcggt
    25021 ttcaaagttt tggatgacat cgtctttttc atcttccact tgttcagcag ctttcttttc
    25081 taattctttg gcagtttgct gttcggcaaa ttttgtcaaa gcatcaacag tgatcaaatc
    25141 gtcgtcttca tcgtccatgt cgcgaagttc gatctctgtt tcttcgatat ccaagacacg
    25201 catgtccaga cctagtgatt gcaattcttt caccaatacg cggaatgatt ccggtacacc
    25261 tggttttgga attggttcac ccttgacgat tgcttcgtat gttttcacac gtccgactac
    25321 gtcatctgat ttgtatgtca agatttcttg taaagtataa gccgcaccgt aagcttccag
    25381 tgcccatact tccatttcac caaaacgttg tccaccaaat tgagctttac ctcccaatgg
    25441 ttgttgtgta accaatgagt aaggtccgat tgaacgtgca tgcaatttgt catcgaccat
    25501 gtgagcaagt ttgatcatgt acatcacacc gacagacaca cgtccatcaa atggttcacc
    25561 tgtacgtccg tcgtacagaa cagttttcgc atcgctggcc ataccggctt cacgaactgt
    25621 ttcccatacg tcttcatcac ttgcaccatc aaatactggc gttgctacgt ggatacctaa
    25681 ctgacgtgca gccataccca agtgtaattc caatacttgc ccgatgttca tacgtgaagg
    25741 cacccctaaa ggattcagca tgatatcgat tggtgttcca tcaggtagga atggcatatc
    25801 ttcttccggc ataatacggg atacaacccc tttatttccg tgacgaccgg ccattttatc
    25861 cccttcatgg attttacgtt tttgaacgat ataaacacga actaacatgt ttacgcctgg
    25921 tgacaattca tctccagctt cacgagtaaa gattttcaca tcgtgaacga taccgccgcc
    25981 gccatgtggt acacgtaatg atgtatcgcg gacttcacga gctttttcgc caaagatcgc
    26041 atgcaataga cgttcttctg cagataattc tgttacccct tttggcgtga ctttccctac
    26101 aagcaaatcg ccatcttgga cttctgcacc aatacggatg atacccattt cgtctaaatc
    26161 ttttaatgcg tcttccccga cattagggat ttcgcgtgtg atttcttcag gtcctaattt
    26221 tgtatcgcgg gcttctgatt catattcttc aatatggata gaagtgtata cgtcatcttt
    26281 taccaaacga cggctcatga tgatcgcatc ttcatagttg tacccttccc aagtcatgaa
    26341 accaactaaa acgttttggc ctagagccat ttcgccttgt tccatagatg gtccgtctgc
    26401 cagagtatcc cctttttcga ctttctcacc caaatgaacg attggacgtt ggttgtagct
    26461 tgttccggag tttgaacgac ggaattttgt tacatcgtat ttatctaatg caccgttgtc
    26521 gcggcgtacg cggatttcag aagcatcaac gtattcaacg acaccatcat gtttacataa
    26581 caaagcagct cctgagtcat gcgctgattt gtattccata ccagtaccaa cccatggtgc
    26641 ttgaggattg atcaacggca ctgcttgacg ctgcatgttg gcacccatca aggcacggtt
    26701 ggagtcatcg ttttccaaga aaggaataca tgctgtcgca acggcaacta cttgtttagg
    26761 tgatacgtcc atgtagtcta ctttatcaat agatacttct aagttttcac tttgagcacg
    26821 agccataaca acgtcttgag caaatgttcc atcttcattc aatgggctgt ttgcttgggc
    26881 tacgatgtaa tggtcttcga tatcagctgt caagtagtcg atttggtcag taacacgacc
    26941 tgtttcacgg tctacacgac gataaggtgt ttcgataaat ccgaatttat ttactttcgc
    27001 ataactggac aaactattga tcaacccgat gtttgggcct tcaggagttt cgatcggaca
    27061 catacggcca tagtgggagt agtgaacgtc acgaacttca taaccggcgc ggtcacgagt
    27121 caatccgcca ggtcctaagg cgaaaagacg acgtttgtgt gtcaattcgc caagagggtt
    27181 tgtttgatcc atgaactgtg acaactgaga agaaccaaag aattctttga tacttgccac
    27241 aactggacgg atattgatca attgttgagg tgtcaatgtt tcagtatctt ggatcgacat
    27301 tctttcacga accacacgtt ccatacgtgc tagcccgata cggaattggt tttgtaaaag
    27361 ttcaccgact gaacggatac gacgatttcc taagtggtcg atatcatcga cattgccgat
    27421 accttccatc aagttcaaga aatagctcat tgaagcaatg atgtcggctg gacgaaccgt
    27481 ttttacagct gcttctggat agccgtttcc gatcacgttt acttcacgtt cagggtctct
    27541 tggtgaaaat actttgatga cttggacagt cattggatct gtgaccacac cgtcttctga
    27601 tggatagtaa gtcactgaat ttaggccatt atcaatgaaa ggagctagtg tttccatgac
    27661 ttggtgagtc agaactgttc ctttttcaac gatgatctcg cctgtttctg gatcaactaa
    27721 tgtttcagct aatgtcagat tcaatagacg tgttttcaaa tctaattttt tgtttacttt
    27781 gtaacgacca acgtttgcta agtcgtaacg ttttggatcg aagaaacgag cattcagcaa
    27841 gttacgtgag ctgtctgctg tttttggttc gcctggacgc aaacgttcgt aaacgtcttt
    27901 caaaccttct tctgttcttg agtcgcttgc gtttttatgc aagtcttttt cgactgtatt
    27961 gcgtaatgtt tcgctgtcgc cgaagatttc aaagatcgta tcatcggaac caaatcctaa
    28021 cgcacgaact aatacggtta aaggaatctt acgtgtacgg tcgatacgta cataagagat
    28081 gtcttttgca tcagtttcca tttctagcca agctccacgg ttagggataa cagtagaacc
    28141 gaatccttct tttccgtttt tgtcgacttt tccatggaaa tagacacctg gtgaacggac
    28201 taactgagaa acgattactc gctctgcccc gttgatgatg aatgttcctt gttctgtcat
    28261 taatgggaaa tcaccgaaga atacttcttg tgctttgatt tcgccagtct cgcggtttgt
    28321 caaacgcaat gttacatgta atggtgcaga gtagtttgca tcatgcgcac gagcttctgc
    28381 tactgtatac tttggttctt tcaattcata atctacaaat tccaaagata gattgccatt
    28441 gaagtcatcg attggcaaaa tgtcttcaaa catttctcta agtccctcat ctaagaacca
    28501 ttggtaagag tctgtttgaa tttcgatcaa atttggtaat tccaacactt cactgattct
    28561 tgcgaaactt ctacgttcgc gatgttttcc gtattttact acgtgtccag ccaagctctt
    28621 cacccctcta aaatgtcatt caaaaagact acccgatcaa ttgttacaat aatatttcaa
    28681 atattatttt taggtaacaa cagacatttt ttgcggcttt taggcaaaaa aaaaccaaaa
    28741 atagaacatt ctcttgaaac cttacttcgt gagcttctac ttttgtttcc ttatattaaa
    28801 gccgaaacgg acaatatttc gtttctgcct tttcttcatc agatagtttt tgcaacttca
    28861 tatactacac ttattttcta taaaagtcaa cacttcctgg cgtttcttct gctttttcgc
    28921 aacattttct taatgttttc aattttatgt tttcaattat ctgacaattc ttaattgaca
    28981 gaccttttta tctcggttat actttagaaa attcattttg tggaggaact tctatgcgaa
    29041 ccaacatcaa ccaaatcgca ccaacacaac tgaataactt ttactttagc tactttagat
    29101 aggtttgtat tcatcatgat ggatacaaac gttaaacgac agtttgtatc tatgatggct
    29161 aaagaacttt gtattgcagc aaaaagccac gtagacaaaa tgctaagtgg ctgagcagtg
    29221 aaaatacaaa gtaaaagtat caagcattct actttttcaa tttattttcc gacttttcaa
    29281 tccatttgga cattttgtct gaatggattt tttgtttccc aaaattaagg aggaaaaaga
    29341 aatgaaaaaa aatataatca tcggattagg cgctgccctg ctactagctt taggaggttg
    29401 cgcacaaaaa gacaattccg cagaggcatc agatggacaa aaaacaattg gcgtattgca
    29461 acttgtacaa cataactcgt tagatgctgc ctataaagga ttcaaagaag gattagcaga
    29521 agaagggtat aaagaaggcg ataatctaac aatcgattat caaaatgccc aaaacaatca
    29581 agacaacctg aaaagtatga gtgagaaact cgtcaaagaa gatcctgatt tgctacttgg
    29641 aatcgccact ccagcagcac aatctttatt aaatgaaaca accactatcc cgatgactgt
    29701 cacagcggtc acagacttgg aacaagccca tttagtcaca tccaatgaaa aacctggcgg
    29761 caatgtgact ggcacgacag atatggtgcc aatcgaaaaa caaatcggct tactgttaaa
    29821 tatcgtgcca gacgccaaaa caatcggcat catgtataac aacggtgaag caaactctaa
    29881 gatccaagct gatttggcag aaaaagaatt gaaaaaagcg ggcgtcaaag tcaaagtact
    29941 gacagcaaat tccacaaatg atgtccaaca agtgacgaaa agtctagcta aagatgtaga
    30001 tggaatctat attcctactg ataacacgtt cgcaagtgcg gcaagtgttg tcggtgaagt
    30061 cgcaaaagaa acaaaaacac ctgtcgttgc tggcgcagtt gatcaagtca aagacggcgg
    30121 tctagcaaca gtaggaatcg actacgaaag cttgggcaaa caaacaggga tcatggcagc
    30181 aaaaatctta gacggcaaga atccaagtga acttcctgtc gaaacagcga atgatttgga
    30241 actgtatgtc aataaagaca tggccaaagc attaggcatc aatccagaca gcatcaaaga
    30301 agctgaataa aacacaggag gattctcatg acaaatttgc tctctctttt cttatcatct
    30361 gcatcgcaag gggtcttatg ggccctactt gcgattggtg tatacgtcac tttccgtatc
    30421 ttggacattg ccgacttgac tgctgaagga agctttcctt taggtgcagg gatcgcggca
    30481 gtaagtatta caaatggcta tcatccattg acagcttgtc ttctaggctt tttagggagt
    30541 gcggcagcag ggcttgtttc cggactgctt cacacaaaat tgaaaattcc cgcccttctt
    30601 gcagggatca tcaccatgac tggtttatat tctgtcacat cccgtgtgat gggtgcgccg
    30661 aacatctctt tattgggtca gccaacgatc tttcattggg tcgagtcatt cggcataaca
    30721 aaagacaacg ctgtactgat cgtcggtctg ctttttgctt tgatcgttgt atgtttactt
    30781 gttttgttct tacgtacaga aacaggactt gctattcgtg caactggcga taatctggca
    30841 atgagcgaag caaacgggat caacacagat aatatgaaga tcatcggtta tatgatttct
    30901 aatggatgca ttgctctttc tggctccttg cttgcgcaaa acaacggctt tgctgattta
    30961 aattctggta ttggaacgat cgtaattgga cttgcttcta ttatcatcgc agaagttttg
    31021 ttacgtaacc agccactatt tctccgtctc ttgactgtcg ttttaggtgc ggtcatttat
    31081 cgttttattt tagcgttagt cttcgaactg aatgttgaac caagtgattc gaaacttgct
    31141 tctgctttcg tgctagtgat ctgtctttct ttcccgcaga tccagcaaaa attccattta
    31201 cctcaaaaaa agaaaggcgg tagtgtcaaa tgaatgacgt cttaacattg gaaaatctcc
    31261 atcatacctt tgaaaaagga acgatcaacg aaaatcatgt cctgaaaggc attaatcttt
    31321 ccttagatcc cggcgacttc gtgacgatca tcggtggaaa tggggctggt aaatctactt
    31381 tgctgaacag catcgccggt acattttctg tcgatcaggg tcaaatttta ttgaatggaa
    31441 aaaatatcac gaaaaaatct gttgtcgaac gttcgaaatc catcagtcgc gtattccaag
    31501 atcctaaact agggacagcc gttcgtttaa cggtagaaga aaatcttgct cttgctatga
    31561 aacgaggaaa aaaacgcggc ttcttccgtg gtgtcaaacc acaagaccga acatttttca
    31621 aagaccagct ggttcggcta aatttggggt tggaaaatcg tttatcctca gaaatcggtt
    31681 tgctgtctgg aggacaacgt caagctatta cgttattgat ggccacatta cagcgtccag
    31741 aactgatctt attagatgaa cacactgcag cgcttgaccc tgctacttcc aaaacggtga
    31801 tggaattgac agaaaagctg atcactgagc agaatctcac tgcttttatg gtcactcatg
    31861 acatggaaga cgcgatccgc tatgggagtc gactgatcat gctgcatcaa ggaaggatcg
    31921 tcgtagatat ccagcaagag gaaaaacagc agctcacagt gccccaatta ttagcgttgt
    31981 tcaaacaaaa cagcggcacg gcattaaaag acgatcaagt acttttatct tcataatcag
    32041 aaagaggatg ggacaaaaaa tgaacgccca ccctcttctt ttttctcttt ccttctttcg
    32101 tttttgcttt atgtatcaac cgttgtttct tcgaaaacta ttttgttctc tccttcttcc
    32161 tgatagaatg gatgggaaag gagcttgatg ccatgacaac gaaagacaag gtacttcaat
    32221 tattgaaaaa gacaccagat tttctttccg gagaaaagtt ggcacagcaa ctggaagttt
    32281 cccggacaag tatttggaaa gccatcaaag aattggaaaa ggcaggctat cgttttgagc
    32341 acgggcctac agggtatcgt tacctgccct ctgacgtact agatgcaaaa gaaatccagc
    32401 aagagctacg gtcgtttatt cctgatttga aagtacaaac gatgatgact tctgaatcta
    32461 cgatgaaaga tgccaaactg gcagcagctc aaggagattc tgtttcgaag ttatttgttg
    32521 ctgatacaca agaacaagct cgtggacgtt ttggacggcc ttttttcgct gaacctggac
    32581 gaggaatcta catgagtctc ttgctccatc cagacaaacg gtttgatgaa ttgcctcaat
    32641 atacgatcat cgcagccagt gcctgtgtga aagccattga atctgtaacc ggtcgttctg
    32701 cttcgatcaa atgggtcaat gatatctaca tggatggaaa aaaagtctgt gggattttat
    32761 ctgaagcagt cagtgatatg gaaagcggca ggatcagtca tatcatcatt gggatcgggc
    32821 ttaatttttc tgttcctcaa aatcattttc cagctgatct ccaagaaaaa gccatctctg
    32881 tctttcccga cggacaagct agtgtcacaa ggaatcagct aatcagcaaa atctggcaag
    32941 aattgttcac ttatctttct cacccagaaa aagcgttgga cttctataaa gaaaaatcat
    33001 tcgtattggg aaaaaccgtg tcgttcaatc aaaaaggaac gacttatgtc ggtcttgcaa
    33061 aagaaatcgg tgatcatggc gaactgatcg tccaaacaaa tgaacagaag atgagtttgt
    33121 cttctggcga aatcagtctc acatctatcc gtacgcaata attatcagat ttcacaaaag
    33181 gacacctgat ctcatacagt gtcctttttt ctgaattctc ttcaaaagac tctccttatt
    33241 ctatcagctg acagtttctc tatcctctag aaaaaggtat tctaaaaata tctataaagc
    33301 atggtacact ttctttaaat gaatcaatgc aaaaaacaag agagtcatca gtttacacga
    33361 tcaaaagaga tagcaggccg cttccttttt caatcttata gatagaccat gggtaacgat
    33421 aaaaacaaaa aagaacttgc gacttatttt gccgtaagtt cttttttagc tagtgcctta
    33481 atctcttaat cccaacaaac gtctcgcata gatcacctga ccagagtgca tagcagcatc
    33541 atcaataata gaaacgagac gcattcctcg tgtgactggt ggtgtccagt tctcgtcaat
    33601 tacttcatct agctcggaag gttccagcct tcttaagtaa gaaatagcaa aatcagcagc
    33661 tcctcgcaag tatcctagca gatcttctat ttgattagct tggatctgct ttgcttgttc
    33721 cagactatgc ttccaatctt gttcgttatc tgctatcgtc atcggaaacc ggctttccca
    33781 gtcttgacta tgccaaattg gagaagttcc tgctaaatca gcaatctgga aatctaattc
    33841 tcgagcggta tgccaagcca gccaagtcat tgatttgatc tgcggggctc tatcttccac
    33901 tggaaaacga ttggcctcct caatagagac gccttcaaac aaacgttcaa accgatcgta
    33961 tgcccgatcc acactctcgc atagcatatc taaataatcc atttattttc ctccttttta
    34021 tttatcctac ttactttttc ctaattctag gatacctact tatatgttac tacacaccct
    34081 tattttttac actttaacag tcaccatcat caatagtttg gagattgaaa gaaaaagata
    34141 gtgtcgaact atcaattgaa caagaagcaa caatatgaac gtttcattaa tattctttaa
    34201 ataaacaatg atttttcaaa cgaacagttt aagactagga gaaaaagagt ataataggaa
    34261 aatgtttgaa aggaaaggaa ggggaaaaat gaaaaaaagg gcaagtttac tagtcctagt
    34321 tgctttgttg ttcaatatct tttcgtatgg tgtggccgtt tcagcggaaa catacattcc
    34381 taaagaccct gggaatattt ataatgatct aactggtaac tatggcttgt taggaattgc
    34441 tagtcaattc cacgtatttg ctaaaaataa gacaactgtg aatgcacata cagacggaaa
    34501 tatagcaaca catgaacttg atgcaaaaaa taattttggt acagagcttc atgaaggttt
    34561 attgaaccaa gaaataaatt atatacaaaa aactgattct ttcgctaatt cttcaggcat
    34621 acctacaggt agcacaagaa tgaataaatt tgtcgttggc agtaacgtgg atgtagcact
    34681 tcaagatggt catcctacag tcaatggatc caggcttgat cacttaactc cagatgattt
    34741 ttataaagat cgtgctgggc atgaatatat tgatttcgag caggaatttg ctaaattgaa
    34801 tgtagcagca aatgatcttg caacgatcac tccagctaaa acatatactg ctgcagattt
    34861 tccagacatg aacaatcgga caatcgattt aactgattta tcagataccg ggtttttatt
    34921 ggtaaatatc gatgcagaag tgttaacgat gaatacgcca cttcaaatca ttaatccaaa
    34981 tgatcaagta gttgtattta atgtcatcaa cagttcttct gcattgaacg tacagtcacc
    35041 aatcaaatac aatgatcgtt caaatcatga aacagaagat ttttcggatg cgaatatctc
    35101 ttggaatttt ggaaatgaga tgactgattt gacaatcagt gctccttttc aaggaacaat
    35161 ccttgcgcct aatgctacta tccgcattac tcaaaaccaa gacggaacga ttataggaac
    35221 aaatgtgatt ttagatgcag ctaccaatcg ttgggatcct aacgaaatat ttatcagcga
    35281 caatggtaca gatacgacag acacaagcga cagtacagat acatctacca caagtgatag
    35341 ctcagattca tcaacttcaa gcgacagtac agatactacc t
//
LOCUS       DACDHL010000021        33563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDHL010000021 DACDHL010000000
VERSION     DACDHL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.021"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(244..678)
                     /locus_tag="IV228_001058"
     CDS             complement(244..678)
                     /locus_tag="IV228_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8716993.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            826..1967
                     /locus_tag="IV228_001059"
                     /pseudo
     CDS             826..1967
                     /locus_tag="IV228_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2093..3172)
                     /locus_tag="IV228_001060"
     CDS             complement(2093..3172)
                     /locus_tag="IV228_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8716994.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3275..3715)
                     /locus_tag="IV228_001061"
     CDS             complement(3275..3715)
                     /locus_tag="IV228_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8716995.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3772..4182)
                     /locus_tag="IV228_001062"
     CDS             complement(3772..4182)
                     /locus_tag="IV228_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8716996.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4362..4796)
                     /locus_tag="IV228_001063"
     CDS             complement(4362..4796)
                     /locus_tag="IV228_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8716997.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4896..5744)
                     /locus_tag="IV228_001064"
     CDS             complement(4896..5744)
                     /locus_tag="IV228_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8716998.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6133..7899
                     /locus_tag="IV228_001065"
     CDS             6133..7899
                     /locus_tag="IV228_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8716999.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            8001..9260
                     /locus_tag="IV228_001066"
     CDS             8001..9260
                     /locus_tag="IV228_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8717000.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9407..10708
                     /locus_tag="IV228_001067"
     CDS             9407..10708
                     /locus_tag="IV228_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8717001.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10711..11562
                     /locus_tag="IV228_001068"
     CDS             10711..11562
                     /locus_tag="IV228_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8717002.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11575..12390
                     /locus_tag="IV228_001069"
                     /pseudo
     CDS             11575..12390
                     /locus_tag="IV228_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
     gene            12541..13707
                     /locus_tag="IV228_001070"
     CDS             12541..13707
                     /locus_tag="IV228_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8717003.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13937..14584
                     /locus_tag="IV228_001071"
     CDS             13937..14584
                     /locus_tag="IV228_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8717004.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14668..15612
                     /locus_tag="IV228_001072"
     CDS             14668..15612
                     /locus_tag="IV228_001072"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8717005.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15747..17198
                     /locus_tag="IV228_001073"
     CDS             15747..17198
                     /locus_tag="IV228_001073"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8717006.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17219..18055
                     /locus_tag="IV228_001074"
     CDS             17219..18055
                     /locus_tag="IV228_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8717007.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18055..18714
                     /locus_tag="IV228_001075"
     CDS             18055..18714
                     /locus_tag="IV228_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8717008.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18840..19529
                     /locus_tag="IV228_001076"
     CDS             18840..19529
                     /locus_tag="IV228_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717009.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19535..20491
                     /locus_tag="IV228_001077"
     CDS             19535..20491
                     /locus_tag="IV228_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8717010.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20535..21047
                     /locus_tag="IV228_001078"
     CDS             20535..21047
                     /locus_tag="IV228_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8717011.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21303..21851)
                     /locus_tag="IV228_001079"
     CDS             complement(21303..21851)
                     /locus_tag="IV228_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8717012.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21940..23121)
                     /locus_tag="IV228_001080"
     CDS             complement(21940..23121)
                     /locus_tag="IV228_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8717013.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23357..24865)
                     /locus_tag="IV228_001081"
     CDS             complement(23357..24865)
                     /locus_tag="IV228_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8717014.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            25024..25215
                     /locus_tag="IV228_001082"
     CDS             25024..25215
                     /locus_tag="IV228_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717015.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25418..26335
                     /locus_tag="IV228_001083"
     CDS             25418..26335
                     /locus_tag="IV228_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8717016.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26328..27401
                     /locus_tag="IV228_001084"
     CDS             26328..27401
                     /locus_tag="IV228_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717017.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27549..28067
                     /locus_tag="IV228_001085"
     CDS             27549..28067
                     /locus_tag="IV228_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717018.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            28048..28440
                     /locus_tag="IV228_001086"
     CDS             28048..28440
                     /locus_tag="IV228_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8717019.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28516..29451
                     /locus_tag="IV228_001087"
     CDS             28516..29451
                     /locus_tag="IV228_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8717020.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29584..30363
                     /locus_tag="IV228_001088"
     CDS             29584..30363
                     /locus_tag="IV228_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8717021.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30356..30673
                     /locus_tag="IV228_001089"
     CDS             30356..30673
                     /locus_tag="IV228_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8717022.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30917..32527
                     /locus_tag="IV228_001090"
     CDS             30917..32527
                     /locus_tag="IV228_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8717023.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32684..33532
                     /locus_tag="IV228_001091"
     CDS             32684..33532
                     /locus_tag="IV228_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8717024.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        11104 a         5986 c         6513 g         9960 t
ORIGIN      
        1 tttacatatt tttaatagaa aatataattt atctatcata ccgctattta gtcggtcaag
       61 tacttctttt ttaaaaaaag tattcatcat aaaagccact acttgcggat cttcatcata
      121 aaattctgta gcagtacttg aaaagatgtt ccaataaata tctaatgctg catattgaat
      181 ttgtacctct tttccagaaa aagactttct gccattatta gaaaaggaga tagacaaacc
      241 gtactatgct aaaaatgcat taatcttttt cattttcgta tacaaataag aggcactata
      301 gtttgtttgc tgactgaaac tgataatcga atttgtctga cagctgaaca aagaagtcaa
      361 gatctgataa ctgactgatt cgttgcagta tcttccaaat agacgataat agcagtaagc
      421 cggatcaatt ccttcaaaat tcaactttaa aaaaggtttt tgcttatcaa tatgcagttc
      481 tgttagattg agacgctcaa agtcttgttc taattctttg atgtatagta aaaaatttga
      541 ttcagacata tggaaatgat ctgctaaata ttgtgtagta ataaagaaac gatgttcaga
      601 taggaaaaga aaaatatcta attttgtatt gctttttttc ttcaagataa acgttttaat
      661 cgcttcttga gtttgcatga aagaggctcc tttttactta aattgttaaa acttgaaaat
      721 attactgttt ttttcataaa tttagacaaa aaaacagtaa acatataggt agaatctcta
      781 ctatcttatt tcttgcatac aaaaaaatca aggagtgaga gagttatgag atattcaacc
      841 atcttaatat caacatttgc agccttaagc ttatcgctta ctataaatca gacagtccac
      901 acagaggaga cagaaacagc caaatggaca gaaaccaagt gggccaacgt caatcgaatt
      961 acctttcagg atgaattttc atccttcaat tcaggagtcg caaaattaag aaaaggacaa
     1021 acagcgacca tcaaagcaaa aattgactat tcgggagact tagcacctat actacaatca
     1081 gctgttacat ttgagaatgt tgatcccact ttgtcgattg gctatgacga gaacactttg
     1141 actatccaac acaagcaagc gatatttacc ttcaccgtta cattaaatca ggacttgtta
     1201 aaaccagcgc tttttactgt caaggtatca gatggactac cgaacgatca tcatttaacg
     1261 ccgtatagtc agcggcagac aattgagcaa gattcagcca tcgactcagg aggagatctt
     1321 gttgaagaac caacagataa accagaaaat gaaaataagc cagaagtacc gcctacagaa
     1381 aatccagacg gagaacagaa accggaaata gaacctggag aagaacccga tacagaaact
     1441 caaccagagc cagacaataa atcaaaacct gaaataaccc ctggagaaaa accagatgta
     1501 actccagaac cagatacgga ttctggaaac caaacagtac cggaaacaaa tccagacaca
     1561 gataatgaaa ctgaaaatcc agaaaaacca gaggtagatc cagaagaaaa accagatgtg
     1621 actccagaac cagatacgga cgcaagagac caaggaatac cagaaaaaat aaacaaaaaa
     1681 acaattcaag aggatggaaa aaaagagagt aaaaaatcaa atctagcaat tctaaaaata
     1741 aatgaagagc agctgaataa aaaatcacga atatttgata gcgcccaatc agctgaaacc
     1801 ttaaaaagta gcaaggatac aacctttgct gtcctgaaac gaaaaataaa cagctaccaa
     1861 aatccggaga gtcacaaaac aaagttatac tgtggagcgg aatcattcta ttaagtatag
     1921 caacaatgct ttctgcaaaa agatttaaac aaaacagatc tctctaacat ttgaataatg
     1981 tctgtgaacg catttttcaa ataaaaagaa atatccagaa aatgacccca aaataccaga
     2041 ttgaaatctg atatttgggg tcgttcatac tttatctttc ttcatattta ttttaaggga
     2101 tatctgtttc ttttggaaga tggttcggga tctttttcac tttcaataca tctaaaaaga
     2161 aggtaaagat cggaatgccg acaatcaagc cccaaacgcc aaagaaccgt tcgctaacaa
     2221 gtaagaccac aaatgtataa aaaatcggca gttctgtttt gctggacatc aacttcggat
     2281 tcagcacata agactcaatt agatggacaa tcaaaatcgt aatcaggata taaacaacat
     2341 ctcggatgcc tccttgggaa taagctatga aagatagagg aatacaagaa atgataacac
     2401 ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc taaactcagc agctgatgaa
     2461 agccaaatac agccaaggcg attgtagtga gcaacgtatt taaaagagcg acaacaaatt
     2521 gagcttctaa taccaaacca aatgtattga caaacttatc ggcaaaatag taaatatctt
     2581 gaaagaacca agaaaattca ctttttaaaa acaatcttga gaatacaatt gtttttttct
     2641 tttcaatcat aaaaaagaaa cttaagatga atgacatagc aaacgccact gccatttttc
     2701 ctatatcctg taaatagcca agcgcaatcg aagcgccatt ttgaagctgt tcaaaaaaat
     2761 cgtttcgctc aatataagta tggatatatt gtaaaacagg atctgcatct tttggctgat
     2821 gctcgtaaaa attaataacg gactggatca tgtcaaatgt ttgatgaata agcatcggga
     2881 tatactttgt gaatgccaga tagataaggt aaacgatcag tgtataaatc atgatagcta
     2941 aaacacctgt agggattcgg acaaatcgct gaatgatttg aacaagatga atcatcaaat
     3001 aagtaaagat aaatgtgagc aaaatggttg taatcatact tctaactaag aaaagaacaa
     3061 aaacgacaaa taataaaaca gtgagacggc gtattttttc attcgccaaa aaacgctgat
     3121 aaacggtcaa atcgacaacc tccaaataac aaaaaataaa cacgttattc attatatgat
     3181 ggaatatgaa aataagaaag agcaaaaaga aaaaccgcta aatgacgtta tcttatgagt
     3241 tggttttcct cataaaatcg ttcatctagc agtttcagcc ctttttctgg tcttgatgaa
     3301 aattatctag catttttcgg gtcagatgtt gcagtgtatc caattcttcc tgttcaagtg
     3361 gaagataaga cttgatcttt tgtggaatac cttctgcttg tttttgcaac gcagcacctt
     3421 gagcagtcag cgtaacattt acgattcttt catcacccgt ttcacgctga cgattaatat
     3481 aatttttttc tgctaacttt tttagtaagg gagtcagcgt accagaatcc agacacaaat
     3541 catttcctag ctctttcact gaagtctttt tcttttccca taaaactaac atcacaagat
     3601 actgggtata ggtaagattc agcggcttga ggaggggagt gtagtgtcta atgatttctt
     3661 tagaggcggc atataaagga aaacatactt gattaactaa atatagatct tccatttttc
     3721 cacctccgct gttttactat agtgtatcat agcggattta cacaaaaaag tctattcact
     3781 aactgctttc agagtaactt ctatatttcc agctgtagct tttgaatacg gacacacttt
     3841 atgcgctgcc tctaataatt cttgcgtttc ttccagagaa agaccttcga tagatccttc
     3901 aatctctaca cctaagacaa agctcgtttc actttctgaa taaagggaaa cttgtgcact
     3961 gacagtagaa gccccagaga tatttttttg ctgtttgatc aattctaatg cactgttaaa
     4021 acatgcactg aaaccagctg caaacaattg ttcagggttg gttgcatttt cgacgcgttt
     4081 accaggagct gcaatattca attcgaatgt acgatcggga ttatgcactt ctccagagcg
     4141 tccgcctgtg ttgatcattt tcgtagaata catttttttc ataaagttac cacctttaat
     4201 tatattttat acaattgaat tttacacaat ataattgtgg aaagcaaaga aatggattgg
     4261 cataagataa ataagagaaa gcacatgaaa cagacgcgct ttctcgaata gcaaacatat
     4321 acaaaaaata tcagagacaa aaaataatct catcataaac attacatata tttctcaaaa
     4381 agttgttttc tttcttcaaa aattacttca cgatggacct gatgaagcat ccataaataa
     4441 ccaaaaggat ctctgaacat tgagttagtc acaccaaagt ccatcatttc tgtcatttct
     4501 tgaatctcta cacagccatt tcgtaatgct ttattatgtg tgtcctcgat atcagagacg
     4561 atgatattga accagatcgt atttggatgt tcagcagtag gagcagtcaa accaagagac
     4621 gtgttttcat ccagcatatg gaatctcgta ccatacaagg taaaaatgac ttcattattg
     4681 cctctagggt aatctgttgc ctcaatcagt tgtatttcat caaagattgt ttgatacagt
     4741 tctagtgctt tcaaactatc atttaataca aaatcgattt ccgttccttt atgcataaag
     4801 atctcctctc cttttcagtc gaacagttac tagattcttt tattatacca gctaaagaaa
     4861 acgcattcaa aaaaatagcg gatttactta ccaactcatt cgattggatg ataagtaaat
     4921 ccgcgtccca ttacttctgc tacgctgctg acaatgacaa aggcacttgg atcgatttct
     4981 tctatcaatg gtttcattcg ggaaaattca tgatcagtca caacgatcaa taaaacctct
     5041 tttgactttc gattataccc gccttttgcc tctagcaggg taaggccacg tccaatctca
     5101 gcacttaagc gaaggcggat gtcttccaaa taatgttcac tcatgaccat aacagatttc
     5161 tttcgattga tacctgtttc gatataagtc atgacaattg aagtgattgc aatagataaa
     5221 atagcataaa gaaactcttc tattccaaaa ataaacagat taagagaaac aacgaatgcg
     5281 tctgtaaaaa ataagccaat cgaagtattc aagtggaaat attttttaaa aatgagggga
     5341 gggatcgtcg taccgccgct agaagagttg tttttatata aaatcgcaac tcctaaagca
     5401 aaaatcgcac ttccaaaaat gacagacaac aaccgatctg aagtaatcat tgtttctgga
     5461 actattgcaa tgattaaagg aaaaaccaag cttccgaaaa gtactttaaa aaagggtttt
     5521 ttacctaaaa atagaagagc tagtaccaac ataacgatat tcaacactaa aataactata
     5581 gctcggtcaa agcctaaagc agcttccagt aatattccta gaccgctgac acctccagca
     5641 gcaatatgat gaggccctaa aaacatattg atagcgactc ctaatatgaa gagggcaaaa
     5701 ataatcttaa tgtatgtcca tataaattgt ctcgtttctt tcatttattc acttccttaa
     5761 ttcattaata atactcttat cttattataa cttacttttc accttaagtt aactttaatt
     5821 aataaaaaaa ttaggctagt ctaaattatt atgtttcatt cttctagaat atacttctac
     5881 gccaaaaact gttaaaaaac ttttacttta gaaaattgga gaaattgatc gcttaagata
     5941 aattcaggac actaaatagt acaggaagta gactaaaact tttaaaagtt ttaagtctat
     6001 ctatttggca agtttaaaaa ataaaaattc ggtatcaaaa aactgttacc gataacattt
     6061 ttgaaagcgt tgtcttttgc ttctctatca cttatagtga tgacgtcata ttaaaacaca
     6121 aggaggaaat caatggatac agctgcaatt tatcatcgac cggaaagtga atatgcattt
     6181 ctatatacga atacaagatt tcgtattcgg cttcgtacta ggaaaaatga tatcaaaaaa
     6241 gtatatgtgc tatgtggaga tccatatacc ataacaactg aaaaatggta tcaaaaacaa
     6301 cgaccaatga aaaaatgtct aagtaccaat gttcatgatt attgggaaat tgaagtgacg
     6361 agcgaaacaa gtcgcttaca gtatgcgttc catgttgttg gggaagataa cacggactgt
     6421 ttttacggtg atcaaggaat ttttccttat cgagaaaatg tgattactga accaaatttt
     6481 tattttcgaa taccttattt tcatcaaatc gatcgattca ctattccaga atgggtaaaa
     6541 gaaacagtat ggtatcaaat ttttcccgaa agatttgcaa acggggacaa agcaaatgac
     6601 cccgaacaca ctttaccttg gggaagtaaa gatccagacc gagaagactt tttcggggga
     6661 gatttgcaag gtgtgatgga tcatttggat tacttgacgg accttggcat caatggcatc
     6721 tatttttgtc ccatatttaa agccacatcc aatcataaat acgatacgat tgattattat
     6781 gaggtggatc ccgcttttgg cgataaaaaa cttttaaaaa acttgattga tgaagcacat
     6841 aaaagaggga tacgtatcat gttcgatgct gtattcaatc atatgggtgt ccattcacca
     6901 cagtggcaag acgttctcaa aaacggagaa aaatcgatct ataaagactg gttccacatc
     6961 cgttttttcc cagtagattc ttatcagatg actgacttac cagaaactgc tgaaaatatc
     7021 ccatatgata cttttgcttt tactccattc atgccgaaac taaatacagc aaatccagaa
     7081 gtgcaaaaat atttgctgga tatcgcaact tattggataa aagaattcga tattgatgga
     7141 tggcgtcttg atgttgctaa tgaagtcgat catcattttt ggaaaaaatt cagagaagca
     7201 gttacagaaa tcaaaccaga catctatatc cttggagaga tttggcattc ctctcaagct
     7261 tggctgcaag gggatgaatt ccatgcagtg atgaattatg catttacaga ttcgattaaa
     7321 gattattttg caaaaaagaa aatcacagct tcacaaatgg tcagtggaat gaatcatcag
     7381 caaatgcttt acagagatca ggtgaacgaa ggaacattca acttattaga ttctcatgat
     7441 actgccagga tcttgaccct ttgccaaggt aacaaagaat tgatgaagtc tgtgatggcc
     7501 tttatgtttc ttcaaaaagg gtctccttgt atctattacg ggacagaaat agggatgact
     7561 ggtgaagatg acccggattg ccgcaaatgt atgatatggg agaaggagga acaagatcta
     7621 gagctattcg gttttataaa agaacttgtt tctctaagga aacagttgtc taagattatt
     7681 tctgaaggtt ctactcagtg gctaatagta aatgatcgtg aagataagct ctatttcaca
     7741 cggaaactag agggacagat catctatgtt tactttaatc agtcaaaaga accttgggta
     7801 gtagaacagg aaaatgaggt gattcttagt caaaattgtc agttgttaga agacgggaaa
     7861 gcagagatac gacaatacgg atttttgatc atgtgttgaa aactgttacc ggtaacaaca
     7921 agaaagcgtt tgcaaatgaa gatttgtgcc cattataata agggtgtaac agcacgttga
     7981 atacaaagga ggaaatgaag atgaaaaaaa gaagtgtgaa acagttgggt ttagggttaa
     8041 tctctttagg aatcttaggc ggattagcgg cttgtggcaa tggtaactct tcagcagaag
     8101 gggaagccga tgaaggaaaa acgttaacag tttccgttga ttctggatat aaagattata
     8161 taaacgaaat aaaaaacact ttcgaaaaag aaaacgatgt gaaaatcaag ctagtagaaa
     8221 aggacatgtt cgatcagttg gaatcattag cgttagatgg gcctgctgga aagggacctg
     8281 acgttatgat ggcagcatat gaccggatcg gtgcgttagg acagcaagga catctggcgg
     8341 aagtaaaact gggaaatgaa gcagcttacg atgaaacaga caaagcccaa gtgacttatg
     8401 atggaaaaat ctacggagaa cctgcagtaa tcgagaccct tgttttgtac tataacaaag
     8461 atttagtcga tacagcacca tcaacgttta aagatttgga gaatctatcc aaagacagtc
     8521 gttttgcttt tgagtcagaa gcaggaaaaa atacaggatt tttagctaaa tggactgact
     8581 tttattattc ctatggttta attgcaggtt acggtggcta tgtatttggt gatgatggca
     8641 caaatccttc agatatcggt ttgaataatg caggtgctgt agaaggaatc tcttatgcaa
     8701 cagattggtt caaaaatgtt tggccaaagg gaatgcaaga cataaaaagt gcaggagact
     8761 tcgccagcca gcaattcatg tccaataaaa ctgcagcaat cattgacggt ccatggcaag
     8821 cgcaaacata caaagaaaac aatatcaatt atggtgtagc caaaattcct actctaaata
     8881 atggacagcc ttatcaaccg tttggcggag gaaaaggatg ggtagtcagc aattatgcta
     8941 aaaacaaaga cctctctcaa aaatggcttg attatgtaac gaatcaggaa aatcaagaaa
     9001 aattctttga aatggtaaat gaaatccctg caaaccaaca agctagggaa acagcaaagg
     9061 ctaaaaacga tgaattaaca acagctgtga ttgaacaata tgaaacagct caagcgatgc
     9121 caaacatccc agaaatgggc gaagtttgga caggtgcaga aaacttgatg ttcgatgcag
     9181 catcaggttc aaaaacacca aaagaatccg cagatgaagc agtaaaaaca atcagtgaag
     9241 ccatagaaca aaaatactga ttaaaatgat aaccgtccat ttttataaaa aatggaacag
     9301 gtgcttcaga taaaagtggc aagagcgaga tggaaaatta tcttgctctt gttgccatta
     9361 tcttaaaagc agtttgcggt ttagcaagtt tagagggggc attcagatgc agcaagataa
     9421 aacaagcaat atcaacgaaa aaaaaatcac gctttgttca cttattccag ggatggggca
     9481 gtttgtaaat ggccaagctt ttaaaggtat cgtttttttc gccatattcg tcctattcat
     9541 tggtcagatg cttctgggcg gatttcatgc attggtagga ttggttactt tgggaagtat
     9601 accaatggaa gatcattcat tatttatttt gattgaaggc actttgcaat tcatcattac
     9661 aatcattttc ttgatttttt acggtttgaa tcttttagac gcttatcgag tagcgaagat
     9721 gcgtcaagag ggcaaaaaaa tcaatcggac gatgaaggaa gtcatacata atataggtga
     9781 tgaaggtttt ccttatctat tgacacttcc cgcttatctt ctcatgattt ttacgatcat
     9841 tttcccagtc ctagttacat tgttcactgc atttactaat tatgacttca atcatattcc
     9901 accagccagt ttaattgatt gggtaggggc taaaaacttt tttagtatct tctttttaag
     9961 ctcatatcgt aatacttttt ttgcagtctt ttcgtggacc gtgatttgga ctttatgtgc
    10021 aacaactttg caaatcaccc ttggtattct cacagcggtt gttgctcacc agaaatttat
    10081 taaattcaaa cgtattttcg gtatcatttt cttattacca tgggcggtcc cagcttttat
    10141 tactatcatg agtttttcga atatattcaa cgatagcgta ggtgccatca atactcaagt
    10201 gattcctttc ataaaccagc ttccttttgt agatatcagt gcaatctctt ggaaaacgga
    10261 cccgaactgg acgaaagtag caattatctt agtgcagggc tggttaggat ttccctatat
    10321 ctatgtgatg gttacaggaa tcttacaatc gatttcagaa gacttatatg aagcagcaaa
    10381 aatcgacgga gcaaatgcat ggcagcgctt ttcaaacatc acgcttcctg ctgtcttcct
    10441 agtggcagcg ccaacattta ttacacagta tacggggaat ttcaataatt tctccatgat
    10501 ctatctgttt aacaatggcg gtcccggaag cgtaggcgga ggagctggtt ctactgatat
    10561 tcttatttcc tggatctata aacttacgac tggaacttct ccgcagtatt ctatggcttc
    10621 agcaatcacg ttgattatct ccgtcatcgt tatctgtgtg tcattgctgg tatttaaaaa
    10681 gacaaatgcg ttcaatatgg gggattaaca atgaaaaaat ttcattcgag tgctcgattc
    10741 caaaaacgac tttctcactt ttttacttac ttttttcttt taatactgtc agtcattatc
    10801 atctatccgc ttttgatcac agcaagtact gccttcaagt caggaaatat tgcagctttt
    10861 agtttagatc tttcaagtga ctggacttta ggaaacttta ctcggctatt cagtgaaaca
    10921 ctttacggga cttggtataa aaatacattg atcatttctg tcctaacaat gatcgtacaa
    10981 gtgactgtag taactttggc gggctatact tatagtcgct atcgttttat cggccgcaaa
    11041 tcaagtttga tgttcttttt agtgattcag atggtaccga ctatggcagc attgactgcc
    11101 ttttatgtca tggctttctt attagaagcg ctcgataagt actggttctt gacaatgatc
    11161 tatataggcg gtggaattcc aatgaatact tggctgatga aaggatactt tgacactgta
    11221 ccaatagatt tagatgaatc tgctaaactt gatggggcag ggcatttcag gatttttttc
    11281 cagattgtat taccgcttgt caaaccgatg ctcgccgttc aagcattatg ggcttttatg
    11341 ggaccttttg gggatttcat gttagctaag ttcctgcttc gaacacctga aaatcagact
    11401 gttgctgtcg gcttacaaac atttatttct gatgtgacaa accaaagagt ctctcttttt
    11461 gctgctgggg caattttgat tgcactaccg atttgtctat tattcttctt tttacaaaag
    11521 aactttgtta ccggcctgac atctggcgga acaaaaggat aggagcagag tatcatgcaa
    11581 acatttccgt taaactattt ttccgcatta cgtacgccaa ctcaagtgtt tgcgggacgc
    11641 aaactattat catggcctaa atttttcttg atatttgtat ttttagtttc cttgatggtc
    11701 atgcctgtga ctctttttta tgccaatcag atctaagcga ttccattaga acaatttcta
    11761 tccgttcatt cgctgatcga tgaacaagga acccaaaaat tttctgaact tgaattatct
    11821 gaaactgggt tacaagcatc gcaacaaacg atagctgtga cccctgaaat tttggtaggt
    11881 gtgtctttat ctgagaaaca acaatccgat catggaactt ttattgattt tgagaaggaa
    11941 caatgggtga tccaacaaaa ggataaatct ggtatacgcc gctatacgat gaattatagc
    12001 ccatctttcc aaccagattc tgtacgtacg cccgaagatt tccaacgatt tttagaaaga
    12061 gaattttatg ccagtaatcg tccaacaatc attttatctt atagtttaag tcttggtctt
    12121 gtcctatttg tcatgacgag tctcatttta ttcggggctt cctttttctt gtggatgaca
    12181 agaaaaagcc aactttcatc cattcataca ttcaaagaga gtgcaaattt gatgctgaat
    12241 gttatgggaa taggttccat ggcagcagca gttgtagggt ttatccactt tgactttatc
    12301 ttgatgctaa gtgtccaaac aacgataact gtcttgctgc ttttatgggt ttttgtcaaa
    12361 acaagattca aagataagcg tgtcgaatga caagtttaag cgatttatgc gacaatgaat
    12421 gtgaaaaaaa tgaaaaataa agtagttttt gaaaaatttc tgaaaaaatt aaacttctta
    12481 gttgattctt ttctttttta gtattaacgt aatagggtag tttttttaag gaggaaaatc
    12541 atgttagaga aaactttata caatcaatta ttttcacgtt ctttctcgct ccctgtcgaa
    12601 gtaaattatt gggacggtac aacgaaacaa tacggagata cggacactcc tccccaaatc
    12661 aaaatcaata tccatgaaga aatcccaatg aaagagatta caagtaacgc atctttggca
    12721 ctaggagaag cctatatgga tcatcgaatc gagatcgagg gaagtatcca agcattgatc
    12781 aacgatgctt atcagcaagc aaacagcttt ttacgaggaa aagattatct aaaatggctg
    12841 ccaaagaaag aaaaacatac gaaacaaaaa aacaaagaag atatccatgc gcattatgac
    12901 ttaggtaacg acttttataa actttggcta gatccaactt tgacttattc ttgtgcttat
    12961 ttcaatacac ctgaagatac gttggaacaa gcacaaatca ataaagtcca tcatatctta
    13021 gacaaactct ttatcaaaga aggcgaaacg ttgcttgata tcggttgtgg ctggggaaca
    13081 ttgatgttca ctgctaccaa agagtataat gtcaaggcaa ctgggatcac actaagtgaa
    13141 gaacaatacg atttgattac caaaagaata aaagaagaac atttagaaga taaatgccgt
    13201 gtattattaa tggattaccg cgaactaaaa ggcgaaacgt ttgatcatat cacaagtgta
    13261 ggtatgttcg aacatgtagg ttctgaaaat cttgaaggtt atttcaaagt ggtaaaagat
    13321 ttattgaaac caaaaggaac agctttgatc catggaatca gccgacaaca aggcggtgcg
    13381 acaaatgctt ggatcaataa atatattttc cctggcggtt atgtccctgg tgttactgaa
    13441 ttagttgata atattacaaa aaacgatttg caactgattg atttggaaag cttacgtcgt
    13501 gactaccaat tgacattaga acattggaca aagaattttc atcaagtcca agatcaagtc
    13561 acgaaagaaa tgggtgattc cttctatcga atgtgggatc tatatttaca agcttgtgca
    13621 gcttcatttg aatcaagtaa tatcgatgtg attcaatatt tgcttgttca acctggaaac
    13681 aatgacatcc caatgcatcg tgcataattg aggcaaaagt actgtttaaa gactatttaa
    13741 aaagaagcta agttttatga tgctccagaa gtgaactttt ttgtctaact tctaggattg
    13801 tctctaaagc ttagtttctt tttctttata ttttcataat aaaagtttgg ttctttaatt
    13861 tttaaacatg acagaaaatt ttttcttcgt tatacttgta atagaatact attttataac
    13921 aaaggggatc gaatcaatga aaaaaactgt tatttatact agtttatttg cagctttgtt
    13981 tttagctgct tgtcaatcaa ataccaatca aggcacatct gggaatacgt cttccagtag
    14041 cgaacagcgc tcttcacagg tatcggactc ctcaacaaaa gcaagcacat cttcgtctag
    14101 ccagacaaaa agttctagtg aagcaagtac tcagtcagat atcaaagtgt ctgcagctcg
    14161 tgcgatcgaa ttgttccaag ataaataccc agatactgca attactagtt tggaattaga
    14221 tagtgattgg ggatcttact tctacaagat agaaggtgtc gatgatcaaa aagaatacga
    14281 agcaagaatt gatgctcaaa atgaaaaaat ggagacagag aatccagaaa ctctagatcg
    14341 agatgaacaa aatggtcaaa agaaaaaaga agatggtctg gatgttgata aattgatcac
    14401 gattgaggaa gcagcaaaaa tcgccgtcga caaagtagga agcggtacgg caacagattg
    14461 ggatttggat aaagatttag gtactactta ttgggaagtc aaagtaaaag acggcaatca
    14521 aagtacatca gtcaagatca atagccaaac aggcgaagta ctgtcaactg aacaagatga
    14581 ttaagaattt cttcggaaaa caagagttga tttttctttt agtcatgata cactagacaa
    14641 aataacttgt ttgaaggagc gatttacttg agtttagaaa cgacctttgc caatcatatc
    14701 tttgccaatc ctttgatgaa tgcatctggt gtccactgca tgactaccca ggaattagat
    14761 gaactggctc attcagaagc aggagcattt atcactaaga gctgtacgat caatgaacga
    14821 aaaggaaatc ccgaaccacg ctattttgat gttcctttag ggagtatcaa ttctatggga
    14881 cttccaaact taggtttttc ttattatcta gagtatgctc ttgcgtatga aaaagtgcag
    14941 gaaaaccaaa atcaaccgtt atttttttcc attgccggga tgagtgtcca ggaaaacttg
    15001 gaaatgcttg agaaaataga aaaaagtggg tttaatggga tcactgaatt aaatttatct
    15061 tgtcccaatg tgcctggaaa accacaacta gcttacgatt ttgaggccac ttatgaaaca
    15121 ctgaaagaag tattcagcat cttttctaag ccgctaggaa tcaaattgcc tccttatttt
    15181 gattttgctc attttgatca gatggctgat atcttgaatc agtttccatt gacctatgtc
    15241 aatgctatca acagtgtggg caatggtttg tatatcgata cagaacaaga agcagtagtc
    15301 atcaaaccaa aagaagggtt cggcggcatt ggcggagagt atatcaagcc gactgctttg
    15361 gcgaatgttc gcgcttttta tacacgtttg aaaccagaaa tccaaatcat tggtacaggt
    15421 gggattcgga caggacaaga tgcatttgaa catctgctat gtggcgcaag catgttacag
    15481 attggtaccg agcttcataa agaagggccg gaaatttttt ctagaatcat aaaagaatta
    15541 acgcagatta tgtcagaaaa aggatatacg tcaattgacg aatttaaagg caaattacgc
    15601 accatttcat agcaaagaga cgaaggtcat gattgatctt cgtctttttt tataaaaaac
    15661 actcgtctgt tcattcctgt gcaagcgtac tcattgtatg ttagacgaac ttgtgggaaa
    15721 ataacagagg caaaaaggag gataatatga atgatgcaac agaattagca aaagagctta
    15781 aagaaaaaga atttagtttt caagaactag ttacggatat acagaaaaaa ataaagaagc
    15841 aaaatcatga attgaatgcg tttgtcgcgc ttgaaccatt cgagagtaat tcccacgagc
    15901 tattgattcc ccagtcagaa aaagggccat tggctggtat cccatttcct ttaaaaatgc
    15961 tcggtcaaga aaaaaaggga tggctagcta cttcaggatc tcgtttattt gaaacacacc
    16021 gagcttctca tacatctaat tatgtgcggc aagcagaagc gataggtcta gttccttttg
    16081 gacaaaccaa tgcgccagag tttggtttca aaaatattac agacccagtc atctatggcc
    16141 cagcaagaaa cccttggaac cttgaatgta caccgggcgg atcaagtgga ggagctgctg
    16201 ctgcagtttc atcaggaatc gttcctttag ctggtgctag tgatggcggt ggttctatta
    16261 gaatacccgc ttcgttttgc ggattgattg ggctgaaacc ttcgcgaggg acgatgccag
    16321 ttggtccata tgcttggaga ggatggcaag gagctgctat tgatttcggc ttgacagttt
    16381 ctatgagaga cacggaggct ttattttatg ggatgagaag tatccattcc ggtgcgccat
    16441 atcaagtatc gccagttgag tggcaaacac atccaagaaa aaaacggttg aagatagctt
    16501 tttgtgttga ttcaccgatc cactcacaga ttagtgaaga ggcagtactt gctgtaaaaa
    16561 aagctgtaga tttccttgga aaagaagggc atgatttgac agagattcct tacccactag
    16621 atggtcgtaa actgatcacg tcttattatc agatgaatgg cgcagaaaca gcagcaatga
    16681 tccgctcgat tgaaacaggc atccagcgag ctgtcagaaa agaagaactt gaacccattt
    16741 cttggacttt gttccaatat ggtcagaagc tctctgccgc tacttacatc gattctctgc
    16801 atgtatggga tgaagcagca attgtcatgg aaaacctaca tcaagaatac gatctattcc
    16861 tttcaccaac gactgcattt accgccccta aaatcagcga agatttgcaa agtgatgcga
    16921 taagagcaca gatggaacac atcagtgatt gttctgaagt agaaggttta gacgttgttt
    16981 cttccatgtt tgaggagagc ttgaagatca caccttatac acagttggct aatctgactg
    17041 gacaaccagc aatcagcttg cctactcatt tgacaaaaga aaacatgcct ctaggaatcc
    17101 aatttatggc tgccagagga agagaagatt tgctatttca aatcggctat ctatttgaag
    17161 caaatcatca gttccattta ccaaatccat ggaaatagca gaaacaagga gaagaagaat
    17221 gaagaaaaaa agattgtttg ctctaataac tagtgccttt tttattttaa gcgcttgcgg
    17281 tacagctgcc gctaatcagg aagataacag ctggagtaat gtagaaaaaa agaaagaatt
    17341 agtagtagcg acttctggca cactgtttcc cgcatcttat tataatgatc aaaatgaatt
    17401 agttggttat gatgtagatt tggcaaaaga ggtggctaaa cggttaaatg tcaaaatctc
    17461 ctttaaagaa tacaacgtgg atggacaaat cacttctgtt tcaaaaagag aatcagattt
    17521 tgctgcgaac gattttggta tcacaaaaga aagagaaaaa aaattctccc tttcagagac
    17581 tttcaaatat tcatttgaca gtatgatcgt acgaaaaaaa gatgattcag gaatccactc
    17641 acttgaagat cttaaaggga aaaaagcagc tggcgaaccc aatactagct atatgcgttt
    17701 agcggaaaaa cacggagcta aattagtcac atatgacaat gccacgaatg atcagtattt
    17761 aagcgatgta gcaaatggtc gaacagatgt tattctaaat gattattatc tgcagaaaat
    17821 ggccgtatct gctttaccag atatccctgt aaaaatactg gagaacgttt atttcaatga
    17881 aaacgaaagt ggttttttat tgaaagaagg aaatgatgct ctgaaaaaga aaatcgatgg
    17941 cgtattaaaa gagatgaaag aagatgggac gacaaagaaa atatctgaaa aatattttca
    18001 aactgatgtt tcagtcaaac cgaaagaaac gatggctgag tcggtgagtg agtgatgtac
    18061 tttcccctaa ttgatttttc cctcatgtgg acttcacttc ctttcgtact gcaaggcttg
    18121 gtatacacat tagggattgg cttcgtgtcg tttcttttag gaaatttggt agggttgctt
    18181 ttgacagttt taggtctttt ggattggctg cctctgaatg tatttattcg tttttattta
    18241 tcgttcttta gaggcattcc cgcactagtc ctgctgtttc tgttatattt cgggttacct
    18301 tatcaattaa gtgccttaac ggcttcagtc atctgcttta cgattaccag cagtgcattt
    18361 attggtgaaa tctatcgagg atcactagca ggcgtttctt caggtcagtg ggacgctggt
    18421 ttagcttctg gtttttcttt ctttggggta ttgaaatata tcgttttgcc gcaagctttt
    18481 cgaatctcag taccggcact cagtaacgta gctatggatc tagtgaaagg tacttcgtta
    18541 gcagcaacaa tcacagtgcc agaaatattt caaaaagcaa agatcatagg cggtcggaca
    18601 ttcgattatt tttctctcta tattttagtt gcactgattt attgggtcat ttgtatcggg
    18661 atcgaaaaag tgcaacagta tttagaaaaa cgttctatcc atcagtcaat ctgaaaaata
    18721 gaaatggtgc tagcgtggct ggcaccattt ctattttttg tttcttgtat attctgccta
    18781 gtattttaaa accatttcat gtaaaatgta aatcgaaaat atgttcggag gtagaaaaca
    18841 tgaagacccc gctaaccatc gaaatagaat cctcgttata ccaatattgc atcgagcaag
    18901 gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca aggaattgta gatacgctta
    18961 gctgcttgac aaaatttgat ggaagccgag aatggcgatg ctatgagctg aaagtgacaa
    19021 aagcagattt tcgctctaca gccaaactgt catttgttgg acattataat tattttgttt
    19081 taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc tacaggaata ggtgttttag
    19141 tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc tggtacattt ctgattgcga
    19201 aaaaagcagc aaagaaagag cttgctgtac cggaagaagc tttgacaaac cgcttcatgg
    19261 cttctttatt cagggaagta agaaaagcaa agcaaatgga gcgaggcgta aaatattttc
    19321 caaaagatca attgtacaaa gaattgaaaa gacgttcaga aaatcaagat ccattttcta
    19381 acgaacgata ttatgaacgg tttttagagg aaatagctag tcaggcaatc aatgagttgc
    19441 aagaagaagt tatcgcgctt cagcatgatt atgaatattt gaggcaacaa cagcagatcc
    19501 gtcggttgcc tacagagcca ttggagtgaa aaatatgtac tatccttatt taagaggaaa
    19561 acaatttgac ctattagcat taaaagaagc actgtcgcga ggattgctct ccaataaaat
    19621 ccaaccagtg atcgaaccag tacgtgattc cgctacgctt aaaaatgtga tcgaattatt
    19681 tcaaaaaaag caccatccaa tcgctgtgat ccaaaatccc caagtaggac aattcaaatt
    19741 attcgatcag catgtgcaca gttggacagt caaagaaaat agctctgtcg tccctgcaca
    19801 gatcttaacg ccagaaaatc tcagtgaagt actggattca cctccagcgt ttcttgtttt
    19861 cgatggacag cactacccaa aagatacaga ggtgtggaaa cagttggcag gagttgattc
    19921 gaaatttttg ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa acaaaattgt
    19981 gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt tatgcagata aaacagatga
    20041 cttttttagt gatgactatc tttttttcca cgcagacgga tatattggat tttctgattt
    20101 cacgatcgaa ggtagccgtt atttcgataa agggttccct agccgagcgc ttgctatcca
    20161 tctaacgtat attgatgctt acggcaatat acgcgtgaaa cattttgtct ctgacagtaa
    20221 cgactctgct aaagaccaag cattgaagtt tcttgaagca ggagataaaa tgaaaaaatg
    20281 gatcatgcgt caccatcatc aactcttgat tacttcagga atgattgaat tattggctct
    20341 ttaccagcaa caaaaattcc ctggactggg agtgttgaaa aagtggtcac ttatgcatca
    20401 tttggaactt attagtcacc tgctagatca tccaaaagat tggcttaaat attattcaaa
    20461 agttcaggaa ctatatgaag tgatccaatg attagataat gtaaatgaaa aaggaataga
    20521 aaagaggaaa ctggatgtat attcggcaaa caaggaaaga agaagtagaa gaagtggtag
    20581 aaatcattaa tgccgcagcg gatttcattg cttcgcaagg ctctcctcaa tggcaaaacg
    20641 ggcagaaacc aacaaaagaa acgattttga acgatattga aagaaaagaa agctatgtat
    20701 taatagtgga agatcaaatt gctggaacag ctgcattagt ctcaggcgtg gatcccgttt
    20761 acacaaaaat agatggtagt tggaaagaat caactggtcc ttatttatcg attcatcgtg
    20821 tcgctttagc agaaagtact cgaggaaaaa aactagcaaa accgttctta gaacatttgt
    20881 tgacgattgc agccagcaaa aatatccaag atatccgtat cgatacttat ccagctaatg
    20941 aaccgatgga aaagaccatc tattctgctg gattcactta tcaagggatg attgaatttc
    21001 cttttgaaca cggcgaaaga aaagcatatc aaataatcat caattgacta taaaatcatc
    21061 tgagcaaaga taaagacatt aacactactg aaaaaaacaa tcatatgata gccaagaggt
    21121 tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg cttctcaaat
    21181 ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt ctgaaactcc cttcatcgag
    21241 aggtcgtgac atagcttttg tcacgatctc tcggcttatc ctacgagtat tattcgatac
    21301 atttatatca ataatccttt ttttatttta tctgcagctt cttggaacgc tccatcgttt
    21361 gacacgacta aaagtgaggg gatagctttc aaacgtttca actgtgctaa atcttctaca
    21421 taaaccgcca gtctggctga aatctgttca gatgaatctc cacgtttttt caggcgcgat
    21481 tgcaatgttt ctttagagat cgtaataaag ataggacgga ctaccgtccc gaaatactca
    21541 tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac tgtcagtttc
    21601 tttttaattt ctgctttact ggaaccatat ttatgttgag catattggtc ccactctgca
    21661 aactcatttc ccttgatcat ctgattgaat gtattgtcag aaacaaaata ataatcctct
    21721 tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag taatgatctt ttgttccggc
    21781 tgaaaaatag cttctgcgag tgaagttttt ccagaaccag aaggaccgat aaatacatag
    21841 caataattca ttgtcatttc tcctttgatg attgaacaag tgaaaaagct ttggaaaata
    21901 tttccaaagc ttttaaaagt aactttgctt ttttgtattt tataccagca agccagtcac
    21961 aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc cgtgttttgc
    22021 gacaagattt cctttttctt catttaatcc cttcatcgca ccagaaatga tcccgataga
    22081 agagaagttg gcaaatgaaa cgaggaagac agatataatt gctgttgtac gctcagtaaa
    22141 atggaaatta ccatttgcta attctgtcat agcaacaaat tcatttgtaa ccagtttagt
    22201 cgccatgata ctaccggcat ccactgcttc tttccatggg attccgctga tgaaggcaag
    22261 aggggcaaag atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc cgtggaagat
    22321 agcattgatc atggcaatca aagcaacaaa accgataagc atagcagcaa ccgtgatcgc
    22381 cacatggaaa ccatcaagaa tgtattctcc caacatttgg aaaaatgtct gttctttgtt
    22441 ttcttcaatg atcagctcat cttctttttc ttctaataca tagggattta caattgaagc
    22501 cagaatataa ccgccaaata agttcaaaac aagggcagtg atcacatatt tagaatcgat
    22561 cagtgccata taagatccga cgatcgacat agaaactgta gacatagccg atacactcat
    22621 ggtgaacaaa cgtttctcag ataggaaggg taattccttt tttatggaaa taaaaacttc
    22681 cgattgtcct aaaatagcag aagctactcc attataagat tccaatttgc ccatcccgtt
    22741 gattttactt aatcctagac caacagcttt cataaataat ggaagaatct tgatatagcg
    22801 tagaataccg ataatagcag aaataaatac aattggcatc aatacggaaa gaaagaaagg
    22861 tgcacttcct ttatttgcta caccggcaaa aacaaagtca acaccttgcc cagcaaaagc
    22921 aagaagatga tcaaacattt ttgccaatac agaaacaacc gctgtaccaa aagttgtacg
    22981 taataatacg aaaccaagaa caagctgtaa aacgaacatc acaattagtg gtttcaattt
    23041 gattgctttt ttgttactgc tgatcaaaaa agcaagccct aatgtgacaa ataaaccaat
    23101 aatacctaat aaataactca aataatcact cctattccaa attccgtttt attttacctg
    23161 ttttgcaaat aaaatactaa tctttagtga aattttcatt acttttttag agtgaaacgc
    23221 attcattttc cattagaaaa taaagcgaac aaaataaaag gtttaaaaag tgtaacgtct
    23281 tagtaaagtc tataaaaaaa cgaaactatc gattaagcta atagtttcgt tttcaacatt
    23341 tataagtaaa ctttttctat tggtaatata gattttcgga agggtagaca ggtgcgcagg
    23401 taatatcgat tcctagtttt ttcaatgttt gttcatcatt tttattgacc atcacagtag
    23461 agtgggcttg tgctccctca agttcagcta gtttatcata agcaacttga gcgattggat
    23521 tcgttactgc actgattgat aaagcgatca agacttcatt gatgcttagt gcatccaaag
    23581 aggaatgaag tgtctttgat ttcaatcctt gtattgtttc aagaactaat ggtgacaata
    23641 aaggaatgtc atctgcgata tgggctaaat gttttactgc atttaagata actgccgctg
    23701 acgcatccat caatgaactt gtacgcccgg taatgatgac ttcatctggt aattcgattg
    23761 caatcacagc agaaggatct tttgtaggac tttgcgcttg aatcttatct gcatagtcat
    23821 gggcagggac taccacgttt cggtcttcag gacgtaaacc gacttcttcc ataatcagtt
    23881 tgattcgatt aactgcttct tcgtcgatca atcctttttt gaagtcgcat tctgtttgga
    23941 aataacgacg gatgatttct tgtttagagg cttcttggac aacggtatca tccacgatcc
    24001 caaatcctac acgattcaca cccatgtcag taggagattg ataaacagat tctttaccag
    24061 taattttttc aataatacgt ttgattacag gaaatgtctc aacatcccga ttatagttca
    24121 cagctacttt attgtaggca tcaaagtgga aggaatcgat catattgaca tcttttagat
    24181 cgactgtggc tgcttcataa gcaatgttca gcggatgttt caacggaaca ttccatactg
    24241 ggaaagtttc gaacttggag taaccagctg catgtccttt ttggctttca tgatacagtt
    24301 ggttcaagca agtagcgagc tttccacttc ctgggccagg agctgtcact acaacgattg
    24361 gtttagttgt ttcaatatac tcattttttc caaaaccatc ttctgagacg attttttcta
    24421 cgttgactgg atagtcttcg atttctgagt gtttatagac cttgatgttt cgtctttcta
    24481 gtttattgat aaagactttc gtggcaggct gtccactata gcgagtgata acgacactgt
    24541 tgattaataa gccataacca cgtaattcat cgattaaccg caagatatcc atatcataag
    24601 taatcccata atcaccacgg atcttattac gttcaatatc tccagcatag acgcaaatca
    24661 gtatttcagc ttgatctttc aatttttgca gcaatttgat tttcgcatct tcttcaaatc
    24721 ctggaagtac acgtttcgca tgcttgtcgt caactaactt cccaccaaat tctaaatata
    24781 atttgtcata gtggttcact cgttccaaaa tatacgctga ctgctcttca atatattttt
    24841 ctgaatcaaa accaattttt ttcactaggt tccctcctta agtactaaag cattttttca
    24901 tttttttgag gattcgtcaa ctggaagagc gaaattcctc aaaaaaacaa agaaaatggt
    24961 ttaaaccaac ttgtctagat agttattcat tggtatgata gtttttgata aggaaggtgc
    25021 tacatgaaaa aaagacagaa aaagaaaaac gcatataaac actatatccg ttcgattttt
    25081 accggttatg aaagaatgct tgaagatccc gaattggaac aattgacgtt tacttatcta
    25141 aacgaagaaa cgcagctaac tcgtgatgag catcagcgca ttcatttcac aacaagagat
    25201 ctgcctagta aataaaaaat aagataaagg tcatcggatt cgattctgtc ctatcagatg
    25261 gaaagactag ttgatcgaaa aaaaggctgt accaaatttt ggtgcagcct gttttatgga
    25321 gcattgcccg ctctttttta taacaatttt ttacagaaaa tggtactatt gaagtattaa
    25381 taagtgaaaa acagatgagt aagaggaggc aagtagtatg aaagagccag atacaaatgc
    25441 aaattatgag ccagacgaac tagaggagac gaacgcggat caggatacag gacaagaatc
    25501 gtcttctttt tacgatatca aagaaaaagc aagcatgaat ctttcccaat tattagaaga
    25561 cttacaggct tttgaaaaag ctgtccgcga agaagatatc cctgagatct accgtttata
    25621 caatggccga ttgaatgaag aattaaaaag aagctcaaat gaaaatcacg agatcgatca
    25681 attgctgatg cgtaagatcc atgatcgttt tttacaggaa tttcctttta tggaacaagt
    25741 agaaaacatc tcgccaacga tgagctatta caaaatcggc acttattacc atgatcggcc
    25801 cacggtcggt attgatgcaa gtctgccaga aatttttgtt ttgcctaaga tcgatgaaga
    25861 gtgggagaaa tattcccaac cggagtcaac ggactatgac aaaaaaatca atgagctaga
    25921 cgcaaaggtg atcactgctc agacagaaat tgaacgttta gatgaacaaa taaaagaaat
    25981 caatcgacaa atgactgatt tgaacgacaa taaaggattc ttgaatcgta aaaaaatcga
    26041 ggaagaaatt caagaattag agaagaagaa gcaagtacta tctaatgaaa aattaggctg
    26101 gcttccttat attgagacgc ctgaaacaat ccagcagcaa aaagaagcat tgaagcaaga
    26161 agcaagagca gaccagcttc gtgcagccat cgtagaaaaa gaacaaaggc aaatcagacg
    26221 ctatttcggt agcaaagagg gattcggtca agcaatccat gaattcttga tgaattatct
    26281 tggcaatgag aatcctaata atcaatctaa tgaaggaggt tccgagtatg aatgaacacg
    26341 atgaagcaga atggacaaaa aatgaagaac aaacagaaga tcatgaagaa acgaacactt
    26401 cattggaaga acaaacaagt aatcctctgg atgacacaga agaaatccct atgctagagg
    26461 aaacaaaaat acatgaaaaa gaaaaagtaa ctccgacgaa tacgaatact gcagaaaaca
    26521 cccatgctta cgaaataact gaaaatcccg ggtatttaga aggaaaagag caagatcatc
    26581 aagatatcgt cagtttgatt gaaacagcgg aagcagaatt agcggattta cgtatgagaa
    26641 aagatttgat cgaatcagat tttccacaat tattagaatc atatgatcgt tttatattga
    26701 atgaagaacc aatctccacg atcggaaaga caagcctgat cgaatacttc acgtatgaat
    26761 tactgaaacc gttgaatgat attggtctgg aacggtcaaa ttcagcttat gacacatgga
    26821 aaacaagaca tttttcgatc aattatacgt tgaatggaca aaacgaatta gtcttttcct
    26881 ttgtattacc gaccatcgat caacgatacc aagtggaatc gatgagtctc ctaaacgtat
    26941 caccagagac aatggaaatc aatgtccaag atgaccgggt gctttctttg atccgacact
    27001 ggtcagtgga tcgagtattc tctgcagggc agatcacgat attcaatcac aaattgaatc
    27061 aattattagc ccatgcaaga acacttggtt ttaccgttaa tcagacatta ctagacaata
    27121 cgaagccact tcgtttgaat cttcagagcg aatttgaatt gacgaatgaa gtattggatg
    27181 atatttttat cgtggcaatg aaaaatccat cctacgatat ggaaaagatc aacgaaacta
    27241 cttatcaagt acgattagat aaaggacagt ctttaacgat aacaacaaac gaaaaagatc
    27301 aaacagtctt gtcgatctca tctggagact acccacgatc tgtcattgat ttctttatca
    27361 attacgaatt cttggtccca ttaatggtga gaaagtcctg attggataag ttggattacc
    27421 agatgtcatt cagataattc tgttccaaag gacttgcggc agttatccag aataattgtc
    27481 acaagtcttt tctgtatgta ctaaatggac gtgaataact tgctgatgaa ggagggattt
    27541 cattaggaat gtccacaaat aaaatagcac agcttagctt attgtctgct gcttgtgtag
    27601 caggtaggat cgcttttcaa tttattccga atttccagcc aatgactgct atttttttat
    27661 ttatagtact ttacttaagc ttaaaagatg cgttaattgt catgagtctt tctattgcga
    27721 tcagcagttt ttattttggt gttggaccat gggtcatcgg ccaatggatc agctatactg
    27781 ttgttctgag tttgttttca atcgtctgct tgagaaaaac agtgcaaagg aatctatggt
    27841 taaagggagt ctgttttttt ctggcgggca tggtttacgg aataggtatg accgtttttg
    27901 atacgctgtt atatcgtttg cctcaacctt ggatctatta ccttcaaggg gtgtcgtttg
    27961 atttgatgca cagtataggc aatgttgtgt tctttcttgt ttttctgcca gtcgtcaaac
    28021 gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa tttatagttt gcttgcaatg
    28081 agtgtcagta tactcatttt gacaagttgc agtacaacct atacacaatc ggcttccaac
    28141 caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca cgttacaaga aaatggtcaa
    28201 aacttttcgg aaaaagaagt ggtcttcaaa aaaggcgatg atttactgag tattttgaaa
    28261 cgaaattttg aagtcaaaga agataacggc ttcatcactt cgcttgaagg gcactcacag
    28321 gatgagaaaa atcaactcta ttggatgttt actgtggacg ggaaaagtgc tactactggt
    28381 gccaaagaga tcaaattgaa tgatggacaa tcggtcattt tcaatttgtc taaactataa
    28441 gtttcataaa aaactgttaa agggcgaata ttgccataat tcagtgagtt cagttgatga
    28501 aagaaggaaa ataacatgga aaattttaaa aaaagcggaa tgttttccgt aattgctgct
    28561 ctaggggcta atattctagt tgctatcagc aaatttgtcg gctatgcaat cagtggcagt
    28621 gccgccatgc taaacgaaag catccacagt atcgttgatt gcagcaatca gattttgctg
    28681 ttattcggtg ataaacaagc caataaagga cagagcgagt tgcatcaatt tggtgaagga
    28741 cgagcaaaat actttttcag tacaatcgtc gcaatgatgc ttttcttcgg cggtggggca
    28801 cttggggtga tggaagcttt tgaaaagttg acgcatccat cccatgaagt gggtaatccg
    28861 atcattgtta tagcaatact gttatttggt ttggtaatcg aaggaagttc gttacgcgtg
    28921 gcaatgaaag aaatcaaaga attgaatact gaaaaacttt ccacgatgcg ctttttgcgc
    28981 gaaagtcgac acagtgaaat actgattatt tttacggaag acttgtgtgc tgtaatcggt
    29041 ttggtcttgg ctttagcagg aacaatattg acttatgcaa ccgggattgc tgcatttgat
    29101 gcgatcagcg gattgctgat cggtttcttg ctgatgggag ccgccatttt cctagcaaaa
    29161 gaattttata gtctgatcat cggtgaaagc gtgacagcaa gcgatttatc aaaaatcaag
    29221 ttagcttttg aacgtcctga gatcagtcgg ctgatcgata taaaaacagt tcatttaagt
    29281 ccaacggaga ttttagttgc agcaaaagca gatatcgttg gtaacaaaga gaatgaagct
    29341 tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt ctcttcctga caagaagatg
    29401 tatatataca ttgaaacaga taaatatgac ccaaattatt ctcgtaaata aaacgtttat
    29461 tcatgaattg aagcaaaaat gagaaaaata taatcttgtt tttaaataaa acggctacaa
    29521 tttagcgaat ttatgttaca atgagtccgc tgtttatttg ttgtaattaa aggagatatt
    29581 ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt tgcatgcagt gatgccactt
    29641 tgtataagct atataccagt aggattagct tgcggagtat tgttgcaaaa agttggattt
    29701 gatcctctct tatcaggact gttatcgatt ctggtctttt ccggtggcgc gcagttttta
    29761 gttgcatcta tgcttacgac gcaagcatca tttgcaacga cattattgat ggttttcttt
    29821 ttagaattac gctatacatt attaggagca agtcttgccg gttttatgaa aaaagaaaag
    29881 cggacgtttt tggctgtctt ttctcaatca ctgaatgatg aaaactatgc ggtgaactac
    29941 ttaaagtttt caactgaccc ttcctggaat aaaagaaagg ctttatatgt caattggttc
    30001 tcaatgactg catgggccgg tagcaatatg atcggtaatt ttttcggctc ggtgattcga
    30061 gtagatgctg accttgtcca ttttgctttg acggccatgt tcattttcat gtttatcatg
    30121 cagatgaaaa gcgcactact gatctttaca ggtttattct caggcgtatt aggggtcgta
    30181 ttcatggtat tattccaaaa tactttcgga ttgattgcag caacagtcat tgcttcattt
    30241 gccggattta tgctggagaa ggcattcaaa aaagaaaaag ctaagaaact aagagaccgc
    30301 ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac aggaggccaa tcaccatgac
    30361 taaactatat ttgctacttt tagttgcttg tctgttcatc gtggcatata tcccgcgctt
    30421 gttcccgatg ctttatttta ctcatcgaaa agtgccatcc tggttcagtg actggatgaa
    30481 atatgtaccg gtggcattat ttgcggcgtt agcttttaaa gatgttttca ttactcatga
    30541 acatctggat attgcttgga atatcaaaat agctgcaatg attctagtag caggcgtggc
    30601 ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga cttgcgtctg tctttttgct
    30661 gtctatgctt taaactgttt tattttatta aaaacagaga ttgcgacata agttgttcaa
    30721 actgatctat ccgaactgca tttgcaacag tcaattctgg atgcaagttt attcgtaggg
    30781 ataaaaaaga ttagtgttgg cttatgatgc aagctctttt ttattttagg aattagagag
    30841 ggatttgtcc ttctatgatt ttttgctata atagggaaga tgtttatttt atgaaagaat
    30901 agttgaggga atttaaatga ataaaaaatt gatgcaagga acattttggc taacctttgc
    30961 taatcttcta tgtaaagttt taggggtagt ctatttgatt ccctggctaa gtatgatggg
    31021 gaacaatcaa gatggtatgc ttgctacgac attgtataat gttggctatc ttccttatgg
    31081 attgttcctg atgcttggta cagtcggatt tccaaatgcg attgcaaaaa aagtagctgt
    31141 agccacaaaa gaaggagata acgctgcttg tcggctgatt tttagaagta cgatcaatat
    31201 catgtttgtc atcggtatcg cctctgctgc actgatgtat ttatttgctc ctttactagc
    31261 cggaatcagt ccgattgcta acgttaataa tgggatacta gctatcagaa gtttatgccc
    31321 atctttgatt gctatcccaa ttcttagtgc aatgagagga tatttccaag gaaaaaatga
    31381 tctgcggcct tatggaactt ctttgattat cgaacaggct gtgcgagtga tcgttatctt
    31441 ggctggtact tattacttgc gcgtattgac tgacggaacc atattagaag ctgtattgat
    31501 cagtacagtc gcttctttct tcggtgggtt agctgcgatt atccatatgt atatcgtcgg
    31561 acgcagaaaa gacttcttcg aacttaaaga ttttttgatt tccagccgtt atttcgaaag
    31621 agaaaaccga tctgcctctg tctctatcat tcgggaaaca ctaccgttta tttttgtcgg
    31681 ctcagtcatt actatcgttc aaatgatcga ccaagtaacg atgaaaccac tgcttcactt
    31741 tttccgtcca gagatcgcag atcagcaact ggaattctta ttcagccgtg cttctgtcaa
    31801 tccaaataaa ttgacattga tcctgatctc catggtagga acagtagcta taagcagctt
    31861 gccaatatta agcacgatga agaaaaaaga tcggatacaa atcgaaaaaa cagtaggaga
    31921 tagtttttcg attgcgttat tgattcttct tccttcattg actggaatgt ctttattagc
    31981 tggaccacta tacacattgt tttttggcta tgatcctgaa agtgtcggat atttccaaat
    32041 ggctttgcta gcttctttgt ttttctcttt attcacgatc ctatctacga tgcttcaatc
    32101 tttgaatcac catcgttttg cgatccgatt aactgtagaa gcaattatcc tgaaagtcat
    32161 cttccaagta atcggattag gattagttgg cggatacggg atgagcttat caaatggctt
    32221 agcattcggt attgtctttg ttcgcggcta tcactttatg tgcaaagaat accgaatagc
    32281 acctttagca aagatcagca attttttctt aaaaactttc cgctccaccc tgattatgct
    32341 tgcagtatgt tttgccgttt tctttttgct gaatcagcga ttgtcaatgg aatcaaaaag
    32401 ctatgcaatg atttattgtg tggctgtagg aagtattggc ggcttagtat ttttattttc
    32461 tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt aaacatcata gcagaagcaa
    32521 agaataggtt aactaatcgt tttttgctgt cacttgaaaa acggcaactc gtatgattac
    32581 gcttgtcgtt tttctatttt ccaattttag tttcatatct aatcttactt atgatatagt
    32641 tataaggtct ggaaaaattc tttgaaacga ggaaattaaa cacatgaaac ttgcagtagt
    32701 tacagatagt tcggcatatc tgccggaacg tatcaagaac catcccgact tattcgttat
    32761 tccaatccct ttgattatgg atgggaaaat ttataacgaa ggaatcgata tcgaagcgga
    32821 tgaatattat ggacttttaa aaaacagtaa agattttcct actacttcac aacctgtcgt
    32881 aggggaagtt ttgggtttat atgaggaatt gaaagagaaa gggtatgaca cgattttaag
    32941 tatccatctt tcttcaggga tttctggctt tgtcaacaca ttatttgcga tgaaagatga
    33001 catcagcgga gtaacggtca ttccttatga ctctaagatc actagtatgc cgatgggaca
    33061 catggtagaa gctgccttag acttaaatga tgaaggtcag tcccttgatg aaatacttgc
    33121 acatatcgat cgaatccgag ataataccta tgcttatctg attgtagatg atttgaacaa
    33181 tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg attggcagct tattaaaaat
    33241 caaaccgatc ctgacatttt ctgaagggaa aattgtttta ttcgaaaaaa tccgttcaag
    33301 taaaaaagca tttgctcgag ctgaagaaat tattggtaga agaaataacg aaatacaacc
    33361 gccagtcaaa ctttacgtga tacatgcaaa taatttagaa gtagcgattg cggagaaaga
    33421 gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt ggtcatttcg gaccagtcat
    33481 tggtacacat ttaggtgaaa aagccatcgg cattgccatt tcagcgcaat aattcctttt
    33541 attcaaaaaa cataccaaaa ata
//
LOCUS       DACDHL010000022        30146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDHL010000022 DACDHL010000000
VERSION     DACDHL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.022"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IV228_001092"
     CDS             complement(61..2424)
                     /locus_tag="IV228_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP8717025.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="IV228_001093"
     CDS             2872..3093
                     /locus_tag="IV228_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8717026.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IV228_001094"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IV228_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8717027.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IV228_001095"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IV228_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8717028.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="IV228_001096"
     CDS             complement(5894..6205)
                     /locus_tag="IV228_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8717029.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="IV228_001097"
     CDS             complement(6202..6495)
                     /locus_tag="IV228_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8717030.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IV228_001098"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IV228_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8717031.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IV228_001099"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IV228_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8717032.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="IV228_001100"
     CDS             complement(8333..12685)
                     /locus_tag="IV228_001100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8717033.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="IV228_001101"
     CDS             complement(12897..14606)
                     /locus_tag="IV228_001101"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8717034.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IV228_001102"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IV228_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8717035.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="IV228_001103"
     CDS             complement(16103..16903)
                     /locus_tag="IV228_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8717036.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="IV228_001104"
     CDS             complement(16900..17712)
                     /locus_tag="IV228_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8717037.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IV228_001105"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IV228_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8717038.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="IV228_001106"
     CDS             complement(18467..19189)
                     /locus_tag="IV228_001106"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8717039.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="IV228_001107"
     CDS             complement(19325..20206)
                     /locus_tag="IV228_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8717040.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IV228_001108"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IV228_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8717041.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="IV228_001109"
     CDS             21446..21925
                     /locus_tag="IV228_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8717042.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="IV228_001110"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="IV228_001110"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8717043.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="IV228_001111"
     CDS             23226..24011
                     /locus_tag="IV228_001111"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8717044.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="IV228_001112"
     CDS             complement(24357..26048)
                     /locus_tag="IV228_001112"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8717045.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IV228_001113"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IV228_001113"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8717046.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IV228_001114"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IV228_001114"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8717047.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IV228_001115"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IV228_001115"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8717048.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8058 a         6464 c         5357 g        10267 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttg
//
LOCUS       DACDHL010000023        29621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDHL010000023 DACDHL010000000
VERSION     DACDHL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.023"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            151..1020
                     /locus_tag="IV228_001116"
     CDS             151..1020
                     /locus_tag="IV228_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8717049.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1185..2456
                     /locus_tag="IV228_001117"
     CDS             1185..2456
                     /locus_tag="IV228_001117"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8717050.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2453..3748
                     /locus_tag="IV228_001118"
     CDS             2453..3748
                     /locus_tag="IV228_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8717051.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3847..4110
                     /locus_tag="IV228_001119"
     CDS             3847..4110
                     /locus_tag="IV228_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8717052.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4555..5004
                     /locus_tag="IV228_001120"
     CDS             4555..5004
                     /locus_tag="IV228_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8717053.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            5061..6479
                     /locus_tag="IV228_001121"
     CDS             5061..6479
                     /locus_tag="IV228_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8717054.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6479..7339
                     /locus_tag="IV228_001122"
     CDS             6479..7339
                     /locus_tag="IV228_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8717055.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7513..8286
                     /locus_tag="IV228_001123"
     CDS             7513..8286
                     /locus_tag="IV228_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8717056.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8433..8798
                     /locus_tag="IV228_001124"
     CDS             8433..8798
                     /locus_tag="IV228_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8717057.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8946..10154)
                     /locus_tag="IV228_001125"
     CDS             complement(8946..10154)
                     /locus_tag="IV228_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8717058.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10173..10451)
                     /locus_tag="IV228_001126"
     CDS             complement(10173..10451)
                     /locus_tag="IV228_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717059.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10644..11300
                     /locus_tag="IV228_001127"
     CDS             10644..11300
                     /locus_tag="IV228_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8717060.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11742..12539)
                     /locus_tag="IV228_001128"
     CDS             complement(11742..12539)
                     /locus_tag="IV228_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8717061.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12536..13720)
                     /locus_tag="IV228_001129"
     CDS             complement(12536..13720)
                     /locus_tag="IV228_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8717062.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13745..14992)
                     /locus_tag="IV228_001130"
     CDS             complement(13745..14992)
                     /locus_tag="IV228_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8717063.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15207..16952
                     /gene="dnaX"
                     /locus_tag="IV228_001131"
     CDS             15207..16952
                     /gene="dnaX"
                     /locus_tag="IV228_001131"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8717064.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16972..17286
                     /locus_tag="IV228_001132"
     CDS             16972..17286
                     /locus_tag="IV228_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8717065.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17311..17907
                     /gene="recR"
                     /locus_tag="IV228_001133"
     CDS             17311..17907
                     /gene="recR"
                     /locus_tag="IV228_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8717066.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17971..18615
                     /locus_tag="IV228_001134"
     CDS             17971..18615
                     /locus_tag="IV228_001134"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8717067.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18630..18959
                     /locus_tag="IV228_001135"
     CDS             18630..18959
                     /locus_tag="IV228_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717068.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18973..19911
                     /gene="holB"
                     /locus_tag="IV228_001136"
     CDS             18973..19911
                     /gene="holB"
                     /locus_tag="IV228_001136"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8717069.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19947..20771
                     /locus_tag="IV228_001137"
     CDS             19947..20771
                     /locus_tag="IV228_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8717070.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20764..21111
                     /locus_tag="IV228_001138"
     CDS             20764..21111
                     /locus_tag="IV228_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8717071.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21180..22052
                     /gene="rsmI"
                     /locus_tag="IV228_001139"
     CDS             21180..22052
                     /gene="rsmI"
                     /locus_tag="IV228_001139"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8717072.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22161..23282
                     /gene="dinB"
                     /locus_tag="IV228_001140"
     CDS             22161..23282
                     /gene="dinB"
                     /locus_tag="IV228_001140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8717073.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23336..23938)
                     /gene="nrdG"
                     /locus_tag="IV228_001141"
     CDS             complement(23336..23938)
                     /gene="nrdG"
                     /locus_tag="IV228_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8717074.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24253..26442)
                     /gene="nrdD"
                     /locus_tag="IV228_001142"
     CDS             complement(24253..26442)
                     /gene="nrdD"
                     /locus_tag="IV228_001142"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8717075.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26768..27535
                     /locus_tag="IV228_001143"
     CDS             26768..27535
                     /locus_tag="IV228_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717076.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27525..29567
                     /locus_tag="IV228_001144"
     CDS             27525..29567
                     /locus_tag="IV228_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717077.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         9498 a         5321 c         6194 g         8608 t
ORIGIN      
        1 ggatatttta ttttttgcaa aaaacttctt gaaaattttc attttcattg attttagtag
       61 aaaactaccg aaatgtttgg taaaataggg tcgttggtta agaattgtct taacaaattt
      121 agttttacaa aacttaggag gaattttatt atgccagtag tatcaggagc tgaattttta
      181 aaagctgctc gtaaaggcgg ttacgctgtc ggcggattca acacaaacaa cttggaatgg
      241 actcaagcaa tccttgaagc ggctgaagca aaacaagcac cagtacttat ccaaacatca
      301 atgggtgcag ctaaatacat gggtggttac aaaatctgta aagatttaat cactgattta
      361 gttgaagcaa tgaacatcac tgttccagtt gctatccact tagaccatgg tgattacgaa
      421 gctgctttgg aatgtatcga attaggctat acttcagtaa tgttcgatgg ttcacatcta
      481 ccatttgaag aaaacttgaa attagctaaa gacgttgttg aaaaagcaca tgcaaaaggt
      541 atctcagttg aatgtgaagt tggttcaatc ggcggagaag aagacggaat catcggtaac
      601 ggcgaattag ctgatattga agaatgcaaa caaatggttg ctacaggtat cgactactta
      661 gcttgcggta tcggtaacat ccatggtcaa tatccagaaa actggaaagg attagctttt
      721 gaccacttac aagcaattgc tgatgctgta ggtccagaca tgcctttagt attacatggc
      781 ggatcaggta ttcctcaaga tcaaatcgaa aaagctatct caatgggtat ctcaaaagta
      841 aatgttaaca ctgaattcca attatcattt gctgcagcta ctcgtaaata tatcgaagct
      901 ggtaaagata aagaaggtaa aggattcgac cctcgtaaat tattagctcc aggtaaagca
      961 gcaatcgttg aagatgctaa atctcatatc gactggttcg gttcagctaa caaagcgtaa
     1021 gcatccacat aacagatacg atatatagga atggagaacg acttttgaag tcgctctcca
     1081 ttctttttta cttaaaacgg cagaccatgc tcgtttcttt tttttatcta tgataaaata
     1141 caagcatatc catgaaaaaa gattaataaa caaggtggaa acagatgaaa aaaatcgtaa
     1201 tcaaaggaaa ccgaccttta gctggtgaag taacgatcag cggtgcaaaa aatagtgcag
     1261 tcgcattgat tccagctgca atcttagctg attccccagt gatcttggaa ggtgtgccgg
     1321 acatacaaga tgttcattca ttaattgaaa ttttagagat catgggcgca actgtccatt
     1381 tttccaacaa catactagaa atcgatcctc gagaagtcgt atcgattccg atgccgcacg
     1441 gaaaaatcaa tagtttgcgt gcttcttatt attttatggg aacactgctt ggtaaatttg
     1501 gcgaagcggt agtaggcctg ccaggtggct gttatttagg ccctagaccg attgacctac
     1561 acgtcaaagg atttgaagct ctaggtgctg aagtgacaaa tgaacatggt gccatgtatt
     1621 tacggacaga aaataaagga ttgcgtggca atcggatttt catggacgtg gtttcagtcg
     1681 gagcaacgat caatgtgatg ttagctgctg taaaagcaaa aggacagaca gtaattgaaa
     1741 acgctgcacg agaaccggaa atcatagatg tggcaacttt actgaataat atgggggcaa
     1801 aagttcgagg agcgggtaca gatgtgatcc gtatcgaagg agtcgaaaca ttacatggct
     1861 gtcggcattt catgattcct gaccgcatcg aagcaggaac ctatctagca ttagcagctg
     1921 cagtaggaaa tggtatcaaa gtgaaaaatg tgatctttga acatttggaa agctttatag
     1981 caaaattgca ggaaattggt gtccatatga aaatcagcga agatgaaatc gaagtttacc
     2041 cctcgaaaga attaaaaccc gcaaacatca tgacctatcc ttatccagga tttgccacag
     2101 atctgcagca gccacttact gcgttactat tgatgactac aggcacttca gaaatcatcg
     2161 atacgatcta tgcgaaacgt gtgaaccatg tgccggaact agcacgaatg ggcgctgata
     2221 ttaccgtgga aggaaatatg atcatcgtga atggtccaaa taaacttcac ggcacagaag
     2281 tcgtggcttc tgatcttcgg gcaggtgctt gtttagtgat tgctggacta ttggcagaag
     2341 ggacaacgac gatttacaat gtagattata tcttacgcgg ttatgatcat attatcgaga
     2401 aactaacagc cttaggggca tcaattgaaa tgatagagga agtgggagct gaatgagcga
     2461 ttatttgaca atggcagaac tagagaacaa aacgttaaaa gaagtttata gttatgccaa
     2521 agaatttaaa attccttatt acagtaagat gaataaaaag gaattatctc tagctgtgat
     2581 tcgtgcacaa gcagagaaac aaggatttta ttatatggaa ggaatattgg atatcgttgg
     2641 tcaagatggt tacggttttt tacggccaat caattatgga ccaagtgcag aagatatcta
     2701 tatttctgcc tcacaaatca gacgattcag tttgagaaat ggggacaaag tggcaggaaa
     2761 agcccgtcca cctaaagaat cggaacgtta ttatggtttg atgcacgtgg aaagcgttaa
     2821 tggaaaagat ccggaagaag caaaagaacg tccgcatttt ccagccttaa cacctcttta
     2881 tccgcaaaga cagcttcaac tggaaacaac accgcaaaga ctatctaccc gaatgatcga
     2941 tatcttttct ccaatcggtt ttggtcagcg tggattgatc gtcgcaccgc caaaagcagg
     3001 gaaaacttct gtattaaaag aaatcgcaaa tggtatcacc gaaaactatc cggaagtaga
     3061 attaatcgtt ctactgattg atgaacgacc ggaagaagta acggatctag aacgaagcgt
     3121 gaaaggcgac gtggtatctt ctacatttga tctgcagccg caaaaccata ctcgagtttc
     3181 tgaattagta ttggaacgcg caatgcgttt agtcgaagat aaacgagatg tcgttatctt
     3241 gatggatagt attactagac ttgctcgggc ctataactta gtcgttccac caagcggacg
     3301 tacattgagt ggcgggattg acccagccgc attgtataaa cctaagaaat ttttcggggc
     3361 agcaagaaac atcgaagaag gtggcagttt gacgatttta gcgaccgctt tagtcgatac
     3421 aggaagccgt atggatgacg tgatctatga agaattcaaa ggaactggaa atctagaatt
     3481 acaactttca cgtgaattat ctgaacgtcg gatcttccca gccatcgata tcaaaaaatc
     3541 aggtacccga aaagaagaat tattgatgcc aagtgaagaa ttagaagaaa tttggaaact
     3601 tcgaaaaaat atgactggag attcattaga atatacggaa caactgatcc gctttttgcg
     3661 taaaacaaaa aacaatcagc aatttttcaa agaatttcaa gatgtctcct ttggtaaaaa
     3721 aaatccgacc agaaatctga aaagataatt gcaaccttga ctgaaacatg ttaagatgtt
     3781 aatgttgtaa atcgacaaat caactctggt tcagcaaatg gagcagggca aaggagcgaa
     3841 aaaatcatga aagaaaacat ccatccagat taccacccag tagtattcat ggattcaaca
     3901 actggtttca aattcttgtc aggttcaaca aaaacttcac aagaaacagt tgaatgggaa
     3961 gacggtaata catacccagt catccgtgtc gaagtaactt ctgactcaca tccattctac
     4021 actggacgtc aaaaattcac acaagcagac ggacgtgtgg accgtttcaa caaaaaatac
     4081 ggtctcaaag acgcaaacgc tgcggaataa taaaaacgct gataactcaa cgtttagcag
     4141 actatctgat agggatagtc tgttttttta tgttttttgc caatttaaaa cttcgtttga
     4201 tgctgaaaaa gtcgtaggat cataatgcac aaaacaatga aaaactagta tatagtttta
     4261 attatccact tgataaaaac accttatatt aacgaaatga agaggggtta tttcgaagta
     4321 aaaaaccatt tacctaaaat tagaagctaa gctttccttt caaggaaggc tttttgtttt
     4381 gttatagact tgcgattgtt tgtgagctag atcgttcatt ttaatgactt atacacgcgg
     4441 atgatgggtc tttttcctct gttcattcat aatattgaac ggtacagcta gcgatgctat
     4501 actgacctca gttccaagaa aacgttaaca ttttttgtgg gggagaggtt ttttatgagg
     4561 tttgatcaat tgatcgcaga agaactgatt gttttcgatc catcgataga ttcaaaagga
     4621 tcattatttg agcgaatagg ggatgtgttg aaaaaaacaa ataaggtaag aaatgttcaa
     4681 aaagtcgtta aggcactatt caaacgagag gaggagatct ccacaggaat agaagatgga
     4741 tttgggattc cccatgcaaa aagcaaatat gtgacaaaac cagcactgtg tttttttcat
     4801 acagccaaat taacggacta tagcgggata gatgaaatgc caattgaata tatttttgcc
     4861 atcgtagttc ctgaaaaatc agcagatatg catctagaga ttctcagcac actctccaga
     4921 cgattgatga atgcagattt tagaaaggga ataaaagaag ccactacgcc agcagatatc
     4981 atcagacaga ttagtcaaga atgaaaatag tagagtcttt gtccgagtat cgatagtgaa
     5041 gaaaaaggag gaaaataatc atgaaaatcg ttggtgtaac tgcttgcccg actggaattg
     5101 ctcatactta tatgtcggca gaaaaattag aagtaacagc aagaaatcta aaccatcaag
     5161 caaaattcga aacacaaggt gtaaaaacag aaaatcagtt atctgaacaa gaaatcaaag
     5221 aggcagatgc aatcattctt gcagtagata aagaaatcga attggaccga tttgccggga
     5281 aaaaagtaaa gcgtgtctct acaagccgag caatcaaaga accgcaagta gtaatagacg
     5341 aagcattgag agatattggc gtattcgttg tgtctaatga aaaagaacca gctgcaaatg
     5401 agaaaaagcc gacaatctat aatcatttca tgaatggtgt gaattacatg cttccatttg
     5461 tgatcgcagg cggaatcatc atcgcactga gttttgcctt tgggattaca gcggctgatc
     5521 ccaactcagc agactataat gtgttagcag ctgcattttc aaggatcgga ggagatacag
     5581 cgtttgccat gatggttcct gcgttaggcg cggggattgc tacatcgatt gctggaaaag
     5641 caggttttgc cccaggaatt gttgccggat tactggcctc tacaggtggt tcaggttttc
     5701 ttggcggaat gattggtggc gtgctagcag ggtatatctg tgattttctg gcaaataaaa
     5761 ttagcgtaaa aaaagaattt tctgctattt atcagctgat tgtcgtcccc tttttatcga
     5821 tcttaattat tgggcttttg atggtatttg tgattgaaca accgatcaca tgggtgttgg
     5881 atgctttgac aaattggctg aatagcttgg gaaatacatc cgggctattg ttcggattac
     5941 ttatcgggat catgatggca gctgatatgg gtgggccgat caataaatcg acttcgactt
     6001 tctcgatcgg tttgatgtca gctggagtaa atgcaccgat tgctgcttgt atggctgcag
     6061 gaatgacacc tcccctaggt attgctttag ccactttatt attcaaacat aaatttacca
     6121 aagaagaacg gacttcaggt caatcatgct ggattttagg cgcttcgtat attacagaag
     6181 gtgctatacc gtttgcggta tctgacccga tccgcgtgat acctagtttg atgacaggtt
     6241 cagcagtcgc tgcagggatt tccatggcag taggagttac ttcaatggca cctcatggag
     6301 ggatttgggt gatgctaatt ccaaatgtta tgaacaattt acctatgtat gtcttggcaa
     6361 ttgcatcagg tacagtcact acagccttta tgcttggact attgaaaaaa acagtgaaag
     6421 agaaaaaaca agacaagtgg acgaaaaatg aaaatctaac aaatgaggtg gaagcgtaat
     6481 gttatataca atgaaagagt tattgaaagt ggctaaagag aatcaatttg cagtaccagc
     6541 cttcaatatt tgcagctatg atatgatgaa agcaatcatc gaagaagcag agagattaga
     6601 agcaccagta atcttagaaa ttcatcctga tgaaatcgcc tatttaagtg atgagtttgt
     6661 tgcggcagta agagcctatg cttatacaag taaactgccg atcgttatcc acttggatca
     6721 tggcggtacg atagaagatg tcttacgggc gattcgaaac ggctattctt ccgtgatgat
     6781 cgatgcgtct ttgaaatcat tcgacgacaa tgtacagatt acgaaaaaag tggttgcgtt
     6841 agctcatgcg gtaaatgtct cagtggaggc tgagcttgga acgattggga ataatggttc
     6901 tgctgaagga ggtgctgaaa caatcattta tacagatcct gatcaagcga agatttttgt
     6961 gaaagaaaca ggaatcgata cacttgctgt agcaattgga acagcgcatg gcttgtatcc
     7021 gaaaaacaga actccaaaat taaatatcga cttactgaaa gaattgaacc aacaaatcga
     7081 tcttccattt gttttacatg gcggttcagg taatccggac gcagaagtca gcgaagcagt
     7141 aaaatacgga gttgccaaag tgaatttgag ctcagatctt aaaagcgtct ttttcaatgc
     7201 tttgagagaa atattgagtg aaaatccatc gatgtacgag ccgaatgcaa tttatccagc
     7261 agctaataaa aaggtacaag aagtggtcaa acataaaatg gaaattttga acacagtagg
     7321 caaggctagt ttatactaaa aataaaaatg tgtggtcaag agggtatgac aatagtcttg
     7381 tcataccctc ttaaccacac acgatgttca gaagtagttt tttcgaaaaa tttcttctta
     7441 tttctcgaag ccaatcactt ctgtcacaac ctcttttttc ctgttactat ataaagtaaa
     7501 tggggtgatt ttatgttgaa tgaagtggag aatagacagc aggagattgt gaattatcta
     7561 aaaaacaacc aatttgcaag agtcaccgat ttgatcgagt tagtaaatta tagcgaagcc
     7621 acgattaaac gtgaccttgt ttttctggaa aaaagtggat tgatccgtag agttcgcggt
     7681 ggcgccatgc ttattgacaa tcgaaaaatt gacgtgcctt acctgatgaa gatcaccaaa
     7741 ttagaagaag aaacagaaaa acgtaagatt gcagatacag cttcttcttt gattcgagat
     7801 gatatggtct tattcttgga ttccagtaca acctctttac acttagtcag aaacctagca
     7861 agatttgatg gtttgcagat cattacgaat ggcatcgtca cagctgctat gttgagtgaa
     7921 tatacaacgg caaaagtatc tgtattggga ggggcaattg ttccgaaacg agcaacaatc
     7981 aacggttcaa aagcctataa cgatgcattg tcatattatg cggatttagc gataatgagt
     8041 tgtcgcgggc tagatttcag ccaaggagta acagaaactc atgaaggaga ggcaatggtc
     8101 aaaaaagctt ttagaaaaca atcagaccgg cttgccgttt tagttacaca gcaaaaaatt
     8161 gcacataaat tcatgcatca agccattgct tttcatgaaa tcgattatct ggtgacaagt
     8221 aaagaactga caaaagaaca gtatgcgaaa gtaaaggaac accatatcga atgtctgtac
     8281 ggataaatta ttacgtgaat gttctaagtg gcttcctcat ttttagatag gaccttatct
     8341 ttgctcgctt ctctcagccg atggtataat aatgaaaagg ttatcttgtt agataatctt
     8401 acttttttat ccaggagaaa ggagtgcaga aaatgaaaca tgtgaaggcg ttggtagtga
     8461 aggcaatcat gatctgggcg attctgtggg tcgtgctgac aggtttatac ggtgttagct
     8521 ttatggattc aacgattgtt ggggtaatta ttgtagtcat gatctatgtg ctaggggatc
     8581 tgttgatctt acgtaaagta gggaacatcg cagcgacgat cgcagatgct ggttcagctg
     8641 cagttatctt gtggctctat ctatatttta tgaatgatac agtcgatatt tggatgaaag
     8701 tattgattcc tgctctattg atcggtgtgg cagaatggtt cttccacaag tggttgctaa
     8761 gtcaaggcat cgtaccagat gaacggaaaa tggaataaat caacagttaa actattctta
     8821 tcgataaatg aaagctttaa aaataaatag atgtttaatt aagaataatc ataaactatt
     8881 tttactcaaa aaaccgctca gattattttc agatctgaac ggtttttttt atctattcat
     8941 caagatcaaa tttcattttt caatgattca atcaattcgc cctctgcttc atgactatgt
     9001 tttcggccat taacaagata gagtgctgta catatcaatg gaatgactcc ggcaatcaca
     9061 tacaatcctc taaatgaagt aagagatttt agcatcccca tcaaataagg accaacgcct
     9121 aagcctaggt ctaaaccaat aaagtaggta gataaagcaa tactgattcg atgttcgcgg
     9181 acgattttta aacaaacagc ctgtccattt gacataaacg taccataacc taggccgact
     9241 aatgctccag agataagcaa tgtcacacta ttcgtcgtta cactcagtaa aaataatcct
     9301 gctgttaaga ataaatagct aggatacatc acgtattgtt ctcctttgac atcgaaaatc
     9361 cttccagtca atggacgtgt aaatgtgatg accaaagcat atacgacgaa gaaaaatgac
     9421 ccagcagcaa ctaagtggat tacttttgta taagaagata aaaatgataa aacacttgaa
     9481 taagaaagac ccatcaaaaa ggcaattcct gtaatgaaaa gtgctttctt ttcaataaaa
     9541 ctattgatat tccatgagcg aagagatatt ttttgttctt cagataatgg aatatttttt
     9601 acaggaaaga caaggcaggc aatcgtaaca aagaagacca gtacaattga aaaccagata
     9661 atgaaacgaa aatcagtcat attcagcaaa atcattccta aaaatggccc aacagcggca
     9721 gctaaactag tacttaatcc ataataattg atgccttccc cttttttaga tgcagggata
     9781 tatgcagtaa cgatagcatt tgtagctgta gaaactgtac cgtagccgaa tccattcaag
     9841 aatcgaacta aatacaatgc cccgatagtt ggcgtataaa gatatgctaa tgttgaaagg
     9901 agatagaaga tcgcgccgcc tcttaaggta aacttccgac caaatagttc taactgtttc
     9961 cccatgaata agcgtgccag caatgttccg atgatataga tcccagaagc aaaacctgct
    10021 tggctcaagc tggcgtgtaa ggtgtcttgc gcaatgactg ccatgatgac catcaacaga
    10081 taataaatca ggtagacaat aaaattgatc aatgtgattc caataaaata tttattaaat
    10141 aatttctttt ccatgtatgt tatacattcc ccttaagctg aatatgcttt taatttttgt
    10201 atttgaactt ctacatcgtg ataattggat ggatagcgat caacaagttg gcgaataaat
    10261 aaatctcttg tagacataaa cagatttaaa aattcttcgc attcgtttgt tggattctca
    10321 tccactgctt tgatcaattc gatcgagttt tgatacgcat catttaaaaa gactaaagaa
    10381 gctttcatat caaatcttgt ttgatatgct gtttctaatc gattaatgat tgtgttcatc
    10441 cagtaattca tgttttttcc ctctattctt aaaaaatttt acttctgcag tataacgaat
    10501 aaaaaaaagg aaaaaaatcg cataagtgat ggaaaaaggt acttaacaaa tgttaatatc
    10561 attagtaaaa tacctttgga ctttaaaaag ttttaaagta ctgtatgatc tttttagatg
    10621 aaagtttgga tgggggagca tcattggata gtagagtctt aaaggaatat ttggataaaa
    10681 atgattttcc gattatacgc aaaggatatc ataaatattt gacattcgaa gggatggaag
    10741 attcccatac gtatatcttg aaagagggaa cggtaaaagc aagtgttatt tcaaatgatg
    10801 ggagagaatt taaccttcgc tatatcaaag atcttgaaat cgtctctctt ttaaaagacg
    10861 aatattcaca attcatcgat tcgccgtata atattcgagt tgaatcagaa tatgcagagt
    10921 tttatcgaat caatcgtgtg cgcttttggc aaaatatcaa ccaagaccca aagctacaac
    10981 actatgtaaa agagtattac cgctatcgtc tgctttattc gatgaaaaaa atgcagcaaa
    11041 tgctattaaa cggaaagctg ggagctgttt gtacgcaact ttacgaatta tatgatttat
    11101 tcggtgtgcg gacagaagaa gggatgctga tcgatttttc gatcacgaat gaagaaatcg
    11161 ccaaattttg tggcatctca actgctagca gtgtaaacag aattttaaaa caactgaaaa
    11221 aagatggtat tatcaccgtt aaagaccatc atatcgttat tttgaatatg gacttgttag
    11281 aagacaatat tatcttttga tgaacaaaga ggttgtgaaa tcagcgtttt gacctgagta
    11341 ttagagaaac aaacggaaaa atagttcctc atattttgcc gttttttgag ctaataccga
    11401 gggtcagctg attgaacacc gtttaccaag aggttgtgag cctgccgttt aactctgagt
    11461 cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc
    11521 gaggagttgg taggagaaca ccgtttacca agaggttgtg aaaaagctgt tctgcatcaa
    11581 gtaataagaa gagccaagta aaaacagtct cttatgtttt taacttggct ggacttatca
    11641 ccgtagatgt aagcttttga acgccgttta tttgcaacaa aaggggctgt gacacgattt
    11701 ttgtcacagc cctttttttg cgaacattca tttagcaatt tttagaaact tctgccgcca
    11761 ccgccaaaag tcccgccacc gctagaatgg gtagtacttc cgccaccgcc gcttccagga
    11821 ggtgggcttt tggggattct tcttgtcgtg acaaaagaat ttgtcagacg atcggttttt
    11881 tcagttaatt tgatactgga tttttctcgg aatggatatt tgtagatgcc tgatttcaat
    11941 tgataacgcg aaacagtcac aaaataaaag acaagactaa ggataagagc caaaacaaca
    12001 gcaatgatga tttctactgt cgtcaaaacc ttatagcgtg taattttgcc agtttcttcg
    12061 tctacacgat aatgtcctcc tggaactccg tcttccacat atgttgctga tttagacagg
    12121 taagcttttg cagcattgta ataggcagca tttgtcatct gatcataaac atcatctaag
    12181 atcgaatcga ttctgctatc agtcaaataa tcgatcatat taccagaagt cgaaatgtag
    12241 atttcacgct gtcccatatc taatagcaat actgagccat tttgatcatt tccgactgcg
    12301 tctcttaagt aattatcagc aaagtctctt ggttcttctg aattagatgt agtcgttacg
    12361 atcaagactc gtcctttgat tttttcattc agcttttctg cctgttgttc caaagattgg
    12421 atttgatctg ttgtgaataa cccagcctca tcatttaccg tagctgtatc tgcatgagta
    12481 gccaccggcc agccaacact tactagcaaa agaaagaata aaaaaaattt tttcatcata
    12541 tcaagtaacc tccgatcaga aagaggatcg ctagcccagc gaaaatccca agagcggtca
    12601 gtcccaattt tttctggctg atcggcagga caccgctgac ctttcctgtt tgtccattca
    12661 ttgcgtagta gtagactttt ttacttgagt cattgcttcg ataagtaaca agccataccg
    12721 gcagtaatac gtaattattt acttcgtttg tgattgaagc agaagttcgc acaccggtca
    12781 atgttgtata tccatttgcg gtatctttta acagcttctc tgaatagtct tttaattcag
    12841 attgaatctg tgatttgatt gcctcatatt cgatatctct tttttctgct tggaacccgg
    12901 ctaaatactg gcttttaaaa tcaattgctt tatctaacgg gaaaggttgc acgccttcga
    12961 ccatcttttg ttgcgtattt tttgataagg cattttttac tagttcttta aaagaaagct
    13021 tgcctgctct tgaaacagca aattgcttcg tttctgtgta ttcgatgtca cctatccgcc
    13081 atatgcggat aactgtacca ttcgcctgca tggcaccatc tacttcagca tctgtagccc
    13141 aataagggaa atacacacct gtcaacttat cgatttgatc cttattgaaa aaagctttgg
    13201 gaacaaacca ttttttcttg gtccaagcga gaaatctgtt gattgcttct tctttctcga
    13261 cagcaaaagg aagtacttta tttggcaaga attctccgct taaacgtccc gatagtacga
    13321 cgggattatg gcagtagtaa caataagtcg cagcagttgt cgcatcagtc acgatctctg
    13381 caccacagtt gggacaaaga aaaagatcta tcgctgcggt attatctgca gattcgtccg
    13441 ctgtatctga aaaaccgcct gcctcgtcaa ccgctttgcg ctcggcttca ttcatatcgg
    13501 caagctgttc ttgggctgta aaagtaaaat cttcggtagg tgaaggttct tctttgcttt
    13561 cttcgccagc catacgcgct tctttttgct tttgctcata ttggctgact tcttcttctg
    13621 tatagatatt caggcagtat tcgcaatgaa atttttggtc tttgggatca aaagtcaacg
    13681 gaccgccgca attaggacat ttatgtgtaa gaacatccat cagctgcaca cctttctaaa
    13741 taaattaatt aacggggatt cctttaaacg gtttaccaca gtttggacag aatttaggga
    13801 tgccgttgga aagatccacg atctcattgc attcactgca tcgcatttgt agcttaggct
    13861 gaacgttttc tacaggtttg gcagctccgc agttggaaca gaattttccg gaattttctg
    13921 tcccgcaaac tggacatgtc caagtatccg catttccttg tgcagtttgt tgattttgtt
    13981 tttctgcttg ttgtttgatt tgttcttggt tattttgagc tgcttgagaa agataagaac
    14041 catttgcatt cattcccata cctacaccca taaagccggt cattgctcct gcttcatttt
    14101 ttccggctgc ttccatacca cgtgcaatgg aaccttgcac gtagccttca cgtacgctag
    14161 gatcacctag catcgcccct tcgttacgca tattgatcag tttcacagaa tcatcggtgt
    14221 aagaaatgct ggctacagcc acagaaacaa tctccatccc acgtaattca cgccaagaat
    14281 catccaatgc agtatccatg tatttgctta attccaaact cttagatggt acataagaaa
    14341 tccgttgacc atctgcagac atctgattga ttgctgattg tagcgctgtt aggaattcag
    14401 ctaaatactg ctcattgata tcattgattt ccacttgtgt tttgttttta gggattgcgt
    14461 ttgtataaaa taaaatcggg tcaacgacat gaatagaata agtgccatgt gcccgtaaga
    14521 ataattctgc gttatagaaa ttgtcaaagt agttcaacgg cgcgcttgta ccgaatttga
    14581 ttccctttat ttcttggaga ttgatataaa aaacttgttg tttctgcggt gtgacgccgc
    14641 caaatttgaa tcggtcaaaa gtttcagcaa ttgcttcttt cagtgttcca ttgaacatgg
    14701 aaggagctgc gtcattttta atggtgtaat aaccttcctc agcggtataa tcgatgattt
    14761 ttccaccatc gaccaaaagc atcatcatgt ttggatagac atggacgact gttccgtctg
    14821 ttaaaacatc ttctgttcct ttacgattgg agccacgttt gtcatctttg cggactttga
    14881 caccttttgt catgaccgtt gtatcactca tattgtctgg ttcgatgact tctagccatt
    14941 gatctgccag accgccgcca accatacttg ttgctgcttt gataagaccc ataaaaaatt
    15001 cctcctctga ttcagctagt gccaaacaat tattgtttat ttattatacc ataggaagat
    15061 gtgaaacttc tcggactaga gacggaattg aaatggaaaa ttgcgtattg tttaaatacc
    15121 tgaaaaagcc gagaaggata gtagtttttt tacctaatat cacgtataat aagagatagt
    15181 gaatttttta aaaaaggggg atccttatgg catatcaagc actttatcga gtctggcgtt
    15241 cgcagcgttt cgaagacatc gttggacaaa aagctgtgac acagacacta aaaaatgcta
    15301 ttgtttccca caaaacctca catgcctact tgtttacagg acccagagga accggaaaga
    15361 cgagtgcagc aaagattttc gcaaaagcaa tcaactgtcc gaacagtaaa gacggtgagc
    15421 catgcaacga atgcgaaatg tgtcgatcaa ttactgctgg tacgcaagaa gatgtgatcg
    15481 aaatcgatgc tgccagcaat aacggagtcg aagaaatccg atttatccgt gatcgggcaa
    15541 attatgcgcc tacaaaggca gcgtacaaag tgtacattat tgatgaggta catatgcttt
    15601 caacaggagc tttcaatgcc ttgttgaaaa cattggaaga gccaaaagaa agtgtcatat
    15661 tcatcttagc aacgaccgaa cctcacaaaa taccagctac gatcatttca cggactcagc
    15721 gatttgattt taaacggatc aatacggcag atattgtcga acatcttgaa tttatcttga
    15781 aagaatcgac tatcagctat gaaccagctg cattgaatgt gattgcccgt tctgcagaag
    15841 gcgggatgcg ggatgcattg agtattcttg atcaggcaat ctcgtttagt gaaggaacaa
    15901 ttactttaga tgatgccatg caagtgacag gtagcttaac tgacgaaatg atggacagtt
    15961 acttgagtgc ttgcgtgaaa aaagaagtac cagaagcatt ggaaacattg aaccaaattc
    16021 tcgcagccgg caaagaatca cgtcgctttc tagaggatat gttacaatac tgccgtgact
    16081 tgctgatgta tcagcaagca cctaacctag ttgaggaaca atcgagtacg atcagaagta
    16141 catttacaga attggcacaa actgtttcag cggatcagct gttttcaatg ataaagattt
    16201 tgagcgatac tcaaaatgaa attcgtttta ccaatagtgc cgctatttat ttggaagtcg
    16261 ctacagtcaa acttgcacag ctaccgtctg cttctgttcc ttcagcagat gtagcatttg
    16321 cagatgatca gatagtaaaa cagctacaac aagagattaa ggaactaaga aacgaaatca
    16381 aggaactacg cagtaataca ggaattgaga atacaaaagt acagccggta cagcaaacaa
    16441 atcataaaaa acagacaagt acctatcgtg taccgacgga acgagtatat aaagttctgc
    16501 gggaagcgac aagacagcat ctgaaccaag taaaagaagt atgggaagac ttgctgatga
    16561 gtctatctgt cactcagcga gcaatgctta aagcaagcga accagttgca gctggaccta
    16621 caggactagt gattgcgttt gattatgaga ttgtttgtca aagagcagca aatgacgaag
    16681 aattgcagct ttcggtacac aacaacttaa gccgaatgat caaagattat gcgcctgatc
    16741 ctgtttatat tacaagagaa agctggccag aacttcgaag aaattatttg acgcaggcac
    16801 aagaagaggg aacagaaaac tcttttgctt ctgaagactc agaaggagaa atcgagttgc
    16861 ttcctcaaga acaagaagta ccagaagaaa cacttgtagt aaaagcagag gaactgtttg
    16921 gctccgaagc tgttacagtg gtagatgatt aataaaaagg agtagataat tatgatgcgt
    16981 ggaatgggca atatgcaagg catgatgaaa caagtacaaa aaatgcaaaa agaaatggca
    17041 gaagcacaag agctgctaaa taaaacagag tttacaggta aagccaccaa tgaattggtc
    17101 aaagcggttt ttacaggcga tcgtcgcatg aaagatatcc aaatcaatga agaagtcgtt
    17161 gatcctgatg atatcgagat gcttcaggat cttgtcgtaa tggcagtcaa tgatgcgatc
    17221 gcacaaattg atgtggaaac tgagaaaaaa ttagggaaat acgcaaaagg tttgccagga
    17281 ttttagtttt aaactgaagg agtaatgaat atgcaatatc cagaaccaat tgcgaaactg
    17341 atcgacagct acatgaaact tccaggaatc ggtcagaaaa cagcgacaag actcgccttt
    17401 tatacgatcg atatgaaaga tgaagtagta aatgaatttg ctaaatcttt gttaagtgta
    17461 aaacgcgatt tgcatttttg tagtatctgc ggaaacatca cagaagaaga tccctgcgaa
    17521 atatgtagaa atccttcacg tgatcgaagc gttatcctag tggtcgaaga gccgaaagat
    17581 gtaatggcaa tggaaaaaat gcgagaatat cacggacttt atcatgttct ccatggtgtg
    17641 ttatcaccga tggaagggac aggtccagaa gacatcaata tttcttctct gttgcagcgg
    17701 ctgcatgatg cagatgtaaa agaagtgatt atcgcaacaa atgcaacaac agagggagaa
    17761 gcaacagcta tgtatctctc acgtctgatc aaacccgcag gtatcaaagt gactcgtcta
    17821 gcacatggtc tgtctgtagg ctctgatatc gagtatgcag atgaagttac tttattgaaa
    17881 gctgtcgaag gaagacgtga gttataaatt tctaattaag tgttttttca gatacaatat
    17941 aaaagcagca caagagatgg gggcaacaat gtgaacggac tatttattac aatcgagggg
    18001 cctgatggtg caggaaaaac cagcatcttg aatgaacttt accctctttt gaaagaagta
    18061 gcaaaaacag aaatcattca aacaagagaa ccaggaggca ttccgattgc tgaagagatc
    18121 cgagcagtga tccttgatcc aaaaaatgat cggatggatg aacgtacgga agcactgctt
    18181 tatgcagcag caagaagaca acatctagtc gaaaaggttc tgcctgctct ggcaaaaggg
    18241 aaaatcgttt tatgcgatag atttgtcgac agttctcttg cttatcaagg tgcaggccga
    18301 cgcatcggcg ttaccgagat tgctcggttg aatgagtttg ctacagaagg tactacgccg
    18361 gattttaccc tttatcttga cgtagattca gacaccggat taagaagaat caagaaaaac
    18421 agacaaaatc aaatcgaccg tttagattcg gaaggtttgg aattccacca gcgtgtacgt
    18481 catgcttact taaagctggc agaagagaat ccggaaagaa tccacaaagt tgatgcaaga
    18541 aaaagctttg aagaagtgct gcaaacaagt tatcatacaa ttattgaaca gtatccgcaa
    18601 ttttttgaaa actaggaagg tgaacgttta tgaaaattat tttagcgatt atccaagaca
    18661 aagacagcaa ccgtttatca aatgaattga ttgatgcaaa tatccgtgcc acaaaactat
    18721 catcgacagg cggcttcctt aaggctggaa acagtacgtt tatcgtgggt attgaagatg
    18781 accgtgtaga agaagcattg gaaatcatca aaaaaacgtg tgaatcaaga aagcaatttg
    18841 tatctacacc tgttacactg gatatttcga tggatggggg cgtcccttat ccagtagaag
    18901 tagaagtagg cggtgccaca gtcttcgtct tacctgttga aggattccat caattttagg
    18961 aggagctgag ctatgaatca agaaataaac ttggctcaaa tgcaaccgat cgtgtatcag
    19021 cagcttcaac ggagttttga gcatgggcgt atcgctcatg cttatctttt tgaaggggag
    19081 aaaggaacag gaaaacatga agtaggtata tggcttgcac agcacctgtt ctgtacccaa
    19141 atgaaagaca atctgccttg tggaaaatgc aacaattgtc agcggatcga aaagaaagaa
    19201 catccagatg ttttggttat tgaaccggaa ggacaaacca tcaaagtcga tcagatcaga
    19261 cgtttgcaga cggaattcag tcgcagcggt tacgaatcaa gaaaaaaagt ttttttgatc
    19321 aaagaagctg aaaaaatgaa tagcagtgcg gcaaacagtt tgttgaaatt cttggaagag
    19381 ccccctggag attttttagc ggtgttagaa acagatgcag taggaagaat tctccctact
    19441 attcaatctc gttgtcaagt tctgcatttt caagaacttt cgaaagaagc actgatccat
    19501 cgattgcaag aagagcagat tccattggaa aaagcaaaac ttttggcttt cttaacaaac
    19561 agtttaggaa aagcagttga aatctctcaa gatgaatggt ttaatgatgc taaggacttg
    19621 atttatcaat ggttcgttta cttgcaaaag caagatactc aagcctttat ctatgtccaa
    19681 aaaaaattgg taaaatcatt taaagaaaaa acacaacagt ttacaggatt atctgtcttg
    19741 atgttttatt atcaggaagc acagcgtcaa gcagtagcaa acggcgaata tggcaaagtg
    19801 aaacagataa accagataat tgaacggata ttgatcgcag agcagaaact acgcagcaat
    19861 gtcagttttc aggctgttgc ggaacagttc gttttgcata cgatttttta gcttgccgca
    19921 aaggatgatg atcaaggagg atggaaatgg tagaagtagt aggtgtccga tatagagatg
    19981 caggtcatat ctattatttt gcaccaggag actctgaata tgtttatagt gacaaggtat
    20041 tagtcgaatc tcagcaatcc ttacatattg ctactgttgt ccttcctaaa aaagagattg
    20101 atcccaatga ccttcctgaa gctatccgtc cgatcttgca taaagcgtca caagctgaat
    20161 tagaaaaaga caaaaaaaac aaaaaagatg cttatgatgc attgaaaatc gcaaaagaaa
    20221 aagtgcagtt gcgtgagttg gaaatgaaaa tggtcaaagc cgaatacaca tttgatcgaa
    20281 ataaattgat tttttctttc acagcggacg ggcgaattga ctttagagaa ttggtaaaag
    20341 atctggccgc tgtatttcgt actcgtattg agcttcgaca aattggtgtc cgcgatgaag
    20401 caaagataat aggcggaatt ggtccatgtg ggcgtccatt atgctgttct tcttttttag
    20461 gcgatttcgt gcctgtatca atcaaaatgg caaaagatca aagtttatct ttgaatccaa
    20521 caaaaatttc cggtttatgc ggacgcttga tgtgctgtct gcaatatgag agcgaagaat
    20581 acgagacagc gaaaaaagaa ctccctgact ttggaaaagt aattgctaca cctgatggta
    20641 aagggaaagt cgtagggttg aatctgctta gccgtatcgt tagtattcgg ctgactggac
    20701 gggaaaatcc tgtcgaatat gaatgggatg aattgaaaga atttatccaa gtaggtgaga
    20761 tgaatggata agagatcatt atacgacgga ttgaatcaat tagaatcaga gttgagaaat
    20821 acattaacag aacttgttga aatgaagcat gctttgcatg agatcgtaga aaaaaataca
    20881 actttggaga tggaaaatca acgtctgcgg gaacacctgc gggaattgaa ccaagttgca
    20941 caagctccag ctgaaaatgt gaagcaggaa ctttctaagt ctcgtatgaa tcttgaaaaa
    21001 atttatgaag aaggattcca tgtctgctac gatttatatg gatcaagaag ggacaatgac
    21061 gagccttgtg cgttttgtct agaagtgatc tatcgcgaaa gcggtcgatg accaataaca
    21121 agaggagact ggagcggaga aatactgctc cagtcttttt attaaggaga ttgtgacaga
    21181 tgtataatca aaaaagtttc aagggaaatc cggtatatgg ggatttatat cttgtaccga
    21241 cgccaattgg aaatttagaa gatatgagct acagaagtgt tcgtatgctg caagaatccg
    21301 atcttatcgc aagtgaggat acgcgtaata cacaaaagtt attgaatcat tttgaaatcc
    21361 atacacccca aaaaagttta catgaacata attataaaga acgtgtccct caattgattg
    21421 ctttgttaaa agaaggaaaa acgattgctc aagtcagtga cgcaggaatg ccttccatca
    21481 gcgatcctgg acatgagcta gttttagcct gcataaaaga agggatttct gtgatctcta
    21541 tcccaggacc tacagctggg atgacggctt tgattgcttc agggctagtg cctcaaccat
    21601 ttttatttta tggcttcctt ccacgtaaga aaaaagaaca gaaagaaaca ttgaaaaagc
    21661 taaaagaaca gacagttacg ctgatttttt atgagtcacc ttatcggatc actgctacat
    21721 tgacgaattt cttagaaatt tttggaaatc gccaagtcgt tttgtgtcgg gaattgacaa
    21781 aggtccatga agaatacctt agaggaagta cggaagagct actcgcttat atcgaagaac
    21841 atccagtaaa gggtgaatgt tgtctgcttg tagaagggaa tttgttcgaa atgccagtga
    21901 cggaagttga tgaagagttg ggaacgttaa aggaacaagt agaagcaaag atcgcttcag
    21961 gcgaaaaacc aaatgctgct atcaaagcag tagcgctgaa aaatgggctg aaaaaacaag
    22021 aagtctaccg tcagtaccat gaattagact gagatagtat aagttgtcta taatgaaaga
    22081 ctaatgagga aatcagctac taacttttca aatctcttga acgcgaacga atgttcttgt
    22141 ataatgaata agaggtgagg atgatggagc tgcaatttcc tttacataat gatacatcaa
    22201 gaaaaatcat tcatattgat atggatgctt ttttcgcttc ggttgaagaa cgagatcatc
    22261 ctgagtttcg cggacatcca ttagtaattg ccagacaccc cagtgacact ggtgggcgtg
    22321 gagtagtgac tacagcaaat tatgaagcaa gaaaattcgg aattcattca gcaatgagtg
    22381 cacaaaaagc gtatgagctt tgtccgcaag ccatctttaa gcctggcgac catcataaat
    22441 acagcgatat ttccaaacaa gtacgggaag tattctatcg ttatacagat attgtggaac
    22501 cagttagtat cgatgaagcg tatcttgatg tgaccgaaaa taaaatcaat tgcaagtcag
    22561 ccatcaaaat cgcccgaatg atccaagcag atatttggga gagtgtccag ctaacatgtt
    22621 ctgcgggagt aagttacaat aagtttttgg ccaaattagc atcagactat caaaaaccta
    22681 gaggaatcac ggtagttgcc cctcaagacg ctgtgccatt tttaaaagct ttgccaattg
    22741 aaaaatttca tggggtaggg aagaaaactg tacctagaat gcatgagcta gggatttata
    22801 caggaaaaga cctatatgaa tgcacagaaa tgatgttgat tcgaaatttt ggaaaaatgg
    22861 gctattctct ttatcgtaaa gtcagaggaa tccatgattc tccagtaaac gttacaagag
    22921 agcgaaaatc tgtgggaaaa gagcatactt atgggcagcc gttgcaaacg gaagaagcag
    22981 tgctgactca gttgcgccaa ctagctgaaa aagtagaaga agcactgaga agagtccaaa
    23041 aacatgggaa aacagtagtt ttgaaggtca ggtacacgga ctattctact gtgaccaaaa
    23101 gagtgacatt gccagaatat atctataaaa aagaagcact cttttatcaa gcaagcctta
    23161 tatgggagga aatcttggga gtagaaaaag gaatccgatt gttagggatt actttgacga
    23221 atctggaccc gatgacttat gaaaacatcg tccttccatt atgggaaaat caagaaatat
    23281 aaaaacagct ttgagagttg aaaccggaaa tccgttttct ctcccaaagc tgtttttata
    23341 tcaagtcttg ctttttgatt tgttcatagg tttctgttgc atccgtacat ttttcccaaa
    23401 tcacaacttg attggattct aaagattttt ggacgtcaat gatccgctgg ttgctacttc
    23461 cgcgaaattg tagttttagg tcacgtttgc ttaattcaaa gcgtccgtca acaaggatat
    23521 cgatttgcga aaggagttct aatttatctt ctgaatcttg tagcaattca tcaaaggtat
    23581 agcctgtcca tgaccagata tcttttgtcg taccgaactc ttttcggata cgtcgtacga
    23641 cttgcagaca aacatctgta ttcaaaaaag gttcgccacc aagtaaagtt aatccttgca
    23701 cgtaatcgtt gcgcaagtca gcgatgattt gttcttctaa ttcaggtgtg aatggtttgc
    23761 catatcgaaa attttgtgca gctttgttga agcagccttc gcaggcaaat aagcagccac
    23821 taacgtaaag actgttgcgg acaccttcac catcgacaaa attaaacggt ttgtagtcag
    23881 caatatagtt ttggctgtag tcttttgcta accattcttt tggttttgga tttctcataa
    23941 aaaaacttct ttcatttaag gttcaaagat atagctattg aagataaaat ctactcgacc
    24001 acataaataa gaacaaactg acctaaatcc attgttcaag aaagcttgtt cttattttga
    24061 tagagggcgt tcaaagatac aaagccatcg aaagagggtg gtgaaaaaag acttgtcata
    24121 gcctcttatt ctgaataaac ggtgttcaaa agcttgcatc tgcagtaata agcccaacta
    24181 aacgaaaaat atgaggaact atttttgttt agctattctt attacttgat gcaggacagc
    24241 tttttcacaa cctcatttca tgtgtttcac acgtgaggaa atttctttat ggcggccatg
    24301 taccatcgga cgagcttgcg gatttcctaa atagccacaa gtacgtttga ctacatcaca
    24361 tgtttttgga tcatgatttc cacattctgg acattcgaac cctcgttctg tcggtttgaa
    24421 atcaccttcg aaaccacatt gatagcaatg atcgattggc gtattcgtac caagataccc
    24481 gattttgtca tatgcaaaat cccatacagc ttctaaagct ttcggatttt gttggagcat
    24541 agggtattca cagtagtgga tgaatccacc tgaacaatat tttggataat ccttctcaaa
    24601 ttccaatttt tcaaatggcg ttgggttttt acgcacatca tagtggaaac tgtttgtata
    24661 atattcttta tctgtgatat tttcgatgat gccgaatttt tctgtatcca aacggcagaa
    24721 acggtcagtt agactttcac ttggagttga atagacactg aaatgataac catattcatc
    24781 cccccatgca tctgcattag cttttaattc tttgacgata tcaagtgtga aagcttttgc
    24841 ttctgggttt gtttcccatt cgccaccgaa gaaagctgaa gcaacttcat acaatccaat
    24901 gtagcctaac gatacagtag ctcgacggtt tttgaataat tcattgacag aatcggtacg
    24961 tgaaagacgt ttcccgaaag caccatacat atataaaatt ggcgcatttg ctggaatggc
    25021 ttctttacaa cgttctacac gatagagaag cgcatctttc atgatttcta agcggtcaga
    25081 cagtaactgc cagaatttat tctgatctcc ttttgattcg atcgcgattc ttggcaaatt
    25141 caatgtaaca acacctaaat tcattcgtcc gacatttact tcttgaccat tttcgtcttt
    25201 ccacccttgc aagaatgaac gacagcccat aggtactttg aaacttccag ttaattcaac
    25261 gattttatcg taattcagaa tatcaggata cattcttttc gttgcacatt ccaatgctag
    25321 ttgtttgatg tcgtaattag gatcttcagg gtttaagttc acaccttttt tcaaagtgaa
    25381 gatcagttta gggaagatag ctgtccgctt ttcactccct aatccattga ttcgaatctg
    25441 taaaattgct ttttgaattt ctctttcaaa ccagttttct cctagtccaa atcctaatga
    25501 agtgaaagga gtttgtccat ttgaagtaaa taaggtattg atttcatatt ccaaactttg
    25561 catagcatcg aagatatctt ttttggtttt tgcttttgca tattcttttt ggcgttctgg
    25621 cgaatcgatc cattcttccg cgtcttttag atgtttttta tagttcaatt ctgcaaaagg
    25681 agcaagcaat tcatctgttc ggtcggcaga acagccacca tattgacttg acgctacatt
    25741 ggcaataatc tgtgagattt gtgctgtggc ggtctggata gattttggcg attcgacttc
    25801 ggcattgccg attttgaacc cattgttcag catgcctttg aaatcaatca agcagcaatt
    25861 cgtcatcggt gtataaggat gataatccaa atcatggtaa tggatatcgc ctttttgatg
    25921 cgcatttgct atatgtggtg gcaacatttt cagtccgatt gattttccaa cgatcccggc
    25981 tgtcagatct cgctgtgtat tgaaaacatt actgtcttta tttgcgtttt cgttcacgac
    26041 agtttggtcc ttgttgatta gcttttcaat cgaaaagttg atatctgttg ctttactgcg
    26101 agcaaaatct cgtttggtac gataattgat gtactcttct gctaattcat attcgttttt
    26161 ttctagtaat atatgttcca cgatgttttg aatctcatag attttgacat tttcagcaaa
    26221 ccggcttttg atttcttgga caaccaagtc tgcaatcatt tgaatttttt cataagtaag
    26281 tggtgataat gagccgtgaa ttttcttttc tgctttgatc agtgcttcat tgatcttttg
    26341 ttcatcgaaa tcaacatggc ggccatcgcg tttgatgact ttcatctggg atgtctctgg
    26401 ctgctgagta acgacagttt tttcttcata atttactttc attatcttca actcctgcta
    26461 aattgttctt gtctatcata aaacaaaatc ggggaaggag caactaaata tcggctatat
    26521 atagtattca atgtgctgta taattggatt tctatactat atgtagtgta tattttattg
    26581 tgaaaaaagg tgaaaacttg tagctggtgg atttgttttt tgcatattta attttgctga
    26641 ataaaataat tgaataaaat tttttattca gtccaagtaa aagtgacaaa aacataagag
    26701 caaattattc gtagatatgc tactatgcaa ataaatagaa atgaaaaatg aaagaggagg
    26761 agaagaattg gaaaagatga tcgaagtgaa acatctaagt aaaagctacg gcagcaaact
    26821 ttctcccgtc aaagccttag ataatatctc attcacagta gaagaaggcg agtttgttgg
    26881 gattatgggc ccaagtggtg caggaaagac aacgctgatg aatatcttgt ccacgatcaa
    26941 tttgcctacg atgggcagtg tctcgatcca aggaaaagaa atcacaaaaa tgaaaaacag
    27001 tgagctcagt gactttcgtc gaaaaaaact aggattcatt tttcaagagt tcaatttgat
    27061 cgatacccta aatgcaaaag acaatatcct attgccccta gcagttgaac ggatgactaa
    27121 agaggaaatg gaaaagagag taagacatgt agctcaattg ttgaatatag aagaactgct
    27181 caaacggtat cccgatgaac tttctgtcgg acagagacaa cgaattgcag ctgctagagc
    27241 acttgttgtt cagccacaag tgatttttgc agatgaacca actggctctt tggactcgaa
    27301 gtcggctaca gaattgttga actacttgaa gaccatgaat caaaaagaaa aagcaacgat
    27361 actgttagtc acccatgatc catatacagc aagctactgc gatagaattt tatttattaa
    27421 agatggtgtg attttttctg aagttgtcag aagagggaca agaagagagt tctttgagaa
    27481 agtaatagac atgcaggcga cgattggcgg aggaggaaaa atgaatgctg tataaactag
    27541 ccgttaaaag tttttttcgg cagtcacgag gctatcttgt ttattttttc agtttgactt
    27601 tatccgtaat gatctattat tccttcagcg caatgaccta cgatcaatct ttagtcagaa
    27661 gagcaagtca agatacttcg atagatggtg gattgagttt tggtggattt atcattactg
    27721 ttgttttgct ttttttcgta ttcagtgcca accgattttt cttgaataga aggcaaaagg
    27781 aaataggcat ctatcagctt tttggtatga ataaactgca gatatcaggt atatatgtgt
    27841 tagaaatcat gataattgga ttgttcgcct gtatttctgg gatattactc ggtattattt
    27901 tttccaaact cttttcaatg attttggttc gaatgatgga tatggatctt actagtccat
    27961 tttttatctc cattccatca gtagtagata ctttatttgc ttttttcatc atccttttag
    28021 cagtatctgt atattcaata tggaaaatat ggcgctatcc aatgattcga agttttggag
    28081 aaaaagaaca agcagatagt tcaacaatgc gcttcaggac aaggcaccga ttactcgcag
    28141 tcatcgggtt actactttta acgagcggat attttggagc tagtcatttt agggaaatca
    28201 cttcatggat gatcagttat cattatcttg atacattagt tttctttttt ccttttttaa
    28261 tttttttcgt ttgtgtggtc ggtacttatt tgtttttctg ttatacattt cggttatttt
    28321 tgatcatatt gagtaaatgg aagatttatt actatggtat caattttttg ataatgggta
    28381 acacgcaagt tcatttgctc aaaagttggc gtacaaattc tctgattaca gtcattctcg
    28441 gaatttcttt aatgacaatt ggcggcacgg ctagtatctc tactatttta tcctaccagg
    28501 agaaagtttc ctccccgatg gactatcagt ttgatgttga gacagagaaa aaaatcaagc
    28561 cgattctagc agaggaaggg cagaagataa ctaaagaaat caccttgcat tataaagtca
    28621 ttgggagtat ctatagtcaa aatctcgaag aacctatgtt tcaaactgct aatctgatca
    28681 cggaaacaca atatcaagcc ttcagaaaag ttaatccaga tttgccgaac atttctttga
    28741 aatcacctgg tcatactgtt ttgcttgatc aaatgcaaac tctttttcgg aatatttcat
    28801 tatatggatc tagtattgga ctgccagata atcaagaatt gaagattcaa caaatgcttc
    28861 caagtgtatt aggtgacgaa acaatggtat atctaagtcc tgtactgatt gtagatgata
    28921 ccgtttttga aaaagcaaaa ggtgtggatt atcaagtagt caatgtagat gtatcaggtg
    28981 gaaataatga aaaaatagat gaacgactga gcaatgacct gtcgataaag tggctcaatg
    29041 ctgtttatta ttcttatgat atcgtaaatg gaagtctaca aggagaaatt agcacaaaga
    29101 aacttccgga tgaccaaatg aaggaagcac aatttagcca agaaagctct cgtttgaatt
    29161 tctcttccag ctattcgaaa ttgcgtgcag ttaatcggcg aatcggtatc aatatttttg
    29221 tcacactatt tgtcgggatg atcgtgatta ttgctactgg tagtattttg acagtccggc
    29281 aattagcaga agcagaggaa gaaaaagaaa attatcacct attgactaag ctggggattc
    29341 cacaaagaag gatcagacgg atgatcttcg agcaaaacgc acttaccttc tttccaccca
    29401 tgatattagg gatcactcac gcagtatttg caatcaatgt cttcactcag tatataaaaa
    29461 ctgccgatta ctggttggct tatcttgtga gtggcttatt gatccttatc tatcttttgc
    29521 tttatgtcat tacgagtcag ttatattgtc ggattatcga ggaatgatat acggaataag
    29581 acaagataat aaatagaact aaattttgaa taccatttat t
//
LOCUS       DACDHL010000024        29269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDHL010000024 DACDHL010000000
VERSION     DACDHL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.024"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..312)
                     /locus_tag="IV228_001145"
     CDS             complement(<1..312)
                     /locus_tag="IV228_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717078.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YE"
     gene            434..1018
                     /locus_tag="IV228_001146"
     CDS             434..1018
                     /locus_tag="IV228_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717079.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1029..1274
                     /locus_tag="IV228_001147"
     CDS             1029..1274
                     /locus_tag="IV228_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717080.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1280..2002
                     /locus_tag="IV228_001148"
     CDS             1280..2002
                     /locus_tag="IV228_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717081.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            2133..2876
                     /locus_tag="IV228_001149"
     CDS             2133..2876
                     /locus_tag="IV228_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717082.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2882..3247
                     /locus_tag="IV228_001150"
     CDS             2882..3247
                     /locus_tag="IV228_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717083.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3263..3793
                     /locus_tag="IV228_001151"
     CDS             3263..3793
                     /locus_tag="IV228_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717084.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            complement(4389..4868)
                     /locus_tag="IV228_001152"
     CDS             complement(4389..4868)
                     /locus_tag="IV228_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717085.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            4988..6325
                     /locus_tag="IV228_001153"
     CDS             4988..6325
                     /locus_tag="IV228_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8717086.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DRDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            6348..6875
                     /locus_tag="IV228_001154"
     CDS             6348..6875
                     /locus_tag="IV228_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717087.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            6862..7425
                     /locus_tag="IV228_001155"
     CDS             6862..7425
                     /locus_tag="IV228_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717088.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            7848..8585
                     /locus_tag="IV228_001156"
     CDS             7848..8585
                     /locus_tag="IV228_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717089.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(8602..9018)
                     /locus_tag="IV228_001157"
     CDS             complement(8602..9018)
                     /locus_tag="IV228_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717090.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            9410..10657
                     /locus_tag="IV228_001158"
     CDS             9410..10657
                     /locus_tag="IV228_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAP8717091.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            10927..13317
                     /locus_tag="IV228_001159"
     CDS             10927..13317
                     /locus_tag="IV228_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP8717092.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            13366..15201
                     /locus_tag="IV228_001160"
     CDS             13366..15201
                     /locus_tag="IV228_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8717093.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            15276..16133
                     /locus_tag="IV228_001161"
     CDS             15276..16133
                     /locus_tag="IV228_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8717094.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            16123..17232
                     /locus_tag="IV228_001162"
     CDS             16123..17232
                     /locus_tag="IV228_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8717095.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            17571..18371
                     /locus_tag="IV228_001163"
     CDS             17571..18371
                     /locus_tag="IV228_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717096.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            18459..19265
                     /locus_tag="IV228_001164"
     CDS             18459..19265
                     /locus_tag="IV228_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717097.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(19336..20637)
                     /locus_tag="IV228_001165"
     CDS             complement(19336..20637)
                     /locus_tag="IV228_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8717098.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            20998..21867
                     /locus_tag="IV228_001166"
     CDS             20998..21867
                     /locus_tag="IV228_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8717099.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            21887..22234
                     /locus_tag="IV228_001167"
     CDS             21887..22234
                     /locus_tag="IV228_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717100.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            22247..23509
                     /locus_tag="IV228_001168"
     CDS             22247..23509
                     /locus_tag="IV228_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717101.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            23589..24965
                     /locus_tag="IV228_001169"
     CDS             23589..24965
                     /locus_tag="IV228_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8717102.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            24962..25591
                     /locus_tag="IV228_001170"
     CDS             24962..25591
                     /locus_tag="IV228_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717103.1"
                     /translation="MIIVSSFLRWFTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            25610..26089
                     /locus_tag="IV228_001171"
     CDS             25610..26089
                     /locus_tag="IV228_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717104.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            26591..26644
                     /locus_tag="IV228_001172"
                     /pseudo
     CDS             26591..26644
                     /locus_tag="IV228_001172"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26648..27247
                     /locus_tag="IV228_001173"
     CDS             26648..27247
                     /locus_tag="IV228_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8717105.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            27263..27739
                     /locus_tag="IV228_001174"
     CDS             27263..27739
                     /locus_tag="IV228_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717106.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            27756..28658
                     /locus_tag="IV228_001175"
     CDS             27756..28658
                     /locus_tag="IV228_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717107.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            28731..28925
                     /locus_tag="IV228_001176"
     CDS             28731..28925
                     /locus_tag="IV228_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717108.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            29030..>29269
                     /locus_tag="IV228_001177"
     CDS             29030..>29269
                     /locus_tag="IV228_001177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717109.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHE"
BASE COUNT        10882 a         4170 c         5796 g         8421 t
ORIGIN      
        1 ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta gtagttccat
       61 ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt ctaaaatttt
      121 cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa ttaaatctaa
      181 aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat aaaaactact
      241 tctaacttct tctaataatg gaataattct aggattttta acttttttta tttcatcatc
      301 taatataaac atataaatct ccttttttta tttgtatgac aagtttacca taaaaaaaaa
      361 ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat atgtagagtg
      421 agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc ctgataatcc
      481 tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat tttatctaat
      541 tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt tcgatataga
      601 aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag attgggaaga
      661 aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac aagatgctga
      721 tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat tttatcttat
      781 gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa aaacaatcaa
      841 ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat ataaagatgg
      901 gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg aacttaggga
      961 atatgtgcag aacccatata attttcaata tttagcagga agaataaatt tagtttagga
     1021 gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt gaaggtgtta
     1081 ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc attcaaaaaa
     1141 atccacaata cctattattt agtctttatg cttggattgt acaaatggtt tgggtttcca
     1201 tattgatgct gttgttttat tggatagtac gggttattct tttattgtta aacaaaagaa
     1261 aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt tatcgacagc
     1321 agcattaata acccttacag catgtggtac aactaatagt gctaataata acaaagacac
     1381 aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa caacaatgtc
     1441 ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta ataaaggtca
     1501 aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg aaaattatgg
     1561 gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg attgtccaga
     1621 ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta gttatgctcc
     1681 aacattaatg catgatatta ccataaataa accagcttac gagtattacg cagttgctgt
     1741 agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg ctgatgagaa
     1801 caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta acggcacact
     1861 atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg aaagtacaac
     1921 taaatattat atggatttgg gagcatataa attatatcaa caatcagagc ctgttatgga
     1981 cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt aaaattaagt
     2041 gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga aacacacaag
     2101 ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca aatgaagctc
     2161 atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa ggaatttata
     2221 aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta aaggcaacaa
     2281 gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca agtgaagaaa
     2341 tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa aaacatctac
     2401 tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa aaattaagaa
     2461 ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc ttaaatgact
     2521 acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac acacaataca
     2581 aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgctctg tatgagatga
     2641 aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg aatcaagcga
     2701 ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg atcgtaaatg
     2761 aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc ttgaagtcat
     2821 caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg agatgagtta
     2881 gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa aaatacagat
     2941 gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg atcatattgc
     3001 taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga tacatcacaa
     3061 tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat atgatctatt
     3121 agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg cagagttaaa
     3181 aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga taatgcaacg
     3241 aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac aattaataga
     3301 agaagattta gagaccacag ttcagagatt agcggaacga atccattttg caaattatca
     3361 aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa tgaaattttt
     3421 ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga agaccatgat
     3481 ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag aggcaaaaga
     3541 aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga aatgcgttga
     3601 tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa tagattttat
     3661 tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag atcatgaaca
     3721 gttgtttatg agttttaaaa aacaattgaa agaaatacaa aaaagatata acaaggtgtt
     3781 tcctaatgcg tgaaaagctc aaagaaatca gagttttttg gataattaac aaagaatgta
     3841 tcatcttata tactcttgga tttcttagca tgatcttgtt ttggtggtta tatttaaaac
     3901 agtaattgga gagggtcaag aggattttta tcaagataag gatgtggaaa ctgcatgtgg
     3961 aataatagat taaaaacagg attgttatta atagttattt cctgcgctat gatgattggt
     4021 atgaggatac aaagggaaca atcctatttt gaagtttctg ctaacaacgt aatagaaaaa
     4081 tgctattacg gtcaacatta ttggagtgag gaagtcagag aaaatataga tcgtgaatat
     4141 gttcaaagaa ttgttagatt accagtaagt gaactaaaaa aatgatgaga ttatcagcat
     4201 atcttttaat gtttttgaat gtggtcacgt taatctttgt agtaatcggt gtgactttcg
     4261 atttaatgga atagtgggaa atctataatg gaaaaaagag acacgaatga acatattttt
     4321 tgaatcaata ttgttgatgg tttttgcgat cagcgcttac cagaaatcta aaaaagagta
     4381 atgattgttt aatcatcact ctttttttgt agggaaattg aaatatgtgg agcttgtatt
     4441 ggtttagtta ggattgcttg gctataagga gctagttctt tatcaaaacc atcaaagtag
     4501 gttttcagta gcgatttaac tgatcgctca tcttgtttga tttggataat ttcagcatcc
     4561 aaatcggttg tgagatcagg agcttttgat gcgattacct gatttttttt atcttccaac
     4621 tctcttaggc tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat tccaacgtga
     4681 gttgcaattt taacagaagt ttcgtccgta ttgtcatcaa aagaactcat tggaatatca
     4741 agtagtaacg aggatgtttc ttttactgac tttttcttta cagcattttt tgtttttcca
     4801 tcttttttgc tctttactcg agatgttttt ggagctagtt taggtaaatc acttgtttta
     4861 tcagtcattt attagctttt cctttctatt aatttatgtt attatattat cataataaca
     4921 taaaaaagta cactttttgg gggcgtagtt attgaaaaaa tctaagagaa agattgccac
     4981 attgcttatg ttaatggtgc aatttacagg gattgcaatg agtgtaatca caataaatcc
     5041 gcaagtgata catgctgatg ggatagctac ttatgatgca caatttaact gtactactga
     5101 aggtagtggg tcaggaacag atagggatgg aaacgatact ggaagtggcg ctgtaacagg
     5161 tgatgtagcc aaaaatattg aagatattta caaatacatg aatggagaat atggcttatc
     5221 aggtgctagt attgcaggta ttttaggtaa ctggaaacaa gaatcaggta ttgatcctcg
     5281 agcagtagaa gggtattacg gagatggaca gagagaagtt gacttcatca aacaatttac
     5341 tgatggtctg atgggtattg gctatggtca atggacagct gggcgacata cagcattagt
     5401 tgattggtct aagaaaaata atggcggttc ttggtggact acagaagccc aattggactt
     5461 tatgttcaaa ggtgatgggg gattcgtagg aatattaaaa gattatgctt taaatgcttc
     5521 tgatgatgtc gtagaaaatg cagtaacttt tcataaaaac tgggaaattt cagctgattc
     5581 cgaatcagct gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga agtacatgaa
     5641 aagcaagggt atggacggtg caaaagatga aagtaagatc aataaaatat ccggaggttc
     5701 aagtaccgga gatggatcga ataattcatc aagttcagca tcaggaacag tagaagatgc
     5761 ctgtgcaact acggatgacg ctaattctgg ttctggatca attgggaaag aaggaaaaat
     5821 taacggtaaa aaaggtcaag taatagcgaa tatgacttat gaagaagctt tgaaaaaata
     5881 tgggaaagcg atcacgctac caaaattcaa agaaccagat tggtcaactt caccttttaa
     5941 cggaatatat caaggtcagt gtacagagtt gacgtgggct tatatgagcc aattatacga
     6001 tgggaatcaa ccgactactg gaaatggtgg ttatgtatgg gaaagctaca aatcggcagg
     6061 agcaaaaata acgaacaaac caatcgtagg ttatgggttt agtgcttcag atggatggtt
     6121 atgggctgtt ggcgatgcag ggcatactgg tgtagttata gcggtgtttg aagatgggtc
     6181 atttctatgt gctaacttta atgttccacc aacccaagct ccaacaaggg gagtttcggt
     6241 aactttgatt gatggagtgg acggatcaga taatgtgcat ttctttagcg gtgtaggaaa
     6301 agctaaattt gaaatcgaaa aataaattgg aatgaaggac gatatagatg aatagtaaag
     6361 tgaaagatac aattatcatt tctttgcttt ctggtatgtt attagcactg cttgttggaa
     6421 tcctattctt caataagcag aatcaggaca tagaagctga aaaacacagt gtgcatattg
     6481 aagataagct gacattcttt aaagaggtta aaccaaatga ggtggatgga ttacggaaag
     6541 atttagaaaa tgatattcaa gaaatgttga atggatcagt tcaaaataag tttgcagatc
     6601 aatcaggtga agaatttttg agaaaactat ttattgtaac aggtaaacca ccaattactg
     6661 ataagagtag tgagaaagaa aaaatcgagt actataagcc ttttactttt aagctaacaa
     6721 actttggaat taagacatct agtagtgggt tgtatgaagc attgaccgat tttgaagttc
     6781 aatacaatgg aaaaaaagtg tatgatacca cattaaccat cacactaaat aatgataaga
     6841 aagtgactgg gggtgctttt tatgcaaaat catgaaatca ctactgctaa aaaggcgatc
     6901 ggaatacttt ccataattgt gatcctttgt tgtgtggtac tatttcaaaa gaaaaatgct
     6961 tatgataata atgtcaaaga attggaagac cttaaaagta aacaaatcga agtagtcaag
     7021 aataaccaga aagtgaaagc tatgcaggaa gctcaataca aagaattggg gaatgctgaa
     7081 atcaaggaac aaagtaggaa attctttaat ttattttaca actggaattc atgggaaaaa
     7141 tatacgaaga atatgaagga aatacaaaaa gaatttcctc agatagtaaa gtctagtcct
     7201 gttgatattt ctggcacgat ggtaggcact ggtaatagtc caatatctag ctatactgct
     7261 gattactata caacaaatca aaaaggtaaa atggttgaat tgattacaca gacaagaaat
     7321 actccaacaa cagcaacaac aacaatttgg cgtggagtaa caagcatagg agaaaatcaa
     7381 ttgttttact tggaggattt agatagctat gtaagagcgg agtaaaataa aaaaagagct
     7441 aacggtacca acgttagctc tttgtgctga cgatgataac aaaacctacc gaaaaaggat
     7501 tgtttcatca caaacacaaa cagaaaagaa ttgataaaaa acccgaacct cagaattttt
     7561 attaatcaat tcatttagat tataataaat tgagctgaac attacaatac gtatatgttg
     7621 taaaacgtaa aaaaagtcaa aagtaaatac tttcttaaaa agtcaaataa aactattaaa
     7681 gacaatatat atggtttatt tgacataaga aaatggtcta gatgataatt gtaatataga
     7741 attgtaggct taaataaagc ataaaatacc ttttatgata aggtatagct cttaatttta
     7801 agcagttttt agatataaga gtgaaagtaa agtgggggag aataataatg gcagatcgta
     7861 aattagaaaa gttattagaa gaaacgtgga atccaaaaga atttagtgaa ttttttatgg
     7921 agaattttga aactgactta gcagtgattg ttaaagatgc tcttagagag caagggtatc
     7981 ctgaaactgc aaactatata aacattaatt ttactttgta tactgaaaat aaaggaactt
     8041 gggatttttg ggctacttta gcaaataagg agttaagtga taaatcagac acaggaattc
     8101 gtaacttttt tgaatcaaac agagatgatt atatgtatgc taaccatcaa gataaattga
     8161 actttagagt agagtttgat gaaacacctg aagaatttat agaaagacaa ccgcctaaag
     8221 aaaatgtagc aaaggtattg gaagatcgtt ggaattcaga tgaaatagtt tcaacaatta
     8281 gcgaactagg cggacaatat gaaccgctag tagaagcggt tagagaagag ctaagactga
     8341 ataagtttcc tgatgttcaa aatattgatg tttctcaaat tgaaataaac gtcaagatca
     8401 cgaataaact tgattatggt agttgggcag atatagcatt agaaaaatac atctactcaa
     8461 cgttaaaaga gtttatcgaa aatagaatgg atatcatgta tctacagcat ccacagtatt
     8521 taaactttgg tgttgaaata gctacacctc ttgaagaatg gaaaatggag caagggcttg
     8581 actagccttt gctccatttt tttattttac aaatgaatac agttcgtcgt ttttaccaag
     8641 aatgattgtt tgcccaactt tgagattgtc aggtgatcct tcagcggttt gtaaggagaa
     8701 cgttccgttg aacttatcgg ataccgttat aggtagcttt aattctgatc cttcctcatc
     8761 tttgagaatt gctgtttttc gttcatcaaa ataaacagtc tttattaaat aatttttcgt
     8821 agtgatctca gcagatttgt ctttttttgt agtagtaaca gtttctttag aattagaggt
     8881 attttctacg actattccat ctttggcatt tgagcttgtg tttatttgat tgaatatttt
     8941 cagcgacaag ataccgctaa gaatagtcaa tatagaaaca agtgtaatta atactgataa
     9001 tttccttttt ttgttcattt tttcttaact tcctttttgt tttgattagt tagatattcc
     9061 ttatctaaca agccttttta ggctattaaa tgaagataaa ccccaaaaaa atattgcaat
     9121 tatgacagtg caatggtact attattatac aataaatttt gcgaaagggg taggtctaaa
     9181 tcgcaaacaa aaagaaattg aacctagaca aatacaactt gtacaccgca agggaagcaa
     9241 gtttgatttt gggtaaaggt agaaattatg ttacaaacat aatcaactat tatccaaatc
     9301 gtttgcctaa aggttcgttc aagaaagtgg gtgacaaaac ctatattata acggaagaag
     9361 gaatagaagc attgcgatca caaacagaaa ataaaaacaa aaagaaggaa tgaatatgaa
     9421 gaaaaattcg cttggtatgg ttttatttgc atcagctgta cttgcggggt tatcagtacc
     9481 agtaacctca catgctgatg aatggactgc aaacacaccg gatcaaatcc aaattacaac
     9541 aggacaatca agttatgcgt tcaaaaaagg agatactatt tggtcaactg tagagaagct
     9601 taaaagctta ggttacaaga ctactgttgc tgagtttgcc gaattgaaca atgttgattt
     9661 ggcaaaagga gaacaacgga ctatcccaat tggacgaact ttcaacatca gtaaagatac
     9721 attaacagtt aaggaagcag atggcgaagt agttagtcaa actgttcaaa aagataaacc
     9781 tgatcctgtt gaaaatacgg agcaagagaa caaaaaatcc gagactgcaa aagaatcgga
     9841 accaacagca aacacttctg ataagacaga aacaagtgct tcaagtgttg atggagcaag
     9901 ttcatctaat acaaataatt ctacaagtga gaaagaagca tcacagggaa caagtgaaag
     9961 tagcactacg gaaactgata ctggaacatc aacaaataac tcacaatcaa catcaaatga
    10021 tgcaaatgaa tcaaccagta catcttctag tgatcaatca caaacaggtt cgagtgatga
    10081 aactgacagt tctacttcac caagtaaacc cgaacaacca actaatgttg tgctaggcgt
    10141 tccatttatt agtcaaggta acacaatgct ttgcgaagga acatctttgc tagaagcgtt
    10201 acattataaa ggtgttgcaa caaatcaaga tttaatgact tttgttaaat caatgccact
    10261 atctcctgat aataatccaa ataatggttt ttctggtgaa tggagacaca atgtggatgg
    10321 aacttatcaa ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta atggcggaaa
    10381 tgttgtagat atttctggtt catcggtaca aggattaaaa gatgagattg taaatggaaa
    10441 tcctgtagta gcgtgggtaa cttatgcctt tgaacctgct caacataaat caatgttttg
    10501 gggcgatgct atttggaatc gacatgtagt agccgttgat ggatttaaag acggttttta
    10561 ccatgttgtc gatcctgtgt ttggggcaag ttggattaat gcaggatcat ttgaaacagc
    10621 atataacgta tctcaattag cggtttcggt acgttaataa aagagattgt ttatatacta
    10681 agtagttaag tatagtcttt atttgtcctt gcttaactac ttagaaaact tgttgctcat
    10741 ggtgggtaga actaaaaggg gtgatgctat gaatactaga gcgttgaaac agcttgcttt
    10801 caacgaaaaa agtgattaat aggtgtggtg attttaactt aggaatgggc gcgaaggtca
    10861 ttaccacacc gacatatcgt ggctcatttt cttcgcctaa ctggtataaa gaaggagtaa
    10921 cgagagatga agcatgaaaa aacgataatt ttaaaatcaa aaataattta ttgttttgcg
    10981 actttagtaa tggtgttatc tacgcttata atgccttttt cagcaataag agctgaagca
    11041 gatgtcacaa aaccgacaag tgcaaaattt acagaaactg gcgtaaaaac agacggttat
    11101 attagggtta atatgccgaa tcatccaaat gaatggatga tttcaagtca atttaaagat
    11161 tctcatggaa atattggata ttgtatggat tcagaattgc catccccaac aggaagtgga
    11221 gccggaagct tgaagtacaa gggagctggc agtgatgagt tctatcgtat gtttaaaggt
    11281 ggttttccat caaaaacagc aaaggaactg ggtgcaggaa atgatacaga agcatggtat
    11341 gcaacacaat tagtttcatg ggttttagcc ggaaacttta aagtttcaca gattgtatgg
    11401 tcacatccaa accataccgc cgcagaaaca gctcgtgtaa aaaaagcatt tgaaaaaatt
    11461 tatgactatg ctaagaatgg aaaagatacg cccaacactg agttttctat cacagcttct
    11521 aagacagctg acgaaggaaa atatcacact ttcacatata aaacagcatc aaataagact
    11581 ggtaatgcga agctcacttt tacatcagca aaaccagctg gtatgaagat ttatgatgca
    11641 gatggtaaag aaattactaa caacactgtg aaattaaatt caagttttac tattaaagta
    11701 cctgtgacta caccaagtgg tactttgtca tttaaaggta ctgctaatgt tagcacaacc
    11761 aatcctttta cttttgatgg acgaggcgtg tatcaagatg ccgtagttat gatcactact
    11821 tcagaaacaa aggactctaa atctttgagt gctaaatgga caagagctgt tggtcaggta
    11881 acagttaaga aagttgatca agatggaaat gctttaaaag gagcggagtt cactctaact
    11941 gacaataaca atgctaaatc tgttaagaca accggagatg atgggttagc tacgtttgat
    12001 ttgcaacaag gcttaacata cagcttagaa gaaacaaaag ttcctgaagg ttatcaaggt
    12061 tcatttaaaa aggatggtat tactgttaat accgatggac aaaaatttag ttacactgct
    12121 acgaacacaa ttaaaaaagg taaagtaaaa gttaaaaagg ttgaccaaga tggaaaacca
    12181 ttagctaatg ctgaatttac cttgacggat gattcatccc aaacacaagc agaaaaaaca
    12241 gatgaaaacg gacttgcaag cttcgatatt gtagcaaaca agacctacag ccttgaagag
    12301 accggagtac caactggtta tacaggcgca ttcaagcaag aaggaattac cgttaaagat
    12361 gatggacaaa cttttgaata tacagctaca aacactttga aaaaaggtgt agtaaaagtt
    12421 cataaagtcg atcaagatgg aaaacctttg gcaaatgcag agttcacttt aaccgatgaa
    12481 aaaggtgcaa cacaagtaga aaaaacagat gaaaatggac ttgcaagctt caatattgta
    12541 gcaaacaaga cgtatagtct agaagaaacc ggagtaccag taggttacac aggtgcattt
    12601 aagcaagagg gtatcagtgt taaagacgat gggcaaactt ttgaatatac agctacaaat
    12661 aatttgaaaa aaggtgtagt aaaagttcat aaagtcgatc aagatggaaa acctttagca
    12721 gatgcagaat tcaccttaac ggatgaaaaa ggtgtaaaag aagttaaaaa gacggatgca
    12781 actggtctgg ctacgttcaa cattattgcg aacaaaactt acaacttaga agaaacaaaa
    12841 gtaccaacag gttacacagg cgcattcaaa caagaaggaa ttaccgtcaa agatgatgga
    12901 caagtctttg aatatacagc taaaaacact ttgaaaaaag gtgtgatcaa agttcataaa
    12961 gtcgatgatc aagggaaagc attagcaaac gcagaattca ctttgacgga cgacaaaggt
    13021 gtaaaagttg tacaaaaaac ggacagaaat ggcttggcaa catttaatat tgttgccaac
    13081 aaaacgtaca aaatcgaaga aacacaaaac cctaatggtt atacagggaa atggtctaaa
    13141 gaaggcatca ccgttgctga cgatggacaa acctttgaat atacagtaat taatcacctt
    13201 gtacctgaaa cagtaagaac aggcggaatc ggtacatcag ccttccttct tgtatttcta
    13261 attgcaggat tgagtatggt gctactgtat tatcgagaac agcacaaagc aaaataaaaa
    13321 agtgttaatg gaaaagaagt aaatatgaaa aaagaaggct gacgtatgaa atcaaaaaaa
    13381 attttttcaa atttaatgct atttggtact ttgctaggag tgggattggg cgcaagctca
    13441 atctcttcta gccttggact aggggggcaa gtagcatatg cacaacaaaa ctataattat
    13501 caaggaactg gaacgattca tatccataaa gtaacaaata ctaatgggga tgggaaccct
    13561 gtctcagata ttgataacaa agggaaagaa atagctttac caaatggtgt aaaacctctt
    13621 gaaggcgttg agttctctta tgtgaaactt actaaagatg aagctaaatc cttgggatgg
    13681 acggatagtg atactacaaa aggtcaacat attgatgatg ctaaagcacg attattacta
    13741 aacaaaaaat acagtgaagt aagtagaatg aagactgatt cacaagggaa tatagacata
    13801 cctatacaag taaatacaga cgatttaaca gataacttgt atttgattgt tgaaacagga
    13861 acagtcaaag gtgtagttga agaagcgtat cctatgattg ttaacgtacc tgaactaaac
    13921 ggatcagatg atcaatactt taattatgat gtacatgtct atcctaaaaa ctatttccaa
    13981 ttaaactcag cagagcttga taaaaaagac gaagatggaa aggctcttgc cggaactgtt
    14041 tttgaattgc gaaaagatga tggatctgat caggttgttg gaacttataa aacagacagc
    14101 acaggtaaat taactgtgga tggtttggaa ttaggtgact attatttcct agaaaccgat
    14161 ttgggaacag atgctaatag aacaacctat ttactagatg gtaaaaaatt caaatttagc
    14221 gttaaacgtg gggagactgc taaagtagtt cttgatgtta ccaactacaa acgacctgta
    14281 attactaagg taattgataa agaaaccgtt gatactggcg aagatgttca atatacattg
    14341 aaaccatcaa ttcctggtaa tattggtaat tacgaaaaat atgttgtcaa agatgttcta
    14401 ggtgcagagt taacttatga ttcatcagaa gtgatcgaag gaatgacttt agatactgat
    14461 tacagtatcg aacgtgacgg acaaacgaca aactatgtct ttactgaatc aggtatggat
    14521 aagcttcaga aacaatttga tgcaggggat cgcttcttta caattttgat gaacgctcat
    14581 actaatagta aagctaaaat ggatacacca cttaagaatg atgtttcaac gatctacaac
    14641 aatggatatg aacccgaaga cgaaacacca gttgatcacg cttatacaat cattggtgat
    14701 caccaattca ttaaaacaga cggagataca agcaaagcat tgagtggagc aaaatttgta
    14761 gtgaagaaca aaattgatcc gaataaagtt acttacctaa aacaagatcc aacaacgaaa
    14821 gctgttactt ggattgcaga taaatctaaa gctactgaat tcgtttccgg atcagacggt
    14881 aagttccaaa ttgaaggttt gcaatatacc tatgatgtag attttgataa acaaggtaat
    14941 ccaacaatca cagataaaaa aatcaatgat tatgctcttg aagaagttgt tgctccttca
    15001 ggatatacac caactagaaa cgatgttcca tttgtggtat cgaatgatga tgcttctagc
    15061 gaagtaacag acatcaagaa ccttaaaaca cctgaaacag ttcgtacagg tggaattgga
    15121 acagtagtat ttctagtggt tgcaggtgtg gcaggaacag ggctatatgt tgtctacaaa
    15181 cgagaacaac acaaagctta atgaaataaa attccctaga gtgtttttgg ctctagggtt
    15241 tctttttaaa aagtgtgtga aaagtaggta tgagcatgaa aaagaagaat atcaagttga
    15301 tcatcttaaa aattgggatg ttgctatgtt taggagtagg tctatatcct gtaattactc
    15361 aggcttatac ctactaccaa actcaacaaa cactaaactc ctataaggaa aaatatgatg
    15421 aagcttctga aaaggagaat gaagaagtct tcaagagata ttcagaagtt aataggaaac
    15481 gaaaagacac agaacaaacc tcagtcacga ttcctgaaga taataacaat attccgtcaa
    15541 atggaaatct tcagaaggat aaattaggtg aattagtggg tgaaattctt ataccaaaga
    15601 ttaatgtgga tttgccaatt tatgatggtg taaacgattt ccaactatca gcaggagcag
    15661 gaatattaac cggaactgat tatccaactg gtgggattgg aaaccattct gttatatcag
    15721 ctcattcagg cattcctaat aaaaaatatt ttacagattt agagttgctc aaacttggtg
    15781 ataaattttt tattgatatt tcagggaaaa aacttgctta tcaagtagat cggataaaaa
    15841 caattctacc aaccgaattt gaggatttgc ttcctgtggc aggaaaagat tatgtaacac
    15901 ttatgacatg tacacctgtt ggagataatt ctcataggtt actcgttaga ggggttagca
    15961 taccttacca aaaaagtatt gttgagaaag aaaagaagaa agaagattta aaggtaaagg
    16021 cgcagatgaa ctggtatcct atcgtagttg taggggtttt attaataata gctgtgatag
    16081 tcattattaa aaaaatagta gcaaagagac gaggaatgaa aaatgaagaa taaaatacta
    16141 ctttttttga ctcttatttg cactttggtt ttaacaattt atcctgcaaa tcagctctat
    16201 catcaattca aaacaaaaga gaatgtggcg gaaatccaag agaaaattga gaaaaaacaa
    16261 actccttcaa aagaggaagt ggcaaaaaaa gtcgaacaaa taaaatcgaa cagcggtaac
    16321 caaggattag acaatactca gaaaacagat aaaggagtag acaatactca ggaaacagac
    16381 ggtggatcat ttgatattcg tgaagctgaa ggaaaggtca atccatacac aggagatgtt
    16441 ttttcaaaaa atcaaacgaa agattttgaa ccatctacct attcaggagc ggttggttat
    16501 gtctatattc caaaaataaa tgtgctagag cttttgtttc taggcgcttc acaggagcat
    16561 ttatatagag gtgtggcaca aatgagtgct actagtctac ctttatcagg taaaggtcat
    16621 caaacagtaa tagcaggtca tcgtggcggt tatacgtcac cacaatttct ttatgttgat
    16681 caattagtag ccggagacca catttatata gattatcttg gtgaaaaagc agtttatacg
    16741 gttttccgaa cagacgttat tagaaattac ggtcagaatt caacactgac tgatattcct
    16801 gatttagata tgttaacact ttatacttgt catccatatc ctcagaacta cgatcgttta
    16861 ttagttagaa gtctaagaga tgaaaaagca aaatacatca cgccacaagt aacagttggc
    16921 aataccgcat acgctgatgc cattgatagt gaatccgttt cagataagcc agaaactgtt
    16981 acatcttcta atgatttatt tgaggatgtt gatcgtgcca acaaggaatt agatggaaat
    17041 aatgatctta atattccgga acaaggtagc gtagagatgc aagacagaac gtttgctgaa
    17101 aaggcaagtt caatatttac aggaattacc aataaacatc agaaaatgct tttagtcatt
    17161 tggactgtat taggagcatt agcaagtcta aaggttctta ttcttttaat caaagagttt
    17221 agaaaagcat aatgaagatt ataaaaattg atagagtaca gtaaaaaggg attgatacta
    17281 ttattgatct cttttttatt ttgaatagag ctagattcac ttttaaataa aataaaaact
    17341 gatcttaatt ccaaagaaaa agtcaagaaa aaaatacaac aaattttttt atcagaatat
    17401 ttatcagaat attattagaa aagataaact caaaagctta atttaatagg gttattggtt
    17461 gtatttagaa ataatgtatg gaaaagctat aaagcaatag ataagatgta gaaaatctta
    17521 tttgtgaatg aaaaacaagt catcgtaaaa tattaatagg aggtgtagta atggtggaaa
    17581 tatcaaaaac agctttagac tcattagcta aactatttag tagcaggttt gatggtgcag
    17641 aatttgttga atatctaagc caaaacgaaa gtggaaaaac tctgatcgaa acactgaaaa
    17701 aagactattt gaatgaaaat gactttccga catggacgaa tattgaagat gctacatggg
    17761 atgtagtgtg tacgatatat tctccttggg taacgattgc tgattttgtt ttagataaaa
    17821 ttggagggga tacataccct gatgccaaat ctcttgctac aagtgacttt aaaacgttct
    17881 atggttatct aatgggtcta ataggcaatg gaatagattt gaaagttacg ccaaaacaaa
    17941 cacctgaaga aattgaaaaa gaaaaaggag aggttcaaat ggcttatgca gatgacagag
    18001 aaaaagtaaa attattggaa ggtaaattat ctgaagaaga acttggaaaa atggtgcaag
    18061 aaatcaatga agacagcaac tattttcctg aattgaaggt atatcaaaac gacgaagatt
    18121 tctttaatga aatgtttagt aatagtccat gggaagcagt gagtaaaaca aatttaggag
    18181 aatggaactt tcaagatgat ctagttgcat ttagcaatga tgggtattta cgatcatatt
    18241 cagaaagtga gtatagagct gaattaaaaa cgtatgaaac ggatattctg atgagctatt
    18301 taaatctatt agagagtggt cagggagagt taagagataa agttgaagaa ctattaaaag
    18361 aagagctata gggattattt caacataaac acaagcagaa aactaccaaa caaaacgtac
    18421 catgcaaaaa gccggttaaa ccggcttttt gctctattat gagaggagat caaaccatga
    18481 gtgcaacaac cgatatggct ttagaagcag taataaaaga taatacagga aatgtcgaaa
    18541 atgtagaaga ctttagcgat attgcaatca atctttcagc atggatttca gctgtaggag
    18601 aagaaaatat cgaatttgag atcaagaata tggatgactt aaaagcaaaa tttcctttta
    18661 tcgcatcgaa actaagtcaa tttgccgagg agaaatcgtg gaatgaatta gttagttttt
    18721 ccgaaaaaat ttatgatatt acgcaagaag atgagtgtgt ttcatgggaa atgaataatc
    18781 aatacaagaa aattgatgct ttcgtcaaat cacgtataga aggaacagct tttgagtttg
    18841 aaggtataga taccgcagat aattggttaa ataaagaatc ctttattaaa agtatagcgg
    18901 atgatcttga aaaacaatgg tctaaggaag actatgcaga gtacctaaaa acagaaagag
    18961 atacagaggt gaaagaaaaa ctcactgagg tatgtttgga tgaacatatt gatcccaata
    19021 tcgtaaaaca agaagactat gaagtggtta ttgataaagt tcctactgat tttgaggaag
    19081 ttgtaaagta tatagttgaa gaagcattat cgcaatactc ttcagatgag gaagtggacg
    19141 aagaaactgt tcgtaccaga gtttggcaac tggtttttga tgacaaagat tataaatatt
    19201 tgagtgatca aaactcggtt aattggagtg tacgattatt aattgatcaa aatgatattg
    19261 aataaaatag gctctttgtc aaataggact gatagagtga gataagaaca tctaaggtag
    19321 aattcctttt ctactttaga tgttttttga ttcataggta atcagattat ttagtaagaa
    19381 aattctggtc ttcattttcc gaaagcttcg aaagccataa gcgactcgtt tcaatgcttt
    19441 gatgtgggta tgcagatttt ctattttggc atttgaataa ggcaattcga gggctaaacg
    19501 aatgccggat tcatagttta taaaagcttt ttttatagaa tgcagttctt ttggaacgga
    19561 attgggcatc gttcttacaa gtttaaagaa agtgtccgcc tctttgtcgt gaaacgcttg
    19621 aagaatttct tgatagtaaa aataggcgat tcgtaattcg tcagagatcg aaagcagacg
    19681 atccaccact tcactttcgg tgagatacgc ccaaccaaaa ttgcgccatt tggtccattt
    19741 gtcataaaca agcttgtcct gtcgtttgat cagaagccga gacaacgcct ttaattgtcg
    19801 ataatatttg gcttgtgttg gatcgtttcg atcaaagctt ttcatgattc gtacgcgaat
    19861 atcctcaaat ttacgaatga gatgtttgac aatgtgaaaa cgatccacaa cgagttttgc
    19921 ctttggaaaa cattccttta tcagagaggc ataagaaaca ttcatgtccg tcacgatgac
    19981 ctttaccttc aatcgtgcgc gtcgttcaaa ttgcataaaa taagaaacga gagccttttt
    20041 cttccgtgaa ggaagaatat ccagtaattt tccagtatcg ccatccatac aagaaaagga
    20101 gtagggaccg acttgatttt tcagtgaacg aaattcatcc attaataaac aacgcggtaa
    20161 ccaatttctt tttacttttg tcatggggat cagagaagcg agtgtccgag aaacggtcga
    20221 ccaagaaacg ccgcaatgct tagcaattgt cgtcattgct tgtttttcag ttagctcttc
    20281 taaaatttta tagcgcactt gtttagcgat aaagcagttt ctatcgacaa tgtaggtatt
    20341 cgccgtaaaa taggtgtggc agccacgaca gtggtagcgt tgtttcgcta aagcaagata
    20401 agtcagttgg ttcgcacatt tattaagttg aatgagcgat tttttctttc cgttcttaac
    20461 gatcatgcct tcatgattca tctgtttaca caaaggacag cgacgaattt ctttacttaa
    20521 cgtcgcagaa acaacaagac tcttgatatt cttaatgctc ttcgattcca catttgtgat
    20581 gaaaatgttt ttatctttga ttccaagtag tagtttgata tgatgaagat caggcataca
    20641 gaacgctcct ttgattgtgt tgtggtgatt caattctaac ggagcttctg tatgttttgt
    20701 tttttcatgc tcaaaaggga ccgatagaat gattttctat cagtcctaga aattatagag
    20761 ccataaaata gagctgaaaa gctctatttt ttttgataaa aaaactagct atttttttaa
    20821 aaataatttg gttcaaaatt tattttttta acttgataat tttttgccat ccttttttaa
    20881 attggctata tatcaaggtt tatagtgggg ataaataaaa atacatttga caaaatttac
    20941 aatatagttt attatatatt tatcttatta tgataacaca ataaaaggaa tgatggtatg
    21001 aaaaagaaga agtttagtcg atcgttgtta ggaattttag ttttagttgc ggttggtctt
    21061 agtggcaagg gttactttca gaataacagc actcaggaac atgattttca gatcaaaagt
    21121 agtgttcagg taggtaataa agcagacagt gaatcaaaag aatttttagg gatacgttat
    21181 gatccaaaca aatatccaaa taatatcgta aacattaatg atgggaaata tacactgaca
    21241 aaagaagacc atgaagcatt atcagaaaaa ggcagtcaaa ccttttgggt aaattatgca
    21301 gatttagatc aacttggaag agcaggagat gttgaagcgt tgatcaccta tgatgctata
    21361 aattctcact catctaaaaa tgtggaacgt ccatctttca aaagttctgt ccatattgca
    21421 ggagaataca aggatggagt atttgatcaa gagagagata cttgggtcgg caaagattca
    21481 aacaatgaaa tcactcagct agaaggatat aaaggttatc tctataacaa aagtcattca
    21541 ttagcatggt cgctaggagg agatatggag gtacacaatt taacacttgg aacgagagcg
    21601 caaaatgttg ggaaaaatga tcagaatgga gggatggctt actcagaaac gattgtacgt
    21661 gatgcaatct ataaaaataa gaatgaaaaa gtattgtata aagttgatcc aatttataaa
    21721 gggaatgaat tggttcctcg tggttctcat gtcgaagctt tctcagttaa cgataatgga
    21781 aaaacggtaa accttaatgt ttatgtattt aacaatcaaa aaggaatcaa catcaactat
    21841 caaaatggta cttgggtaag agattagaaa ttgagaaggt gaagaattgg aaacaataag
    21901 catcaatgga ttagataaat cagttgcaaa tcgaataaaa aaaggaaatg gctactatct
    21961 tctgaagaag ggtaaatatt cagttgctag agagttagaa acaatgagta tcgtgtatgt
    22021 agctaagaat cattttgacg tagaacaggc tatttttgat ccagattctg aaatagggaa
    22081 atgtgttgaa ttatcagaga aaactgttag tacaaaaggg gcgatgatca ttgagagaga
    22141 aatgttagac gaaatcagag taatcattcc taaagatacg cagatgtgtg aggacagcat
    22201 aagtgacgat gaaccaaaat cagaaagaaa ataaggagat attcagatga gcgaaaaatc
    22261 cattgaatta aaggaatata agcgaagcga tggagaaaaa tattatcctg tagccagttt
    22321 ttctaaatca gtatatctgg ttggacgtgc attggtagtt ggtgcaattg gcatatatat
    22381 tttgcattgg gtaacaaagg ttgtacaact gcttgtagat accttagtaa gtattcttgt
    22441 ctcgttaaat gatgttaacg gaacagatga tttaggatta aaagaaatgt ttaaagagtt
    22501 actcaatgcg ttttttccca attatttaag ttattcatat aatgtacaaa gcttttttgc
    22561 aaagctacac ttggtagaca tctctatttt agtattagaa gcaggaacgg tattctttgt
    22621 tggatatttg gttactttga tgttcaggca tcactatgga gaacaaagac cattttttga
    22681 tgatatggaa agtagaaggt tgaaaagaga agctctagaa gctatggatg tgaagtatga
    22741 tccaaacaag attataggag atgaaacgaa caacaaaaaa agaaaacgaa acaaaacgca
    22801 attagaatcc gatgtgctat caacgcttaa aaatatcaaa atttctgttc atacaagacg
    22861 tgatctaagt gtcaatgaag atattcgaga atatatcgta aaaatcccgc aaccaaaagg
    22921 gaagaaagtt agagaaacag ttttacgaca aattcaagat ttagggtcga tcctgacaag
    22981 agcaacaagc ggacgagcca aatttggtga aaaagaagat gaagtttcag gcgattattt
    23041 cattttccga gcatcagtaa atattgaaag agaacgtaag gaaagagtaa gtaccaaaaa
    23101 caaagaaacg gaaagagcaa catcttcaat cgcagtttca aattcacaat tttcttttcc
    23161 aatcaatctc tttgccgata attccaaaaa tatccaaaac caaaccgaaa aagctagaaa
    23221 atatgccgaa actcaaaagc aatctttaga actttatcta tcttctgctg acgtacctgc
    23281 aagtctaaca gatatgcacg ttgggaatac atctattgaa tatttgttta ctttatctcg
    23341 taccagaaaa attcaactaa gctcaatgaa agaagaaatt gaaacttact tgcatatatc
    23401 aggtgtaatc gtgcggatca acgcagggcg tattcaaatt actctaccat taggagaaaa
    23461 tgatcgtatt ccaattgatg taccatcaat gatcaatagc gtgttttaaa agacatcaca
    23521 aatagaaaca gaaaatacaa actaccagtt cacaaacaga aaagtaccga gaaaagtgag
    23581 gaatagaaat ggctatgaaa ttattagaga aagtcaaaaa gagagccgaa aaagctgaat
    23641 ctattttgct agataaagaa cctaatataa agcttaatgg aaaattcagt gtcaatatgt
    23701 tgaacacaaa ggctaaagac ccgacagatg ctatttttgg aattaagcct gatggcacaa
    23761 ttgctcattt ttgcatcagc gaaatgccac acatgttggt tgcaggaacg acaggatcag
    23821 ggaaatcagt attactgaat gaaattcttg ttacagcaat gtgtcactca acgcctgatg
    23881 aattaaaaat aggaattgta gacccaaaaa gggttgaatt tggaagatac aaaaaattgc
    23941 cgttcatgtt ggctgatcca attacagata tggatgaagc ttatgatttt tttgaatatc
    24001 ttgtgatctt gatgcatgaa cgttacaaac tgatggaaaa agcagggtta caaaacatca
    24061 cgctatataa cgaatatgcc gaaaaaaatg gattagatag atttccctat gtgatcttat
    24121 tagttgatga atattcacag ttggttggaa cacataaaga agtagaaggt ttgattgttc
    24181 aattaggaca aatggcaagg gcagcaggta ttcatgttat cttagctaca caatctcctc
    24241 gatcaacagt tgtcactggt attatcaaag ccaattttcc gtcacgtgtt tgccttatgg
    24301 tagcaagtga tttagaaagt aggatcgtat tagatgaagg cggtggagaa agcctaagtc
    24361 caaagggaga tatgattatt aagctcgtta atggaagtat ggttagagcg cagggagctt
    24421 acatcagtaa caaagaaatt gaaacaattt tcaatcacct tagaaatact atgccagaac
    24481 cagaatatgt tgattaccga gcaatcgtag ctgaatctcg tggtgagggt ggagaagacg
    24541 aggattttgg tggaattggt aaagctcctg taagaaaatc tgctaaaaga aatttgaaca
    24601 cacctttagc aaattcatca tctaaagagc catcaattga aaagaagagt acgtttgctc
    24661 taaaacttga aaaaaacaaa gaagaaaaag aaaaacaagg agctaaaaca ctagaaaaac
    24721 ttcaagccga aaaattagaa aaagttttaa aagcattaga taaagctgat tctgaaccgc
    24781 aaacctcaaa caacacgaat aatgaacagc cgatttcatc taaaactaaa ccgaaaacta
    24841 ctgatccgaa agtttcagta aacgtgacca tcacaaccga taagtctgag aagaaacaag
    24901 taaacggaac gacttcacct atcatgcaaa aaatgttgaa agccaaaaaa caggtgcata
    24961 aatgatcatt gtcagttctt ttttgaggtg gtttacgcca tataaatata cagatgaagt
    25021 agttgtgaaa cgaacgaaga aaagagataa aaaagcggta ctaactccta aacagatgct
    25081 tgatcatggc attttagggg tgaaggtttt agaagtgaat aaaccgtttg aatcaaatag
    25141 tgcaagagaa aagttgcttg gagctgaagc cgatgtcgca tatttttacc atgacaaaag
    25201 caagcatcaa atagctatta aattcaatcc aaaatcggca tggcggtatc aagcaagaga
    25261 tgacttgaaa cgtaggaaac ttagaccaat gctctatcca gaaggaaaag aagcatttga
    25321 tagaacccac atcgttccca ttggttttca tggtacagaa tcagataacc gcctattagt
    25381 aggttggaac agcaatcaaa atagagttca gatcaataaa tttgagaaga aagtaaggaa
    25441 attcaacgat cgaaaaacga tcctatggtt tgcagatatt tgtaaacagc cggatggaac
    25501 agcaaagtgg gaagcggtga tatttgacga aaatggtaaa aaagtaatgt ctgaaacatg
    25561 gcatgataaa gctaaatttg tttggcagta gcagtagagg tgaaaaagat tgctagaact
    25621 ttttgaagca aatagaacag gctttaatca aaagttagaa gtgggagaaa ctatagaata
    25681 taaagataaa aactatatta ttatcggaat ctataacacg agaattaaca gaataggtga
    25741 tccacgtata accagtgact tggtttgtca atctgttaac tattcacctg atctgacaag
    25801 caggtataag aaatacgtgt tattagaata tagacacaag attactgatg aaattggtgt
    25861 aaacaacaca cgaaacatat ttaagatcgg aacagtgatt ccttatagta acaacgaaga
    25921 aaaaatattt tatcagattt acggaataga aaagtttgaa tatgaacatg tcgatctgct
    25981 agtgacgtac caaatgcgtt taatagaacc ttggtcacag gcagaaattg ataaagcagt
    26041 aaaattgaac cgattatcaa aatttaacgt attaggaaat gccttttaaa aatcgcaata
    26101 cacaaacaga aaaataccaa aaaattagca atgaacgtag gtaagaacat gacaataaat
    26161 aataataacc ctaaaaaata taatgctctt gtccaatgct atatcgaaaa caaaattaca
    26221 atgcaagaag tcgctgatga attaggcaaa tcaaagagcc tagtatcaca cgattttaag
    26281 cacaatgttt ttccgaaccc gattgagctg aggaaaaaat ttcaaaagag agtttatcaa
    26341 gaaataggta agtggattga tgaagggaat gcaattgacc ctttaattga aaaatatcct
    26401 tttttaaaaa gcaaaaataa aattcaaagg ttaattcgtc aaggcgatat tcaagctaaa
    26461 gaacctgttt tgacaaccaa tgatttggct aaaataaatc ttcataaaca aattattgaa
    26521 gactttgaaa aaaatctttc aacaactgaa attttaaaaa aatacaatat tccgagagga
    26581 aagctaacat cgactctttt tagagaatat ggagaatcgg cagttactaa agcaaaggaa
    26641 gtagcgaatg agagaaaaaa acaaaaatag tagtcgatac ctagtagcga ctgtagtttt
    26701 tgtttgtgta ttgattttcc tgtttgattt tttgcagatt ggaaaaagca taattggaaa
    26761 tacattgagt gcaaaggaaa tggttgcttt aggtggtttg tatgaaggac acttgttccc
    26821 gaatatattc atttctatcg tttctcatat taacttgtac catttaattt ctaatttatt
    26881 gttcttagta gttcttgaag aagcactgaa tgatcagtat gagtggttaa cattattgat
    26941 cgttttaatc atatcaggat ttctaggaag cttagttcct tttctgctac acagcaacat
    27001 cgtaagtatg gggcttagtg gaggtatttt tggatggatt gctttatttg cgattgcata
    27061 catccgtttt gatagcaaga aacatttatt actaagtgtt tttgtattat tgataatcgg
    27121 cattttactg gtatctactt ttgaatcagg cgagacaaac gtcttgtcac atgtaatcgg
    27181 atttgtctgt gggatgatct tgggatttat tctaaagaac gtttcattga tcaaacaaaa
    27241 gaattaaaag ggagtgctaa aaatgccaaa aaaaaaagaa aagaaaattc cagtgttttt
    27301 gtctgtctca gaaatgttat cacttcaaat actgttaaga aaaaagattg aaacaataga
    27361 cgaagaagtt agaaataaaa gggctatgta cgaacaacgt gaagcgctaa gagaacgaat
    27421 ccactatttt gatgatatac acactaaaat ctatgaagca cgtacaaatc acgaagacga
    27481 aagcaaatta ggattagctc aacaacgaat atttgaatgg ctgaatacga atactgagca
    27541 agaaccgtta agagcaatta gtaatctttt tgaatttgga acgaagcatt ggtttaacgc
    27601 tgtagaaaag gatgctgact tacaagaagc gttcgataca ctaagcgagg atagcaaatc
    27661 catgcgagac ttattgatca cttatctaaa tgagcatgaa tttgaacaga cgaaagttaa
    27721 aaagaaaaat aaaaaataga aagttgtgat gtatcatgct gtttacctta ttaggttatt
    27781 tatgtttagt tcttgctttt tttggagcaa ttgctatatt tgtcggtcag tttcaagaaa
    27841 ataatccgat aatgcgtaac ataggcattg gaattgttat catatccttg attgtatttg
    27901 gtgccacaaa gatttttggg agtaatgatc agcagtttgg tatttctgga gttgataata
    27961 gttatgctcc agattcattg ggaagagtag tgatagatgg aacgtttgag aatgtaacga
    28021 gctacaaaaa tgtttcattg acagtgaaag aagatgacaa gaaagcatca gatgacgttc
    28081 tcgctacgat caagccgtct caatcacagt ttgaagtgtt ttatacagtc aaagagaata
    28141 accctgtacg caagctaaca ttagttttta acgttgacgg agaagaatac tataaagatg
    28201 taaaaatcaa taaagcatca acgaaaaaga ttgtcgaatc agcgaaaaaa gaagtagcta
    28261 cagaagaaag tacaactgaa tcaagttcta ctgtcgaagt tgaactacca gataaaattg
    28321 attttgatcc tgaaaaagtc aaatcgtttg aatctgtttc tggttctatg gcagatcggt
    28381 ttaacactga agttttcaaa gctatcaaaa ctgttaaatt ggatactgaa aagcaaagga
    28441 ttaatgttac cttattcaat gcagttgaat ctatggatca ggataaaaaa gttacggttg
    28501 caagagaact agggaaaaga gtaattggat tgtatcgagg tacggcaaaa ccagattcag
    28561 acggttcaga tttgccagtt cacgtagttt atgaagacgg taaagttttt ggagactcta
    28621 cagcaaatga agcactaacg attaaattag ctaaatagcc ggaaaattat aataaacaag
    28681 tggtgtcaca ccaccatcgc ataggtgtga caccttggag atgattactg atggtagtaa
    28741 ataaagaaaa agtaagaaag tatgttactt taaaaatagc ttctgttgaa tatttagaaa
    28801 agcgagctaa agaagaagaa aaaacaagag gtaagaaggt aacagtttca gagttgttag
    28861 aagaattaat tgagagtgac caaacaataa gacgtgcatt taccaatagg tctgttaaaa
    28921 gttagggtga tactatcttt aatttcaacc aaatttctaa tatatgatga atagtttcct
    28981 tgtacaaaat atctaattaa gagataaaag ttaggaggat tcattcaaaa tgattatcca
    29041 aaagtttgac tggaaaatga aattaggaat tagtcttgta atattgggat ttattatttt
    29101 tttggggata aaagcacaaa aaaatcaatc tacttttgaa actatgtctt ctcaaattat
    29161 tgaggaatgt tccaaaattt attattgtga gaacaaaact gaagaatcta atggctttta
    29221 ttatatcgat caaccacatg atgttgttat aaaaatggag tcacatgaa
//
LOCUS       DACDHL010000025        29102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDHL010000025 DACDHL010000000
VERSION     DACDHL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.025"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1034)
                     /locus_tag="IV228_001178"
     CDS             complement(<1..1034)
                     /locus_tag="IV228_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8717110.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFT"
     gene            complement(1116..1805)
                     /locus_tag="IV228_001179"
     CDS             complement(1116..1805)
                     /locus_tag="IV228_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8717111.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1911..2279)
                     /gene="rplL"
                     /locus_tag="IV228_001180"
     CDS             complement(1911..2279)
                     /gene="rplL"
                     /locus_tag="IV228_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8717112.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2332..2835)
                     /locus_tag="IV228_001181"
     CDS             complement(2332..2835)
                     /locus_tag="IV228_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8717113.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3245..3934)
                     /gene="rplA"
                     /locus_tag="IV228_001182"
     CDS             complement(3245..3934)
                     /gene="rplA"
                     /locus_tag="IV228_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8717114.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4038..4460)
                     /gene="rplK"
                     /locus_tag="IV228_001183"
     CDS             complement(4038..4460)
                     /gene="rplK"
                     /locus_tag="IV228_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8717115.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4803..5471
                     /gene="sdaAB"
                     /locus_tag="IV228_001184"
     CDS             4803..5471
                     /gene="sdaAB"
                     /locus_tag="IV228_001184"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8717116.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5484..6356
                     /gene="sdaAA"
                     /locus_tag="IV228_001185"
     CDS             5484..6356
                     /gene="sdaAA"
                     /locus_tag="IV228_001185"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8717117.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6447..7466)
                     /locus_tag="IV228_001186"
     CDS             complement(6447..7466)
                     /locus_tag="IV228_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8717118.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7639..8526)
                     /locus_tag="IV228_001187"
     CDS             complement(7639..8526)
                     /locus_tag="IV228_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8717119.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8603..9148)
                     /gene="nusG"
                     /locus_tag="IV228_001188"
     CDS             complement(8603..9148)
                     /gene="nusG"
                     /locus_tag="IV228_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8717120.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9249..9419)
                     /gene="secE"
                     /locus_tag="IV228_001189"
     CDS             complement(9249..9419)
                     /gene="secE"
                     /locus_tag="IV228_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8717121.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9443..9595)
                     /gene="rpmG"
                     /locus_tag="IV228_001190"
     CDS             complement(9443..9595)
                     /gene="rpmG"
                     /locus_tag="IV228_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8717122.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9757..10401
                     /locus_tag="IV228_001191"
     CDS             9757..10401
                     /locus_tag="IV228_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8717123.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10457..10960)
                     /locus_tag="IV228_001192"
     CDS             complement(10457..10960)
                     /locus_tag="IV228_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8717124.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10973..11019)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11557..12522
                     /locus_tag="IV228_001193"
     CDS             11557..12522
                     /locus_tag="IV228_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8717125.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12621..13793)
                     /locus_tag="IV228_001194"
     CDS             complement(12621..13793)
                     /locus_tag="IV228_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717126.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13780..14925)
                     /locus_tag="IV228_001195"
     CDS             complement(13780..14925)
                     /locus_tag="IV228_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717127.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14939..15871)
                     /locus_tag="IV228_001196"
     CDS             complement(14939..15871)
                     /locus_tag="IV228_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717128.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15988..17067
                     /locus_tag="IV228_001197"
     CDS             15988..17067
                     /locus_tag="IV228_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8717129.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAIQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17064..17682
                     /locus_tag="IV228_001198"
                     /pseudo
     CDS             17064..17682
                     /locus_tag="IV228_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17792..18019)
                     /locus_tag="IV228_001199"
     CDS             complement(17792..18019)
                     /locus_tag="IV228_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8717130.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18122..20710)
                     /locus_tag="IV228_001200"
     CDS             complement(18122..20710)
                     /locus_tag="IV228_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8717131.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20730..21389)
                     /locus_tag="IV228_001201"
     CDS             complement(20730..21389)
                     /locus_tag="IV228_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8717132.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21438..21941)
                     /gene="trmL"
                     /locus_tag="IV228_001202"
     CDS             complement(21438..21941)
                     /gene="trmL"
                     /locus_tag="IV228_001202"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8717133.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22162..23028)
                     /locus_tag="IV228_001203"
     CDS             complement(22162..23028)
                     /locus_tag="IV228_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8717134.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23137..23778
                     /locus_tag="IV228_001204"
     CDS             23137..23778
                     /locus_tag="IV228_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8717135.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23840..25201)
                     /gene="mgtE"
                     /locus_tag="IV228_001205"
     CDS             complement(23840..25201)
                     /gene="mgtE"
                     /locus_tag="IV228_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8717136.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25219..26118)
                     /locus_tag="IV228_001206"
     CDS             complement(25219..26118)
                     /locus_tag="IV228_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8717137.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26120..26917)
                     /locus_tag="IV228_001207"
     CDS             complement(26120..26917)
                     /locus_tag="IV228_001207"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8717138.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26895..27599)
                     /locus_tag="IV228_001208"
     CDS             complement(26895..27599)
                     /locus_tag="IV228_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8717139.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27730..28305
                     /locus_tag="IV228_001209"
     CDS             27730..28305
                     /locus_tag="IV228_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8717140.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28423..29100
                     /locus_tag="IV228_001210"
     CDS             28423..29100
                     /locus_tag="IV228_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8717141.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6167 c         5231 g         9139 t
ORIGIN      
        1 gtaaatacag tattatttcg gttgcttgct gcttcaccta gcccttgtac tcgtgtttga
       61 ttggcgactt gaccaaattg attcccgacg tctaataaaa taatagctaa aagcaaaatg
      121 gccaaatggc ttccgccaac caataaagca ataaagctta ttgtttgcat aacaagtccc
      181 atatacacga tttttctttc agaataagaa tctgccagtc gtcccacgat aggtgcagca
      241 actgctccag acaaaccaaa aatcgctaat atccctgctt cgaatgttcc ccaattgtag
      301 gcgggactgc tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt tccaaacatg
      361 aaaaatccac tgactgccgc ttctcttaat agacgttgag aagcaactaa atgcggcaaa
      421 cttttaagtg aatcaaggta tgaaacagct gtactacttt gctggacaga acgcggcatt
      481 ttccagtaaa gcagaagtgt aagaattccg actgcacata aagcaaataa atagatcatc
      541 cgccaagaaa aaagactggc aacaatacca gataccgtcc gagaaagcaa aatccccacg
      601 agtaaaccgc taagcaatgt acccatgacc ttccctcttg cttttggtcc cgctagtacc
      661 gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta atcctattaa aaaagaagcg
      721 catgcaaata acgtaaacga aggcgcaaaa aaagcggcca acagagagac ggcagataac
      781 gctaccattc gaatgatcaa ttttggacga tccaccacgt cccctaaagg aactaaaaac
      841 agtaacccta acgcatagcc taattgggtc aacatcgtca atatcccgac tgcacttgtg
      901 ctaacagata aactagctgc tacttttgtt ccaatcggct ggatgtaata catattggca
      961 acaacaacgc cacaagtgat tgataatagt aaaataagcg aagttgttaa aggtgttttt
     1021 tcattactct tcattgattt cccccatttc ctatcttagt ttacaactta tggaaaagat
     1081 tggaaataga aaaatacaac tattcgccac acttattatt tgtgcttttg ttttccttgt
     1141 tttttcttgc gatctttata aatgaagaca actaacccta ctagcacagt aacacttgca
     1201 accaacgtga ctgcagtttt gttatatccg tgaaacagag cttctccacc acacgcatac
     1261 aacacagacg aagggatgac tccaatcacg atcgacaata tgatttgctg tagaggcaat
     1321 ttcatcgcat ctgcagcaaa attgaccata gaagatggaa taaccggtat catatagcct
     1381 aacattacac ctatttttgg atgtttcatt tgtcggattg cttgcaccca atgattcgtt
     1441 tcggttttct tatctaaaaa ttttaagcga tgcataagga aaattgcagt gatgttacca
     1501 agagcattac ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct caaaccagca
     1561 atgactccaa ctactgaagt aggaatacct ggaacagcac acatcaccgc tgtcaatacg
     1621 atcaagagta tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag ctctgcctta
     1681 ctcgctctcg gatttaaaaa taattggatg tctgtctgat attctaaata caaccgatag
     1741 cccactacca gtatcagaat gattcccaac gagatcaaca tcaatcggat tattttcttt
     1801 ttcatatttc ctcctgaaaa aaatctatat tcattaaata atagtttaac ataaaaacaa
     1861 aaaagcaccg aaatccaaca taggatctca gtgcttttaa atagtaaaaa ttatttaaca
     1921 gttacagatg cgccaacttc ttcaagttta gctttcaatt cttcagcttc ttctttagat
     1981 acgccttctt taactggtgc aggagcgcca tcaactacag ctttagcttc tttcaagcct
     2041 aaaccagttg cttcacgaac tgctttgata actttaactt tttggtctcc agctgcagtt
     2101 aattctacag tgaattctgt ttgttcttca gcagcagcag cgccaccagc agcagctaca
     2161 gctacaggag cagcagcaga tacgccaaat tcttcttcaa tagctttaac taggtcgtta
     2221 agttctagga tagttgcttc ttttaactca gcaacgatgt tttcaatgtt caatgccatt
     2281 ataatttcct ccgtttaatt gttctttttt atttaatgct ttatgcggca attaagctgc
     2341 atctccatct tctttgtctg ccactgcttt gacagcgtaa gccacgttgc ggactggcgc
     2401 ttgtagtaca gatagcaaca tagatagaag tccttcgcga tttggcaatt ttgccaatgc
     2461 tgtgatttct tccaatgaag agactttacc ttcgatgata ccgcctttga tttctaatgc
     2521 ttttgcttct ttagcaaatt cgtccatgat tttcgctggt gcgactacat cttcgttact
     2581 gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc cagctttttt
     2641 agctgcacgt gaaagaatag aatttttgat aactttcatt tctacgttag catcacgaag
     2701 ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa cgactactac
     2761 tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg attgctgctt tttttgcgat
     2821 aattgcttca ctcatttttg agtttcacct cctgaatttt gatggaagag ttttttgcac
     2881 tgaaaaggca agtcactttt atagttactt tgctcaacca gtattattca tcacctattc
     2941 acttcgttca tagtgattct actcgctttg ctcgaaagga aactcctttc gctttgctca
     3001 accagtattg ttcatcatct cttcacttcg ttcatagtga ttcacaataa aaaactctat
     3061 gccaccgcag acatagaggg actattgaat atattcaata aacgtcctcg gtaggaaatt
     3121 aaggcttgat gccacctact gtcttcggta caagttaatt attaacctca actagcttag
     3181 catgctaaac tagttgagtc aagaattatt tttcagttgg atcaatcaac attctaatat
     3241 ttaattagaa agaagcttgg tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta
     3301 catttttcat gtattgacct ttagctgctg atggtttagc ttttagcaat acatcatgga
     3361 ttgctgcaaa gttttcaact aatttttcat cgtcaaatga aacttttccg attggtacat
     3421 ggatatttcc agctttgtcc acacggtaag ttactttacc agctttcact tcgtttactg
     3481 cttttgttac gtccattgta actgtaccag ttttagggtt tggcattaag ccttttggtc
     3541 ctaatactcg tcctagacga cctacagtag ccatcatatc aggtgttgca acaactacat
     3601 caaagtcaaa ccatccgttt tggatttttt gaaccatgtc gtcgtcacct acgaaatctg
     3661 caccagctgc ttcagcttct tttgctttgt ctcctttagc aaaaactaaa acagtttgtg
     3721 ttttaccagt tccgtttggt aatacaacag caccacggat ttgttggtct gcttttttag
     3781 ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc aaattttgca atgtttgttt
     3841 cttttgctaa agctactgct tcggcaacag gataaacttt acttgaatca acttttttta
     3901 atgcatcttg catttttttg ctctttttag ccattttggt tcctccttga ttgtggttat
     3961 aacggttgta cctcccacgt gttctatatc tttcaatata gagccaactg agaagctgct
     4021 gtcttagggt gtatagatta ttctacagtg atccccatgc ttcgtgcagt tccttcaacc
     4081 atgcgcattg ctgcttcaac gttagctgcg tttaggtctt ccatttttaa ttcagcgatt
     4141 tctcttactt gatcgctaga aactttcgca actttatttt tgtttggttc gcctgaacct
     4201 ttttcgattt tagctgcttt tttcaataga acagcagctg gcggtgtttt tgtaacgaat
     4261 gtgaatgaac ggtcttcgta tacagaaata acaaccggaa tgatcaaacc tgcttgatca
     4321 gctgtacgag cgttgaattc ttttgtgaat cccatgatat tgatacccgc ttgacctagt
     4381 gcaggaccta ctggtggagc tggagttgct ttacctgcag gaatttgcaa tttaacgatt
     4441 ttttctactt tctttgccac gagacatacc tccttaagtc cgtgatgtgg ttaattggag
     4501 atgcatattt ctcctcccac gttttatctg atctgaaaaa atccagacaa ataatgtgca
     4561 aaacaatgca cactggtaca gtatagcaaa tttatttgtt ttttcaagat ttttttctgc
     4621 tatttttaca ttttgcttga ataaacgcta aatttattct aaaaaaacaa caatttttcg
     4681 ttctcatttc ttcttttatg ttacacttgc gctagcatgg ttcaacagat gaaagcaaac
     4741 aataacacgg ttttttagac ccttgtacaa agattcctac ataacaaaat gaggtggaga
     4801 aaatgaaaga tttaaggtat agaagtgtct ttgatattat tggacctgtg atgattggtc
     4861 caagtagctc tcacactgcc ggagctgcta gaatcgggaa aatcatacgt agtatatttg
     4921 gcgaacagcc tgattctgta gatatttatc tatatgaatc atttgcaaaa acctatcggg
     4981 ggcacggaac agatattgca ttagttggcg gcctgttagg gatggaaccc gatgatgaac
     5041 gtttagctga ttctttgaaa ttggcatatg aagccggtat ggaagtcttg tttgttccta
     5101 agagtgaaaa agccgaacat ccaaactctg taaagatgct tctaaaaaaa ggaacacgaa
     5161 agctttcagt cactggcatt tcgatcggcg gtggcaatat ccagatttct gaattgaatg
     5221 gattcaaaat ttctttaagc atgggtactc ctaccttgat cgttgtccac caagatgttc
     5281 caggtatgat tgctaaagtg actagtatac tttctgagac caatacgaat atcggcacta
     5341 tgacagtgac acgggaatca aaaggtgaaa atgccattat gataattgaa gtagatgatc
     5401 ctcaagtaga agaaacggta aaaagactca aacagctgcc aaatatcgac agcgtaaatt
     5461 atttcgatta aggagctaaa aaaatgtttt tatcgattga agaattagta gatcaagcaa
     5521 aagcttatcc aagtgtagca gaattgatga ttgcagttga aatggaaatg agtggccgtt
     5581 cccgtagtca catcatcgag atcatggaaa aaaacctctc agtaatggaa caatcaatca
     5641 acgagggaaa agaaggtgtt acctccgtca caggaattac cggtggtgat gcaaaaaaat
     5701 tagatgatta tttaaatgag gcagactttt taagtggaga aacaatctta aaagctgtac
     5761 gcaatgctgt agctgttaat gaagtcaatg caaaaatggg gttgatttgt gctacaccga
     5821 ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat cggcttgatt
     5881 tgacacatga acagcaattg gaattcttat ttaccgctgg cgctttcggg ttagtcatcg
     5941 caaacaacgc ttctatcagt ggtgcagaag ggggatgtca agcagaagtt ggttctgcaa
     6001 gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg gactcctcat caagcagcac
     6061 aagcagtagc cattacattg aaaaatatga tgggactgat ttgcgatccc gttgcaggct
     6121 tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct tttatctctg
     6181 ctgatatggc attagcgggt atcgaaagtg tcattccgcc ggatgaagtc gttgctgcaa
     6241 tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa ggtggcttag
     6301 ctgttacgcc aactgctaaa cgacttgcgg aagagatttt aaataagagg gcgtgataaa
     6361 gctccttaat ttcgagtatt aagagattag ttatcttcat aacaaaaaac gaagagagga
     6421 ctaattgccc ttctcttcgt tttttattaa tccatcaatt tcgtattttt tacttcatcg
     6481 atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag gtgttcaaaa
     6541 acagatgtta ctccttctgc cccacctaga ttcaacccat aaatcactgg tcggccaatc
     6601 gcaactaagt cagcaccact tgccagagct ttgaagacat gctctcctcg acggacacca
     6661 gagtcgaaaa tcactggtac acgtttgttg actacttcgg caatttttgg caatacttca
     6721 aaggacgcag gacctccatc taattgacgt ccaccatgat tggataccca gataccatct
     6781 gcccctgcag agatagcgat tactgcatct tctggcgatt gtattccttt tacgataact
     6841 ggaagatggg tgatttcttt gattgttttg atgtcatctg gtgtcaagcc ttgttttgca
     6901 gctgcataaa tttctgcgat tccttttcct tcgccattac cacttgcact ttgttcgcta
     6961 tacgcagcta agttaggcat tggtaatgga aattggaatt gattgatgac gtcttcttca
     7021 cggtaaccac cgagcgttga atctgccgtt aaaatgatag ctttagcccc tgcttctact
     7081 gctttgttca aaatgaactc attgaatctg tcgtctttac tcatatataa ttggaaaaac
     7141 tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg tcgtatttgc ataagtgctg
     7201 atggaaaaga ttgatccagc agcagctact cctttagctg tatcagcttc ccctttttca
     7261 tgtgccaatc cttgggccgc tgatggcgct tggataatcg gtgtatctaa ttcaattccg
     7321 aaaacagatg tatgcaaatc ggctgagtcg attccgcgta atatccgtgg catgattttc
     7381 tttgtattaa aagaagtcgt attttctttc atcgtccact catcttcaga accaccgcga
     7441 atatagccaa aagctcctgc ttccatgcgc tcttttacac gaccttctag ttaggcaatg
     7501 tttacaatat cgattggatg ttcttcatta cttgtatgat aagtcatagt agatccttct
     7561 ttcatcttct tgattacata taaatcatac taaacttaca aaaagtaagc aaacatttac
     7621 tctcagatac ttttatcgct attctttcca aataaatcga cttacaagct gatctacttc
     7681 ttggttttca tgaagcatac tatgagaggc attttcacct gtcactactt tttcctctat
     7741 ttcatttccg cgttgcttca acaacgcata gaccgctaat gcactgttta acggtactgt
     7801 tccatcactc aagtctgttt catctagttg accagcaatc aagaaaaatc gagtggttat
     7861 aggaatatta ccgatcattt gctggtaatc ttgatagcgg ttagaaacga cagcaggccc
     7921 ctttgctaac tcatctgtca gagactgcgc actatcatcg acaaaatcat taaaaggcgc
     7981 gccaattgct gttaatgtct gaacttgtgg caatgacgta tcctgtccat acgttcctaa
     8041 atagcgtagt gcactaactc ctcccatgga atgccccact atgttgactt tatctatgcc
     8101 gtagttttct ttcagataca aaagacaagt tttgatccat tctgcttgat tccactcgtt
     8161 atttttatta tcttcaaaca atacttggac catcggattg tctgcttttc cagacaaatc
     8221 tcctgttgct tgtatttgtc catctgttcc tactgttaaa accaattctt ttactgcttg
     8281 ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc cgctgtagcc
     8341 gtggaaaaaa agtgttggaa tagtggtttt ttctatcact ttactggatt ctgccgtttt
     8401 ttctttatca gaaacttcag tagtatttat cttcgtcgtc tggcaaccac ttaacgcaaa
     8461 caaactgata aggactaaag ctgctgcagt tgcacgtttg cataaatttt tgattggttc
     8521 tgtcatacac ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc caatcatatg
     8581 atcgacattt caagtctctt tattaatcga ttttatctac ttgatcgaaa tcaagttctg
     8641 tactggtttc acgtccaaac atatcgatgt tgactttcaa tttttctttt tctccgtcaa
     8701 cttctgttac ttgtccttct aatccagcaa atgcgccttc gatgattttc acgacttcgc
     8761 caacttctac ttctagatca ttttgtcgca ggctcatacc gatagaacgc aagatatgat
     8821 tgacttcttc tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa ccaacgaatc
     8881 ctgtcacacc aggtgtgttg cggacaacat accatgaagc gtctgtcatg atcatttcaa
     8941 caagtacgta tcctgggaat gttttatgaa cgatttcttt ttctttccca tttttgactt
     9001 cagtttcttt ttcttcagga acaacgacac ggaaaataaa gtcggccatt ttcatacttt
     9061 gcgcacgaga ttcgatattt gcttttactt tgttttcata tcctgaatat gtatgcaaga
     9121 cataccagtt tttttcaaaa gattccattg aaggctcctc atttcattgt aaaataaaaa
     9181 aacctcagta cacccgaagt ttttctttcg cttcattata gcactaaaag tacgttgtgg
     9241 ctagcgagtt attttaaaat ccagccaaat aagctttgga tggctgtatc cattacgtaa
     9301 aacatcaatc cgaaaataat agaagtttcg atgactacta acgtatcttt acggagttgt
     9361 tttctgcttg gccaagttac ttgtttgatt tcatctttta cgctttttag aaatttcatg
     9421 agacaacctc ccttgctact gtttatttcg tttctttatg taatgtatat tgattacaat
     9481 atttacaaaa cttattgatt tccaaacgtt cgccgctttt tccttcactg actgattttg
     9541 tgtagttgcg agacccacaa acggaacaag cgagtgctgc ttttttcttt gccatttact
     9601 tctgccccct tgctgagaat cagacttata cttcataaca gtatcacgta actttttact
     9661 tgtcaatttg tgaaataaac tatctattgt tttggataaa attatgatat gattaatttg
     9721 aaattaagct aaaatgagac aattggaggt atagcgatgc tattaaaaac acttgtatat
     9781 aagaaaaaag atctgacaac cgttacagag actagtacat tagaagaagc attaaaaatt
     9841 ttagaaactt ccggataccg ttgtgtgccg atcttagatt cttcaggtaa tattttccgt
     9901 ggaaacattt acaaaatgca tatctatcgt cataaagcaa atggcggtga tatgacgctt
     9961 cctgtcactc atcttttgaa aaacgctaca aagttcattt acctggatac ttctttcttc
    10021 aaagtattct ttacgatcaa agagcttcct tatattgctg tccttgacag tgaaaatcat
    10081 ttttatggta ttttgacaca tagtacgttg cttaatatgc tctctcaatc ttggagcgta
    10141 gaacaaggaa gctatgtact gacaattgct tccacaggaa agcaaggaga tttggctgct
    10201 attgcaaaaa tcatttcaaa acacagcagc attgccagct gtattactct tgatatcgaa
    10261 aaagacgaat acattcgacg tacattgatc acgctaccgg ctaacactga agcgaaagta
    10321 tgtgaaacga tcgtcaacca cttagagaaa aagaattata aagtggtaga aattgaaaat
    10381 ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa aacagctaat ctgctttctt
    10441 ttttcgcttt ttataattag acacgctttt caaaatcaag cgcgcgatct aaataataca
    10501 tcaatggtgc tgaaacagcc atgacgataa actcacttaa tgcaagcgtg ccataggttg
    10561 tccaaaatgc ttggccacct gttggaacta acatataagc aatcagtccc atgctgatgg
    10621 taaagaagat cgtgttcaat actaaacgca tctttacatt tttcactttg ttttgcaata
    10681 aagctgtcag gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca tccatgatac
    10741 caaaaccaaa gaaggcatta tagacgatga ccccgcctaa aactccccaa agcaatttac
    10801 ggttaaagac aactaaatga ttcaaacttt ctgaaatacg aaactggatc ggccctgagg
    10861 atacaggcgc aactaaaata gttaacgcta gataaagagc catgatgatg ccatttacaa
    10921 cgattacgga taaccgttgt ctgcttttcg ttttttgcat tttctctcct cttttctcct
    10981 agttttttta acgcgggatg gttgatgaac cgcggttatg tgaaacaaac aataattata
    11041 acagactttt catttatttc aatccgtctt atctgctttt aaaaaagcaa aagcttaaac
    11101 aatgtaaacg tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa ggggtcataa
    11161 gaattcccag taaagcagac gaagtaggat ctgcaagaac agaattgttg tgaaacttaa
    11221 ataaataaaa aaggatgtgg gaatttgagc aacctcttac tagagaacgg tctagcaagc
    11281 agctgaagat ctaagtggac atgccgaacc ttggaacttc tccttacgta agtaatggat
    11341 catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca ttttcctaga
    11401 cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa aacaagtttc
    11461 tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa caggagtatt
    11521 aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta tcatatattg
    11581 atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat tttaccggta
    11641 ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg acacgatgct
    11701 gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga agaacagaaa
    11761 acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt acatcaggta
    11821 cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc agcaggttct
    11881 ggaaatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg acttcagctg
    11941 attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga aaaaataagt
    12001 gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc tatcgtccat
    12061 gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg atcattttcg
    12121 tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat tctggtagat
    12181 gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac gaagcatcct
    12241 ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc agtcattgat
    12301 tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct tttacttata
    12361 cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag attacgtatc
    12421 gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa acgacctttt
    12481 gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag gttttgtcag
    12541 aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac ttttgtcaca
    12601 gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg atagtttctt
    12661 tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac taagacacaa
    12721 ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc actgatcaag
    12781 ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct aaccatttct
    12841 ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt gataaaaata
    12901 tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca agccgaaaag
    12961 ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac cgttatatcc
    13021 cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa caacagcagc
    13081 gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt aatgggcgca
    13141 ctctggtttc gtttactgat ctctttgcga ttaaacgcta gattgatgac gctgaaaccg
    13201 ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc cgtcaacagc
    13261 tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac cgagagtgcc
    13321 atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa cagaatcagt
    13381 gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc atctgggcga
    13441 ctctgtatct tgatcgtcgc catctttcct acttcgactt gttcggtaaa gtctgcaggc
    13501 agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc tttttgtgag
    13561 gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag gccatcgact
    13621 acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga ggtattttca
    13681 acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac acctatcagt
    13741 aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt cataacgcac
    13801 ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga tcaaccatag
    13861 aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca aactttcgct
    13921 gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa tccaatactt
    13981 cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca ctgaaatcaa
    14041 gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca atgtacctaa
    14101 caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc aacgttcacc
    14161 aaagtctact tgatagataa aacgaaagac agctaacata atataaatct ctgtcaaaac
    14221 aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc taggaatcaa
    14281 ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat tgcggacccc
    14341 ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa aacttttgaa
    14401 actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa aaacagtcat
    14461 gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta taccaatacg
    14521 ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg cttgacccac
    14581 atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat ctgcatttag
    14641 cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt tatcattgac
    14701 ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat ccgctttgat
    14761 tacaattcca gtattaatcg tttcaagatt gccaccttga tcaatctcgc caaaggccgc
    14821 cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca tggatttatt
    14881 tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt cccctcctct
    14941 agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac gcttctttgg
    15001 ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc ggcaacaata
    15061 ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg attccatcag
    15121 aagctattgt tcgctgaatc gctgacaaca cattttctgg gagtaatggg atgtcgatcg
    15181 tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca
    15241 cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat
    15301 gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt
    15361 tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct
    15421 tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg
    15481 gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt
    15541 tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac
    15601 ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat
    15661 aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca
    15721 aagaaagcat acagttgatg gcagtcgatt tcccactgcc gtttggacct aacagaccta
    15781 agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc
    15841 gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt
    15901 gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg
    15961 acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc
    16021 tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatacaagtt
    16081 atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca
    16141 aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg
    16201 tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt
    16261 ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt
    16321 gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat
    16381 cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg
    16441 gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa
    16501 gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg
    16561 attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta
    16621 gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac
    16681 ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct
    16741 tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc
    16801 ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc
    16861 gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc
    16921 accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt
    16981 caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc
    17041 attttaccaa gggagggaca ccaatgataa cagtagtcat tattgatgat gatccatttg
    17101 tgacgaaatc gttacaaacc attttagaaa gcacgaaaga gatccgtgtg ttaggcattg
    17161 gtcattgtgc caaggatgcg cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
    17221 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
    17281 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
    17341 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
    17401 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
    17461 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
    17521 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
    17581 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
    17641 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
    17701 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
    17761 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
    17821 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
    17881 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
    17941 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
    18001 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
    18061 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
    18121 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
    18181 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
    18241 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
    18301 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
    18361 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
    18421 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
    18481 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
    18541 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
    18601 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
    18661 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
    18721 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
    18781 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
    18841 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
    18901 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
    18961 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
    19021 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
    19081 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
    19141 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
    19201 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
    19261 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
    19321 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
    19381 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
    19441 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
    19501 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
    19561 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
    19621 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
    19681 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
    19741 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
    19801 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
    19861 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
    19921 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
    19981 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
    20041 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
    20101 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
    20161 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
    20221 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
    20281 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
    20341 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
    20401 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
    20461 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
    20521 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
    20581 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
    20641 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
    20701 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
    20761 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
    20821 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
    20881 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
    20941 aaaagtgggc catctttatg tcgggcaacc gcatctgcta caacagtttc gatacgtgtg
    21001 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
    21061 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
    21121 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
    21181 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
    21241 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
    21301 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
    21361 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
    21421 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
    21481 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
    21541 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
    21601 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
    21661 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
    21721 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
    21781 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
    21841 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
    21901 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
    21961 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
    22021 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
    22081 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
    22141 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
    22201 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
    22261 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
    22321 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
    22381 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
    22441 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
    22501 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
    22561 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
    22621 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
    22681 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
    22741 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
    22801 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
    22861 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
    22921 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
    22981 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
    23041 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
    23101 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
    23161 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
    23221 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
    23281 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
    23341 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
    23401 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
    23461 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
    23521 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
    23581 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
    23641 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
    23701 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
    23761 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
    23821 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
    23881 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
    23941 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
    24001 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
    24061 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
    24121 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
    24181 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    24241 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    24301 cggtcgacat acctaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    24361 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    24421 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    24481 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    24541 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    24601 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    24661 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    24721 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    24781 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    24841 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    24901 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    24961 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    25021 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    25081 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    25141 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    25201 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    25261 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    25321 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    25381 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    25441 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    25501 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    25561 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    25621 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    25681 atgctttgca aaaagcatca agccagatgt atcccgatct agtcgcgtca ccacatggat
    25741 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    25801 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    25861 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    25921 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    25981 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    26041 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    26101 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    26161 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    26221 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    26281 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    26341 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    26401 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    26461 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    26521 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    26581 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    26641 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    26701 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    26761 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    26821 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    26881 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    26941 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    27001 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    27061 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    27121 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    27181 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    27241 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    27301 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    27361 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    27421 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    27481 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    27541 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    27601 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    27661 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    27721 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    27781 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    27841 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    27901 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    27961 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    28021 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    28081 caaaccggaa agcttacaac aaaacgagct gaattcccta ttgaagaagg acttttagct
    28141 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    28201 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    28261 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    28321 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    28381 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    28441 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    28501 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    28561 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    28621 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    28681 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    28741 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    28801 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    28861 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    28921 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    28981 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    29041 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    29101 aa
//
LOCUS       DACDHL010000026        28776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDHL010000026 DACDHL010000000
VERSION     DACDHL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.026"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..1259)
                     /locus_tag="IV228_001211"
     CDS             complement(27..1259)
                     /locus_tag="IV228_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8717142.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="IV228_001212"
     CDS             1514..2083
                     /locus_tag="IV228_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8717143.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="IV228_001213"
     CDS             complement(2261..2701)
                     /locus_tag="IV228_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8717144.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="IV228_001214"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="IV228_001214"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8717145.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="IV228_001215"
     CDS             3655..4578
                     /locus_tag="IV228_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8717146.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="IV228_001216"
     CDS             complement(4803..5042)
                     /locus_tag="IV228_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8717147.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="IV228_001217"
     CDS             complement(5047..5466)
                     /locus_tag="IV228_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8717148.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /locus_tag="IV228_001218"
     CDS             complement(5693..6931)
                     /locus_tag="IV228_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8717149.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="IV228_001219"
     CDS             complement(7038..7673)
                     /locus_tag="IV228_001219"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8717150.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7787..7879)
                     /locus_tag="IV228_001220"
                     /pseudo
     CDS             complement(7787..7879)
                     /locus_tag="IV228_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8015..8650)
                     /locus_tag="IV228_001221"
     CDS             complement(8015..8650)
                     /locus_tag="IV228_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8717151.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="IV228_001222"
     CDS             9162..10304
                     /locus_tag="IV228_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8717152.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="IV228_001223"
     CDS             10297..10503
                     /locus_tag="IV228_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8717153.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="IV228_001224"
     CDS             10582..11775
                     /locus_tag="IV228_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8717154.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(11782..11940)
                     /locus_tag="IV228_001225"
                     /pseudo
     CDS             complement(11782..11940)
                     /locus_tag="IV228_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            11980..13119
                     /locus_tag="IV228_001226"
     CDS             11980..13119
                     /locus_tag="IV228_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8717155.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="IV228_001227"
     CDS             13112..13912
                     /locus_tag="IV228_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8717156.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="IV228_001228"
     CDS             13912..14739
                     /locus_tag="IV228_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8717157.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14717..15451
                     /locus_tag="IV228_001229"
     CDS             14717..15451
                     /locus_tag="IV228_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8717158.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="IV228_001230"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="IV228_001230"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8717159.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="IV228_001231"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="IV228_001231"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8717160.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="IV228_001232"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="IV228_001232"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8717161.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="IV228_001233"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="IV228_001233"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8717162.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="IV228_001234"
     CDS             19036..21069
                     /locus_tag="IV228_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717163.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="IV228_001235"
     CDS             21113..22393
                     /locus_tag="IV228_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8717164.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="IV228_001236"
     CDS             22603..23409
                     /locus_tag="IV228_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717165.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="IV228_001237"
     CDS             23421..24641
                     /locus_tag="IV228_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717166.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="IV228_001238"
     CDS             24631..26217
                     /locus_tag="IV228_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8717167.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="IV228_001239"
     CDS             26256..28394
                     /locus_tag="IV228_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8717168.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         9213 a         5037 c         5777 g         8749 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca
    28561 aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg
    28621 tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca
    28681 gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc
    28741 gatattttat tttaatatag gctattttaa aataaa
//
LOCUS       DACDHL010000027        28243 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDHL010000027 DACDHL010000000
VERSION     DACDHL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.027"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(97..837)
                     /locus_tag="IV228_001240"
     CDS             complement(97..837)
                     /locus_tag="IV228_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717169.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(967..1914)
                     /locus_tag="IV228_001241"
     CDS             complement(967..1914)
                     /locus_tag="IV228_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8717170.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2043..2807)
                     /locus_tag="IV228_001242"
     CDS             complement(2043..2807)
                     /locus_tag="IV228_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8717171.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2811..3200)
                     /locus_tag="IV228_001243"
     CDS             complement(2811..3200)
                     /locus_tag="IV228_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717172.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3335..3877
                     /locus_tag="IV228_001244"
     CDS             3335..3877
                     /locus_tag="IV228_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8717173.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4025..4657
                     /locus_tag="IV228_001245"
     CDS             4025..4657
                     /locus_tag="IV228_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717174.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4791..4916)
                     /locus_tag="IV228_001246"
     CDS             complement(4791..4916)
                     /locus_tag="IV228_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8717175.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5050..5943)
                     /locus_tag="IV228_001247"
     CDS             complement(5050..5943)
                     /locus_tag="IV228_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8717176.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6125..7051)
                     /locus_tag="IV228_001248"
     CDS             complement(6125..7051)
                     /locus_tag="IV228_001248"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8717177.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7136..7897)
                     /gene="pflA"
                     /locus_tag="IV228_001249"
     CDS             complement(7136..7897)
                     /gene="pflA"
                     /locus_tag="IV228_001249"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8717178.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8141..10372)
                     /gene="pflB"
                     /locus_tag="IV228_001250"
     CDS             complement(8141..10372)
                     /gene="pflB"
                     /locus_tag="IV228_001250"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8717179.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10649..13099)
                     /gene="parC"
                     /locus_tag="IV228_001251"
     CDS             complement(10649..13099)
                     /gene="parC"
                     /locus_tag="IV228_001251"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8717180.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13111..15156)
                     /gene="parE"
                     /locus_tag="IV228_001252"
     CDS             complement(13111..15156)
                     /gene="parE"
                     /locus_tag="IV228_001252"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8717181.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15322..15756)
                     /locus_tag="IV228_001253"
     CDS             complement(15322..15756)
                     /locus_tag="IV228_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8717182.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15883..16521
                     /gene="plsY"
                     /locus_tag="IV228_001254"
     CDS             15883..16521
                     /gene="plsY"
                     /locus_tag="IV228_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8717183.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16653..17528)
                     /locus_tag="IV228_001255"
     CDS             complement(16653..17528)
                     /locus_tag="IV228_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8717184.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17610..18401)
                     /gene="codY"
                     /locus_tag="IV228_001256"
     CDS             complement(17610..18401)
                     /gene="codY"
                     /locus_tag="IV228_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8717185.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18419..19819)
                     /gene="hslU"
                     /locus_tag="IV228_001257"
     CDS             complement(18419..19819)
                     /gene="hslU"
                     /locus_tag="IV228_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8717186.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19833..20381)
                     /gene="hslV"
                     /locus_tag="IV228_001258"
     CDS             complement(19833..20381)
                     /gene="hslV"
                     /locus_tag="IV228_001258"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8717187.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20418..21323)
                     /gene="xerC"
                     /locus_tag="IV228_001259"
     CDS             complement(20418..21323)
                     /gene="xerC"
                     /locus_tag="IV228_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8717188.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21521..22897)
                     /gene="trmFO"
                     /locus_tag="IV228_001260"
     CDS             complement(21521..22897)
                     /gene="trmFO"
                     /locus_tag="IV228_001260"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8717189.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22866..24944)
                     /gene="topA"
                     /locus_tag="IV228_001261"
     CDS             complement(22866..24944)
                     /gene="topA"
                     /locus_tag="IV228_001261"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8717190.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25066..25923)
                     /gene="dprA"
                     /locus_tag="IV228_001262"
     CDS             complement(25066..25923)
                     /gene="dprA"
                     /locus_tag="IV228_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8717191.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(25979..26746)
                     /locus_tag="IV228_001263"
     CDS             complement(25979..26746)
                     /locus_tag="IV228_001263"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8717192.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26739..27599)
                     /gene="ylqF"
                     /locus_tag="IV228_001264"
     CDS             complement(26739..27599)
                     /gene="ylqF"
                     /locus_tag="IV228_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8717193.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27859..>28243
                     /locus_tag="IV228_001265"
     CDS             27859..>28243
                     /locus_tag="IV228_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8717194.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPV"
BASE COUNT         7542 a         5991 c         4854 g         9856 t
ORIGIN      
        1 tgaggatacg atataaactt tcggatagag tataaaataa ggaaacgccg cagcgtttcc
       61 tttccaactg tagccgaata taaatttgta tatcacttat ttaaaatagc tattttctcc
      121 caaagagttt ccttgatcat cgatcaatcc ttgtaatgag cgttctaaga taccatggct
      181 ttttttcgat tttttttctt ctgactgctt tgtttcctct aacgtttttg ttcgattgaa
      241 tgttcgtaca gacggctcac catttctttt ttcttgatac ggcgtcggtt ctgtatcgaa
      301 caacagataa gagtcatccg tttttttcat cgattcgatt aaatgatcct cagaaatttt
      361 gctttctcta gggtctggga taatcgatgc gggtacatat ttaggaacaa aataagaacg
      421 ctcccgcacc ttcgtgtcaa cagatgcgtt tcgtgtagta tgcttggact ctgtccttgt
      481 ttcactttgt ctgacagaac gagaggaatc accaaaaagt ttttttcgtc ctgtcgatgt
      541 gatttctgat tggtaatggc cggtataatc cggtaatttt cctttatgct tttccaaatt
      601 ttccttacgc ggagctgact tcaatccatt tgttgcacga cgcttgcgac ggattttctc
      661 ggtgttcgtg ttcgtgaaat gaaattcttt catctgttca aacggggact gattctgttc
      721 agtcaaaaga tcttcttgtc cgtcaaataa tactcgcttc ttgtcgttgg ttaattttac
      781 gcctaactca tcatcgtacg caggaaaacg gaaatgtttt cgtctgattt ctttcattca
      841 aaccatcctt ctcaaaaatg gaacttttct caataatagc acagattcat tgttttcagt
      901 agcaatgcgc tgattatttt aagaaaatag cgatgaattt cataaatata tatatagtaa
      961 taagacttaa ttcgttgtct ctctgataac tagttcagga aaaaaacgac gataaccttg
     1021 actcgcccct ttttcttcta ttttttttaa caacatttct ccacatactt cacccatacg
     1081 tataatatct tgcttgatcg ttgtcaattt aggtgaactg atttggtcta aaaatacccc
     1141 atcaaatcca atcacaccaa attcttcggg caagtgtcct ccagcattta taatccctct
     1201 tactgctcct agtgctagcc gatctgaact acatacaaaa caagttttat ctgtaaaatc
     1261 tgcccagttt tctgtcatat atctttctac ttctgtcgaa tgatttggaa accgcaagat
     1321 tttactagga agatcaactt ccttcatcac agataaataa ccagtttccc ttgaaatttc
     1381 aaaaggctcg tccacttcga taccgatata aataatcgtt tgataatctc tttctaaggc
     1441 atattgcgtt gcttttgctg taccatattc attatcgctg tctacataat catatccttc
     1501 agaattttcc ccaaacaaga caactggttt ttccaaacgg tcgatccaat cgaagtcttt
     1561 ttttctgaca cctgtgatga tgtatccatc acaagaaccg acgtcgaagg catcatttgt
     1621 aactaattgg agagaataat gcgctttacc gatccgttta gcgattccca tcagcaaatt
     1681 catgtaatag ggctcagttg tgtcgatttc ttccagtata aataatttga tgatctgcga
     1741 ccgattcgtg acaagtgctt ttgcagcaat atttggacgg taatccagtt cagccattgc
     1801 tgagaaaact aactctttca attcgtcagt cacctgttcc ggatgattga tgactcgaga
     1861 gaccgtcatt tttgaaacat tggcacgttt tgcaacatct gctaaagtgg gcatgctctc
     1921 ttcctttcta acatgttgtc attcttacta tcaattatac caaattttcc taacagagtt
     1981 tattatagcc caaaataaaa aacggaaaat cctctatcgt catgagtgat tttccgtttc
     2041 aattatttat acttttttat caaatttttt gctaaaaaag tggaagcttc ttccattagt
     2101 ggtatttgta ccatatgtcg ctgtgttttt gaggaacgaa aaacaatttc ttttccgtaa
     2161 gacttatgcc gatttttttc tacaaatcgt tccgtttgta tataagggat tttttcgtca
     2221 gcttgtccat gccaaaagaa cagaggacgt ccagctaact tttccggctg caatgacaaa
     2281 tcatagctct taacccatga aatcaaatca aaatagtctt ttggtacgac ataattatat
     2341 tccgatgcgc gccttacgat ttcttttcca tattccaaag gtgcagggga acccatcaaa
     2401 cacacgcccg ctactatatg aggatgttta gccaacaagg cgcatgttgt cattcccccc
     2461 atcgaaaccc cgccaactgc aagctgatca tgtagaagac ctcttttttc taaatgcttg
     2521 ataagtgtgt caaattcaaa cagatttccg tagatactat cccaaaatgt ataggatgga
     2581 atagaagaaa ccggctgttt gcgctcccca tgattcatcg catctggcag gatcacacga
     2641 atgccctgct gcgcaatttt tcttccttgc gtcaaaacta actccttact tgtctgccat
     2701 ccgtgataat agatgacaac gggaatacgt tcctctctta attctttcag aacaacttct
     2761 aacactggaa tatgatggac agaacgtttc aatataattg gattcaattg ttactcacct
     2821 attttcatta aatttcctga tcgccatagc aaccatgctc caacagttgt ctggaacagg
     2881 ttcagtgtac caaataatat aaatgcagga ctgaatggct cgggaaataa gcacagagaa
     2941 caaagtccca ccactcctaa tgctagcgaa aggagtggat aaatagaaaa attcaaaaaa
     3001 ctggcaacgc taccgaaaat attaccaata aacagtatca aaaagaaaaa aacgcttaaa
     3061 attaaaccat ctgtcagcga catctgaaaa acaatgttct ctacctcaat tggctgttga
     3121 atcgaataga cacctaagta tatactgaga aggagactta gcacgtggct ggcacaaaac
     3181 acacgattga ccgttttcat tttaatcacc taacctatct ttataattct attataatca
     3241 ttttatcatt tttttgtatg tataaagttt caatttttct tcacttacga ttttttttga
     3301 tattgttgtt atataaatga aaggagtttt acaaatgatt gatccaaaaa tgttggggat
     3361 gatcttcctc attaattttg cttatatcac tctgaacacg ttacgtttca tgttgacaat
     3421 gaaaggctat cgactgattg ctccacttgt cagtatggtc gagatcacga tttatatcgt
     3481 cggcttatcc cttgttttag atcgtttaga taatccactg aatttatttg tctatgcgtt
     3541 aggttacgca gtaggtatca gtgtcggaat tttgattgaa gacaaattgg cattaggata
     3601 catcatggtc acaacgattt tgccagcaaa tagcgcagaa gataaaagtt tgccgggtat
     3661 actgagagaa cacggttatg gtgtgacaca gacttctgca atggggctgg aaggtgagcg
     3721 gctagttctc gaaatacttt ctcctagaaa aagcgaacgt gaactgtatc gcctgatcaa
     3781 agaaatcgaa gagcgtgctt ttatcatttc ttatgagcca aaatatattt caggtggttt
     3841 ctggactaaa aaagtgagaa aacgacaagg aaaataactc acaaacttaa aaactgtcta
     3901 atatctaaat taaattttaa atgttagaca gtttttattt attattttga ttaaaaaaca
     3961 tcgacatata ctcatcaatt tgttattata ctttatatac ttatcaattt aatttggaga
     4021 tattatggaa attcaacgta gaaccactaa ttttttacat ttaggctctt tttttgcaac
     4081 tttgacgatc ttattttttc tatgttattc ataccttaag acctcacatg cgccttctag
     4141 tgcattaggc tggttgcttt tggatcattc tgatatcaca gaagccgaag aaaatccgtt
     4201 ttttttgatt ttgtcttctc tttgcggctt actgttttct gtttatttcg gtgcctattt
     4261 ttttcatcga catcctggta cgttaaacga agcttcaagc gtattgattg catttaaaac
     4321 gttttctttt ttagcaattc ttttttattc gtttaatcac aatacgcttt tcttctttat
     4381 catgaatggt ttagtcgtga ttttatccat ttttacttat tttatttttt cactcatttt
     4441 tagccaattg aaatgtccgc tcttctttcg attagttcct gtcacctgtt ttatttacag
     4501 cttgttttcc ttttttgtcc ttgctttaat gccactgacc aacccttcga cgatccaagt
     4561 cgcaggaaat ctgttggata tgctcttcat cgtcagtcta ggtgtattta tgtggcaaag
     4621 aaaaaacaaa caaaataaaa agcattttga agcctgacta ttacgacttc ataaatgctt
     4681 ttttagtttt aaacacttca atctaaaaga ataacccaag attggataaa ttattaatga
     4741 ctcgccattc gtttcttagt gattctggat tatcaaatgg acttcctaat ctaatcagct
     4801 agttcttccc ataaaatgcc atacttgact gaatcataat attgattatt taaaaatcta
     4861 acttttctta tcactacttc ctttgtcatt ccacatttct caccattttt ttgcattcct
     4921 ttgtttccag accatgtagt gaatctaata ggtggtagat aggcaaaata ctcaaataat
     4981 tcttttaacc ataaaggaag agaaaggctc ctcctgcaag agtcagtata atttttgctt
     5041 actgctctct taggttttac tcactaaaac tgttccatat tctttgatat aagaaaatgc
     5101 atgcggtata aatttcttgc tcagctcaga aaagatttgt cgtttaagaa aggccatttc
     5161 ctccattgtc aatgaaggat ccaatgttgg taattcaatc tcaaaattgt ttttttcgac
     5221 aataggtaat tctaatgtga atgattcttc ttcctttata gtattcgtca caagtagaga
     5281 ctgatggaaa atactaggcc ggcgattttt atatttattc ttacgtatcc tgtcaacgat
     5341 aacttctatt tgatcaaaaa agtacgattc atttacgtct ccaataagtt cggctaattc
     5401 tttgaatgcc ttcgttacag gaagctgatt cttctgtaca aaaaagtaat tagccaaagt
     5461 aagcgggaag tgttctatag aggaaagtgt gataaaacgg tacttctcat ttcctgcagt
     5521 tgtcctcagc aattctgtct ctcggctgtt tcttactcca taaaaatacg cttctttccg
     5581 ttcatcttcg aaacaatacg cccaaataac ttctcccatc gctaattttt gttcatcaac
     5641 agagagtctc ttcaattgtt cagctatggt ttcagcagcc gaagtggctt gttgcaagta
     5701 atctttagga tcaaatattg gaaagttcaa atgatatctt cgttcttccc tcttgattaa
     5761 tcctgcatcg atcaataaat caagaaaatg ggaaaattta ttttgcgaaa attctttttt
     5821 caattctctt agaatcgttt cttgttctcg atgttcatat agataatcgg tcaatgaaga
     5881 aaaaagcgga tcatcggtca gtttttgata aaagttttcc ttattttttg aataatagtt
     5941 catatatgtt ctcttttcta tccgttttat tttacttaaa aggcttttac gtaaaaagga
     6001 ggaagaacaa gagtcataat tgactgttat tctcctccat ttcttcggcc tttagtatgt
     6061 tctatgtttc tttttatatc aatcgcttat ccattcattt tgaatgcttc atactaccat
     6121 ttgattagct aaatacagcc gtcaattgag gtacgacttg ttttttacgt gaaacgacac
     6181 ctttaagcaa agcacggttg ttggtcaaag ttgtttggaa agcttcttct actttttctt
     6241 ttcctttacc agcaacaagt aattctgaat cactgtctaa gatgtttgtg acaactaata
     6301 cgaacagatc gtagccattt gctttcattt catcttccat tgctgcttct agctctgctt
     6361 gtcgatccat cacttcatta aggtccactg tattgacttg agcgatacga acgttggaac
     6421 tgcccattgg gaaacttttt gcatccaaat caagaagaac cgcagctgat ttatcactta
     6481 gatttgttcc tgctttcaac atttcaagtc cataagtgtt tagatcgact tcagcgattg
     6541 ctgcaagttc ttttgcagct gcttcatctt caggcgtaca tgttggtgat ttgaacaaca
     6601 atgtatctga aatgatagct gagagcatga ttcctgcgat ttctttagga attgttactt
     6661 ggttttcttt atatagtttt aaaataatcg tacttgtaca tccaactggt tctgcacgat
     6721 agtacaatgg attagctgtt tcaaagtttg caatacgatg gtggtctact acagctaaaa
     6781 tatttacttc gctgatatct gaaatacttt gttggaattc attatgatcc acaagcatga
     6841 catctgcatt ttctgcttta gctgactcta ctactcgtgg tgcagatagc tgaaagtggt
     6901 ctaatgcgta ttgtgtttct tcattaggtg tacctagagc aactgcttct gtttctttac
     6961 ctaattcatt ttgcaagtgt gaaaacgcaa tggctgcccc gattgcatct gtatctggat
     7021 tttggtgacc aaaaactaaa atttttgaca tgtcattcgc tcctttgtga tttctcctat
     7081 ttaataatag caaaaaaacg gaaagaagca atacttatct tctttccgat gcgacctatc
     7141 gcgttaaata ttttgtatac tcttctgtat gaagcaatcg tttagcattt tcgacacgtt
     7201 cttttgtagg tggttcgatc ccttctaatg gatatttcaa accaagtgtt tcccacttgt
     7261 attttcccat cgtatgataa ggaagaatct ctactctgtc tacatttgac aaggtttgaa
     7321 taaagttgtc tagtcggatc aaatattcat cataatcact tcgctcagga actagtacat
     7381 ggcgaatcca aaccggtttg ttgatttttg acaaatattg agccatttct aaaatatttt
     7441 cattgtccca atgcgttagt tttttatgct cttcattatc gatgtgtttg atgtcaaata
     7501 acagtaaatc tgtatacttc atcaattctt gaaaacgtga aaagaaaggt tcttcatacg
     7561 taaaaggttt tccgcatgta tccaatgtag tatgcatctt ccgctctttt gcttttttaa
     7621 atagttcgat caaaaaatcg atctgcaata gaggttctcc accactaaca gtaataccac
     7681 ctttatctcc ccagtaaggt ctgaagcgtt ctgctttatc taaaagttca tctgctgtgt
     7741 attctttccc tccgccaata ttccaagtat ctggattgtg acaaaattgg caacgcatgc
     7801 ggcatccttg cataaacacg acaaagcgca gtccaggtcc gtctactgaa ccgaaagttt
     7861 cgatagaatg aacatagccg attgtttttt cttccattta agttcacatc ttttctttga
     7921 tttcatttta agtacaagtt agctgtcgaa gcgaagcttt ctgaacaaaa tttttcgcaa
     7981 ttgccgcttc gttatgtgta aaatttacag ggacggaaca tacaagtcct gcccctgtaa
     8041 atcgtttctt tcaaataagc atactgatta ttcgattttg atgcgatggc tattcaacgt
     8101 cctttcgacg ctaagtcgaa aaactttttt cacgaacttt ttacattctg tcgtgtgaag
     8161 ttctcatcaa gacatctaat tgttgttctc ttgtcaagtc acggaattta actgcataac
     8221 cagatacccg gatcgtcaag ttcggatatt tcattggatt ttccattgca tcacgcaata
     8281 aatcattact gaatacattg acattcaaat gataagctcc ttttgagaag tatccatcta
     8341 agacattacg caagttttcg attcttgtat cttgttcttt tccaagacct gaaggattga
     8401 ttgtttgggt atttgaaatt ccatccaatg cacagccgta attcatttta gctgttgagt
     8461 tcaggctagc taataaccca ctttgttccg cattgtaact tggatttgct cctggcgcaa
     8521 gtggttttcc agcttcacgg ccatcaggag tatttcctgt atttttaccg taaacaacat
     8581 ttgaagtaat cgtcaataat gaagttgttg gttttgagtt gcggtaagtt ttatgacgtt
     8641 tgatttgtcc catgaagtat tccaagatcc atgaaactaa ttcatctgca cggtcatcat
     8701 catttccgta tttagggaag tcgccatcca cttcaaaatc aactgctatt cctttttcat
     8761 tacggattgc ttttactttt gcatatttga tcgctgacaa gctgtctgcc gcatgagaga
     8821 tcccagcaat acctgtagca aatgtgcgtt gcaagtcaga atccatcaaa gccagctgag
     8881 ctgcttcata agaatattta tcatgcatat aatgaatgat gttcaacgta ttgacatata
     8941 gttctgctaa ccaatccatc atattcatga agttatgttt cacttcttca aagtctagta
     9001 tttctgatgt gattggacga tattctggtc caacttgggc gccagtcact tcatcgactc
     9061 cgccattgat ggcatataat agtgttttag ccaagttagc acgagcgcca aagaattgca
     9121 tgtcttttcc gacaactgtt cctgatacac agcaagcaat cgcacaatca tcagaacccc
     9181 attgttcacg aagctgatta tcattttcga attgaatcga agaactttcg atagcgattc
     9241 tgctagcata tgttctgaat ccttctggaa ggtgttcaga atacaaaaca gtcaagtttg
     9301 gttctggtga aggtcccata tttgttaatg tatgaagcag acggaaatca ttctttgtaa
     9361 ctaatgaacg accatctaat cccatacctg caatggataa agtagcccag atagggaatc
     9421 ctgagaataa ttggttatat tcaggtgtac gcgcgaattt gaccatacgt aatttcatta
     9481 ctaagtggtc gatcaattct tgtgcttctt tttctgtgat tactccatta tccaaatcac
     9541 gttggatata gatatcaagg aatgctgaga tacgtccgat tgacatcgca gcaccatttt
     9601 gtgatttgat tgctgctaaa tatccgaagt atagccattg gacagcttct tttgcatttg
     9661 cagctggttt agaaatatca tagccatatt tcgcagccat ttctttcatt tgcgctaatg
     9721 cacgatattg ttcagtgatt tcttcacgta aacgaatcac ttcttcactc attgtatgat
     9781 aaccagtcat atcatgatct tttttctttt gttccatcaa atagtcgatt ccatacaatg
     9841 ccacacgacg atagtcaccg atgatacgtc cgcgaccgta agcgtctgga agccctgtaa
     9901 tgattttgtt tttacgagca gcacgcattt cagcagtata agcgtcaaat acaccttgat
     9961 tatgtgtttt gcgccagtca gtgaaaatat gtgtcaattc atcgtttggt gtatagccat
    10021 tgctttttag cgcattgtta gccatattga ttccgccgaa cggcatgaat gcttgtttca
    10081 aaggtttgtc tgtctgtaga ccgacgattt tttccaaatc tttatccaaa tagcctgcat
    10141 cgtgtgaagt aatagttgat acgatttctg tgtccatatc atagacaccg ttacgctcga
    10201 attggatatc atttaacttt tgcaattcat cccataattt ttctgttgct tctgttgggc
    10261 cttccaaaaa gctgtcatca ccgcgatatt cagtgtagtt attttgaata aagtctctag
    10321 tgtctacgcc ttctttccat ttggtacctt taaatccatt ccattgctcc attgtatgga
    10381 cctcctaaat ttctgtttta tgtaacagtt tttacgtgat tagtataaca cagcacccta
    10441 tctttgcaag ggttttcttg ttttttattt aacaattttt atgttttatt tacatatatt
    10501 taaatcaacg ttgacatacc aacatttaag cattgtgaac actttcatct ttttttgcaa
    10561 acgtataagg atttattaaa aatgaatgaa aaagagacgc cacctgtatt aatacagatg
    10621 gcgtctcttc attaaaatac tacaacagtt acgactcgat gaaaatctca ggagcatcga
    10681 taacttcgat gacttctcca ccttttttct catccatcgc aaatgatcca ttcgatgttc
    10741 gatcacctat agggaaatca gttgggtcaa tcgttaactg ctgccctttt tgattcatca
    10801 aggttatttc ttttgatctt ccttccgaca tatagataat acggtgcgga ttttttttca
    10861 gctcacgcat gaccgtcact ccccgcttcg cacgtgaagt ttggctgatt tcttgagcaa
    10921 gcatgcgttt aacagctcct cgctgagtaa cgattacgat cggcgtatca ccttctgcta
    10981 acacaagcag cccgttgata atcctatctc catctttcaa attcatagac ttcactccag
    11041 ctgcctttgc tccaacaacg ggaacatctg ttaaaggata gcgcaaaccc attccttgat
    11101 gcgttactaa gaaaacatct aagcctggct gatcttcttt cagatacaca gcaatcagtt
    11161 catcttcttc cgcctttagc ttcatacagc tgagcggacg gctcttatag gtgcgccatg
    11221 gttcgaactc tgtcattcgt gtttgcttga tcaatccatt cttgctgatg aatacaaatg
    11281 ttttctctgc ttgaattttt tgatatggga aaacagctaa aatcgattcg tctacagaca
    11341 gattggagat tgtttgagaa atgtgctcac cagcatcttt ccatttcaaa tcgggtagtt
    11401 catggacagg gcggtagatc acatttgctt tatttgtgat cagaagaaca tggtccaatg
    11461 tactcaattc acctgagtat agcaagtagt cgccttctcg catcccgatt tcttctggtt
    11521 tggaagcact ataagaacga aggcttgttc gcttcacata gccttctcgc gtcacactga
    11581 caattacgtc ttcttgtgcc actaagactt gcgtatcaat tttgatttct tcgatttcgt
    11641 cttcgattgt cgttagacga gcggaagaat aatttttttt cacttctcgc aattcttttt
    11701 tcataacaga gaaaagctct ttgtcatttg aaaggatttt attcaactct gtgatttgcg
    11761 caatcaagct ttcggattcc ttttgaagct ctgtgatatc cgtgttcgtc aaacggtata
    11821 attgcaatgt gacaattgct tctgcttgtt cttctgtaaa ttgaaagacg tcgactaaat
    11881 tctttttggc gtcttttttg tctttacttt cacgaatcgt tgcgattact tcatccagaa
    11941 tcgataaagc tttcatcaat ccttctacga tatgctgtct tttttgagct ttggctaaat
    12001 cgaattgact acgttttgtt atgacttgct ttcgatgctc gatataactt cttaaaatgt
    12061 ctttcagtcc aacttgatgc ggagtcatat ggtcgatagc gaccatattg aaattgtaat
    12121 tgatctgtag ctcagtgttt ttgaacaaat aattcaaaat tccttgagca ttggcgtctt
    12181 ttttcaattc gacgacaatt tgcagacctg ttctatcact ttcatctcgt acttcagcta
    12241 ttccatcgat tttcttattc aaacggatct catccatttt tttcaccaag gtcgctttat
    12301 ttacttcata tgggatttct gtgatcacga tttgctgttt attacccttc attgcctcta
    12361 tctttgtttt cgaacgaaga ataacttttc ctcgtcctgt ttcataagct tttttgattt
    12421 catcttttcc ttgcaagatc cctcctgttg gaaagtcagg tccaggaata tattccatca
    12481 atttttcaag gctcgcttgt ggatgatcga tcatatagat tgtgccatcg atcacttcag
    12541 ctaagttatg agtcgggatt tctgttgcat atccagcaga tatacctgtc gatccattga
    12601 cgagaaggtt tgggtatcga gctggaagga ctgttggttc tttttctgta tcatcaaaat
    12661 tccacacgag atcgactgtt tctttctcga tatctgctag catctctcca cttaacttgg
    12721 ataatctggc ttcagtataa cgcatcgcag caggtggatc accgtccata ctcccgttat
    12781 tcccgtgcat ttcgatcagt acttcccgca acttccagtc ttggctcatg cggaccatcg
    12841 cttcataaat actgatgtca ccatgaggat gataattccc catgatattt ccgacagatt
    12901 ttgctgattt acggaatcct ttgtcatacg tattgccatc tttgttcatg gcaaataata
    12961 tccggcgttg cactggcttc aatccatcac ggatatcagg cagtgcacgt tcttggataa
    13021 tatatttgga ataacgtccg aagcggtcac ccatcacttc ttctaaagtt aattcttgta
    13081 cttcttgtcg attttccatc aggtgacctc ctattctact tcaaataact gattgttttc
    13141 atttttatct gctcgttctt catctaataa atcattagaa actgaaggag agatttcagt
    13201 atcctctttt ttatctaaga tactgccatc ttcttcgagc gtaaattgca cgtgattttc
    13261 gatccatttt cttcgaggtt ctactttgtc acccatcagt gtggttactc gacgttcagc
    13321 ttgcgcagca tcatcgatac ggacgcggat caacgtacgg gaagtcggat ccatcgttgt
    13381 ttcccacaat tgttccgcat tcatctctcc aagtccttta tatcgctgca gcatatatcc
    13441 tttgccgact tttttaatca ttgctgccaa ttcgtcatct gtccacgcat attcaatgac
    13501 ttgcttcttt ccttgtcctt tggatacttt atataaaggc ggtaacgcga tatatacttt
    13561 cccagcttca atcagcggtt tcatataacg ataaaagaaa gtaagcagta atacttgaat
    13621 atgggcgccg tctgtatccg catcggtcat gatgattact ttgtcgtaat tacagtcttc
    13681 gatagaaaac tcaggaccga ctcctgcacc aatcgtatag atcatcgtat tgatttcttc
    13741 atttttcaag atatcttgca ttttagcttt ttcggtattg atgacttttc ctcgtaaagg
    13801 tagaattgct tggaactttc tgtctcttcc ttgtttagca gatccgccag cagaatcccc
    13861 ttcgactaaa tacaattcat ttttcttagg gtttcgggat tgtgcgggtg tcaattttcc
    13921 tgaaagaaga gattcgcctt ttttacgctt cttcccattt cggctttctt ctctggcttt
    13981 acgtgctgct tctctcgctt ctctggcttt gattgcttta cggaccaaca tctggctcat
    14041 ttcgctattt tcctgcaaat aaaagcccat ttgttcactg atgacgttat ctactacggt
    14101 acgagcaact ggcgtaccaa gtttttcttt tgtttgccct tcaaattgca gaagattttc
    14161 aggtacacgg atagaaagta cagcagctaa cccttctctg aaatcactac cttccagatt
    14221 cttgtcccgt tctttcagca aaccaacttt tctcgcatat tcattataag atttcgtcat
    14281 cgccgctttc ataccagctt catgggttcc cccatctttt gtccgaacgt tattgacaaa
    14341 agataaaaca ttttctgaat aaccgtcatt gtattgataa gcgacctcta cttcgatacc
    14401 ttctttttct cctgaaaaat aaacaactgg tgtcaatgta tctttttctt cattcagata
    14461 atcaacaaat tctttgattc cttcttcgta atgaaaaatt tccttgactg gttcttctcc
    14521 acgaagatca gaaagagaga ttttaacgcc ttttagaaga aaggcagatt ctcttaaccg
    14581 ttcggctaaa atttcatatg aaaatttcgt tgtactgaat attgtatcat ctggtaaaaa
    14641 atggacagtc gtcccgtttg ctttttttgt ttttcctatc tttttcaaag taccgtcagg
    14701 tttaccgccg tttttaaatt tctgctgata ctctacgcca tctctaacga tcgttacaat
    14761 tagccatttc gataaggcat ttaccacact ggctcctaca ccgtgcagtc caccagaagt
    14821 tttgtaccct ccttgaccaa actttccgcc agcatgcagc acagtaaaaa tcacttcaac
    14881 tgttggtatt ccggaagcat gcattcctac aggcatccca cgtccgctat ctgcaacagt
    14941 tatactattg tcttcatgga tggtcacatc aatctcactg ccataacctg ataatgcttc
    15001 gtcaactgca ttatcgacga tttcatagac taaatgatgc aagcctcggc tgtcagtgga
    15061 tccaatgtac atccctggtc tttttctaac tgcctctaat ccttccaata cttggatgga
    15121 cgagtcattg tattcgttgt ttactttttt tgccaaggca aacgctcctt caataatact
    15181 ttgcggcaaa accgcaccac gattatcata ctagaaaagg tcgtgaaaaa aaagagggta
    15241 gacgagaaat caattttaat tatctcctaa ccgctttttc gacattcccc tctatcgatc
    15301 atctcttctt tgtggtcatt tttatttttg gttttctttc aacttaccaa gttcaatctt
    15361 aatacaacga tccattacga tatctgtacg tcctgctttt cttagcatct cagctgcttc
    15421 tttgttttca atccctaatt gtgcccagaa cactttagca tcagttttca aaaaatcacc
    15481 tgctatttca ggtaaaaatt cactccgtct aaatacatct acgatatcga ttggatcatc
    15541 gatttctgtc aaattcgcat agactttctc gcctagaatt tcttcaccag ccaataatgg
    15601 atttactggt atgattttat atccattgtc ttgcaatacc tgtgcaacca tgtaacttgt
    15661 tcgttctggt ttattgctca agcctacgac ggcaatattt tttgcttctt ccaaatagtt
    15721 gaaaatcgtt tcttgtgttg gattttttac agacatttct gccacccctt gcttttcttt
    15781 ttattccatc tattatactt gtatctttca ttttttcaat tgttcctgtt acctattcat
    15841 gctaaaataa aggatagttt tcaacttgaa agagggtaaa aaatgaaatt gatcttatta
    15901 cttattttag catatctttt aggttctatt ccttccggtg tctggattgg caaattgttt
    15961 ttcaaaaaag atatccgaca acacgggagc ggtaatactg gaacgaccaa tacatttcgt
    16021 gtattaggca aaacagccgg gattgttgtc ctgcttatgg atattttaaa aggtacattg
    16081 gctacttgtt tgccgatgat cttccacctt tctgggatta accctctttg gtttggggta
    16141 tgcgccattc ttggccatac atttccgatt tttgctgggt tcaaaggagg aaaagctgtt
    16201 gcgacaagtg caggaatgct tttaggattt aatcctgtat tttttgttta ttcttgtatc
    16261 atctttatcg ttagtttatt ttgtacaagt atggtgagtt tgagcagtat gatcagtgca
    16321 gtattgatca ctttatctac gatcgtcctt ccttatgtag ctcctgtgat tttggctaag
    16381 cccaattggt tactgacgat cattgccttt ttagtttctt cctttatctt ttatcggcat
    16441 aaagacaata tcaagcgtat cagaaaccat actgaaagtc gaatcccgtt tggtttgaat
    16501 agcagcaaga aaaaatcctg atactttttc attccaaaga gataaaacgg gagtgtgaca
    16561 aatcgatgtc acactccctt attacttaca ggatagcttt tttacacacc ttggtaaacg
    16621 gtgttccctg ctaacttctc ggtcacaacc tcttacttca ctgtgatgga atattttgtc
    16681 ttgaagattt catgggcacc tagttgattg atccctactt tttccatcaa gtttccacta
    16741 ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac aaacgaaagg cgcttctttt
    16801 ggataaggag accagatccc tacataaggc atatctttat agcttaaagt tacgctatgg
    16861 gaagattttt cacttcgaat ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca
    16921 tcttgctcaa aaagttcacg attcaaagcc agattcgtat ttgtctgacc taacgtcttt
    16981 tgatccatat cgatgaatgg gcctttcaat ggtaggcgta atcttgattt caatggagaa
    17041 aatgccagat agaaatcttc aaatgacaat ccttcttcaa gaggtacatt gaaagcagga
    17101 tgtccaccga tagaaaagaa catttctttt tcaccggtat ttgccacttg ataatgaacc
    17161 gtgatgccgt ctcctcctaa ttcatagctg atcactaatt caaaatcaaa tggatacttt
    17221 tctaatgtct ccggcgtact ccttaagatg aaactagcca tttcttgtcc atgttcaatg
    17281 acttcaaaat ccatatcccg agcaaatccg tgttgcccca tttgatatgt tttcccttga
    17341 taaacatact gatcatcttt taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg
    17401 ccccaatacg ctggatcacc ttgccagata tactctaaac catgttcttt tgatttcaga
    17461 ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca gctggtcatt ttgaataata
    17521 acagtcattg ccattcctcc aacttcgact tactttttaa aatgggaagg ctggaacaaa
    17581 catttctatc tgccccagtc cccttacttc taataactgt gttttgctaa ctcgtcttta
    17641 aaacgggaat tcaatacttt taaataagtt cccttcatgc ctaacgaacg agattcaatg
    17701 attccagctg attccaattt tcgtaaagca ttgacgatca cagaacgagt gatgccgata
    17761 ctatcggcaa tattagaagc agtcagacga ccttcatctc catccaatgc ttcaaagata
    17821 gcttgtacag ccttcaattc gctataagat aatgtgttga ttgccatttg gacagccgtt
    17881 gcactccgta catctgcttc gatgcttcga gacttctgat aaaggatctg cataccgaca
    17941 accgtagctc catattctgc taagactaga tcgtcatctg caaacgattg ttccatacga
    18001 gataagataa tcgtacccaa acgttcgcca gctccaaaga tcggaacgac agtcgtcaga
    18061 ccaaatggat atttctctct tgactctacc ggaaatgcag taatatcact agtaattggg
    18121 atattagctt cagttttcat caactgtgcc actgtatcag tataagattc aggaaattgt
    18181 ttctcaacaa acattgtttt gattcgttca ttgttcacat cgtgacgttc attataacct
    18241 agtaatactc cgtcacttcc tatgatatag gtgttgctgt caagaatacc acctagtgtg
    18301 acagccattt tatcgtaagg caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa
    18361 agtgtgttta tcttgcgggt tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt
    18421 ataaaatata gcgacttaaa tcttcatttg ccgcaattga gccaagtttg tcatccacat
    18481 atgcttctgt gatcgttatt tctcccattt gcatatctgg tgcttcaaat aatagatctt
    18541 ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt
    18601 tgacatcgaa agcgatttga gcgatccgtt caattgcttc ttttgtaaac gtaactgaca
    18661 cattttctgt accgatcaac gcgacgtatt gttttactaa agcattattt ggttcggtca
    18721 gaatcttcac aaaatcttca gctgtcaaat catctaattc tacccgtatc gggaagcgtc
    18781 cttgaagttc tggaattaga tcacttggtt tagacaaatg gaaagctcct gaagcaatga
    18841 ataggacgtg atctgtttgg atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa
    18901 ttggcaatat gtctctttgg acgccttctc tggatacttc tccagattgc tgtgatttag
    18961 aagtgatctt atcgatttca tcgataaaaa taattccact ttcttccgct aatttgattg
    19021 cctcactatg gatatcagca tccttgacta attttgctga ttcttccttg atcaataact
    19081 ctaaagcttc tttgactgtt accgtccgtt ccactttttt cttcggtgtc aaagcaccta
    19141 gagtttcatt taggtcgatc cccatttgct ccaatccatt gttcatcatt ggtgcaggtt
    19201 tcttttggtc ttcaacttca atggttactt cacgactatc cagtaaaccc ttttgtagtt
    19261 gttccaagac tgtacggcga ttgattcgaa tatcttctgt cacttcttct tgcggttcag
    19321 cggtattttg ctgactagca ttaaaaagat tcatcatttg ttcaaattga ttagtggatt
    19381 gtttctgttc ttttttgata ccaggtacca agatttttac caaacgtttt tctgcatttt
    19441 ttttagcact tgtgtataca cggctgtact gctctttttc tacgatttgg atcgcatttt
    19501 ctactaaatc ccgaaccatt gattctacat ctcttccgac ataaccgact tcagtaaatt
    19561 ttgttgcttc gactttgaca aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt
    19621 ctgttttacc gacccctgta ggtccgatca tcagcatatt ttttggtgta atatcttgtt
    19681 gcattttttc atctaactgc aaacggcgat agcggttacg caaagcaacg gcaacagatt
    19741 tttttgctgc gtgctgtcct acgatatatt catctaattc acttacgatt tgtcttggtg
    19801 tttgttttaa ttcatacata tctactcacc tcttacagtt cttctacgat aatattatga
    19861 ttagtaaaga tacagatatc tgcggcaata tttaacgcat tttctgcaat ttctttcgca
    19921 ctcatgtcgt tttgagcata gtttttcatt gctcgggctg cagccaacgc aaaattccct
    19981 cctgatccga tagctaagat accatcatct ggtgtgataa cttctcctgt acctgaaaca
    20041 agaagcattt ctttatcatt cattacgatc agcatcgctt caagtttttg catagattgc
    20101 tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt gtagattccc gttatattcg
    20161 tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa
    20221 cctacaacaa cttctccatt gtagatacgt cgtactttct ttgcagtgcc tttcatgaca
    20281 acagattcac ccattgttac ttggccatct ccagccatag caaatttccc gtctttttcg
    20341 acggcacaga tagtggttga atgaaatggt gattcgacca tgtttgtttc ctcctaaatt
    20401 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
    20461 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
    20521 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
    20581 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
    20641 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
    20701 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
    20761 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
    20821 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
    20881 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
    20941 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
    21001 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
    21061 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
    21121 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
    21181 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
    21241 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
    21301 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
    21361 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
    21421 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
    21481 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
    21541 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
    21601 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
    21661 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
    21721 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
    21781 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
    21841 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
    21901 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
    21961 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
    22021 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
    22081 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
    22141 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
    22201 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
    22261 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
    22321 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
    22381 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
    22441 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
    22501 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
    22561 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
    22621 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
    22681 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
    22741 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
    22801 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
    22861 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
    22921 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
    22981 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
    23041 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
    23101 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
    23161 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
    23221 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
    23281 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
    23341 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
    23401 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
    23461 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
    23521 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
    23581 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
    23641 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
    23701 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
    23761 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
    23821 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
    23881 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
    23941 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
    24001 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
    24061 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
    24121 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
    24181 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
    24241 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
    24301 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
    24361 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
    24421 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
    24481 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
    24541 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
    24601 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
    24661 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
    24721 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
    24781 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
    24841 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
    24901 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
    24961 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
    25021 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
    25081 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
    25141 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
    25201 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
    25261 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
    25321 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
    25381 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
    25441 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
    25501 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
    25561 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
    25621 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
    25681 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
    25741 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
    25801 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
    25861 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
    25921 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
    25981 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
    26041 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
    26101 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
    26161 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
    26221 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
    26281 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
    26341 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
    26401 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
    26461 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
    26521 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
    26581 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
    26641 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
    26701 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
    26761 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
    26821 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
    26881 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
    26941 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
    27001 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
    27061 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
    27121 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
    27181 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
    27241 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
    27301 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
    27361 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
    27421 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
    27481 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
    27541 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
    27601 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
    27661 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
    27721 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct
    27781 atatatgaat ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac
    27841 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
    27901 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
    27961 gaagaagctg gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat
    28021 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
    28081 ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa aatttgctca aattgcaaaa
    28141 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
    28201 ttagaagtcc aagatctaga cggaaataca ttagaacccg tat
//
LOCUS       DACDHL010000028        27923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDHL010000028 DACDHL010000000
VERSION     DACDHL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.028"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            199..1458
                     /gene="lysA"
                     /locus_tag="IV228_001266"
     CDS             199..1458
                     /gene="lysA"
                     /locus_tag="IV228_001266"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8717195.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1445..2245
                     /gene="dapF"
                     /locus_tag="IV228_001267"
     CDS             1445..2245
                     /gene="dapF"
                     /locus_tag="IV228_001267"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8717196.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2248..3129
                     /locus_tag="IV228_001268"
     CDS             2248..3129
                     /locus_tag="IV228_001268"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8717197.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3126..3842
                     /locus_tag="IV228_001269"
     CDS             3126..3842
                     /locus_tag="IV228_001269"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8717198.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3848..4543
                     /gene="dapD"
                     /locus_tag="IV228_001270"
     CDS             3848..4543
                     /gene="dapD"
                     /locus_tag="IV228_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8717199.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4559..5560
                     /locus_tag="IV228_001271"
     CDS             4559..5560
                     /locus_tag="IV228_001271"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8717200.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5557..6918
                     /locus_tag="IV228_001272"
     CDS             5557..6918
                     /locus_tag="IV228_001272"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8717201.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6878..8029
                     /locus_tag="IV228_001273"
     CDS             6878..8029
                     /locus_tag="IV228_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8717202.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8004..9173
                     /locus_tag="IV228_001274"
     CDS             8004..9173
                     /locus_tag="IV228_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8717203.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9348..10727
                     /locus_tag="IV228_001275"
     CDS             9348..10727
                     /locus_tag="IV228_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8717204.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11008..11805
                     /locus_tag="IV228_001276"
                     /pseudo
     CDS             11008..11805
                     /locus_tag="IV228_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11994..12662
                     /locus_tag="IV228_001277"
     CDS             11994..12662
                     /locus_tag="IV228_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8717205.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12862..13092)
                     /locus_tag="IV228_001278"
     CDS             complement(12862..13092)
                     /locus_tag="IV228_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717206.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13219..14601)
                     /locus_tag="IV228_001279"
     CDS             complement(13219..14601)
                     /locus_tag="IV228_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8717207.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14757..15944)
                     /locus_tag="IV228_001280"
     CDS             complement(14757..15944)
                     /locus_tag="IV228_001280"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8717208.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15951..16982)
                     /locus_tag="IV228_001281"
     CDS             complement(15951..16982)
                     /locus_tag="IV228_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8717209.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(17028..18452)
                     /locus_tag="IV228_001282"
     CDS             complement(17028..18452)
                     /locus_tag="IV228_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8717210.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            18627..19532
                     /locus_tag="IV228_001283"
     CDS             18627..19532
                     /locus_tag="IV228_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8717211.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19623..20300
                     /locus_tag="IV228_001284"
     CDS             19623..20300
                     /locus_tag="IV228_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8717212.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20581..21477
                     /locus_tag="IV228_001285"
     CDS             20581..21477
                     /locus_tag="IV228_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8717213.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21532..22902)
                     /locus_tag="IV228_001286"
     CDS             complement(21532..22902)
                     /locus_tag="IV228_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8717214.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23140..23886
                     /locus_tag="IV228_001287"
     CDS             23140..23886
                     /locus_tag="IV228_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8717215.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23883..24722
                     /locus_tag="IV228_001288"
     CDS             23883..24722
                     /locus_tag="IV228_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8717216.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24723..25673
                     /locus_tag="IV228_001289"
     CDS             24723..25673
                     /locus_tag="IV228_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8717217.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25814..26566
                     /locus_tag="IV228_001290"
     CDS             25814..26566
                     /locus_tag="IV228_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717218.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26585..27736
                     /gene="queG"
                     /locus_tag="IV228_001291"
     CDS             26585..27736
                     /gene="queG"
                     /locus_tag="IV228_001291"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8717219.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9170 a         5058 c         5851 g         7844 t
ORIGIN      
        1 gtttttatgt ataaatagct tctaatagtt cgggtatttt gtttttataa ccatttttat
       61 tcttattttt aaaaaaatat ttcgaaatat actagacaat tcagaaaatt ttgtttaata
      121 ttattccaat aaaattcaaa tctttatcaa atgaaattcc aataaaaacg gaaaaaaagt
      181 gaaaagagga atgaaaaaat gacagaattg ataattggcg gagtcacagc aagcacattg
      241 gcagaaaagt ataagacacc tctgtatgtg tatgatgaag aaaagatgag acaaacgatg
      301 ctgtcattca agcgaggctt tgtatcgaaa cgttttgata caaaagtgct ttacgcctct
      361 aaagcattcc aaacgattga gatgctgcat ttagttcgtg aatatggatt cgggctagat
      421 gttgtcagtg gtggggaaat ctataccgct ttgcaagctg attttcctag agatactatc
      481 tacttccatg gtaacaacaa gacgccagag gaactgatct atgcacttga aaatgatttg
      541 ttgcatattg ttgccgataa tttgatggaa gttgaattat tagcagaatt aagccaacgc
      601 tatttaaaaa aaatctgtat catgcttcgg ttgaatgtcg gagtagaagc acatactcat
      661 gaatatatcg tcactgcgca tattgattca aaattcggca tgagctatga aagtaaagag
      721 tgccaacaag cccttcagtt gataaaagaa agcagctatc tagagttaga aggatttcat
      781 gctcatatcg ggtcacaaat ttttgatgtt actgcttggt tagctgaaat cgacaaattg
      841 acgaattact taaccgattt tgaccaacct ctttccttga atcttggcgg agggtttggg
      901 attcgttata cacaaaatga tcagccgatc ccagtcgaag aggcactaaa aaaactgatt
      961 gaatatacgg aagaagcttt ggaaaaacga aaactgacca ttcgtcaatt actgatcgaa
     1021 ccgggtagaa gtattgttgg agaagctggt acaacactat acacgattgg tttcatcaaa
     1081 aagacaccac ataaaaaata ttactttgta gatggcggga tgacagataa tatccgtcca
     1141 gctctgtatc aggcagaata cgattgtgat ttagcagcaa aactgtcgga agaaaaaaca
     1201 gagaaagtga cgattgcagg aaaaatgtgt gaatcaggcg atgtggtaat taaagaagct
     1261 tatttgccga aagcagaatc gggtgatctt ttagctgttt attccacggg agcttatgga
     1321 tactcgatga gcagcaacta caatcgagcg acacgaccag cagttgtttt tgtcaataat
     1381 ggccaatcaa gactgattgt tcgcagacaa gcattttctg atctactaag gggggaagtt
     1441 tcctatgatc cttcataaat ataatggctg cggaaatgat tttatcttat tagattacgc
     1501 agaagacaca gattatagtc aattagccgc ttctttatgc acaaaagaaa aatatgatat
     1561 agacggattg atcgccgtaa aaatcgatcc gttggaaatg atctattaca acaaagatgg
     1621 cagccgcgcg ccgatgtgcg gaaatggcat tcgctgtttt gctcgatacg taaatgatca
     1681 gaacatgatg aatgaacagg aattcaacgt agagacatta gcaggcacaa gaaaaattac
     1741 ggtgttggat ccctcacctt tttattgcag cgtagatatg ggagtgccag atgattctcc
     1801 ggaaaaaatc aaagctaatc aaacttatcc aataaaaaac caggaaatag agatcaatgg
     1861 tgaaaaagta acgatcacta gtttattttt aggaacgatt cataccgttg tttttgttga
     1921 aaatgcgcgt gaggaagtct ggagaaaaag aggtgaactg atctgtcatc atcctttgtt
     1981 caaagaaaaa accaatgtca actttgtaga agtagtcaat gaacaagaat tgatcgtccg
     2041 tacctatgaa agaggagtag gttggacact tgcttgcggt acaggttgct gtgcctctta
     2101 tgtggttgct aaggaaactg ggaaaatttc agcagatcag gtagttgttc acttggaaca
     2161 aggagacttg atgatctctg gtgaggatgc gatacaaatg gctggcccag cagttcatga
     2221 atggacaaga atattggagg aatgaagatg ataaaaggtt cgatcgtggc gctgatcacg
     2281 ccgatgaatg aagagggatc agtagattat gctggtttgg aaaaattgat ccaattccat
     2341 ttagatgaac aaacagatgg attattagtt ttaggtacaa caggagaatc ttctacgctc
     2401 acacaatctg aggaggagca gatccttcaa ctgacagtaa agaaagtggc tggccgtgtg
     2461 ccggtgattg caggagctgg aacgaataat acgaaagaaa cgattgaaaa agcgaagcat
     2521 tttgcttctt tgggggcaga cgcacttttg gtcatcaccc cttactacaa caaaacaagt
     2581 gatgcaggac tagctgctca ttttacagca attgcagaag cctcaccgat tccgcttatt
     2641 ttgtataatg ttccttctag aacaggcatg tcgattccaa tccatgtatt agtaaattta
     2701 gcagaacatc ctaatatcat tggtttgaaa gaagcctcag gagatatggc ttatgtgatg
     2761 gatgccgctc ggttgattgg agaagaattc ttcctttaca gtggaaatga tgatttgatc
     2821 ctaccagtaa tgagtgtagg aggaagcggt gtaataagtg tatgggcgaa tatccaacct
     2881 aaaatagtcc atgaattagt aaaagatacg caggacggaa gatggcagca agcaaaagaa
     2941 aagcaattaa atgcacttga actgatccat gccttgttca gtgagaccaa tcctattcct
     3001 gtcaaagcag ccatgtcatt attagactta ccttccggac ctctacgcct gcctttagtt
     3061 tctctttcaa aagagaaaaa gaaacaatta gctcagcttt tgttaaaaga aaggacggtt
     3121 gaaaaatgaa aatcggactg attggcagtg gacagatggg aaatagagtc gaagaagttg
     3181 cacaagaaag aggagatacg gtccttcttt tacggacggc acctaaagaa gcaactacgc
     3241 ttgcttttac ggaactgcca gaactgctta ttgatttttc tcatccagat aatttagaga
     3301 aaatcctttc gtatagctta tcatatcaac ttccgttagt cattggcacg acaggttata
     3361 cacaatcaca gttccaagaa ataaaagaac atgccaaaca tgtacctgtg atgtatagtg
     3421 ctaatttttc gttcggcatc atggtgatga atcaactaat caagcaagca gctgcaatgt
     3481 tgcaaggctg gcagatcgaa ctgattgaaa aacatcatag tcgtaaaaaa gatgcaccat
     3541 ctggtactgc aaatatgctg cttcaaacaa tccaagaagt gcaaaagctg cagcccgtat
     3601 acaactggca agggaaaaaa agagaagaaa accaaattgg tgtacacagt attcgtgcag
     3661 gttctttacc tggtgaacat gatgtattgt ttgctgctac tgatgaaata tttacgatca
     3721 aacatgaatc tttttctaat cgaatctttg ctgcaggcgc agtagaagca gcggactggt
     3781 tgaaagatca gtcaccaggt tattacaaat tagaagattt attaatggac aaaggggtgt
     3841 aaaaaacgtg gaagctcaag aaatcatccg ctacattcaa acggcatcta aaaagacgcc
     3901 tgtgaaagtc tatctgaatg taaccgagcc aatcgaattt aaagaaagta aagtttttgg
     3961 tgaaggaagc agcttcgttg tttttggtga ctatgagacg attgctccgg ttttagaagc
     4021 tgaatcagaa aaaatcattg aaatcgaaat cgaaactgct gcaagataca gcgcagttcc
     4081 tttgctagat attaaaaaaa ttaatgctcg tatcgaacca ggtgctatta tcagagatca
     4141 agtgtcaatc ggcaacaatg ccgtgatcat gatgggagcg attatcaata ttggtgcagt
     4201 aatcggtgaa aacacgatga ttgatatggg tgctgtctta ggtggacgag cgacagtggg
     4261 caaaaactgc catattggtg caggagctgt cttagcaggt gtgatcgaac cagcatcggc
     4321 caaaccggtt atcgtagaag acggcgtact ggtaggtgcg aatgcagtga ttgttgaagg
     4381 tgtccatatc ggaaaagatg cagtcgtggc agcaggagca gtggttttag aagatgtggc
     4441 agcagaaact gttgtcggtg gaatacctgc acgtgtatta aaaataagtg atgatcagac
     4501 aaaagaaaat acagcattga ttgctgcgtt acgcgaatta tagaggagat ggagaaaaat
     4561 gcgcgaaata gatacctgta ttgtgggggc aaccggatta gtcggccaaa cgatgctcaa
     4621 agtgttagaa gaatatgatt ttccagtagg acgattgaaa ttattagctt caaaacgttc
     4681 agccggtaaa gtttgtgtat tcaagaaaaa gaaacacact gtacaggaat tgacagaaac
     4741 ttcatttgaa ggatatgatt tagcgctatt ttctgcaggt gcttctgtat ccaaagagtt
     4801 tgcgccaatc gcacgaagtc agggactatt tgtcatagat aattcctcag catggcggga
     4861 agattcggat attccattaa tcgttccaga ggtcaatctg gaagactata cattgaatcg
     4921 gctgattgca aatccaaatt gttcaaccat tcaagcggta cttccgctta aagctttaca
     4981 agaagccttt ggtatccagc gagtcaatta ttcgacctat caagcagttt ctggttccgg
     5041 gcaaaaaggc attgaagatc ttcatcgtac gcaaaatgac gaggaaccag ttttgtatcc
     5101 ttataatatt agccaaacgg tgattccgga aatcgatcag cctttggaaa atgggtatac
     5161 aaaagaagaa cagaaaatga taaatgagac ccgtaagatt ctgcatcagc ctgctttgcc
     5221 agtatcagca acctgcgtac gtgtgccaat cgaaaatgga catggagtct ctgtggcagt
     5281 tcaactgcaa aaagagttta cgacggaaga aattagagag tgtctcagcc agtttccagg
     5341 gattcaatta gaagatgatc tagcagcaca taaatatccg acgagcattc ttgctagagg
     5401 aacagatatg gtttatgttg ggaggatcag aaaagacact tctttagaga acggattgtt
     5461 gttctatacg actgccgata acatacgcaa aggagccgct gcgaatgccg tacagattgc
     5521 tgcagcttta atgcagaaaa tagcggaggt ggcaagatga gtaatacgct gaaagtagcg
     5581 aaattcggcg gaacatcgat gtctcatgaa gagcaattca aaaaagtccg tacaattgtt
     5641 ctttcagatc ccaatcgcca agtgatcgtt gtttctgctt taggcaaacg tacgtcagat
     5701 gatgacaaag taacagactt actttatttg atttatgctt accttcagca tcatgttgat
     5761 tggacacccc tttggaaaag gatttgtgat cgtttcattc aagtaagaga tgttcttgcc
     5821 ctgcagttac ctattgaaga tgaactagca cgcatacaga aacaactgga agacgaaaag
     5881 ataactgaag attatttagt aagtagaggt gaatatctga ctgctcaact gatggctgag
     5941 tatcttggtt accagtttat tgacgcaaaa gacctgattt tctttgacta tgatgggaaa
     6001 atcgatcagg aaaaaacagc acaggaaatc acaaaagcat ttaaaagata tccgaaaatg
     6061 gttgttccag ggttttacgg ggcgaaccct gcaggaaaag aaaaattatt gggccgtggg
     6121 ggaagcgaca ttaccggcgc gatactggcg aagatgttgc aagcggaaag ttatgagaat
     6181 tggacggatg tttcaggaat catgatggct gatccgcgaa tcatcgatca tcctaaaatc
     6241 atcgaggaaa tctcttttag agaactgaga gagatggctt atatgggggc taacgttctt
     6301 catgaagcag ctgttttccc agttcaagag gctgatatcc ccatccaaat aaaaaatacc
     6361 aatgatcctt tcgctcaagg gacaaaaata tcgaatcacg aaatagaaaa ggaaaatgga
     6421 ctgacaggaa tagctggacg caaggatttt ctttccatca ctttattcaa acgtcatatg
     6481 tccgatgaaa tcgggtttat ctggaaagca atgagcattt ttgctaaaca tgagatatca
     6541 atcgaacata ttccttcagg gatcgacaat atcggagtga tcgtctcagc agaagcgatt
     6601 gccgataagt tatttctcat cacgaaagaa ttaaaagaaa cacttggcgt ggaagaaatc
     6661 gtcgtgatcg aagacttagc tttgatctct gtcgttgggg gtccgcataa agaattgatt
     6721 ggattatctg ggaaagtgtt atcgatattg aacaaattag aaattcgcac atcgatctta
     6781 tcacaaggag cacaggagtt gaatctgatc atcggtgtac cgaataatca atatgaaaca
     6841 gtagtgaagg gtatctatga agggatggtg aatacggatg ctgaaagaat atcgaatggc
     6901 actccatcaa attcctgaaa tcgggtttca tgaattcaag acaaaaaaat atttatatga
     6961 acaagtaaaa aacagcgagg ccgttatcca tgaaatcggc gaaacaggac tactccttta
     7021 ttttgataat cagcaagaag aaacgattgc ttttcgaaca gatatcgatg cactgccagt
     7081 caaagaagaa acgaatctct cttttgcttc taagcatgaa ggttatatgc atgcatgtgg
     7141 tcatgacgga catatggcga tgcttttagg gttgactgac tttataacta aacacattga
     7201 ggatcttatt tataatgtag tactgatcta tcagccatca gaagaaattt ccggtggcgc
     7261 acaaagcgtt attcagtcag gattgttgga taaatatgaa gtacaagcaa tatttggctt
     7321 tcatctatgg cctggactag ctaagggaga agttttctct cgaccaggac ctttgatggc
     7381 acaaagcagt gaaacagaca ttatcattaa aggccagtct gcacatattg cttctagtga
     7441 aaaaggaatc gatagtctgg aggcagctgt acgatttttg aagaatgttt atgattttga
     7501 ccggcaatta ccggcagagt ggaatcatct aatgaaattt ggccaaatcc aaggtggaac
     7561 gatccgaaat gttttagctg gagaagttat tgtatccgga agtatccgtt cttacagttc
     7621 agatatgcaa aattatttga agaggggatt agaccattta gcagaacagt ttcactctac
     7681 agctactatt tcttttcgct ataatgacgg ctatccagct gtaatcaacc atagaggctt
     7741 atatgcagca ctgttagatt caaacaaact acatgaatta gatgctccag ttcttcaagc
     7801 cgaagatttt ggcatatata cgcaacaata tccatgtgta tttttctttt taggtgtcgg
     7861 tgatacccct gctttacatg atccgacgtt cgattttgat atggatgttt tagaaaatgg
     7921 tgtagaatgg tacaaaacga ttctttttac agaaaatctt cttgtaaaaa tcgatagcag
     7981 ttcaaaagag gagcgagtaa ataatgatga atttatccaa tcgatttagt cctaggcttt
     8041 ctagaataga agtatcaaaa atacgacaat tcgatcagca aatatcgtcg ataccagatg
     8101 tgatcaaact caccctggga gaaccggatt ttccaactcc tgaacatgtc aagcaagcag
     8161 gaatagcagc gattgaagaa gacttttctc actataccgg aatgagagga ttagaggaac
     8221 tgcgggaagc ggcttgtata tttcagcaac agcgttatgg attaacttat gatccgcaaa
     8281 cggaagtttt gacgacagtt ggtgcaacgg aagcgattgc ctctgcgttg ttatctgttt
     8341 tggaagaagg agacaaggtt cttattccag cgcctgctta ttcaggatat cagccattag
     8401 tggaactagc tggtgctgaa ttgatcccga tagatacttc tgacacaggg tttgtctgtc
     8461 agccggaaca atttgaacga gcatttgaac aatatggatc agctgtcaaa gcagtcattt
     8521 taaactatcc taacaatcct actgggacga cattgtctgc aaaacaattg aaagcaatcg
     8581 ctgaggtttt aaaaaaatat ccggtatttg ttataagtga tgaagtatat gctgaattga
     8641 cttatagtgg aacgcatatg tccatagcta cttatcttcc agaacaaaca atcgtgataa
     8701 gcgggttatc caagtcacat gcgatgactg gttggcgagt gggctttatt tttgctcaaa
     8761 aagcccttat tgacgaacta attaaagtcc atcaatatct ggtgactgcc gctacaacga
     8821 tgtcgcaaaa agctgcggct gaagcgctaa taaatggggt gaaagatagc gagaatatga
     8881 aagagcaata tcaaacacga agagactatc tggtccagca attggcgcct ttaggatttg
     8941 aagtgaccca gccaaacggt gcattttact tgttttgcaa actcccagct gccattcaaa
     9001 ctgactcttg gcagttttgt gtagaattag cggaacaggc aaaagttgcg tgtatcccag
     9061 gaattgcatt tggcccagaa ggagaaggtt atatccggat ttcttatgct agcagcatgg
     9121 aaaaattaca tgaggcctgc aaacggatca aagacttttt aacagaacag tgattcatag
     9181 attttatcaa aaaaacaatc gttaattttg cggtatattc tgcaaggaga cgattgtttt
     9241 ttaaatttag ttcaaatgaa cgcgtttctt ttgattcata actaatgtaa tgttaagttt
     9301 aaattgttta gaatgatttt aaaagaatat tcaaggaggt ttcatcaatg gaaaaaatca
     9361 aagttgtcat tgtaggtgca tctcatggag ggcatcaatc gatcttggaa ctattgacac
     9421 gatacggtga aaatgtagat atcacgctat ttgaagcagg agactatatt tccttcatgt
     9481 catgtggtat ggagctgtat ttagaagaca aggtaacaga tgtaaatgat gtacgtaatt
     9541 tcagaccaga aaactttccg caatccaatg ttcatatttt aaataatcac gaagtaaaaa
     9601 cgatcgatgc cgataaaaaa acagtaacgg ttactagcgc atcagataac gaaacaaaag
     9661 aatatcctta tgacaaattg attttgagtt ctggtgtcaa accaaacaat ctgccagtac
     9721 caggagcaga tctagaaaat gtctacttga tgcgtggata caattgggca accaaaatca
     9781 aagaaaaatt aaatgatccg gctattaaaa aaatcgctgt cgtaggtgcg ggttacattg
     9841 ggatcgaagc agcagaagca agccgaaaag ctgggaaaga agtcgtattg cttgatgtga
     9901 tcgatagacc tttaggcact tatttagatg ctgagatgac agatatttta gaacaacacc
     9961 taaatgaaaa cggtattgaa gttttgacaa atgcaaaaat cgaagctttt actggtaacg
    10021 gaaaagttga ggcaatcaaa acaagtgaaa aagaaatccc agcagatcta gtcattcaag
    10081 ctgccggtgt gaaggcaaat acagaatggt taaaagggat cgtcgactta gatgaacgtg
    10141 gctggatcca aacaaatgaa tacttgcaaa cgaatcttcc agatgtgtat gcagtaggag
    10201 atgctacact tgcttattct atcccagcac gcaaaaaaat gccgatcgct ttagcgactg
    10261 ttgcacgtag agaagcacgt tatgtagtaa aacatctttt tgaagaagta ccaaacaaac
    10321 cgttcggcgg agtcgtaggt tcctctgcat taagtgtctt tgactatcat tttgcagcaa
    10381 gcggattgaa tagctttaca gcagcaaaag caggagtaga tgtacgagtg tcttattatg
    10441 aagatacgtt acgaccaaaa tatgtaccag aagaacaagg aaatccaaaa gtgtccatcc
    10501 aattattctc ggatccaatg acccatcaat tgctaggagg agcagtatta tctacctatg
    10561 atgttacagg tcaaggaaat gtattggcac tagcgatcca aaacaaactg actttagaag
    10621 aactagcaga agctgacttc ttcttccagc caggctttga ccgacaatgg agcatgttga
    10681 atctagccgc tcaacaagcg ttaggagaag aaccatttgt agaataagaa aacgaaatat
    10741 ttgaaaagag gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa
    10801 cagcttctta tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac
    10861 cgtttatttt caaaaaaggg actgtgacac gatttgtgta ccgaccccca aaagttagac
    10921 caaaaaatct aacttttgga ggtcggtatt ttttatggtt aaatatactt ttgaacttaa
    10981 attaaagatc gttcatgatt atttggatgg caaaggcgat tggaccgtaa aaatccagat
    11041 caagagattg aagcggaaat gcttaaaatt cgggagaagc acaaggatta tggctgttta
    11101 cggatgacca atgaactaag aaaccgcggt ttctctatca ataaaaagaa agttcagcgc
    11161 ttgatcaaga agctaggaat aaaagtaacg gcatacacgc gtaaatcacg tcgctacaat
    11221 tcttatcgag gaaaagttgg agcagtagcc aaaaatcgga tccatcgtcg tttctacacc
    11281 agtatctgcc atcagaaaat cacgacggat acaactgaat ttaaatacca tgaagtggat
    11341 gccaaaggca ttatccgtca aaagaaattg tatctagatc cttttatgga tatgttcaat
    11401 agtgagattt tatcctatcg tatctctgaa aagcccaatg cattagcggt aatggaaggt
    11461 ttggaggaag ctatccagac cactaacgat tgtccctatc gtcgtacgtt tcactcagat
    11521 caaggttggg cgtatcaaat gaaagcttat agaaacaaat tgaaggagta caaaatcttt
    11581 caaagcatgt ctcggaaagg gaactgctta gataattcac ttatggagaa tttttttggc
    11641 ttattaaaac aagagatttt tcacgggaaa gtttataact gtttcgagga attaaaatca
    11701 gccatcgata gctatatcta ttactacaat aatgaacgga taaaacagaa attaaactgg
    11761 caaagtctcg tccaatttcg taaaacaacc gtaacggttg tgtaaaaata tcattttaga
    11821 gcataaaaaa aaggagtgga tttctccact ccaaaaaagt ctaacttttg ggggtcacta
    11881 cagtgtcaca gtcctttttt tagtaaaagg atacatttct tccttgtata ttccatgcgt
    11941 gttcgtgaga aagcatatat aatttaatta acagatagtt agggggagaa aaaatgattt
    12001 tttcaattac aatgaaactg gtgttcgggt tgatcggttt attattagtg gtacgtttgc
    12061 ttgggaagaa atccatgtca gaaatcacac cgtttgattt agtctatacg cttgttttag
    12121 gtgggatctt agaagaatca acctatgatg acaatgtcca tgtagggcat gtcctatttg
    12181 ccattgcgct ttgggcagta atgatttatg gtatcgaacg tatcgtccaa aaaaatgaaa
    12241 aggtcaatcg ttgggtcaaa ggcgagccat ctgttctaat caaagacggc gtcatcaaca
    12301 tgacagagtt gacaaataac catatcgaga tggagcagtt gcgagcaatc ttaagacagc
    12361 aagaatgctt ttttcttgaa aatgccaaac atgttatcct agagaatgcc ggtcaaatga
    12421 gtgtattgaa aaaatcagat gaagacaaag ctttatcgat cctgcttgta gatgaagggc
    12481 aaattcagca taaagtgctt cagtccaatc aattgactga agcatggctt atggagaact
    12541 tgaagaaaga aggctatgca gatgtaaaac agctgattta tgttgaatgg tcagaagaaa
    12601 aagggctcta catactaaat aaagaagacg tcatgcatga atcttaccga atagatggat
    12661 agatagacag aggttgtgaa atcagcgcct tggcttgtta ctcaaaacta agcgactttt
    12721 tcacaacctc tgatatctaa tatacgttgt tccagaagtt gcttcttcga aaaataagcc
    12781 aaaattccac aaaaatttaa gaagcaattt tcgaaaaatc ctttcttatt ttttgaagtt
    12841 gatcacttct gtcacaacct ctcatgtcac aaaccatttt tttgtttcct ttaactcaat
    12901 ataccgtacg tcttcccaca atatcggctg atggtcaatc ataaagtaat cttcataggc
    12961 ttcaccaatg aagtgccctt cgacctcatc caagagtctt ccaaattcat cttttaaatt
    13021 gagctgaatg attactgctt tacgagataa aaaggcttct gtcagtatac tatcgatttc
    13081 ttgcaatgtc atttgttgtt taggaagagg ctgtttgcgt gcttcttctt catttttttg
    13141 ataccgttga ctagttcttc catcgcaaaa gcagtccccc attttaatcc aaatggccga
    13201 tctcgatact cgttatagtc aatgaatttt tttgctgtcc gtctcatctt ttccaccttc
    13261 taatccactc ataccacctg catgcccgcc aacgagagaa ctacgtgcga tggcgcgtcc
    13321 tccttgcaag agactatgcg catggatgat tgattgaaag ccgtattttt ttctaatcgt
    13381 atcgacaaca aaatcaactt tcgcttcatt tatctgcttt tcaggatctt caaaaaggtt
    13441 aagctgtaaa cctgtcgcgt atacgaggtc ggaacagttc acgcctacac gccgaatatc
    13501 ctggtagtgg taatgctggt ggaaaatggt caataaagca tctgcaagat ctctgctagc
    13561 atttgtcgct tcgattttca tttgctggtg aaacccggtc ttgccatttg gatcaagata
    13621 gcctaaagag tatcctaccc ataagctgac tacttgtgtt tttgctgcag atcgtctcaa
    13681 gcgagtgccg acttggtcgg ccatttcttt gataacgatc tcgatttctt ctcgtcttgt
    13741 ataatctcta tctaggattt gactatttcc gatacttttt gattttggtg tatattcttg
    13801 tcccaaaaaa cttcgatcga tgccccaagc atgggcataa agctgagtac cgatgacccc
    13861 tagcttgctt ttcaagtgat aataatccgc gtgagctaaa tcataaacag aatgtatacc
    13921 catttgtttc agacgttttg ctgttcgttt gccgattccc caaaagtcag tgatatctga
    13981 gattttccat aacttttctg gtacatcttc ataacgccat tcagcaaaca tttgctttcc
    14041 gtcttttgag ccattgtcta aggctagttt agcaagtagc gggttatcgc caattcctat
    14101 agtcgtgtag attccggtct gctcaaacac atctttatgg atcagtttag ccatgtcgta
    14161 cgcatttgtt ttaccaaaga gtgccatgct atttgttaca tcaaggaagc tttcatcgac
    14221 ggaatagaca tgatggttcg cttcgtctgc atatttttta tagatagaat tgatttctct
    14281 gttcttttcc atataatagg ccatccttgg ggcaacaata tataaatcac ttggataggg
    14341 gaagggaaga tctctggcac ggctgatatt ggtgatgccg taagcttttt ttgctgcagg
    14401 actggaagcc aagattaatc cgctaccccg tgtttctgtc gaatcggacg gataggacat
    14461 gactactaat ttagttgtaa ggggatcaag atccttttca acgcactcta cggaggcgta
    14521 aaaagatttg cagtcaatac ataaaatatc tcttgaaggt tctttttcat aaacaaatac
    14581 cggattttga ttcgcccaca taacaatcac ctcttcaaca caattagaac acacgttctc
    14641 tttccgtgtc aataaaaaac agcaattttc aaatacgagc aaaaggctca catttggaaa
    14701 ttgctgagag gttattttta tctatttgtt tttagctgag atcgttgatt tttttatcaa
    14761 acatgaacag cttctggatc gatctttgtt gtcccatttc caatgtgtgc aacataaaat
    14821 tccggagcat agccgacaac tttcatgtat tcatcataga cattgttttt gaaggcaggt
    14881 atattttctt ctttgacaag agcaatcgca cagccaccaa atccagctcc tgtcattcgt
    14941 gcacctaaga caccttcttg tttttgagca gcatctacta gtgtatctaa ttcgatccca
    15001 gttacttcat aatcatagcg aagagagtgg tgagaatcat ttaacagttg accaaatgct
    15061 tctaagttcc cagcttctaa ttcagctttt gccttcaaag tccgctgatt ttcagaaacg
    15121 gcatggcgag ctctgcgtat caatgtatca tcgccgattt ggtcaatagt tgattcaaat
    15181 tcttcttcat ctaagtctcc taaagaagag ataggcaaag ttttttgcaa acgtgccaag
    15241 gcttcttcgc attcgctacg gcgttcattg tattttgaat ctgccaattc ccgccgctta
    15301 ttcgtattca tgatgacgat tgcataacca tctagtttca ctggaaccat ttcgtatttc
    15361 agcgtatttg tatctaataa gattgcatga tctagctcac cgaagccgat ggcaaactga
    15421 tccatgatac ctgaattgac accgatgaat tcattttcta cttttttacc tgtttggact
    15481 agttccaagc gatctgtttt taaatcaaac agatcttcta atacaacacc tactaataat
    15541 tctaatgaag cgctactgga aagtcctgca ccattaggga ttgttccttc cactaataac
    15601 tcaaacccat gatcgattga gtgtccagct tcttttaatt taaggaccat tccttttacg
    15661 taatttgccc agtttgcttt tttatcatat tctagctcat ctaaagtaaa actaatgaca
    15721 ccgatatcgt caaagtttgt ggaataaact aatattttat tatcgtctcg aggtgctgct
    15781 acaccttgcg ttccataagt gatggaagcg gggaagacat ggcctccatt gtaatctgtg
    15841 tgttcaccaa tcaaattgat gcgtcctggt gagaagtatt gtgtagtttt tttaggtccg
    15901 aatgtttcgg caaattttgt gtttaagctt cttttgattt ccatgatgag ttattccttt
    15961 cttgttttga ttttgaattc tgttactgtt tcaaatactg atccggcttt taaatgaatc
    16021 gaaccaaatg ctgagaagcg ctctgcacct ggagctgttt gagtctcaaa tgtgatgcca
    16081 ccgtgatgtg ccagttttcg gttgcgcatt tctggtgttt ctgtgccgaa atttgcagtg
    16141 aaaataacca cactagaagc atctgtagca atatctactt ggattctttt atcggggcta
    16201 gtcaagcgag cggcttcttt acccaagccg gtttctttta agaaaaatgg atgatcgatt
    16261 ccgtcaacaa gatttttttg atccaaatca ctagcaaaaa cggacgctaa ttttttaggt
    16321 atttgaaaat cgaaagcact tcctgcagca ttttctttta cccctatagg aatactatct
    16381 gttcgcaaag gagcataagc ttcgctattg agccaaagct catgttgatc gattgactga
    16441 gaagcatctc ccgtcaagtt gaagtaaaca tgatttgtcg gattgaataa agtatcttgg
    16501 tcactggtgc cgcgagaggt cactcgccag atattatctt ttgtcaaagt atagcggatt
    16561 tccacatcca ttgtacctgg aaaaccatgt tctccgtcgg gacttgtagt agtaaaaatg
    16621 acagaagctt cattttcgcc attcaaaatg acataagacc atttttttaa ttcaaatcca
    16681 ggagcaccac catgcagatt atgtccagtt tttggatctg tggctaattg gtaagttttt
    16741 ccattgagag aaaagcggcc attttcgatt ctccctgcgg tgcgaccgat agaagcaccg
    16801 atatacggat ctttttcgat atattcttca gcggtgtcaa atcccaaaac taattctcga
    16861 tcatttgatt ttaaactgac gattctagca cctaaatcag agatagaaag tgctagcttg
    16921 tttttgttct ccaaggttat caagtgataa ccttggccaa aatctttttc tgtaatcttc
    16981 attttcaaga tcctttctta atgggaagga actactgcat cgtgaagtta atcttccatt
    17041 gtatcagctg gagccaattt gttttccaat ttctcaacga tttgtgcatg ttttttctct
    17101 gtcaatgtaa tcttccaagc aaaaataata gcggagaaaa tcatcaaagc agcaggagca
    17161 tagaatgcat aagctttgaa agtcgttacg ccatgagcag tgatatcagc agcagtagca
    17221 ttaccagtca tcccagaagc gatagcgaca aaccctacga tcccgttaga caaagcacca
    17281 gcgattttat ctagtaaagg gcgaattgat agagtaacag cttcgttacg gataccattt
    17341 ttccattgac catattcaac agagtctgtg atcgtcatca aagcggataa gaaaatcaat
    17401 tgttgaggga agtagaacaa tactagcccg atgataacga ttggcaaact tgaacctgcg
    17461 atcgtaaaga aaaagtaaga agaaatcatc attgcgatcc ctccaaggta aacgtagcga
    17521 cgagagatga cgcgtgtaag cattgggaat agtggaacag ctactaaacc aagtaaggca
    17581 gcaactagac caacgatcca aaattcagtt ggttggccaa gtacatattt gaagaagtag
    17641 aataatacgg cagttgttgc tgcattggca atcgcataag cgatataagc aagggcaagc
    17701 cacatcaact gatcattttg agcaactgct ttgaacacat ctttgatccc agctttttca
    17761 ttttgctgac ggatcgcagt atcgacttct tttgtgccca aggcagtgat aatcgcagta
    17821 ccgcctgaaa taacagcagc aatcacaccg aaccagaacc agcctgaagc gccttcaata
    17881 tgtttatgag taacaagata ggtaaagaaa gtaacgatag ggacaacgac tagagtagta
    17941 ccatttgcac ctagagaaga gccgaaacga gcaaacgtag caaatttttc acgttccttt
    18001 gtttccgtac tcaaagctgg aatcattgac caaaaagcga tatccttaaa tgaataaaaa
    18061 ctatccaata agatgaaaac gattgcaaat aatatgataa ataaagtcgt attatgagta
    18121 gctaagccaa agaaattggt aaagattgct accaagagta cggaactaac aacgccgccg
    18181 ataactaacc atggtttgaa tttaccgtat tttgtacgag tattatcgat gatgccacca
    18241 atcaaaggat caaaaataat ttcaactaag cggataccta caactaagct tgtaactgta
    18301 gcaatcattt ttgcattcgt tgccttatct gttcctgcga acatctggct tgtcacgaat
    18361 accataaaat aagtgcttaa tgtaacatag aatgtgtcgt gtccaaaagc gcctaaagcg
    18421 taggacacac gttggatcat tttgcttttc ataatctgta ttcctctcct gttcaatata
    18481 taatactaag taaaccggtt acaacgaata acaaagatct tttaatgact taaacttaac
    18541 aagaaatcgc ttccttttta atatatttct gtgactttta tttaactttt tgttgcatta
    18601 tactaaagga gtaaggaggg atagacgtgg agcaatcgat ttttttaaaa aaggaacatc
    18661 aacaagtaga aaaagaattg tattttgatt tttgtggttt ctctaaaacg atgccgtttc
    18721 attcttttgg tcctgctatt cgcaatgatt acatcctgca tgtcgtgatg gaaggcaaag
    18781 gatcttatca tgtgaaaaat caacaattcc aattgaaaaa aggtgatctg ttcttgattc
    18841 gtccaggaga ctcgacgttt tacttatcag atggagaaga tccgtggatg tattgctggc
    18901 tttcatttgg tgggagtgct gcagaagaaa tcattcggca gtcgcttttc agagaagacc
    18961 aatacacgat ggtctcttct gacatacaaa aatatatcga cattatttta gaatgcatga
    19021 aagatacgga agaaaccttg gaaaatgaat tacagttaac agcattaacc taccgttttt
    19081 tatcagtcct tttaaaagat ggcggaaagg tcagcggtgg tgtacaaaag gttcattcct
    19141 ctttagctgt tgaaacagtc aaatacatcg aggcacatta tgctgaaaaa ttgtctgttg
    19201 aggaaatcgc ccagcattta gcggtcaatc ggagccattt gtcgcgagta ttcaaaaatc
    19261 atatgggtac cagtatcaag gaatatttga tcggggtacg gatcaatcga ggggcgtttc
    19321 ttctttctct gacggatgaa tccgtggaaa gtattgccta tcaagtaggg ttcaatagtc
    19381 tagtcgtatt tagccgaatg ttcaaaaaga gtaccggaga aacggctacg aactatcgta
    19441 aacgtatgaa aaacgaaaat tttaagaata ttgctttgga taccttgaaa aaacagttgg
    19501 aaaaacaaac cgcagtttct tggacaacct gattagtctg gttaaaatga caagcatcgg
    19561 caaaagaaac ttgtggcggt tatgctacac tgtaaagcga agactaaaaa ggaggaaaag
    19621 ggatgcgtac acttatcatc attagccatc caacgatcga agaatcttta aatcagcaat
    19681 ttttcaaaga agcttctgct ggtttaccag ctacttggca tcatttggaa tcaagttatc
    19741 cagacggaaa aatagatata gagactgaac tacagctctt ggcccagcat gatcggatca
    19801 ttttccagtt tccgttttac tggtatagtt ctcctgctca tttaaaaatt tggcaagata
    19861 atgtcttgga acgagcagca catgtattaa aagggaaaga actaggaatc gtattgacaa
    19921 caggtgtggc ggaaaaagaa taccaagctg gaggaaaaga agaatatacg atttccgaat
    19981 ttttgaggcc gtatcagcga atcgcaaata aattccatat gacctatctt ccgccattcg
    20041 ttttagcaca atttatgtat ttatcccagg aaaaacgttg ggagaagcta attgcttatc
    20101 agcagtatct ctcattggaa ggcaaaccat cgctgactca gcggattgac tggtttattc
    20161 aaagagtaca ggaaaatcag aaaatgcagg aagaagaatc tgaaaaacaa gcatatatcg
    20221 tagaagcact gactgaggca aaagagcaga tcgaagactt gacctttaca ctgcaagaaa
    20281 tgaaaggaac ttcattatga caaatgattg gctgatccaa caattagatg aattaagcga
    20341 aaaatcagaa tatcaagacc gtgcgctctt atatgaaata aagaaactga tggctgagat
    20401 agacgaacga acaatgcagc tgcaaggaga aatcgacgga caatcttgga atcatctcaa
    20461 ctggtgaaat tttcttataa ataacaaaga taaaaatgtg cacagcgaca attatttgct
    20521 acaataagaa tatgaagcgt ttgataacgc tattatacat aaaaattggt ggtgaaaaaa
    20581 atgtatgatg aaaatgagtg gggagaaaat gttcggatga cccgaagaag acagcaaaaa
    20641 caggcagctc gtaagcggat gatcatgatt ggggcagcag ttgccgtgtt tttgatcata
    20701 gcaggaggag ttgcgggtct atattttcat ggaaaaaatt cgtcagaagc gaaaagtacg
    20761 accataacgg aaacaacgac aaagaaaaca gagacgaaac aaacaagtga gtcatcaact
    20821 aaaacaaaag agacacaaac ttctgaagaa aaaacagcaa atggtttgcc agagcaactt
    20881 caagcaatcg taaatgatta ttcgaaaaag atcgaagaaa agacgcctgc attgattgaa
    20941 gaataccaga cagaaattca gggaaatcaa gagggaatag ctggtttatc agcagttgct
    21001 aaccaaaaag cacgtgagct tcaagcaatc tccgacgaag ggatcagaaa attacgtgca
    21061 gcgtatcagg cagcagaaaa taaagacggt gtagacttag atactttgat caatcaatta
    21121 tcggctaatt atacgaatca tgtcgctaag atttccgata tttatttgca gactagcgcc
    21181 tctcttcaag cagagtcaac aagcacacaa gacacaacat cttctgattc tgagacagca
    21241 gagtcggtag aagcttctga aatgcaagct cgaacaacac aagacacaac agattcctct
    21301 gaaactaata ccaacagcgc gacttcacag gctgcgacaa ccgtagtttt ggaaggagaa
    21361 ggtccaaatc aaatagccga acgaactggc gtaccagtag aaacgatctt gtcgctgaat
    21421 gggatgagca tggagaatta tttcctgact cccggtgaga cgctaaaatt gaattgataa
    21481 aagcaataaa aaagaacgaa gcttccacta aggtgaagct tcgttctttt tttattttcc
    21541 ggttcttttt tcgcgtacaa agtaatggat cgtcatgtaa agcaagaaaa cgagtccaat
    21601 aatcaatgag attctcgttt caggattgat aaacatgaaa atcaatacga tagctaaaaa
    21661 agcaagtgtc acataattcg tataaggaga tagtggcatt ttaaaaggat gatcatgcat
    21721 tttatctgcg tgcttctttt tgaagcgaat ctcgctgatc aaaatcacta cccaaggaac
    21781 cataccaggc aaaatacttg agctgtatac gaaaacgaaa atattttctg actggttgat
    21841 gaacattggt agaagataat ttaagatcag accaattaag attccacctg agattgctac
    21901 gacaggataa aaaggcgcac cgtgacgaga tagtttcaga aattttttag acatctttcc
    21961 atttatggca agtgtataga ccatacgact tgcgctgaaa atccctgaat tgcaacctga
    22021 taatgccgct gtaatcacga caaaattaat cagtcctgca gcaaatgtaa tgccgacttt
    22081 agaaaaagtt tgtacaaaag gtgaaccaat ctcactcaat tgattccacg gatagatact
    22141 cacaatgata aaaatagcac caatataaaa aatcaagatt cgaccgatag tggactggac
    22201 agcttcaatg atcgtctttt taggattctc tgcttcacca gcagtcattc cgatcagttc
    22261 gattccttga taggaagcaa caacgatcga taatgcaaag aaaaatcctt tgacgcctcc
    22321 agtgaagaat ccaccatttt tccaaagatt tgaaatccct actgcctgac catgattacc
    22381 aaatccaaat aagattaccc caagacctgc aatgatcatc aaaatgatcg ttatgacttt
    22441 gatgatcgaa aaccaaaatt ctaattcgcc aaacgccttg acagaaataa gatttgccag
    22501 cataagaaac aagacgacaa tgattccggg aataattgcc ggcaaatcag gccaccaata
    22561 attcatataa gatcctacag caatcacttc acttattcct acgacaatat attgaaaaat
    22621 attactccaa gcggtcaagt agccagccac aggatggatg tactcgctcg cgtaatttgc
    22681 aaaagaacca gtcgaaggat caacgtaaag catttcgcct aaagcacgca taatcaaata
    22741 caaaaagaat cctgcaatcg cataggctcc catcactgag ggccctgtcc aacgtatggt
    22801 tgaggaggca cccataaaca gaccgacacc gatcgttcca cctagtgcga tcatttggat
    22861 atgacgggca ctaagacttc gagttaactt ttcttcttcc atacaaaaac tcctttcgtt
    22921 gttgagtcaa caagaaaaaa taatcaaacg tttcaacaca tgtttttcat tattaggtta
    22981 gccttattga actcttgatg tgattctaca gaaaattttc ataaaatgcg aggaaaattc
    23041 gaaaaaaact ttttcatcat tctacttgaa aatgctttcc attttagtta aactatattt
    23101 aaataaaaag ttaggtaaac ctaaaaagga gtgtaagatt tggaacgtac ggccattgta
    23161 attcaagacc tttcagccgc ttatcaaggg aaaactgttt tgaaaactat caacttgacg
    23221 ataaatccac aaaaaattac cggtattatc ggaccaaacg gtgcaggaaa atcaacattt
    23281 atgaaatctt tgctagagtt gataccagct actactggta aagtcacctt ttctggacag
    23341 tcggtaaact cagtccgtaa aaaaatagcc tacgtggaac agcgaagtga attggatctt
    23401 tcctttccaa ttgatgtatt aggcgttgta cttttaggaa catatccatc tttacgaatt
    23461 ggacaaagac ctgggaaaaa agaaaaagaa cgtgcaagac aagctttgaa aaaagtaggg
    23521 atggaagaat atgccaaaag acagatcagc gaactatcgg gtggacagct tcagagagtt
    23581 tttattgcaa gagctctagc ccaaggagca gaatggatct ttttagatga accattcgta
    23641 gggattgatg cgttaagtga acgaaagatc tttgacatct tgcaggaatt gaagaattca
    23701 ggaaaaacga ttttgatcgt ccatcatgat cttcataaag tagaagaata tttcgatgag
    23761 gttattcttt taaataaaca gctgatcgct tccggtccag tacaagaagt ctttacatcg
    23821 aagaaccttc aattggccta tggtgagatc atcagacatt tagtaaaagg aggagagaaa
    23881 aaatgatcca atcctttatt gatggattaa tgaattatca attcctgcaa tacgctttgg
    23941 tcacttccat gcttgtagga cttgcttctg gagtcatcgg ttcttttatt attttacgag
    24001 ggatgtcttt gatgggagac gctatctctc acgcagttct tccaggtgta gccatttctt
    24061 atatgtttgg ttttagttac atctttggag ccactgcttt tgggatgcta acagcagcag
    24121 tgattggttt cgtgactcag cggagccgat tgaaaaatga tacagcgatt ggaattgtct
    24181 ttagctcctt ttttgcatta gggattatct tgatttcata tgcccagagt gcgacagatt
    24241 tgtatcatat tttatttggg aatgtcttag ctgttaggga aagcgatttg ttgctgacag
    24301 cactcgttag tggaatcgtc ttgatatttg tctttttctt ttataaagaa ttgaagatca
    24361 catcattcga tccgacaatg gcaaaagctt atgggctgaa cacatcgctg atccattatc
    24421 ttttgatgtt ctttttgaca ttagtggctg tagtcagttt acagacagta ggaacaatct
    24481 tggtgattgc catgttgatc acaccagccg ccacggctta cttgctaacg aaccatttac
    24541 tgaaaatgat cattacagct gcaggaatcg gtatgctaag tgcagttgtc ggtgtgtttt
    24601 tcagttatag ttacaattgg ccatcaggag ctacgatcgt gttagcatgt accgcatttt
    24661 ttatccttgc ttttttattt tctccaacaa aaggaatttt atttcagaaa ggaagcagat
    24721 agatgaaaac aaaaaaatca ctctttttaa tcttggcagt tagtttcttg gtattagccg
    24781 gttgcggaaa gcaggcttct gaccaagcgg atgaaggaag taaagaaaag ttgtcagtcg
    24841 tggctaccaa ttcgatcttg gcggacatgg caaaagaggt cggaacagat caaatagata
    24901 tccacagtat cgtaccagtc ggaacagatc cgcatgaata tgaagtacta ccagaagaca
    24961 tcaaaaaggc aagcgatgca gatgttattt tatacaacgg tttgaatctt gaaacaggta
    25021 acagctggtt cgataacttg atggaaacgg ctaaaaaaga agaaggaaaa gattattttg
    25081 cagttagcaa aaatgtagaa cctctttatt taactagcgg tgaagaacat acaaaagcag
    25141 atccccatgc ttggctagac ctatctaacg gaataaaata tgtggaggaa atcgcacgta
    25201 tattctctga aaaagatgca gaaaatgcga cactctataa aaaaaatgca gaagcatatg
    25261 tggaaaaact aaaagaatta gatacacaag cgaaggaaag ttttgcttct atcgaagaga
    25321 acaaaaaatt attagtaaca agtgaagggg cattcaagta tttttcacga gcatatgatt
    25381 tgccagcagc ttatatatgg gagatcaata cagaaagtca aggtacgcct gatcaaatga
    25441 aagcgatcat tgatcagata agagcaaaag aagtaccggt tttattcgtg gaaaccagtg
    25501 tagactcaag aagcatggaa cgggtagcaa aagaaacggg tttgaaaatc tatgataaac
    25561 tgttcactga ttccatagca aaagaaggag aacaaggaga ttcttactat cagatgatga
    25621 aatggaatat tgaaacgatc catgaaggtt tgtcacaaac aaaagaaagc tgaaaagtgc
    25681 aaggtctagc ttgaggagca gaccttgcat tttttataag agatatgaaa aaaaattatg
    25741 ctgtttttcg tcgtacagat accttttttg aagaaattcc gttatagtta gagtaaggaa
    25801 ggaaggtgtg gctatgaatt ttacacgtaa agataaatat acgattaaag aagaagtctt
    25861 tcgattgatc agccagcagg aaaataccga caaggatcaa gaccttcaat ttcttgaagc
    25921 agaattagct aaattattca tagcaaatca aaatgagaca acagaaccag atgaagagct
    25981 gtacataggc aaatggttgt ttgatgaatt tcagccttta gatgaaaata ccggtatcgg
    26041 ttattttgtg ttcctttctc cagtcggctt cacacctggc gtattagtta aacaaaacga
    26101 cgaaataacc gtcaaagcat ggcctgcatt attttcagtc atcaaagcag aaggagtaat
    26161 caaaaagatc cagtcggcct attataaaag tcaatttatt gccaacagca aattaatgaa
    26221 tatgttttat aatcgtctag tagaaattgc taggcctggg aaaatcccgc aaacattcac
    26281 gtacacacag caaaaaaatc tctatgaaga agtattgtat tcccgcgatc tgtctgagtt
    26341 ttcgccatgg gaagaggtaa ggctccatga actgttaagt acaaaaccag tttcattgaa
    26401 tttttttaat gcattagata acaaaagaaa attaatttca acaaattatt gggaagtatt
    26461 tgctgcaaga aaaaaatgga tggaagaaaa gaagattgcc gatcagcaga tccagtttgt
    26521 ccagctgaaa aaagaaaaac tatattatgg gttatacagc cgttaacagc aagcgaggaa
    26581 gaccatgaca ttaaaagaag aaatcataca agaaagcaaa cgattaggaa ttgacaaaat
    26641 cgggttcacc acggcggaac cttttgattc attaaaagaa tcgttggaag aacaaaaagc
    26701 agctggccat acttctggct ttgaacatcc aaatatcgat gagcgtcttt atccagaaat
    26761 gacatttgat cagccgaaat cgatcatttc cattgcactt gcgtatccta ctaggatcca
    26821 tgaagaagtg cctagggatg aaaaacgcgg gcagtttgct cgtgcttctt gggggatcga
    26881 ttaccatgat atactcagag accgtttgaa ccgattgatc gaattcatcc aaacaagagc
    26941 agaaaaatgg caaaaagaag aggaatggcg tctagctccg caagtagata ctggagaact
    27001 gatcgatgtc gctgtagcac agcgagctgg tttaggattt attggacgaa acggattgtt
    27061 gatcactgaa gagttcggtt catttgtcta tcttggtgag atcgttacta atattgtatt
    27121 tgagcctgat aaaccaggag aatttggttg cggtgactgt atgcgctgcg tgactgcttg
    27181 tccgacaaaa gcattgcttg gtgacggaag aatgaatgcc cagcgatgtt tgtcttatca
    27241 aactcagaca aaagggatga tgccggaaga atatcgtaaa aaaatgcgaa atgtcattta
    27301 tggatgtgat atctgccagt tagtctgccc ttacaatcaa ggaaaagatt ttcattttca
    27361 tgaagagatg gaaccaaaag tagacgaaat atatccaaaa ttaaagccaa tgctttcgat
    27421 gtctaataaa gacttcaaac agcaatttgg gcatttagct ggttcatggc gtgggaagaa
    27481 acctttgcag agaaacgcat tgattgcttt agctaatcta ggtgatcgaa cggcattgcc
    27541 tgagatttca gaatgtttaa cagatgtccg ccctgtcatc agaggaacag cagcttgggc
    27601 tattggacgt ctaggaacaa aagaaccgga aaaatggctt gatgtattag atgaaatatt
    27661 agctaaagaa acagaagaag tcgttataaa cgaattggaa catgcccaga aattattgcg
    27721 gagaaagtta aaataaaaaa atagttaaca ttattcaaag tttttgtcca gtaattgttg
    27781 taacaattac tggttttttt gtatttttta cattttttta agagaatttg ataaaaatca
    27841 aaaatttttt tgcttttttt tatggattat tgcagtgaat gttacagaat ctgcgttttt
    27901 cattcaaaaa taaaaaaaca tag
//
LOCUS       DACDHL010000029        27633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDHL010000029 DACDHL010000000
VERSION     DACDHL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.029"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IV228_001292"
     CDS             complement(287..649)
                     /locus_tag="IV228_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717220.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IV228_001293"
     CDS             complement(723..1103)
                     /locus_tag="IV228_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8717221.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IV228_001294"
     CDS             1185..2360
                     /locus_tag="IV228_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8717222.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IV228_001295"
     CDS             complement(2437..3021)
                     /locus_tag="IV228_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8717223.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IV228_001296"
     CDS             3222..4220
                     /locus_tag="IV228_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8717224.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IV228_001297"
     CDS             4332..4841
                     /locus_tag="IV228_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8717225.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IV228_001298"
     CDS             complement(4871..5530)
                     /locus_tag="IV228_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8717226.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IV228_001299"
     CDS             complement(5532..6296)
                     /locus_tag="IV228_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8717227.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IV228_001300"
     CDS             complement(6304..6981)
                     /locus_tag="IV228_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8717228.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IV228_001301"
     CDS             complement(7018..8403)
                     /locus_tag="IV228_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8717229.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IV228_001302"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV228_001302"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IV228_001303"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IV228_001303"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8717230.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IV228_001304"
     CDS             9342..10199
                     /locus_tag="IV228_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8717231.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IV228_001305"
     CDS             complement(10280..10615)
                     /locus_tag="IV228_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717232.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IV228_001306"
     CDS             complement(10682..12052)
                     /locus_tag="IV228_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8717233.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IV228_001307"
     CDS             complement(12130..12666)
                     /locus_tag="IV228_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8717234.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IV228_001308"
     CDS             12975..14456
                     /locus_tag="IV228_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8717235.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="IV228_001309"
     CDS             complement(14559..15266)
                     /locus_tag="IV228_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8717236.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="IV228_001310"
     CDS             complement(15345..15683)
                     /locus_tag="IV228_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8717237.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="IV228_001311"
     CDS             complement(15794..16756)
                     /locus_tag="IV228_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8717238.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="IV228_001312"
     CDS             complement(16771..18102)
                     /locus_tag="IV228_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8717239.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="IV228_001313"
     CDS             complement(18110..18790)
                     /locus_tag="IV228_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8717240.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="IV228_001314"
     CDS             complement(19009..19488)
                     /locus_tag="IV228_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8717241.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19615..21294)
                     /locus_tag="IV228_001315"
     CDS             complement(19615..21294)
                     /locus_tag="IV228_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8717242.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21842..23344
                     /locus_tag="IV228_001316"
     CDS             21842..23344
                     /locus_tag="IV228_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717243.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23357..24019
                     /locus_tag="IV228_001317"
     CDS             23357..24019
                     /locus_tag="IV228_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717244.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24120..24323)
                     /locus_tag="IV228_001318"
     CDS             complement(24120..24323)
                     /locus_tag="IV228_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8717245.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IV228_001319"
     CDS             complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IV228_001319"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8717246.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IV228_001320"
     CDS             complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IV228_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8717247.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8229 a         5768 c         4673 g         8963 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ctattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taataaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt
    19681 ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc
    19741 aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt
    19801 catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga
    19861 aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga
    19921 caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat
    19981 cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag
    20041 cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa
    20101 atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt
    20161 catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt
    20221 ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac
    20281 gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat
    20341 cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat
    20401 tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt
    20461 aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat
    20521 tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa
    20581 tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca
    20641 atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg
    20701 attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc
    20761 caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc
    20821 cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt
    20881 atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc
    20941 taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc
    21001 taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg
    21061 aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat
    21121 gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc
    21181 aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat
    21241 gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt
    21301 cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat
    21361 gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag
    21421 gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt tggatatact
    21481 tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca
    21541 gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa
    21601 caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc
    21661 atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac
    21721 tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt
    21781 taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa
    21841 tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt
    21901 attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag
    21961 cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag
    22021 tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac
    22081 tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga
    22141 aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat
    22201 ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac
    22261 tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc
    22321 agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac
    22381 agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt
    22441 cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac
    22501 gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag
    22561 cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta
    22621 cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc
    22681 tgctatttac actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa
    22741 gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt
    22801 ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc
    22861 cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt
    22921 aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga
    22981 agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc
    23041 gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca
    23101 agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg
    23161 tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat
    23221 caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt
    23281 ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa
    23341 ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca
    23401 agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt
    23461 aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc
    23521 aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta
    23581 tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc
    23641 tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca
    23701 agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt
    23761 gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga
    23821 acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga
    23881 tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac
    23941 tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt
    24001 tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct
    24061 gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt
    24121 tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct
    24181 ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg
    24241 tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta
    24301 gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct
    24361 tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag
    24421 ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt
    24481 ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga
    24541 tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac
    24601 gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca
    24661 cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat
    24721 gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat
    24781 ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat
    24841 ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt
    24901 tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa
    24961 attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt
    25021 ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac
    25081 gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg
    25141 atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag
    25201 aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc
    25261 cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt
    25321 ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga
    25381 cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac
    25441 agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct acttccactc
    25501 cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta
    25561 catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac
    25621 atttttcctc tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat
    25681 aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg
    25741 tttggattac ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa
    25801 tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg
    25861 cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg
    25921 gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg
    25981 atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg
    26041 aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa
    26101 tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg
    26161 aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta
    26221 cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga
    26281 ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa
    26341 agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc
    26401 agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat
    26461 tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg
    26521 aactttctgg ttttttacgg attttttctg catatatcat catataacca gcggctaatg
    26581 cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag
    26641 caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt
    26701 gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa
    26761 atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac
    26821 cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac
    26881 tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac
    26941 ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata
    27001 ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata
    27061 ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt
    27121 aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc
    27181 cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg
    27241 gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat
    27301 aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt
    27361 attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa
    27421 agatgactac accaaaaata atgacatacg tatggggtgt cgataattta gtgaagtcgc
    27481 gtttcggttt tcctgaagaa tttgttttct tcaaatttat ttcttccttt cttttttcag
    27541 taattttgat gaataaaaaa tggataatca aatgaattat acagtaaaaa tgacatttcc
    27601 acatgaataa tttattagaa aatcactttt ttc
//
LOCUS       DACDHL010000030        27232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDHL010000030 DACDHL010000000
VERSION     DACDHL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.030"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(161..445)
                     /locus_tag="IV228_001321"
     CDS             complement(161..445)
                     /locus_tag="IV228_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717248.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IV228_001322"
     CDS             complement(463..1353)
                     /locus_tag="IV228_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8717249.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IV228_001323"
     CDS             complement(1373..1591)
                     /locus_tag="IV228_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717250.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="IV228_001324"
     CDS             complement(1561..3261)
                     /locus_tag="IV228_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8717251.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="IV228_001325"
     CDS             3528..4301
                     /locus_tag="IV228_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717252.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IV228_001326"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IV228_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8717253.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="IV228_001327"
     CDS             complement(5125..6096)
                     /locus_tag="IV228_001327"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8717254.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IV228_001328"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IV228_001328"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8717255.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IV228_001329"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IV228_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8717256.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="IV228_001330"
     CDS             complement(9051..9875)
                     /locus_tag="IV228_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8717257.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="IV228_001331"
     CDS             complement(9848..10528)
                     /locus_tag="IV228_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8717258.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="IV228_001332"
     CDS             complement(10551..11489)
                     /locus_tag="IV228_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8717259.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /locus_tag="IV228_001333"
     CDS             complement(11734..12585)
                     /locus_tag="IV228_001333"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8717260.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IV228_001334"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IV228_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8717261.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="IV228_001335"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="IV228_001335"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8717262.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IV228_001336"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IV228_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8717263.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IV228_001337"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IV228_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8717264.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="IV228_001338"
     CDS             complement(19463..20704)
                     /locus_tag="IV228_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8717265.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IV228_001339"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IV228_001339"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8717266.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22615)
                     /locus_tag="IV228_001340"
     CDS             complement(21731..22615)
                     /locus_tag="IV228_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8717267.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="IV228_001341"
     CDS             complement(23392..24678)
                     /locus_tag="IV228_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8717268.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="IV228_001342"
     CDS             complement(24671..25939)
                     /locus_tag="IV228_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8717269.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="IV228_001343"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="IV228_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8717270.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="IV228_001344"
     CDS             complement(26711..27157)
                     /locus_tag="IV228_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8717271.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7741 a         5873 c         4723 g         8895 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tc
//
LOCUS       DACDHL010000031        26894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDHL010000031 DACDHL010000000
VERSION     DACDHL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.031"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(161..559)
                     /locus_tag="IV228_001345"
     CDS             complement(161..559)
                     /locus_tag="IV228_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717272.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            775..1830
                     /locus_tag="IV228_001346"
     CDS             775..1830
                     /locus_tag="IV228_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP8717273.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            1850..2203
                     /locus_tag="IV228_001347"
     CDS             1850..2203
                     /locus_tag="IV228_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717274.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2385..2681)
                     /locus_tag="IV228_001348"
     CDS             complement(2385..2681)
                     /locus_tag="IV228_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717275.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2685..3503)
                     /locus_tag="IV228_001349"
     CDS             complement(2685..3503)
                     /locus_tag="IV228_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8717276.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            3729..3950
                     /locus_tag="IV228_001350"
     CDS             3729..3950
                     /locus_tag="IV228_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP8717277.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            3952..4218
                     /locus_tag="IV228_001351"
     CDS             3952..4218
                     /locus_tag="IV228_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8717278.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4331..4870
                     /locus_tag="IV228_001352"
     CDS             4331..4870
                     /locus_tag="IV228_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717279.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            5364..5648
                     /locus_tag="IV228_001353"
     CDS             5364..5648
                     /locus_tag="IV228_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717280.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            5922..6176
                     /locus_tag="IV228_001354"
     CDS             5922..6176
                     /locus_tag="IV228_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717281.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            6258..6443
                     /locus_tag="IV228_001355"
     CDS             6258..6443
                     /locus_tag="IV228_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8717282.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            6510..6905
                     /locus_tag="IV228_001356"
     CDS             6510..6905
                     /locus_tag="IV228_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8717283.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELFEK"
     gene            6895..7272
                     /locus_tag="IV228_001357"
     CDS             6895..7272
                     /locus_tag="IV228_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717284.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            7273..7669
                     /locus_tag="IV228_001358"
                     /pseudo
     CDS             7273..7669
                     /locus_tag="IV228_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7975..8373)
                     /locus_tag="IV228_001359"
     CDS             complement(7975..8373)
                     /locus_tag="IV228_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8717285.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(8564..9331)
                     /locus_tag="IV228_001360"
     CDS             complement(8564..9331)
                     /locus_tag="IV228_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8717286.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(9366..10589)
                     /locus_tag="IV228_001361"
     CDS             complement(9366..10589)
                     /locus_tag="IV228_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8717287.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            11570..12373
                     /locus_tag="IV228_001362"
     CDS             11570..12373
                     /locus_tag="IV228_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8717288.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            12853..13308
                     /locus_tag="IV228_001363"
     CDS             12853..13308
                     /locus_tag="IV228_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717289.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            13427..14485
                     /locus_tag="IV228_001364"
     CDS             13427..14485
                     /locus_tag="IV228_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8717290.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            14527..16476
                     /locus_tag="IV228_001365"
     CDS             14527..16476
                     /locus_tag="IV228_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8717291.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            16479..20120
                     /locus_tag="IV228_001366"
     CDS             16479..20120
                     /locus_tag="IV228_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717292.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            20520..20858
                     /locus_tag="IV228_001367"
     CDS             20520..20858
                     /locus_tag="IV228_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717293.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            20968..21189
                     /locus_tag="IV228_001368"
     CDS             20968..21189
                     /locus_tag="IV228_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717294.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            21209..23365
                     /locus_tag="IV228_001369"
     CDS             21209..23365
                     /locus_tag="IV228_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717295.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            23377..23610
                     /locus_tag="IV228_001370"
     CDS             23377..23610
                     /locus_tag="IV228_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8717296.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            23600..24466
                     /locus_tag="IV228_001371"
     CDS             23600..24466
                     /locus_tag="IV228_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717297.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            24481..26451
                     /locus_tag="IV228_001372"
     CDS             24481..26451
                     /locus_tag="IV228_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717298.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            26618..26845
                     /locus_tag="IV228_001373"
     CDS             26618..26845
                     /locus_tag="IV228_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717299.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
BASE COUNT         9632 a         4118 c         4922 g         8222 t
ORIGIN      
        1 ataattaacc aaccgccaga taaaaatgcg atcattgcta atacataagc aaagataaaa
       61 atatgttcga tcagcttaaa ccctgatgcg attaagcaga ccaagtaaaa tagaaattct
      121 cttacagata atggtaattt tctaactagc atactcataa ttaacacccc aaagtaatat
      181 tgtattttga acattttaca tccaatacag catcaatcaa ccattgccaa tcaaaaatac
      241 aaggcttcat tgaagtatgt ttttcagatg aaaagatata atataatttc ttcggaataa
      301 tattttcagt tgttgggatc aagctaaatc taataccgtc taactcatag ctaccagctt
      361 tctctccatt aaaaatttcc ttagcacttt cttctaaaga atcaaaatat ttttcgacag
      421 caatctttga agcttttact attgaagcat ttcccttttc gataaaatct gcttttgttt
      481 taccgacaat aagtaattct tgatcagtaa aatcttctaa aataatggta gtgtaattct
      541 tatttcttct caatcgcatc aggatcactc cttaatataa tttatgtttt tataatatca
      601 taataagata aatttgcaag catttattta caccttttta tttacacttt attataatta
      661 attagacttt tatgattgca tttttttctt ataaacagtt gcaactaata gtaaataggt
      721 gtaaactatt ttcgtaccga aataccataa acacaaacag aaagaggtca taccatgaaa
      781 gaaccattat tgttttccgt tgacttaggg aataaatcag taaaagaaat gagtacacac
      841 gtttctccac tctcacttcc ctctcgtatt ttgaacaaaa atatcattaa ttcaggacgt
      901 ttttctaata taggttcctc attaaaacga tcactaaacg tttctgaata tagcctagta
      961 aacactactg aaagctatta ctttgggaag gatatacacg agctaggaaa agatgactac
     1021 gtgatagaat cactaggttt tgggatcagc agatacaagc aggaaaccta ccaaaatatc
     1081 ctatctttct ctttagctaa cctagcacgt ttagaagatc aatctgatga cgttttaacc
     1141 gctaatctag ttttaggtct gccttcatcc gactataccg aaacaatcgt caaatacgtg
     1201 atgcaacaag ccacacgaca acattcagtt gaagttgacg gtaaagctta taatataaac
     1261 gtagataccg tttgggtact gccacagccg attggaacac tatataacct cctacttgct
     1321 gatgatggta gtgtacaaga tgaaacattg atcagtcaga agatcggttt tgttgatatt
     1381 ggcggtggca cgaaactttt tgatgttgtc caaaattttg aaattgacga aaacaatcgt
     1441 gaacaaaagg ataccggcat ttttacttta tataatcgta tcgtaaatat gcttgacctt
     1501 ccgatcagtc ctaatgcttt ccaacttgaa aagattatcc gagaaggcaa agaaaaaggc
     1561 gtatatctat ttaagccaag cggaaatgaa agttttgata tttcagaagt agttgaaaga
     1621 gaaataaaaa catacaccaa tatggttgtt gcagaagttt tagctacatt caaaaacttc
     1681 acaaccttag ataaagttgt ctttactggt ggtggagctt ctattatcga caaacaactt
     1741 gtaaaagata aaattccgca taccttgttt gttgaagaca gtgaattttc taatgtaaaa
     1801 ggcttttaca agtttggttt aactcaataa aacagcgaag gagatcaaca tgaataaggg
     1861 aaaatcagag cgttatgtag ttataatcaa tagagaaaag gttagcgagt tacctttagc
     1921 agagtttctt gaatccgaat atgaaacact aggcgttaaa tactctcaga cgatcaaaaa
     1981 agctttgaac ctatatcggc gtgtaaagaa caatgaagtt acaattattg aagcaggcaa
     2041 cgatagaaac gatagcaaag tagcagaagt aaagaataaa ccaactgatc ataaccctat
     2101 tcaacaagag catccttcaa cggttacatc cgatcattca gcaaagaaag aaaagccaat
     2161 gctgaaaggg ttcaatgcac caagtaacaa agacttactt taaaaataaa aaggcgaggt
     2221 tacaatacct agccttttta ttatgtacaa atataaacat aaacatatac gtatacatgt
     2281 atataaacat gtatacgtat atatccataa acacgtatat taaatttagt taagctatac
     2341 ctaaccgctt attccaataa aaaaagtgat cagctttaaa ctgatcactc accaaaaact
     2401 tcatcataca agttatctat catttcacca agtaaagctg aatctgatcg aattcctaac
     2461 ttttcgctga ttttagatag tttctttctt tgcgatggtt gaagcgtgaa ttgataattt
     2521 ttcttctttt cagaactatc actttttagt ttagctacca aagaacttgt tgtttcttta
     2581 ggtgtttgtt taggttcttc aactgcttca accacttcag gaacttcttc tacttcagct
     2641 tcagcgaatt ttttaaatct attttctttc acttcaatca ctccttattc ttgtgccgta
     2701 tcaattattt tgttgtatag cgtaaacgtg ctttcaaaga aatccttatt cttacgatag
     2761 atattcttat ccttagcgaa ctcagcaata ttcttacctt ctaagctagt tgcattaagc
     2821 aattccttct cttgtacata tccaagataa ttagataacg ttggtaccag ttctaggaac
     2881 tcacgacttg aattagtatt atgtttgatc ctgctaccga tcgtagcgta tttagtttcc
     2941 atcagtgttt caccagtctt aatatcaatc aactgttttg ctagtgcatc aatatcttta
     3001 ccaagcttca gcaaagcttc aaaaccatct ttagatggat cagaaatgcc tacgacaaga
     3061 tcagatacag cccaagcatt ttgagctagt agacctgcat cattatgagt atcaatcaga
     3121 attagatcat acttctcttt aagctgttca tagttaacaa ccatccatct aaacagtagg
     3181 gttctgttgt taggcatatc acgcatttgc atctcaactt gttttaaatc ttcacctgct
     3241 actaaaatat ccatgttctc atttacagct aaaggttcag ctgatccatg aagatataaa
     3301 tgattgattg tattctctct tttcgcttta ctgaaagcat caggaaatcg ttttgatagg
     3361 ttttgcgact tatcttcatc aatatacagc accttcatac ctaaattttc gacagcatat
     3421 ccacctagat tgtagattgt tgttgtttta cctgttccac ctttttcaat gtttccagta
     3481 atgattgaca acttcttttc catgattaaa cctcccttat taaccttttg taactccatt
     3541 atacactata aacacgttta tgtatatgtt tatatacata aacgtgttta tatttatatt
     3601 tgcttttata ttctacaaca taaacatata tatgtatatg tttatgttta tgtatatatt
     3661 catatatata actattgcat ttgtcataca atcgtagtac aatgtaattg aaaaggagtt
     3721 gataaactat gccagtaata agtattcgtg tatctaaaga agaaaaagat tttcttgaaa
     3781 aagttgctga attcaacgga gataacattt ctgaatttat gcgtagtaat tccctgcaaa
     3841 cagcagaaac gttagtagac tttgccacct acaaggaact aatgaaagag catgaggaaa
     3901 acgatcaaag tatttcccac gaagatatgc taaaagagtt aggcttataa gatgacttac
     3961 caagtaagat atgaacgtga agctcaaaaa gcgctaaaaa aaatggataa gtttcaggca
     4021 aaggttatcc taaactggat cgagaaaaat cttgtaggca ctgatgatcc aagaaaacat
     4081 ggtaaaggtc taacagctaa taaatcaggc tactggcgat atagggtagg tgcctaccgt
     4141 ataatagctg atattagcga gcatgaagtg acaattctta tattgtctat tggtcacaga
     4201 agtgaagttt ataagtaaca gacaagctga taaggcttgt ctgttttttt gttaacccaa
     4261 caaaatcact gtttacgatc ccactttacc taaaaaagaa caataccctc ttgttattat
     4321 acacactggt gtgtataata taagtgtaaa caagctaatt aactttcaaa aggagcgatt
     4381 ttttatggga tggtatggtg cttattcatc agcaaatgaa gtgatcgaag aaaagaaagg
     4441 gtacgccaaa aatggtttgg aaatcgtaga taccaaagta acgaagacct atggtattat
     4501 ccttttcaaa gatgcagaag gaacctacga tattgacagc ttcatctttc gagatgaaat
     4561 gtacaaacca atctacttct taaatgacag tcctgcttac tttaagaaaa ttccgaaaaa
     4621 atggttgaaa ttctttgtcg gtgatcaaga aaaaatggtt aagcaacaac tagatagaat
     4681 taaagaagaa aacaataaac caaaccttga tgacttatta gttgcaggta aaacttactc
     4741 aatctgggat ggatcgtatg tcgctgttta tcagtataaa ctcaaacgta gctttatttt
     4801 tagactacaa gatggatcac tcactcggtt cactcgacta tcagcaggag atgttaagga
     4861 agtagcctaa aacttaaata cataaacata aacgtgtata cgtttatgtt tatgtttatg
     4921 tatatgtata tgaatataaa cgtattaggt acgaacgttt ttaaatgctt ttctgggctt
     4981 tgattttagg gtagggctaa aacatccatt gatccaaaac agctcagaaa cgcatttaaa
     5041 cgtatctgat accaatatta cagaaggagt gtatcgtatg attaacctat ttcttttagt
     5101 gacagctagt acattattct ttactggaat atcactgata atttcctatt tgatctataa
     5161 gaaagccgaa acttacacaa gcaaagcaac cggactagcc gtttcctcct ttttaacttt
     5221 gctgactttt tttgttactc tatcgttttc ttttgtttag cacagctgat ccatcagcaa
     5281 aaaacctctc tttacattat acacactagt gtgtataatg taattgtaaa caagctaatt
     5341 aacttttagg aaggagtgac aatatgctaa cagctacatt acagcaacct actcacgttg
     5401 ctattgcaga aagctataca attaatgcag gggtcgttaa acagctgatc aaggatcgag
     5461 aattcaaaaa gtttaacagt cgtcaagcgt ataacagaca gttaaagaaa gctattctaa
     5521 agcacaaaaa ttccttcata gggaaattgg aaagtgtgga tgttaaaact gtttcaacgg
     5581 cattttcagt tgttgaagtg actattgacg gtcgcttgat ttctctattt gcttttggaa
     5641 ttaattgata gggagtgtgg actttggaag gtaagacttt tgcagaaatt tggactgaat
     5701 ttattgaggt accaactcgc attgatccga taacttctga aaaggtattg gatgcagact
     5761 tctacaatct caaagcaggt actagccttg aatgcgttta gatgtattta gaagagcatt
     5821 tcaaaggcgt aacgctcggt cacgtcttat accacaatgg tgccgagtgg gatgcaattg
     5881 gaaatatcca tttttcttaa tacaagaaag gagaacacaa tatgaattat accgatgaag
     5941 aacaaatcac tttggaatac gttttcacaa atgaaaaagg ggaggaaatt gaaaaagtct
     6001 tgatcatttc atcaaaagac ttggagaacg ctttgactta tgaaccatac gaatcaaccg
     6061 taactgtcaa tgaattctta gatacctata cttctaatga tacttgggaa attatgaata
     6121 tttgcaacgc taataacatt caatttgctg aaaaaaccaa taaatcattt tattagtttt
     6181 gcaataaaac atcctctttt tctattgatt ccatacacaa caatgtgtat aatagatttt
     6241 gtaaagggag ggtagctatg cctaagacag caagagaaat tgtaaaaatt ctcaaatcta
     6301 acggcttcaa aaagactgga caaactggca gtcatgcaaa attttataat tccaagacta
     6361 aaagaacggt cgttgttccg atccacaatg gtgatgtacc actaggaaca gaagctaata
     6421 tctggaagca agcagggctt taacagcctt gccttcctat ttggtattag gcatactatc
     6481 ctctcttaca ctagaaaagg agcgaggaaa tgaagacaaa aaatattttt tatccggcaa
     6541 tcttcgagaa agaaggagaa gcttataaca tcgttttccc tgacttaccg gaaatatcta
     6601 cctttggaaa tagtcttgaa gaagcatatt taaacgctaa ggatgcttta ggattagcgc
     6661 tatatagtgt accagatgca gaatttccta aaccatcagc agtggaagaa ttaactttga
     6721 aagccaatca agtggtagta atcatccaac taaacattaa acttttccga agaagcttaa
     6781 atacaaaaac aattagaaaa aatgttagtg ttccggaatg gttagttctt ttagggaagg
     6841 agaaaaacat aaacttctct caactacttc aaaaagcact agaagaagag ttgtttgaaa
     6901 agtaaaaagg agaccgaacc tatgaaagct acacacttaa actatttctc tcgaacgtta
     6961 atcaataatc tattaaaaga tacaactggc attagcgtta gtaccaaaca ctttgccaat
     7021 gatctaatta tttttgagga taataacttg cgaattgaga tgtttattga acaggcaacc
     7081 cctaactcaa tacctgaatt ctgctatacg cacactgaaa aaatgaaaca tgcaaaatca
     7141 gttaaagatg ttgaagcatt aagtgaatca ctaacgaacg ctttaaacct cgttaagaag
     7201 gcaaaattgt tatttgaatt tcaaaccgac taccacaatg agttgcttgc tcaatctgaa
     7261 attttagatt aattgaaaca ggagaagaga aagatgaaac aaacacaaac agaacccatt
     7321 aacgatacgc ttgctgatca attagcagga tattataaat tatttgagat gagtaaagca
     7381 cagcgcttga tccactatct ttggaaatac aaaattttgt ttcttttttg ggaagcaaca
     7441 ctaacattct cagttctttt tttataagtc agattgttat aatcaacact tagttttagg
     7501 gagttttttg cttatctaca ccattttgat gcttaattta tatacctaca tcacacttga
     7561 agaacactta gtaattagcg atgcagggat cgagagaaac atcaaaaggg tatctttcaa
     7621 gatcacgtta aacaagccta ccgataaaaa gctctcagaa gcgttctgag agcttttgac
     7681 attttcttgt ataatttatc caaacacatt acaaagcacc taattcgagc ttatatcaag
     7741 caaatttgct atttattcct caatacatat ctaaaaatta cttgatggaa aactagatac
     7801 atggtaagca tgaaaagaaa atcaatttga gcagatgcag tataatacat agcaatttaa
     7861 aagctctcag aacgcttctg agagctttta atatattctt tgataatttg tctttcttaa
     7921 atccatacgt cctaaaacaa gctgtttttg cttaatatga tcatctgaga gacatcaaat
     7981 caacttttct ctcaacaaat cattcaaagc tttcgacaga ttaatattct cttcagccgc
     8041 taaaacattt aaatagtgtt ccacagtcac tgattttcga tcgtaatttt gcttgtttcg
     8101 agctttagca actttcgtat tagcttttac gacaaataaa ttaacattct cagatcgctt
     8161 gattcttgca tttaactctt gaacaggcgt aggcgatgga atttcttttt cttgcaactc
     8221 taaaagcacc aagtatcctt ccaatacttc ttgcgccatc tcaaaagcat cttctaaagt
     8281 ttcaccatgc gtatataagt ctggaaaatc tgggaattta actgcatatt catttccttt
     8341 ccattcgaat actgcacaaa aaaagtattt catcgcattg cacctctcta attaaaaaat
     8401 aatttttgct ttttagtttc aaaaaaagaa tcgcctacca aaaaaattga taggcgatca
     8461 caagctaatt aacctctaaa ggagattaat accttgctcc caaggcacta catatcttag
     8521 catacgtctg aagcatcttc aaacgagtaa gatatatttt gcattaacct aatttttttt
     8581 ctctataagg tttttcatca aactcactag aaatattaaa ctggacagca aaattgtaat
     8641 ttctgccttt tctaacttta gcaaaatcaa tcttcagcat tgatttttca ttaatttcct
     8701 caactgattc ccttaaaaca cgtctatcaa aattcgatat tgttttgtac tcattttctg
     8761 ttttcgtaat ctcacgaatt ttaactattt caatcagggg attcttatat atttcagctt
     8821 tctcaaaatc tcctttattt ttggcgataa gatatttttt ataatacatc gccaaatatt
     8881 tgtataacac aatgctatat tttttttgaa agcttgctaa atcttgaaaa acaaaatatg
     8941 gatatttctt tttgattgaa agaattaggg gaattacttc ttgcgagaaa ttgaaagtta
     9001 taatttgttc ttctaagtcc caatgaattt gatcaatagc agatactgta gcataagtct
     9061 tatattcttt atctaagttc gatatttcat aaactgcttg tctctgaagg gtgttaacta
     9121 tattttttag ttctgtttca agattttgat caacatccat gtatttgatt attgctttaa
     9181 caggtaaaac tatttcccct gttttcttta atttgttagg agagatataa ctgaacagca
     9241 attgataaag ttcatacgac tttaaatcca ttttcatgtc aattgttgct aagaactcct
     9301 cattgtactt tgtcagttca tcttttttca aaagtttttc cttccttcac gcattttttc
     9361 agttgttaat cataaaggtt cacataaggc aaataactcg taattgattt ttcgagatat
     9421 ttaacagtat tttttattgt gtaatcctcc ttatgagtag caacgtaaga taggtgattg
     9481 acaactgatt caaatcctct atctttggat aaacgatcat aaagcggata aactttattc
     9541 tgaagtcggc tcataagttt aagatcgcat aagtcggcag aactgatcaa aaagtttttg
     9601 attagtaatt tagtgtattt gctattcata gctttcaagt acgattgatt cattttctct
     9661 gttttatctt ctgcgccttt taaataagca gggtctcttt gttcttgctt ataaggtacc
     9721 ggaactgaca tggtacgtgt aagcaagaac tttatgtttg tataagtatg acccttcttg
     9781 ataagctcat agtcaatttt aaaatcagtg tatttattca gttgggcaat cggttcttta
     9841 attacacgtc tttcaaagtc tcgaaaagct ttatattttt tctcaatact aaatttcttt
     9901 cgtaaatctg caatggagaa aacaggattt tttaatctat caagtgattt tgtactccat
     9961 gttaagttat ctctatttgc ttcatacaga ttaaaatctt tagtgaataa ttgaaaaaaa
    10021 ctgatcgcat acttgcttgt caagttaact acttgatcaa atgtatattg cgtaaaatga
    10081 tcttctaagt tcgttaataa aggcataatt gaactactaa atttaataga tactgcatca
    10141 gaaaaatcat tgaattcagt aatttctagc ggtgagatga ctttgtattc agtcttaccg
    10201 ttaggtttag ttctttgaat tttaatgaca gattgctgat gcaatttttc aataacttgt
    10261 tttaatcttg aatgttttga agaagatgaa ctagaagaca tcaacgcaaa taaagccttt
    10321 ttagatactt tgacggtatt attaaaaggc ggatttttag ggtcaagatg tcctatagcg
    10381 taaagataaa ttttttgtgg catagcatcc atttcaacaa tgctatctac aagttgattc
    10441 gataaagtaa cagttaaatc aaactttttt agattgcttt cggtagtttc aaatttcggg
    10501 tttacattct tttctgttat tttcatacga ttgcctcttc tttctaagct tggtacgagc
    10561 ttaaaaactt ggaactggta cgattccatt gaataagtat agacctgttt tcgatacgtt
    10621 acaataagaa atgtatcgta aatttataac ataactcaat gtcagtaaaa cacattttat
    10681 tttttccaat caaatttcat tgaaaataca gcagaaatcg ctattttaga acattttcaa
    10741 aataaaaatc cttttcaata aaatacgaaa ttataaaaat taccaaaatt aaaaaacaaa
    10801 tatttttcta gtttttagaa ataaattcac atactttttc tttttttgga tatattcgga
    10861 ataccacatc gctttttgtc cacgtttgca tcgctttttg tccacattca catcgctttt
    10921 tgtccatgtt cgcatcgctt tatgtccacc ctaaaatcta tttaagattg atataaagcc
    10981 tttttggagg ttgctaaagt tttaaagatt aaaatatata ttataaatat aaataaaagg
    11041 ggaagctgaa gcttcctcct tttccttttt ttcaattatt caaagttaca caagcaaaat
    11101 aaactcctag atagtgttag ttttttttaa gattttttat ttttaagatt ttgaaataag
    11161 agaggagtaa tcctccttta ttaaatatct gttaatatat ctgttaaata tctgttaata
    11221 tatctgttag gggaggctta atctcttggg agagtaagta ttaaaggggt ataaaggtaa
    11281 cacttttgga cagtaaaggt aacaaaagtg gacagtaagg gtaacagttt tggacagcaa
    11341 aggtaacaaa agtggacatt tgaggtatca cactaggata agacgttacc ttcggttttc
    11401 ttgatttgga aggcgaaaaa atagaaaatt agattagtaa caaatctttg ttgactgtta
    11461 cctttagttg tgatttaatt taggtaacaa gcagaaaaaa ttgtttcctt taattagtac
    11521 cagattaaca tgattaaagg agcgcaaaaa gaagattgga gagtaatcaa tgtctcaaga
    11581 agttaccgaa aagattattg atagtgatgt agatttagta tcagaacaaa caaatgcaat
    11641 tgtgcgttat aacaatggtc tgaatacaac agctttgcga ggatttaagc ctgttgaaat
    11701 ggatgtgttt tgggctattt tatcaaaaat gaaacgtcaa ggaacagcag aagtaacttt
    11761 tgatttcaac ctctttcgtt atttggtcaa atacgacaga agagaaaagg acagttttta
    11821 ttctgtgtta aaagatttga gtgacaaact aggtaattta acctaccgtt ttgaagattc
    11881 agacgtctat gaacagctgt ggctttttca aaaattccgg atagataaac agaaggaaac
    11941 agttacaatt aaggcaaatg aggaatttga atttatctta aactcaattg gcaaaaactt
    12001 tactcgtttt gaacttgata attttacaaa actaagaagt agctacacaa aagaattgta
    12061 tcgtcatctc atgcaataca gaaataaaga gtggcgaaat ggttattggg tagtcaaagt
    12121 tgaagatttt agaagagcat taagtatacc ttcaagttat agaatgacaa atattgataa
    12181 aagaatacta gagcaggcga aagaagaatt tttagctcct gatgaaaacg gaatacaggt
    12241 atttgaaact tttgattatg aaaaaatcta tgcaaagaaa ggaagaagag ttgatcggct
    12301 cagattcact tttagtgagc cagaatcaaa cttgccaacc atttcaatgc ataattggtt
    12361 agaagaagat tgagaagaag caggttgtaa ttttaataca acctgcttct ttaaaaaagt
    12421 atgggtaaca cttttggaca agtcaagaaa aaaaacaatt tttcttaatt tgttgatcga
    12481 ctaaaggtaa cacttttgga caacacagaa taaaaagtcc ggtgatatca gttttggtag
    12541 gaaacatctt aatatctttg tgagattgaa ggtaacattt ttggacaagt caactatttg
    12601 ttaaaagtta atatagagta attagataag ttttttcttt tcgtctgata aatggataat
    12661 attagttatt ttattgctaa ttagtctttc atgatgactg aaatagctaa aataaatatc
    12721 tcgttggata attgctgata ctttaaggga tatctaaatt tgctgattga tttatttatg
    12781 ttattatgat attataataa cataaatgtg ttacgcccga acctcatttt aaaaaaatga
    12841 aggggtgaga caatgaatgc tattaattgg catgaatttg ttaactatat ggagagctta
    12901 ctagatgatc taaaaagttt tagtattgct gatgcagtaa agcatgggtt accagttagt
    12961 acaacgatga aattacgagg attgaaaatt ccagatgaaa ttattaagct gagaactaaa
    13021 acaattattg aaaccgcaaa agtcgttgac aatatgaacc atgaactaaa aggcaagaaa
    13081 acaacttacg agaaagattt tgaacgtttt ttaaaatctg ttattggaaa atatctaggt
    13141 aaaaaatctt tcagtgaatt gagagatgaa gagattgtac ctactccaat ggttagcaaa
    13201 atccgaaaaa acaaagccga ggggaattac tatcttggta caatagcaaa aatcattgat
    13261 tatttggaaa ataaagagca aaagaaagga acgaagaaag gaagataatt tttgtgttaa
    13321 atattaatgg ggaagaatat accgaaatga ttcaaaatcc cgaacctaga attaccgaaa
    13381 aaacgtctga ttgtttgctc ttttatgaaa gggggcagac aaactaatgg aaaaaaacgt
    13441 caattcaaca tctaccaaag atcaatacag tattattgga gggttgcttg gtgataagga
    13501 taagagaaca cgtcacggat tacgcattat gcttgcaact tttctaggag tgcttttttt
    13561 attaacaggc ttgggtagct acatttatca attaggaaac tataattatt atctcgtgaa
    13621 caatacaaca cctataggct cttcattgga gtttgctcga tcaggagcag agatcactat
    13681 ttcagatgtg tggacagatc aaaacagaga tgtaacagta gtaaaactcg gttactcgca
    13741 aaacgctaga aagttgttat ctacgcaagg aaaaaactac aacttatata tggcaacgaa
    13801 agaaaaagat cgtcctaaaa agcttgaaat gagctatggg atgttaagta ctgatgggga
    13861 tgctttctta ttcctaaaag gtaaattaga tgaaagagct tatcaagttt ttattgcaaa
    13921 ccaagtatca ctaatttctg gtgatgatac agctgacgat gataccaaag tcaatgtgaa
    13981 tgaagcagat tcagttacaa aagcactttc tcaatattct atgaatgatg ttaataatga
    14041 aggagtaatt tcttttggga aaaaagcaaa acctgttaat actcctgata atatcaattt
    14101 cagaataaat ccatattcag attcaactaa tgtctatcaa ggaagctttc ttaccaaaga
    14161 tggcgatatt gattattcaa aagttgtagc agttacaagt aagaataatg ttgtaagtaa
    14221 gttagaagat cagttaaatg cttcaaatac taacatcaag aaaattgaag ctacaaaagc
    14281 tgagtatctg gaacgtttac aacaaaatcc tgacgataaa gattcgcaag aaagtattcg
    14341 tcaactagac agtcgaatgg agaccgaaat taaagcaatc aatgatacac aaaaattgat
    14401 tgatcaatac aaaaatgctg actttaacaa agattctttt ggagacatga agacgacatt
    14461 taagtaccta actctgactg actagaagta aattattctt attttagagg aagtgaacac
    14521 aaacagatgg aaaatagtac cgagtttttt aagaatattc aaactgaatt taatcatgct
    14581 ctcacaccaa tgaaaccacc gaaacataag ttagtgggac gtgatgacga actccatgag
    14641 ttgtcactga tcttgaatag accggaaaca ccgcaagcta tcttgttagg tgaagccgga
    14701 acaggtaaaa cagcacttgc aagtgaatgg gctgaaagac agcgacaaga tggaaagaaa
    14761 gtacaagtat acaaattatc aatcggacaa ttgcgtgttg ggggagatga tgtcctccaa
    14821 acacgtttag aaacattaat tccaagattg aaagaatatg agcgaatttt aaaatctgag
    14881 gacaaagaaa atgaatttgt cctcttcatt gacgagtttc ataaagttgt tagctctttt
    14941 ggtagtggca ataaaattgg gggtgattcg ctaaaagaac cgttagcaaa tgaatcactt
    15001 caattgatta ccgctactac aagaaaagaa tatgaaaact atatcaaaag agatgatgcc
    15061 ttagggagac ggttacaacc gttagaattg aatgaactaa aaagcgatgt tgtcgtcgat
    15121 attttgagac aatggctaaa tacatcctct gttaaagatg atcctcaggc ttcttttaaa
    15181 ttatcgcaaa ggattcctga tgaattactt caagaaatcg tacaagcaaa caagctttat
    15241 cgtgaggatt tgtccgaacc ggctaaatca attgccgtaa tggaggattt gattgcaaaa
    15301 gatcgagtag acggaaatgc ttataatcga gaaagtctat tgcaaatatt tggtaagtat
    15361 aatattcagc tagattctga ttttgatatg gaaaaacttc aagaggaatt aaagtccata
    15421 aaagggcaac cgttagcagt ttatactata acaatgttgt ttaaacgtat gaagcttgga
    15481 tttgatcgtt ttaaaggtcg aaagcaacca aaagcaactg ctctgtttac aggatcaaca
    15541 ggggtcggta aaactgcaat ggctaaagcc gtggcaagag gactttatgg cgatgaaaat
    15601 aaagttctct tagttaatat gccggactac aaaacaaaag atgatgcaat gcgttttaga
    15661 cgactcatag gtactcatgt aaggcatgat ccttctgcga ttattgtgtg tgatgaatat
    15721 gagaaagctg gtgtagaaac actcgatgcc tttttatcaa ttttagatga aggtaggata
    15781 agttttactg aaagaggatg ggatgatcga gaagttcacg acactgtttc attgtcaaat
    15841 acaattttat ttgcgacaac aaatgcaggt gcggagatgt tcgattcgtt aaacagatat
    15901 agctcactat ctcgtgttac aaacaaggaa aattctaatg aatatagcac tgaactaaaa
    15961 agagaatacc gaaaagtcgc atcagggatc aaacaagcct tgattggtga aggtctaaag
    16021 cctgaagtta ttaaccgatt tggacgaata gttccatttt gggctttatc cgaacaaact
    16081 cttctggaaa ttgcttataa tgaaatgttg aagagtgctg aattagtacg aaaacattta
    16141 aatatagatt tacgtcttcc acctaggaaa gaaagagtat atggtcaaaa tttttatgca
    16201 gacgatctct ccatgcactt agtttttgac atggcttcac aagaggattc taaatcaggg
    16261 ggtgcaagga atatcttgca tttagttgaa tctgagtttg aagaagcgat tactgaagca
    16321 ataattactt atccaaacgc aaaaatattc gatgttaagt tgagtgaagc cggagcgttt
    16381 gaaggaaaag gtgattctaa tgctgaaatt attgcaaagc cttccgttgc tgaaagtaaa
    16441 gaaaacaaga ttgtattttc gattggaggg gcgtagctat gaaattcaag aaatatttac
    16501 tacaaaacct tagaataaaa cgatgggtcg taagaggtct agttattgga ggagtattag
    16561 cagttacttt aggcgctcct tttggaatga atgatccaac tcctgacaat caagatagca
    16621 aacaagaaac agttataaat aagaacgttg catatgcgga aagtaaaccg gcagaacaag
    16681 gaatgtggac actatatgct cgtatcctaa tgggagataa agaagttaat gatagtgccg
    16741 ctaaaactgg acaaatgaat ttgttaggac aaggtggtat cagtgccgat ttcccttatt
    16801 ctgacatttc taaacaaggt aattcgattg cagggggtaa tgcaggtact caactagcat
    16861 ctactttagc aacttatagt tactacggat atattgaaac agtctcagga aatgctattg
    16921 ccagtcaagc atcatccatt ctaggaagta ttggtaggac gattggtagt tttatcggat
    16981 caattagttt aatcctttct aatattgctg aatccataaa tgaactcctt ggaaaatttt
    17041 tcatgagtat gaatatattt tctcttatgg ggtggggaga aggtggatcg gatgtatcta
    17101 atccggtatc taaagcatta actagcttgc ttaacaattt gggattaaat aatgaattat
    17161 ttaaagcgat agcaagctta tctatgctct ttttcacagc aatactaggt tatcaaggaa
    17221 tgaaattatt gtcgaaagat ggtgttaact cgaagggatt atcaccagta cttgaaaaat
    17281 ggattgttcg tatttttgta ttgtttatat tcttaccagt ggcaggatcg ctattgggag
    17341 tttatatcaa cggcatccag tcagcaaaaa aagatggaga tataccaaca aatgttgttt
    17401 ctgactacat cattaacaca agactttggg cttctactca aaatcttgct ccatcatctg
    17461 atactggtgg tgatgtacct gacggaaagc ctacatccga aatcaagttc gttgatacag
    17521 attatgaacc atctaaaaaa tctgcaatga taagagcgat caatactagc agttatgcca
    17581 gacaaaataa tgatctagat tctaattcag tcggtatggc tctattaaaa gaatggtcat
    17641 cgaatgatac tttcaatatt aatacttatg ccggtgatgt taatgtcgcc gaaaatgatg
    17701 cttatatggg aagagcgcct gctgtttgga caggctataa tttaggggat ggaaaagaag
    17761 gaagtgcaat taagaaacca accaatttaa atggatatat ttggtcagca aatcaaaatg
    17821 tgacagacga caatcgcaag cctgatggaa aaggaacagc gcataacttt gatgctactc
    17881 aaccttgggg cgttgctaac aatgcaactt ttagcacaca gtcggtagct ttattgctcc
    17941 aatcggcact tgatacatcg ggagctaagt tttatgcgta taatatcgct ccttcaggaa
    18001 ttcaaggcag tgctaagaat atgtcaacag taaaaacaga atggcgtaca gcatccttag
    18061 ttggtcatgg agctatgggt aagtttggct cgtggctagc gatgaacgct gaatcaatcg
    18121 ttttagcagt tctttactta gcatgtgctt gggcgatgtt caatgtgaat atcgtgaaag
    18181 catttaaacg tgaatttatt cggatttggg aagtagttct aacaggatca cttgcatcag
    18241 gagcaggtgt gatggcaatt tcattaggtg ttgttacaac atctctgatc ggactacaat
    18301 tacctatctt tctgattact tttattcgtg catttggtga tactgctgtg ggattcattc
    18361 ctaatacatt agttggcgat ggcgtagcaa atattgttcg atctgctatg attttaggtg
    18421 taggatatct tttagtcttt aaaaaaggcg aaaaagtaaa taatttgatt aatatgctta
    18481 ttggcttacc acttgaagta gcgtttgggt ttgatgaacg tgctaagtta ttatttggta
    18541 atagaaatga tgctgtgcga gttgtggacg gcgtttctaa agcaatcaag aaaagccaag
    18601 gagctaaaaa tccctattat gatggtggca acagctcagt aaaagatatg gttgcaggag
    18661 gtcttggagc agttggagcg caaaaaatgg ctgaacgttt gaacaatatg ggagatagca
    18721 atgctgatgc taagtccatt gctgataaag taattcctga aacaccagaa ggaatgtcat
    18781 tcaacggtgg cggagttggt tccacaatca ataatacctc tttcaaagat ttagttcgtc
    18841 aagatcaact acgtaataac tcccaacaat atattgatca tttaggacgt atggttgatg
    18901 aaaacggaaa ctttgtcgat gaaaatggtg aaatattaaa agatagtaaa tacggtaaag
    18961 atgctaaagg caactttatt gacgaaaaag ggcgtttggt cgatgaagat ggaaacctta
    19021 tagatactaa tggtaaacca ttggaagata gtgaagatgg taatattggt atggatagcg
    19081 aaggacgttt aattgatgaa aaaggtcgct tagtcgatga agatggaaat cttgttgacg
    19141 aaaatggacg caggttgcta aatggtgatt ttggtcatga tagtaacgga cgtttagttg
    19201 acgaaaaagg tcgtttagtt gatgaagatg gaaatcttgt tgatgagttt ggtaataaac
    19261 ttggtaaaca cagcgatgga tataatgtga atggagattt agtcgatgag gatgggaata
    19321 tcattgagac gagagatgcg cttgtggcag atcgaaatag agaactcgaa caacaacggc
    19381 atacgccaat tgctgttgca attcctgaga atgaagaaga cgagattact ttagatcaaa
    19441 ctcatcaaga actgaggaat gaaatgagtg gtgaggacgt gtcagacaga gtgcatcggg
    19501 aattaggaga tggagcatct ggtgaaagtc agtcaagaga gcttaacagc aactttgata
    19561 atagtgctat tcggaatgac acagtactta aagacagaga atcgctaaca aaagaaacgg
    19621 ataaagaaca atcagatgtg aagaaaaagt tcattcctac tcctaatgtt aatcaaacag
    19681 ataaatatga aacaaatatt ggatcaggaa tgtcgaaaga caatcaatca attagagaac
    19741 ctgaactgac aaataaaaat aatagaacaa ataattctaa atctacttca gataattcag
    19801 ggaaatctaa tcaatcaacc aagaaaacat caacagctaa atcagccgtt gaatatctat
    19861 caaaaactgg taaaaatgtt tacacctcag cagataaagc tttagcaaaa gctccaaaag
    19921 gtagtctagc agggataacc tatgaagcta aaaacgaagc tattaaaata ggtaaaggaa
    19981 cggctaaggt tgccaaggct actgctacag gagcatccaa aactggtaaa gtagtgaaga
    20041 aatctaccat gtcagtcaag gactatattg taaaatacgg taatcaaaaa tcaactgaaa
    20101 ataagtctga agacgagtaa aaagaaggag cattgatttg ctccttcttt ttactttgat
    20161 ttctaactga agatgtcaag gaaaatttct atttatgcaa aaaaattatt ttaaattgtc
    20221 tataaatgaa ttaaatattt actgttcaaa gttgtataat taaaaagggc ttatgctggt
    20281 aaagaacaga attattatct aaacaaaaac ttgataaacc agcattttta gccaataaaa
    20341 aaagtgaaat aatttaatgg aaaatctctt ttaatcatcg aaaatttatg ttattatgat
    20401 aacataaaga tgtaaatatc gagacgtctt tttcttagtt agatcattga caagtgaaca
    20461 aataagattt gtcgatgata taattaactt attaaaaata ttaaaaagga ggtttttata
    20521 tgttcgattc tattaaaaat ttggttagcg gaatgacgcc tgatatgaaa actttagtac
    20581 aagttgtttt gtatttcttg gcattcgttt tttcgttgaa accaggattc actgcaatga
    20641 aattttttgg tgatgcaaaa tggggagatg gattaaaata tgttggggcg attattgcaa
    20701 tctttattat tcctgtaatt acaaccgtta tgctttacac aatgggtact aaaacaggta
    20761 atgatttgaa ccaaaaagct aatatcatat cagcacttat tccagttgtt ggtatctatc
    20821 tagtttcaaa atttccaaaa aatgaactgt taggttaaaa gtgaattgaa caaatttgga
    20881 taatatgccg atttgttcaa tttacttttt accaaaaaac tacgctccaa gcgtagttta
    20941 gaagtgaggg aagaaagtaa atgtaagatg gtttttgata gagaaaaaat gttagctcac
    21001 gccaacgaag tattaatgag cagtctgaaa ggtaccgaac tggcaaagat aatgaatatg
    21061 aatgttaacc agttctatga ttatcgaaat gggagtaaaa agattgaaaa agccagactt
    21121 gaaacgctta ttaaattcga aaaagcatat gtatatatgc tagataaaca gaaaagaact
    21181 attgactgaa aaaaggagtt ttacaataat ggcaaaattc aaagatatta ccgaatctat
    21241 taattatgtg gcagaaacag ttcctgtagt tgcactctct ttgaagccac aattactcga
    21301 agtgtacaaa aaagaagaaa aagaagcgaa ggcaaaaaaa gctaaagcaa aggtatggaa
    21361 agaattcgat gcgttgctga ccaaagattt accaaagaaa aattatatgg taattgaaga
    21421 tgtacaaaag ctcaacagtg agggagttat agatgaggat gatgctattc tcgattctga
    21481 gaagtatgtc gttctattga agtcaagttt gaattatgta gctgatctac cagaaaaatt
    21541 taaagagaag ctattagcag gtgtccgttt tatggatagt agtggtcaat tattagttat
    21601 tccggatgat cctaatgtgt ttgaatcaga agttgaagaa gctacagact tctatagcgc
    21661 agtagagcaa ttagaagtag ctactaatgg attagaacga attgctgaga ccttcaactc
    21721 ttctgaagaa gttgaatttg aagccgtagc agaaaatgta ccagatgaaa aagaagctct
    21781 ttttgtggat gataacaaac caaccgaagc agaaaaccaa gaagtaagca tagaagaaac
    21841 acaaatacaa acagaaacct accaacaacc aaaaattgat gaaaaacccg aatcccaaag
    21901 tgacaaagaa aattatgttt atgaagctga aaaaatcaca tctgaaagtg atgtaccgaa
    21961 aaacttagat gctgattttt tagaaaaaaa ttttgaacag atcattgctg acaaatttca
    22021 acctgtagtt ttagatgaga ttaatgaccc tgtcgattta aacgaggaga tgctatccat
    22081 acctgagttg aaaccattgc tgtttttagc taaagagagt atgaacgctc gcatctctat
    22141 tgctaataat aaaatcaaca gtgaattaaa taagacgaag gagcaattga tcctcaaagg
    22201 aaagcaaatg ttagctgatg agcttttgcg gatagaggaa gcaacctcat taaatggtca
    22261 aaatcagttt acaaatacac tatcggttgc taaagagagc tacgaagcag aagcatctaa
    22321 aattgatgat ttagtgatgg aaaaatccaa tatcattcgt aaagattatg atatggcgaa
    22381 acaggcttat ttggatgctg aactaaagaa actagagcaa cggtatgatg ctgagcattt
    22441 accagaagtt aaacaacaag aagatgaata tagaaaagca actgaagaaa gtcttgatca
    22501 acagtaccaa gaagcaattg aatctattaa aaacgatgca ttgaaacaaa gccaagaagc
    22561 aaaagaagtt gtggtggaac gaattatcaa ttcattgaaa ccagaaattg aaaaatcatt
    22621 aacagacgtg caagaaaaaa ctttagagat tgtcgaaaaa acaaagtcag aaaatgaagc
    22681 tgagttccat gatacaaaag agaaaacaac agatgtggtt gtcaaatatt ttgcgaacaa
    22741 agaagaagtt gaaaagcagg tagctaacaa cacgaaagaa ttagaaacaa aacttgtaga
    22801 gcaacaaaaa actatcgaag aatataaaga tcgaatgaaa gaggtcaaag atttagcaga
    22861 gggttataaa ttaaatagcg aaaagttggc agaacaaagt gaagctttaa gagaagactt
    22921 atctcgttcc cgaagtgatc tagctcaaat ggcagaaaaa acgaaatcat tagcagttgc
    22981 tacagaaaat caatcaagtt caaaacgtga ttttacaagt aaaaagatgg ctactatcat
    23041 ttctgtcgct gcaatggctt gcggaacgat cttgggatcg tcctacttta ttagtcataa
    23101 taatcaacaa gcacaggctc aaactcaaac atctatttcg agagtggaaa attcgattgg
    23161 gacaaatacg aactctgctt caacaaatag tagctatgaa gcaacaccaa ctacagagtt
    23221 caaagttggg aaagatgttg tcatcagtat tgatgggaaa acaactaagg ctgaagtgat
    23281 ttctattgat ggagaaaaag cagttgtgaa aactccagac ggaaatcagt acaaaattca
    23341 agctcactta tctgataata actaaggagg gctattatgc gagaagaaaa aatagtttat
    23401 ccagtaattc aaaacaatca agaaaacatg acctcttctc agcacttaca ggaagcacag
    23461 ctcgtgtatc aggtaaatga actaccacaa acgaacgaga caatgagcaa gtcaatatct
    23521 ttaattggct tgcttattgt tgcaatagtg ataactgtag gtgtttaccg taagttgaac
    23581 gtgaaaggaa gtatttctga tggcagataa ctcaaactat ggatttaaac ataacttaca
    23641 tcaaaaacct tctgaaacaa ttgtcagagt tgaatggctt gaaaagcatg tcaatagcac
    23701 ctattctgtt acagcaaaaa gattaattgt atcagctata gttgttgcag tcgttgttct
    23761 tggatcaatt gtttacttag tttctgatac gattaaactg aaatttattt ttgttccaac
    23821 gttattaatg ataattggat ttcttattct aattagccag tatatgttta ttgatccatc
    23881 tagtggttca ttgatttctg gttctattag actgttcaaa tcagtagtta attcagcaag
    23941 agtatctatg gggaaaaaga aaccatttcg aggcacagag attttaaaag tgaaacaaga
    24001 tggaacaatc gtatatcgag atgggagtgt agcattaatg ttcaatctag atggggctac
    24061 atcactaacc gcttttccag aagaagttat ggctcaggaa tcgaaatcaa tacaatatca
    24121 aaataccaga gatcgttcct tagctgaaat caaaatcact tcatctcaac ctcagaatac
    24181 ggaaagacaa actcgaaata tgaaaataca aaaacgaaat gcaaggagta acaaagcagt
    24241 ctttgacata atagatcagc aagaacgatt tgtatcagaa tatattgatg gtgtgaaaac
    24301 aacagtaatc caatatctta tcttgaaatc tcaatctaag atggtattgg atgaaggtgt
    24361 ggaacgttta gaaaactata cgagtagagg actttattat agtgtcactc agttgtcagc
    24421 taaacaaaca aaagaaatat tatctgatat ttatgcactg aaatagaggt gagagattaa
    24481 atggaaatta tgggtagatc acaactatta aatttcaaaa aaaagagaaa aactaaaaaa
    24541 gaacaaaaaa ctagaaattc aaaggtagtg aataatacct ttgttagtga tttaacatct
    24601 ttagttggtt atcaatttac accatcttac attaggacag gtaagcggtg gggaacaatt
    24661 gtgaaattag tgaatactta tggaatgaat agaaattatg gatttggttg gatggttaaa
    24721 gctattcctg aagtgagagt agaaggggta aaagcctatc taattgaagc agataagcca
    24781 gtgtcacaaa aagatcaaca agaaataatg cacgagaatg ttgggggatc aacgaatagt
    24841 tatgaaaata atttagaagg aaaaaattca gatgctgaaa ttagcaaaac tgttatgcgg
    24901 attatggatt tgaatacagc atctttacta gacagcatgg caaataaaat tattgattgg
    24961 aagctagaaa tcctattagt atccgattct cctaataaaa tcagcgaaca gctaattaaa
    25021 ctccaacggt tgtatgatga aaaaatgtat ggattaaagc ttatgtcata tggaggagat
    25081 caacgtgatc tgtttgatag acttttaagt gaaccaatcg ggaatgtcta tgagtatacg
    25141 tctatgagtt cggattttgc aggaaatgat catgcaattc gcaaaggttt agatgatgcg
    25201 aatggatggg ctatcggcac tatgtctact agctacgcaa gtggtgtagc ttttatggca
    25261 ctggatgatt cttttacaag taaagtttta gtggcatcac acaaaaacgc tacaattaaa
    25321 ggctatgatg aaaaaatctc agcatcatca ctttgggggc aattgattgc taataatgca
    25381 atggcaaatc ataaacgaac ttttcatatt gtcttaaata aatttgatta ctttggtgat
    25441 ccaactggta gaacatttgt tgccaaacct tcgttaaaca gtgtaataga aaaggttaat
    25501 ttggctgaag aaggtcttaa ctctttggag ctttttggta atcaaaagga cattggtgat
    25561 ttatatagac ggaatctcga taaactaact cagatttttt atttgttagc aggcagaaca
    25621 ttagattctg caactagagt tttattgaaa aaagaattag gtaactttta tataagtcag
    25681 aaactttggg atattaatta tgaagaacgt tcaaaacttt tggtgaacag tagtgataca
    25741 atgccagttc ttggggattt tattagctat ttgactaatg ctgaaacaaa agccaaaaaa
    25801 tctggtactg aaaaagaagt cgatcgtatt attgtattaa aagacacatt gagaacagca
    25861 ttagacagct tctctaggat gtttaatcaa agaacgacac tgaaagaaat cggtaaaagt
    25921 gacaaattac agttttacta taacttaggt gatctaagag cagaaaccaa tgttatggaa
    25981 gctcaatttt taaatacctt tgatttcatc acatcacaag caaaaaaaga tgatattgtt
    26041 atgattcacg gcttcgataa gttatctatt gaaaccgttg ggctaattaa aaaaagatta
    26101 acaatgcttg aagaagatgg aattcgtctt gcctttctat ttgatacaat cggttcagga
    26161 gaagacaatg aatacaaaga agatattgaa acagctgata tattcaatac agatggaaaa
    26221 atttaccaaa gttttgaaag tgattttaat tacactatca ttggaaagat gaccagtgga
    26281 gagttaatga agtatgaaaa aaaagttgct cctgctagaa aaggagaaga tgatcaaaaa
    26341 ctttctgttg atttgagaca gtatttgaca acaggaagaa gtaataaatt tcagatcagg
    26401 agaccagtgg atctgactaa taattttgtt attggagagt ttattcttta agttttatct
    26461 ttgattttga gtgtataagg aaaggataag aaaaaacatg tatatttgag actaaaaaca
    26521 gtcttagaga acatggtacg ttttgtgaaa agagaactat agaaatttca taaagtgaaa
    26581 ccttattgag tgaaataatt atagtggagg ttaaataatg aaaccaaaca taaaaatttt
    26641 agatcgaata tttctaggta gagacacaga agtaatccta attcaacatg aagaaggatt
    26701 tgaagttagt ataggtattc agaaacttca aaaacctcat tattgtaatc agttgtataa
    26761 aaactttact gatgaagaaa aagcgagagt tttttttaat gaagtcaaag gtatgagaga
    26821 acagtatgag gtggttgaag catgaataag ttaccagaaa agaaccgtgt atttagattg
    26881 aaaggtgatc tcag
//
LOCUS       DACDHL010000032        26268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDHL010000032 DACDHL010000000
VERSION     DACDHL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.032"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(81..2252)
                     /gene="copB"
                     /locus_tag="IV228_001374"
     CDS             complement(81..2252)
                     /gene="copB"
                     /locus_tag="IV228_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8717300.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2272..4458)
                     /locus_tag="IV228_001375"
     CDS             complement(2272..4458)
                     /locus_tag="IV228_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8717301.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4458..4667)
                     /gene="copZ"
                     /locus_tag="IV228_001376"
     CDS             complement(4458..4667)
                     /gene="copZ"
                     /locus_tag="IV228_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8717302.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4680..5120)
                     /locus_tag="IV228_001377"
     CDS             complement(4680..5120)
                     /locus_tag="IV228_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8717303.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5194..5733)
                     /locus_tag="IV228_001378"
     CDS             complement(5194..5733)
                     /locus_tag="IV228_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP8717304.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6348..7814)
                     /locus_tag="IV228_001379"
     CDS             complement(6348..7814)
                     /locus_tag="IV228_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8717305.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8125..10206)
                     /gene="cadA"
                     /locus_tag="IV228_001380"
     CDS             complement(8125..10206)
                     /gene="cadA"
                     /locus_tag="IV228_001380"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8717306.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10348..10527)
                     /locus_tag="IV228_001381"
                     /pseudo
     CDS             complement(10348..10527)
                     /locus_tag="IV228_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(10730..11089)
                     /locus_tag="IV228_001382"
     CDS             complement(10730..11089)
                     /locus_tag="IV228_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8717307.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11119..11460)
                     /locus_tag="IV228_001383"
     CDS             complement(11119..11460)
                     /locus_tag="IV228_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717308.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11457..12131)
                     /locus_tag="IV228_001384"
     CDS             complement(11457..12131)
                     /locus_tag="IV228_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8717309.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13308..14606)
                     /gene="asnS"
                     /locus_tag="IV228_001385"
     CDS             complement(13308..14606)
                     /gene="asnS"
                     /locus_tag="IV228_001385"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8717310.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14646..15836)
                     /locus_tag="IV228_001386"
     CDS             complement(14646..15836)
                     /locus_tag="IV228_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8717311.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15857..16384)
                     /locus_tag="IV228_001387"
     CDS             complement(15857..16384)
                     /locus_tag="IV228_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8717312.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16431..19220)
                     /locus_tag="IV228_001388"
     CDS             complement(16431..19220)
                     /locus_tag="IV228_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8717313.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19367..19567)
                     /locus_tag="IV228_001389"
     CDS             complement(19367..19567)
                     /locus_tag="IV228_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8717314.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(20019..20339)
                     /locus_tag="IV228_001390"
     CDS             complement(20019..20339)
                     /locus_tag="IV228_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717315.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(20723..21841)
                     /locus_tag="IV228_001391"
     CDS             complement(20723..21841)
                     /locus_tag="IV228_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717316.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            22312..23619
                     /locus_tag="IV228_001392"
     CDS             22312..23619
                     /locus_tag="IV228_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8717317.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            23738..24937
                     /locus_tag="IV228_001393"
     CDS             23738..24937
                     /locus_tag="IV228_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8717318.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(24964..26232)
                     /locus_tag="IV228_001394"
     CDS             complement(24964..26232)
                     /locus_tag="IV228_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8717319.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         7407 a         5669 c         4606 g         8586 t
ORIGIN      
        1 agaatgaggt tcattctgac ccagatttga ttttgtacaa cccatcagtc cttattgaca
       61 aagagcccct tttttcatta ttatttgatt ttcaatgtca aagcatttag agctacaact
      121 accgtactta acgacatcaa gacagcacca actgctggac ttaatatgat accaattggc
      181 gcaagaattc cggctgctag cggaattgca agaatattgt aacctgcccc ccaccagagg
      241 ttctggatca ttttctttcg cgtttgcttg gctagatcaa gaaaatgcaa aatatctttt
      301 ggatcgctgt ttgtcaatac gacatccgca gaatcgattg cgatatctgt ccctgcaccg
      361 attgccatcc cgattgaagc acgggctaaa cttggcgcat cattgatccc atcacccacc
      421 atgattactt ttttcccttg gtcaaggtat ttctggacaa cagcttcttt atcatctggt
      481 aataaaccgc cataatattc ttcgatccct aaataatctg ctactgcttg ggcagcgttt
      541 ttattgtcac cagtaagcat gacaggagtg atttttcttt ctttcaatgt tcggataaat
      601 tcttttgctt ctgtttttac tacgtcacct aaagcaacga ttgcagctag atgattttct
      661 aaaatcagga agctgatggt atttccttgt tcttggtaag gtttcaataa tgcttgttct
      721 acgtttaatc cgagacgctc aacttctttt tcattaacta ttttcacttg ttgttttttg
      781 actgttgcct ctagcccgac tcctgaaaga ttttttagat tttcagcttg ataaggcttg
      841 atttcatgtt ctttcaaata attcataatg ccaacagcca atggatgatt ggcattcgct
      901 tccaaggctc ctagatattg aaggatttca tttttattga acgcatcatc aagtacttcg
      961 acacccgtta cagtaaattt tccttctgtt agtgttcctg ttttgtccag catgatcaca
     1021 tctagatcat tggcttgctc cagtgcattg cggtttttca aaagtaatcc gtttttagct
     1081 gctattgatg tggagcgagc aacaactaat gggatagcta aacctagagc atgaggacag
     1141 gcgatgataa agactgttac catacgttct aatgcattag gaagatctgt caagaacaac
     1201 caggcgatga atgccaggat acccacaatt aatgcgacat aaaacagcca ttttgccact
     1261 ttgtcagata atgactctag cttggatttt tctccttgtg cttttcgcac catttccata
     1321 accttcgcta gataaccatt ttcaccagtt ccagttactt cgatttcgat cgttccatcc
     1381 ccattgatag aaccgccaat cactttgtca ttggcttgtt ttttgattcc tcttgattcg
     1441 ccagtcacag ctgattcgtc aacaattgtt tcacctttca agattacacc atctgttggc
     1501 attttatcac cagaccggac gatcaaacga tcaccttcgt ttacttcttt caatgaaact
     1561 gtctcttctt ttccatcttt cgtcaagcgt ttcactgatt ctggcagtaa ttctgctagt
     1621 ttttgtaaag cattgctggc attcgacaca gcattcattt caatccagtg acctagtagc
     1681 ataataacga tcaaagtagc caattcccag aagaaatcca tgacgtgtgt atgtggattg
     1741 agcagatttg cgataaacga atagacacta taaatatatg caactgtaat ccccatcgcg
     1801 atcagtgtca tcatagcagg acttttttgt ttcaattcca tcttagctcc acttaaaaat
     1861 ggctgaccgc catagataaa tagtatagtt gctaagatca atacgagcca atcagaacca
     1921 ggaaacgtta cttgaaatgg aaattccatt cccatcattg gtgaaaaaag aatgattggg
     1981 atagctagga taagcgatag ccaaaatttt tgtttgaaat tccccatgtg catggaatga
     2041 tccatgccgc cgtgttcgtg atccatgtgc atcgaatggt ccatttgttt gctgtttgct
     2101 atatctccat ggttatgtcc catgtgcatg gaatgatcca tctgactatg gtcatgtttc
     2161 atctccatct gtttttcatg agattctgta ctttctttgt ttactacttc ttttttatgc
     2221 tcatgttttt tctccatatc gttcccattc atttttactc catcccttct cttacttttt
     2281 catgtgtcga tttaagctca gggagttcaa caatacactg acggaactaa acgccatcgc
     2341 tcctcctgca atgattggat tcaaaaagcc tagtgcggca aaaggaatac caatcgtatt
     2401 atagacaaaa gcccagaaaa gattttgttt gatttttctc aatgtcaacc tagacaactc
     2461 gattgtttgt gcaatggatg ccaaactgct attcatcagc gtcacatcgg ctgtttccat
     2521 cgcaatgtcc gttccacttc ccattgccat accgacatca gctaatgcta aggccggtgc
     2581 atcattgatt ccatcgcctg ccataccgac ttttttgcca tctttctgaa gtttttctac
     2641 gtaggctgct ttatcctctg gaagtacttc agcaaagata tgctttggat caataccaac
     2701 ttgtttacca atcgtttcgg ctgccagttt gttgtctcct gtcagcataa aaacatctaa
     2761 tcctttattc tggagctgct tgattgcttg tttggcttct agtttgattt gatcagccac
     2821 ggcaatccac ccgatcactt gcttttcatc agctaaaaac atgactgttt ttccctgctg
     2881 ttctaattct aacgcgtgtt cttggtattc atcaaatgat aggttcaatt caattaatcg
     2941 tttacgtgta cccgcaaaat agcgtactcc attaatcgta ccgctgatcc cagcacctgg
     3001 gtgggctgta aaatcaatca tatcgtatgt tgctgcttct tgcagttttc cgtattccac
     3061 gattgcttta cctagtggat gttcagaagc ttgttctaaa gtataaaaaa tagataggac
     3121 ttcttttgaa ccgactagat ctgtaacttt gggctttcct tctgtaatcg ttccagtttt
     3181 atccaaaaca atgctgtcta agtgagcagc tgcttctaat gcttctccgc ctttaattaa
     3241 aattccattt cgtgcaccaa cacccgttcc taccataata gctgttgggg tcgctaaacc
     3301 taatgcacat ggacacgcaa tgactaatac agagacacta tggatcaatg cgagttgcca
     3361 atcaccagtg atcaaacctg tagctatgag cgtgataaac gcgatgacta atacgatcgg
     3421 aacaaaaatc cctgagatcc gatctgcgat tttttggatt ggggctttgc tcccttgagc
     3481 atcttccacc atttgaatga tttgcgctaa tactgtctgc ttgccgactt gtgtgacttc
     3541 catgtgtagc agaccattcg tattgattgt cccgccaaaa agcgtgtcat ccggattctt
     3601 ttctacagga agattttcac cagtaagcat actttcatct atcgcactgc ttcctgaaat
     3661 aattcgacca tctgctggaa tctgctcacc aggacggatg actagttgat caccaacgac
     3721 tacttcttca atcgctaacg tttcttcttt cccgtttctt atcacttggg cggtttttgt
     3781 ttgaagagac atcaattgtt tgattgcatt acctgttttc gtttttgctg tatgttccaa
     3841 atatttgccg agcaaaatca gcgtgatgat catactgctg ctttcaaaat aaagatcttg
     3901 ggggtgtcca cggaaaaatc cgttgtaaat gctcaatgca aaggcagccg atgttccaat
     3961 tgctaccaat acatccatat ttggtgcttt cgtcttgatt gcatggtacg cacctttata
     4021 aaaacgagca ccgacataaa attggacagg agcagacagc actaactgga cgattggaaa
     4081 atgaaaaaag tgaacaatcg ccgcatggct tcctaacatc atagcaatca ttgacaacat
     4141 caacggcaaa gtcaaaaccg tactcaatat aagatcgaat agcatttttt tcaaatacgc
     4201 ttgcttttct tctgcaattt tttgtttgtg ggcttcatcg tacaaaattg caccataacc
     4261 gatgttttct acgctttgga ttaatttttc agtggtcgtc ccttcatatt tgacacttgc
     4321 tttttcagtg gccaagttga ctgtggcatt gaccacacca ggttgtccgt tcagctcttt
     4381 ttcgatccta gcagaacaat ttgcacaagt catacctgta atgacgaacg tatccatttt
     4441 ggtttctgct gttgccatca aatcacctct gctttataac ccaatccatt gattgcttga
     4501 caaatctttt cggattgaac ttttgtttca tcaaacttga ccgtaccgtt tgcttttttc
     4561 aaatttactt tcactttttg cacgccgtca agtgcgctta ctgtttcttc tactcttgct
     4621 gcacaatgat tgcagctcat accttcgatt gaaaattgtt gtttcatttt ttcttcctcc
     4681 tattgatgtt gtttacattc acattgacct gggatacagt tacattcgat cgtatctact
     4741 ggttgctttt gttccagttg tttcaaaatc tcattgatat cttcttgagt caaagtggct
     4801 tcttctacta gatccgcaat cgtctctcca atcctttttg cacagatatg ggaaaaaaga
     4861 ttttccgtcg cactgcgaac tgtttccatc tccgagacag ctggatgata aatgaatttt
     4921 tttccttctt gttctgtcca gatcacttct ttttttacca aacgtcccag caaagttttg
     4981 atcgttgctg gtttccaatc catcgaatca gctaagatct gcgtgatttc ttgggctgtc
     5041 gtctgtccaa gtgtccaaat aacacgcatg atctcccact ctgaatcact gatctttact
     5101 ggcgttttga ctttttccat ttatttcaca cctttcgttt acaaatgtaa tcattagctt
     5161 atgaatacag attacgcttg taaacaaaaa ctgtcaatta tttttgctct ctattttcaa
     5221 tttagttaaa aattcttgtt tctctcgtct tgttttaagg atgatcagtt tatttcttgc
     5281 attagctact tggatcagct gctgtatttt cggttcattg ttttcttcaa aatgccagat
     5341 aaaagataaa aattctaagt attccctgtt tagtttctct gaaaatgatt caggcatatc
     5401 tggccgagat tttttgttta tctttctttt tagagaacgc caaactactc ggtagatggc
     5461 gagataacgc ggcactttaa gccaaatgat cttatctgct tctttaatcc tttcctctaa
     5521 tgtagaagta taatttcctt caacaatcca tgacggatta gataccataa acgattgctg
     5581 ttcttgcaaa aaccactttt ttgcctgaat actgtagtct gttgtatgcc ataaaaggtc
     5641 tagcggcagt agcggaaagt cggtgatttc tttgatcttc cttgcgaggg tcgattttcc
     5701 cgacccactt tgtccaatca cgatgatctt cattcttctc cctcacttct ctttggaatt
     5761 tggttcgatc ataccactta taacgccgca aaaaaacaag ggactgtgac aaaagtcgtg
     5821 tcaaagtccc ttgtttcgaa taaatggtgt tcaatcagct gaccttcagc attaactcaa
     5881 aaaacggcaa aatatgagga gctatttttt cgtttgttcc tctaatactc aggtcaaatc
     5941 gctgatttta caacctcttc cttaaacggt gttctcctgc caacacctcg gtcacaaggc
     6001 acaatagtca acaaacatga gaagttgttt tttgaaattg ctccttgtga ctcggagtta
     6061 aacggcaggc tcactacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata
     6121 agcccagcaa aacgaaaaat atgaggggct atttttgttt ggctgttctt attacttgat
     6181 gcaggacagc tttttcacaa cctctgattc acggtgttcc agaagtagct tctgcgaaaa
     6241 acaagccgaa acttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
     6301 gaagctgacc acttctgtca caacctcttg taacaagtat ttctatttta aggctgaacc
     6361 actgtttctg gttcagtgat ttctttgttt ttgtctcgac gagcaattgc tggcaagatc
     6421 atacctaata agatcaagac aattggtgtg atgatattcg tgaataattg gaaccaccat
     6481 gctttaggat cagcagtata atctactttt ggcaccattc ctaggataca tgcaaacgca
     6541 gtgaaggcaa agcaccaagc accaaagata aaaccgattt ttggattttt aacaaattta
     6601 tattcagaat tgaatttctt ataagcttta ttcaacatca tatatgccaa gaacacccat
     6661 aggtaacgca ttggcatgac aactgagttc aagttcgtca accattttac taattcattc
     6721 atatctttga taccaaagat cggcaacaaa ataatgattc ctactagtat acctgtcaga
     6781 agataaccat tcaccaatgt acctttacga ttacgtttac gtagccagtt tggtacaaat
     6841 tctggatccg catctgctaa caagattttt agtggtgcat caattgaaaa ggctaatgcg
     6901 gagatttgtc caatcatgtt tgtcaatgcg taaatgatca tcaatgagtt accgatacca
     6961 tagtaattac ctaaaatttg aaaagcactg tatgccccat ttgccatcaa gtctttagga
     7021 atattatcgc tagcaaacag catacccatt gcgactgagc ctaatacagc acaaatccct
     7081 accattgctg ctaagaagat catcccttta ggaaattcct ttgcaggatt tcttgtagag
     7141 ttgacataag gggatatttt ttccgctcct ccaactgcaa atactagcat cgaaacggtt
     7201 gtgaaataac tgaaatcaaa tgttggaata tatgttttca cttgattcat atgtggtgtg
     7261 gcaaattcca tcgttggatt gatgaacggt gccgttacag ctaaaagaat aaataagatc
     7321 gacatgacga acatcgctgt tccggccagt ccgccaatca ctttcaaagt agaaagtcct
     7381 tttgttgata aatataaaaa ggcaagaaat ataataagtg aaataactgc taccatttgt
     7441 actgacatcg tatttacgat atttccattt ccttggactg cccaaccgcc agcaatcagg
     7501 attgcttgag gcttttgtgc caaataagga atatggacga cccagtaagt ccaagcggca
     7561 tagtatgcca gtcgtttatt agatgtcgta ttttcgaccc atgaactgac gcctccttta
     7621 ctatccttga aggctgaccc taattgtcca acgatcaatg cgtatggaat aaaataaatg
     7681 gccaaaatca atagccaaga ggtgacaact gatactcctt gctgagcata gttattaact
     7741 acattcccca tgccccaaac catgttaaat gcaattaacg caacagtaaa ccagcgtaat
     7801 tgtttgttgt tcattcgttc tctcctttat gataaaaatt tgttatccag atgtaatcat
     7861 attcaatttt taacaaactt acaagacagt gttattttat gtgtgtattc ttttatttaa
     7921 tacacatcgt tttttcatat tttttccatc aaaaaaataa gttattttct tttatttcat
     7981 ttaccttatt gaatttcaag ctattttgaa ttttctgcaa aattaaaaac attagttatt
     8041 ttataatcct atctattttc tttacatttt tcattaagaa aaggatataa aaaaaagagg
     8101 acgttcattc gtcctcttca ttgatcaacg tttatttttt acatgcaagc ttcgcatggc
     8161 attcaaaaca gctaatactg tcaccccaac atctgcaaat actgcatctc ccattgatgc
     8221 aaaaccaaaa gcacctaagg caagtaccaa aatttttatc ccaatagcaa agacaatgtt
     8281 ctgtttgacg attttcaatg ttttctttgc taaatggatt gcttctgcaa tcttcgatgg
     8341 ttgatcattc atgatgacta catctgctgc ttcaattgca gcatcgcttc ccaatccacc
     8401 cattgcgatt cctacatctg ctcttgctag caccggtgca tcgttgattc catctccgac
     8461 aaatgcggtc tttttattgt ttctttgtaa gatttcctct aaccgctgca ctttatcttc
     8521 tggaagcaat tcgctatata tcttgtccac gcctacttgc ttcccgatat gatcagcaat
     8581 ttttttcgaa tcgcccgtca acatgactgt gttcttcaca ccagcctttt ttaattgaac
     8641 caatgcgtct acggcatctt ctttcaaggt atctgcaata acaagataac cactgtaact
     8701 ttgatcgatc gctagataaa gcaatgtacc gatttcttga cttgaaggaa aagagatttc
     8761 aaatctttcc atcaatttgt gattacctgc taatacttgt ttgccatcaa cttcagctaa
     8821 gatcccttca cctgcaaatt cttggatatt agctgttggt aaaagcggac ctgcgtagcc
     8881 ttctaaaaca gattgagcaa tcggatgtgt agaaaactgt tctgcactcg caacatattg
     8941 catgaatatc tttggatcga tttttgtatc gatcgtctgg atcgaaaagt cacctttcgt
     9001 taatgtgccg gttttatcga ataccactgt ctcagtttgt gcaagcaact ccaagtaatt
     9061 tgaacctttt actaatacac cgattttact tgctcctccg ataccgccaa agaaactaag
     9121 gggtacggag atcactaatg cacatggaca tgagatcact aaaaatgtca atgctcgata
     9181 gatccattca gaaaacgccg ctccagaaac aacaagtgga ggtaaaacag ctaataaaac
     9241 ggccaatcct acaacgactg gtgtgtaata gcgcgcgaat ttagtaatga aattttcagc
     9301 tggtgctttt ttactgctgg cattttctac taattctaat aatttgctga tagtagaatt
     9361 ttcataagat ttttctactt gaactaaaaa tggtttattt ttattgataa atccacttaa
     9421 tactgtttca ccaacatata cagatttagg gactgattct cctgtaagtg ctgacgtatc
     9481 gactaatgat tctccttcga tgatcgttcc atctaacggt acacgttcac caggcttcac
     9541 aagtacaaac tgaccgatct gcacgtcttc agggttcacg gtcatcaatc cttccgtcgt
     9601 ctgcacgtga gctgtttctg gacggatcgc catcaattcg cgaatcgatt ttcttgaatg
     9661 actgactgcc aatccttgga aatactcgcc gatttgataa aatagcatca ctgctacggc
     9721 ttctggatat tcaccgatca acatagcgcc aatcgtagca acagacataa gaaaattctc
     9781 atcaaagatt tttcctttga cgatattcat cactgccttc ttcactacat catatcctat
     9841 cagcagataa atcaataaat accctatcaa gcgaattggt tcattaaaat caaataatag
     9901 caataaagcg atacctgcaa gggaaaataa aatccggaac agcgtatttc tctctttttc
     9961 atttccatgt tcagatactt tttccttatc tatttctgtt actttgacat ctggttctaa
    10021 ttccttcact acttttttta tttcttgttc aatcgtagcc aaatgatctt cctctgcgtc
    10081 aatcgttagt tttccagaag tgaagtttac cgttgcttcc ttgactccat tgatcttctg
    10141 aactcctttt tcgattttca ttgcacagtt cgcacaatct aaaccttcta atctgtaact
    10201 tttcatttta atgacctcct cgcctcaaca tatgaatatc tcttcataac taattataaa
    10261 tgaacggtta ttcatatgtc aaggtatccg aaagaaaaaa tgaaaaacat ctgcatcgga
    10321 aaatttcttc catacagatg ttttggctta ttctttcttt ttcttttcga tcattgccac
    10381 tgctcttatc caacccgcac ttgccttctt gatcttgaca ggatagcaac taagtttgaa
    10441 gcctgttgct ggcaatttgt ctaaattcat taatttttcg ataggacaat atcttttttc
    10501 aatccctgca aaatgtcctt cccagatatt cgaagctcca ctacgctttc aaaaatatag
    10561 aaagagttct ttaatgaaaa ttatttcaac tttccataaa cagtaacccc atataaataa
    10621 aagacgtaaa agtcacatgg aagaacttat acaggagaac gcatagagtc aggtgaaata
    10681 catgtatccc acctgactct atgctttatc taacctttat tattctcttt tatacaattt
    10741 ctgcaccgaa tggcatcaac gccaatcgag caagtttgaa agattgcttg gcaaaaggta
    10801 tccctatgat cgtgatacat agaaggatgc cgctgacgac atgtgcgaga gcaattggta
    10861 ggcctgaaac gatcagccag atgatattaa caatcaatga catgccagac tggtgataaa
    10921 ttacttcttt gccaaaaggc gcaaaagaaa gggaggcgat cttaaaacat tgcataccga
    10981 ttgggatacc tatgatcgtg atacaccaaa gtatccccgc tgctaaccag ctaagaccac
    11041 cgattagtcc gccgaatata aaccagatga catttcctaa acaactcatt gtatttctcc
    11101 ttctattact gtttttgatt aaaactgggc aaaaaatgat tttattcttc tttttttagc
    11161 ttccagtatc aaatattctg caaatttttt ttggatttct tccgtatttt cttttgttac
    11221 taatgtgtgt tggattagtt ttcctgatgg tcgctgatcc aatcgttctt ctgactgttg
    11281 cacgtccgct tgcgcaaatc cgataatccg tttttctttt gttacagcaa cgagcaatag
    11341 tttattctta tcatttaggt cagctgccgt ttttgctttt acgaatgatt ctggatgatc
    11401 agtaatcagc tgattgattt gaggtagatc ttcttttact gcccaccgta tttccatcat
    11461 ttctcacttt tctttcgctc atttttatct ccactaaaaa aatcgactgc ggcaattgca
    11521 tctatccagc tttgtgcaat gatctcatgt ccgccaatcg ttgggtggac cccatcttct
    11581 cttgtatatt tcgaaaaacc ataaacaatc cctaaagcat tgaatatacc atctaaagga
    11641 accagcaatg cgccatattc cttagcaagt ttacgaacaa tttggatttt agggtctaag
    11701 tctttacgcc agccatgacg gtcgtcggtt ataggaagta caaacggctc catcaagatg
    11761 atgttgcaga ttcccactgt ttgaagttca tttagtaatt ttcgataaac ggattcaaat
    11821 tgctgagcag atttctcagt tccgaatata gcctgatcgc tagtattatg ccaggtatca
    11881 ttgattccga tcaaaatcga tacatagtca ggttgtaaag ctaaacaatc ttcttgccag
    11941 cgtacttgca gatctgcaat tttgtctcct cctatcccac ggttcatgca ttcaaattcc
    12001 atcgttggat attgggagat tagttttccg gcaatcaaaa gtggataacc tttacctaga
    12061 tcagttgctt ctgctcggtc tcttcctgcg tctgtaatgc tatcaccgat aaataatata
    12121 cgttttttca tttgtcaaaa actccttttt gaataaaacg atgattttct tccttatatt
    12181 atcccataag ccctatcaag acgaaagaga aaatatatgt ataaaaaatg acaccagcct
    12241 agtcccttca aacaattgaa tgaacgctca tcaagtgtct agttttttat gctatttttt
    12301 tcttaacgtg ccggattccc cttgattcct tcgttgtttg gtccagagag atcgatcaca
    12361 cctaagcttc cgtcaatcag accactgatg acgccatcag aacatatatt tataggctca
    12421 tctaagactt ctagtaaatg tccctttact aatatctctt ctagattgct tgggatagat
    12481 tggtcataac tttatctggt ccgatgattt caagggtgaa ttgaacttac atatggatta
    12541 ttgcatgctg aaaatacgga cctactccat cttcgaatgt tactattagt ttgttttcag
    12601 gtgctagatg ctgctttatt tcaacagcag aagcatcatc aaatcagatt tccatttgaa
    12661 ttaccttctt tgtaaatgtt tgattgattg cgatttcgtt atagaacctt gtgaatcaaa
    12721 tagcaatttc ttttgctttc ttctcttgtc tgctggaaac ttcctttatc ccgtatttta
    12781 aaaaaagaac tgcgagagtt cgcagttctt ttttagaata aacggtgctc tccggccaac
    12841 tcctcggtca caagtcacaa tagtcatcaa acatgaaaaa ctgttttttg aaattactcc
    12901 ttgtgactcg gagctgaacg gcaggcgcac aacctcttga tataaacggt gttcaatcag
    12961 ctgaccctcg atattagcta aaaaaacggt aaaatatgag aagctatttt tccgttttgt
    13021 ttctctaata ctcaggtcaa ggcactgatt tcacaacctc ttccttaaac ggtgttcaaa
    13081 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tggaaagcca tttccacaaa
    13141 atttttctta atactcgaaa ctaaacagct ttttcacaac ctctccacta aacggtattc
    13201 aaaagcttac atctgcggta ataagtcccg ccaattcaaa aacatgaaca gctgtttttg
    13261 cttggctgtt ctaattactt gatgcagaac agctttttca tcacctctta tggataaatt
    13321 cggttcaaca aacgtgggaa tggaatagct tcacgcacat ggtcgatacc agcaatccaa
    13381 gtgactgttc gttcaaggcc tagaccaaaa ccagagtgcg gaacagaacc gtatttacgt
    13441 agatcaagat accatgaata ttctttttca tccaaaccgt gattacggat ttgttctagc
    13501 aagaaatcat agtccgttgc ccgttcgctt ccgccaataa tttcaccgta accttctggt
    13561 gcgatcatat ctgcacaaat taccacgtct tctcttgttg gatgtggttt catgtagaat
    13621 ggcttgatag cttttggata gtttaggata aagacaggct gttcgaatga attcgcaatg
    13681 aacgtttcat gaggtgaacc aaagtcttcg ccccattcga tgtcatcaaa tccatttttc
    13741 ttcagtaatt cgatcgcatc atcataagta atacgaggga atggcaattt tgtataagtt
    13801 tttaagactt ctttgtctcg tcctagtaca tctaatgcgt attcgcaatg atctaaaaca
    13861 ttttgtacta agaatgctac atattgttcc tgtacttcta aactttcttc ttgatgagtg
    13921 aaagccattt ctggttcgat catccagaat tcaatcaaat gacgtcgtgt ttttgatttt
    13981 tcagcacgga aagtcggacc gaatgaaaag acttttccga aagccatagc agcagcttcc
    14041 atgtataatt ggccactttg agataaataa gctttttgat caaagtaatt tgtttcgaat
    14101 agatccgtag tgccttctgg tgcggaacca gtcaagatcg gcggatcgat tttgacaaaa
    14161 tgatgttgat taaagaattc atacgttgca cgaatgattt catttctgat ctgcataatc
    14221 gcatgttgtc ttgaagaacg tagccataaa tgacgatgat ccatcaagaa gtctgttccg
    14281 tgttcttttg gtgtgatcgg ataatcgtgg ctctcgctta ccacttccac atttgtaatt
    14341 ccgatttcat aaccaaattt tgaacggctg tcttcacgga tttctccagt tactaacacg
    14401 gatgtttctt ggtgcagatt tttagctgtt tggaaaactt cttccggcac ttcgcttttt
    14461 acaactacac cttggaaaaa ggctgttccg tctcttaatt gtaaaaatgc gatttttccg
    14521 cttgatcgct tatttgcgac ccaagcgccg attgttactg tttctcccac atggtttttc
    14581 gaatcgatga tttgaatttg ttccacgtat gactctcctt ttatattcct tgaatttctt
    14641 caggattaat tttgactttt actctcaata aattgcttga ttctgcgtac cgcttcttct
    14701 aacgtagcgc gatcagtggc ataactcatg cggacatttt ctggtgcccc aaagccttcg
    14761 ccagtcacaa gcgctacccc tgtttcttct aaaagatctt ctacccattg tgtgacattt
    14821 tcatatttac acatgcgtag cgtttctttt acattaggaa aaagataaaa agctccttgt
    14881 ggcttttcaa gagaaacacc cggcagttct gccacaagcg gatataacgt attcaggcgt
    14941 tcttcaaagg cgttccgcat ttcttccact gtatcttgct cgcctttcaa tgcttcaaca
    15001 gctgcatatt gactgactgc tgtcggattg ctagttgatt gggaagcaac agcgatcatt
    15061 ccattgatga tcacaggatt tcccaccgca taaccaatgc gccagcctgt catcgcatat
    15121 gttttagaca cgccgttgat gatgatggtc tgatttttga ttgcttctga aatcgttgca
    15181 attggagtaa actcattgcc attataaact aaacggccat aaatatcatc agcaactatc
    15241 aaaatatcat tttttactgc ccattcaccg attgcctgca gttcattctt gctataaatc
    15301 attccagtcg gattcgatgg agaattgatg attacggctt ttgttttttc tgaacgtgct
    15361 tgttctagct gctcaacagt aactttccag ctattggatt cttctccttt caaaaagaca
    15421 ggaagtcctt ctgccagttt tacttgctca ccgtaactga cccagtaagg cacaggaatg
    15481 atgacttcat cttgcgggtc aaggatcgtt tggaaaaggg tatacaaagc aaatttcgct
    15541 ccgtctgtta cgactgtttg tgaaggttca tagacaagtc catagttttt cttcagataa
    15601 tcaacgattg cctgtcgaag ctctgggatt cctgctgttg gtgtataata gctagctttc
    15661 ccattgcgga ttgcttcgat tgctgcttct tggatgtttt ctggtgtcgc aaaatccggt
    15721 tctcccacag tcaaactcag cacatctctg cctttggctt ttaatgcctt ggcctttgcc
    15781 gcagctgcca aagtgacaga cggttctaat cgttttgttc tttttgataa ctccatccca
    15841 ttcattcctt taccttttaa attcccttga ttgtcttgac ttcatctcca gattcaaagc
    15901 caatcaggta ataattcagt ttcccatttg tttcttttcc tactacttcc cacacaggct
    15961 gatcatcata catccctaaa gcagcttttt cgatttggat ttcaggatgg gccgcagcaa
    16021 ttgtctgttt tgcttcattt tcagtcagtc cctttgtttg atccaatact tttacttttt
    16081 caccagattt aggaatgata acggcgattt cttttccttt atcatttgta cctgtcagtg
    16141 aaaagtatgt cttctttcga gtaaaccaat aaaaatgatc ggcttcctca agatttgcat
    16201 atttctttgc gatttctact gcttctttct tcgcctgcac cgccggacga ctggaacgca
    16261 agtaaaaaat cgagctgatc acgatgaccg ctaataaaaa tacgatcaag ccaagtaaca
    16321 cgcgattaat cgttttttcc tttgtctgtt ttttcttcaa gggtcgtcgc tcctttatct
    16381 tcattccttt tcattatagc agattttaat tatgaaaaaa gacagctcct ctaacttcct
    16441 tcttcatttt tctttaagaa ttcatggata tcctgataga tttcctccat agctagctct
    16501 ttgatcggta cttttttcgg taatgcatgg atgatccgtg caccatattt tgcagtaact
    16561 agtcttcgat ccaaaataag caatacgcca cgatctgtct ccgtccttat caagcgtcct
    16621 aatgcttggc gtaacttcaa agctgcttta ggtacggatt cctggtaaaa aggattgatc
    16681 ccctctgatt ctaaataagc gttcctagct ttgaccatcg ggcgaagcgg attatcgaag
    16741 ggtaaacgtg tgactacaac gatctgcaaa gcatctccag gcaaatctac accttcccaa
    16801 aaactgtcag cgccaaacaa tacgcttttt tctgacaaaa tgaagcgttt caaaagcttc
    16861 tcccggcttc cgccaaaccc ttgagctagt atttctcgtc ctttttctag catagacaga
    16921 tgcattttct gatagacctt ctgcaaaaca tcatgagaag tgaataacac aagtattggc
    16981 cgatcttctt gttcgatcag acctgtaagt gctgtctcta aataatcgat atatgcttga
    17041 ttgctgactt gatggacggc aggtgcctct tttggcacat agatccttgc ctgtttctca
    17101 taatcataag gagaggcaat cactttaaga ggtgctgctt caatgccaag ccgcttagag
    17161 agatagtgtc gattagggcc gatcttcagc gttcctccaa tataaagaat acgatcatat
    17221 cgaggatacc aaacagtcga cggcaacata gccgcttgaa aatcatggag atgaaaaatc
    17281 atctgctgcg ctgtgttccc aggaacgaac caatggatca aatgatctcg ccagtcttcc
    17341 agccattgat ccatcagcaa gtactgcttg tctaattgat caaataagct atataaactt
    17401 gctaaatcga tccgcatctg gtttgtccag ctttcttgga taatctcaaa agtcagacgc
    17461 atttgtgact gtagctgctg gatctcctga taaaaaagct gcaggtgatc aatggctgtc
    17521 ccagattcca gtggcagctg atccaacatc tcttttgtcg tcatgacttc ttctggatac
    17581 tgtccgttag gttgcttgcc gctcactaaa ctcattaatc cttctgccat acaaatctgt
    17641 gcttcgcata gcgcagaaag ttcttctcga taaatcgata accagcgtaa ggcttcatgt
    17701 tctttcggta aatagctttg tattcggtca aacagctgat ttggttcttc taattgctgg
    17761 atttgtttat ggaaatagtt aatacttaat cgtttgtcat ttactttttc agcgatttcc
    17821 ggcaaatgat gtacttcatc gatcaacaaa aatggactag ctggaagcaa tggttctttt
    17881 cgctgtgttt cttgtattaa aaacgcatga ttgacaatca aaaaattact ttgcctgatt
    17941 ttcttttgca agaaacgaac aaagtcttgt tcgtaaaatg actgatttct tgctaaaaat
    18001 ccagtgcctc tgtgcgcgat atctgcaaag aatgaatggt tcaaatttgt taaatgtaat
    18061 tcatccaaat caccggtctt cgtttgcgtc agccaaacta agaccgccat ttgatacaaa
    18121 gcatattgtt tttgttctgg cggttccaca agtgtgcctt taaaacgctg taaatcaata
    18181 tagtggcgat agcttttgat aattacaggt tgtatgggct gatctagcaa ttgattcagc
    18241 aatggaatgt ctttttctat gatctgctgc tgcaaaacta gcgaaacggt actgatgaca
    18301 gcgggttttt caggtgtagc caaataactc aatggcagca gataaccgat tgttttaccc
    18361 attcctgtag ctgcttcgat catcaaatct tttgattcat cttttgtgaa atggtcgtag
    18421 accgcattca tcagtctgtt ctgttctttt cgatacatca gtgttttacc aaacattttt
    18481 tcttttgctt gtttttttct tggatatgtc cgctcgccat aatggacgta tatgaatagt
    18541 tccacttctt ttttctgcaa agctagccca tcgacgattt ctaatgaggg atcaagtggc
    18601 tctggttttt ctttcatttc ttcaatcaca tgataaatga aacgactcgt atccatacct
    18661 gtgtgtctac ttaatcgcgc aatctgctct aacgtgatga ttggcaattt tctcatccgt
    18721 tcttcgatca gcaaaagaag ttgacccgtc acttcggcat cactatctgc ctgatgagga
    18781 ttctcatgaa taaatccaag gctttctgac aaatcagcta agcgaaagct tggttccgtc
    18841 ggcaagaaaa tttgtgcaag ttctactgta tcgatgcctg gtatcttcaa actaggaagt
    18901 ccgcatcgca tgagttcatg gttcaaaaaa ttataatcaa aataaatatt atgcgcgaca
    18961 aatatggtgt ctgccaaaag attgtaaatc gtttgggcga catcttcgaa ataaggcgct
    19021 ttttggacgc gttgattcgt gatccctgtt aaatgctgga tttgttttga aatcttccgc
    19081 cctggatgaa tatcgatcga aaaacgtgaa gtgatccgac ctgattcaat catgacgcag
    19141 ccaaattgga tgatccgatc ttcttttgga ttcgtcccag tcgtttcgat atcaacgact
    19201 gcataagttc ttggtttcaa aatatcttcc tcttctcctg cctctaatgt tcttttgaaa
    19261 ttatactaca aagtgcagaa aaatactctc atcgcttttt aaagaaaata gacaaaaacg
    19321 cccagacacg caaatgtctg ggcgtttatt ttttagtata ggttaattaa gctttagtaa
    19381 cgtttgttgc ttgaggtccg cgttggcctt cttcaacatc gaaagttact gcttggcctt
    19441 cttctaaagt tttgaatccg tcgccttgga tagctgagaa atgaacgaat acatcgtttc
    19501 cgttttctgc agtgatgaat ccaaatcctt tttctgagtt aaaccatttt actgtacctg
    19561 tttccataat aaaaatcctc cttgtgtatc aacacatatt ttgcaattac ttgaatggtg
    19621 tatatctgga agatgtttat ttgaaacaag ctaccacaga ttaccgaaca aaaactactc
    19681 ctatagtata tcataccgtt tacaaaaaag ctagagtttt tcgacttttt ttattaaaaa
    19741 aagttttctc agcgattaca taaagtgaaa aaccgaactt tttttaaaat aaacgacata
    19801 ttgttcagtt ggtgtttttt tgctctgatg ttcttcttta aactaaaaaa attattcttt
    19861 ctgactactt atcggttctg tttcaacggt cgaggctggg gaagattctt tagtaggtct
    19921 gatgttctca tggtatttct gatagcctat gacagttacc agaagaacaa ggcaaaccaa
    19981 tacgatcaac gagattagtt tttctttttt gctcatatct atacctcata aataacagga
    20041 tgatgtccat catatttctg agtatgagta aaagcaaaag ctgcagctgt agaatgtaag
    20101 aaatgccagc aactttcata atcacaggtc ggacagtacc aaaagtaacc aaatcttggc
    20161 tgcaaagacc gttcttctgt ttcaggaatc gcagaatcaa gatctgttgt ctgtacataa
    20221 acatttttgt acagacgtgt gcctgtaata ggagaccgct gtgtttcttc agcaaaaact
    20281 ggctgacttg ttaaacttag ggatagaaac agcaaacacc ataacagtct ttttttcata
    20341 aagacctcct tatcgataag tttttcccaa ttaaaatcat atctcaagaa cgcccgttat
    20401 tcacattttg ttcataaatg atgttttttg tatcataatc gtcaaaaaga ggtcgtgacc
    20461 acgtcacaac ctctttttta aacggtgttc cagaagtagc ttcttcggaa ataagtcgaa
    20521 atttcacaaa aatttgagaa ataatttttg tcaatctctt cttatttctt gaagctgacc
    20581 acttctgtca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtattagct
    20641 caaaaaacgg aaaaatatgt gaaactattt ttccgtttgt ttctctaata ctcaggtcaa
    20701 aacactgatt tcacaacctc ttttaataac gaagaatcgc tgtcagactc ttttcatctg
    20761 gcgcatcttt ttgatccaaa ataaacactt tcccgccatt aatgatcaca tcatctgcta
    20821 atgcattcaa cacttgacgc cgatcatatt ctgtttcagg atcttcaccg aaaccatcag
    20881 ctaagtttga cgtagcgata aataaatgtg acacttttcc ttctttcgca gctggaacga
    20941 tatcaactaa ctgatcgatg aatttcttat ctaagagttt attgtattct tttgattctt
    21001 gagctgcgag ttcacttgac agcttcttcg ccccttcttc gatatctcta aaggatagct
    21061 gcgcaggaga agcagaaatc gcaacgtctg cacaataata gggattttta gcaatttttt
    21121 tgaacatcgt ctgattttct ggtaatgcaa ataaataaat cggccattgt tcaggatttt
    21181 cgaaagaatc cttcaagtac gtatcaacag cttggtaata gttgacccaa tcgatctcta
    21241 cttcttcatc ttttggattt acgccatgga aagttactcc atctcgggct ccgcctcctc
    21301 cattagaaga gaagttcaaa ccgccacctg tcagttcaga tcccaatgca gtaacgacat
    21361 caaccggtgc cccttctggt agagatactt cttttacttg tttgttgctg acctcataca
    21421 atttcatgga gtcgcggttc aatcccaata aataataaga atagttgaac tgcccatttt
    21481 tgataatagc cagtaaataa ggaagatcac ttacatagta ttgatcgtct accgggatac
    21541 tcaaacgatg gatataggtc tggtcagcag ttataatgac cgctacactc gtagttgcac
    21601 tacgccaaaa cgaaggatct cctaacaaat tttcaatttt ttcttggaac ggggaccagt
    21661 catgttcgga atattttttc tcaaaacgtt tctttgcttc tttcgcaaaa tttttgaatt
    21721 tgatctgatc tttttccaca tcttgatggg cgacatgcgt attcagcatg atcgtgacga
    21781 atggtccttt agcgccttct gaagtcaatt gtgtcaataa ttctttatcc ttttttgcca
    21841 taaacgtacc cctcctaaaa aaatcattcc tttgtttcta tctaaagtct accacaacac
    21901 tatttttcca tacaataaaa cgtatctaaa aaaataaaac ggtttctacg gttttaaatt
    21961 tagctatttt tttaagttat cttcataatt tcctcaatta tttagcccca gtcttcaact
    22021 attcttcatg aaatgctgat aaactaagaa aaagaataaa agaggtggat cttatgcatt
    22081 tgattatgat tttcagttta ttaagtatta tcagtttatt tgctttcagc gcttcccgct
    22141 tattagatgc ggaaacacag cctattcctg taaaagtacg taaaaacgac cgctaaactt
    22201 ccataacgcg cgttcttatc ctaaagtttt ttcagaaaca ttgaaactct ctcccctctc
    22261 tgataaaatt ttaatttgta gcgaaacaaa ttaattacaa ggagatcgga tatgtccata
    22321 aaaaaaacac gtctagtcct attattggct agtctcattc tttgccttgc ttcttttcta
    22381 ccagctgttg ctgtgcaagc tgaagatacg ttcaaagtaa atgcaaaagc agcttttgct
    22441 gtagatgcgc aaagcggtaa gattctttat gatcaagatg gagaaaaaac catggggatc
    22501 gcttcaatca caaaaatcat cggtctttac atcgtccttg atcaagtaaa agaaggaaaa
    22561 ctgtcatggg atgataaagt ttcgatttca gattatgcag aaaatttgag tatcacccca
    22621 gatttatcga atgttccttt gcataaagaa aacacctaca ctgtcaaaga attattcgac
    22681 tctgcaatca tccagtcagc caatgcttca atggtggcct tggcagaaaa aatctctggc
    22741 agcgaagcta aattcactga acgaatgaaa gaacaattga aagattgggg aatcaaagat
    22801 gcaacaatcg ttaatgcatc aggattgaac aactcttatc ttggcgaaaa ccgaccagaa
    22861 ggcactggag aaaacgacga aaatcaaatg tctgctcaag atgtggccat cgttgcccgc
    22921 catttgatct tggatttccc agaaatactg gatgtatcaa gtactacgac ccaaatgttt
    22981 ggcgaaaata cgcaatcacc agtggaaatg gtcaactgga actggatgct cccaggattc
    23041 gttaattaca aggaaggcgt ggacggatta aaaactggta cgactgagct tgcaggtgct
    23101 tgtttcgttg gtacgatcac taaaaacgat caacggatca tcactgtcgt attgaatgcg
    23161 gacggccatg cggaaaaccc gagtgcccgc ttcaatgaaa cagaccgttt gatggactac
    23221 tgctatgaca attggtcaga aaaagagcta ggaaaagcta acgcaagtat tcctagtcta
    23281 aaaaccattg atgtaaaaga cggaaaagaa tcaacagttc ctgttgtgct taaaagccct
    23341 gtaaaagtat gggtacgaaa cgatatggat actggcaaat taacgatcac gccaacactt
    23401 gacaagaagc aagtcactga caatgagatc caagcaccta tggataaagg tacaaaaatc
    23461 ggtaccgcta cgatcacctt ggcagacgat aaattaggct atttagaaga caacaatgca
    23521 ccttcaacag agatcattac ggataaaaca gtggaaaaag cgaatatatt cgtgattggc
    23581 tggcgacgcg tcgcagaatt tttcggcaac ttgttttaat tgaacaataa gaaaaacatc
    23641 tttccatcgg aaagatgttt ttttgctttt tctagtttaa ataacagctt ttttcatcta
    23701 ttttcgttat actgtataag aaaaaggagt tgaatttatg aatctaacca gagatctgat
    23761 ctttttagtt ctctatctta ttggggtatt ctctatcgtt tattggcgaa aacagaagaa
    23821 ggatccttcc ttgttctttt acggcagtac gtggacattt acggtaatct taacttattt
    23881 tgtcgtgttg gaaatttttt attcgacctt ttattttttg attccattgt tgttctttag
    23941 tatctttgct ttttcttatt tcacagaaaa acgaaggctg ctgaatggtc ttttattcaa
    24001 tgttttcctc atcagtttcg gcatctactt gtttgtcttg ctgtatgaaa cgcaaaatat
    24061 tttccttggc ggactgatcg ccttgatcac gattccattg ctacttgtcc ttttatttgg
    24121 tatttatgga ttgattgttt ttcttttttg gaacggggtc acagttttac gtagagaatc
    24181 gcattcatta gctaatcttt taacgttgat actagccatt tttcttacac tgtttcttgt
    24241 ttttgacttt ttcttgttaa aatatttgcc gcaatggata aatgctttgt ttttctgcgt
    24301 tcccctgatc ttgatctatc tatttatcgt attctacaac tttttgactg tctcattctt
    24361 gtatcagttc aaccggcctc gatacaatca agacttcatc gttgtgttag gcgctgggtt
    24421 gatcaatgga gaaacagttt ctcctcttct agccaagcga atcaataagg caattgcttt
    24481 ttatcgggct caaagccgtg ctacattaaa tccgcccatc ttattgatgt ctggcgggca
    24541 aggagcagac gaaaaagtac cagaggccat tgcgatgaaa caatatgcca tggaacaagg
    24601 aatccctgaa agagacattc ttgtcgaaac caattcgaca accactttgg agaacatgct
    24661 ctattcaaag gaaatcatgg atcagcaaat gaaagggcca taccgcgcga tctttagttc
    24721 caacaattac cacattttta gagccggtct ttatgcgtgg caagccaaat tgaaagcaaa
    24781 tggtattggt gcaaaaacag ctttctacta cttgcctaat gcgtttttaa gagaatacat
    24841 cgccatcttg atgatgaaca aaaaaagaca tatgatcatt tgcggattgc ttttcgcagg
    24901 ctccgcattc ttatcgctga ttactttact tttctaaaaa aacaaggtgt gacaaaacat
    24961 atgtcacacc ttgttttttt ctttcattct cttaactatt ggcaaaaaga gtatcccttt
    25021 tttgaccgct acttgctgag cagaataaat accgctgttg cctgaacaca taaagctgat
    25081 caagcagatc ataaagaagt atactgccgc ttgtgaaccg aacaattcga tccccatcac
    25141 aaagcaggca atcggtgtat ttgtcgcacc agaaaatact ccgataaagc ccaatcctgc
    25201 aagaaaagga acagaaacat ggagcaaagg cgctagtgct gcaccaagcg ttgcaccaat
    25261 ttcaaacaat ggtgtcactt ctcccccttg atatcccgcg cctaaagaca aaactgtgaa
    25321 gaataacttc ccaataaaat catagacatg tgcctgtcca ttaaatgatt cttgaagtaa
    25381 cggaagactc aatccgagat aacgttgtgt ttgcaaaatc aatgctgctg ccacaactac
    25441 agctccacct atcgtatttc gccaaacagg atgaggaatc cattgtgcat atcgcgcttt
    25501 taaaaagacg atcgaacggc tgaataccca accaatcaaa ccaaaagcaa tcccagcaac
    25561 aaaaactttg aagaacagca acatactcca ttcaggaatc acacccattt ggtaatgtag
    25621 atgagaaaca ccatagcttt ctgtcatgaa atttgcaaac agtgcagcaa agaagctagg
    25681 gaagatcgct tctgttcgga ctttcccgat tgccaacact tctaatccaa ataatgttcc
    25741 agctaaaggt gtgccaaata cagaactaaa acccgcactg attccactga taatcaagat
    25801 ttcccgttct gatttatcca atcggaacaa acgtgctgta ttatctgcta acgctccccc
    25861 catttggact gctgttcctt cacggccaac agatccccca aataaatggg tagttatggt
    25921 tccaaacagt gtaagtggaa taagtcgcaa cgggattttt tcttctccac catttccttg
    25981 atcgatcact aagttgtttc cacgactaga aagaccgccg tgatacgcat agagataagc
    26041 aaaaagcgca ccactaactg gtagtagata aaggagccaa ggatgtgata gtcggatatc
    26101 tgtcacaact gtcaggctat ttaagaaaaa agcagacaag ctccccatga atataccgag
    26161 aatcgctgtg atcaccagcc acttcagcat gtacactaaa atgcgtactg gactcggtaa
    26221 ttgtttttcc ataaaaaatt cctcctcaat aaaaaaacgc ctactaaa
//
LOCUS       DACDHL010000033        26161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDHL010000033 DACDHL010000000
VERSION     DACDHL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.033"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="IV228_001395"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="IV228_001395"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8717320.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="IV228_001396"
     CDS             1286..2461
                     /locus_tag="IV228_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8717321.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="IV228_001397"
     CDS             2465..3106
                     /locus_tag="IV228_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717322.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="IV228_001398"
     CDS             3103..4020
                     /locus_tag="IV228_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8717323.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="IV228_001399"
     CDS             4017..4688
                     /locus_tag="IV228_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717324.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="IV228_001400"
     CDS             complement(5229..5717)
                     /locus_tag="IV228_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8717325.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="IV228_001401"
     CDS             complement(5918..6184)
                     /locus_tag="IV228_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8717326.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="IV228_001402"
     CDS             complement(6184..6447)
                     /locus_tag="IV228_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8717327.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(6440..6712)
                     /locus_tag="IV228_001403"
     CDS             complement(6440..6712)
                     /locus_tag="IV228_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717328.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            6923..7108
                     /locus_tag="IV228_001404"
     CDS             6923..7108
                     /locus_tag="IV228_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8717329.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7250..9073)
                     /locus_tag="IV228_001405"
     CDS             complement(7250..9073)
                     /locus_tag="IV228_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8717330.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="IV228_001406"
     CDS             complement(9671..12376)
                     /locus_tag="IV228_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8717331.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="IV228_001407"
     CDS             complement(12682..13629)
                     /locus_tag="IV228_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8717332.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="IV228_001408"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="IV228_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8717333.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="IV228_001409"
     CDS             complement(14264..14626)
                     /locus_tag="IV228_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8717334.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /locus_tag="IV228_001410"
     CDS             complement(14637..15758)
                     /locus_tag="IV228_001410"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8717335.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /locus_tag="IV228_001411"
     CDS             complement(15774..16124)
                     /locus_tag="IV228_001411"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8717336.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="IV228_001412"
     CDS             complement(16254..17765)
                     /locus_tag="IV228_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8717337.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /locus_tag="IV228_001413"
     CDS             complement(18087..19439)
                     /locus_tag="IV228_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8717338.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="IV228_001414"
     CDS             complement(19592..20668)
                     /locus_tag="IV228_001414"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8717339.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="IV228_001415"
     CDS             20864..22609
                     /locus_tag="IV228_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8717340.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="IV228_001416"
     CDS             complement(22889..23326)
                     /locus_tag="IV228_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8717341.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="IV228_001417"
     CDS             complement(23310..24683)
                     /locus_tag="IV228_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717342.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="IV228_001418"
     CDS             complement(25005..25493)
                     /locus_tag="IV228_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8717343.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="IV228_001419"
     CDS             complement(25515..26117)
                     /locus_tag="IV228_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8717344.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7503 a         5561 c         4838 g         8259 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
    26161 t
//
LOCUS       DACDHL010000034        25260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDHL010000034 DACDHL010000000
VERSION     DACDHL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.034"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..84
                     /locus_tag="IV228_001420"
     tRNA            12..84
                     /locus_tag="IV228_001420"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="IV228_001421"
     tRNA            88..160
                     /locus_tag="IV228_001421"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="IV228_001422"
     tRNA            168..249
                     /locus_tag="IV228_001422"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="IV228_001423"
     tRNA            267..339
                     /locus_tag="IV228_001423"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="IV228_001424"
     tRNA            351..422
                     /locus_tag="IV228_001424"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="IV228_001425"
     tRNA            439..524
                     /locus_tag="IV228_001425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="IV228_001426"
     tRNA            535..608
                     /locus_tag="IV228_001426"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="IV228_001427"
     tRNA            618..691
                     /locus_tag="IV228_001427"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="IV228_001428"
     tRNA            711..783
                     /locus_tag="IV228_001428"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="IV228_001429"
     tRNA            811..884
                     /locus_tag="IV228_001429"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="IV228_001430"
     tRNA            898..971
                     /locus_tag="IV228_001430"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="IV228_001431"
     tRNA            995..1084
                     /locus_tag="IV228_001431"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="IV228_001432"
     tRNA            1097..1170
                     /locus_tag="IV228_001432"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="IV228_001433"
     tRNA            1189..1264
                     /locus_tag="IV228_001433"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="IV228_001434"
     tRNA            1266..1336
                     /locus_tag="IV228_001434"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="IV228_001435"
     tRNA            1350..1423
                     /locus_tag="IV228_001435"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="IV228_001436"
     tRNA            1448..1535
                     /locus_tag="IV228_001436"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="IV228_001437"
     tRNA            1557..1628
                     /locus_tag="IV228_001437"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="IV228_001438"
     tRNA            1651..1740
                     /locus_tag="IV228_001438"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="IV228_001439"
     tRNA            1753..1826
                     /locus_tag="IV228_001439"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="IV228_001440"
     tRNA            1845..1917
                     /locus_tag="IV228_001440"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="IV228_001441"
     tRNA            1921..1993
                     /locus_tag="IV228_001441"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="IV228_001442"
     tRNA            1998..2068
                     /locus_tag="IV228_001442"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="IV228_001443"
     tRNA            2081..2154
                     /locus_tag="IV228_001443"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="IV228_001444"
     tRNA            2166..2237
                     /locus_tag="IV228_001444"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     gene            2503..3696
                     /locus_tag="IV228_001445"
     CDS             2503..3696
                     /locus_tag="IV228_001445"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8717345.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="IV228_001446"
     CDS             3826..5298
                     /locus_tag="IV228_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8717346.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="IV228_001447"
     CDS             5381..6169
                     /locus_tag="IV228_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8717347.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="IV228_001448"
     CDS             complement(6236..6904)
                     /locus_tag="IV228_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8717348.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="IV228_001449"
     CDS             7019..8731
                     /locus_tag="IV228_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717349.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="IV228_001450"
     CDS             8734..10503
                     /locus_tag="IV228_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717350.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="IV228_001451"
     CDS             complement(10708..11274)
                     /locus_tag="IV228_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8717351.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="IV228_001452"
     CDS             complement(11274..12230)
                     /locus_tag="IV228_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8717352.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="IV228_001453"
     CDS             complement(12230..12886)
                     /locus_tag="IV228_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8717353.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="IV228_001454"
     CDS             13256..15274
                     /locus_tag="IV228_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8717354.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15701..18115
                     /locus_tag="IV228_001455"
     CDS             15701..18115
                     /locus_tag="IV228_001455"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8717355.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="IV228_001456"
     CDS             18463..20016
                     /locus_tag="IV228_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8717356.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="IV228_001457"
     CDS             complement(20397..22181)
                     /locus_tag="IV228_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717357.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="IV228_001458"
     CDS             complement(22196..23917)
                     /locus_tag="IV228_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717358.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="IV228_001459"
     CDS             24017..24778
                     /locus_tag="IV228_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8717359.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7809 a         4644 c         5374 g         7433 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtatct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
//
LOCUS       DACDHL010000035        24578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDHL010000035 DACDHL010000000
VERSION     DACDHL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.035"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(153..551)
                     /locus_tag="IV228_001460"
     CDS             complement(153..551)
                     /locus_tag="IV228_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8717360.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IV228_001461"
     CDS             complement(548..1321)
                     /locus_tag="IV228_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8717361.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IV228_001462"
     CDS             complement(1318..2001)
                     /locus_tag="IV228_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8717362.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IV228_001463"
     CDS             complement(2168..3055)
                     /locus_tag="IV228_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8717363.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IV228_001464"
     CDS             complement(3052..3921)
                     /locus_tag="IV228_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8717364.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="IV228_001465"
     CDS             complement(4058..5344)
                     /locus_tag="IV228_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8717365.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="IV228_001466"
     CDS             5617..6195
                     /locus_tag="IV228_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8717366.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLAHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7123
                     /locus_tag="IV228_001467"
                     /pseudo
     CDS             6195..7123
                     /locus_tag="IV228_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7120..8856
                     /locus_tag="IV228_001468"
     CDS             7120..8856
                     /locus_tag="IV228_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8717367.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IV228_001469"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IV228_001469"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8717368.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="IV228_001470"
     CDS             complement(9752..10261)
                     /locus_tag="IV228_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8717369.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IV228_001471"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IV228_001471"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8717370.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="IV228_001472"
     CDS             complement(11562..12653)
                     /locus_tag="IV228_001472"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8717371.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IV228_001473"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IV228_001473"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8717372.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IV228_001474"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IV228_001474"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8717373.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IV228_001475"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IV228_001475"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8717374.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="IV228_001476"
     CDS             complement(16616..17590)
                     /locus_tag="IV228_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8717375.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="IV228_001477"
     CDS             complement(17571..19454)
                     /locus_tag="IV228_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8717376.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="IV228_001478"
     CDS             complement(19472..21697)
                     /locus_tag="IV228_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8717377.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="IV228_001479"
     CDS             21812..22846
                     /locus_tag="IV228_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8717378.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="IV228_001480"
     CDS             complement(22913..23926)
                     /locus_tag="IV228_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8717379.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="IV228_001481"
     CDS             complement(24004..24192)
                     /locus_tag="IV228_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8717380.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7064 a         5341 c         4398 g         7775 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atgcaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc tttggcacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaaa
//
LOCUS       DACDHL010000036        24412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDHL010000036 DACDHL010000000
VERSION     DACDHL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.036"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..536
                     /locus_tag="IV228_001482"
     CDS             <1..536
                     /locus_tag="IV228_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8717381.1"
                     /translation="ATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYM
                     LTLSLLLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFN
                     TSILLVGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNR
                     KTVIRVDEEIKEQLTTK"
     gene            558..2573
                     /locus_tag="IV228_001483"
     CDS             558..2573
                     /locus_tag="IV228_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAP8717382.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(2656..4029)
                     /locus_tag="IV228_001484"
     CDS             complement(2656..4029)
                     /locus_tag="IV228_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8717383.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(4055..4339)
                     /locus_tag="IV228_001485"
     CDS             complement(4055..4339)
                     /locus_tag="IV228_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8717384.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(4341..6356)
                     /locus_tag="IV228_001486"
     CDS             complement(4341..6356)
                     /locus_tag="IV228_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8717385.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(6535..6840)
                     /locus_tag="IV228_001487"
     CDS             complement(6535..6840)
                     /locus_tag="IV228_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8717386.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(6929..7960)
                     /locus_tag="IV228_001488"
     CDS             complement(6929..7960)
                     /locus_tag="IV228_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8717387.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            8167..9042
                     /locus_tag="IV228_001489"
     CDS             8167..9042
                     /locus_tag="IV228_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8717388.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(9194..10036)
                     /locus_tag="IV228_001490"
     CDS             complement(9194..10036)
                     /locus_tag="IV228_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8717389.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKKQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(10128..12005)
                     /locus_tag="IV228_001491"
     CDS             complement(10128..12005)
                     /locus_tag="IV228_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8717390.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(12002..13423)
                     /locus_tag="IV228_001492"
     CDS             complement(12002..13423)
                     /locus_tag="IV228_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8717391.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(13426..16815)
                     /locus_tag="IV228_001493"
     CDS             complement(13426..16815)
                     /locus_tag="IV228_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8717392.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            17072..18463
                     /locus_tag="IV228_001494"
     CDS             17072..18463
                     /locus_tag="IV228_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8717393.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(18536..19183)
                     /locus_tag="IV228_001495"
     CDS             complement(18536..19183)
                     /locus_tag="IV228_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8717394.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(19191..19877)
                     /locus_tag="IV228_001496"
     CDS             complement(19191..19877)
                     /locus_tag="IV228_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717395.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(19883..20956)
                     /locus_tag="IV228_001497"
     CDS             complement(19883..20956)
                     /locus_tag="IV228_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8717396.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            21178..21852
                     /locus_tag="IV228_001498"
     CDS             21178..21852
                     /locus_tag="IV228_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8717397.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            21857..23197
                     /locus_tag="IV228_001499"
     CDS             21857..23197
                     /locus_tag="IV228_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8717398.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(23346..24332)
                     /locus_tag="IV228_001500"
     CDS             complement(23346..24332)
                     /locus_tag="IV228_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8717399.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7113 a         4610 c         4499 g         8190 t
ORIGIN      
        1 tagccacagt cctgacgact tattctctag gaaacgaaac taatgcagcg aatatcttaa
       61 caatcatgca attggcaagt atggctgccg gagtttgttt tggctggctt cagacaaaag
      121 cacatcgcta tatgcttaca ctttctttac tactgatggg aatcggcttt gtattgattg
      181 ccactgtgat aaacctgtat gtgattgggt ttggtgcgat cctaacagga atttcttttt
      241 cgttattcat tccttactta ttcaaccaag taagtatcca agcgcccaag tacgcggaga
      301 gtttcaatac ttcaatctta ttggttggcg ctaatttagg tactttcatt gcaccatatg
      361 gtttgctctt tctctctttt gcttctcttg ggagtaaaac acttgctgtt ttcgtcaatg
      421 gcagtatctt gctgttgatt agcgcgctta ttagccttct agtagtacac aggacgaata
      481 gaaagacagt gataagagta gatgaagaaa taaaagaaca attgacaaca aaataaattt
      541 gatggaggtc gtgtaaaatg acaaatgaaa aacataaaga tagatggctt tggtatcctg
      601 gagactttga gatccgccat ggactgctac aaaatttcca acgggaagaa cgaggttttg
      661 actggccagc gtactggtac atggatgatt gccatcggaa cgtcaagttt aagagatatt
      721 actttcttga tcaaccttcc atgtttaaag tgacgatcca aggagttggg tatgtagaaa
      781 ttaatggaca aaaacatcca tgtggaaaat ggctgacttg tccagctggt aaagcaaaaa
      841 ttcgtatctt tgtaggacat acatcagggc taccagcgat gtttatcgag ggagacgaag
      901 taaaatcaga tattggctgg acagcaagta attttattga agaatatcca gctggctgga
      961 gtccgcttta tgtcgatatc gcaaaagatc caaatcaaat ctattatcaa aaagaatgcg
     1021 tccatccagt cactgaaacg gagattggcg gaggaatctt attcgatttt gggcgagcag
     1081 tgaatggctg tgtagaacta atatctctgg atgaccatat cgaatctatc actctatgtt
     1141 atggagagtc cgatgtagaa gctatggata tcgattattg ctactataag caaataggaa
     1201 tgacaagaaa aatgaaagct cgtaaacgcg cttttcgcta tttgtttata ccagaggttt
     1261 ctactggaat ggttcaactt caagtttttc atgaattttt atctctttcg aaacaagctg
     1321 ctttttcaag cgattcttct ttactgaatc agatctggtc agtatcagaa gaaaccttca
     1381 agttatgtag tgatttgttt tttatcgacg gtatcaaacg agatcgctgg atttggtctg
     1441 gagatgcgta tcaatcattc atgatcaatc aatatctttt ttttgacgag gaaatcaata
     1501 aacgaacgat attagcatta agaggacaaa atgaaatcaa gcagcatatg aatactatcg
     1561 ttgactattc gatcttgtgg ttgattggta tcgaaaacca ctatatgatg acgaaagact
     1621 atgaattttt acgcatcgtc taccctaaga tgcaatcatt aatgagatac ttgatggagc
     1681 aaacgaatga attagggttt atttatggga gagcaaaaga ttggatattt attgattggt
     1741 ctgaaatgga taaagaagga acgcttgctg cagaacaagt cttattactg aaagcctatc
     1801 attccatgat cacttgcgga gatatcttag ctgaaacata tggagagaac gaaaattatc
     1861 atactcatct ctatcaagaa aaatatcaac tattgaaaca aaacctattt gactactttt
     1921 gggatgaaga acttggcgct tttatcgatt gctatgaatc tgggagaaga aatgtgacgc
     1981 gtcatgcgaa tattttcgct gttttattcg attttgctaa caaagaccaa cagcaatcga
     2041 tcttaaagaa tgtcttattg aatgacacta tcacacagat tactactcca tatttcaaat
     2101 tctatgaaca agatgcttta tgcaaactcg gacagacgaa tatcgtctac caaacgattc
     2161 ttgattattg gggagggatg ttatctcatg atgcggtaac tttctgggaa gaatacgacc
     2221 ctactcaaac aggaaatgaa aagtacagta tgtatggaga tccttatgga aaaagcttat
     2281 gccatgcatg gggagcaagc ccaatctatc ttttaggacg ctattttatc ggtttgtatc
     2341 caacaaagcc aggttatgaa gaatttatga tacaacctaa tctagattca tttgaaacac
     2401 tgaattgtac gctacctatc aaagatggaa agatttcgct tgaaaaaaca aaagataaga
     2461 tcgtgattac ttctgataga acaggcggaa cagtgaaaat gaatggtaaa agttatcctc
     2521 ttctagcaaa acagccactt gtcttaacaa gctattcaaa aataaatgca taaaacaaaa
     2581 aactgcttgc gacatatcag aagactttaa atcttcttct gatattgtcc aagcaatttt
     2641 tattttgaag tttgcttaat tctgttcttt agaaacaatg tttgttcttt ttccggcaaa
     2701 aagaaagcta gataatacga caagtaaaaa gaaagcgatg attccaactg tcaaggctat
     2761 ttgaccgtat tctcctagtt tcccaagtac aagtcctggt actagataat cagtatcggc
     2821 aaatgtagaa gaagcgattt taagatctcc taatactgga aggataaata atggcagcca
     2881 actaatcaat aagctattga aagcagaacc taataccgcc cctcttctac cgccagtcga
     2941 gttgccgaag acaccagaag cagctccgca gaagaaatgt cctactaccc caggaatgat
     3001 aactgtagtt tgcaagccta tcatagcaaa catggatagt gttcctacaa agaagctaac
     3061 aaaaaagcca attaatacag cattaggtgc atatggaaaa acgataggaa catctaatgc
     3121 tggtttagaa tcgggtacga gttttttagc aatcccttgg aatgctggaa cgatttctgc
     3181 caacaccatt cgaacccctt gaaggacgat aacaaaccca gctgtgaatg ttccagcttg
     3241 gatcagtgca aagattacag cgttgtctcc attgcttagt tctctttcaa caaatccagg
     3301 accagctaac aaagcaagtg cgacatatac gatcgacatc aagagcatga tcgatacagt
     3361 agaatctcgc aaaaagctga aacttttagg aatctttatg tcctctgtag aacggtcttt
     3421 gtttccaaaa aactttccga tccatccact agaagcataa ccgatatttc cggtatgccc
     3481 taacgcaact tgattgtttc cagtgatgtt cttcataaac ggttgacaaa gagcaggcgt
     3541 aatagttaat agtgttcctt cgaagacccc gcctaataag atcatccaca aatttgcagt
     3601 aaaccccgcg cttactaaaa tgactgctgt tagacaagaa acataaagca ttgcttgacc
     3661 tgtaagaaaa atgaatttat atcttgtgaa tctagcaagt aaaatattca cgatcatccc
     3721 tactagcatg atcaaagcag taggtgtacc gtattgttgc aaagcaatcg ccactactgc
     3781 ttcattgcta ggaactactc cttgagtatg cagagctgtt tggaacatcg aagcaaaagg
     3841 ggttagtgaa ttactgatta aaccagcacc gccgatcagt actaggaatc cgacaaatgt
     3901 tttgatagtt ccttggatca cttctgaaaa tttcttcttc tgaagaacga gaccaagcag
     3961 tgcaatcaaa gaaacgagga tggagggctg actgacgatt gacacaaaca aattcaacaa
     4021 tttatccatt gatagttcct cccttgaatc atttttattc ttttgacaag atcttcgtta
     4081 ctttttcttt tacttcttcg ctgtcaatga tcgattgcaa aggtacgagc ttttcatcag
     4141 gtatagaagt tagcgtatct ttcaaagaat gctcaatgaa gaaataatca gccatatcgc
     4201 tagatacact tccaacatcc gtatgactta cttcaaactc gtccacatct gctccaagat
     4261 ctgcaagtgc ttgtttgatg ttcatctcta ccataaaact tgttcctaat ccactttgac
     4321 atactgcaaa aattttcata tcattcgtct ccttttctta tgatcgctaa tatttcttct
     4381 tttgttgatg cgtgtaataa atcttgaaga ttttgtttat tagaaagtag ctttgttaaa
     4441 tttgccaaag cccgaagatg tgcctcatta tcgatagcag ctaaacagat gaacaaacta
     4501 atgggatggg ctggattatc aagcagatcc acaggttgtt gaagtttcag caaagacatc
     4561 cctacttcgt ttacgccatc ttctggacga gcatgaggta atgcgatgtg atcgccaata
     4621 tggataaacg caccatactg ctccacgcgc tcgatcatag ctttgatata actttcttct
     4681 attttatttt ctgataatag cggctgtgcg gcaaatgtga ttgcttcttg ccagtttttt
     4741 ggttgatccg ataattggat catttcttct gttaataatt cagatagcat aggacgttga
     4801 tcctccttct tttctaaaag tttgttcatt ttacgattta atacttgata gagcttttct
     4861 tcgtctacgc cttcttttaa ttcgatataa ggcagtagcg ccgacaacaa ttcttgggca
     4921 gaaggcattg aaaatcctgg tatcagccaa tctctttgta cttgattgat cagctgattt
     4981 ttttccaagt cagtcatgat tggatgcaga acatagagag actttttctt atcagtaagt
     5041 gtgagaggta cagtagaaaa aataatatcg taagaagttt cgtcaacctc gtgtaagcgg
     5101 tcagctgaat tagtctctgt aaaaaagatc atcgggaata attgctgaag ttcacttttt
     5161 aagatcaatg aagagctaat gccacttgga cagacgatca cggctcttat ttttctattt
     5221 ctttcttctg cgtccgcttt tctgatctct ccgccaaata gtatagtgaa aaaaccaatc
     5281 tcttcttcag ggatgctctt ccctattcgt ctttctaacg gaagtaatgc ttttcttgtc
     5341 atttcgtaga tggaagcata ttgatgctta atttgatcga tcatcacatt aggtaaataa
     5401 aaagaatagg aaatacgaaa ataggcaggg accaagtgat aaaagacatt catcaataat
     5461 tcacgaaacg aagcaaattc tacggccatc aggaattcca tgcgatggat gacttctgaa
     5521 gaacattcca gcaaaaaatc aagtgcaggc tcctggacat ctccttcaac gatcgatgct
     5581 aagcaaagag agaaaaatat aatctccgaa ttttgatgtt cggaatagcc aaggccagtc
     5641 aatacttgtc gactaatgtc agtgactgca agagaagcca acagttcagc atcttttacc
     5701 agttccatgg atgtttttcc aattcgaatg gttgtcaaac tcaaataata agctaattca
     5761 tccattcgat ttggtgcgaa tcgaagatct gatagtgaaa tcgttttatc tagcaaatct
     5821 ctaactttgg cgtaataatc aatggaaagg gttgcggtta aatacgtgag cccaaatctt
     5881 ccagcaagtg actgcatcaa acgagcagtg cgattttttg cttctcttct taatattgct
     5941 tctgacccta gcagcctaaa accagttttc ctagtatacg acagctgaca atcttccact
     6001 tctaattcat ttcttatctt tttgatatct gacaggactg tccctttaga aacctgaaga
     6061 aaatcttgaa aatgaaaaac agaaacagac gttgcagtcg aataaaccat caaataaaga
     6121 agatctcttc gctcttgttc ggtatacaca gcttcaaatc gagtaatatc caaaaatttt
     6181 tctgcccagt cgattgctcg gaatttcttt atacggatac gggtatcatc cacatattcg
     6241 gcaaaaggag ttaattgttt gatcgcctcg cttgtattta cttcgatgat ctctgctgct
     6301 ttttctagac tgatttctct tggtgttttc aggagataac taattttgta gttcacagcg
     6361 ctgttcacct cctacttata ttctagtatg gtagcgcttt taaatcgagg ggtgtttggt
     6421 acaacttaca gttggatttt ttttacgact gtaccgaacg atttctatcg aatggataca
     6481 gaaaaaaagc aggtgaccaa atgtctcctg cttttatgaa ttgatttcgt ccgatcaatg
     6541 atgatcatgt ttattttttg aggtcagttc ttcaaatttt tctttgacat cttcaaccgc
     6601 tttttcgata tctttttcaa agtgattggg ctcattaggc tttgtttcct cttctacaga
     6661 atttgaacca gataaagctg tttctttatt ttctgtttca ccagagtttg tgccagctaa
     6721 tgattcttct ttgtcttctg tttcaccaga attagttcct gctaaagcca cttcttcttc
     6781 tgttttaggg gccattgaac ctcttaatcg tttgtgtttt tcaccgtcat aatttgtcat
     6841 gttggttcct cctagtagtt ctatactttt tcatcgtaca ataagacagt ttatgcgtca
     6901 aaaaacaaac ccgattctta caaatatgtc atatttttcc ttctgattct tgaatgcttt
     6961 tgattccttc ttccaaaatt ttcatttgta agattgtttc ttcatctttg atgacttttt
     7021 ctgcgccatt aactaatgtc tcgtaaatcc cttcgtagac acggctgtaa tcaccaacct
     7081 cggaaattac tttttcttca tgataactcc cttgttcatc tatataggtc aatacaccat
     7141 aatgttcggg caaatcgata ccaaagtctt catgatcagg catgtaaaac attttcagat
     7201 gttcttcttg tcgatctttt gtttgtttca cgaacatccc tttttttccg taaacgacga
     7261 aacttggacg ttctttgatg cggaaataac tggattttat cgaaattttc atatttccat
     7321 agaaaaaatc cagatcgaag taatcattca ttcgtccttt ccctaaaagt tgacgtacat
     7381 catagctgat atcatctggt tcgccgaaat aagaaagaac ttgatctact gtatgacaag
     7441 catgtccata taaatagctg ttcgcagtag aaaattcatg cgtattttct ggtacttccg
     7501 gtctgaaata atcataatgc atttctactt ctaaaatatc ccctaatttt ccgctttcga
     7561 ttactttttg gactgtcaag aagtcagaat cgaaccgtct gttttggtaa cattggataa
     7621 atagaccttt ttcttttgct aatgcaaata actcttctgc ttcctttgac gtttctgcaa
     7681 aaggtttctc tactagtaca ttcttaccat gttcgagtgc catttttcca tattcataat
     7741 gaaattgact aggtgtagtg atgacaacta attggatctc aggatcatcc caaagatcag
     7801 acagctgatc agtatattcg atgtcttcga atgttttcca cactggttca gatggggaaa
     7861 aaattttttt gatattgatt ttgttcgtaa gttttcttga aaaaggcaaa tgatagcgat
     7921 ttgtactctt tccattgcca agataagcga ttgtaagcat gagtggtgcc tccttttatt
     7981 gatgttttca gtatatccta gaaaagcgca gaaagtattt ataatagcta ttctggtaaa
     8041 aaagttcaga aaaacacttg ttatcattcg gtttggtgaa attaatgttc aaagtaagga
     8101 taaaaatcaa aaagatagta gcggaaccaa gtgtttatca gtaagataga atttggaggt
     8161 cagatcatgc ttattgaaga aaaactaaca aaacaagagt tgtttaccac gacagagaaa
     8221 cggattgtcg attacatccg taggaatatc gaagctgcag tctacatgac gattgaagaa
     8281 ttagcaaaag ctacgtatac ttcccattca gctatcattc gtttatgcaa aaaaatggga
     8341 ttcagcggat ttaaagaatt ccgtttcgag ctggggcggg aagttcatca attgattgct
     8401 cagttcaatc aaacgattga tcctaatttt cctttttcaa gtgaagatag cactcagcaa
     8461 attgcaaaga aaatggcgga tttatcgatc cagtcaataa aaaaagcaca attgcagatt
     8521 gaacatgaag atttagatca aatcggtaaa gtattgaaca aggcacagcg aatctttctt
     8581 tttgcaaaag gtgattctca aatcacggca aggaaattcc agaataaaat ggtcaagttg
     8641 aataagtttt tgatcatggc tgaagaatac agtgattctt cttggaatgc agccaatttg
     8701 actagcgagg attgcgccat ttttattagc tacagcggtc gcatccatca ttatgaacga
     8761 ataatgacct atttgaagca tgtagaggcc cctaccttgc tgattaccgg aaatcagcat
     8821 tcagaaatgg ccaaacaggc tgatatgtgt cttgttatat cgcaggagga atatgatttt
     8881 gctaaagtag cgactttctc ctctcaaatt gccttcgatt acgtattaaa tacgttattc
     8941 tcagtgatat attctcaaaa ttttgaagag aatatccgca acttgaaaaa caaacaagaa
     9001 ttgatgcaaa aaggccttct taaagaatat cggaaaactt aagaaaagat cctcatttag
     9061 taataagaaa gatagtgttt gataggctct ctttgatatc atcataaaaa caggctgaag
     9121 ttttcttttt caaacctcag cctgtttgtt tacggaacga ttcgaataga tatattatag
     9181 aaatgaggaa tagctaactt ctgtttctta ttttgaacga ttgacgaaaa agatatataa
     9241 gaaggaggaa aatcagtata catgcagcta acaatccata caggaaaaat cgtttgttct
     9301 cggatacttt ctgtacttct ttttgaactt cttttgcatg ataaggaacc cggtgccctc
     9361 tgacaagtaa gcgatgactg ttgaccatat aaggtgtaca agttaggagt gtcacgaaat
     9421 cctgcccttt ttcgattctc agatcttttg tatcagtcgg ttctaccgtc ttgatctgat
     9481 cgacttgata agcgagtgtc cgtccattga tttcgatata gaaagaatca cctaaagtca
     9541 gtttcggtaa gtctgtgaac aactttgctt gaggcagtcc tcgatggctg gaaataacgg
     9601 catgtgtatt ttctcccccc gtaggataag acgttccttc caataatgag gcaccttttt
     9661 ctagcagcac agtattcgtt tcatcaaaaa ttggcaaatg gacattgatc tcagggatcg
     9721 tcaaaatacc gatcgtatgt ttctcaaaat aggttctatc ctttttcgca ggttcttttt
     9781 tcttagtaaa gggatctgca cctggatttc caccttcttt tgctaattgc tgttttttct
     9841 ttgtcatgtt ttcttggatt ttagccatga cttcttcatt ttctttgacc gcttgttgct
     9901 gataatgaga gatgatctgc tgatccagat aattattcag cgcgtcgcta acaaatggat
     9961 aagctagtat ccctattcct gagagaagca agaaaagcat cagcagatct agaagtttcc
    10021 ttttctttgg tttcatttgt gttctccttg tgatgactgt ttgataataa aagaaaaagg
    10081 agcatttgcg taatccgctg ctcctttttc caattcttgc aaaaagacta aatctgactg
    10141 tgcttgcgtc tagcaaagta cagtccagca atcagtagaa ggactactcc tgcaccgata
    10201 tacacataga ttcccttacc gcctgttgaa ggaagtgtac ctttgtgttt atttggtatt
    10261 ttttcaggag aaatcaactg tcctgctgtt acataagatt gttcatcgat agtaaatgct
    10321 acacggtttg ttaatggaac atatttttct ggcgctttcg tttcttccag atagtacgtg
    10381 ccatatttta gacctgtcac atcgattaaa ccgttacttg tggttgtaaa aaccgttgct
    10441 gattctttcg ccgataccca gctgacggct tttgtggatg ggtcgatcga taaatatttc
    10501 gctgtgtcac tatcttgatc acgaacgacg aattctgctc cagcaagtgt ttggtctgat
    10561 gtaacgtcac catctacttt aacaaatcgt ttgcccccag taacgacatc gactgacggt
    10621 ggtgtttgat catttgtatg cccgttatcg acatttgctt ggttagaaaa tcctttggtt
    10681 ggatctgctt tttcgttcaa atgcatatag taaacgaatt tcaatgtacc gccaggagtt
    10741 aatgaaggaa tataattcgg atcaactgaa accgtgaatc cgtctgtttg ctcagtgaca
    10801 gaataattta ctgggtcgat ttctttattt ccatcatata aggcataagc atacgttcct
    10861 gaagaatcat tatcaaatgt taaagcagcg tcatgagtat cgataagttt gaatgttgtg
    10921 tacttgtttt gcgttccttc tttatcagcg atacctaatg ggatattgac agaaatctca
    10981 tattgaattt tttcaccgat tgcgacatct tttccattca attgaggtgt tgttttatca
    11041 actttagacg tatcattttt gatggtcttt tctactggtg tttggctatt tgccaggatc
    11101 tcaaaaggcg tgattgtttg cttttcaatc atttccgcat tatcaggagc ttttacttct
    11161 tctaaattat attttccaac ttctaaatga ttaacgatca actggccttt ttcaatagat
    11221 gcctcggcaa agtcattgtt gccgatgtca taagaatgac cagtaatgaa atgtttggct
    11281 ttggtttgat cagttgtcca agtgtacaat ccatctgtga cactttggat gtattttttg
    11341 acgcttggtg ttccttcttc tttagaaata ataaattctg ctccatttag tgcttcgttt
    11401 tcggcagtac cgatttttgt aactttcaac gttccatcat tacagactgt atttttaggg
    11461 tagagatgga tagtatctag ttcttctgtc ccgtatttat aagagccatc tgcttgtttg
    11521 atcatctcat atacagggaa agctaaaacc atgtttgcgg cagctgacac tccgtctttt
    11581 ggttcttctt tgatcgtgta gactgcatct ttcccatttt gttttttagg taaagataaa
    11641 gtgacatttc catcagcatc tgtcgttcct gaagcaactg gtgtaccagg agtcaaagac
    11701 tggactgctt gttttgctgc atccacggac gctcctttat ctcgttgcgc ataaaattct
    11761 tgagtgacgt tataaactga aaatgaaata tcggctaatc cttggtattg atcgaattcg
    11821 ctcatttctt tcccgctgtt ttggattaaa ggatcgggta aatcagtcat tttcttttta
    11881 tgaaggatga cttgagctgc tgtctcctct gcaaacactt tttttgcgcc gaagctgttt
    11941 gtgagtaatg gtaaaataag gaaaaggact cctaacataa cgtttatttt tttatgattt
    12001 ttcatttttt cactcctgtt ctttttttga taaacaacca gattgcaaag cttgcgaata
    12061 ccataccaac aacgcctaaa agtgtatttt tcgtttcgtt tgtttttgga agaaatggac
    12121 ggctattttc ttttttattg actactttca atatactgtc cgcctcttta tcctcagaaa
    12181 gaaaagttga aatggtaaac ggaatttctg tttcacttaa aatataacca gaaggtgctt
    12241 cgatctcttt taatcgataa cttcctgttt tcaacccgga aattgaaaaa gcgccatttt
    12301 tatcggaaat caggtgaagc gcatctgatt cgttctttgt ccacttgtat ccgttggctg
    12361 tttgctggag gtaatttcct gctttgtttt taataaggaa tactgcgcca gctaatcgtt
    12421 tatctggttt atttgtctcg attttgacaa attgcttact ccctgtatat acagagactt
    12481 tctttgtact gacaccaaaa ccaaaatcca gtgttccgtt gttgttgatt tccttgttcg
    12541 cctgtgctgt actgcttagc tgcatctgat aagaaatggt cagttttttt ccagcatatt
    12601 tttccaacga agggatcgaa aataaaacat caaatccgtg aggtttcttg tgtatgacaa
    12661 agccttctgt cagcttttct ccattcgatg aaatcgttaa actattaggt aaaaacgtta
    12721 atgcttcatc cgcactatct gacaattcga acttttgata gtcaaggata tttacaggaa
    12781 ttgtcgtatg caaggaataa gtgatttttt caccaatcgt aaaatcattt ctaggctctt
    12841 ctaatacctt ctcaaaaggt ggatcagggt agtcgttttc ttcattttta ggatacaaat
    12901 ggatagttga aagtttttga ttgttttgtc catgtacagg aagaacaact accatattct
    12961 ctgccttttc tttgacgact tctggtactt tggattcaat gaaaagatac gctttatctc
    13021 ttttttcttg atctttagcg gccagagaaa aagaagcgat accatcttcg ttgttaagag
    13081 ttgtagttgt tgcttctgcg gtaaacatac cggaagacgc accgtttttt gcgatctctg
    13141 cttgtgcttc ttctaccgtt ttgccctttt cccgtagatg gtaaaaagaa tctgtgacat
    13201 cataaacttg gaatgtgaca ccatttaatc ctcgatacgt ttgtaataaa gctttttctt
    13261 gtccgtcatt tggatgattt ttcggcagtt gcccgtttgg gaaaagcaat ttgtgtaaag
    13321 taatctgaac catttctgtt tctgtttctg ttgctacctg agtaaaagct ggtttaaata
    13381 gtaacaagaa gagacacata ctaagccaac caagtttttt catatttagc tgccccctct
    13441 ttcttttttc aaaaaaagat accaaataga aaacgcacaa ccaatcatcc ctactagata
    13501 gattcctaaa cggccaattc ctcccgtttc aggtaatggt acttttgcct gattcgtgat
    13561 gtctaaagaa atctggttgt ttttggctcc tggtgacaga acagattcat gatcttgtcc
    13621 atccacttga attgacccat cttcgtgtat aactatagta actggctctt ttagaccttg
    13681 gtatccttct ggtgtaaaag tttcggtcag cgtataagtt ccaggtttta aattttcgaa
    13741 tagaaaggta tctgtttctt gtccatcttt tggcgattct aagtccattt ctggtccctg
    13801 cagtctgaat tttgctcctt ttaatgtctg tccgttgtct tcttttttgt gtaccgttag
    13861 atcaaacgct tttagtcgat tttgatgagt gagcgtccat acattatttg tcatctcggt
    13921 attttttccc gtgtaaatga tttttccata tttatcgatt tggaattcat aatttcccgg
    13981 cgaagtatca tatccggcag gaccagctgt ttcaacgact cgatatttac caggtgtgag
    14041 tgaatcaaaa ctggcaagac ctttttcatt cgttgtttgg ctgtcaattg gctgataagt
    14101 accatttgct tcttttttct gtagggaaaa tgctgctcct ttaagattta tctctttgtc
    14161 cgtcccttgc atcgcatact tacgtactgc tacaggaact tcaacaaaag gattttcaat
    14221 agtcaactgg atcgtatcat cggaagtagt gactgttttt ccatcaatgg ttaccgtacc
    14281 atcagaagcg atcttgattt cccaagtagt ctttttgctt aatccatgcc cttctggagc
    14341 ttttgtttct gtcagcgtat aggtcatttc tttttgcaat ttgacatttt ctggaagaga
    14401 ataggttccg tcgccatgat ctgttagtaa agtatcaata gaatctcctg ttaatttgaa
    14461 aacagcgcca ataagatcct tttcacctgt agaggaattt ttcgttattt tcaagtttaa
    14521 cggtgtgaat tgtttagtat ttttccatgt cattgcatct atttgttgag aatcgtatcc
    14581 tggtacaggt aattctttga ttgtttggta actgaatgct tgaccttgat tattgtattg
    14641 aggtagtgat aatatctctt gataactttc tccgctattt gcagataatt tgtcaatgtc
    14701 tgcacgttcc cacgtatttg ttgtatcttt agctggttta atgattcgaa tatatccgtt
    14761 tttccaagct gcagcatttg tcgtatgttc ccgttgaatc tcaaaagtaa cttgatctgg
    14821 acgatcagct agattgttgt caaattcttc ccataacttt ttgatgtgaa gactgactcc
    14881 gggagcttta gcagatggta taccgaattc agctaattca ttggtatcaa tgtttggctg
    14941 gaaagttgtc ctgccgttca tttgatacca gaaatttgga tggaagtcct cattttctgt
    15001 ttggattctc acttggtaat ggatttggat ttcttggtct tttcctaaat agatctgatt
    15061 gctcttgatg gtatttcctt ctatggaaat agatggagtg accgttgtag gacttgtccc
    15121 cacactcttg actgaaagtg taccaggctg ataaatgaat ggttctgcga ttggatcatt
    15181 tatttgtcca ttgcttacag ttgctgagat ctgtacagct tttttggcta ggtattcaga
    15241 gatatctgtt gcatgatcag ctgattcata gtaaagatcg cctttttcat ctgatgaaac
    15301 catttgtcgc atacgagact ctacttctgc ttttgagaga ccagctgccg gatcgctttg
    15361 aagttggatg ccaagaccat gtatttcgat tcctcgttct ttgagtgcca tcgcttctcc
    15421 gatggttgcg ataaacgtac tgtcgattct tctggataga ttgttttggt caggtgcatc
    15481 atagattctt gagattagag aagtatttcc cggccgatct tgcgtattag aaaactgagt
    15541 tccgtaataa ttgctgcttg attgtgcgtg tacccgctgt actttatagg aaaatgttgg
    15601 tacaccatcc gtcagcaaaa cgatcacttt tttatgtcca tttggaacgg atagcatgct
    15661 tcctgcatct cttagtgctt tttgtgtaaa agtaccacca tttgtccgtg aaggtgtaat
    15721 ggattttact tgatttttca ctgaatcaaa tgaacccatc tgtactgcac cgttactgta
    15781 gctatatcct tcgcttgagt agccgacata tcccatattg attttgtctg tgataccgct
    15841 atctgctaaa gtatcgacaa aacgatcgac accaatcttt acttcaccga tccgattatt
    15901 gtcgttcata cttcctgacc agtctacgac caatacgaga tcaagaggcg tgatatcttt
    15961 ttgtacattt ccacgagcat tcaaataaac atcaaacaat ccgggtgtac ttgtttcctt
    16021 tgcatacttt cgtatcgcat agtctgcttg attaccggtt cctccatatt cgatatacga
    16081 atgggtccga tcattaggat ctccattcca agaatttata ccatcccatt gatttgttcc
    16141 gtttttattg ccttgatgat caagaacatt tgtatttcct gtaggttgcc aactggcttt
    16201 aggataagtg ccctgatcat ctgttgtata ttctgtagga atcgtttgat tagatggcac
    16261 actcacagct ggcaaggata atgtaggcaa tgtaaatggg ctgacagaat tttcgttttc
    16321 ttttgatgat tcttcttcta tttcgtctgg ctttggtact gacacatctg tatctttctt
    16381 attttctgtc tcatcaataa tgtcatattc tttattatcg acgatcagct tgtttgcctg
    16441 gctgtggtaa agtgctggat cgattggaaa actcagatcg atagtttgtt tagtgtttga
    16501 tgagatttcc aacgtaattt tcccgctatt tacctgataa gcatagccat ctccttcttt
    16561 tctttccaca tcacttaagt aattttcgac tagaccaatt tctttttcta tcttttcagc
    16621 actggtgtta ttcgcattta gcttgatgtt cagtgctttt tttccagact gatccgaaat
    16681 gacttcagct gaaattgttt ctaaaactgt tggatcatct gtagtgtctg ctattgcaga
    16741 taatggcaca aagtttgata aactcaatat tatcagcatg aaaataactt tcagtttctt
    16801 ccctgtggtc gtcatttttc ccctcctttt tctttttttc tgttttgacg tgaatagtga
    16861 aaattattaa aataaacagg cgttacataa ttttatttat tttaatatga taattattat
    16921 acgaagggcg ctaaagggaa tcacaccaat tttcatacca attggtttca tttatgaagt
    16981 tgattccttc gattaatgag cgtttcattg aaagaaaagg gttgagggag aaaaacatat
    17041 gatcaggttg ttggaatgca actatagaaa gatggaaaaa atcattcgaa ttctgtccat
    17101 ttctgacaag tggatgacta aaaaagaact tgccaaacga attggtagtt cagaatctac
    17161 atttgtaagg tatatcgaag agatcaaaat ccgctgggga acggatgtaa cgattcaatc
    17221 ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta ccagcttatt taaatgttct
    17281 gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta aatgagatta ttgtcaatcc
    17341 gggtaaaacg attgagtatt attgtgaaaa aatttccatc agccggtctt cttttacacg
    17401 taagttgaaa caatgcaatc aaattttaga aagctattta ttaaaagtga tcgttgacca
    17461 aggttttcag ttaactagcc ttaaaagtga gctccagttg cgtatctttg ttaccttttt
    17521 ctttttaagt tactacggaa gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa
    17581 agaaattatg gtcaagaata attgtcagat gaatttgttt caggaagaaa gcccatatga
    17641 gcaatctttc tttattatgt attttgttgt gcatctcatg agagaatccc aaggaactcc
    17701 atttgaatgg aaagctggag actcttatgt acagttgata aatatcgata aaaaagactt
    17761 cttaatcttg aaaaatctct taggaaaatt gaagtacagc agctatataa aagtagtgaa
    17821 ctattttgtc aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga
    17881 gcagatacga aaaaatatgt cctactgttc gttttcatcc gctgattgca aagaaaaaaa
    17941 cgaagaatat gttgttcaat tattaacgca aatttatttt ttctcccgta ttttcccata
    18001 cgatacgact catttgacga tccgtgcaac ttactttgca cagcagatgc aacaccgcca
    18061 gatacgtcta tatgctggat ttcgtgcaga acttcaattt tattccactc tactgaatca
    18121 gaatctgatt tattatttat ctagctatat cttttggatg cttatcggta atcctgaaat
    18181 tcaccattat acttctgata aaaaaatact gattatcagc gacttaagtc ttcgacatag
    18241 tcaatatatt gaagaatata tcagtgatat tcttgctgtg cataaaattc actctgagac
    18301 aactgctatt actgaagatc agctgtcgaa atacaatctt ctcgagtatg atctgatcgt
    18361 tacgaatcaa cccattttga atgcccatgt tcctcatata ttgattgatg atagtgtgtc
    18421 attcgcaaat gaagaagaac tcatccgctt attcgagtta tgaataatca aaaaacagct
    18481 atgtataaac caccctgata aaaatttcag gacaaataca catagctgtt tctttttatt
    18541 gtattccttc agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg
    18601 tccaattatt tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata
    18661 ggaaactaac tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca
    18721 gccatttttc gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat
    18781 catttcgctg ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc
    18841 agcaccactc taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga
    18901 attttaagat atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat
    18961 cttccaaatc ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga
    19021 tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat
    19081 cgattctctg tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct
    19141 ctcctttatc tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc
    19201 tttttctctt cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga
    19261 atcatacgga catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta
    19321 actagtaatt tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct
    19381 gctaagatca agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct
    19441 ccaccagaaa ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc
    19501 aaggaatcga tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat
    19561 tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct
    19621 ttaaagcgta aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc
    19681 actatttctc cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta
    19741 cctgaaccag aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt
    19801 gctttcaaag ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc
    19861 gttaatattt ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta
    19921 tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata
    19981 gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca
    20041 aataagcaaa caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta
    20101 ctaaagattt agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag
    20161 cagtcttctg aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga
    20221 acaaaaagta tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt
    20281 caatgaatgt cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt
    20341 tagctgaatc ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct
    20401 gttgtccata cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg
    20461 tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt
    20521 tgatcgtatc tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat
    20581 tctttgtttt aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg
    20641 tgccgaaaac tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga
    20701 ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg
    20761 caaatacttg atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg
    20821 cttttttaaa ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg
    20881 caatcaagaa cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt
    20941 tgatagctag aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac
    21001 ttgttgaata gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca
    21061 ataaattgtc taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta
    21121 agccttattt atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg
    21181 attaaactat tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc
    21241 gctgcaatcg gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa
    21301 agtggagttt atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa
    21361 gtgttagcaa aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa
    21421 gatagtttgg aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa
    21481 cctttctatc gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt
    21541 ctctttgatg aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa
    21601 gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat
    21661 tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga
    21721 tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat
    21781 ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg
    21841 caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc
    21901 gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat
    21961 attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc
    22021 tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac
    22081 tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg
    22141 ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat
    22201 tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat
    22261 ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac
    22321 gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa
    22381 tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt
    22441 ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat
    22501 tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc
    22561 gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat
    22621 tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac
    22681 ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat
    22741 gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat
    22801 agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa
    22861 aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga
    22921 agataagatc attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa
    22981 tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga
    23041 agatcaatct cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca
    23101 ggtaatcgaa cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga
    23161 ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg
    23221 tgacaaaaat cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct
    23281 tcttcggaaa taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct
    23341 tacgcttacg attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc
    23401 aattcttctc ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga
    23461 atcccaactt cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt
    23521 gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt
    23581 actggtttgc gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta
    23641 tcgttgacca catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca
    23701 tctaattttt cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg
    23761 tcttcagtct tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt
    23821 cctggtgtca tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc
    23881 attggatctg ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca
    23941 gtatcttgtg tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg
    24001 atgacaaatt ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt
    24061 gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa
    24121 gaacggtctt cttcaccatc catccgattt tcatctgttt caacatctat accagacaat
    24181 ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt
    24241 tcataccctt cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag
    24301 cttccgatga ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa atagatttat
    24361 gattggtata ttgttactta taaatttatt ctataggaga cgattgtttt ct
//
LOCUS       DACDHL010000037        24257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDHL010000037 DACDHL010000000
VERSION     DACDHL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.037"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="IV228_001501"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="IV228_001501"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8717400.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2486)
                     /gene="purK"
                     /locus_tag="IV228_001502"
     CDS             complement(1353..2486)
                     /gene="purK"
                     /locus_tag="IV228_001502"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8717401.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="IV228_001503"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="IV228_001503"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8717402.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="IV228_001504"
     CDS             complement(3374..4681)
                     /locus_tag="IV228_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8717403.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="IV228_001505"
     CDS             complement(4684..5265)
                     /locus_tag="IV228_001505"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8717404.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="IV228_001506"
     CDS             complement(5837..7129)
                     /locus_tag="IV228_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8717405.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="IV228_001507"
     CDS             complement(7126..7749)
                     /locus_tag="IV228_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717406.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="IV228_001508"
     CDS             complement(7752..8588)
                     /locus_tag="IV228_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8717407.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="IV228_001509"
     CDS             complement(8605..9492)
                     /locus_tag="IV228_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8717408.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="IV228_001510"
     CDS             complement(9508..10791)
                     /locus_tag="IV228_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8717409.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="IV228_001511"
     CDS             complement(11474..12157)
                     /locus_tag="IV228_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8717410.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="IV228_001512"
     CDS             complement(12175..12792)
                     /locus_tag="IV228_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8717411.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="IV228_001513"
     CDS             complement(12802..13626)
                     /locus_tag="IV228_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8717412.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="IV228_001514"
     CDS             complement(13619..14179)
                     /locus_tag="IV228_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717413.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="IV228_001515"
     CDS             14353..15309
                     /locus_tag="IV228_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8717414.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="IV228_001516"
     CDS             complement(15614..16570)
                     /locus_tag="IV228_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8717415.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="IV228_001517"
     CDS             16674..17276
                     /locus_tag="IV228_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8717416.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="IV228_001518"
     CDS             complement(17364..18863)
                     /locus_tag="IV228_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8717417.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="IV228_001519"
     CDS             complement(18879..19403)
                     /locus_tag="IV228_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717418.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="IV228_001520"
     CDS             19701..20450
                     /locus_tag="IV228_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8717419.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="IV228_001521"
     CDS             20441..21226
                     /locus_tag="IV228_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8717420.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="IV228_001522"
     CDS             21195..21911
                     /locus_tag="IV228_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8717421.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="IV228_001523"
     CDS             complement(22013..23032)
                     /locus_tag="IV228_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8717422.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24109)
                     /locus_tag="IV228_001524"
     CDS             complement(23051..24109)
                     /locus_tag="IV228_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8717423.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5223 c         4225 g         7621 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactacc cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgat
//
LOCUS       DACDHL010000038        23916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDHL010000038 DACDHL010000000
VERSION     DACDHL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.038"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..207)
                     /locus_tag="IV228_001525"
     CDS             complement(<1..207)
                     /locus_tag="IV228_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717424.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNE"
     gene            complement(214..762)
                     /locus_tag="IV228_001526"
     CDS             complement(214..762)
                     /locus_tag="IV228_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8717425.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(807..1139)
                     /locus_tag="IV228_001527"
     CDS             complement(807..1139)
                     /locus_tag="IV228_001527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717426.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1153..1383)
                     /locus_tag="IV228_001528"
     CDS             complement(1153..1383)
                     /locus_tag="IV228_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717427.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1605..2147)
                     /locus_tag="IV228_001529"
     CDS             complement(1605..2147)
                     /locus_tag="IV228_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717428.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2168..3259)
                     /locus_tag="IV228_001530"
     CDS             complement(2168..3259)
                     /locus_tag="IV228_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8717429.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3267..5477)
                     /locus_tag="IV228_001531"
     CDS             complement(3267..5477)
                     /locus_tag="IV228_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717430.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5493..8021)
                     /locus_tag="IV228_001532"
     CDS             complement(5493..8021)
                     /locus_tag="IV228_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8717431.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8073..8483)
                     /locus_tag="IV228_001533"
     CDS             complement(8073..8483)
                     /locus_tag="IV228_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717432.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8496..8714)
                     /locus_tag="IV228_001534"
     CDS             complement(8496..8714)
                     /locus_tag="IV228_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717433.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8711..9703)
                     /locus_tag="IV228_001535"
     CDS             complement(8711..9703)
                     /locus_tag="IV228_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8717434.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9782..10693)
                     /locus_tag="IV228_001536"
     CDS             complement(9782..10693)
                     /locus_tag="IV228_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8717435.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10712..11056)
                     /locus_tag="IV228_001537"
     CDS             complement(10712..11056)
                     /locus_tag="IV228_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717436.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11076..11632)
                     /locus_tag="IV228_001538"
                     /pseudo
     CDS             complement(11076..11632)
                     /locus_tag="IV228_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11713..12213)
                     /locus_tag="IV228_001539"
     CDS             complement(11713..12213)
                     /locus_tag="IV228_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717437.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12214..12759)
                     /locus_tag="IV228_001540"
     CDS             complement(12214..12759)
                     /locus_tag="IV228_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717438.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12756..14012)
                     /locus_tag="IV228_001541"
     CDS             complement(12756..14012)
                     /locus_tag="IV228_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8717439.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14373..14684)
                     /locus_tag="IV228_001542"
     CDS             complement(14373..14684)
                     /locus_tag="IV228_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717440.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14707..16017)
                     /locus_tag="IV228_001543"
     CDS             complement(14707..16017)
                     /locus_tag="IV228_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8717441.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16631..17083)
                     /locus_tag="IV228_001544"
     CDS             complement(16631..17083)
                     /locus_tag="IV228_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717442.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17085..17450)
                     /locus_tag="IV228_001545"
     CDS             complement(17085..17450)
                     /locus_tag="IV228_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717443.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17549..21832)
                     /locus_tag="IV228_001546"
     CDS             complement(17549..21832)
                     /locus_tag="IV228_001546"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP8717444.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21979..22218)
                     /locus_tag="IV228_001547"
     CDS             complement(21979..22218)
                     /locus_tag="IV228_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717445.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22308..22580)
                     /locus_tag="IV228_001548"
     CDS             complement(22308..22580)
                     /locus_tag="IV228_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717446.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(22663..22914)
                     /locus_tag="IV228_001549"
     CDS             complement(22663..22914)
                     /locus_tag="IV228_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717447.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTLVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         7056 a         5171 c         3494 g         8195 t
ORIGIN      
        1 ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat cgacaaacag
       61 taaatgatat aatccatttc cacctccaag catattcaat actggtgaac atgccttaga
      121 aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt gtttaaaagc
      181 catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc tagcagcagc
      241 tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag cttttcctaa
      301 ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa aagcttttaa
      361 tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc caggaagaga
      421 attaatggct acacatccag catatcgttt ccaatcaact ttctccattc taggaacaat
      481 aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac tgattcgttc
      541 acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa aaccaactgt
      601 atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat caactgtatt
      661 cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta aacctaataa
      721 gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc ctccataaaa
      781 aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc ttttaagatt
      841 gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa atgatcccat
      901 atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg gttcattttt
      961 tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc atcttggatt
     1021 tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata ttcaatctta
     1081 gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc ttcagacatt
     1141 ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg aaccagtaat
     1201 tacactatct acaacgccat tccaatttac tctacatttt ccatttttca gatttgtcgg
     1261 tccatacgga tagccttttt gacattcaat gttcggatta taccagatag gattatacca
     1321 ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa atccaataca
     1381 catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa aatagaatag
     1441 ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt tctgtcctat
     1501 aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt ctgttttttt
     1561 ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata cgcactccgt
     1621 acataataaa tcttgatcgc atcaatcttt tgagcttctt gatctacttt caattgaagt
     1681 aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata ttgatccgga
     1741 ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg tacatgcggg
     1801 tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct cacagcttga
     1861 ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt taagaaattt
     1921 tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga accaatttga
     1981 ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc gattgtttct
     2041 tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca accagtaaga
     2101 cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt tctagtcact
     2161 gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg tacgtgcgtt
     2221 gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag aaatgttgtc
     2281 cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga gacaatgcac
     2341 cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt gggttcatga
     2401 ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc gcaaacatgt
     2461 gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc gtaccaccat
     2521 aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc gcattgcctt
     2581 tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa tgcttgatcg
     2641 caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct cctcgataat
     2701 acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga atcaataaac
     2761 aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc gccaccataa
     2821 agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa ccaccttcag
     2881 aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac caggtaatcc
     2941 caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat tcatagccta
     3001 attgagaaag aatcacactc ggtaataact gatactttac agctgcacca actgctgctt
     3061 gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc ggtaatcctt
     3121 cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca tgctcagaaa
     3181 gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa agaagacaac
     3241 ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt ttggactgtt
     3301 cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg cagtgtttta
     3361 ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga aaatgcaggt
     3421 tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg ccccggttgt
     3481 tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga gtattggttc
     3541 aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac ttggcgaatc
     3601 tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc agaaggcaac
     3661 ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct ttgattattc
     3721 ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct tactgaattc
     3781 atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt tttcgatttg
     3841 gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact ataaggcaag
     3901 gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc ttttaccgat
     3961 tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc agaagcgagt
     4021 ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc caaattttgt
     4081 ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg acttgctatg
     4141 tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt taataaatct
     4201 ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt tcctaaaaaa
     4261 ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa aaatgccacc
     4321 aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga ctttaaggta
     4381 ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa aggaatcaca
     4441 agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat cactacaagt
     4501 ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc tttataaaca
     4561 gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc ttctcctgca
     4621 gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc cttggctaga
     4681 aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt cccataattt
     4741 aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac tttttgaatg
     4801 gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc caattcatct
     4861 agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc atttaaccct
     4921 ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca aaccattgaa
     4981 aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt ccctctaaat
     5041 atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc aataggatcg
     5101 tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa ctcgttcatt
     5161 gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat caatttactt
     5221 aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc cccaaataac
     5281 attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc aatatgaaat
     5341 ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac caatccacca
     5401 acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt cttttcctta
     5461 tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt atcctgttct
     5521 tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat ttcttcaaac
     5581 aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca acactgtcct
     5641 ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg taaatgattc
     5701 aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa attccctttt
     5761 tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg attcatctgg
     5821 gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga attattaaat
     5881 acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt tccaaacatt
     5941 tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc atctgtatat
     6001 agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa aatcgttgtt
     6061 ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg taaaatcgaa
     6121 tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg ttccatttgt
     6181 tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt ttgaactaaa
     6241 acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc taacggactt
     6301 aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa acaaacaatt
     6361 ggatctaata caccataatt acggggattt gtagcatcta acgtgacata actcattttt
     6421 tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg aatgagtttc
     6481 gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac attcataaac
     6541 gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt ttttccagtt
     6601 actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat ggctagaaga
     6661 ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt tttttctcct
     6721 cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga ttccatacct
     6781 aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt tcggtgcttc
     6841 caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa ttctcgttga
     6901 tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac cgttgcacta
     6961 ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat ccaatcgacg
     7021 acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg aacagttaat
     7081 aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt tttgaaccgt
     7141 tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg tggatagtga
     7201 attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa tagatcacaa
     7261 tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact actccctaac
     7321 tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata cgttccttga
     7381 ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat tcctcgaata
     7441 tattgcagac gattgagata caacagctct ttttttacta agggacgaat agaaaagcta
     7501 taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa agtttccaaa
     7561 tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa ccagattaga
     7621 tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat tcctaaaata
     7681 aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc ttttgcataa
     7741 tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc aaacttcgtc
     7801 cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc tgctaaaagc
     7861 aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt gttttttgaa
     7921 attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt taacaaacaa
     7981 tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca cctcccaata
     8041 aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac atacttattc
     8101 acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata atacacacaa
     8161 tcaccattca tcatacgaat catataacaa aaccaatccc ataaataaaa atgcactttt
     8221 tttccatcag gttttagttt tgccagtgct aacacacata aataggggag tcctatataa
     8281 aaaacaactc ctgctggtgg aaaaaaagaa atgacccaac caataaatga acgtaaaaat
     8341 aaatattcaa agattacgac caacaaaaaa gttgctgcat tgactaatgc aactggaaat
     8401 ggaagacgcc aattgcttgt aatctgttgt acgcgataag gttccgcata aacaatcgaa
     8461 taatcataag gttcccaatc cacttcacta tcctcctaat ccataaataa tttttcaatg
     8521 attttaccaa acgcatcaaa tacacgtgtt ggatcattta agaagaaata aactgcggca
     8581 cctgctgcga tagatattaa tgcatttcct acttttgatt tcataaaatt tcgcaatccc
     8641 agataaacaa ccaccattat taaaaaatag ccaccttgcg ttaatataaa atctacaatt
     8701 cccttcagtg tcatttccgt tcttctcctt tcctatccaa ccaattatgt gttaattttt
     8761 caacataata ctgatttgtt tttttcgtaa tgacaatcgt catttgttct ttttgtaaaa
     8821 gtgacgtatc accatctcga aagacgaccg ttgcttttac aataaaaggt tttttcgttt
     8881 ctttataacg caatactttc atctctactt gatcaacttt agctgtttga ttgagtcctt
     8941 caggttcttt catcatgtat gacatatctt ttgcattgtt tttcgcatac ttatctaaga
     9001 aatcttccac aaatccttca acatctttcg cttccgacgc atcaaccgtt tcgtataatg
     9061 tgtcatcata agtaatcatt cgttgttttt ctgtattctt tggtagtgcc gtaaaataag
     9121 gataagcaat aatacgacag cccttgtttg aaatagaaaa gggaagattg agtacttgtg
     9181 tgattgtcac acttgtttct ttcttactct ctggattcat cgagcgaata tcataagtta
     9241 ctttataact agcagttgga atggtttcaa tttcttttaa tccaataaac gaagcagcag
     9301 ttaattttcg aacacctgaa acacttcctg gttcttgaat aaagttttgt gcataaaact
     9361 ctttttttaa tgtttcttga cgttgttgtt cttgttcatg attatcgaat gtattcatat
     9421 acaaagcaac aaaacgattc ataaaggatt caatttcagg tgtgtacgtt aattctgacc
     9481 gactttgtac ttctttcttt tgtaattgtt ggatttcatc tttcaaaaac gacacttgaa
     9541 ccttcgtttt catggattga aaaaatgcca agccccctga aaaacaaaga aataaaacca
     9601 aacaccaaat ccatttcact gctttttttg aatggatttt cttttgcttc ggtactttct
     9661 ctgtataaac tttttctttc cgccgtagtt gtcgtttttt cataagctac tccttctagt
     9721 atgtttttac tagtacgtac gattgacaac gataagcttg tttttctgaa aaatagtcca
     9781 ttcaattacc ctcattttct attccttttc tctttctttt atatccaaca aatccagcaa
     9841 tacttgtcaa cactcctcct agaacagaaa gtgcaacatg tatttcttca ccagtctttg
     9901 gtaagacttt tgtttttcca tctgctgttt tgatttcaat attgccactt ggaagtttct
     9961 ttacaggaat cgacaaatcc tttaccaaat tcccttcact atcttggatt aatggaatac
    10021 catcttttac accaacaatt gtttgatcac ctgctacttt taatggggtg gttggtgttg
    10081 gaatatgggt caattcctct acattactag tgacaatcgg tacactcatt ccttgagaag
    10141 tattcgtcgt aatttcaccg cttggggtta ccgatactgt gacttctttt gctggaacaa
    10201 taggtttatt ttcttttgga attgcttctg atttttcaac aggttgtttc ggttcttctt
    10261 tttgttcttc actggtagac gacatcgttg gctgttccgt acttgacgaa tcagtattct
    10321 tttcagaaga actggttgtt cctgattgtt ccggagttgt gactatactt gaatccgttg
    10381 ttgttgaact atgcacacct tcttcttcga tcgaatcagt cggtaaagtt gattcaggaa
    10441 actctggttc tggtgtcact ggctcttttt caggtttctc tgactgcaat tgttcaggtt
    10501 ctgctggttt ttccgttgtt cctccattta gctctgtctc aacatctggt aatccttccc
    10561 ctggcaatgt ttctacaatt tctgacttag gtgtttctac agtttcatct gcataaacca
    10621 atgaactccc taattgaaac gaaaataatg aaattcctag tgttgttacg actgctaatg
    10681 ttgttttttt catgattatt cctccaattt tttatgcaat tcgtgttttg ttcatagacg
    10741 ccgcactaat attatctgaa cgataaaaga actcccgtcc gttttcttct aacccaacat
    10801 atggattatt tttgaataat tgaatcaata gttcactggt gttgtatttt ggatagtttg
    10861 caaaaaaata atactgtgct tgtcgcccat ccgaaaattc aacaagtaat tcataacgat
    10921 accgcttcaa tccttttcgt tgttttaaag gatctctata ccaacttgtc catcgttcca
    10981 ttcgtggata gagatagaca cgatacacat agaacaatgc cataccacat accaccaaaa
    11041 ataataatcg ccacataaaa attccccctt ttcttctacc tttcttgaat catcgaacat
    11101 tcataccctc tatagagatt tgaaaaatcc atcatcaatt gttcatataa acgttgttgc
    11161 aaatagcgat tgtttcgaat acataattct tcatagccat caatcacttg atcttctttt
    11221 tcatagcatc ttgtagctct atgacgctca gcttgcaaga cataaccaaa ctctgcacca
    11281 tatcgttcta ctgttaataa ctgttgacta cgcaataatg caatataaca atcacaaaaa
    11341 tgttcaaccg acatttgatt tttctcacgt ttgtgaaata ggtattcttt agaaagcaca
    11401 ttattcgtta aacaatctga taaatagcga taatctggcg gtcggttatt ttcatctgtg
    11461 aatttttccc ataataaact gatttttatt cgttcagcgg ctagatagtc ttgttcgcgt
    11521 ggataaaatt catcaatcgc acaaagtttt ttatcacctg aataaacaaa tccatataat
    11581 gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac ataccatctt
    11641 ctccttccta aaaaaaataa aaaagcagaa accgatttgg cttctacttt tctttccttt
    11701 tatagctctt ttttacggaa gaatccgcaa atgacatttt ttcaaaaaat caatcggcac
    11761 aagttcacga aaacttggag caattttgga tagaatcact tccccatctt gtaaaataca
    11821 atcataaaaa tcatcagtta cttgatactt acgcttctta caataattga taaaatagta
    11881 gcgatcattt tctatttcca ttcgttgttc tactaagcgc atacgaacat catcatagaa
    11941 ataagccaca aatcgccctt caagttcatg tttttcaatg ggatactctt ctggtaacaa
    12001 tctatttttt cgtcgtttat acattgctgg ataccttaga agaagttcat ctaaacgtct
    12061 gtcagtatat ggtaactgac gaatcaacga ttctaatcgt tctttataca gatagaataa
    12121 ttggcgcaag caatagtgta ccgcctctgg attttctaat actgttggat cacgtgacca
    12181 atacgcacca acataaatca taattttttt catttagaca atcacaccag caatactaat
    12241 gaccgatcca caataggtag aacaagtggc atagcgacca atttcctcta attctatctc
    12301 aatctcttct aaagaaagat atgtctgttc ttctgttggc tggcgactca tttcccaagt
    12361 gacagtcacc aatgaaattt cttccataag aggtttttcg ctgctaagtg ctgccacaca
    12421 gttttcatat atcttcattt tatcgcagcc ttttttaacc gctaaaatcg caggtattgg
    12481 caatgaaaat cgataaaagc gaatgaattc tagccttcct ttccttcggc taaattccaa
    12541 ctcttcgagt tcttgttcgt atcccttacg aacttctaat aagcgtgcaa tttcttgcca
    12601 gttctctttt tcctgataac gtccagcttt taccgcttgt tcaatataag gaactcgaaa
    12661 acgaaaataa gacaaatcaa ccatcttttc atttaatgct tcgattgcag cctttaaatc
    12721 caacaggcgc agccattctg ttgtcgtata tgcattcatg caattttccc tcctgacgtt
    12781 cccatccatt ctagatagtt ttgttgctcg tcatttagtt cggcattatc aatcccttct
    12841 tgtacaaagt ctcttcctaa aatacgatct gcttcttgta acaactttaa tgtcggagct
    12901 acttgtttca aaaaccagcg ttctgaacga tctaatgaga atttttctgg ctttactgta
    12961 aaatccaatt cttccatcga acgaaataat tttgcccatc gtttatcaac aatccgtgca
    13021 tcactttcca gtctatccca aacaataagt tttgcattaa ttaaaccaac gccaatctct
    13081 cctaaacgtt tcggtgaatc acctaaaata aatgcttcta ctgcttgtgt tgctttctca
    13141 ttggctaacc gtaactcata tcgattataa ataccatatt tcgttaatgc ttctaattct
    13201 gaaatttttt ctcggttcgc aatttcaaaa cgtttctcat aaaaattaaa aaagagagga
    13261 ctctttcgcg atccaaaata caaagataat ccttcacttc gaaaatagcc atcatctaaa
    13321 aaacaaccgc caatattctg gaatctctta aagtccattt ctaataaacc tgctttttgt
    13381 ttttctagta attcaaagag atcgaaatgt tcttccgttt ccaatcccca aagttcatct
    13441 aacgcaatat cgaatcggct acattgcaca cgtgtataac cagcttccaa ctttccttga
    13501 tttttccaaa tggaatatag ccatgttcgc caatctgttt gttgttcacg catgacttcc
    13561 tcatattccc ggcaaccttg tcctgaaaat tgcacaagta cattttctgt tacagttcgt
    13621 tttgaatgcc agaacaccca gatattccca cgaacagaaa gacagtcaaa aagatacaag
    13681 cccgttggac gtgtttcaaa ttctgtcaga tccattcgta atacacgttg aatcactgtt
    13741 ttgtagtctg tcccaaaaaa ggaaagacgc aaataatcaa tcattgctcg aagatctatt
    13801 ccgcttttca tatacaatcg aaagcgttcc ttgattcgtt gttcaaaatc ctctgggatt
    13861 tttcttttcc cttgttccat ctttctaatg taatcttttg agcagcccat caattgcgca
    13921 aatcttgctt gtgatagcgg ctttttatct ccacttatat tatgaattgc acttaacttc
    13981 tttctgtagc tttttagctc aatcggtgtc attttctcgc tccttttata aaataacaaa
    14041 atcgattttt tttaaccctt tagaaacacc gatatatcaa ctagtccccc tcgttttttg
    14101 ggaaaagtag tacaactgct gaccttagat aatcacttga tacaaaaggg tttataaaac
    14161 ttttttctat gtgttatgtt atttctgccc cccatattat atatcggggg gctttacaca
    14221 aagaggccgt gcgcaagcgc acgacctctt tgtgagctgc acacgctgct agcgagtgcg
    14281 cattataggc ccatgcttcg cagccttaat gcgcaccgcc aggcagagtg cggctaccag
    14341 actacgtcta gagccctccc gatagataat aactacaaaa ttaaatgatc taattttgtt
    14401 atttcactat atttttcagg agaaaaaacg cgataaattg acaaagttaa atcctcttct
    14461 aaatctactc gataatgata caatcgagtc aatcgttcta tatctttttt cgttagcaat
    14521 acttgattaa taacttttcc tgtaaaaaca cgttctttgc gcaaaaagcg gtaatcagtt
    14581 acttgttcaa tacgattcaa ccaatcataa aacgttctgg aagaaagaga aggattaatc
    14641 attcgtattg ctgtagctac tctataataa caactctctg ccatttaact tcacctcctt
    14701 agttgattac aaaatatgcg catcagaaaa acttgttcct tttggatctt ttgctaaata
    14761 catttggcgc tctagttgtt cttgtaacaa ctgattatca tctcccatcg tttgatcttg
    14821 actatcctct tttactgtaa aatcatagac aatcatttca gtaaatttct caaattcttt
    14881 gataaagtga taatctttgt aaacaaatgg cgcaaaaaac tctacaggta catcaaatcc
    14941 tggaaaagca tatccacgac caggttgatc tttgttgtaa aatggcttat ttttaaaatc
    15001 ttctccaaaa gtcattaaat aaccaatggg agacaatcga ccaacagaaa tacggaatgt
    15061 taaattatcc cgaacaccac ttggaaaata ttctgcatct ggtcgttgca ttgctaaaat
    15121 aatgtacatc cctgcttgac gaccctttaa tactaatggc tgaatcaatt tatcgactct
    15181 atttctcgct tccatatcca acgaaccata aaaagctgcc cattcatcaa aaaagataaa
    15241 atgcggtggt aacccataat aagcataatt tttccctggt tcatatttag gatctttcat
    15301 ggttttcata taggcatacc gttgattcat ttcttttaat gcatttcgta aacaaataat
    15361 catttcttca tttattttgt aaaacacatg tcctttgaat acatcgacgt cacctaatgc
    15421 tgctaaatct gccgcttttc catcgcaaat atctactgtc ccaacactta aacaagcttg
    15481 aattaaagaa tacaataaat aggttttccc accaccgatt gcaccagcaa tcaacatatt
    15541 tggtgattga tcatataccc aatccaaagt tttcattaaa tgaatacgtc cattttctgc
    15601 tatacattga tcaataggaa tccgaccaga atctgaaagg aatacataat tgattcgggc
    15661 tttttctcgt tcttcacgaa ttaaatcacg caaaaatact tcagataaca aatgacccat
    15721 ttttaaaaat cgttcgtgaa aacgcccacc atctaaagga aaagaaacca ctaattcttg
    15781 tttcttttga taaaaataaa cttttggtaa tctcattttt tcccttgttt gtttctcact
    15841 aacttgtttc tttgtataaa caaaccgatt gtcaattaac attcgaacaa gtaactgacg
    15901 ccaataaaca gaggcaaaat atccttcttt tagtcgcgac caatgcatca accctaaaat
    15961 taacccacta accagcaaca aatacaacac aatacttatt ccaggaataa gatacatttg
    16021 ccaattcgaa aacgtactta aacgtggata aaatactttc caactaaacc aacctaacag
    16081 aatcaaaaat ggaaacaaaa agaaaccata atagcaaaac cataaataac gattgaaata
    16141 gcgaattcgc cgaccacgat acttgaaaaa atgagctttc aattgtcaca ttcctccttt
    16201 ctgtttttac ttttcctgtg ctaaaaactt ttgacaaaaa gcaactaata caggaatatc
    16261 acatttagta aaaaaacata atcgaaaaag atttaatcga tttttataag ggtgtgcaca
    16321 catattacat attcgtacta tacgaatcca aatcacttcc tgatgatctt gtttccactg
    16381 gtacggtaat gaaaaacgaa caagatcacg taaaaacata cactgcccaa taaccgcaca
    16441 aatatatgca tttgtttgta caaaaatcgt tggattgtct ccaaaatact caatcaattg
    16501 ttggatattt tgataacaag cacaatctct caaagtattt gattggtttt atctgaattc
    16561 aaaatctccc cttccttcta tcttagataa aaagaatagg aagactcctt cctattcttt
    16621 caaaaaaacc ttattttttc tctttttcct tagaatcaac acttggttgt ttttgatcta
    16681 aacctttttc ttgtgattgt ttcatattag gattatttgt tggagcttct actttctcta
    16741 atcctttcgc ataaatcact aaatctgaat attcacgatt gccatcttgt gctggaatat
    16801 ataccacatg atattctgga tcaattaatt tcacaggatc acgtcgttta aaaccagtca
    16861 aatctacatc tgctggaaat ttaactcgtc gtgcaatctt tggttcatct ggatttccaa
    16921 aaactaattg tgctcgctgt gatcgaattt ctcccgtata atcgcctgtt tcattatcaa
    16981 atacacgatc agactcttct aatgcgccaa agcgtacggc tcctaaacaa gtttcatcta
    17041 atgcttctgt aatgtcataa ttatttggaa attttaatcc catcttacaa aacctcccca
    17101 atttttacac cagttaaaaa gacaccaaca tcttgatagc cagttgttcg acgctctgtt
    17161 tgtcccatat catttgtttc acgactattc actcgttctt tacgaaaaac aaaataagct
    17221 tcttcaaatg aaatacgcac ctctttttct ggtaatcgta atcttttatc ctcttcagct
    17281 gtgagataaa caaaaaaagc aggctgttct tctcgcgaac actctgcttg taattgaaca
    17341 tatcgcccag acacaccatt aatttccttt ggtggattag gttcccgtga acccaataaa
    17401 aatacttgac ctaaaaactc tttcttcata cgcaatgctt tttctgccat aaagtttttc
    17461 ctcctcttaa aaaccaaaat aaattctaaa tatttctaca ttacaatcaa acaagcggtt
    17521 aaaaatccta accgcttgtt cctcatgact aatctttggc tttacgtact ttataaagtg
    17581 ttatacctaa agcaatgact aatactacta atccaagaac cataagtata ctacttgttt
    17641 gttcacctgt ttttggcaac attggtacaa cagtagctgg tttaacccgt tgttctggtt
    17701 tcactggttg cggtggtttt ggcgcttcca caatttgaac cgtttgatct ttgtttgtta
    17761 gatctacatt gtgtttcgcc acttcttcaa acgtttcttt ctcatcttcc gttttcgctt
    17821 ggagccattc cgcaaaactc aagccttttc ctgcaagatt gcgaccgtcc actaagaatt
    17881 caaataagaa ttccatcatt tctttatcgg ccgcaaagac tttttccgca tgagtgcctt
    17941 caaatggtgt gccgtctggt gttaagtagc cttgaacaat taagcgatag atttgaccaa
    18001 tcgcaagacc gtctactttg acccattcaa gcacagtttg atttggcaag gccaagatct
    18061 cttttccgcc atttttattc gtcgctttcg tcgtaatggt tggattactg attttgatgg
    18121 tttggtcatt gtcggtaata tctgcgtgag aagccaataa tacgccttct ctaaacagat
    18181 cttcaaagac cacgacttct ttgcctcgca acgcacgtgc gtctagaata aagtctagat
    18241 tgactgtacc atccgttgtt tctgccacaa acgttaaggt acttgtgact tcttttccat
    18301 ctactagcac tggtttttcc gttactttgt ccattagaac accgttgact gtgtatgttt
    18361 ttccaacgac caaatcgtgg tatttcacag cgtctgtaat ggtgactttc gttaatggat
    18421 taacctcttt caagccagtg ggtttatgtg tagccgttgt acgtacactt gggttagtta
    18481 ctcgtaccgt ttgccctttg tcgttgatgt ctttatggtt tgctagttct tcatcatttc
    18541 taaaaagact ttcaaacacg acgatttcac gaccatttat acctgtcaaa tcaaaggtaa
    18601 atggtacgat caccgtttca ttttctgctt tggcaataaa gccaacactc gtttctaccg
    18661 tatttccatg cacaagcaag ggttcaccgg ttgctttatc cattaagatc cctttgacca
    18721 agtatgcttt tcctggaaga atctcggtaa tttcaacggt atcgtttaag gtaaacaatc
    18781 ctgttgggtc tgcgactgtc aatccgtctt caaagacagc cgtggtatga acggttggat
    18841 tattcacttt cacactttgg gcttcatcgt tgatatccgc atgtgctgca acttcaatgc
    18901 catcacggaa aagtttttca aaaacaacga tcgtttgacc tttcagaaca ctagcattaa
    18961 ccacaaattc aaggtctttt gtgccattga ctttgtctgc aataaaggcc aaggtacttg
    19021 tgatttcttt gccatcttgt aaaattggcg cattcgttgc cttgttcatc aatactcctg
    19081 aaatggtgta ttcttttccg acaaacaagt tggtataaga aacactgtct ttaatgacga
    19141 tcgtttctaa tggatcaacc actttttcac catcggcttt atttgacgct tgtgtttcta
    19201 gttttggtgg aatcaagtaa ttacgtacag aaaccactaa cgtttcccca tcatctggcg
    19261 cattttgttt gaatgcaacc gtgaatgctt gtttatctgt atttggttgg aaactttcta
    19321 atggggcatt ttccaagaag taatagtcat cggcaactag acctttcact aaaatttccc
    19381 cgttttgatc agtggtatat tcaccaattg cttgattttt accgttatac aaggtaaagt
    19441 tcacacctgc gagcggcgtt ttcttcttgt tccattcttc atacttgaat aattttacac
    19501 ttttccgtgc ttgacggttc gcaaaatgga tcactttgat cggttctgta tcattggtaa
    19561 tcgagaacac taatgggttt tgattcaaaa cgtaatttac gggggctttg atttcttcca
    19621 aacgatagcg atcttttcca taagtaagtg taccgttagt taggaaaaag cctttttcat
    19681 tggttttaaa gatatccgtt gtttctgtat catttggaat ctccattgtg acataacgat
    19741 tggctaaagt atcaaaaatc ttgaattctg tattagcaag tggaatcact tttcctgttt
    19801 cttgatccac tttcaccact ttaagacgtt gttcaattac tttatcttct aaaacataat
    19861 ggaaagtttg cccattttca gaaattgtga cttcaagtgg ttcaaatagt ttataccctt
    19921 cttttccttt ggtttcagaa agaatgtacg tatcaaagac aacggtattt ttaccaaaag
    19981 tagcataacc atcattgtct gtcacaacgg ttttcaccac tttgccagtt gtttttgacg
    20041 tcaaagtgat ttcaatgcca gctaattttg gtttgttatc tggattcgtt gaatcgacta
    20101 aatctttatt agcaactttc actaaatcaa tggttccttt gatcacttga tcttttgcgg
    20161 tcgcattgac agtttttcca ggttcaacaa caatttcaat tgcttcatca gaaagcacat
    20221 aaccttttgg cgctttgatt tcttttaaaa catatttgcc aggatcaaga tctttaattt
    20281 ctgcctgtac tttgccgtta atttttttca aggtaatttc tttaatcttt ttcccatctg
    20341 cttgatacaa accatagacc gcattttcta aagaagctgc accttgtgct ttcacaccag
    20401 tttccgcatc ttctttaaac acacgaatgt ttccttgttt tagaatttgt aaattaataa
    20461 cagacgcatt aggttctgtt ggatgtaaca caccaattgt ttgagctcct ggacgacgat
    20521 aggctaatgg cgcaccttga actttaattt tattcaattg aatttttgct ttatccggtg
    20581 cctcttttgt gactgtaatc catacttcat tgccgtgaat agatacattg acaccttttg
    20641 gtttttgaat gtctttcaca tatttcaaaa aatcattttt attctttact ttcaacgttt
    20701 cgccaatttt taccgtatgt gtttgattat taaaagttgc tttttcacca aagttattaa
    20761 tcaaacgttg aatttctgct tttcgacgat cataatcaac acggatattt ttgattggga
    20821 tttttgtgcc caaagcttcc caaatcataa attgtgccgc aatgtattgt tcatcggatt
    20881 tgtctttatt tgtgacatat cctaaataag agatcaagct tgtttttttc cgtacatttg
    20941 aatttttcaa atagtttttc aaagcagtag ctgtataaac ttgaccttct gcggctgaga
    21001 caccaggctc aatacaaaac gccacttgtc cattaatctc aaaacgaacc atataattta
    21061 aagtgaagtt tagattgttc ggactagaag taacaccaat attttcgccc caatcaattt
    21121 tccgaccacc taaaacagct gcattggttg cacggcgaac acgaaccgaa cgattggttg
    21181 gtactgttac taacagtgta ggtgtacctg tttttggaat gttctgctct tttttctttt
    21241 gattttcaat actagccgta gattctttgt ttggtttcgt tggttgattt tgactagctg
    21301 cggttgaaga gattgttttt tcaacgtttt cctcattttt tccttctttg tcagcagtag
    21361 ccgctggttg ttccactgtt tcttctattc gtttcttttc ttccgcttgt ttctcagaag
    21421 actctggctg cacagaaggc gttgcttcgg cagctttttc tccttctaga gtggacgttt
    21481 ctgaagcagg ggttgtaatt gaggcagttt cttcaactgg ttgtgaagta ccaggactat
    21541 tccctgggtg ttgtgtttcc ccaatcgtct gatcgagagt cgttttggaa ttgaccgttg
    21601 gcgcttctgt cacaggggcc tgttcaacag tggcttcacc agctgggacc ccggcttctg
    21661 ctggttggac ttctgtcact tcagaagcga cttgggcaat ggcttccatc gtaggttgta
    21721 tagccgtcgt ttcttctgca tgtgcttgag cagttcccac caagaaacag aattgagaaa
    21781 aagccaccaa tgcgagtgct ttcaaccaat tcattttcgt ttttgcatac acaagaattc
    21841 ctccttttcc tgagaatttt atataataaa acaaaacgcc gttaccaaaa gtgataacga
    21901 cgtgaactgt ctcttctatt tttatctggc gctcttatct gaacacattt catcaatgat
    21961 tttaccctcc cactataatt acagaacaaa aaaagaacct gcttcgtgga aatcaactgt
    22021 ccatttccca attggaatac cagctttttt ggcaacttca ccatcaccaa atctcatgcc
    22081 ataccaatta ctaccttcat ccttaatttc tttccaacca tatgacatag cttcatctaa
    22141 tgaatcaaaa atcatgacac tattgccaat tgttacagga tctggcttaa gatcttctgg
    22201 aagagaaata tcaatcatac cataatgatc cgattcctca gtattattgt cattactagc
    22261 tactggtttg gttgttctac tggcttattt tgatacaaca agtactctta ttcttctttt
    22321 acttctccgt tttcaatttc ccattcaaac cactccgcaa agtcttgctt aaaaccatct
    22381 actcgatacc tattttgtaa ataggcatct tctgacgtcg taggatctaa ataggtaggt
    22441 ttattctctg gacgagcata gacacctacc catgtttcgt aatctccttc aaaataacta
    22501 tctaccaaat agcctttttt tctaagaatc tcccgaactt gatttttaat tattgatact
    22561 tgttcaaaac catcactcat tctatcacct caaataactc tagattctca taaatattcc
    22621 cgacaatttc aatcctatca atgatctcta acaaatcgct acttatgctt ccccattcgt
    22681 ataagaattt cccttccgca aaataaattt tccataaact tcttcatgat cgtcccaacc
    22741 aatatcactc tcgaaaaatt ccacgttgtt cttatctttt agctcagtaa atgccataag
    22801 ttcaacatgt tttccaacct cgaaacaatc acaccaccca tcaaccgtta caagtgtgcc
    22861 ttcaatacct aagaaacttt gtcctcgtgt attaaacact aaaactttcc gcattttttg
    22921 ctcattaatt aatcacgcgc gaaatctagg aatcacaact tattcgcttc ttctttataa
    22981 ataaaaaaac tccaataatc ggaaattact atcttttcct cattcctttt ttaaatcttc
    23041 atacttcttc ttatctttgt cctaacctaa taacacaaat tttgaagaaa aactcagtaa
    23101 aacctgattc tttttttatc ttcacaacaa ccaacttttt cttcagatcc ttttactaga
    23161 acttgtatac aaaaattgtt gaatgctgat ataatttgcg gctacttgaa aaaacgatag
    23221 atgtaaaaag ataaatagaa tcatttttta ttctatatca gtctatgatt cattgcttat
    23281 caatcaccca acccattcga tgccgttttt tttagagaaa tttaaaaaag accgtacgaa
    23341 tctcgcatta tccgattact tttccagaaa agatccccaa ttctgtataa ataaccctat
    23401 cttcttgttg gtttcatttt ctcatataag aattcagtca atctacccac tatgaccatt
    23461 actacgtcca acacaactga catattaacc acgtaagtat tccctcacag atctccgaat
    23521 ctccatggta tggattccca tcctgacgct gcagaggaca acatatttca tgtggctctg
    23581 tacccttttg atttggaaac actacaaatc aatactcatc tataacttat caagcgaact
    23641 ttcgctaggc ttctagtgat tactaaattg ttggataaca tacatagccc attttatcta
    23701 gctgttattc ataacagtta gttcttgtat tacttcttcc gcttgtgttg ccattgagct
    23761 tgtcaaaaag ttacttagaa aaaagctgcc agtaaagcaa ctttcaacaa accctttttg
    23821 tttcttatca tatcaagaaa cctcctttta taaatttttt ctataataaa aaacgtcgat
    23881 aacttaataa gtatcgacgt aaactatttt tatatc
//
LOCUS       DACDHL010000039        23828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDHL010000039 DACDHL010000000
VERSION     DACDHL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.039"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(54..788)
                     /locus_tag="IV228_001550"
     CDS             complement(54..788)
                     /locus_tag="IV228_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8717448.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="IV228_001551"
     CDS             1115..1525
                     /locus_tag="IV228_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8717449.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="IV228_001552"
     CDS             1518..2219
                     /locus_tag="IV228_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8717450.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="IV228_001553"
     CDS             2219..3805
                     /locus_tag="IV228_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8717451.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="IV228_001554"
     CDS             3824..4045
                     /locus_tag="IV228_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8717452.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="IV228_001555"
     CDS             4042..4803
                     /locus_tag="IV228_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8717453.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="IV228_001556"
     CDS             5183..5692
                     /locus_tag="IV228_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8717454.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="IV228_001557"
     CDS             complement(5767..6306)
                     /locus_tag="IV228_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8717455.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IV228_001558"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IV228_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8717456.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7373..8170
                     /locus_tag="IV228_001559"
     CDS             7373..8170
                     /locus_tag="IV228_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8717457.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8198..8833
                     /locus_tag="IV228_001560"
     CDS             8198..8833
                     /locus_tag="IV228_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8717458.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="IV228_001561"
     CDS             8853..9626
                     /locus_tag="IV228_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8717459.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="IV228_001562"
     CDS             10034..10831
                     /locus_tag="IV228_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8717460.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="IV228_001563"
     CDS             10809..11222
                     /locus_tag="IV228_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717461.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="IV228_001564"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="IV228_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8717462.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="IV228_001565"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="IV228_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8717463.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="IV228_001566"
     CDS             15999..16559
                     /locus_tag="IV228_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8717464.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IV228_001567"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IV228_001567"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8717465.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="IV228_001568"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="IV228_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8717466.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="IV228_001569"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="IV228_001569"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8717467.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="IV228_001570"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="IV228_001570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8717468.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="IV228_001571"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="IV228_001571"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8717469.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="IV228_001572"
     CDS             21906..22484
                     /locus_tag="IV228_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8717470.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="IV228_001573"
     CDS             22536..23018
                     /locus_tag="IV228_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8717471.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="IV228_001574"
     CDS             23135..23509
                     /locus_tag="IV228_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717472.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6646 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtt
//
LOCUS       DACDHL010000040        22485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDHL010000040 DACDHL010000000
VERSION     DACDHL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.040"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..697)
                     /locus_tag="IV228_001575"
     CDS             complement(<1..697)
                     /locus_tag="IV228_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717473.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFV"
     gene            835..2349
                     /locus_tag="IV228_001576"
     CDS             835..2349
                     /locus_tag="IV228_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8717474.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2752..3735)
                     /locus_tag="IV228_001577"
     CDS             complement(2752..3735)
                     /locus_tag="IV228_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8717475.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3740..4387)
                     /locus_tag="IV228_001578"
     CDS             complement(3740..4387)
                     /locus_tag="IV228_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8717476.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4472..5104)
                     /locus_tag="IV228_001579"
     CDS             complement(4472..5104)
                     /locus_tag="IV228_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8717477.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5120..5671)
                     /gene="coaC"
                     /locus_tag="IV228_001580"
     CDS             complement(5120..5671)
                     /gene="coaC"
                     /locus_tag="IV228_001580"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8717478.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5668..6405)
                     /locus_tag="IV228_001581"
     CDS             complement(5668..6405)
                     /locus_tag="IV228_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8717479.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6402..7391)
                     /locus_tag="IV228_001582"
     CDS             complement(6402..7391)
                     /locus_tag="IV228_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8717480.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7649..8191
                     /locus_tag="IV228_001583"
     CDS             7649..8191
                     /locus_tag="IV228_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8717481.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8208..9293
                     /locus_tag="IV228_001584"
     CDS             8208..9293
                     /locus_tag="IV228_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717482.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9305..10111
                     /locus_tag="IV228_001585"
     CDS             9305..10111
                     /locus_tag="IV228_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717483.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10112..10939
                     /locus_tag="IV228_001586"
     CDS             10112..10939
                     /locus_tag="IV228_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717484.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            10936..12009
                     /locus_tag="IV228_001587"
     CDS             10936..12009
                     /locus_tag="IV228_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8717485.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            12437..15133
                     /locus_tag="IV228_001588"
     CDS             12437..15133
                     /locus_tag="IV228_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8717486.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            15210..15698
                     /locus_tag="IV228_001589"
     CDS             15210..15698
                     /locus_tag="IV228_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8717487.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            15710..16204
                     /locus_tag="IV228_001590"
     CDS             15710..16204
                     /locus_tag="IV228_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8717488.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            16225..17034
                     /locus_tag="IV228_001591"
     CDS             16225..17034
                     /locus_tag="IV228_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8717489.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            17031..17852
                     /locus_tag="IV228_001592"
     CDS             17031..17852
                     /locus_tag="IV228_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8717490.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            17903..20002
                     /locus_tag="IV228_001593"
     CDS             17903..20002
                     /locus_tag="IV228_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8717491.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            19999..21117
                     /locus_tag="IV228_001594"
     CDS             19999..21117
                     /locus_tag="IV228_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8717492.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            21156..22034
                     /locus_tag="IV228_001595"
     CDS             21156..22034
                     /locus_tag="IV228_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8717493.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            22225..22377
                     /locus_tag="IV228_001596"
     CDS             22225..22377
                     /locus_tag="IV228_001596"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717494.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6979 a         3893 c         4827 g         6786 t
ORIGIN      
        1 taacaaataa atccttcata gtatccatga ttcccacgtc taagtaacca ttttggatga
       61 ttgttttatc tgttttacca tctgcatttt gactttcaat gattgtccga gtgatatctg
      121 gaacatgata gacagtgttt ccgtctttac ttaactccac actattgatt tgctggatga
      181 tcgtatcttt ttgcatatcg aattgcagta atttatctgc tgaaaattcc acaaactccc
      241 acataacacc aactgtcatc gaaaaacaga aggaaacaac tgctagtaat aaaggagtca
      301 agttcatggt ttttgtattt ttattgagca aataaacaag ggaaaagccg acacccccag
      361 ctaaaaatcc gctgaatgca tgtagaaaag tatcccacat tgaaaaatag ccataaaaat
      421 cacttaactc tcctaaaact gttgaactaa aaataaaaga taacaaaatg atttctagca
      481 tatttggaat ctgtatcttt aatatctttt ccagaaaata agggatttca aataaaatta
      541 atgtcagtaa taaaagcatt gtatttcccc agtgttggtt ccagacctga tccactagca
      601 taataacgac gatgatacgt aataaaatat agattccgtt tgtgattcta cctgcagttt
      661 ttgtactgtt cgtaaaaaag gttttcaaat gattcataaa ataactcctt catgtatatc
      721 cttaattata ctaagaagcg tgtgtttacc aaagctatat gacttaaaga aaaacagagt
      781 tgatattgtg aaaaaaatca cttaatatag tataaggaga aaagggggat acaaatgaaa
      841 aaattagttg ctggtttatt aacagccgtt gcacttcttg ctatgagcgc atgcggcgca
      901 gatcagaaaa atacagcgca gacaagttct agtacgaagg aatctgccga agtatcatca
      961 ggcgcttctg aacaagtata tacagatcca aaagaaatga aagaatctta tgatgtcgtg
     1021 attattggag ctggtggtgc agggatggca gcagctatcg aagcaaaaga tgctgggttg
     1081 aatccagtaa ttttggaaaa gatgcctgtt gccggaggaa acacatctaa atcatcaagc
     1141 ggtatgaatg cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga ctcgaatgat
     1201 gccttctatg aagaaacgct aaaaggtggt ggaggaacga atgataaaga aatgcttcga
     1261 tactttgtcg atcactctgc tgatgcggtc gattggctag atcaaaacgg tatcaaactg
     1321 gacaacttaa cgatcactgg agggatgagt gaaaagagga cacatcgtcc gtctgatggc
     1381 tcggctattg gtggatactt agtggagggg ttattgaata acgtccatga gcgggaaatc
     1441 cctatttttg tcaacgcaga tgtgacagac attaagaaaa atggggaaac ggtcaatgaa
     1501 gtgactgtac atgttcaagg agaagaacca aaagaggtca ccggaaaagc aatcgtagtt
     1561 accacaggcg gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc cgatttagct
     1621 gattatatta cgacaaatca agaaggatca actggagatg gtatcaccat gattgaaaaa
     1681 ttaggtggac aaaccgttga tatggataaa atacaaattc atccaactgt gcagcaggat
     1741 gagggggtct tgattggtga ggttgttcgc ggagaaggag caatcttggt cgatcaagag
     1801 gggaaaagat ttgtcaatga aatggataca agggataaag tatctgcagc aattacagca
     1861 ttgcctgaaa aatcagctta tttgattttt gaccaaggcg tgcgtgatcg tgccacagct
     1921 attgaatttt atgaccaaaa aggatatgtc acaactggaa agacaatgga agaattagca
     1981 gaaaaaatcg atttaccaaa agaaacacta gcaaaaacag ttgatggatg gaatacggat
     2041 gttgcggaga aaaaagatgc tcaattcaac cgtgccacag ccatggaaca tgacttatcg
     2101 tctccgaatt attatgcgat caaaatcgca ccaggaatcc attacacgat gggcggtgtg
     2161 aaaatcaata cgaacactga agtattagat aaagaaaacc agccaattag ggggttatat
     2221 gctgctggtg aagtcacagg tggcttacat ggaaacaacc ggatcggtgg aaattcagtt
     2281 gcggagatca ttatttttgg gcgacaagcg ggcatacaag cagctcgatt tgcttcggaa
     2341 aatgaatgaa atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt gagctaatac
     2401 cgagggtcag ctgattgaac actgtttagt gaagaggttg tgaaaaagct gccctgcatc
     2461 aagtaataag aacagccaag taaaaacagc ttcttatgtt tttaacttgg ttggacttat
     2521 taccgcagat gcaagctttt gaacaccgtt tattcggaaa aagagcgtgg gacaaaagta
     2581 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agaaattcct
     2641 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
     2701 ttcttggagt taaacgcttc tgtcccagcc tcttttttga tcagcatgat tttattcagt
     2761 tatcttcaaa atgatttttc cgatgttttt gtttgcttcc atatattcat gcgcttgttt
     2821 gactgcgctg aattggaatg tacggtcaat gattggccga atcttgccat tttctagaaa
     2881 aggcccagct ttttgcataa aagtttgtgc gagctctgct ttatactgat cacttctagg
     2941 tgtcaataat gtaccaagca gttggacacg tttcccaatc aatacaccaa ggtctatctc
     3001 aggcacgatg gtcccaccta gcataccaat caagacccaa cgtccatcaa cggctataga
     3061 agctaaattc ttttcccagt aagaagcgcc gatgaaatcc aaaataacat cgactccccg
     3121 accttcagtg atttttcgta cctcttcttg aaagtcttgt tttttatagt tgatcaattc
     3181 atctgcacca agttctttac aaaaggcgag tttttcttct gatcctgcag taacgatgat
     3241 tttcgcgtga gaaagctgtc tagctaattg gatcgctgct gttcccacac cacttgctcc
     3301 tgcatggatc aagacagttt cgccttcttg taattttcca agccagtaaa gtgtttgata
     3361 agcagtcaaa aaaacttctg agattccagc agcagaaaca tagtctagcg tatcagggag
     3421 aaggattgca cgatcggcag gcatcacagc atattcggca taaccaccta gattgactaa
     3481 tccgtatact tttgtcccag gagtaaaagt ggagatatca cttttattct caactacctc
     3541 tccagctact tcgactccca aaatagggta gggaggtttc aaagagactg attctctttt
     3601 cataatatct gttcgattga cagctgctgt atggactttg atcagtaatt cgccttgttt
     3661 tggtttagga acaggcactt caatttgttc taatacatct acagaaccag gatgcttgat
     3721 tgaaatggca cgcatggatc tattcctcct ttgcccattc ttggaatgct gtattcaact
     3781 ctttgctacc ttcagaaagt actagagtcg tacgagaaat atccgtttct tccactagag
     3841 cggcaagtac agcagcaact gtagagcgag tgatcgtttt tgatgccttc tcattaggct
     3901 tagttaattg aatggaagta acttcttcat cgttagtcaa tgtgactggt tgaagaatga
     3961 caaaatctaa gttagttcgt ttcagccatt catctgcata atgtttagta atataataat
     4021 ctgtcatgga ctcaggccat tttgctggtt catctgcata aacagcactg accatcagat
     4081 agcgagagac atgcgcattt tcagctgcga tcactgtttt gattgcgcca tccaagtcta
     4141 cttgcagaag attctttcct tgagagccgg cagcaaaaat caatacatcg atgcctttga
     4201 atgcttcggt cattttttcc acggaccaag ttaaatcaaa agagacatac gaaacattct
     4261 ctttttttac taatgtttgg gaagcaacat ctctcacccc tgcaaaaatt tcatgggaac
     4321 ttggagctaa atcttttatc aggtgtcggc caatttgtcc gtttgcacct acaacaaaaa
     4381 ttttcatttg gatcatcctt tcgacgtcaa atctttccat tcaatagtaa caccaaaaga
     4441 gaaaaaaatc agagatcggc tacgatgact atcagttagt aagagaaggg tgcttgaagt
     4501 atcttaacaa gacattgcat actgctgcag tcgcaagacc tgcgacgatt ccttcaggta
     4561 taccacttgt aaaaataacc gctagaatag ctgaataaac agcatttaaa tttgttccga
     4621 tgactttggc ataatcttgc tggaataaaa agtagatcat attcatcact aaaatcgtat
     4681 tggtcaatga accggaaagt ccagctaaga ataaagaaac ggaatctgct ttttgtttca
     4741 ttagtttgcg cagaccttta taaacgaaat aaggaaccac cccaatcaag atgcgaggaa
     4801 taaacgcgat cagcaaggct ttccagcttc catgttctgt tccgatgact gggataaacg
     4861 gagaaaagac aaaagacaaa ggtgtttgga taactgtgct acggatcatg ctgatcagtc
     4921 caaatgtacc gccaagaaaa gcacctagtc ttggtcctaa aacgatcgaa gcaatgatta
     4981 caggaatatg catggttgtt gcattgattg gaccgatagg gataaaacct aaaggcgtaa
     5041 cagctaataa aatcaaaata gctaaaaaca tcgcagttaa tgtaaagttt tttgtatttt
     5101 tcataagtac tccttttttc taagcatttc tttcattaag agtcagcatg acctgttcga
     5161 tgatcgtctg aatatcagct aaggcacctt ttccaaaatc tccacaagct aaaagagatt
     5221 ctcttggttc aatttcctga tagccgacag atctcaatgt ttccagatta cgctgagtga
     5281 tcgggttttg atacatattc gtgttcatgg caggggcaat caattttggt gttcccactg
     5341 gaagcgccat tgcgactgtt gaaagaagat cgtctgcaat gccgtttgct agcttgccga
     5401 taatgtttgc tgtagcaggc gcgatgagaa acaagtcagc ttgttttgct agttcgatat
     5461 gattgattac ctgcggattt gattcttgca tgacgtctgt atggactgcg cgcttcgaca
     5521 gagattgcaa ggttaatggc gtgataaaat gtgtgctgct tttagacata atcacatcta
     5581 cctgggcacc taatttgacg aattgacttg taagatcagc gcttttgtaa gcagaaatac
     5641 ttccagtaac gcctagtaag atctttttca ttttgattcc tccagtctaa ttttctcagt
     5701 aatcaatgca gcaatctcag atttactttg agcttcttcc actgttccat ctttagataa
     5761 gagatagccg tggtgtgtct cgccatggat ttccgtcaaa tcatttgcga aaataaaatc
     5821 agttttgtta tttatcagac tattttttgc gactgccaga agagattcct ttggtacatc
     5881 tactaatagt ttgaaaccaa tgatttttgc ttttggctgc cattcacgta gataagaaat
     5941 gatttttggt gttttcttca aaacgagcac aagatgctcg gtgttagaag agatctttga
     6001 aacagtggta tctgtctgtt cattttccgc aaaccaattg tccagttctt cttgcgttga
     6061 agaagtgggt aggttttttg ccagctgttc ttctgaaaaa ctaaaagcag gagtaaaatc
     6121 actgactgcc atactgtgga taatcgcatc gtagtgttct tttttcgtta gttcttctaa
     6181 ttgtaaaaat aagtccagtg cagattcgat cgtataaatc gttatattgg agtgtctttt
     6241 cggtttcagt gcttgtttcg tagtcacgta atcgattgtg gtatccggat tggcagctaa
     6301 ataatcagct aatgcttgcc ctaatcgtcc tgtcgaatga tttgtaatag cacgtacttg
     6361 atcgattttt tcagtcgttc ctccagctgt gattagaatc ttcatttatg gtcacctaca
     6421 tttactagtg ttcttccttg gtgtgttcca gcaagtagtt tagatgctgt ttcaggcaat
     6481 tgatccagag taatctcttg gacgaccagt tcatctgcaa tctgcaaatc cgccatctgt
     6541 tgccataaag aaagacgttg atctattggt acattgacag aatcaattcc aatcaactgg
     6601 atgtttcgca gaataaaagg caagacagtc gcgtttagtt tgattccccc agcattgcca
     6661 cataaagcta ctgcaccgtt ataagagatc aatggaagaa gactgcttag ctgctcgcca
     6721 cctactgtgt cgatgacata gtcgatttgc tgttttccta aagcttttgt tgtctctggc
     6781 agaaattcat caggtcgtac gacttgcaaa gcacctagac tttttagcca tgtgacagac
     6841 tcttcttttc tggaaaaggt aatgattgac tggaatccta gtttatgtaa cagggctatc
     6901 gctgtactgc ctacaccgcc agaagcacca gtaacgacaa cttttttatc ggaggtcata
     6961 ccttgacgga ttaaagcgtt cacacacaac atagcagtaa accctgctgt accaaggatc
     7021 atcgcttgac gcagcgtcat attttttggt agaggaacta accagtctcc tggtactttt
     7081 tgatattggc tataaccgcc tggatgggtg acgccaagcc caaaaccagt agcaagtaca
     7141 agatcttctt ttgaaaaacg tggattgtta ctttggacga cagttccgga taaatcaatc
     7201 cctggagtca tcggatatgt tcgtatcacc tgaccagact cgctcattgc caatgcatct
     7261 ttataattga catcagaata ggcaattttt accaagactt cattttcagc caactcagta
     7321 aatggcagat cactgattgc tgtatggaaa acaggttcct ttattacctg aaaagatcga
     7381 aaagcgttca ttcgtgagac actcctttta aaattttctt ttatcatacc ataaagccta
     7441 ctcttttatt cttgttcttc cgtaaattta gacaaaaatt aggtgaaaaa taaagaatca
     7501 aacaactaga atttattcca aaagataaag aaatattcag caaaaaaaat tttacaaagg
     7561 gttgcctttt tcaataaatt tcgtatacta atacgagtgg tagtaaacag gaagtttact
     7621 attagaaaac ttcgattcgg aggaaattat ggaaattggt gagaagttaa gaaatttgcg
     7681 catccaaaag aatttgacac aagaagaact aggcgaacgc acagatttgt caaaagggta
     7741 tatttcccag ttggaacgag atttaagttc accatcaatg gaaacattct tttctattct
     7801 tgaagtctta ggtgtgactc cagaagaatt ttttcatcaa gaagcagcga atctgcaagt
     7861 ggtttattca aaagaagatc atacggttta tttcgatgaa gaaaacgggt atgaattgga
     7921 atggctcgtg gctgattcga atgaaaaaga aatggagcct gtcttgctga cttttgaagg
     7981 tgcaggagaa tataagacgt ttgaaccttc attatctgaa acgtttattt ttgtattaga
     8041 aggggaatta gagttaagct taggagagac ggtctatgtg gcaaaagcag gacagtcgat
     8101 ctattatcaa gcgacaaaac aacatcaatt aaggaatcat ctcaaaaaac gaacaaaagt
     8161 attgattgtt gcaacgcaat catatttgta atataggggg taaaaacgtg gggaaaccaa
     8221 tcatttcatt tgatcaagta gtgaaacgct atgatgatga aacagtatta aaaaaagtaa
     8281 gttttgagat cgaacaagga aaattttata cactgcttgg tccttccggc tgtggtaaaa
     8341 caacgatcct acgtattata gctggattca ccgaagctac agaaggggac atctattttg
     8401 aagggaaacg gttgaatgat ttgccagcta ataagcgaca agtaaataca gtcttccaag
     8461 attatgcact ttttcctcat atgaatgttt ttgataacgt tgcttttgga ttgaagatca
     8521 agaaaatgtc caaatcagat atcgaaaaga aggtaaaaga tgctttgcgt atggtccagc
     8581 ttcctggata cgaaaaacgg gagattagtg aaatgtctgg tggtcaaaga cagcgtgtag
     8641 cgattgcacg agcaattgta aatgaaccga aagtattgct attagatgaa ccgttatcgg
     8701 cgcttgattt gaaactacgt acagatatgc agtatgaact gcgggatctg caacaacgat
     8761 taggaatcac gtttattttt gttactcatg accaagaaga agcgttggca atgagtgatg
     8821 agatttttgt catgaataaa gggaaaatcg tccaaagtgg tacacccgta gatatttatg
     8881 atgagccaat caatcatttc gttgctgatt ttgttgggga gagcaatatc gtggacggtg
     8941 taatgatcga agataatcta gttgagtttg ttggtaagca atttgaatgt gtcgatggcg
     9001 ggatgcgtct aaatgaacca gttgaagttg ttttacgccc agaggatttg acgatcacga
     9061 caccagataa aggaaaacta gtcgtaacgg tcgatactca gctgtttcgt ggtgtccatt
     9121 atgaaatcat ctgttatgat gaacagcaaa atgaatggat ggtccattca acgaaaaaag
     9181 ctaaagaagg aagcaaagta ggattggcct ttgagccaga agatatccat gtcatgcgtt
     9241 tcaacgagtc tgaagaagaa ttcgatgcac gtcttgacag ctacgaagaa taggggggac
     9301 aagaatgaaa acaatgcgca ggatctattc cgtcccttat attttgtggc tcggtttatt
     9361 cgtgatcgcg cctgtattaa tgatcattta tcaatctttt tttgatatga atgggcaatt
     9421 cacgctgaat aattatctat cttattttac gtcggggaca tcgctttcta tgacgatcaa
     9481 ttctgtatgg tatgcatttt tgatcacgtt tttcacgctg atcatcagtt acccgactgc
     9541 ttatttttta acaaaactga agcacaagca attatggctg atgctgatta ttttgcctac
     9601 atgggtgaat ctgctgctta aagcttacgc atttattggg attttcagtg tgcacggcag
     9661 cgtgaatcat ttctttgatt ttatcgggat cgggccgaaa cagatattat tcacagactt
     9721 cagttttctg ttcgtggcaa cgtatatcga gattccattt atgattatgc caattttcaa
     9781 tgcattggaa gagatgaatc cttctttgat cagtgctagt agagatttag gcgcgaatag
     9841 ctttgagacc ttccgacgag tgatttttcc gttatcatta aatggtgtga aaagtggtgt
     9901 ccaagcagta tttattcctt ctctttcttt attcatgctg actcgtctga ttggcggaaa
     9961 tcgggtgatc actttaggaa cggctatcga acagcatttc ttggttacac aaaactgggg
    10021 gatgggttct acaatcggtg tgattttaat cattgctatg ttcatcgtga tgctcctgac
    10081 aggcgagaag aaaaaaggag gtcgctcata aatgaaaaaa tttaaatggt cgtatcttta
    10141 tttagggatc gtctttttat tcttatatgc accgatcttc tatctgattt tttattcctt
    10201 caatgaagga ggaacgatga acagctttac aggatttact tgggaaaatt atgaagctgt
    10261 ttttgaagat acacgtttga tcattatcgt attgaatacg ttcatgctgg ctttcttatc
    10321 tgcattgatc gccacgatca tcgggacatt tggggctatg ggaatctatt acacgaaaaa
    10381 acgacgtgca cggacagcgt tgttgagcat gaataacatc ttattagtct ctcctgatgt
    10441 tattatcggt gctagtttct tgattttctt tacactacta ggattcggat taggttttca
    10501 gagtgtattg ctttcccata ttgctttcag cattccaatc gtcgtcttga tggtcttgcc
    10561 aaaattgcag gaaatgaacg attcaatggt cgatgcggct agagaccttg gtgctaataa
    10621 tttccaagta gtcaaaaata tcattttgcc ttatctttct ccaggaatca tagctggata
    10681 ttttatggca tttacctatt ctcttgatga ttttgcggtt acatttttcg tcacaggaaa
    10741 tggctttacg acgctctcag tagagatcta ttctcgtgca agacaaggaa tcagtttaga
    10801 aatcaatgcg ctaagtgcgt tagttttctt gttctccatg atattggtca ttggatacta
    10861 ctttattagt aaagaaaacc gtccaaaacg gctgaagaaa aacagaagag tacgcgaggg
    10921 ggtggcacgg ctgcgatgaa aaaattacaa tctctgtttt taggtattct catcattatt
    10981 gcggtgctgc tgcttggtgt tcgtcaactt gaaaaaacaa gtggcatgag tggagcaaag
    11041 gtacttacca tctataactg gggagattat atcgatccta gtttgatcac gaaatttgaa
    11101 aaagaatatg gatacaaagt gaattatgaa acatttgatt ctaatgaagc gatgttcaca
    11161 aaaatccagc aaggtgggac aaactacgac atcgctatac caagtgaata tatgatccaa
    11221 aaaatgatca aagaaaaact agtacttcca ttagatcatt cgaagatcaa aggattaaat
    11281 aatattgatc cacgattttt agacttggat tttgatccag ataatcagta ttcgattcca
    11341 tatttttggg gaactttagg gatcgtatat aacgataaat tctttgatgc aaatcaaatt
    11401 aagcattggg atgatttgtg gaagccggaa ttaaaagaca gtttgatgct gattgacggt
    11461 gcacgagaag ttatggggct ttctttgaat agtttaggct attctttgaa tagtaagaat
    11521 atggcacagc tgacagaagc agcaaataaa ttgaatcatt taacaccaaa tgtcaaagca
    11581 atcgtggcag atgagatcaa aatgtatatg atcaatgaag aagcctccgt ggcagtgaca
    11641 ttttctggcg aagctgccga tatgatgtgg gaaaatgaac atttacatta tgtgatccca
    11701 ccagaaggat ccaatctttg gtttgataat atcgttatgc caaaaaccgt gaaaaataaa
    11761 gaaggtgcct atgatttcat caactttatg ctagaaccgg aaaatgcggc acaaaatgca
    11821 gagtatattg gttattctac accaaatgaa gaagcaaaag cgttattgcc aaaagacatt
    11881 tcgtcggacg aacaattcta tccatctgat gacacgatct cccatcttga agtctacaaa
    11941 gatctaggat cgaaatacct aggcatctat aatgacctat tcttagaatt caagatgtat
    12001 aggagataat ggcaaaactc agtaagaatg gggacttatc aacgttctaa agagctaaaa
    12061 atcgtccttt ttctatctga gttttccaat cttctaccaa aagcggatga aacgccgctt
    12121 tttttgtgtt attttatttt ttctaccacg tttttctacc agagaaaagt ggacaaaacc
    12181 attgtaaaat agggcccgag acgggctagt aaatctaaga ttgaaaaagt tacagaagga
    12241 taaagtaatt ggaaactatg caaatatact taacccttct gtaatctatt aataaaacta
    12301 atatatgaat ccagttcaat atttgtagct atgaaaaaac ataaaaatca tattagataa
    12361 aaactaaatt ggtagataaa ttattttgtc agatatctga ggcaaatatt cgactatttg
    12421 tgtttcaaag taaaaattgg aatacaaata tagaatagac atattgcagc cgatagttta
    12481 ttatgctttt agacacatta aactggagga aaaaatgaaa aagagcgcta aagatcgagt
    12541 ttttgatgag ctgatacgac taacagggaa tatgtcttct gtcactgccg agaaaatttc
    12601 gaagaatcta ggtatctctc gtcagaatgc cagccattat ttgacgagat tagtggaaga
    12661 taaaaaggtc gagaaattac cgggaaagcc tgttttgtgg aagcctttag atgaatatgc
    12721 ggttgtagat aatacggagc aaataaacga agcctttcat tctgtagttg gtcatgacgg
    12781 aagtctgaga gaggtcattc aaaaatgtat agccgctgtc aaatatccgc ctaacggact
    12841 cagtgtatta ataaatggag ccacgggtgt tggaaaaagt tttttagcca caaaaatttt
    12901 tgaatacgct atccatgagc aaattattga aaaagatgca ccatttgcta tcttaaactg
    12961 tgccgattat gcggacaatc ccgaactgct ttcggcaaca ctgtttggat acaagaaggg
    13021 ggcctttacg ggggctgaaa aggataccga tggtttattg gcaacggcca acaacggcta
    13081 tctttttctc gatgaggtcc atcgcctatc gaaagaaaat caagaaaagc tctttctatt
    13141 tattgatact ggaaactatc gtccagtcgg tgaaaatgta aattggcatt ctgctaaggt
    13201 tcgttttatt ttcgccacga cggaaaaagg tgaaaattat ttattagaca cctttgatcg
    13261 gcgtattcaa attagtgtta tgttgccaac ttttgaagat cgccccattc gagaacgatt
    13321 agagctgatt caattatttt ttcaaaatga agcggatgtt ttacaaaaag atattattgt
    13381 ttcaagagaa gccttgtctt taatgttatc tcatcccttt agtgggaata ttggtaagct
    13441 gaagaatatc attaaaatca gttgtgcgga tgtgtatagt cgaacacaag aagaaccatt
    13501 aacgattggg aaaaatgaga tcttgattca gttaaatatg ctggaatccg aagtagaaag
    13561 cttgccgatt gctagtttat tgataaagaa aaatcaaaag ataaaaacgg atcgaatcaa
    13621 accgtttgat tatgattcga ttttggaaaa aattactcag ctgataaatg aaggtaattt
    13681 caatgaaaat ttttcttcga taaagaatga agtacagcat ttggtgcatc acattgataa
    13741 ggattctttt ttatcaatga gtagaaaatt gatgattcag atgtttagaa aggtctgggt
    13801 atccgtttta ggaaaaacgt acggattgac gacgagtgga cctgttgcag aaattattag
    13861 cagatcctat gtcatttccc caagctataa gacagactta accgggataa atcgtacatt
    13921 agctgcaaat ctacctcgga cgacttactt atgtaatcag ttcttggagc atttaccacc
    13981 gctctcagaa aaggacgaga actttctaag actgcttatt gtcgtgactt tatctgatta
    14041 tgtagatgaa tccatcgagc tgaagggcct gttgcttgct cacggagata gtacggctag
    14101 cagcattcaa gcagtagtga atcaattatg cggcaattat gtttttgaag cgttggatat
    14161 gccagtagag agtaatgtgt cagagattgt cgaatcgaca aggaatttcg tgaaacggca
    14221 agtgaataca gatagtctag tgctgatcgt agatatgggc tctttaaatc aaatttacac
    14281 acagatcaag aacgatttaa aaggggagtt acttcttctg aataatttga cgacttccat
    14341 tgcacttgat attggcttga agatgacgat gaatgcgcca tttaaacaga ttgcggagga
    14401 tgcagcagac aagtattcaa tcaatgttca atactttgaa ggcttttctc aaagtagcaa
    14461 cattatcatt tcctgtatgt cgggtttggg gatttcggac aaattgaaag atgtttttcg
    14521 tcatcattta tcggatgaat ccatcgaagt tttcacgaag gactacagag aactacgtga
    14581 attaatcgac caaaatgatg aaacttattt tgacaagaca aaattggtca tcacgacttc
    14641 ggatcttcca cgttcctttt caattcctaa tattaatgtt tatgacatct tagacggtga
    14701 tggagcagcc aatttggagc atattttgag tcctgaaatg aagccggcag aatttaaccg
    14761 cttgattcag gagctagtaa aattcttttc gttggaagga attgcggacc gtttgaactt
    14821 tctgaaccca acgatcgtga tgagtgaagt ggaaacagtc atttctaaat atgaaaatta
    14881 ctatcatttc actttaaatg gccgtgtaaa actaaatctc tatatgcata cggcattgat
    14941 gatggaacgt ttattcttgg ctcggacaga aaaatctgaa gaaccgaatt atgatttgaa
    15001 accagatgaa gatgaatttt attatgtttc tagaagtatt tttcaatcaa tggagatgaa
    15061 atataatttt aaagtcaatg cttatgagct ttcgctgctc tatgaattat taggaccgta
    15121 tattcaaaaa taaagtgtct aaaaaagtgt ctaatctgtt ttttttaggt tagacacttt
    15181 ttttaatgtg aattaacgtg attaaaccaa tgtttttaaa agttggcaca ggaattgctt
    15241 atataaaagt gaacagaaag aaaggaatta accatatgaa gaaaattcta atagcgactc
    15301 acggtcactt agcttcaggc tttcttagca gtattcaatt actgacgggg aaggttcaag
    15361 agattacggt gattaatgct tatgtggatg agtgtgattt tgaacaggag ttgaatgtat
    15421 ttgtaaacca ggtgaacgag caggatcaga tttttgtttt taccgattta tttggcggaa
    15481 gtgtcaatca gaagattacc aaaacctttt tggagaaaga gattgctgta actattttgt
    15541 ctggctttaa tctgccaatt ctgctcgaaa tagtattggc aaccaaggaa ttaacaaagg
    15601 aggatattca ggaactggta aataaatgcc aacaggaatt aaagatgacc attcttgatg
    15661 aggtacatgt agtaaatgaa gaggaagatt ttttttagga ggaagaaaaa tgattaaagc
    15721 agtgcgaatt gatgaacgat taattcatgg acaagtagcg atgacttgga caaagagtct
    15781 aaatttaacc gggttagtag tagcaagcga tgaggcggct accaacgata ttcaaaagat
    15841 gacattgaag atggctgcac catcgaatgt caaagtcatt attaaaacag ttgatgacgc
    15901 gattcatttg ttaaaagatc cgcgggcgaa ggacatgcgc ttgatggttt tagtccctaa
    15961 tgtcaaagat gcggtaaaag ttgcgaaagc tttcccaacg gaaatcgaat taatgaacgt
    16021 ggggaatgct gggaaaatga caccgccatc ggaagataag aaaaccttat caaaggaagt
    16081 gatgttaacg acgtcagaaa ttcaagactt aaaagaatta gttgaacttt atccagatac
    16141 cgtttttcag cctacaccgg cgatggaaaa acgttcagct atctctattt tgagtgattt
    16201 ttagaaaaag gaaggagaaa aattatgtat gatgcatttg tagtcgcttt ggccgttttt
    16261 attggagttg ctggacatga aatgttcggg atgtccatgc tgagtcgtcc gattgttgtt
    16321 gctccgttag caggagcgtt gatgggggat ttgcagacgg ggttgattgt cggggcgtca
    16381 ctagaagcca tctttatggg tgtcgtcaat atcggaatct ctagtacggc ggagccttct
    16441 ttggctgctg ggctggctac agcgttcgca atcaaaatgg gaggaaacgt ggatgcgatt
    16501 attcccattg tctttccact agcagtatta ggattacagt taatgaatat gattttttct
    16561 tttgtatgtg gaccgatggc tgctaaattt ttcaaatttg caaaatctgg tgatcaaaaa
    16621 ggaatcatca atctgcactt ctttatgtgg tttgtccact atgcgcttta tgctcttatt
    16681 ccattctttg ccgttttatt tggagcagat gttgtcaaaa gcgtcttgga taatattcca
    16741 gaggtcatca tgaacggatt aacagtcgct ggaaatctgc tgccggcagt tgggatggcg
    16801 atgctgttac ggatgctttg gagtaaaaat attgctgtgt ggtttttctt tggggccgtt
    16861 gttatggcgt atctcgagtt accattgatt gcaattgccg cagttggaac gattattgca
    16921 attgttatag cccaaagaga tctacaacta aaaaatatat ctgttcatgc agttcaaagc
    16981 gatgctgctg taaatgcaac gcttgaccaa gaagaggagg atttctttca atgaaattag
    17041 ctgatggaat ttctaaagat gagaaaaaat tggttcggaa gatgttttgg cgctcgatga
    17101 ctatgtatgc ttcggtgaat ccggtgacga tgggcggcgg tggtttcgcc tattctatga
    17161 tgcccttcat taatcatttc tacaaaagtg aagaagatcg agctgcggcg ctggaacgtc
    17221 atacacagta tttttcgacg accattcctt gcgcgtcctt tgtcatgggg attgcggcat
    17281 cgatggagaa ggaaaattct gaaaaagctg gttttgacgt ggaaagtatc aacgcgatca
    17341 aaacgagttt gatgggacca ttggctggaa ttggcgattc attgttctgg ggagtttggc
    17401 gtgtcgttac cgcgggacta gcgatcaact tagccaatgc cggaaatatt ttggcaccaa
    17461 ttatcttttt agttatgttc aatataccta attacttgat gcgatactat ggcggcgttt
    17521 taggctattc attaggctct aaatatattg aaaagctgta tgcgaacggg ttaattgaaa
    17581 ttctgacaaa agctgctagt atcttgggtc tgatcatggt aggtgcaatg acgatcagta
    17641 tggtcaaatt caataccgtt ttgagctttt caatgaaggg cgaagaacta atgaatgtcc
    17701 aagaaatcct cgatagtatt tttaaaggaa ttgtaccgtt attggtaaca ttgagctgtt
    17761 tcaaattact agataaaaaa gtcggcgtca tcaaaattat ttttggaatg gtcgtcttgg
    17821 gaattttatt gtcattactt ggaatcgcat agtaggaagc tgcagctact aggctgcagt
    17881 ttttttagca aggagggaaa agatgactat taatgaagta atttattcac ccaaacaccc
    17941 attttttaat gctgagttaa acgacggaaa tgtgactact tcaaacgaat acagctacta
    18001 tcggaatatt cctgatgtaa agggattgac cttcgatgta agtaatcaaa agacattagc
    18061 aaaccttgat gtttgcggga cgattcaaac gttgaccttt tatcgagaaa atttactaac
    18121 tgaggaaaaa ccaggtgttt gggtcaataa acagctctct caaaccaatt cgttatcctt
    18181 aaaggttgaa gtaaacggga ttgtctacca tttgtcagaa tcggatcatc gtattgaagt
    18241 cgatgtgatt caagactgtt tgcctagata tattcatcat tattcgacgt ttaaggtcat
    18301 tgtagtatcc tttgctccca tactggctaa taagcgttta agtatgcttg ttcaacaggt
    18361 ttacattgtg aatgaaacac acgaaagcct tcaagtttca gtgaaagatg tgccttcgta
    18421 tcaagagaag tattcggatc agcaaaatgt gttgatttct caaaaaggaa ataaggacac
    18481 gatcgcacaa caggaagtgg caagcttcgc cattgctttt gtggacccaa atgcttttga
    18541 agaagtaatg acttttcagg aatctgatat cgaaaaatgg ctgaaggaaa cattgaacta
    18601 ttttgctcag ctatttgggc agctgtcaat gccaaacgat agagttgtcc atctctatcg
    18661 tcgtgcactt tatcaaagct tttctagttt cggtatgaat cgacagggac aggtcgtcgg
    18721 cagcaattgg ggcagctatc ctgctactaa tcgaatttgg aataaggata tgtattacgc
    18781 atcattgcca ttcaccatgt ttgaaccgga gttatgtcaa aaaacggttc tatggtttga
    18841 tcaatatggc gttaagtttc ctggttcaaa atttacagga ggtatcaatc attctctgag
    18901 taattcgtta tccagtgcgt tactggctag tctttatttt gaacatactg gggatacaaa
    18961 attctttgag cggtgtccgc aggttctact gaatgcttca agaattattg aggacatatt
    19021 agctcaacgt cggtcagggg aaccaatgct ttttccatct gtttggttat cagacgcctt
    19081 tgcacttgga aagtatcata ccggcagcaa tctttgtctt tggaaggcct gtattggact
    19141 tgcagaaatt tttgaagccc tagaacaact gagtcttagc aagcgataca aaaacattgc
    19201 ctgtaaacta aaggaatcaa tttgtaaaca aatgactatc gatggtcctt ttggtgagca
    19261 atttttggag ggaatcggtg atgaggagaa aacaacctat tcagtgaagc actatcaaaa
    19321 accgattctt gagcaagggt taatcttttt aagtgatgtg attgtggatg gaaaaattaa
    19381 tttattgatg catgatggag aagaatcaga cactacgttg atgccttttt atcaattttt
    19441 agataataaa gatcatttat atcagaacac catggccttt tctgctagtt ctttcaatcc
    19501 aacctatagc gaggaaatca aaggcatcac ttggggatta gaatccggcg ccacttttcc
    19561 tggctttatc actgttttaa tgagcgagct gcacaaccct caagctttcg taacccgatt
    19621 agaagaacta atttgtttgg cagatttaga cggttcgtgg tggtggtggc cgtatcgctt
    19681 agaggctcaa caaggagaag tcgtgcgaga ctttggctgc ggcaaatgcg gctgggcatc
    19741 gggtcttttt gtcagcctaa tgatcagtca atatttcgga ttgaaattca aaaaaggagt
    19801 attgcaaatt gatcctgttg ataatttaac gttttcttgg aagaatttga agttcggtca
    19861 agccttcatt tcgattgaat gtacgcaatc cgagttgagt atttcaaatt tgtcagaaaa
    19921 gccattgaaa ataagtgata cgaaaaaaat tcttcacgta gaaaagggaa aaacgattca
    19981 cattcaaagg agaaaaagat gagtatacta aaaaatatta cccaatttaa aaaaatccag
    20041 agtcgagcaa tcactcccga aaatccaaca ggcgagaaag gtaaagctgc gatgttgcca
    20101 agcgagctag gaccaagccg caaaggtcgt ggcgcaattc cgctgacttc tggtgaagaa
    20161 actgtgattg cggatattac tggaacggga ataattcgtc atatgtggat gacgattcgt
    20221 gatcgaacag aatttggcag ttttgtttta cgtgatttaa tcttaagaat ttattgggat
    20281 gggtcggata gccctgctgt tgaaagtcct ttaggggatt tcttttgtaa tgggtttgga
    20341 gagcgttgcg aggtgaactc gttgccgata gtggttaatc ctacaggggg ctttaattcc
    20401 tatttcgaaa tgcctttcca aacatccgct aaaattacga tcgagaattt acatccgaag
    20461 gatatgtcct cattttttta tacgataaat tacgctttgg tggacgaatt ggaagaaaat
    20521 agcctatatt ttcacgcaaa ttggcatcgg gaacgcatta ccaaattaca ggaggattat
    20581 aagttaattg atcgattaaa gggtcaaggc tattacgtgg gtacttacct tgcgcttacc
    20641 tgcctagaac gttattggtg gggcgaaggc gagtttaaat tctacattga tggggatagt
    20701 gagtatccga ccgtaacctc aacaggttca gaagattatt ttggcggggc gtgggccttt
    20761 catgagtacg atgagttcgg acgtccacat gcgaaaaact attcaacagc ctttttaggg
    20821 tatccttacc aatcaaatag agatgccaca agagatcgtt ttgaaacggg aaaattgaat
    20881 gccgttcatg cctttggtga cgacgcaatg ccacgacatg gtctttatcg ttggcatctt
    20941 caagacccca tctgttttac cgaagattta tcggtcactt tacaacaaat cggcaacgat
    21001 gatgttcgct tatttgagcg gcaagacgat gttgctagtg tcgcttactg gtattcaaca
    21061 gaagcttcag gaaatactaa acgtcatttc actgatcgag aacgcttgcc gcgttaatga
    21121 agtgtttttc acatattcga agggaggaac ctatgatgat atatggggca attgaagctg
    21181 gagggactaa atttgtctgt gcagtcggta ctgaggaatt aacgattttg gaaagagtca
    21241 gttttcccac aacaactcca gttgaaacaa tgaagcaggt cattgatttt tttaaacctt
    21301 ttaaggaaca attggcaggc atcggggtcg gctgctttgg tccaattgat attcaatgca
    21361 aatcaacgag tttcggtcat atcacatcaa caccaaaact tgcttggcaa aatttcaatt
    21421 ttgtaggaac gctaaagcaa tatttcaata ttccgattga atggacaaca gatgtcaatg
    21481 cggcttgcta tggtgaatat gttgctggtt ctggcaaagg gttagcaggt gttgcatact
    21541 tcactgtggg aacaggtatc ggaggtggtg ccttgttcaa tgggactttc gtggaagggt
    21601 tcagtcatcc ggaaatgggg catatgttga tcaagcgaca tccaaaggac aatttttcag
    21661 gaaactgtcc cttccaccaa gattgtttag aggggatggc agcaggacca gcgattgaaa
    21721 aacggttggg tgtaaaaggc cagaatttat tggctgacga ttcgttttgg cagattgaag
    21781 ccttttacct tgcccaatgt gcatacaata caacactgat gttttcgcca gatagaatta
    21841 tttttggcgg tggtgtaatg aaacaagaac acatgaagaa aaaagttcag gataaatttg
    21901 ttgagcttat aaatggatat gttgaaatac caccaattga tagctacatt attacgcccg
    21961 aattaggtga taatgccgga ataatcggcg gattagcatt ggccagaaaa gcggtaagaa
    22021 ataagcaacc ataattaatg aatgtttcat tgatactcat gtcaaaagta tttaaccgag
    22081 taatcaaatg atcaaatgaa gttggataaa gcgaaaatga attcaaataa aaaggctcta
    22141 agtcaaatcg tgttgttttc attaaatcac tgaaaggaaa tgcttctgcg tttccttttt
    22201 tctttttgtt gtgcgctgta taccttgagg atattcactg gtggcgtaca cttaaatagt
    22261 aatatattgt gttactttat gtctgtggta atagttatag ttgcttacca atgtatgttc
    22321 atttttcgaa atgttgactt cataatcctt ggcttaatgt ttgaattttt ttggtgatag
    22381 attttttcag ccgtataaaa taaatcgtaa ttctattata gagagttgtg attataaaaa
    22441 gatagcaaga aaaaaaaaga aagtttctat acattttaaa tattt
//
LOCUS       DACDHL010000041        22098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDHL010000041 DACDHL010000000
VERSION     DACDHL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.041"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            119..706
                     /locus_tag="IV228_001597"
     CDS             119..706
                     /locus_tag="IV228_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717495.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            706..1605
                     /locus_tag="IV228_001598"
     CDS             706..1605
                     /locus_tag="IV228_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP8717496.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            1605..2519
                     /locus_tag="IV228_001599"
     CDS             1605..2519
                     /locus_tag="IV228_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717497.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            2571..2786
                     /locus_tag="IV228_001600"
     CDS             2571..2786
                     /locus_tag="IV228_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717498.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            2976..3230
                     /locus_tag="IV228_001601"
     CDS             2976..3230
                     /locus_tag="IV228_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP8717499.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            3409..4458
                     /locus_tag="IV228_001602"
     CDS             3409..4458
                     /locus_tag="IV228_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP8717500.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            4635..4865
                     /locus_tag="IV228_001603"
     CDS             4635..4865
                     /locus_tag="IV228_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717501.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            4868..5125
                     /locus_tag="IV228_001604"
     CDS             4868..5125
                     /locus_tag="IV228_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717502.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            5342..6217
                     /locus_tag="IV228_001605"
     CDS             5342..6217
                     /locus_tag="IV228_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717503.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            6227..7021
                     /locus_tag="IV228_001606"
     CDS             6227..7021
                     /locus_tag="IV228_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717504.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            7050..8168
                     /locus_tag="IV228_001607"
     CDS             7050..8168
                     /locus_tag="IV228_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717505.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            8201..8521
                     /locus_tag="IV228_001608"
     CDS             8201..8521
                     /locus_tag="IV228_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717506.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            8776..9465
                     /locus_tag="IV228_001609"
     CDS             8776..9465
                     /locus_tag="IV228_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAP8717507.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            9502..9669
                     /locus_tag="IV228_001610"
     CDS             9502..9669
                     /locus_tag="IV228_001610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717508.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            9689..11317
                     /locus_tag="IV228_001611"
     CDS             9689..11317
                     /locus_tag="IV228_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717509.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            11368..11739
                     /locus_tag="IV228_001612"
     CDS             11368..11739
                     /locus_tag="IV228_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717510.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            11824..12024
                     /locus_tag="IV228_001613"
     CDS             11824..12024
                     /locus_tag="IV228_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717511.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            12158..13384
                     /locus_tag="IV228_001614"
     CDS             12158..13384
                     /locus_tag="IV228_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717512.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            14234..15559
                     /locus_tag="IV228_001615"
     CDS             14234..15559
                     /locus_tag="IV228_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8717513.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            15552..15899
                     /locus_tag="IV228_001616"
     CDS             15552..15899
                     /locus_tag="IV228_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717514.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            16250..17071
                     /locus_tag="IV228_001617"
     CDS             16250..17071
                     /locus_tag="IV228_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8717515.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            17091..17651
                     /locus_tag="IV228_001618"
     CDS             17091..17651
                     /locus_tag="IV228_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP8717516.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            18694..19461
                     /locus_tag="IV228_001619"
     CDS             18694..19461
                     /locus_tag="IV228_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8717517.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVES
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            19487..20032
                     /locus_tag="IV228_001620"
     CDS             19487..20032
                     /locus_tag="IV228_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717518.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            20033..20599
                     /locus_tag="IV228_001621"
     CDS             20033..20599
                     /locus_tag="IV228_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717519.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            20614..20862
                     /locus_tag="IV228_001622"
     CDS             20614..20862
                     /locus_tag="IV228_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717520.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            20882..21121
                     /locus_tag="IV228_001623"
     CDS             20882..21121
                     /locus_tag="IV228_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717521.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            21492..21773
                     /locus_tag="IV228_001624"
     CDS             21492..21773
                     /locus_tag="IV228_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8717522.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            21763..>22098
                     /locus_tag="IV228_001625"
     CDS             21763..>22098
                     /locus_tag="IV228_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8717523.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRK"
BASE COUNT         8441 a         2832 c         4465 g         6360 t
ORIGIN      
        1 tcactctttt tgtgtaaaaa aactttacac ttatttttga gtgtgttaag attttttata
       61 tagagtgacg gtttcttgtc agagtggatg ataaccatca atattggagg aataataagt
      121 ggaaaacaat aatgatatta gatattttag cattggggaa agagttacaa cagtaagtga
      181 attatcaaag gattatagag ataaagaagg aacgattatt tctcttaaaa agaatcgaag
      241 aggtgtttgg caatatgttg ttgatttagg ggatggcgtt acagttgagt gtaaagatag
      301 tcaactgatc agaaatgatg gcagtcattc cattgaaaaa gcaattcttg tattcccaaa
      361 agtatcaagc aagattatga atgactggtt gcttggaaaa tttggtgagt acaataaaaa
      421 agaaatcgca gaagtgatcg gcaaatctta tccaacagta ttagggtatc taaaaaagat
      481 aacgaacaaa gaagcgaaag ttgaggatgt tttaacagta agagaacttc agtcattaat
      541 cgaatatcgg aataaacaaa taacaaatcc tgagtacctt aaaaaagaag atggtctatt
      601 gaaacaattg gaacaggtaa ataaaatccg gaaaatgcaa aaggtagctc ctttaagcct
      661 agataacttt aaaaaaatct atttgaaaga gatcgaaaaa gactaatgtg gagtgtcact
      721 ttaccgtttc aacctgtatc tgcaccaaga ccaagtgcaa gaaggaatgt tgctgatgca
      781 actaatgacg tagcagagaa aaggatttca acctattatg ataaaaagta tctcgattat
      841 ttgaaaaaaa taaaaagctt tatcgaagat caaggtttgt tagatgatga attttactcg
      901 atcgtaaatg atccgatggg tgcaattatg gaagtggact tttactatca gataccaaaa
      961 agctataaaa aagtctataa cattatgaaa acgacagcgc cagatttaga taatttagta
     1021 aaaagtgcta tggatggtat tttcaacatc tcagctatac gagatagcca tatcacaggg
     1081 ttaattgctt ttaaatacaa tgtggcaaac gaaggaaaaa cagttgtgag gatcaagcga
     1141 tattcagaat ttgagtggga tacttctgaa ggattgaacg gagatatttg ggaagccaca
     1201 tttgatttca aaccagtagc aacgccaaga ccaaagtcta aatttagagg aaatgggatt
     1261 atcacttact atccaaaaga gtataacgac tatttggaaa atatcaagaa tacgcttaaa
     1321 gagaaagatt tggtaaatga tcaactgaaa aaggtaatga atgctcctat gggggttata
     1381 gctcagatag attattttta ccaagtgaga aaagataaga aaaagcttga ttcactgatg
     1441 cgtacttcac agccggatat tgataactta gtcaaaggaa cattggacgg aatttttaac
     1501 aaggaaattg gcatcaaaga tagcagggtg gtaggtttaa tagcttttaa atacaatact
     1561 tttgaaaagt cgaaaacaaa cgtgcgacta caggagatgg gctgatgaat aataacgaaa
     1621 aaattcttca tgtacttgat agctttgaaa caattcaaga ggaactaaag aaatatcgag
     1681 atgttttgga acaaagatat gattttgtca atcagcaaaa gagtaatcac atggatttca
     1741 ttctcaatat gaatgacttg aagaagaaat tagttgaacg aaaagaacaa gaaaagttga
     1801 tcaaagctta ctttgaactc ggtgaaaaag aagttaaaaa ggcaatggaa ctgaatgaag
     1861 atcgcagggt tttagatcag ctgttagaac aattattggt gatgtttcaa aaaggacgaa
     1921 tagatgagga tttaattgaa gaagggttga gaaagtatcc tgcaaactca ggtatcggaa
     1981 tagtgctgaa agcgattgat gaagaagaaa ttgaagattt cataccggca gaggattttg
     2041 aatcggcaat ggaatatata aaatattaca gccaagggat cactgctttt agagagtttg
     2101 atcctgaaga tgttattcat gacttgaaca acctaaaaga atggtgtgaa ggttatgaag
     2161 ttgatgattc cggtttggac tacttgatca gtattatgga aatcgaagaa gagatgccgg
     2221 ataagcctga tccaacagat attttagaat taatccatga tgctagaaat ccaattgcat
     2281 acatttctag aggatatact gtattggaat actataagcc ttatatatca gcgatgaacc
     2341 atttaagacg tgtattaagg gaaaaacgtg aataccgctc agtgttaaat gcatcgaata
     2401 gattagaaaa agctgtttca gagctagatg cttattatcg tgaacattac ctgcaagcag
     2461 gtggtatgcc aagaaatacg aaagcaaata tcagtaggta tataaaagaa gctgagtagg
     2521 cggagttagc ccataaaatg gcaaaaggta acctgaaaga ggcgttttga atgaaaaggg
     2581 atgtttttga attgtacgta ttagaacgtc tagaagacgg catgatggca caagagataa
     2641 ttgatgaaat tgatgatatg atcagcagat ttggtcaatt ggaattgaat gatggaaaca
     2701 aaaattcggt agtgctacaa caattttggt taaataattt ttcgtataag gattttatca
     2761 aagaaaaggc aaatagattg tactgactaa attcacgagg atcagtgatg agagcttatt
     2821 gctaagagca gttaggctct tttttgtttg tgttaaatta ttttatttta caatacacat
     2881 ttatacacaa aaaatgttga cggatagtac acaaatgtgg taggatatat ttgtaagcaa
     2941 gttagattaa caagttaaag gaaggaagag cagttatgaa aaaagaagaa ctaatagata
     3001 tgtttcaaat tgttgaacga gcaaataata tgggaattat gttttttgat cgtatttctt
     3061 taaagatgga tttaagcgtt gcccaccaag aatttaatct acgattaaaa gctttattga
     3121 tttctgatga tgttaatttc gctcatgatg ttgtaggtat acagaaccat atagatagag
     3181 aaaacaaacg tatgggtgat ggattcttac caagatattc aagtttatga tttggcgaaa
     3241 gattcaagag tatgtgtcat tattgatagg tactcttgaa ttggaaagga ggaatgaatt
     3301 taagacaaca acgattatga tcttaaaaca atcgtttatg taattggcaa tcttacgaat
     3361 aattttcatg caataatgac aataaaaaat gaaaaggggt aaaatcaaat gaaaaaattt
     3421 ttagtaggaa ttctattcac aatggggcta gtgctattat caccttcagt ttcagcagaa
     3481 agctctattt ccatgtatcg tatgtataat cccaatagcg gagagcatct atacactaga
     3541 agcgttggag aaagagacaa tctgaaacgt tatggatggc attacgaagg tgtcgcgtgg
     3601 caagctccaa caagtggttc cccagtttat cgcttgtaca acagatataa cggtgaacat
     3661 ttttacaccg ttgatgccgg agaaagagat aagatttcgc acacaggatg gacgtacgaa
     3721 ggtgttgcgt tctattccta caacggagca aatagagttc ctgtgacacg cttgtacaac
     3781 aaatacgtga attggcacca ttacacgtta gatcaaaatg aaaagaacgt aattagtaaa
     3841 caaggttgga agattgaagg tacgggttgg tatgcaatgc caaacagcgc accaaatcct
     3901 acacctgcac ctgctccaag taatgaaaaa gtgttaaatg ttcctgtcat ttggcaaggt
     3961 aatacgatgt tgtgtgaagg tgcttccctg ttagagggat tgcactacaa aggagtagcg
     4021 acaaatcaaa acttaaccag ttttgtaaac tatatgccaa gagctaatga caataatcca
     4081 tatcatggct attctggcga atggcgctac aacgtaaatg gtacgtatca aggcatgatg
     4141 cctgatccag tagttaaatg ggcaaaaaat gttggtggta atgccgttaa tatcactggt
     4201 tcaggaacga atgggatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt
     4261 acttataact atgcttcacc tcaattcaaa caaatgcctt ggggacgtgc agtctggaat
     4321 ggacatgtgg ttctcgtgga tggcttcaaa aatggtgcat accatgttgt tgaccctgta
     4381 tttggcgcaa aatggatcaa tgcaagcacg tatgagagag catttaatgt gactggaatg
     4441 gcggttgctg tacgttaatt gatagaatta ataaataaat gaaataagag cttaattact
     4501 gaaatggtaa atgggctctt attttttaga aaaaaactgt tttttgtaat acgaatttat
     4561 aacaaaaaac gttgaatgta gtacacaaaa gtggtatatt aaaaccgtag agattatgcg
     4621 aagaggtgat tttgatgaac caaagaagaa gaaaaaggct agaagaaata agcgagcgac
     4681 tatccgagtt aaaggatgag ttagaagtaa ttcaagaaga agaggaagag tacaaagaaa
     4741 atatacctga aaatctacag ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc
     4801 aacttgaaga agctgttgat ggtttatccg acatattaga attgattgaa gatatagcta
     4861 gctagagatg attaatgaag atttcaggag tgagcaaaaa atgattgata tttcactgat
     4921 agagaagcta cttttttcaa ataagtacac agccaaagag ttggctacaa gatggcaaat
     4981 atccgaatca gctattaaag cctatcgtca aaataaggga agcgtaggat ggcgtgattg
     5041 gaaaggaatc agcattggaa aagcagaaga cataatgaaa cgtgaaggat ttcaagctga
     5101 caatgattct aaaaaagttg aatagtttaa tagtcgccaa gaaaagtacc aggacgaggg
     5161 gaaaggtaga ttacaaagga aaacactttt aaataaactt cgcaaaagca tcaggttatg
     5221 agtggctagg gctatacgaa tgtgatgaaa acaaagggct ttaatgtcaa agaaaacgca
     5281 ataggggggg aacaaaattt ttaaaaaaga tatttatgtt tggtttaatg gaaaaaaata
     5341 tatgactact aacaaaaggt ggtcagagct agtacaaaat acagttaaaa aagtcatgcc
     5401 agatggatat catttcaact atatacaatg ctcacaaaca actgaaagta tttatttttc
     5461 aatattaaat gagaatagac tctattattt cagggtaagt tcacaccata ggaaaaagag
     5521 agaagataat gttacgacat ttttatcggg aaaatacaat ggcttcaaag agttaagtga
     5581 agccattggt gagtatctaa aaagtaatag atttgtcaga atttctgaca aacatatatt
     5641 gatcttgtta ctttatttga ataatagctt caaattgttt tggagagacg acactttcta
     5701 ctatattgag aacggcatgg atatttattc tggtatcaac ggcatattaa aacattatat
     5761 taaggcgtta cttcgatatg gggttcttta tcaagaagat ttaagtaacc aaattaagat
     5821 tagtaattct ggaaagtcat tactggcaaa atataaaaaa ttgctttctc ctatctacaa
     5881 aggaatagat atggaagctg taaattctga aactttattt aatttagttg ttgatgaaaa
     5941 gtttgcgaat gaatttttta aaagtaaaaa aattgaaaga ctaaataacc tgtttcctga
     6001 aaatgcaata aaagcttatg tagataataa taaatcacga ttatacaact ggtttattcc
     6061 caaacggttg cgaggaaaaa tcaaaattgg cgatcaagtg aaagtaatta atctaaatca
     6121 tactaagatc gttaccgtaa cggagttgta tatagctgaa aaagaagata ttacaaaaat
     6181 gaaaccagtt attaaaatac tagataacaa agagtgagtg gaatatatgg tagcaaaaaa
     6241 gaatactcta aaagaagctt ctgaaaaata tcaagatatg aaaagacgag gattaatacc
     6301 aaaggacaaa aaaggtatat ggagagaatt tgttaaggaa gtcagtagtg atcgaagtgg
     6361 cggtgacttt gttggacgat atacagcaaa aggttatagc aggcgcttaa ggatcactgt
     6421 tgaatatgga acgaatgaat cagctacaga gctgaagcaa aggtacttag atacacgaag
     6481 agaagcttca aaaaaatatg atgatttcaa gagcagaggt ctaattccta agaagaaaaa
     6541 ggggatttgg cgtgagtttt tcaaagaaga agatagcaat cggaataagg gagacttcat
     6601 tggacgatac acagcaaaag gctacactaa gaatttaggg atcaaggttg aatacggaac
     6661 tggtgaaagt tcatgggaat tgagaaatcg ctatttaaat acattgttag agaaatataa
     6721 agcagaatta ttggagttgt tcgatgttaa tgattttatc gagatggctg aaaagtatgg
     6781 tcatgaagaa ggttttatga tcaatgacat tatggttatt gataatttta cggatttatt
     6841 agcacttgcg aaaaaagcaa gggatttaag agaaacgcag atgtatctat tcagaaataa
     6901 aaacggaaga aaccggagaa ctgaatcgag tgagattgaa gattactatg atgcctttac
     6961 acacgatctg ggagcctttg tagacgagat aagtggtgta atgaacaaat atagggtcta
     7021 gaataaaaaa gccggagatg agcagataaa tgggaaacaa tagcatatac acacctgaaa
     7081 tgcagaataa aattaaaaga cttgtcggat tagggtggat gacaagagga aaattagatt
     7141 cttttagtac gatcaaatat cttttgatgg acatttaccg tcagaacaag caaacaggaa
     7201 tacatttttt gggctatgat aagccggaag atgcaccttt ctttgttcca agttcagcct
     7261 atagagccag tacctccggt agcatgtata acttggattt gtttgaggaa ggagcaaaga
     7321 tgatcgaata tgatttcaac gaagcctatt cgaggatcat gagaaattat cctttgccgt
     7381 caaacactta tttgaaagca aaatataata cagatcaagc tattttgaga gcgataaata
     7441 gaccaaaagt aagaccgtat gaaaatttaa aaacgtatat gttctttttg gttaattttg
     7501 aaggaacagc caaagaagga acatatgcta atccaaaatc acagtcgatg tttgcggatt
     7561 atggcgaaaa tcccaaaaca gctaatgagc ctatttggtt atcggagatt gagctaacac
     7621 ttatccttga ttattatgat attaaagaga tggaagtcct agatagccac acatttttga
     7681 cgaaaaaagg attactcaaa gaatattttg aaagaatcga gccattaaaa actgatcctg
     7741 atacgttgaa gctttataaa aatcttagaa cgaaagtatt tggtgctatc ggacaaaaat
     7801 cattaagaga aggagaaaca agcaccttcg ataaatacag tatgtataat cgagcgtatt
     7861 cctccatggt agcaggcgta tttagagatc gtatggttag atatgaacag aaatacgttc
     7921 atagcgcata tgggctatta aaaattcgta ctgatggatt gtatttcaga tccgaagtac
     7981 cagagtttga agagctgtat aaacttggag tagttaagaa aaaagttcat acaatatcca
     8041 aagaagaagt cgaaaaagct ttagaattta aaaacaaagg atttgaaaat aagggctcct
     8101 ttgggaaatc taaagatgaa gttaagggtg ttgtgataga cgatgatttc gagtttgatt
     8161 ttgactaaat actctttgga aaagatggta ggaggtaaat atgaagaaga aaattgtgga
     8221 aggaaatgta ggtgttatta ttctaaaaga ttcaactgac tatgctaaat caatggcact
     8281 aaaagacgaa aaagttgcac gcatggataa agatttagtt gaatttctga tggatgatac
     8341 agaaagatta aaaggggatc agtttttgag tttaatgaaa gacaaaggct taaacacata
     8401 tggtttattt gattataaga aattagctat agagtgggta aaagaaggag aaatattttt
     8461 tattgaagaa tgggaaggta ctgagaactt agttcttcat cagtatcttt tgaaagctta
     8521 atctaagtaa aacagttctg actagttatg cttgtcagaa tttcaaagga agttttccat
     8581 aaccatcatc attcaaaaat aaaggatttg aaaataaggg ttcctttggg aaatctaaag
     8641 atgaagttaa gggtgttgtg atagacgatg atttcaagtt tgattttgac tagatttttt
     8701 tgcaatgttt aagagtaaac aaggtaagaa ctaagatcat gaagattgag agtaaagaag
     8761 atagaggacg aatgtatgag aacacactat atttatttag aagataaaat tgtagatgaa
     8821 attccggcaa aaagtcgaga acaagctaga aaattattta ttgaaaaata caaagagatc
     8881 aatggagata gtgaagttcc aaaagtggtc gtaacagtta gaaaaaggca aacaaaaaag
     8941 agaagtacgt ttcatgtcag ccctgatata tataatctta tagagcgaca tagaagtgaa
     9001 gataatattt ctatctctca atatattgct acagttattg atgaattgat ttcaggaaag
     9061 tatgactatg cttttgattt tgagggtgaa aaggattaca aaagtgttgc tgtctttcaa
     9121 tctgattttg agaagctaaa aaactatgct ggcaggagaa atgttaattt tgcaagacta
     9181 ctagaagagt tctactccga agagttggga gtaggaaatg aatacaagag agaaacttat
     9241 gcgaaattgt ctcataagtt atcccaagcg cttaaagaag tgacagaagt taaagcggaa
     9301 ctagatgcta aacttgaaga agtgaaaaaa tatcaaacag agaggaagaa caacatgaag
     9361 aatcaagtgg aatttgacga acagaaagaa tttgaaaaag caagaaaacg ctataacaaa
     9421 attatgaatg aacttagcta tgaacactta acagttcatg aataattgag tgagggtaca
     9481 gaaaaatata cgatcgcaga tatggttaat gaagtggaaa ctcttttagc ttctttcttt
     9541 gaaaatggtc acgtaaacga tgaatggcga aaagaagaac ctaaaagggt taacgcttat
     9601 attgctcgat taaaaaggtt tattgataaa tataaggaga atagtgacaa attttctaaa
     9661 gataaataag gagagagtgt gattacattt gccagaaatc gaaggaatat tttcgccaaa
     9721 aataggtaaa aaacagttag atttcagcag ttttccgagc gtaaatagag attataatct
     9781 aggtgatgag gtacagatac taaatattga tttcaaagaa ctagacttcg ctgttgctgt
     9841 ttccaacaaa cccaaggcat tggaacgagt gaaaaaaata taccaagaga atgatcaaaa
     9901 tatcaaaaat atgatcaaaa agtatagacg agataattca aaagttgaag acgttttatt
     9961 caacggtacg gcaaaacatg atttgaatgt ttatatggca ttaggagtgg ctttgtactg
    10021 taattcaatt aatcagacag atattttggt taatatcgtg aggaatagct atcaaaaaat
    10081 attaaaaata agtagcgaaa caaaccctta ttatgcaatg aaatatcttg gggataattt
    10141 attaaatgat aaaaaattga tgcacaacac tgaagagctg ttatctaatt tggctgcgat
    10201 taactttcta ttcaaaacag gttctgaaga ttattttata gataacgtac taaaaggtgc
    10261 ttacggaaca gcattttatc cgctttacaa tatgaaacag cataaagccg aacaaaagga
    10321 taacaaactt gaaattaaaa agatgaaaga actattaggt ataggatttg taaaacccaa
    10381 ttcaaaagtc actattgctt cgttagcaac tactaacaat gatgggaaaa tagtaaatgg
    10441 gtttgacgat tcagatatag atatattgtc aaaatcatat ttttctttgt atggagattt
    10501 tgcagatttg atgggtattg atagttactt agtcaataat tttgagatca agggtagcga
    10561 gctgaatgat attgtgtatt tcgttttaat ctctctatat gcaattgatc agaataccga
    10621 gtaccgacaa attgactatt tgctagtttt ttatagtttg ttatatcagt tcgttttaac
    10681 tcggttgttt aaaaatgcta aggaagaatt gatcctgaat tctgattatg aaatagcaaa
    10741 acaaattgca agtattagaa aagaaaacaa atctgaagta atcaaattaa gaaaagaatt
    10801 aagtattgcg agtaaagaaa acggtcggtt agaatcttta gtcaaatcgg caaaaaaaga
    10861 agaggaactt ttgaagcaag agattaaaga attaaaagga atcagaaaag aacttgaaca
    10921 gctgaggttt gaaaataaag tattaaagca attgcaggag caagaacata gacaatcatt
    10981 aaatggtaaa actgaagagc cttcactgtc tgaaatgata gcttatctaa aacataaaaa
    11041 cattctttta gtgggaggac atgtaaattt tcaaaataaa ttaactgaac tattaccaaa
    11101 tattaaaatg tttgatgtaa gcgaaaataa tggaaagata cttaaaaatt tgaatacgtg
    11161 tgatgcgatt tttctatata cagattactt gaatcatggt ctatattatc gtgttatgag
    11221 tgaaatttct aataaagata agatatttta tttggataat catgtgaata tgaacttaac
    11281 aattaaacgt atttacgatc ttttatcact tatataaaga gagaagtgat aaataggaca
    11341 caaaatttgg aaagtaggta agtcactatg agagaactaa ctttatcttc taaggaatta
    11401 gaaaagaaaa ctgctttatt aaacgaaata tttagtgctg attgtataat ccaaagttta
    11461 aaagaaagtt cattttcaaa actaacagat caggaaaaaa tttcttatat gctaagttta
    11521 gataatctag gaactaaata tgatgaatca gcaagtagcg ataagctttt gattgaatta
    11581 gtatcaagtc ttagtagttt tggaaaaatg aaaagattta atgtgatatt agaaattttt
    11641 gcttggttaa tcccttcatt tatttggttt ttttatattt tcatagttga atctgagtta
    11701 atagccaagg tctctcttgg actgttaatt ccgatataaa taggttcagc aatttacttt
    11761 aataacttgt atacacgtat tcaaataaaa aagcagttat tacatgaagg attaagtggt
    11821 aatatgttaa aaagaattgc atgggataaa gataataaaa cagatattga gcttaaagaa
    11881 tataaataca attatttctc gagatgtata gatagaaagt tactgtttaa aaaagaagga
    11941 ttagaattta gttttgatga agatgaattt gaaaaattag tgatggactg gcgtaagaat
    12001 ttactggata cggtacaatt ctaaaagcaa ttgaggtcga aattccgata cttgaatcat
    12061 gatctatatt atcgtgttat gagtgaaatt tctaataaag ataagatatt tcatttggac
    12121 agtcatgtga atatgagctt aacaattaaa cgtatttatg atcttttata tgtttataaa
    12181 cattaatgag gtgctaaaat tgacaacaat aactatgaca aaaaaaatag aagtatatgt
    12241 tgatggatca caagaagaaa gggattcctt ttttgatttt ttttggaaaa atagtagaga
    12301 atatgtaaaa gcgttgaatc tggtatataa acagctaatg tttgaagagt tgataaaaga
    12361 tgcaattaaa gaaatggatc aaggttatgc tgagagagaa gaaagtattc aaaatcggat
    12421 tgaaaagtta gcatatactg atccaataga tcaaaaaaaa cttgataaag caaaagctga
    12481 attatctaaa ttgagaaatg aaaaaaataa agaagcgaga gaaatattaa cgcaagcgat
    12541 agggttgaag agtcaaacga gaacacgaga tgtagcaaaa aaattagact ttgactttgg
    12601 agattttgtt gattccgcaa atatgaaagc tagtaaagat tttggcaatg attttatcgg
    12661 aattgtgacc ggacaaagaa cgcctagaat atataaaaat agaaacaaat atggattact
    12721 accttttaga ggtcgagata ttaaaatcca taaagatgat aagttttata tttatttacc
    12781 taaaggcttt aggtttaaag tgaatttagg tagcaagcca agaaaagcaa gagatgttgc
    12841 ttcagtttta caacacatta tggatggcga ttacaaatta aatcaaagta atcttatttt
    12901 tgatggaaga aaattatttt ttaatctaac aatttctttt gaaaaaagct ataatgaaat
    12961 ggaattcaag aaaggtaatg aattgatcgt aaactttgca gatttgtgta gcggtgaatg
    13021 tttaattaat gataaatctg taatgtcttt tggtgatagc caatatataa gaaacttcaa
    13081 aagtaaaatt ttagcaatga aatcaaaaga acaaagccga ggagtctttt ctaagggcgg
    13141 tcatggtcga aaaagaaaat tagatcaaga tcgatgggaa gcaataaaga aaagagaaag
    13201 taattttgtt aaaacttata ataatcaatg ttctgcaaaa atcgttaaaa ctgctattcg
    13261 caataaagct caaattattc gtatcattga accagataag gcttatggcg aatgggctta
    13321 ctatcaattg aaagaacaac tagtaaacaa aggaaaaaga tatgggatag aagtcagaag
    13381 ttagtaaatt aaacaatcgt tgatttggca acgaaatagt gagggaaaat ccactcactg
    13441 aagggtaaac tccgtcaagt tgtgtgacca gacggaagga cgaatgttgt tgaggtttta
    13501 gaaccatata aaacagcatg tttctcaaat ttttaggata agggaaattg ctagcagttt
    13561 gttttagaac catataaaac agcatgtttc tcaaatgagt actttaaatt aattgatggc
    13621 gttcctgctt tagaaccata taaaacaaca tggatttaaa acaagaagta agtcagttct
    13681 ccttttactt tagtaaggaa aaataaagta ttctcaataa aacttaaaat aagcaaacct
    13741 ttgttttaat agctaatcga agtaaaaccc aaagccatat gaacccctga atcagcgaaa
    13801 gtttatgatg atttggatta ctaatagttt aggaattaga tggtaaatct aaattaaaca
    13861 atcgttgatt tggcaacgaa atagtgaggg aaaatccact cactgaaggg taaactccgt
    13921 caagttatgt gaccagacgg aaggacgaat gttgttgagg ttttagaacc atataaaaca
    13981 gcatagttct caaattgttt aaaatgattg tcaagttaaa aaaatctggg atcatataaa
    14041 atagtatagt tctcaaatat gattagaaac cctattaaag agcaatatta agccaactta
    14101 cttaaatcgt ttaattaaga acaataatct cttttagtca gacttttagt ctgacttttt
    14161 tgtatttcca aaatttattc tttacaaaaa cgaacatacg tttgtataat tgcttgtgaa
    14221 tgggggaata aaaatgtttg attacagtag agaaccggtg aatgactatt ttttaatcga
    14281 catgaagtct ttctatgcta gcgtggaatg tattgaacga aatttagacc caatgactac
    14341 accgttaatt gtcatgagta gatcggacaa cactggttca ggtctgactc tagcttcttc
    14401 acctactgca aagaaaaaat atcatattac taatgttagt agagctactg atttacctta
    14461 tccattacca aaagaacttc atattgttcc accacgaatg aacttatata taaaaaaaaa
    14521 tatggaggtt aatcagattt ttagaaagta tgtcgcagat gaagatttat tgatttattc
    14581 gattgatgaa tcaattttaa aagtgacaaa atccttaaat ttatttacta gttcaggaac
    14641 aagaagcgag agaagaagag aattagctaa aataatcaaa gatgagattc agaaagaaac
    14701 aggtctaata gcaacagtcg gaataggaga taacccatta ttagctaaac ttgcactgga
    14761 caatgaagcg aagagtagta aagaatttat agctgagtgg acttatgaga ctgtacctga
    14821 aaaaatatgg aaaataaacg agatgactga cttttggggc attggatcac gcatgaaaaa
    14881 aagattgaat catatgggaa ttatgagtat agaagattta gcaaattgga atccctacac
    14941 aattaaaagt cagttaggtg taatcggact ccagttatat ttccatacca atggaattga
    15001 tcgtacggat attgctattc caccagaacc aaccaaagaa aagtcttatg ggaattcaca
    15061 agttctacca aaagattata cgaggagaga agaagtagaa ctagtagtca aggagatggc
    15121 agagcaagta gccattcgaa ttagacaaca caattgcaaa actggttgtg tacgcttagc
    15181 aattggtacg tcaattttgg aagaaaaatc aggtttctcg caccagatga aaataccaat
    15241 tacagataat acgaaagaat tacaaaatta ttgtcttttt ttattcaata agttttatga
    15301 ggggcaggaa gtacgacata taggagtaac ttatagcaga ttagtataca cagattccct
    15361 acaattagat ttatttagtg atccaagaaa aaagattgct gaagaaaatt tggataggat
    15421 tattgataag attagacaaa aatatggatt tacagctatt atacatgctt catctatgtt
    15481 agaaggtgca agaagtatta cgagaagcac tttaatagga ggacacgcag gaggattagg
    15541 aggtttgcat aatgattgaa tataacgata gaggacgttt aaaatggtcg ggattttatt
    15601 tgtctgatca tactgaaaga atcaatgcag acaatgtaca acgtgctaat cataatttgg
    15661 caaaagaaca aatgacgact gaagagattg cgaaaatact gagaactgct gtcataaaaa
    15721 atagacccgt ctgcattcaa aaagaggaac gaaacgctga aggttactat ccagatgatg
    15781 tagtaggaaa aattattgga tatgacgagc ttggtgtatt tgtggaagaa gaaaaagttc
    15841 actatgatga aattaggaat gtttctttct cggaattatt aaaatggagt cagatatgaa
    15901 cattattaaa aaatacgaat tcggttatat tacgtatgaa cagctaatag aggaattatg
    15961 gggatatggt caacaattaa ttaatgaagt ggggattgat tgcttttgtt tctatattga
    16021 atccggagca ggataccata agaacagata ctatatattt ccatatccac caaaatagtc
    16081 tcttagatat ttttaaattt tctccaaata ctttcaatta ttgtgaaata tttcccttta
    16141 attaatcaaa ttttttataa aagtaatata taaaataaat tgcttgaagt attatattta
    16201 gatatcataa tttatggaag aaaaattaat taaaaaggat gagattataa tggggttact
    16261 ggttccgaga gctaatactt atactatacc tttaacgaaa cgccaattaa ttgctaaaag
    16321 atatcaaagg attactagag ccatcaatag agagttttgg aattctgaaa gtgatactgc
    16381 tcacagttta tacgtaggtt cttatgggag aggaacggca attagtacta gtgatataga
    16441 tataatagtt gaattaccga tggcagaatt tgacagattc aaaaattatt tatcaaatgg
    16501 cccgtctaag ttattacaag ttataaaaaa cgcatttcag gaaatactac caaatagtga
    16561 tattagagct gatggacaag ttgttaaaat aaatttccat gatggaatca aatttgaaat
    16621 agttccagct tttaatgaaa aagattattg gggtgagtca aaagggttta tttatccaga
    16681 ttcaaacatg ggagggaact ggaaagctac aaatcccaaa aaagagcaag aagccatgaa
    16741 attaaagaat acaaaaagta ataatttatt atatgctacg tgtaaacatt ttcgacatgt
    16801 acgagataca gaatttacga gttatcatct ttcaggaata gtaattgaca gtttcgtata
    16861 tgaggctatg ggaaattgga aattcgttga aaataacagt gggggtcaaa acattagttc
    16921 tgtatcttat gagacagctt tattagaata ctataactca cataaagtta tgggcggttt
    16981 aaatttatac tctcctggaa gcaatcaatt tgttaattct gatagtagta tcatatgttt
    17041 agaaaaagta ttaaaaaaaa tagctttata aagttttatg gaggaacaac atgggagacg
    17101 aaaaaaagcg cttggaagtt caattacgtg aagcatatgg acgagtaacg tatacttaca
    17161 ccactcattt aaagcaagcc gataatatta tttctgacaa taaaatttta aggtgtcttc
    17221 aaattggttt gtcagccctt actactggag gggtacttag taccattatt actgataata
    17281 gtgagttaac ctttgttgct ggattatgtt caacaatttc tcttggatta aacttatact
    17341 ttaagaattt tgatcttgct gaagaagcta aaaatcatca aattgttgcc gataaattat
    17401 ggttgatcag agaagaatac gtaagcttac taacagattt aaatcgttta agtgttgaag
    17461 atattgtgaa aaaaagagat caattaatga acgaaactca tgaagcatat aaaaatgccc
    17521 ctaaaacaaa tagtaaaagc tatgaatcag ctcagaaagc attaaaatct gaagaagagc
    17581 aattttttac agaagaggaa ttggataaga tgttaccaga gcatcttaga aaaaagagtt
    17641 ctaaatattg aactcttttt ttatgcttaa aattctcttg taatttttct attcatctat
    17701 atttaagaag aaatatgatg atagaaacat ctcctaaagc caccgcaagc cgttctaagc
    17761 atagcttagc ccgacattgc tagatatgct catctcacca tgaacgtttc ctaaagccac
    17821 cgcaagccgt cctagccatt gcctagtccg acatcgctag attagtgcat ctcgccataa
    17881 aagttttcta aagccaccgc aagccgtcct agccattgcc tagtccgaca tcgctagatt
    17941 agtgcatctc gccataaaag ttttctaaag ccaccgcaag ccgtcctagc cattgcctag
    18001 tccgacatcg ctagattagt gcatctcgcc ataaaagttt tctaaagcca ccgcaagccg
    18061 ttctaagcat agcttagccc gacattgcta gatatgctca tctcaccatg aacgtttcct
    18121 aaagccaccg caaaccgtcc tagccatcgc ctagtccgat tttgcttgat cggttccatt
    18181 tctgtttatt tagtgggtca acaccgtaat cttaaatagc catcgcctat ttaagattac
    18241 taggtctgct ttgaaggtaa aaagtctttc attcttcaag acttttaggc tttcaagcta
    18301 cgcctgaaag cattgaaatt catcgctctt tatttggttt tgccatttat aattccttac
    18361 gcccattatc tttttcgcct gtcatacctt gtcagaagtg tttagagctt atctcatctt
    18421 tggattaata caagtggcgt aaattttttt ctaaacgcaa gcgttacgaa aataatttac
    18481 ttaaacactt gtattaatcc ggcagataag aacgcattct aatcactccc gacaaggcaa
    18541 gacaggcgaa aaagataaat aaatgaacga aaggaagaaa cataaaatgc aaaaccaaat
    18601 agcgatgaag aaaaaaccac ctccctctaa aacaatccaa acaaaaaaga attttgaaaa
    18661 accaaaaatc aaaaacagaa agaagcgttc aacatgagag atagaatttt agaagttaat
    18721 caagaaatgg taaacaagtt aacaaatatc agtgggaact atgaaaagta cgatggtact
    18781 ttacttgata actatatttt tcacgataca caaaatatca gaattggcag aagtaaaccg
    18841 agagcgttca ttctcatcaa agagaaatat ttgaatgaat ggaatagcac ctatgaaatg
    18901 gttatgactg ataactttga aaaggtattg gatttcagaa atgctttgtt gggttctgat
    18961 atgggaaata gcagtcttga atctattgaa gttgaaagca agaccgagaa tgaaggtgaa
    19021 ggattgatta agaatgtctt gaaaatcgaa caaaaatata tcagtgaaga tagaagtttt
    19081 tatagagtca caagctcggt tggtctagca aactttgcta ctaacttaat tggaaacaat
    19141 gctagtgaat ctttgctagt gatgtgtctg aatacaaaga atgaggtcat tgcttatagc
    19201 gaagttttca aaggtggaat taatcaatca gtagcgatac caagagagat ttttcaacta
    19261 gctttgctaa acaattcagc aaaaatcgca gtatcacata atcatccgag cggatatgaa
    19321 tttccaagtg aaaatgacaa aagttttact acgaatttga aaaacgcagg aaaaacaatt
    19381 ggtattgaat tgttagacca tatcatcgta acaagtaaaa acacaagcta ttactcattt
    19441 caagaaaatg gttctttata aaaattagag aaaagaggaa aacaagatgg gcgaaagaac
    19501 acaagcgtta attacagtca aatatggtaa cagcaaaacg aaagcaagcg tgtcgattca
    19561 ttatcagtgg gggtatggtc gatatatgct gatggacgta ttgaatcaag gaatttattt
    19621 aatcaataaa gcatacgact tacgagataa atcaagagaa gaattagcaa aaggaatctt
    19681 tgatagaagt acaggagtta tcaattttgt tacctatgaa tctggagtga gtgcttatga
    19741 tgaaacaggg tttggtgggc gctatgaacc ttatcaagaa acgattgaag atgtgatgaa
    19801 gttttcagat aacaatgatg gctatgctca tttagagatg gtgttcgatg aaaatggatt
    19861 tagtctagat gataaaacca gattgcgatt gtttgatttt gagggtcaac agataggact
    19921 aaacaagttt tgtgaaagcg gaaaagagtt tgctacgaaa gattttatta agggatacag
    19981 attattgcta gaaaattatg gaataaacct agaagaagag agcgtgaaat aagtgtttga
    20041 aaaaagaaaa gaccagttcc ctaatttaga agcgaaaagt caaaaagtga ttgatggtga
    20101 aatgattgaa ttattccaaa aaagttatgg aaccatagaa actaccgcaa gttgcaaata
    20161 ctactttcaa ggaaagagaa acacgtttaa aggctttgcc gattctgatg aagaattggc
    20221 aaagaaaatc attgattggc ttgtcagcag tgaacacgta gcaagggaag ataccgttag
    20281 aacaacgatt aatggggaag tcctaacagg tactagatgt gtttgttgta atcatgaatt
    20341 tgtagaagat gaaggttgct accgaatcaa tgaatatggt gattttattt gccagagttg
    20401 ccacgagata gaatttccaa ccgaaagaag ttggtttgaa tacatgctac aagattatga
    20461 gatggaaaaa gaaaatggtg aagtagtttt ttgtaccaaa gaggaattga gtaagagaag
    20521 cgatgatgaa attgaagatt ggtgtgtgtg gttagtaggg gcagatgatt gcgttgcata
    20581 ttacacaaca gcagaatagg agtgtgaatc agcatggaag gaatggattt tctagatcat
    20641 gaagatttgg ttgattttgg ttatacatgg aaaggaatgg taggtattag cagaagtctt
    20701 gctaatgcct tttacgaaag aaattatgca gtatatgttc tttatgatga cgatacagaa
    20761 agccttgttg acgaggaata taaattggat ttagagaatg ttttatatgg aatagaaaag
    20821 gaagatttag caaaatatat tttttcatgg ttgggtcaat aagtgaaagg aagatagaaa
    20881 catgacaaaa tttaaaagta agggtatgca atggtgttct tattattatg ttggaaatga
    20941 tgaagattta ttgagaaaaa cccaacgaaa aggttatcca gataagatat tgaaacaaat
    21001 aggttattac atgccagaag agaggataga aaaagaattt tgtgaggttc tttttgatag
    21061 tgaacagcta ttagttttgt taacccaaaa taaattgatt tgccaatttg aaaataatta
    21121 agatgaggtg gtgaaccatg aatgaagata agatggtatt aagtgaatat gctagattga
    21181 gaactattaa ttctttagca tctcatttaa gttgttcttt cctaacaaag gtgctaaaat
    21241 taatttacaa atgtgaagat tttgtacaag agaagcacaa agacaagcct actgcgaata
    21301 ggcttgtctt tttttcattc attacgattc cgtatgtgtg ggttagcgac aatttgttca
    21361 attaccgtat gatatagcat cttaatttga ttagattact tgcaatgtag tttattaaga
    21421 gtataatata atcataaatg tggacaattt gataaataat gagtgtttta tactcaaaag
    21481 gagtggatga aatgaaggtc aaaaatccaa ctgaaaaaag cagaatccaa gtcggaatag
    21541 acaagaattt aaaagagaac gcagaaatga tattggaaga gctaggttta aatcctacaa
    21601 cagcaataac aattctttac aaacaagtag tcgctagagg tgagtttcca gtagagataa
    21661 agctaagtga agaggagaag caaggaatca gattgcaaca gctaactaag gacatgccag
    21721 tagatgttct agatacagat gaaaagttag aagagtggtt cgatgaagcc taatgagata
    21781 gtaacggtat atgttgcatt tacagataaa aatggtggta aaagaagacc tattttggtg
    21841 gttagtgata aagaagatag agtagaattc tttggaatca caagtcaata tgagaaaaaa
    21901 tcagaccaaa taaagagggt gtattttcct attgcagaat gggaaaaagc aggacttaaa
    21961 aagcaatcat ggattgacgt agggtcttta aaggctattc ccaaaagccg agagaacata
    22021 tcattcaaaa aggttggtat gttatcagct agtgatacga aaaagctaaa taggtttatc
    22081 gagaaattac aaagaaaa
//
LOCUS       DACDHL010000042        21432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDHL010000042 DACDHL010000000
VERSION     DACDHL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.042"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            406..1062
                     /locus_tag="IV228_001626"
     CDS             406..1062
                     /locus_tag="IV228_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717524.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            1241..1806
                     /locus_tag="IV228_001627"
                     /pseudo
     CDS             1241..1806
                     /locus_tag="IV228_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2505..3026)
                     /locus_tag="IV228_001628"
     CDS             complement(2505..3026)
                     /locus_tag="IV228_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8717525.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            3224..6904
                     /gene="nifJ"
                     /locus_tag="IV228_001629"
     CDS             3224..6904
                     /gene="nifJ"
                     /locus_tag="IV228_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8717526.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            7093..7980
                     /locus_tag="IV228_001630"
     CDS             7093..7980
                     /locus_tag="IV228_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8717527.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            7977..9146
                     /locus_tag="IV228_001631"
     CDS             7977..9146
                     /locus_tag="IV228_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717528.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            9170..10525
                     /locus_tag="IV228_001632"
     CDS             9170..10525
                     /locus_tag="IV228_001632"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8717529.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(10597..12402)
                     /gene="glmS"
                     /locus_tag="IV228_001633"
     CDS             complement(10597..12402)
                     /gene="glmS"
                     /locus_tag="IV228_001633"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8717530.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            12812..13102
                     /locus_tag="IV228_001634"
     CDS             12812..13102
                     /locus_tag="IV228_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717531.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            13115..14140
                     /gene="sppA"
                     /locus_tag="IV228_001635"
     CDS             13115..14140
                     /gene="sppA"
                     /locus_tag="IV228_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8717532.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            14143..14802
                     /locus_tag="IV228_001636"
     CDS             14143..14802
                     /locus_tag="IV228_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8717533.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            14959..15891
                     /locus_tag="IV228_001637"
                     /pseudo
     CDS             14959..15891
                     /locus_tag="IV228_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16045..17334
                     /locus_tag="IV228_001638"
     CDS             16045..17334
                     /locus_tag="IV228_001638"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8717534.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            17581..18831
                     /gene="clpX"
                     /locus_tag="IV228_001639"
     CDS             17581..18831
                     /gene="clpX"
                     /locus_tag="IV228_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8717535.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            18914..19501
                     /locus_tag="IV228_001640"
     CDS             18914..19501
                     /locus_tag="IV228_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8717536.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            19866..21194
                     /locus_tag="IV228_001641"
     CDS             19866..21194
                     /locus_tag="IV228_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717537.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            21205..21414
                     /locus_tag="IV228_001642"
     CDS             21205..21414
                     /locus_tag="IV228_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717538.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         7054 a         3664 c         4626 g         6088 t
ORIGIN      
        1 ggagacaact gaatgaagat tttagttttg tacataaatt tttgcttaat atagaagtcc
       61 tgatatagat aagatcaagt atttatctat aactggcctg attaattaaa aaactggaag
      121 gaactgctcg atcatccttg agaactgctc ttcttacttc tggtcaagat tctcgtggtt
      181 ggtggataag gatggttgat aagttcattg gattagctag tcgaaaacaa ccaggtgtaa
      241 ttaatgtaga agttttaaca aggggatgga gtgatttatt cagtgataat cataagttca
      301 taaatagtaa ttgagatgta tggttggaat acgttatacc tgaacttgaa ctaccaagat
      361 ttggtttaga atttgagaat cgatgtttat aaagggaggg caaaaatgcg tttttttaca
      421 acttttacta tcattatgat tgcagtactt tttatttttc tagatatagc aaagcgaaat
      481 acagcatttt tattatatcg ggttttactt cgagcaggtc tcataacatt tatatcgatc
      541 gttggatttt ttctattcac agttattgta tttatttgga gaactccagc accaccattg
      601 ccagagatta catatggaga atttcctttt agacttgagt atgaattgaa tgaagaatta
      661 catgtgatag aagacacgtt aatagttgaa tttgatggat ttggaatgaa cgaaggcata
      721 gggagatatc gtagatggac aagtcgttta gccagcggag aagatttagt gttgctgtta
      781 gaagttagtg ataataagca aatattttat tttccaggac ctgctaatta ttacatggga
      841 gataggttga atggttataa tcatactttt cctagtgctt ctttcattga aagggaaaga
      901 ggcattacta gaagggatat tttacatgac aaagaattat tagaacaatt tggtccgctt
      961 gatcaaaata caataaatga agaagaattg ttaaatcaat ataatataag gctagtgaat
     1021 tgggaaatat ctgaaccgat tgttaacaat tttggtgatt agaaaacaat tacgataaat
     1081 agaaacaagc caaatcatca gcaagtaaaa gctgatgatt tggcctttct tatacgagta
     1141 gtaggaattc atagcgaata ttattacgat cagtagatat tttgatatta aaggagtatt
     1201 acgaactagt atgtgagaat gtagaggtga tttgcgaata atgagaaatt tatcaaatag
     1261 aaacaaaata ttaatcatta ttgttgttat agctgtgttt catctgggaa cgaatgcggt
     1321 tttaagtaga ataatattgg gtcctaagcc accaagacct gcgattaccc gaggggagtt
     1381 tgattttaga ttagaatatg aagtagatgg ggaaaggatt gtgatagaag atacaattgt
     1441 tgcattattt gatggatttt ctgcagatgc aggtagcatg gcatggtatc gcacgtggcg
     1501 tttgcatttg gcgagtgaca agagggcaca acaattattg ttacatgagt tagatgatgg
     1561 tcgaaaaata tattatcatt ttggaggagc tggattttat atggggaagt tagtagcagt
     1621 gtaactgtaa caaggaaaga tttgccaggt cttggcattc gaaactccat attttatatc
     1681 gagcgtggga gagatacatc tattgggaac aggaaagggt taactcttga agagttatat
     1741 aatgactttg gaatccgtct tgttagttgg gaacacaatc caccaataga aaatcgcttt
     1801 gaataatcga aatctattaa tccatttcat cagccagtga atgttgtaat ggaacaattt
     1861 ggtttagtgt tcaaaaccat tgaaatacta ggctgtgatc agcaaaaatg tatctgaata
     1921 aaaaagagag caactattaa atgagaggag ttaggagttg ttagctttat acgaatcaaa
     1981 gtggcaaata cagatgcgta tgcatttgtg tttgccatct tgcgtttggt agacgagata
     2041 ggtataagct aaagaaacaa cttgtttaaa ataaacgacc tagctcaaca aacgaccatc
     2101 taatcaacta acagcataca taaaagcaac tatttttgaa gtagaattga aatcgggata
     2161 acttaaaaag tgattgggat actagtgtga atcaccgtac ataaatatta ctcgttaagg
     2221 gaatttgaga aattttgtat gaaaatttaa gaattagtta gtatgcgcaa gtcaaaaata
     2281 tgaataaatt atttggtgga gataatttgc tcagcttgtc ataagaaatg agataatctg
     2341 gcagatgcaa gcttttggac accatttatt cggaataagg aggctgggac agaagcgttt
     2401 aactccaaga aataagaagg agttgcttct gcttccgccg tttataggtg tttagagcgt
     2461 gagacaaaag tgttctttac ttttgtccta cgctccttat ttttttattt tgtttggatc
     2521 aattctttta attcctctat tgaaatagat tcatcaaaga tttctttacc caagatgaca
     2581 gtcggaacaa atcgaatatg tgcttgctca gcttcgttga tgacagcttg gctcagctgt
     2641 tcgtcttttt gttctgtcag ctttagttta tctactgcaa aatccgctac ttcttggaga
     2701 gaaagatgtt tccactcgtc ttgagtatcg aatatttgtt tgatctcttt gatcgctttt
     2761 tgaccatcgc tgattgtgag ataccggtgc atcacgtttc ctcgctgcaa gctttctttt
     2821 tctttatcga acaatttgat cactcgctgc aattgcccgc tttggactgc ttcttctaat
     2881 agctcataag attcttcaaa ccattgtcgg caatacggac aagataggtt gatgaattcg
     2941 accatttttt taggagcatt cgatgaacca taaaggattc ctttttgtgt atttgttttc
     3001 gttgcatcaa tcactgatat atccatgtta aaacacctct ttcgcttata gtttatacat
     3061 gtctgacaaa aaaaggcaac gatttagact gtttgtctaa aaattagatg accaattcaa
     3121 atagacatat gtgaaatatt tttataaata aaagaaaaaa tggaatgttt tacgttataa
     3181 tggaacagat aataagagag aatatgaaca aggatgatga atcatgcgaa aaatgaaaac
     3241 aatggatggc aatacggcag ccgcatatat ttcttatgca tttaccgaga ttgctgcaat
     3301 atatccaata acacccagtt cgaccatggc tgaattagta gatcaatggt cagcagcagg
     3361 aaaagaaaat gtattcggtc aaccagtaaa agtagtagaa atgcaatccg aagcaggagc
     3421 agctggtgtg gtccatggat cattgaaaac tggtgcactg acaactacat ataccgcttc
     3481 tcaagggctg cttttgatga taccgaatat gtacaagatt gctggggaac tactgccaag
     3541 tgttttccat gttgctagtc gggcagtgac gacaaatgcg ttgaatattt tcggagatca
     3601 cggagacgtg atggctgcaa gacaaacagg atttgctatg ttagctgaaa gcagtgtaca
     3661 agaagtgatg gatttagcac ctgttgccca tcttgcttct attgaaggag aagtaccttt
     3721 tttgaatttc tttgatggat tccgtacgag tcacgagatt caaaaaatcg aagttttgga
     3781 atatgaagaa ttagctcctc tgatcaatca ggagaaatta gcttcatttc gtaaacgagg
     3841 gatgaatccc aatcatccga ctgttagtgg tacgaaccag aatccagaca tccattttca
     3901 acaacgggaa acgatcaacc cctattatga taaattacct gaaatcgttc aaaaatatat
     3961 gaaagagatc aaccgattac gaggaacttc ttatgacttg gtgacttatt acggagcaga
     4021 cgatgcggaa gaagtcattg tttctatggg atcagttgca cagacgattg aacaaacgat
     4081 tgattacttg aatgcaaacg gacggaaaac aggttttttg aatatccatc tttatcgtcc
     4141 ttttccagtg gaaacattcg tgcaaaagtt gcctcataca gtaaaaacaa ttgctgttct
     4201 tgatcgaagt aaagaaccag gggcaggtgg agaacctctc ttattagacg ttcaaagtgc
     4261 gttatatgat tctgaacttc gcccggctgt tattggtggt cgctacggat tggggtctaa
     4321 agatgtgact cccgaccaaa tctcagcagt ttttgatgaa ttgaaaaaag acccaagcat
     4381 acgcaaaaaa cggtttacca tagggatcgt ggatgatgtg acttatcaat ctttggaacc
     4441 aaaggaaagt ctggatttga cagaaccgca aacattccag gccaagtttt ggggatttgg
     4501 atctgacgga acagttggag caaataaatc agctatcaag attattggag atcacaccga
     4561 taaatatgcg caaggctatt tttattatga ttcgaaaaaa tctggcggtc tgactgtctc
     4621 tcatcttcgt tttggagata cgccgatacg ttcagcgtat cttgtcgagc atgcggacct
     4681 tgttgcctgt cacacaccag cttacctgca tagctatgac ttggtcaaag gattgaagcc
     4741 aggtggcatt tttttattga atactctttg gagcgacgaa caattagaaa cgcatctgcc
     4801 cttaaaatta aaacgttact tggctgaaaa taacattaga ttctatacga tcaatgccat
     4861 gagattggcc caagaagtcg gtttgggacg ccggatcaat acggcaatgg aaacggcttt
     4921 ttttaaatta gctgacatca ttccttttga tgaggtgctt ccgctgctaa aagaagaagc
     4981 cctgaaaagc tatggacata aatcaatggc tatcgtagaa aaaaacatac aagcaatcga
     5041 tcggacgacc gaactgcttc atcaagtcgc gatcccagac cattggagaa cgttggagat
     5101 gcctgaaaaa atcaataaaa ggaacactgg ttatatacaa gaaatacttg aaccagtgaa
     5161 tgcacaggaa ggaaatcaac tctctgttgg tacacttgta gcaaatggaa tgacaactgg
     5221 cgaaatgcct ttaggaacgg cttctatgga aaagcggggc gtcgctttag aagtacctga
     5281 gtggattagt gatcgctgta cgatgtgtaa tgaatgtgcc tttgtttgtc ctcatgctgc
     5341 catccgtcca tttttagcag atgaagagga aatggaagaa gcgccagaag gatttatcgt
     5401 gagagaaatg cgaggcgcag acggcgtaaa ataccgtatc caagtttcag tggaagactg
     5461 tacaggttgt ggtctgtgtg tagaggcttg tcccgcaaaa gaaaaagctt tagtgatgaa
     5521 gccttatgaa gaacagaaag aacaagctgt gaattgggct tttgcaatga cattaagaca
     5581 aaaagcaaat ccagtcaaag gaaagaatac tgtcttgtct acacaattca atcaaccgct
     5641 tcttgaattc tcgggcgctt gctcaggctg tggcgaaaca ccttatgtaa aattattgac
     5701 acaaatgttc ggtgatcgaa tgatgatcgc gaatgcgaca ggctgttctt ctatttgggg
     5761 cggtgcttct cctgcttctc cttatacgac caatgaatgt ggtcaagggc ctgcatggtc
     5821 aaattcttta ttggaagata atgctgaatt tggttatggt atgcttctag cggcacagac
     5881 tcgtcgcgag gctttggcaa gttcaatcaa agaagtattg ccaaaagtat cagaagaact
     5941 acgagaattg atggaagact ggttggtaca catgtttgag agcgaaggaa cgcagcaaag
     6001 agcagcaaaa ctgcaagctg cgatcgaaag cgaacgatca attgtccctg aactggagga
     6061 tatatacaag agaaaggatc tattcgtaaa aaacagccaa tggatgattg gtggagacgg
     6121 atgggcttac gacattggtt ttggcgggat cgatcatgta cttgccagcg gagcagacgt
     6181 gaatattctt gttttggata acgaagtcta ttccaatact ggcggtcaaa cctcaaaagc
     6241 aacaccagct tcggcaatcg ctaaattttc ggctagtgga aagtatgctt caaagaaaga
     6301 tctaggaatg atggcgatga catatggaaa tgtttatgtg gcgcaaattg cttcaggtgc
     6361 caatcaaatg cagacgataa aggcttttga agaagcagaa aagtatcctg gaccttcttt
     6421 aatcattgcc tatacgcctt gtatcacaca tggtttgctt ggtggaatga aagaatcgct
     6481 taaagaagca aaagaagctg tttcttctgg ctattggtca ttatatcgat ataaccctgc
     6541 aatggtcgaa aaaaacaaga acccaatgac tttggactac aaaaaacctg atttctctgc
     6601 tatgcctgat ttcatgagga aacaagttcg cttttcctcg ctagagaacg caaatccaaa
     6661 tcttgcaaaa gctttatttg aaaagactat ccaagacgct aaaacacgtt tttacaacta
     6721 tgctgcactg acgggagaat tagaaaaaat caagaaacga cttgaaccag aggaatctat
     6781 cgatgtaccc gttaagacta gaaaagaaaa aacgacggac cttgaagcag aagaaagaag
     6841 agctgccaga gcagaacgac gtaaaaaaat ggaacaagct acaaaagaaa aaaaggaaaa
     6901 ataacagaaa aaatataatg aataattctc tgaagtttaa aaatatttat tgcagagaaa
     6961 aataaaacgc atacttgcaa ttttcctgaa aattatgcgt ttttctcaag aaaaatacga
     7021 gcagatcagc ctctaaaatt aaaacatatg atatactaga gaggaaatct caaaagagaa
     7081 gagaggcttt ttatgtcatt tcaaatcggg gaacttttta atttggacta ttggcagcaa
     7141 atgttttccg gtgacttgtt ctctctgaat tttttggtga acatacttga tattctggtt
     7201 gtctggtatc ttgtatacaa actgatccaa ctgctgcgag gcacaaaagc gattcagctt
     7261 ctgaaaggtg tagctatttt tattgttatc cgaatcgtag cggaaatcat tggtctgcac
     7321 accttgtctt ggctgatgaa ccaagtcatt acgtacgggg tgatagctgc tatcgtgatt
     7381 tttcagccgg aaatccggag aggactggaa catcttggac gcagttcgct tttcaaacaa
     7441 tcaaaaagcg aacagcagga agatgagaag atgatcttgt cttttgacaa agccatccaa
     7501 tatatgtcca agcgaaaaat cggtgcgcta attactatcg aacggcatac tggattagat
     7561 gagtatattg agacaggaat tgcattagat gcagatatca ctggagagct gctgatcaat
     7621 atctttattc ctaatacacc gctgcatgac ggtgcagtta ttgtaaaaga aggaaagatc
     7681 gcagtagcaa gcgcctatct tcctctatca gaaagtatgt tgattccaaa agaattcggg
     7741 actcgacacc gggcagctgt cggtataagc gaagtcagcg acgcgattac catcgttgtc
     7801 tctgaagaaa ctggggatgt cagcattaca ttagataatg aactgatggc tggcttatcc
     7861 cagcaagaat atttggcaat tttgcgtaga gaactgattg ctgaagagaa aacaaaaaac
     7921 aagaaaaacg tgattcaatc tttcctagac ggtatgacta agggagggga tcgcaaatga
     7981 tctcaaaaga gaaacggaca agcattcttt acagttttat tgcattgctg ttcagtattg
     8041 cgctgtactt caatgcaaat ggccagtccg ttccaaatac gctttctgga aatgaagcgt
     8101 ataatcaaac agttactggg gtgccaatca agatttctta tgattcaaag cgctactata
     8161 tccatggata tgaaaatgca gtgactgtca aactttccag tgcaaaccga gttcagttga
     8221 atgcagaggc aaacgaagat acacggatgt tccgcgtcac agcagatatc actaagctgg
     8281 gcgaaggaac acatgaggtt ccgctaaaaa ttcaaaattt gagcagtgct gtcacagcaa
     8341 agatcgaacc atcgactatt acagtaacgg ttgagaaaaa ggtaataaaa gatttcgatg
     8401 tggaaacgtt gattccttca acgttcacac catctggata tgaacttgat gatacttccg
     8461 ttagtcctaa aaaagtctct atcactacag gtgacaaaac attggcagaa atcaaacgag
     8521 tagtagctga tgtggacgct tcgatggtca cagatgatgg gattaatagt gaggtcccaa
     8581 tacaggcgct gaatgcagca ggggaagtat taagtatcgt ctctgatcca gtgcaagtaa
     8641 cagtgagagc tgattcgatc aagccaagca agtcggtacg actttatggg atacagcaag
     8701 gaacaccagc tgccggggta gaaagctatg atttcagttt ttcaaaactg gaagcagaag
     8761 taagcggctc tagcgatttg cttgcatcga tcggtgacag tattgctgta ccgatcaatg
     8821 tctcaggtat caatcatcga acaactcgta aaattgaaat accagtagaa gaggggttaa
     8881 gtatcaagcc cgaatcagtt agtgtggaga ttacacccgt gttgcaaacg tcaagcagta
     8941 gctctgcttc gacgagcagt cggacacaag aaaatacaag tacgagtaga agtatttcat
     9001 ctgctgattc aggaacggaa ccatcgacaa caaccatttc ttccagttcg gaatctggga
     9061 caagcagctc gaccacaagt acagaagaaa catcagaaac aagcgaatca acacaaatga
     9121 gcagaacaaa ttcctcagaa aattaagtta aaggagtaat tttacaacta tgggtaaata
     9181 ttttggaaca gacggcgtac gcggtgaagc caacaaggaa cttacgccag aacttgcatt
     9241 taagctggga cggtgcggag gttatgtcct aagccagcat gaagaagaag gaaaacgccc
     9301 acgcgtgtta gtagggcggg atacaagaat ctcaggtcaa atgttagaag aagcgctgat
     9361 ttcaggactt ttatcggtgg gtatcgaagt gttccagcta ggtgtgatct ctacaccagg
     9421 tgttgcttat ctgacacgtt tgcaaaaagc aagcgcaggg gtcatgattt ctgcttctca
     9481 taatccagca caagataatg gaatcaaatt tttcggtgca gatggtttta aactagtaga
     9541 tgaccaagag ctagaaattg aagcattatt agatgctgaa gtggatgaat tgcctcgtcc
     9601 atcagctgaa ggattgggaa ctgtagaaga atttccagaa ggcttattga aatattctca
     9661 attccttgta caaacgatca acggtgacct ttccggtttg accatttgtg tcgatgcagc
     9721 aaacggagca acttcaacaa gtgttaatcg tttgtttgcc gatcttgaaa cagattttta
     9781 taccatgggc acaaatccag acgggttaaa tatcaatgat ggcgtaggct ctacgcatcc
     9841 agaacgttta gctgagatgg tcgttgaaaa aggagcagat gctggactag ctttcgatgg
     9901 cgatggcgat cgtattattg cggtggatga acttggaaac attgtcgacg gtgataaaat
     9961 catgtttatc tgtgctaaat acttggcaga aaaaaatcga ttgaaaaaag atacgatcgt
    10021 tacgactgtc atgagtaact taggattcca caaagcggtg gaaggaatcg gtttgaaaga
    10081 tgtcgttaca caagttggtg atcgttacgt tgtagaagaa atgcgcaaaa acgattataa
    10141 ctttggcggc gagcagtctg ggcacatgat tttcttagat tttaacacaa ctggagatgg
    10201 gatgctttca ggaattcagt tactgaatat catgaaacag acaggtaaga aattatcaga
    10261 gcttgcagca gaggtaacaa tctatccgca aaaattagtg aatatccgag taagtgataa
    10321 aaacggtgcg atggatgttc cggcaatcaa aaaagtgatt gaagaggcag aagctgaaat
    10381 gggatcagaa ggaagaatcc tagttcgtcc ttccggtaca gaaccattac ttcgagtgat
    10441 ggcagaagcg cctactgatg agaaagttaa ttactatgtc gataaaatcg cggcagtagt
    10501 caaagaagaa atcggactgg attaagagat attttcataa aaaataaaag aacaaggacc
    10561 agtgtcagat gtctgacact ggtccttgtt cttttattat tctactgtca ctgatttcgc
    10621 taaattacgt ggtttgtcaa cgtcgaatcc ccgtaatagc gttgcgtaat atgcaagtaa
    10681 ttgagtcggt acgactgtgg ttagagtagt tagataaggg tggacagttg gtagcaccag
    10741 ctggtcactt tccttttcta atccctctga gacaataact agtgttttag cgccacggct
    10801 ttcgacttct ttcaggtttc ctcgagtatg cgcaccagtc acttcttcac tgatgattcc
    10861 aatcactggt gttccttctt cgatcaaagc aatcgttcca tgtttcaatt cgccagcggc
    10921 aaaaccttct gcctggatat aactgatttc tttcaatttc aaagcagctt caagagaaac
    10981 gttgtagtca gcgccacgtc caatgtaaaa ggcatttctt gtatcgctga gatatacttc
    11041 tgccaactct tctaaataat ctttttgggc gatgaccgat tccatcgcat tagcaactaa
    11101 actcaattca tgagtgatat cgaatacaag agaagtttca aattcttttt tatcaccgat
    11161 agctttggct aataaagtta gtacagctat ttgtgcagta taagctttcg ttgatgctac
    11221 tgcgatttcg ggtccagcat gcaatagcag tgtaaaggat gcttcccgcg aaagagtaga
    11281 accagcaaca tttgtaatcg taagtgaagg gtagccgaga cggttgattt ttaccagaac
    11341 ttggcggcta tctgctgttt cgccactttg actcaagaaa ataaagaatg gtttttttga
    11401 aagaagaggt gtgttatagc caaattcact ggctaaatga acctcaacag ggatctgtgt
    11461 caatttttcc agagtctgtt tacctgctaa ccccgcatgg taactagttc cgcaagcaac
    11521 gatatagatt cgatcgctat cagacaatgt atcgaccagc ttttgatcaa gagagacacg
    11581 attgtcttct gttacgtatt tttgagcgat acgacgcatg ataataggct gttcgtcgat
    11641 ttctttcagc atataaaaag gataggtacc tttctcgata tctgaagaat ctacttgtgc
    11701 ttcgaatgaa gaacgttcta cgatgtcacc tgcaaaattt tgaataataa ctttttctga
    11761 tgtgacggta accatctctt catcttcaat ctcaacgaat tccttcgtat gagcaagcat
    11821 agccattgca tcgctggcaa tcacattgaa tccatcgccg agtccgatta atagtggact
    11881 tttattttta gcgacgtaaa taacatcagg tgctgtccga tcaatcaaag cgaaagcgta
    11941 agaacctttg atgacccgca aagcttttaa aaacgcctct tgcgttgtag tatccttcgc
    12001 gaaagtttca attagatgag cgataatctc agtatcggtc tcaccaataa agtggtcatt
    12061 tgctaaataa gcaattttaa gttcttcaaa gttttcgatt acgccattgt gaacgagcac
    12121 gaagcgtcca ctttgggaag tatgtggatg ggcgttttct tcagaaggtt gcccgtgagt
    12181 tgcccagcgt gtatgaccaa tacctatatt tcctgtagta tcaatattta ttttgttttt
    12241 caaattttga attctgcctt gtgctttgac cagataatca gttgtgccat ctgagacaaa
    12301 gataccagct gaatcataac cacggtattc taatttttct agaccgtcaa tcaatcctgg
    12361 tgttacattt tttaatccaa tcattccaac aattccgcac ataaaaaaca tcctactttc
    12421 tataatgaat tcgtatgaat gcttccagct gcaggaaaat ccttttgttt tatctttgca
    12481 gatatttttt gtggattttc tgtagagtgc agtccctagg gccatccgcc gaatctttcg
    12541 ataaaccctc tcctcgtcat ctgtttacac agacctggcg ctatcctaaa aggtataact
    12601 atcctcctct tttgtttttt atctggaaac atttaaaatc ataacctttt gcgaatgttc
    12661 ttgtcaacaa aaaaagaata aaaaataaat tggtatatat caaatgcttt gtcaaatgct
    12721 ctttttatgt caaaaaataa tgggttttta tctatcatct ttttcaggag ttcggtacaa
    12781 tagaaagtaa tcggatagag gaggagtaaa aatggattca aaaattgaga tcatgacact
    12841 gggaatgtta aaaaaacaat tgtccaaatt cgaagcatca gctggtgtat cagatgatac
    12901 gaaaatattt ttagatactg gctgggatag catccaagaa atcgcacctg atgccttgga
    12961 agttgctgaa gctcgtgagt ttacggtaga agatgaattg acaaaagagt cgttttccgg
    13021 gtatgcgcgg gaagaaaaag cagagcggtt cgacacttct gagcagtcag aaactgtgat
    13081 cgtcataaaa aacttgtatt agaaaggaga aagaatgaat aagagacgtt gggcagcagt
    13141 tctgattgct gcaggattat ttgtggtttc gttagtttca gctagtttga ctaagcctca
    13201 ggaaaacgat caaattgagg gattgaacag ttggttatat ggggatgaag aactagcttc
    13261 aaatgtgttg gaagagggag acagtacgca aaaaatcgta aaattatcag tagatggaac
    13321 gattgctgcg ggaggggctt caggtttatt tgcttctgaa ggttacaatc ataaaaattt
    13381 tatggaacaa ctgaaggcga ttgaagaaga tccaacagtc aaaggtatct ttttagaagt
    13441 aaattctcct ggtggcggtg tctacgaaag tgcggaaatc gctaagaaat tggatacgat
    13501 tcgtaaagaa catgatatcc caatgtatgt cagcatgaaa aacatggcag ctagcggtgg
    13561 ctactatatt tctgctcaag cagataaaat ctttgcaaca gaagaaacag tgaccggctc
    13621 aattggtgtg atcatgtcag gattgaatta ttcaggactt ctagaaaaac tgggtgtgga
    13681 agatacaaca gttaaaagcg gagcgttgaa agatatgggt tcaggaacgc gaccagagac
    13741 aaaagaagaa gaagcggtgc ttcaagcata tatcgacagc gcatatagtc gttttgtagc
    13801 aatcgtcagc gagggacgga ataaatctga agaagaagtc aaaaaaattg ctgatggaag
    13861 gatttatgat ggtgtccaag caaaagaagt aggtttagtc gatgaattgg gatttcctga
    13921 agatgcttta aaagctatgc gaaaggaaca acagcttgaa gatgcggaac tagtagaata
    13981 ttcttccacg ccatctgggt ttgctagtac atggttagga agtaaactag cagaattcca
    14041 gggattgaag ccttctgaga ctagtcagat cttatccgtt cttgagaatc tagggacagc
    14101 ggaatcacca aaagcaatgt attattacgg aggtgagtaa gcatgtcaga gctttcaaat
    14161 gaaaataagg aacaagaaaa agataagcaa cagcagcctg ctgtaggacc tgactccaac
    14221 ttttcccaaa aggtactgag ctcttttgaa agagagcctt tgacgcccga agaaatcaaa
    14281 aagaaacaac gtaaatggaa aaagtatacg gacacaagaa atgaaccgcc aaaagcaaat
    14341 gattttccta agtatttcta tgcaggtttt tttatccgaa tgtttgcttt cttggtcgat
    14401 cttttgtgta tcggagctat tacgcgttta acacttggta ttgccaaaaa tctaggttgg
    14461 cttaattttt cagattctta tctgagtatc tacggattca cagccttact tatttatcta
    14521 gcgtatttta ttttattgac gaaattcaat catggtcaaa cgattgggaa aatgatcttt
    14581 ggtattcgag tcatcagctt tgatgaagaa gaacttagct ggtcaaccgt tttagttaga
    14641 gaaggtgctt gccggttcat tctaaaattt cctttgctga tgttagggta tcttccagca
    14701 atctttaatc agaaaaagca acatctaggc gattttttca ctaatacaag tgttgttacg
    14761 atcaatctga tcaaagcatt caaccaggaa gtcaacagct gatgagcaat aaaaagcagt
    14821 aacagttgaa agcaccttct aaaagtcaga tctaaaatct agcttttaga gggtgcttca
    14881 gttattttta attgtcatgc tttttttgtt ttgctactat tgaacatgag ctgaacatga
    14941 gcaagaggag gacactgcgt gaagccgatt gattttccag aaaaatatga gaatttaatg
    15001 cgagtagcac aacaagcact agcaaaccag caatatcagc aggcaaaaga attattttaa
    15061 cgagcttacg agttgaaaga aagctttgag gctaatagcc tgcttgtttt ttgtctttac
    15121 gagttggacg agaaaaaaga agcattgaag caagcactct tgcatgaaaa acagtatctt
    15181 caaaatgagg agtttgctga attttatttt gacttgttaa tcagtacaca agatttttta
    15241 tatgctagaa aattgatagc aagtacagat ttttatgaag ctttcgagca gcgggtcatt
    15301 gaaaaaatcc aatttgcaga agaattatcc ggacaaatgg agaggcagaa gatcaaggca
    15361 ctccacaata aatcggagga acttccttcg ttggaacctg cacgtcagct tgcgtctatt
    15421 gatgagatcg aacagcttcc ttatcatgag tttatccaaa cagcgagtaa acttattgta
    15481 tcgccagagg tccatatttt agcaagggca aaattactgg agacacttag gcaattgaac
    15541 gaatgccatc ctgttttcta tttgacgatt gaagaaaagt tagtcaaagt cataccaaaa
    15601 gatctgccaa agccgcagca gcaggcaagt taccagcaat tatgtgtatt ttcagatcgt
    15661 tacggtaatg aagatgcgct gcttagttcc gtgttaaatg aagaattcac gttgcaatct
    15721 gcaattgttt atcctgtcta tgatacatac atcgaagacc cgaagcgctg gtttcaactg
    15781 actgtagagg cgtgtacagg aaaaacgatg tatgaaggta cagaagatga aaaacaagat
    15841 tttttaaaaa aaagagaaaa aattttgcag caaatgatct tcttccatta gattttatca
    15901 gtgaaaaaga taaaaatgtt atgaaaaaca aggattctat ccattcaata tgtttacaat
    15961 ttgaaagggt tagtgtacta tttaaaggta tgcatgaaac gtttttgatg tataaaatga
    16021 atttctaaac tcggagggaa ttaaatgact gcaacatttg aaaaaaaagg cacgaatgat
    16081 ggtgttttaa aatttacgat tgcgcaagac gaaatccaaa aaggattaac aactgcgttt
    16141 aataaagtga aaaaaagctt gaacgtacct ggattccgta aagggaaagt ttctcgtcaa
    16201 gtattcaacc gtatgtacgg tgaagaagct ttatatgaag atgcattgaa tgctgtatta
    16261 ccagaagctt atgaagctgc tgtcaaagaa gctggcatcg atcctgtttc tcaaccaaaa
    16321 atcgatgttg aaagcatgga aaaaggtcaa gactgggtaa tcactgctga agtaacagtg
    16381 aaaccagaag taaaattagg acaatataaa gatctagttg ttgaaaaaca agaccgtgaa
    16441 gtaacagatg ctgacgtgga tgcacgtatc aaacgtgaac aagaagcaca agctgaatta
    16501 gttatcaaag aagatgctgc agctgaaaat ggcgatacag tagtaatcga cttcgaaggc
    16561 tttgtagatg gcgaagcgtt tgatggcgga aaaggcgaaa attactcact tgaattaggt
    16621 tcaggctcat tcattccagg ttttgaagat caattagttg gtcataaatc aggtgaaagt
    16681 ctagatgtaa atgtaacttt cccagaagat tatcaagcag aagatcttgc tggaaaagaa
    16741 gcagtcttca aagtgacaat ccatgaagta aaagcaaaag aagtgcctga attagatgac
    16801 gaattcgcaa aagatgttga tgacgaagta tcaacattag acgaattgaa agaaaaatat
    16861 cgcaaagaat tagtagaaac aaaagaaaaa gcagcggacg atgcaaaaga tgaagcagcg
    16921 atccgtatgg ctgttgaaaa cgcagaaatc gttgaattgc cacacgtgat ggttcatgat
    16981 gaagtacatc gttcaatgga cgaattcttg aacaacatgc aacgtcaagg tatctctcct
    17041 gaaatgtact atcaattaac tggttctaca gaagaagact tgcataaaca attcgaaggt
    17101 gaagcagaaa tgcgcacaag aacaaatctt gtgatcgaag caattgcagc tgctgaaaat
    17161 cttcaagcaa cagaagaaga aatcaatgca gaaatccaag aattatcaga aacatacaac
    17221 atgccaatcg aacaaatcaa acgcgtgttg acagaagata tgctggaaca tgatgtgaca
    17281 atgaaaaaag caatcgaatt gatcacaggt acagcgacag aagcgaaaga atagaaatca
    17341 agtaataaat catttctgaa gaagctactt ctagaaaatt gtttattcga aataagggac
    17401 tgtgacacga ttttgtcaca gttccttatt ttttgttaag agtaaaaaat gcaaaggtat
    17461 aggtcagttg atcctttgtt aaacactttt tctttttact aaactatgtt atgatagtaa
    17521 aagactgtaa agtctttgat agtttcttgc atactttttg caagttaagg ggtgaagacc
    17581 atgtatgaca atccaagtag caatgagacc gtacgctgtt ctttttgcgg gaaaacccaa
    17641 gaagaagtga aaaagatcgt agctggtcca ggggtctaca tctgtaatga atgtatcgac
    17701 ctatgtaaag aaatcattga tgaagaattt tatgaagaag ctgtacgcga attcactgaa
    17761 gtccctaaac cactagaaat tcttgaagta ttgaataact acgtgattgg acaagatcgc
    17821 gcaaaacgct cgttagctgt tgccgtatac aatcactata aacgtgtcaa tcagcaagca
    17881 caagaaaaca cagatgatgt tgaactgcaa aaaagcaata tctgcttgat cggacctaca
    17941 gggtctggta aaacattttt agcacaaaca ttagcaaaaa cattgaatgt accttttgca
    18001 atcgcagatg cgacaagttt gacagaagct ggttatgtgg gggaagatgt agaaaacatt
    18061 ttgcttaaat tgcttcaatc tgctgattac aatgtggaac gtgcagaaaa agggatcatc
    18121 tatattgatg agatcgataa aattgcgcgt aaaagcgaaa atgtatctat tactcgtgac
    18181 gtttccggcg aaggtgtcca acaagccttg ctgaaaattc tagaaggaac tgttgcaagc
    18241 gtgccacctc agggaggacg taaacatcca catcaagaat ttattcagat tgatacgacg
    18301 aacatattgt tcatcgtagg tggtgcattt gatggcatcg aaacgattgt gaagaatcgt
    18361 atgggcgaaa agacgattgg atttggtaca caaaaccaaa aactttctga agacgaaagt
    18421 gtcatgcagc atatcattcc tgaagattta ctaaaattcg gattgatccc tgagtttatc
    18481 ggacgattac ctgttatggc agctttagag aaattgacaa cagatgatct ggtacggatc
    18541 ttgactgagc cgaaaaatgc gctagtcaaa caatatcaaa aacttctttc tctagataat
    18601 accgaacttg aattcgaacc tgaagcgttg agagccattg cgaaaaaagc aatcgaacgc
    18661 aataccggtg ctcgaggact tcgttccatc attgaagaaa tcatgatgga tgtgatgttt
    18721 gatatccctt caaatgaaaa catcgaaaaa gtgattatca caaaagaagc agctgaattg
    18781 tctggtgagc caacagtaat ctataaagat tcagagaaaa aagcgggttg atgaaaagag
    18841 gctgccgtaa aagtcatttt ggcttttgcg acagccgctt gctttattca tcaccttgtt
    18901 aaggagagag accatgaaag tccatcaagc tgaaattatc ataagtgcag tggcaccaaa
    18961 acaatatcca gatacacaat tgccagaaat agctctggca ggacgatcaa atgtagggaa
    19021 atcttctttt atcaacacat tgattgatcg aaagaattta gcccgtactt ccggtaagcc
    19081 aggaaagacc cagacattga atttttattt gatcgagaac gcattacatt ttgtcgatgt
    19141 tccgggatat gggtatgcaa aagtatccaa aactgagcga gcaaaatggg gaaagatgat
    19201 cgagacatat ttaacgacta gagaccagct acgtgcggtc gtttctttag tagatttgcg
    19261 tcataagcct tctcaagaag atatccagat gtatcaattt ttgaagtatt atgatatccc
    19321 tgtaattgtt gttgccacta aggcagataa gatccctcgt ggtaaatgga acaagcatga
    19381 gagcgaaatc aaaaaagcgt tggattttga tcgcaatgac acgtttattc tcttttcttc
    19441 cgttacaaaa cagggaaaag aagaagcgtg ggcagcaatc gaacaagcaa ttgaatcata
    19501 aaaagaataa aaaagactta ccagtcaaac tctttcataa aagtttggct ggtaagtctt
    19561 tttttgagct taacttactt ttgtttcttg tcttttttct tttcattttg tggctgtggt
    19621 tttagaaatc gatcgatctc ttcttttggt gcaactgttt tttccggatc gacagcaaat
    19681 gattcgaaaa gtttatctaa attatctgta cgttcaaatt tcaaccccat tgtaaacacc
    19741 tctttgtttc tttttactta gtctatcatg taatcgtcca aattggtttc taagacataa
    19801 agttttagtt tttctttatc gttggttata attaaattat aaaaagattt taaagaggtg
    19861 agaaaatgat tggcgaagat aaattattga tgactatcca gctcacagac ttaaatgaag
    19921 aagaaaaaaa acaggaaata agcgattggg ctattttgac acgtctgttg atccagccaa
    19981 catttatccg ctctttcaca caacaagcgg atccgtatga tcttcgaaag cttcaagaaa
    20041 aaataatgct agcatcggta agagacgaaa gttggttagt tgtaggaaat gacgagaacg
    20101 aatgtagttt tcgcttggaa gataatcaat tattgataaa aaatgtattg tctatctctg
    20161 ttttcaaaga aaagcaattt ttgattcgag acttcattca gaaaaaaatg attgctcatg
    20221 gggtgttcgc ctatatgcga gcttatagcg agtttatcta tcacaataca aaacagatct
    20281 ctcaacggct attgtttgag aaaaaggatg aaatcgaaca tttacctaaa atgaaacaac
    20341 aaaacgggga agtagtagtc gattgcaacc aatttccagg atacgatctt ttttaccaag
    20401 gtttatgttt cacttcgtgt tgggaaatgt attatagtcg ctattatcat caaatcatcc
    20461 ctaaaccgat ttttctcgac atccaacaag tagaaaaagt gaaggaatta gaaaacgaag
    20521 tcatatgtat ccagctttat cgagacccct ttaactggca aaagccaaac aatcaaatgt
    20581 ttcaatccta ttttagggac cagcttgggt ttgatcatct tgcttgggac aatggtgtcg
    20641 gtttattgaa accgccattc gttgaataca tttacactga ccatacgatc caatctgtcc
    20701 agtatcagaa tgcacaaatg caacctgttc ctaaaaagaa tgcttcattt tttgtaacaa
    20761 aaagttatga tattcaacaa ggagattata aagaaaggcg tgttcgcgga acactaaatg
    20821 ctcaggcgta ttttccttgg gttgacgaga atcgctcgcg catgatgtgc tataaagtta
    20881 tcgatccgac tgttgctttg gataatggaa tcgaagctta ttgttattat attagggaat
    20941 atctagaagt agaggtaaca gatgaaaaat atcaagagta tcttttatcc ttaagaatct
    21001 atgttccttc agaatccctg tcagaattac cgttaaagga aatcaaacat agactctctg
    21061 atgttacctt caaacgaata aagaaaaagc ggcgaagtat ccaattcgat gtcaagaaag
    21121 gtgagcagca tctgcgtgtg caatttttag attaccgaga attagaacaa ttgattactt
    21181 tacaaaaaat atgaggtgaa gaaaatggat aaacaactct attatcgaat aatggttccg
    21241 gcatcattat tatccgtctg tctctttttt gttcctaatt ttcctattcg atcagtcatc
    21301 ggttttttgg tcatgtttgg cgggatggga ctttattatt atcttcttca taaggaaaga
    21361 aaacctgctt ctgtttatat gaaaaaaaca agaagcgaaa ataaaaaacg ataaaacggc
    21421 ttgatgatag ac
//
LOCUS       DACDHL010000043        21360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDHL010000043 DACDHL010000000
VERSION     DACDHL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.043"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            95..283
                     /locus_tag="IV228_001643"
     CDS             95..283
                     /locus_tag="IV228_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717539.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
     gene            complement(927..1880)
                     /locus_tag="IV228_001644"
     CDS             complement(927..1880)
                     /locus_tag="IV228_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8717540.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1903..2658)
                     /locus_tag="IV228_001645"
     CDS             complement(1903..2658)
                     /locus_tag="IV228_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8717541.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2655..3614)
                     /locus_tag="IV228_001646"
     CDS             complement(2655..3614)
                     /locus_tag="IV228_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8717542.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3607..4557)
                     /locus_tag="IV228_001647"
     CDS             complement(3607..4557)
                     /locus_tag="IV228_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8717543.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4707..5159
                     /locus_tag="IV228_001648"
     CDS             4707..5159
                     /locus_tag="IV228_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8717544.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5248..7515)
                     /gene="gshAB"
                     /locus_tag="IV228_001649"
     CDS             complement(5248..7515)
                     /gene="gshAB"
                     /locus_tag="IV228_001649"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8717545.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7702..8700)
                     /gene="ftsY"
                     /locus_tag="IV228_001650"
     CDS             complement(7702..8700)
                     /gene="ftsY"
                     /locus_tag="IV228_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8717546.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8711..9520)
                     /locus_tag="IV228_001651"
     CDS             complement(8711..9520)
                     /locus_tag="IV228_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8717547.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9529..13110)
                     /gene="smc"
                     /locus_tag="IV228_001652"
     CDS             complement(9529..13110)
                     /gene="smc"
                     /locus_tag="IV228_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8717548.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13130..13816)
                     /locus_tag="IV228_001653"
     CDS             complement(13130..13816)
                     /locus_tag="IV228_001653"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8717549.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14164..15945)
                     /locus_tag="IV228_001654"
     CDS             complement(14164..15945)
                     /locus_tag="IV228_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8717550.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15971..16885)
                     /locus_tag="IV228_001655"
     CDS             complement(15971..16885)
                     /locus_tag="IV228_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717551.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16912..17874)
                     /locus_tag="IV228_001656"
     CDS             complement(16912..17874)
                     /locus_tag="IV228_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717552.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17874..18818)
                     /locus_tag="IV228_001657"
     CDS             complement(17874..18818)
                     /locus_tag="IV228_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717553.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18819..19823)
                     /locus_tag="IV228_001658"
     CDS             complement(18819..19823)
                     /locus_tag="IV228_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717554.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20091..20333)
                     /gene="acpP"
                     /locus_tag="IV228_001659"
     CDS             complement(20091..20333)
                     /gene="acpP"
                     /locus_tag="IV228_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8717555.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20455..>21360)
                     /gene="plsX"
                     /locus_tag="IV228_001660"
     CDS             complement(20455..>21360)
                     /gene="plsX"
                     /locus_tag="IV228_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8717556.1"
                     /translation="FLLYGKEAEIKKYLTNEKNITIIHTDEKINSDDEPVKAIRRKKT
                     ASMVLAAQAVKNGEADAVFSAGNTGALLAAGLFIVGRIKNIERPGLMSTLPVVGKEGA
                     GFDMLDLGANAENKPEHLLQYGILGSFYARKVRGIGRPRVALLNNGTEATKGSEVTKK
                     AYELLQNETSLNFIGNVEARELLNGVADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSA
                     ILNEGIKGKMGAMLLKDGLRSLKAEMDYSKHGGAVLFGLKAPVIKTHGATGPDAVRYT
                     IRQIHTMLETDVVGQLVEQFEKSEE"
BASE COUNT         5921 a         4584 c         3775 g         7080 t
ORIGIN      
        1 aaaaaacatc tattttatcg aagaaacaca aaatatcgag ggttcttata ttgaagtaaa
       61 aacactcttt gtcaatgaag ataaaaccaa agcattggat atttataaaa aattagcttc
      121 aaaaaaaacg aatagttttg gattgatatt aagcgagtat aaaatcaaag cagaagagag
      181 ttatttctat caattactaa aacggtggag taagcttccc gccgattttt atcgaaaaat
      241 gcagatcatc aactatcaac cgcttgctga gacacatgcc taaatagagg ttgtgaaatc
      301 agtgctttga gctaataccg aaggtcagct gattgaacat tgtttagaag agattgtgac
      361 aactgcgatt tgctttgagt aataagaacg ctggacagaa aatagcttct catattttct
      421 agtctagtgg gcttattgcc gagaagcagc agatggaaca tcgtttagaa gagattgtga
      481 caactgcgat ttgttttgag taataagaac gctggacaga aaatagcttc tcatattttc
      541 tagtctagtg gacttattgc cgagaagcag cagatggaac atcgtttaaa gaagagatcg
      601 gtacaaaata aaaatatcta aatttaagtt ttatctaagt ttcgctttgt acctttgatt
      661 caaatcaaag gaggcgatct tgatgctgaa aaaatggatc acactatttt ttgccactcc
      721 ccattctaga atcaacccgt atgatgtaga taatgaattc acgttgaaaa aagatttctc
      781 aaataaataa aaaccatccg taaaatcatc aatacgattt tacggatggt ttttgataac
      841 ttctttgagg ataggctctt gctatttaag aatgtaagaa acattattgt tacatagtat
      901 gagagtttaa tcctctgtta tttatgctat ttaaatgctt gattcacatc ttcgatcatc
      961 agtttcattg attctaaacc gcccccgctt aaataccaga catctggttc aagggagatg
     1021 atctgctgat ttttaccagc attcgtttgt gctaccagtt cattggcaga aatatcatct
     1081 ttgctgtcgt ctccaccaat agcttttgtt ctgtctacta caaataagat atccggattt
     1141 ttttccagca catattcata agaaacactt tgaccgtgag tggatgcttc gatttgatca
     1201 tctgcttgtt caaagccaaa taaatcatgg atgaaaccga atctagaacc agatccgtag
     1261 gctgataatt gcccttcatt cacaagtgtg actagtgctt ttttgtcagt agcttctgct
     1321 ttctttttcg tctgatcgat tgcactagat aattcctcta attgagtttt agcttcttct
     1381 tgcttaccaa agatctcccc taaagcagtg acattttgct ggaaagattc ccatggattt
     1441 ttcgcatcta aagaaaggaa aatggtcgga gcaatttgac ttagatcttt ttgaaaatct
     1501 ctttgtcggc cagaaatgat gattagatct ggctgaagct gattaatttt ttctaagtct
     1561 ggttctttta ttccacctgc tgattctact tttttatagg atgaaagata ctcaggaagg
     1621 ctgcttgtag ctgctccaac taccttatct ccaacaccaa gagcatccat cgtatctaaa
     1681 gaaccattgt cgaatacgac gactttttct ggattatttg gcacctcgat ttggtctcca
     1741 ttactatctg ttatcgtcaa tgtagtttca gtttgtgtgc tgcttttttt tgtgtctgcc
     1801 tgactggtgt tgttgctatt tccacatgct gctaaaccaa atgatgctag aatcaagata
     1861 cctactacta attttttcat ctcttctctc ctaatgttag ttttagaaat aaagacagaa
     1921 tctgcggcct tcaatctcac aaatccgtat gtccatttcg tataattcat ccaatacatc
     1981 tttttttatc atttcttggg tcgttccttg cgtaaataac tgtccatctt tcattgcaac
     2041 aatttcgtct gcataacttg cagcaaagtt gatatcatga agaacaataa caattgtttt
     2101 cccgatttct tgtactaaat tttttaaaat ctgcatgatt tgaaccgcaa aattcatatc
     2161 tagattgttc aaaggttcat ccagtaaaat ataatccgtg tcttgcgcta gcaccattgc
     2221 aatatacgca cgttggagtt gaccgcctga aagagtgtca atcgattgat ccgctatttc
     2281 ttctaaccca aggtgttcca gtgattcctg aacgatttcc tcgtcttttg tcgtcaattt
     2341 tcctttcgta taaggaaatc gtccaaattc tactaattct ttcacggtga tttggacatt
     2401 tatttcattc gattgtcgta ggatcgataa ttttttggct aattcattgg atttccaagc
     2461 ctttacttcg ttatggtcta aatagatttc acctgtatct tttggtatca aacgactgat
     2521 catcgacaac accgttgact ttccagcacc atttggaccg ataaaagcag tcaacttctt
     2581 ttcaggaatc gttaactgta catctttaac cactgatttt ccatggtaca gtttattgac
     2641 cttttgtaaa tcaatcatag gatactcttc ctttcgcgaa tcaattttgt gacaaaataa
     2701 attcctccga caaattcaac gacgacactc actgttgtat tccagccgaa aatctgctct
     2761 actgcgagct gtcctcctgt cagaaacagc atagctacca gaaagcttcc gataaatagc
     2821 tgaccatgac gataagtaga aaagacacga taactgatat ttgcgacaat aaaccctaaa
     2881 aaagtaactg gacccacaag agccgttgat gtccctgtca aaagactgac acaacataaa
     2941 atcaaaaatt caaaacgatc tgtgtctatc ccgaggttta ctgcttgttc atttccgaga
     3001 tggaaaacgt ctagcacatg tcgatatcga aataacaggt accagcagcc gataatgatc
     3061 aaagaagcag gcaaaagata ctgactctgg atattgccaa agcttgcaaa taatctgcct
     3121 tgaagcaaat catattcatt cggatccatg atgacttgta aaaaggtact gacactacca
     3181 aaaagcgtac ctaaaatcat ccctgacatc agtaataaaa aaagattcgc tctcattttt
     3241 ttcaaaagaa agaaaaataa caatagactc agcccgacca tcagcccaac gttcaataag
     3301 aaaaaaactg tacctgtctg ctgaagcatc gtcactcctc cgataaagaa gaaaaggacc
     3361 gtttggacaa ggacatataa cgactcaagt cccaatactg aaggagtcaa aaactggtta
     3421 tgcgtaattg tctgaaagct gattgtggca ctgctgacag ctagcgagac aaggataaat
     3481 gcaaaaattt tcttactcct caatgcacat gcaaaatgcc agtttccata tgtatggatc
     3541 gtcaagtaca ttcctgtaac aaataaaaca agaacaagca taccaataaa aatagatggc
     3601 ttttttttaa gcatatcgtc gtccccttat cagaagataa aggaagatag ccccaccgat
     3661 gattccaacg atcacactga ctggtatttc ataaggaaaa atcaacacgc gagcgacaag
     3721 atcgcaaaat actaagaaca aactacctgc taaagcagtt acgccaattg ttttgtgcac
     3781 ttgatctcca tatattctag agacaagatt cggaatgatc actccgataa aaggaatgct
     3841 tcctaccatg atcaacatga ctgcactagc caatgatact aagccaatgc ctaaaaactg
     3901 catcttctgg taatctattc ccagatttac tgcaaacgat tcgcccagac caatgatcgt
     3961 aaatcggtaa gcaaatagcc aaaccaagag caacaatgga acaacagcat acaataactc
     4021 ataatttcct cgtatcacag tcgcaaaatt cccttgaagc catgaagtca tattttgtac
     4081 caactggaat tgataagcaa aaaaagtagc aactgaacca atgatattcc ctaacatgac
     4141 tccaatcaac ggaagcatca attgattttt ctggaggaac aatcgcgtaa gggataaaaa
     4201 aagcaatgtt cctgcaaaag caaagcaaaa tgctgtcaag gatcggacaa tcaaggatgc
     4261 accaggtagg aaaatcatag ccactaaaat acctaaacgc gcactatcca tcgtgcccgc
     4321 agtcgttggt gaaacaaatt tattttgggt caaatgttgc atgatcaacc cacaaatact
     4381 actggtcgct cccacaagga tcaaacaaat cgttcgtggc aaacgagtca ctagcaagat
     4441 ttgtaagctt tgcccatcta actggagcag ttctgacaat tgtacattcg atactccgat
     4501 gaacaatgaa atactactta aaataactaa taatgctact agtcgtccgt agcgcatcca
     4561 atccaccttt ctaattgaga atgattttca ttcttatttt aagtgaatcg ttgaacaaaa
     4621 gcaaccgctt tttcatatgt tttaggtatt attgggaata tgctataatc gtaagaaaga
     4681 caaagagaag aaggagggta ttttttatgg cacaagatga tgtaaaaagc catttggata
     4741 agggacttta tggtacacct ttagtgaacc ctgaagaaca gcataaatat ttagggacat
     4801 tcagggaacg ctgctatctt agtatgactg ttgctgaaat gatccaacca aaaaacaaag
     4861 aaaacttttt aaaagaactc aaaaaacatc cagatgcttc catactcttg aatggggctc
     4921 tacccctttc tgtccaaaac caatatatcc agctagcaac gaaaaacaat agccgtttca
     4981 ctgtagtcaa cgattacgtc actgcaaatc ctgaggaatt cggattgctc ttaacagcaa
     5041 aagaagcagt aaatgaacct gtgattgata tcgaaaaaaa atacccagaa gaaaactctt
     5101 cgccagctga aaaagaaaaa ccaaaaaaga aaaaaagctt ctgggatcgt ttattttaaa
     5161 acgaaaaaac acataaattt ttacatgcaa aaaaagcttt tgatcagatt gacatcaaat
     5221 aggacaatct gtcaaaagct ttttctttta aaatacttca ggatataaca atttcaatac
     5281 attcatcgtc aatcttctgc cttttcctgc aaaaggataa acatgcatat gcatggctgg
     5341 attgaaattt gcttcgatga tcccatatgc ttttttatca gttgtaggat cgatttcttt
     5401 atctggtata atcaaatcga taccactaat cttagctcct agtgcttcta ccgcagaaac
     5461 ggcgattttt ttgtaagctt cagaaaattc atcagtcata tcgatcgaat caccacctgt
     5521 actgatattt gagttctccc ttagataaac aatttgattg gcttggggaa ctgattcgat
     5581 cgtcaaccct tgttctttca acatcaattg ttctaattca cctaattgga tcaattctaa
     5641 aggtgcacga tgattcgttc cacgcaaagg gtcactattt ttttcttcaa caagttcttt
     5701 cactgttcgg atactgtcac caataacatt agcagggact cgcaacatga tggcttggac
     5761 ttctccgtcg atcacaaaga aacgatattc cgttcccggc ataaattctt ctactaaaac
     5821 cgaactgtct tcacgaaaag caatggctaa acctgcttga taatcttcca gactagctcc
     5881 ttccttgaaa atcgtgatac ctaacccgta attggttgat ttaggtttga tcacaaatgc
     5941 ctgctcggca aatctcggat aagcttttac cgcttcttcc atggatgaaa attctgcgcc
     6001 tcctggtacc cggaacccag cttcttttaa gacttttttc gttactgttt tgttttccat
     6061 gataagtggg acgatatagc tgtccttact tgtcatgttt gcatttttca catattctac
     6121 atggtcttgg aattgcagac gcaaaaattg atccgactca tctaatactt tgacttggac
     6181 acctttttgg atggcatcga acataaatat ctgagtagaa agttccattt cgcgaaaacc
     6241 ggccaactga tagggacgtt catggctttc tttataatat tgatgtccca atgccacagc
     6301 aacttcttgc tgactcgata gctgtgcttc ttgatagagt ttcgctgcta acgtttgttc
     6361 aggatgatcc atttgagtca atgcttcctc tactaaataa agagaatcca accaatccaa
     6421 ttcaaccagc atttgcttca ttccttcaag aatttctcta ccttcttgat gaaattctgt
     6481 ttgatctgat ggttgttcta gtgctacttt gttatttcga gtcgtacctt ctgctaccca
     6541 ttggtcacag tcttcttttt cttctgtcca taacatgtac atcaggaata attgtaagaa
     6601 ccgaacttgc tctggattga tacccaatct tgcataagga ttcaaatcga tattccgcaa
     6661 ttcaatataa cgaacacctg ttgttgctaa atctgcgact ttctttccgc ctcggaaacg
     6721 aagcggtgta taaaattctt tctcctccga aagtttccct tcttcgatca ttcgctcgat
     6781 atctgctaaa tactgtttca tagaagcata ggaaactttc acatttggat gattcgtata
     6841 gcccagcgca ctgttgcgaa tactgcgaac aggttcttgc ggatgtgatt catctaaaaa
     6901 gtagttcttc tcactcattg gtgaagcacc gaataaataa gtgatcatcc agcgatagcg
     6961 cataaagttt ctcgctgttt tcaaataaag ttctgttttg aattcggaaa aatcttgaaa
     7021 ttcttcctga tggctgaaca atgttcttag caactcatcg ccaaattcaa aattgaaatg
     7081 aataccgctt accatctgtt ttcgcttccc gtattttttt gctaaataac gtcgatacaa
     7141 gacatcttcg aaatttttta attttgcaat aatgatctct tcgtcttttt ccggtaaggc
     7201 aggtggcata cttaatggcc agatcatctc ttctttcggt atagaacgag cagtcacatc
     7261 ataaacagca gcgagatact gaaataattc gggaatagaa tcggtaacag gggtgatcat
     7321 ctctgtttgt gtttcgctaa aatctgtttg gatatagggg tgataggatc gatcgccaaa
     7381 gattgctgga tgatcggttt ttgctagatt tcctgcaaga tcaacccgct gtccttcccg
     7441 ttctatacca aatcgagctt ggtcgataaa agggcgcgca ttgacatgca ataataattg
     7501 cttaaaattc atcattaagt gacctccaat tcatatgaat ctaattataa tcaaaaagtc
     7561 taagaaaaag aacgaaattg tttgagaacc gcttacaaat aagtataaaa atgaaggcat
     7621 caaaaacaca gctatgttaa aaacaatcat tcaaacaagg agctgcaact tttgtcacag
     7681 ctcctgcttt ttagacatgg gttattcttc ttttagtaat cctttgaaca atccaacgac
     7741 aaaatcattt ggatcaaacg gctccaagtc atcgattcct tcaccaagac cgaccaattt
     7801 cactggtaaa tgcaattcat tacggatagc aagtacgata ccgccctttg ctgtaccatc
     7861 taattttgtc aaaacaagac cggtcacatc tgttgtttct ttgaattgtt ttgcttgaac
     7921 catcgcgttt tgaccagtgg ttgcatctaa tacaagaagt gtttcttggg gtgcttctgg
     7981 taattcccgc tggattactc gttttatttt ttctaattcg ttcatcaagt tcactttatt
     8041 ttgcagtcgg cctgcagtat caactagtaa aacatctgcc tgctctgtct ttgctctttc
     8101 caaagcatcg aatacgacag ctgctggatc gcctcctgca ttgccacgaa caacttcaac
     8161 accagcgcgt tcgccccaga caactaactg gtcgattgct ccagcgcgga aggtatctgc
     8221 tgctgccatc aatacttttt taccttcgtt tttgaactga tgggcaagtt taccaatact
     8281 tgttgttttc cctacaccat taacaccgac aaacaaaata actgttagtc cgtcttcttg
     8341 gatatttagt tcatttactt cattgattcc ttccgcttca tagagatcca caagtttctc
     8401 gatgatcgca ttttggactt ctgccgattt tttcgcattt ctgagtttca cttcttgacg
     8461 taatgcttcg gtgattcgta tcgctgtttc aaaacctaca tctgcaccga taagtgtttc
     8521 ttctaattct tcaaaaaagt cttcatcgac tgttcgaaaa ttggcaaaca actcattcat
     8581 ccgttcacca aatgttttac gcgtcttttc taaacccttg tcgtacttct cctgtacttc
     8641 aacttcttct gttttctctt cgccagtaaa agcacgcttg attttatcaa aaaatcccat
     8701 ttgactcccc ctactctaat acaaattttt ggatcacttt ggctacgcca ttttcttcat
     8761 tgctggctgt cacgaaatct gctgcttctt tgacgaaagg cacagcattc tccatcgcta
     8821 cgcctagtcc agcatattgg atcatcggta aatcattttc ctcatcaccg atcgccatga
     8881 tttcttcttg tttcaaacca agatcttttg ccaataaaga aataccataa gcttttgtaa
     8941 caccttttgg cataaattcc aaaagcatac ttcttgtttt gatgatctca tacatttcat
     9001 ggaattctgc tggtatttct ttgattttag gatctaattc ttcttggtca taagcgatta
     9061 ctgccttatt aagcacaaat tcatctgtaa tatcgcttat atccgctggt tggaattgca
     9121 gcagcttatt caacacattg tacaaggatt gcttccttgg ggcactaggc agctgtaaaa
     9181 ccacattgtc agacaaaaca tctagtggca gatccaattg ctgagccagt ttgtataagc
     9241 gttggatatc tgatacttcc atcactttct tttccatgat ttcaccagta tcattttttt
     9301 gtaccaaacc gccgttgaaa gtgatgctgt agtcaccttc atctaccagt cctaattctt
     9361 gcagataatg cgccatcgca gctaaggggc gtcctgtaca tagaacgatc ttgacccctt
     9421 gctgttttgc ttgtgctaat gcttgtttat tctcatctga aatcagcttt tgttcattta
     9481 ataatgtacc gtccaaatcg attgctacta atttgatcat tttcgttcct acactttcgt
     9541 ttcgttgatt ttcgcctctt cattgatatc ttccatccgt acagaaacga tttttgatac
     9601 accggattcc tgcatcgtca ctccgtacag tacatctgct gcgaccatcg ttcctttgcg
     9661 gtgtgtgact acgataaatt gtgtgtcatc ttggaaatcg ctcaagtaac gaccaaaacg
     9721 tttcacgttg gcttcatcta acgctgcttc tacctcatct aagatacaaa acggaacagg
     9781 acagacacgg ataatggaaa agagcaacgc gatggctgta agtgctcgtt ctcctccaga
     9841 aagcaaactt agactttgca gttttttgcc aggaggttgt acttcaatct caatacctgt
     9901 tttcagcaaa tcagttggtt ccgtcagtac taattcagct ttaccgccgc caaacatatt
     9961 tggaaagaca attttgaatt cttgacggat agcttcaaat acttctttga atcttgtccg
    10021 cacttcatca tccatctcat ccatcgtttc aaacaattgg tttttcgctg ctaacagatc
    10081 atctcgctgt gttgctaaga atgtatgtct ttcactgact tgttcgtatt gttcgattga
    10141 atttaagttt actggtccca gtttttcaat ctgttctttt aaggatgaaa cctttgttct
    10201 actgtcttcg atatcggttg tttcttgata gtcttgggaa gctttctcaa atgtcagctg
    10261 atattctgac tgcagatagg tcaaatgatt atctaaaatc atttcagctc ggtctttttg
    10321 gacttctaat ttggattgtt ccgacagtcg tgctttttgc tcacgattac gctctgccaa
    10381 gactgcttca agcttatcga tctccttttg ccttctatct cgctgctctt tggcttttgc
    10441 caactcggct tttaatgttt ctcgctgagc agataattcg ttaatttgtt tttccaaact
    10501 ttcttctgtg acttcatgat cagaaaagtc tgctgtcaat gtcgccaact gcttttctat
    10561 cgcttcttga cgttcggttg cctctgcttt ttggactcgg gcaccgcgga gctggatctg
    10621 cagatgatta aactgctctt tcaagacagc aagatctgct tgttcttgtg ctttttgact
    10681 ttgtacttgt gctcttctgg cttccatctg gtcgctttct tgacttaacg atttgatttc
    10741 ttcgtcgatt ttttgtcgtt gcgattcaag atctgtttgt ttgtctttca attcgttcgt
    10801 ttgcttttcg taatcttcga taaactgctg cagttcacgt gtctcaaaag ttgagatttg
    10861 cttttctttt tcaaaacgtt caagttcatt tatgatgttt tgtagctgat ttgtcgcttc
    10921 ttgttcttca aaacgtaatt gttcgcctcg tgtacgcaaa acttcttgtt cttcggctaa
    10981 acgagctgtt tcttgttgca gctcctgaac ttttttttct tgtgcttgga gttgtttatc
    11041 tgcttcttca aattcactag tcaactgttt caattcttga ttttgtacaa acaaactgcc
    11101 ttggtttcct cgtttattcg ctccacctgt catggatcct ccggcattca tcacatcacc
    11161 ttctaaggaa accactcgat attgatagcg gatcgtttgc gcgattgcat tggcactagt
    11221 taagtctttg gctaaaagga tcgttcctag caggttttga accacagttt ggatttgatc
    11281 gggataactg acttgttcac tggcaatccc tataaaccct tctacagctg ctgcttgtgt
    11341 caatatatga gccggtaatt gtcttggttt gattgtcgtc agtggaagga atgttgctct
    11401 tcctcctctt tgctgtttca aatacgtgat ggcttgccga gcatcttttt cattttcaac
    11461 gatgacatgc tgggctgccc cacctaaagc tgcttcgata gctaacgtga aatctgcagg
    11521 aacatcgatc agttcagcca ctgctccgac aatacccgaa agctgctgtt tatgctgcaa
    11581 gaccaatcga accccttggt aaaaaccaaa atagttttct tggatttctt gaaggctttt
    11641 cttacgagca cgtagctgct ggacttgatt cattaattga tacattttag gctgttcttt
    11701 tgctaatgct tcttggaact ttttcccgtt ttgttggatc atttctaact tctcttttgt
    11761 ttcagttaaa gatgatttta gctgcgcctg cttttgtgtc aattcttctt tttgtaagat
    11821 caatcggtct actgatgctt ctacttccga ttgcttggct aacgtctgct tgctttttgc
    11881 tgtttcttgt atatattgac gttctagata cttcagttcg tttcctacag ctgcttgttc
    11941 ttgcatcaaa tcaacatatt gatctcttaa ttctgccaat aattctttcg ttgatttttg
    12001 gtatttttct aattcttgct gtgtttttat gagatttgct tcagcttttt ggatctctgt
    12061 ttctttttct gctgctgctt tcatcaagct ttcttgaagc ttttcaaaat gtttaacctt
    12121 tttttgtgct tctgctaatg aagtttgata ttcttgcgag cttttttgtg tatgctttgt
    12181 ccgctcctgc aaaacatctt tttgtccttc tgtctgcttc agtttttccg aaagatctaa
    12241 taatacttgc tgattttttt cgatcagacg atcggcttgc gcattttctt tacgctgctt
    12301 cgccaaaatg gattcttgtt cttggatcga ctcagatagt ttgcctaatt ctagattgaa
    12361 tttcgcaagc tgtgcttgtt tgttgtccca atcttttttt gctgtcttga tttcagcaac
    12421 catcaaagag acatcggttt gagtcagtgt ttcttttagc cgtaaaaact cctttgctgc
    12481 ctcactctgg gcggccaaag gagttaactg ttcttccaat tcgtggatga tgtcttggac
    12541 gcggctgagg ttgtcttctg tttcaaaaag cttctgttct gctttctttt tccgctgctt
    12601 gtatttcaag acaccagctg cttcttcaaa gatcccacga cggtcttccg gtttactact
    12661 gaaaatcgct tctacttttc cttgagaaat gattgagaac gattcttttc ctaatccaga
    12721 atctaagaac aattcttgga tatcttttaa tctgcagctt tgtttattga tgaaaaactc
    12781 gctttcccca gtccgtcgat aacggcgggt tacactgatc tcattgtact ccaatggcag
    12841 ataatgatca ctattgtcta aaatcactgt gacttctgca atattcagct gcttacgggt
    12901 atcggaaccg gcaaagatga tatccggcat ttttccgcct cgcagacttt tagcagattg
    12961 ttctcctaat acccagcgga tagcctctgt aatattgctt tttccgctgc catttggtcc
    13021 aaccactgct gtgacgctat tttcaaaatc aatgactgtc ttgtctgcaa atgatttaaa
    13081 accggctatt tcaattcgtt tcaaatacaa agctttccct cacttacttt tacagtgctg
    13141 ccaatgcatt ttccgccgca gcttgttctg ctaatttttt cgatttccct tgtccagtgc
    13201 caatcaattg atcatctaca taaacttcga tccaaaatac tcgttcatga gctggacctt
    13261 cttcattgat caatcggtat tcgattgata catcacctga tttttgcaag acttcttgta
    13321 gttttgtttt atgatccatc tcatgtgaaa aagcacctgc tttgacttta ggaaaaatca
    13381 ctttttcgat gaatgcatgg gtcgtgtcga atccctggtc aagatataac gcgcctaaaa
    13441 aagcttcgaa taggtcgcaa agcaatgctg gtcgcgtacg tcctcctgaa ttctcttctc
    13501 ccttgcccaa caaaacatat tgatcaaagt gacattcttt ggcaaatttc gacaagctgt
    13561 cttcgcgaac gatcgcagct cgtgtttttg tcaattttcc ttctggcaaa tgaggatata
    13621 aacggaataa atactgagaa accatcagtt ctaaaacggc atctcctaaa aattcaagtc
    13681 gctcattatc cgataattgc agattgcgat gctcattcac ataggatgaa tgggtaaaag
    13741 cttgttcaag caaatttaga tcgtgaaaaa cgatgttata gttttctttt agttcttttg
    13801 tcagctggtt gtccattttc cacattcctt ttatggatat tttttcgata cttccttatt
    13861 atactggttt tttgtcaaat ttgaaatggt tcggtcaaaa ggtaagtaaa gctttaataa
    13921 tcctagtgat tgacgagaag ttgtgaaaaa ttcgtttagt ttcgagtatg aagaaaaaat
    13981 tttgtgaaaa tggctttcca tatttttgca taaatttgac ttaataccga gaaactggac
    14041 tttgaacacc gtttattgga gaagtcatga aatcagcgcc ttgacctgaa tattagagaa
    14101 ataaacagaa aaatatcaaa aaaccgaaaa aggacatcgc cctttttcgg tcatttttag
    14161 tgattatgaa cgtgaatctg ctgtaacaga aacatttgcc catgggtttg gtacatcata
    14221 agaccaatcc cagtctttga cacggtcgtt gactggcaat acttcattac ggaacaacgt
    14281 tggaatcgca aatgcttctt caaatgcata ttgttgccat ttttgatatg cttctttttg
    14341 gtactcttca tcaaatgctt tgcttgaact gatatcgttg atcaattttg tattttcttc
    14401 tgattcataa cgagtgtagt tgaacgcagc atttggtccc catagtccac ttggatttgg
    14461 atcagttcct gtattccaag cagcttggta aacatcaatt tcaggatcat cgttttctaa
    14521 tttatcatag aaagaattga attcgatcag acgacctgtt gtgtattgga catccaaacc
    14581 aatttctttc cattgttgga tatagtaatc tgccaatggt tgcgctgttt ccccaccgga
    14641 catcgaagca aagttgattg ttagtttatt tccatctttg tcttctctta aaccatcgtc
    14701 atccacatct ttgtagccag catcatctaa tagttttttc gctttgtcta gatcttgtgt
    14761 gaatcctttc atctcatcat catggtatga ctggaatact ggaggaatca atgaagtcgc
    14821 attgcttctc aaacctgcat agaaacgttc acctactgca ttattatcta atgcatagcc
    14881 cattgcttga cgtaatgatt tatcagccat ttttgaatct ggattgtatt ccacactctt
    14941 tgtatctgca ttccatttac ctaacttgaa tcctagataa gtataagaag tttgttcacg
    15001 gcctaagttt gtatagccag caatttcttt ataggtatcg taagtatctg ttggcatttg
    15061 gaaaatcata tcatataatt tagagttcaa tgcttcagtt gcacttgcca tcggtacaga
    15121 tttcatcgtg atctttttca atttaggcgt accattccag tagtgctcgt ttggcaagta
    15181 ttctactgat tctcctgcaa cgatattact catgtaataa gggccaaaag tcactggatt
    15241 tttacgtact tgatcgcttg attccatatc tttgattgct acaccttcta acgtatgttt
    15301 tggtgctgca tatgaccaga taccaccacc cgattgaagc atgcttgggt ttacttcttt
    15361 atattcgatt tcaactgttt tgtcgtctat ttttttgatt ccggagattg tgtcggcact
    15421 tccatcatga tattcttcca tacctacgat gttacggaaa gtgtcgtcat aacggatacc
    15481 agtgtaatct ttattcccaa tgatttcgta agggaaaatc acatcgtccg ccgttacatc
    15541 ttcaccatct gaccatttga cgttgtcttt tactttgatt gtcgcttttt tcgtatcttc
    15601 atctagatca agcgttgcag ctccgtcgtc tgtgattctg aaatcgtcat cgtttgcaaa
    15661 tagcggttca tgagaaggag ccataaaagc tgcatcatat gaatcttggt aaaactccca
    15721 ctggaacaat cctttaaact gactgtccgt tgcgaccgca gcttctaatt cgccgtcttc
    15781 gattgcatca tcattatttt ttgttgcaat tggaaaagtt aattcctctt tgttattgcc
    15841 gttgcctcca gagttgtttc cagaggtatc tgtacctccg cccccgcatg ctgcaagaac
    15901 gacagcagac atcagcgctg caaatccaaa aatcgttttc ttcattccta tttccccctt
    15961 cgcttctata ctagcctaac cgttggcgcg catctgcaga acgcttgaat gcttgtccaa
    16021 cataattgat actcaacatc aaaactaaaa taaggattga tgcgggtaac cagatccatt
    16081 gccgattcac taagacttct ccgttacttg cataaccgat caatgtacct aaactaggta
    16141 catttgccgg caagccgaaa cctaagaaag tcaaagttgt ctcgataccg atgtttgccg
    16201 caaagttcat cgtcaaatta gtaatgatta atgaacttag attcggcatg atttcgcgga
    16261 acatgatttt aaaatcattg gttcccattg tttttgaggc actgacataa tctcttcttg
    16321 cttcagataa tgtcttgctt cggaataatc gggctttagc tacccaataa aatgcactca
    16381 tgatgaatat aaaggaccag acattgtatc tggaaataat cgatacgaag acgataatga
    16441 tcagcatgat cggtagaatc atgataaaat caacgatccg catcaagatg ttgtcaatga
    16501 tccctccgta gtagccagag atgatcccta atccaacccc gatgatcgaa gtaaggatcg
    16561 tgatcgcaaa tccgatcgta acagagtttc tagcacctat gatcaattgt cctaatacat
    16621 cacgtccgcc ttcatctgct cccagaatga acttttgtcc ttgtgtcgaa actgttcctg
    16681 gtgcagcgta tttgtcgaag atactaacat acatcacttt gtcttgatcg gtcagcatcg
    16741 caccgataaa gatcactaat aataaaacca ctagcaaacc taatgaaaaa atagcaattt
    16801 tatctttttt aaattctcga gcaatcattc gtatccccat aggaggaata ctttcttgaa
    16861 ctgctgtatt gtttttttca ctcatcgatc cattctcctt tctgattggt tttattgaat
    16921 ccgaatacgc gggtcaacga tactcatgat gatatctgaa agtaatgtcc ccactagcgt
    16981 cgcgattcct aaaatcaata ctaaggatgt gatcacggta tagtcccgtt ggccgatgga
    17041 atcaataaat agttttccga ctcccggata accaaaaatc ttttcgataa ctacagaacc
    17101 agcaatcaac gaagtgattt cataccccat ttgagaagcc atcggcaacg ctgcatttcg
    17161 gaaaatatgt cgactgaata ctttattcgt cggtacccct ttggaacggg ctgttctgac
    17221 aaaatcgagc gatttcgaat cgattacttc acttcgcaga tattggattg tgacggcagt
    17281 cgccaaaaat gcttgggtaa ttgatggtaa cagaatatat ttcaaacggt ctatccattg
    17341 agtgataaat ccaccttgca aacctagtgt ttgtgatcca cttgttggga accagcctaa
    17401 acgataacca aaaacaaata gcatgatcaa gccgaagata aataaaggaa cagcaaaact
    17461 aaagaagttg tagattacga caatcttatc caagattgaa ttttgatacc ggccagcaag
    17521 catccccaga ggcacagcaa tcaaataagt aaggacaact gtcaagatcg atagatataa
    17581 agtgttgatg atccgttcgc cgatcaagtc agcaacagag cgtttgtata agaaactttg
    17641 accaaaatcg ccttgcaaag catttccgat ccaacggaag tactggacat accatgggtc
    17701 attcaagcca gctgcttgtc gcatctgttc gatcactgcc ggatcctgat ttggattgat
    17761 caaaccggta aatggatccc ccggcatcgc ttgggctaat aagaagatca gaacacttaa
    17821 aataataact tgcgggatca tcaataaaac acgtcgcaga atcgttttcc acattagctt
    17881 gcacctccat ctttcaacgc cactttgtgt gtagttgtga tcgtacgcaa atcgtagact
    17941 cgaccagtcg catcatagta ttctttttgg ttttggatat actcttgttc tactcttcgt
    18001 cgattttctt tgtggctttc cctattttct acatcgattt gcggaatagc tgaaagcaga
    18061 cgtttcgtat agatatgtcg cgggtcatta taaatgtctt ctcttgtacc gatttctaca
    18121 aatcgacctt tgtacataat tgcgatattg tcgcacatgt gttttactac acctaaatca
    18181 tgtgaaatga ataaatagct gaggccgtat tcttgctgga ttcgtttcat gaaattcaat
    18241 acttgcgctt gaacagatag atccagagca gatacaggtt catcggctac gatcagtttt
    18301 ggattggtag caacagcacg ggctactcct agacgttgac gttgtccgcc tgaaaattca
    18361 tgaggatatt tatatagcgc atcttccggc atcccaacga tatctagcag tccttttacc
    18421 tttttctttt cttcttgatc gcttaaccgt tcaaaatttc ggatgggttc agcaatgata
    18481 tccaacactc gctttttagg attcaaactg gacattgaat cttggaagat catctgcaca
    18541 tccttgttat aacccatttt tttgcggttg cttcgcttcg ttacatcttt tccttcatac
    18601 atgatttgtc cggctgttac cttttctaat cccacaacgg cttttcccgt agtagatttt
    18661 ccagagccag actctcccac taaaccatat gtctttcctt tttcgataat aaaatccaca
    18721 ccatctacag catatacatg atccgttact cgattgaaga atccactgcg aatagggtaa
    18781 tgaactttca ggtctttgat tgtgattaat tctgtcatct acacctctcc tttttcgtct
    18841 ctaaagtgga aatgcttata gcaggagcaa cgtaccaagt ggttcggtgc gacttcatga
    18901 aggataggct cttcttcatg atcgcttgct ggtatccatg gaatacgtgg tgcaaaccga
    18961 caacctttac gaggcatgtt tttcagagaa ggaacgacac cttcgatcac atggagttct
    19021 gcatcatgat ttccttcttg tggtatagag ttcagtaaag agcgtgtata aggatgctta
    19081 ggatgatcaa acagttcttt tacacctgct acttcaacga actgtccgcc atacatcact
    19141 gccactcgat cagccatttc tgctacgact cctaagtcat gagtgatcaa gatgatccct
    19201 gtttttgttt cttcctgaag gtctttcagt aaatcaagaa tctgtgcttg gatggttaca
    19261 tctagggctg tcgtcggttc atctgcaatg acgattggag gtttgcaggc gattgcgatt
    19321 gcgatgatga cacgttgtct cataccgcct gatagttcat ggggatattg gcgtccaact
    19381 ctcgcaggat taggaatccc tacttgttct agcaattcta gtgcacgagc ttgacgctgt
    19441 tctgcattca tctttgtgtg ataggaaagt ccttctttga tctggtcttc gattctcatg
    19501 agaggattta atgcagacaa cggatcttgg aaaatcattc cgatatcatt tccgcggatt
    19561 ttattataga gcgtttcgtt caggtcgatt aaattcagat cgttatataa aatctcacct
    19621 gtaatttttg tgttattagg atcaagtaat cccataatcg tagtagccaa ggtacttttc
    19681 ccgcacccag attccccaac gattgctaaa atctcgtttt tgtctaaagt aaatgaaacg
    19741 tcatctactg catcgtaata gtcatctttg agtcggaatc ctgtatgtag gttctgaact
    19801 tctaataatt ggttatcgga caatcgaata cccctctctg cttcctaaat ggaagttttt
    19861 cttaataaaa aaggacatta cacagaataa agcgttttat aacaaaggtt attcttatct
    19921 ttgtgtaatg aattattaat aattatatag aaaatcgctt tactttgcaa cataatttct
    19981 gtttatcgcc agaattatca gaaaaatgac ttttacgtgt caaaaagatt aaaaaaagaa
    20041 ggataaaaag ctgtatgcag ctttttatcc ttctttgctt ttatttgaaa ttatttaaga
    20101 tggctgctga tataatctac tgcgccaccg actgtttcaa tttgttcagc gtcttcatct
    20161 gaaatttccg taccgaattc atcttcaagc tctaaaacga attccataat actaattgaa
    20221 tcagctttta aatcatcttt gatgttcaat tcatccgtca ctttatcggt atctatctca
    20281 aaatgatttg aaataatttt cgctactttg ttaaatactt cttcgcgagt caactttttc
    20341 acctccaaga atcatacact tgtcaattac ctacaccatg gctaatttta ctggcatttt
    20401 tcaggaatgt ccaccttttt ttcaatggaa cggattgctt cagatatcca aatactattc
    20461 ttcgcttttt tcaaattgtt cgactaattg tccaactaca tctgtctcaa gcattgtgtg
    20521 gatttggcgg atcgtatagc gaacagcatc ggggcctgtt gctccatgag ttttaatgac
    20581 tggtgctttc aagccaaaca aaacggcacc gccatgtttg gaataatcca tttctgcttt
    20641 caaagaacgt aagccgtctt ttaaaagcat agcgcccatt tttcctttga tcccttcatt
    20701 tagaatagca gattttaata gattcatcat attcattgct gttccttcaa tagatttcaa
    20761 aacagcgttg cctgtaaatc catctgtgac cactacatca gccacaccat tgagtagctc
    20821 acgagcttct acgtttccaa taaaattcaa gcttgtttca ttctgaagga gttcataggc
    20881 ttttttcgtc acttcactac cttttgttgc ttctgttccg ttgtttaaga gtgcgacacg
    20941 aggacgtccg atacctcgga ctttacgtgc ataaaaagaa cctaaaatac cgtattgcaa
    21001 caaatgttct ggtttgtttt ctgcgttagc gccaagatcc agcatatcga aaccggcacc
    21061 ttctttccct actactggca aggtagacat caagccaggg cgttcgatat ttttgatacg
    21121 tcctacgatg aacaaccctg ctgctagtag cgcgcctgta ttccctgctg aaaatacagc
    21181 atctgcttca ccgtttttca ctgcttgtgc agcaaggacc attgatgcag tcttttttcg
    21241 tcgaattgct tttactggct catcatcact attgattttt tcgtctgtat ggataatcgt
    21301 gatgtttttt tcatttgtta agtatttctt gatttctgct tcttttccgt aaagtaagaa
//
LOCUS       DACDHL010000044        21316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDHL010000044 DACDHL010000000
VERSION     DACDHL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.044"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            239..704
                     /locus_tag="IV228_001661"
                     /pseudo
     CDS             239..704
                     /locus_tag="IV228_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1047..1303
                     /locus_tag="IV228_001662"
                     /pseudo
     CDS             1047..1303
                     /locus_tag="IV228_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            1690..1917
                     /locus_tag="IV228_001663"
     CDS             1690..1917
                     /locus_tag="IV228_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8717557.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1933..3099
                     /locus_tag="IV228_001664"
     CDS             1933..3099
                     /locus_tag="IV228_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8717558.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3334..4377
                     /gene="hrcA"
                     /locus_tag="IV228_001665"
     CDS             3334..4377
                     /gene="hrcA"
                     /locus_tag="IV228_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8717559.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4471..5034
                     /gene="grpE"
                     /locus_tag="IV228_001666"
     CDS             4471..5034
                     /gene="grpE"
                     /locus_tag="IV228_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8717560.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5085..6914
                     /gene="dnaK"
                     /locus_tag="IV228_001667"
     CDS             5085..6914
                     /gene="dnaK"
                     /locus_tag="IV228_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8717561.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7065..8231
                     /gene="dnaJ"
                     /locus_tag="IV228_001668"
     CDS             7065..8231
                     /gene="dnaJ"
                     /locus_tag="IV228_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8717562.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8599..9336)
                     /locus_tag="IV228_001669"
     CDS             complement(8599..9336)
                     /locus_tag="IV228_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8717563.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9338..10840)
                     /locus_tag="IV228_001670"
     CDS             complement(9338..10840)
                     /locus_tag="IV228_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8717564.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11099..13093
                     /gene="uvrB"
                     /locus_tag="IV228_001671"
     CDS             11099..13093
                     /gene="uvrB"
                     /locus_tag="IV228_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8717565.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13103..15919
                     /gene="uvrA"
                     /locus_tag="IV228_001672"
     CDS             13103..15919
                     /gene="uvrA"
                     /locus_tag="IV228_001672"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8717566.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16182..17447
                     /locus_tag="IV228_001673"
     CDS             16182..17447
                     /locus_tag="IV228_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8717567.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17451..18182
                     /locus_tag="IV228_001674"
     CDS             17451..18182
                     /locus_tag="IV228_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8717568.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18317..19201
                     /gene="rapZ"
                     /locus_tag="IV228_001675"
     CDS             18317..19201
                     /gene="rapZ"
                     /locus_tag="IV228_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8717569.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19198..20196
                     /locus_tag="IV228_001676"
     CDS             19198..20196
                     /locus_tag="IV228_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8717570.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20230..21165
                     /gene="whiA"
                     /locus_tag="IV228_001677"
     CDS             20230..21165
                     /gene="whiA"
                     /locus_tag="IV228_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8717571.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
BASE COUNT         7086 a         3664 c         4619 g         5947 t
ORIGIN      
        1 tggtatttta caatataccg gtatggggct aaaaggggat caagtattgg ataagaatca
       61 aaataaagtt ttagccaatt caaaaagtaa ttttgtaaag attcatttat ttgaaacatt
      121 taaaccaaaa gaacacacat atctgggaga agtatattta gcaggccaaa tatatactgt
      181 taatgaaaaa gatagttcgg gcaacagcag aaaagtatat aaatttccat tagctttaat
      241 aaatcaagag caattgattg aagataaaga tatataacca ggaagaaaat caaactagac
      301 atattcgtaa tttatctaat gctaaattag aggaagaagc aagaaaagtt agtaattata
      361 ataaaaaaat ggctgcaaaa tatgttaacg aagaggagtt ttgcttctca aaaacaaaaa
      421 tatataaacg taatcccttc gtatctgaat atgttaaacg acttgcaaaa ggtatttgtc
      481 aactttgcca agaaaaaggg ccatttataa aagacggtgt accttattta cattgtcatc
      541 atatagaata tttgtcgcaa ggtggaaaag atgtgattga aaactgtatc gcgttatgtc
      601 ccaattgtca tgctagaatt catgaacttg agttacctga cgataaagaa aaattgctga
      661 agatcgttga aaaaagagaa aatagctttt ctaaaatacc gtaatttatt tttataggac
      721 aaataagcta atgtgagcta tgccttctat ataagactaa gttctattat aagtaacgaa
      781 gtaattctcg ttccttcctg tagtaatatt ttttaaataa cctataggct tcttcaaaag
      841 tacatcctcc agtaaattct aagtactgga ggattctttt tctagtgttg ctttactcat
      901 gaaaaagtag ttggctactt catcaaaatt tctgaacttt tttaatgtat aataaagagc
      961 tcgatcattt actacaagga tgctagctcc tacattttca cagtttttta gcaagtaaat
     1021 ttttctaaaa tcacatcagg aaatgcatga gattctttaa gagaggcaat tatagggcta
     1081 tgattacggt tcgtgtaagt gacgccaaaa aagaatggct ttcttatatg gctgaatttt
     1141 atgggattac tttatctgaa cttctaaaaa actattcgat ggaacaacta gaagatgaat
     1201 acaatcgtca aacggctgaa attgcccata aacattggat aagaagtggg aaaacaacag
     1261 tattcatgga agaatcctta atgaatttgg tgggctggaa tgacctgccg tttaaaattc
     1321 acgcccgaaa ttcagaagca gctctgattg gcaaatacgt gtattggtag agatagaaga
     1381 taatcaattc gttgttatcg ctattcaagt agggcaccga agaaatgtct atgattaaga
     1441 gactgtttat ttttacaaaa aaataataat tggaatctag ttaaaactct tgaagtgaga
     1501 gatttgacta gattttttta tgaataaaat aaggacaaaa ttgtttagga agaatcatga
     1561 gtgtaaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
     1621 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
     1681 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
     1741 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
     1801 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
     1861 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
     1921 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
     1981 catatcccat tttgcgagca tatctgctat tactgtgact ttaataaagt ttttttagaa
     2041 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgagatt aatgctggaa
     2101 agtcatccta cagcgaattt ggaaacactt tatgtaggag gcggaacacc aacttcttta
     2161 tctgctaaac aacttgatcg actattatca ggtgctagag agatcctgcc ttttcaaaat
     2221 ggtaatgagt ttactgtaga agcgaatcct ggtgatttga cccgtgaaaa acttcaagtg
     2281 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga caatcgctta
     2341 ttgaaaaaaa ttggacgtaa acatactgca gaagatgtgt atcaaacaat gcaattttta
     2401 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
     2461 ctagaaggaa atcgggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
     2521 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
     2581 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
     2641 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
     2701 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
     2761 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
     2821 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
     2881 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
     2941 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
     3001 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacttttttg
     3061 gggaacaatg tgtttgagcg ctttttattt gaaaaatagt ttgaagaata tttgtttttt
     3121 atcgaaactt gaaaagaaaa agtttagaaa gtttgacaat gaggtattga agtattgata
     3181 taactggatt tacatcaatt atttagaatt agcactcttg tgtttagagt gctaaaaaat
     3241 ggagtctttc tcttgacagg agaagggctt cttgctatat ttataagtga attagcactt
     3301 agaatatatg agtgctaatg agaggtgagg aagatgttaa caaagcgaca aaacgacatt
     3361 ttacgtctga tcatccaaca ctatacgagt agtggcgtgc ccgttggttc caaaacatta
     3421 atgtctgagg gagtcaaggc cagctcagca acgattcgca atgatatgaa agcattagaa
     3481 gatgaaggat tgctgttgaa gactcactct tcttctgggc ggattccatc tgctttaggc
     3541 tatcgttatt acgtggatca tttgttaaaa cctgctcgcg tagcagaaga tgatttgctg
     3601 ctgattcgtc agtctctagg cagagaattt catgaaatca atgagattat taagcaatct
     3661 gctgagatat tatctgagct gacaagctat acgacttttt cattgggacc tgaaataaaa
     3721 gaccgccgac tgactggctt tcgtatcgta cccttgaata atcgacagat tattgctatc
     3781 gtcgtaacag atatggggaa tgtagaaagc caagtgttca ctcttcccga atatatggga
     3841 agtcaggaac tagaaaaaat ggtacagatt gtaaacgacc gtctagtcgg agatcctctg
     3901 atgacggtgt accataaact tcgtacggag attccgatga ttcttcataa atatttccag
     3961 acgacagaag ggatgtctca gttatttgac accgttcttg gtcatgtttt tgaagaaaag
     4021 gtttatgtca gtggacaaat gaatctcttg gattatgagc cccgtcaaga tcttgatcag
     4081 ttcaaatcga tgttttcttt tatgaaagac tcagacggac ttacacaaat gatcactccg
     4141 atggatagca atattcatat caagattggt gctgaattag gcaatgattt attgcagaat
     4201 atgagtatga ttcaagcaag ttacgagata gatggtcatg gaagaggtac aatagccttg
     4261 ttagggccta ctagtatgcc ttattctcgg atgttcggtc tagtcgatgt ttatcgaaga
     4321 gaactagcag ctaaacttgc tgattactac cgctcgcttg acttgtcaaa ctcgtaatgt
     4381 gaataacgaa gctatttgca gctgatcgtt ctctctttct tagtaagagc aatcaaacag
     4441 ctgactgaat aatgaaagga agactgtatc gtgaaagaaa atcaagaaga actagataaa
     4501 gaaatgacag atgctcagcc agaaccggaa atcgatgtcg aagctgcaga agattcgggt
     4561 atttcagaag ctgaagcgga agaattcgaa acagcgaaat taaaagctga gttagaagaa
     4621 atggaagata aatacttgcg ggctcgtgct gaaatcgcaa atatggcaaa tcgaggaaaa
     4681 aatgagcgtg agcaattaca aaaataccgt tcacaagatt tagccaaaaa attacttcca
     4741 tcaattgaca acctggaacg tgcattagcc acagaagtgt cagatgatca aggggcaggt
     4801 ttgaaaaaag gcgtggaaat ggtcttggaa agtttgcgta acgcactgga agaagaagga
     4861 attgaaaaaa tccctgctaa aggagaagcc tttgatccta atcttcatca agcagtacaa
     4921 actgttccag cgactgaaga tacaccagcc gatacgattg tagaagtact acaagaaggc
     4981 tacaaattac atgatcgcgt gttaagacca actatggtca ttgttgcaca ataattgtgc
     5041 ggatataaaa cttacacttt caattaaaga aggagacgtt aattatgagt aaaattatcg
     5101 gtattgactt aggtacgact aactctgcag tagcagtatt ggaaggaaac gaagcaaaaa
     5161 tcatcgctaa cccagaagga aaccgtacaa caccttctgt tgtatcattc aaaaatggag
     5221 aaatccaagt aggggaagtt gcaaaacgtc aagctgtgac aaacccaaat acgatttcat
     5281 caatcaaacg ccatatcggt gaagcaggtt acaaagtaga tgtagaaggt aaatcatata
     5341 caccacaaga aatctcagca atgatccttc aatatatcaa aggattcgca gaagactatt
     5401 taggtgaaaa agtcgaaaaa gcagttatca ctgtgccagc ttactttaat gatgcacaac
     5461 gtcaagcaac aaaagatgct ggtaagatcg ctggtctaga agtagaacgt atcgtcaatg
     5521 aaccaactgc agctgctctt gcctatggtt tagataaaac agatcgcgac gaaaaaatct
     5581 tagtattcga ccttggtggc ggtacttttg acgtatctat ccttgaacta ggagacggtg
     5641 tctttgatgt attgtcaaca gctggcgaca accatctagg tggggacgac tttgataaca
     5701 aaatcatcga ctacatggta gcagaattca aaaaagaaaa cagcattgac ttgtctcaag
     5761 acaaaatggc tttacaacgc ttgaaagacg ctgctgaaaa agcgaaaaaa gatttatctg
     5821 gcgtatcaag cacacaaatc agcttgccat ttatcacagc aggagaatct ggaccattgc
     5881 atttggaaat gacattgaca cgtgcaaaat ttgatgaatt aacagctgac ttagtagaac
     5941 gtacaaaagt accagtacgt caagcgttaa aagatgcagg tctgtcacaa tcagaaatcg
     6001 acgaagttat tttagtcggt ggatctactc gtatccctgc agttgtagaa gcagtacgta
     6061 aagaaacagg aaaagaacca aacaaatcag ttaaccctga tgaagtagtt gctatgggtg
     6121 ctgctatcca aggtggcgtg atcactggtg atgtgaaaga tgttgtttta cttgacgtta
     6181 caccattgtc attaggtatc gaaacaatgg gtggagtgtt cactaaattg attgaccgca
     6241 atacaacgat tccaacaagt aaatcacaag tattctcaac tgcggcagat aatcaaccag
     6301 cagtagacat ccatgtatta caaggtgaac gcccaatggc tgctgacaac aaaactctag
     6361 gaagattcca attgacagat attccagcag ctcctcgtgg tattccacaa atcgaagtaa
     6421 cattcgatat tgataaaaac gggattgtaa atgtatctgc gaaagatcta ggtacacaaa
     6481 aagaacaaaa aatcacgatc aaatcttctt caggtctaac agacgaagaa atcgaacgta
     6541 tggtgaaaga tgcagaagca aacgctgaag cagataaagc tcgtaaagag gaagttgacc
     6601 tacgtaatga tgtagacgca ttgctattct ctgtggacaa aacattgaaa gaattagaag
     6661 gcaaagtgga cgaagaagaa gtgaaaaaag cagaaacagc tcgtgacgaa ttaaaagccg
     6721 ctgttgaagc aaacaacatc gaggaaatga aaactaaacg cgatgcattg aacgaaatcg
     6781 ttcaaaactt gactgttaaa ctatacgaac aagctgcaca acaacaagct caagaaaatc
     6841 cagaagcagc tcaaggcgga gcagatgatg tcgttgatgc tgattttgaa gaagtcgatg
     6901 gcgacgataa ataatcagtt cactgaattt cgagagaagg ccaaagttgc tactttggct
     6961 ttttcttttg aaaaatagtt tgaagtatcg gtttttgttt caaacaaaat atgatatgat
     7021 aacaagcgac gtgaaaatga tgagaaccaa taggaggaaa gcctatggca acaaaacgtg
     7081 attattatga agtcttaggg ctctcaaaag gagcatcaga agatgaaata aagaaagctt
     7141 atcgtaagct ttcgaaaaaa taccatcctg acatcaataa agaacctgat gcggaagaaa
     7201 aattcaaaga agtatcagaa gcatatgaga ttttaagtga cccgcaaaaa cgcgctgcat
     7261 acgaccaata cgggcatgca ggcacagatc caaattacgg tgctggtgga gctggtggcg
     7321 gatttggtgg attcggcggc ttctctggtg gcggcttcgg tggttttgaa gatatcttcg
     7381 actctttctt tggaggaggc ggtcggtcag tcgatccaaa tgcgcctcgt caaggtgctg
     7441 atttgcaata tacggtcgac ttgtcttttg aagaagcgat ttttggtgta gaaaaagaaa
     7501 tcaagtataa tcgggaagaa atctgtcata cttgtggtgg aaacggtgca aaaccaggca
     7561 cacaaccaac tacttgtcat aaatgtcatg gtgcaggtac gatcaatgta gaacgccaaa
     7621 cgccacttgg acgagtgatg agccgtcaaa cttgtgatgt ttgtcatggc acagggaaag
     7681 aaatcaaaga accttgtcca acctgtcatg gctcaggaca tgagaagaaa gcacattcag
     7741 ttaaagtgaa tgtaccagct ggtgtagaag aaggacaaca aatgcgttta gctggtcaag
     7801 gagaagctgg agaaaacggc ggaccatttg gtgatttgta tgtggttttt cgtgtagaag
     7861 aaagtgatat ctttgatcgt gaaggttctg aaatttacta tgaattgcct ttaagttttg
     7921 tacaagcagc tttgggggat gaagtccaag taccaactgt ccatggagat gtgaaattga
     7981 agatccctgc tggaacacag acaggtacga acttccgttt acgtggaaaa ggcgctccga
     8041 agttacgtgg tggtgcaaca ggcgaccaac atgtcaaagt aaaactgatc acaccgaaaa
     8101 atctaaatga acaacaaaga gaagcattac gtgcattcgc tgaagctggc ggcatcaaag
     8161 ttgaagaaca gcaagaagac ggtttcttcg ataagtttaa agatgctttt ggcgggaaaa
     8221 agcggaagta gtttttctag taaaaatata aagtagactt gcccaaattt tctatttcta
     8281 gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatacg ttcgcctggt
     8341 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata agataatcat
     8401 catagtttct gaaggtggta tcgactatgt acttgatatc gtcaatgaac cgatggtact
     8461 ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggagaac aagcgatgcg
     8521 gctggtacag ttaagtgaaa aaacaagaaa ctaaaaatgc ccggtcgtca tgaagcgacc
     8581 gggcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc
     8641 attttgcgga ttttcaaaga tttgttgagg cgttccgtct tctaaaaagt ttccgtcgtc
     8701 aatgaacatg acacgattgg ccacttcttt agcgaagccc atttcgtgcg taacgataac
     8761 catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc
     8821 ggggtcgagt gcagatgtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc
     8881 acgagcaata gccacacgct gcttttgtcc accggaaagc atttcaggat acatatcttt
     8941 tttatcttct agaccaactg tctgcagcaa tttgattgct tttttctctg cttcttctct
     9001 agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg
     9061 gaataggtta aagtgctgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt
     9121 gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa
     9181 acgattcaag caacgcaaaa atgtactttt tcctgaacct gaagggccaa tgatacagac
     9241 gacatctcct tcgttgatgg acacattgat gtcgtttaat actgtgttat cgccgtattt
     9301 tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa
     9361 cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt
     9421 aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga
     9481 ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga
     9541 atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat
     9601 aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc
     9661 ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa
     9721 ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa
     9781 gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag
     9841 cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa
     9901 aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt
     9961 tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg
    10021 aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt
    10081 tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg
    10141 catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg
    10201 cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt
    10261 cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt
    10321 cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat
    10381 cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa
    10441 agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa
    10501 tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt
    10561 ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc
    10621 cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa
    10681 ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg
    10741 ccgttgtagt gttgtcagct tgtgcaacat gtccaaacgc aagtgtcaca agtcctgtca
    10801 ttaagaaaac aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc
    10861 aatattttat tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa
    10921 tgaaaaaaat gcgagtttct aacattttat gttataaaac gtaacatgca taataagata
    10981 aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt
    11041 tccttttctt tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat
    11101 gattgagaga gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga
    11161 tcagcctgaa gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat
    11221 cttattaggt gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt
    11281 gaataaacca acactaatta ttgcccataa caaaacattg gctggtcagc tgtacgggga
    11341 attcaaggaa tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta
    11401 tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa
    11461 tgatgagatc gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt
    11521 gatcgtcatt gcatccgttt catgtatttt tggattaggt tcgccttttg agtaccaaaa
    11581 acaagtagtt tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt
    11641 agtcagtatc caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg
    11701 cggtgatgtc gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt
    11761 ttttggagat gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg
    11821 agaaacggaa cacgtggcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat
    11881 ggagcatgcc gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa
    11941 tgaaaacaaa cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat
    12001 gatgttagaa atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg
    12061 taaagaaggt gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt
    12121 ggcggacgaa tcgcacgtaa ccatgcctca agtcagagga atgtataatg gtgaccgagc
    12181 gagaaaacag atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc
    12241 acttcggtta gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc
    12301 aggaccatat gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg
    12361 gctgcttgac ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg
    12421 cgaaataaat gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa
    12481 aatggcagaa gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca
    12541 tagtgatatc aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt
    12601 tgatgtgtta gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct
    12661 tgttgctatc ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca
    12721 aacgattgga cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt
    12781 aacagattct atgcgtctag cgatggatga aacatcaaga cgccgtacga ttcagcagaa
    12841 gtacaatgaa gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat
    12901 ttctattacg aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtatgaaga
    12961 aatgacgaaa gaagaaaaag atacgttatt gatgaaatta gaaaaagaaa tgaaaaatgc
    13021 ggcgaaagca ttggattttg aaacagccgc taatgtgcga gatatgattt tagaattaaa
    13081 agcttcaaag taggaggaca acatggctaa cgataaaatt acgattcacg gtgcacgtgc
    13141 ccataacttg aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg
    13201 tttgtccggt tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg
    13261 gagatacgta gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc
    13321 ggacgtagat agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag
    13381 taaaaatcct cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt
    13441 gtttgcacgt gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc
    13501 agtagatcaa atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt
    13561 ggcaccgatc gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa
    13621 agagggatac gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga
    13681 gctggagaaa aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga
    13741 agggattcgt tctcgtctat ttgattcttt agaggcggct ctgcgattgg cagacggcta
    13801 tgctctggtt gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc
    13861 ttattgcgga tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt
    13921 tggagcttgt ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt
    13981 gattccagat agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag
    14041 ctctcagtat tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga
    14101 tacaccattc gaagaattac cggaagagca tcaggatatc atcttgaatg ggacagatga
    14161 tcttttccat ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt
    14221 cgaaggggta ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg
    14281 tgagcaaatg cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt
    14341 gaatccccaa gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt
    14401 gtctattcaa aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt
    14461 aattgccaaa ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg
    14521 actagactat ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg
    14581 aatcagattg gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga
    14641 accatcgatc ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat
    14701 gcgtgatctt ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc
    14761 agattatctg attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc
    14821 aggcactcct gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg
    14881 gaaaaaatca atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat
    14941 cactggtgct tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt
    15001 tgtagcagtc acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa
    15061 gaaatcgtta gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat
    15121 ttccggctac gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac
    15181 gccgagaagt aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc
    15241 tcaaacgaac gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa
    15301 aggcggtcgt tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt
    15361 gcctgatgtc tatgttcctt gtgaagtatg tcatggcaaa cgatataact ctgaaacatt
    15421 agaagtgcat tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaagatgc
    15481 tgtagaattc ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt
    15541 tggcttaggt tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca
    15601 acggatgaaa cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact
    15661 agatgaacca acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca
    15721 aagattagta gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa
    15781 aacagcagat tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct
    15841 tgcgactgga acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt
    15901 gaaaaaaata atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa
    15961 tgaatttttg cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc
    16021 tacactgatt ggtatagaca aaaacttcct ataagttcaa tccgttcaaa aaagaattga
    16081 gcaatcggag gagtttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag
    16141 ttggaaaaga aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa
    16201 atgaagaatt tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt
    16261 cttttaatga agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta
    16321 cccgctacag taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtgt
    16381 ttatcgagac gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc
    16441 ttttgattgc ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt
    16501 cagaatattt tatctgtcca tatattgatt attctttatt tgaaagactg atcaataaag
    16561 taagctttta tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca
    16621 gagaagaaat gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg
    16681 ctttgaaacc tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag
    16741 cttttatttt agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg
    16801 gttataaaag tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg
    16861 tgctaaatgc gtatgtagat gaagatcatc aagttcggat gatgtgtctt ggtcatccac
    16921 tgttagaaga tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata
    16981 acgaaaaaat ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg
    17041 ccaatttcga tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt
    17101 tgagacaagg tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgtttcg
    17161 tcacagaaga tcgagtgttt aacaaaccct ttgttgaaac gacctatgga acgaatcaat
    17221 cagataaagc aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg
    17281 aaaatgaaga caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg
    17341 tgttttatga aaaagaccgg tcaatcaagc gctggttgtt gatgaaatat atgttccata
    17401 actatattcc aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt
    17461 ttttcagtat tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca
    17521 accaccgaac gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg
    17581 caacagttcc tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt
    17641 ttctgctgga tgatattgag aaacaaaatt tattgcagcc gaacttcatt gtgttgactt
    17701 gtaacaccgc acactacttt tttgaagaat tacaagcggc aacggatatt cctatccttc
    17761 atatgccaag agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga
    17821 ttctaggaac agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg
    17881 gattcgaaac agtgattcct gatactgttc ttcaagaaaa aatcaattat ctgatctatc
    17941 atgagatcaa ggaatcggat tatttgaatc aagagctata ttacgaaatc ttagaagaag
    18001 cggtagagcg cctaaactgt gaaaaagtta tcttaggttg cacagaattg tcattgatgc
    18061 atgaatttgc tgaagataac cattatccag tgatcgatgc ccaatctatt ttagcagatc
    18121 ggacgattga acgagcgcta gctgaaagaa gcaaggcatt ggatacagca tcggaaaagt
    18181 aaaaaaattg gatttggggc aaatatccta aaagatattt ggtccaaatc tttttgtaaa
    18241 agaaaaagaa aactggtaat gatacgcccg gaatgatata attttactat gcacaatgtg
    18301 agaggagttc gtaattatga cagataattt gcaattagtc atcataacag gtatgagcgg
    18361 tgcagggaaa acagtagcta tccaaagttt tgaagatatg ggctattttt gtatcgataa
    18421 tatgccgcct cgattgattc caaaattctg ggaattaatc aaagaatcag gcaaagtcac
    18481 aaaaatggct ttaggagtgg atttacgttc tcgttccttc tttgaagaaa tccaaaacat
    18541 gctgatcgaa atcgaaaata cgaacttcat cgacacttct atcttatttt tagatgcttc
    18601 agacgaagaa ctagtggctc gctacaaaga aacaagacga gcacatccaa tggcgatgga
    18661 cggtttgatc actgaaggga tacgtaaaga acgggcgata ctagacgact tgaaagccca
    18721 agcttccgtg atcattgata cgacgacatt gacaccacga caattacgtg aaaaaatcaa
    18781 tgagtcattc aaatcaaagg gagaccaagg atttcggatc gaatttgtat catttggttt
    18841 taaatacggg ttgccgatcg attcggatat tgtgatggat gtgcgttttt tgcccaaccc
    18901 tcattatatc ccggaactaa gaccattgac cggaaaagat cagccagtct atgattatgt
    18961 gatgtccttt gatgaaacgc aatcattcta ccataaattt ttggatcttc tggaaactgt
    19021 attgcctgga tacattaaag aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg
    19081 cggtcaacac cgttctgtgg cattgactga acgtttggca aatgctttag ggaaaaacta
    19141 taaagtcaat gtgacacatc gggacaagga taaaaggaaa gagacggtga accggtcgtg
    19201 aaaatgaaaa cctaccggat aagaaaacca aagatcgtag tggtcggcgg tggcactggt
    19261 ctgccagtta tcctgaaaag tttgcgaaat caaggagcag atatcacagc agtagtgact
    19321 gttgcagatg atgggggaag cagcggtgca atcagagaat cgatcgctat ggctcctcct
    19381 ggtgacttga gaaatgtctt agtggcgtta tcggatatgc cacaattcta tgaagatatt
    19441 ttccaatacc gtttcaaaaa ggaagatcag tttttagcca atcatacgat cggtaatctg
    19501 atcattgccg cagtttccga gatgcgggga agcacttatg aggcgattca attattagca
    19561 aaaatgatgc atgtagatgg acatgtttat ccttcttcag aaactccttt gaccttgcat
    19621 gctgtcttta aagacggaac gactgcagta ggagaatcta aaatcgcgat cgaccgtaaa
    19681 accattgacc gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa agcggctcga
    19741 aaagtcgttt cagcaattat ggacgccgac atggtcgttt tgggacccgg aagtttattc
    19801 accagtatcc ttccaaattt agtgatacca gaaatcggcg aagcaatgct caagacaact
    19861 gctgaaaccg tctatatctg taatatcatg acccaaaaag gggaaacgga acatttcaca
    19921 gatgccgatc atatacgtgt tttacacaag catttgaatc agccttttat cgatacagta
    19981 ttggtcaata cggaaaaagt gcctgaagat tatatggacc cagagatcta tgatgagtat
    20041 ctagtacaag tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg agtgatttcc
    20101 accgactttt tggaattgaa agatggtggt gtcttccacg atggagacaa agtagtagaa
    20161 gaattgttcc gtttagtttt cggttcaaaa tattaagcaa tttattgttg gagaaaggag
    20221 gagaatagga tgtcatttgc tgcagatgtg aagaaagaac taactggttt ggaagttcat
    20281 cgagaacatg cgaaagcgga attagctgct ttgctgcgaa tgaacggctc tttaggtatt
    20341 gtgaaccagc agtttatttt gaacgtccaa acagaaaatg ctgcaattgc cagacgtatc
    20401 tattctcttt tgaaagatca ttatcaggta cgaagtgagc tactcgttcg taaaaaaatg
    20461 aaattaaaaa agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa agatgtgttg
    20521 atggatcttg atatcatgga tggtatgatg ttcaatggac acgtttcgga cgagattatg
    20581 ggaaattcgc aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc aggttcagtc
    20641 aataatccgg aaacgagccg ttatcatttg gaaatatttt ctatttatga acagcataac
    20701 caagacattt gcgatatgtt aaattattac ggtctacatg ctcgaacgct tgaacggcgc
    20761 aatggctata tttcttattt aaaaggagca gaaaaaattg ctgatttctt aacattgatt
    20821 ggtgcaacca actcaatgct ccgatttgaa gatgttcgaa tcgtacggga tatgagaaat
    20881 tcggtcaatc gtctagtcaa ttgtgagaca gcaaatctga acaagacgat tgatgcggct
    20941 tctaaacaaa tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca tgcccttcct
    21001 gaaaaacttc aggaaatcgc agaacttcgg cttcaacatc cagaggtcag cttgaaagaa
    21061 ttaggggaaa tgattccctc aggtgcgata tcaaaatcgg gaattaatca ccgaattcgc
    21121 aaaataaatg aatttgcgga aaaactgcgt gaagaagcag tttaatccaa agaaaaaact
    21181 gatacatgaa gcaggagatg cgaatgtatc agtttttttt aaaagttata cttgcttttc
    21241 ttccgtcaaa ctataattcc atctttactg gagtaacata atttggaaag gcttctattg
    21301 tttttaatac aatcgt
//
LOCUS       DACDHL010000045        19541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDHL010000045 DACDHL010000000
VERSION     DACDHL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.045"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="IV228_001678"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="IV228_001678"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8717572.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="IV228_001679"
     CDS             complement(1173..1868)
                     /locus_tag="IV228_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8717573.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="IV228_001680"
     CDS             complement(1881..2597)
                     /locus_tag="IV228_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8717574.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="IV228_001681"
     CDS             2927..3532
                     /locus_tag="IV228_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8717575.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="IV228_001682"
     CDS             3857..4180
                     /locus_tag="IV228_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8717576.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5730
                     /locus_tag="IV228_001683"
                     /pseudo
     CDS             4362..5730
                     /locus_tag="IV228_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(5860..7161)
                     /locus_tag="IV228_001684"
     CDS             complement(5860..7161)
                     /locus_tag="IV228_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8717577.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7391..9382)
                     /locus_tag="IV228_001685"
     CDS             complement(7391..9382)
                     /locus_tag="IV228_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8717578.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9526..9792)
                     /locus_tag="IV228_001686"
     CDS             complement(9526..9792)
                     /locus_tag="IV228_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8717579.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10302..12428)
                     /gene="pnp"
                     /locus_tag="IV228_001687"
     CDS             complement(10302..12428)
                     /gene="pnp"
                     /locus_tag="IV228_001687"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8717580.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12616..12885)
                     /gene="rpsO"
                     /locus_tag="IV228_001688"
     CDS             complement(12616..12885)
                     /gene="rpsO"
                     /locus_tag="IV228_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8717581.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13136..13699
                     /locus_tag="IV228_001689"
     CDS             13136..13699
                     /locus_tag="IV228_001689"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8717582.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13952..14473)
                     /locus_tag="IV228_001690"
     CDS             complement(13952..14473)
                     /locus_tag="IV228_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8717583.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14476..15318)
                     /locus_tag="IV228_001691"
     CDS             complement(14476..15318)
                     /locus_tag="IV228_001691"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8717584.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15311..16036)
                     /locus_tag="IV228_001692"
     CDS             complement(15311..16036)
                     /locus_tag="IV228_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8717585.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16212..16508
                     /locus_tag="IV228_001693"
     CDS             16212..16508
                     /locus_tag="IV228_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717586.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17076..18668
                     /locus_tag="IV228_001694"
     CDS             17076..18668
                     /locus_tag="IV228_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8717587.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18681..19421
                     /locus_tag="IV228_001695"
     CDS             18681..19421
                     /locus_tag="IV228_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8717588.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5587 a         4006 c         3713 g         6235 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatgcg ttcgatgaaa caattgaaac acatgggtga agaggaacat
     5701 ttcacattag aacatgcaga agcaagatag ccttgattaa aagaaatcgt tgcggctaaa
     5761 aatgaaaact tttattgaaa atatcttctt cgtttaccga aaacaggttt tttagataac
     5821 aaaaaggctg tgacaaaaaa tgtcacagcc ttttttcttt tattctattt ttctttccgt
     5881 ggactctact ttgaaataat cgatatgtgc atgctggtat tctaataccg tgaaacggaa
     5941 tgatgcaatc tcaatgattg ttcctgcttt tatttcttta tatcggctag aagcaaagcc
     6001 cgcaagtgta gtaaaattcg tttcttcaaa ctctggaatc tctacatgaa aataacgctg
     6061 gaaatcatct aatgttagtt tgccggagat gatataagag ccatctgact gttccgctat
     6121 caatgcttcg tccaaatctg tctcatctcg gatttccccg acgatttctt ctaaaatatc
     6181 ttcaatcgtc actaagccgc ttgtccctcc gtattcatct tttaagatag caatatgctt
     6241 tccctttttt cgcatgatcg tcaataattt ttttataggg atcgtttcag ttgttacgat
     6301 cggctcttca atcaactgtt ctactgtcag ctggtcatct ttataagatt gcttgatcaa
     6361 atctggcaag gtgacataac ctaaaacatt gtcttttgat tctaagataa ccggatagcg
     6421 agtatgtcct tgttcaatcg aagcttttat cgcttctttg acggtttctg aagcttcgaa
     6481 tacttgcatg gacgtacggt ctacttgtat atcttttgcc aaggtatcat caaattcaaa
     6541 cacgttttcc aaataatgat attcttcttt ggtcaactct cctttttcat aagaattcaa
     6601 tgcgactaat gtcagctctt cttgagacaa tgtctcttct ccttcagata cgagacgcac
     6661 acctaatagc ttccctagcc ctgcagctga atgattcaat acccaaataa aaggaaacat
     6721 tactttataa aaataatgaa gtggtttaac tacgaacaag acgaccttct ccgtattgac
     6781 gatactatag ctcttaggaa gcagttcgcc aataaccaca tcgatatacg taactaaaat
     6841 aaatcccagc gccacagaga ttgtactgct aatagccgaa ctaaaaggta gtttaccgat
     6901 cactggttct agtaaagccg cgacagatcc ttcaccgacc cacccgatga tcaaaccggt
     6961 tagcgtaatt cctaattgac tagctgataa atagttatct aaatggtgtg taacatgaag
     7021 agctagcttg gcgttcttta cattttcctt ttctagttgt tccaatcttg atcgtctgat
     7081 tttcactaaa gcaaattcac ttgctacaaa aaatgaagta attgcaatca tcaaaaataa
     7141 aataatgaaa ttgataacca tattctcccg cctttctctt tttctattgt accctttttc
     7201 tacaaaaaga ttgaaaataa tgcgtattca tatttaggaa taaaaaagga gatgcggcat
     7261 ttttcagcca catctcctta tcacttcaaa atcattgcgt tacttgttgc taaacagctt
     7321 tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag ttcaggtcaa
     7381 agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca atgataagcg
     7441 gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct ggcgaggtag
     7501 cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt cctagagttg
     7561 tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact tctcgccctg
     7621 gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc aaatcatgga
     7681 tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag aacagattca
     7741 cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta tatggctcat
     7801 ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt ttatctaaaa
     7861 tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc aagaagacca
     7921 catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc tgcgggatat
     7981 aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct tgttcccaaa
     8041 taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg cctcggaaga
     8101 atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa aaggtaacta
     8161 aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc agtgctgttg
     8221 tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga tgagacgttc
     8281 ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact gctgggatat
     8341 acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc agtttcaact
     8401 tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa gcaatgactg
     8461 cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc atttggaaga
     8521 atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc aacaaccacg
     8581 cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag ctcagacgaa
     8641 tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct gtcgtagcca
     8701 ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa agcagctgga
     8761 tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag ttcattaatc
     8821 ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct tttccgacaa
     8881 tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa gtgataatga
     8941 cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct cgcaagcctt
     9001 caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt aaggcagctc
     9061 ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt gtgtagagtg
     9121 agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca tatttgattg
     9181 ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca aaatcaggat
     9241 tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa agcggacttg
     9301 tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt gatatctttt
     9361 tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga tatcctttag
     9421 gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta aattgcttct
     9481 aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt ctttctttgg
     9541 acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa ttgcacccaa
     9601 taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta gtaaaaattc
     9661 gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc ccgggacttc
     9721 ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata acgaccataa
     9781 aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt ttacgcttta
     9841 ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag ttgtaacaaa
     9901 agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag aggttgtgaa
     9961 atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct catattttgc
    10021 cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg agaggttgta
    10081 aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc ctaatgtttt
    10141 taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    10201 aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat tgtccccctg
    10261 tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc ttcatttttc
    10321 gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc ttgtttatcg
    10381 atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc gactttattg
    10441 acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt gatcaagttg
    10501 acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc accaactttg
    10561 acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc tgcatcagct
    10621 gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt ttcatcgatg
    10681 attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc aggttcgatc
    10741 tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc agcaatcgtt
    10801 gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa tgcttcagtt
    10861 aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt gataccatct
    10921 tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc ttggatatca
    10981 gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc ggcgactggc
    11041 gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat acttgcttgg
    11101 gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata aggaaattct
    11161 tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg tccgatttcc
    11221 cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg gaaattgtag
    11281 tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg gtgctctcct
    11341 agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa caaacctgat
    11401 ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc atccaattta
    11461 cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc tttttcaaga
    11521 tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg acctgcaaat
    11581 ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc ttcgcgagca
    11641 agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc aaagatttct
    11701 tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt ccctactgct
    11761 tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc aaacagcaat
    11821 gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat attgatggct
    11881 tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc tactgttgga
    11941 ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg gccgtcaaat
    12001 gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat agctggcgag
    12061 cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg gaagccttct
    12121 gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc ttccgtactt
    12181 ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata cattttttct
    12241 tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga ggccacagct
    12301 gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc ttgttttgct
    12361 agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa tacttgtttt
    12421 tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa caaatgaaaa
    12481 ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca tctagcttga
    12541 tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg tgaatcccac
    12601 tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc ggtaacgttg
    12661 aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt ttttcattag
    12721 gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca agtggttgat
    12781 ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt catggcgagc
    12841 gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt tcacctcttt
    12901 cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag tggcaggtca
    12961 tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca agtagttaat
    13021 cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt tgtggtaaac
    13081 tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat attccatgat
    13141 tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg caaaagaagt
    13201 ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg agttcttaaa
    13261 aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg ttggcttagc
    13321 tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa gtcctgaccc
    13381 agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt tgagccattc
    13441 tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc gagaagtacc
    13501 aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga atggtgaaaa
    13561 acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa tcgaccatat
    13621 caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac taaaagaagg
    13681 cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta caagaaagct
    13741 ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc tccactaatc
    13801 gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat tggatacgca
    13861 tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga tgatagaata
    13921 gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt cctgcttcag
    13981 ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc gtatgcagat
    14041 ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa cgtctcataa
    14101 aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca ccgtaaaggg
    14161 gatgttccac aattacatca atgatttcac caccattttc aacgattagt ctcagttctt
    14221 cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca gtattggttt
    14281 cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct gcagctcgta
    14341 gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc gcaaacttgc
    14401 tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt cgccgtgtct
    14461 ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg agtgtttcat
    14521 caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca gcagactaac
    14581 aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt tttcttcttt
    14641 tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag caaccaactc
    14701 tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg ttagttcttc
    14761 ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt cgggaaaaga
    14821 tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga cacccggcgt
    14881 gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt ttgcgtactc
    14941 cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa gctggacatt
    15001 cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa atgtacaatt
    15061 gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa aattgataac
    15121 tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca attgggatcg
    15181 cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa acatacggtc
    15241 ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt tttctttttc
    15301 ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa acaaccgctc
    15361 cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga ttgattacta
    15421 agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga acttgaaaac
    15481 tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc tgctccatga
    15541 ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt cctccgcatg
    15601 cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga cattttcgac
    15661 aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta gcctttgcgt
    15721 gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct agatgtgcta
    15781 tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg acaaattgat
    15841 agaatttatc cggttcattc accattgctt gatgactcag caagtattcc ggctgctcca
    15901 tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt gtggataccc
    15961 ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct gcttcgtcta
    16021 ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac tcgaacaaat
    16081 ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt tgagcaattt
    16141 atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt ataaaagtga
    16201 ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct tgtatcgcct
    16261 ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa aatggaaatc
    16321 tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt acattagccg
    16381 tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat ctaatattag
    16441 ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca aagaaagcgg
    16501 cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag ttcttttttt
    16561 attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga tagatgattc
    16621 acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt attttttcaa
    16681 aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt ttgtataaat
    16741 aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat taaccctgtg
    16801 gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg cacttttttg
    16861 cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc agtgatgcta
    16921 agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg cctttttata
    16981 tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt catctagacc
    17041 gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg gtttcagttg
    17101 ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca aatgcagatg
    17161 aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca cccttcaact
    17221 ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat agttacgccg
    17281 gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa aaattagtca
    17341 tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag atcgacgcga
    17401 tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt accgatcctt
    17461 attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca aaagcaaaaa
    17521 gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc cactatactg
    17581 tgatcgatca gataccggat gtgcagaaac aacaagcaat ggataatttc tcagcaatga
    17641 gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg gaaggtgtaa
    17701 cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca gatggcttcc
    17761 agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac ccggatatcc
    17821 aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa attatggacc
    17881 aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc atcatttctg
    17941 atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct ggacttacct
    18001 tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg attggtgtct
    18061 tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa atcggaaatg
    18121 cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta caagcaatgg
    18181 tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg attgccgctt
    18241 tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt ggggggattt
    18301 ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc catggccaaa
    18361 cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca acaggaaatg
    18421 agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta gctgatttgt
    18481 tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca tttacgatcg
    18541 tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg gcggtagaaa
    18601 agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat tcaaatttgc
    18661 aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat ctaagaaaaa
    18721 gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa ggtgaagttg
    18781 tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt atcaatttac
    18841 tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta gctcctaaat
    18901 ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc aacctgttca
    18961 acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg aaacgagata
    19021 aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg gatcgttata
    19081 tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca attgctcggg
    19141 cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg ttagaccctg
    19201 aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc ttgacaatgc
    19261 ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc atttttatgg
    19321 ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat ccgaaagaag
    19381 aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag ctgattggga
    19441 caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa aggtaaattt
    19501 ctgaaaattg gctctttgtc aataaggact gatgagttgt g
//
LOCUS       DACDHL010000046        18882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDHL010000046 DACDHL010000000
VERSION     DACDHL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.046"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..505
                     /locus_tag="IV228_001696"
     CDS             <1..505
                     /locus_tag="IV228_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8717589.1"
                     /translation="NYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSI
                     EQPNLDRIKTARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLF
                     LKSILEDSFEYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITA
                     RLISKLQ"
     gene            560..1726
                     /locus_tag="IV228_001697"
     CDS             560..1726
                     /locus_tag="IV228_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8717590.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            2143..5370
                     /gene="fss3"
                     /locus_tag="IV228_001698"
     CDS             2143..5370
                     /gene="fss3"
                     /locus_tag="IV228_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8717591.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            5482..5796
                     /locus_tag="IV228_001699"
     CDS             5482..5796
                     /locus_tag="IV228_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8717592.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5809..6183
                     /locus_tag="IV228_001700"
     CDS             5809..6183
                     /locus_tag="IV228_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8717593.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            6193..6537
                     /locus_tag="IV228_001701"
     CDS             6193..6537
                     /locus_tag="IV228_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8717594.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            6606..7622
                     /locus_tag="IV228_001702"
     CDS             6606..7622
                     /locus_tag="IV228_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8717595.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            8216..10090
                     /gene="ltrA"
                     /locus_tag="IV228_001703"
     CDS             8216..10090
                     /gene="ltrA"
                     /locus_tag="IV228_001703"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8717596.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            10216..10572
                     /locus_tag="IV228_001704"
     CDS             10216..10572
                     /locus_tag="IV228_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8717597.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            10617..11759
                     /gene="dcm"
                     /locus_tag="IV228_001705"
     CDS             10617..11759
                     /gene="dcm"
                     /locus_tag="IV228_001705"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8717598.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            11749..12038
                     /locus_tag="IV228_001706"
                     /pseudo
     CDS             11749..12038
                     /locus_tag="IV228_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12294..13478
                     /locus_tag="IV228_001707"
     CDS             12294..13478
                     /locus_tag="IV228_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8717599.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            13493..13588
                     /locus_tag="IV228_001708"
     CDS             13493..13588
                     /locus_tag="IV228_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8717600.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            14358..16268
                     /gene="ltrA"
                     /locus_tag="IV228_001709"
     CDS             14358..16268
                     /gene="ltrA"
                     /locus_tag="IV228_001709"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8717601.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            16372..16596
                     /locus_tag="IV228_001710"
     CDS             16372..16596
                     /locus_tag="IV228_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717602.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            16609..17112
                     /locus_tag="IV228_001711"
     CDS             16609..17112
                     /locus_tag="IV228_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8717603.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            17239..18276
                     /locus_tag="IV228_001712"
     CDS             17239..18276
                     /locus_tag="IV228_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717604.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            18291..>18882
                     /locus_tag="IV228_001713"
     CDS             18291..>18882
                     /locus_tag="IV228_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP8717605.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPP"
BASE COUNT         6572 a         3057 c         3997 g         5256 t
ORIGIN      
        1 taactataca actcttacga actattaccg tggtgagcga aatcctagaa atactgaaac
       61 atggaatatc cttgctgatt atttccatgt tcctgtaagt tatttgatgg gattagacga
      121 aacaaattcc atagaacaac ctaatcttga tagaataaaa actgctcgtt tagaaaaaaa
      181 ggacaaaata gtcaagaata ttgccagtct tgactcagat tattttactg aattatctaa
      241 tctatacttt tccatgactt ctttacttga agtcaaactt caagaagatg actccgactt
      301 tcttttttta aaaagtattc tagaggactc ctttgaatac acgattctat ttgccaaaca
      361 aaagaagttt attaataaag ctttaaactt aaatatcgat gaaagaaaag agttagaatc
      421 tgagttattt ggcaacttaa cccaagtaca ggttaacaat ctaaaaagag agataacagc
      481 tcgattaata tcaaaacttc aataaaacta attattcaat cagccaactc gccaagttat
      541 cagattggag agattagtta tgtcaataca aaagtacaca acaaaaagcg gtgcacgata
      601 tagtgtccgc ttgtatgttc gtaaagatga aaatggaaag caagaatact atgtaaaaaa
      661 agggtttaaa actgaaaaag aagccaagtt acacgaagca agaaaaaagg tcgagattga
      721 agataccggc tatcataagc cttctaatga cttgtttata acagtgtatg aagaatggtt
      781 gccttcttat aggaatcgtg tacaagaaac gacatatcaa cgtacaaaag accttttccg
      841 cttgcatata ctaccaaaat tcgggaataa gtccatttct aagattactc ctgttcactg
      901 tcagaatgca gtcaatgaat gggctttaaa attcaagaat atcaaacagc ttaagtctta
      961 cagctctcaa atatttgagt atgccatttt tgcagagcta ttacaccgaa atcctatggc
     1021 aaatacaaat ctaccaaagc gggaaaagaa agaaaatgaa aattactttt cattagaaga
     1081 actaaaagct ttttgggata ttctcaataa agaagagcct ttaaaacata ttttaatctt
     1141 ccagcttttg attactactg gtatacgtaa aggagagcta gctgctcttc attggtctga
     1201 catttatttt gaagaacagc taatgtatgt tagaaagagc tatgcaacta ttcgaaatga
     1261 agagaagaat gcaagaagaa aaactgtcag gattcaaaaa aatacaaaaa atatatcttc
     1321 cgaacgaata ttaccaattg atgcacaaac tattgaaatg ttaaaaatct ggaaaaaaga
     1381 acaagctcga gagcttttgc agtttggcat aaatacaaat acaaaaaatc aacttatctt
     1441 tacctatgta aatgcagacg gtgacattaa tcaacctttg catgcggact attcaaataa
     1501 tatattgaaa cgtttagaga agaaatataa atttaagcac gttactattc atggattccg
     1561 gcacactcac gctactcttc tactagaagg tggagcaagt atcaaagaaa cacaagatcg
     1621 attgggtcat aaaaatgctg aaactaccct taacacttat tctcatgtta ctgaaaaagc
     1681 tcaacgaaat gcgttagaaa aattcattgc ttacactggt ttataatatt gtcattacct
     1741 ttctcattac cagaacgaaa aaaagacacc ttctcgcaat gagaaagtgt ctgaaatcgt
     1801 tggtataaca acgattaacg ttttgaaaac tgtgaagctt tacgtgcttt cttaagacct
     1861 ggttttttct tcgtatcatt gaataagttt gaaaagcctt gatttataaa ggtttttccg
     1921 taaatatatt ttgttatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta
     1981 atttaaaggc agtatatttc gtattttata ttaaattttt aggctcattc attctcaaat
     2041 ccattctatt tagtcaataa atattttaaa aagactttat aaacaaagat gttcaagcga
     2101 gcatcttatt tttttgtgcc taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa
     2161 aagaaattat gtgtattaac tattttgagt ttggctgttc aatcaattgt aggagcaatt
     2221 gctcctacga ttgcttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta
     2281 gatttagcac atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa
     2341 gtcactgttc cgcattatgc agtgtacaac ggcgttaaac aagatatttt ctgtgttgaa
     2401 ccaggagtcc cgatttacaa tgagttcact cctggatatg agaagaaccc gttaccagat
     2461 atgtccgata aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat
     2521 actcatattg tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca
     2581 ataaaacgat cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aatcaatcaa
     2641 gccattgccg attatcagaa gaaaccaagc ttccataata gtacggctaa aacagtatta
     2701 gggcaatcga ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt
     2761 gtggaaaata ccgcaaacat tgattatcgt gtgaatggca atcagttgat aatcagccca
     2821 aatgccaatt caaaagaaag tggtgtgttg acgcttaaga aatctgctgg taccggaaca
     2881 cctgtcgcct ataaaaaggc tggccaacag actttaatgg ccggggcgat cgataagccg
     2941 aacacttata cggtcaaaat tgatgtggaa atggagggtt ctttgaagat taaaaaagtt
     3001 gataaagaat cgggtgatat tataccagga acggttttcc atttagactt tggaaaaact
     3061 ttacccgcaa aagacgtgac caccgataaa gaaggcattg cgaccttaga tgagattcct
     3121 catggtacaa aggtaaccat tactgaaaaa tcggtgccgg cgccttatac gattgatacc
     3181 acacccatga ctgccacaat taaagcaggt gaaaccattt cagtgacttc aaaaaatacg
     3241 cgagaaaaag gtcaaattat tctggataaa accggaattg aaacagggag tgatctttgg
     3301 aacgataatt actctttagc gggcaatacg tttgccattc gcaaggatag cccaactggt
     3361 gaaatcgtcc aagaaatgac cacggatgaa aaaggtcatg cagaaacacc aaaagagatt
     3421 gccaatgcgt tggaattggg aacctactat gtgactgaaa ccaaggctag tcatggcttt
     3481 gtgaatactt tcaaaccagt aaaagtcgaa ttgaattatg tcaatcaaac agtggctctt
     3541 gttaccagta acgtaaaagg gcaaaaccaa gaaattactg gtgaaaccac tttgacaaaa
     3601 gaagacaaag ataccggtca tgaaactcaa gggaaagctg agtttaaaag agctgaatac
     3661 accttgttta ctgttaagga tggaaaagcg gtcaaatgga ccgagaactt taaaccagaa
     3721 ttgttgaagg gaacgaaagc ttctgatgaa accgtaactt tgactttaga tgaaaagaat
     3781 caagttgccg ttaaacactt agcgattaat gagtattact ggcaagaaac caaagcgcct
     3841 gaaggatata ccttggatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc
     3901 gaaaaaaatg ctgtgattac tcgagatgtt gcggcaaaag aacaagtgat tcgctttggc
     3961 tttgatttct ttaaatttgc tggatcaact actggcactg ccgaaactgg cttcaatgat
     4021 ctaaccttta aagtgtcgcc attggaagga accaatgaaa tcaccggtgc cgaagatgaa
     4081 gcgaccacag cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt
     4141 ccttatgggg attatttact tgaagaagta gaagccccag aaggttttca aaagattacg
     4201 ccattagaaa tccgttctac ttttaaagaa aataaagagg attataccaa gagtgattat
     4261 atctttacta ttaccgaaaa agggcaaaag caaccgatta aaacggtaac ggtcccttat
     4321 gggaatctaa ccaatgagaa tttctcagtc agtttgaacc gtttgatgct ttatgatttg
     4381 cctgaggaag aagatagctt aacttctctt gcgacttgga aagaaggaga taaagaattg
     4441 actactcttg attctaccga gctagtcgat aaattgagct ataatctaca tgaaatcaaa
     4501 gaagactggt atatcgtagc tcaagccatc gatgtagatg ctacaaaagc tgcccaagaa
     4561 aaagataaaa aagccaaacc ggtggcgatt gcggaaacaa ccgcaacttt agcgaataaa
     4621 gagaaaacag gaacttggga gattctgcat aaattaactg tcgaacaagt tttagacaag
     4681 accattgtct tattcaacta tgtgtatgaa agcaaagaag cctatgaagc tggtgatgag
     4741 ccggtagcga aggatgctag cttgaacaat caagcgcaaa ctgttaattg tatggtggaa
     4801 cgtcatgttt ccatccaaac caaagcgcac ttagaagaag gttctcagac ctttactcat
     4861 ggtgacgtgg tagatatgtt tgatgatgta tccattaccc atgatgtact agatggctca
     4921 aaagaagctt ttgaaacaat tctgtacgct ttatttccag atggtacaag taaagaaatt
     4981 tggaaatctg gcaagattga ttacgaagtg aatgacaaag aatttaccaa aaccgtactt
     5041 gcggaaaaag tggatacaag taaatatcca gaaggtacaa gttttacctt caaagaaatt
     5101 aactatgaca aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct
     5161 caaactttaa cgcccaaaaa agttccgaca acaccaagta caccagaaca atctgaaatg
     5221 ccaactgttc caagtggctc tcaagaatct agtcctacag tgaaggcatt ccctcaaacc
     5281 ggggagaaaa cttccaatgt gctactgttc attggcttta gcttgatctt tgcgacggct
     5341 ggctattatt tttggaatcg tcggaactaa ggtgataaga tgaaacatgt aaatagtaga
     5401 aaacaatacg cgcgaccgcc tcctaaaaaa gtggtcgcgc tatttttgta ttcaaaacta
     5461 acaatgaaaa gaggaaaaaa tatggaattg aaatttgttg taccagatat ggtcgagacg
     5521 ttcggaaaac taacatacgg tggtgaaggt gaagttctat cggaagggta tggtcgaaat
     5581 actacaattg ttggtcgaag ttaccatctg ttttcaaata aacaacgggc cgatgatatt
     5641 gaagtggtgg tagctgcgga agccggcgaa aaggattttg accaagatca accactaaaa
     5701 gctgtaaatc cccacttaat tgccaaaggc tatgaaattg agaatcgtgg gtttaccgat
     5761 tacgtgttgt atgttgatga tttagtgaaa gcataggagg aaaagaagat gagattaaca
     5821 gaaggtattg tcgtagattc aggattaacc tttggcaagt tgcgtttttc cgcattacgt
     5881 cgagaagtac gcaagcaaaa tgaggacgga acggttagta acgaggtaaa agaacggacc
     5941 tataatttga aatcctccgc gcaaggtcga atgatccaag tcagtattcc agcaaatgta
     6001 cccttacgcg agtttgctta tgatgcagag gtagagctgg tcaatccaat cgtggatacg
     6061 gtcgccaatt atgtttttcg ggaaggaacc accgttaatt ggtttatcaa ggcagatgat
     6121 ttagtcttga aacgacaact gaatcaagga aaacctacga accaaaatga aggaaagaaa
     6181 taggtgaaga atatggaaat taatgttgaa catattttag attgtctcga tcaatttggc
     6241 aaaggtgagc taacggagga acaactcact aacgcattaa cctatgatga aaaaatgttt
     6301 ctgattatgc atcaagggtt actggaagta ggtaacgatg ccaaagaaga ctttgatgta
     6361 ctggattggt taggggaaga agagtctttc tttatggttg tcgaggtaaa tgaaagttta
     6421 tgtcggcagg cagaatctgt tttggaagaa atcgatgtgg agatgccgga tgccatcgaa
     6481 agatttttaa aacaattagt ggaaacgaaa caacttcccg tagtagtaaa tgactagcat
     6541 aactttttga aattgcccaa tgaaatccta ttgggctttt tcgactggag gtggaaacca
     6601 tcaaaatgta caaaggacat cgcatacgcg caggagatca acacttgatt tatcactttg
     6661 ttcttggctg gctaatcttt ttgttcattg gttggatgag cgtcttttat ttacaagaac
     6721 ttagacaatt tgatatttct aaggtatcat tttccactag tgaaattggc tggtctatga
     6781 aggatgtaat ctgcttgcta ggaagtctcg ctctttcagg aagcatgatg ttactttata
     6841 tacacttttt tcaggatcat tggcgaagtc tttggcatcg acaaaagtta gctcggatga
     6901 ttctagaaaa ccattggtat gaagtaaagc agactcaagg tgaaggtttt ttcaaagatt
     6961 tgaatagtag ttggaccaaa gagagtatca gttacttccc caaaatttat tatcgaatga
     7021 aagatggctt actttctatt cgcgttcaaa tttcactggg aaaatatcag gatcagctat
     7081 tgaagttgga aaataagtta gaaagcggac tgtattgtga gttagtagaa aaagaactca
     7141 aagactccta tgtggaatac accttgttat acgatatgat tgccaatcga attgggatag
     7201 aagaaatagt ggcagaaaat ggggcgttgc gattgatgaa aaatcaagta tgggcgtatg
     7261 attccttgcc gcatatgtta attgctggcg gcacaggtgg tgggaagacc tatttcttac
     7321 tcaccattat tcaagcacta ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa
     7381 atgcggacct cgccgattta ggtacgataa tgcctcacgt atactcccaa aaagaagaaa
     7441 tttctgaatg tgtggaagat ttttacgaac gcatgatagc tcgtagtagg tcaatgaaag
     7501 aaatgcccaa ctacaaaaca ggagagaatt atgcgtatct cggacttccg cccaactttt
     7561 taatctttga tgaatacgtg gcttatatgg gtgcgaatcg ttttcctaca agcatcgaat
     7621 agatgtgaaa tggaagccta ttgagattat caatagtgac aactaatgtt ttgagagatg
     7681 gaatgaaagc cttcaagttg agctgaaaca tccccgctaa tactcctaca tgctctaatc
     7741 aatgaagtag attggttttg gtatgaagct aggtgaagtc gtctgaaatc tgcctaagtg
     7801 cttgagcata aggctgacta tagggcgggc ggctgaaaaa gataactatg gtaagaatgt
     7861 ctgaaaatga ctgacgaatc tgcgaatgta cgggtctaaa aggctgatct tattgaaaga
     7921 taggaacata cggacgtatg gtgagatacc gacaagtaag gttccatgat atgaaaatcg
     7981 ggatagctaa cgaagaagct ataatttcac aggctcatag tagataccta aggttataca
     8041 tgatagctaa aaacattgga acgatgtaag cagaaaacta tcaattaaat gactacttct
     8101 agataggtgc atataagtaa taacgaaatt gctttgtttc tgtgaaagta agggcacagt
     8161 accgatgaag cgtctaacaa atgtggaggg atagccctaa gtctatttaa taacaatgaa
     8221 tgttgaaaca gatgaagttc aaggatctgg taagaacgtg ggaaaatccg ttatggaggt
     8281 gccacgcttg aacacaaaaa tacgttactg ggaatattat gatctacaag aaacattcga
     8341 tcagctattt actcaaagta gaaacgggaa aaagttttat caattatatg agttaatcat
     8401 ttctgaaaat aatattcttt tagcataccg tactatcaag gcaaacaagg ggtcttcgac
     8461 accaggaacg gattccttca caattgataa ctataaggaa atgaaccaag cagagtttat
     8521 ccatcttatt ctcagtcatt tagaaaatta caaaccaaaa tccataaagc gagtaatgat
     8581 acctaaaccc aatggtgaga aacgcccatt aggaattcca tgcatgattg atcgaatgat
     8641 ccaacagatg ttcaagcaga tactggagcc aatatgtgaa gctaagtttt atgaacatag
     8701 ctacggtttc agaccattga gaagtgcaaa gcatgctcta ggtcgcatta tgtatctcat
     8761 aaacatcagt aaaatgcact atgcagtaga cattgatatc aaaggtttct ttgataatgt
     8821 aaaccaccgt ttactgataa aacaattatg gaatatagga atttgtgaca aacaagtctt
     8881 agctattctt tcaaaatctc tgaaatcacc cattcaagga gaaggtatcc cgagcaaagg
     8941 aacgatccaa ggaggtatca tttctccact attatctaat gttgttctaa acgacttaga
     9001 ccattgggtc tcaaagcagt ggcatacctt tgaaacaaag tatccctata ctaaaggtta
     9061 caataaattt cgtgctctaa gagatacaaa tctaaagcaa ggctatattg tcaggtacgc
     9121 agatgacttt aaaatcatga ctaatgatta tccgacagca ttaaaatggt tccacgctgt
     9181 caagctttac ctaaaagaca gacttaaatt agatatttcc aatgagaagt ctaaaatcgt
     9241 caatttgcgt aaatgtaagt cggaattttt aggattcgct atttgcgtga aacaaaaagg
     9301 gaaaaaatgg gtttgtaatt cacgtatttc taacaaaaaa aaggatcaga ttaaagaaga
     9361 gattaaacaa agaatcaaag atattcaaaa gagtcctaca gcacaaaatg ctctcctgtt
     9421 taatgctctc gtgctcggtg ttcataatta tttcaggtat gccactcata ttaatttaga
     9481 tttgaatagg atgaactacg ttctttcatt cactacttac aaccgattat caaattgtag
     9541 tacaagatgt taccccaaca aagcttcccc aacttacaaa aagttttact caacaacgat
     9601 gaaaacgtat aaagtagctg gagtctattt gtaccctctt tgcgacgtat ctacaaaaac
     9661 aatttacgga tttaatacgg aggatacacc gtttactccg tttggtcgtc aaagattgga
     9721 gcacaaaagc ctacagtctc ttgtgttcca agaattaaga aaattgatgg aatcgaaaat
     9781 accgaataga acggttgagt atttagatag tagaatatct cgttactcaa tgaaatcagg
     9841 caagtgtgag attacaaaac aatttttgcc agcaaaagcc gttcactgtc atcactattt
     9901 gccaaaaagt cttggtggag acgacaaatt cgataacttg agaattattc ataaggatat
     9961 ccatcttctg atccatacaa caaataaaat gataatcgat cactacgtgc atgaactgaa
    10021 actggtgcca gagcaaatag caaaaatcaa cctttatcga aagatgtgta atctgcaaaa
    10081 cattcaatag aaaataaata gatggaacgc cgtatgaggt gaaagtctca cgtacggtgt
    10141 ggagcggggg aaaagatgga gattatatca aattcttacc tatcgctata gatgttaacg
    10201 acaaaagaaa gtgcggtgat tttgaataag cttaaacaaa tcgtgatgtt aggccgtcaa
    10261 tctggtttct ttctgattct agcttgtcaa aggccggatg cgaaatatct aggagatggg
    10321 attcgcgatc agtttaactt ccgtgtggct ttgggtcgta tgagtgagct tgggtattcc
    10381 atgatgtttg gagaagtcga taaaaatttt tttatgaaac gcatcaaagg acgaggttat
    10441 gtagatacag ggggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat
    10501 gatttcttaa gggaaattag caacattgtt ggattaaccg ttcatacgga aagagaaaat
    10561 aatagtgtgt agcctaggaa tatcaataat cctgggcttt tttattgagg tgatttatga
    10621 catttttaga tttattcgcc gggattggtg gctttcgctt aggtatggaa caagccggtc
    10681 accagtgtat cggtttttgt gaaattgatg aatttgctcg gaggagttat aaagcaatcc
    10741 atgatacaag gaaggaggtg gaaatgcatg acatcacaag tgtatcagac gaatttgttc
    10801 gatccctcgg accagtggac atcctttgcg gcggctttcc gtgccaagct ttttcaattg
    10861 cgggaaagcg gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt
    10921 tcgccactct tctccaacct aagtttctat tccttgagaa cgtccgggga ttgctcaatc
    10981 acgaaggagg ggctacgttc gagacgatcc tccgaacgtt ggatggattg gggtatgatg
    11041 tggaatggca agtgcttaac tcaaaggcct acgttcccca aagccgtgag cggatcttcc
    11101 ttatcggaca ttctcgagat gcatgtaccg aacaagtatt tcctatcatt ggatcgtctt
    11161 caacatctga tcaaaacatc agaaacttgg taaatatcaa tccttccaat cgaggaatgg
    11221 gtggccaagt ctatggttca gatagcgtag caccaacctt gaccggtgat gaaggaatta
    11281 aaattgcctt accggtaaag gatgggattt ctgtagcggg tatgttgtcg ggaaattttg
    11341 aacaaggcaa taggatttat gaggtaaccg gaacagcccc cactttgtca acgaaacaag
    11401 ggggaacgaa gattatgatt cggaaaaatg aaacagaata tcaagaggtg aagccagggg
    11461 acagtgtgaa cctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaaa
    11521 gtgtgcatac cttactaaca ggagaccaac aagcggtagt cacggatcag taccagattc
    11581 gaaagttaac acctagggag tgctggcggc ttcagggctt tccagattgg gcttttgatc
    11641 gagcttctca agtcaattca gatagtcagt tgtataaaca ggccggaaac tcagtaacag
    11701 ttccggtcat ttttgatatt gctagaagat taaagggggt aaaagcatat gatttatgac
    11761 gagttgattg gagaaatcta ctggatgatt ggcaagattc aggcagatcc gaaattggaa
    11821 gaagaactgt accggctcaa ttttgatatt cgcaaaaacg gggtgaaagt ccaggtgatt
    11881 cgtatgtagt ggatgaggaa acggatgctc gaatggagct caatcaagtc atcggtgaat
    11941 tggaacgaat tgcggacctt gccaaagaac ctgatatccg ccaatacttg tttgagataa
    12001 aagcagagtt ggaaattgag ggtatcaccg cggagtaata gggaaataac ccactgatag
    12061 gcactgaccg cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgttcatgg
    12121 acggcggcag ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac
    12181 aaaaaacaag caagtaacca taaaaaatgc ttgttgtatc aaggatatta agtaatttgt
    12241 ataggtgtta actcatttgg aaaagttaac accttgaatt tggaggtgaa aacttggcgc
    12301 aacgaaattt agattatcgg ttattaaaag aacgacgcaa cgaatatggc gtttcacaaa
    12361 ataaattagc tactgcttgc ggacttagcc gcccatacct aaatcagata gaaaacggtg
    12421 gcgtaatcgc ctctacaaag accatgagaa aaatttttga tcaactggaa agtttcaatc
    12481 ccgatttacc tttaacgtta ctatttgact atgtaaggat tcgctttccc acaacggatg
    12541 tgcggaaaat tattcaagaa attctccatt tgaaattcga gtatatgctt catgaagatt
    12601 acgcctttta ctcctatcaa gaacaatatg tcatgggcga tattgtagtg atgttatctc
    12661 atgaagaaga taaaggtgtc cttttggaat taaaaggtcg tggttgccgg cagtttgaaa
    12721 cttttttact cgcccaaaag cgtagctggt acgacttttt tgaagattgt ctaaaagccg
    12781 gtggcgtgat gaaacgcttg gatttagcaa tcaatgatcg agtggggcta ctggatattc
    12841 ctgatttaac gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa
    12901 gttaccgttc cggggaactg ttgaaagctg atgaaaagga tggtatgggc aataccttat
    12961 atattggcag cctcaaaagc gaggtttatt tttgtttgta cgaaaaagat tatgaacaat
    13021 atatcaagtt agggattcca ttggaccaga ccgaaacgaa aaatcgtttt gagattcggt
    13081 tgaaaaatga ccgtgcttat tatgcgattc aagatttgtt aaaaggtcgt agcattgaaa
    13141 gtaccacgtt ttccattatc aatcggtatt tgcgatttgc ggataaagta gatggaaaac
    13201 ggcgaaccaa ttggcccttg aatgaacaat ggggtcgttt tattggacgg aatcggaaag
    13261 agattcaatt aacttctgaa ccgaaaccgt ataccattga acgaacatta aattggctag
    13321 gtagacaagt cgcccctacg tggaaaatgg ctaaggagtt ggatcgattg aatcaaacaa
    13381 cgtatatcca ggatatggta cagaatgcgc gactctcgga ccggcataaa aagattttgg
    13441 agcaacaaag tatggcaatc gaaaacttga ttgtatgaaa agaggaattt tgatggaaat
    13501 tgtaaaagat atgttattgg tacttggtgg cagtatgctt ggtgttaccg tgatgtgctt
    13561 gatgaatgca agtgcagcag cggattgagc aatggaaaag aaaggaaagt gacaaaatga
    13621 atttcggaca aaatttatgt gcgactcgtt ccttagtgaa aagctaaggc actaataaaa
    13681 gaacgaaaaa gtttggttat taggagaact gataatctga caggtggaat gatgaagtaa
    13741 cgctctgaaa cgcctgccct gagcctccga caagcattgt atgaggaaac tcagaatgtt
    13801 tgaagctcgg taaagacggc tgaaagatac cgtaatagtt gatgaacata tatcttctat
    13861 cgaaaactat ccagaggtgg gtagcgtatc caacaggtcg ggggtctata aaatatctat
    13921 ggttagaatg ttctataagg aactgacgaa acagcgaatg tacgggtcta aatttgaact
    13981 ttgtagaaat gcaaaggtac gagaaatcgt agctggtgtg gcaaagtaag aatataggtt
    14041 atgaaatgcc atataacgtt acaggcggta tcaagccagc aggcttatag ctagtgacct
    14101 aaggatatat atgtatagat aagattatcg gaacgaggaa aggtattggg ttctcatcaa
    14161 gagaataaac tgacgaagaa caataagcag ttgaactaat gctgaaaagc agagggcgaa
    14221 cctaagaaat tcttgtaatg agaatggagg aatgccctca agtcgtgtat atattagaaa
    14281 agtattatgt tgtttcaata aggattacga gtaagataaa aaagctcact caatttcaag
    14341 taaaggagtg aagccttatg cctaaaaata aaaatgaaac attgtgtgta gaagatttaa
    14401 gacacgctga atattacgaa atgcaaaaca cctttgatga tttatatgcc aaaagtaaga
    14461 atggagatat tttcactcat cttatggata ttatcctatc gagagaaaat atattactgg
    14521 catatcgaaa tatcaaagca aatgcaggca gtaaaacagc aggaacagac ggtacaataa
    14581 ttaaagatat tggcaaattg ccagcagaaa cagttgtcaa aaaagtaaga tacattgttg
    14641 caggcagtcc acatgggtat cgacctaagc cagtgaggcg aaaagaaata ccaaaaccaa
    14701 atggtaaaac cagacctcta ggtattcctt gtatgtggga caggcttata caacaatgta
    14761 taaaacaagt attagaacca atctgcgaag ctaaatttag tgaaaacagc tatggtttta
    14821 gacctaatag gtcagttgaa aatgcaataa aagcaaccta taaccgccta caaatcagcc
    14881 agctacatta tgtgattgaa tttgatatta agggtttctt tgacaacgtg aaccattcca
    14941 agctgataaa acagatatgg gctatgggaa tacgggacaa gcacttgata ttcattttaa
    15001 aaaggatatt aaaagctcca atcaaaatga ctaacggtac aataacatat ccagagaaag
    15061 gaactccgca agggggaata atatccccac tactagcaaa cattgttctt aatgagttag
    15121 accattgggt agaaagccaa tggcaggaaa atcctgttac aaaaaactat gtggttcata
    15181 tcaataaaag cggtagccca tgtaaaagca acgcttacaa agaaatgaag aaaacaaagt
    15241 taaaggaaat gtatatggtt cgttatgcag atgattttag agttttctgc cgctacaagg
    15301 aaagtgctga aaaagccaaa atagcaatca ctcaatggat tgaacaacga ttaaaattgg
    15361 aagtatcaca agaaaaaaca agaattgtaa atgttagaaa aagatactcc gactttcttg
    15421 gtttcaagat taaaatgata ccgagaagaa agaagttagt tgttaaatca catataagtg
    15481 ataaacaatt caaaaaccaa aaagacaagc tggtatcaca ggcaaagaaa attgcttctc
    15541 cctcaaaagg gaaaacggaa ttagaagaaa caagacttta taattcaatg gtattaggta
    15601 tgcaaaatta ttatcagatt gcaacgaatg taaatctgga ttgtatgaaa ctgaatagag
    15661 ctgtaatggc tatcttcact aacagattag gtacaaacaa aaggggcaga ttgagaaaaa
    15721 caggcagaca acttacgaca aaggaggcaa caagatatgg taaatctaaa atgcttcggt
    15781 atgttgctgg cgagggcgaa cctattttcc ctgttggata tatccaacac agaaaaccaa
    15841 tggcaaaggt gtatttagcg aattgctaca ctctagaggg aagaaaattt atccacacca
    15901 atctaagtgt tgataaatta ctgatgtacc agttaatgaa attatctttg gaaaatcgca
    15961 caatagaata cgcagacaat cgaatttcgc tattttctgc tcaacatgga aaatgtgcta
    16021 taactctgga agaatttcaa caagctaatg aaatacattg tcatcatatt gtaccaactg
    16081 gagctggtgg aaatgacgat tataaaaatc ttattttggt aaaagaggcg gtacaccgtc
    16141 taatacacgc taaaacagaa gaaacaatac aaaaatatat agctctattg aagctaaata
    16201 aagtccagct cataagacta aataaatata gagtattagc tggcaatcaa gaattaaact
    16261 tgatataggt ctactacata aatacttttc aatataaaca cgatggaacg cggagtgcgg
    16321 tgaaagtcgc acgctccgtg tgaatggggg gaaaagtttg agatatttat aatatcagga
    16381 acttacctat ccatatatca atggttttta accaatgctc aatctttagt cttactagcg
    16441 attgtggtca ttggtttgtt tttaggattt aaacgggagt tttccaaact gattggcttc
    16501 ttggtgattg ctttgattgc ggtaggtctg gtctttaatg cttctggtgt gaaggatgtc
    16561 ctactcaatt tgtttaaccg aattgtcggt gcctgagggg gaatcacaat ggaacagatg
    16621 cgtgtttata ttgcgaacct tggaaaatac aatgaaggtg agttggtggg ggcatggttt
    16681 accccacctg tcgattttga tgaagtgaaa gaacagattg gattaaatga cgaatatgaa
    16741 gagtacgcga ttcatgatta tgaactgcct tttgagattg atgagtatac gccaattgaa
    16801 gagattaatc ggttgtgtaa cttagcggag gaactagaag gaacgccaat tggtgaagct
    16861 gcttctgaga ttcaacacgc tttcttcaat tctttcgagg aaatggtaga gcatgtggat
    16921 gatattgtct attatccaga ttgcgatgat atgagtgatg tggcgcatta cttgattgta
    16981 gaagctggtg attttggcga agttccagaa caattgaagc cgtatattga ttttgatgct
    17041 tgcggtcgag atttggagat tggaggaaat tatttggtta ctagtcgtgg agtttttgag
    17101 tatccagaat gaatcaaaca ctatatatag tgtttgatta gtatatgtaa gcaaaaaaac
    17161 acaatatata gagtgcaaac tgaagggtaa tgtgatataa tatttgccga agaaatattt
    17221 ttgaaatgag gtaatattat ggcaaatatt caaacaagtt ttattgattt ccataattca
    17281 attagattgg acgtagaaga caatacgcta cttaaggact ataaagacca agtaattgat
    17341 ggattaaaag attacttacc tgatgatgtg aagtttgaaa cttttttaca gggaagctat
    17401 tctgtttata ccggaataaa aagctgtgat gaaaaaattg attttgacat cgatattgca
    17461 gttgctttcg aaattgatca tactgtttat gaagatccaa gggagcctaa actgtgggta
    17521 aaagaggcgc tagttgaaat ttttcctaat gctcaagtta atttaaaagt accttgtgta
    17581 actgctacat ttactggaaa aaagacaaaa aaaaatgtgc acgttgatgt tgcggtttat
    17641 gctaaagaag atgaaaatta tttcttagca aaagcaaaag aattcagtgc tcccgaaaac
    17701 cgatgctggg aggaagcgga tcccaaagta ttaaaagaga aaattaatag tcatgtagca
    17761 gattcagatg atcgaaaaca atttcgaaga tgtattcgct atttaaaaag atggaaagat
    17821 aacaatttta accaagaata taaaccaaca ggaattggac ttacaattaa tgtgatggat
    17881 acgttcctag ttaataagtc cactgatttt ttaactagaa aagttcagta taatgatatg
    17941 gaatgtatga agcagattgt ttcttcgctg aaagattcat ttgtgtatga gtacagtgaa
    18001 acagatggtt ggcattatcg tctacatgca tatcttccag tgaagcctaa cagtgacacc
    18061 tattcaaaaa tgacagtgaa tcagatgagt gattttaaaa acaaactttc taaattatat
    18121 gatgatttga ttttcgctat cgatactgaa gatgagtatg aagctacaaa acgattaaat
    18181 aatcaatttg gggaggattt tttaattatt tcagaagaag aagttacaga aaaaaactta
    18241 agaaatgcat ttgttacaga ctatccgagt gcttaggttg gtgttaaaga ttggattata
    18301 ctgatatttt aaaagaaaag ctaacagatg ttgtgcgttt attcgatggt aattactatg
    18361 gggatttttc atttgctaca attggaaagt tgcgtttaaa aattgaaata ccaaaacatt
    18421 ttccattggt acttcctagg atttacattc ataattttga tcatttagga ctttacctac
    18481 cacatgtaga aaaagatggg ttggtatgtt tttcttcaga tataaattat ttagtagatg
    18541 aagaaaattc agaattggtt ttattgcagt gtgttaataa aactataaaa acaattgagg
    18601 aaggtttgaa agggttaaat aaaaatgatt ttcgaaaaga gtttgttgct ttttgggaca
    18661 aacaagagaa tcaaatttcg ttattctacc tttgtgaacc agcgttgttt atcaagtcag
    18721 ttgatacaat aatgtacggt aagtctttgg ttgctttgga taaagagaac gaattacaat
    18781 taaatgcagc gagtagattg tttaatggag agaaagaaga tactcatgaa ttagatgcat
    18841 actatgttcc aattagaaac aagaataata ttatgccccc ga
//
LOCUS       DACDHL010000047        18800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDHL010000047 DACDHL010000000
VERSION     DACDHL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.047"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..257
                     /locus_tag="IV228_001714"
     CDS             3..257
                     /locus_tag="IV228_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8717606.1"
                     /translation="MEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQLIGFGNF
                     EVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            492..1538
                     /locus_tag="IV228_001715"
     CDS             492..1538
                     /locus_tag="IV228_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717607.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            1540..2799
                     /locus_tag="IV228_001716"
     CDS             1540..2799
                     /locus_tag="IV228_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8717608.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            2805..3386
                     /locus_tag="IV228_001717"
     CDS             2805..3386
                     /locus_tag="IV228_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8717609.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3468..4343)
                     /locus_tag="IV228_001718"
     CDS             complement(3468..4343)
                     /locus_tag="IV228_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8717610.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            4491..4823
                     /locus_tag="IV228_001719"
     CDS             4491..4823
                     /locus_tag="IV228_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8717611.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            4935..6143
                     /locus_tag="IV228_001720"
     CDS             4935..6143
                     /locus_tag="IV228_001720"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8717612.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            6297..6752
                     /locus_tag="IV228_001721"
     CDS             6297..6752
                     /locus_tag="IV228_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717613.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            7114..7389
                     /locus_tag="IV228_001722"
     CDS             7114..7389
                     /locus_tag="IV228_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717614.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            7402..9480
                     /locus_tag="IV228_001723"
     CDS             7402..9480
                     /locus_tag="IV228_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8717615.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            9640..11526
                     /locus_tag="IV228_001724"
     CDS             9640..11526
                     /locus_tag="IV228_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8717616.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            11538..12485
                     /locus_tag="IV228_001725"
     CDS             11538..12485
                     /locus_tag="IV228_001725"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8717617.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            12505..13032
                     /locus_tag="IV228_001726"
     CDS             12505..13032
                     /locus_tag="IV228_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8717618.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(13095..13748)
                     /locus_tag="IV228_001727"
     CDS             complement(13095..13748)
                     /locus_tag="IV228_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8717619.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            13880..14722
                     /locus_tag="IV228_001728"
     CDS             13880..14722
                     /locus_tag="IV228_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8717620.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            14880..15776
                     /locus_tag="IV228_001729"
     CDS             14880..15776
                     /locus_tag="IV228_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8717621.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            15779..16492
                     /locus_tag="IV228_001730"
     CDS             15779..16492
                     /locus_tag="IV228_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8717622.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            16512..17030
                     /gene="msrA"
                     /locus_tag="IV228_001731"
     CDS             16512..17030
                     /gene="msrA"
                     /locus_tag="IV228_001731"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8717623.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            17027..17254
                     /locus_tag="IV228_001732"
     CDS             17027..17254
                     /locus_tag="IV228_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8717624.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            17406..17957
                     /gene="lepB"
                     /locus_tag="IV228_001733"
     CDS             17406..17957
                     /gene="lepB"
                     /locus_tag="IV228_001733"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8717625.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(18040..18582)
                     /locus_tag="IV228_001734"
     CDS             complement(18040..18582)
                     /locus_tag="IV228_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8717626.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
BASE COUNT         6535 a         3194 c         3845 g         5226 t
ORIGIN      
        1 taatcgaaaa agttgcttca gctactgatt taactaaaaa agatgcaact gcagcagtag
       61 atgctgtatt ttcaactatt caagatgctt tagctaacgg cgaaaaagtt caattaatcg
      121 gttttggtaa ctttgaagtt cgcgaacgcg cagctcgcaa aggtcgtaac ccacaaactg
      181 gtgaagaaat cgaaattcca gctagcaaag tacctgcatt caaaccaggt aaagcattaa
      241 aagacgctgt taaataagac agttgaaaaa gcgcttgtgg ctctagggct acaagtgctt
      301 ttctttttat ttcctagcag aaaaggaata aataagagac agagattccg aatccgtatg
      361 tcatacatag aaaacacatg tattttattt aatttttatt tatttgcaag ttatgaggtg
      421 agctttgctc ttatcttttc tagaatttgc ttactaatgg taaaataaaa ataagaagag
      481 gagggatgcg tatggaactt gattatcaag tagaagatgc gttgacgaaa gtgttttacg
      541 gagaaacgct agtcagtcag ctaagagaac tttccattga tcagccaatc ttatttttaa
      601 cgaatcagcg ctattatgat ttgtttgcag ataagatcaa cccgttattt gcaaatcaag
      661 caaacaatga ctggtatatt tgtgccaaca ctcaatgcaa tcatttaaca gaattaaaga
      721 acctgcttga tttcgtcaaa cgctatcccg aaaatcaatc catgtttatc atcggatttg
      781 gaaatgaagg gatcatggat ctggcaggtt tttttcaaaa acatacaacc cttgattcat
      841 cactatggat gatccctgta tcgatccgtt ctctaagccg agcattaaca ccgcagcgga
      901 cgattttgca gctaccaaat cgtgtagtgc tgcaaaccat caatctgcca gaacgcatcg
      961 tttatgatca aacaatcagc gagcggcagg tggacggaaa gcagattgac tttttgactt
     1021 ttatctgttg cggaatgatt tgtgatcatc gttttttgca aaatctgtat aaaaactatc
     1081 caaataaaaa gcaattaacg actcggccgt ttgccgggat gatcgaagaa atgatctatt
     1141 tttatcaatt agaggcagaa aacctgtcaa attatggtaa actgtttgaa caagcttttt
     1201 atctgaccga aaatggccat ctactttctg ctagtatgaa aaaattttta ggaatgatgt
     1261 ttcatttcgt gtggaatctc caagcatatc cttttgattt ccaactaaaa aacttttttg
     1321 tgtggctgga cttgcttggc tatccaatcc actggctgga acagatttca aagatggagt
     1381 atttggaaaa agttcttttt ttggcagaaa aaagcaaaaa aatacttgtt ttagaaaaaa
     1441 taggtataat agacggatac caacatccaa acgaaaaaga attattgaga acaatgacgg
     1501 tatttcaaca aatcgtcagt gagataagag ggaattgaaa tgaatacaaa tagcgaaaaa
     1561 atgttgcaag ctttatcaga tgaagacttg tcacaagcac aaatcttttt agaaaaagca
     1621 ttaaaagaag acccagcaga tatattagct gaactaggcg aagaattgct ggctattggc
     1681 tttttgatcg aagcaaaaca gatttttgaa caactgatca accaataccc agaaaatgac
     1741 ggactgaaca tcccgttagc agaaattgca atcgaagata atgagatcga cttagctttt
     1801 gaatacctgg aaaaagtgga taagcaaagt gattactatc cgcaaagctt attggcgata
     1861 gctgatttgt accaagttat cggaatccct gaagtgagtg aagcaaaatt gaaagaagca
     1921 gctgggttgc ttcctgatga accattgatc cagtttgcgc tagctgaatt gtatttttca
     1981 gttgatcgtt tcaaagaggc agcagtggtt tatgaaacat tgttggcaag cgggataaat
     2041 gaaatttcag gaatctcgat gcaggaacgt ttaggacaat ccttaagtat gcaaggagaa
     2101 ttcgaagcag ctatcgtacc tttagaagaa gcactgaaag aagaaaggac agatgatcga
     2161 ctgttccagc tagcttttac gaatcttcaa ttgaaagaaa atcagcaggc aatcaactat
     2221 ctggaagaac ttcgagaagt taatccgcat tatcaatcgt tatatctata tctaggacaa
     2281 gctcttcaag aagaagaaat gattgaagaa gcacaaacag tgttagaaga aggaatcaaa
     2341 gaaaatcctt accaagtcga actttatcac ctggcctcgg aaaatgcttt ccgtctgcat
     2401 gatagacagc gagcagaaga acttttactg aaagctttag aagtaggaga caaacaagat
     2461 gaaacattac ttactttaag taatctttac ttggatgatg aaagatatga agatgtgata
     2521 caagtgatca atcagatgga agaaactgct aatccttatg cagaatggaa cctagcccat
     2581 gcttataatg aattagaaga ctttaccgtt gcagctgtac actatgaaca ggcttatcat
     2641 gaactatcac atgaaccaga tttcttgaaa gagtatgcct ttttcttaag agaagaaggg
     2701 cagctgaaaa gagcgcaaga attattgact tattatttaa cacttgaacc aggagatatg
     2761 gaggcactgt cattattaga tgacctaaca gagaggtgag aaaaatgcta attgatgtac
     2821 gggaaaagaa gaatttttta acgtggatgg tcaatcatgt atcttttagc cgaagagaag
     2881 ttttttggat cttgaattat cttgccaatc atgaagcgat tttaagcaat gtccactttg
     2941 ttgaaggggc gaattttgcg gatcgaggac tgcagatcag tgatctatct gttgaagggg
     3001 aacccatgaa attgtttttg cagggaaaag aatttacgga cacagaccag atttttcatg
     3061 aaatccgttt gaattggaaa aagcctttat atgtggaatg tatattcgaa aatgcttggc
     3121 agacaagaga atatctatct atcttagaag acaatccatt ttcttcttgg gattcttcag
     3181 tcagtgaaga agatactgaa ttaattgaac gatattttca agaaaaagaa gaagaggcaa
     3241 aactaaaaat gctttatgct caaatcgatc aagcactaga agatggagat agggatgctt
     3301 ttttgaaatt atctgatgaa gttaaccgaa agatcctttt gcagacacaa aagaaaagta
     3361 accgggtaag tgaacataac aattagttaa aaatagtata gtactaaaaa agacaaaggt
     3421 cagctggatg tggaatcttt cctcatagcc tatctttgtc ttttttctta tagttttttc
     3481 agtaatcttg tctgtggctt caaataagta aagccttgcc cttccacttc atgcacattg
     3541 atgattgaaa ggaaagcttt ttcgtcaatc tcgtggacga tcctcttagc ttcagttaat
     3601 tcactagagc taacgaccat atagatgatt ttttttgaat taccagagaa tgcgccttct
     3661 ccagaaagga aagtcacacc tctttgcaga ccggtcatca gtagtggtgc aatctcttcg
     3721 cttttgttgg agacgacaag tagacctttt gcagaatatc cgccatctaa gatcgaatca
     3781 atcacacgac tgaaaacata cgaaacaatc aacgtataca tcatacgttt caagtcgata
     3841 taggttaatg aagcaagtaa gacgatgata tcaaatgcca atagggaacg ccccatagtg
     3901 atgccgtaat tttgttctaa aatccgagcg ataatatcac tgccgcctgt tgttccaccc
     3961 atgcggtaga cgatcccgct tcctacacct gcaactaaac ccgcaagtag agaaacgatc
     4021 agtagatcat gctgtaaatt gatttctagt ggaacccttt gccagatcca taaaaagaat
     4081 gacaaagaaa gtgtacctag aacagtatag gccaaggaac gtttaccgag gatctttcct
     4141 cctagtaaaa tcaatgggat gttgattaat aaagtcgtat aagctggatc aataccaaat
     4201 aaagctctta agatcaaggt gatccctgag actccccctt ctgctaaatg attagcgatg
     4261 ttcaagtaaa caagaccaaa tgcataaata cttgttccta taatgataaa aagaacttct
     4321 tttactgttt ttcgtttgac catatgaatt ccagctttca attatgtatt ttcacagaat
     4381 ttccatttag aaattccttc ctaaatgtag catggaaatt cgattttctc aattgctctt
     4441 atctaggatt tgcgttaaag ttttggtaag atagttttgg agggaaaaag atggataaaa
     4501 agcgttcttt atacagtatg cagcaggaag tggatgaata tatccagcaa ttcaaggtgg
     4561 ggtatttctc tccattagct cagatggctc gattaactga agaagtaggt gaattggcaa
     4621 gagaagtcaa ccacacttat ggagaaaaaa gcaaaaaggc ttctgaacca gttaactcgg
     4681 tagcagaaga acttggtgat gttttattcg ttacgatgat catggctaat tcattgaata
     4741 tcgacttaac tgaagttttt gaaaaaaaca tggaaaaatt caatcaaaga gaccataatc
     4801 gctttgagcg taaggacgag taaacaagat gaagtggtcc tggccttgaa gactctgaaa
     4861 cggacttgtc aaacagtcac catgcaactg agtctttatc tcaggacaag aaacacgcag
     4921 gaaaggttgg aaaaatgaaa ttaaaacaat tacctctgga ataccaaaag gcaattccag
     4981 ttctgaaaaa attagagaat gcaggatatg aagcttattt tgtaggtgga agtgttcgtg
     5041 atatcttgct gaaccagcca atccatgatg tggatatcgc aacaagcgca tttccagaag
     5101 aaatcaaaca gctttttcca aaaacgatag atattgggat cgaacatgga actgtgttgg
     5161 tattagaaaa tgatgaacaa tatgagatca ccacatttcg gacggaatca acttatcagg
     5221 actaccgtag accagatcat gtcgaattcg ttcgttcgtt ggcagaagat ttgaaaagaa
     5281 gagattttac gatcaatgcg tttgctttga aagaagatgg agaaatcatc gatttgttcg
     5341 atggtctgca agatctagaa gaacagatct tgcgagctgt aggcaatcct catgagcggt
     5401 tccatgaaga tgccttacga atgatgcgcg ggttgcgttt tgtcagtcaa ctagggttca
     5461 aattagagga agaaaccttc tacgccatca aagaaaacca cgagctatta ggtaaaatat
     5521 ctgtagaacg tataaatgtg gaattcatca agatgctctt gggaaatgaa cgggccgcgg
     5581 gaatccatgc atttattgaa acagagtgct acatttgctg tcccggtttg aaagaagaag
     5641 gcgaagcact tttacgtttt gctgaattac catccgtccc attgaaagaa gagagccaag
     5701 catgggcatt attgatcagc caactggatc taaaagaaaa tgagattcgt tcctttttga
     5761 aatcgtggaa gtgttctaac caaatgatta aagacgttca agcacttgtc caaggactac
     5821 gacaacgaga aactggagct ttggaaccat tcgaattgta cttcttaggc aaaaaacatg
     5881 cactgcaagt agaagaattg atgatctatt ttgacgaaac acctcagatg gatgccgtag
     5941 gagaatccta tgatcgttta cctatcaagt cattaagcga attagcagta gatggtaaaa
     6001 tcttgctaga agaaactgga aaaaaacctg gtaagtgggt tagtgaagct ttgcaattag
     6061 ctgaaaaagc cgtcgtagaa caaagaataa aaaatgataa aaaacaggtt ttggattttt
     6121 tatcgaaaga aaaattgatt taaaaaacac tagactgtat ctgctcagct ttgttggcta
     6181 cttaaagtgg gcagatacgt cattagaaga aaaaacataa gatgtgacca cttacataaa
     6241 tttgtgctaa actgaggtag tacaagaata aattacatca taaaggagtc tttttaatga
     6301 gtaaagaaat gacagcgcta aaattttatt ttagaaatgg agaaacctgg acgatcaacc
     6361 gccgtcatat cggcgatctt tggatcaaac aaatcacaac aagttttgga cgaatcaatg
     6421 gcagtgaatt tgttgaaatc catccgtgtg ctggatttaa aatcgaaatc ttccatgaag
     6481 gggacgcagt tgcgacacat gatatcaacc taggcggact ggaaatgggc atgtttaatc
     6541 gcgcattgaa atacgaagat atcgagcgaa tggaaatcct ttaccgaaat ggcacacctg
     6601 atcttgtgta cttcccatat ctagataaag gaacagaagg attagacaat caatatcaat
     6661 caacaaaaat cagcgaaaaa acaggtaact tgtatatcgt gatcaatcca gatcaacgtg
     6721 tagaagatgt atacggggaa tttttcgaat aaatcgattt ttacgcaaaa atgaggctgt
     6781 gaaagagatg tgtcttatta ctactgatag gctgtctctt ccacaccgtt tattcagaat
     6841 aagggactgt gacaagattt ttgtcgcagc cccatttttt tgttgtgata aaagaacaag
     6901 aaaggcagga atgggaaagt aaacatcctt ttgtcagaaa cttttatctt tcttaagttt
     6961 tacttgatat ctgatactat ttggaataga atgaggcgtt gaaaaattta tggaaatttc
     7021 taactagcaa taaacgatga aacaatgaaa tatttttcat ccaaaaagaa ctttgctata
     7081 atgatggaag tttgtgtata ggagatctgg aaaatgagag agagttttga acaacaaaaa
     7141 aaactgttgc atgatcgcta tggcgccctt tcgatggatg accgccgtca aatactttgt
     7201 aagttgcgta aaagaaatat attgatgtat cgtcagttgg aacgtctgaa acatgatctg
     7261 cttcgtttgg agtcaaaacg tgtacagtgt gaattagagg ggaaccaaac acaggttgaa
     7321 gtagtcgaaa ccaaaatttt aaagaaaaaa gaacaatttt taaaaatgct tacacaaaat
     7381 aaaaaataaa ggggatggac gatggaattt gctgagttag ttattgtatt tgcagtaacg
     7441 atcaccttat cgaacatagc tagccgcatt ttgccaatga ttccagctcc actgatccaa
     7501 attttccttg gagtggtatt aggtctgaca gagtggggac aatcaatcga ttttgaacct
     7561 gaattgttct tagttatgat tattgcacca ttgttattta gagaaggaga aaaagcagat
     7621 atttcttcga ttctcaaaaa tttcgggaca attttgttct tggcatttgg aggagtcatt
     7681 ttgacccttg tcggggtagg tgcgacgtta tcattcctgt tgccaagtgt cccacttgct
     7741 gcctgctttg cttttggagc agcgttaggt ccgacagatg cggtagcagt cagttctctt
     7801 tctggacgag tgaatatccc taaaaaggcg atgcatattt tggaaggaga aggtcttttg
     7861 aatgatgcat ctggtgttac tgccttccag tttgctcttg gtgcgctgat caccggatct
     7921 ttttcagcag taaacgcggg gatgagtcta gtagtttcta gtattggggg agctttgatc
     7981 ggctttttgc ttgtctggtt caaacaaaaa ataattcatt tgatcgaaaa agcatcagca
     8041 caagatgtca cagggtatct gctgatcgaa cttttgcttc cttttctagc ctatgtattg
     8101 gcagaggcat ttgatgtatc tggaatcatt gctgcagtgg cagcaggtat tctccaagct
     8161 tctggtttta ggaaaatctc tgtgtttgat gcagaattgt cgagcttgtc ccacagtaca
     8221 tggacaacta ttgcttttac actgaatgca ttagtattta tatttttagg aatcgaattg
     8281 acgcaagtgt tctcgccagt atggggagac ggtttatatc caaacggtct tttattagcg
     8341 attatcgtat taatctctgt catgcttttt gtgatcagat tcatttctat cagtcttttt
     8401 tatgtattca aagatggcag taaaaagttc aaaaaacagt tgaacgagat cttgatctta
     8461 acatttggcg gtgtaaaggg aacagtcagt ttggctacga tttttatttt gccattttca
     8521 atcaataata tgatgtttta ccagcggtct ttgcttttgt ttctgacggc gggtgtcatc
     8581 ttagtgactt tggtcatagg gatcattgtc ttgcctatgc tgacggaaac agaggaagcg
     8641 aaaagcacgg atttgaatgc tttgatgatt ttagaagagg tcgttgaagt tttgcgaaaa
     8701 gaaataaagg aaattgatcg taatacaaaa gaatttttag ccactgaagc agtgatagaa
     8761 aattatcaag aacggatacg tgatctgtac ttggaagatt taaccgatga tgaaaagcaa
     8821 gaagtacaag aaatacaagc actgattttg tcgatcgaac gggatggatt ggatgaaagt
     8881 tatcgttcgg gcaagctttc ttctaatggt tatcgttttt actcccgttt tctttctcga
     8941 ttcgaacatt ctatcacaag ccagattttg tcttttatcg gcttttggtt cattgttgtc
     9001 cgtaggttaa tgcgaatcgt tctccatccg aaaatgtttt ggcaaagaag atatgctgat
     9061 cgtcagacat ttattagtga tgaagatatc caagaaatca gagagacata tcaaaaaaat
     9121 actcagttga ttattgaaag tttagataac ttgactgata tttatgatga aacgatggtt
     9181 caatttttta tccagcaacg caaacttgaa ggaataaaaa tgaactcggg gaatttgatt
     9241 tcttcgtgga tgatccagca agatagtctg tttacgaaaa aaatgctaag aggttattat
     9301 cttgaaagaa aaatcattga tgaatacgaa gttgctgaaa aaatcacgac gttttctgcg
     9361 aacagctatc gcaggaatat caatttgtta gaatcttata cgatgaacaa accatctgat
     9421 aacttttctt tccgatttgc gtttcgagca aagtcaaaaa aaacgagtcc aataaaataa
     9481 aaaggtaaaa attttcaagt tttataaaaa acaaaacata tcagaacagg atcgaatcgg
     9541 tcctgttctt tttttgagaa cacaaaaaga acaaatacca aaaaaatttt cctgaagttc
     9601 actattttta tgatacaata tccaagataa ggagggaaca tgttgaaaga attaaaagta
     9661 actgacctca aaaaaactta tggtgaaaaa gatttgtttg accagatttc ttttttggtc
     9721 cacgaaaaag accgtatcgg gttgattggg acgaacggga cgggtaaaac aagtctctta
     9781 aatattattg ccggtatcga tagcagcgac ggtgatcggc aaacagtgtt ttatccaact
     9841 gattaccgta ttggttatct ttctcaagca gctgaatttt ccgatgaatt aactgttctc
     9901 caagcagtct ttcaaggaaa cagccccttg atccaaactg ttcgagcata tgaggaagca
     9961 ttgatcgaac taggggaaaa cggagaagac cctgatgtgc aaaaacgtta tgctaaagca
    10021 gaagaacaaa tgaataaaga agatgcttgg actacagata ccaacgcaaa gattattttg
    10081 caaaaattag gaatcagtga attagataaa aaaatcagta ctttatcagg tggtcagaaa
    10141 aaacgagtta gtctggcgca agtgttgatt gatgaacccg atttactatt actagacgaa
    10201 ccaacaaacc atttagatta tgaagcgatc gagtggttgg aaaactattt gaaacaatac
    10261 cgtggcgcac tattgatggt tacccacgat cgttattttc tagatagagt agcaaaccga
    10321 atcttcgaac tttcatttgg aaaattatac gagtataaag gaaattatga agcttacgtc
    10381 ttagaaaaag cagaacgtga tcgtgtagaa gtagaacaag aagaaaagcg caagcggctt
    10441 tataaacagg aattagcttg gatgagggct ggcgtacagg cacgtggcac caagcagcaa
    10501 gcacggatca accgtttcga agatctaaag gagaacttgt accaagtaaa ccaagaagat
    10561 gatttagagc tgaacctagc gacacaacgt ttagggaaaa aagtactgga aatcaaagat
    10621 ggcagttacc gtataaatga ccaaacactg ctagaacatt tagatttatt gattcaatct
    10681 cgggaaagac taggtattac tggtaaaaat ggtgcgggaa aatcgacatt gcttaatatt
    10741 ctagctggca gaattcccct ggatagcggt acaatgtcta tcggtgaaac ggttcatctt
    10801 gcgtattata cacaagaaaa tgaagaaatg gcaccagata aacggatgat tgcttactta
    10861 caagaagctg ctgaagaagc aaaaacagca gatggcagtc aaatcggagt agcagaatta
    10921 cttgaacgat ttttatttcc aagatttatg catgggacac tgataggcaa actttcaggc
    10981 ggagaaaaac gccggttatt tttgttgaaa ctgctgattc agcaacctaa tgttttatta
    11041 ttggatgaac cgaccaatga tttggacata gcaacattga cgattttgga agattatttt
    11101 agaagttttc caggtgctgt gatcactgtt tcccatgacc gttatttctt ggacaaggta
    11161 gcagataagt tattagtatt ccaaggaaac ggaaaacaag aactttatta cggaaacatg
    11221 tccagttatt tactgaagca aaaagagacc cagcagcctg ctgaaaaagc gaaaccaaaa
    11281 atacagagca aggaacctgc tgaaaagaag aaattatctt acatggaaca aaaagaatgg
    11341 gaaacgattg aagatgagat tgcagaactt gaagaaaaaa tctcattgct ccaagaagag
    11401 atgaatcatc aaggagataa tttcactcga ttacaagaat tgcaaaacga cgtatctgaa
    11461 acagaagcac aacttgaaga aaaaatggca cggtgggaat acttgagtga atgggtagaa
    11521 gattaggagg aaaaaggatg gaacaagcgt atcttgactt aggaagaaaa ttgttggaag
    11581 aagggcatcg taaagaagac agaacaggca caggcacatt aagcttattc ggataccaaa
    11641 tgcggtttga tctgcaaaaa gggtttcctt tgttaacaac gaaacgagta ccttttggat
    11701 tgatcaaaag cgaattgctt tggtttttaa aaggagatac gaatatccgc tatctattac
    11761 aaaataaaaa ccatatttgg gatgaatggg catttgaacg ctacatcaaa agtccagact
    11821 ataatggacc agatatgact gattttggcc atagatgttt gacagatcct gaattcaatg
    11881 aagaatataa aaagcagagc aagctttttt gcgagaagat cctaaccgat gattcctttg
    11941 ctgaaacata cggcgatctt ggacacatct acggctatca atggcgtcac tgggaaacaa
    12001 aagacggtgg ctttatcgat caaatcaaac aagtgatcga agcaatcaaa aagactcctg
    12061 attctagaag attgatcgtt tctgcatgga atccggaaga tgttcctagc atggctttgc
    12121 ctccttgtca tacgatgttt caattttatg ttcaagaggg acggctaagc tgccaattgt
    12181 atcaacgcag tggggatgtg tttctaggcg ttccgttcaa cattgcaagt tatgcgttgc
    12241 tgactcacct aattgcacat gaaacaggtt tagcggttgg cgaatttgtt cacacattag
    12301 gagatgctca tctttatatc aatcatatcg aacaaatgaa agaacaattg agccgagaaa
    12361 tacgcgcatt tccgacttta gttttgaatc aggaaaagca gtcagtattt gattttgaga
    12421 tggaagatat ccaattagaa ggatatgacc cacatccggc gatcaaagct cctatcgctg
    12481 tttagaatca gaaaggatga gaaaatgtta gctgcaattt gggcacagga tgaaaacgga
    12541 ttgattggaa aagaagaccg tcttccttgg cgtttgccaa atgatctgaa attcttcaaa
    12601 caaatgacag aagcaaatac actagtgatg ggacgaaaaa cattcgaagg gatgggtagc
    12661 cgtccgttgc cgaatcgtca gacgattgtt ttgacgagag attcttccta taaagcagaa
    12721 ggcgtgcatg tcatgcatag tgtggaagac gtgcttgctc tggaaaaaga aacagacggt
    12781 atctttttca tcgctggagg gtcagctgtt tatcaagaat ttctgccatt ttgtacgata
    12841 ttataccgta cagtcatcca tcatgtattc gatggagatg cttattttcc acctgttgac
    12901 tggtctgatt ggtcactgat caatactagt caaggagaaa tggatgaaaa aaatcgttat
    12961 ccgcatcaat ttgaaacgta tcagcgtaat gagaatcctg taaaaagcgg gacaggcgaa
    13021 ttgacgatat aaaaaagcta tggatagact agctgttatt tagagctgtc ccatccatag
    13081 ctttattttt tttattaagt aaagaataaa acagagaagt acataaaacc tgcacctaac
    13141 ataacaaaca aatgccaaac aacatgcata tagcggatag aattcaagct ataaaagaat
    13201 gcgcctatcg tgtaagaaag cccgcctgct accatcaaaa taacaccagt cattccaaga
    13261 gagtgataaa gaggaacagc agccacgaca cagagccacc ccataaaaat atagatgatc
    13321 gtagatactt ttgatactct ctcccgttta tgcaacgtca acgctttata tataatgcca
    13381 agaatcgcaa acaaccagat taatacaaat aaagtccaac ctagccatcc tcggatactc
    13441 agcaaacaaa aaggagtata acttcctgca atcaaaagaa aaatcgaact atgatcgaat
    13501 acttggaaca ccttttttgc ttttgtaaaa atcaatgaat gaaacaaagt agacgttaaa
    13561 aataaaagaa tcatcatcga tccatagata gcataagaga caatatggag ggaggaatgc
    13621 aagtgggcac cttttactaa aagaaccact agtcccgcga tactcaatcc agtcccaatt
    13681 ccatgggtga ctgcattaaa cacttcattt aatatttcat acgttcgatt aaatttcgtt
    13741 ttttccatga aaacggctcc tttactaggt gtaattaatg aaaaatcatt atttgtctgt
    13801 tatactaaaa atagaaattg tcttacatgt agtttaacat gaataaggca tcttgttaaa
    13861 gaaagagaga gagaaaataa tgacaaatgt aaaaatcgta acagattctt catgtacgat
    13921 ggaaaaaaat gtacgcgatg ctttaggcat tcgtgtcatg ccgctatcca tcatgatcga
    13981 cggaacagta tatcccgatg atgaccaact agacggcgaa agatttatgg aaatgatggc
    14041 gaaagcaaaa aatctgccca aaacaagcca accaccaatt ggagaattca tctcgatgta
    14101 tgatgagcta ggagaagacg gcagtgaagt gatttcgatc catatgacaa aaggcttgag
    14161 tggaacagta gaagcagcaa gacaagccag taatctaaca aaaacgaaag taacggttat
    14221 cgacagtgat accactgacc aaggattatc ttttcaggta attcgagcag ctgaattagc
    14281 aaaagaaggt aaaacagtag agaaaatcct tgaagaagtc aaaaaagtac aagacaatac
    14341 aaaattatac atcggaatat ccacactaga taacttggta aaaggtggaa gaatcagccg
    14401 aacaactggt ttgctgtcta gtgttttgaa catacgcgtg gtgatgaatt tcgcccattc
    14461 tgaattaatc cctgtaacta aggggcgagg aaagaaaacg ttcgataaat ggtttaataa
    14521 tttgaaaaat gaactgaaag aattgccaaa tgtgcgacaa atcggtattt cacatgcgga
    14581 tgcaaaagaa ttggcgcaag aattcaaaga gggtttgcaa gaattattcc cggacatgca
    14641 tatccctgta ctgcatacga atccggttat cgctacgcat actggtaaag acgcgtttgc
    14701 aatcatgtat tatgtagatt agtcgaaaag aggataagcc ctcagcctga aaggcttgca
    14761 gcttattctc tttttcttta taaaattagg aaaataattg ttttcttttt tcataaagac
    14821 tacctttttg ataaaatttt tgataacatt agaaccaatc gaatacaaga ggtgaaaaaa
    14881 tgaaattaac gaaacgacat ttgatcctcg ttttgttccc attattaatt gcgatcatca
    14941 tctatgcagg tttagctgct tctataccga aagctggccc actaatgaaa ccagtaacta
    15001 ctgtgagaga agatccaaat aaaaaagaaa aacttcacta catagctatc ggagattcgc
    15061 tgactgaagg agtaggagat caaaccaagc aaggaggatt cgttcccctt gtcgcaaatg
    15121 atctaaaaga ccgttataat ttgaccgcgg ttgaaacaga aaactatggt gtaaatggtg
    15181 aacgaagtga ccagatctta aagcgagtaa aaacaaaaga agaaattcga aacaatatca
    15241 aaacagctga ttttatcaca ctgactgttg gcggtaatga tttgatgaaa gttatccaaa
    15301 ataatttatt cgggttaacg gtcaattcct ttaaaaagcc actgaaaaac tatcaggaaa
    15361 atgtcacaga actattggaa gaatttcgat ccctcaataa aaccgcacct atttacattt
    15421 taggcatcta caatccattt tatttgaact ttcctgagat cactgacatg cagacaattg
    15481 ttaacaattg gaacaaagga acagaaacgg tgacaaaaaa aatgccgaac tgctacttta
    15541 tcccaatcaa tgatttgctt tatcaaggat tgaatcagga agtaggtgtc acagaagaag
    15601 gaacagatca aacaactagc acgacagaaa atctaaatgg catcaaaaat aatgtgctct
    15661 atgaagaaga tcatttccat ccaaataatt taggttatca attgatggca aatgcagtga
    15721 gagatcagct gatcgagaca caaaaccaat ggttagagaa ggaggatgat caataaacat
    15781 gaaaacaaga tctgacgtat cttcaaacaa aacaaagaaa acaaattact ggaaattcgc
    15841 cttccttatc cttcttgggg tagtacttgg ttcgaccgta tttttaggca gccgtatttt
    15901 tgctaatcgg gaaccagaac tcccagaaat accagctctt acggaaagac aaggagatcc
    15961 tgtcttaacg atcaattcaa ataaagaaaa agtcaatcaa atcatcagct ttttccttag
    16021 tgagtatcaa aaagatagtg atattgaata taaattttat ttggaaaatg aagcactact
    16081 caatggtact ttcgaagtac taggttttcc tatcgatttc tatttatatt ttgatccgta
    16141 tgtgatggaa aacggaaatg tccaactaaa agctagaagt ctttctatcg gaacattgag
    16201 tttgccaatc aaagatgtga tgaatatgat caagagaaac tataaattac cagattggat
    16261 cgaagtcaat acagatgatc tgactatcat gcttaggttg gatcaattcc gaatgcaaaa
    16321 cgggatgtat attaaagcag ataaaatcga cttagtaaat gatgacattc ggtttagtct
    16381 atatttgcca aaagatacct ctgatgcttc aaacaactca aataccaaca aaactagcac
    16441 cagcagtact gaaagcaaga caaaagaaaa taaaaaatca tcggatgagt agtattagag
    16501 ggggaatcat aatggaacga gcaatatttg caggaggctg cttctggtgt atgatccagc
    16561 ctttcgacac gcttcctggt atccatacag tgatgtcagg atatactgga gggcatgtac
    16621 cagatccgac ttatgaacaa gtaaaaagcc aaacgactgg acatacagaa gcagtggaga
    16681 ttatctatga tccaacattg atttcatatg aacaattagt agaactttac tggcagcaaa
    16741 ctgatccaac tgacgctttc ggacaattcg aggatcgcgg tgacaattat cgcccggtga
    16801 tcttttattt gacagaagaa cagcggatca ttgctgaaaa aagtaagaaa cagttagctg
    16861 aaagtggacg tttcgaacag ccaatcgtta caaaaatcga accagcccaa ccattttatc
    16921 tagcagaaga agaccatcaa gagtattata aaaagcatcc tgaaaatttt aaaagaaacc
    16981 atgcacgccg ggctgccttt atcgaagaaa attggaggaa cgaagggtga atcgaagctt
    17041 ttttcattat ttaatgacac tgcgaggcgg tggcacacat gatgctttat ctgttttcgc
    17101 tacagaagcc ggtaaagaca tccaatttcc taagcacagc tctagctacg aagaaatttc
    17161 taattatctg gaactgaatg tcgactattt gccgtccatg gatatctttg acgaagcatg
    17221 ggaaaaatat ttagaaaata atcatgacat ataagagata gttttgtact ttatgatcaa
    17281 aaaagttttt tgtccaatag cctaattgaa agaattattt ttatttcagc tatctagtcg
    17341 catttttttg atatttttct tataataaag aaagaatgaa aaaaagaggg atggattgat
    17401 agagattgac aaaaaaacaa cgctatatta atcgcttctg gcttgtattc aaatacttac
    17461 ttgtttctgt attccttgcg ttcatgttgc ggggattttt attcatcccg gtaccagtgg
    17521 aaggcaattc aatggaaaac gtgttgaagc aaggtgatat ggttgtgatg gaaaaatttt
    17581 cggagatcag acgatttgat attgttgttt ttcaattagc agatggcacg atttatatca
    17641 aacgagtaat cggtcttcca ggagaaaatg tcagctacca aaatgatcaa ttgaagatca
    17701 atgggaaagt agttaaggaa ccttatctaa ctaaaaacct gaaatcagat catgcaaatg
    17761 catcctatac gacggacttt acactacaag agctaacggg acaatccaag cttccggaag
    17821 ataattattt tgtacttgga gataatcgaa gagtttcaaa agatagtcgt tcgtttggca
    17881 cgatcaataa aactgatatt ctaggtaaag caagatttgt ctattatcca cttgatgaaa
    17941 taaaatggat ccagtgagaa acaataaagc gtgcagtctg ttgagactgc acgctttatt
    18001 gtttaatcca aaatcagttt actaccttag tacgtaaaat caaacagctc gtttaaacac
    18061 ttttctagcc tctaaagttt tgataaatga ataaacaacc attatttgaa tagggatttg
    18121 gatcatattc ctgatgaatc tacctagaaa aattactttg aaatctgcac caaacaaaac
    18181 agacatcagc caagaattga taaaaaggct gactgggatc ataatcaata attgggcagc
    18241 gatgacggaa ggcaggttta tctttttatg atataaaaat aagccattaa taaatccacc
    18301 aaggaaagcc gtgataagaa aagcagcttg aaacgttcct gaaccaaata tcgtgaaatt
    18361 taaagtgttc gacaacattt caagcaaacc agcccagatc ggtccatata gataacccat
    18421 aagcgccata cttaaaaatc cgaatgtgat ttgaaaataa gcaggaccaa tcgctatctt
    18481 gtataagatg acatcaaggg ctaataaaag agcagaggtc gacaacaatc ttgcattcat
    18541 ttcgggcaat ctaaaaacgt cttttgcaat caaagtgttc aaaatattct ctcctttgtc
    18601 agttaataat aaaaggatac cataaaatca ccaatgtgtc atgacacatt ggtgatttac
    18661 atacactggc atctaacaat taaaaaaagg gtgagaagac tgcttttata aactgtaaaa
    18721 aagagattgt acgaataaaa aaaatagagg cggagaatac ttatcgtatc atccgccttt
    18781 gtttttttgt tataaaaaac
//
LOCUS       DACDHL010000048        17648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDHL010000048 DACDHL010000000
VERSION     DACDHL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.048"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..579
                     /locus_tag="IV228_001735"
     CDS             79..579
                     /locus_tag="IV228_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8717627.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(769..1113)
                     /locus_tag="IV228_001736"
     CDS             complement(769..1113)
                     /locus_tag="IV228_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8717628.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1267..2187)
                     /locus_tag="IV228_001737"
     CDS             complement(1267..2187)
                     /locus_tag="IV228_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8717629.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2374..2865
                     /locus_tag="IV228_001738"
     CDS             2374..2865
                     /locus_tag="IV228_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8717630.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2889..5264)
                     /locus_tag="IV228_001739"
     CDS             complement(2889..5264)
                     /locus_tag="IV228_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8717631.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5590..6216
                     /locus_tag="IV228_001740"
     CDS             5590..6216
                     /locus_tag="IV228_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8717632.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6239..6943
                     /locus_tag="IV228_001741"
     CDS             6239..6943
                     /locus_tag="IV228_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8717633.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7135..7968
                     /locus_tag="IV228_001742"
     CDS             7135..7968
                     /locus_tag="IV228_001742"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8717634.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            8165..10093
                     /locus_tag="IV228_001743"
     CDS             8165..10093
                     /locus_tag="IV228_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8717635.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            10231..10686
                     /locus_tag="IV228_001744"
     CDS             10231..10686
                     /locus_tag="IV228_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8717636.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            11156..11425
                     /locus_tag="IV228_001745"
     CDS             11156..11425
                     /locus_tag="IV228_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717637.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            11825..12079
                     /locus_tag="IV228_001746"
     CDS             11825..12079
                     /locus_tag="IV228_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8717638.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(12168..13214)
                     /gene="holA"
                     /locus_tag="IV228_001747"
     CDS             complement(12168..13214)
                     /gene="holA"
                     /locus_tag="IV228_001747"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8717639.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFFSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(13252..15525)
                     /locus_tag="IV228_001748"
     CDS             complement(13252..15525)
                     /locus_tag="IV228_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8717640.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(15518..16057)
                     /locus_tag="IV228_001749"
     CDS             complement(15518..16057)
                     /locus_tag="IV228_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8717641.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(16193..16411)
                     /locus_tag="IV228_001750"
     CDS             complement(16193..16411)
                     /locus_tag="IV228_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8717642.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(16408..16653)
                     /locus_tag="IV228_001751"
     CDS             complement(16408..16653)
                     /locus_tag="IV228_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8717643.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(16769..17497)
                     /locus_tag="IV228_001752"
     CDS             complement(16769..17497)
                     /locus_tag="IV228_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8717644.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5442 a         3394 c         3126 g         5686 t
ORIGIN      
        1 aaaataggca tggctattta caacaatagt gctatagtag aaaacaaatg aataaggtta
       61 cttaaagagg tgaatacgat gaaactgatc aaactatggg aagcagattt aaagaaagct
      121 tatcagctac aaactagctt tggaccaaat gagagtggat ttatcaacaa tgcttatggc
      181 tatacatttg ctgaattttt agattatgta aaaaaatgcg aaaaaacgtc tttaggaatc
      241 gatcttcctg agagttacgt tcccgcaacc gtctttattt tagaaagtga tgaacatgag
      301 tatgtaggaa tttttaattt gcgccataaa ctgaacgaaa cattaagaaa cggcgctggc
      361 catattggtt acggcatctc ccctaattat cgtaaaaaag gatatgccac acaaggatta
      421 gcattagtac tacaagaggc aaaaaaaatc ggcattgatg aggcactttt gtctgtaaat
      481 aaaaaaaatc ttggcagtct taaagcacaa caaaaaaatg gggctgcgat ttatcgtgaa
      541 gatgagaaag aatactatac gaaaatcaaa ctgatgtaaa aaagaaggat tccaaaagga
      601 aagggatttg aatgtccttt atcaatagaa cacatttatt taattaactt ataatatagc
      661 agacaaaatt ttataaacaa agaaaccttt tatctctttt cttctagaaa ggataaaagg
      721 cttttttgtt tttcctattt gcttctcata gctagtcagc caagatttct atcgtatccc
      781 caacgcaatc cgtgcataac ggctcatctt atctgtcgtc catgctggat accagactag
      841 tttgacttct gctttcgtta cttctggcac ttcttttaat gcgtcatgga tagattctgt
      901 taaaatatct gctaatggac agcccatagt tgttagagtc atcttaatga ctgtttctcc
      961 attttcagga ttgaattcta tatcataaat caagcctaaa ttgacgatgt cgattcctaa
     1021 ttcaggatca atcaccattt ccaaggcagc aagaatacgt tctttgatta gttctatttc
     1081 ttgtccagac cgttcttggt ttgcttcgct catcgtacga cactccttaa cacgattttt
     1141 atttatcata ctcttttttt agctgcttgt aaatgagaat ttagaagtaa gattctcgtt
     1201 taaatttata aaaatgttct tattagatgt aatagattaa atttattcta aaataaacct
     1261 gattcctcat tttttttgtg aacgattttt tatttctggc aatacgccag ttgttcgata
     1321 cattttttgt tcgttttcat ttgcaaaccg gccaaatact gaatgagttg gtaaaatatt
     1381 gataaatgca tcgtcgtcga tttcatgaac gatctgatca agatcataca tttcgtagcg
     1441 ggtgatgacc atcatgatca tgttcccttg tttttttgaa tagccaccga cagacggcag
     1501 taatgtcatt ccacgtacca tccgttttgc tagttcttct gttacctctt ctggcttgac
     1561 ggtaacgatc attgctgtta ctttttgatg actcgtgtgg atggcatcaa ttacttgagt
     1621 cattgcataa atggaaataa tcgtatacaa ggcactttcc cagttaaaga agaagcctgc
     1681 gatgactaca atgataccat ttagaatcag catgtagttt ccgactgttt ttccagttgt
     1741 ttgtgaaagt acaagagaga tgatatccat cccaccagtt gtaaagccca tttttaaaga
     1801 taatcctacg ccaagaccta ataaaacacc gcctactagg gcattcatca aaatattatc
     1861 agtgatctgt ccgactggaa gcagcatcgt catcaccgat acacatacaa cattcacaaa
     1921 actaaggatc gttgctcgtt ttcctacttt gagaaaccct aatacaaaca cagggatatt
     1981 caaaagaaaa ataaatgttc ctgtgtctat cttgatatga aaccattcat ataaaagcga
     2041 ggaaatgatt tgagcgatcc cattcatccc tgcagaaaag acattggctg gaatcaaaaa
     2101 ataatttaaa gcgaccgcag cggtcaatcc ggttatcaaa ataaccacta ttttctttaa
     2161 tctttcattt ttatgatacg cagtcataat tttactcaca agcacctctc ccctactttg
     2221 gtattccaaa tgtaagaata acagtttatc tattaaaata aaatacttcg aactcaaatg
     2281 agaagaattt tagcaaacag gtaagaatct gccctaatcc tatgataaga tagatgtatg
     2341 gtaaatattt atatgtatat caggagggtt cctatggcaa aagatgctag cttcgacatt
     2401 gtatcagaag tcaatacaga agaagtaaaa aatgcgattc agcaaacaac aaaagaattg
     2461 tccaatcgct ttgacttcaa ggattctaca gtagaaatca aattagagaa cagcaatcta
     2521 gttgtattga gcgatgatga tttcaaaatc gaacagatca aagatgtatt atttggtaag
     2581 ctgatcaaaa gaaatgtgcc aatcaaaaat atccattttt ccgaatcaga acacgctcta
     2641 ggtggcaaag ctcgacaaac tgccgatttg gtcaacggaa tcgatcgtga aaacgcgaag
     2701 aaaatcacta cagctatcaa aaatgaaaaa ctgaaagtaa aagcacaaat ccaagaagat
     2761 cagattagag tgacaggtaa gaaccgtgat gatctacaag ctgtgattgc tttattacgt
     2821 aaacttgact taccaatcga gttgcaattc actaactatc gttaatcaaa aaacgaggct
     2881 gacgtctgtc attcctcgtt tttttgattt actgcttctt ttgataacca aaaacgctgt
     2941 attccttctt ccaattcccc aacacttcgt acttctcttg ctgtttgcca atgttgtggg
     3001 aataaacgtc gataagaaaa actagaaaat cgttgatctg ccaatacaac gattccttga
     3061 tccgccatgc cacgaatcac tcgaccacca gcttgcaaga ctttgttcat tccaggaagc
     3121 tgataagcgt aagcaaatcc aaggtgttcc ttctcatcat aatatgattt gatcagttcc
     3181 tgctcatgat tcatctgcgg caaacctaca cctacgatca tactgccgat caatcggtcc
     3241 cctctcaaat cgattccttc tgaaaaaatc ccgccaagta cgcaaaatcc aatcaatgtt
     3301 tcagcgggat ttgtttgaaa ttttgttaaa aatcgttcgc gttcttcctc attcatgtcc
     3361 gtttcttgga ttagcacatt gctgtcggga tattgctgtc tgaatgcttc caccgcaaga
     3421 tctaaatatt gataagaagg aaagaaaacg aaataattcc cgacctttcc atcagcaaaa
     3481 gcatgaatcg tctctactaa ttttgccaga ctgtgttccc tttttcgata agtcgtctct
     3541 aggtaatttg cgatgaaaac ttgctggttt ccttctggga aaggactcgg cagtttgtaa
     3601 gccagacttt tttgtccccc cagtgtttcc tgataataag agagtggtga aaaactagca
     3661 gaaaaaagaa tactgctttt tcctttatcc aacgactgct caagaaacaa gcttggatca
     3721 atacaaaact cttttactat cagatcgcga tacgtttttt cgactgttgt ttcgtagtga
     3781 tcatcataaa attcactgac cttcaaaaaa tgaagcaaat caaaataata aggaagcaat
     3841 tgttcttgtg atgggtgctc aggaaattct gccagccatt ctttaatttt ttcgcttaat
     3901 tgataacctg cctttactaa agattcagcc ggtgcttttt gatgatgata atcccaatga
     3961 tcttcttttg ctatttctcg gatcgaatca aattctttta atagtttgtt gaaacggcga
     4021 tgaagttttt tgaattcctt tggtatagct tccttacttc gtttcgtttt ttcataagat
     4081 agttctgccg aatacatctc tcgggaacga ttcaccaaat tatgtgcttc gtcaatcaaa
     4141 aacagatagt cctcgttttt ttcatcttca aaaaaacgtc ttaaataaac agttggatca
     4201 aataaataat tgtaatcccc aatgattacg tcacaccaga tactgacatc taatgatagt
     4261 tcgaaaggac aaagtgtatg ctttcttgca taagtttcga tgacttcacg agtgatctga
     4321 ttctcatgat tcaaaagatc ccataaacct tcattaatcc gattgtaata accattggca
     4381 tatgggcact gatctggatt acaggtcgtc tcatctaaaa agcagatctt gtctttcgcc
     4441 gtcaaagtga cactttttac ttcagctcct gtatctttta atgctgtcat agcatcttca
     4501 gctacttgtc tagttattgt tttagccgtt agataaaaga tcttttctcc ctcttcttcc
     4561 cctacagctt tcaaagcggg gaataacgta gagattgttt ttcctgttcc ggtaggtgct
     4621 tctacaaaca atttctgttt cgtacggatc gttttataag cagctaccgc tagttcacgc
     4681 tgacctttcc tatactcatc atgggggaat ttcaaagcca tcaaagaggc attgcgtaca
     4741 gtgcgccagt tttcttgaaa aacaagccat tctttataat cttcagtcag ctttttaaaa
     4801 aaagtttcta actgctctct tgtctgatgt tcaacctttc tcgtgatcac ttcttctgcc
     4861 gtctggaagt aagtcaattg cagatttatt tcttgtaaat catgttcttg actatagata
     4921 taggcataca cccttgcttg atgaaagaaa agttccactt gttctggttc taaatcttca
     4981 aatcgtggtt cggaggtttt gatttcatct atataataaa taccgtctct tttgaaaata
     5041 ccgtctgcac ggccttcaac gatcaggtaa tattcatcta attgaactgt tgtttttaaa
     5101 aagacttctt tttcatagtt ttctcccgca tctttttgga gcttgcggtg gattctcgct
     5161 ccttctaatg cagtatggtt acttttttta cgattgtcga tacttccacg tcgcaaaata
     5221 aattctacta agtgccgcac agatattttt gactgtttga tcatttccac cacctctttc
     5281 tctctaagat tacctgaaaa aacctagttt agcaaaaaga gctctctttc caaacataaa
     5341 aaaacaccct gctgattagg cagggatata ggatatagaa tttggatttt tggatgaatc
     5401 taatgtaaca aggacatatt aaaatgctat gaaaacttac tttccttctc tttagaatat
     5461 ccaaatactt ttcatatagt ctgataattc agataagtaa agagaaataa gtacctgttt
     5521 ttgtttccta gcaatgaatg cgttatacta atggatgaaa gaagttttgg ctatcaagga
     5581 ggaatgcaca tgcatatcga aagattaaaa acaggaatga ttgaagaaaa ctgttatttg
     5641 gtctacaacg aagaagcact tttgatcatt gaccctggag aagatgcgga acgaatcaaa
     5701 caagaaatca aaaaaacaaa ccaacagccg gtagcgattt tgcttaccca tactcactac
     5761 gatcatatcg gtgcagtgga acctttacgc gcgtattatc aaatcccagt ttatgtcagc
     5821 ccactagagc aaaaatggct aggtgatccg attttgaatc tttctggact cggtagacat
     5881 gatgatattg ctgatgttat tgtctcgcct gcagaatatg aatttgaaat gaaaacttat
     5941 actttaggaa acatgtcttt tcgcgtcgtc cctaccccag ggcattctat aggaagcgtc
     6001 agctttattt ttgatgattt tgtcgtttct ggcgacgccc tgtttagagg aagtatcggt
     6061 agaaccgatc tgtatacagg gaatttagaa caattacttc atagtattcg gacccagctg
     6121 tttgtattac ctgatgaatt tgcagtctat ccaggacatg gcgatgcaac gacgattgaa
     6181 caggaaaaaa ggacaaatcc ttttttcaat cgttaaaacg acttaggagg agaataaaat
     6241 gacagaaact acactttata tcgtacgaca cggaaaaacg atgttcaata caattgaacg
     6301 agtacaaggc tggtgtgata cacccctcac aaagcaagga caagaaggca ttcattattt
     6361 aggtcgcggg ttaaaaaatg tcgacttttc atttgcttat tctagtgaca gtggtcgtgc
     6421 aatcgaaact gcccgtatta ttctaggaga acattctaag ggaaaagaaa ttccttattt
     6481 tattgaaaaa aggattagag aatggtgttt cgggtctctt gaaggtggat atgacatgga
     6541 aatgtggggt gtgatccctc gtgtattgaa ttttaaaaca tatgatgaaa tgtttaccac
     6601 agaagtttct tttgaacaaa ttgctaatgc tgtatacaac ttagataccg ctgggtgggc
     6661 ggaaacttat gaaacattgc gagaccgtgt atggacagga tttgaagata tcgcccatca
     6721 ctgcgaaaaa aatggtggtg gaaatgtttt agtcgtatct catggtttga cgatcgcctt
     6781 tttgcttaat ctgatcgatc ctaaacagcc tgttcgtgct gggcttttaa atggcagcgt
     6841 aacaaaggtc acttatgcaa acggtcgatt ttctatccac ggcatcaatg atacgtctta
     6901 tatcgataac gggaaaacac atagtcgcga ctcgtttcta taatggggat attttaattt
     6961 ttaccagaga atcataaaag gcaaagtctg aacgagggct ttgtcttctt ttttctcttc
     7021 gtgtacttat ctgctttatt tctcaagttt ttcttaatat taaaaaacag attgggtttc
     7081 tcaaaaaata acggtatact ataagatgta tcttaattgg gaaggaagga atttatgttt
     7141 tttgcaaata ttatcaaagc agtgattctc ggtattattg aaggaataac ggaatgggtg
     7201 cccatcagta gtactggtca tttgatccta gcagatgaat tcatcaaact ggatgccagt
     7261 tcacaattta tgtcgatgtt caatgtcgtt atccaattag gggcgatctt ggctgtcgtc
     7321 gttctttatt ttcacaagtt aaacccattt tctccaagta aatcaacggt agaaaaaaaa
     7381 gatacgtgga cactttggtt taaagtgatc gttgcttgca taccagccat catcattggt
     7441 ttgcctttgg atgattggtt agaagcaaat ttccacaagt tcttaccagt tgccattgtt
     7501 ttgatcgttt acggtattgc ctttattatt gtcgaaaaac gcaacaaaaa caaaacacct
     7561 cgttggactt ctctgaatga ctttacgtac caagcagctc ttcttgttgg gttattccaa
     7621 gtcttggcac ttgttcccgg aacatcccgt tctggcgcaa cgatcctagg atctatcttg
     7681 attggtgctt cgcgttttgt cgctacagag ttttcatttt tcttagggat tcctgtcatg
     7741 tttggcgcaa gcggcttgaa gatcgtcaaa tatttagctg aagggaattc attcaaaatg
     7801 gaagaaactg ttgttcttct tgtaggaaca ttcgtatctt ttgtggtttc cgtcttagct
     7861 atcaaattct tactaagcta tttgaagaaa aacgacttta ccgcttttgg ctggtatcgt
     7921 attatccttg gtatcatttt gattggttat tggtttattg caatgtagaa aaaaccagaa
     7981 cacttctctt atttgagaaa tgttctggtt ttttattttc tttaataata atcattataa
     8041 ataataaaaa caacatcaag cttaatcacc aaatgataac cattatcgtt ctcatttagc
     8101 tgttattatt atcaattaca tttttattat gctatccttg ttttgacaag gaggtctaat
     8161 aacgatgact aatcagaaaa aagcttttgc agcaacaatc ggctgccttt tatttatgat
     8221 cttcggtttt atcttcgacc gattctctct taccatcacc ccactttttt atatcagtgc
     8281 cattatcatc ggtggatata aacaaacatc ggaaggcttg aaagaattgt ggaatgatcg
     8341 gacactgaat gtggatttat tgatggcttt agctgctatt ggtgcctgtt tgatcggtaa
     8401 ttactttgaa ggagcaatgt tgacattcat cttttgttta agcggtgcat tagaggaata
     8461 cacaactaat aaaagtcaaa aagaaattac tgctttgatg aatcttcaac cacaaaaagc
     8521 ccaattatta aaagaaaatg gacaaatgca agaagtagat gtttctcaac taaaggtgga
     8581 taacttggta tttgtcgcta aaggagcagc tgttccgata gatggctttt tagaaagcac
     8641 acaggcaacg atcgatgagt ctgcattaag cggtgaatct gtgcctgtag aaaaatattt
     8701 aggcgatgag atgtatgcag gaacgctgaa tcaaggaaat cccatcacga ttcgagtaac
     8761 aaaaacgagt tccgaaactg tctttgctaa gattatccaa ttagtagaag aagcgcagaa
     8821 tacgccaact caaacagctt catttatcga acgaatcgaa aataattatg taaaattgat
     8881 tttgcttgct gttcctctaa tgatccttct cccccacttt ttcctaggtt ggagttggga
     8941 tgaaagcttt tatcgaggaa tggtattatt agttgtggct tctccttgtg cgcttgtcgc
     9001 atctgctaca ccagcttctc tagctgctct ttcaaattct gcaaaaaatg ggattttaat
     9061 caaaggcggt atccatttag aaaagctttc tgaattaaaa gcgattgcct ttgataaaac
     9121 aggaacgctc actaaaggaa aaccagttgt aactgacagc tttttctttg atgaaaagga
     9181 tttggcacaa caacttcttg ttgctatgga acaaaaaacg acgcatccac ttgctcatgc
     9241 gattttgcaa tatttcgatt gcacaatacc tgaagaaatc caatacttac caatagaaga
     9301 aattactggt tttggattac aaactaccta tttaggtgcg caatggaaag ttggcaagca
     9361 cgcatatgat cctgagacga tgataatcag caaagagatt gcggaaatga tcgaacgttt
     9421 ggaaaatcaa ggcaaaaccg tgatttatct ttctaaagat cagcagttaa ttgctgtttt
     9481 aggtctgcta gatattccaa aagcaaatac ccaacaagta atttcctact ttaaatccca
     9541 aaacatccat acaagtatga taaccggcga ccattctggc acagcaaagg ccattgctga
     9601 acaagtaggg attgaacatt actatgcaag ctgtactccg gaagaaaaaa cacaactagt
     9661 caaaaaagag aatgaacttt accaagtaaa tgcgatggtt ggtgatggag tcaatgatgc
     9721 gcctgcttta gcagccgctt ctttaggtgt cgctatgggg caagggacag atgccgctat
     9781 ggatatcgca gacatcgtct taatgaaaag cgacttaaat aaatttgtct acagtcatcg
     9841 gctatcttta aaaatgcggc ggatcatcaa acaaaatatt attttttcga ttcttgttat
     9901 tctcttgttg attgcttcta acttcctgca atttttaaat ctacctcttg gagttgtagg
     9961 acacgaagga agcacaatat tggttattct gaatggcttg cgtttactaa gacctttacc
    10021 tgatctttct gattcttcaa aaaaatgtaa gtcttgtcca ctatacaaag cgcggctgtc
    10081 ttctgtcaaa taagcaaagt gtttttttat gattttgttt attttatgaa aaaaacagct
    10141 tatgaatagg tcttgaagga tgaattccta cttttgtaac tttctaaaaa catgttatga
    10201 ttgtttcgaa gaaagtgagg aatatatttc atggaaagtt ggcttttttt aggaattatt
    10261 ttacttgtag caattttagc taaaaatcaa agtctgatga tagcggcagc ttttgttttg
    10321 attatgaagc tcataccagc ctcccgttcc tttttccctt ggcttcaagg caaaggaatc
    10381 aatattgggg tgacgatcat cacagcggct attctaatcc ctatcgcaac aggagaaatc
    10441 ggtctacgtg atctattaga agcctttaaa tcccctattg gctgggtagc tgttttttgc
    10501 ggtgggctag tggctgtctt atcttccaag ggtgtaggat tgattgcttc tgatccacaa
    10561 atcactgttg cacttgtttt cggcacgatt ttaggtgttg tcttcttaag aggaatcgcc
    10621 gccggaccgg tcattgcttc tggtatcact tactgtatct tacaagtatt tgctttagga
    10681 aaatagaaca aattttgtct attttaggag attcttaaca cccccaattt actgatccgt
    10741 gatatactga caacgaggtg atacaatgtt cgaaaaatta aagtatttca taggaacaat
    10801 cggctggttt cttctagcgg tacttttgat tctgggctta gcttatctat tatttcctga
    10861 tttgatacaa aatgtgtttg atactgcttt ttgattgacc tattaaaacg atatagggga
    10921 tgctcctgcg atgcaggggc tttttttaat caatgttttg taataattta ggccgtgcca
    10981 atgagacttc ctatagaatt cgatgctctt ttttcttttt ggctcttctg ctcttttttg
    11041 ttatactaga ttacagtgga caaaagaaag aatgatcgca tccacttcaa cgaatatcaa
    11101 aatggaagaa ttagtgcctg ttttcaggtt actttgaaaa gaaaggaaga cgcttgtgga
    11161 aacttttgaa ggtcttttaa ttgatacgta cgttttacaa gaacagccaa tgatgctgac
    11221 tgggatcaac ttgcagcttg aggaccgtgt gatcaatggc ctgcttaaag atcgctattt
    11281 ggcaaaatat atcttacgtt tgcccctaaa taaatatgtg ctggaagtaa gtggcaaatg
    11341 gaaccgtcgg aaccagttgg tcataaaaaa aatgttgata aaaaatatcg atgaatacat
    11401 taaaataatc ggtacaccag aatagccttt actgatcaaa ggatactggt tataaaataa
    11461 ctgcagtttc ttaatagaaa aacaaagaac aacagggaat cgtatccctt ttcaaaaaaa
    11521 actttgcaaa cggttctctt tgttatataa taacttagta ccaaacattc ccattcaaac
    11581 tttgtttaaa tagataaata aaaatctagt ttggtttgac gctattgcct cccacaatag
    11641 cgtctttttg atttttttca gcttgtacta tccgttttta ctgaaaaaaa atcctaataa
    11701 aagactgtta aggaaaaaaa cttgtcatgt tcatattgca aagcacgctg cctttaggta
    11761 taataatgaa tgttgaatta tgcggtaaca cgcgtaagaa cttaagttat cggaggtgaa
    11821 actaatgcca aacattgaat ctgcaatcaa acgtgttcgt acaagcgaaa acgctaatgt
    11881 gaaaaattca tctcaaacaa gcgctatgcg tactgcaatc aaaaaatttg aagatgctgt
    11941 agcttcaggt gctgacaacg tagatgcgtt atataaagaa gctgttaaag ctattgatat
    12001 ggctgaatca aaaggtctaa tccacaaaaa caaagctaac cgcgacaaat ctcgtttaag
    12061 caaaaaaatc gctaaataaa agaatcggct tcggccgatt tttttatttg tcaaaaaaag
    12121 gacaaaaaca aacgaccttc ccgcatgctt tgccctcgtc tttcttctta tcgatttctt
    12181 aacaactgcc ctgtgcgcaa aacgaatagt tgaaaactta attctttttc gatttgtccc
    12241 gttttgattt gataatccat ctcaactaat tgatcaaaca actgtaccag caaatgttca
    12301 tcgaattttt ttacttcctg catggcaagt tttacacgat atggatggat tttcaatgtt
    12361 tcagcaatat ttgcctgctg ataaccaatc tttacaagaa attttgtctg taaaaataaa
    12421 cgtaattgag aaagcaaaat cgcattaatt ttgattgttt cttccccttg cataactaaa
    12481 tcttcgtata aacgcaaagc ttgttccgtc ttccctttta aaatatattg ggtcatgtca
    12541 aaaatattat gttctaacgt tttaggaact aattcttcta cttctcttac cgtgatttgc
    12601 tgttgatttt gtccataaag gaccaatttc tgtaactctc tcatcatttt ggataaatct
    12661 aagtcggtca acctcaagaa taaatcaatt gcacttcgtt ccattttgat tgaggaattc
    12721 tctaaggtat tcatcagata ttgacgtact tctttttcat tcaactgttg gacattgatg
    12781 ataattgctg tttttttcaa ttgtttcgtt acttttttcc gttcatccaa cttttcatat
    12841 ggcgcaaaga atactaatac agtcgattct aatggttctt tcaaataagc cgtcaataaa
    12901 tcagtatttt gttcgattcc attgtttact ttttcacctg ttaaaaaata aggattctct
    12961 acgaatacta accgttgatc tccgaaaaac ggcaacgtct ctgcttcagc tactacctca
    13021 tctaaacttg cattttccat gtcaaaacta aaaaaattta agtcatcttc gccctctaat
    13081 tggatttttt tgacaatttc tgtccgaact tgatcttgga gaaacttttc tgttcctaaa
    13141 atcaagtaac aagatgcaag tttttcttta cgtatttttt gtaactctgc ttgtatactc
    13201 aaattactca ccactttcaa aaattatcct accatgagca gatgagaatt actagtctat
    13261 cattgtttcc actagcccat gaggatgaaa aactggatgc cagcgatagg tgatcattcc
    13321 ttgttgatcc gttcgccatg tttttatctg attttcttct agtgtctcca atacttcgct
    13381 gtgcgggtga ccataacgat tatttacgcc gcaggatata atcccatgtt ttgcttcaat
    13441 atgggaaaga aaatctgtgt tacttgacgt acggctaccg tgatgaccta atttgactac
    13501 atcggctttc agattaggat aaatcaaaag caattgtttt tccattgctt gattcaaatc
    13561 tcctaaaaat aaaaagcgcc tattgttata ttccatccat agaccaatcg aatcttcatt
    13621 tcttccctct cccttttcaa aaggagtcaa tacataaaga tcataataac cgctgatcct
    13681 gtccccatgt ttgatttcaa tgatctcagt accatttttt tgagcttcag ataaatgcct
    13741 ttgtaatggc tcatttcgtc cagcgcccca accgatataa atttttttga tagtaaaatg
    13801 ctgagataac gtgggtaatt ctcccacatg atccgtatcg tcgtgtgtca agatgagttt
    13861 atcgattaca cttatcccct gaccttttag atatggaacg atatttcttt cagcaggagg
    13921 acgtatcata ccttgcgccc acttttctct ttcaaagtgt aactgacctc cagtatcgat
    13981 caaaatcgtt tcttttcgaa aaggactttg caaaaagata ctgtctcctt gaccgacatc
    14041 gataaaagcg atcgttgaag aaaagcgtaa aaatggcaaa gtcaaaaaaa taaaagaaaa
    14101 aagaccaaca taaaacaagt gtttcacagg ttgttctttc aacctatcca tacaaatcag
    14161 taatccgaaa atatacatcc cagatagcac aaggggcaaa cgtcctataa tgagagacga
    14221 ccctgaagaa aaatcgatta ttcgctcaaa taagattaat ccctcttcta tcagcaaaag
    14281 taaagtttcc ggcagccagc gattgaagag tgtgagaaat aacacaccag gaagaaaaag
    14341 aaacttgaat accggtgcaa tcagtaatgt aaaaagacta cctaaaatag gccactcata
    14401 aaaatgccat acaatcaacg gcatcgtgat catagtaaac aggacgtgag aggccgtttt
    14461 tttcaaccaa tctccatcaa aagaaatcag gataaacaaa aaactcatga aaaaactaag
    14521 ctgtccaccc gcatgaaaaa gaaatcgagg atcaaatagt aaaagaagcc aaaagacaac
    14581 tgcaaataag tccatggtgg aataatgctt attcagcaat ttgactagca atcgcaataa
    14641 aaacagcaaa ctagcacgca aaacagaaat agaacttcct gcaatagaaa cataacagat
    14701 agttagtaga ctcaacggaa taatactgac gtgtagtggc catccgatac gtcgaaataa
    14761 ataatacagc caccctaaat aaaattggat gtgtagaccc gatagactga acagatgcag
    14821 caaaccactc tccctaaaaa tttcttctgg ttctctgaat cctgcatctt tatagcccca
    14881 taataaagaa ttaatatata cacttgtttt ttgcggaaat tcagattgga caaaggagat
    14941 aaaagatcct ctttttctct gaagccagtt tcgccagtga ttttgcatgg tgatctgttc
    15001 aatcttttga atttggaacc gtccagaaat tctatggttc ctgaagtaag cttcttggtc
    15061 aaaattataa agattccgct tttttgctgg agagatgaac tcgccttcag cagtcaattc
    15121 tattcccttt cctcggaagt taagccatgt tttcaattct gcttctgtgt ttacttgata
    15181 agaaagctgg agcttttgtt tattctctga taaccttcct tccattgtca aaaaattacc
    15241 atttgcacga atcgtatctg taaatacagt aatttgcgca gatgttgttt tttcctctaa
    15301 aaatgaatac tcttgtagcc tataagaaat ggataccgtt atgctacaca aaatacacaa
    15361 gtacgcgata aaacgtcctt cttttagcga aaaccataaa aaaattccgg caagaagcaa
    15421 acgataaccc caattgtcag tgtaatacaa caaacagata atcatcgacg taattgccgg
    15481 aaaaatcaac cgatttcgaa catgcctttc taaaacgcta gacatgtttt cctgttaatt
    15541 cattttgaag ttttgaaaaa tgctgtgagt ctaaagcaac ttggtgtaca gatacaccta
    15601 attgttcgat caacgcttgg gcataaggat catttcgata gtcatggaga taattgatcc
    15661 ctttcacacc agcttgcaac aaagcctttg tacaggccaa acatggaaaa tgtgtgacat
    15721 agatttctga accttccgtc gggataccaa gttttgcaca ctgtaggagt gcattcatct
    15781 ctgcatggat cgttcgcaag cagtgtccat ccacgacgta acacccttca tctatgcagt
    15841 ggacatctcc actgactgct ccattatagc cgccagcaat gatccttttt tctcgaacga
    15901 gtgtcgctcc tacttctaaa cgtgtacacg tactacgtaa agataacagt actgcctgag
    15961 ccataaagta ctggtcccaa ggaatgcgtt cattatgtga cttatcagta actttttctt
    16021 tattcgtatc gcatgctttt gaagattttt ctaacatgtt ttatattcct ccgttttttc
    16081 aagttgatta aaaagtactc attgcttaaa caaaatattc tttaaatata acgaatcatc
    16141 ctgtctgaca aaagatccta cccttcactg ccgaaatgaa gtccgtcatc cattagccca
    16201 ttataaaaat tcttcgaatc catttgttca agaaattccg tataaaaatc gatacttcac
    16261 aatcatggcg ttttgctact tctgtgagaa cagagatata ttctttgatc aacttgttat
    16321 tccgatattt ttgttttgtc tcatcgactg ctggtggcgt gataaagaaa gcatgatcag
    16381 aactatagtc ttttttcagc tgaactatca tccactgcag attttttggt acgtttcttt
    16441 agaaagttgt ttatgctctg ctagatcatt cgtcccaatg agaataaata ctttatctgc
    16501 tgtatagcta agtacatctt tggcaaagcg ttctcgtaga gcttgactgt tattgccgct
    16561 gacagccgta ttcataatat gaatcccaga tattttattt tggagataga cattcagcat
    16621 tggttttttt aatgtttctt tgcgaacaat caagctattt cctatccaaa aatactacct
    16681 cctcgtcctg ccaagctctt agaattggat tattcttagt atccattagc gtccatttat
    16741 tgctcgtgcc agtcgatttt gaatcattct atttaattgt aacgaagttt tttagttttt
    16801 caactgtctt ttcaccgata ccagaaatct cctgcaggtc ttccactttc tggaaggatc
    16861 cgttttcttc tctaaatcga atgatttctt gcgctttgac tgatccgatc ccaggaatct
    16921 gctgaagttc tgatggagtg gctgtattga tatttatttt gggaggctcg gaatctgctg
    16981 taggctgttt tccttccgag gatgccaact gatcaagatg ttctggcagc tcttcaccta
    17041 cagcagggat atataggact tgctgatctt ctatcttcgc agaaaagttg attcgatcgc
    17101 tatccgcttc ttctaataat ccacctgcac gctttagtac atcatagacc cgttcttctg
    17161 aagaaaagga atagatacca ggctccttca cacttccttt gatatcggtg tacatgacct
    17221 ttggacgagc atcctcttct acatcgtttc cttcagctga tggatcttct ccactggaac
    17281 taaacgaaga aagtatttct tttgcagcag tctcttctgt ttctctataa aatagcaaat
    17341 ttgataataa aaaaccactt gcgatacaaa agataagaac tatcatagac atccaaatga
    17401 tcaatttggg tgaagctagt attttcttgc tgattttttc tatcattgtt cttttttcaa
    17461 agaatgacca gtaaaaatca ttcattactc ctttcattta aaattgaaga aacagtgagc
    17521 tctggcaaaa tcgtcccttc aacatctaat tacgtcaatt ttttcttttg tttttatttt
    17581 tatcgtgaag atttttgacg aaaaagaggg gttgtgacag aactcgtgtc acaacccctc
    17641 tttaatta
//
LOCUS       DACDHL010000049        17351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDHL010000049 DACDHL010000000
VERSION     DACDHL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.049"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            64..546
                     /locus_tag="IV228_001753"
     CDS             64..546
                     /locus_tag="IV228_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717645.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            743..1633
                     /gene="pstS"
                     /locus_tag="IV228_001754"
     CDS             743..1633
                     /gene="pstS"
                     /locus_tag="IV228_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8717646.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1920..3194)
                     /locus_tag="IV228_001755"
     CDS             complement(1920..3194)
                     /locus_tag="IV228_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8717647.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            3467..5938
                     /locus_tag="IV228_001756"
     CDS             3467..5938
                     /locus_tag="IV228_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8717648.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6041..7777)
                     /locus_tag="IV228_001757"
     CDS             complement(6041..7777)
                     /locus_tag="IV228_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8717649.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7774..8478)
                     /locus_tag="IV228_001758"
     CDS             complement(7774..8478)
                     /locus_tag="IV228_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8717650.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8609..9112
                     /locus_tag="IV228_001759"
     CDS             8609..9112
                     /locus_tag="IV228_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8717651.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9072..9410
                     /locus_tag="IV228_001760"
     CDS             9072..9410
                     /locus_tag="IV228_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8717652.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9431..10849
                     /gene="ffh"
                     /locus_tag="IV228_001761"
     CDS             9431..10849
                     /gene="ffh"
                     /locus_tag="IV228_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8717653.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10961..11539
                     /locus_tag="IV228_001762"
     CDS             10961..11539
                     /locus_tag="IV228_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8717654.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11543..12064
                     /locus_tag="IV228_001763"
     CDS             11543..12064
                     /locus_tag="IV228_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8717655.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12143..12697)
                     /locus_tag="IV228_001764"
     CDS             complement(12143..12697)
                     /locus_tag="IV228_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8717656.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12950..13225
                     /gene="rpsP"
                     /locus_tag="IV228_001765"
     CDS             12950..13225
                     /gene="rpsP"
                     /locus_tag="IV228_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8717657.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13237..13488
                     /locus_tag="IV228_001766"
     CDS             13237..13488
                     /locus_tag="IV228_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8717658.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13613..14404
                     /locus_tag="IV228_001767"
     CDS             13613..14404
                     /locus_tag="IV228_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717659.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14574..15095
                     /gene="rimM"
                     /locus_tag="IV228_001768"
     CDS             14574..15095
                     /gene="rimM"
                     /locus_tag="IV228_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8717660.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15085..15846
                     /gene="trmD"
                     /locus_tag="IV228_001769"
     CDS             15085..15846
                     /gene="trmD"
                     /locus_tag="IV228_001769"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8717661.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15982..16329
                     /gene="rplS"
                     /locus_tag="IV228_001770"
     CDS             15982..16329
                     /gene="rplS"
                     /locus_tag="IV228_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8717662.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16587..16880
                     /locus_tag="IV228_001771"
     CDS             16587..16880
                     /locus_tag="IV228_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8717663.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5723 a         3119 c         3564 g         4945 t
ORIGIN      
        1 taattttttt agatagaaaa ttgtatagta tgatgagaga aaaagtagtt tgggggaacg
       61 ttaatgagaa gaaaagcagt aatcgatcgg ctttatttat ttttttggtt ttttcctatc
      121 gttttgctac cttttacatg ggtaaatgct gttgtacaag gtgcggagat attaggcctg
      181 atccaaggaa gcggatttgt ggtttctagg aattttccag ctttgattgt tttctattta
      241 gtgataagtg taggtctctt attaccaaaa tctctgatca atcactacaa ttggcagctg
      301 ctttactttt cttgcttatt agtatttaca tctatgtttt ctttgctgcc tcgcttgatt
      361 atcggcccta atggtttgag cgagagtgga ttgaaggatt tagcttattc ctccatgtat
      421 ttcttgacga tcaaaccatg gttctattta gtaattgcct catttattct tgcaacattt
      481 ttttacttct ggacagaaat caggataaaa agtgaacgat ctgtatctat aaaaaataac
      541 tattgatggt ggatcatcag taaaaaaaca tatgagctgc gtaaaatgca tgtttctcat
      601 atgctttttt tattattatg taaagaatgc gaaaaacgtt tacatgattt ttacataact
      661 taactatatt tttacagcaa ccatacgagg gatttacagt cgactgataa actttagttg
      721 taaataagaa aggagcaata aaatgaaaaa agtattgttt actctaggta tgatgggaat
      781 tcttttagcc ggatgcggct caggatcagg aaatacagat ggctctgcca cacaaaataa
      841 ttcaagtgac tcaaatgaac aagtaaaaat cgttgcggta ggttcgacag cattacagcc
      901 gttagtcgat gcagcacaag aaagctttgt acaagaaaat ccaaattatc aaatctctgt
      961 acaaggagga ggaagcggaa ctggtttgag ccaagtcgaa gctggagcag ttactatcgg
     1021 aaactctgat gtatttgcag aagaaagaga cggagttgat gcttcaaaat tagtggatca
     1081 taaagtagca gtggtcggga tggcaccaat cgtcaataaa gatacagatg tcaaagatat
     1141 caccaaacag gaattgattg atatctttac aggaaaaatc actaactgga aggaagttgg
     1201 cgggaaagat caaaaaatca acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac
     1261 attcgaaaaa tggggactgg atggtgctac ccctgtgcag tcccaagaac aagattcatc
     1321 aggaacagtt cggcagctgg taagtcaaac accaggagca atcagctatt tagctttctc
     1381 atatctcgat gattccacac aagcactaag tatcgacggt gtagaaccaa aagaagagaa
     1441 tgtagcagat aacagctggg gaatctggtc atatgaacat atgtacacga acggaaaacc
     1501 ttcccctgaa gtccaaaaat tcttagacta catgatgaca gaagaaatcc aagaaggacc
     1561 tgtcaaagaa ttgggctatt taccaatcac aatgatggaa gtagaacgtg atcacgaagg
     1621 aaatatcaaa taactagcag ataaacatgc cacggtagtc gttactatta aggaaaaatc
     1681 aactatgttt ccccacatat acatttttcc tttaattgtg aaaaatagtt cctcatattt
     1741 tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat tcgaaaaaaa
     1801 gagctacgac atagaactgt cgtaactttt tttggctctt cgtcaaatcg tgttggtgaa
     1861 gacaaatttg aataaaactt agtccgatat gcggcgcgtt ctgtgcgccg catatttttt
     1921 tagttttcaa agatcagacc ttgttgtaaa tagatccgtg cccgaaagtt ataaaagttg
     1981 cgataaccaa acgcaattcg tttaatcagc ttgatcttgt tattcagccc ctcagtgacg
     2041 ccgtttgagt gggacacctt aaaggcattt gaaatacccg attggtagca atggaagatc
     2101 gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa ttgttttaag ggtttcttga
     2161 aagccttccg tacctcgttt aacgaaataa tagcgtaata attgacaaaa atgatacgca
     2221 gttcgcaatt cttcgttgta gcttaacagc tcatcaacga tttctgtttg actaattggt
     2281 cgtttaaaca gtcgatgata atgccgtttg agtggctccc atgtcgtgga atctttgagt
     2341 aagagtttcc aatatcgctt gagccgacgg tacttcttct gattttcaga gtgtgaggtt
     2401 cgaaaacgat tcatgatctg gacacgtaat tgattgaagc tgcgattgat gtgttggaca
     2461 atatgaaagc ggtcagttac gatctccgca tttggaaaga ctgttttaag caactgacaa
     2521 tagctggcat tcatgtccat cactaggtac ttgaccttta aacgggcctt tctaggaaag
     2581 cgcaggaagt attgagttag tttgtaaagt ctgcgatctt caaggatcgt aaatagctga
     2641 ttggttgtcc catcaacaca aataaaactc atggaaccag aaacagaatt cattgatttg
     2701 aactcatcca tacacaacac cgtcggaagg aagttcatac gtggctggta atgcttcgtt
     2761 gcttcacgca agatccgtgt caccgtccca tccgagacga agtacttctt agcgattagt
     2821 ttacgtgatt gattttcgta cagttccatg agaatcgcct gcttcagttc ctttgttagt
     2881 tgatgatgtt catcaactaa gtccgttttc gcagaaaagg tccttccaca atcatggcag
     2941 agaaaacggg tacgttttaa ttctaaaata gttaactttt ctttgacttt caacatctga
     3001 gtgcgtgtcg tataggaacc gttgcggata atacgaaccg atcgacagtg ttcacaatag
     3061 gaagaagagt catccaattt tcctttaata aactgcgctt gaacgccttt atattctttt
     3121 tcaatcagcc attcttcatc aaaaaataaa tgtttatctg ttaatccgag taattttcta
     3181 gtatgattat ccattgagag actcctttgg taggatgacg ttttggtcga ctaaatttta
     3241 ccaaaagtga gtctcttttt gagttgaaac gaagaaaaag tgttagtaga aaatcgcctt
     3301 cgattttcca ccaacacaaa atattataga accctttttt tttgctatat gacaggaatg
     3361 aaaggtgttt gcaagcttaa tgtgaggaac actttccata tctgttctat actggatagg
     3421 agaagtttgg aaatatccag aaaacgaaat caatcgaggt agccacatga agaataaatg
     3481 gataaatcga agtaaaaaaa agaaagtaac acaaagatca tcagataaag tcatccgtct
     3541 ttttccatcg tataaagaag gattaacggc agatcaagtc aaggaacgaa tagaaaaagg
     3601 tgcagcaaat aattcagtcg atcccacatt caaaacaaat cagcaaatcg tattggaaaa
     3661 catatttacg tatttcaatt tgatctttct tatcttagcc attttgcttt gtttagtaga
     3721 atcctataag aacttgactt ttttaccagt tatcatagcc aatacaggaa tcgccatttt
     3781 tcaggaaatt cgatccaaaa agatcttgga cgaactaaat gttttaaata cagcaaaagt
     3841 caatgtcata cgagacggga tagaacaaac aatagatata gaagaccttg taatagatga
     3901 cattgttatt ttagagactg gatatcaaat tccagcagat gcagaggtcg tggaagggaa
     3961 actgcaagtt aatgaggctt tattgacagg cgaagcagat gaaataacca aagaaatagg
     4021 tgatccatta atgtccggca gtttcattgt ttccggaaaa gcttacgccc gattagacaa
     4081 agttggagca gattcgtata tttcccagct gacagtcaaa gccaagacaa tgggagaagg
     4141 agaacagtcg gagatgattg cttctcttaa tcaattgatc aaatggattg ggatcatcat
     4201 tattcctatc ggtatcctgc ttttttctca aagttacttt tttaatggaa acacgatcaa
     4261 agaaagtgtc gttgcaatgg aagcagcatt gatcggtatg atacctgaag gactgtactt
     4321 gctgacgacg attgcattag cattaagtgc gactaaacta gcaaagcaaa gagttctttt
     4381 acacaatatg aaaagcattg agacattggc aagggtaaat gtcctttgtg tagataaaac
     4441 aggaacgatc acagaaaata aaatgtctgt tcaaaaaatg atcgtctcta aaaaacaaga
     4501 aataatcagt cagtcagatc aattggaagc atgcattgct gactatgcaa aagcgatggc
     4561 aggagacaat gcaacaatgg aagctgttaa agcatatttt acaaaaacca ctgatcaatc
     4621 ctatcaccat gttattcctt tttcttctgt ccaaaaattc agcagtgtag cgttggcaga
     4681 caaggtgtat gttttaggag cgcccgaaat ggttttgcgc gatcaattgc cggaatatgc
     4741 ggaagagttt gtttctttcg ctgaaaaagg ctatcgcgtg ttggtctttg gtgtgtacga
     4801 tgggattctt gaagaaccag tattgacaga agcagtcaca ccattaggct atattctgat
     4861 tgctaatcct atcagaaaag aagcaagagc cacatttgat tacttcaaaa aacagcaagt
     4921 agccatcaaa gtgatttctg gagataatcc gctcaccgta tccaatgtag ctgtccaagc
     4981 agggatttca catgctgatc agtacgttga tgcatctaaa ttatcagaag aagaatatga
     5041 agcagctgtc gaaaaatata cagtttttgg acgcgtcaag ccagaacaaa agaaaatctt
     5101 cgttcaattg ataaagcgta acaatacagt agctatgaca ggtgatggcg tgaatgatat
     5161 tctagccatg aaagaagcag actgcagtat agcgatggct tccggaaatg aagccgccat
     5221 gcaggcctca caagtggtct tacttgattc tgacttttca cgcatgccag aagttgttgc
     5281 agaaggacgg cgcgttgtga acaatattga gcgatcagcc agcttgtttt tagtgaagaa
     5341 tattttctcc tttttgctct cactgttttc agtgatcttt gctctgacct atcctttaga
     5401 accatcacaa atcacattga ttagtttgtt tactatcgga cttccatctt tcttgcttgc
     5461 tctagaagaa aacaaaaagc gaatcagagg gaaattcatc atgaatgtga tggaaaaagc
     5521 tgtgccaggt ggactaacag atatgatcgt agtcggtgcg ttggtgatct gcggggtgac
     5581 tttgaatcta aataaaacag atgtttctac agcatctacc atgcttttga ttgcagtagg
     5641 gttcctcgtt ctctataaaa tctgttcacc gctgaataaa ttcagaagtc agatcattct
     5701 tttctgtgct agcggtaact ttttttcggt catctttttg cataagctgt tttcaatcac
     5761 cggtatctca gctgtatcac ttttgctgtt gagcattcta tttttctcgg cagattcgat
     5821 ttttaggcat ttgaccacat tggtagaata tttgtttgaa cgtaaaaatg aaaaagagca
     5881 aaagccgttt agtcgcgtag ctaaaaaatt tctatcgata tttaaacgag aaaattgacg
     5941 aaaagtatct caaaaaagag taaaaaagaa caaccactcg tgtatctttc gaagcaggat
     6001 gagtcatcca actttaaaac gcacgagcag ttgctctttt ttacattttc ggaatggtaa
     6061 cggtgaaagt cgaacctgtt ccaagatgac tgtctacagt aatcgtacca ttaaggagtt
     6121 gcacgtagtc ttttacaatc gctaacccta atccggtccc gcctgaatgt cggcttcgcg
     6181 ccttgtctac gcggtaaaaa cgctcaaaga tccgttcctg atcctcaatg tcgataccta
     6241 taccaaagtc ttggacagaa aacgatagat catctgttgc ctgatagcgg ataataatct
     6301 cgctgtcgct ttttgaatat tggacagcat tttcaatcag atttttgata attggataaa
     6361 ggatatctgt tttagtagta aatggttcgt tctttccaat caatcgaatc gtcaaccgtt
     6421 tcttttcgat gattggctga taattttgaa cgatttgctg gaaataaggt tctgtataaa
     6481 ttgtctggat ctcgtaagaa ttctcgccat cttttgacag ctggatgatt tcccgaatca
     6541 gtttatctaa ccggatcgca tctttttgca tgatctcaag aaaagaagtc agagtttgcg
     6601 ggtcatcttt tgcaccatcc agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag
     6661 tttttaattc gtgagagacg ttgccaacga aatccttttg tagcttttct aaacgtcgca
     6721 ctttcgttaa gtcatacgcc acccctagaa tctgtccggt accttctgaa ttagtaaagt
     6781 agcgcaaact caagtctaat gtctgccgac cttctgtaag ggtgatctcc tgatggatca
     6841 gcggatgctc aggagtcact tgatgaatca gctgaatgag ttttggctct tgaatgactt
     6901 ctgtgtaccg ctgttcaggt tgatagctta ccacgtttaa atgtagctgc atctttggat
     6961 taagcagtag taaacgagaa tcagcatcat caatcaggaa tacgccaatc atcagttcgt
     7021 tcagtaacgt atataattgc tcttctgtgg tagtacacgc ccgatacatc ttactcattt
     7081 gttcgctcaa cgcattaatc gtctgataca attcttccca ttgatcgggt gtttgcatga
     7141 ttacttccgc ctgattagga ttttgcacca ttttttttag tacgggcagc acggttttca
     7201 atggctggtt tttttgatag atcagataat agatcattcc tgcaagaaca aggaagaaag
     7261 acagaaagaa gaaaaagacc caacggcgaa aactctcagt tctcggtaaa aaaacgcttg
     7321 tcggttctgc gatacgtaaa atggcttcta actggccact ttttttgact ggtaatgcga
     7381 catataaaag atcctcattc aaagtagtac ttttgcgcaa agcgctacct aggttcccac
     7441 cattcaatac ggctttgatt tcgggacggg agtttcgctg ttcattcaat gtttcattgt
     7501 ttgtgtcaaa taagatcttt ccagtagcat ccattaaagt aatccgttcg ttcgactgat
     7561 ggacgaattc ttccaaagca gttttgtttt gcgctgcttg gaggttttcg acatctaatt
     7621 gccggatcag cagttgtgct ttttttgtca ggtagctttc ttgctgttcc acgatctgct
     7681 gttgaaaata atgggaaatc aattgccagc caccaaaaaa caaactaact aaaagaataa
     7741 gaaagaagag acgttcataa atttttttcc atttcatcgt ttgggttcct gaaacttata
     7801 gccaaatcct ctaatagtca ccaagtactt tgggtgtttt ggatctcgct cgattttttc
     7861 tcgcaaatga ctaacgtgta catctacgat tcggctttgt cctgcgaagt caaaattcca
     7921 aatgcggtct agcaatgtgt ctcggtcgat cacacggtct tttcgtttca tgaaataaac
     7981 aagtaattca aattctttcg gtgttagttc aatcagatgg tcgtctacgg tcacttgata
     8041 attggtcaaa tcagcagtga tttgtccaat cgatagatat tcaggctcag cttcatcttt
     8101 tgattgctta cgaggttcaa ttcgtctgaa tattgctttc atacgggcta atacttcgcg
     8161 aggactaaat ggtttggtca aatagtcgtc agcacctatt tctaggccaa tgatccgatc
     8221 cacttggtca tctttagccg tcagcatcaa aatcggtgtt tcattttttt cttgacgcag
     8281 ccgctgagtg atctccatgc catccatcgt tggaagcatc acatctaaaa tgatgaaatc
     8341 aaatgattgt tctaatgcta attctaatcc ttcgcctcca tctgtggcac tggttacctt
     8401 gtagccttct ttttccaaat tgaacgtcaa gagcgttaca atagaaggtt catcatcaac
     8461 aactagtatt tttttcattc aaatctcctt taaataggtt tcattctgtt tatattttag
     8521 catagaatca gtacaaaggg ctattttaca aaagaaataa atggtataat aggaaggcac
     8581 tttgctgaat agaaaggaag agcagtcaat gattggaatc agtgcatgtt taggcggtct
     8641 ggcctgtcgc tatgatggac agaaaaaaga agtaccagaa ttgatggaac tagttgaaac
     8701 tggagaagct gtcatggtct gtccagaagt gatcggcgga ttaccaattc ctagagatcc
     8761 cgctgaaatc attggtggaa atggattcga tgtgtggaaa gatcaagcaa aagtatggac
     8821 gacaagcggc gaagatgtaa cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa
     8881 attgcaggaa aaacacattg atctagtcat catgaaggaa aaaagccctt catgcggaac
     8941 ccattcgatt tatgacggaa gtttttccgg aataaaaaaa aatggtgccg gagtggctac
     9001 tgcttatttt attcagcaaa aaatgactgt gctctctgat gaagaatggc tgaaaactag
     9061 aggtgagcag aatggaaatc gaaaagacca atcgaatgaa cgcgctcttt gaattttatt
     9121 cgaccttatt gacagaaaaa caaatgaact acatggaatt atattatgca gatgattttt
     9181 cattaggtga gatcgcggaa gaatacgaag taagccgaca agcagtatat gacaatatca
     9241 agcggacaag caagatcttg gaggattatg aaaaaaaact ccatcttttt tctaattata
     9301 ttgttcgaga acaggtattg gaaaatatga ctgcatatat tacagataag tacccggaag
     9361 ataaaaaact acttgaatat gtacagcaaa ttcaagcaat cgaagaataa ataaaaggaa
     9421 tgaagaaaat atggcatttg aaagcttaac tgaacgtctc cagcaggcga tgggaaaaat
     9481 aagaaaaaaa ggaaaagtat cagaagctga cgtaaaagaa atgatgcggg aaatccgcct
     9541 tgccttacta gaagccgatg ttaacttgca agtagtcaaa gattttacta agcgcgtacg
     9601 cgaacgtgcg gtaggggcag aagtgctgga aagtttatca cctgcacaac aaatcgtaaa
     9661 aatcgttgat gaagaattga cgatcacact tggttctgaa acagcagagt taaataaatc
     9721 accaaaaata ccaactgtta tcatgatggc tggtcttcaa ggggcaggta aaacgacctt
     9781 tactggtaaa ttagccaatt atttaaagaa aaatgaaaat gctcgtccgc tcctaatcgc
     9841 aggggacgta tatcgaccag ctgcgatcga ccaattgaaa gtattaggtc aacagctaga
     9901 cgttcctgta tttgatatgg gaactgaagt cagcccggta gagatcgtcc gtcaagggat
     9961 ggagttagca aaagaaaaga aaaacgacta tgtattgatc gatacggcag gacgtctgca
    10021 tatcgatgaa acattgatgg atgagttgaa acaaatcaaa gaattgacgc aaccaaatga
    10081 gattttgctt gttgtcgatg ccatgactgg tcaagatgcc gtcaacgtag ctgatagctt
    10141 caaccagcaa ttaggtatta caggggtcgt catcactaaa cttgatgggg atactcgagg
    10201 cggggcagca ctatcgatcc gctcagtgac aggcgcacca atcaaattca tcggttctgg
    10261 tgaaaaattg actgacttag aagtattcca cccagatcgt atggctagcc gtatccttgg
    10321 tatgggggat atgctgacat tgattgaaaa agcgcagcaa gactacgacg agaaaaaagc
    10381 ggaagaactt gcacagaaaa tgcgggaaaa ttcattcgat ttcaatgact ttattgaaca
    10441 gcttgatcaa gtgatgggta tgggcccact ggaagatcta atcaaaatga tcccaggaat
    10501 gaatcaagtc cctggaatcg aaaacgtcaa ggtagatcca aaagatgttg aacgcaaaaa
    10561 agcgatggtt tattctatga cgccagcaga aagagcaaat cctgatctgt tgaacccaag
    10621 ccgtcgtcgc cgaattgctg ccggttctgg aaacagtgtt gttgaagtga atcgaatgat
    10681 caaacaattc aaggaatcaa gaaaaatgat gcagcaaatg tccaaagggg atatgaacat
    10741 cccaggtatg gaccaaatgt ttggttcagg cgtcaaagga aaactaggca aaatggcgat
    10801 gaatcgaatg atcaagaaaa ataagaagaa aaagaaaaaa cggaaataag atgaaaaaaa
    10861 cagcttcggt acagactgaa aatgtctact gccggagctt ttctatcagc aatttttatt
    10921 ctttactaat acaattactc taaacgaaga ggtggcacag atgagatgtc cgtggagtga
    10981 acaaacagaa agtatgcggc aatatcatga tactgtctgg ggtgttcctg aatatgatga
    11041 ccaaaagctg ttccgaaaat tgatgcttga tattaatcaa gccggattga gctggcagac
    11101 gattttaaat aaaagtgaag cattcgatga agcatatgat cgattcgata tcaaaaaagt
    11161 cgcaaattat ggagaaacaa aaatcagtga actaatggaa aacaaaggaa ttattcgtaa
    11221 ccgaagaaaa atcgaagcag ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga
    11281 aatcggcagt ttttcagagt atctttggtc gttttctaat ggaaaaatcg ttgattcttc
    11341 ttatcattca caagaagaag tcccaacaac aagtcctctt tcagatgaat tagcaaaaga
    11401 tctaaagaaa agaggattta aatttatcgg aagcacaaca atctatgctt ttttagaagc
    11461 tgtcgggatc atcaatgatc accttgacag ttgtttccgc aaagaagagg tcgtacgtat
    11521 cggcagaaag ggcgaataaa gcatgcgaaa aatcatattt tatggtgcta taagtttaga
    11581 tggctatttg gcaacgaaaa cggatgatct tcaatggctt tttgacactc caacaggtaa
    11641 gcaaactact tatgaagctt tttatgaaac aatcgatacg accatcatgg gcagaaaaac
    11701 ttatcaggaa gcaaaaaaat atttggagac cgaagcaatt tatccagaga aaaagaattt
    11761 cgttttttcc acccacgcac aattacaatt gccagatgct gaggtcgtaa acgaagatcc
    11821 tgttgctttt ctaaaaaatc tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg
    11881 cggtcgattg atcaaaccgc tattggaaca tcaaatgatt gatgaatggt atatccagat
    11941 tacccctgta ttattagggg acgggattcc tttattccaa aaaggaacat atgaagcgcg
    12001 ttttgaaatg ctagacacga cacgattcgg tgaatttatc gaattgcatt atgtacgaaa
    12061 gtagaagtaa ataaaataaa aaaggcaact atatgcagaa atgtcctgta catagttgcc
    12121 ttttttatta ccgttatgct ttttactctg ttaatttgtt agaaaaatct agatacgcat
    12181 ctaaaatcgt ttggaagaaa cttaatgttc cttcaacgat attaccattt tcatcaatta
    12241 gattcgcaac gtttccgata tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga
    12301 caagtgattg gcgtagatgg tggtttgcgc caaagccgcc gatcccacct ggtgatgcag
    12361 taacgactaa acctggttta tgatcccaaa cactgtgtcc ataaggtctt gagcctacat
    12421 ctaaagcatt tttcaatact gctggcacag aacggttgta ttcaggagta acgaaaacga
    12481 caccgtctag tcccctcact tcctcacgaa aacgagtcca ttcagcagga gcctgttctg
    12541 gagtttcgag atcctcattg taaaatggca gatcatcgat ttttacaaat acactttcgt
    12601 acccttcagg aagcaatttc gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc
    12661 ttaaactacc aataaaaaat ccaatttttt ttgtcatacg atttttcctc cttcaaacta
    12721 tctataataa tcttacaaaa gataagtaac tggagcaaat aaaaacactt actgacaaaa
    12781 gctacataga agatagatta cttattttta acaaaaaaga agtctaaaat cttctatttt
    12841 ctaaaaaaaa tcgaatttaa tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa
    12901 actgttacac tacatcttgt gaataaaaca atggaggtgg aaataaataa tggcagttaa
    12961 aatccgttta aaacgtatgg gttctaaaaa gagtccgttt taccgtatcg tcgtagctga
    13021 ttcacgttct cctcgtgatg gacgtttcat cgaaactgta ggtacttaca atcctttgaa
    13081 agaccctgca gaagtagttt taaaagaaga cttagttctt gactggttat caaaaggtgc
    13141 acaaccttct gacactgtac gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca
    13201 cgaagctaaa ttctcaaaga aataaggtga ccggatatga aagatttaag cgacttagtc
    13261 ttaacaatcg ttcgtccgct agtcacttat cctgatcaag tcaagttaga cgtcgtagag
    13321 tcgaaagact tttacgagta caatctaaca gtggctcctg aagatattgg ccgtatcatt
    13381 ggaaaacaag ggcgtgttgc taaagcaatc cgcacaatcg tttacggtgt tcggatcaat
    13441 gcaccgaaaa aagtgcgttt gaatatcatc gatgataagg aaaagtaaat aaacgtgctg
    13501 tactcgaatt ttcgagtgca gcttttttgt tttttttatt aattgaagaa atgattattt
    13561 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13621 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13681 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13741 tagtaccaaa acggcttcca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13801 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13861 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13921 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13981 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    14041 gcagccattt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    14101 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    14161 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    14221 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    14281 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14341 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14401 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14461 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14521 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14581 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14641 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgcta acactattcc
    14701 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14761 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14821 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14881 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14941 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    15001 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    15061 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    15121 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    15181 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    15241 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15301 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15361 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15421 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15481 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15541 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15601 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15661 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15721 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15781 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15841 aagtgacttt ttctaaaatc ttctttacag taggttgata atatgttagt attttatggt
    15901 gagtgcaaaa gcacttaact attacgatat tccgctgtga gaaaagctca taagaatatt
    15961 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    16021 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    16081 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    16141 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    16201 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    16261 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16321 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16381 cttagcatgt aatttgaaca ccctgatcca gttttttctt taattattgg taggggagaa
    16441 taatggatta gaataaatct acctataagt agtagtaaaa aaaagagcac tatgataata
    16501 tctttcttaa gagtaaccgc tttaataaaa aatccagaat attctaacat aaatggagta
    16561 ttcttagaat caaaaggaga aagaagatgg tttatactta tacggagcaa gaaaagatga
    16621 aagttttaga cacattcatg aatgatggaa aagtaaaaca agtaccaagt tcaacaaaaa
    16681 agaaatacat cctattgaaa gaagtcttga aacgctttga ccatggagtg acttatacag
    16741 aaacagaggt taattcgata ctgctgaacg tattttctag tggcgattat gtcgaacaaa
    16801 gacgttatct aatcacattt ggctttttca aaagaagttc agacggtcgg gcatatcaga
    16861 tgatgggaat tgaaaattag aagttttaat ttatactcat tgaaaaaagt tcaagtatgc
    16921 gatattggtt ctacaccgca tacttgaact ttttttgtca tctgtatcta taatacaaag
    16981 ccaagaacgt tattcaaaat atggatagct atgataaaag ctaatgagcg attcgcagtt
    17041 aaataagcaa tcgtcagtaa aatcgttcca ggagcgtaca tgaaaaaatc aattggatgc
    17101 gttacatgaa taaagataat tctcttttat ttgtacgaat aaataccaaa acgtacctcc
    17161 taaaattaga ttttggacag atgcccttta aaaagataac aagatttcgt tcttgaaaca
    17221 atatatatgc ttttattcca atgaggttgt gaaaacagta ttttgtcctg agcgttagaa
    17281 aattgaaaat ggggaatagc aactcgtact ttaccatttt ttaagtgaat acaagatcag
    17341 ctaattgaac a
//
LOCUS       DACDHL010000050        17130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDHL010000050 DACDHL010000000
VERSION     DACDHL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.050"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            126..1268
                     /locus_tag="IV228_001772"
     CDS             126..1268
                     /locus_tag="IV228_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8717664.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            1284..2495
                     /gene="thiI"
                     /locus_tag="IV228_001773"
     CDS             1284..2495
                     /gene="thiI"
                     /locus_tag="IV228_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8717665.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            3068..3715
                     /locus_tag="IV228_001774"
     CDS             3068..3715
                     /locus_tag="IV228_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8717666.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            4108..6753
                     /locus_tag="IV228_001775"
     CDS             4108..6753
                     /locus_tag="IV228_001775"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8717667.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            7063..8376
                     /locus_tag="IV228_001776"
     CDS             7063..8376
                     /locus_tag="IV228_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8717668.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            8366..9019
                     /locus_tag="IV228_001777"
     CDS             8366..9019
                     /locus_tag="IV228_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8717669.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            9074..9766
                     /locus_tag="IV228_001778"
     CDS             9074..9766
                     /locus_tag="IV228_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8717670.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            9916..10116
                     /locus_tag="IV228_001779"
     CDS             9916..10116
                     /locus_tag="IV228_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8717671.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(10370..12049)
                     /locus_tag="IV228_001780"
     CDS             complement(10370..12049)
                     /locus_tag="IV228_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8717672.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(12051..12263)
                     /locus_tag="IV228_001781"
     CDS             complement(12051..12263)
                     /locus_tag="IV228_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8717673.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            12656..14386
                     /gene="efrA"
                     /locus_tag="IV228_001782"
     CDS             12656..14386
                     /gene="efrA"
                     /locus_tag="IV228_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8717674.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            14383..16143
                     /gene="efrB"
                     /locus_tag="IV228_001783"
     CDS             14383..16143
                     /gene="efrB"
                     /locus_tag="IV228_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8717675.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            16238..16435
                     /locus_tag="IV228_001784"
     CDS             16238..16435
                     /locus_tag="IV228_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717676.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            16588..17073
                     /locus_tag="IV228_001785"
     CDS             16588..17073
                     /locus_tag="IV228_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8717677.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         5525 a         2945 c         3688 g         4972 t
ORIGIN      
        1 attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg aaacaagtga aaatttcacc
       61 tgctcttgaa agcaccttgg acaaccgtta taatagaaaa gaatttcaaa aaagaggggt
      121 ttcttatgat atattttgat aacagtgcaa caaccccaat ctttccgcaa agcttagatg
      181 catatgtaaa aaccagtcag cggattattg ggaatccatc aagcttgcat gatttaggca
      241 atcaagctaa ccggttgttg cagcaagcaa gaaagcagat cgctgatttg atccatgtag
      301 aaccagaaga aatctatttt acttcaggcg gaacagaagg agataactgg gtattaaaag
      361 gaacgatgat cgaaaaaaga gaatatggga atcatatgat catttcagga gtcgaacatc
      421 cggccgtcag cgagacagca gaacaattaa aagaattagg aatcgaagta agtatcgccc
      481 cagtggataa acgcggattt gtacgtgtcg acgaattgaa agaattaata agaaaagaga
      541 cagtattagt ctctgttatg gctgtgaata atgaagtagg atcgattcag cccatccagg
      601 aaatcagtga tcttttggat gattttccta aaattcattt ccatgtagat gctgttcagg
      661 cgattggaaa agtaccattc tcgcaatggt tgacagaaag agttgatttt gcaacatttt
      721 cagctcataa attccatggc ccacggggaa ctggtttcat ttattggaaa aagggtcgtc
      781 gtttagcacc attactgaca ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa
      841 atgttccagg tattgtggct atggttaaag ctttacgttt atcattagag ataaaggaac
      901 aaaaacctga gcagacacaa cgcttaaaag tagctctaat caatgcgttg aagacgtatg
      961 agaaagtgac gattttttca gaaggaactg actatgcacc gcatatttta tgttttgctc
     1021 taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga agaaaaacaa atatttgttt
     1081 ctactactag tgcctgctcc tcacgtaaac atatggcaag cagtacattg cacgcaatgc
     1141 atgtaccaaa tgatctggct acatctgctg ttcgaatcag cttagatgaa aacaattcgc
     1201 ttgcagaagt cgagcaattt atgattattt tcaagcagct ttataagaaa ttttctgcaa
     1261 tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat tatggtccgt tatggggaac
     1321 tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca actagcacaa aatgtcagac
     1381 aagctttgaa agactttcca gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt
     1441 tattgaatgg cgcagacggg cagctagtta ttccaaaact agcaaaaata tttgggatcc
     1501 aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga agttttgaaa caagctgttc
     1561 aagacatcat gaaagaaatt tatactggga aagaaacatt taaaattatc gctaaaagaa
     1621 gcgatcacca atttgaattg acaagtaatg aattgaatca aacattaggt aatgctgttt
     1681 ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc agatattcca ttacgtgtag
     1741 agattcggcg tgatggcgct tatctatcgt atgaaacgat caagggagct ggaggattgc
     1801 ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg ggggattgat tctcctgtag
     1861 cagggtactt tgccatgaag cggggagtgg aaattgaagc tgttcatttt gctagtccgc
     1921 cgtatacaag tgaacaagca ctgcaaaaag caaaagatct aacagccaaa ctagcacctt
     1981 atgtgggaac gatccatttt atcgaagtgc catttaccga gattcaagaa gaaatcaaaa
     2041 agtcagtgcc acaaggatat tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg
     2101 atgaaattcg ccgtattcgt catggtttag tcattttgaa tggtgaatca ttaggtcaag
     2161 tggcttcaca aactttgcaa agtatggtgg ctatcaatga agtaacgaat acgccaatca
     2221 ttcggcctgt agcgacaatg gataaacttg aaatcattga agtagcagaa cagatcgata
     2281 catttaatct ttctatccag ccttttgaag attgctgtac gatttttgct cctccacagc
     2341 caaaaacacg ccctcaactg gaaaaggtcc atcaatatga agagcgttta gcaatagatg
     2401 aaatgatcca acgagcacta gctggcttga agatggaaga gatacaagca gaatcgtcta
     2461 atgaaaaaac tgaagaattt tcagaattgc tataagaggt tatgaaaata gtgttttgac
     2521 cagagtatta gagaaacaaa acgaaaaaat agctccatgt attttaccgt ttttgaacta
     2581 atgtcgaaga ttagctgatt gaataccatt tatcaagaag ttatggaaaa gccggttctt
     2641 ttttactaac tgaaaaataa aattttattt agagatcaag ctacagttga taaatgatat
     2701 aaaaaagaga atttcccatg aaatagcgag gttgtgacag aagtgggcag cttcaagaaa
     2761 taagaaggaa tttacgaaaa ttgcttttca aatttttgtg aaatttcggc ttatttttcg
     2821 aagaagttac ttctggaaca ccgtgtatca gaggttgcga aaaagctgcc ctgcatcaag
     2881 taataagaac agccaaacaa aaatagttcc tcatatttta cgtttggttg agcttattac
     2941 cgcagatgca agcttttgaa caccgtttta tttgtctcac acccttttcc ttcctacttg
     3001 taaccttgct cccgtgttag aaacatgtta cactaaatat gtgaaattat taacaaggag
     3061 cgtcgttatg cgagaaaaaa cagatacact gccaaaagca actgcgaaaa gattaccttt
     3121 atatttacgc tacttgaaga tgctggatga ctcaggtata agtagaatca aatcaaatga
     3181 gttcagcgaa atcacccaaa ttccttctgc aacgatccgc agagacttct cgcaattagg
     3241 cgagctaggc agaagcggat atggctatga tgtccctttt cttatcgatg tattcaataa
     3301 tatccttaat actaaagaag aaaaacgaat cgcattagta ggatatggaa atcttggcaa
     3361 agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga
     3421 taatgatcca gcactgatca atcgagtaat tgatggagaa atgatctatc cgattgatcg
     3481 tttcgcggag atagccaaag caaaaaatgt gacagtagca atctctacgg taccaagtaa
     3541 atattcacaa tctgctattg atgaaattgt caaaggaaat gtaaccgcta tacttaattt
     3601 tgcgcctgac agagtaactg tcccagctta tgtcaatgtt caatacattg atttgacaac
     3661 cgagttgcag acgcttattt actttgatga gaattactca gaagtttttt cttaggaaaa
     3721 tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat tctttcaaaa attttttagt
     3781 actaaatgaa gtggtttttt cacgaccact tgttgactaa aaaaaaaaac cattgtagaa
     3841 tgaagcataa cgctgaacaa gaggagtaac tggcacattc attcttagag agagaactat
     3901 ctgctgaaag gttctcaatg acaaccagtg aaggtagctt gggagtttct gtcttgaatg
     3961 gagtagagat agacgagtcg tgatcgttaa ctcacttgag aggcaataat gattgattat
     4021 tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa tacgaaagta ttttaggacg
     4081 ttttttagtt taaaggagga aaacagcatg acggaagaaa ataacctatc aacaaaatat
     4141 aatccgcaag aagttgaagc tggacgttat cagcattggt tagaacaaga tttgtttaaa
     4201 ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa tcccgccccc aaatgttact
     4261 ggaaaacttc atttagggca tgcttgggat acgaccttgc aagatatgct gattcgtcaa
     4321 aagagaatgc aaggatatga tactttatgg ctgcctggta tggaccatgc aggaatcgcg
     4381 acacaagcaa aagtagaagc gaagcttaga gagcaaggga tttctcgtta tgatctaggt
     4441 cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag agtatgcgag ccacatccgc
     4501 gaacagtggg ctaaaatggg cttatcttta gattacagta gggaacgttt tacattagat
     4561 gatggattat ctgaagcagt acgtaaagtt tttgttacct tatacgaaaa aggtttgatt
     4621 tatcgtggtg aatatatcat caactgggat cctcaagcac gaacagctct atctgatatc
     4681 gaagttattc ataaagatat cgaaggcgcc ttttatcata tgagctatga actagcagat
     4741 ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa cgatgttggg agatacggcg
     4801 attgctgttc atccagaaga cgagcgttat caagcgctca tcggcaaaaa agtgattcta
     4861 cctttagtca ataaacaaat cccaatcatt gcagatgaat atgtagatat ggaattcggg
     4921 acaggtgtag taaaaatcac tccagcacat gatccaaacg actttgaggt aggtaatcgt
     4981 catgatcttc cacgcgtaaa tgttatggat gatgatggta cgatgaatga actagcagga
     5041 aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa
     5101 atcggtcgat tgattaaaat cgatacgatg atccacagtg tgggtcattc cgagcggaca
     5161 ggtgtcgttg ttgaacctcg gctgtctact caatggtttg taaaaatggc gccgttagcg
     5221 aaaaaagcca ttgataacca agaaacagat caagcagtgg aattcttccc tccaagattc
     5281 aatcaaacgt ttttacgttg gatggaaaat gtccatgatt gggtaatctc acgacaatta
     5341 tggtggggac atcaaattcc tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg
     5401 atggaagcac cagcggatag tgaaaactgg gtacaagatc ctgatgtttt agacacttgg
     5461 tttagttcag ctttgtggcc tttctcaaca atgggctggc cagatacgga aagcgaagat
     5521 tataagcgat acttccctac tagcacttta gtaaccggtt atgacatcat tttcttctgg
     5581 gtaagcagaa tgattttcca aagcttggaa tttactggcg aacgtccatt tgagaatgtc
     5641 ttgattcatg gattgattcg tgacgaacaa gggcggaaga tgagtaaatc attaggtaat
     5701 ggaatcgatc cgatggaagt aatcgaaaag tatggagcag atgctctccg ctggttctta
     5761 tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct atgaaaaaat ggatgcttct
     5821 tggaacttta tcaacaaaat ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga
     5881 atgaccgtag aagagatcga tttatctgga gaaaaatcag tggctgaccg gtggatcctt
     5941 actcgtttga atgaaacgat cgaacgagta actgagctat ttgatcgttt tgaatttggt
     6001 gaagcaggtc gacaactgta taactttatc tgggatgatt tttgtgactg gtacatcgaa
     6061 atgagtaaag aagtacttta tggagatgat gctgtttcaa aacaaaccac ccgtagcatc
     6121 ttagtccata cacttgatca gatcctacgt ttattgcatc caatcatgcc attcgttaca
     6181 gaagaaatat gggaacatat tcctcatcag ggaaattctc tagtagtggc agaatatcct
     6241 gttgtacgtc cagagtttga tgatgaaaca gcttctaaag ggatggaagt attgaaagaa
     6301 ttgattcgag ccgttcgaaa tattcgttca gaagtgaata caccattgtc taaaccaatt
     6361 acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat ttttagttga aaatacaagc
     6421 tatatcgaac gcttctgtaa tcccgaagag ttgacgatat caagtgagat cactgcgcca
     6481 gatctagcga tgtcagctgt tctgacaggt gcagaaatct tcttgccgtt agcaggattg
     6541 atcaatattg aagaagaaat caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa
     6601 gtaaaacgtg tccaaggaaa actttctaat gaacgttttg tttctaacgc gccggaagaa
     6661 gtagtagagg cagagcgtgc aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa
     6721 gaacgaatcg aaagtttgcg tactattcaa taagaataga aaaagagagt gtgaaatcag
     6781 ctagctatta ctaaaagcta gctgattgaa catcgttaca ggaaagagtt tgagcctcac
     6841 tcttagcttc gattcataag gagatgtgac ataaattttg tcgcatctct tttttagtct
     6901 actcttatta tgtaggagaa ggagggaggt ggatcatgta tcaagagtca ataaagtagt
     6961 tgataaaatc gttcctctct ctgtttcttt tctctttgtc atacttttat ttttcttatg
     7021 tgttctattt gttataataa aagagaaatg ggggaattgt tgatgacgat tgaagaagca
     7081 atagaatgga tccatagtcg attaccattt ggttcacgtc ctggccttga ccgagtcgaa
     7141 gctttattag aagtggtagg gcatccagag agaaaagtaa aaacgatcca tatcgctgga
     7201 acaaacggaa aaggttctac tgtagcttat ttgagaagcc tattagaaga ggctgggctg
     7261 acagtaggta catttacttc accttatatc gaaacgttca atgaacggat cgcaatcaat
     7321 ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa aaaaatatca gccactgatc
     7381 aaacaacttg atcaatttga agcagtagct ggtattactg aattcgagac attaacagca
     7441 atggcatttg attatttttt gatgaagaaa gtggacgtag cgattatcga agttggctta
     7501 ggcggcttat tggattctac caatgtagct gtaccaatgc tgacgggaat aacaacgatt
     7561 ggacttgatc ataccgaaat acttggagat acaattgaag aaatcgctgc acaaaaagct
     7621 ggaatcatca aacaaggcgt tccagtagta acaggaaata ttgccccaga agcttttact
     7681 gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat
     7741 aaaataaact atctgcatcc tgatgagatg tggggagaag tatttgaatt ttttaatgaa
     7801 ataggaaaga tccctcaatt aaccactccg cttttagggc aacatcaagc tgaaaatgca
     7861 ggagtagcca tcgaattgta ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa
     7921 aaagatgttt tgaagggatt gaagaaatct cgatggccag gaagaatgga gcgtttgagt
     7981 aacgagccat tagttgtttt agatggtgcc cataatacgc atgcaatgaa ccggcttgtt
     8041 gagaatttga aaaaagaatt caaaggatat caaatcaaca ttttgttttc cgccttacga
     8101 actaaaaatg ttagagaaat gctagaacga ttgcagcaag taccaaatgt ccatatttat
     8161 ttgacgacct ttgaacatcc aaaagccatc gaattaagtg aagtatcaga tttagcaagt
     8221 gagagagtat caatcgtttc attgtggcaa ttcggattag cagatatatt ggaaaagatg
     8281 acaaatgaag acgtattagt tgtgaccggt tcactttatt tcatttcaga agttcgccat
     8341 ttattaatac aattaggagg agatcatgaa gtataatgca gtgatttttg acatggatgg
     8401 tttgctgttc gacacagagt tagtgtatta cgaagccaca caagtagtag cagacaagat
     8461 gggttttcct tatgataaag acctgtattt aaaattctta ggtgtttcag atgaagaagt
     8521 atgggcgaat tatcatatga ttttctcgga atttggcaaa acaagtgtac aaagattcat
     8581 agatgattca taccaggaaa cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc
     8641 cggtgtgttg gaactcctct cttttttaga agaacatcag attcccaaag ttgtcgcttc
     8701 cagtaaccaa cgagcagtca ttgaactttt attgaagaag aacaatctgt ttgaacgatt
     8761 tgagacgatt gtttctgcag aaaatgttca acgagcaaaa ccagacccag aaattttctt
     8821 gttagctaat cagaagctag gtacagcgaa gaaagaaaca ttgattttag aagattcaca
     8881 aaatggtatt ttagcagctt ctgcggctga gatcccggta gtcatggtac cagatctgct
     8941 tccaccatcc caagaattga tcaaaaaaac agctggggtt gtttcttctt tacatgaaat
     9001 ccctgcattg ttaaaataaa aatccatctc ttttcgtatc ttattttaga taagatagaa
     9061 aggagatgtt tttttgggaa aacgattgat caaagaagta cctactagct ctttaccaag
     9121 ggaacgtatg gaaatttatg gcgcggaagc tttatcggat caggagcttt tggcaatctt
     9181 actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt gctggaaatc tgttaaaaac
     9241 gtttggagga ctggcctctt tacgacaggc tactttgcat gagttagaag agattcgagg
     9301 gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc gaattaggtc gacgaattca
     9361 gacggatcat gaaagagcgg ctccaccggt tcgtaccagc tacgaattgg cccagcaatt
     9421 gattctggaa atgaaagatc acaaacaaga acatttggtt tgtatttatt tggacacgaa
     9481 gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg ctgaatcagt cagtcgctca
     9541 tcctagagaa attttccatt atgctgtacg ttattgtgca gcaaggatcg ttttagcgca
     9601 taatcatcct tctggtaatg ttattccatc gcagcaggat atgaatttta ccaaaaggat
     9661 acaaaagtgt ggagaaatga tggggatcac tgtactggac cacttgatca tcggacgcaa
     9721 acgatatttt agtttgagag aagaaggaat gatggaagag aagtaagttg ctttcttgca
     9781 aaaaaaatga agcaagtgta caattatatt gtaattttgt ttgggaatag aagttgaaac
     9841 atctgttgca cctctatcgt acacgagcaa tgttacaatt taaattagtg cttttggcac
     9901 gaggaggata ttattatgga aaacggaaca gtaaaatggt tcaacgctga aaaaggattt
     9961 ggatttatct cacgtgaaga tggaagcgac gtattcgttc acttctcagc tatccaaggt
    10021 gacggattca aaacattaga agaaggtcaa gcagtgactt tcgacgttga agaatcagac
    10081 cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt atctgtaaat caaattgatg
    10141 gtagacatca atttttacga gagaacaaag cgagtaacaa aaatacctgc cacaagagac
    10201 aatttctctt gtggcaggta tttttttgta tgaaaaagta aataaattta agaggctatg
    10261 aaagagccac cttgtatcaa ggaataagaa caaacaaaaa tggcttctca tgtttttcat
    10321 ttgttttctc ttatattcag gacaaggtgc tgatttcata acctcttatt tattcttcat
    10381 ctggtgtcat taccattggc aaaatcattg gatgtctttc tgtacgttcg aataagaaag
    10441 gctgaagcgc gccagtcatc gcttctttca atttatattc tgaacagtca ggtccattca
    10501 aacattctct taaagcgtgg aataagattc gctgagcttc attgatcatt tctcctgatt
    10561 ctcgcatgta gacaaatcca cgagagagaa tatctggacc agccaagatt tcttttttct
    10621 tgatatccac tgtcgcgaca gctaatacaa gaccttcttc tgaaagcagg tgacggtcac
    10681 gtaaaacgac attaccaata tcaccgatcc cgctaccatc gatatacaca tctccagcca
    10741 caaaatgccc agcaggacgc gcagaatcgc tagttaacgc taaaacatcg ccattttcca
    10801 tgatgaagca gttttctgca ggaacaccta catcttgtgc tagagaagca tgtactttca
    10861 acatgcggaa ttctccgtgg acaggaataa aatattttgg cttcatcaaa cgaagcatta
    10921 atttttgttc ttcttgtcca ccgtgaccgg aagtatggat gttattgatt tttccgtgaa
    10981 tcacttctgc accagcttca gacagcaaat tgatcaaatg attgacactt gttgtattac
    11041 caggaatagg tgaacttgaa aagatcactg tatcgtctgg ctggatttgg atttgacggt
    11101 gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc tccttgagaa cctgtacata
    11161 ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg tgcatctaca aatgtgccgt
    11221 ctggaacttt gatatatcct aaacgttgac cgttgacgat ggcattttcc atgcttcgtc
    11281 cgaaaacagc gattttacga ccagttgcca cagcggcatc tgctgcttgc tgcaaacgga
    11341 agatgttaga agcaaagctg gcaaaaatga tacgtccttc gatttttgaa aagatcttca
    11401 aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt tggaatctca gcatttgtac
    11461 tatcagataa aaggcacaag actccttctt ccccgatacg agccatttta tgcaagtttg
    11521 ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc agtcgctacg atatttccag
    11581 aaggtgtttt caccacgatc cctaatgcat ctgggatact atgggtcgtt ctgaaaaagc
    11641 tgacagaagt ctttctaaaa cggatgatcg tatcttcatt gatctcatgg agctctgcat
    11701 ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa cgctaatgcg agaggaccag
    11761 catagatagg aatatttgct tgtttcagca agtagggtat tccgccgatg tggtcttcgt
    11821 gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt ttgtatgatg tagctgtagt
    11881 cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg gaatttgatc ccggcatcaa
    11941 tcaaaatgat ttcatcttgg aattgtaccc catacgtatt tttcccgatt tcacctaatc
    12001 caccaatagc aaagacgcct gtctcgttat ttttgatgga aagtttcata ttaaaactcc
    12061 actaatgaga aatcagcatt ttctttttcg tactctagat gacttccttc tagaagttga
    12121 acgaattcaa tattgtatgg ggtattttct tccacacgtt ggcgagcgtc tacatcgctt
    12181 tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag ggttgcgtac ttttgtttct
    12241 tgataataaa ctttatagat cattgttttg tctcctttat ctgcgtaaag cagtgattta
    12301 gctagttgat ttttttagac aacacaaaac gaggggacaa gccacctggt ttttcatatg
    12361 ctttgactag aatactggat tcgagtcaaa gagacaagcc tcgtttttgc ttatctgttc
    12421 ggatattttt tcaaccgtac cttctgtata aaaaatatac aacaattaat tgttattcta
    12481 gcataaaaca gcaaataagt atagaaatca actagattga aagaaaaaat caatgtttat
    12541 catcccgcta taatcataat gactttttta tgagagaagt gttctattaa taggctcgtt
    12601 gtgctatcat ttttagtaat gaaaatgtaa caaaagatta aaaggagatg agaaagtgaa
    12661 actaatgtgg cattatacca tgcgttataa gaaattcttg tttttcaatt tcatatgcgt
    12721 atttggtttt attttgattg aattaggact tcctacgatt ttagcacgta tgatcgatgt
    12781 aggaatccgc aatgatgatt ttgattatgt gaagcaacaa gggatattta tgatcgttat
    12841 tactgtaatc ggtattattt tgaatatctt gttaggttat tttggttcga ggattacgac
    12901 taatatcgta gcagatattc gagatgattt gttcgaaaag atccaaagtt attcgcatca
    12961 ggaatacgaa aatctaggcg tatcttcatt gatcactcgt acaacaaacg atgcttatca
    13021 gatcatgttg ttcctgcaaa atattttacg tatcggtttt atggcaccaa tgatgttcat
    13081 tgtcagtttg tacatggtca tgcgtacaag tgtgacactc agtttgtatg tagtaggtgc
    13141 attaccttta ctattattgg cagtcgtagc gattgctaag gtgtctgaac cgttatctaa
    13201 aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg cgggaaaatc tttcaggatt
    13261 gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa aaacgttttg ctgtagtcaa
    13321 tgagaattat gcttctagtt caaaaagctt attccgtttg atggctgttg cacaaccggg
    13381 tttttatttc ttatttaata tcgtaatggt attgatcatc tggagcgggg cagtgcaaat
    13441 cgatgcaggc gagcttcgtg taggagattt gattgcgttt atcgagtata tattccatgc
    13501 cttgttctca ttcatgttat ttgctagtgt gttcatgatg tatccaagag cagctgtttc
    13561 agctggacgt atccaagaag ccctagatgc caaaccttcg attgtagaag atccaaatgg
    13621 tatatctgat acaaaaacta aagggtatat cgaatttcaa aatgtaacat ttgcttatcc
    13681 aggccattcg caagagccag tgatacgaaa tgtcagtttt acagcctctc caggagagac
    13741 cgttgctttt attggaagta ccggtagcgg aaaatctacg ttgatccaat tgatccctcg
    13801 cttttatgat gtgagtgaag gaaagatctt gatcgatgga gtagacgtga gagattatcg
    13861 gctaagcaag ttgcgagata agattgggtt tattccgcaa aaagcgctgc tatttactgg
    13921 aacgattgca gataatttgc gatacgggaa agaagatgct acgcaagaag aaatggaacg
    13981 tgcagctgag attgctcaag cttcagaatt catttctcga aaaccagatg gatttaatga
    14041 acatttatca gaaggtggtt ccaatttttc aggtggacaa aaacaacgat tggcaatagc
    14101 cagagccatc atacgccgtc cggaaatcta tatttttgat gacagctttt ctgcattaga
    14161 ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa gaaacgacag aatctactgt
    14221 attgatcgta gcacaacgtg tgggaacgat catgcatgca gataagatca tcgtattaaa
    14281 tgaaggagaa gtcgtcggta tggggacgca tcgggagttg ttggaaaaat gcccgattta
    14341 ttatgatatt gccgcttctc aattatcaaa ggaggaatta gcatgaaaaa aaccttttct
    14401 tccttaaaac gtttagctcg atatatcaga ccgtatcgtg agacttttct tttagtgatc
    14461 ctatttacag ttctgacagt ggctttcaac gtggctttgc catatgttac aggacttcct
    14521 actacagaaa tcagccgtaa tattgccaat ggcgaatcaa tcaatttttc ttatatattc
    14581 aaatgtctca tttggatcac agttgttgga atagggtatt gtatttcgca attactttct
    14641 ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg atttgagaag agacatagaa
    14701 gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa accagcaagg aaatattttg
    14761 tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt tacaacaagc ttttattggt
    14821 atcgtaaatg cgatattggg aattacgttg gctgcagcaa tgatgtttta tatccagccg
    14881 atgatggcgc tgatctctat gatcatgatt ccaacatcga tctggatctc aaaacgagtg
    14941 atcaatgctt cgcagaaata ttttcaaagc atgcagaatt ctcttggtga gctaaacggc
    15001 tacgtccaag aaaatatgac aggttttagt gtattgaaag tatatggacg cgaaaaagaa
    15061 acattggaag gattcaaaaa agttaatcac agcttgaaat actttgggtt tcgtgcagca
    15121 ttcatttctg gcttgatgat gcctctagtt caattaactg cctatgctac ttatattggt
    15181 atggctgttc taggaagctt ttatgttatt actggggcga tcgttgtcgg tcaactacaa
    15241 gcattcatcc aatatatttg gcaaatcagt cagccaatgg ggaacgtgac acaattgtct
    15301 tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat tcgagatttt agatgagcca
    15361 gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa cgattttagg agatgtgaca
    15421 ttcgatcatg tcgattttgc atatgatccg aagaaaccat taatccaaga tttgaatttt
    15481 gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga ccggtgccgg caagacgaca
    15541 ttgatcaatc tattgatgcg tttttatgac gtagacaaag gagcaataag gattgatgga
    15601 atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc tatttggtat ggtcttgcaa
    15661 gatgcatggc tttatcaagg aacgattgca gataatatcc gcttcggaaa gcttgatgcg
    15721 acagattatg aagtagttga tgctgcgaaa acagcaaacg tagatcattt tatccgaacg
    15781 atgccagatg gatatgatat ggccatcaat tctgagggag acaatgtgtc tttaggacaa
    15841 aaacagttgc tgacgattgc ccgagcagtg gtctccgatc ctaaaatctt gattttagat
    15901 gaagcaacaa gttcagtaga cacccgttta gaagcgttga ttcaaaaagc aatggatcgc
    15961 gtgatggagg gacggacaag tttcgtcatc gctcaccgtt tatcgacaat ccgtgatgca
    16021 gatctgattc tagtgatgga tcaaggtcag atcattgaaa aaggaacgca tgagagttta
    16081 ttggcacaag gtggatttta tgaaaaactg tataacagcc aatttgctga ggaagcagaa
    16141 taagtcatat aagaataaaa cagtttaaaa cttatttttt gttctctatt cttgataatg
    16201 gtaaagtaaa gttatcaaga aaagtgagga cacggagatg gaacagtttt taaatgaaaa
    16261 aattactgaa gccaaaagag aagttgatcg tttaacgcac gtctgtcaga cgaagccttt
    16321 tgattcagct gatttttttg aaaatgagtt tcaaatcgaa cgcttggcag cacaaataga
    16381 agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat atagatgtac aataaaaaca
    16441 ggtaaaacgt cagttaccag atactgtaaa tagacatctg gcctgagcag gttttacctg
    16501 ttttttttct tatgaaataa atatatgcta tgataccttt attcaatagg attagtttct
    16561 acaaaaaatg aaaggagaaa aataaatatg gaacggttta tagcatcaaa tgccacagtg
    16621 attggtgatg tgacattgtc agaagatgtg acgatctggt atcaagctgt tttacgcggg
    16681 gacagtaatt ggataaaaat aggacaaaga acgaatatcc aagatggtac aatcatccat
    16741 gtcgatcatg atgcacctgt agatattgcg gagaatgtga cggtaggaca tcagtgcatg
    16801 ctacatggct gtacaatcga aaaaggggcg ctgatcggga tggggaccac gatattgaat
    16861 catgcggtta ttggtgagaa tagtttgatt ggtgctggat cattagtaac agaaggaaaa
    16921 gttattcctc ctaatgtctt ggcttttggt cgcccagcta aagtgatccg tcctttgaca
    16981 aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt atgtcgagat aggtcggatg
    17041 catgcgcaag gaaaattcaa ggagcaaaga taggcagtag catccataaa ataaaaaagg
    17101 atggtcagtt tgagttgacc atcctttttt
//
LOCUS       DACDHL010000051        16602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDHL010000051 DACDHL010000000
VERSION     DACDHL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.051"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..463
                     /locus_tag="IV228_001786"
     CDS             35..463
                     /locus_tag="IV228_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717678.1"
                     /translation="MEIENFEQKKQILSNLLIDGFDNANYSHKLLFKSELDDEKEFDK
                     QKDLMCALTYLNQAHASFTNAYTFIALNDELLGGRQEFDNILHQFTEFNTEFLNNVRT
                     NHSHQWSDIEFKRLVDFFEAASGLLNGHERIQGLINEARK"
     gene            complement(570..761)
                     /locus_tag="IV228_001787"
     CDS             complement(570..761)
                     /locus_tag="IV228_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717679.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEASQWLLKTYTNNKKLRKKYPDA
                     LLKDDQIMPEPMIFTKWDK"
     gene            complement(769..957)
                     /locus_tag="IV228_001788"
     CDS             complement(769..957)
                     /locus_tag="IV228_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717680.1"
                     /translation="MRSTDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMDQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(954..1529)
                     /locus_tag="IV228_001789"
     CDS             complement(954..1529)
                     /locus_tag="IV228_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717681.1"
                     /translation="MTKQVNFRPELKKVTSKSNGNTEVLLVVSNGSLRGSTENLTEFL
                     GSTVTVVIQPETIEYTVPVNKQTKKPNVEYVVNADGTIDMLKEEQTSLDVGDGVEEVE
                     NVKILVSKETIDEFIKTATTLQLPENVTVNIRDVLIRLAEGDSMSEIAADHELSEIAL
                     IDQIELARHYFAPYADSWSKHKDDIIFPEEQ"
     gene            complement(1531..1986)
                     /locus_tag="IV228_001790"
     CDS             complement(1531..1986)
                     /locus_tag="IV228_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8717682.1"
                     /translation="MATRSKYGNKKHEVDGITFDSKAEARYYMKLKRNGMSFMPLSET
                     YCAMQENVLLQEGYLCNDRKIAPIYYRADFVIYENGQVKKVIDVKGYQDAISMLKMKM
                     FAHRYGFPVTFAKFDSKINKFIEMDCFESARQQRKRQTERRKKKLMEGK"
     gene            complement(2129..2371)
                     /locus_tag="IV228_001791"
     CDS             complement(2129..2371)
                     /locus_tag="IV228_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717683.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            complement(2368..3216)
                     /locus_tag="IV228_001792"
     CDS             complement(2368..3216)
                     /locus_tag="IV228_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8717684.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVSGSTIHAVITGRTGAGKTHLGAAVIKEVMMASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKTDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            complement(3216..4001)
                     /locus_tag="IV228_001793"
     CDS             complement(3216..4001)
                     /locus_tag="IV228_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717685.1"
                     /translation="MADKGWIALHRNIRDHWVYQEKRVFSKYEAWLDLLMDANHQNNK
                     FLFDGQLIEVNRGEFITSVRQLCERWGWSNTKVNRFLKMLEDDQMLIRKSDSKKTVIT
                     IVNYDFYQRHESKETTQKRQQNDAEASQKHTNNNDKTMNNNDNNNNPRNSRKTREYAD
                     DDPNKKLAILLLKLIRKNQNIKEPDLNKWANTIRLTIESDKRTGREVQDMIVWATSND
                     FWSGVILSPTSLRKHFDKMAVQKNKRKQQNISNDELPETGEDW"
     gene            complement(4018..4833)
                     /locus_tag="IV228_001794"
     CDS             complement(4018..4833)
                     /locus_tag="IV228_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717686.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVDDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            complement(4796..5818)
                     /locus_tag="IV228_001795"
     CDS             complement(4796..5818)
                     /locus_tag="IV228_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecT protein"
                     /protein_id="HAP8717687.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIRYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDPGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            complement(5821..6015)
                     /locus_tag="IV228_001796"
     CDS             complement(5821..6015)
                     /locus_tag="IV228_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717688.1"
                     /translation="MKDFDSLGARQQPQEEARAVGVDWQGNSLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            complement(6025..6249)
                     /locus_tag="IV228_001797"
     CDS             complement(6025..6249)
                     /locus_tag="IV228_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717689.1"
                     /translation="MISVKGLGDEIFEAMMHKAQQDVQDKILTAAKYGQTSCTVRSKG
                     LTPSFLAALESEGISNIQCEDGSVKLFWDL"
     gene            complement(6249..6578)
                     /locus_tag="IV228_001798"
     CDS             complement(6249..6578)
                     /locus_tag="IV228_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717690.1"
                     /translation="MNEKIQNLIKELAAECANDDLGMSVSVVDEKGEVVLGQAGNDSL
                     VAWSVYRQYEKTKRDLQNNNCNCETHGTLKQLFGIEYEDPEFEEWYQDFLRFAEKMDH
                     KKGGIKF"
     gene            complement(6610..7278)
                     /locus_tag="IV228_001799"
     CDS             complement(6610..7278)
                     /locus_tag="IV228_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717691.1"
                     /translation="MSYNKWTREEEQLLIQNVRYDHRGFVCNCNELAELLGIDKKRIW
                     SKIHQMRKKDLIDEVYWDDPIEPPKKRYSQQEDKKIISMYQSGCPVTIIAQELNRTEN
                     AIRCRLNIMKKNGQLESNRKRRYTKREVELLISEIKFDKNGYVLNIDYLTRLLHRPKH
                     ELFRKICLLRKQGEITIKPDRTKASQNWYDVMKKQIDNHHKLIAARYSYQKKTRTPAS
                     RIVS"
     gene            complement(7297..7569)
                     /locus_tag="IV228_001800"
     CDS             complement(7297..7569)
                     /locus_tag="IV228_001800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717692.1"
                     /translation="MTEITITDGHIFHTVKTKEEAQRIIDRYFPEGESTMQIRKPIVE
                     RSSSKMLLASIAEHKKPVYKVRQLQALCYISLVLNVILLSVVFAVL"
     gene            complement(7583..7849)
                     /locus_tag="IV228_001801"
     CDS             complement(7583..7849)
                     /locus_tag="IV228_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717693.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLVAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(7870..8625)
                     /locus_tag="IV228_001802"
     CDS             complement(7870..8625)
                     /locus_tag="IV228_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAP8717694.1"
                     /translation="MKELIKVTTNEENEQLVNGRELHEFLEVATEYKKWFSRMAEYGF
                     VENIDFVRVTQKCPTPGGIQNITDHAMKLDMAKEISMIQRTEKGKQARQYFIEVEKEY
                     KQQLLDTSKLSPELQMFQGIFNAVAKQELETKRLATQMENITEIVALNTTDWRRECRK
                     LVNKMAETQGGYGAYQEIQTAIYEEVDRRAGSSLKTRLTNLRNRMAGEGVPKSKRDKT
                     NKLDVIESDKRLKEIYLSVVKDFAIKYGVWKEK"
     gene            complement(8668..8916)
                     /locus_tag="IV228_001803"
     CDS             complement(8668..8916)
                     /locus_tag="IV228_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8717695.1"
                     /translation="MELQITLEAARVMMGYSLKEAAKLFDVHHQTLANWEQDPNKMKQ
                     KYVQLIPEIYHFPTANIFFGSKDEFIRYKLHNDSFLIK"
     gene            9081..9920
                     /locus_tag="IV228_001804"
     CDS             9081..9920
                     /locus_tag="IV228_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8717696.1"
                     /translation="MPRKPLSEEDKKARSIIANNLKKITHGYTRAQLSDMTGIPVTTL
                     SGYFTEKSTISMENAELIAKAFNVDKSDIDPRYSREYNYYDLKKEVLDELQENNKSFV
                     ADKFVELAEKANAAGMTLNRTKTDRGNIMTVLSSDIQEYNFFDTSVAAGIPSSVEAFD
                     QDHIEQIAIPDYAMGKYAGYQDVFFTKINGDSMNNVIPDGSLIAVKKIESCDELKDGD
                     IVVFSNDNEFSVKRYINDQQNNRIIFRPDSSNISFTDIVVNYDDTKYVQIYGKVVVYI
                     VQL"
     gene            9941..10141
                     /locus_tag="IV228_001805"
     CDS             9941..10141
                     /locus_tag="IV228_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717697.1"
                     /translation="MKIGMRKPSIKKSIGARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            10227..10667
                     /locus_tag="IV228_001806"
     CDS             10227..10667
                     /locus_tag="IV228_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717698.1"
                     /translation="MKFIKFGIIGLLVVTVMGGCGTSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLYSNLDPNKGVDTPLESDQ"
     gene            10805..12010
                     /locus_tag="IV228_001807"
     CDS             10805..12010
                     /locus_tag="IV228_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8717699.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLETDIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSVPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(12062..12149)
                     /locus_tag="IV228_001808"
     tRNA            complement(12062..12149)
                     /locus_tag="IV228_001808"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12115..12117),aa:Ser,seq:cga)
     gene            complement(12294..12860)
                     /locus_tag="IV228_001809"
     CDS             complement(12294..12860)
                     /locus_tag="IV228_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8717700.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            13115..14446
                     /locus_tag="IV228_001810"
     CDS             13115..14446
                     /locus_tag="IV228_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8717701.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            14412..14762
                     /locus_tag="IV228_001811"
     CDS             14412..14762
                     /locus_tag="IV228_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717702.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(14854..15273)
                     /locus_tag="IV228_001812"
     CDS             complement(14854..15273)
                     /locus_tag="IV228_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8717703.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(15274..15618)
                     /locus_tag="IV228_001813"
     CDS             complement(15274..15618)
                     /locus_tag="IV228_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8717704.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            15968..16564
                     /locus_tag="IV228_001814"
     CDS             15968..16564
                     /locus_tag="IV228_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8717705.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         4730 a         3172 c         2736 g         5964 t
ORIGIN      
        1 tattttactt gtaaattaaa tcgaggtgat aataatggaa attgaaaatt ttgaacaaaa
       61 aaaacagatt ttatcaaatt tattaattga tggttttgat aacgcaaact attcacataa
      121 acttttattt aaaagcgagc tagatgatga aaaagaattt gataagcaaa aagacttaat
      181 gtgcgcctta acgtatttaa atcaggctca cgccagcttc acaaatgcct acacctttat
      241 tgctcttaac gatgaacttc ttggtggtcg tcaagaattt gacaacatat tacatcagtt
      301 cactgaattt aatacagaat tcttaaataa tgtacgaacc aaccacagcc atcaatggag
      361 tgatatagaa tttaaacgtt tagtagattt ctttgaagca gcaagcggtt tattaaatgg
      421 ccacgaaaga attcaaggtc tgattaatga ggctagaaaa tagacaataa ttgtttccca
      481 gccgacctta cacgtcggct tttttgcttt ttttaacatt tctaactatt tagccgagca
      541 actattattc gttacgcgat attctttttc tatttatccc acttggtaaa aatcattggt
      601 tccggcataa tctgatcatc ctttagcaac gcatcaggat atttttttct tagcttctta
      661 ttattcgtat atgttttaag caaccattga cttgcttcac ttttgctttc accggaaaaa
      721 taaattttcc ctgtttggat gcctgcaagt tgaatcatca tttccacctc aaattagatt
      781 tttttctgct aaatggagca aaagtaatcc gacatcaaga atcttttgat ccattatttc
      841 agctactttg ctcggcttaa ctccttgaga aaagagcaag cgtgcttgct ctatctcttc
      901 gtgagtccaa ataaatttgg cttcctcaag gataattact ggatcagttg atctcattgt
      961 tcctctggaa aaatgatgtc gtccttatgt ttggaccagc tatctgcata tggagcaaag
     1021 taatgtctag ctaattcgat ttgatctatt aaagcaatct ctgataattc atgatctgca
     1081 gcaatttcac tcatactatc gccttctgct aaacggataa gaacgtctcg aatatttaca
     1141 gttacgtttt ctggtagttg caatgtcgtt gctgttttaa taaattcatc aatcgtttcc
     1201 tttgatacta ggattttcac attttcaact tcttctactc catcacctac atcaagtgaa
     1261 gtctgctctt ctttaagcat gtcgattgtg ccatcagcat ttacaacgta ttcgacattc
     1321 ggttttttcg tttgtttatt tactggcact gtgtattcga tagtttctgg ctggattact
     1381 acagtcactg ttgagccaag aaattcagtt aaattttcag tactacctct caatgatccg
     1441 ttgctaacaa ctagtaatac ttctgtgttc ccatttgatt ttgatgttac tttctttaat
     1501 tctggtctga aattcacctg ttttgtcatt ttattttccc tccattaatt tcttttttct
     1561 tcgttccgtt tgtcttttcc gctgctgtct tgctgattca aaacaatcca tttcaatgaa
     1621 tttattgatt tttgaatcga acttagcaaa tgtcactgga aagccgtatc gatgagcaaa
     1681 cattttcatt ttcagcatag agatcgcatc ttgataacct ttgacatcaa tcactttttt
     1741 cacttggcca ttctcataaa tcacaaaatc agctcggtaa taaatcggtg caatcttacg
     1801 atcgttgcat agataccctt cttgcagcag aacattttct tgcatggcac agtaggtttc
     1861 agataaaggc ataaaactca taccgtttcg ttttaacttc atgtaataac gagcttctgc
     1921 tttagaatca aaagtgatgc cgtctacttc gtgctttttg tttccgtatt tgcttcttgt
     1981 tgccatttgc tatactcctt ctttagtctt tcgagtcgtt ctttcgaacg tcgtccattt
     2041 tcattgcata ttggacaagg ttcacaagtt gaccatccaa gcgatgtctt ataccaaata
     2101 actgtttcat cgttgcattt taaacaagtc attttgcacc tctcatccat gcttggttac
     2161 ttttcggaca acaacactct tttttcactg gctttgtctt aggttttgaa aatcgtcttt
     2221 tgggtttaat aacttcgact acttcgccgc cacaaacact tgcaacaagg tcagcctctt
     2281 ctttagtcgt gtggtgttta gcaaactcga cactggcttc tcctcgccct cggctatcta
     2341 agtagcacat ttttttcact acaaaggtca aactggattc ctccttttgt ctgtcgttgt
     2401 tttgaatacg gctatgtttc cttttgtccc attcattaca cgagagaaca ctcgctcgcc
     2461 gtatgcgtgt tttaattgct tcgatgataa attggttgtg aatatcgtag ctttgtttag
     2521 acgagcttct gtgagcgatg tgagaacatc aacatcataa ggcgttgcct gattgttttc
     2581 ttccattctt cctagctccg cacctaaatc atcaatcact acaaaatccg tctttttaat
     2641 ttcagccatc aacgaacctg tcacagcttt tcttgcttct ggatcattca ttgcaaattt
     2701 caattgctct aataattctc gatagcttat aaatgaacag gcaattttat aattagatgc
     2761 catcattact tctttgatca ctgcagcacc tagatgggtt tttccggctc ctgttctacc
     2821 agtaataacc gcgtgaatcg tgctgccact tacgattttt ttagcccatt cttgagccat
     2881 cgcttttatt tgtgctgttt cctgatcaac tactttgtat ccatcaaaat cgaaggtcca
     2941 tatggcatca tctgtaatca ttgaactgtt aagcattcga ttgatagcgt cctttttcat
     3001 tgcttcattc gctttttgga tagcttcttt ttgattttta gttaccattt ctcgataacc
     3061 acaaatctta ttgatacatg taggaccgca tcgatcagta ccatctttat tttttgtttt
     3121 ccagatatac aatggatcac cacatttcgg gcaattatga tctgctgttt ttaatccgcc
     3181 gtaaggtgca gccattgcac ttagtttttt attcattacc aatcctcacc tgtttctggt
     3241 aactcatcat tagaaatatt ttgttgcttt cttttatttt tttggacagc catcttatca
     3301 aaatgttttc tcaggctcgt tggagataaa atcacacctg accagaaatc attacttgtg
     3361 gcccaaacaa tcatgtcttg aacttctctg ccagttcgct tgtcagattc aattgttaaa
     3421 cgaatcgtat tcgcccattt attcaaatct ggttctttga tgttttgatt ttttcgaatg
     3481 agttttaata aaagaatggc caatttttta tttggatcat cgtctgcata ctctcgcgtt
     3541 tttcgagagt tgcgaggatt attattgtta tcattgttat tcattgtttt atcattgttg
     3601 tttgtgtgtt tctgtgatgc ttctgcgtcg ttttgctgtc gtttctgtgt cgtttcttta
     3661 gattcgtgac gttgataaaa gtcataattg acaatggtta taacagtttt tttactgtcg
     3721 ctttttcgaa tcaacatttg atcatcttcc aacattttta aaaaccgatt tactttggtg
     3781 tttgaccaac cccatctttc acatagctgt cttactgatg taataaattc cccacgattt
     3841 acctcgatta gttggccgtc aaataaaaat ttattattct ggtgattggc atccatcagc
     3901 aaatctagcc atgcttcgta tttagagaaa actctttttt cttgatatac ccagtgatct
     3961 cggatatttc ggtgaagtgc tatccagcct ttgtctgcca tctactcacc caccgttcta
     4021 taaatcgtcc atactagtga atccagtaat tggaagatta cctctacagt actcgcacaa
     4081 accgcaatgg ttcggcttct cttcaccgtt cttcactctg acaattcttt caatattttc
     4141 ttttagcatg attagctcgt agctcatctt atcttgatca atcgttatca acttcgcctc
     4201 actaggcgtt tgcttcgata ccgcagcgat gattggcatg aacatctttc cgtattgttg
     4261 taaaagaagc tcttgataga ttgccatttg aagcacgtaa ccaaaattga caatgaacgt
     4321 ttctcttgat ccatagtttt cgttccattt ccgttcgtgc atgtcttttg ttgttttgat
     4381 gtcaacaaaa tactcatcgt caacattcaa gcaatcaatc ttgcctttcc atgtcgttcc
     4441 aaatagttcc ccagtcacga tcgtttcttt ctcaccttga taaatgttca tgaatgcttc
     4501 ttcttgctta agtcgttcaa tcatttgttc agcaatttgg aaatctttca acaatccata
     4561 cggcttccta ctagaaaaca ttttggattt attttcttct ttgaacgctt catgtacttc
     4621 ttttgattca aaataagaat gtacgtagtt tcctacgagt aaggctttgg gatccgacgt
     4681 cggtgcccaa tcgcctttca actttgctaa ggctgcggct tcacatttta aaaaggactt
     4741 atattgtgat gttgacatgt actgccagtc agcttcattg gagtaataat tctcatcaga
     4801 aagggtactc gtcgtcttca aattctgaga catcttctac accttcctcg cgtccaaagt
     4861 ctggaatatc tatcaattct tcttgaattg gattgatatc ctcattttcg atcaaatgtg
     4921 cttgctctat ttctggatgg gcaggtacat cagcaactcg agttacttca ttttggatat
     4981 catctggaat cacaatcgga tctggttctt tttgtattgg ttcagtaatc tttttgtttg
     5041 aaaagaattt ttgttcgaga gtagccactt tttctgtttg tggcgttaca tctttccgat
     5101 cattgtcaaa ttcattttct aacgtatctt ttgcagcttg aactaataaa tcgttgtcgt
     5161 tgcttgtgtt gatcaatgtt ttagctgccc gattgattac tgtccgttta gccatttctt
     5221 ctggaaattc cttttgcggt ccaggatctt tcatttttga cttagcccaa ctttgatcga
     5281 tttgcttttt cgtcatgact gttgtggtct ctttaccatt tgttaacttg atgactacat
     5341 aggctgcaat gatttcattg tctaaattct cgaattttgt ttcatgcttt ttgacaacca
     5401 tgtttggtcc atccattgca acttcaaact catcaccttg tcgaacgact acaggcgtaa
     5461 tatctgcccc tccagttaca cgatcaagga ccgccattgt tccaaaatat gaacgcatta
     5521 gctgaacctt gtttccgtat ctaatgaaat agcattgttt ttttgctgga gataaccctt
     5581 gaataaccat gtctaaaagt gcattagaaa tggaggtttt catttcttga ttgtttgcag
     5641 ccatctgcaa caagtttcct cctgtattgt tagtgagttc gaaaaaagca ctttttaacg
     5701 cattttgtgg actatatcct ggtggcattt ctaaaccttg ttcttgtaac cgtccaagat
     5761 tactgattac ctgctcatct aatgaacgtt gtgttgtttg tgttaaatca tttgccattc
     5821 ctaaattcct cctaattcaa tctttggata atgttgctgg acgtactcta gaattgcgtc
     5881 tactggtaca tagccttctt ctgttagata gcaagtatca cctggataca aagaatttcc
     5941 ttgccaatca acaccgacag ctctggcttc ttcttgcggt tgctgtcttg cgcctaaaga
     6001 atcaaagtct ttcatcacgg cctcctaaag atcccaaaat aatttgacac taccgtcttc
     6061 acattgaatg tttgaaatac cttcactttc taatgctgct agaaatgatg gtgtcagccc
     6121 ttttgaacga acagtgcaac ttgtttgtcc atactttgct gcagttaaga ttttgtcttg
     6181 tacatcttgt tgtgctttgt gcatcattgc ttcgaatatt tcatcgccta gccctttaac
     6241 tgagatcatc agaatttgat accacctttc ttgtgatcca ttttttcggc aaaccgtaga
     6301 aagtcctgat accattcctc aaactctgga tcttcatatt caataccaaa caattgtttt
     6361 aaagtaccat gagtttcaca attgcaatta ttattctgca aatcacgttt tgttttctca
     6421 tactgtcggt aaacactcca cgcaaccaag ctatcgtttc cagcttgtcc aagaacgact
     6481 tccccttttt catcgactac actaactgac attcctagat catcattcgc acattcagct
     6541 gctaactctt taattaaatt ttggattttt tcattcattg tgataaactc tccttgtaat
     6601 tagtattttt caggacacga ttctgcttgc cggcgttcgt gtcttttttt gatagctata
     6661 tcgagcagct atcagtttgt gatgattatc aatttgtttt ttcattacgt cataccagtt
     6721 ctgactagcc tttgtacgat cgggttttat agttatttct ccttgtttcc ttaatagaca
     6781 aatttttctg aaaagctcgt gctttggtct atgcagtagt ctagttaaat aatcaatatt
     6841 taatacgtaa ccatttttat cgaatttaat ttctgatatt aataactcaa cttctctttt
     6901 tgtgtacctg cgttttcgat tgctttctaa ttgtccattt ttcttcatga tattaagacg
     6961 acagcgaatc gcgttctcag ttctattcaa ttcctgcgct attattgtta ctggacaacc
     7021 tgactgatac atagaaatta tttttttatc ttcttgctga ctgtaacgtt ttttaggcgg
     7081 ttcgattgga tcatcccaat aaacttcatc gatcaaatct tttttacgca tttgatggat
     7141 tttggaccaa atacgctttt tatctatccc aagtaactca gctaattcat tacaattaca
     7201 aacaaatccg cgatggtcgt atctgacatt ttgaataaga agttgctcct cttctcttgt
     7261 ccatttgttg tacgacaatt ttctaacctc ctttttttat aaaacggcaa agacaacaga
     7321 caataaaatg acgtttagaa ctaagctgat atagcaaagt gcttgtaact gacgaacttt
     7381 gtatactggc tttttgtgtt cggcaattga tgccagtagc attttgctac tcgaacgctc
     7441 aacgattggt tttcttattt gcatagttga ctccccttcc ggaaaatatc gatcaataat
     7501 acgttgtgcc tcttcctttg ttttgacggt gtgaaatata tgtccgtctg tgatggtgat
     7561 ttctgtcatg tacatcctcc ttttagattt tgtatttagc catcagctca tcgatgtctt
     7621 ttttgtcata ccttaaaaag ccatctatac gaatttcttt taacccgtga gctactaact
     7681 tctcaaagcc ttgatcattt acacctccca catattttct agcttcgatt tttttcaagt
     7741 atcgtgtttc tgacaaattc ttttttgaaa gttgactaag tgcgtccttt acaacttcaa
     7801 caattgcaat tctcaaacga gtcaaaaact cttcgcttag aatattcatg acttgcctcc
     7861 tatcgaattt tatttttctt tccaaactcc atacttaata gcgaaatcct tgactacaga
     7921 tagataaatt tcttttaacc ttttatcgct ttcgattaca tctaacttat ttgtcttatc
     7981 tcgcttggat tttggaacac cttcgccagc catacggtta cgtaaattgg ttaatctagt
     8041 tttgagagag gagccagctc tacgatcaac ttcttcatat atcgctgttt gaatttcttg
     8101 ataagcaccg tatccgcctt gggtttccgc cattttattt acgagctttc gacattctct
     8161 acgccagtca gttgtattca atgccacaat ttcagtaata ttctccattt gtgttgctag
     8221 acgttttgtc tctaactctt gcttagctac tgcgttaaat attccttgga acatttgaag
     8281 ttctggactt agctttgaag tatcaagtaa ttgttgtttg tattcttttt ctacttcaat
     8341 aaaatattga cgcgcttgtt ttcctttttc tgttcgttga atcattgaga tttcttttgc
     8401 catgtccaat ttcattgcat ggtcggtaat attctgaatt cctccagggg tcggacattt
     8461 ttgggtcacc cttacgaaat ctatattttc aacaaaacca tattccgcca tacgactaaa
     8521 ccattttttg tattctgttg ccacctccaa aaactcatgc aattctctgc cgtttactaa
     8581 ctgctcattc tcctcatttg ttgttacttt gattagttct ttcatggatt acactcctta
     8641 aaaatgaata aatatgtttt tcataagtta ttttattaaa aatgaatcgt tatgaagttt
     8701 atatcgtata aactcgtctt tgcttccaaa aaaaatattt gctgttggaa aatggtatat
     8761 ttctggtatt aattgcacat atttttgctt cattttattc ggatcttgtt cccaatttgc
     8821 taaggtttga tggtgaacat caaagagttt tgctgcttct tttaaagaat atcccatcat
     8881 aactctagct gcttccaaag taatttgtaa ttccattttt attcctcctt cgtttatgta
     8941 aataatttta tactagttta tactaccagt caacgatttt tgacgttttt taaattaaaa
     9001 aacactgtta taaaactact ttatacttgt ttatattgat ttaattcgat attattaata
     9061 aaaaaggagg agaaaataac atgccaagaa aaccattatc agaagaagat aaaaaagctc
     9121 gctccattat cgctaacaat ttaaaaaaaa ttacgcatgg ctacacaaga gcacaattgt
     9181 cagatatgac aggtattcct gttacgacgc tatcgggata ttttactgaa aaatcaacta
     9241 tcagtatgga aaacgctgaa ttaatagcta aagcttttaa tgttgataaa tctgatatag
     9301 atccaagata ttccagagaa tacaattatt atgatttaaa gaaagaagtt ttagatgaat
     9361 tacaggaaaa caataaaagt ttcgttgctg ataaatttgt agagttagca gaaaaagcaa
     9421 atgcagcagg gatgactttg aacagaacta aaactgatag aggaaacatt atgacagttc
     9481 tatcaagtga tattcaagaa tataactttt ttgacacttc tgttgctgcc ggcatacctt
     9541 cttcagtcga agcttttgat caagaccata ttgagcaaat tgctattcct gattatgcca
     9601 tgggaaaata tgcaggatat caagatgtat tttttactaa aataaatgga gattccatga
     9661 ataacgttat tcctgacgga tcattaattg ctgtaaagaa aatagagagc tgtgatgagt
     9721 taaaagatgg agatatagta gtctttagca acgataacga attttctgta aaaagatata
     9781 taaatgatca acaaaataat cgcatcattt ttagacctga ttcttctaac atttctttca
     9841 ctgatattgt ggttaattat gatgatacta aatacgttca aatttatggg aaagtcgttg
     9901 tatatattgt ccaactataa ttaatttgag agggtataaa atgaaaattg gaatgagaaa
     9961 accgagcata aagaaatcta tcggcgctag aactactgga aaggcaaaaa gagctgtgaa
    10021 aaaagctgtg attcctggtt atggaaagaa aggctctgga tggattaaag acccaaaaaa
    10081 agctgcatac aataaagttt ataaaaagac tacgtttagc ttttgggact tatttaaata
    10141 aaaaaagccc gtgcagcaac acggactcga aaccttattt ctaagattcc ccaataaaat
    10201 aatatcatag agataaggag tagaaaatga aatttattaa atttggaatt attggattac
    10261 tagtagtgac agttatggga ggatgtggaa catcaacagc tgaaacaaag gaatcaaaaa
    10321 attatagtga gacaaacagt actatcaaag aagtagactc tgatcaaacc gctgagtttg
    10381 caactattgc cgagaaacac attaaagaaa tatattcaat agataatttg aagatggatt
    10441 taaaatctat caaagttact caatttcctg acgataaaaa tgcagaaact ggtgaagagt
    10501 acaaaaatgt ctacaatggt actggagatt ttacttggca agacaaaaaa tacaactttt
    10561 ctttgattta ttcgaaaaaa gatgcaacta attataccgt cctatatcta tatagcaatc
    10621 tagatcctaa taaaggtgta gatacaccat tagaaagcga tcaataaaaa aagcacattt
    10681 gccgcccgac caagaacgca aatgtgcaaa gagctacggt aaagtaggtc tatttgttgt
    10741 accctatttt accatctcga aatccgctgt acaaccgaac aaacgtacga aaggaaagat
    10801 aaagatggca aaaaaagaaa ttgataaacg aattaaaaag cacttaacaa aaaaaggcga
    10861 agaaaaatat cagtttagtc tgtacttagg agttgatccg ctaacaggaa aaaaaagaaa
    10921 aacaacgaga cgaggtttta gtacgccatt agccgctgag agagctttaa aaagattgga
    10981 gacagatata caggaaaaag gactgcaagc ttctacagct caaaagagca agaaattcga
    11041 atatgtttac ggcttatggt ttgaaaatta taaaaagaca gttaaagaaa gcacttggtc
    11101 ctccaccaaa caaatttttg acacacacat cttaaaagtg tttggagata agttcattga
    11161 caaaatagac gtttttttct gtcaagaagc agtaaacact tggtccgata gccatcctaa
    11221 aatttttaag aaaataaaaa attacacatc taatgttttt gattacgctg cttctttaca
    11281 aattataact agcaatccta tgaaattagt ttctgttcca cgcggagaag ctctagacat
    11341 cgaagataaa aatatcgaat tttatactaa agaagaatta atcgaatttc tagaagcaat
    11401 aagaaacgat gatgatgaaa gatatttatt cttttcccta ttggctttta caggtataag
    11461 aaaaggagaa gcttttgctc tgacctggtc ggatattgac tttaagagca aaacactcaa
    11521 tatcaataaa actgtaacaa gaggttatca agggagattg atcgtaaata ctcctaaatc
    11581 aaaatctgga aaaagaaaaa tttatctgga caatgatttg atcagtttat tgagaaagta
    11641 ttatacaaaa aacaaaacga tcgttacaat ccaaagcgaa aacttaattt ttcaccatga
    11701 tggtcttctc tataacccta cagtttcacg ttcgtggctt aatgtcatat ataagcatca
    11761 tccagaattg actaaaagaa tcactactca tggttttcga catactcatg cctctctcct
    11821 ttttgaatca ggagcttctt taaaagatgt tcaggaacgt ttagggcatg cagacattca
    11881 aactacttca aatatttaca ctcatgtaac tgaaactcaa aacaaaaaag tcatcaataa
    11941 ttttgtagca tttatgaaga atagtactca gggggagtca aaaggggagt caaaaaatat
    12001 tttgggctaa acatacaaca aaaaagacac gatcgaaatc gtgtctaatc gttgatatat
    12061 aaggaaggta caggatttga acctgcgcgc cggtattagc cggttcgccg gatttcgagt
    12121 ccggtgcatt accactctgc caaccttccg taagtactat ttttaaactt ttttcacgaa
    12181 atgtcaagcc tatttctaat agataaataa cagaaatcgc tttttttagc aaaaaagtcc
    12241 attgacagtt tgcagccaac ggacttaccg atcaaattca tactgataga acactatctg
    12301 attatttcag gattatctaa tgctaaaatc gagtcaattg tccgtttgat cagtgcaatg
    12361 atttgttctt ctgttggatg catgatgttt gatgacatca atgacgctaa gccagtcact
    12421 gctacccatg taccagtatg cagagcatcg acatattcat ccgataattg attgtatctt
    12481 tcatcttctt taactatttc acggaaataa ttgtatgaaa aatcatgcat tttctttccg
    12541 tccccatgtt catcaatgta caatgccatg taaagactat gatgcttttt tgcaaagtag
    12601 atatagttca aaccgagatc aatgatttta tcaccagtac gttcaatagg gaaaacatct
    12661 tcttttagtt gttcaaagat agaatccagc aacgtatttt ttaaatcttc catgtttttg
    12721 aattccagat aaatcggctg tgtcgaaatc cccatctttt tagcaatatt tctcgcagta
    12781 aatcctttaa acccttcctt tgcaacaaca tcataagcag cttttagaat ttgatccttg
    12841 gtatagactt ttcttctcat taaaactccc ccacttcttt actgcagtat atgttgtttt
    12901 attgtaccct atttttagca ttttttgcaa acattttact caaaacgtta tattttaaat
    12961 ttttttcagt cttctttaac ttttttttgc aaaattaaaa aaatttggtt aaattcaatg
    13021 ggttccctta catttttagt tttgacctaa atgatagttg tccaaatctt tcatttcaag
    13081 ttatagttaa agaaaatgaa aaagaaggtg tgtaatggaa aacgaaactg acgttattta
    13141 tattcatcct caaaaacgaa tcgtttcaca gaaaagaaag tatttttatc ttggttttac
    13201 aggtgttttt tttcttttta ttggcttgct ttctaatact cctactgata attggagtgg
    13261 attgctaact attcttacct cgccaagcaa tcttttgaca gattattttg ctttaggtgt
    13321 tttcggaagc gcattcataa atgtagggat tttgacctta ctttctgtgc ttttagctta
    13381 tcggcacaag gtgattttaa acggtccctt atttgcttcg attctaacag ttaccggttt
    13441 ctcttttttt ggcaaaaacc tttataattc tatctcaatc attttaggtg tgtatctata
    13501 cgctgtattt gtcaataaac ctttttctca gtacatcatg atcggactgt ttggtagcgt
    13561 attatcaccg gtcgttagtt atatcacttt tggtatgcgt tttcctttac tgataggtat
    13621 tttattgggg aatttagctg gcatagcaat tggtatactt cttccgccct tagctgctca
    13681 aactttagtt ttccatcgtg gattcacact ttataatatt ggattcacct ctgggttgat
    13741 tgcgatgact tttactgctg ttttgaggtt attcagctat tcaatcgtcg aaaatacatt
    13801 ggttttcaat gaatatcact ttcctctcat ttggattatt tttggctttt tttcgctgac
    13861 agtcggaatt ggtttctatt ataattcctt tcggctttcg gggattcgag aaattttcga
    13921 ttcatctgga aaattgacaa ctgatttcat tgctaattct ggtattggtg ctactttgat
    13981 caacatgggc ttagttggtc tgatgctgtc ttcttatgtt ttgctggttg gcggtcagtt
    14041 aaatggacct gtcatcggtg ctattttatc tgcggtgggt ttcagtgctt ttggttgtca
    14101 tttaaaaaac agttttccga tactagtcgg aatatttatt gcttctttgt ttggaacttt
    14161 tcatgagatc accagtactg gcatgcttgt tgcagctgtg tttgggacag gtcttgcgcc
    14221 cataagcggt ttttacggca gtttttacgg tgtaatagct ggtgtgctac atattgctct
    14281 tgtccataac gtcagcactt tgcacggagg actaaatcta tataacagtg gtttttcaac
    14341 tggtttcgtt gcaggtatcc ttgtaccgat actagataac tttaccgctg tcagaaagga
    14401 aaaaaagaca cttggaaaaa gaattatcaa aaagaatcat cgatgagctt cttacttatt
    14461 tatattccca tgaaataact gaaatacgta ttggagtaga atttgctgag gaagggcttt
    14521 tcatagagat acagggtacg gcagaaactc agccagaaga tttagatgta cttgctgatt
    14581 tgctgaacaa tcctcgggac cctagtatgg aaatctacta tcaagaacta ttaggtaatc
    14641 cccgacatga tcgggaagat tatcatctgt taggtagttt gattgatgac gtggaaatca
    14701 tgtatgccca gccgattatc aatattaaga tctttcgacg tgcttctcat tcctattatt
    14761 aaccctattt tctatttata aaagactgaa tcaaaaaagc agctcctgaa agttcgatta
    14821 aaatctactt tcaggagctg ttcaagcttg acattattga ttttccaatc cttcatacac
    14881 ttcatctgtt acaggttcta aaaactctga attaccagca gtgatcgcca aatgttcaaa
    14941 ccaactgtct tttgtcgcac catgccaatg tttaatccct ttgtgcgtta cgacgacatc
    15001 tccagccatt aatcgtcttg ctggctttcc ttcttcctga taccagcctt ctcctccggt
    15061 cacaagtaag atttgatagc cgtctgtatg gacatgccag tgattgcgac aacctggttc
    15121 aaaagttaca ttacctacac caacagaaac ttcgggatca ttcactaaca tattcagata
    15181 gctttgcccg gtaaatactt gagcgtaagc ttcgtttttt tcacctgctg gaaaaatcac
    15241 gccattgtgt atttcttcgt gttttgtcat catttacgcc tctttcttat cttcgctatt
    15301 ttcttctaaa tagatttctt ttgctaaact aaaagctgac caagcttttg gccattctac
    15361 ataaaaagct aaatgggtaa tgatttctgc tatttcttca ctagtaatgc cattttgctt
    15421 gccaatcttc agatgagctg gcaattgttc aaaatttcct cctgaaatta atgcagatat
    15481 ggtgatgata ctacgctgat gagcagataa ttcttctctg gaccatactt gtccaaataa
    15541 aacatcatca tttaattctg caaattttgg tgcaaaatct cctaattgat ctcgtcctgc
    15601 ggtttgtttt tctgccattt ctcttattct cccttcacga ctgttaggtg attcaactat
    15661 ggttctaagg caactttatt ttctattgtt tcttcgcatt tcattttaaa tgtagtcttt
    15721 cagcctgtcc aatgcttagt ttattcagtc gatatgcttt ttaagcatag ttgctaacgc
    15781 tttttttatt ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag
    15841 atcaatcttt tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa
    15901 atggcgcagt tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg
    15961 atgaataatg aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt
    16021 aggcctatca atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa
    16081 tgcgttaaga ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat
    16141 ccaaatcctg caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt
    16201 cctgatattt ggcccttatg ctggatttat ctataactat attggaatct gcattggttc
    16261 gattatcatt tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag
    16321 cgataagaca tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct
    16381 gtttgctcta gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg
    16441 attgacaaac atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc
    16501 gattttctta tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg
    16561 ataatattta ttttgtaaaa ttagtcattg attgacaatg tt
//
LOCUS       DACDHL010000052        16558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDHL010000052 DACDHL010000000
VERSION     DACDHL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.052"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..468
                     /gene="scm"
                     /locus_tag="IV228_001815"
     CDS             <1..468
                     /gene="scm"
                     /locus_tag="IV228_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8717706.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(623..1609)
                     /locus_tag="IV228_001816"
     CDS             complement(623..1609)
                     /locus_tag="IV228_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8717707.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1632..2198)
                     /locus_tag="IV228_001817"
     CDS             complement(1632..2198)
                     /locus_tag="IV228_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8717708.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2212..2628)
                     /locus_tag="IV228_001818"
     CDS             complement(2212..2628)
                     /locus_tag="IV228_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8717709.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3085..5019)
                     /locus_tag="IV228_001819"
     CDS             complement(3085..5019)
                     /locus_tag="IV228_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8717710.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5321..6256
                     /locus_tag="IV228_001820"
     CDS             5321..6256
                     /locus_tag="IV228_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8717711.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6346..7449
                     /locus_tag="IV228_001821"
     CDS             6346..7449
                     /locus_tag="IV228_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8717712.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7462..8415
                     /gene="menA"
                     /locus_tag="IV228_001822"
     CDS             7462..8415
                     /gene="menA"
                     /locus_tag="IV228_001822"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8717713.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9350..10696)
                     /gene="gor"
                     /locus_tag="IV228_001823"
     CDS             complement(9350..10696)
                     /gene="gor"
                     /locus_tag="IV228_001823"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8717714.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10830..12071)
                     /locus_tag="IV228_001824"
     CDS             complement(10830..12071)
                     /locus_tag="IV228_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8717715.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(12089..13012)
                     /locus_tag="IV228_001825"
     CDS             complement(12089..13012)
                     /locus_tag="IV228_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8717716.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13233..15725)
                     /locus_tag="IV228_001826"
     CDS             complement(13233..15725)
                     /locus_tag="IV228_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8717717.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15732..16199)
                     /locus_tag="IV228_001827"
     CDS             complement(15732..16199)
                     /locus_tag="IV228_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8717718.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16335..16408)
                     /locus_tag="IV228_001828"
     tRNA            complement(16335..16408)
                     /locus_tag="IV228_001828"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16372..16374),aa:Arg,seq:acg)
     gene            complement(16466..16539)
                     /locus_tag="IV228_001829"
     tRNA            complement(16466..16539)
                     /locus_tag="IV228_001829"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16503..16505),aa:Pro,seq:tgg)
BASE COUNT         4887 a         3460 c         2944 g         5267 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct
      121 accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct
      181 accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc
      241 acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg
      301 aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt
      361 cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta
      421 atcggtttaa gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa
      481 gataaaaaag agcaacgtcg ctaacggacg ttgctcttta tttttgcttt ttatcataaa
      541 tacaagaact acaattcaat ttgtcaaaaa acacgtagat ttccttctga gaaatttatt
      601 tacgtatcga acctttccct attcagtctt tgcgatccaa aatcagttgg gtaagggaaa
      661 agagattctc ttttgttcga agagaggtgg caggtaattt ctttatttcc ttcaaagcct
      721 tttctgtgta agaggccgct aacttcctag cttcttccac gcctcctgta tgttcaacga
      781 ttttgcgtac ggcgtcgaca tcttcttctg tcattgccgc tttcttctct aaataaggta
      841 gtagctttgg tttgtttttt tgcaaagagt agatcaacgg taaagaatag accccttgac
      901 gcatgtcttc tagtacaggt ttgccgattt cctggctctc ttttgtataa tctaaaatat
      961 catcgataat ctgaaaggcc atcccgatag aaagtccgat atctcctgct ttttttgcga
     1021 agcgctcgct tgttccactt tcaaaagcgc caacagaaca acttaattgg aacagttcag
     1081 ctgtcttacc tgaaatattt tccaaatatt cttttacaga aagttcataa ttatagcgat
     1141 catccatctg ccctaattcg ccattcaata ctttttccat actgcgagaa ttcaattgga
     1201 gacttttcaa ggaagtggag taatcagaca atagtttaaa acagcagaca aataaatagt
     1261 ctcctgcata gacagaaacg acattaccaa actgactacg gatattaggc tgaccacgtc
     1321 gcagatccgc ttcatcaacg atatcatcat ggatcagtgt ggccgtatgg agcatttcga
     1381 tagaagctgc caaagcgatg gctttgtctc gatcttgctc tggtccaaat tgtgagaaca
     1441 atagctgata ggcaggacgc aacagctttc cgccggcatg gatcgtttct ttgattgctt
     1501 gttcgacagg tttgtttttc aggcggacag tttcttccat ccgttttagt gtttctgtca
     1561 attctttact taaagcagga tagttttccc acattggatg tagcttcata aaaaaactcc
     1621 ttgtgtgacg attatttttt ggtaaacatt gggctccatt tttgtttggt tccctgctca
     1681 taatcttttt ggaatcgttg aagtgtctgc acatgtcgta aaagataatt cgctgcgatc
     1741 ccgattgcaa tccctgataa aatccctaaa actgaaagga ctggcaagta caacatgact
     1801 gtccatgatt gtgcaatgaa agaagccatc aaaagctgtc cgacattatg catgaaaccg
     1861 ccggccgcac taattccgat gatactgaca cgttttggtc ctaattgctt gaccaataac
     1921 attccaaaat aactaagcaa agcaccactc atactataaa ggaatgtgga aatcgttccg
     1981 cctagcaaag tagtcaggaa tagtcgtagc caaagtagta agaagctgtc ttgttttttc
     2041 atggtaaaaa tggcaacgat cgtgattaga ttggctaagc ctagttttgc accaggcgca
     2101 aaagaaaaag gataagggat catattttcc aataaaccaa tgatcactcc ttgtgcaaca
     2161 agtagcgaaa tataaatcat tttttgtaat ttactcatgg tgtgtaaagg tctaataaat
     2221 cagactgtct tcgtcacctc catctgttga acggacttcg atcactaatt tatgtggcaa
     2281 acagacgatg gtttcgccat ttttcgttgc ccagcctcga cgtacgcaga tctggtcatc
     2341 gcaatctgct tcttttatcc gtactcgatc gccatctatc tcgatcaagt tataatctcc
     2401 gtgttcatct ttgtaagtat atgtgttgct ctcttctcca gccatcaaag ggaatgtttt
     2461 gatttctgtg ccatccacac gcagtaccgc ttctttttct gctgttgcgg tgttttgctg
     2521 aagaccaaaa atcatgactg gtaaaaaaga aagaacagtc aaaagcaaaa taataaaaac
     2581 atcccaaggt ttgagcagac ttttttcagc aaattcttta aggctcattt ttgttctcct
     2641 ttttccagat taactcaccc aattttagca taaaaacaag aggttgtgac acctgtgtct
     2701 tgctttgagt aataagaacg aagaatagaa aacagctttc catgttttct aattctttgg
     2761 gcttattacc gagaagcagc aggtggaaca ccgtttataa caagaggttg tgacagaagt
     2821 gggcaacttc aagaaataag aaggaattta cgaaaattac ttttcaagtt tttgtgaaat
     2881 ttcggcttat ttttcgaaga agctacttct ggaacaccgt ttataacaag aggttgtaac
     2941 agaggttgtg acacctgtgt cttgctcatg gaggcttatc tcgggcatct tattccttta
     3001 attttacata aaaaggaggc tgtgacggtt tgtctcagcc cctttctatt gaaatcttgc
     3061 attcaaataa cgttctttat ctatttattt cttgccgtac tgggaacttg gtctaccgta
     3121 ccaccaatta cctagtgtac gttgtagcca tggaattacc cagcgatcca acccaatcgc
     3181 acgtccggaa ccgttcatca atgcgaaagc aacaaaaatg aaccaaatgt ttacccagta
     3241 aaacatgcct gataagcaga acgtgatcac caatacaatc gttgtcgcac tgcatagcca
     3301 tgtgaataat ccagcaatca atgctaatgc caaggcaatt tcaaagaata ccatgaattt
     3361 ttggaagaac attgctacat cattgtttgg catgatcact tgcatgatct ttgtgaacca
     3421 gtctggtgac ttctcaatca cagccattgg ttcttcaccg tatgcataac tcaaaccaaa
     3481 atgtgcagct tgagtcgctg cttctccggc acttgcccct gctccgctgg cagctgtggt
     3541 tgcatcacca gcagcagaag caccactggc aacatctgtt acattgtatt cccatgtcca
     3601 agggaacaca ctgtttccag tgaaccatga tccttcacca aaccaagtac ttggtttgaa
     3661 cacttcacca ttcccaatga tttttttgga agcttcaacg aaccaaacta aaccatagaa
     3721 aatacgaagt ggcacactcc ataagacatt gccgtaacga gaagaatgtc ctcgagtcac
     3781 gttgcgatca tctttgatat ggaaaaactc atgcattaaa tattggaaca tataataacc
     3841 agaacgaata tcaaagaagt atttcaaatt cacgatatgt ttcattacga tagctaaaaa
     3901 tccgcttaaa tggatcttat caaataggtt agctaccccc catttggcac caatagaaac
     3961 cataaatcct tgatagtttc ctttaaattt atgcttttct ccgcctttga tcgaagccac
     4021 gatattcgct gcggctgtgt gtcctgtttg ttcagcagct tgaacgatct gcggtgtcgg
     4081 tgttcctggg aattcttcat aaaagactaa atcaccaatg atatagatgt ttttatcttc
     4141 gtagcctttt gcctgcatat attcattggc gatcaaccgc tgtccgcgtg ctgattctaa
     4201 tccaaagtca gcagcgtcag aagttccttt gacaccagct gtccaaatca gtgtgtgagt
     4261 cggaatgttg cgtccgtctt tcaatttgat atgatcagca gccacttcta cgattggtgc
     4321 attcaataat aattccacgt tgtgcttacg caaatagcgt tcagcttttg cagcatcatt
     4381 tcgatccaac atattcaaga tcgtcggcat tgcctctacg accatcagtg tgatttcgtt
     4441 tggatctaat ttataatctt tggctaggcg atttttccag tcgatcagtt ccccaatcat
     4501 ttcaataccg gtaaatccag aaccacagac aacgaaagtc agcatggctt tacgcagttc
     4561 atcatctggt tccaatgcag cttttgctac tgtcttctca atatgttccc gaatccttac
     4621 agaatcttca aatgaccaaa gggtgaatcc gtgtgctttg acaccaggtg taccaaagtc
     4681 attcggctct ccgcccattc caaggatcag ctgatcaaaa ggataggaac ctaatttggt
     4741 cttgacgatt tttcgttctt tatcgatacc gattacagtg tctgtgacaa gttgtacatt
     4801 cttttgacga gcaaacaaat gctgcagatc gtattggatt gcttctggtt ctacacgtcc
     4861 gccagctact tcatgcaact cggtcatcat agtatggtac gaatgacgat cgatcagagt
     4921 aattgctaca tcttcttttt tcagtttctt tgccaagtat tttgtagctg aaacaccggc
     4981 atatcctgct cctacaacga caatattttg ctttgccatt gatcatccgc tccttataga
     5041 ctatctatat aatgaatata tgataattct tttagtttga tacttattac acaatctcca
     5101 tatattatat agatgttctg ttcttttgtc taactccttt tggatttttt tcaaatttta
     5161 aaataaagcg ttttttcacg aaatttgctg aaagaaaacg tttgaaatat tacatgggtt
     5221 atttatcaaa aatagttgac ttataggcta aaaagattag aatgtataat gaatcacaaa
     5281 gaaaaattgt ggactagaaa tggaatggtg gggtattaaa atgaagaaaa aacgttttgt
     5341 aactggattt gccgtattag cattttcagc tctagtgtta ggcgcttgcg gcgcagatga
     5401 caacggaagc tcaaactctt cttctgaatc atctacagca caatcttcga ctgcaaaaag
     5461 cacaacagaa tcatctgcaa aagttgtagc tggcggagat ttgcaagacg gcacatataa
     5521 attagaagaa aaaaattatt caaatggtta ccgtgcagtc tttgaaatgg ttgtaaaaga
     5581 cggcaaaatc actgaatcaa aatatgataa cgtcaatgaa aacggcgaat caaaaacaaa
     5641 agatgcagaa tacaacaaaa acatggaagc aaaatctggc acaaacccag aaaaatacat
     5701 tcctgagttg aatgaccaat tcttaaaagc acaaagcgca agcggtgtgg aagtcgtaac
     5761 tggagctact cactcatctg aaagcttcca aaactacgca caacaattga tccaagctgc
     5821 tcaagctggt aacacagata caatcgaaat cgacaatggt gctgatttga aagacggcac
     5881 ttacaagctt gaagaaaaga actactctaa cggctaccgt gtgcaattcg aaatgacagt
     5941 tgctggcggc aaagtaactg aatcaaactt tgattatatc gacaaagacg gtaaatcaaa
     6001 acaagatgat acagaatata atgaaaatat gaaagcaaaa agcggtacag aaccaaaaac
     6061 ttacatccct actctgaacg acgaattcgt caaagcaatg ggcgaagaag atggctcacc
     6121 tgctaatgta gaagttgtta caggtgctac acacagctca cattcattca tcatgtacgc
     6181 acaacaatta gtgaatgcag ctgaaaaagg cgacactcaa acaatcgaag tagataatat
     6241 cgtaacagaa aaataatcac ataatcatac taataaaagt tggagggtgg gacgatagcc
     6301 agattaggca gtcgtctcac ctttcaaaat gaagagagga aaaagatgcg gaaaaaacta
     6361 ctattttcct taatcacact gggcatctct tttatggtgc taactggctg cagcaactca
     6421 gcaaataaaa caaccactga aagttcagca gctaccaaga tattgaaaga tccatattct
     6481 gatgaacagt tcttgttagg gacttacgtt cgtatccgtg tgtatgatga aggcaaagaa
     6541 tcagcattga aacctgcctt cgatcgagta aaagaacttg gtgataaaat cacgatcaat
     6601 caaaaaggtt ctgagatcga tgaagtgaat gagcaagctg ggatcaaacc agtaaaagtt
     6661 tctgatgacg tctacacgtt agtcaagcgg gcctacgaat atagccaaga ttctcaaggc
     6721 ggattcgata tggcgattgg tgcaatcaca caattatggc gaatcggttt tgatgacgcc
     6781 agaaaacctt cccaagaaga aatcgatcag gcattgaaat tagtggatta tcataagatc
     6841 gaactaaatg acaaagaaaa aactgtttat ctaaaagaaa aaggaatgat catcgattta
     6901 ggtgcaatcg ccaaaggtta cattacggac gaagtagtga aagtcttgaa aaaacaagga
     6961 gtgaccactg ccatcgttga cttgggcggt aatgtctatg tcttaggcca cagcccaaga
     7021 ggcgaaaacc aagactggac agtcggcatc caagatccga acatggctcg cggatctgtt
     7081 ttaggttcta ttaaagaacg gaacaaaaca ttagttacct ctggaattta cgagcgttat
     7141 ttagaagtag atggcaaaaa ataccatcat ttgtttgatc caaaaacggg ttatccgttc
     7201 gacaatgata tcgcaagtgt cacgatcata acagataaat caattgatgg tgacgggttg
     7261 tctacagcag tcttttcaat gggtgtgaaa aaaggactgg aatacgtcga aagcgaattg
     7321 aataacggga cggaagcgat cttcgtgaca aaagacgata aagtatatgt gaccgatcca
     7381 atcaaagaca ccttcaaatt atcggaggac tcccattaca cgatgggtga ccgttctgaa
     7441 ctaaaataag gagtacgaac catgacacta aaaatctttt tagaagtagt tgaaatacga
     7501 acaaaggtgg ctagtgtatt tccttttgcg gtaggggtat tattctctat cgcttttttt
     7561 catgagatac attggctaaa cactttttta ttttttattg gaatgctagt ttttgattta
     7621 gcgactaccg ctatcaataa ttacatggat tatgaaaagg ctcattctga agtatataag
     7681 tatgaagaaa atgtcattgg ccgcagaaat ctatctcctg tattgattcg aaacatgatt
     7741 ttagctatga ttgcatttgt ttttgttatc ggcgttattc taacgatcaa tacgggttgg
     7801 cttttacttt tgatgggatt tatctgttgt tttattggag tattctatac attcggaccg
     7861 attcctttat ctcgaatgcc tttaggggaa attttcagtg gggtgactat gggccttgga
     7921 atttttgcca tggtcgttta tgtcaataca ttcggttcag atgtctatga tctgacagtc
     7981 aacttttcat ctggacaatt tgctttggta ggaaatcttt gggctatagc agcaatcgta
     8041 ttagcttcct tacccttggt gtttacgatt gccaatatca tgttggctaa taatttgcgg
     8101 gatcttgacc gtgatattga aaatcatcgc tatacactag ttttttatat cggacgtcct
     8161 gcgggaacag tcttattcca gctattgatg tatgcttgct atttcgtgat tttaatgggg
     8221 ttgttgagcg gtatctatga atggccgatc ttggtcacct tcttgacctt gccaattgtg
     8281 aagaagcatt tggatcaatt caagcaaacg ttaccccagc ctatcagttt cagctattcc
     8341 atcaaaaata tggtcttatt caatgccagt tatgtattgg ggctattact aacaatcatt
     8401 atgcaagcca tttaacagca tgaagaggtt gtgaaaaaac tgccctgcat caagtaataa
     8461 gaacagccaa ataaaaatag ctccttatat ttttcgtttg gttgggctta ttaccgcaga
     8521 tgcaagcttt tgaacaccgt ttatcaggag gttgtgaaaa agtcgcttag tttcgagtat
     8581 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
     8641 gaaactggct tttgaacacc gtttatcaga ggttgtgaaa aagtcgctta gtttcgagta
     8701 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
     8761 agaaactggc ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt
     8821 attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg
     8881 aaggacagct gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt
     8941 ttcacagtcc cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct
     9001 tggtttggta actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta
     9061 tacaaagtca cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat
     9121 cgataccaat attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag
     9181 ttacgaagat aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag
     9241 caatcttaca taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg
     9301 ttttacatgg tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg
     9361 ttacgaattc ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag
     9421 ttgcacccat cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa
     9481 tagcatgtaa gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt
     9541 tttggcgata atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca
     9601 attcattttc gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg
     9661 gtggatgcgt aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc
     9721 cattaaataa acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta
     9781 atttaccgat cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat
     9841 ctactttgat aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt
     9901 cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat
     9961 tttcaaatag caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg
    10021 cgtaagtttt tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat
    10081 caaacgttct aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac
    10141 gaaatacacc tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag
    10201 gtaactctgt taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg
    10261 tcattttact tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac
    10321 cgtttacttc gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat
    10381 gattgctatc taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc
    10441 tttctactag accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg
    10501 catctcgttc aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac
    10561 aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc
    10621 catgcatccc tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt
    10681 aatcatatat ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa
    10741 cgatcctttt tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg
    10801 cttaaaacag ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt
    10861 ttcaccggct gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg
    10921 gattcgcctt cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga
    10981 ccataaaatt cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc
    11041 tgtgttgcct catcataaga taatacttca ccgacaaatt tatatactgg gattttacga
    11101 cgttcaccga ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat
    11161 tctcgttgtg ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca
    11221 ggatcttcca tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa
    11281 tggatcgatt tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct
    11341 ggaatatgtt cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg
    11401 cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc
    11461 cagcgacaag attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac
    11521 aatgtgcatc gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg
    11581 gtatccgtat ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg
    11641 acacgttcca acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct
    11701 tgagtggata aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct
    11761 ggatcagaaa caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct
    11821 gctccatctt catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca
    11881 tgttcttttg caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg
    11941 cttcttaaac catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg
    12001 gtttttaact tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt
    12061 tttactgtca tttctttcat catactcctt atttgatagc agaaggatca atataaaaga
    12121 atcctgtatc taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt
    12181 ccgctttttc ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc
    12241 gagtgatgtt tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt
    12301 aatgatgctg ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa
    12361 tatgtgtgcc atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg
    12421 agataaaaag acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat
    12481 tctgtagcag tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta
    12541 caggaatatc gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa
    12601 gtactgctcc aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta
    12661 atgtttcagc atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa
    12721 taatacctgt atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat
    12781 attcaggtac atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt
    12841 ttttaccata cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag
    12901 caggcagacg caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa
    12961 gcaactgttc tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct
    13021 cctcctacaa cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt
    13081 aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt
    13141 ccaaatataa aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact
    13201 attataaaaa ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta
    13261 ggtccgcgaa cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct
    13321 aaacgaattt ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt
    13381 gcgcgacgga ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg
    13441 attgcagatg gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct
    13501 ttgctcatga tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca
    13561 gtctcatcga ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata
    13621 cgtttttgca tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt
    13681 tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag
    13741 tccacttttc tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa
    13801 ttgaaaacgt ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt
    13861 tgacgaattt tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac
    13921 ccgattaatc ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg
    13981 gcatcttcac ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca
    14041 ccagttggtc caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt
    14101 cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt
    14161 tcatgcagaa gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt
    14221 agagggacac cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca
    14281 gaataacctt gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt
    14341 gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct
    14401 tccttttctt gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga
    14461 tcggtttgat ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg
    14521 ggtagttgac gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca
    14581 tctgtgatct ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact
    14641 tctgcttctt caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca
    14701 gaatcttttt cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat
    14761 tctcctcgag ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca
    14821 cctgcaccaa ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag
    14881 atttcgtcga tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca
    14941 accaaagatc ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca
    15001 cctaaaatga ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca
    15061 cccaccagta cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact
    15121 tctctcgaac gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca
    15181 cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt
    15241 acatttctac gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt
    15301 ttcagaactt gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc
    15361 atgatacttt catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa
    15421 ccagttttct tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat
    15481 ggaagaaaga ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt
    15541 tcttcataaa tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca
    15601 ttttgctcga ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac
    15661 cgttttgctt cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct
    15721 tccatctaat ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc
    15781 cgagcacgtg tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc
    15841 aacatcaagt ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata
    15901 gcaaaagcat ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat
    15961 tgttttttat ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg
    16021 ctttctacta gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt
    16081 gaaggaacac agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta
    16141 ttttccagaa ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc
    16201 ttattcattc cctttgacta atattgacct taattataac gaatttgata atagaaagaa
    16261 agaagaacgc tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata
    16321 ctattccgta tcgacgcgcc caagaggagt cgaaccccta accttttgat ccgtagtcaa
    16381 acactctatc cagttgagct atgggcgcat attcaattta aaaaaattat aaatcatttg
    16441 aaaacaaaaa gcaagataaa aaaaatcggg aagacaggat tcgaacctgc gaccccttgg
    16501 tcccaaacca agtgctctac caagctgagc tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DACDHL010000053        16334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDHL010000053 DACDHL010000000
VERSION     DACDHL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.053"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..67)
                     /locus_tag="IV228_001830"
     CDS             complement(<1..67)
                     /locus_tag="IV228_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8717719.1"
                     /translation="MKHLKILSIKETQLIYGGTTHS"
     gene            complement(461..2188)
                     /locus_tag="IV228_001831"
     CDS             complement(461..2188)
                     /locus_tag="IV228_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8717720.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2181..3374)
                     /locus_tag="IV228_001832"
     CDS             complement(2181..3374)
                     /locus_tag="IV228_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8717721.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3561..4205)
                     /locus_tag="IV228_001833"
     CDS             complement(3561..4205)
                     /locus_tag="IV228_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8717722.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4299..4433)
                     /locus_tag="IV228_001834"
     CDS             complement(4299..4433)
                     /locus_tag="IV228_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8717723.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4435..6567)
                     /gene="feoB"
                     /locus_tag="IV228_001835"
     CDS             complement(4435..6567)
                     /gene="feoB"
                     /locus_tag="IV228_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8717724.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6564..7037)
                     /locus_tag="IV228_001836"
     CDS             complement(6564..7037)
                     /locus_tag="IV228_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8717725.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7438..7662
                     /gene="nrdH"
                     /locus_tag="IV228_001837"
     CDS             7438..7662
                     /gene="nrdH"
                     /locus_tag="IV228_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8717726.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7821..9980
                     /gene="nrdE"
                     /locus_tag="IV228_001838"
     CDS             7821..9980
                     /gene="nrdE"
                     /locus_tag="IV228_001838"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8717727.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10030..10995
                     /gene="nrdF"
                     /locus_tag="IV228_001839"
     CDS             10030..10995
                     /gene="nrdF"
                     /locus_tag="IV228_001839"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8717728.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11084..12223)
                     /locus_tag="IV228_001840"
     CDS             complement(11084..12223)
                     /locus_tag="IV228_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8717729.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12532..13245)
                     /locus_tag="IV228_001841"
     CDS             complement(12532..13245)
                     /locus_tag="IV228_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8717730.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13499..14200)
                     /gene="nagB"
                     /locus_tag="IV228_001842"
     CDS             complement(13499..14200)
                     /gene="nagB"
                     /locus_tag="IV228_001842"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8717731.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14434..15966
                     /locus_tag="IV228_001843"
     CDS             14434..15966
                     /locus_tag="IV228_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8717732.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16157..>16334)
                     /locus_tag="IV228_001844"
     CDS             complement(16157..>16334)
                     /locus_tag="IV228_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8717733.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5068 a         3370 c         2845 g         5051 t
ORIGIN      
        1 cactatgagt ggtaccccca tagataagtt gtgtctcttt aatagacaaa atttttaaat
       61 gtttcataat ctaatcaccc ctaaattaat gtttttagac tattattcta acattcatta
      121 aatttcaaaa aacaattcct tcttgaaacg tagcaaaata attcctgaat aagaatttaa
      181 tcacttttaa gtatataaac tatgtatatt ctttaatatt ttttgagaat gcacttcgta
      241 cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt tgactcgcac
      301 ccataaattc ataatagttt gtcggcgtct ttgaacgttt cgaagatgag ctacgcattc
      361 tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt tggatatttg
      421 atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc cagctttcga
      481 ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct tttccattgt
      541 tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg tctaatactt
      601 tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg atctgctctt
      661 cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg gcaaacatcc
      721 aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct tgacctagtg
      781 caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag ttgtcatatt
      841 tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt cctatgattt
      901 tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag gctggcactg
      961 ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga tctttatatt
     1021 gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca tctgactcgc
     1081 catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt acatcatagc
     1141 ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta tcccattcaa
     1201 cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct ataccaatcg
     1261 cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt ttacttttct
     1321 gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt gtaaaacggt
     1381 caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc ccgacttgtg
     1441 ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc atagaggcaa
     1501 taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg atcgtacttt
     1561 tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg aatgaatcag
     1621 aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa cgaacggttg
     1681 gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata ccgaaaaatg
     1741 caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca aggatcggtt
     1801 tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt accccaatga
     1861 tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa tcattccata
     1921 gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt cctggacgtt
     1981 tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga gctggaatca
     2041 ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt aaaaatgaaa
     2101 ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag tttgccactg
     2161 gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc tgctaatgct
     2221 tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac aactgcaagt
     2281 ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc tttcccaatc
     2341 accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct agcttctaac
     2401 gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc catcagcatg
     2461 ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga ttcgatattc
     2521 gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg atcgacttct
     2581 tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc acctgttccg
     2641 atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg taaagcttca
     2701 ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag gtccagtaat
     2761 tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc gtccatcagc
     2821 aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg catcccacca
     2881 gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc taatgctttt
     2941 tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc gtattctgta
     3001 ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat attgatttta
     3061 tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc tccatcaata
     3121 tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa tgtggatttc
     3181 ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc aaaattcacg
     3241 tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg ctggtgatca
     3301 ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa atgttttact
     3361 tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt tctgaaaaaa
     3421 gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga aatttgcttg
     3481 tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc aagttaatat
     3541 ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa aaagtttgtc
     3601 atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc cgtaaattta
     3661 gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat aactgtggcg
     3721 ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat tgctttttct
     3781 gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt cgtttgattg
     3841 accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt ctccacactt
     3901 gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg caataggaaa
     3961 tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac ttccatgatt
     4021 ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc attgtacgta
     4081 ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt ttcaacgata
     4141 cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt tttctttcca
     4201 gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct ctagcaaaat
     4261 aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat ctgttccttt
     4321 tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa acgagtatag
     4381 acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc catcctattc
     4441 ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct gacggaacaa
     4501 aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg ctacctgtcc
     4561 accctccaat aagtgtccca actgataaat cacaaaacta actaaataag ctaaaccgca
     4621 ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat ggatagcccc
     4681 aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat aagctgctgc
     4741 gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg atacttccgc
     4801 atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca atcctgtaat
     4861 cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa ctgccaggat
     4921 tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat aaaaattata
     4981 agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg cttttttaac
     5041 aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag gaaaatgata
     5101 tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg tttttttcaa
     5161 gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag gagctaccca
     5221 agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact ttgcagaaca
     5281 aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt caatcgtgcg
     5341 actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa tgaaggattt
     5401 tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc tcgccatata
     5461 tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg gtagaaatcc
     5521 taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca tccatcccgc
     5581 tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac caaacaatgt
     5641 gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat aatagatcag
     5701 ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa tctgatcgat
     5761 tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg tacatagttg
     5821 ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt ccaatagttt
     5881 ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta tctcgtaatc
     5941 ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt tcttgccgct
     6001 ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg gatcatatgt
     6061 acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt cgattagttt
     6121 ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa gaccatagga
     6181 aagcttttct agattgattt tttttccttc ttttttgatg acatccatca tattcagacc
     6241 aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat ttcgttctaa
     6301 attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca aataatctct
     6361 tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta ggtcctgaat
     6421 atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta cgcccggcca
     6481 attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt tcccgctgtt
     6541 tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa gattagttcg
     6601 gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat tggatctccc
     6661 agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat atccattaac
     6721 cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc tcctgctttt
     6781 aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac aaagatttga
     6841 cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat ggcattgttg
     6901 cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt ttgcaaatgg
     6961 cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt ctgctttgcc
     7021 tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat taaaatcatt
     7081 cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat tctcacatag
     7141 aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg aatctttagt
     7201 attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat tttttctcta
     7261 ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa aaacacaaaa
     7321 cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga cttttttact
     7381 aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc actcaccatg
     7441 aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa acggttttta
     7501 acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga agcagtagac
     7561 tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc agaaacagtt
     7621 atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta aaagaatcaa
     7681 aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc aaacttccaa
     7741 atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca tcaaatacga
     7801 aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt tatttcaagc
     7861 tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag gataaagaag
     7921 ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt ccatctatta
     7981 tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc attcaattgt
     8041 attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat ttttctttca
     8101 aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc aacgacggtt
     8161 ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac tttgcgaacg
     8221 gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga tatcagccag
     8281 ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg tcttgtttct
     8341 tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct gctttgcaat
     8401 tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa gccggcgctc
     8461 ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa ttattcgaag
     8521 atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg gtttatttga
     8581 acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat gcagatgaaa
     8641 agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt tatgaattga
     8701 cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa gaatatggtg
     8761 tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca aatccaaaca
     8821 ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa ttacaacaag
     8881 aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat ccaatcgatg
     8941 gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg ccttctgtga
     9001 ttaacggcaa acaggaatat gaagtattag gaacagatat ctcatgtaac ttaggctcga
     9061 caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt acgatgactc
     9121 gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg atccaaaacg
     9181 gaaataagct gaatcatacg attggattag gtgccatggg cttacacact tatcttgcaa
     9241 aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt tacttcatgc
     9301 tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga aaacaagttt
     9361 tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa tacgtaactg
     9421 gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt tttatcccaa
     9481 ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg tatcatcaaa
     9541 atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg agtgcaagta
     9601 ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt aaaatttatt
     9661 acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct tatgatatgg
     9721 acatgcgtaa agtaatcgat gtttatgcaa cggcacaaca acacgtcgat caaggcatga
     9781 gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg aagacaacaa
     9841 cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac aaaggaatca
     9901 aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga agcaatcaat
     9961 gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt taattaaagg
    10021 agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat tgaagatatc
    10081 attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac acgtatccca
    10141 ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga tacagtagga
    10201 catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg catggatcag
    10261 ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat tcaattcatg
    10321 gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac gaaaaaagaa
    10381 atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa agctgagcgt
    10441 atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag cgtcttttta
    10501 gaaacattct tgttctattc aggtttttat actcctcttt attatttagg aaacaacaaa
    10561 ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt acatggaacc
    10621 tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga acaagaaacg
    10681 ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga acgttataca
    10741 gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct acgctataat
    10801 gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac tgccaatgat
    10861 gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga cttcttctca
    10921 caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga cgattatttg
    10981 attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata aaaagagttt
    11041 agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca cttgtttctt
    11101 ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc aataaataga
    11161 ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg caaatcagca
    11221 tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc gtcaaaggat
    11281 ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct aattgttgga
    11341 tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac aaaccgacaa
    11401 tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc gtgattccgc
    11461 aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc aaaaagctca
    11521 ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat aatgtttcgt
    11581 ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag aagtgagaca
    11641 acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat ggaacggtaa
    11701 ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt gcagataaac
    11761 tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct aattgttctt
    11821 ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat ttgcttgttt
    11881 catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt aactgaggca
    11941 ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc aataaaaaac
    12001 cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg aacatataat
    12061 agataaaacc agcaacaagc aatggtaaaa aaattgccga aagcattttc aatattggac
    12121 taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt actagctcca
    12181 ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc ctccaaggtc
    12241 ctttgatact tctattctat catttaacaa agagttacgc gctgagattt tcatttgctt
    12301 aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa acaaaaacac
    12361 ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa cggtgttcaa
    12421 aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact atttttattt
    12481 ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca tttattgcgc
    12541 aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct cgatcacatc
    12601 ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca ctcggtagtc
    12661 atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct cttcaaaata
    12721 atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta actttttagt
    12781 cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc cgataaaact
    12841 gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag caattgccca
    12901 taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga tcgttcccgc
    12961 gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat ctgaacgaat
    13021 cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag cgatctcaac
    13081 ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac gcatacctgc
    13141 tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca tagcgatccc
    13201 taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa caccctccag
    13261 tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac ctcttacgaa
    13321 aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg tgttcaatca
    13381 gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt tcctttgtct
    13441 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact attgaaaatt
    13501 acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga ttttgcaata
    13561 cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt gcatcagctt
    13621 tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa ccgattccca
    13681 ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg tttgcattga
    13741 ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca ccaggttcat
    13801 tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt ggatgtgcat
    13861 cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt ccatctggta
    13921 caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa tgacggtagc
    13981 tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa gtcatttcac
    14041 tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg cttcctgttg
    14101 ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca aatgcttttt
    14161 ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct cctttatatt
    14221 ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt atagaccaaa
    14281 ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt cataagatgg
    14341 ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt ggacgcttta
    14401 tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt aaacaacatt
    14461 acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt aaatgctggc
    14521 gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat taaaatcatc
    14581 actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat tcacaaaggt
    14641 tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt tctaaaaact
    14701 gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca aacatatagc
    14761 gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga attagaccaa
    14821 ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt ggggatcgat
    14881 gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc aaaggtgatt
    14941 ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc aaccaatcac
    15001 ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga cggtgcgtat
    15061 atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat cgcagacatc
    15121 gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa acaaaaggaa
    15181 caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat cgaaaagacc
    15241 gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa aagccgacaa
    15301 aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa agctgtcttt
    15361 gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga tttagtcatc
    15421 ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg tgaaaatatt
    15481 gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact gatcggtgag
    15541 attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa ttacttttca
    15601 caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag cagtcaattt
    15661 ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt agttaatcaa
    15721 ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt gaaacttgca
    15781 cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa ccatatcgat
    15841 caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac agtaatcatc
    15901 gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga gattgaacca
    15961 aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt ttgtcacaac
    16021 tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct caaaaaacgg
    16081 caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag aacagctttt
    16141 cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta aaaacaactg
    16201 tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca cctaaccagc
    16261 catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca ataaagtaca
    16321 taaacataaa taca
//
LOCUS       DACDHL010000054        15748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDHL010000054 DACDHL010000000
VERSION     DACDHL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.054"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..776
                     /locus_tag="IV228_001845"
     CDS             24..776
                     /locus_tag="IV228_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8717734.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            773..1687
                     /gene="pfkB"
                     /locus_tag="IV228_001846"
     CDS             773..1687
                     /gene="pfkB"
                     /locus_tag="IV228_001846"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8717735.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1705..3636
                     /locus_tag="IV228_001847"
     CDS             1705..3636
                     /locus_tag="IV228_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8717736.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3747..5306)
                     /locus_tag="IV228_001848"
     CDS             complement(3747..5306)
                     /locus_tag="IV228_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8717737.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5461..7695
                     /gene="pcrA"
                     /locus_tag="IV228_001849"
     CDS             5461..7695
                     /gene="pcrA"
                     /locus_tag="IV228_001849"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8717738.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7712..9748
                     /gene="ligA"
                     /locus_tag="IV228_001850"
     CDS             7712..9748
                     /gene="ligA"
                     /locus_tag="IV228_001850"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8717739.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9854..10159
                     /gene="gatC"
                     /locus_tag="IV228_001851"
     CDS             9854..10159
                     /gene="gatC"
                     /locus_tag="IV228_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8717740.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10159..11631
                     /gene="gatA"
                     /locus_tag="IV228_001852"
     CDS             10159..11631
                     /gene="gatA"
                     /locus_tag="IV228_001852"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8717741.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11628..13058
                     /gene="gatB"
                     /locus_tag="IV228_001853"
     CDS             11628..13058
                     /gene="gatB"
                     /locus_tag="IV228_001853"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8717742.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13068..14123
                     /locus_tag="IV228_001854"
     CDS             13068..14123
                     /locus_tag="IV228_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8717743.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14234..15607
                     /gene="rlmD"
                     /locus_tag="IV228_001855"
     CDS             14234..15607
                     /gene="rlmD"
                     /locus_tag="IV228_001855"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8717744.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15610..15738
                     /locus_tag="IV228_001856"
     CDS             15610..15738
                     /locus_tag="IV228_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8717745.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5358 a         2893 c         3461 g         4036 t
ORIGIN      
        1 tttgtattga ggtgatcgtg aaaatgctta cagaggaacg tagacaaaaa atcctagact
       61 tgcttgaaca gcagaaaatc gtgaaatcac aagatcttgt aaaccttttt aatgcttctg
      121 aatcaacgat aagacgtgat ttacaagagc tggagaatag tgggtttcta gaaagagttc
      181 atggcggagc taagaaagag caattattga attttgagca gaatatgagt gaaaaatcac
      241 tcaaaaacgt tcacgaaaaa cagcaaatcg ccaaatcggc agctgaaacc attaatgacg
      301 aagatgtcat ttatttagac gctggctcta ctacgttaga aatgattcct tttctgcaag
      361 gaaaacagat cacagtagtg acaaattccg ttcaacatgc ggcaaattta gttgatttgg
      421 agcttgctac cattatttta ggtggaacga taaagctggc aactaatgct gttttaggat
      481 ctaatgcgat agaacaacta aaaaattttc attttaataa agcatttatg ggaatgaacg
      541 gtgcccatct tgaacaagga ttcacgactc cggacccaga agaggcagct gttaaacgtt
      601 tagccatgaa aaattccgaa gagacatttg tattgattga tcataccaaa ttcaaccagt
      661 tgtccttcac ctctgttgca cctttggaag cagccacgat cataaccgag cgttgtccat
      721 tagaattcgt taatgaattc cgtgaaaaga caacaattaa ggaggcaata caatgattta
      781 cacagtaaca cttaatccat caattgatta tatcgtacgc gtagatgggt taaaactagg
      841 cgatttgaac cgaatgaagg aagacttcaa gttacctgga ggaaaaggga tcaatgtttc
      901 ccgtattttg aaacggatcg attctcaatc tacagcacta gggtttttag gagggttcac
      961 tggcgaattt atttcgaagt ggcttgaaaa agaacaaatc cagtctttgt tcacaaccgt
     1021 aaaagaagat acaagaatca atatcaaact aaaatcagat actgaaactg aaattaacgg
     1081 tttgggccca tctatttcag atgaagaaat cgatgagttg aagcaagcat taggaacagt
     1141 aggacaagga gatatcgtcg tattgtcagg aagcgcgccg gctagcctgc gaaaaggatt
     1201 ttatcaagaa ctgattgata tcattcgaga aaaaggtgca gatttcgtga ttgatacaac
     1261 aggtgatgac ctaaaaaatg cacttgttaa gaaaccattg ttgatcaaac caaacaatca
     1321 cgaattagct gagctttaca atacaacctt ccaatctgta gaagaaatca ttccgtatgg
     1381 taaaaaattg ctagctgaag gggcacagaa tgtgctgatc tcaatggctg gagacggtgc
     1441 tttgctcttc acggaacaag gaacttataa aacgaatgta ttagttcgcc cattgaaaaa
     1501 ttcagtaggt gcaggggatt caatgattgc cggctttatt ggggcttaca gccgtacgaa
     1561 agatccaatc gaagccttta aatggggagt tgcttgcgga agcgcaacag ctttttctga
     1621 tgacttggca gctcgcgaat tgattgatga gttgctgcca gaagttgaaa tcacaaaaat
     1681 tgattagttt caaggaggat aatcatgaat atcaaggatt taatggtcaa agaagcgatg
     1741 atcatggatt tgcaagcgac tgataaaaaa ggcgcaatcg atgaaatggt ccaaaaaatg
     1801 tttgatgccg gccggatttc cgatatcgaa acatataaag aaggaattct agcccgcgaa
     1861 gcacaaactt ctacaggtct tggtgatgga atcgccatgc ctcacgcgaa aaacagtgca
     1921 gttaaagaag caacagtttt attcgcaaaa agccaaaaag gagtagatta tgaagcctta
     1981 gacggacagc caacttatct gtttttcatg atcgcagctc cagaaggcgc aaacgacaca
     2041 catttgcaag cattagcagc actatctcga ttgctgattg atgcagattt cgtaggaaaa
     2101 ttaaaagaag caaatacacc agaagaagtc caagacttgt tccaaacagc tgaagagcaa
     2161 aaagaagcag aagaaaaagc agaacaagaa acacaagctg cagaagcttc tgctcatcca
     2221 gaaagaaaat tcgttgttgc cgttacagct tgtccaacag ggatcgctca cacttacatg
     2281 gcagaagatg ctctgaagaa aaaagcgaaa gaaatgggtg tggagatcaa agtcgaaacg
     2341 aacggttcag aaggaatcaa aaaccgattg actgctgaag acatcgcccg tgcagaaggt
     2401 gtaatcgttg cagcagacaa aaaagtagag atgaatcgct ttgacggaaa agaactgatc
     2461 aatcgtcctg tcagtgatgg tatccgtaaa ccagaagagc tgatcaatct agcccttagt
     2521 ggtactgcac cagtattcca tggtgatgga aaagaaagca gttcagaaga aggaaatgcg
     2581 gatggtacta tcggacaaag aatttacaaa gatttgatga acggcgtatc acacatgtta
     2641 cctttcgtta ttggtggagg aatcgctatt gcgctttctt tcatgatcga ccaatttatt
     2701 ggcgtgccac atgatcaatt atcaaatcta gggaattaca atcaagctgc tagctggttc
     2761 aaccaaatcg gacaagctgc atttggtttc atgctgccgg tattagcagg atttattgct
     2821 tcaagtatcg gtgaccgccc tggtttgatc gttggatttg ctgctggtgc attagctaat
     2881 acaggcggtg caggattctt aggtgcactg attggtggat tcttagctgg ttatgtcatc
     2941 gtatttttac gtaaattatt caagaactta ccaaaatcat tggatggaat taaaacgatc
     3001 ctcttttatc cagtgtttgg cttactgatt actggtttct tgatgttgct ggtcaatgtc
     3061 cctatgaaag caatcaatga tgcattgaac ggtttcttga caagtatgag tggttcgaat
     3121 gctgctttac ttggtgcatt attagctgga atgatggcgg cagatttagg cggtccaatc
     3181 aacaaagccg cttatgtgtt tggtacagca actttagcta cgactgttgc aactggcgga
     3241 agtgtcgtga tggcttctgt tatggctgga ggaatggtac ctccattggc aattttcgtt
     3301 gctacacgtt tgtttaagaa taaattcagc aaaacagatc aagatgctgg tttgacgaac
     3361 atcgtgatgg gactatcgtt tgtcacggaa ggatcgattc catttgcagc agctgacccg
     3421 attcgtgcca ttccaagttt tattattggt tctgctttaa ctggcggatt agtcggagct
     3481 ttcgggatca aattactggc tcctcatggc gggattttcg ttgtattctt actgagtcac
     3541 ccggtcatgt atttggtgtt tattgctatc ggagcagtcg tatctggtgt gatttatggt
     3601 ttattgagaa aagctcctgc agcagcagaa gcatagaaat aaaaaagaag aggctggttt
     3661 ttcccagtct cttctttttt tatagaatca tatagaatca agtagtgtag atgaatagtt
     3721 gttgtaaaat agtttttcca tgttcgttaa aatgattgct tcaaatttgt gatagaagat
     3781 ttagcctcga tttcctgctt gattcttggc aagtttgctt catcagtcaa gataaccaaa
     3841 atatctccta cattcatgat cgtatcaccg tgagtcaaga tttcctgatt tcctctgcgt
     3901 atagagatta gcagcatatt tttaggccaa ttgaagtctc gtaccattaa tccgtctaaa
     3961 gaactctctg ccgttactgg aaaatcaaaa gttgtccttg caccttttag tgaggcggtc
     4021 tgagtatttc caagtagacg ttccaataat gattcgtaaa ttggattacc gccgagaagg
     4081 tcattgacga tataagcaac aagagcagcc aatcctaaag gcatcagatg attaagagag
     4141 ccaaccattt ctgtcactaa aataattgct gtcagcggtg cttttccaat cgctgtgaaa
     4201 taaccagcca tggcaataac gacaaaatcc ttgatgtaga ttggatccat cccgaaatca
     4261 accataatgt ttccataaaa gctaccgatt aaagccccca atgtcaatat cggaaggaaa
     4321 atcccccctg gaagaccacc accgtaagaa atcatggaaa aaacgaatcg aagaacaaac
     4381 aaaccgataa tcataatgat tggcaaggaa gcatgtccaa aagctaaaac gatttgattg
     4441 cctccgccta gagtactagg ccataaataa ccaattggca aaatcagcaa gaaaggaacg
     4501 aacccgtgaa aataagctgg taaaggcaat cggttgtaaa attttggcaa agccagcaag
     4561 accttctgat aaataaaacc taagatgcct agaagtactc ctaagatcac taaggaacca
     4621 taatatttca acggcaaaga aggaaggtca cctagatata aaacaggtct caagccgaaa
     4681 aagtaaagag agacgaagtt agcagtgatt gctgaagcaa atgatgtcag ccaaacaagt
     4741 ggagaaaagg agtgatggat ttcttctaaa acaaagagta atcctgcgat aggagcattg
     4801 aatgctgctg caagtccgct acttgcaccg ctagaaatta aaatcttttc ttctgaagat
     4861 ggactccgaa acaattgtcc agctccttgt ccaatggatg cgccaagctg tatagaaggt
     4921 ccttctctac ctagaaaaag gcccgcacca actgaaagta caccgccaat gaactttttc
     4981 cataaaacag agaaccagtt cattgataat tccccgcgaa gctgtccctc tacttgtgga
     5041 atcccgcttc ccttgatatc cggttcattt ttgaccagat aacccacgaa aaatgctgca
     5101 aggatgcagc aaatagctag gccaattaag taaatcggct gttcccgact catttcatag
     5161 atacttcgaa ctaaatcgct tagcttttcg atagataatc gaaacaggct aacgacgatt
     5221 cctgtcagca gtccaacaaa tattcctttt agaataaaat atatctgagt cttatctaat
     5281 ttcttgattt ttcctttttt ttccattcaa acgcctcgct ttcttttaat ttagcgcgaa
     5341 atcaacgaat tgacaaggaa aaatccataa aaagaaacaa atgattttga actagctagt
     5401 ttaatgaaag ccaagaaaat gctataatta acaagattga taaaagaaag gatgttgtta
     5461 atgtcatcca aagctgaatt attaaacggg atgaaccccc gtcagaaaga agctgttctt
     5521 cacactgacg gaccattact gctaatggca ggtgcaggaa gtggaaagac gagagtgctg
     5581 acacaccgca ttgcctattt gattgaggaa aaagaagtca atccttggaa catcctagcg
     5641 attactttta ccaataaggc tgcaaaagaa atgaaagaac gtgtcaatgc gattctcgca
     5701 tctggaggcg aagatgtctg ggtatcgact tttcactcga tgtgtgtgcg tattttaaga
     5761 agagatgttg attttatcgg gtataaccgg aactttacaa tcatcgattc ttctgaacag
     5821 ctgaccttga tgaaacggat actaaaagaa ctgaacattg atccgaaaaa atatgatcct
     5881 cgaagtatat taggaacaat ctctcaagca aaaaacagcc tgcagacccc tcaggatttt
     5941 gcaaaaatgc aaggcagtta ttacgaagag attgcggcaa aatgttatgc tgcttaccaa
     6001 aaagagcttc aatataatca atgtatggac tttgacgacc tgatcatgaa tacgattcga
     6061 ttatttgaag aacatcctga ttcactcaca tattatcaaa acaaattcca ttatattcat
     6121 gtagatgagt accaagatac aaatcacgca cagtatacgc tagtcaattt attggcaggt
     6181 agatttagga acctgtgcgt cgtgggagat gcagatcaaa gtatttacgg ctggcgcggt
     6241 gcagatatgc agaacatctt ggattttgaa aaagattatc cagatgctgc agtaattttg
     6301 ctagaacaaa actatcgctc cacgaaaaac atccttagcg cagcaaacca agtcattgaa
     6361 aacaatagca atcgaaaacc aaaaaacttg tggacagaaa acaaagaagg aaacaagatc
     6421 acttactacc gtgcagataa tgaacgagac gaaacccgtt ttatcgtaga tcggatgcaa
     6481 gaagagattc gaagcaatca tcggaattac ggcgactttg ccatcttgta ccggacgaac
     6541 gcacaatctc gtgtaatgga agaaactctg ttgaaagcaa atattcctta taaaatggtt
     6601 ggagggcaca agttctacga tcgtaaagaa atcaaagata ttttagcgta tttgaatgtg
     6661 ctggcaaatc ctcaagattc aatcagtttt gagcgtatcg ttaattcgcc caaacgaggc
     6721 atcggaccag gatctatcga aaaactacgc agttttgctt ctcttcatga atggccactg
     6781 cttgaagcag cgcaaaatgt ggatctggca aatatcggtg gaaaagcagg acagcaatta
     6841 ggtgcatttg gagagatgat ccaagaagtc actcagatga tcccatactt gacagtgaca
     6901 gaattgacga aagaggtttt ggatcgtagc ggatatctag aagacttaaa aatccaaaat
     6961 acattagaag cacaagcacg catcgaaaac ttggaggaat ttttaacggt cacacaagaa
     7021 ttcgacaaac aattcgaaca gcaaaatgaa gaagacgctg atgcaccaga agaaaaacta
     7081 acggtcttct taaatgatct ggcacttgtt tccgatattg acaatttaga agaagatgct
     7141 tcccaagtga cattgatgac tttgcatgca gccaaaggat tagaattccc agttgtattc
     7201 ctgattggtc tagaagaggg ggtttttcct ttatctcgag cattgatgga agaaagcgaa
     7261 ctggaagaag aacgccgtct cgcatatgtg gggatcacgc gagcagaaga agcgttgtat
     7321 ttaacaaatg ctttctccag aacattatat ggacggacgc aatataaccg accttctcgt
     7381 tttgtagaag aaatcgatca ggagttactg gaaatcgaag gcatgcgtcc tacgcctaaa
     7441 aaaacacctg tattcgctaa aaaaacagca tatagctata aacagccaga gacagcggtt
     7501 gttccctcta aaagtgctac tggaggggaa aataacagct ggaaacctgg agataaagtc
     7561 aaacacaaaa aatggggtct aggaacagtc gtccgagtaa gcgggacgtc gaaggatcta
     7621 gaactagatg tggctttccc aagtcaagga gtaaaacgtc ttttggctgc ctttgcgcca
     7681 attgaaaaag cttagtagtg gagggtaaat gatggaacat gaaatgacga ttgacgaagc
     7741 tgctaaacga gcagaagaac ttcgtacaag actgaaccaa tggtctcgcg aatactacgt
     7801 agaagataaa ccaacagtgg aagactatgt ttatgataaa gaatacgcag aattagtagc
     7861 aattgaggaa caatatcctg atttgatcac gtctgattct cccactcaac gagtaggcgg
     7921 aaaagtatta gaaggttttg aaaaagtcac tcatgatatt ccgctttaca gtttgaacga
     7981 cgttttcagc aaagaagagc tgatagcttt tgatcaacgt gtacaaaaag cagtgggacg
     8041 tgtagttgac tattgctgtg aactaaaaat agatggactt tccgtttctt tgcgttatga
     8101 agatggaaat ttcgtgagag gtgcgactcg cggggatgga actgtaggag aaaacatcac
     8161 tgaaaaccta aaaactgtcc ggtcggtccc aatcaaatta aaagaaccaa tgaacatcga
     8221 agtaagaggc gaatgtttca tgccaaaacg gtcatttgtc caattgaacc aagacagaga
     8281 agcagaggga aaagatatct ttgcgaatcc tcggaacgcc gcagctggtt ctttgcgtca
     8341 gttagattct aaaatcacag cgaaaagaaa tctggacact tttttataca cagtagctga
     8401 tttcggtcct atgcaagcaa aaacccagta tgatgcgcta gaagagctag aaaaaattgg
     8461 attccacacg aatcgggaaa aacgtctatg tcattcgatt gatgaagtgt ggtcatacat
     8521 tgaagaatat catgacaaac gtgtggattt gccctatgaa atcgatggaa tcgtcatcaa
     8581 agtgaatgaa ttctcgctac aagatcaact tggttttact gtcaaagcac cgagatgggc
     8641 agcagcttac aagtttccgc cagaagaagt tgaaacgcta atcgaaaaca tcgaatggac
     8701 agtcggtcga acaggggtag taacgccaac agctatcatg acaccagtcc gagtagcagg
     8761 aacgacagta agccgtgcca gcctgcataa cggtgattat attaagttga aggatatccg
     8821 cttgaaagat acagttctga tttataaagc gggagacatc atcccagaag tttcgcaagt
     8881 cgtgttagat aaacgaccta aagacagcga agaataccaa ttgcctacac attgtccagt
     8941 ttgtggaagt gaacttgtcc atttggatga agaagtagcc ttacggtgca tcaatcctaa
     9001 atgccctgct caaatgaaag aaggtttgaa ccactttgtt tcaagaaatg cgatgaatat
     9061 cgatgggttg gggccgcgcg tactagaaca gatgtatgat aaaaaactag tagcagatgt
     9121 tgctgatctt tacaaattga cagaagaaga gttattgacg ttggacaaaa taaaagaaaa
     9181 atcagcaaat aatattttga cagcaatcga caacagcaaa gataattcgg tcgagcggtt
     9241 gattttcggg ttaggaatcc gacatgtcgg agcgaaagca gcaaaaattt tagcagaaca
     9301 tttcggtgat ctagaaacat tgagtaaaag tgactatgag tcgattattg cgcttgatac
     9361 gatcggtgat atcattgccg acagtgtagt cacttatttc agtaacgaag aagtccatga
     9421 attgatgaat gaattaaaac aagcaggcgt caatttcgaa tacaaagggc ttcgaaatgc
     9481 ccaattgcaa gaagtcgagt ctccttttaa agaaaaaaca gtagtcctta ctggtaaact
     9541 aacacgattt actcgtgaag aagcaaaaga gacgatcgaa aatcttggcg ggaaagtgac
     9601 tggtagcgta tcgaaaaaaa cggatatcgt ggtagcagga gaagatgctg gcagcaagct
     9661 gaccaaagca caagaattag gaatcgaagt ttggacagaa gatcaaatgg cagatgcatt
     9721 ggctaaaagt cgctcagtag aagaatgaaa aatgagtttt ttcagacaaa tttcaataaa
     9781 caatttcaga cacggcactc ttgtgttaaa atagagagca aagtttttaa ctcgtttaga
     9841 aagaaggggt agtatggcga ttagtgaaga acaagtgaaa cacgttgcca aattagcaaa
     9901 acttgcgttc gcagatgaag aacttgcaag ttttaccgac caattaggta aaatcatcga
     9961 tatggttgaa atgctggaag aagtagatac agaaggtgtt ccatttacgt cgaatgtggt
    10021 tgaaacagta aacgtgatgc gacaagatcg ggcgcaagct ggatggaacc gcgatgaatt
    10081 attgaaaaat gtgccggaaa cagaagacgg ctttatcaaa gtacccgcaa tcatcgataa
    10141 tggggaggct ggcgcataat gacagaatta cacaatcgtt ctctagaaga actgcatcat
    10201 atgcttgtat cgaaagaaat cactgtacaa gatttgacaa aagcaacatt tgaacgaatc
    10261 aaagaaacag agcctgaggt cgaagcattc attacattga acgaagaaaa agcattggaa
    10321 caggcaaaag ctttagatga aaaagggatt gatgaaagca acgtccttgc tggtattcca
    10381 attggtatca aggataatat cgtaacaaaa gatatcttga caaccgctgc atctaagatc
    10441 ctttacaatt ttgaaccaat ttatgatgca acggtcatgg ataaaatcta tcaagcagat
    10501 atgatccctg ttggtaagct aaacatggat gaatttgcta tgggtggaag tacagaaact
    10561 tcttatttca agaaaacaaa aaatgcttgg gacccaacaa aagtacctgg tggctcttct
    10621 ggtggttctg cagcagcagt tgcagctgga caaattccag tttctttagg tagtgatact
    10681 gggggaagta tccgccaacc agcagctttt aacggcatcg tagggatgaa acctacttat
    10741 ggccgtgtct cacgttttgg tttgattgcc tttgcttcat ctcttgacca aatcggacca
    10801 atgacccgta cggtgaaaga taatgcatta gcattgacag ctatcagcgg ttatgacgaa
    10861 aaagacggta cttcttcagg cgtttcagtt cctgattttg cagagggact gacaggcgac
    10921 atcaaaggaa tgaagattgc tttacctaaa gaatacttag gcgaaggtgt ggcccctggc
    10981 gtaaaagaag ctgtattgaa agcagcagaa acattccgtt cattaggtgc tgttgtggaa
    11041 gaagtcagct tgcctcattc aaaatacggt gtggctgttt attatatcat tgcctcatca
    11101 gaagcaagtt ccaacttgca acgattcgat ggtatccgct acggttatcg ctcagaaaat
    11161 gtgaaatcat tagatgatgt atatgtcaac tcacgttcag aaggattcgg tgaagaagta
    11221 aaacgccgta tcatgttagg tacattctcc ttaagtgctg gttattatga cgcttacttc
    11281 aaaaaagcgg gacaagtccg tacattgatc aaacgagatt ttgaaaaagt ctttgcagat
    11341 tatgacttga tcatcgggcc atcttcacca acagttgcat tcggaatcgg tgagaacatt
    11401 aacgatccaa tcacaatgta catgagtgac atcttaacga tccctgtcaa cttggctgga
    11461 cttccaggta tgtctgtacc ggcaggttta tcagaaggat taccagttgg attgcagatc
    11521 atcggtaatt attttgatga aaaaacgatg tatcaagcgg catatgcgtt tgaacaagca
    11581 acagaattcc ataaacaaca accagctatt ttaggaggga aagacgaatg aattttgaaa
    11641 cagtcattgg actagaagtc cacgtcgagt taaaaaccaa ctcaaaaatt ttctctccag
    11701 cgccagctca ttttggtgca gaaccaaaca gcaatacaaa cgtgatcgac tggggttatc
    11761 caggcgtgct gccagtaatg aataaagctg cgctggaatt cgggatgaaa gcagctttgg
    11821 cattaaactg tgaaatctct aaagatactc attttgaccg gaaaaattac ttttacccag
    11881 ataatccaaa agcatatcaa atttcccaat ttgatcaacc aatcggtcat gacggatgga
    11941 tcgaaatcga agtagaaggt aaaaagaaaa aaatccggat cgaacgtgtc catttagaag
    12001 aagatgctgg aaaaaacatg catggtattg gcggttattc ttacgttgat ttgaaccgac
    12061 aaggcacgcc tttgatcgaa atcgtttctg aagctgatat gcgttcacca gaagaagcat
    12121 acgcttatct agaagcattg cgatcaatta tccaatttac agaagtcagc gatgtgaaaa
    12181 tggaagaagg ctctatgcgc tgtgatgcca atatttctct tcgcccttat ggacaagaag
    12241 agttcggtac gaaagcagaa ttgaaaaact tgaactcttt atctttcgtg aaaaaaggtt
    12301 tagcttttga agaaaaacgt caagcaaaag ttcttctttc aggaggagaa atccaacaag
    12361 aaacacgccg ttttgacgaa acaacgaata aaacagtttt gatgcgtgtc aaagaagggt
    12421 ctagtgatta tcgttacttc ccagagcctg atatcccgaa attcgtgatc gatgacgaat
    12481 ggattcaaaa agtaaaagct agtttgcctg agatgccagc ttctcgtcgc gaacgctata
    12541 tccgtgagtt tggacttcca gagtatgacg caatggtatt gacacttaca aaagaaatgt
    12601 ctgatttttt tgaagctgtt ttggaaaatg gtgcagatgc caagcaagca tctaactggt
    12661 tgatgggtga agtttctgct tacttgaaca gtgagaaact ggaattagct gagacaaaat
    12721 tgacacctga gaacttagca ggaatgattc gtttgatcgc cgatggaacg atcagctcaa
    12781 aaatcgccaa aaaagtcttc cgtgaattga tcacaaatgg cggagaagcc caagaagtag
    12841 ttgaaaaaaa tggttgggtg caattatctg atccgaacaa actactgcca atcattaatg
    12901 aaattttgga taacaaccaa caatctgtcg atgatttcaa aaatggaaaa gatcgtgcgg
    12961 ttggcttcct agtcggtcaa atcatgaaag caactaaagg acaagcaaat cctggtgtcg
    13021 tgaataagct acttaaagat gaattagcga aacgatagag gctgaaaatg aaaaaagcac
    13081 gcgtaattta taaccccacc tcgggaaaag aattattgaa aaaaaatttg gcggatatcc
    13141 tacaggcatt agaagaagca ggatttgaag ccagcgccta tgcaactaca ccagaaccag
    13201 attcagccaa gaatgaagca tgtagagtcg cagaactagg ttttgatcta gtagtggcag
    13261 ctggtggcga tggcacaatc aacgaagtgg ttaatggaat cgctccgctt gaacaccgtc
    13321 ctaaaatggc catcatccca gctggtacaa cgaacgacta tgctcgagca ttaaaaattc
    13381 ctagagataa tattaaagca gcagcagaag tgataaaaaa acaccaaacc gtaaaaatgg
    13441 acatcggcct ttctgataac agttacttca tcaatatcgc tgcaggtggc tatttgacag
    13501 aattgactta tgaagttcca tcagaactaa agagtatttt tggttatctt gcttatcttg
    13561 cgaaaggagc agagatgctc cctcgagtaa agccaatcaa aatgcgcttg aagtatgatg
    13621 aaggggaata tatcggaaat gcttcgatgt ttttcctagg gctgacgaac tctgttggag
    13681 gattcgaaca gatcgcgcca gatgccaaat tggatgatgg gaagttttct ttaatcattg
    13741 tgaagacagc aaatatcttt gagatcttgc atattgcagc actcatgctg aatggcggtc
    13801 gacatgtcga tgatccaaga ataatctata ccaaaacaag cagtttgttc gttgaaacag
    13861 aagatgatat caatcgacca gtaatgatca acttagatgg tgaatacggc ggggaagcac
    13921 cgatgcattt ccaaaatctt catcaacaca ttgagttctt tgccaatacc gatcagattc
    13981 ctgatgaagc aatcacaggt acagatgaag aagaattaga ggttgtcagc aaagaattcg
    14041 taaaagaagt cgaacgttta acggatgaag atatcgacgg tgacggaaaa atagcagacg
    14101 aaacacctaa tcaaaagaat taatcaaaag ccggtgctga acgtagtaag gtcagtgccg
    14161 gttttagttg tgtaattagc tgatttatag tagagttcag taggattgaa aaataggctt
    14221 aaaggagcaa aatatgacag aacagcaaat agtaaagaaa aacgaacgat atacagtcga
    14281 aatcgtcgac ttgagttatg aaggaatggg ggtagcaaaa atcgatggtt atccattatt
    14341 tatcgaaaat gcactccctg gagaagaagt agaaatacta gtagtaaaag caggcaataa
    14401 gtttggatat ggaaaagtcg aaacatttat tacacaatca cctgatcgtc agcctgtaga
    14461 aaacaatgca ttacttcgca taggtatcgc acctttagcc catttgaagt atgaacaaca
    14521 gcttgtcttc aaacaaaaac aagtagagca tgtaatggcg aagattgcta agatgccaga
    14581 agttcctgta cttccaacgg tcggaatggc agaaccattc ggctaccgta ataaagcaca
    14641 gatccctgtt cgccgtatcg atggacagtt agccaccggt ttttacaaaa agaatagcca
    14701 tgatctagtc cctatcgaag atttttatat ccaagatcct gtcatcgatc aagcaatcaa
    14761 agtagtcaga gatatcttgc aacggttcca agtacgagga tataatgaag aaaaaaatga
    14821 aggacagctt cgtcacatca ttatccgtcg tggtcattat tcccatgaaa tgatggtcgt
    14881 tttagttact cgtaaagaaa aattctttaa atccaaggaa attgctgaag ctattcatga
    14941 agaattgcca gaagtggttt cggtcatcca aaatatcaat gaagaaaaaa caaatgtgat
    15001 tttaggagac aaagaaaaag ttttatatgg cagagactat atcgaagatc agctattagg
    15061 aaaaacctat cgaatctcag caaagtcatt ttatcaagta aatactcaac aagcagagta
    15121 tctgtaccaa acagccatcg agtttgccga gttagataaa aaagacattg tgatcgacgc
    15181 ttattcagga ataggtacga ttggcttgtc tttagcagac agagtggata aggtgtatgg
    15241 gatggaagtt attccagaag caattgaaga tgcacagttc aatgctttaa ataataaaat
    15301 cgaaaatgtt cattatgaag tcggaaaagc agaaaaagtt atgaaaaaat ggcaagaaaa
    15361 agggatcaaa ccatctgtga tcgtagttga ccctccacga aaaggattag atcatcattt
    15421 tattgaatca gcaatcgaaa tgtcaccaga acgaatcgtc tatatctcat gtaatccggc
    15481 aacttttgca agagatgcta agttatttgc agaagcaggt tatgccgtga agaaagttca
    15541 gcctgtagat cttttcccgc aaacccatca tatcgaatta gtcgcttcat tcgttaaagg
    15601 agaataaaaa tggaccaaaa agaaaaaatg aaagaattga tcaaaaagaa acaaggtgga
    15661 ggccgatcca aacaaatgga aacacctaag aatgatcgta aaaacatgag aaaaggccct
    15721 aaaatcttta ataaatagac gtgctaaa
//
LOCUS       DACDHL010000055        14994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDHL010000055 DACDHL010000000
VERSION     DACDHL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.055"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..608
                     /locus_tag="IV228_001857"
     CDS             <1..608
                     /locus_tag="IV228_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717746.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="IV228_001858"
     CDS             628..1656
                     /locus_tag="IV228_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8717747.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="IV228_001859"
     CDS             1653..2411
                     /locus_tag="IV228_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8717748.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="IV228_001860"
     CDS             complement(2497..3237)
                     /locus_tag="IV228_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8717749.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="IV228_001861"
     CDS             3298..4551
                     /locus_tag="IV228_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8717750.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="IV228_001862"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="IV228_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="IV228_001863"
     CDS             5222..5548
                     /locus_tag="IV228_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8717751.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="IV228_001864"
     CDS             5616..6329
                     /locus_tag="IV228_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8717752.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /locus_tag="IV228_001865"
                     /pseudo
     CDS             6639..7491
                     /locus_tag="IV228_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7612..9477
                     /locus_tag="IV228_001866"
     CDS             7612..9477
                     /locus_tag="IV228_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8717753.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9525..10934
                     /locus_tag="IV228_001867"
     CDS             9525..10934
                     /locus_tag="IV228_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8717754.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11042..11743
                     /locus_tag="IV228_001868"
     CDS             11042..11743
                     /locus_tag="IV228_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8717755.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11902..12381
                     /locus_tag="IV228_001869"
                     /pseudo
     CDS             11902..12381
                     /locus_tag="IV228_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13229..13804
                     /locus_tag="IV228_001870"
     CDS             13229..13804
                     /locus_tag="IV228_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8717756.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13804..14244
                     /locus_tag="IV228_001871"
     CDS             13804..14244
                     /locus_tag="IV228_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8717757.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14260..14961
                     /locus_tag="IV228_001872"
     CDS             14260..14961
                     /locus_tag="IV228_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8717758.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5066 a         2485 c         3161 g         4282 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    13141 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    13201 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    13261 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    13321 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    13381 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    13441 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    13501 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    13561 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    13621 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    13681 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    13741 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    13801 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    13861 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    13921 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    13981 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    14041 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    14101 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    14161 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    14221 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    14281 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    14341 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    14401 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    14461 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    14521 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    14581 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    14641 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    14701 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    14761 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    14821 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    14881 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    14941 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa aatt
//
LOCUS       DACDHL010000056        14485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDHL010000056 DACDHL010000000
VERSION     DACDHL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.056"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..503
                     /locus_tag="IV228_001873"
     CDS             207..503
                     /locus_tag="IV228_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717759.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLIEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKTLEEYVRQNNRILEILIEQNRGSEL"
     gene            505..927
                     /locus_tag="IV228_001874"
     CDS             505..927
                     /locus_tag="IV228_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717760.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEMEKKVNANQAKLNSMSKEISTMTEIIAEFADIHLKDAGILIGTNSTVYQEAKERVE
                     GKIKTNQTKKYSPKIKKPINEFVHARVDPFDPTQFLKR"
     gene            946..2538
                     /locus_tag="IV228_001875"
     CDS             946..2538
                     /locus_tag="IV228_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717761.1"
                     /translation="MTQSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSISSDEV
                     KGSDLKEIKQIFQKGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYIEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLTEVYKTLPDKRSEWNYGNENRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKKEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2550..2885
                     /locus_tag="IV228_001876"
     CDS             2550..2885
                     /locus_tag="IV228_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717762.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            3032..3226
                     /locus_tag="IV228_001877"
     CDS             3032..3226
                     /locus_tag="IV228_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717763.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAVENYPFV"
     gene            3258..5621
                     /locus_tag="IV228_001878"
     CDS             3258..5621
                     /locus_tag="IV228_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717764.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYDKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQAELQAVIQAVANTIGIQIRDMTESEVYPRELGQARGAYVHNEFTGQEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGRTLDEKEAVERGKIMNDVSRVANEFIQVISMEINQQRERGLEIQPAEVKQD
                     HKNTVETKNVVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFVEDPEHNQVILKGKSYG
                     KEIQTALPSEEFALGNQFRFSLETVSEVAKRKNEIYYEQQMSQKERV"
     gene            5682..7139
                     /locus_tag="IV228_001879"
     CDS             5682..7139
                     /locus_tag="IV228_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8717765.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVVNKGRDYRLEDHDSFV
                     FDRKKQRFYWNSQGIGGDIIELTQLFFIDDSIQNPKQRFKAALDYILKDESKVERVEN
                     LHFETQEYKHHPMDYQPLTEKGRTYLKEQRKFPEWLIEYGDQQGLILEMKPRSERKNY
                     IVKDDRLDYAIVFPWKDRHTDQVLGATYQGTIIDYERFGDRGTYKHIDINPTPNHGFN
                     VLLGSPKNLKFFESPIDLLSYVALNRETVKDTWLISMDGLKDAVVGHYVREAVTELAP
                     KEEFPESIEICVDNDRAGHIFWDKVHRLGVSNPFTGKAVYFEPDLPNDWQVPRKYQTI
                     YEEVGKEMNVAPEAIMAIHKIENNLTENNQIVSFGEIHGVFAQKLAPKEKVQTIDVKE
                     KCIEAAQQLKSCEKSDGTYDFDRFYRGKGKINEEIQISLKAKHYFEGYKNHDHEFVSE
                     VKKDWNDQWKHEIQQQEIRKQKRAMLFQQSRQQER"
     gene            7153..9357
                     /gene="topB"
                     /locus_tag="IV228_001880"
     CDS             7153..9357
                     /gene="topB"
                     /locus_tag="IV228_001880"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8717766.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIHDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYRYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWSIIQQAGADQGKKFKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAQILAQNGEFYAKLDPNETQPTQEAYEAYLKEKGVRLGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKVSAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDDYKKFLKAEAIPTPTHTPNNRYVNNKKVQEHYAIIPTKTFMSEVDFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLVTAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTNFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTAK"
     gene            9603..10199
                     /locus_tag="IV228_001881"
     CDS             9603..10199
                     /locus_tag="IV228_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8717767.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            10212..11111
                     /locus_tag="IV228_001882"
     CDS             10212..11111
                     /locus_tag="IV228_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8717768.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            11114..11599
                     /gene="ssb"
                     /locus_tag="IV228_001883"
     CDS             11114..11599
                     /gene="ssb"
                     /locus_tag="IV228_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8717769.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRVHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            11965..12225
                     /locus_tag="IV228_001884"
     CDS             11965..12225
                     /locus_tag="IV228_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717770.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIDSMYVRNPDHFTRSMGI"
     gene            12614..12874
                     /locus_tag="IV228_001885"
     CDS             12614..12874
                     /locus_tag="IV228_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8717771.1"
                     /translation="MKKELTKGNKTFPKRRQNQRKKFDYWGVPFSELFPNYHAPHTVE
                     CDCGERAKCIKSYRLYQCPTCGKKYTLSYGDYVLIDEKGKKR"
     gene            12874..13125
                     /locus_tag="IV228_001886"
     CDS             12874..13125
                     /locus_tag="IV228_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717772.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLVPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            13141..13578
                     /locus_tag="IV228_001887"
     CDS             13141..13578
                     /locus_tag="IV228_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717773.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGAYYTMKKEKNENE"
     gene            13571..14086
                     /locus_tag="IV228_001888"
                     /pseudo
     CDS             13571..14086
                     /locus_tag="IV228_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5484 a         2341 c         3038 g         3622 t
ORIGIN      
        1 taaagagcat aaggaaacgt gggaacactt ttaaaaaaag tgttccaata ggctgtgcct
       61 attacgtttc tctaaattaa ttgtgggaac aaaataagta aaaataggat agcaaaagga
      121 tagtaaagga tagtaaaagg atagaagcag gatagcgaaa aggataggct ccttttcgct
      181 atccttttaa aacggaggaa attaatatgg aagtaagaat aagaaatgta gatgagaaaa
      241 tcgtaagaga aattgataga agatgtgacg aactaagtaa ccgtacggga aataaatgga
      301 gtcgtaatga ctatttaaaa ttactgatag aaaatgattt tgatcgtcct ttgatggaat
      361 ataaacaaga aaagtttgac cagttattag aaaagttttc agagattcaa agctacaaca
      421 caaagacatt agaagagtat gttaggcaaa ataatcgtat tttagaaatt ttgattgaac
      481 aaaatagagg aagtgaactt tgatatggca aaggttatag ataaacacat ttactttcac
      541 caaacacata tcgatgtatt ggatgaaatt atttctactc atgcagaggt taaaaactat
      601 agtgaagctg ttcgcttttt atgtatgaat tatgatccag aaatggagaa aaaagtaaat
      661 gcaaatcaag caaaactaaa tagtatgagc aaagaaatat ctactatgac tgaaattata
      721 gcagaatttg cagatattca tttgaaagat gcaggaatac taattggtac aaattcaact
      781 gtttatcaag aagcaaaaga acgtgtagaa gggaaaataa aaacaaatca aacgaagaaa
      841 tattctccta agatcaagaa accaatcaat gaatttgttc atgccagagt agatccattt
      901 gatccaacac agtttttaaa aagatagaaa tgaaggagaa taataatgac tcaatcacct
      961 gcagttattc tcacttgtca atttaccttg cccaataaaa aatctttttc aacgtacatt
     1021 gattatatga caagagaaaa agcattggaa gaaaaggaac gacgcacgcc cgacgaagaa
     1081 caggaactgc aacgaatcaa gaatgcctta gaggattttt atattcctga aggagaaact
     1141 ttttcggaga gtaagacgga taaagaattc agcgaaacgt cgaatgaagc tcaatcgttg
     1201 atgggaaatg gaatcaactt tgacaagtta gaggagcaag attttaccaa atatctttct
     1261 tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag tgaacaaaaa
     1321 gaataccaac gcttgaacca agcaataaaa aaatatcaaa cagaacccca taaaaaagct
     1381 tcaggaaatc ttcctggagt tttttctata tcttcagacg aagtaaaagg aagtgatctg
     1441 aaggagatca agcaaatctt ccaaaaaggg caacaaaaag gctcaatcat ttatcaagat
     1501 gttgtctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc caaaacagat
     1561 accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac attattcgag
     1621 aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa cactaagcat
     1681 atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta tattgagatt
     1741 gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac attagacgaa
     1801 atgaagacta attatgtaca tgagttagaa cgtttttctt atgaaaagaa tcgaatgatc
     1861 ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac cttaacaaaa
     1921 gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaac tgaagtatat
     1981 aaaaccttac ccgacaaacg ttcagaatgg aattatggaa atgaaaatcg aactaaactt
     2041 tcaccatata caagagaaaa gctcgatgaa ctaaccaaat atgttttaac aaaagaacct
     2101 gaatatcaag aatatataca gatttcaaaa gcgttaaaaa aagaagccaa aagtctttat
     2161 ggagaaagca aaagagagtc aaaggatgca gagaaaaacg ctctctttga tttgaaaaag
     2221 cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat aaaaccatta
     2281 atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg aacactcaaa
     2341 cagttgccaa caaatgaata caagtatagt taccaagaat tcgtgaagcg taggaaacaa
     2401 ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac aattaatgca
     2461 catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag aatccaaatg
     2521 gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa tggaaagtca
     2581 tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta gaaaaagagc
     2641 atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat caatttttag
     2701 ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaacggt gaaaaatatt
     2761 ttattattca agaatacgaa ggtaagttag caccttatta tactacttgg gaaataaatg
     2821 aagaggggat caataatttc ccatgtgaaa gcattgaaga gtctatttct ctcacggaat
     2881 gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact cttttttaat
     2941 gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat caaaagaaag
     3001 tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg gtgttgtggt
     3061 tgaaaaaagc agggacaatc tcgtttttgt gacagaaatt caaacaggta gagcttttgt
     3121 agtcacagac aaagctgcaa aagcgtatca aaacggcgat attttaacgt taaatctgac
     3181 tacgaagacg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa acgatatttt
     3241 acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt cgtttgggaa
     3301 agtccaaaag aaaattagta cggttgcaaa acgaattaaa tgatcaattt caaaagctat
     3361 ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat ggtcgttcat
     3421 ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa gaaattaagc
     3481 agcaagaaaa acaggtagag aaacttgaaa gacaagaaga gctaaaggaa atgggctata
     3541 acaagtacgg cggactggat atgacgatcg agaatattcc aagaatcaaa gaagaaatcg
     3601 aacgctttga aaaaggtgaa tcgacttttt cagccgctac gatccgaaaa taccaacgga
     3661 aattggaaac attagaacag ctgaaagaaa gatcagaaaa aggaaaagaa aaccttcttc
     3721 ctgaagtaca agcaatcatt gacagcggac gagtgacgca atggaaaaaa aatccgacca
     3781 tttattttct gaaaggctat cgaaaagtcg ctttagaact gaatgaaaac ggggaatttg
     3841 aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt cgtgaattac
     3901 ttgaagaggc tgcaccaact gattctctga aggagaaaga aaacgatcat ttctatcgag
     3961 tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg gagatcgtaa
     4021 cattagaatt gatcgatcaa ctcaaaacgt tggataacca atacgccgca gaagcaggtt
     4081 attataaatt ctattttgaa gaagtgaaga acggagaagt agtcgatcag atccgaatgg
     4141 atctagggga tggcgtcaac agtaatcaac ccatctattt agagttagca aaattgtgcg
     4201 aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt tcgaaagaac
     4261 aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga acggcagaag
     4321 agattaaaca agaagtcaaa tccctttcgc aacaggcact ggatgcggtg aaaaagcata
     4381 cccattctcc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt ccagaacgat
     4441 cctttagaaa tcaattattg atcgaggaac aatttccagg tgcaacggct tgtttaggtc
     4501 gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg agaacaaggt aacaagatct
     4561 ttgtcagaaa aacggtcaaa ggattctatg ataaaggacg gggctttgtt cgagaaacag
     4621 aggctacacc agaagataaa aaacgaatcg aaagtgggca aatcgaagtg atcaaaaaac
     4681 cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttgaaa cctgaagact
     4741 atccgaaaat attcccgaac cgtgtctttg attttcaatt ggataaaaaa ggacaagcag
     4801 aactacaagc tgtgattcaa gcggtagcca acactattgg gatacaaatt cgagatatga
     4861 cagagagtga agtctatcca agagaattag gacaagcacg tggggcgtat gttcataacg
     4921 aatttactgg ccaagaagaa attgtcatga atactagaaa tacaccaacg cagcatttag
     4981 ctacaagtat tcacgaatta gcgcacgcta gaatgcacaa attttcagaa ttagataccg
     5041 caaccaaaga actgcaggca gaaatgacct cttacattgt gtgcaaacat tttggaatgg
     5101 atacgtctga aaaagcgatt ccttatattg cgacatggac gaaaaatggt cggactttgg
     5161 atgaaaagga agctgtggaa cgtggcaaaa tcatgaatga tgttagtcga gtggctaacg
     5221 aatttattca agtcatttca atggaaatca atcaacaaag agaaagaggg ctagaaattc
     5281 aaccagcaga agtgaagcag gatcataaaa atacagtaga aacaaagaat gttgtgcctg
     5341 ttggttccct ttggatcgat aagaaagacg gaaaagtcat ggaagaagat acgggtcgaa
     5401 cgttacactt gaaggatgcg gcatttgttg aagatccgga gcataatcaa gtgattctaa
     5461 aaggaaaaag ttatgggaaa gagatccaaa cggctttacc ttcagaagaa tttgcgctag
     5521 gaaatcaatt ccgattttca ttagaaactg tatcagaagt ggccaaacga aaaaatgaaa
     5581 tctattatga acaacaaatg tcacaaaaag aacgagtata aaacaagcaa gaaagactat
     5641 ctgaaaaatg gatagtcttt tttcttttgg gaggtttgaa aatgacagag aaaaaaatga
     5701 gtcttattga tcgatgcaag caaatggata ttgtagattt tgctcgaaac aatggtttag
     5761 cggtagtcaa taaaggacga gactatcgtt tagaagacca tgattctttt gttttcgatc
     5821 gaaaaaaaca gcgattttat tggaacagtc aagggattgg tggagatatt attgaattga
     5881 ctcaattgtt ttttattgac gattctattc aaaatccaaa gcaacgtttt aaagcggcgc
     5941 tagattatat tttaaaggac gaaagcaagg tagaacgggt ggaaaatctt cattttgaaa
     6001 cacaagaata caagcaccat ccaatggatt atcaaccact cacagaaaaa ggacgaactt
     6061 acttaaaaga acaacgaaaa tttcctgaat ggttgattga atatggagat caacaagggt
     6121 tgatcttgga aatgaagcca cgaagtgaac gaaaaaacta tattgtgaaa gatgatcgtt
     6181 tagactacgc gattgttttt ccttggaaag accgacatac agatcaagta ttaggagcaa
     6241 cttatcaagg aacgattatt gattacgaac gttttggtga tcggggaacg tacaaacata
     6301 tcgatatcaa tcctactcct aatcacggat ttaatgttct tcttggttct cctaaaaatt
     6361 taaaattttt tgagagtcct attgatttat tgagttacgt tgctttaaat cgtgagacag
     6421 tcaaagatac atggctcatt tctatggacg gactgaagga tgcagtagta ggacactatg
     6481 ttcgagaagc ggtaacggaa ttagcgccga aagaggaatt tccagaatcg atcgagattt
     6541 gtgtggataa tgatcgagct ggacacatat tttgggataa ggtacaccga ctgggagtgt
     6601 cgaatccttt tacaggaaaa gcggtgtatt ttgaacctga tcttcccaat gattggcaag
     6661 taccgagaaa ataccaaact atctacgaag aagtggggaa agaaatgaat gttgcaccag
     6721 aagcgattat ggctattcat aaaatcgaaa acaatctaac ggagaataac cagattgtat
     6781 cattcggtga aattcatgga gtatttgccc aaaagcttgc gccaaaagag aaagttcaga
     6841 ccattgatgt gaaagagaaa tgtatagaag ccgctcagca gttaaaatcg tgtgaaaaat
     6901 ctgatggaac gtacgatttt gatcgctttt atcgtggaaa agggaaaatt aatgaagaaa
     6961 ttcaaatttc attgaaggcc aaacattatt ttgaaggtta taaaaatcat gatcatgaat
     7021 ttgtttctga agtgaagaag gattggaatg atcaatggaa acatgagatc cagcaacaag
     7081 aaatcagaaa gcaaaaacgt gctatgttat ttcaacaaag cagacaacaa gaaaggtagt
     7141 gaaggaagca ctatgaaaac tgtcatttta gccgaaaagc cgtcacaagc gaaggcttat
     7201 gccgatagct tctctaaagc cacccgaaaa gacgggtatt ttgagattca tgatcgattg
     7261 tttcctggtg aaactgtgat cacctatggt tttggtcatt tggtcgagtt agattcgcca
     7321 gatatgtacg atgaaaattg gaaacaatgg tcattagaac atttgcctat ttttcctaca
     7381 cattatcgct atcatgttcc taaagataag aaaaagcaat ttaacgtagt gaagcagcaa
     7441 cttcaatcgg ctgataccat tattattgcg acagatagcg atcgtgaagg agaattgatt
     7501 gcttggtcga tcattcaaca agcaggcgca gatcagggaa aaaaatttaa aagactttgg
     7561 atcaattcat tagaaaaaga agcgatttat caaggatttc agcagttacg agacgcagga
     7621 gaaacctatc cgaaattcga agaagctcaa gcacgtcaaa tcgctgattg gttgatcggc
     7681 atgaacggta gtccactata tagtttactc ttgcaacaaa aaggcatacc aggaagtttc
     7741 tccttaggca gagttcagac cccaacgctt tatatgatct atcaattaca agaaaagatc
     7801 aggaacttca agaaagaacc ctactttgaa ggtaaagcac agatactcgc tcaaaacgga
     7861 gagttttatg ccaagcttga tccgaatgaa actcaaccca cacaagaagc atatgaagca
     7921 tatttaaaag aaaagggtgt tagattagga aaacagccag gtacgattct tgaagtcgaa
     7981 acagagaaaa aaagcgcggc aagtccgcgt cttttttctc tatcaagcct tcagtctaag
     8041 atgaatcaac tcatgaaagt tagtgccaaa gacactttgg aagccatgca agggttgtat
     8101 gaaggaaagt atctaagcta tccacgaacg gatacccctt atattacaga aggggaatat
     8161 gcgtatttgt tggatcattt agacgattac aaaaaatttt taaaggctga agcaattcct
     8221 actcctactc atacaccgaa taatcggtat gtgaataata agaaagtcca ggaacattac
     8281 gcgatcattc ctacaaaaac attcatgtca gaagtggatt ttgaacaact aagtccgctg
     8341 cagcaggcta tttatgaaca agtgttaaaa acaacggtgg ccatgtttgc ggaaaagtat
     8401 acctatgaag aaacaacgat cttgacgcaa gttcagcagc ttcagttaaa ggcaatcgga
     8461 aaggtgccgc tagatctagg ttggaagaag ttatttggaa aagagagcga cggaaaagag
     8521 aaggaagaag agccgctact tcctaaagtg acaaaaggag aaatggtgac ggtggatcta
     8581 caagttcttg aaaaagaaac caagccacca caaccttata ccgaaggtac gttggtcact
     8641 gcaatgaaaa cagctgggaa aaccgtggat agtgaagaag cgcaatccat attgaaagaa
     8701 gtggaaggaa tcggtacaga agcgacacgg gcgaatatta ttgaaacctt gaaacaaaag
     8761 gaatatatca aggtagagaa aaataagctc gtggtgacga ataaagggat tttgctttgc
     8821 caagccgtgg aaaaagagcc gctgcttacg agcgccgaaa tgaccgccaa atgggaaagc
     8881 tacttgttaa agatcggtga acgaaaagga acacaaacca cttttctagc caatatccaa
     8941 aaatttgtct ctcacttatt ggaagtcgta ccagggcaaa tccaatccac taattttgga
     9001 tcgacgttac aagaagtgaa agcagccagt gaaaagcaag aagccgcgcg tcatttagga
     9061 atttgtccaa aatgccagga acaagaagtc ttactctatc aaaaggtggc agcttgtacg
     9121 tctgaagcct gtgattttaa attatggaca acgattgcca aaaagaaact cacagcgaca
     9181 cagctgaagg agatcattca aaatagccga acaagccaac cagtgaaagg gttaaaaggt
     9241 cagaagggtt catttgaagc aacgattgtt ttaaaagaag acttcactac aggctttgaa
     9301 ttttctgaaa agaaaaaaac gaacttcaag aaaagaacac gccgcactgc aaaataatgg
     9361 agggttaatc ttatgcgtaa taccacacac aaacaacaag aaatcgaaaa tcaaaaagcc
     9421 aaaattcgtt cagaacagga aaaaatgcgt gccagcaaac gaagactgca gcgattaaag
     9481 aaccagcaac ggattcaaaa agaaatcgaa ttagatcgat aaaatcttcc tgattgttgt
     9541 atacgtataa acttatgtta tactatattt ataagtttat acgtatataa aggagggaga
     9601 aaatgacaaa agcaatggta tataagctac tagaagaatt taatggaact ttggctttga
     9661 aggatgctaa aaaagcgggc atttctcctg ttacgatcaa acgtatggta gatcgagggg
     9721 agctggatcg agagtatcca ggctttttca ccttacctgg tcaatttcct gatgaattgt
     9781 ttatggcgca aaaaaaatat gaacgcggca ttatttcgca tatcaccgcg ttggatttat
     9841 atgatttaac ggatatgatc cctagacaga tcgatctaac cgtaccttac ggttatcacg
     9901 tttctgaaaa gggattgaag gaattcgcgg ttgagttaca ctattcaaaa gcggaatggt
     9961 atgaattagg caagatcgaa ataaagagtc gctatgggaa tcctgttata gcttatgatc
    10021 cagaaagaac cttgtgtgac atttggaatc cctggtataa cgttgaggat gaaatcaaag
    10081 taaaagcaat caaaaactac atggagtcgg acagaaaaaa tcttagaaaa ttgaatgagt
    10141 atcgacgaat tttgcctaca gataaaacga tgcgctctta tattatggct ttaaattaaa
    10201 ggagaagaca catgacacct acgcaattaa aagcaaaagt gaagaatatt tctagagaaa
    10261 aagaagttga tccgcagctt gttttaagac attttatgat ggaaaaattc ttggaaaaaa
    10321 tctcagagtc tccttatcgt gaaaattttg tgttaaaagg cggcttctta attggctcaa
    10381 agtatggcat tgaaaatcgt acaaccaaag acattgatac gactttacgg gaaatgaaag
    10441 taaccaaaga aacattaaca acggttctaa acgatatttt ttcaacgccg acgaaagaag
    10501 gcattcaatt tgagattcaa ggaatgaaag aaacgagaga agccgattac tatccaggat
    10561 ttagcttacg tgtgctggca catttggaaa atatgcggcc agattttaag gtagatgtga
    10621 ctacaggtga ctcgatctat cctgcaacga tcactcacag tcataaattg atgtttgaag
    10681 atcgaacgat tcctttggaa tcttatccta cagaacaaat tattgccgaa aaattaagtg
    10741 caacctttga ttttttaaca gataattcga gaggaaaaga tttttatgat ctctatacta
    10801 ttcctaaaat ggaaaagatc gatgagaaga ccttgaaaga tagtgtgaga aatacattta
    10861 cacgtcgagg aaaaactgat ccgttaaaga tttattacga aaaggatatg gatgtactga
    10921 aaaatagtca tgagctaaaa gatacctgga agaaatatca aaaaagcaat ccttatgctg
    10981 cgtcgatcac gtacgaagag acaatggatt cgattagtaa tttgatggaa aaagtgattc
    11041 acgtcgaaga aaaagagcga aaaatagctt atcaaagaca ccaacagatg aacagagaaa
    11101 tggagagata aaaatgatca ataatgtaac tttagtagga cggttaacca aagatccaga
    11161 tttacgttat accgcaagtg gcacagcagt ggctactttt accctagccg ttaatcgtaa
    11221 tttcacgaat caaaacggca atcgagaagc tgattttatc aattgcgtga tttggcgcaa
    11281 accagcagaa acgatggcga cattagctaa aaaaggcagc ttgatcggcg ttgtcggacg
    11341 gattcaaaca cggacttatg acaaccagca aggacaacgt gtgtatgtga cagaagttgt
    11401 agctgataac ttccaactat tagaaagcaa agcagcgaca gagagccgtg tgcacgctga
    11461 tcaatcgtcc acgagtccaa gcaccaccac ttttgaacaa cgggatacag cgacgcctaa
    11521 caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg atattagcga
    11581 tgatgatcta ccgttttaag tagcattttc tattggttgc atttgctacg gtttatgtta
    11641 taatttagtc aacaaaaaga attttcaatc tataaccaaa aaccactagc tggcgggcta
    11701 gtggttttct tttacgtttc tattataaga attaacgggt aggcttactg tctttcttgg
    11761 caaatttaca atcaagccat ttgcagatat agtgcgcggc tacttctgcc atgattgcaa
    11821 aaacaaaatc aaaaagaatt ttcaaaccta taacctcctc cccttgccag attaggccgg
    11881 ggtagcagac agcaagacta ttatagcata atcgaaatat ccaaatgatt ggatttttgc
    11941 tataatttta gcgaggtgat tgtgatgaac aaatatgttg gtttgcttga taaaatccga
    12001 gtaataaaaa cgcaaccgtt attggttcgc tttactttac aaacgattca tgaatcgatt
    12061 aattgtgtag ttgcagatat tgaaattatc gataaattgc taattatgga tgatggaaaa
    12121 tacaatattg ccgtcacagg ccacttcaat aaacgaaatc agctagttat tgatagcatg
    12181 tatgttagaa atcccgatca ttttacgcga tcgatgggca tataaaaaaa gaagagttgc
    12241 aatcctaatg taggactgca gctctttttg ttttacatag aaacaggaag aaagggaaag
    12301 gcctgtttag gaaggcacaa gcgccttctc aattgaaaaa cctttctctc actccaaccg
    12361 ccaccgccgt tcgagaagaa aggaagtgaa cgttgcacgt tcactaagat aaggggtctt
    12421 gctttgcaag acaaaatagg aaaagcttcc tctcaccaaa aggttcgttc ctgttcgcta
    12481 tctctttatt gatttgccag accggcaaca agttgccttt tatcaccaaa aagcgacaag
    12541 gacaagggta aatggcacgc tgcgcgccct tgtccttgtc gcttttcgtt gattcattct
    12601 ggccaacaaa tcaataaaga aagaactaac gaaaggaaat aaaacctttc ctaagaggag
    12661 gcagaatcag cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
    12721 ctatcatgca ccacatactg tcgaatgcga ttgtggagaa agagcgaaat gtatcaaaag
    12781 ttatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt atggcgatta
    12841 tgttttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa attattgact
    12901 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
    12961 gctttacttg ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
    13021 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
    13081 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
    13141 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
    13201 atggaggata tgacgttcct ataccatgaa acacgtgtac ccaaaaaaca ttacaaggaa
    13261 aaattgtcag tcgcagtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    13321 acgtactatc gaacattgga agaactgaaa aaacaaaatg gaaaatggtt tttccaagcc
    13381 ttactatgct tggaagcagg cgtcaaacca agcacaatca aaccttctga ataccaagct
    13441 ttagaactaa cgtatggaaa atttatcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    13501 tggttggact actttgaaaa tgtcaacaag tatggcgcct attacaccat gaaaaaggag
    13561 aaaaacgaaa atgagtaatg tggtttatca actaacagtg gaagatgttt ctcacttttt
    13621 aacaaacaaa gaacatttga gtgaagtagt cagacggatt caaaaagact ttagttgtat
    13681 ggaagaaatg aagtacttta ttcaaagagt ggaagaacag ttgtatatgg aaagcgaaac
    13741 gagtcaaacg aaggtcacag tcagccataa tcatttaaac gtgtataaac gtcgtcctga
    13801 aacggtaaaa aaaggaacag agcttctacg actagaaaca gaaaaataca tcatgaatgt
    13861 gtacctagaa aggagtgagc tggtcgaaaa ctttgtcagc tacacgattt ataggaaaca
    13921 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    13981 aatggaagag ctaaacagca aaaatgcgac ggtagacgat tattaccttg tatttactag
    14041 atttttgacg tgtgggacgg aatctatcgt gttaatggga atggaaataa gtgggcaatg
    14101 cccgataacc taagagaaag aaggagattc atatgaataa aatcattcct ctattgttag
    14161 tactatttgt cggagaaaca cttatgcgtc atagcgctgt tgttcatgga aatttcttat
    14221 tatttgtagg gatattagtg ttttgtttga tcttttggcg atttgtatta gccctcgtga
    14281 gtctaatatt caaattgatt gcagccatat ttttcatagg gttatttcga tgaaaggaga
    14341 gagtaccaat gccaattgca ataggaaata aacggttacc cgtgacatta gatgaaaaga
    14401 gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct aagattatgt
    14461 gtattgcgtt agatctattg attgc
//
LOCUS       DACDHL010000057        14483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDHL010000057 DACDHL010000000
VERSION     DACDHL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.057"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            279..1127
                     /locus_tag="IV228_001889"
     CDS             279..1127
                     /locus_tag="IV228_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717774.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="IV228_001890"
     CDS             complement(1298..1639)
                     /locus_tag="IV228_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP8717775.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="IV228_001891"
     CDS             1725..2432
                     /locus_tag="IV228_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8717776.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="IV228_001892"
     CDS             2419..2742
                     /locus_tag="IV228_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8717777.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="IV228_001893"
     CDS             complement(2831..3868)
                     /locus_tag="IV228_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8717778.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="IV228_001894"
     CDS             complement(4315..5298)
                     /locus_tag="IV228_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8717779.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="IV228_001895"
     CDS             complement(5714..6484)
                     /locus_tag="IV228_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717780.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="IV228_001896"
     CDS             complement(6477..7412)
                     /locus_tag="IV228_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8717781.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="IV228_001897"
     CDS             complement(8006..9655)
                     /locus_tag="IV228_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8717782.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="IV228_001898"
     CDS             complement(9850..10746)
                     /locus_tag="IV228_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8717783.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="IV228_001899"
     CDS             complement(10739..11419)
                     /locus_tag="IV228_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8717784.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="IV228_001900"
     CDS             complement(11420..12202)
                     /locus_tag="IV228_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8717785.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="IV228_001901"
     CDS             complement(12195..13112)
                     /locus_tag="IV228_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8717786.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="IV228_001902"
     CDS             complement(13115..13399)
                     /locus_tag="IV228_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8717787.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(14100..>14483)
                     /locus_tag="IV228_001903"
     CDS             complement(14100..>14483)
                     /locus_tag="IV228_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717788.1"
                     /translation="KAFISNGESLKNVTARIRKENEVDIVFGIDHECNSVIYVTQSAN
                     LILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFVSEQKGSFSKKIGIEVIESIMC
                     HNHITLEELEILIGQKNNNTKIQVS"
BASE COUNT         4309 a         3087 c         2500 g         4587 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggttaa acaaaactct
    13921 attttattgc attttacagg aatgtatttc atgcgaaatt aataagtctt tgtttataga
    13981 gttttactag ggtataaaaa gttgtatgtt atcattacct caaaacatta cctcagcttg
    14041 gcgagttggt tgattgaaga ataaatcatt tatctagata tctcaataaa aaatgatttc
    14101 taagaaactt gtattttcgt gttattattt ttttgcccta tcaaaatttc taattcttct
    14161 agcgtaatat gattatgaca cataatcgat tcaattactt caatacctat ttttttcgag
    14221 aaagatccct tttgttcact cacaaaaata ctttcagaag acatatataa ggaacgaagt
    14281 tttggaatta gtatatcgta caaatagacc accataaaat cgtccttgca accattctca
    14341 aaaagaatta aatttgctga ttgtgttaca tatatcactg aattacattc atggtcaatt
    14401 ccaaaaacaa tgtctacttc attttctttt ctaattcgcg cagttacatt tttcaaactt
    14461 tcaccattac ttataaatgc ttt
//
LOCUS       DACDHL010000058        14130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDHL010000058 DACDHL010000000
VERSION     DACDHL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.058"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..918
                     /locus_tag="IV228_001904"
     CDS             <1..918
                     /locus_tag="IV228_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8717789.1"
                     /translation="ALDDNTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLS
                     TYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIG
                     FLSNSPIGKKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISN
                     ALRTAYIATQHIDNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIE
                     DFIRPVFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKL
                     KMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            1030..2481
                     /locus_tag="IV228_001905"
     CDS             1030..2481
                     /locus_tag="IV228_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8717790.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2830..3501
                     /locus_tag="IV228_001906"
     CDS             2830..3501
                     /locus_tag="IV228_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8717791.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            4049..5488
                     /locus_tag="IV228_001907"
                     /pseudo
     CDS             4049..5488
                     /locus_tag="IV228_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5833..6000
                     /locus_tag="IV228_001908"
     CDS             5833..6000
                     /locus_tag="IV228_001908"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8717792.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            6259..6474
                     /locus_tag="IV228_001909"
     CDS             6259..6474
                     /locus_tag="IV228_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717793.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6662..6949)
                     /locus_tag="IV228_001910"
                     /pseudo
     CDS             complement(6662..6949)
                     /locus_tag="IV228_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(7136..8047)
                     /locus_tag="IV228_001911"
     CDS             complement(7136..8047)
                     /locus_tag="IV228_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8717794.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            complement(8066..9070)
                     /locus_tag="IV228_001912"
     CDS             complement(8066..9070)
                     /locus_tag="IV228_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8717795.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(9067..11175)
                     /locus_tag="IV228_001913"
     CDS             complement(9067..11175)
                     /locus_tag="IV228_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8717796.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            complement(11180..13627)
                     /locus_tag="IV228_001914"
     CDS             complement(11180..13627)
                     /locus_tag="IV228_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8717797.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13614..14003)
                     /locus_tag="IV228_001915"
     CDS             complement(13614..14003)
                     /locus_tag="IV228_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8717798.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
BASE COUNT         4434 a         2632 c         2581 g         4483 t
ORIGIN      
        1 gctttagatg ataatactat taaaaaagta ggaattattg gagcaggata tattggagtt
       61 gaaattgctg aagccataag aaaaagaggg aaagaagtat atcttttcga tgttgcagat
      121 agagtgttgt cgacttatta cgaccgtagt ttttcagata aagttgaaga aatactatca
      181 aaaaatggaa tacatctttg tctatcagaa agtcctaaaa aatatttagg aagaaaaaag
      241 attgaaaaaa tagtcactac aagtggtacc caatactcta tggatatggt cgtacaagct
      301 ataggatttc tatctaatag tccaattggc aaaaaggaac tcttgagaga tgttagtgga
      361 gcatatctaa ctaatgaata ccagcaaaca aatataaaag atatatatgc tattggggat
      421 tgtgctacaa ctttttttac gtccattaat agaagtagtg ttgatttttc tatttctaat
      481 gctctaagaa cagcttatat tgcaactcaa catattgaca atcaagattt tacaccttct
      541 ggttcccaat ttacaaatgg cttttctata tttaattaca atttttacgc agcaggtttg
      601 aatttaaaaa cagcacgatt attaagtgag tctatagatt atatagaaat agaagatttc
      661 attagacctg tttttttgag aaaaaatagt cgtgtaaaat taagaattag ctttgaaaaa
      721 aatacaaaga gaatagtagg tgtacaactt tgtagtcaag aagatttatc tggactatta
      781 tcaatgtttt ctcttgctat agaagaacaa attacattag acaaattgaa aatgttagat
      841 tatttatttc atcctagttt tagccaaccc tataatttta ttttaaaggc agtaacagga
      901 ttaaacattg aaacataatc gttactaaat gaaatcaaga aatatactgt attactcaat
      961 tcattgaaaa tatttatcat aatttttaat agaaaacact attaaagaga aaaaagagag
     1021 gtaaatcaaa tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc
     1081 tttatagcaa cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa
     1141 gaaagaaccg agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt
     1201 ttgacaactt tatcaagtga aagtagtacg acttcaaatg ctgagtcttc aacaacttca
     1261 tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa caacttcgtc aagtgaaagt
     1321 agtacgactt caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca
     1381 agtgccgaat cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca
     1441 agaccaaacc atatagagtc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa
     1501 aatttttcga caccacagca aatagaatca acagaatctt tccaatcaca accaatacta
     1561 tctggtaatg aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa
     1621 attggtgagt ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt
     1681 gcacaagcga tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac
     1741 aatttatttg ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa
     1801 gatttgggta atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat
     1861 gaggaaagtt ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat
     1921 ttttatgcag gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt
     1981 acaggacggt atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc
     2041 tatgatttaa ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac
     2101 caagtgccta ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt
     2161 caccgaggac ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt
     2221 aaggttgtta aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa
     2281 gatggatcaa ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat
     2341 gttttgcaag gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac
     2401 cttcatttgg aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa
     2461 accttattat tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt
     2521 ggattcttag acctttgatc aggatattga gactggaatt gatatctcga ttacgatgag
     2581 tttgacaaat aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct
     2641 catattttga gcaggtttta ctagatgaaa acaatttatc tatcttgagg atttcgtgta
     2701 cgtatcgttc agtcttgtat tataaattag tcataaaatc agactaagaa aaattgaaaa
     2761 tatttttttc tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg
     2821 gtaggtgcag tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat
     2881 ggatataaaa aatatcgtta cctatgtgac tgtgggaatg aaacgattgt atatcgatca
     2941 aacttactat caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa
     3001 tataaaaatt tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg
     3061 cgaagtgaag gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta
     3121 tcggcaaagc gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag
     3181 catcacattg aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac
     3241 tgttcatgga aaacaaaatg ggtttgtcaa tgtgagtgtg gcagtatgtg tgagataagt
     3301 tatagtaact tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca
     3361 aggacatgtg ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat
     3421 acaagtggtg ttaagggagt ttatcgttct aaagagaaat ggattgcgta tataacttta
     3481 aaaaaaagag gtactattta ggatcgtatg attctattaa gagtgcagct caagttagaa
     3541 aagatgctga aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat
     3601 tattgtggat aataattaac agtgatatat aaacatctat aatatattat tcatactatt
     3661 gacatttagt ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg
     3721 gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat
     3781 tttcttacgt ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt
     3841 tagaaattgc tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa
     3901 taaagcatct caaacttaca tggttacatg agaataacaa atcgaaatta ctcaagcttc
     3961 ctatggctag agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct
     4021 acacgatcat tagaaaggaa atcctattat gcatagttat caagaagatg caaaagtcca
     4081 aaaaaatcgc tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga
     4141 tggcagtatt gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa
     4201 tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt
     4261 tggtaaaatt ggcgatagct gtgggaaaat caaagtattt cggattggta cgctgatctt
     4321 tgtcttgggc tcgctgttgt gtgggttcaa tcattcgcta ggtatttaat tgttcgcacg
     4381 ggtggtacaa gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga
     4441 agtctttccc atacatgaac gtggtcgggc attgggagca atcggtgcgc ttgtttcatt
     4501 aggctcgatt gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta
     4561 cattttttgg atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt
     4621 accaaaagat atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc
     4681 ggctacgatt atcagccttt ttggaacaat ctttttgggg caagaaagtg gctttttcca
     4741 tccagtcccc cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt
     4801 tgaaaagaaa gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac
     4861 catgagcttg attacagcct tgttgatttt ttcttccaac ttctttgtta atgtagtgat
     4921 tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc tatgccggac tgttgatgat
     4981 ggtctttccc ttgatgatgg tcgtcggggc gcctttaagc ggatacctga ccgaccgggt
     5041 tggcccggag atttttgtat tagtaggctt gggtttattg tcggtaaccc aagtcatgta
     5101 tatgtttttg tcagtggact ctcctttgtg gctgtacatt gtcgctactg ccatcatggg
     5161 tgccggcaat gccttgttcc agtccccaaa taatacgatg gttatgagtt ctgtcactat
     5221 cgaaaatttg ggactggcag gctccatgaa ttcctttgcc cgaaatttgg ggatggtcat
     5281 cgggatttcg ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccaacg
     5341 agtaactaac tatatcgcag atcggccgga tgtcttttta tatggaatga agattacctt
     5401 tttaggcagc tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa
     5461 acaaggaggt attcagaaaa atgtctgaaa aaccattgtt gaagaatttt tggaggcctg
     5521 cgtatgggtg agtgggattt ccatggatga tggaagatga agtgaacttc tgaaaaccga
     5581 aaaactcaag tattatcttc aaaatactag tactcaatta gatgctcgat ttgattacaa
     5641 gaagactgcg aaagccactg gcaaccgcaa gctaggtatc caccgatgga ctacgtttta
     5701 gaggcaactg gttatgaata cggagctaac gctctcatct gcataagaat gcaacaccca
     5761 gattatgtaa agattttcga tacttctgtc aaaaaaatca tcaagataga taaatgattt
     5821 ccgtctagtc aaattggttc agaatgtgga cacacagatg gtaagaagtc attcacaatt
     5881 cgagaatgga cttgtcctat ttgccgtaca catcatgatc gaaacgtcaa cgttagtatc
     5941 aatattttga tcaagggcta tgtatacaag cattgcctta gaaaacatca agaaccttag
     6001 gaacaacggg gatagattgg ttaataagag atatcgctgt tagtaaagac ataggcttgc
     6061 aagtatactc ttttatcaag aattccgtgg ctttagtcat gataggttta atactatgaa
     6121 aattttcttt agcatttttg atttaggttc cttttctaat tataataaaa tgaccactac
     6181 agtaacctac tgaaatatat aaacagccac tgtagtacca ttttcaaagt agtctatgag
     6241 aggagaatct tcattcttat gaacgctgga attgcggata tgaatttaat aaaaaaaacc
     6301 cttaatgatt ttacttccaa ttcaatatca aaaggtactg gtatcaattt aagcacaata
     6361 aaaaagctga aaagtggtga aagatcagtg gaaaagctga atcttttaga tgctataaaa
     6421 ataacggaat ttgctatgaa aaatggaaaa gctgagattg aaatatggag ataattatcg
     6481 tctagtgaac tacttgccac ttagctgaat ttaacgatcc ttagtgcttg aagtgggaga
     6541 ttcttgggaa tagagcatac ttgcaagctt atgtctttat taacagcgat atctcttatt
     6601 aaccaagcta tccccgtagt tctaaggttt ttacttttat ctaagctaat gatcgtattc
     6661 aaaggaaatt tttgaccctt agaaagtctc attttatcag acatgagctt aggcattgct
     6721 gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt agtctttatc
     6781 acgatttcac tcaactaagg taatgtgatg tgattctgat attctttcaa agatagtttt
     6841 ttgcataagc taacattaca tcatcaatga gatgtttaca atgtttcact actaaaataa
     6901 ggtggtaaca aagaagaaat aatgaatgat tatttgtatc taattccatg gtatatcaac
     6961 catttcttat atagactgat tatgacatgg attaaaataa aaaggcacta tgtcttaatt
     7021 gtcggttttc gagttaccga acggcattca tcacccacct atagaggatg ggcgacttct
     7081 gcctgaaata agttaaacta cttataactt ctcataaatt tagattgagt cattattatt
     7141 taacatcaat gattttccag tttccatttt tttgtaatat taatttgtac tgaaagtagc
     7201 tagtggtttt tgatacatta tcaaggtatt ttatctttgt actcactaga attttttttc
     7261 cgttttgtag gtaaactgga tcctctaact tcgaaaactt taagtcaata ttgattggtc
     7321 gcatcgcatc attttcaaca taatactcca attctttttc agaagctgat ggatataatt
     7381 taaagaacgt ttccaaaaac tcagcgattt cttctgtagt tttggcatca attttcgaat
     7441 cattttctac ttgttttgtc ttgtattttg ccctatcagg ttttgcaatc atagttggca
     7501 aactcgttac tatataattc ctgttttcat cttcaaagat cgtcactcgg tatgcagaag
     7561 aaatcgattg tgcctttttt ccctctgtaa tcttttgctc cactaaaaat gaaacgacaa
     7621 attcctccga gctcttttca acatctaaga cttttactga ctgaacttcg gaattagtgg
     7681 gaatatctgc tcgaaccata tcttgactta aagcgaaacc ttcttctgta aggtactgtt
     7741 cgaggttgtt cattctcttt tcaattgctt ctttgttatt tttccacgaa aaatattctt
     7801 tcgaaaaatc cttcgtaaag ttttcaatac cagaagtatt cactactttc tctttaataa
     7861 ccactttttc atgagttgta tgttgatcaa tggcggtgaa atttttataa acgccaaaag
     7921 taagactaca agataataaa agccaaaggg caagcaccat ttttcgatgc ttacccacgg
     7981 atacgactcg ttcttttttc ttttttaggc tcttttcctt ttgattccgt tcaattttta
     8041 tcttcattct ttttcctctt ttctcttatt tgtaccgtcc agcaccgata agatgctgct
     8101 gccaatagct ttctgttaga ttcgtccagc ctactggatc tcctgcatga aacatgcgat
     8161 tctcccctgc ataaataccg acatgggtca cataagtacc ggcatcataa gtcgaatgaa
     8221 agaaaaccaa atcccctggt ttggcatctt ttaggtcaat atgggtcatg gcatcatact
     8281 gatcttgagc cacccgagga agtttcagac caactttggc ataacaccat tgagtcaagc
     8341 cactacaatc aaatcctgtt tcgggtcttg acccaccaaa tacataagcc gttccttcat
     8401 acttaaacgc ctcgtcaaaa atcccttgga ctgttttatc gtcaaactgt gttgtagtta
     8461 ggtattgctt caccaataag caataaaaca tattgccata gttatagcgc catccaccat
     8521 tttccttatt cgcgatggga ttagggtaat cgattttttg accgcaagat ttttcctttg
     8581 aaaaagattc agctaaagca aaactatgct tcttcccgtt ttttgccaca tagtccaaat
     8641 aaccaccgcc aaagttatag gcctgaatga tactatcttg atcacaacct aattctttgg
     8701 aacgcttaac caaagacgca aaatagagac atccttgttt gatggatgcc tcggtactta
     8761 atgaattcgg tggtaaaccg gctgactcag aactttgcat cacatcttcc attgtgccac
     8821 cagattccac ttgaataatc gctaaaagaa tcggaacata ctctgttata tccgattctt
     8881 tggcatattt ttccaccatt gcacggtgtt tcaaaacctc ttctgagaca ctactccccc
     8941 caggaggagc acctgaacta ctacttcctt cactgtcttc accaaacaat aagcccacac
     9001 acatcaagat acccataaaa gagattgtta ggaaagttaa cgaaatggca cttaatttct
     9061 ttacgttcat tttttctcac ctgaccttgg tttgggatga gggttcaaac ttttttgtac
     9121 ttttcgattg gttcgtttgg acagagtaga ccgccgtttc acttgttgaa aagaattttt
     9181 cttacttgag gattggttta ttccttttga aatgatttct tggctagcga attttcgttt
     9241 gatttcggga ttcgaatttg ctctcacaat ccgctttggt gctctatcct tttgagattg
     9301 gacctttgct tttatcggaa cattttccct tcttttccca tgaggattcc ctatttcgtt
     9361 caatactttg cgcttttcag ctattttttc gtctctacgt tgttgttgtt tttctcgcaa
     9421 ctctcctcga tttgcttttt cttggaccag tcctcgctta aactcttccg gtgctttttt
     9481 cgttttttca attcctttat ggaagttgta tttcaagttg gttggcgtat tccgtactcc
     9541 atcctttact tgtttggtct tatctttcac caaagcttgg gtatccagaa ccttgccgac
     9601 ttttctcccg gccaaattca ttcgggattg tttcttatta tttggaattg gcttttcctt
     9661 attcaagtca gatggtatct cgtgatcgtt cggtaatcgt gaattctttc gtagagaaga
     9721 accggatggc ttaaatttgg attttgattg ttcctttgcc aacttatttc caactgctgc
     9781 accaccacca aaagccaaag tccgtcccac atttcgattg agttgccgca atgttcgatt
     9841 cattaacatt tgcggttttc gcatgacccg tcttcccatt gattggctat cattactctg
     9901 gagattgaac atattcataa tgtcgcccaa tttgaaatag ataccggcaa aggtcacaat
     9961 ttgcagaaaa gcaaccatga aaaatggata ggttgccgaa atattgaaaa acatcgtgga
    10021 gatgctaaat gcagtcgtaa tcaccaaagt aactcctgct cgcatcataa tcgtattgaa
    10081 caagcgaata atcgccttct tgcccaaact ctcataagtc ggcaacatgg acaataagaa
    10141 actaatcggc aaaaacatcg caaagataat aaagagaatt tgcgagaaca acataatccc
    10201 tgttaggaga aagacaaaga aggatatgcc aatattgaac aacaccaaaa agaccacggt
    10261 ccctaagcgg ttcatggtct tggtaatact gagattcgca ttgtcattat cttcaatctc
    10321 cgctttgacc gcttcttccc gatccttgcc tttattttca tctggactta cagacaggat
    10381 tttgttaaca cgatcttcgc caacttcttc tatattggaa tcatcaaatt gcaagagtaa
    10441 ccacggttgt tggacttgaa tggaaaacaa gctatctcgg attaaatcca cactgtcctt
    10501 tccttggctt tctgaattcg gcacgacaat ctcggttcca aggctaagag ccgcttcgct
    10561 gacatctgag ctaaaatcat tgatttttgt gatataggtg ggcgcatacg caatgaacga
    10621 acccgaaagt agaaaaatca tcacaaaatt aatgacagcc cggactgctt tggtagtttc
    10681 tcgttttaat aatcctgtat acgccacata aactcctaga gctaaaatca tcaataatag
    10741 aaacccaaag taaaagcccg aggtctggag tccattttcg gtaatgccgg ctaaggtttg
    10801 gatattcttc ccaatacttt cagccgtatc tgaaatgaaa tccagcttat aggcttcttg
    10861 aaccacgtag cccgtggcat ttgataagta gagactaact gtccaaataa aattagtaat
    10921 ggcataaagt ccatacatta cgctcttccc taggccatca ccccaattcc aaggcagcca
    10981 atcccacgaa ctatccacaa agaaatccaa ctgatagtta ccaagtgaat attgggaata
    11041 aagatttccg gctccaactg tatcatcaac cagacctgtt gcctcagcta ccgttccaac
    11101 taagctcaaa agtattatca gagtgaccaa actgattaaa acaattccaa agatacgaaa
    11161 aagtttcttt ttcatctcct cactccattc tctcttgaac tggcggacgg gtatcaaaag
    11221 catggaaaag ttcttcaaag actggatgaa cttggaccac acctactcga ccatacaaat
    11281 cctgcatcaa gcattgccca ttttccaaat ctcgcatgca tttttgatta ctttcatctt
    11341 ctttatctaa tccaaagaat tccaaggttt tcttgatttc atgaatatcg gtactgcgaa
    11401 atgcaaactt tagcccaata ttattcttca aacgttcatc caacaagtca tcactgtttt
    11461 gcgtgacgaa gtaaacaccg gcattcatgg cccgaccagc acgcactaat ttattcgata
    11521 acgtttttcc ttgagctact tgaagaaaac tccaagcctc atccaggtcc acaatcttga
    11581 aaatttctcg gttgctatgg ataaagtcca aggcaaaagt ggaaataaca attagcatgg
    11641 aaacactcag caattccatg gtggtatatt cttcaaaagt ggtttctgca tctggtaaaa
    11701 ccaaatccgc tacttgaatg atatttaact gcttatctaa actaatcgac tgggtaatcg
    11761 aaccatctga gaaaagcaaa tgggcaaaat cataatcggt aaaggactcg atgtgatccg
    11821 cgatattttc cgcgactggt gagtcctctt tacgcaattc aacgattact tgtaataatc
    11881 ctcgctgatc actttgggta accgttcgaa tggctttgcg taacacaggg aacttctccc
    11941 catcccgact ggaaatccct gttaaaaagg tcaggatatc aatcgccaaa ctttctgagt
    12001 ctttcttttt cttcataatc acatagggat caagtaagcc ttgattttct ggcttactgg
    12061 tcaagttaag aatattaatt tcatccgcaa tttctggcaa cgtttctttc caaccgcctc
    12121 tttctccttt tggatcgaca atcacagctt gcccaccaaa aaggacggca taatagacta
    12181 agagattgtt gctaaaagat ttccccccac ctaacgaacc aaggaaagca gatgccaaag
    12241 cattggttac tgagcctttg accccttgtg atgccaatgc cggtttcagg taaacattgc
    12301 gccccgtatc tacgttatag ccaaagtaaa tgccttctaa ttctcctagc atttgagtag
    12361 ccccaaaacc aagtcccgcc aaaaagtcag acgtaacata ttgaatatag tcattcatgt
    12421 atcgcttact tgagggtaag aattctttgt gtagacccat catatcgcca aagggccgga
    12481 ctagttttac attggtatcg tcataaaaat cgagcacttc atcacagcgg cgttttaatt
    12541 catccactga tttagcactc actcgaacca catagcttag tttatacatg gattctttcg
    12601 actgatccaa ggtggtttcc aattcatcca cggaatccaa agcatctaat acattggagt
    12661 tcgtttcatt atcagattga taggcatggt tgtctaaatc ttttaattcc tttttcttat
    12721 tacgaacggt agctagggcc tttttattcg tcacaatttc cacattcatt gaagtatcaa
    12781 tcggaaacgt aaattgttgt tgctggtaat agaaaagttc cgaggaagga aactccattt
    12841 cccctacaat ggtattaatt gttaagtacg ctacataaga ctcatgattt tcatgttcca
    12901 tccgtaagta ccgaggcttt tcttcaatca aacaacgagt gggacgtaat aagtcgtaac
    12961 gtttcaccag cgtttctgat ttcagctttt tctttggtag ctggaattca tattcttcaa
    13021 acggtgtgcc tttttcacca tagatatgtt caattaaata cgtcaagtca ctcggcgtta
    13081 ctcgtttgac tttaaatctt cgagccaatt tactttccat tagtttttca agcttggtat
    13141 agcgacgaat ttcttcattg gataaggaaa caaaatcacc catcaaatga tgattcactc
    13201 catagacaaa ttcttgaagt ccagaaaaga aagatttcct gagattcttt agattgacct
    13261 cttcatctgt cgaaatcagt ttaaagccaa taaagaagcg ataatcaatc tgactatcgc
    13321 caatcatgga tactaatgca tctgtctgta aatcaattcg ttgctttgct acttctttga
    13381 gtctaccggt aatttccttt ttcgaacgtt cttgtgtggc acgaatacta ctttctgtgg
    13441 caatttgtaa ggcatggatc ttaccttcac gattttgcgc aattaattgg cgaaagttat
    13501 catgtacttg atacttctgt tcaggtgaca aaaaggagta attgtaaggc actaattcat
    13561 agtaagcaaa acactcgcct tcttggttaa agacaagatt attttcaata tacttaatcg
    13621 ggtacataat tcacactcct taccaaggtg attggttcgt cccatcgttt cttctgttct
    13681 ttaactttct ttcctgcaaa agtgacctta aaccgtaagt gatagctgat aaaggatcgt
    13741 aaaaatccca atggtttctt accgtcaaag gtcttttgcg acatgaacca agtgaccgct
    13801 atcggaatgc ccaagtattt taaaaaagcc ccctctatca tggatagcgg aggcacatgg
    13861 gcaaatacaa tgacaaacat caaggataaa acaaaccaag ccatctggtt aaaggtcaca
    13921 ggaaaaggca gctgaaagtc attaatcgca taaatgactt tttcaactgc ccaaatactg
    13981 gtgtagcttt ttattttttt catagtttcc tccaaaataa aaagaaggaa aaattccttc
    14041 ttaaataaaa attctatatt tcttactata ctttccattg cttgcataat ccttttgaag
    14101 aacataaagt ttaaacctac gtgatcgacc
//
LOCUS       DACDHL010000059        13835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDHL010000059 DACDHL010000000
VERSION     DACDHL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.059"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(69..1079)
                     /gene="trpS"
                     /locus_tag="IV228_001916"
     CDS             complement(69..1079)
                     /gene="trpS"
                     /locus_tag="IV228_001916"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8717799.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            1333..2070
                     /locus_tag="IV228_001917"
     CDS             1333..2070
                     /locus_tag="IV228_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8717800.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            2084..2899
                     /locus_tag="IV228_001918"
     CDS             2084..2899
                     /locus_tag="IV228_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8717801.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            2912..3559
                     /locus_tag="IV228_001919"
     CDS             2912..3559
                     /locus_tag="IV228_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8717802.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            3575..4231
                     /locus_tag="IV228_001920"
     CDS             3575..4231
                     /locus_tag="IV228_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8717803.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            4393..5214
                     /gene="racE"
                     /locus_tag="IV228_001921"
     CDS             4393..5214
                     /gene="racE"
                     /locus_tag="IV228_001921"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8717804.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            5217..6572
                     /gene="rph"
                     /locus_tag="IV228_001922"
     CDS             5217..6572
                     /gene="rph"
                     /locus_tag="IV228_001922"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8717805.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            6573..7091
                     /locus_tag="IV228_001923"
     CDS             6573..7091
                     /locus_tag="IV228_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8717806.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            7198..7698
                     /locus_tag="IV228_001924"
     CDS             7198..7698
                     /locus_tag="IV228_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8717807.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(7978..8898)
                     /locus_tag="IV228_001925"
     CDS             complement(7978..8898)
                     /locus_tag="IV228_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8717808.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            9063..10076
                     /locus_tag="IV228_001926"
     CDS             9063..10076
                     /locus_tag="IV228_001926"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8717809.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(10186..10941)
                     /gene="fabI"
                     /locus_tag="IV228_001927"
     CDS             complement(10186..10941)
                     /gene="fabI"
                     /locus_tag="IV228_001927"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8717810.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            11161..11601
                     /gene="fabZ"
                     /locus_tag="IV228_001928"
     CDS             11161..11601
                     /gene="fabZ"
                     /locus_tag="IV228_001928"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8717811.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(11716..12477)
                     /locus_tag="IV228_001929"
     CDS             complement(11716..12477)
                     /locus_tag="IV228_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8717812.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            12666..13760
                     /locus_tag="IV228_001930"
     CDS             12666..13760
                     /locus_tag="IV228_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8717813.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4426 a         2431 c         2879 g         4099 t
ORIGIN      
        1 aaaaggaata gcacagagac gagataaaaa aacaggcctt tacagcctgc ttttttagag
       61 taaatgaatt aatggaagta attgattccc attgctgatt ttacttcaga aagtgtttgt
      121 gcagctactt tagcagcttc ttcactacct ttacgtaaga tttccataac agctgaagga
      181 tctttttcca attcttctcg gcgcgcacgg atcggtgcaa attcagcttc caatacatca
      241 atgaggtatc gtttgatctt tacatcacca agtccgccgc ggcggtaatg agctttcatt
      301 tcttcaattg cttctttatc tgttccgaat acatccaagt aggtgaagac catattccct
      361 tctacttgtc caggatcttg tacatggatg tggtttggat cagtatacat actcatgact
      421 tttttctgta agacatcggc aggatcggag atataaatac cgtttcctaa cgatttactc
      481 attttgccat ttccatcgat tccaggtaat ctacccattc cttttggtgg gaatacgcct
      541 tttggttcca ccaagacttc accatatgta tgattgaagc tttgtacgat ttcctgtgtc
      601 tgctctagca ttggtttttg atcctcacca acaggtacaa gattcgcttt gaatgcagtg
      661 atatctgctg cttgagaaac aggatagata aagaatcctg taggcacgct ttcaccgaat
      721 tttttttgtt cgatttctgt tttgacagtc ggattgcgcc gaacgcgtcc tacgctgact
      781 aagttcaaat aatacatggt taattcagct aattccggaa tttgtgattg gataaataaa
      841 gtagattttg caggatctaa tcccacggct aaatagtcta aagcaacttg caagacattt
      901 gatgaaacct tctcaggatt tttagcatta tctgtcagtg cttgcatgtc agcaatcatg
      961 acaaacagtt ggttgtcttc atctgcctgc atagccactc gggtttttag agaaccgacg
     1021 taatgcccta aatgcagctt cccagttggt cgatctcccg ttaaaataat atttttcaaa
     1081 aaagacacct ctttacgatt tgtaagtctg agcacttaat atcttaccgt ttttcctgaa
     1141 aagaaacaac agttcctaag gaaaaaacaa ggaaagagga taaaataact aatagattag
     1201 aaatggaaga aatatttatg ggagaataaa cgttctaaga aactttacaa attactaaac
     1261 tatcagatat aatgtatttt attatacatc tttaaaagaa atcttactaa ataataatgg
     1321 gagtgtaatt ctatggcaat gattaaattt gatcacgtgg ataaatatta tggcaagttc
     1381 cacgcattaa aaaatatcaa tctcgaattc gagaaaggag aagttgtcgt tgtcattggt
     1441 ccttctggtt caggaaagag cacgatgctt cgctgtatca atggattaga gacgatctct
     1501 tcaggtaagc tgctgattaa tgatactgac ttgcatgata aaaagacaaa actgacagaa
     1561 gtccgaaaaa acatcggcat ggttttccaa catttcaatt tgtacccaaa taaaactgtt
     1621 ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc aagaccaggc aactgcagtc
     1681 aaaaacgcgg agaaattttt gaaaactgtc aatatgttag ataagaaaga ttcttatcct
     1741 tccatgttat ccgggggaca acagcaacgg gtggcaatcg ccagaggttt agcgatgaat
     1801 cctgagatgc tgctgtttga tgaacctact tctgctttgg atccggaaat gattggtgat
     1861 gtattagacg ttatgaaaaa attggctcat gacggtatgt caatgattgt cgtgacacat
     1921 gaaatgggct ttgccaaaga agtggcggat cgtgttatct tcatggctga tggagaagta
     1981 ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc caaatgaagt ccgagcacag
     2041 caatttatca gtaaagttat caatcattga taggaggaaa aagatgcgca ggttaaaaaa
     2101 aatacgcctg attttccttt taggcattgc ttttcttgca ctagccggat gtaaaggaaa
     2161 tgggttggca aatgaagata ttttagagcg aagcaaatct accaatgaaa tcatctgggg
     2221 tgtcaagtat gatactcgat tatttggaat gatggatatt gagagcagaa ctgtacaagg
     2281 atttgatgta gatattgcaa aagcaatcac taaaaagatc cttggtgaca atggaaaaac
     2341 agaatttgtt gaagtcactt ctaaaactcg gattccatta ttgaaaaacg gaaatatcga
     2401 tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa aaacaggttg acttttcaga
     2461 tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa ggaagccaaa tcaaaagtgt
     2521 agatgatttg aatgcttcta caacggttct agcagtcaaa ggttctacat cggctgcgaa
     2581 tatccgacag catgcaccag atgcaaaaat tttagaatta gaaaactatg cagaagcatt
     2641 tactgcttta caatctggtc agggagacgc gatgactacg gataatgcga ttttgttagg
     2701 gattgcagat gaaaatccag agtatgagtt agttggtgga acgtttacta atgagcctta
     2761 cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa gctgttaacc aagcgctaga
     2821 ggaaatgcat gcagatggca catacgataa aatctatcaa aaatggtttc caaatgaaac
     2881 tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa ttgatacaaa cttatggacc
     2941 aatctttgtc gacgggttca agatcacatt gttttcgagt gtcttagcac ttgtgtttag
     3001 tttgattatc ggaacgttaa tggcaatctt ccaattatca aagaataaag tagtgagcgc
     3061 cttagcaaag gcatatgtag aattttttag aaacattcca ttattaatca tcgtgatgtt
     3121 tttttatgtt gtcgtaccga tttattggtt taatctagat ggattccaag caggtacgat
     3181 tggattaacg atctatacat ctgcttttat tgctgaaaca gtacgttcag ggattcaaac
     3241 cgtaccaaaa ggtcaaacag aagcaggact atcttctggt tttacctatt cagaaatcat
     3301 gcgttatatt gttttaccgc aagcattcaa aatcgttatc ccgccattag gcaaccaatt
     3361 tatcaacctc gtaaaaaact catctatttt agcaatcgta gctggtttag atttgatgta
     3421 tcaaggagac ttgatcgcta gtgaaacatt caataccttc gatacgtata tcatcgttgg
     3481 gttattttac ttagttatca cgctaccgtt atcttactta atggtttatc ttgagaaaaa
     3541 atgggcggtt cgttcataga aggaggggaa atacatggat tttagcggcg ctttttcatg
     3601 gatgaatatc cgttttttat tggaaggatt aaaggttact gttgaagttt cattgttttc
     3661 gatcattttt agtttcttga tcggtggtct gacaggtgtg attcgttttg cgaatatccc
     3721 tttcttttcc aaaatccttg gtgtagtgat tgatattata cggaatcttc cattattatt
     3781 gatcattttc ttcacttact ttgccttacc tcaaatcggc attcagatga acatcttctg
     3841 gtccgctgtc gcagcattaa cgatttttga gtcagctatg ttgtcggaaa ttttccgagc
     3901 tggactcaat gctgttccaa aaggacagat ggaagcagga ttatcaacgg gactgactta
     3961 tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt aaatcaatga tcccggcaat
     4021 tgtgagccaa ttgatttcac tgatcaaaga tacttcatta gcagtaatca tttctttacc
     4081 tgaactttct catcaggcac gtattattta tggacagaac accaattatg tgcttccgat
     4141 gttcatcatc atgacactta tgtacttcgt ggtatgttac gcgctttctc tgctttccgg
     4201 ttacttggaa aagagacggt acagttatta atagatgatt cttttagcgg ttgtggcaaa
     4261 ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa agagatttcg attgttttaa
     4321 gcaagtaaga gcacttgaaa attcacgcta aaaagtacac aatagggagg atagaattta
     4381 ttgaaaaaaa ggatgatacg attgacagat aatcgcccta tcggatttat tgattcaggt
     4441 gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat taccgaatga aaatatttta
     4501 tttgtaggag acacagcacg ctgcccatat ggccctagac ccgcggaaca ggtaatccag
     4561 tatacttggg aaatgacgga ttatctggtg gagcaaggaa tcaagatgct ggtgatcgcc
     4621 tgcaataccg caactgcggt ggctttagaa gaaatcaaag ctgctctttc tattccagtc
     4681 atcggtgtga tccttcccgg tactagagcg gcagtaaaaa aaacacaaaa taaacaagtt
     4741 ggcattatcg gtacgattgg tacggtaaaa agtcaagctt atgaaaaagc actgaaagag
     4801 aaagtaccag aattgactgt gacaagtctt gcttgtccca aatttgtttc agttgtcgaa
     4861 agtaatgaat accattcatc ggtggcgaaa aaaattgtgg cagaaacatt agctccttta
     4921 accactaaaa aaatcgatac attgattttg ggatgcaccc attatccatt attacgcccc
     4981 atcattcaaa atgtaatggg agaaaatgtt caactgatcg attctggagc agaaacagta
     5041 ggtgaagtat ctatgctgtt agattatttc aatttgagca attcaccgca aaatggtcgg
     5101 acattatgcc agttttatac aactggctct gccaaacttt tcgaggaaat agctgaagac
     5161 tggcttggaa tcggacactt aaatgtagaa catatcgaat tgggaggaaa ataaggatgc
     5221 gtcatgacgg gagaaaagcc caagagcttc gcaaactgga gcttcaaaca aatgttttca
     5281 aatatccaga aggatcggta gtgatccgtt ttggtgatac aacagttatt tgttctgcaa
     5341 caatagaaaa ttctgttccc ccttttttga gagaaacagg aacaggatgg gtgacagcag
     5401 aatatagtat gctgcctcga gcaacgagta cgagaaatcg tagagaaagc agcaaaggga
     5461 aactttctgg tagaacaatg gaaatccagc gtcttattgg tcgttccttg cgtgcagtag
     5521 tagacttgga aaaattagga gaacgtagta tcatcgtaga ctgcgatgtg atccaagcgg
     5581 atggcggtac tagaacagca agtatcactg gggcatttgt ggcattgaaa ttagccattg
     5641 aaaaactttt aagagaaaaa gaattgtcag aagatccaat aaaagaacat cttgctgcag
     5701 ttagcgtagg aatcttacca gatgggactt gcgtcacaga tttggattac caagaagatt
     5761 ccgcagcact tgtagacatg aatcttgtaa tgacagaatc tggaaaattc gtggagattc
     5821 aaggaactgg agaagaagca acgtttgatg gtgaacagtt gaatgaaatg ctcttttttg
     5881 gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca tgctttgcta acagagtttg
     5941 ctcaaaatga tgagcggata gaagaaacaa aaacaatcat cattgcgaca agaaatccag
     6001 gaaaagcaga agagtttcga aatatgttca aagaagcggg atatcatgta aaaacattac
     6061 ttgactatcc tgaattgcca gatgtggaag aaactggcag cacatttgaa gaaaatgccc
     6121 gattaaaagc agaaacgatt gcacaattgc ttgatcagcc agtcttagca gatgattcag
     6181 gtttgaaagt ggatgcttta ggcggtatgc caggaatcta ttctgcacgt tttgcagggg
     6241 aacaaaaaag tgatgcgggg aataacgcca aattattata tgaattgaca gatgtgccgg
     6301 atgaaaaaag aacagcacaa ttccattgta cgttagtatt cgcggcaccc aaaaaagaca
     6361 gccttgtagt agaagcggaa tggcccggtc gagtagcccg tataccatct ggcgaaaatg
     6421 gctttgggta tgatccttta tttatcccag aaggtaagaa acaaacagca gcagaattat
     6481 cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc aatgaaaaaa ttgagtgccg
     6541 agtggaaaca atggctggaa ggagagagat agatgcggta tttagtagta agtgataatc
     6601 atggagacag agatatcctc gttgacttag tagatcgtta tcgttctgaa gtggatatct
     6661 ttttccattg tggtgattcg gaattagaag cttctgaccc attatgggat acattccaag
     6721 tggttcaagg taattgtgat tatggaccag gattcgaaca gaaaaaagtg atccaaactg
     6781 gtcaggatac agtattcatg actcacggtc atttatcgaa tgtccgattt ggtttgacac
     6841 agttagcaat cgaagctcga gctgcaactg ccgatatggt gttttttggt catacccatc
     6901 aaataggctg tgagatggat cagaaaatcc tgtttttgaa tcctggtagc atttctcagc
     6961 ctagaggccc gatccaaatc ccatcatatg ctcttatcga aagtttagat gaccaaattt
     7021 ctgttcaata ctataaccgt gcccatcagg cagttgaaga gttggccttt tctttttcga
     7081 gagagcgctg aataaaaaaa ctctaatttt taataaaaat aagctttatt ttatgaattt
     7141 tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata gaaatggagg ccatcagatg
     7201 attggaccta tagtaagaga aatgctgctg gaaaatcagg aaacgttttt gattcctgca
     7261 gaaaatgtgg caaatgttat gtatcatcat ccattgtccc atggtttact tgtgttatcg
     7321 aaggtcggtt attccaaaat tcctgtatta ggaaaagatg atcactttgt aggactagta
     7381 agtttatcag atgtggtcaa taaaatgatt gacttgcaaa caatcagcat ggacccgtta
     7441 gaaggactaa cggttgctga tgtgacagaa acagatgttc cgacaattga tgaaaattgg
     7501 gaactagaag aagtactcca tttgttagtc gattcgcctt ttttaccggt tgtcacagaa
     7561 gatcggattt tcaaaggaat cattacaaga aaagagctgc ttaaagccgt aaacttcatg
     7621 gtgcatgaat tagaacgtcg taatatattg tctccgaaaa tcgaagaatt aaaagaacaa
     7681 atcgatatcg tgagctaaag aacgagaaac taaaagataa tcaaaaaata agtagaaaaa
     7741 acagcgtacc agttttgact ttatcttgaa aaagaggctg ggacaaaagc gtttaactcc
     7801 aagaaataag aaggaattca cgaaaattgc ttttcaaatt tttgtgaatt tcagcttatt
     7861 tccgaaggag ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtgg gacaaaagtg
     7921 ttctttactt ttgtcccacg ctctttttag tgagattcgt tgcatcgttt gtccaaatta
     7981 ttttccgaca gcaattggac tgttcggaat cgtgaaacca gaagctgtca attctttgac
     8041 ataggagctc aagaactctt cttttaaggc atattgtttt ccgttcaatg cgtagcagac
     8101 cgtacgaatc gcaaaattgc tgtttccaag atccactaat ccaaagatag aaggttcagt
     8161 ttggagctct tcttggtatt tttcagctaa tgtacgattt actcgatcaa tgatctcata
     8221 gattttgtca tatccttctt caggaacaat ccgaatatct aaaagtactt gcatatttgc
     8281 acgagagaga ttgctgatcg tggtgatgtt tcgattcggg atgaaatgga cagtaccatc
     8341 cgttgctttc agctgaaggg tacggatacc aacagatacg acggttcctt caatcgttaa
     8401 gtttgctagc ttgatgtagt ccccaacatc catctgttgt tccatgataa taaaaaatcc
     8461 ggtaataaca tcactcatga acccttgggc accaagaccg attgccacac ctgcaattcc
     8521 ggcaccagcc aaaagtgatc cgatcggcac accgatagca gataaaattg catagataaa
     8581 aaagaaacca agggtataat gaaaaatagt atttaaaaga ctatgtaaag tctttaagcg
     8641 gctctcgttt agaatggctt ttttagaata ctgattaaaa gacttatcaa tcagatattt
     8701 tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa atcagatata aacttttttg
     8761 gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc caaaaccgct gaaacgcatt
     8821 tactttgctg acagcttgat ctgtcaatgt ctcgccagaa gagtctgtcg taccggcagt
     8881 tgctaataat gaaaacataa aatcctcttt tctaaaattt gattctttct gtattgtacc
     8941 aagaagtttt caaaaaagga aatagcaaaa ataactataa tgaaggaaaa aagctgaaaa
     9001 taaaatgaca aataatatgg ataatgataa aatgtggaga aaataacgaa ggtggtaagt
     9061 atatgcaaat cgattggaac aatctaggtt tttcttatat caaaactcct tggcgtttta
     9121 ttgccaaatg gaaagacgga caatgggaag aaggagaatt gacagaagat aattacctga
     9181 cgattcatga aggctctcca gcgcttcatt atggtcagca atgttttgaa ggtctaaaag
     9241 cttatcgtcg aaaagacggc aaggtcaatc tctttcgacc tgaacaaaat agtcgacgga
     9301 tgaatcaaag tgctagacga ttgctgatgc caatggtccc tgaaaagatg ttcgttgatg
     9361 ctgtcaagca agtggtaaaa gcaaatgaag attttgtgcc gccctatgga acaggcgcta
     9421 cattatactt gcgcccttta ctgattgggg tcggtgaaaa cattggtgtc catccagctc
     9481 cagaatattt atttgtcgtc ttctgtacgc cagtaggagc gtatttcaaa ggtggactaa
     9541 aaccaacgaa ttttattgta tctgattacg atcgtgctgc tcctaagggg actggtgcag
     9601 caaaagtcgg cggaaattac gcagcgagtt tacttccagg aacagaagca aaagaactgg
     9661 attactcaga ttgtgtttat cttgatccag ccacacatac gaaaatcgaa gaagtaggtg
     9721 cagcaaattt ctttggaatt acaaaagacg gaaaattcat cactccgcaa tcgccctcta
     9781 tcttaccaag tatcacgaaa tattccttgt tgcagttagc tgaagaacga ctaggactgc
     9841 aagcaattga aggagatatt ttcattgatg atctagatca atttgcagaa gctggtgcat
     9901 gtggtacagc ggctgttatt tctccaatcg gtgggattta tcacaataac aatcttcatg
     9961 tattttatag tgaaacggaa gtaggaccag taacaaaacg gttgtatgat gaattgacag
    10021 gtattcaatt tggagacata gaagcaccag aaggctggat tgaagtcgtg gaataatttc
    10081 aaagtaatga gttgaaaaaa acgtaaaaag aaaaaatcag gaagagactt taaaaagttt
    10141 tttcctgatt tttatatgtt ttactttaaa gaatgcaaca aaagtttatg aaagatggac
    10201 acctttgtca acgtagataa tatcaccgat gattccgctg gaaagtgggc tgattaagaa
    10261 agcacaagca ttaccgactt cttcgatcgt cacaccttgc tgatcaggcg tacgagattc
    10321 agataactgg atcagttttt gatagtcttt aacacctgtg acagctaatg ttttgattgc
    10381 cccagctgag attccattga cccgaattcc tttaggcgag aactcagctg ccaaataacg
    10441 gatagcagct tcaagagaag ctttcgcaat acccatcatg ttataattcg ggattgcgcg
    10501 ttctgaacct agataagtca tgctaacgat cccgctgttt ggtgcaagaa acggttgtgc
    10561 gtattttgat acagcaatta aagagtagct gctgatatct tgagctaatt gatagccgtc
    10621 tctggaaata tcagacacat ttccggacaa ttcttctttg ttggcgtaag caactgcatg
    10681 gacaaggccg tggatctccc cagcatattc accgatggca ttcattgctt tttcgatgct
    10741 ttcgtcgcta gaaacatcac attctacagt gaatgcttct tcgccgacca atttgactaa
    10801 tgattttttc attcgttcat tttggtaagt atagataatt tctgcccctt gtttcaacat
    10861 tgcttcagca caaccccaag caatgctttt tttgttagcg acgcccataa tgacgatttt
    10921 ttttccttct agaaatgaca taatgactct cctttgtttc tgataaaaat ttgatagtcg
    10981 taaaaaaata gagaataata gctaaaataa cttcttgatt tcttaattat tagtcaaaat
    11041 gttttgattg tcaaactatt ttttcttaaa ttaaaaaaag attgctaaat atttaggaat
    11101 atgatacttt gatagtcaaa gtaatgaaaa tggcaattgc ctgaaagaag gagtttaatt
    11161 atgtcttacg tattatctgc tactgaagta atggaattga ttcctaatcg ttatcctatt
    11221 tgttatattg attatgtaga tactttagaa ccaggaaaaa agatcgttgc gacaaaaaat
    11281 gtaacaatca atgaagattt tttccaaggt cactttcctg acaatccagt gatgccgggt
    11341 gcgttgattc tagaaacact ggcacaagca ggttcgatac ttatcctaaa atcagaagag
    11401 tttctaggaa aaagagctta tatcggtggg atcaataagg ccaaattccg tcaaaaagtt
    11461 gttccaggag atgtgctgaa atgtcatttt gaaatcacca aaatgaaggg atcagtcggt
    11521 acagcgttat ctgcagcata tgtagatgat aaaaaagtct gtgagtgcga gttcacgttc
    11581 atcgtcaacc aaaatgcttg actataaaac ctgatatagc aaaacaaaag gaagcttact
    11641 tgctttttat gtaaaaagaa gacgcctgaa tcctagcata cgttgtcatc tatgcaggat
    11701 tcagacgtta tttttttatt tttgttttgc ttcttgcagt atattgattt tatcgatttt
    11761 tacttcttct tttggtttgt ctgaactatc tgtttcgata tttgcaatag catccacgac
    11821 atccattcct tcaatcactt ggccgaagac agtataatgg ccatctagtg aaggataacc
    11881 gccgttttta taagcgtcga tgattgcttg aggatagtcg tcttttaaaa gaccatcatg
    11941 catatcatct gtattttgaa cgataaagaa ctggctgccg ttactgttag gatcttgtga
    12001 gcgagccata gataacgcac ctcttatatt ataaaggtgg ttgttgatct ctgtttcaaa
    12061 tccttctccc caaatgcttt cccctccagt gccatcgcct ttaggatcgc caccttggat
    12121 catgaaatct ttgatgacgc gatggaaagt cagtccgttg tagtaaccgt cttttgcatg
    12181 tgtcataaag ttttcaaccg cttttggtgc gtgttcggga aataatttga ttttgattgt
    12241 gcctttattc gtcaccattt cgaccagatc ttcattttcg gcgacatctt tcgtcaattg
    12301 tggtaattcc aatgcgttca aatcgacgct actggcagtc gtgctttccg cagtagatgt
    12361 actttttgaa ctttctgtag acgaactaga agaagtcgtg ttgtttgcag aacaaccagc
    12421 agcaaacagc aatgctgctg ctaaactaat acttaaaata actttatttt tcttcataac
    12481 tgttcctttc caatttacta ttttgttatc tttaatgata acaaaataga gggaaaatac
    12541 ctacttaaat tattggaatt tatcatttat tttaaaagaa aaagaaaagg tgtagaccat
    12601 ttactttgtg tttgctataa tgtaagtgga tacaaaaacg aaaagatgca ggaggaaatg
    12661 gtaatatggg aaaattagga atctcgattt atccagaacg gtcaacattt gaaaaggaca
    12721 aagcgtactt ggatttagct cataagtatg ggtataaacg ggtttttacc agtttgctgc
    12781 agatcaatga tgataaagaa aaagttcttt cagaatttaa agaagtagtt gattacgcga
    12841 atagcctagg catggaagta atggttgata tcaatccagc gttatttgaa cagttggaga
    12901 tttcttacga tgacttatct tttttccata agatgggtgc ctacggggtc cgcttagata
    12961 tcggattcac aggagcagaa gaagcaaaaa tgacgcgcaa tccttttggt atcaaaatcg
    13021 agatcaatat gagttcgggc acgaattacg ttgacaacat catgagttat tcgccaaaca
    13081 cggataattt attaggttct cataattttt atcctcatcg atactctggt ttaggatacg
    13141 aacactttgt tttttgttca gaaaaattca gaaaatataa tttgaatacg atggcattcg
    13201 tcaattcgca atctgctgag tttggccctt ggcctacgca agatggcttg tgcacgctgg
    13261 aagaccaccg agatcttgaa attgcgacac aagtgaagca tttaatcctt acaggattga
    13321 ttgatgatat ttctattgga aatgcgtacg caagtgaaga agaattgaaa gaaatggcag
    13381 aagcatttaa cgcggattat ccaacattga aagtagatac ggaagaaggt attacagaaa
    13441 acgaacgaat ctgcttgttt gataaccttc atagttatcg aggggatcgc tcagaatata
    13501 tccttcgttc aacgatgact cgtgtatact acaaagataa agatttcccg cctcataata
    13561 cgcgagatat gcatcatggg gatgtactga ttgacaatga agggtacggt caatacaaag
    13621 gagaaacaca aatcgccttg aaggatatga aaaacgacgg acgtgtcaat gttgtaggaa
    13681 gaatctccga tgacgaatta ttcctccttg actttttgaa accatggtca agctttaaat
    13741 taattgaaaa taaaaaatag ataattagtt attgcagaaa taatccgcta gaaatagcca
    13801 gttatttctg cttttttatt ttgttttttt atagg
//
LOCUS       DACDHL010000060        13812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDHL010000060 DACDHL010000000
VERSION     DACDHL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.060"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2158
                     /gene="acm"
                     /locus_tag="IV228_001931"
     CDS             <1..2158
                     /gene="acm"
                     /locus_tag="IV228_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8717814.1"
                     /translation="CRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTN
                     ITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLT
                     VRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVT
                     KPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPS
                     TFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMV
                     SYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKI
                     FKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKE
                     APNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYF
                     KLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYV
                     PSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKE
                     VSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIP
                     GSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSK
                     SILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2430..2915)
                     /locus_tag="IV228_001932"
     CDS             complement(2430..2915)
                     /locus_tag="IV228_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8717815.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3395..4036
                     /locus_tag="IV228_001933"
     CDS             3395..4036
                     /locus_tag="IV228_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8717816.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4055..4690
                     /locus_tag="IV228_001934"
     CDS             4055..4690
                     /locus_tag="IV228_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8717817.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4708..5523
                     /locus_tag="IV228_001935"
     CDS             4708..5523
                     /locus_tag="IV228_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8717818.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5842..7278
                     /locus_tag="IV228_001936"
     CDS             5842..7278
                     /locus_tag="IV228_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8717819.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7467..9914
                     /locus_tag="IV228_001937"
     CDS             7467..9914
                     /locus_tag="IV228_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8717820.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10144..11211
                     /gene="rlmN"
                     /locus_tag="IV228_001938"
     CDS             10144..11211
                     /gene="rlmN"
                     /locus_tag="IV228_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8717821.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11404..12507)
                     /locus_tag="IV228_001939"
     CDS             complement(11404..12507)
                     /locus_tag="IV228_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8717822.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13059..13607
                     /gene="aac(6')"
                     /locus_tag="IV228_001940"
     CDS             13059..13607
                     /gene="aac(6')"
                     /locus_tag="IV228_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8717823.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4722 a         2306 c         2924 g         3860 t
ORIGIN      
        1 gtgtagaaat attattttat caatgttatt tattattact aatataacat cactgattcc
       61 tgttcatgtc tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt
      121 ggacccaacg aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa
      181 aaaacaaaat atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat
      241 tcgcggtgaa ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac
      301 tttaacggtc agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga
      361 ctcagtagaa ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga
      421 agaaaatacg ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac
      481 aaagccagct tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc
      541 tgaagatact aagcacattc gatggttttt gaatattaac aataacggca cttatgttga
      601 acaaccagtt aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt
      661 tgaaattaat caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca
      721 acaattttta caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat
      781 tgaaataact ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac
      841 gatcatagaa aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga
      901 atttaataaa cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc
      961 ttctggtgga gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga
     1021 cacagagatt ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat
     1081 tcaaggacag tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg
     1141 cctgcaagta ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga
     1201 tccactaagt tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag
     1261 tttacctatt tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg
     1321 tggaaccact ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg
     1381 ggaaccagtt cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg
     1441 ggaagatatc caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt
     1501 agatcaaaac ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt
     1561 cgttacaaat gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa
     1621 tgagaaccaa gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca
     1681 accgatccaa cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct
     1741 gccaaaatac agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga
     1801 atacagtacg acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa
     1861 tatatctata ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag
     1921 agagaaaatt agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc
     1981 cagcgaacaa agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa
     2041 atcaaaaagc attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg
     2101 tttattgata gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt
     2161 agaatataat attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata
     2221 atcgaaaaaa gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat
     2281 ttggctaact atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt
     2341 gtttgattgg ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg
     2401 aattgggaaa taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct
     2461 aagtaaacaa gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata
     2521 tttggatgaa agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt
     2581 tttttctctt tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg
     2641 aggtcaggaa gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct
     2701 agttgtttta aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct
     2761 tttcttgatt tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt
     2821 ttgactgacg taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt
     2881 agttcataag cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt
     2941 agtgtatcat aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt
     3001 aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg
     3061 gtggaaagag gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat
     3121 agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc
     3181 tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat
     3241 cttcggatca aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc
     3301 tgcaaggaaa aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca
     3361 agaggatttt tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc
     3421 gcaattatta gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc
     3481 attaccatta gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta
     3541 cttgcttcaa acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt
     3601 tattttttat gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat
     3661 tgcctttatc ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc
     3721 gattcctcaa ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt
     3781 gacgaaaatc gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt
     3841 tatcaacttg gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg
     3901 tgctgggaaa atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat
     3961 catctatctt acattgactg gtattctaac aatcgtaatg aaacaagttg aaggaaaatt
     4021 aaattattac cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa
     4081 agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac
     4141 attagctatc gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg
     4201 cttagaaagt tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga
     4261 gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca
     4321 tctgaatatt tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga
     4381 agaatacacc aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa
     4441 tatctatcct tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt
     4501 ggctatgaat ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt
     4561 acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat
     4621 tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc
     4681 aattcattaa gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc
     4741 gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg
     4801 actatcaacg aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga
     4861 tttagagata aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt
     4921 gaacaatatg ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa
     4981 ctgaacaatg gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa
     5041 aagaaagtgc aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa
     5101 gaatcaggaa tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg
     5161 tcatctggtt atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac
     5221 caagatgctg ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg
     5281 gtcgatggtc tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa
     5341 ctagaagact tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca
     5401 cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa
     5461 acaggtgaat tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat
     5521 taacaaatag gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg
     5581 ttctcctgcc aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa
     5641 cggtgttcaa aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc
     5701 attttcacaa aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac
     5761 ataattttat tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg
     5821 ttataggaaa ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt
     5881 ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa
     5941 ggagaatcag tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac
     6001 ggtgatttgg cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa
     6061 caaggtctga aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga
     6121 ggagaagtca atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa
     6181 aacgatattg aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat
     6241 gaatataatg gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca
     6301 ttatttgagg cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc
     6361 ttcacttcat taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga
     6421 atgtatgaag ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac
     6481 gagttagaaa tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt
     6541 gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat
     6601 tggatggatg tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa
     6661 aaaggatggg caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca
     6721 gattttcttg ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga
     6781 gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga aactggagtt
     6841 cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag
     6901 attgacccag aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg
     6961 gtactgatta cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc
     7021 catgacgact atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt
     7081 tcggatggag cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg
     7141 ctgaacggtt atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa
     7201 ggatctttgg aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa
     7261 aatggagaga acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt
     7321 gccttttctt gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg
     7381 aaagaaagcg tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg
     7441 aaacttgatg aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga
     7501 aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg
     7561 ttgggtttgc cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa
     7621 tattgattgc aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt
     7681 tattagccgt attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa
     7741 accgacgctg gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc
     7801 atttatttgg caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt
     7861 tagccagtga tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag
     7921 caatccttta tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag
     7981 tgctattact ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa
     8041 atggtatcca atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc
     8101 aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag
     8161 caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa
     8221 aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag
     8281 agccggagca gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga
     8341 atctagagaa accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag
     8401 atgagttgcc agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa
     8461 atcaagacta tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa
     8521 gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt
     8581 tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag
     8641 tccagccggc tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc
     8701 ttgctttagc ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg
     8761 gtattgaagt accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac
     8821 aaccaaacca cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg
     8881 tacaaacggc tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg
     8941 ggaagtcagt agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg
     9001 aagtaaaact gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac
     9061 ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag
     9121 tagtacaaga aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt
     9181 ctggttacaa tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct
     9241 taccattcat cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg
     9301 aagttgaaga tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga
     9361 tcctagctac tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac
     9421 cttccagaat ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta
     9481 atggggcaga aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata
     9541 aaccgatccg tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat
     9601 ttgttacaga tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg
     9661 aaacgacagg tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg
     9721 tcgttgaaat gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca
     9781 accgagcagc acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag
     9841 gaagcaagcc tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg
     9901 ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac
     9961 aaggcatgat gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga
    10021 tgtcttgtct ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt
    10081 gcattaagga aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat
    10141 actttggaaa aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata
    10201 gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt
    10261 gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag
    10321 cattttatca tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta
    10381 aaatatttgt ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa
    10441 tatggcttgt cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt
    10501 gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc
    10561 atgatggtcc aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt
    10621 gtaatgggga tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc
    10681 atcaatgatg ccaaaggatt ggcaattggt gctcggcaca ttacagtttc cactagtgga
    10741 ttagcacaca aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc
    10801 ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca
    10861 attgagaaat tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg
    10921 tttgaatata tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca
    10981 gatctattaa aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc
    11041 agtgaacatg atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta
    11101 ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca
    11161 gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct
    11221 actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt
    11281 aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct
    11341 catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat
    11401 tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac
    11461 gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc
    11521 atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat
    11581 gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa
    11641 atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg
    11701 aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc
    11761 agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga
    11821 aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt
    11881 accataagtc aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt
    11941 gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc
    12001 gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa
    12061 ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata
    12121 gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac
    12181 tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac
    12241 tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc
    12301 ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc
    12361 tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt
    12421 tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata
    12481 atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt
    12541 aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag
    12601 acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa
    12661 aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat
    12721 ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt
    12781 atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat
    12841 ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc
    12901 tattttgaat aaagtgtttc tgtataattt tttattttat aagtaagata aataatgaaa
    12961 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
    13021 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
    13081 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
    13141 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
    13201 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
    13261 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
    13321 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
    13381 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
    13441 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
    13501 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
    13561 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
    13621 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
    13681 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
    13741 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca
    13801 tttttgcata aa
//
LOCUS       DACDHL010000061        13696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDHL010000061 DACDHL010000000
VERSION     DACDHL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.061"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            496..1098
                     /locus_tag="IV228_001941"
     CDS             496..1098
                     /locus_tag="IV228_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8717824.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1114..1791
                     /locus_tag="IV228_001942"
                     /pseudo
     CDS             1114..1791
                     /locus_tag="IV228_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2303..2599
                     /locus_tag="IV228_001943"
     CDS             2303..2599
                     /locus_tag="IV228_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP8717825.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2600..3016
                     /locus_tag="IV228_001944"
     CDS             2600..3016
                     /locus_tag="IV228_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8717826.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3036..4580
                     /locus_tag="IV228_001945"
     CDS             3036..4580
                     /locus_tag="IV228_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8717827.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4723..4947
                     /locus_tag="IV228_001946"
     CDS             4723..4947
                     /locus_tag="IV228_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8717828.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            4962..5831
                     /locus_tag="IV228_001947"
     CDS             4962..5831
                     /locus_tag="IV228_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8717829.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5812..6546
                     /locus_tag="IV228_001948"
     CDS             5812..6546
                     /locus_tag="IV228_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8717830.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6579..7487
                     /locus_tag="IV228_001949"
     CDS             6579..7487
                     /locus_tag="IV228_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8717831.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7484..8026
                     /gene="sat4"
                     /locus_tag="IV228_001950"
     CDS             7484..8026
                     /gene="sat4"
                     /locus_tag="IV228_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP8717832.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8119..8913
                     /locus_tag="IV228_001951"
                     /note="aph(3')-IIIa"
     CDS             8119..8913
                     /locus_tag="IV228_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8717833.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9021..9209)
                     /locus_tag="IV228_001952"
                     /pseudo
     CDS             complement(9021..9209)
                     /locus_tag="IV228_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9189..9761
                     /locus_tag="IV228_001953"
     CDS             9189..9761
                     /locus_tag="IV228_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8717834.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            10101..10955
                     /locus_tag="IV228_001954"
     CDS             10101..10955
                     /locus_tag="IV228_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8717835.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            11054..11263
                     /locus_tag="IV228_001955"
     CDS             11054..11263
                     /locus_tag="IV228_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8717836.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11280..11552
                     /locus_tag="IV228_001956"
     CDS             11280..11552
                     /locus_tag="IV228_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8717837.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            11554..12417
                     /locus_tag="IV228_001957"
     CDS             11554..12417
                     /locus_tag="IV228_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8717838.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(12680..13417)
                     /gene="erm(B)"
                     /locus_tag="IV228_001958"
     CDS             complement(12680..13417)
                     /gene="erm(B)"
                     /locus_tag="IV228_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8717839.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(13542..13625)
                     /locus_tag="IV228_001959"
     CDS             complement(13542..13625)
                     /locus_tag="IV228_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8717840.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         4853 a         2128 c         2848 g         3867 t
ORIGIN      
        1 tatgatacgt gtttgttttt ctgtgctgtt tagtgaatga ttagcagaaa tatagagagt
       61 aagattttaa ttaattatta gggggagaag gagagagtag cccgaaaact tttagttggc
      121 ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg aggggcagtg agagcgaagc
      181 gaacacttga tcttttaagt tgctatcttt ttaaggtcaa tagagtatac ttatttgtcc
      241 taatatggtt ttagcagtat aattgacttg gtgaataggt catttaagtt gggcataata
      301 ggaggagtaa aatgaaaaaa tttatttatc gagttttaga aaatgacgaa gtggtggcta
      361 tttttaatga gcaacaatat gcgcaagatt ttatcgctta cgaaaagaca atttctgata
      421 agcaatttga aattgaaaaa gtagatatag ctgattggtt attgcaaccg agagaatttt
      481 agaggttggt tgaaaatggc taaaattggt tatgcacgtg tcagtagcaa agaacagaac
      541 ttagatagac aactgaaagc gttagagggc gtttctaagg tcttttcaga caaattaagc
      601 ggtcaatcgg tcgaacgccc acaattacaa gctatgctta actatattcg tgaaggggat
      661 atagttgttg ttactgaatt agatcgatta ggacgaaata ataaagaatt aacagaattg
      721 atgaatcaaa ttcaaattaa gggggcaacc ctggaagtct taaatttacc ctcaatgaat
      781 ggtattgaag atgaaaattt aagacggctg attaataatt tagtgattga attgtataag
      841 taccaagcgg aatctgaacg caaacgaatt aaagaacgcc aagcccaagg aattgaaatt
      901 gctaagaaaa aaggaaaatt caaagggcga caacctaaat tcaaagaaaa tgatccacgt
      961 ttacaacacg ctttcgattt atttttgaat ggttgttccg ataaagaagt tgaagaacaa
     1021 actggaatta atcgccgaac gtttagaagg tatcgatcaa gatacaacgt gacagtagat
     1081 caaagaaaaa aacaatgaaa agagggatag ttaatgagta cggttatttt agctgaaaaa
     1141 ccaagccagg cattagccta cgcaagtgct ttaaaacaaa gcaccaaaaa agacggttat
     1201 tttgagatca aagacccact atttacagat gaaacgttta tcacctttgg ttttgggcat
     1261 ttagtcgaat tagcagaacc aggtcattat aacgaaaagt ggcagaactg gaaacttgaa
     1321 tctttgccga tttttcctga tcgatacgat tttgaagttg caaaagataa gggaaagcag
     1381 tttaaaattg ttgctgaact tctcaaaaag gcaaatacaa ttattgtcgc aacagatagc
     1441 gacagagaag gtgaaaatat cgcctggtcg attatccata aagcaaatgc cttttctaaa
     1501 gataaaacgt ataaaagatt atggatcaat agcttagaaa aagatgtgat ccgaagcggt
     1561 tttcaaaatt tgcaaccagg aatgaattac tatccctttt atcaagaagc gcaaacacgc
     1621 caaattgccg attggttgat cggcatgaac gcaagccctt tatatacctt gaatttacaa
     1681 caaaaaggcg tacaaggtac attttcatta ggacgtgtac aaacaccgac tttatatctc
     1741 atttatcaac gccaggaagc aatagaaaac tttaaaaaag aacctttttt cctgaataat
     1801 tcatagtttt actaatagtc tttcctaaca ttgatttaat agtctttaaa gtattctgct
     1861 tcatcaccca ttaggtgatg aaaaaagaac ccctttctga tttagcggac aaatactttg
     1921 acctaatctc agaagatata aaagaaaaaa taataaaata tccgatttat actttgtata
     1981 aagaactaaa gtagttagca aatcatttac agcaaatcac tatgttccac ataccatttt
     2041 acatacttac cttatccacg acacatgttg agatagtatc gaagataaag aaagttgctc
     2101 catgttgttt ttgcgagatg gggaaatcaa agccacattg acaaccatga tgaataagct
     2161 tgctttcagt cataagctaa tcctagaacc acttttcaag tcagttagcc aaatcgatga
     2221 agaaagcgac cgtgaaagaa tggacgctat tgataagcta atggagcaac tattggaaga
     2281 acgcaatacc cttattgcac tgatgtccaa aggtttccta gaacctgctc tttttaatca
     2341 ggaacgaaat gttttagata gtgagattaa aaatcttact accgaaaaaa caaacctggt
     2401 aacgaactct gcgagtgggg ttttgcgagc aaacgagata aaggacctca ttaattatgt
     2461 gtcagcagat aattttaatg gtgactacac ggaagaacta tttgaagaat ttgtagtgaa
     2521 catcattgta aattccaggg atgagctgac attcaatttg aaatgcggtc tttccctgaa
     2581 agaaaaggtg gtgagataaa tggcatatat tccatatgga tacaaaattc aagatggagt
     2641 ggttactgtc gatgaaaagg cagcaggtca agtaaaggta ttctttgaga aatacatatc
     2701 aggactatcc cttacagtgg ctggcgaaca ggcaggtatt gaaaagacac attctgtgat
     2761 ggggcgcatt ttgaaaaacg tcaactacct tggaaatgat acgtatccag caattattga
     2821 taaagagata tttgataaag ctgaagaagt tagagataaa cgtgcaaagg atttaggacg
     2881 agtggtagag cttgccgctt tcacctctcc ccctcccaaa gaacgattca aaatgaaaaa
     2941 ggcagataat aagatgccag tggatccttt tgaacgagca gaatacttat acagtctgat
     3001 agaaagcgag gaataaagtg acagagaaaa atataatggt tattcctgct cgtaaaagag
     3061 taggaagtac agccgcaaaa gaaaagataa agaaacttcg tgttgctgcc tattgccgtg
     3121 tttctacaga aacagaagaa caaaattcta gctatgaggt tcaggtctca cactatacag
     3181 agtttataaa gaaaaataat gaatgggagt ttgctggcat ctttgcagat gatggtatct
     3241 ctggtacgaa cactaaaaag cgtgacgaat ttaatcgcat gattgcagag tgtatggacg
     3301 gtaacatcga catggttatt acaaaatcca tcagccgatt tgcgcgtaac accctagact
     3361 gccttcaata cattagacag ctcaaggata agaacatatc agtttatttt gagaaagaga
     3421 acatcaacac cacggatgcc aaaggtgagg ttttgctgac tattatggca tctttggcac
     3481 agcaggaaag ccagagcctt tcacaaaacg ttaagcttgg gctacagtac cgataccaac
     3541 aaggaaaggt gcaggtcaac cataagcgat ttatgggcta cacgaaagat gaagatggaa
     3601 atttaactat tgtccccgaa gaagctgaga ttatcaaacg catctaccga gaataccttg
     3661 agggtcagag tctagtgggc attggtcgag cccttgaaaa ggatggtatt ttaacagcag
     3721 cgggaaaacc aagatggcga ccagaatcag ttaagaagat acttcaaaac gaaaaataca
     3781 tcggagatgc ccttctgcaa aagactgtca cagtagattt tctgaccaag aaacgagtga
     3841 aaaatgaagg gcatgttccc caatattatg ttgaaaatag ccatgaagcg attattccta
     3901 aagagttatt tttgcaggcg caagaagaaa ttcatcggag aagcaatatc tacacaggag
     3961 aaggcaagaa caaaagaatt tacagtagca agtacgcttt aagtgccatc accttctgtg
     4021 gagattgtgg cgatatttat agacgggtct attggaatat ccacggaaga aaagaatttg
     4081 tctggcgatg cgtgactaga atcgagcaag gtcctgaagt ttgtaagaac cgaaccgtaa
     4141 aagaagatga actttatggt gcagtaatga ctgcgattaa taagctactt gcaggtggga
     4201 acaatatgat aaagacactg gaagagaata ttcatgctgt gattggcgaa accacagaat
     4261 accaaatttc agagattaac aactcactag aggaaaaaca aaaagaacta atcaagctgg
     4321 cgaacaaggg tcaagactat gaccacttag cagatgagat tgatgagctg agagacaagc
     4381 gacagatcct tttagtagaa gatgcctccc tcagtggcga gaacgagcga atcaatgagc
     4441 tgattgaatt tatccgcaag aacaaattcc gcaccttaga atatgatgat aagcttgtaa
     4501 ggaagataat ccagagcgtt acagtctatg aaggccactt cgtcatagcc ttcaaatctg
     4561 gcatcgaaat ggaaatatga aaaccagaag taaatagccc atgactctac agtagagttg
     4621 tgggttttct tttgcttgct gattataata atattttaat aactattgtg tttatcaacc
     4681 aaatggttta taatattctt agcgtaaact tggaggtgct gtatgactac ggcagaaatg
     4741 attaaagaac tgtgtgagca aatgaatata agtgtttccg aacttgctag acgtattggc
     4801 cagactccac agaatttcaa taaaaaatta caacgagaaa cagtaacctt ggatgagttg
     4861 aaggccattg ctgatgtgct aggtgtcaag tttgtgcagg catttatttt accagatgga
     4921 gaagaaataa aaatatctaa cgaataaagg aggatgttta tatggtaggt aatgttattc
     4981 aaatagtaac agagaaatta tcatctttgc cttttataga aggcatcgta ttagggggct
     5041 cacgtgcaag aggcacccat acagaaaatt ctgatataga tatcggaatc tattacaatt
     5101 cagactcatt tgacctgaca gcgattaatc aaattgctac agagttggat gatgagaata
     5161 gaaacaacct tgttgtacct cccggagcgt ggggtgattg ggttaatggc ggtggatggt
     5221 tagttataaa cgggtgtcat gttgacttga tattacgtga tataaaacgg gtggaacaaa
     5281 taatcaaaga tacggagcaa ggaattgtta ctgccaatta tcagaccggg catccccatg
     5341 gttatataag tgctatgtat cgtggagaat tagcgattag taaaatacta tatgctaaga
     5401 acgaaagctt atgcgaatta aaaaagcagg cagaaattta ccctactgct ctaaagaaaa
     5461 gcttgatgaa cttttttata tttgaagctg aattctcttt aatgtttgta aaagcaaatg
     5521 cgggggcaga ggataaatat tatattgcag gccatgtttt tcgtattatt tcatgcttaa
     5581 atcaagtact atttgcatgt aataatgctt attgcattaa cgaaaagaaa gctataaaac
     5641 tgcttgaaac ttttgaatat aaacctgaaa aatatgccga aagagttaat catatttttg
     5701 aagtactcgg tctttctctt tttgaatgct acgacatgac cgagaaactt tataaagaag
     5761 tgaatgaaat tgtatcggag ataaataact ttttaaacga ggagagttca gatgaaagaa
     5821 aacaaatatg atgataatat attttttcaa aaatacagtc aaatgagtcg ctcgcagaaa
     5881 ggactggctg gtgcgggaga atgggagact ttgaaaaaga tgctacctga ttttaagggt
     5941 aagcgtgtgc ttgatttagg atgcggctat ggatggcact gtatatatgc gatggaaaac
     6001 ggtgcttcct ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt agcaaaagga
     6061 aaaacccatt ttccacagat tgaatatgaa tgctgtgcca tagaagatgt ggatttccca
     6121 gaggagagct ttgatgtaat actaagttcg cttgcgtttc attatgtagc agactatgag
     6181 aatttaataa aaaagatata taggatgctg aaggctggtg gcaatttagt ttttacagtt
     6241 gaacatcctg tttttactgc tcatggaaca caagactggt attataacga aaaaggagaa
     6301 atactgcatt tcccggtgga caattattat tatgagggca aacggacagc tatgtttttg
     6361 gaagaaaagg ttacaaaata tcatagaaca ctgaccacat atctaaatac actgctttca
     6421 aatagtttta taataaatca gattgtggag ccacagccgc cagagaacat gatggatatt
     6481 ccggggatgg cggatgaaat gcgacgccca atgatgctga ttgtatcggc aaaaaagaag
     6541 atgtaataat atagaaaaaa taaacgagga gtatgtaaat gagatcagaa aaagaaatga
     6601 tggatttagt actttcttta gcagaacagg atgaacgtat tcgaattgtg acccttgagg
     6661 ggtcacgcgc aaatattaat atacctaaag atgaatttca ggattatgat attacatatt
     6721 ttgtaagtga tatagaaccg tttatatcta atgatgactg gcttaatcaa tttgggaata
     6781 taataatgat gcaaaagccg gaggatatgg aattattccc acctgaagaa aagggatttt
     6841 cctatcttat gctatttgat gattacaata aaattgatct taccttattg cccttggaag
     6901 agttagataa ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta
     6961 gaattaaaag ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa
     7021 gggagtatga tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat
     7081 tgtgccgtaa ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc
     7141 tgctgagaat gatatcatgg aaggtcggca tcgaaacagg ctttaaatta agtgtaggca
     7201 agaactataa gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca
     7261 cctaccggat ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt
     7321 tcagggcggt atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata
     7381 ggaatataac aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc
     7441 tggatagcac atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa
     7501 agcagggcac ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat
     7561 tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata
     7621 tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata
     7681 tattgacaat actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa
     7741 agttaaactg cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa
     7801 ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa
     7861 gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa
     7921 attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     7981 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     8041 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     8101 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     8161 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     8221 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     8281 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     8341 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     8401 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     8461 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     8521 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     8581 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
     8641 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     8701 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     8761 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     8821 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     8881 aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt ctaaaaagct
     8941 ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa ctgtccatac
     9001 tctgatgttt tatatctttt ctaaaagttc gctagataga gttctatatt agagatgtaa
     9061 agagttttgg tgaagaggtt tgtgagtatg gcttttataa ccagggtgca atgagaagca
     9121 ctaacagcag ctagcttaag aacgcatagg atatactttt tatggaagtc acagaggagg
     9181 aataaaattt gaacaggatt aacagagtaa cctctattct tattcagctg caatcaaaaa
     9241 aaataattcc tgccaaagaa attgcacagc gatttaatat aagcttaagg acagtttaca
     9301 gagatatccg gacacttgaa gaagccggaa taccaattgg atctgaggcc ggaaaaggat
     9361 attttcttgt agagggcttc ctacttccgc cggtaatgtt tacagcggcg gaagtgggtg
     9421 cattgattac agcagggaaa tttttaaatt gccatggaga tgaatcattt ataaaggatt
     9481 ttgattcagc tatgtataaa atcaaatcta ttttgaagca tggtgaaaaa aactatgcac
     9541 aggagttgga gaacagtatt aacgtgtaca gcacatctgg acagaaaaat acattggcgg
     9601 ataacgttat tgcagcaatt cagactgcaa tctgcaataa aagggtaata tcaattcaat
     9661 atcctgcatc aggagggcaa gaaccggaaa gcagaatgat agagcctata tcactggggt
     9721 tttatgaaca gaactggtac ttaatcggtt ttgcaggcta agaaatgaat ataggaattt
     9781 tcgtgtggat cggattaaaa gcatttgcat agaagaagag ctctgtcaaa gccatgatgg
     9841 ctctttggaa caaatattaa aacaaatgct ttcatataaa aaactatatg ccctacaatg
     9901 ccgttgtaaa aattggggaa aagggatata ttgattttat atctttctca aaataaatat
     9961 aaaagccctt taaagagggc ttttatatat taatcacaaa tcacttatca caaatcacaa
    10021 gtgattaatc acaaatcact tgtgatttgt gattcttaat gatacaatat tactatacaa
    10081 aaaaagaatg gggcgtagtt atggagaagg aaaaactaaa aatacttgaa gaattaagac
    10141 gtattttaaa caataaaaat gaagcaatta ttatcttgaa taattacttt aaaggtggtg
    10201 ttggaaagtc caaattatcg actatgtttg cttacttgac agacaaattg aatttaaaag
    10261 ttttaatgat cgataaggac ttacaggcaa cattgacaaa agacttagca aaaacttttg
    10321 aggtagaatt gccacgtgtc aatttttatg aaggcttgaa aaatggaaac ttggcttctt
    10381 ctattattca tttgactgat aatttagact tgatccctgg cacgtttgat ttgatgttac
    10441 tgccaaaatt aactcgctca tggacgtttg aaaatgaaag tagattgctt gctactcttt
    10501 tagcaccttt aaaaagtgac tatgatctta ttattattga tactgtacca acgccaagcg
    10561 tttatacaaa taatgcaatc gtggcgagtg attacgttat gatcccttta caagcagaag
    10621 aagaaagtac aaacaacatt caaaactata tttcctattt gattgatttg caagaacagt
    10681 ttaaccctgg actagatatg attggttttg ttccttattt agttgatacg gacagcgcaa
    10741 cgataaaatc aaacctggaa gaactgtaca aagaacataa agaggataat ttggttttcc
    10801 gaaatattat caagcgaagt aataaagtaa gtacttggtc taaaaatggc attacagaac
    10861 acaaaggcta tgacaaaaaa gttttgtcta tgtatgagaa cgtatttttt gaaatgattg
    10921 agcgaatcat tcaattagaa aatgaaaaag aatagaatca caaatcacaa gtgattaatc
    10981 acaaatcact tgtgatttgt gataggtgat gataaaataa atagtaagaa gaaatagaaa
    11041 gaagtgagcg atcgtgggaa atttaggcgc acagaaagca aaacgaaatg atacgccaat
    11101 cagcgcaaaa aaagatataa tgggggataa gacggttcgt gttcgtgccg acttgcacca
    11161 tatcataaaa atcgaaacag caaagaatgg cggaaacgta aaagaagtta tggatcaagc
    11221 cttagaagaa tatatacgga aatatttacc tgacaaactt taaaaggagt gaaaatgaaa
    11281 tggcagttac gtatgaaaaa acatttgaaa ttgaaatcat taatgagtta tcggcaagtg
    11341 tttataatcg ggtattaaat tatgttttga atcatgaact agatactaaa aatactcgtt
    11401 tactagaagt gaatctttta aatcaattag aagtggcaca agaagttgat ttatttcaac
    11461 aaccatttga agaattacaa gctattcatg agtattggcg gtcaatgaat caatattcaa
    11521 aacaaatttt gaataaagag aaagtggctt aacatggcga atattactga cttcaccgaa
    11581 aagcaatttg aagatcgttt agaaaagaat gttgaacgac taactaaaaa tagactagcg
    11641 gttgaatcgc caaccgcttt tttacttggt gggcaaccag ggtcagggaa aaccagtttg
    11701 cgatcagcaa tttttgaaga aacacaaggg aatgttattg tcattgataa tgataccttt
    11761 aaacaacagc acccaaattt tgacgaatta gtgaaactct atgaaaaaga cgtagtaaaa
    11821 cacgttaccc cttattcgaa tcgcatgaca gaagcactta ttagccgttt aagcgatcaa
    11881 ggctataatt tagtgattga aggaacagga cgaacaacag acgttcccat tcaaacagcc
    11941 acaatgcttc aatctaaagg ttatgaaaca aaaatatacg tcatggcagt accgaaaatt
    12001 gaatcatact taggaacgat tgaacgatat gaaaccatgt atgcagatga tccaatgaca
    12061 gctagggcaa caccaaaaca agcgcatgat attgttgtca aaaacttacc gaccaattta
    12121 gaaacccttc ataaaacggg cttatttagc gatataaggc tttacaatag agaaggagtg
    12181 aaactctatt caagcttaga aacgccttcc attagtccga aagagacctt agaaagagaa
    12241 ttaaatcgta aagtatcagg gaaagaaatt caaccgactt tggagagaat agagcaaaaa
    12301 atggttcaaa atcaacacca agaaacccct gaatttaaag caattcaaca aaaaatggaa
    12361 agcttgcagc tacctacacc accaataccc aaaacaccta aacttccagg tatttaaacc
    12421 ttaaaaaagg aaaagagtag ttaccaaaaa acggtaacta ctcttttttt ataaaaacat
    12481 ttcctcatgt tggggtcccg agcgcctacg aggaatttgt atcgataaga aatagattta
    12541 aaaatttcgc tgtttttttc taatttcatt ttgaggtgaa ttaattggta gttattagag
    12601 gtgccctata cctaataatt tatctacatt ccctttagta acgtgtaact ttccaaattt
    12661 acaaaagcga ctcatagaat tatttcctcc cgttaaataa tagataacta ttaaaaatag
    12721 acaatacttg ctcataagta acggtactta aattgtttac tttggcgtgt ttcattgctt
    12781 gatgaaactg atttttagta aacagttgac gatattctcg attgacccat tttgaaacaa
    12841 agtacgtata tagcttccaa tatttatctg gaacatctgt ggtatggcgg gtaagtttta
    12901 ttaagacact gtttactttt ggtttaggat gaaagcattc cgctggcagc ttaagcaatt
    12961 gctgaatcga gacttgagtg tgcaagagca accctagtgt tcggtgaata tccaaggtac
    13021 gcttgtagaa tccttcttca acaatcagat agatgtcaga cgcatggctt tcaaaaacca
    13081 cttttttaat aatttgtgtg cttaaatggt aaggaatatt cccaacaatt ttatacctct
    13141 gtttgttagg gaattgaaac tgtagaatat cttggtgaat taaagtgaca cgagtgttca
    13201 gttttaattt ttctgacgat aagttgaata gatgactgtc taattcaata gacgttacct
    13261 gtttacttat tttagccagt ttcgtcgtta aatgcccttt acctgttcca atttcgtaaa
    13321 cggtatcggt ttcttttaaa ttcaattgtt ttattatttg gttgagtact ttttcactcg
    13381 ttaaaaagtt ttgagaatat tttatatttt tgttcatgta atcactcctg aagtgattac
    13441 atctataaat aaatacagaa gttaaacgat ttgtttgtaa ttttagttat ctgtttaaaa
    13501 agtcataaga ttagtcactg gtaggaatta atctaacgta tttatttatc tgcgtaatca
    13561 ctgtttttag tctgtttcaa aacggtagat gttttatcta cattacgcat ttggaatacc
    13621 aacatgacga atccctcctt cttaattaca aatttttagc atctaattta acttcaattc
    13681 ctattataca aaattt
//
LOCUS       DACDHL010000062        13678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDHL010000062 DACDHL010000000
VERSION     DACDHL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.062"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..901)
                     /locus_tag="IV228_001960"
     CDS             complement(<1..901)
                     /locus_tag="IV228_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8717841.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="IV228_001961"
     CDS             1184..2152
                     /locus_tag="IV228_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8717842.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="IV228_001962"
     CDS             2265..2543
                     /locus_tag="IV228_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8717843.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2661..2897
                     /gene="secG"
                     /locus_tag="IV228_001963"
     CDS             2661..2897
                     /gene="secG"
                     /locus_tag="IV228_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8717844.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3009..3755
                     /locus_tag="IV228_001964"
     CDS             3009..3755
                     /locus_tag="IV228_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8717845.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3758..6115
                     /gene="rnr"
                     /locus_tag="IV228_001965"
     CDS             3758..6115
                     /gene="rnr"
                     /locus_tag="IV228_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8717846.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6137..6601
                     /gene="smpB"
                     /locus_tag="IV228_001966"
     CDS             6137..6601
                     /gene="smpB"
                     /locus_tag="IV228_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8717847.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6903..7622
                     /gene="atpB"
                     /locus_tag="IV228_001967"
     CDS             6903..7622
                     /gene="atpB"
                     /locus_tag="IV228_001967"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8717848.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7675..7890
                     /gene="atpE"
                     /locus_tag="IV228_001968"
     CDS             7675..7890
                     /gene="atpE"
                     /locus_tag="IV228_001968"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8717849.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7981..8505
                     /gene="atpF"
                     /locus_tag="IV228_001969"
     CDS             7981..8505
                     /gene="atpF"
                     /locus_tag="IV228_001969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8717850.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8492..9034
                     /locus_tag="IV228_001970"
     CDS             8492..9034
                     /locus_tag="IV228_001970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8717851.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9057..10613
                     /locus_tag="IV228_001971"
     CDS             9057..10613
                     /locus_tag="IV228_001971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8717852.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10627..11529
                     /locus_tag="IV228_001972"
     CDS             10627..11529
                     /locus_tag="IV228_001972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8717853.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11552..12958
                     /gene="atpD"
                     /locus_tag="IV228_001973"
     CDS             11552..12958
                     /gene="atpD"
                     /locus_tag="IV228_001973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8717854.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12972..13394
                     /locus_tag="IV228_001974"
     CDS             12972..13394
                     /locus_tag="IV228_001974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8717855.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4522 a         2447 c         2980 g         3729 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat
     2581 tccatttata tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct
     2641 tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga
     2701 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     2761 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     2821 tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat
     2881 atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg
     2941 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3001 tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt
     3061 tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg
     3121 aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat
     3181 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt
     3241 ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct
     3301 ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt
     3361 tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga
     3421 agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag
     3481 ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag
     3541 atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg
     3601 gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa tggtatcctg
     3661 aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg
     3721 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga
     3781 gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc
     3841 tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     3901 aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa
     3961 accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat
     4021 tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg
     4081 agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4141 ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc
     4201 ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag
     4261 aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag
     4321 tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg
     4381 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt
     4441 cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt
     4501 tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat
     4561 tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt
     4621 agataacggt aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca
     4681 aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg
     4741 agtgattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt
     4801 tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca
     4861 tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     4921 aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt
     4981 ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat
     5041 ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat
     5101 ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa
     5161 tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga
     5221 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5281 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     5341 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     5401 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     5461 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     5521 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     5581 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     5641 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     5701 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     5761 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     5821 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     5881 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     5941 tccaaaagca cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc
     6001 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6061 aaaagggaaa aagccatttt acaaagatgt caaaaagaag aaaaagaaaa aataaaatga
     6121 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6181 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6241 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6301 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     6361 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     6421 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     6481 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     6541 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     6601 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     6661 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     6721 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     6781 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     6841 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     6901 gattggatga acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt
     6961 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7021 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7081 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7141 ttacattgtt tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct
     7201 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7261 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7321 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     7381 aagaatttac aaatttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     7441 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     7501 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     7561 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     7621 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     7681 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     7741 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     7801 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     7861 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     7921 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     7981 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8041 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8101 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8161 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8221 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8281 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     8341 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     8401 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     8461 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     8521 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     8581 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     8641 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     8701 ttatgatggg atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     8761 ccttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     8821 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     8881 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     8941 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9001 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9061 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9121 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9181 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9241 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9301 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
     9361 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
     9421 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
     9481 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
     9541 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata
     9601 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     9661 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     9721 tcgttgtgaa tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg
     9781 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
     9841 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag
     9901 gtcgtgaagc ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag
     9961 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
    10021 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
    10081 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
    10141 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10201 tacgcctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
    10261 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10321 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
    10381 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    10441 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
    10501 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    10561 aaggaagtaa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    10621 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    10681 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    10741 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    10801 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    10861 atgctgctca gccgtgctgt gaagaaaact ggttatatcg tcatcactgc agatggcggt
    10921 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
    10981 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11041 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
    11101 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt tccaaaacga agtattcgat
    11161 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11221 aaaatgctgc caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat
    11281 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    11341 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    11401 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac
    11461 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    11521 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    11581 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    11641 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    11701 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
    11761 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    11821 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    11881 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    11941 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12001 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12061 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12121 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12181 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12241 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12301 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    12361 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattacaag aacgtatcac
    12421 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    12481 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    12541 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    12601 tttagcacct gaaatcgtgg gggaagaaca ttataaagta gcaacagaag tccaacacgt
    12661 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    12721 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    12781 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    12841 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    12901 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    12961 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13021 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13081 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13141 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13201 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13261 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13321 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    13381 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    13441 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaaa tgcttctcaa
    13501 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag
    13561 aggttgtaaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    13621 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttattt
//
LOCUS       DACDHL010000063        13626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDHL010000063 DACDHL010000000
VERSION     DACDHL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.063"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            69..770
                     /locus_tag="IV228_001975"
     CDS             69..770
                     /locus_tag="IV228_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8717856.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1342..2358)
                     /gene="tsaD"
                     /locus_tag="IV228_001976"
     CDS             complement(1342..2358)
                     /gene="tsaD"
                     /locus_tag="IV228_001976"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8717857.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2355..2819)
                     /gene="rimI"
                     /locus_tag="IV228_001977"
     CDS             complement(2355..2819)
                     /gene="rimI"
                     /locus_tag="IV228_001977"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8717858.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2826..3368)
                     /gene="rimI"
                     /locus_tag="IV228_001978"
     CDS             complement(2826..3368)
                     /gene="rimI"
                     /locus_tag="IV228_001978"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8717859.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3352..4176)
                     /gene="tsaB"
                     /locus_tag="IV228_001979"
     CDS             complement(3352..4176)
                     /gene="tsaB"
                     /locus_tag="IV228_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8717860.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4265..5245)
                     /locus_tag="IV228_001980"
     CDS             complement(4265..5245)
                     /locus_tag="IV228_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8717861.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5269..6753)
                     /gene="galT"
                     /locus_tag="IV228_001981"
     CDS             complement(5269..6753)
                     /gene="galT"
                     /locus_tag="IV228_001981"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8717862.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6765..7754)
                     /gene="galE"
                     /locus_tag="IV228_001982"
     CDS             complement(6765..7754)
                     /gene="galE"
                     /locus_tag="IV228_001982"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8717863.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8003..8170
                     /locus_tag="IV228_001983"
     CDS             8003..8170
                     /locus_tag="IV228_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8717864.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8232..10043)
                     /locus_tag="IV228_001984"
     CDS             complement(8232..10043)
                     /locus_tag="IV228_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8717865.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10040..10405)
                     /locus_tag="IV228_001985"
     CDS             complement(10040..10405)
                     /locus_tag="IV228_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8717866.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10568..10963)
                     /locus_tag="IV228_001986"
     CDS             complement(10568..10963)
                     /locus_tag="IV228_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8717867.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10981..11943)
                     /locus_tag="IV228_001987"
     CDS             complement(10981..11943)
                     /locus_tag="IV228_001987"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8717868.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11943..12155)
                     /locus_tag="IV228_001988"
     CDS             complement(11943..12155)
                     /locus_tag="IV228_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8717869.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12176..12874)
                     /locus_tag="IV228_001989"
     CDS             complement(12176..12874)
                     /locus_tag="IV228_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8717870.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12894..13436)
                     /locus_tag="IV228_001990"
     CDS             complement(12894..13436)
                     /locus_tag="IV228_001990"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8717871.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
BASE COUNT         3922 a         2953 c         2337 g         4414 t
ORIGIN      
        1 taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg
       61 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
      121 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      181 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      241 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      301 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      361 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      421 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      481 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      541 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      601 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      661 aaactgctgg acaagtaatc aaacaactac gtgattccgg actgttagat tatgacaaaa
      721 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      781 aataagcagt gaccctgata tttatcaggg tcactgctta ttttcctccc taaaacttct
      841 gaaagatccg gcagttttag atcactgggc aagactcaaa gattttctat gtgtgtttca
      901 gttatgtaat attcactgtt tgcaaaaaat actgatagat taactagagg aagcgtcttg
      961 tcctcatccg acctagatta ttcgatattc ctcgacaata aacgattaat cagtgagggc
     1021 aatcagagga acggataatg atgaataagc tgtaaaaaat agggctttga caaaagcgtg
     1081 tcacagccct tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata
     1141 atgttcataa acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg
     1201 acaggcttac gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa
     1261 cagccaggtc aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag
     1321 ggcagatttt tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg
     1381 atagctagca aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc
     1441 attatctcca caaagtcgaa gagggggaat cactagttct acctcaggga gtttagttgc
     1501 caaagcatcc tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctacaat
     1561 cagttgcttg actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat
     1621 cacacttgct tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg
     1681 tgcattatgc accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt
     1741 atcttcatgg atcatcgctc gtggaaaatg gtaattatct tttccctgat gagctagctg
     1801 atcgatttct tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc
     1861 ttctcctgct gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat
     1921 atagactaat tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg
     1981 tttaaccaga cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa
     2041 ttggtgtgcc caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag
     2101 tcctggtcca taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac
     2161 cagtgcatct tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg
     2221 aactacgcca ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat
     2281 ctctgttcca tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag
     2341 aatcaattct tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca
     2401 tcttccgtcg gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat
     2461 tcataaagtt ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa
     2521 tcttttattt gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt
     2581 tctgaaactg caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca
     2641 ataaagccac agatctggtt atcttcaact aaaagcagat aatcagagtg aggctgttgg
     2701 atgtctgcca aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta
     2761 atcatccata cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac
     2821 taatctcact cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg
     2881 taatatcttt ttttgatcgt tgttgcttga aaacctaact tccgataaag tctttgtgca
     2941 tcttgattac tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag
     3001 ttctcgattt cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg
     3061 gcaatattcg tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt
     3121 cttccattat caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata
     3181 aaggcacttt ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc
     3241 tctttgatat cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgatt
     3301 gttttttctg tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt
     3361 ttcaacataa ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac
     3421 ccgttttaaa tagcgaggaa gaaaagcttg tttctctgca actggttcag ctaaagcacc
     3481 taattgagcc acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc
     3541 actaggcaat atttccttta tttcatagaa gaatttgtga caatcactcc cgacgaaata
     3601 aatagcttca ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc
     3661 ttcaagtaca atttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt
     3721 tcttcgagca tcaaacatgg gcacaataat tttatccaca ccgatgcaat tagccgcgat
     3781 ggttttcaga ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc
     3841 tgtcgttaca cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg
     3901 gtcaatgtct tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag
     3961 tgttacactg tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc
     4021 taccacaccg atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt
     4081 gatcctttct gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat
     4141 gaaaatacca aaggcaaaac tagtacaatg atacatgatt atctgctggc gggcaaattt
     4201 tgccccttcc tgaaacacaa aaaagctgcg gtatcacttc atttccccac agcttttcca
     4261 tcttttattt cgttgattct cgaataatca actctgtttc cacaataatc tttcttgaaa
     4321 ccggtgcttt ggaattactt aattgaagaa tcgtctccac tgctagctct cccatccatt
     4381 cggtataaac ttttacagta gaaagagagg gcgttacata tttcgctaca ctgacatcat
     4441 tgaaacccat cacagaaata tcttctggta ccactaaacc tttttcttga atcgctttca
     4501 gtgcgccaat cgctaatgca tcattagctg caaaaagcgc atcaggaaca tgcggattcg
     4561 tttttaaata ctgcttcact gcttgatatc ctgactcgac agaaaaatct gcttcgataa
     4621 ttggaccttc gtcgtagcct ttttctgcca aaacttgttg aaatgccata attctgggat
     4681 cttttagccg ctgatggttt ttcttcgtat attctgttcc agcaagcata gcgatatgtt
     4741 gcgctttatg ggagacaaaa tgttcaatga ctttttgaac acttgactta aagtcgacaa
     4801 ctagagagtc gattccttga gaagtgccgt cagaatctac caaaagcaag gcattttggg
     4861 atcctttcat ttctaataac tgagcttcgt cgaattttcc tagtgcgatc gttccatctg
     4921 tcggaagatc actaatggaa tcccacgttt ctttcaataa agtaacgttt gcttcttctg
     4981 cctttttttc gattcctaga cgaatagata aataataaag atcttcaagc tcttccgtat
     5041 cgtcgtacca ctggatcagg cgaagcgttt gcttagtatt tgctgcattt ttacgatgtt
     5101 tcgtataatt taagcgttct gctgcttcaa aaattttttg ctttgtggct aagccaacgg
     5161 aaagatcagg atcatagttg agaactcttg aaacagtagc aggagaaact cctgccaact
     5221 gcgcaatgtc tttaattgtt gccatttgtc tctcctcctt ttttacgttt ataaaattgc
     5281 agtaaatcgt tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc cagcgtcttc
     5341 cagtacacgt gcaaaaacct gacctacaga tttttgtacg acatcggtga ccgtatcttt
     5401 cgtcaaatca ggatgttctt tttttaattg ttctgcccac ggacgatgat attcagccat
     5461 ctggttttct tctcctaata agaaatgctc tacttctact agttctggtt tcaaacgagg
     5521 tggtaaaaca gcaagcccca ttacttcgat caacccaatg ttctcctttt tgatatgttg
     5581 gacatcttta tgtggatgaa aaatcccatc aggatgttct tctgaaacat tgttgtctcg
     5641 aaggacaagg tccaactcaa agtaattgtc ccttcttcgt gcaattggcg taatcgtatg
     5701 gtgtggtgta ccatctgctg aaaaagctct gatagatact gtttcatccg aatatgcttt
     5761 ccacttttcc aatactgagt tagctgcttc aatcaacaat tttttctctg ttgattgcaa
     5821 acggatcaca gacatcggcc atttcacgac tcctgcgatg acttgcggat aatctgctag
     5881 tttgataagt ttttctatcg gtgcttttgc cataggaaat tcatgacgtc ctgcctgata
     5941 atgatcatga gaaagaatcg atcctccaac aatcggcaag tcagcattcg aaccaacaaa
     6001 gtaatgaggc aagacttcag taatccgaag cagtctagca aatgtttcct ttgtgatttt
     6061 catcgggcgg tgttgttctg ataaaatgat cgaatgttca ttgtaataag cataaggaga
     6121 atattggaat ccccagcttt caccgtctag attcattcga ataatccggt gattggtgcg
     6181 ggctggatag ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca tgcaggtagg
     6241 atagtctact tttaccgcct cgcgttctgc tgcgatttgt tttggatctt tttctggctt
     6301 agataaattg attgtgattt ccagttcacc atactccgac tctgccggaa atttgatgtt
     6361 ttttgcgatg gctcttgttt tgatgtagtc atttttctta cttaaattaa agaaatactc
     6421 agttgcttcc tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga cgactgaagg
     6481 tggcggtgtg agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg ctggcaattc
     6541 tgaaagaaca ccattttctc ttgccttagc gacaagctga tccaataagt ctacagattc
     6601 ttccttaact ggtcttactt caatgctttc tagagcatct tctcctacca tccctaaaag
     6661 acgattctgt aaatataagc ggtccatttc catccatccg ccagcttcga tcgcaagtgt
     6721 ggcaaaatca caaatcgttt gactgattga catatctcat tcccctattc ttcataacca
     6781 tttggatggc tgacatgcca atcccatgct gttttgataa ttgctttgat atctgtgtat
     6841 tcaggtttcc aacccagaac ctctctcgct ttttcacttg aagcgactaa tcggctagga
     6901 tctccagctc ttcttggtgc tatttctgct ggaatttctt ttcctgtcac ttctctagct
     6961 gcttctagca tttccttgac tgagtatcct ttgctgctgc ctaaattgaa gaagttgctt
     7021 tcatttcctt ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa atcttctact
     7081 tgaacataat ctctgataca tgttccatct ggtgtatcat aatcgtctcc ataaacagcc
     7141 aatgcttttc tttgaccaag agctacttgt aaaatgattg ggacaagatg cgtttctggt
     7201 gtatgatctt ctccgataga tgcatctgct tttgctccgg ctacattgaa gtagcgcaaa
     7261 gcgacatagc gcatgccata tgcttggtca caccacttca tcattttttc catcatcaat
     7321 ttactttcac catacggatt tttaggatta gctggtgtat cttctgtgat tggagactct
     7381 tttggttctc cgtaagttgc tgcagtagaa gagaacacga ttttattcac attgaactcg
     7441 tgcatgactt caagtaagat ttgcataccg tatacgttgt tgttgaaata catcaatggt
     7501 ttttcgacag attctcctac taaagagctt gcagcaaaat ggattactcc ttcaatcggt
     7561 tccttttcaa atacggaacg tagaaattct ttatcccgga tgtctccttc ataaaaatga
     7621 gcatctggat gtactgcttg tttgtgacct gtgagcaaat tatctaccac agctacttga
     7681 tacccctttt gaactagctg gtctacagcg tgcgaaccga tataaccggc tccgcctagt
     7741 actaaaattg tcataaaaaa cactcctttg attagtaaaa ttgtgtttgt tgattatata
     7801 taatttaaaa aaacgaagtt tacttttatg gtgaaaacaa acaaagtaaa atttcgtaaa
     7861 ttcatatggt aaatttataa taaaacattt agtaaacatt ttcaaggatt ttttgcaaag
     7921 gtttacaaac aaacgtaaaa ctagcctttt tttctctttt gttccataat agatataaca
     7981 aataataaat gaggtggatt caatgaaaaa atttgtatct ggaatattag taggaagtct
     8041 ggcaacagcc gctgccgttg ctggtttggt cgcttctgtg aaaaaaacag tgatcgatcc
     8101 aattgacgaa aaagaagcaa tgatcgaaga aaaccgtaaa aaagcaatgc gcaaacgcgt
     8161 ttctcgttaa taattattta aaaacacgca tgaagacttg tgaaagaaca gataagtcat
     8221 catgcgtatt tttatttgtc agtcaatata agttgtgcta ttttatctgc ctgtctcaga
     8281 ttccatggat ctgtataagg tctagctact gctgcagtta gccaatccac accttcaaca
     8341 ggtattccat agcaataaaa gtcagactgg atttcccctg aagcatcaat cacgcgattc
     8401 gtacctcgct caacatctat agcttctgaa tgataacttc ctgtattatc tgaaaatgaa
     8461 aaagggcgaa caagtttgtt ttccttcaaa ttttttatga caggattact tgaacgagaa
     8521 aagcttgttt gtggcaatcg agcttcaatc aaatagcgac tgctgatttc tttatcttgt
     8581 agagaaataa catagcggtt gtgcttttgc tgaatatcaa atggcggttc gaacaaatga
     8641 aaaatacccg catctatcag tgctgctaat tctttcgtcc gaatgactgg cggaccgatc
     8701 gttaaaaagc tatttagatg agtaaaccat ccccatagta tttctttgta ttcctttgca
     8761 gaaaaaacgt gccattcaat agcctgccgg attggatcac gccagtcttt taatgcatca
     8821 aatgttgaag caaatgctcc tgtgcaattg cctttttccg cttcttttat ctgcccttca
     8881 ataaatgctc ggctagcttc tgcaaaggac tcatcctgat aggtaagagg aatttctaaa
     8941 tacgaccaat cccatctata agggattagt gctggatgct ttccgagaca ctcctctgaa
     9001 gagagggaga gaaaatcatg ctcaaaattt ttctttggaa tcggcaaatg atattcttcg
     9061 atgattttct tgtaataaaa caactcaacg tcttttttca gatagtcaaa aaatgtttcg
     9121 ccagtcaaaa atcccgactg atgccattgc tccaggtttt cttttgttag taaccgtggc
     9181 catgccattg atccgccttg cttttggttt cttcctcgag ggtaataagg gagccccctt
     9241 cttgatccac tgtatacaat cggttccttg cctgatggat gatagacaag ttttcctttt
     9301 tcgttctcga agcgcccttc tcttgatact gtaaacaatc cgacataatc aaaaaaactt
     9361 aagcctagac ctcttaaaaa gacattttct ttaggcggta tcttcgaaag atcgacatct
     9421 gcaggattag aaggaagctg atacagtaaa tcatttttct ttgcgtagtc tacccacact
     9481 ctttcttctt ctgtctcatc attttcccag tgaccgcttg ctaaaacaag ctgatcgata
     9541 gaatagcttt gctgatctgt ctgtaaaaga taaccatctt ttcttttgct gacttttttt
     9601 actaaagaat aaacaaactg aatagaagct ggaaattctt cttgcaactt tgagaaaaac
     9661 cagcgttgat agagtccgta taaacatcga gtagattgct gatctttttc cagacgattg
     9721 atttcttcta taattagttg cttatttttg gttaccgtct gttcgatata atctgtagca
     9781 ggtcctttac tccattgaaa caagttgggt ccctgtgtta ccaatcctcc actggacaat
     9841 gtttcatctg taaaaagcgt cacttgttga gaaacagaat tcattaataa ctccggtggc
     9901 tgattcaacc gccaaactcg acctccagga ccattgggat cgaaaagaaa aagttcaaga
     9961 gaatgtctgt cctgattatt atggagaatc ctttctgcta tacttaatcc tctaggtccg
    10021 gcaccaatga tccctatttt catttgcatg cctccatttc gataaatttt ttcaataaaa
    10081 cagcatggtt ctgatgttca ttttttgcac cataaacaaa agtaatgact ggatgttctt
    10141 tcataagttg actgagttta aaaatagctt gcttcttttc ttcttcgaaa atcaattctt
    10201 gctggtattt ctttgcaaat gaagcaaatc gttctggtac atggtcaaaa gttttgcgtg
    10261 cttcattact aggtgcaatt tctttcaacc acagatctaa ctgcgctttt tcttttgaaa
    10321 ttcctcgtgg ccagaaccta tccacgagta ttctatagcc atcctctttt tcagcatcaa
    10381 ggtaaacacg ttttaatttg atcatagttg tttccttctt tctgcctttt cgcttagctt
    10441 atatatgtag ataatagcac aaaataaaaa aaatgaacgg cacaactatc cattgcgccg
    10501 ttcatgtgat cttggtcaaa atctaatgat tgattcggtt tttaaagctg tcatacaatc
    10561 atctgtttta tactttttta cttttgattt ttccagacca tccgtcatat ccacccttaa
    10621 gtaaataaat ctcttgataa cctgctttac gtaatttatt tgctgtacgg atgctcaagc
    10681 ttttcttttg atcgtaaata tagacaggct ggtcttttcg aatagatcct aatgaatctt
    10741 tcaaaacagt gtaagggatg tttcttgcac ctaaaatatg cccagcatca aacacatctt
    10801 tttcgcgaac atcgattact tgtgcttttc tcattccttc tcgaaattct tcttctgtca
    10861 aagtcgttgc tgaacgcttg gccattactc gtagatagac ttcccaaaag atgattgcaa
    10921 gcacaatcaa tagcaaaatg atattaatta cccataaaac catgtaatgc ctccgttttt
    10981 ttaagcaaat ttcaaggcta atgaggcagc accaatgaca ccagcttcat taccaagctc
    11041 tgctaatttg atttttgttg attctcttac ttgcgggaaa gtaaattctt taaaatattt
    11101 ctctacacgg ctacgtaaaa attctcctgc tgccgatact cctccaccta acacaatact
    11161 tgacggattc aatgtattcc ccaagttggc gcaagctaaa cctaagtaga agcatacgcg
    11221 atcaacaacc atcaatgcaa acggatcgtc ttcttgagcc atttcaaaga tatctttact
    11281 tgttacttct tcaccgttgt ctaacattgc tttcagtttt gaatctcctg cgtattcttc
    11341 tgccaagtaa cgacctagac gtaccacacc agtagcgcta gacacagttt ctaaacatcc
    11401 gcgtttaccg caagtacatt cgaacccttc aggatccaca gtgatatggc cgatctctcc
    11461 gcctgctccg ctcacgccgt gaatcaaatt tccgccagca acgataccac cgccaacacc
    11521 tgttcctaga gtaacgaata caacgtctgg atcgttttct ccagcacctt tccatcgttc
    11581 acctaaagca gcaacattcg catcattatc gatagcgaat ttgattcctg ttcctgcctc
    11641 gatcagtttt ttcacaaatt gaacttctgt ccagtttaag ttatacgcac caataaccgt
    11701 tccagcttcg ctgtccacgc ttcctggggt acccatgccg attccgataa agtcttcggc
    11761 tttcatatta tataaattca aacgatggtt gattgattca atgatttcag gaacgatatg
    11821 cttaccatca tccaaaatat tggtatcgat gctccatttt tgttgaatct ctccttctgg
    11881 tgttaaaatt gcaaattttg ctgttgttcc acctaaatca atcccaataa tcttcttgtc
    11941 cattacaaat ttcctttctt tcggctttct tcaattcggt gctctctttt taatatgcgc
    12001 cacgctgtca tgtatgtatc atgatcaatc aaacggctat catacagatt acgcaattcg
    12061 atagtcatta attcaatgtc atagatgcgt gcacccacat agataaagat gccaaatttt
    12121 ttgaacagct gctgtacatc atataagctc tccatcttta agcacttcct tcatattata
    12181 caagtaaacc atattttttt aaacccagta aaatacatat tacaaaaaga aatacaaaaa
    12241 ctagtgtaga aagaatcctt tggtgtatcg aatagcttgt ctgttgtttc ggtagaccca
    12301 aaacattccc taacactaat ccgccaatta gcccaccgac atgtccccaa acatctacag
    12361 aggtatccag aagaccaaaa acaaatgtca acacaataaa taaaagaaaa tgtctgacta
    12421 gctgctggat cgctgggtta tgtttaaaat gaaaccctaa gataaacaac gcaccgaaca
    12481 aaccgaatat cgaagtactc gcaccaccag acagaacatt cgcttcgtta aaagcaaagc
    12541 ttgctgtatt tcccattatc ccgctgaaca tataaatcaa gaaaaaacgc caatggccat
    12601 agattgcctc gatttgctgc cccataaaat acagtatcag tgagttcatt acgaaatgca
    12661 tcagtccaaa atggatgaag attggtgtca ctaaacgcca ccattcattg aagtgaacaa
    12721 gataaggacc atacattccg gcataataag ggatctcaag tcgcggaaat aattccatca
    12781 tgaggaagac cgctgtctgt atccctaaaa aagcataggt ccataacggt cgtcttaacc
    12841 atgcacgcaa ttttatttgc tgttgataat tcatgaaaac ctcccatttc attttagtca
    12901 ataaacaatt gttggatctt ctgatcaaag ctctccggtt gccattgttc attcaattgt
    12961 tcacgaaaaa caaggctaca tgttttttta tgaaaattat ttaaataccg gtcgtaataa
    13021 ccaccgccaa aaccgattcg ataaccggct ctattgtaga taatacctgg tacaatgagg
    13081 agatcgatca tctccggcaa atagagcttc tctgattgtg gctcaagaac accaaaactc
    13141 gaacgtacaa gaacggactg ttcatccatt tcataaaacg ccatctgacg tttcggcaaa
    13201 gttttaggta caactatttt cttttgttct ttttctgctc gttggataac cggctgtgta
    13261 tccagttcaa attcattcga taaagtcgtc cctatcactt ttgctgactt ccagtactcg
    13321 ctggcgaata gctgtttata gattcgctgt tctttttcct ttttcttgcc acttttggcc
    13381 agatttttca gcttttcgat tcctttccgg cgtaatctta ctttcaattg gtccaaaaac
    13441 tcacccttcc ttctctcata aaatggtata ggaaaaaaag aaaaatagca atcgaacttt
    13501 caaataaatg cataaataga ataaaaagaa gacctagaaa tgattggcct tcctttttta
    13561 ataaatttca actgttcagc gcgtgttttt gtctgataaa aagtgcatac agaaaatatg
    13621 gcgcac
//
LOCUS       DACDHL010000064        13532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDHL010000064 DACDHL010000000
VERSION     DACDHL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.064"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..207)
                     /locus_tag="IV228_001991"
     CDS             complement(<1..207)
                     /locus_tag="IV228_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8717872.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEE"
     gene            complement(207..437)
                     /locus_tag="IV228_001992"
     CDS             complement(207..437)
                     /locus_tag="IV228_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8717873.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            724..1062
                     /locus_tag="IV228_001993"
     CDS             724..1062
                     /locus_tag="IV228_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717874.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1267..1506)
                     /locus_tag="IV228_001994"
     CDS             complement(1267..1506)
                     /locus_tag="IV228_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8717875.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1482..1829)
                     /locus_tag="IV228_001995"
     CDS             complement(1482..1829)
                     /locus_tag="IV228_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8717876.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2561..3142)
                     /locus_tag="IV228_001996"
     CDS             complement(2561..3142)
                     /locus_tag="IV228_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8717877.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3325..3951)
                     /locus_tag="IV228_001997"
     CDS             complement(3325..3951)
                     /locus_tag="IV228_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8717878.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3973..5304)
                     /locus_tag="IV228_001998"
     CDS             complement(3973..5304)
                     /locus_tag="IV228_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8717879.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5459..5860)
                     /locus_tag="IV228_001999"
     CDS             complement(5459..5860)
                     /locus_tag="IV228_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8717880.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5946..6152)
                     /locus_tag="IV228_002000"
     CDS             complement(5946..6152)
                     /locus_tag="IV228_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717881.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6247..7749)
                     /locus_tag="IV228_002001"
     CDS             complement(6247..7749)
                     /locus_tag="IV228_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8717882.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7767..8222)
                     /gene="argR"
                     /locus_tag="IV228_002002"
     CDS             complement(7767..8222)
                     /gene="argR"
                     /locus_tag="IV228_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8717883.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8320..8937)
                     /locus_tag="IV228_002003"
     CDS             complement(8320..8937)
                     /locus_tag="IV228_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717884.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9158..10531
                     /locus_tag="IV228_002004"
     CDS             9158..10531
                     /locus_tag="IV228_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8717885.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10606..11718
                     /locus_tag="IV228_002005"
     CDS             10606..11718
                     /locus_tag="IV228_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8717886.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11790..13427)
                     /locus_tag="IV228_002006"
     CDS             complement(11790..13427)
                     /locus_tag="IV228_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8717887.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         3682 a         2858 c         2386 g         4606 t
ORIGIN      
        1 ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat
       61 gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct
      121 tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt
      181 aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct
      241 tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt
      301 tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct
      361 gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta
      421 gattttgtaa caagcattat catcactccc ttaataaaag tatactatat aacactaagt
      481 aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag tatatcttat
      541 tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa aaaaataatc
      601 caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta accccccgct
      661 accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac aggccatcct
      721 atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg
      781 ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg
      841 cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa
      901 cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat
      961 gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg
     1021 ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt
     1081 ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg
     1141 tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc tgttctcttt
     1201 gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg
     1261 attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc
     1321 aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg
     1381 gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat
     1441 tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga
     1501 aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg attttgcaaa
     1561 aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt aatcatcaat
     1621 tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag gatgttttct
     1681 tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc tgctaattct
     1741 gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag gagctctgac
     1801 tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt tttctatcat
     1861 acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga actaaaaaag
     1921 ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc tcggtcacaa
     1981 gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg tgactcggag
     2041 tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt tctcggtatt
     2101 aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct taatactcga
     2161 aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg
     2221 taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg ttcttattac
     2281 ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctaacctt
     2341 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat
     2401 actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga agtagcttct
     2461 tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
     2521 atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag
     2581 ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga tcctgtgtga
     2641 ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct tgacggtctg
     2701 cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc gtatacttga
     2761 cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact gtactcaact
     2821 caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca tgatcagcga
     2881 aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca tttaagatgt
     2941 cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct agacaaactt
     3001 ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa gctgctagaa
     3061 tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg gtcgcttcca
     3121 agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag tcctatatta
     3181 tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc caaattatct
     3241 gacacagccc tcactataaa actgttctca tatagctaga tgttcattga ttcaagaact
     3301 gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct ctgttgtcat
     3361 cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt cttggccagg
     3421 agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca attcacgcat
     3481 atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt cgattccatt
     3541 ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat gaggagagct
     3601 gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg ctcctgaacc
     3661 ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa gcatcatatg
     3721 ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat gaggagaaat
     3781 aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat tttcgatcgt
     3841 attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa
     3901 ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca tggtggattc
     3961 tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg gatgagaata
     4021 gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg aaatcaagtt
     4081 gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt cagtttcagg
     4141 gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt tagggacacc
     4201 gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt gtttttctgt
     4261 taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag ctttccggtt
     4321 attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat aatcatctag
     4381 agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa tgttgtctgc
     4441 atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt taacatctgt
     4501 attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca aaacttttga
     4561 cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg ttgattcttg
     4621 gtctgctact tgatctacag atacacctag atggaattcg attcccgcat ttgtcatgtc
     4681 ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag aatgggcatc
     4741 aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca agccaatata
     4801 tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg cttgcgtaga
     4861 atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg gttcagaacc
     4921 agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat tcgtcacttc
     4981 gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag ttgcctcatc
     5041 gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt tccgagcaac
     5101 agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag aaggaataca
     5161 gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga ctaaaaccga
     5221 ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa aaccgatcac
     5281 aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac cgattggtac
     5341 aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa
     5401 aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct ttattcttct
     5461 aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa gggcagaatt
     5521 catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg atatgcactt
     5581 ctaatactac accgatctga tagccgccat catctaaatt ataaagagag acacgtgctt
     5641 ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc gccccattga
     5701 aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct gcatggattc
     5761 ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct ccattgcgcc
     5821 cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc ctttcgattg
     5881 accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt ctacaaaaat
     5941 tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa caaaaaaata
     6001 ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt ttctacataa
     6061 tcttttatat gattgatttt aagatacata tataccagaa agttgacccc ataaaccaat
     6121 actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg gtctccctac
     6181 cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc tttcaaaaag
     6241 actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata attttgctaa
     6301 ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta atactagtaa
     6361 cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct cttctagatc
     6421 atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt cttccaacag
     6481 tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt
     6541 taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt catctaatgt
     6601 gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga catatgcacc
     6661 atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga tggctagctg
     6721 tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc gatagccgga
     6781 aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat gagaagaagt
     6841 aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg tacttaatac
     6901 tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg cagaacgatc
     6961 cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag cctgcttgat
     7021 actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc ggccatctgg
     7081 ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt tttcagcaaa
     7141 gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta gtggtttgct
     7201 ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa
     7261 aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct gctgtgatag
     7321 gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt ttattccatt
     7381 ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg agaactgttc
     7441 aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga tgacatgtat
     7501 cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc ttatcctact
     7561 gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct tctttttctt
     7621 tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca agtttacttc
     7681 atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca gcgctttcat
     7741 atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt ttctgcaaat
     7801 cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc ggccatcgtt
     7861 ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc ggcaccattt
     7921 cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac ctcgcgtcta
     7981 attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa taatgtatag
     8041 cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat gtctcgagaa
     8101 atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt
     8161 gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg tttctttttc
     8221 atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc ttcatatttt
     8281 ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt ctattttaac
     8341 acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga tgtaaatctc
     8401 tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt ctaatttctc
     8461 aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac aaagaccttc
     8521 ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc tagaacgctg
     8581 aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca tcaattgata
     8641 agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca gtccccattg
     8701 tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat tttttttagg
     8761 cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt taaagtcttc
     8821 ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa cctctagtat
     8881 ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt tttccatttt
     8941 tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg agtgacttgg
     9001 ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa ataaatcgta
     9061 cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata atagaaatca
     9121 ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa aacagctgct
     9181 tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat tgatcgagac
     9241 ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga ctgcacaagt
     9301 ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc tgtctactta
     9361 tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc ttttcttggc
     9421 aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg gtcgattgct
     9481 ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct tgacatttgc
     9541 ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg ccattgctcc
     9601 agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga atcatatctt
     9661 cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg ctattcccga
     9721 gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa tttgtctttt
     9781 gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct ggattgcttt
     9841 aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag tggagcatcc
     9901 actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga ttggcttctt
     9961 agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt
    10021 tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag cggttggtgc
    10081 tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga agccaatttt
    10141 tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca taggacacgc
    10201 ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg gattatctta
    10261 cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag gagacgggaa
    10321 cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat tagtagcggt
    10381 cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta ctacattagg
    10441 atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg ctcgattcgc
    10501 ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg ttgcatctgc
    10561 cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga aaaaattgcc
    10621 attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac atcaaaattc
    10681 aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc tgggattatc
    10741 tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa agacggcgct
    10801 gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta cgcacaatca
    10861 gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct tgccgagaaa
    10921 cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga acagacatct
    10981 caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg tggtcgtctg
    11041 gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca aatggagcag
    11101 atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa gggaccgttt
    11161 ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa gcctttatta
    11221 caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt agcatttcag
    11281 acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga agctgatttc
    11341 attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga tcaaggctgg
    11401 gatttggctc ctactagcga agcttataag ggattgacta agagtacaga acagttcttg
    11461 actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac ccgtgcttat
    11521 acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt actggctgct
    11581 agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct attagccaac
    11641 ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc cacctatatt
    11701 acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc gatattgcgc
    11761 atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa attctccaaa
    11821 ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat gaatcgtaaa
    11881 cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa agaaacagga
    11941 aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt gaattctgcc
    12001 gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat ttttttatca
    12061 tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa caactctcga
    12121 aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa tggttcaatg
    12181 actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg ttttgttgct
    12241 aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat tggcggaagc
    12301 gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg atcgactcgg
    12361 tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat atctcctgtt
    12421 actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca agtaaggaaa
    12481 gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta agaaggttcc
    12541 aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg caagatcgaa
    12601 acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga accggacaac
    12661 ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat cccaccgtgg
    12721 caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt
    12781 aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc atcaaacaaa
    12841 atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat ataaagatct
    12901 ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt cgctggctga
    12961 aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag caaatgactt
    13021 tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa ctggcgcttg
    13081 cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag
    13141 tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc attgaatact
    13201 agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt taaatctgtg
    13261 atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc aaaaacttca
    13321 tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga taacagtgga
    13381 tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc tcctcctcat
    13441 tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat ttgtactgct
    13501 gcgctgtggg aagcaaacga gttatttttc ac
//
LOCUS       DACDHL010000065        13190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDHL010000065 DACDHL010000000
VERSION     DACDHL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.065"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            10..2382
                     /locus_tag="IV228_002007"
     CDS             10..2382
                     /locus_tag="IV228_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717888.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2443..3903
                     /locus_tag="IV228_002008"
     CDS             2443..3903
                     /locus_tag="IV228_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8717889.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3914..6118
                     /gene="topB"
                     /locus_tag="IV228_002009"
     CDS             3914..6118
                     /gene="topB"
                     /locus_tag="IV228_002009"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8717890.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6358..6951
                     /locus_tag="IV228_002010"
     CDS             6358..6951
                     /locus_tag="IV228_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8717891.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6964..7863
                     /locus_tag="IV228_002011"
     CDS             6964..7863
                     /locus_tag="IV228_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8717892.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7866..8363
                     /gene="ssb"
                     /locus_tag="IV228_002012"
     CDS             7866..8363
                     /gene="ssb"
                     /locus_tag="IV228_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8717893.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8729..8989
                     /locus_tag="IV228_002013"
     CDS             8729..8989
                     /locus_tag="IV228_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717894.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9434..9640
                     /locus_tag="IV228_002014"
     CDS             9434..9640
                     /locus_tag="IV228_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8717895.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9688..9891
                     /locus_tag="IV228_002015"
     CDS             9688..9891
                     /locus_tag="IV228_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717896.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9907..10344
                     /locus_tag="IV228_002016"
     CDS             9907..10344
                     /locus_tag="IV228_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717897.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10337..11044
                     /locus_tag="IV228_002017"
     CDS             10337..11044
                     /locus_tag="IV228_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717898.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11424..11603
                     /locus_tag="IV228_002018"
     CDS             11424..11603
                     /locus_tag="IV228_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717899.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11928..12191
                     /locus_tag="IV228_002019"
     CDS             11928..12191
                     /locus_tag="IV228_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8717900.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12185..12535
                     /locus_tag="IV228_002020"
     CDS             12185..12535
                     /locus_tag="IV228_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8717901.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12559..13173)
                     /locus_tag="IV228_002021"
     CDS             complement(12559..13173)
                     /locus_tag="IV228_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8717902.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4880 a         2178 c         2718 g         3414 t
ORIGIN      
        1 ggaaagaaca tggcagaaga aaaagaacca ctagacaata gtcgtttggg aaaatccaaa
       61 agaaaactag tacgattgca aaatgaatta aacgaacaaa ttgaaaagat gtttgaacac
      121 caaaaaatga cgaacggtca accaatgaac gacaagagga atggccgttc atggtttaac
      181 cagcaagaac gaatcgaaaa taaagtccaa agtctgagag aagagatcaa gcaacaagaa
      241 gaatacgtgg aaaagcttga aagacaagaa gagctaaagg aaatgggcta taacaagtac
      301 ggcggactgg atatgacgat cgataatatt ccaagaatca aagaagaaat cgaacgcttt
      361 gaaaaaggtg aatcggcttt ttcagccgcc acgatccgaa aataccaacg gaagttggaa
      421 acattagaac agctgaaaga aaaatcagaa aaaggaaaag aaaatatcct ccctgaagta
      481 caagcgatca ttgacagcgg acgagtgacg caatggaaaa aaaatccaac catttatttt
      541 ttgaaaggct atcgaaaagt tgctttagaa ctgaatgaaa acgggggatt tgaggaatca
      601 aaaaaatacc aagccacgtc ggaagaggaa aaagcagctg ttcgtgaatt acttgaagag
      661 gctagaccaa ctgattctat gaaggagaaa gaaaacgatc atttctatcg agtagaattc
      721 aacgaaaatg atccagaaaa aggattgaaa agttatggtg gggagatcgt aacattcgaa
      781 ttgatcgatc aactcaaaac attggataac caatacgccg cagaagcagg ttattataaa
      841 ttctattttg aggaagtgaa gaacggagaa gtagtcgatc agatccgaat ggatctaggg
      901 gatggcgtca acagtaatca accaatctat ttagagttag caaaattgtg cgaacaagta
      961 cctgagaagg aacaaaaaca atcggtcaaa aatgaacctg tttcgaaaga acaaatgcag
     1021 tcgaaagaaa caaaaacaaa acaaaagggc tatcaaaaac gaacggcaga agagattaaa
     1081 caagaagtta aatccctttc gcaacaggca ctggatgcgg tgaaaaagca tacccattct
     1141 cctgaagacg tcaaagaact tttgaacttt atgtctcgtt ttccagaacg atcctttaga
     1201 aatcaattat tgattgagga acaatttcca ggtgcaacgg cttgtttagg tcgtgccgcg
     1261 ttgaaaaaag aaggaatcta tatcaaaaaa ggggaacaag ggaacaagat ctttgtcaga
     1321 aaaacggtca aagggtttta tgagaaagga cgtggctttg ttcgagaaac agaagctaca
     1381 ccagaagata aaaaacgaat cgaaagtggt caaatcgaag tgatcaaaaa accttactat
     1441 acgattgaaa aagtgtttga tatcacacaa acacagttaa aacctgaaga ctatccgaaa
     1501 atctttccga accgtgtctt tgattttcaa ttggataaaa aaggacagtc agaacttcaa
     1561 gctgggattc aagcggtagc caacaatatt ggaatacaaa ttcgagatat gacagaaagt
     1621 gaagtctatc aaagagaatt aggacaagca cgtggggcgt atgttcgtaa cgaatttact
     1681 ggcgaagaag aaatcgtcat gaatactcga aatactccta cgcagcattt agctacaagt
     1741 attcatgaat tagcccatgc aagaatgcac aaattttcag aattagatac cgcaactaaa
     1801 gaattgcagg cagaaatgac ttcttatatt gtgtgcaaac attttggaat ggacacgtcg
     1861 gagaaagcta ttccttatat tgcagcatgg acgaagaatg gtcagacgtt ggatgataag
     1921 gaagctgcgg aacgtgggaa aatcatgaat gatgtcagcc gtgtcgcaaa tgaattcatt
     1981 caaaccattt ctaatgaaat caatcaatac agagaaagag aaccagagat ccaaccagca
     2041 gaagtgaagc aggagcataa aaatacttta gaaacgccag agaataagaa tattgtgccc
     2101 gttggttcac tttggatcga caagaaagac gggaaagtaa tggaagaaga tacgggtcga
     2161 acgttacatt tgaaggatgc ggcgtttatt gaagatccgc agcataatca agtaatttta
     2221 aaaggaaaaa gttatggaaa agagatccaa acggcgttac cttcagaaga atttgcgcta
     2281 ggaaatccat tccgattttc acttgagaca gtgtcagaag tggcaaaacg aaaaaatgaa
     2341 atctattatg aacaacaatt gtcacaaaaa gaacgtgtat aaaacaagta aaaaagacta
     2401 tctgaaaaac ggatagtctt ttttcttttg ggaggtttta aaatgaccga gaaaaaaatg
     2461 agtcttattg atcgatgtaa acagatcgat atagtggatt ttgctcgaaa caatggaatg
     2521 gctgtagtca ataaaggacg agattatcgc ttagaagacc atgattcatt tgtttttgat
     2581 cgaagaaaac aacgttttta ttggaacagt caaaatatta gtggcgacat tattgaatta
     2641 gctaaacttt ttttcataga caaagagatc caagacccta agcaacaatt taaagcagcc
     2701 actgatttta ttctaaagaa tgaagacaaa acagaacgag tggaaaatct tcattttgaa
     2761 acagaaaagt acaaagatca ccccgttgat tatcaaccac tcacagaaaa aggaaggaat
     2821 tatctgaagg aagaacgaaa actaccagag tggctgattg attatgcaga aaaagaaggg
     2881 ttgattgccg aactaaagcc aaaatatgaa cggcaaaatt tcttagttcg agacgatcgt
     2941 ttagatcatg cggtcgcctt tttatggaaa gatccacaga caaaagagac agtaggggcg
     3001 agttatcaag gaacgttcat tgattatgaa cgttttggtg agagaggcac atataagcat
     3061 atagataaaa attcgacagc gaatcatggc tttaacctaa agatcggtga ccctaagcag
     3121 ataaaatttt ttgagagtag tattgattta cttagctatg ctgcactgaa tcgtgatcag
     3181 ttaaacgata cgtggttagt ctctatggaa gggttaaaac accatgtgat cagtcattat
     3241 tttggtgagg cagtatctga attgagaaag aaacaagcct ttcctcaatc catcgagatt
     3301 tgtgtggata atgaccgtgc aggacatatt ttctacgaaa aagaacaatt aatgggcgca
     3361 gtcgatccat ttacgaatca gaaagtacgt tgtgaacgag gaatcgctaa tgattggcaa
     3421 gtgccaaaag aatacaaaat catttatgaa gaagtcgcaa aagaagaaaa ggtcacacca
     3481 gaagctatta tggccataca caagacagaa aacaatctgc aattaacgaa ccaactggtt
     3541 tccgctcaca aagtaaatgc gttttttggc cagcagcttt ctgttaacga ttcaatagaa
     3601 gccattaatt tgaaagatat ttgtagagaa gtcgcaaaag aattaaaagt ttgtgaacgc
     3661 gtggatggta cgtacgattt tgatcgtttt tatcaagaaa aaggggatat taatgcgcag
     3721 attcttttct cttataaagc agaacaatac tacaaaggct ataaaaatca tgaacatgaa
     3781 tttgtacctg aagtaaaaaa ggactggaac gatcaattga agcatgagat ccagcaacaa
     3841 gaaatcagaa agcaaaaacg tgccatgttg tttcaacaag gcagacaaca agaaagggag
     3901 tgaaatcgtc atgatgaaaa ccgttatttt agcagaaaag ccgtcacaag cgaaagctta
     3961 tgccgatagc ttctctaaag ccactcgaaa agacgggtat tttgaaatcc aagatcgatt
     4021 gtttcctggt gaaactgtga tcacgtatgg ttttggtcat ttagtcgaat tagattcgcc
     4081 cgatatgtac gatgaaaatt ggaaacaatg gtcattagaa catttgccta ttttccctac
     4141 acattatcac tatcatgttc ctaaagataa gaaaaagcaa ttcaaggtgg tgaaacagca
     4201 acttcaatcg gccgacacca ttattattgc gacagatagc gatcgtgaag gggaactgat
     4261 cgcttggacg atcattcaac aagtaggcgc cgatcaagga aaaaccttta aaagactttg
     4321 gatcaactcc ttagaaaaag aagccattta tcagggattc cagcagttac gagacgcaaa
     4381 agagacctat ccaaaatttg aagaagcaca agcacgtcag atcgccgatt ggttaattgg
     4441 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagctt
     4501 ttccttaggc agagtccaaa ccccaacgct ttacatgatt tatcaattgc aggaaaagat
     4561 tagaaatttc aagaaagaac cttattttga aggaaaatcc gaagtaatcg ctcaaaatgg
     4621 cgcatttgat gcgaagcttg atccgaatga aacccaagcg acgcaagaag catttgaaga
     4681 ttatttaaaa gaaaaggggg ttcaattggg aaaacagcca ggcatgattc gccaagttga
     4741 aaccgagaaa aaaagcgcgg caagtccacg tcttttttct ctatccagcc ttcagtctaa
     4801 aatgaatcag ctcatgaaag ctagtgccaa agacactttg gaagccatgc aaggcttgta
     4861 tgaagggaaa tatctaagct atccgcgaac ggatacccct tacattacgg aaggggaata
     4921 tgcgtatctg ttggatcact tagacgaata caagcatttt ttgagggctg aagaaatttc
     4981 gactcctatt catacaccga atagtaggta cgtgaacaac aagaaagtcc aggaacatta
     5041 cgcaatcatt cctacaaaaa cagttatgac agaagcgacg tatgaaagtc tgacaccttt
     5101 acaacaagcg atttacgagc aagttttaaa aacgacagtg gcgatgtttg cggaaaaata
     5161 tacctatgaa gaaacaacga tcttgacgca agtccagcag cttcagttaa aggcaatcgg
     5221 aaaagtaccg atagatctag gttggaagaa gttatttggc aaagagagcg acggaaaaga
     5281 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtagatct
     5341 acaagtcctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     5401 tgcgatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     5461 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     5521 ggaatatatc aaggtagaga aaaataagct tgtggtgacg aataaagggg ttttgctttg
     5581 ccaagctgtg gaaaaagagc cgctgcttac gagtgccgaa atgaccgcca aatgggaaag
     5641 ctacttgtta aagattggag aacgaaaagg aacacaaacc acttttctag ccaatatcca
     5701 aaagtttgtc tctcacttat tggaagtcgt cccaggacaa atccaatcca ttgattttgg
     5761 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     5821 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     5881 gtctgaagcc tgtgatttta aattatggac aacgattgcc aaaaagaaac tcacagcgac
     5941 acagctgaag gagattattc aaaatagccg aacaagccaa ccagtgaaag ggttaaaagg
     6001 tcagaagggt tcgtttgaag caacgattgt tttaaaagaa gacttcacta caggctttga
     6061 attttctgaa aagaaaaaaa cgaacttcaa gaaaagaaca cgacgcacga caaaataatg
     6121 gagggttaat cttatgcgta atacaacaca caaacaacaa gaaatcgaaa atcaaaaagc
     6181 caaaattcgt tcagaacaag agaaattacg tgccagtaaa agaagacttc agcaattaaa
     6241 gaaccagcaa cggattcaaa aagaaatcga attagatcga tagacattaa ccttgttaca
     6301 aatacactat catgatatat ttaatgtagt gtatttgtaa caaaaggggg gaagtaaatg
     6361 caagagaaac tgaaagaatt gttggctatt cgcgatggaa atttatctat gaaggaagcc
     6421 agaaaattag gaatcgcagc aaccactgta caaagattgg tgaatcaagg aaaattgatc
     6481 aaagttgatc gtggttatta tgtagaggat ggtcatggaa tagatgattt attttggctt
     6541 cagcaacgtt tttccagggg aatcttctct catgaaacga ctttagatat ttaccagctt
     6601 tctacgaata tgccgaaatt cattcactta acttttcccc atggatataa cgttgatcgt
     6661 tcaattacaa aagagcagca gttacaattt catttcgtga agaaagaagt ttatgaacta
     6721 ggaaaagtag agggaacttc ctttcaagga aatcctatta ctatgtatga taaagagcgt
     6781 acactttgtg atatttggaa tccgagatat gaaattgaat atgaaatgaa attagaagca
     6841 ttaaaagagt acatggaaga cccattgaga gatccaagta aattaagaga ttacatgaca
     6901 aaactacatg tagaaaaaga aatgaaatac cacatgcaaa gcttatattg atggaggaaa
     6961 aagatgacac cgacacaatt tgcagcaaaa acaagaaatt tagcaaagag gacaggatta
     7021 aatgcacagt tagtacaacg gcattttatg atggatcgtt taattgaaca aatttctgaa
     7081 tctaagtata aaaatgattt cattttgaaa ggtggctttt tattaggatc aaaatatgga
     7141 atcgatagaa ggacgacaat tgatatcgac acgacattta gaaatagcaa attaactgaa
     7201 gaaaaattga agagtatttt taatgattta actaggaaac ccacgaaaga aggtattgtt
     7261 ttctctattc aaggaatgaa ggaaacaaga gaagccgatt tttatcctgg atttcaaatt
     7321 aaattggtgg ctaatttaga gaaagtacgt gtgccattta aaatggacgt gacaacagga
     7381 gattctattt atcctgaagt agatacacac tatcaccaat taatgtttga agataaaaag
     7441 gtggagattc cagcttatcc aacagcacaa attattgcag aaaagctaag tgctacgttc
     7501 agctttggca cagataattc gagagcaaaa gattactacg acttatttac cattccgaaa
     7561 ctagaaaata tcgatacgaa agagttatac aaaagtgttc gaaatacatt tacaaaacgt
     7621 ggggatacaa aaccgttgtc cttttattat gaaagagaaa tggataagat cataggaaat
     7681 gactatttgg aaaagcagtg ggaaaaatat cgctcagtta acacgtttgc gaattccatt
     7741 tcatttacag atacagtcaa cgaaattgat cacctaatga aaagtgtgat caaaattgaa
     7801 aatcaagaaa gaaaacggca gtttaaaaga tcacaacaaa tgaatcaaga aatggagaga
     7861 taaagatgat caataatgta actttagtag gacgcttaac caaagatcca gatttacgtt
     7921 ataccgcgag tggtactgca gtagcgactt ttaccctagc tgtcaaccga aatttcacga
     7981 atcaaaacgg caatcgagaa gctgatttta ttaactgtgt gatttggcgc aaaccagcag
     8041 aaacaatggt gacattagct aaaaaaggta gcttgattgg tgtagttggg cggattcaaa
     8101 cgcggactta tgacaaccag caaggacaac gtgtgtatgt gacagaagtt gtggccgata
     8161 atttccaact attagaaagc aaagcagcga cagaaagccg tgcgcacgct gatcaatcgt
     8221 ccaccagtcc aagcaccacc acttttgaac aacgggatac agcgacgcct aataacaatg
     8281 gattgaacgc tttacagaat ccatttggga atccatttgg tggacaatca atcgatatta
     8341 gcgatgatga tctaccgttc taagtagcat tttctattgg ttgcatttgc tacggtttat
     8401 gttataattt agtcaacaaa aagaattttc aatctataac caaaaaccac tagctggcgg
     8461 gctagtggtt ttcttttacg tttctattat aagaattaac gggtaggctt actgtctttc
     8521 ttggcaaatt tacaatcaag ccatttgcag atatagtgcg cggctacttc tgccatgatt
     8581 gcaaaaacaa aatcaaaaag aattttcaaa cctataacct cctccccttg ccagattagg
     8641 ccggggtagc agacagcaag actattatag cataattgaa atatccaaat gattggattt
     8701 ttgctataat tttagcgagg tgattgtgat gaacaaatat gttggtttgc ttgataaaat
     8761 ccgagtaata aaaacgcaac cgttattggt tcgctttact ttacaaacga ttcatgaatc
     8821 gattaattgt gtagttgcag atattgaaat tatcgataaa ttgctaatta tggatgatgg
     8881 aaaatacaat attgccgtca caggtcactt caataaacga aatcagctag ttattgctag
     8941 catgtatgtt agaaatcccg atcattttac gcgatcgatg ggcatataaa aaaagaagag
     9001 ttgcaatcct aatgtaggac tgcagctctt tttgttttac atagaaacaa gaaaaatgaa
     9061 aaggcctttt taggaaggcg cgagcgcctt ctcaattgaa aaacctctct cactccaacc
     9121 gccaccgccg ttcgagaaga aaggaagtga acgttgcacg ttcactaaga taaagggtct
     9181 tgcaaagcaa gacaaaatag aaaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
     9241 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
     9301 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgattcattc
     9361 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
     9421 ggcagaatca atcatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
     9481 cccaaactat catgcaccac atactgtcga atgcgattgt ggacaaagag cgaaatgtat
     9541 caaaagctat cgtttgtatc aatgtccaac atgcggaaaa aaatacacct tgtcttatgg
     9601 cgattatatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacata acataaatta
     9661 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
     9721 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
     9781 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
     9841 aacatggaaa cttatgaaaa cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
     9901 aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga taaaatggca
     9961 caaaaaatgg aggatatgac attcctatac catgagacac gtgttccaaa aaaacattat
    10021 aaggaaagat tgtcggtagc tgtggaagaa atgattgaat caggtgtcga aatgaatctc
    10081 attgcgacgt actatcgaac actggaagaa ctgaaaaaac aaaatgcaaa atggtttttc
    10141 caagccttac tatgcttgga agtaggtgtc aaaccaagca ctattaaacc ttctgaatat
    10201 caagctttag aactaacgta tgggaaattt gtcgaaacga aaaaagccaa gactgtttca
    10261 agtgaatggt tagattattt tgaaaatatc aataagtatg gcgcctatta caccatgaaa
    10321 aaggaggaca acgaaaatga ataaggtgat ttatcaacta acaacggaag atgtgtcaca
    10381 ctttttaaca aacaaagaac atttgaatga agtaatcaga cggattcaaa aagactttag
    10441 ttgtatggaa gaaatgaagt actttattca aagagtcgaa gaacaattgt atttggaaag
    10501 tgaaacgagt caaacgaagg tcacagtcag caataattat ttagacgtgt ataaacatcg
    10561 tcctgaaacg gtaaaaaaag gaacggagct tctacgacta gaaacagaaa aatacatcat
    10621 gaatgtttac ctagaaagga gtgaactggt cgaaaacttt gtcagctgca cgatttgcag
    10681 gaaacactat tactcagaat cagaatatta ttcaattttt gagagcatgg aattaatgga
    10741 agaagaaatg gaagagctaa acagcaaaaa tgcgacgata gacgattatt atcttgtatt
    10801 tactagattt ttgacgtgtg gaacggaatc tatcgtgtta atgggaatgg attatgaact
    10861 cgctgtaccc gaagcatttt tgagggaata tgtagaagaa gtagataacc caggcgacga
    10921 ctggtacacg actttaactg attttttaga tacctacact tacgacgata cagaagaaat
    10981 ccaaaatgaa cttgaccgta aacgaatgcc atatcaacga tttgaaagga aaaaaaggca
    11041 ataatcattt ataccgaaaa gtgagaatgt gtgaaataag aacaaaaacc ttgaaattaa
    11101 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    11161 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    11221 ttcctctgtt gttcgtactg tgtattggcg agatacttat gcgtcatggt gcagctgtac
    11281 atttaaactt cgtagtattc ttaggaatac tagtattttg tttgattttt tggcgctttg
    11341 tgttagccct tgtgagtcta ctattcaagt tgattgcagc catatttttc atagggttat
    11401 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    11461 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    11521 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    11581 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    11641 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat
    11701 aactatgaaa tctttaaagt atccagatca atttttttct tacctgattc taaagatttt
    11761 gttttcttaa acatattatg atttcctttc gactattttc atataaatgt taacatagtt
    11821 tatggattca tggaatgtta atctaattta atagaaaagg ataattaagt atatacgttt
    11881 tttcttatgt atgttatact atgtatatac agaaagaggt gtctactatg gaaatgttac
    11941 ctataaaaaa atggggaaat agtaatggcc ttagattgcc aaagtatatt atggaatatc
    12001 ttggaattca tacagaagac aaagtgaaga taatacaaga agaagtaaac ggtcagaagc
    12061 ggctaattat cgaagcggct aatatagaaa atgaattaac aattgaacag ttatttgaaa
    12121 attacactga tgaaagaaat catgtgacga ttcaaaattt aggtgaagca gtaggtaatg
    12181 aaaaatggtg agaaaaccta gacaaggcga tattctatta ttgaatacag cacctcgatc
    12241 tggacacgaa cagacaggta aaagacctta tattgtttta agtcatgata ttattgcgga
    12301 ttatagtaac gttgttacag ttgctccaat ttctagtaca actagaaact atccgctata
    12361 tgtaaatata aactcagaat atgaaatgaa gacaacagga aaagtattat tagatcaact
    12421 aacgacaatt gattatgaag ccagagagtg tatttttttg gaaaaagcgc atgatagttt
    12481 agttgaggag ttattgatga aagttagaac agtttttcaa aaagtaagca aataaaatag
    12541 aaacagcagc atgatcaact attcgtgatt gaccatgctg tcttttttta ttctatatac
    12601 ggtttgtctc gtaacattgt agtcttttgc aatcttagcg atcgcaacac ctttttttag
    12661 atcctcaacg atgtttttat aaacaagacg acgttgggga tcttttgcat tggcactgta
    12721 tagcttagga cgtcctttat aaacaccgtt cgcttttgca atcttaatgc cttgtgcctg
    12781 acgtcgttta gattcggtac gttcttgttc agcgatcatg gccaaaattt gaatgatcaa
    12841 atctttaatg aatttatcca ggagaggatt gccaattgct tcggccatga tcggtagact
    12901 tgtgatgatc agctgcacat ttttcttctt caagtaattg actgaagcga tgatctcgtc
    12961 gtaatttctt ccaagtcgat caatagcttc cacaataaaa atatcttgat ctcggacgaa
    13021 atccagtgct tcttgaaaaa caggtcgatg agtaatcgtt gcgccagatt gtttctcaat
    13081 gaaaattctc tctgcaccga attttttcat ttcttcaatc tgccgttgtt cattttgatc
    13141 gatggatgag acacgtatgt atgcaactct caaaattttt acctactttc
//
LOCUS       DACDHL010000066        13186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDHL010000066 DACDHL010000000
VERSION     DACDHL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.066"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..136)
                     /locus_tag="IV228_002022"
     CDS             complement(<1..136)
                     /locus_tag="IV228_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP8717903.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     F"
     gene            complement(384..1220)
                     /locus_tag="IV228_002023"
     CDS             complement(384..1220)
                     /locus_tag="IV228_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8717904.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1231..2160)
                     /locus_tag="IV228_002024"
     CDS             complement(1231..2160)
                     /locus_tag="IV228_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8717905.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2352..2705
                     /locus_tag="IV228_002025"
     CDS             2352..2705
                     /locus_tag="IV228_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8717906.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2779..3435)
                     /locus_tag="IV228_002026"
     CDS             complement(2779..3435)
                     /locus_tag="IV228_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8717907.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3661..5595
                     /locus_tag="IV228_002027"
     CDS             3661..5595
                     /locus_tag="IV228_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8717908.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5681..6904)
                     /locus_tag="IV228_002028"
     CDS             complement(5681..6904)
                     /locus_tag="IV228_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8717909.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6898..8796)
                     /locus_tag="IV228_002029"
     CDS             complement(6898..8796)
                     /locus_tag="IV228_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8717910.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(8790..9908)
                     /locus_tag="IV228_002030"
     CDS             complement(8790..9908)
                     /locus_tag="IV228_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8717911.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9991..10824)
                     /locus_tag="IV228_002031"
     CDS             complement(9991..10824)
                     /locus_tag="IV228_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8717912.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10817..11734)
                     /locus_tag="IV228_002032"
     CDS             complement(10817..11734)
                     /locus_tag="IV228_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8717913.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11961..13085)
                     /locus_tag="IV228_002033"
     CDS             complement(11961..13085)
                     /locus_tag="IV228_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8717914.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4134 a         2650 c         2169 g         4233 t
ORIGIN      
        1 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
       61 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
      121 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
      181 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
      241 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
      301 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
      361 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
      421 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
      481 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
      541 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
      601 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
      661 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
      721 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
      781 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
      841 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
      901 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
      961 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
     1021 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
     1081 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
     1141 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
     1201 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
     1261 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
     1321 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
     1381 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
     1441 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
     1501 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
     1561 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
     1621 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
     1681 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
     1741 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
     1801 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
     1861 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
     1921 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
     1981 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
     2041 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
     2101 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
     2161 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
     2221 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
     2281 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
     2341 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
     2401 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
     2461 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
     2521 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
     2581 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
     2641 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
     2701 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
     2761 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
     2821 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
     2881 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
     2941 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
     3001 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
     3061 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
     3121 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
     3181 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
     3241 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
     3301 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
     3361 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
     3421 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
     3481 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
     3541 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
     3601 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
     3661 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
     3721 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
     3781 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
     3841 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
     3901 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
     3961 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
     4021 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
     4081 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
     4141 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
     4201 caacaccctg acattttgat tctagatgaa ccaacgaacc atttagatat cgagacactt
     4261 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
     4321 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
     4381 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
     4441 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
     4501 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
     4561 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
     4621 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
     4681 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
     4741 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
     4801 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
     4861 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
     4921 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
     4981 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
     5041 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
     5101 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
     5161 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
     5221 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
     5281 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
     5341 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
     5401 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
     5461 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
     5521 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
     5581 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tagtttttct
     5641 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
     5701 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
     5761 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
     5821 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
     5881 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
     5941 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
     6001 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
     6061 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
     6121 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
     6181 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
     6241 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
     6301 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
     6361 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
     6421 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
     6481 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
     6541 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
     6601 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
     6661 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
     6721 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
     6781 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
     6841 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
     6901 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
     6961 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
     7021 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
     7081 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
     7141 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
     7201 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
     7261 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
     7321 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
     7381 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
     7441 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
     7501 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
     7561 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
     7621 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
     7681 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
     7741 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
     7801 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
     7861 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
     7921 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
     7981 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
     8041 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
     8101 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
     8161 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
     8221 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
     8281 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
     8341 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
     8401 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
     8461 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
     8521 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
     8581 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
     8641 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
     8701 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
     8761 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
     8821 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
     8881 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
     8941 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
     9001 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
     9061 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
     9121 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
     9181 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
     9241 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
     9301 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
     9361 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
     9421 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
     9481 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
     9541 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
     9601 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
     9661 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
     9721 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
     9781 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
     9841 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
     9901 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
     9961 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    10021 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    10081 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    10141 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    10201 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    10261 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    10321 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    10381 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    10441 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    10501 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    10561 actggctgta gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    10621 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    10681 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    10741 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    10801 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    10861 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    10921 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    10981 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    11041 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    11101 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    11161 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    11221 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    11281 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    11341 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    11401 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    11461 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    11521 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    11581 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    11641 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    11701 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    11761 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    11821 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    11881 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    11941 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    12001 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    12061 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat tttttgcatc tttagggaca
    12121 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    12181 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    12241 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    12301 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    12361 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    12421 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    12481 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    12541 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    12601 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    12661 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    12721 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    12781 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    12841 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    12901 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    12961 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    13021 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    13081 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    13141 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttac
//
LOCUS       DACDHL010000067        13148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDHL010000067 DACDHL010000000
VERSION     DACDHL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.067"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(78..150)
                     /locus_tag="IV228_002034"
     tRNA            complement(78..150)
                     /locus_tag="IV228_002034"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="IV228_002035"
     CDS             531..1100
                     /locus_tag="IV228_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8717915.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="IV228_002036"
     CDS             1087..1947
                     /locus_tag="IV228_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8717916.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="IV228_002037"
     CDS             2137..2841
                     /locus_tag="IV228_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8717917.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="IV228_002038"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="IV228_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8717918.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="IV228_002039"
     CDS             4678..5994
                     /locus_tag="IV228_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717919.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="IV228_002040"
     CDS             5995..6864
                     /locus_tag="IV228_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717920.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="IV228_002041"
     CDS             6919..7728
                     /locus_tag="IV228_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8717921.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="IV228_002042"
     CDS             complement(7831..8460)
                     /locus_tag="IV228_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP8717922.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="IV228_002043"
     CDS             8630..9922
                     /locus_tag="IV228_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8717923.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="IV228_002044"
     CDS             complement(9998..10660)
                     /locus_tag="IV228_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8717924.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /locus_tag="IV228_002045"
     CDS             10885..11169
                     /locus_tag="IV228_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8717925.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="IV228_002046"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="IV228_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8717926.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4371 a         2265 c         2833 g         3679 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aattataatg aaaatagtta tttttaatag ttataatatt ttatctatta aaagtaaata
    13141 tccattca
//
LOCUS       DACDHL010000068        13084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDHL010000068 DACDHL010000000
VERSION     DACDHL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.068"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(79..2199)
                     /locus_tag="IV228_002047"
     CDS             complement(79..2199)
                     /locus_tag="IV228_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8717927.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="IV228_002048"
     CDS             2429..3607
                     /locus_tag="IV228_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8717928.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="IV228_002049"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="IV228_002049"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8717929.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="IV228_002050"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="IV228_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8717930.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="IV228_002051"
     CDS             complement(4960..6213)
                     /locus_tag="IV228_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8717931.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="IV228_002052"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="IV228_002052"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8717932.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="IV228_002053"
     CDS             7790..9136
                     /locus_tag="IV228_002053"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8717933.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="IV228_002054"
     CDS             complement(9444..9830)
                     /locus_tag="IV228_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8717934.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10709..10921
                     /locus_tag="IV228_002055"
     CDS             10709..10921
                     /locus_tag="IV228_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717935.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="IV228_002056"
     CDS             complement(11199..12998)
                     /locus_tag="IV228_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8717936.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4290 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 ttta
//
LOCUS       DACDHL010000069        12937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDHL010000069 DACDHL010000000
VERSION     DACDHL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.069"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            415..888
                     /locus_tag="IV228_002057"
     CDS             415..888
                     /locus_tag="IV228_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8717937.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            897..1124
                     /locus_tag="IV228_002058"
     CDS             897..1124
                     /locus_tag="IV228_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8717938.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1760..2131
                     /locus_tag="IV228_002059"
     CDS             1760..2131
                     /locus_tag="IV228_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717939.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2387..2629)
                     /locus_tag="IV228_002060"
     CDS             complement(2387..2629)
                     /locus_tag="IV228_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8717940.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2661..3563)
                     /locus_tag="IV228_002061"
     CDS             complement(2661..3563)
                     /locus_tag="IV228_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8717941.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3576..3764)
                     /locus_tag="IV228_002062"
     CDS             complement(3576..3764)
                     /locus_tag="IV228_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8717942.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3778..4341)
                     /gene="amaP"
                     /locus_tag="IV228_002063"
     CDS             complement(3778..4341)
                     /gene="amaP"
                     /locus_tag="IV228_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8717943.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4379..5272)
                     /locus_tag="IV228_002064"
     CDS             complement(4379..5272)
                     /locus_tag="IV228_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8717944.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5350..6288)
                     /locus_tag="IV228_002065"
     CDS             complement(5350..6288)
                     /locus_tag="IV228_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8717945.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6322..6672)
                     /locus_tag="IV228_002066"
     CDS             complement(6322..6672)
                     /locus_tag="IV228_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8717946.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6705..7607)
                     /locus_tag="IV228_002067"
     CDS             complement(6705..7607)
                     /locus_tag="IV228_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8717947.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7600..8457)
                     /locus_tag="IV228_002068"
     CDS             complement(7600..8457)
                     /locus_tag="IV228_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8717948.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8791..9600
                     /locus_tag="IV228_002069"
     CDS             8791..9600
                     /locus_tag="IV228_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8717949.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9640..10137
                     /locus_tag="IV228_002070"
     CDS             9640..10137
                     /locus_tag="IV228_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8717950.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10522..10595)
                     /locus_tag="IV228_002071"
     tRNA            complement(10522..10595)
                     /locus_tag="IV228_002071"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10559..10561),aa:Ile,seq:cat)
     gene            10782..11105
                     /locus_tag="IV228_002072"
     CDS             10782..11105
                     /locus_tag="IV228_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717951.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11269..11523
                     /locus_tag="IV228_002073"
     CDS             11269..11523
                     /locus_tag="IV228_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717952.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11593..11838
                     /locus_tag="IV228_002074"
     CDS             11593..11838
                     /locus_tag="IV228_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8717953.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11914..12279
                     /locus_tag="IV228_002075"
     CDS             11914..12279
                     /locus_tag="IV228_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717954.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12340..12903)
                     /locus_tag="IV228_002076"
     CDS             complement(12340..12903)
                     /locus_tag="IV228_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717955.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         3770 a         2403 c         2180 g         4584 t
ORIGIN      
        1 caaataagtg gtgattacac caaaccattg aataagaaaa attctgatac gtaaataaac
       61 atgaacttga aaaaattaat gaatcggtta gctactacgt agttgtcttt tttctcatac
      121 agttctgtta acttacactt ctcttcgcta gataataaaa aatcctccaa ctgtattgtc
      181 caatataatc ccaggcatct aaactagcat tatagaagag acacacatag aatctaaaat
      241 aactggtttt aaatgtttct tacaatcaaa aaaagctgtt aaatgcactc cacaccttca
      301 tactctatca ttttttataa acgatatatt tttattgttt ttcgataaaa aatagttgtt
      361 ttacaataat tattctgcta tattttagct atcaaagata aaagagggat aaaaatgaaa
      421 tttaaaacat tgctattaaa atttgtcgtt ttaggtataa gtgccgtatt tattttgttc
      481 ggcgtacttt tttatactca gatcgctaca agcgaaaaag gattcaattt tgattggcag
      541 accacattat ttgtttctgt catttacttt gcccttctcc tagggatcgt tattgcttat
      601 ttcttattga aactattgaa aaaaatcgaa gataaccaag ctttttccac tcaaagctta
      661 aacatactaa aaaaaatcga acgaaccatt ttgtttattg gtttcgcctt ttttggctta
      721 cttcctaaat ttatgaaagt agcggattta gacgatgctc caggtttgat gttggtagga
      781 atcgctgtcg tattcattcc ttttgcagta tacacattaa caagcgtgct ggaaaaactg
      841 cttgaacatg cgattttact taagacagat catgatttga cagtgtgagg tgcgacatgg
      901 caattatcgt taatttagat gtagtattag ctcaaaaaaa aatgagtgtg actgaattga
      961 gtgagaaagt gggcattacg atggcaaaca tctctattct aaaaaatgga aaagcaaaag
     1021 ccatccgttt ttccactctc gaaaaaatct gccaagtttt ggagtgtcaa ccaggtgatt
     1081 tgttgaccta taaaaatgac tcgaatacta taccagaaga ataaaataaa gtagaacaaa
     1141 cagtaaataa aaaataatct gaatgataga tgtgtgagac ttttagaagt tatttacgtc
     1201 tattattcag attttttatt tcgattgaat accgttttat ctataaataa gatatgtact
     1261 gactcataca aagggggcct tttcttctcc atgtactatc acttttcctg atacaactgg
     1321 tttcctgtta cgttgtagtt ggatctgctt ttagtataat actttattca ttccaccaga
     1381 ctgatagcag tttgagcagt ctaaataatc atgtagtatt acaggccatt ctgtttcata
     1441 ccttgaagtg cttaaaactt cagctaatgg cccattgtgt taactcttac tccattcttt
     1501 tttagtaagc tcccatatat actgtaaagc cttctatata ttatgtcatg ttgcttttta
     1561 acatttacta tgtagttttg ataaaatgat ttgaataaca gtgatggaca attctttttt
     1621 atttttaaca ttattttgat aataatgatt atttatttaa aaatacatta gataacattt
     1681 gttacgacac gaaaaagctt cacaaacaaa tatcgttatt ttaaaatgag ggcgaactca
     1741 cacaattagg aggaatagta tgccatttat tgtttacttt tttctttcat tactcgtgat
     1801 ctatattcca ttaccaacat ttttcctgct cgtcaatcgt ctagcagagc aacatgatac
     1861 gattgctgct cttacgaaac tttgtttact tctatttgtt tgtctagatt actttacgag
     1921 ctattattgg ctaaaacaga caagagtaaa aaagagatat tatttcttcc ttgtcttttt
     1981 caatctcttt gagtttctga tccattgctt tttacttcat cagtatcata cgatccattt
     2041 gtggatcatc tgggttttca aagtggtcgt attattttgt atgttcacct ttcctatctc
     2101 aagaaaattt agggaggcat tgttttatta aagaagcttc tctgtatcaa taaagacaca
     2161 tttaaggaaa aacttttaaa aatctaattg ggaatttgcc cgcataaata agaggctgtg
     2221 acaaaagtct tgtcacagcc tcttattccg tataaacagt gttcaaaagc ttgcatcttc
     2281 gataatatgc ccaaccaaat gaaaaatatg agaagctatt tttgtttggc tgttcttatt
     2341 acttgatgaa gaacagcttt ttcacgacct ctttttaaaa gtctttttac agttttcgag
     2401 caaagaatga tacgattagt actaaaatga tggcaccaat caatgatggc acgattgcca
     2461 ttcctgcaat acttggtccc caagaaccta ataatgcttg accaagtgct gatccaacta
     2521 aacctgctac gacatttgca ataatcccca tcgaagaacc tttattcgtg attgctcctg
     2581 caattgctcc gataattcct ccgacgataa gtgaccaaat aaatcccatg tttcgttcct
     2641 cctaatgttt tacttcctta ttactgaacc cgtggttctg tattttcttt tactttttgt
     2701 gcgccgcttc cagctgctga ttttacttta tctgtttgat tcgaagcaaa ttcaccggtt
     2761 tttttcgctg cgtcagttac tttgtcttgc accgtttctt gatctttttc aaattcttct
     2821 tttgttttga tgtccactac gttagcgttg acttcgatca cttctaaatg tgtcatattt
     2881 tttacttctt tgccgattac ttcttgcatt tcttcaaaga ttttttcgat atctttgcca
     2941 tattccacaa tgacatccat atcaactgct acttgttttt taccgacttc tgtttcgatc
     3001 ccagccgtta cattatctgt attcacaagt tttcctgcca cattagagaa gaaaccaccg
     3061 tttgcagata ataaaccgtc tacttgttca attgcgattc cgatgatttt ttgcacaact
     3121 ttatcttcat aattcagctc accgcgcatt gaatcttcct gttcagaatc atgctttcct
     3181 tggttatttg tatgatcaga accaagtttt ccttgtccgt ttgtgtggtc agaactaagt
     3241 ttttcttgtg ctttcgcatg gtcagaagga tgtttttgct cttccacagg tttagaagga
     3301 tcgattgcat catgttgttc tggaacttta gattctggag ttcctggatg atgaggtgca
     3361 tgcttttctg catcatgttt cttctcttct actccttgtt gaacgttagg gactactgga
     3421 tctggatgtg cagatactgg tgctgttttg ttcttttcat cgttcacacc atttgattgg
     3481 tttggtgtcg ttgggtctgg atgtgcagac actggttttt ggttgttcat tttattgtta
     3541 ttatttttgt tattgttatt catggatgtt tcctcctaag atttaaaata gtgatctaag
     3601 attttcgttt gtttcagata aaaaccgata gcgacgccga tcgctgccat gatcaagatc
     3661 aataaggtct tgaaaaagcc cacggtgatc cataaaacgg ctaatatcaa tccaagtacc
     3721 ccaaagatga tagggagctt gtatgtcgaa aatagttctt tcatttttgg tcccccccta
     3781 ttctactcgt ttgcggtcat tgcttcgttt agctggttct tcaaagcctg tgtatttcac
     3841 cgatactttc actggctctt ttgttcccaa tatgtgttgt acttcatttt cgatttcttt
     3901 catcaatgtt cctgttttac caatgagtgc tgaagttcgt ttcaactccc cttttacatt
     3961 gacggcgatc ttgtttttcg ttgctttgac atttactttt ggtgaatgga tgaattcatc
     4021 ttcatgcaag tgagaacgaa ccattccttc aattgctttt ttatccaacg tcaactcccc
     4081 atcaccatgt ttcagtagaa agtttccgat tgtttttgga taaaacagga aaacaagtaa
     4141 cgtgataatt gtcaaaatag caaatgcaac ggcaagccaa aatacatatt gttgcacgta
     4201 tgaattagtg atggaaaaga atcgaaactt ctctagctga aaaggcaatg gataggttgc
     4261 acttaatagt atcgcaaaca aaagcacaga tagcaaaagt aatgttccga tcactccgat
     4321 cgtttttaca cctttattca ttttcgcacc tcctcatagg ggtgaaacga tcaacttctt
     4381 ataagttgat cgcttctcca ccagttactc cgtaaatttg ggctgtaaca taacttgctt
     4441 tattcgatgc taagaaaaca taaactggtg ctaattctcc tggctgacct gcacgttcta
     4501 gtaaactttg ctgaccaaat tcagggatcg aatcagaagg ttgtccttcg tctagttgta
     4561 atggtgtcca gattggacca ggtgctacac catttacacg gattccttta ggtgcaagct
     4621 gttttgctaa accgatcgta aagttagcga ttgaagcttt tgttgctgcg taatctaata
     4681 aatgttcgct tggattgaat gcttgaacag aagttgttgt tacgattgct cctcctgctg
     4741 ggatatgcgg aacagcttct ttcactaaag caaacatagc gatgatgttt atcataaatg
     4801 tgtctttgac ttgctcaagt ggtaactcag caatagactg acaagaaatt tgctgtcctg
     4861 cgtttagtac catcgtatct agcccgccaa gttcactcac ggctttatct atgatttctt
     4921 taggtgcttg ttcgtcacga agatcaaaag gtagtaagac aatcttacgt cctgcaactt
     4981 tcacgtattt tgctacttcc tctgcatctt tttcttctcc tgggaagaat tgaagagcga
     5041 tatctgcccc ttctctagca aatgcaatcg ctgctgcacg accgatccct gaatctccac
     5101 cagtgattag tacacggcgg ttttctagtt gattattgcc ttcataggat tcttctccac
     5161 aatcaggaac aggaatcata ttctgctgta aagctggtgt atcctgatct tgtttaggaa
     5221 atttctccgt atggtacaag tttcgcgggt cgattaattt tggttctgtc attgctgtat
     5281 tcctcctagt ttttaaattg tattgccaaa acaaatttct ttgtttgata cctgttttaa
     5341 atagattctt taatttgtat aatctttccg gtataaatgg taacctaaaa ctactgctgc
     5401 tatcaaagag ccaaagacta cgagtataaa gcctagagca gaacttttac ccggtaatcc
     5461 tcctgtattc attccccaaa tccctgcgaa caacgccgga acggaaatga ttaatgcagc
     5521 tgaatctaga tatttcatga tatgatttag attattgtcc atcatcccgt tgaatagatc
     5581 accaatactc tctaataaat ctcgatagat ctcgatcatt ttttctgttt gtcgctgtct
     5641 caaatggaca tcatagagta gctgttcatc gtctaaacgt ttgatgaaag tttcagaatt
     5701 ccataattga tccattgttt cccgttgatc tcttaaggta tgatcgatat atacgatgtc
     5761 tctttcaaga tctgcctgtt tatacagttc ttcattttcc gttgttttac gagctgcttt
     5821 atccaaagcg tcaattcgct ttttcatctc tcttagttct ttcacgtaat gggtgtaaat
     5881 atctaataat gtatacgtga tgatcttttc aaacgagttt aaatgatttc catgctttgt
     5941 cagcagccga tccacaaatt cggtttttct atcgatacaa gtaatcaaga gattatcgga
     6001 aagtacaaaa gatattggcg ttaatttttt ttcgatacga ttatctgact gatctagatt
     6061 aatcactacc aaagaaaatg gatgctctag tttcgtctca taaaggcgct ccatccgaga
     6121 aacttcttcc ggatattcgg ttcctacgaa aatatcatca ggaagctcga attcttcgat
     6181 tgcttgctca atctcttcgg gctttgcact atcaagtacg agccaattat attttttttc
     6241 atctgtttct attacattct tttcttcatc cacaaagtag tatctcaatg tatacctcct
     6301 ttttggactt tctcgaaact attagctgaa gaagcagttc tttaatttga tattataatc
     6361 gagtactgcg atttcatttt ctaatgaaaa gctgtcaagc tgtttgttga agtccatgtt
     6421 caatcgttta ccgaaataat tttgctctaa tttagatgta tagatgtcca ataatggatg
     6481 ctcgatttta gtcgcatatt ctccaaattc cgaaacaaaa ggaatgattt gaaaaaagct
     6541 gccttttgca aaagcacctt tgtcccttag aaatggatcg gaatcattga taagcgcgag
     6601 tgcgattttt gtattttccc caaccttttc aattaacgtc tctactgcat ttttagacat
     6661 tgtaatttcc attcctactc cccctcttca gttgttgtct tctattattt aacaactaca
     6721 tctgtttttg gttgaacttt tgcatatttt ggatatttag ggaatttagc tacacaacgg
     6781 aaagccgtca tccctgcata tttttcgtaa tatttcttcg ttggcccttc aaaatccttg
     6841 ctccacggtt tcacatgtcc tgaaaaatgg ataatgtaag gattgttgcg agtttcttcg
     6901 tattcacgtt cacctgctgc tgttggatgt ttttttgctt ttgccatgat atagccttgt
     6961 gcattccaac gcggatgaag tggtaaccaa cggtcatgta agatagcatt taatgcatct
     7021 tggtcatgga atcgtaattt ttctggatta tgttcgatga aatctaatac tttttgcgta
     7081 atattttcat ctaaccattt tttcacattg atcagcatca aacctgagtt gaaataacgc
     7141 attgatttag ctgaaatttt cattttttcc agtcgttgat ggaacccagc gtcttcaaca
     7201 gctgctacga ttgaatcacc gaagtcaaga cgccataatt tagagatatc ttgtaaagcg
     7261 atcatgtcgc aatccatata taagatacgt tcaacttctg tgccgcggaa caattccgga
     7321 atcgcaatac gataataagc tgtttctgga atacgatcgc tgattaccac atttgtaaag
     7381 aattctttat tgattttcaa gaattccaca tctgagttca ttcgtgcgtt tttcactgag
     7441 aagcgtaaaa gctgtttact ttcaaaatcg atatcatcgt caatcacata gaaccgaaca
     7501 aatttagacg ggttgcaatt atctaatact gagacaaata atgcagctaa atgtggtacg
     7561 aattttgtgt tacaacttgc gactactgct atttcttttt tattcattga tctcgcctac
     7621 tttcgttaga gtactgtgtg ctaatttttt taagtataaa ttggtatacg gatgatcttt
     7681 caacgtattc caaggtttat catgtcctgt aaagtgaacg atcgaaggtt tttcatttcc
     7741 ttctttatac tgacgttcat atttttcatt tggtgctgga tgacgttcaa aaatcagcga
     7801 agtctgcatg ttccattttg gatgaagttg ttcccaatct tcataaagta cagcattcaa
     7861 agcatcttgg tcgtgataga tgattcgatc gccattttca ctgaggtaat ggatagtttt
     7921 ttctgtaatt tcttttttat tccattgatc aatgtcaatc accatcactc ctgaattgaa
     7981 gtacaagtca tcggaatcga ttcctaatct ttccaaagct ttcacttggc ctggatcgat
     8041 aatagcgccg attgtttttc catttaacgg ctcatcatat agttttacga tatcgtctag
     8101 tactaacaca tctgaatcta agtaaaggac tttcttataa tcctctttag ctaataagtt
     8161 tggtaaagaa attcttaagt aagcggtagt tgtgatatgg tcactgacta ggaagtcttc
     8221 gtagatatct ttttccactg tcaggaattg gatacttgca ttacttgaat atttatttac
     8281 cgtttcttcc aatccttctt tactatattc tgatagacca tcgtcaatca cataaaattt
     8341 tatgcgtctt gttttattgc aattttctaa tgcagttgcg atcatcacgc tcaagtaagg
     8401 agcataattt tcgtctgaag cagttaccac aggaactacg ccgtatctct tctccatcaa
     8461 aatcactcct ttttctaatc tttttctaac ctgttctaca tctagaatat tcctcatttg
     8521 gttttaaagc aaaatatatg ctttgttttc attgaatatt ttaaaatttt tttatcacaa
     8581 aacgcatcgg taagcgctaa ctattcgttt ttcatggctt tttattcaaa ggtattgacc
     8641 aaaaactact atatatagcc acctttttct taagaatgtt ttttgaaaaa attcataatg
     8701 acaaagcaag ttagtttcat ttttttaaat tctcatttaa tcttaaggta acaggaaaat
     8761 agatatgtaa aaagaaagta ggaagataaa atgaaactag cagcgattga tttagacggt
     8821 accttgcttg atcgaaatgg aaagatacca gaagaaaata tccaagcact tcggaaattt
     8881 gatgaaaatg gcggaatcgt aaccgtcgct accggaagga acagcatttc tgctaaagat
     8941 attttttccg aattaggaat cggtggctat ttgatttctt ccaatggttc actgattgct
     9001 gaaatgaagg atggaaaaat ggcgaaagtt ctcagacgtt caaaaataga tagcgataca
     9061 ctgagaaaag ccttcttcta tgctcaagaa gcgaaaatat ccatcattgc tagccgagaa
     9121 acccaagatg atcagctgac attcgatgaa tcaaatctag tcaaagatga cccttattat
     9181 gaacatttca atttacagaa ccattctttt gatcaaattt taaatatgtt ggaagatcct
     9241 tctcttagct atcttaaatt agcattaaca gataagagtg aagaaaaact acagaagcta
     9301 caaagagatc tgcaagcaga tggtatccaa tcagtattct ctgatccgca tttcttagaa
     9361 atcacgccta caggtatcac taaagctcat tcacttgttt ttctggcgga ttaccttgga
     9421 atcgaatcga gtgacatcat ggcttttggc gatcaagaaa acgacattgc tatgttgaat
     9481 tacagcgggt tgagtattgc gatggggaat gcacagcgac aagtaaaaga aattgcaaac
     9541 caagtaacac tgacgaatga agaagctggt gttgcttata ttttaaaaaa cttctattaa
     9601 aaagaataaa atgacgatat gaatgtagga ggaaagaaaa tgttagttat tattgatatg
     9661 caaaatcata ttcttgatcc aaacagcgaa ttttatattc cagagtcaga tcaattggca
     9721 gaacgtattg ctcaaagatt agctaaagca cgccaaaata atgaatatat tctcttcaca
     9781 cgggatatac cagttgaact gaaggacaaa gaagaagaac gagaagattt gcaattgatc
     9841 cctcttcttt ctccacaaga aaatgaacga gtgattaaaa agtattactt tacgattcca
     9901 cctgaaactt tagctgaaat ccaggaaact ttatttgaaa gcaaagaaga acaaaaagag
     9961 atcgaagtag tcgggattga aacaaacttg tgtgtactct ctaatctgat aggcctgcag
    10021 agtgcttttc ctgaagcaga cttttttgtt gatccaatgt tagtctccag tagaaaacac
    10081 ggagaatccg ctttagagct gttgaaagat ttcaacgtct ctatcgtcga atcataaaaa
    10141 tatctatctt catttacgat atttaacaca caataaaaaa agcacgcaga actgttttag
    10201 tcctacgtgc tttttctatc aaagtagatt ggctttttta tgacacagag tgaaacggca
    10261 ggctcacgac ctctttctta aatggtgttc aatcagctga ccctcggtat tagcttaaca
    10321 aacggcaaaa tatgaggaac tatttttccg tttgttaagc taatactcag gtcaaaacgc
    10381 tgatttcaca acctcttcct taaacggtat tcaaaagctt gcatctgcag taataagaac
    10441 agccaagtaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
    10501 cagctttttc acaacctctt atgtaccttg taggactcga acctacgacc ggacggttat
    10561 gagccgtctg ctctaaccaa ctgagctaaa ggtacatcat aaataaaatt tatttttgtt
    10621 acttttcaac tatagcacga atgacaaact ctcacaagtt tcaaaatata agtaaaataa
    10681 tattgttatt cttatttctt ttacttgtgt cataactatt ttgttacttt aatttttatt
    10741 tgcttatttt agaaaaaatg ttattctttt atttattctc aatgttagga gtgattattt
    10801 tgccatttat tgcgatttta ttcgatttag ttgcggcagc agcttatttt ctccaattaa
    10861 attatcaaac ctcaggaatt ctgtttattg gaatgatttt tcaaggtgtg attacccttt
    10921 tgcttcttgt catgatgctc agctataaag gaaaaagata tgcccgtatt caaacaaaag
    10981 tatttgttaa atatgtttcc attcgctatg ggatcatcat tctttccttt ttagtgaatg
    11041 cgatcgtcct ctttttatat gtattgaatt acttagatat caatccattg attttttctc
    11101 gatagataag acacattcag cggaacagtt cctgcttatc agcagaaaac tgttctgctt
    11161 tttcattttt cattgatcta ttcttctttt attttcctag catttcattt gtgatgtatg
    11221 caataacatg ttatctttta ttatatgaat cgttgaggag tccgttacat gattacgttg
    11281 gtgagagttt tgttttgggt gcctgcggtc gcactggtcg caagtattgt ttatcttatg
    11341 aattggaaca aagaacggtt ttatttagct attttgactc ttccggctat ctactttatg
    11401 tggaaagttt ttaattacaa ctactttgaa cccgatagtg tttttataga agaattatca
    11461 ggactggttt tgtctttatt gatcgtcatt ctttatttga tacgtttaaa taaaaaacat
    11521 tgagtactat tgattgcatg tgtagctttc tatagtcatt ataactattc tactataatg
    11581 aaaggtgatt aaatgagtat cgaagaagct attttactgg atactttacg ttgtcgtctg
    11641 aatttcttaa gaactggccg atctttatct caagtagctg tttctagcgc gttaagtatt
    11701 cctttattca aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat cattgaatta
    11761 atctatatcg ctgactttta cgatatttcc gttgattacc taattggtcg ctcagaaaca
    11821 gcaagcaaac tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt taacgtctaa
    11881 ggttaacaat ctatatataa aggagacatt tctatgcgat tatggcatga aaaattgatt
    11941 tcaaagcttc ctcgtcaaca gcttttagga caacatcggg aatgctgtgc attaagagga
    12001 aatggctggg gaaagaaaca tgctactgtg gattatgttt ttcactattc tccttataga
    12061 ttattccaat atcatcagct tgtcatggat gaaatggaaa aacgtggtta cagcccagca
    12121 ccagagtgga aaaatcctat gtatcgtggc aaatcatgcg aagcattttt tgatttacct
    12181 actgtagagc gcaacgtgcc gatttatccg gaacacgatg aagtttatct agcagaatgt
    12241 ttagcaaatt tggagcaaaa aggaatttat cttacctaat ataactagaa aagccttcta
    12301 tctcctctta cttcattaaa aagctttaac catcttagat caagctaaaa tgtcgtaagt
    12361 tttagggtta tcttcatcct cacgtgcaaa actaaaagcg tactctagtt ttggttcatc
    12421 gccaacttcc ctagacataa atgaacatct cctgacgtgg tttaaaagta ccgcatcttc
    12481 gacgtctgta tacgatgcac agcacagatt gcttcttttg atctttcttt tgatttttct
    12541 gttgcttctt ctacaccttc ttcgattact acatcgatgc ttacaattgc ttctgcctaa
    12601 tattcccatg catcgtcgat ttaagcctct gtagtcactg tgatcgtttc cacattttaa
    12661 ctcctctgtc gtggcttcag ttgattttgt gatcagttca gttgctaagc cagagtcaga
    12721 acaacctgcc attgctagcg ttaatattga tagtaaaaat agttttttta tcataaggtt
    12781 ccccccctga aaatagacta aaataataac gctttctttt tttgttttca gacgaaaatt
    12841 aataatttct aaatattctt taaaaaagct tataactatt atgccttttt taatatataa
    12901 cacagttatt tttaaaaata agaatgctaa atcactc
//
LOCUS       DACDHL010000070        12871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDHL010000070 DACDHL010000000
VERSION     DACDHL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.070"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IV228_002077"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IV228_002077"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8717956.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="IV228_002078"
     CDS             complement(2424..2675)
                     /locus_tag="IV228_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8717957.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="IV228_002079"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="IV228_002079"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8717958.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="IV228_002080"
     CDS             3624..3842
                     /locus_tag="IV228_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717959.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="IV228_002081"
     CDS             complement(4130..4642)
                     /locus_tag="IV228_002081"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8717960.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IV228_002082"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IV228_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8717961.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7529)
                     /locus_tag="IV228_002083"
     CDS             complement(7095..7529)
                     /locus_tag="IV228_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8717962.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="IV228_002084"
     CDS             complement(7539..8327)
                     /locus_tag="IV228_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8717963.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /locus_tag="IV228_002085"
     CDS             complement(8324..9265)
                     /locus_tag="IV228_002085"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8717964.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="IV228_002086"
     CDS             complement(9442..10029)
                     /locus_tag="IV228_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8717965.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IV228_002087"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IV228_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8717966.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="IV228_002088"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="IV228_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8717967.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
BASE COUNT         3561 a         2881 c         2280 g         4149 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga t
//
LOCUS       DACDHL010000071        12375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDHL010000071 DACDHL010000000
VERSION     DACDHL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.071"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            426..983
                     /locus_tag="IV228_002089"
                     /pseudo
     CDS             426..983
                     /locus_tag="IV228_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1041..3080
                     /locus_tag="IV228_002090"
     CDS             1041..3080
                     /locus_tag="IV228_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8717968.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3189..4340)
                     /locus_tag="IV228_002091"
     CDS             complement(3189..4340)
                     /locus_tag="IV228_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8717969.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4494..5024)
                     /locus_tag="IV228_002092"
     CDS             complement(4494..5024)
                     /locus_tag="IV228_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717970.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5123..5473)
                     /locus_tag="IV228_002093"
     CDS             complement(5123..5473)
                     /locus_tag="IV228_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8717971.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5630..7159)
                     /gene="ahpF"
                     /locus_tag="IV228_002094"
     CDS             complement(5630..7159)
                     /gene="ahpF"
                     /locus_tag="IV228_002094"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8717972.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7169..7732)
                     /gene="ahpC"
                     /locus_tag="IV228_002095"
     CDS             complement(7169..7732)
                     /gene="ahpC"
                     /locus_tag="IV228_002095"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8717973.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7876..8538)
                     /locus_tag="IV228_002096"
     CDS             complement(7876..8538)
                     /locus_tag="IV228_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8717974.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8722..10368
                     /locus_tag="IV228_002097"
     CDS             8722..10368
                     /locus_tag="IV228_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8717975.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10359..11441
                     /gene="uxuA"
                     /locus_tag="IV228_002098"
     CDS             10359..11441
                     /gene="uxuA"
                     /locus_tag="IV228_002098"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8717976.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11549..>12375)
                     /locus_tag="IV228_002099"
     CDS             complement(11549..>12375)
                     /locus_tag="IV228_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8717977.1"
                     /translation="YEYIDFFIDLSVDILRPNLPICREQRILVNILARLSITKIESEE
                     TSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLN
                     FNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDL
                     VHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISD
                     CPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         4132 a         2284 c         2322 g         3637 t
ORIGIN      
        1 gttatttttt tctaaaaaat attaaagatt gttaaatttc actatttcat ttcaaattcc
       61 attgcctaat cattaggaaa agtctatcat gaactcgtaa atagaatata gtaataacct
      121 tgttaggtga ggctcctata caaacatagg ctgttgccca gaaacgtcga aagtcgccaa
      181 tgggtaaaac agaaactgtt ggattaaggc tttttccaat agctaagaat gttttcttta
      241 cgttgtatag tgctaaaact cggacgatga gatgatgcta ggtcagaatt cttgttcgaa
      301 aaggtttctg accttttctt tatatttttg tgactgactt atttgatgag acaaactcgc
      361 tcctcacctg acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa agatcgattg
      421 gtggaggtcg ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa
      481 aactgatctc tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga
      541 taaatgcagc ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt
      601 tcaaatttga agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta
      661 atgaactgtt tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg
      721 tatatataaa caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat
      781 ttcgggaaga caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag
      841 ctatctctca gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga
      901 aattacttgg tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg
      961 atttcctcaa agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca
     1021 agcaagagaa ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg
     1081 cttatattgc cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc
     1141 agcactatca agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc
     1201 aagctttgaa caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa
     1261 ataaaagtgc attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg
     1321 ctgatgtcaa aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt
     1381 acagtttttc atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt
     1441 ataaaggaac attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg
     1501 tttttccaga aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag
     1561 ggaatattat agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag
     1621 gagtcgttcc aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg
     1681 cttcctcctt cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac
     1741 aacccgatta ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg
     1801 gagctaccat tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc
     1861 tgattggtta cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca
     1921 gtaatggaaa aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga
     1981 caacaggtgg gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg
     2041 aacatgaagt tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa
     2101 tagcttttga cagtctagga ggaaaagctg catcaactgt tgcgacaacg ccaaaaaccg
     2161 gtgatcttct tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga
     2221 tctcacaaga agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat
     2281 ttgcgacagg ttatgctcct ggatctacgt ttaaaatgat tacagcagca atcggtctcg
     2341 acaacggcac tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag
     2401 acagttcttg gggatctttt aaaacaacgc gtgtcagtag tgatgtatca caagtagact
     2461 taaaaactgc tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg
     2521 gtgagaaaaa atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc
     2581 caattagtat gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc
     2641 tagctgatac tggatatgga cagggcgaac ttctaattac tcctatccag caagcagcaa
     2701 tgtattctgt ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag
     2761 agacaaaaga taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc
     2821 tgagagaagt tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc
     2881 cgttggcagc gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag
     2941 agaacagttt cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt
     3001 tggaaaataa agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc
     3061 tcaaccaaaa ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta
     3121 tcgtcacagt ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt
     3181 tgatttactc acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata
     3241 agtgtactta ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga
     3301 ctttgctgtc caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca
     3361 ccgaccatca atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca
     3421 agcaatttgg tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta
     3481 aagtcagaaa aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca
     3541 tatcctaaag cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct
     3601 atcccagcaa aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac
     3661 attcctaaac aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct
     3721 gcaaatgaaa gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa
     3781 attagctgtt caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga
     3841 aaagataggc tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc
     3901 aaaattgttg atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa
     3961 atactgacag aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact
     4021 tggaatgctt tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca
     4081 acgaacatac caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct
     4141 aaaaacatta ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg
     4201 acccaattca atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct
     4261 gggataccta atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat
     4321 aaaataccaa taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc
     4381 ataacataca ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa
     4441 gatttttttg gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt
     4501 atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa
     4561 ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg
     4621 aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc
     4681 atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc
     4741 aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa
     4801 agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca
     4861 aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga
     4921 aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt
     4981 aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt
     5041 tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag
     5101 gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg
     5161 ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt
     5221 cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag
     5281 gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg
     5341 tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt
     5401 ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac
     5461 gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata
     5521 taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc
     5581 ttgtcaccat ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca
     5641 tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt
     5701 gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc
     5761 catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcta tctagccaat
     5821 ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct
     5881 ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt
     5941 tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga
     6001 tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag
     6061 ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc
     6121 ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt
     6181 tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg
     6241 ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg
     6301 aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt
     6361 gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg
     6421 tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg
     6481 ctgcatagat agctgcacta ctagctgctg gtccaccgcc aatcactaat acatcataag
     6541 gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt
     6601 cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca
     6661 tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa
     6721 tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac
     6781 aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac
     6841 tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag
     6901 aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg
     6961 gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag
     7021 acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca
     7081 aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt
     7141 tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt
     7201 tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta
     7261 catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg
     7321 cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag
     7381 ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac
     7441 cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag
     7501 taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt
     7561 attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga
     7621 agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa aattctcctt
     7681 gatgataagc atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac
     7741 ctccaataat aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg
     7801 gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga
     7861 ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg
     7921 ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt
     7981 atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc
     8041 ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca
     8101 gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc
     8161 acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt
     8221 ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc
     8281 ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac
     8341 atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc
     8401 ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt
     8461 ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga
     8521 ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag
     8581 aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa
     8641 aattttggtt gacatagcga tttttattgc ataaaattca cttgaataca aaaactaata
     8701 tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa
     8761 ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga
     8821 caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca
     8881 gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa
     8941 acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa
     9001 gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt
     9061 ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat
     9121 cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca
     9181 aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca
     9241 catacaatga gtattcttgc tgtgctactt tataaacgtt ttttatctgg agcttggccg
     9301 attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg
     9361 ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac
     9421 ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat
     9481 ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc
     9541 caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta
     9601 atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg
     9661 actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct
     9721 ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag
     9781 atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca
     9841 gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg
     9901 cgcttttcta atccaaatat tcctaatatg cctcagcgaa tcgccacgga tacctctcaa
     9961 aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac
    10021 ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc
    10081 attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac agaccttcag
    10141 cataagcttt ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg
    10201 gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc
    10261 agaaaaatag aagaaatgtt catcaagatg gtagcaggac caaatgccgt acgaaaaaca
    10321 ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt
    10381 acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag
    10441 ggattgtagg cacattgatg gatataccag taggagaggt atggcctagg gagaggatca
    10501 aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca
    10561 atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata
    10621 agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc
    10681 ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt
    10741 tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac
    10801 attccagcaa tgaatttgga ttgccgggat gggaacctga gcgcttagct cctatcaaat
    10861 ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt
    10921 ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag
    10981 atgatccgcc atactcgatt tttggattag ctcgaatcgt aaagaatcga gaagatttag
    11041 attggttgtg tcgcatagta gacagccctt ataacggaat cactctttgt acagggagta
    11101 ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgc caaagagatc
    11161 gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg
    11221 caccacataa atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg
    11281 acaacggatt taacggatac atccgtcctg atcatggaag aatgatctgg aatgagacag
    11341 gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat
    11401 gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt
    11461 attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa
    11521 gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt
    11581 gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt
    11641 ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag
    11701 gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt
    11761 gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga
    11821 tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat
    11881 catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg
    11941 gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga
    12001 cttctttcaa tacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt
    12061 gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa
    12121 tcttttcttt ttcaggtata aacaaatcaa atacagtaca aagggactcc accatttctc
    12181 ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg
    12241 tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt
    12301 gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagagatca ataaaaaagt
    12361 caatatattc atact
//
LOCUS       DACDHL010000072        12226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDHL010000072 DACDHL010000000
VERSION     DACDHL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.072"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..566
                     /locus_tag="IV228_002100"
     CDS             <1..566
                     /locus_tag="IV228_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8717978.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /locus_tag="IV228_002101"
     CDS             967..1572
                     /locus_tag="IV228_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8717979.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="IV228_002102"
     CDS             1588..2160
                     /locus_tag="IV228_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8717980.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="IV228_002103"
     CDS             complement(2412..2669)
                     /locus_tag="IV228_002103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717981.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="IV228_002104"
     CDS             complement(2795..3610)
                     /locus_tag="IV228_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717982.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="IV228_002105"
     CDS             4127..5452
                     /locus_tag="IV228_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8717983.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="IV228_002106"
     CDS             5445..5795
                     /locus_tag="IV228_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717984.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(6866..7141)
                     /locus_tag="IV228_002107"
     CDS             complement(6866..7141)
                     /locus_tag="IV228_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8717985.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="IV228_002108"
     CDS             complement(7113..8066)
                     /locus_tag="IV228_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8717986.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="IV228_002109"
     CDS             8678..10171
                     /locus_tag="IV228_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8717987.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="IV228_002110"
     CDS             10306..10602
                     /locus_tag="IV228_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8717988.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="IV228_002111"
     CDS             10703..11554
                     /locus_tag="IV228_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717989.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="IV228_002112"
     CDS             11575..11787
                     /locus_tag="IV228_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8717990.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4205 a         1873 c         2286 g         3862 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca cctggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaacggttc tgttgcaaag ttttaaatct actatcaaat
    12181 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatc
//
LOCUS       DACDHL010000073        11883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDHL010000073 DACDHL010000000
VERSION     DACDHL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.073"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            603..1574
                     /locus_tag="IV228_002113"
     CDS             603..1574
                     /locus_tag="IV228_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8717991.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1652..1888)
                     /locus_tag="IV228_002114"
     CDS             complement(1652..1888)
                     /locus_tag="IV228_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8717992.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1857..1988)
                     /locus_tag="IV228_002115"
     CDS             complement(1857..1988)
                     /locus_tag="IV228_002115"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8717993.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2064..2447)
                     /locus_tag="IV228_002116"
     CDS             complement(2064..2447)
                     /locus_tag="IV228_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8717994.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2480..3265)
                     /locus_tag="IV228_002117"
     CDS             complement(2480..3265)
                     /locus_tag="IV228_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP8717995.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3443..4543
                     /locus_tag="IV228_002118"
     CDS             3443..4543
                     /locus_tag="IV228_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8717996.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4533..5357
                     /locus_tag="IV228_002119"
     CDS             4533..5357
                     /locus_tag="IV228_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP8717997.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5363..6169
                     /locus_tag="IV228_002120"
     CDS             5363..6169
                     /locus_tag="IV228_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8717998.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6166..6996
                     /locus_tag="IV228_002121"
     CDS             6166..6996
                     /locus_tag="IV228_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8717999.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7003..8034
                     /locus_tag="IV228_002122"
     CDS             7003..8034
                     /locus_tag="IV228_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718000.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8141..9414)
                     /locus_tag="IV228_002123"
                     /pseudo
     CDS             complement(8141..9414)
                     /locus_tag="IV228_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9641..9826
                     /locus_tag="IV228_002124"
     CDS             9641..9826
                     /locus_tag="IV228_002124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718001.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9950..10666
                     /locus_tag="IV228_002125"
     CDS             9950..10666
                     /locus_tag="IV228_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8718002.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10568..11088)
                     /locus_tag="IV228_002126"
                     /pseudo
     CDS             complement(10568..11088)
                     /locus_tag="IV228_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11376..>11883
                     /locus_tag="IV228_002127"
     CDS             11376..>11883
                     /locus_tag="IV228_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8718003.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRM"
BASE COUNT         3406 a         2203 c         2715 g         3559 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tggataacgt gattttttgc
       61 tgtaatgaca aggttttagc tgtttcagat accatgtatc caaaaatagt ttattgagaa
      121 aataggagag tatttgatac taactagtag gaaaaaatga gataaaaagt aaatggaatc
      181 atttaaggta atctgaaata aaaaatatac agccctgtgg tactttacct acaaggctgt
      241 atatattttt tactctcagc gtttttgact ccgtgtctga ctccgtttta ttgattttga
      301 atgttattca atgaccattt aacaggtcaa atttgatgtt tttgattccg tatgacgccg
      361 tttgatatct agtgatatag tgatttgagt ctacatcgaa taagggaata aaccaataaa
      421 aaatgattta gaatattaaa ataacaaaaa agaggactac ctgtattagt cctctttttt
      481 gaccagattt tgaccagtct attttacatc ctagtatatt tatatgagct aattttatga
      541 ttaaatggta agtttttcct tgtgtgtttt agtttaaact agtgtgtaat tcaaaaatct
      601 atatggaagg aacagtatta agaatagaac agggttctct acatgatggt gctggtttac
      661 ggaccgttgt ttatttaaag ggctgcccat tacgctgtgc ctggtgttcg attcccgaat
      721 cacaaagcaa gcagattgaa aaaggatttg gccagaccat gaccgctgaa gaggtaatgg
      781 atgagattga gaaggatgct gttttttact ttcactcgga tggcggagtg accattagcg
      841 gaggcgaggc actggttcag gcggattttg ctaaagaaat tttgcaaaaa tcgaaataca
      901 ttggaatcaa taccgtactg gaaaccagct tttgtggtgc ttataatgaa atccaaaagg
      961 tagctcccta tgtggataca ttgtttgtgg atgtaaaaat gtttaccagc aagctccaca
     1021 aacaatggac cggcttagat aaccaacaaa ttttgaagaa tattcgtcgc tttttaattg
     1081 agtatcctaa ctgcgaagtg cgaattcgag ttcctgtggt tccaggtatc aatatgaacc
     1141 tgaccgagct gctgacgatt gcttgttttg tggcagatct tgatcggttc gtaccgttag
     1201 aattactacc gtaccactgc tatggtatgc acggttacca agcattggga ctggagtatc
     1261 cgttggcaga cacgccagcg ccttcaccta aagaaatgtt tgcattggca gatcaactgg
     1321 ctcgcacggt tcctcatttg ctggtcacag ctttaaccaa aacatttatt tatttattga
     1381 atccaagctt tatcttatgg ataaatgaaa gacactcact agctgcagcc agctcaagaa
     1441 tagagctttt gctgaagtca gtgagtgtct ttttacgatg ttttttcgaa gttaatttgt
     1501 cgaatgaaca ctggctcaat cggtacatct tcactgtttg tcggctgagc tgcgatcgct
     1561 gctacgattt ctaacccttg gtatacctgt ccaaaaacag tataacgacc atccagctcc
     1621 ggtagtcctc caagctccaa gtatgcttgc tttacattag caggtcagcc tgcgcctttg
     1681 tgttttgcac aataaaaaac tggttgccgt tggtgtgggg gccgtggtcc cctaaggcca
     1741 aggcgccatt aaaatgataa agtccgcgat gcacttcatc agcgaaaaag ccctcatagc
     1801 tggaaacagc ttcatcacca gaaccatcca aagcgccacc ttgaataaca aattccttaa
     1861 tgacacgagc cgaaaggtgt gttgtcataa acgccttgct cagctaggga gcgccagttg
     1921 gctacagcca ggggagcttg gtcaggaaat aagcggagac gaatttcacc agcatcggtg
     1981 accagcacac aaacttcttc ctgctgcggt ttttctaatt gaaagagagg aaggtttgca
     2041 actgaaacgc cttgatcctg ccatcaaata tcgaaaatcc gccactcgac cttcgcctga
     2101 tcaccaaggc gagccattcc caataaaacg gtataggcgg tttcatccgc ttgaaaggtc
     2161 aattgcagct gtcctcgtaa atcgttgtag tcatctgggg cggctacttg ataaaaaaga
     2221 tgaaactgcg catgcaaata ttccaaaggc tgatctttaa aggctgcaaa ataagcctga
     2281 actttttccc gctgcaagct gtcagccacc aaaaaatttt ccagttgttg ttttggaacg
     2341 gctgctaaca aggatcggca aaccgcctca attttcaacg catcccaatg tcgcgcaatt
     2401 aaataatgga tgggtgcctc ttgattgtag tcccacaggt tgctcatttt tttcaccact
     2461 ttcttctatt atataggaat cacaaaatcg gcagcggcgt caggcggcgt tcttttcctt
     2521 gaatgactag caacttttct acctgacagt ctgccaacag ctgactcatt tttttaaagc
     2581 ccagctgata atcttcggca acatgagcat ccgagcctaa ggtaaagagc tttccgccga
     2641 cactttgata caaaggaacg gcataacggt aaaggtcttc gttgccgtat ttcaagaagc
     2701 tcttggcatt taactccatc gccaattctc gttcaacaac ctttttgaaa atcgttagca
     2761 gctgagcctc aaaatgctcc gcgaattctt ccgctgtgaa ggtgaagcgg cgtaatccat
     2821 aatcaaagtg agtcaaaata tctccttctg gaaaatccgt caagacctgc aacatctgat
     2881 caaaatattc cttggtagtt tgaatgggat ctttggttaa aaccacatcg tccatataat
     2941 caaagcggcc attttgatga atacttacca gctttaaatc ataaggatgc tgagcgaggt
     3001 aatccacgat ccgctcttct tgtcctggaa caacgccaat ctcgatacct cgcagaaatt
     3061 tggtggaata ggtttctttt aaagagtcca gtttgtttat ataggctgga taatccggaa
     3121 tgtcatcttt tccggtagat ggattgttta aatcaaagtg atccgtagaa acgaaaaact
     3181 ccggctgata ggctaaatag ttttcaaatt tttcctctga atcaaaggaa aaatacgtgt
     3241 ggagatgctg atcataatac tgcataggga gcctcctttt ttctactatc ataccgcaaa
     3301 agctttagaa ggttaacggg aattcgtcga aatttacaaa ccatatacaa cctttacatt
     3361 gttcatgaaa ccttttcaag aaactgattc tctcattaca aagctttgtt agaatggaat
     3421 tatcgaaagg ggagaagaat gaatgaaaat gaatgaaaaa attcgtaaca tgactatcgg
     3481 tttattttgt gcagcaacag taagtatcct gctgacaggc tgcggctcag gtgaagcaac
     3541 gaagcaatcg gctgcggcag acgactcttt agatacgatt atagaaaaag ccaaggaaga
     3601 aggagagatt gccagcttgg gaatgccgga tacttgggca aattgggtag gcacttggaa
     3661 cgaccttgaa aaagaatacg gcttaagtca cgtggatacg gatatgtcca gtgcagaaga
     3721 attggctaag tttgaatcag aaggaaaaaa tggaaccgcc gatattggtg atgtcggtat
     3781 cagcttcggt ccgttggctg aaagcaaaga gttaacctta ccttataaga ccaactattg
     3841 ggaggaaatt ccggattggg caaaggatga tgatggggat tggttgttaa gctatacggg
     3901 aaccatttcc tttatcaccg ataaagagaa tgtgaagcaa gcaccgacta gctggaagga
     3961 gctgcaagag ggtgactatc aagttagcgt gggggatgtg acgattgcca accaagcaca
     4021 atttgctgta ttggctgcgg cgattgccaa cggaggtgac gaaaaggata ttcagccagg
     4081 tctgaattac tacaaaaaaa tggcagaaaa aggccgtttg tccaccgttg atgccagcgt
     4141 agcaaatctt gaaaaaggtg aaatcgatgt ggcagttgtt tgggacttca atggactaaa
     4201 ttatcgtgac caaattgact ccgagcgttt tgaggtaact gtgccaacag acggctccat
     4261 cattagcggg tatacaacct taatcaataa aaatgcccct catcccaatg cggcaaagct
     4321 agcacgggaa tatattttat ctgatgaagg gcaaatcaat ttggcaaagg gctatgcgcg
     4381 acctattcgc aaggtagagc tgcctaaaga agtagctgaa aaattactgc cggaggatgc
     4441 ttatgaagcg gcggtgccag ttgaagacaa tgaagcttgg aacaagacct ctgtagagct
     4501 tccagagctt tggcaggcgg aggtactgac ctatgcaaag tagacttgac aaactgattg
     4561 tagttatact agacggctgt cgctacgaca cagccgtcgc ccagcttggg ttcatgaatc
     4621 atttagttga gcaccagcaa gcaagcttat tgaaggtaaa aagtgagctg cccagcaatt
     4681 cacgtccgtt atacgaagta ctgatgaccg gcgttccagc ctatgaaaat ggcatctata
     4741 gtaattacca tgctcagcga tccaaagagt ggtcgctgtt tgaattagtt aaggaagctg
     4801 gcggaaaaac aggagccgcc gcttaccact ggtacagcga gttgtacaat caagccccct
     4861 ataatcgttt ggttgatcgc atccagcatc aggaggagcg actgattcag catggcatct
     4921 tttatagtga ggacacttac cctgattcgc atttatttgc ggatgcccat tatttagtga
     4981 atcagtatca gccagacttt ttggtcgtac attcgatgaa tattgatgat gtcggtcaca
     5041 aatttaccgc cgattctcat gaatatgcag cagcggttaa tcaggcggac agattattgg
     5101 ctatttacct tcctgaatgg ctgcaaaaag gttatcaagt cgtggtgact gcggatcacg
     5161 ggatggatgc gcacggactg catggcggca gcttggctgc acatcgtgaa gttccttttt
     5221 atttgatttc aaataaacgc caaaagctga aggaacaaac tgtaccacag ctgctggcgg
     5281 ctccattgtt ttgctggtta ttaggaattg caccgtcaga gaaaatgcag gacattcaag
     5341 aattgatgag gggatgaaag agatgaaaaa acgcttggtt ctggttcctg cctcctttat
     5401 cttctgtgca tttttactgc tgccgctgct ttttatggtt ctgtgcagct tgcacagtga
     5461 tttaactaac cagtggacat tggccaatta cagtgatatt ttcagcaaca aatattacac
     5521 acaggccttt gttaacagcg tactgattgc tgtcagctcc agtgtgattg ggttaatcgg
     5581 gacgtttatt ttatgtttat gcttaatgaa tctatcgact aaattgcagg agaaaatcac
     5641 ctttgtttcc aatttggcag ctaattttgc tgggattccg ctggcattct cttttattat
     5701 tttgttgggg aatgtaggtg tgctgaaaat gattttgcca tttctagcgg acttcaattt
     5761 gtattcttgg tggggattga ccatcacgta tacctatttc caaattccgt taggggtctt
     5821 gtttttgtat cccagcatga aggaagtgaa aaaggaatgg ctggagtccg cacaattgct
     5881 gggcgcttca tcagcgtatg cttggttcaa ggtggctttg ccttttttgc gaccatcgat
     5941 tgccagcacc tttgtcatct tatttgctaa tggcatgggg acctatgaaa cagcgtatgc
     6001 cctgaccagc agtaatgtaa atcttttaac gattcgaatt gcggctttag tgtcgggaga
     6061 tgtgttcgcc aagcctaatt taggcagtgc gttggcagtc gctttaggtg tcctgctggt
     6121 aacggcaatg ttgattattc aaaagaaagg caaggtggtg cagacgtgaa aaagttaata
     6181 aaaccgattt tgttctgctt tgtcctgtat tacttgctgc cgattattgg aacgattttg
     6241 tatgccagct ccactaaatg gtcaaaatcg cttttaccta gtgattttac cttgcaatgg
     6301 ttccaacagc tgctgacgga cagggaattt attgctgcgg tgggtcgctc acttttatta
     6361 gcaggtgtgg tcttggtgac gattctgttg ttgatgattc ccaccattat ttggattcat
     6421 ttgtatttcc ctcgtttgaa tcgttggctg gaaaagctgc tcctgctgcc gtatgcttta
     6481 ccaggagtga ttttggtaac ggctttgctt cgaacgtatg ctgaaactgg tatcccaatg
     6541 tttgtagtgt tggtaggtgc attgtttatt acggcgttgc cgattgtcta tcttagtctt
     6601 aacaaccaga tgcgcttaat taatctgaag gaattagtgg atgcagcaga aaccttgggc
     6661 gccccgatgt ctacgattat tatccaggtg ctgtttccaa acatccgtat cggtgtgacc
     6721 ctagtttctc tgatgatttt ttcttcggtt tttggggagt acatgctgac gaacctgttg
     6781 attggcggtc gatttcagac ggtacgcatc tatatgctgc ggcggatgaa cgaaaacggc
     6841 catttggcta gcgctgtgat ggttttgtat ttgctgttta tggtgggaat tgctttaact
     6901 acctttatgc tgacttatcg acaaaagcaa cagctgtccc ttaagcagac gagggaagag
     6961 aagcagtcga aaaagaagcc cttaaaggag ttgtgacgga aaatgttttt agaaatacat
     7021 gatctcaatc tgttttttga tgaaaaacag attttagcta ttgatgaaat tcagattgaa
     7081 aagggtgagc tggttacttt actaggtccc agcgggtgcg gaaagtctac gttgctgcgc
     7141 tgtatcacgg gattggaaca accaaagagc ggtgagattg tgctggacaa tgagaatatt
     7201 gccgataagc ctacgaagga tcgcaatatc ggctttgttt ttcaacagta tgcattgttt
     7261 ccaactatga ctgtttttga aaatgtagcc tttggtttga aggtaaagaa gctgagtcct
     7321 gaggtgattc aggaaaaggt ttttgagatg ctatcattgg tggatatgac ggagcaggca
     7381 gataaaaatg tccagtttct ttctggcggt caacgacaac gggtggcact ggctcgctca
     7441 ttggtcactg aaccgaaagt cctgctgctg gatgagccat taagtgcttt ggatgcccga
     7501 attagaaaac agctgcagcg ggatttgcgc gctatccagc aatcgctagg gatgacgatg
     7561 atttttgtga ctcatgacca agaagaggcg atgcgaatca gtgatcggat ttttgtgatg
     7621 gaagctgggc gggtggctca ggtttccacc tcgaaggaat tgtatcagaa tcctcagagc
     7681 cgatttgttg cggaatttat cggcaactat aatcgtttcg aatggtatga gctgaatcag
     7741 tatacccacg agttttccgc taagggcaat cgctgcattt attatttaag accggagctg
     7801 attcaatttg aaccggttcc tcagggggtt aagattccgg ttctatggaa ggaatcattt
     7861 attttaggca acattcaacg gtatgttttt caaacgctgg agggcaaaga aattttagtg
     7921 gatcggttga acgatcggga agagaaaatt agcgatgcct tatacattca gctgaaagat
     7981 attctagcaa tccctgtgga aattgatact cagcattttg cacaacaaag ctagtgtata
     8041 gtcggcaaac atttctggct ctttgtcaaa taggactgat agagtgatat aagaacatct
     8101 aaggtagaat tgattttctg ctttagatgt ttttttatgt ttaataagtg atcagatgat
     8161 tgattaagaa aattctggtt ctcatcctaa cccgtaagct attcgtttca atgctttgat
     8221 gtgggtatgc agattttcta ttttgccatt tgagtaaggc aactctaagg ctaaacgaat
     8281 gccggattca tagtttataa acgctttttt tatatgatgc agttctcttg gaaccgactt
     8341 gggcattgtt tttacaagct gaaagaagat gtccgcttct ttgtcataaa aagcttgaag
     8401 aatttcttga tagtaagcat aagctattcg tagttcatca gaagtagaaa gtagacgttc
     8461 tacgacttca ctttcggtta aataggccca accaaaatta cgccattttg tccatttatc
     8521 ataaacaagc gtgtcctgtc gtttgatcag aagtcgagat aaagccttca cttgtcgata
     8581 gtgtttggct tgtattggat cgtttcggtc gaagcttttc atgactcata gtcaaatatc
     8641 cttaaaactg cgaatgaggt gcttgacgat gtgaaagcga tccacaacga gtttggcatg
     8701 aggaaagcac tcttttatca gcaaagcata agaggcgttc atatctgtta cgagtatttt
     8761 tacgtttaaa cgtgcacgtc gttcaaattg cataaaataa gaaatgagat ctttcttctt
     8821 acgtgaagga agaatatcca gtaatttccc agtatcgcca tccatacaag aaaaagaata
     8881 gggaccgact tgatttttca atgaacgaaa ttcatctacc aatgaacagc gaggcaacca
     8941 atttattttc acgtttgtca tggggagcag agaagcgagt gttcgcgaaa cggttgacca
     9001 agaaacgccg caatgtttag aaatcgtcgt cattgcttgt ttttcagtca attcctccaa
     9061 tattttataa tgtacttgtt tggcgataaa acagtttttt tcaacaatgt aggtattcgc
     9121 cgtaaaatag gtgtgacagc cacgacagag gtaccgttgc tttgccaaag caagataagt
     9181 ccgttgattc gcacacttat tgagaaggac cagcgatttt ttctttccgt tcttaacgat
     9241 cagcccatgt tgattcacct gtttacactg aggacagggc tgaatcgttt taatgagcgt
     9301 ggcagaaaca acgatactgt tgattgaatg tatttttttc ttctcgacat gagtgatttg
     9361 aatgtttcca tctttgattc ctagtaatag tttgataaga tgaagatcag gcatacagaa
     9421 cgctcctttg attgtgttgt ggtgattcaa ttctaacgga gcttctgtat gttttgtttt
     9481 tttatgctca aaaggactga tagaatgatt tactatcagt cctagaaatt atagcgccac
     9541 atttctttca cctttgactt ttcagacggc agtttttatg ctgatactgt agagagagca
     9601 ggacacagct tttgcttgtt ttgggtaagg gggaaagaag atgaccaaag cacttatttt
     9661 tgattttgat gggacgatga ttgacagtga atggatgttt tacgagagta ttcatcacta
     9721 tttactgaag cctatttact ggagcagttc ggctataaaa ttccttttgc agactatcaa
     9781 caaggtgttg ggacggcagc agaactgtcc ttttatgaag ggataagtca agcggttggg
     9841 agaccagtgg atgctgaaca ttttgagcag cggatcagca gcactggaaa atcggagtag
     9901 tctcaattct gaactgcggg agctagacta tttttttgaa gcacatccga tgattaagga
     9961 aaaaattgat tcctttgtga cgatcgatga tcttcaggtc ggcaaaccag atccagaagg
    10021 ttatttgttg tgcttgaaac ggctgagggt tgaacaggct gttgtagttg aggatagtcc
    10081 agtcggtgcc caagcagcac cgcatatgac agttgatgta caagcgttgg atgcagattt
    10141 tttcgccttt agcgggcata aaatgtgcgg gccgacagga atcggtgtat tatatggcaa
    10201 acgtcaattg ttagaacaaa tggagccagt cgaattcggc ggggaaatga tagattttgt
    10261 caatctttac gatagttcat ggaaagagct gccatggaaa tttgaagctg gtactccgaa
    10321 cattgcagga gcaatcgatt acttgaatga gattggtatg gagaatattc atcgttatga
    10381 gcaagaactg gttgcctacg ttttacctaa attacaagcg atcggcggta tcaccattta
    10441 cggaccgcaa gatcctgaac atcatactgg cgtggttgct tttaatttgg atggcatcca
    10501 tccccgtgat gtggcgactg ctctggatat ggaaggtgtc gctgtacgtt caggacacca
    10561 ttgtgcacaa cggttcatcg acgatttctg tttgactaat tggtcgttta aacagtcgat
    10621 gataatgccg tttgagcggc tccaatgtcg tggaatcttt gagtaagagt ttccaatatc
    10681 gcttgagccg acggtacttc ttctgatttt cagattacgt gattgatttt cgtacagttc
    10741 tatgagaatc gcctgcttca gttcctttgt tagttgatga tgttcatcaa ctaagtccgt
    10801 tttcgcagaa aaggtctgcc cacaatcata gcatagaaaa cgggtacgtt ttaattctaa
    10861 aatagtcaac ttttctttga ctttcaacat ctgagtacgt gtcgtatagg aaccgttgcg
    10921 gataatacga atcgatccac agtgttcaca atgggaaggt gagtcatcca atctcccttt
    10981 aataaactgc gcttgaacgc ctttatattc tttttcaatc agccattctt catcaaaaaa
    11041 taaatgttta tctgttaatc cgagtaattt tctagtatga ttatccatag agcgactcct
    11101 ttggtagggt gatgttttgg tcgactaaat tttaccaaaa gtgagtctct ttttgagttg
    11161 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaacaaca caaaatatta
    11221 tagaagcctt ttttctaaag gatttcaagg tgtctgtttg catttattaa aaaaaagaaa
    11281 aagtattggc aacagaaggc gtcaccaaca ctagatatta tagagccata atatgctgta
    11341 taatatctag tgtataggct taagtggagg tatatatgaa aatattaact gttgaagatg
    11401 ataatatgat aagagaggga ataagtgaat atctttcaga atttggatat actgttattc
    11461 aagctaaaga tggaagagaa gccttgtcaa aatttaatag cgatataaat ttggttatct
    11521 tagacattca gatacctttt ataaatggtt tagaagtgtt gaaagaaatt agaaagaaaa
    11581 gcaatttacc aattctaatc ttgactgcat ttagtgatga agaatataaa attgatgcat
    11641 ttactaattt agtagacgga tatgtagaaa agccattttc attgccggtc ttaaaagcta
    11701 gaatagattc tttaattaag aagaattatg gacatttaga gaagtttgaa tataaggatc
    11761 tatcggttaa ttttaatagc tatacagcta aaataaatgg tgaagaaata gatgtaaatg
    11821 caaaagaact agaagtatta aaatgtttat tggataataa tggacaagtg ttaacaagaa
    11881 tgc
//
LOCUS       DACDHL010000074        11878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDHL010000074 DACDHL010000000
VERSION     DACDHL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.074"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(64..270)
                     /locus_tag="IV228_002128"
     CDS             complement(64..270)
                     /locus_tag="IV228_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718004.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(286..900)
                     /locus_tag="IV228_002129"
     CDS             complement(286..900)
                     /locus_tag="IV228_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8718005.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1047..1730)
                     /locus_tag="IV228_002130"
     CDS             complement(1047..1730)
                     /locus_tag="IV228_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8718006.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1839..2297)
                     /locus_tag="IV228_002131"
     CDS             complement(1839..2297)
                     /locus_tag="IV228_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8718007.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2381..4144)
                     /locus_tag="IV228_002132"
     CDS             complement(2381..4144)
                     /locus_tag="IV228_002132"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8718008.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4365..5825)
                     /locus_tag="IV228_002133"
     CDS             complement(4365..5825)
                     /locus_tag="IV228_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718009.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6033..6959)
                     /gene="glsA"
                     /locus_tag="IV228_002134"
     CDS             complement(6033..6959)
                     /gene="glsA"
                     /locus_tag="IV228_002134"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8718010.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6982..8409)
                     /locus_tag="IV228_002135"
     CDS             complement(6982..8409)
                     /locus_tag="IV228_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8718011.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8440..9654)
                     /locus_tag="IV228_002136"
     CDS             complement(8440..9654)
                     /locus_tag="IV228_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8718012.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9995..10237)
                     /locus_tag="IV228_002137"
     CDS             complement(9995..10237)
                     /locus_tag="IV228_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8718013.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10269..10826)
                     /locus_tag="IV228_002138"
     CDS             complement(10269..10826)
                     /locus_tag="IV228_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8718014.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10839..11027)
                     /locus_tag="IV228_002139"
     CDS             complement(10839..11027)
                     /locus_tag="IV228_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8718015.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(11040..11606)
                     /gene="amaP"
                     /locus_tag="IV228_002140"
     CDS             complement(11040..11606)
                     /gene="amaP"
                     /locus_tag="IV228_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8718016.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3479 a         2451 c         1941 g         4007 t
ORIGIN      
        1 ctttgataat tttttagttt tatgtgtttt tttatatgtt ttttcacttt tcggaataaa
       61 tatttatttg ttaaagtaat taaacaatcc gctaatcgtt tgatctggca tttctatttc
      121 cttaccatct aaagtaacag tagtaacgac aaaatggtct tgcaatggcg gaacttctaa
      181 gtactgccct tgcagaaaat cattcactgt ttcatattct tttttcattt cttttgaatc
      241 tgtcgatggt ttatagctta tttttaacat aactagtgct cctttttatt cgggacgtgc
      301 aatcgtgaaa atttctccta ttgctgcata atcatttcct gccaaacggc ttacagcatt
      361 caattttctt ggatcgatcc ttcctgtctt atcatcatag actgtttcat ttaaatgata
      421 tttctgtact tctagcaaaa ataaatcagc tgttacttta tttttattcc gtatctcgac
      481 atgttcatat aaggatgttt caaaacgaac ttttgcttct gtcaatgctg gtactgaaat
      541 gattactgaa tcagttaaag tcaagtcagt caggtacagt tcgctttcct cattaggcag
      601 attggctgct gttttattta cttcttctac gttgtcctca tcaaggatat ggataacagc
      661 ttctcttgtc tcaagaatat tctttgctgt gtcttttact gcatcttgtt ttctctggat
      721 cgccaaggat aagatgggtc gattcgaact gacgatattg aagtaactga agggtgcgat
      781 attcactata tcttttgctg atttagtcgt tactactgct actggtctag ggatgactga
      841 accaatcagc aacttgtaat tttcacgttc tgtcaattct gaaggactcc gttcaaacat
      901 ggtttcgcct tctttctata aaattcgtta aaaagcagat cttgaagaca aaatgaaaag
      961 taatgctttt ttcatcttct tagaatctgc tttaaactat ctatcaattt ttcttttttg
     1021 tcaagaatct gtgccgtgcg tgttttttag ttatttgctt tacttgcggc ttcgacatat
     1081 cttttagtga tcaattgcgg acgggtgata atagatccaa ctacgactga aaaagcacct
     1141 aattcatatg ctttctctac ttgttcagga gagtcagtat gtccttccat aattactggg
     1201 atatcgactt gttctatgat ttgattcaat aaatcaaaat caggtgtctg cgtctttcgt
     1261 gaatattctg tatagcctgc taaagtcgta gacaaaagat caaaaccatc ttcttgcgct
     1321 tgaagcgctt cctcaaaagt cgaaatatct gccatagcta accgaccgtg ttcgtgaatc
     1381 atttggacaa gttcagcagt tgttttttct gtcggacgtt tacgagaagt tgcatccagt
     1441 gcaatgattt ctgcttgcgt ctccaaaagt tcttccactt ctttttctgt cgcagtaata
     1501 aacacttctg acccatcgta atctcttttg ataatcccta ttaccggtaa atcaacgatt
     1561 cgcttgattt cattgatatc ttctgtcgta tttgctcgaa taccaactgc tccgccttct
     1621 tttgcagcca aagccattct ccccataata aaagaactgt gtagcggctc gttttccaaa
     1681 gcttgacaag aaacgacgac tccgcctttg atttgttcca gtgtatccac aatatctcct
     1741 ccagtaatgt tatataaaaa tacagcttca tataaaaatg taaccctttt ctattcagga
     1801 gacaataatg agctatttct ttcagcagac ctttcttact aaaagaaaat tacttcaaat
     1861 tcttcgtata cgaccagctt ctcttctgta aattctatgt tcaacgcttc tttataagca
     1921 tgtttaaaaa actcagcatg gactttccgc caataagcta atgtccgatc cccttctcct
     1981 tctttccaag catgctcttt cgtgacttca ctaaaaggca agatggtcac atttatattt
     2041 ttgatgatac aaactggttc attttttcca ttcaggacca cgtctaattg accaactttt
     2101 ggtaaaggct cattctcttg tttatacaga tcgtaaccac ttgatgtacc agtctttatc
     2161 ttttttacaa ctagttcgct caactcgtct gccatttttt ctgtatcccc aaatgaaaag
     2221 aaacctaatt tttctgattg ataactagga tatttttctt ggaacttttt gacatattct
     2281 tctacttgct gatacataac atccacccct ttgcttttta taatgaaaaa accatccaga
     2341 tactagtata aacaattatc tggatggtga gcataatgaa ttattgaata ttcaagacac
     2401 gcagactatc ttcaacgatt tgttttggtg ttaaatgata acgttcataa agctgcccta
     2461 caggaacgct atccgtgaat tctttctctg caccaaaatt taatacgttc attgaagaat
     2521 caccataata acgactgata ttttcgccaa atcctcctga caaactacca tcttctaatg
     2581 tgatgactaa ttcgtgtgtt tcttttaatt tatctaatgt ttctacgtca ttttctgtga
     2641 tcgttcctgg attgatcaac gttgcttcga ttcctttttt tctaaaagct tttactgttt
     2701 cttttcctaa ttcaagaaaa ccgcctaaag ctaaaattgc tacttttcct ccattattta
     2761 ctgtttgata ggctggaata ctataatcat ttgcgatagc tgtaccatgt tctacaccat
     2821 gtgtaggaat tcggattgct acaggctgcg ctgtttgttc gaacgcccaa tctagcatag
     2881 agatcatatc ctcttttgtt gctggagcaa gatatttgat gttcgggata cttgataagt
     2941 aaggaatgtc aaatgttcct tggtgtgttc ggtcactagc tgagatcgtt ccgccaccga
     3001 ctaaaataat cactggattc tcgttgatcg ctaaatcatg tgataattga tcatatgccc
     3061 gttgcaagaa agtacttgat tggaagacga ctggacgtac tccctgagaa gccaaagcag
     3121 aagcgaaagt gatagagtgc tgttcagcga tccccacgtc aaaataatga tctggatgtt
     3181 tttcttggaa tgttttcagt ccaaaggcgc ctggtatcgc tgcattgact gcggcaatag
     3241 gagctgtact tgtttgcatt ttcttatcca aataatccat gatgatcata ttaaatgttt
     3301 ctccagaagt tacttgcttt gcctccccgg tggacagatc aaatggtaca tgccaatgat
     3361 atttcatttt attatcgatt gccggctgat aaccgcgacc tttttccgta tggacgtgaa
     3421 gtacaatcgg atgatcaata tcttttactt cttcaaataa acggatcacc gtttccagat
     3481 cgtttccatt atccacgtaa cgataatcaa atcccattgc tttaaatagg ttattgctgc
     3541 ttttaccgtt ggtttgacga agctcagcta agtttttata taaactgcca tgattctcat
     3601 caatagacat ctcattatcg tttactaata cgattagatt ggattgtaaa acaccaccat
     3661 tgttcaatcc ttcaaaagct aagccaccac ttagggagcc gtcaccgata aatgctacca
     3721 catttcctga ttctttcttc agatctctcg ctttagctaa acctgttgct aagctgatag
     3781 atgtagatgt gtgccccact gtaaaatagt catgtttact ttcatgttgg ttggaatatc
     3841 cgctcacttc acggtaatgt cctttttcaa aaccattttt tcgaccagtt aaaagcttat
     3901 gaccataaga ctgatgggaa acatcccaaa cgattttatc ctttggtgaa tcaaatacat
     3961 aatgaaacgc aatcgttaat tctactacac ctagattcgg accaacgtgc ccacctacat
     4021 cgctgtcctt ttgcaagatt ttttggcgca tctcttctgc caactgtttc atttcctcaa
     4081 cagataattt tttgacatct gcggggctat tgattgcatc caatacatgt aatactgtac
     4141 tcataataca ttctctcctc cgtactatct tcttttaatg aaaatagatt tgttacactt
     4201 tatctattct actacttaga gttgactcta agtcaatagc attttaaaaa aatttcagag
     4261 aaagcttgtt atataagtag atcttcgcac gaatcaggac tcaaataaaa accttctaca
     4321 gacaattaaa aagctttgta gaaggtctcc taattaaggt ctcatcaaga aatatgtttt
     4381 tttaataggt gaagttcctg agcaatatga tacaaatgga tgtttgcacc ttttaatgag
     4441 attggataga tctttgtctt ttcagtttct aatccatttt gatagctagc agcatcgcac
     4501 aaaaccactc tttttttcgc acatagacat tcatcaggca attcttctaa tgaacgaaat
     4561 aaaataggat ttatttcagc gatgccttct tccgcataca aatgaaattg ggttcgataa
     4621 aaaaattgtt tgactgaact ttctgaaaca acaaaaattt ctatttcctg tctccccgct
     4681 tgttcactgt tgatcaaaga aagacttttg acgaattttc gaatgaaatt ttcattccat
     4741 cttagttgga tttgattttt ctttttccac tcttccaata tttctacaat ttttccgtac
     4801 agaagttgtt ctttttctga tagaagatag atcctttccg gtaaaaatac ttgtccatct
     4861 gcccacaacg tacggacaaa ttgtaaaaag gattgcttga aaataaaatt ttctgcatcg
     4921 tcgatttcag cgatactaat aatctgtaaa atcaattcat tcagtgatag gtcattgaga
     4981 acaaaatttt tataaacaac ttcgaaatct tttcttaaca gctccatgtt ctcattggta
     5041 taatttctac tgttgaacaa agaaaaaata taatagacat cttcttcttg taagtctaga
     5101 attccttttt gctcttccaa tccttgacag actaattgaa ataacggctt cgtttctgcg
     5161 cttttcttat cttcttcttt cagtccaagg ggaaatgcct ttcttccgat tgcgatctct
     5221 attcctcgta tgataaagga cttttcatct tccgaatatc ttcttttaaa aaaatgagtc
     5281 tctacataat caatcaactc gttacaagct tcttctacat acggattttt attttcatag
     5341 ccttcccaat tgacataacg tactaaagct agcaagatgt tccgcgagtc aaattctggg
     5401 ataatgattt ctttattttt caaataatca ttttctttaa aaaaatccaa tacgattttt
     5461 ttgattgtat atactttgct caaagaaata aattcaagat cagccagttc tgtactcgtg
     5521 aatataccat taaaataata acttaaaaac tttaaaaaat aagattgacc gtacagaaac
     5581 tgtgtgagct cagaaagagc aataccatgt ttgaatacga gtttatactt atgcttctct
     5641 cttacttgtt cttttataca atattctaga cactcggcta gactttctag atcgtgaagg
     5701 atcgttggtg tagtcaccct tagagaaata gctgtttgtt cgatgtctat ttcttggaat
     5761 tcaaataatt gttcacacag atacactttt ctaaatatat ttcgctcgat gtaattttcc
     5821 agcattcctg atacacgcct ccagtttttc ttatgatttt atttaatcat atctgtaaat
     5881 cgtatttttg tttccttttt atttagaaaa gccctttcaa aaaccaataa aaaaagaaac
     5941 tgggacaaaa gtcaaacaag ctcacaattt cagcctcatt gtaggtagct ttgagccgtt
     6001 tggactttta ccccagcctc tttcaagaat ggttattcaa agatgtcagc gtctagttct
     6061 ttgactacat ctttcaacaa ttggatacca gctgcgctgt taccaaatgg atctagtgcc
     6121 gggctgaaga cacctatgcc gtaacgattt ggcacagctg ccatcaaccc gccgcctaca
     6181 ccgctttttg caggtacacc aacatggacg gaaaactctc ctgattcatc atacaaacca
     6241 gcagttgtca taattgattt gacgattgta gcactttctt cagtaatgat ttgtttgcca
     6301 ttccaaggag caattccttt atttgccagc acagcagcta attttgctat gcctgtagcg
     6361 gtgacattga gggagcattg tctgaaataa gcatcgagta tctccggtac gtctccttcg
     6421 atcatttgat tacctttcat atagtaagca agagagcgat tgatatcacc tgttcttgat
     6481 tcagaatgat agatttcttc attcaatgtg atattaggat catcacagat ctctttcata
     6541 aatgacaaga ttcgctcaaa tttttcatct gcatctttac cggcaatcaa cgaggttgtt
     6601 gcaatcgctc ctgcattaac aaaaggattc attggatgtt tgcgatgatt gatttccata
     6661 ttcatgatag aattgaaggc aaaaccggtt ggttccgtgt tgattgtttg aaagactttt
     6721 tctattccgt tgtctttgat tgctaataac aatacgggaa cttttgacat gctttcgatg
     6781 gcaaagcgca catcggcgtc tcctccctca atcagttgtt ttgtatccaa atcataaatc
     6841 gccataccta attgtttggg atccacttca gctaatgctg gaatgtaatc cgccacttgt
     6901 cctgtttgat aatagtggaa attttttcca atcaattgtt ctaattcttt tacttccatt
     6961 tgaataacca gctttcattt tttatttttc tgcatgctgg aatttatgac ggcatgcgta
     7021 gatcgtaaat ggaataacta agacgataag ataagaaaca atcagaatcg tttcataaat
     7081 cccatgctgt ccagctgcga tactagcagg tggaacaaat gagatacaaa aagcaaaaag
     7141 tgaagtcaaa agacctaaac aagcaacgat tacttttaca acttttccgc ctggaacctg
     7201 ataggctctt tcaagactgt catctttaaa aaccagcttc aaatagcccg cgaaaaacat
     7261 aaagtacaac atcaggtaga ttacaacagt caatgacatc gctgcaagaa atgaaagatt
     7321 attccccccg ccgcttaatg taaagacggc tgcccagatt gttgcaacga tcccatttgc
     7381 gatcatcaaa tgagcaggaa caccgtgtgt gttttctttt gcaaaatatt caggaagtaa
     7441 tccttcttct gctgctacgt gaagtgcttt tgaaggagag gttacccaag aagcaatttc
     7501 tccgatgaca cctaaacaaa caagaatcgc aagaattttc gcaatccatg caagatttgg
     7561 atcaatgtgt cttaatagga attctaacga ttgaatcacc cctgtattaa gagacaaacc
     7621 atgttgaggt actgtagtag cgacggtcaa tccgccaaaa gtatctaaaa tgattgccag
     7681 aataaccaat acaaacattg ctaatggata atccttttta gggttttcca tttcatttgc
     7741 atgactggca gatgtttcta cgcccatata tgctaaaata aaggaaacaa aaacaaccag
     7801 cgtaggcaat ttcgaaaaat caggaatcaa tgttttcata gacagtatcg tttgtagtgg
     7861 agcgcctgaa gcgacataat ggatccctaa gccaaacaag actaatgctg gcaacaagat
     7921 accagctaca aaaccgatcc gagcaatcaa tgaagtgtat ttggttcctt ttaactgtga
     7981 gagtaaaata gcccagaaaa taatcaatag gattgccaat ttcagatatt tattgttttg
     8041 tatagcacca atacccgttg cataggataa tgcaccagtt aagaaataca gcatggtcaa
     8101 gtagccaact gttatctcaa accattggaa aaagattgcg gcaaaaccga atcgttttcc
     8161 caatgtgcga ctgacccaag tatagacacc gcctttttcc catcctttca ccgttgccat
     8221 ctctgctgcg cacaatgcca cagggataaa ccatagcaaa ccgcctaata acaagaaaaa
     8281 tactaagctg aagcctgagg tcgcgaatgt gggatattca tataatgaaa cgaccattga
     8341 gatggtcata gccaaaaagc caaagaacgt taattttttt gcaggaattt tcgttttgct
     8401 ttgttgcatg aatcttcctc ttttctattt gaatttgttt caattgagtt tgttgccaaa
     8461 gaagaaaaag tccaatcctt cttcttgttc ttcttttgta gtagtgatcg ttgtaaagtg
     8521 catcgccgat tttaaaatca ctgttgtcag cacataggca tacacggcag taatgatgac
     8581 accaatcact tgaatcccta agaagtacca gctgcttgga ttcgttagta aatggctgtt
     8641 agatgcaaag atccctatca gaatcgtacc tgtgaatccg cccatgccat gaacacccca
     8701 cacatcaagt gcatcatccc attgtttctt tttacgaaaa tcaacagcag catgacagac
     8761 gatacccgca atgatcccaa tcagcatggc actttttgct tctacataac cggcacaagg
     8821 cgtaatggct gccaatccag caacggatcc tgttagtacg tcgacaaaat caaagccatt
     8881 tccttttact tttgcaatga tcagccaagt aatcatcgca aatgcaagag cgatgaatgt
     8941 tgaaccaaat gcattgactg cttgttcatc tgctcgaagt gcaccaccgg aattaaaacc
     9001 gaaccagcca aaccaaagca gtcctgtacc gatagcagct gccataagac tgctatgctg
     9061 acttctttct cctaaaggaa tctttctttg accaagtgtg aggatacatg ctaaagagcc
     9121 gaatccagct gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc ccatctgatc
     9181 caaaaatccg ccaccccata tccagtggca aacagggaaa taaatcagca gattccaaaa
     9241 aataacaagt ataatatagc cttttaaatt caatctccct gcaaaagctc ctgtcatcaa
     9301 aggaacggta atcacgataa acatcagttg gtaaagaaag aacattaaaa atggaatcgt
     9361 tgcaccatga aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga aatctgccgg
     9421 gtttccaata acaccgccga tatcccgtcc aaaggccaat ccgaatccac caaataccca
     9481 cataatcgtt gttatcccaa cggagagaaa agattgagac atgatcgtta gtatattatt
     9541 cttccgtgct aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc aaaccattgc
     9601 tgtacataaa atcatgaatg ctgtatctcc tgtattgata ggtaatgtat tcattttttt
     9661 cgcctccttt ctacgattac gagtataaaa gaaggtgtag ttgttttttt cttctgtttt
     9721 tttgaattaa tacttgcatt tataaaagac agacaattaa taaaagcttg atgtgttgtg
     9781 ctctttttcg tttttcacac aaataaagac aaaaaataaa cggtcttctt tttatagaaa
     9841 accgtttatt ttcgtctttt tttattttta aagatatttt ttcataagga ataaagaata
     9901 cttcatataa aatatataaa aaagaaccgc agcgagtgtc gctacggttc tcaaatttgt
     9961 tcttcatttc aacataagtt tttcttcaat ttgcttataa tttacgtgca aagaatgaaa
    10021 cgactaatac taaaatgatt gcaccgatca atgatggaat gatagccatt cctgcaattg
    10081 ttggtcccca agttcctagt aaagcttgtc caattgctga acctactaaa ccagcaatga
    10141 tgtttgcgat gatccccatt gatgatcctt tattcgtgat tgctcctgca attgctccaa
    10201 tgataccgcc gacgattaat gcccaaataa atcccatttt aaattcctcc taactgactt
    10261 cttacttctt attgtacacg tggttcgtta ttttctttga ctttttgtgc acctttgcct
    10321 acagcagatt ttgctttatc tgtttggcgt gaagcaaatt gtcctgtgga ttttgctgct
    10381 tctgttactt tgtcttgtac tgtttcttga tctttttggt attcttcttg tgttttgatg
    10441 tctgcaacat tcacattcac ttcgataaca tctaaatgtg tcatattgtt tacttcagta
    10501 ctgatcaatt ctttgatttg gttgtaaata tcttcgatgt ctttaccgta ttctacgatc
    10561 acatctagat ccacagctac ttgtttctta cctacttctg tattgatacc tgctgttacg
    10621 ttatctgtgt tgactaattt ttcagccata tttgagaaaa atccgccatc taatgtcaat
    10681 agaccatcta catctttcaa tgcataaccg atgatttttt ggatgacttt gtcttcataa
    10741 gtcaattcac cattcatcat gcggttttct cctttttcat ttgccaaaac tgatccattt
    10801 gttgtattca tgtttttgtt ttccatcttt gtttcctcct atgatttaaa aaattgatca
    10861 actattcctg actgtttcaa ataaaaaccg attcctgcgc caattgctgt catgattatg
    10921 atcaacaatg tcttgaaaaa tccaatcgtt aaccataaaa cagctagcag tagtccaaga
    10981 cccccaaaga taatagggag cttgtatgct gaaaatagtt ctttcattat ttggccctcc
    11041 tattccactc gagagtgctt gtagtctctt gtatcttctt gttcttcata accgctgtat
    11101 gtgactgcca ctttgaccgt ttcttttgtt ccgagtactt tagttacttc ttcttcgata
    11161 tcttgcatca atacacctgt tttccctact aatgaagaag ttcgtttcaa gtctccgttg
    11221 acatggatat ggattcgatt tttcgttgaa cgtatccgta ctttaggtga atgaatgaac
    11281 tcttcttcgt gtaaatggga acgtaccaac ccttcgatcg cttttttgtc gattgttagt
    11341 ttgccatctt cccgtttcat aacgaaagtt cctcttgact ttggatagaa aagaacgact
    11401 agcaagatga taatagcaag aacggcaaat actacagctg cccagaaaat atattgttgc
    11461 atgtagtaat tcgttaaagt aaagaagcga aatcgttcca attgttcagg cataatatac
    11521 ggcgcattga tcaacgcaat cgctagaaat actgaaagta gcagtaaact catgataaca
    11581 ccgattattt ttaacccctt attcattttc cacctcctat aaacttattt ttaccctgta
    11641 aataactcta ccgacaatca cagtatagat agcgaacagt tagcatagca tcagtaaata
    11701 aagcgctacc aaacattgct ggatcataac ggcgttttat tgccagtctg atactgaaat
    11761 aacaattgct tacttattct ctatttactt ttgattgctg gctatactta aaggatatta
    11821 ttcaagatta aaaattgcaa agattatgct cagttattat tacaagattc tatttatt
//
LOCUS       DACDHL010000075        11733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDHL010000075 DACDHL010000000
VERSION     DACDHL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.075"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..480)
                     /locus_tag="IV228_002141"
     CDS             complement(16..480)
                     /locus_tag="IV228_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8718017.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(967..2235)
                     /locus_tag="IV228_002142"
     CDS             complement(967..2235)
                     /locus_tag="IV228_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8718018.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(2466..3203)
                     /locus_tag="IV228_002143"
     CDS             complement(2466..3203)
                     /locus_tag="IV228_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8718019.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(3270..3839)
                     /locus_tag="IV228_002144"
     CDS             complement(3270..3839)
                     /locus_tag="IV228_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8718020.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3967..4839
                     /locus_tag="IV228_002145"
     CDS             3967..4839
                     /locus_tag="IV228_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8718021.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            5181..6053
                     /locus_tag="IV228_002146"
     CDS             5181..6053
                     /locus_tag="IV228_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8718022.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            6050..8008
                     /locus_tag="IV228_002147"
     CDS             6050..8008
                     /locus_tag="IV228_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8718023.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(8259..9110)
                     /locus_tag="IV228_002148"
     CDS             complement(8259..9110)
                     /locus_tag="IV228_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8718024.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            9233..9700
                     /locus_tag="IV228_002149"
     CDS             9233..9700
                     /locus_tag="IV228_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8718025.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9963..10445)
                     /locus_tag="IV228_002150"
     CDS             complement(9963..10445)
                     /locus_tag="IV228_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8718026.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(10545..11408)
                     /locus_tag="IV228_002151"
     CDS             complement(10545..11408)
                     /locus_tag="IV228_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8718027.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         3441 a         2383 c         2140 g         3769 t
ORIGIN      
        1 ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt
       61 tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac
      121 atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc
      181 gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt
      241 tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc
      301 ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag
      361 tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac
      421 gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat
      481 aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa
      541 aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat
      601 agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa
      661 aaccaagatt atttattctt atcaaactat agaagatagc tcttctgcag tagaaaattt
      721 tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt
      781 ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt
      841 tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca
      901 tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac
      961 ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca
     1021 atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa
     1081 tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc
     1141 gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta
     1201 aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc
     1261 atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg
     1321 ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc
     1381 atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc
     1441 agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt
     1501 ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac
     1561 gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat
     1621 ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc
     1681 tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact
     1741 ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact
     1801 ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc
     1861 ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt
     1921 caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg
     1981 agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc
     2041 ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt
     2101 ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc
     2161 agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa
     2221 attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt
     2281 aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat
     2341 aaatttgctt tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct
     2401 taaattgatg gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt
     2461 atggattata cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct
     2521 ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct
     2581 ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca
     2641 gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga
     2701 cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca
     2761 ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt
     2821 tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc
     2881 atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca
     2941 ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg
     3001 acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat
     3061 tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt
     3121 tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc
     3181 actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat
     3241 actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt
     3301 ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc
     3361 gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg
     3421 atccaagcat ttttcttgat gtggattggc tttactaaca agcctcttcg ttcttttgga
     3481 tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct
     3541 aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct
     3601 atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct
     3661 actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca
     3721 gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag
     3781 agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt
     3841 ttgctccctc cttttatcct tactttataa cggacggctt atcatgtcta atacttataa
     3901 aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg
     3961 gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc
     4021 agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat
     4081 ttagaagaag aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg
     4141 gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg
     4201 gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa
     4261 actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat
     4321 atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga
     4381 ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga
     4441 ctgccagccc aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa
     4501 accgtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca
     4561 gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga
     4621 acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta
     4681 gctctagatg ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg
     4741 ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct
     4801 cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct
     4861 ttcagctttt taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt
     4921 tcttaattta tttcttcctt ttatttctga taattccagt aaattactat aattataaaa
     4981 aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt
     5041 catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg
     5101 tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa
     5161 tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg
     5221 tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa
     5281 gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc
     5341 aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa
     5401 cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg
     5461 ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca
     5521 acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg
     5581 cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata
     5641 tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg
     5701 aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc
     5761 attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac
     5821 aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac
     5881 gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat
     5941 accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa
     6001 gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata
     6061 tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt
     6121 actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt
     6181 acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc
     6241 actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga
     6301 agttccagta aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga
     6361 atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt
     6421 gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt
     6481 ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc
     6541 agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat
     6601 tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc
     6661 tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga
     6721 tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg
     6781 atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc
     6841 acgtccagaa aacaaagcaa aaacagcttc cttgatgcta gcagcagctt ttgcttcatt
     6901 ctttactggt gtgacagaac cacttgaatt ttcatttatg ttcgttgctt ggccattata
     6961 tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac
     7021 agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat
     7081 tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt
     7141 tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga
     7201 tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag
     7261 caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac
     7321 acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac
     7381 tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga
     7441 agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcag cacgacctgc
     7501 acaaacaaac accgtggtta aaacagaaac atttgccggt gaaacgatcg aacaagatat
     7561 ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc
     7621 ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc
     7681 aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga
     7741 tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc
     7801 attcacttta tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt
     7861 tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa
     7921 tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa
     7981 aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata
     8041 aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg
     8101 ttctcctgcc aactccgcgg taataagtcc agctaaacga aacatatgaa gagctatttt
     8161 ttatttgact cttcttatta cttgatgcag aacagctttt tcacaaaatt tttcttcata
     8221 ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct
     8281 tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag
     8341 gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct
     8401 tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata
     8461 atttctaact ctttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt
     8521 agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca
     8581 gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg
     8641 aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta
     8701 ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga
     8761 tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa
     8821 gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct
     8881 ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct
     8941 aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt
     9001 tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc
     9061 agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact
     9121 ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca
     9181 taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta
     9241 ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac
     9301 ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg
     9361 taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca
     9421 gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga
     9481 aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc
     9541 gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga
     9601 aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa
     9661 aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca
     9721 tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt
     9781 catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata
     9841 aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc
     9901 tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat
     9961 tatcatggtt tattgactac cggtatccag atttctccca tatatttccc gttggtaact
    10021 cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa
    10081 acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa
    10141 gcgcttacaa caagatacgt tccttttggg aatgggatga ttcgcgtagt gtttggaaca
    10201 tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact
    10261 gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata
    10321 actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc
    10381 ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt
    10441 gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat
    10501 gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc
    10561 actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag
    10621 atctcgggag cttcgactaa cccataatct gaagaaggaa gccattctgc atagattttc
    10681 ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg
    10741 tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag
    10801 ccaatcatgt gcatcaaact gcccttttct tccaagcggt ctccatcaaa tccataagaa
    10861 acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc
    10921 tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga
    10981 acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc
    11041 gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg
    11101 tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct
    11161 tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc atctagaaga
    11221 tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca
    11281 ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt
    11341 ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct
    11401 ttaagcatat ttatcttcct ccttgattgc ctttttatca tattatcaaa gaaagtcaat
    11461 tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga
    11521 aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac
    11581 ctttagaaaa ataaattttg gggaagacag attctaagta aacaaagcgg taaaaatatc
    11641 ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac
    11701 tgctgatcag aacgctgctt ggatttttct ttg
//
LOCUS       DACDHL010000076        11665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDHL010000076 DACDHL010000000
VERSION     DACDHL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.076"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(212..2287)
                     /locus_tag="IV228_002152"
     CDS             complement(212..2287)
                     /locus_tag="IV228_002152"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8718028.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2289..3206)
                     /gene="glyQ"
                     /locus_tag="IV228_002153"
     CDS             complement(2289..3206)
                     /gene="glyQ"
                     /locus_tag="IV228_002153"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8718029.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3590..4402)
                     /gene="recO"
                     /locus_tag="IV228_002154"
     CDS             complement(3590..4402)
                     /gene="recO"
                     /locus_tag="IV228_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8718030.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4545..5444)
                     /locus_tag="IV228_002155"
     CDS             complement(4545..5444)
                     /locus_tag="IV228_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8718031.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5459..5860)
                     /locus_tag="IV228_002156"
     CDS             complement(5459..5860)
                     /locus_tag="IV228_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8718032.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5838..6314)
                     /gene="ybeY"
                     /locus_tag="IV228_002157"
     CDS             complement(5838..6314)
                     /gene="ybeY"
                     /locus_tag="IV228_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8718033.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6331..8523)
                     /locus_tag="IV228_002158"
     CDS             complement(6331..8523)
                     /locus_tag="IV228_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8718034.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8547..9518)
                     /locus_tag="IV228_002159"
     CDS             complement(8547..9518)
                     /locus_tag="IV228_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8718035.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10224..11603
                     /gene="brnQ"
                     /locus_tag="IV228_002160"
     CDS             10224..11603
                     /gene="brnQ"
                     /locus_tag="IV228_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8718036.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3177 a         2440 c         2132 g         3916 t
ORIGIN      
        1 cctaatttaa aagtaaataa aacagtaaca agattattat taatcttttt catttttatt
       61 tcctcacttt tcatgagcgt tttgaaaatt ctagcaaaga ataaaataac aaaaattatt
      121 aaaatagaat taataacagc ttttatattt ataacaaaaa aacagagaca cagcacgttt
      181 cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga tccaagcttg
      241 ctaatgacaa agccatggca gttatttgac taagttgtgt tagtcggttc ttacgtactg
      301 ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt ggacgaagag
      361 cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct ttcattgctt
      421 ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg tctacttctg
      481 attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaat gcttccattg
      541 aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct gcaaatactt
      601 ttgtcagatc tttttgttct gcattcaaaa cagcttcgat gacatcatga cggatattct
      661 tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacctgt tgttgtcccg
      721 ctgacaagcc aatgccaaaa cgatccgtat ctttactgat aacttcacta atcgtttctt
      781 gcagaacaga caaaggaaag gcccattttt tcgattcgat gattcgaacg acaccatatg
      841 cttgacggcg caaagcatat ggatcgtttg agccacttgg gatcatccca actgcaaaga
      901 aagaaaagat actatctaat ttatcagcaa tcgctaaaac agaacctact gtagtctcag
      961 gtaaatctcc ttcactggat ataggcatgt aatgttcacg gatcgctgtt gctacggctg
     1021 gtttctcgcc tttaagcaga gcatattttt ctcccatgat cccttgtagt tcagggaatt
     1081 ctcctaccat gttggttact agatcaaatt tatagatttc agcagcgcgt tgcagatcca
     1141 tcaattcttc ctcagatagt cctacttgtt cgcctatgat gcgagcgatg acttgtaccc
     1201 gttgcatttt ctcatagatg ctaccgattt tttcatggaa agtaacagtt tttaattttt
     1261 caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc gcatcttcta
     1321 aacgagcagt caatactttt tgattgcctc gtaccacatt ttccaatttt acccgatcgc
     1381 cattacgagc agaaataaaa tgaggaagca acataccttg atcgttccgt acttcgaaat
     1441 aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagat agatattttt
     1501 cttcgaagtc gccagaaaag acagttggat attcgactag attgttgact tcttctagta
     1561 aatcttcatc tagatcaatg atccagtgat ttttttctgc taattcgttt gcttgtgtga
     1621 tgatcatttc ttttcttttt tgcggtgaag caataacaaa ttgttcattc aatttttctt
     1681 cgtattcttt tgcatgcgta aatgtcacat cttcgcctaa gaaacgatga cctcttgaaa
     1741 ctctgtctgt tttgacatcc aagatctcaa acgggattat ttcgtcatct aacaatgcca
     1801 cgatccagtg aatagggcga atatattcaa aatcgtattc tgcccagtgc atcgttactg
     1861 ggaaagtgag gcttttaatg acatctgcta aacctgtcaa tacttctttt gcttctttgc
     1921 cgtgagtgta tttcgttaca tagacatatt ccacgccttt taattctcta aaagtgatag
     1981 catctgtcgt cagaccttgt ccgcggacaa atccttcagc tgctttcgtc cagtttccat
     2041 ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcgctg tctgcttgac
     2101 gttctggaat gtttgttact ttgattgcta agcggcgcgg tgtagaaaac gtttcgatcg
     2161 tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct aattgtttca
     2221 tgcttggtgt gactacatga gcaggcattt cttccagccc gatttcaagt aataagtctt
     2281 ttgccatctt attcagcctc cttcacgttt tctggatttg ctgtgttttt attcaataaa
     2341 gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg agccatgttg
     2401 cggatacggg aaagataacc tgcacgctct gttacggata ctgcaccgcg agcatctaat
     2461 aaattgaatg tatggctaca tttcaaaata taatcatacg ctggatgaac aagattttca
     2521 tcgatacagc gtttagcttc tttttcaaaa cgatcaaaat tgcttagtag caggtcttgg
     2581 tcgcttactt caaaagaata tttagaatgt tcatattctg gttgtttgaa gatctcacca
     2641 tatttcacgc catcagccca ttccagatca taaacacttt caacttcttg gatataagaa
     2701 gcaagacgtt ctagtccata agtgatctca gaagtaactg gtttacaagg tagtcctccg
     2761 acttgctgga agtacgtgaa ttgtgtgatc tccatcccat caagccatac ttcccagccc
     2821 acaccggcac aacccatcga tgggttctcc cagttgtctt caacaaagcg aatatcgtgt
     2881 tctaatggat caattcctaa aagacgcaag ctttctaaat aaagctcttg gatattttca
     2941 ggtgaaggtt tcattacgac ttgaaattga tgatgctgat acaaacggtt tgggttttct
     3001 ccataacgcc cgtcagctgg tcggcgggat ggctctacat aagcagcatt ccatggttcc
     3061 ggtccgattg cccgtaagaa tgtatatggg ctcatcgtac ctgctccttt ttctgtatca
     3121 taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt taaaatcata
     3181 tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttta agcgttgctg
     3241 tcaataagaa tacaggaaag aaaacgacaa aaagtcccta tgtctgatta cagacatagg
     3301 gacgaataat ttcgcggttc caccctactt ccgaataaag attcggcagc ttcatttatc
     3361 gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc ttccactctc ccgaactcgc
     3421 taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc agttttctgg
     3481 tttgtataat agatcctctc agtgaacgat ctatttaatc tacggttaat aatagacgga
     3541 agtccctgta tttgtcaaat agttttgatt tttttgttgt atctttcatt tattcttctt
     3601 tttcaggtat ctctttttca ggcatatttt tctctggcgt ctttaatacg tgtgcccaag
     3661 acttcatttg atcaatgaac tttttgcttt ttagatgaat accgacatat tcatcataca
     3721 actggtcaat gaggtcacgg attgcctgct tcgtttcttc tttcaagtgg attccactga
     3781 tcttatcata agaaatagcc gaaaacattc gaacgaaata aacagcgcga ggatctgcat
     3841 gataacggtg ttcgtccatt ggccagtgtt tctcgcatat cacaccgcca tatttggaag
     3901 aatagtcaaa ttttcctttt gtttccccgc atacgacaca gtgcgtccaa attggcgcga
     3961 ttccgaagcg ttgcagtatt tgcacttcaa agatattcgt gatgatttct gcatcatctc
     4021 cttggtccat ccgctgcaga gcttgttgcg taaattggaa taaatgcggg tcatattgat
     4081 gatcttcgat tgctgcatct actagattta gcaaatacgt accataaccg ctcaaaaaga
     4141 tatcttcttg gatgttccga aacgcatgga cgtctttgac actgcttaaa aacgacaaac
     4201 cttcacttcg aaaattaccg atatagactg cttcggtaaa ggggagcaaa gccggagcta
     4261 atggctgatt ttgccgatgg attcctttga tataaaacat cattttgcca gaagattccg
     4321 taaagatttt gactaattta tctttttctt tgtaattacg gctagataaa atcaagcctt
     4381 tcgattctac aagctggctc atattctccc tcctaaaaaa taaatctgga aaagcagatt
     4441 ctgccgatcc agattattta tgtttcgatc ttccgctgtt tttctcaacg tacggatttt
     4501 tatccagttt atttctatac ctaacctata agaagtccag aatatcagta ctcgtctttt
     4561 cggtagccga agtcttgcag atagactttt ttgtctcgcc agtctttttg gactttgacc
     4621 caaagttcta gaaatacttt gtctcctaaa agattttcga tatcctttct tgctttcgtt
     4681 cctacttgct tcagcatttt tccgcctttg ccgataatga ttcctttttg gctatctctt
     4741 tcgacgatga tcgtggcctg gatatggatt ttatcattct cgtttcgctt catcgaatca
     4801 acaacgactg cgacagaatg aggcacttcg tctcgtgtta aaaacaagac tttctcacgg
     4861 atcaattcag aaacgatgaa gtattcagga tgatcagtca cttgatcatc tggaaaatat
     4921 tgtgggccct ctggcatttc actgactaat acatccatta agcgttcgac attgtttcct
     4981 tctgtagcag agatcggaat cacttgtgca aaaggcatct gcgtcgaata atcttcgatg
     5041 attcccagca actcgtctgg atggatctta tcgattttat tgataatcag ataaacagga
     5101 gaatgcacgt tttttagacg ttcaataata aaatcgtctc cccggccacg tttttgatca
     5161 gcactgatca taaataagac tgcatccact tctttcaacg cactgtaagc tgcttctacc
     5221 ataaaatctc ctagacgatg ctttggttta tggattcctg gtgtatcgat aaaaatcagc
     5281 tgagcttctg gtgtagtata tactccttgg attttatttc tagttgtctg tgccttgtca
     5341 ctcataatgg caattttttg acccacgatt cggttcaata atgtggattt tcctacattc
     5401 gggcggccga cgattgcgac aaaccctgat ttatgttctg gcatatacgt tctccttttt
     5461 aattaaacaa caaatgatat attttaggga caaataaaat caacccaatg atgacagcaa
     5521 aacaagcggt cactaaaact gctccagctg ccatatcttt gatttttttc ccaatcggat
     5581 gaaaatgaaa atccgtaaac atgtccacta cattttcaaa aacagtattg atgatctcta
     5641 ctatccaaac gagaaaaaca gctaaaagca gccataacca ctctatccga tcaagctgga
     5701 aaatcacgcc cataaataaa gcaaggatac cgaacgcaac atgtttcctc atattgcgtt
     5761 cttcctcaaa aacggttttg actccttgga cagcaaattc cacagacatc aggaaatgtt
     5821 tgtttttctc tacgttttta tcttttgagt ccataggcat ccaagatctc tttctgtaaa
     5881 ccaaacattt ctttctcatc ttctggtgtc atatggtcat acccgttgat atgcaaaaat
     5941 ccatgtaccg ctaaaaagcc aagttcccga tcatatgaat ggccgtaagc ttctgcttgt
     6001 tcagcagcac gttcaataga gatcatgatg tctcctagta cacgaggtaa tggattctct
     6061 ccatcttcaa aaatcactgg gatttcgtct tcgccttctt cttccagagc aaaactgatg
     6121 acgtctgtcg gtgcatcttt tccacggtaa tcacgattga ttacttgaat tgccgcattg
     6181 tccatgaaag taacagacat ttctgtttcc tctggcagct ttaaataaga tgctgcaaat
     6241 tgcagcaagt catcgatttc tttcattttc tcttctgaga cttggtttgt ttcatccata
     6301 aaggtaatat ccattataca tcgcttcttt ctattcttct tgtacttcct ttatcttctc
     6361 aacttctgtt ttcattttag gatatttgat ccgagaatgg aaggtgctac ttaaaccgct
     6421 gattaatgat ttttccactt ttcggatttc tttcaaggtc agtccactat cgtctaattg
     6481 accatcggaa atacgctctt cgataagatt atggacaaaa ctttcgattt tttcgctcgt
     6541 cggatgatcc attgcccgta ctgcagcctc acaactgtca gcaatattga cgatccctgc
     6601 ttcacgactt tgcggttttg gtccaggata acggaattgc tcttctgtta cttccggatt
     6661 gcgttctttc gctttgatgt agaaataacg cattagcgtg gttccatgat gctgtttgca
     6721 aatatcgatc accatttgcg gcatattata atcttccaat attttggctc catctgtcac
     6781 atgtccaaag ataatatgtt tactgtcttc tggcaataag aaattgtgcg ggttttccgc
     6841 tcctgaaggc aagttttcaa cgaaaaagct agcatgtttg atcttgccga tatcatgata
     6901 atagcaagct actcgagtta gcaaagaacg tccgccgatt tctgctacag cgttagcact
     6961 tagattagct accatcatac tatgatgata agtacctggc gcttcttcca gcaactgttt
     7021 caataaagga tgatttggat tgctgagttc atttaataca attacactgt catcattgac
     7081 catcaattca atatatgggt gtagaccgat cgtcagcaag aatgagaaga gtgcaccagt
     7141 gaacccgcag acgagcatta accaagtcgt tgcatcacca aagctcattc cttgatagac
     7201 gatcaaaatg acatccatca tcacaggaaa aataatgacc cacatggcag cagataatcc
     7261 ttgttctgag atccgtttgc gtctgacaat cgttgccatg agcccagaga aaacataaga
     7321 catcaagata atcgttagaa agttcgtgcc aatcgaatca taaaaaatga aaagtgcgga
     7381 tactgcttgg aaaataccag ccatgatccc tgctcttcga ttcaagaaaa agcttaaaac
     7441 aagtggtgca aaagcagcgg gataaaacag cgggatataa gtagcttgtt ccgtttggaa
     7501 taattggaaa aatttcatca gcactatgct gactgacata gctgtcacat agaaaagcac
     7561 gtattttgtt ctttctcctg cattttggaa ttgaagtgaa ttataaatca gtacgccgat
     7621 ttgaagcaaa acagctaaaa ccatggcaat tagcggaaaa atcgaagttg tttgattagt
     7681 cagtcctagt aattccaatt ttttcatagc tgaagcatcg atttgattac cttcacgaac
     7741 aatgatttct ccttggaaga tcatcactgg ttgtacagct tctcgtgcgg attgtttcaa
     7801 ttcttctgtc tttttctcat tcaatacgtc attcactacg ataccttgat ccactaagta
     7861 gcgaatcatt tgttgttgtg ctggtgtgac attcaaatat tggatttgct cttctgcttc
     7921 ttgtttaaaa gcttccaatt cactttcacg aacttgtttg ctcatctgtt cgtcaatgag
     7981 cttcagactc tcatccctca cagtatgaat ctcagtttca gtcatttcaa atatcgtcga
     8041 ataaaaattg ttcggtagtt tttgataaaa cacaacattt tctgcattca cattttcaaa
     8101 atttttcttc agcgcggcga tccgctcttc tgtagtaggc tttgggatat tttcatcttc
     8161 ctttgcctta tcaatctttt cgtcataaga tttgttgatg gcatcattcg tcttatcgat
     8221 caattcaaat agttgcttga tccgattgtt ctggtcatct gcaagatctt tttggtaagt
     8281 gtattctgga gtgactgctt ctgctgccag ttttcttttt tgttcggttt cttccgtgtt
     8341 ctcgatcgtt ttgttcgcac ggatactttc ttctgctact tggccttctc tgaaatcatt
     8401 ggccttttgt ttgacactac taaatgtaac gccgcagata atgataaaaa acaagaacag
     8461 caggaaaggc aatgctgcct ttccaagttt ttcttgtagt ttctgcagtg ttttattcaa
     8521 catgtgctta tcctcctaag caaagcttat tcttgatgga gatctgcctt ttcataagct
     8581 ttgatgattt cggcaactac agggtgccga acaacgtcac ttgcttcaaa attaacaaaa
     8641 tcgattttct tgatttgctt taatgttcgt tctgcatgaa caagaccact tgtcgtccct
     8701 cgaggcaagt ctatctgact tgtatcccca ttgacaatca tttttgattg ataaccgaga
     8761 cgggtcaaga acattttcat ctgagcgact gtcgtgtttt gtgcttcgtc caagataacg
     8821 aatgcatcat ccaatgttcg cccccgcatg taagctaaag gtgcgatttc gattacgcct
     8881 cgttccatca atcggttcgt atgatccaat ccaaaaattt ggtataaagc atcataaaca
     8941 ggccgcagat aaggatccac tttttctttt agatccccgg gaagaaagcc tagattttca
     9001 cctgcttcca cagcaggccg cgtcaaaatg attttttgca cttcaccttt tttcaaggca
     9061 gcgattgcta atactactgc aagaaaagtt tttcctgtcc cggctggtcc tacgccaaat
     9121 acgatatcat gtttggctac cgattcgaca tatttctttt ggccgctgtt ttttacccgt
     9181 ataggttttc cattacggtc tttgacgatc tcttcctcat acatctcaac aaaataatct
     9241 aatgtacctt tttgccccat tttcaatgcc gtcacaacat cgggcgtact tacgtggatt
     9301 cctcgtttga tcaattcctg taatgtccga atgactgagg cagccaagga aatctgggat
     9361 tgctcaccaa tgatctgaat gatttctcca cgtgtatgga tggttgtttc cgttgtttcc
     9421 tcaatcagtt ttatatgctt gtcgtgtgca cctaaaagca tgctgacatc atcagcagcc
     9481 gtcaatctaa tctctaaaga actagactct tccgtcaaaa atagacatcc cctttttttc
     9541 gattaattct ttcctatttt accatactca gaagaaacta tatagcaaat aagaaaaggt
     9601 tctaaaagag ttgcttaacc ataagtacca agaaacgtta aaaaactagc cgcttccatt
     9661 ctctaaaggt ctcgtttgaa taaaaaaagc tgatcatgta agctatgcat aagatagagg
     9721 acaagtattc cgtctaactt agcaagtatt ctctgggtat ttttcttgtt tcaaaaaaag
     9781 aaaacaaaat atttgaaata ttcttgcatt tcaaaaaaaa caggtgtatt ataacgtcaa
     9841 caaataaata tacagcaaag cgtcgaggaa aacagtaggc tattcagtga tttattccca
     9901 gtgagtcatg taagtgagaa atgacaataa atcgaataga cgaacatcat ttccgagtca
     9961 atgtcgaaaa cgtagatcat tgtggctgcc tccattatca ggctagcgga taactttatc
    10021 gaaaagcgtc cgtgaatgaa gaagcttttt taagcgaaaa aaggtggtac cgttctagaa
    10081 tctagagccc ttttcccaag tggaaaaggg ctttttttat attttaaggt ggtggttttc
    10141 atggaatctg atattagtgg tatttctagt ttctgcaacg tttattttgt tactcaaaat
    10201 gataattatt ggaggaaaaa tttatggaag aaacaaaaac ttattcatgg aagcaattat
    10261 tgatggtcag ttcacttatc ttcgggatgt tcttcgggtc aggtaatctg atctttcctg
    10321 tacacttagg tcaaatggct ggatcaaact ggatcagcgc tggtgtagga tttgccatct
    10381 caggcgctct tttcccatta ttagctatct tagctgtagt agtgacaaaa agtgatggtc
    10441 tgtatgactt agctaaacct gtcggaaaat ggtatgctgc cttgttcttg atccttgttc
    10501 atttaacgat cggaccattc tttggtacac cgcggacagc agcaactgct tttgaaatgg
    10561 ctgctaaacc ctttttgcct gaaaaatatg attcattagg tatgttttta ttctctgctc
    10621 ttttcttttt acttgcttat ttccttactg tacatccaac acgtttggtc aaatatgtcg
    10681 gaaagtattt aaactccttg tttcttgcat tattagctat catttttgtc gtttctttat
    10741 tccaacccat ggggaatcta aaccaatctg ctgcagattc ttatcagacg aacgctttga
    10801 caaatggcgt tttagaagga tacaacacgg ttgatgcagt agctttactt gctttaagtg
    10861 tcacttttgt ccatgcagtt cgtgatcttg gttttaaaga taaaaaagtc acagaattaa
    10921 cagcaaaagc tggtagcctg agcgttgcgt tagaagtcat gatttatttt ggtctggtcc
    10981 ttctcggtgc gatgagtttg aataagatgt ctttatcaga aaatggcgga actgcacttt
    11041 ctcagatcac taatttttat ttaggtaact tcggtgctag cttcttaggg gtaatggtca
    11101 cattaggtgt cttcactact gctatgggac tcgttgtttc ttttgcacaa gatttccata
    11161 aactgttccc taaagtcagt tatatgacat ggctgcggtt aacgactttt gtctcttttg
    11221 tcgttgcgaa tgccggattg gataatatca tccaatggtc tttaccagta ttgatgcttt
    11281 tgtatccatt gtcacttgcg ttaatcttac tttccttaac agcaaaattc ttccaaaaga
    11341 gtccatttgt ctatcaagta acgatgttct tttctgccgt tccagccgta ttggatatgt
    11401 tggcaaattc gcctgcatta gtgagtcagc aaactgtcgt agcaagctta ctaggatttt
    11461 atcatcatta tgttcctttt gctgcattag gtcttggctg ggtcgtccca acattgcttg
    11521 gttacgctgg cagcttgctc ttttattatg tatatcgcgt atcaggatat aaacaagaag
    11581 caagtgagtt accagaagaa taaccaaatc aaacacaatt taacataaga gatattaaac
    11641 aggaaaaaaa cagctagaag aaaga
//
LOCUS       DACDHL010000077        11409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDHL010000077 DACDHL010000000
VERSION     DACDHL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.077"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(69..1490)
                     /locus_tag="IV228_002161"
     CDS             complement(69..1490)
                     /locus_tag="IV228_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8718037.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="IV228_002162"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="IV228_002162"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8718038.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /locus_tag="IV228_002163"
     CDS             complement(3901..5157)
                     /locus_tag="IV228_002163"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8718039.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            5737..7038
                     /locus_tag="IV228_002164"
     CDS             5737..7038
                     /locus_tag="IV228_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8718040.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="IV228_002165"
     CDS             complement(7107..7706)
                     /locus_tag="IV228_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8718041.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7961..8842
                     /locus_tag="IV228_002166"
     CDS             7961..8842
                     /locus_tag="IV228_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718042.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8855..10465
                     /locus_tag="IV228_002167"
     CDS             8855..10465
                     /locus_tag="IV228_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8718043.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10542..11201)
                     /locus_tag="IV228_002168"
     CDS             complement(10542..11201)
                     /locus_tag="IV228_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8718044.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT         3324 a         2281 c         2075 g         3729 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     7801 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
     7861 aaaataataa aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc
     7921 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
     7981 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
     8041 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
     8101 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
     8161 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
     8221 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
     8281 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
     8341 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
     8401 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
     8461 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
     8521 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
     8581 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
     8641 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
     8701 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
     8761 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
     8821 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
     8881 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     8941 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     9001 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     9061 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     9121 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     9181 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     9241 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     9301 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     9361 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     9421 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     9481 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     9541 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     9601 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     9661 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     9721 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     9781 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     9841 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     9901 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     9961 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
    10021 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
    10081 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
    10141 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
    10201 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
    10261 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
    10321 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
    10381 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
    10441 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
    10501 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
    10561 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
    10621 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
    10681 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
    10741 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
    10801 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
    10861 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
    10921 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
    10981 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
    11041 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
    11101 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
    11161 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
    11221 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
    11281 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
    11341 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
    11401 ttacttttt
//
LOCUS       DACDHL010000078        11249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDHL010000078 DACDHL010000000
VERSION     DACDHL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.078"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            174..443
                     /gene="rpsN"
                     /locus_tag="IV228_002169"
     CDS             174..443
                     /gene="rpsN"
                     /locus_tag="IV228_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8718045.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            458..637
                     /gene="rpmF"
                     /locus_tag="IV228_002170"
     CDS             458..637
                     /gene="rpmF"
                     /locus_tag="IV228_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8718046.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            669..1046
                     /locus_tag="IV228_002171"
     CDS             669..1046
                     /locus_tag="IV228_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718047.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1067..1216
                     /gene="rpmG"
                     /locus_tag="IV228_002172"
     CDS             1067..1216
                     /gene="rpmG"
                     /locus_tag="IV228_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8718048.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1240..2223
                     /locus_tag="IV228_002173"
     CDS             1240..2223
                     /locus_tag="IV228_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8718049.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2253..2375
                     /locus_tag="IV228_002174"
     CDS             2253..2375
                     /locus_tag="IV228_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8718050.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2393..3916
                     /locus_tag="IV228_002175"
     CDS             2393..3916
                     /locus_tag="IV228_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8718051.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            3940..4179
                     /locus_tag="IV228_002176"
     CDS             3940..4179
                     /locus_tag="IV228_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718052.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4372..5007)
                     /locus_tag="IV228_002177"
     CDS             complement(4372..5007)
                     /locus_tag="IV228_002177"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8718053.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5028..5246)
                     /locus_tag="IV228_002178"
     CDS             complement(5028..5246)
                     /locus_tag="IV228_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718054.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5555..6382
                     /locus_tag="IV228_002179"
     CDS             5555..6382
                     /locus_tag="IV228_002179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP8718055.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6571..6978
                     /locus_tag="IV228_002180"
     CDS             6571..6978
                     /locus_tag="IV228_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8718056.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(7219..8559)
                     /locus_tag="IV228_002181"
     CDS             complement(7219..8559)
                     /locus_tag="IV228_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8718057.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(8835..8990)
                     /locus_tag="IV228_002182"
     CDS             complement(8835..8990)
                     /locus_tag="IV228_002182"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8718058.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9287..9619)
                     /locus_tag="IV228_002183"
     CDS             complement(9287..9619)
                     /locus_tag="IV228_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8718059.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10133..10324)
                     /locus_tag="IV228_002184"
     CDS             complement(10133..10324)
                     /locus_tag="IV228_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718060.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10422..10808)
                     /locus_tag="IV228_002185"
     CDS             complement(10422..10808)
                     /locus_tag="IV228_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8718061.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3728 a         1787 c         2161 g         3573 t
ORIGIN      
        1 attatctcta tgtaccgata aattcgtttt caaatagtta tctattcata tgagttttat
       61 ttcatgttat ttattgttga caagagcaac tcttgttggt aaagtatcag tgtcaaaacg
      121 taacgattac gatttgagat gtctaaatca aaataaaagg agtataaagg tatatggcaa
      181 aaaagtcgaa aattgctaaa gcaaagaaac aaatggcgat gattgaaaag tacgcagata
      241 aacgtcagga attaaaagcc gcaggagatc gcaccgctct cgctaaattg ccaagagatt
      301 caaatccgaa tcgtctgagg cttcgtgatc agacggatgg tcgtccaaga gggtatatgc
      361 gaaagttcgg tatgtcccgt attaagttta gagaacttgc ccatcaaggg ttgattcctg
      421 gagtaaaaaa agcaagttgg taaaaggagg aaatatcatg gcagtaccag caagaaaaac
      481 atctaaggcg aagaaacggt taagaagaac tcatcaaact gtagtaaaac cagaaatttc
      541 atttgacgaa gcgaatggtg attatagacg tagccatcat gtatctttaa aagggtacta
      601 taatggaaag aaagtaatta aaggagcttc aaaatagttt aatatataac ttttaggagg
      661 gaatcaaaat ggaaattatt tacccacctt tagttgaaga aggattaaag tattatcttg
      721 aaacgaccca gcaatcgcta gataaatgca ctttttatcg ttcgatggta gagagaggaa
      781 ttattactga aacgggttta ccgacccaac aggcaattga aaatggatta gtcaaagatt
      841 actatgagga ccaaggctta tcatttgatg attttttaag aatctatcca atttttgaag
      901 agtatgacga ggagttattt caatgtattg atggttattg ggagatacct gttgatatga
      961 aagaaaattt agtttcacaa ttggaatctg gcgagttaac ttttgaagat gcacaacaaa
     1021 ttcaagcata tcttgaagat cgataagtat tgaaggagga agatttatgc gtcaaaatat
     1081 tattttggaa tgtgtcgaaa ctggagaacg tctgtatctc accagcaaaa ataaacggaa
     1141 taaccctgaa cggttagaat taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt
     1201 caaagaagtg aaatagctaa gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc
     1261 gtttctgggt ttttaggggc tggaaaaaca acgctaatca atcaagcctt gcaagtctca
     1321 ccctacccca gggaagaaat tattataatt gaaaatgagt ttggtgaagt tgggattgat
     1381 cacaaattat tagttcatag cgaggaacgg gtgttacaac taaataatgg atgtatgtgt
     1441 tgcagtcttc gcagtgattt attagcaata ttgatttctt tactggaaac aatagaagaa
     1501 catcaacaac caatttcaca aatcatcatt gaaactacag gtatagcaga tccccaacca
     1561 atcattcaaa cacttttaac agcgcctcaa ctaagaggaa gagtatatat cgatagttta
     1621 ttgactgtta ttgatagtga cagttttgaa cttattgaaa gagaaccaca agcccttaag
     1681 caacttgtca tagcagatcg catttttgtg tctagaaaac aaaacttgaa tttatctgca
     1741 ctaaaaaaat tacagagaga aatcaaaagt attaaccctt tatccgatat tcgaatgttc
     1801 acacatagtg aaacgattca aaaaaaggat ttttataatt tggaaaaatt caatcatccc
     1861 ttagtttttg aaagtgattc atcagaaaat cactataatg gcaaacacta tcatcaacat
     1921 catcaagaac atgagtttaa atcaattctc ttaacttcag atcaagacat aaaaaaagat
     1981 tttttattcc aattaattga ccggctgatt ttaaccaacc aagggagcct gtatcgtttt
     2041 aaaggattga ttgctttgca agatcaagat tttatagttg ccatacaagg ggtaaacgaa
     2101 caggttaatt ttcaatacac cactcgacaa aaagaccaaa ccactattat tctaattgga
     2161 aaaatgttgg atgaagaaag gatcaaacag agttttgata aattactggc ggaaagcaaa
     2221 taatgataag tattgattag gggtaatttc taatggagaa aacggatata tctagtgcat
     2281 atcgacgact taaaagcccg aatatcaaaa ctagaaaaag ggcattaaaa attatcaaag
     2341 aacataaaca aaataaaatg aagaaattag cataattaag gaaggaaaaa gaatgaagaa
     2401 aatcagtgta ggacttattg ggatagcagc tctaggatta ttgggagctt gctccagcac
     2461 taatgatgca aaagtatcga atgataaaga tggaaaatta gaaattgtaa caacattcta
     2521 tccaatgtat gatttcacca aaaacatcgt cggcgatgaa gcgaacgtgg atttaatggt
     2581 tccagcggga tcagaacctc atgactatga gccctcagct aaggatatgg caaaagctca
     2641 tgatgctgat gtttttgtat atcataacga aaacatggaa tcatgggtac caaaagctaa
     2701 agaaagttgg aagaaagctg ggcctaatgt agtagaagga acaaaagaca tgattttatt
     2761 gccaggtagt gaagaagaag atcatgatca cggagaagaa gatcaccatc atgaactaga
     2821 tcctcatacg tgggtttcac ccaaaatggc aattaaggaa gtttcaaata tcaaggatca
     2881 gttagttaaa ttgtatccga aaaaagcaaa agtatttgag acaaatgctg aaaagtattt
     2941 gactaaactg aaacggttgg atgctgacta taccactagt cttaaagaag cgaaacaaaa
     3001 aagttttgtg acacaacatg cagcctttgg atatttagct ttggattatg gattgataca
     3061 agttcctatt gctggactaa gtcctgaaga agagccatct tctggtcgat tagctgaatt
     3121 aaaagagtat gttaaaaaaa ataagatcaa ttatatttac tttgaaaaga atgctaatga
     3181 taaaatcgca aaaactttgg ccaatgaagc tgggataaaa ttggaagtat tgaatcctct
     3241 tgaaagtctt acaaaagaac aaatggataa tggtgaagat tatgtttcag taatggaaga
     3301 caatctaaaa gcgttagaga aaacaactat ggttgctggt gaagaggtag ttccagaaaa
     3361 agaagcgaag gatgaaaaga cagttgctaa tgggtacttt aaagatgccg atgtgaagga
     3421 tcgcgaattg agtgattata caggcgaatg gcaatctgtt tatccgttgt taaaagatgg
     3481 aattttggat gaagttttcg attacaaagc aaaactcaat aaagacatga cagctgccga
     3541 gtacaaagat tattatacta ctggctataa gacagatatt gatactatca acattaaaga
     3601 caacaccatt gattttgtag tcaacggtga acatcatcaa tacacataca aatatgttgg
     3661 atacaaaatt ttgaattatg aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga
     3721 tgatgcaggt gctggtcgtt ttaaatatat tcaatttagc gatcatggaa ttgcaccaag
     3781 taaggcagag catttccata ttttctttgg tggagaaagt caagaaaaac tttataatga
     3841 aatgcataat tggcctactt tctatccagc ttcattatct gagcatgaga ttgctcaaga
     3901 aatgatggcc cattaaaatc caatctttgg aggaaataca tggtaagaaa agtattattg
     3961 tctatggatt ccaagaatat tgatgcttat cggcgttatc aatttgcaaa gcaaatgaga
     4021 ttaaatggga gtacattagc tgatattgct gcaactttac atgttagtat tgacacagtt
     4081 catagagtat taaaacggga tcctgattgt tgttttatca aggtcaatac tggaagaagt
     4141 agacgtttag caaaacggca taatagcttg gccaattaat tgataatacc agagtagatc
     4201 atttttccct tatttggatt tgatctgctt tttattattt cttgaattgt caaatcaagc
     4261 gcaacacttt atcaaagaag acttcataag gtgttctcca atctaaacac tttctaggtc
     4321 tgttattcag tttttcaatc atctgattta tcgttgagtt atctaccgta gctatctctt
     4381 tcgattttgg caagtattct cgaatgagtc tattcgtatt ttaatttgtt cattgttgcc
     4441 aagggcatga ggatctggaa aataaaaggg catattattc aatacttctg taactgcgaa
     4501 atgattggag aattcttttc ctctatctgg agtgatcgtt ttgaccgcct catttggaat
     4561 actgcttaaa agttcaatca ttttctcgct cacataagcg gctttctttc tggggacctt
     4621 ctttcctgct aaaaggaagc atgattttct gtctgtcaaa gtcactaaac aagtaccact
     4681 agttttgcca gctacaatat cggctttcca atgaccaaaa tcacttctat catctgcgga
     4741 ttttggatgg ccatggatcg aatgagtaag gcaaatcttc ccacgagact cgacatgatt
     4801 ttttgtatgt cttgtttttc ctcgatggtg caatgaacgg atcaggcctt tattgccgtg
     4861 tgagaggggt tctgtatcga aaagatcatt gcaaattgca cggtagatcg tgataaaact
     4921 aattgaaaag tccatatgtt catgcttcaa acgattcgag attttctctg gcgaccactg
     4981 aaaatcgaca aataaatgac gaacaattga ccaaactcac ggatcattta gaatgtgata
     5041 acgtccgcat ttttttcttc gataagcata atttttttcc gctttggaag gtgattagtt
     5101 tttttcttgt ttcttctcag ttcatgtaaa atagtaaacg ggctatgctg aatgtcgttg
     5161 ccaattatac taatagaaaa tccttcttcg tgaagcagaa agattctttc tcgtttgagt
     5221 atagtaagat gacaatactg gctcataatt attttccttc taggagtgtt ctcgacaaac
     5281 ttatactagt tggatatcga ttatgactca tttttatgtg ttgcacttaa agtgtaaatt
     5341 caaggtatag aaagaggttc cctatgaaaa aatacctatt acaatcttaa ctggctatct
     5401 tggttcagga aaaacgatac ttctaaatca tattctgtac ggtgatcacg gaaaggaaat
     5461 tgctgtcatt gtaaatgaca taggggaagt aaatgttgat ggggtattag tcgacaagaa
     5521 aggttttaaa agaactggag aaaaacttgt ttcgatgccg aatggatgca tttgttgtac
     5581 attacgcgaa gatttgattg ttgaactaga tcaattagtg caattttctg acttgaatca
     5641 aattgttatt gaagcttcag gaatcagcaa accaattcca attattcaag caattatatt
     5701 agctaaaaac cctttagaca ttgatttgac ccaacgatta gagattggtg ccattattat
     5761 agtagtggat gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga ttcaggaaag
     5821 aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt taattgatca
     5881 attcgagtgt tgtaacatat tacttttaaa taatagtgac ttagtgtcaa ccaagacgat
     5941 ttacaaaatt aacggtctaa ttgctaagtt acagtccaat gccaaaataa ttaggacaat
     6001 taaaggtgaa gtagacttat cgaaaattct aaatacaaat ctttttaata tagatgagac
     6061 ttcctattca atgggctggt tcaaagagtt agagcttggc tatcaaaatc atattcctga
     6121 aacagaagaa tatggtatct caagttttat ctatcgaaat cggttactcc ttgacgcaca
     6181 aaaatttgcc catcttacgg ataaaaattt tcctggcaat attacaagag caaaagggtt
     6241 tctttggttt gcccaacata gtgatttttc gacgatcttt gaaacatcgg gtagccataa
     6301 aagtattgaa tcattaaatt attgggtagc tagtttacct aaaattgagt gtcaaatgat
     6361 tatacaaaaa aacccgaatt gaaggaagcc tggcatccgt tgtttggtga acgcattaat
     6421 aaactagttt ttattggtat gaagatggat caacaatgta ttaaggaaca acttgagggt
     6481 tgtttaactg attctcttaa tgcagataat ttccgaaaaa tttcaccatt tccatggaaa
     6541 aattctaata tgtttagcca gatatcgtta atgaaatttt taagaaaagc tggtgtcaag
     6601 ataggcgatg tcataattat tggcattttg attattccct cttttattcc cttatttgta
     6661 ttctctatag aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc catcgttcaa
     6721 attgatggca aggaaatgga tcgcttcgat ttggataaga tcaatcataa actcgtgaat
     6781 tatcatcctg cgaagggaca atataatatt gttgagatta aagatggaag aattcgagtt
     6841 aaagaagata atagtcctga tcaaatcgcg gttagaactg gatggatctc aaaacccggt
     6901 caaacgagta tttgtttacc ccataagtta gttattagta ttgagaaaaa agaaagcaaa
     6961 gattactaca tttactaatc tggaaataga tgaggaggtt tttaataaga aaaagatatt
     7021 attattaata atagcagggg ctttattaag tgtgttaagc gcttgtggac aagaaaaaga
     7081 agcggtgaca acaggcacaa gtatctttaa cgggacctgc aatggtttag ttaacagtcg
     7141 gttagattac ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata
     7201 atcgagatac tcacacggct agacagctat cttgtctagc cgtgttgttg ttctggcttt
     7261 agtcagttgg gtagtgttct tagttttctt gaaatatttt cctcgatata aaaagattcg
     7321 ttctcggtag ttttgaaagt ttctaaagcc ataggcaatt cgttttagta ctttgatatg
     7381 gttgttcaaa cattctatag ggccgttgga atagggaact cgcaacgctc gtttgatttc
     7441 tgttcgatat ttcttgaagg tcgtgaacac tggttgatag tgctctggca actccttgta
     7501 gtcttctttt aggagcgcga cgaattcagg aacatcttgt cttctgatag cggaaagaaa
     7561 aacctggtag acttcgtagc ctcgctttaa ggagtcatca taatataata gccgatcgac
     7621 tatctccgct tctgtcagat gatctcgaaa tgacggacgc cagtagcgat gctgaccatt
     7681 caattcccac gcattctttt gaagtagtct ccagtatttc tttaagtgct tcgaccgacg
     7741 atcccctttt ttaaaggtct tcattacttg aattcggtgg ttggtaaagg cccgaccgat
     7801 gtgttggcta atatgaaagc gatcggcgac gattcgggca ttcggaaaac actctttcac
     7861 caatgtgcga tagggtgtat agaaatctga aaccactaga cgtacttgtt cacggacttt
     7921 tctagggtag cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt ccacaatatc
     7981 aatcagttga tgggtcttgc cgtccatcat gacaaagctc atggctcccg agacgttttt
     8041 caccgatttg aattcatcga aacacaccac ttctggtaac ggctggttca aagaaacggt
     8101 tttaggccga aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt gcttcatacg
     8161 tgcaatcaaa gacatagaaa taggctcact taatagctca agaatggctt gtttgactct
     8221 gcgggcaatg gagcagcgac gatcgctaac tgaatcttct gctaaaaaag tctttccaca
     8281 gtgtttacat ttgaagcgct gtttgttaat ccgcacaatg gtcttgtagt tactgacatc
     8341 attcaataag atggatactt ttttccagcc ccatttaatg atcgtttgct tattttgata
     8401 gtggcaatgg aagcattcct ctggttgata ggtcaatgtg ccatcaaaaa caaaacatgt
     8461 ttcgtcatta atgcgctctt tgcgaaaggc atcttcatga aaggtaagat ttaggtctag
     8521 aatatctagt aactctttgg taaactctga ataggacatc ttgaaacact cctttgaatg
     8581 gttttcgtcg actttattct aaaggatttc aaggtgtctg tttgcgttta ttagaaaaaa
     8641 gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagatc ccttattttc
     8701 ttcgcttcaa attgtcaagg agtcatcata aattttgtat ggattctttt gtttgggtag
     8761 tagtcttcta aataatcttg gattaacacc ttcatctcta ttttactttt atatttgttg
     8821 gagtcgatta attctcattt gattgatcga tgaaacgctt caattactgc attgtcaaca
     8881 ggagtcccag tttggctcat tgaatgtgtg atgtggtggt cggtcaacag ctcttgataa
     8941 ataaaagacc gatgctggct acctctatcg gaatgaatta ccgtaggcaa ttctccttaa
     9001 taaatttgta atggacggac gacaatctct tttttcatgt gtgaatccac catatagtaa
     9061 cgtagaatac gagttgctaa gtcaatatag gtggacaaat agaggcgacc gtcattacat
     9121 ggaatgtaag taatatccgt cacccaaaca gcatcaattt ggttctgttc aaattatcga
     9181 ttcaacattt tttttaccat ttctttgatt gttttttctc taccatgaga agatttgcgt
     9241 cgatgaaatc ctcttgtgca taaattcatt tcacgcataa gaacagctac aacacgttta
     9301 ttgatttcga ttccttcatc gtaaagtttt tgagtgatcc ctggagatcc gtatcgatct
     9361 ttgtgttcgt agaacactcg tcttcctctt ttagtcgttt attttcctgt tcaagaatgt
     9421 agaaatctgc tttatcaggt aatctttttc cttgtccagc aaaagcattt ttcccgtatc
     9481 gacgatattc tgaaacccat cgggaaattg ttggacgaga gatattgtat tcttcagcaa
     9541 ttttcttggt agttactttt ttctcaagga tatcctgtga aatctttagt ttaaatttac
     9601 gatcatatgt ttttttcaat agagttcatt ccttccggta acgtaactga tagtcaagaa
     9661 aagtgtatca tagcccaact tagtatcagg gtttgtcaac taaactgtgg aagttgatag
     9721 ataagagttt ttaacaacca aaattctctt gactattttt gaaatcagac aaattttgat
     9781 tggacacata gttgaataaa tctaccgtcg ttacggaagg taaatcgcat gcttttcaat
     9841 tctagaggag aagtcctcgt tgaagtacca tggcccagtt ctggattcga cctcctgccc
     9901 acttcgtacg taaataaagt aattttagga gtgagactgt gataaaagtt atttttaagc
     9961 taaaaacgaa ttaaaaaacg ttaataatta aaatcattcg ttttagattc tatgaatcat
    10021 tatagaacgc tgtaaaatcg atactttata attataaaga agatggcttc aaatttattg
    10081 ttcgaaattt gaaactatct ttttacttat gtcacagact cccttagttg agttattctt
    10141 taatttcgaa tttcgagata gctagatata ttgcaagcat acattcttga taacaatctt
    10201 gatcatattt tccagttctc caggattgct tatggaccaa agcgctaaat cttttgagaa
    10261 tggccacaac tgcttcttca tttccattct ttgctaaaat gaaatcttct tttaaagtat
    10321 tcataaattt accttcttcc catgaactta taatctattt attttttggg gaaggaggag
    10381 gacgagcaaa gaagcataca aaattagtta ttatgcagat attaaaccag cattttttga
    10441 atttttttgt aaagtcgatg ttttaaattt gtcgcaccct gtcttgtaat acccagaacc
    10501 tttccaattt ctttatcagt tttattaaag ataaattttt cgataagaaa aatttgttca
    10561 ggttctttca acaaaggaag gatttttgct aattttatat cttcaacaag gagagtcaca
    10621 tttaaaacat taatcggttg tttaaatttt atagtatctt tagtaaacaa atatggtggg
    10681 agttgatcaa gttgttcatg tttttgaatt tcttcttgtt ttttataata agtattagcg
    10741 gtgttcaaaa tgaccttttc aaagaatcga atcaactggg catttacgcg ttcgtcgtta
    10801 tctgacatat tgcacacctc ctccattaag atttaaaggg aaaagaggca aaaaacacga
    10861 aaagaagcta atgcaattat gctgcgctgc gaataacttc tataagctca catgcattga
    10921 taggactgct gatgtttatt ttcaaaaatg tttttttagt tgataatgga tagttatctt
    10981 tatacttaga aaagaaaatt catatatctt cttaaatatc aaacatgtcc ttatggctaa
    11041 gaaaatgtct tgagcttttt acgtataata aatcttttct aaaacctcct tttggataat
    11101 attcttattc gtgtatagat tgtatttttt atttcttccc ccttcataat taacaactag
    11161 aaaagagttc gaatagacac gtatttgaac gaaaattttt attggattcc caaattggag
    11221 tatttagaaa cgaaaaattc ctattatct
//
LOCUS       DACDHL010000079        11202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDHL010000079 DACDHL010000000
VERSION     DACDHL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.079"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            219..1454
                     /locus_tag="IV228_002186"
     CDS             219..1454
                     /locus_tag="IV228_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8718062.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2453..2713)
                     /locus_tag="IV228_002187"
     CDS             complement(2453..2713)
                     /locus_tag="IV228_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8718063.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2740..4041)
                     /locus_tag="IV228_002188"
     CDS             complement(2740..4041)
                     /locus_tag="IV228_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8718064.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4333..4791)
                     /locus_tag="IV228_002189"
     CDS             complement(4333..4791)
                     /locus_tag="IV228_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8718065.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4804..6129)
                     /locus_tag="IV228_002190"
     CDS             complement(4804..6129)
                     /locus_tag="IV228_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8718066.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6146..7558)
                     /locus_tag="IV228_002191"
     CDS             complement(6146..7558)
                     /locus_tag="IV228_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8718067.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7575..8339)
                     /locus_tag="IV228_002192"
     CDS             complement(7575..8339)
                     /locus_tag="IV228_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8718068.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8443..9318)
                     /locus_tag="IV228_002193"
     CDS             complement(8443..9318)
                     /locus_tag="IV228_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8718069.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9573..9657)
                     /locus_tag="IV228_002194"
     tRNA            complement(9573..9657)
                     /locus_tag="IV228_002194"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9621..9623),aa:Leu,seq:caa)
     gene            complement(9675..9746)
                     /locus_tag="IV228_002195"
     tRNA            complement(9675..9746)
                     /locus_tag="IV228_002195"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9712..9714),aa:Gln,seq:ttg)
     gene            complement(9756..9828)
                     /locus_tag="IV228_002196"
     tRNA            complement(9756..9828)
                     /locus_tag="IV228_002196"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9793..9795),aa:His,seq:gtg)
     gene            complement(9890..9974)
                     /locus_tag="IV228_002197"
     tRNA            complement(9890..9974)
                     /locus_tag="IV228_002197"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9938..9940),aa:Leu,seq:caa)
     gene            complement(9995..10065)
                     /locus_tag="IV228_002198"
     tRNA            complement(9995..10065)
                     /locus_tag="IV228_002198"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10031..10033),aa:Cys,seq:gca)
     gene            complement(10111..10182)
                     /locus_tag="IV228_002199"
     tRNA            complement(10111..10182)
                     /locus_tag="IV228_002199"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10148..10150),aa:Gln,seq:ttg)
     gene            complement(10194..10266)
                     /locus_tag="IV228_002200"
     tRNA            complement(10194..10266)
                     /locus_tag="IV228_002200"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10231..10233),aa:His,seq:gtg)
     gene            complement(10269..10342)
                     /locus_tag="IV228_002201"
     tRNA            complement(10269..10342)
                     /locus_tag="IV228_002201"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10308..10310),aa:Trp,seq:cca)
     gene            complement(10349..10429)
                     /locus_tag="IV228_002202"
     tRNA            complement(10349..10429)
                     /locus_tag="IV228_002202"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10393..10395),aa:Tyr,seq:gta)
     gene            complement(10431..10506)
                     /locus_tag="IV228_002203"
     tRNA            complement(10431..10506)
                     /locus_tag="IV228_002203"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10471..10473),aa:Phe,seq:gaa)
     gene            complement(10542..10614)
                     /locus_tag="IV228_002204"
     tRNA            complement(10542..10614)
                     /locus_tag="IV228_002204"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10579..10581),aa:Asp,seq:gtc)
     gene            complement(10624..10696)
                     /locus_tag="IV228_002205"
     tRNA            complement(10624..10696)
                     /locus_tag="IV228_002205"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10661..10663),aa:Val,seq:tac)
     gene            complement(10712..10783)
                     /locus_tag="IV228_002206"
     tRNA            complement(10712..10783)
                     /locus_tag="IV228_002206"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10748..10750),aa:Glu,seq:ttc)
     gene            complement(10798..10887)
                     /locus_tag="IV228_002207"
     tRNA            complement(10798..10887)
                     /locus_tag="IV228_002207"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10851..10853),aa:Ser,seq:gga)
     gene            complement(10927..11000)
                     /locus_tag="IV228_002208"
     tRNA            complement(10927..11000)
                     /locus_tag="IV228_002208"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10964..10966),aa:Asn,seq:gtt)
     gene            complement(11010..11125)
                     /gene="rrf"
                     /locus_tag="IV228_002209"
     rRNA            complement(11010..11125)
                     /gene="rrf"
                     /locus_tag="IV228_002209"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3561 a         2416 c         1818 g         3407 t
ORIGIN      
        1 gtatttatct ggcacaacat ataaatcgat caaaaaattg ttgcgtgttt ttttgaaaat
       61 tggagtttca acttgattac aaaatttaaa tttagaagtt gtgaaaacag cgagagacag
      121 tttaatattc aaaatataca taggctactc gaacgctgtg ttttttagta tactagtaag
      181 aaacgaaggg atagactcat tccaataagg aggatttcat gaagacaatt ctttttgctc
      241 ctgcagtatt caatttagct gagacaacac gaatgattga ggttgccaaa gctatgaaag
      301 accgttatta ctgtgtattc tttggctttt ctaaaacttt tgctcctttg atcacagaaa
      361 gtggctttga gtatcattta ctagcgccga tccttacgaa aaaacaagaa gaacaaatca
      421 taaaatttga ccagttgcgt acacttaaaa acccttttac caaagaaatg gttgcctctc
      481 gtatcactag tgaacagagt ctgattgcta agtttgagcc tcgactgatt gtgacaggtt
      541 cgaatgtgac aattttttta tcggcccgca ataaaaagat tcctttagta tatgttaaac
      601 cctatgcttt gagtgaacct ttttttcaat cagagcatgc gctgatgccc cacgagttac
      661 gtttcttcgg ccctttaagc aatccccttt ggcactggac aaaaaagcgt atagtaaata
      721 cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa agaaatgctt cctttaccaa
      781 aaacaagttt acaaatgatg gatgctgatc taaatctcat cacaacgcct aaactatttt
      841 tggaagaaga acagctgccg gaaaattatc gaatcgttgg tccaattttt gctaagctca
      901 gtacttcctt gcctgaagaa gtgctaactt ttatacagcg aaagcaacaa gaaagaaaac
      961 gtattatcta tttcgcaatg ggtagttcag gcaatccgcg tatgttacgc aaaattttgt
     1021 tcttcttaca aaaacaaccg gatctagcgg ttattgcacc tattgactat ctgcttgatg
     1081 acattcaatc tgactcagaa gatcttttaa taacagaata tctgtctagt catcttttgc
     1141 atgaattcat tgatttttct tttatccacg gcggggaagg gaccgtccaa actgcctgtg
     1201 aatccggcaa accgtttatc ggtgtgggga tgcaatttga gcaaagctgt aatattgact
     1261 attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc agtgactgaa agaaaactgc
     1321 agattgctct gcgcgaagtt tcttcaccca agattcgtca gcaggcacgc acgctgcaag
     1381 ccgtttttcc aataaatggt ccacaacaag caattcaaga aatcgaaacc ttcctgagca
     1441 accacccaat atagtttcaa gtagaaaaag cttttttaat agagactcat cagtaatttc
     1501 gctattcgag acccagacag tgaaaataca aaaaaagtaa atagtggatt gaaaagttag
     1561 agttacttaa attacggtgt ctaaaaaaca caaaatcatc cttaaaataa aagatctgac
     1621 gctatccaaa tgagcatagg tagcgtctgc catttattct tagtcaaatt ctttgacacc
     1681 tttctctggt tggatcatct caacgtttct tcaaaagaaa gaccataata attaggcaaa
     1741 tacattttct taaaatgtgt catatagatc tagaagcatc gctttgtaga gatttgtgcc
     1801 gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt accactttat ctttgataat
     1861 caacctccct cttctgttga gacaatgatc tcgcaatccg atgcatactc ctatataaac
     1921 tgccagcaat ccccacatat tgagtagatc atgtcagaat gttctatgtc gtcagcaacg
     1981 atacactaaa agtcacgttc accatgactg attgctattc ccagcacaat tatttctata
     2041 cacgtttcat gatttgaata aacactcaca tcatacatat taccattcat acaccatact
     2101 gcagccccta ctatatggtt gtaagctccc aaatctatat ttgacttgat cactttggta
     2161 gtagatctac taattctaaa tccattgctg ataaaatttc cattttttcc tccttctttc
     2221 tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa agggctgtga caaaagcctt
     2281 gtcacagtcc catattccga ataaacggtg gtcaaaagct tgcatctgcg gtaataagtt
     2341 caaccaaacg aaaaataagc caacatttca caaaaattta agaagcaatt ttcgtaaatt
     2401 tcttcttatt tcttgaagct aaccacttct gttacaacca cttttagaac atttaaagct
     2461 tcgatgtgac aaaccatcta tgctgctcga tcataaaatt gatagcaaaa attccacaaa
     2521 gtacatatgt cccccatgaa aacaaaacat tcatatgcgg tacaatgatc tttggaagaa
     2581 tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt gatttgaata tgatatgtag
     2641 ctagcatatt caatataagg tgactagccg ttaataaaaa gataaaaaca aaaaataaaa
     2701 tcagccaata cattgtttcc caccttctaa aaaccaaact tagacagtct ggacttcgtt
     2761 ttctccacga ctttgttcat ctgacagcat ctttttatca taagctttta caaatggata
     2821 atagataata aatgctatca atgtgttgat catgatcaag ataacaacac gccagtcatt
     2881 tcctgctgaa agataaccgc cgattggagc tggtaatgtc catgttggca aaacagtaaa
     2941 tctattgacc atttctaatt tgactgcaaa ataactgata atagtcaaaa ctaaaggaac
     3001 taagttgaac gggatgaaca tgtaaggatt caacatcata ggagctccaa aaattatggg
     3061 ctcattgata ttgaagatac caggaataat agaaaaacga ccaacctctt tcaagaattt
     3121 tgatttacag aagaatataa acaaaacaga taccacgatc gttgctcctg caccaccaat
     3181 tgttaccgtc cattgataaa actgttcagg agcgatatag ggtaattggg tggcagctgt
     3241 accatctgcc aatgcctttg cattctcatc caacatgatc agccacattg gacgaacaac
     3301 agcccctaca atactcatgc catggatacc aaatgaccac aacaaatgga tcaaaaacat
     3361 tggtagtaaa acaccaagca aattattccc taaaatattg gttaaaggct cgaataacga
     3421 taataaggca gcgtttacat caaagcccgc gacgcttcga atcagccaag taattgttaa
     3481 aatgactgca cctggaatca atgcctcaaa actacgggca acagcaggtg gcacttgttc
     3541 tggcattttg atagtaatat ttcgattttt aaagaaacga tatacttcca ctgcaaaaat
     3601 catagataag atccccgcaa acatcccaga tgctcccaaa gaactcatag gtaagaccca
     3661 gccaagaggt gcctcttcag gagttaaaac gtttacagga acttgtaaca taaagaacgt
     3721 tcctaaagat aatataccac ctgtcacact atctaatcca tatgatttag ctagatccga
     3781 acccataaca aaagttgcat aaatagccat caatcccatc gtcatcctaa agggtaaaac
     3841 aatcgttgcc gaataagggg caataaattc attccatcca ggaaacggta aattgataaa
     3901 gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt gaaacaaccc cattacgaat
     3961 tgctaataaa tgacgctgtg taccaatttt tgccattggt gggacaagtt tctcttcgac
     4021 ccatttcatc agcttttcca ctaaaaccac tccttttcaa taaaatatga accattcatt
     4081 gcatgacaac gcaaagaatg ttcataacta attatataat taagaaattt tatttcaata
     4141 tattctctta aataatcaaa tttattttta agaatcctga aaataagttt tctaaatcat
     4201 aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa tgattaaatc tagtgattga
     4261 aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa atatcaccct atttaacttt
     4321 tttcatcgta cactaaaatt caaaacaact atctttaatc tttacttctt ggttttgttt
     4381 taataaattt ccttcaaaaa tgcctggaga aactacaatg atcaccgtat ctttttgtgc
     4441 ttgctccaac ttcttcaaat ctataatagc taacagtgtc ccttctttta cctcctggtt
     4501 actttcaacc cgtatctgaa acggacttcc ttcgagttca actgtatcta aacccatatg
     4561 gatcagtatt tttctgccag attcactttc gatcgttatg gcatgtcggg taggaaatat
     4621 attatcgatt attcctttaa ctggcgcatg gatatatcca tcatgattcg ttatagcgta
     4681 tccaactcct atcatttttg aagaaaagac ctcgtctttt actgctgtta atggaataat
     4741 ttgaccacta gctacagctt ttagaatgtt tcttgtttta aagaaatgca ctttttatca
     4801 cctctacttt aatgctggcc aatatttttt gtttactttg atcatttcat ctagtatttt
     4861 ttttgcgact ctggcatttg gtacggtttg attcaatgta aatgcttgta aagctttttg
     4921 ataagaacct tcaaagtaag catctactag taacttctct actgcaactt gtgcctccat
     4981 caatccttta tgaaaatcac tgattggttt tcttagtgta attggctcca caccagttgc
     5041 accaacatat gccggtattt caacaactgc atctgaccgt agattcggaa tcgccccttg
     5101 attaggaacg attagcataa agcgacgacg atcatcattc aacaaggaaa tagctatatc
     5161 gacgatatat tgtccatgct ccccaaaatt aaagttcaat acttcttcca caataccgcg
     5221 gcgtatttta tcagccattt ctttggtatt tttctctcgt ccatccatga ccatattagc
     5281 acgtgtatac tctttgtcag tatgctcaac gaccatatca ggatataagt aatactctaa
     5341 gtaattattc gggatattac ttggaaaatt tttcgtgatg actgacatca tttgaaaggt
     5401 tttttgccaa gttttatctc ctatattaaa atcggcgact tgcatttctt ttgttattag
     5461 tttttcaata atctctggca taatttctcg ttgcaattct ttatcataaa tcgatgtata
     5521 ccagccaaag tggtttaaac cataatacgt aacgatccaa ttcttacgat cataaccgta
     5581 attcactgca atcgtttctt caattgaaat agtcatatca caggcattaa tcatttttac
     5641 attcggatat ttacgacgaa cagcttcaga aacgatcgac tctggatttg tataattcaa
     5701 aatccatgct ttgggggcat agtcttgtat atgatcgaca atttccaata aacctttgat
     5761 tgagcgcatc ccataagcaa atcctccgag tccacacgtt tcttgtccta ctaatccatg
     5821 tttaagtgga atcttttcgt ccttttctcg catctccaat cctccaacac gaatttgtga
     5881 gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca tccgttgcac gaatgcgaat
     5941 atttgaaaaa ccttttcttt tcagcataaa tttaacaatc aagtacatat ccatatttct
     6001 ttcttcatca atatcatata aacgtatttc agataatggc aaccgttttt cattgttcaa
     6061 aatggcttga acgattcctg gtgtatacgt actcccgcct ccagctatag tgataatctg
     6121 ttttttcatc atcatctttt cctttctatt cttctaataa tttattgaat tctgccataa
     6181 cataagtcac ttctaagccg atgataattt gataattttt ttcagttggt ttaatgatcc
     6241 cgcttatccc taattgcttg attcgctgtt cctcgaccat ggtgggatcc actacctcta
     6301 cccgtaggcg tgtattacaa ctatagacat ccaggatatt ggcttttcct ccaaacgctt
     6361 gtagcatttt tgttgccata tattcataat ttttacttgc taaccctaac tctgtttctt
     6421 ctaaggttac ttcattgcca aactctgcct tagcttcacg tcctaatgtc tgaatgtttc
     6481 tcttattgat caaataataa aaagtaacgt aatacaaggc aaaaaagaaa attccgattg
     6541 gaagaatcaa aatagcattg gtagcgattc gaaaattgat gagataatca acaatacatg
     6601 ctccgaatgt aaaaccaatt cgtatattga ataagtaaca gacaatccct gctaaaccgg
     6661 caaaaagtgc atggatcacg tacaacatag gagaaagaaa catgaaacta aattcaacag
     6721 gctcagtgat atttgcaata aatgaagttc ctgcaccgct tcccatgatt cctttgacca
     6781 tctctttatt ttctttataa gctgctttat agatagctag acatatagct ggtataccaa
     6841 acatcattac tacaaaaaaa actgataaaa acagtccagc ctgaggatct ccgttgataa
     6901 agcgtgtcat ctctcctctg aaaacttcct tgctaggaga tgtatagctt cctaaatcgt
     6961 aataaatata cgtattcaac acatggtgca gtccaaaggg gatcagcaaa cgattcaaaa
     7021 acataaatat ccccacacct aatgctccca tatcaacgag cagttttgca aaattatcaa
     7081 tcccaatttg gatgagcggc cagataaagc ccaaaaaaac agctgcgatt gtttgtagaa
     7141 tcatcaccat agtcaatggg aatttttcgc ccgcaaaata agaaaaaact actggtaatt
     7201 tttgttcttt aaagcgatta tacgtccatg ccgctactaa accggaaata attccaccaa
     7261 aaatccccat attcacttct gggtccataa tttctaagcc ttttttcaag gtagcaatcg
     7321 ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt tttgacaaaa ccgattgcaa
     7381 ctcctatagc aaataagata tccaagtttc caaaaacggc atttccagct gcaccaatta
     7441 ctgggatgtt cagcatatcg tcagctgata aacgcatgat caatcccgct aatggcatcg
     7501 ctgcaactgg aagtagcatc gctcttccaa gcttctgcac atttgattta acattcattt
     7561 ttattcctcc tctattaaat atcctcatat gttactaact caatcaaagg atcatttttc
     7621 actttttgag caaatgctga atgaattaaa aattcgactt ggtgggtacg aggatacaca
     7681 aaacttgaac catttaacaa ttcctgatcc aaatacgctg cgtgacacat gacttcgatc
     7741 gttttatctt ctctcaatat catgagtaaa cttgccaaat attcttccac actcgtattg
     7801 atttgtcgtt ttttttcatc agtttctcct acgacatcga aacaaggctc aaaataatcg
     7861 acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa ccggcaaatc atattttttt
     7921 gctaatgtaa caactacctc ctgattttca tccaaagtat gagtatgatg atgactgtct
     7981 aaatgagttg gtatgatccc cgcatgataa attttttgaa tctgagcatt ccattcctga
     8041 tacagttgat ctttgtcaat gttaaatgga tgctgataat aagataagga tctgaactct
     8101 ccgctttcga taagagtatc gacagttttg aggagtggtt tgccacacgt caatgttaaa
     8161 tggactccta cacctagcct aggcatttcc tttcttagtt tcacagcatg ctcgaaaccc
     8221 ggcatatttg ccatcaatgt agttgatgtc aaaattcctc gctgataagc atccataatc
     8281 ccataattca caccatggct atagccaaaa tcatccgaat taaaaatcac ttttcccatt
     8341 tcattctcct tcttttttct actaaaatag gcgcaaccaa ataatcatag tccatttact
     8401 atgtgtcatt tggttgcgcc tatagatagt tacgccctta ttttaataga ttcttgctag
     8461 atgaattgcc aaatagatga tttcattttc ttgaatggca agttgaaatt gctgttcgat
     8521 gatatcttta atctttagtg ctgtacaata cgctttgcta tgtttaaatg tttcagtaaa
     8581 aaaaccaata ttttctactg gcgtttcttc ctgataaatc aagcgtttaa ccaggaattt
     8641 gatatgaacc attaagcgat gataagtcag tgattcttta tcataaacgt ttgaatagga
     8701 tttttctaga tgggacaaaa tcgtggtcgt catttctact atttttaagc tctttaaact
     8761 attaggctct cctagctcag cattaatgat atgcatagtg atgaatccta cttcatttga
     8821 ccctaactcg gtttgatatc gatgattgat tcgctcgacc acttctactg ctatatcata
     8881 ttcatttgga taaaggaatc tcatttctgg caacataatt gctgtgacat ctctatcttc
     8941 tctttttcgt tgaatagcaa aataaatatg atctgtcaat gtaatataga tatttgaatc
     9001 taatggagag gagatcctag ctgttatcag ttcaatcact tcttccgcaa aatccataaa
     9061 ttcgattgga atctgcgaga ctatctcact gaatcgtttg atcttttctt tagaatctaa
     9121 tacaaataat ttgtcagcat cctcaacatc aaatggctta ctcactttag cagaaaagcc
     9181 aatccctttc cccatcgcaa ttttttctat tccatcgtca tcgatcaata cagcattatg
     9241 attcagcttt ttcatcacat acaccgttaa ttcacctcct ctatatgata aaacaaaaca
     9301 acttgcagac tccttcatac atatttttgt ctccaaacaa tttttaaaga caaaaaacga
     9361 cctaagaaag aaccctttct taggtcgtgc ctaattgaat agtagcaccc tacataattt
     9421 acaaaaatag tttaacacat aataaaagcg ttatcaatat cttttttact cgttttatga
     9481 taatacttgt atcgaattga tttggaggta attacataaa aacaaaaaaa atatagcaac
     9541 tctctataag aaagttgcta tattgataat tataccggcg gccggggtcg aaccggcacg
     9601 tcctcaacgg acactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa
     9661 tttaaaaata ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc
     9721 ttgccttacc gcttggctat accccaagaa taaagggcga atgatgggaa tcgaacccac
     9781 gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga
     9841 tgttatttaa tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc
     9901 ggggtcgaac cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat
     9961 tccgccacgc cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg
    10021 atgaaggttt tgcagacctc tgccttacca cttggctata ccgccattat actattatca
    10081 atctataaaa taaagaataa attatagaaa ctggggtagc tggattcgaa ccaacgcatg
    10141 agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa aagggcgaat
    10201 gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac
    10261 attcgccatg gcaggggcag caggaattga acccacacta acggttttgg agaccgctgt
    10321 tctaccttta aactatgccc ctataaaatg gaggagagtg gattcgaacc actgaaccct
    10381 aaggaacgga tttacagtcc gtcgcgttta gccacttcgc tactcctcca tggtggcgcg
    10441 ggacagaatc gaactgccga cacacggagc ttcaatccgt tgctctacca actgagctac
    10501 cgcgccatat tttaaaatta ttaattgaat aacaagaacg acggttccga cgggaatcga
    10561 acccgcgatc tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccagttca
    10621 ttatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
    10681 gctgagctaa acctccaaca ttcaattaaa atgacccgta cgggattcga acccgtgtta
    10741 ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa tttgctacgg
    10801 agagtaaggg attcgaaccc ttgagacagt ttggcaccat ctacatgatt tccaatcatg
    10861 ctccttcggc ctctcggaca actctccaga gtgaaagaag ctcaatactc tttctcaaaa
    10921 tgagaactcc ggcagtagga ctcgaaccta cgacatcatg attaacagtc atgcgctact
    10981 accaactgag ctatgccgga ataacatcag cgtggcgacg tcctactctc acaaggggca
    11041 acccctcact acaatcggcg ctaagaagct taacttctgt gttcggcatg gttacaggtg
    11101 tatccttctc gctatcgcca ccacactgtg gtgttatctt ttattgagta aattttgttc
    11161 actcaaaact ggatttgaag tcacataagt ttttttccga gt
//
LOCUS       DACDHL010000080        11171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDHL010000080 DACDHL010000000
VERSION     DACDHL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.080"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV228_002210"
     CDS             6..305
                     /locus_tag="IV228_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8718070.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV228_002211"
     CDS             378..1739
                     /locus_tag="IV228_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8718071.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV228_002212"
     CDS             1761..2021
                     /locus_tag="IV228_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718072.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV228_002213"
     CDS             2035..2787
                     /locus_tag="IV228_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8718073.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV228_002214"
     CDS             complement(2881..3486)
                     /locus_tag="IV228_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8718074.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV228_002215"
     CDS             complement(3539..4513)
                     /locus_tag="IV228_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8718075.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV228_002216"
     CDS             complement(4891..5670)
                     /locus_tag="IV228_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8718076.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV228_002217"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV228_002217"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8718077.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV228_002218"
     CDS             7161..8558
                     /locus_tag="IV228_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8718078.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV228_002219"
     CDS             8639..9079
                     /locus_tag="IV228_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8718079.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV228_002220"
     CDS             9227..9553
                     /locus_tag="IV228_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8718080.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV228_002221"
     CDS             9554..10303
                     /locus_tag="IV228_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718081.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV228_002222"
     CDS             complement(10383..10838)
                     /locus_tag="IV228_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8718082.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3547 a         1956 c         2294 g         3374 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt a
//
LOCUS       DACDHL010000081        11064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDHL010000081 DACDHL010000000
VERSION     DACDHL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.081"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(368..1159)
                     /gene="msrA"
                     /locus_tag="IV228_002223"
     CDS             complement(368..1159)
                     /gene="msrA"
                     /locus_tag="IV228_002223"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8718083.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1350..1985)
                     /locus_tag="IV228_002224"
     CDS             complement(1350..1985)
                     /locus_tag="IV228_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8718084.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2251..3303)
                     /locus_tag="IV228_002225"
     CDS             complement(2251..3303)
                     /locus_tag="IV228_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8718085.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3316..4254)
                     /locus_tag="IV228_002226"
     CDS             complement(3316..4254)
                     /locus_tag="IV228_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8718086.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4705..5355)
                     /locus_tag="IV228_002227"
     CDS             complement(4705..5355)
                     /locus_tag="IV228_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718087.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5455..5964)
                     /locus_tag="IV228_002228"
     CDS             complement(5455..5964)
                     /locus_tag="IV228_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8718088.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(6165..7034)
                     /locus_tag="IV228_002229"
     CDS             complement(6165..7034)
                     /locus_tag="IV228_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8718089.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(7054..8175)
                     /locus_tag="IV228_002230"
     CDS             complement(7054..8175)
                     /locus_tag="IV228_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8718090.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(8177..8572)
                     /locus_tag="IV228_002231"
     CDS             complement(8177..8572)
                     /locus_tag="IV228_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8718091.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(8591..9388)
                     /locus_tag="IV228_002232"
     CDS             complement(8591..9388)
                     /locus_tag="IV228_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8718092.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(9390..10178)
                     /locus_tag="IV228_002233"
     CDS             complement(9390..10178)
                     /locus_tag="IV228_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8718093.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(10192..10668)
                     /locus_tag="IV228_002234"
     CDS             complement(10192..10668)
                     /locus_tag="IV228_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8718094.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(10740..>11064)
                     /locus_tag="IV228_002235"
     CDS             complement(10740..>11064)
                     /locus_tag="IV228_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8718095.1"
                     /translation="LMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHI
                     SCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYD
                     YVKRA"
BASE COUNT         3498 a         2361 c         1622 g         3583 t
ORIGIN      
        1 ttatagttat tgtttgcgct tttatacgga atttagttca tcattaatgt ggaattcgat
       61 taggtgtcta ttaattatca agatgattct ctacaaaatt ccatagaaaa atcaagattc
      121 tacaattcga attctctttc acatggaaat aaaggctagt aaaaaaacag gagttgtgac
      181 aaaagacttg tcatacctcc tgtttccttg cgacttgaag ctgaatgctt tgcgccacaa
      241 ccttttcctc ggtgttctca aagcaactcc tcgaaataat tcataactgt ccaaaaatat
      301 gaaaagctat ttttgtcagt tgctcattat tttgtcggag ctgaacgctt tgcgccacaa
      361 ccttcctcta tctatgtcgc tgaaaaaata aatgaagctg atgcgaatgc ttgatctttt
      421 tatatctttt aggttgcttc tgataaaatt gttgatgatg attttcagct ggccaaaaag
      481 tctttgcttg gcgaatctct gtaacgatag gacggctaaa tcggttagat tgaatcagtt
      541 gttctcttga tttttcagcc aattgccttt gcttttcatt aacaacaaat agaattgaac
      601 gatattgttt cccccgatct tgaaattgac caaaggcatc tgtagggtct gaaatttgcc
      661 aataaatctc caacagctct tcataggtta tttgacgtgt atcaaatatg atctcaacag
      721 cttcaacatg cccagtatat ccaccactga cttgatcgta atttggtttt ttggtctctc
      781 cacctgtata accagataac actgagatta cacctggttt tgtttcaaaa ggctcaacca
      841 tacaccagaa acagccaccg gcaaaaatcg cccgttcttg gtataagggt gaagttatct
      901 tatgtttggc ataatcataa gcctttgcac tcgctgactc ccctgtgatt cttaaataaa
      961 aatccatcgc atctggtgtc aaattatctc gtagtgctag cggacgcaag ttgacctcta
     1021 gtttttctat cacctgcttt atcgacattt ttgctaggtc ttcttttgcc tcttcaaatt
     1081 gtgttctttc ccattctctt gtatttcgat ttaaaataat attataaagg tctatttcca
     1141 tctgctccac atctaacatg aagtcaccct ctttcaagtt ttcttcttat tcattcaaaa
     1201 aatgactacc tgaaatttag cacaacgcat aacaagttaa gactaacttg cttttcgctc
     1261 tcctcatctc tattcttagt cgaacacatc cacacgaatg gttacccata gagaaattct
     1321 tttacttaat cagcttcaca ctgctttttc tactttcgta gttgaaaaaa acttaaacaa
     1381 atcatcggca aatagttaaa gaaaaaactt aagcgttgaa agaagcccaa ttgcttaaag
     1441 aaggcaaact gactaatcga cggaatccgt gctaatccgt aaagacctgc aattagtaaa
     1501 ctaataactg ttaaaacaag ataaaaatgg ctaagcgtac cttgaccact ttgacgataa
     1561 agcagaacaa gtaacaatgg gaaaagtaga aagcctgcat agcctagccc agaacctata
     1621 ttatggatcc aagtcgatag attccaagag ctttcattcg tattgatact gaataaccca
     1681 gtaaaaatac aatcacctat cccatataaa ccaactgttg caacagcaag tattgcaagt
     1741 gtttttgatg ttccaataaa taggtgatac aacgcaggca acgaaagaac aaaaaaaagt
     1801 ccagatacaa ctgaccagac caagaaagca cgtctgactg gactatctac gtcaccaaac
     1861 acactaatta cggtagtcat ttgattcagt ttgggataaa aaaggccaag gatatacggt
     1921 gtcgataaat cactaatcac acctaataat aaaaaataaa aaccatattt ttgcaaaaaa
     1981 atcattttct tctcctactt tttagtgatt attcgataca tccaacaatc aaattttttc
     2041 tcattttaac acatattttt attctagttg ccttttgaac aatcaataac ataaaggtga
     2101 gtttcttcta cattttaaaa aatcgaatgg cactgaagag ttcagttgtc agcatcaatc
     2161 tcctactcaa aaaggcaaaa aaacgtaagt cagatgttgt tgacatctca cttacgcatc
     2221 cattgattga aaatttctta gtaaaatcta ctagcctaaa aatttgaaga cttcttctcg
     2281 atcatacagt ttaaatactt tctcgcataa aaattgagta attagtgccg caaaaacagg
     2341 gatgactaac caacaaagaa caacaagaaa gccatttagc cctgcatcta aagaagccaa
     2401 cggaccaaca agtccgacca cgccgaaacc agcagaagca ggcgtacctg aaatagaaaa
     2461 tagagcgaca ggaatagctg taatcactgc actaactaaa caaggaataa gaatcacagg
     2521 ttttctaaat agattaggca tcatcatctt catagcgcct aaagcaatcg caattgtcac
     2581 acctgactta ttaaccttcc aagagttcac tactaaaaca aaagtggttg cagctacgcc
     2641 catcgcagcc gcaccagcag aaacaccatc tagttggatt gccattccga ttgctactgt
     2701 tgaaatagga gagataatca aaaaggcaaa ggaacaacca atcaagatag acattaacag
     2761 cggttgtaag gtagtgaagt tattaatcaa tgatccaatt gcagaagtaa ttgctgttac
     2821 aaaagggaat aaataaaaac caagtaaacc tgcacctaca cctacaacaa tcggtgttaa
     2881 cacaatcgac actgacccaa aactatttcc aatcagtaaa atcagcccga cagcaatcga
     2941 tgctgtcagc atcgtattaa taatatcgcc tgtaccagca ccaacgtaaa cacccgtttc
     3001 acccaaatca ggtgtgaatt tgactacacc agaaccaaca aaagaagctc cggctacaac
     3061 catcattctc atcggatcaa atccaaattg taaggcaatc aaaccaccaa tgatcaaagg
     3121 tgttgctagt tgaaaaataa ctgctaactg tgcgatcgtt accgcaaagg gatacgcact
     3181 aaaatatttt aaaatggcac ctaatactgc attaggaatc aatccaataa tgattcctgt
     3241 tgctgtacca gcgaggactt tattcagaaa aattttcggt gatatttttt gtgaagtttc
     3301 cattcaatct ctcctttatt ttacgttcaa actatcttct tttgcatgga caagctcagt
     3361 caagaaatca caataaggcg tcgcaacacc atatttcttc ccttttctag aaattgcgcc
     3421 attgatataa tcgatttctg ttaatcgatg gttcttaatc aagtcttgat acattgaagg
     3481 ataatgtaac ccaattgttt ccgggtcaaa acaagattca caatgtgcaa tgacttctgg
     3541 gacatctagt tcaatcttct ctactgctgc tactttggca aactcattga caatcgtcgc
     3601 caccatttta tgagcagtcg atgtttttcc tagctcagcc atattaacgt ctaagattgt
     3661 gcatagtccg ttcattgttc cgttcacaca tgctttgcga taaatagaat agtgaatatt
     3721 gtcagaaaaa tgagcattta atcctgattc agacaatttg tctgctaatt tttttgcagc
     3781 tgcttctttt ccatcaccta aattttgtaa ttctactgaa ccacttccaa ataatttgac
     3841 ctgaccagga ccttcaagac ctgccgtcca catggtattt ccaatataga tattttccat
     3901 tggtacaaat ttctcaataa tatcttcatg cccgatacca tttaatagac ataagacctc
     3961 tgtatctttt ttgattaatg attggatatc ctgcagcatt ttttccagct gcatcgcttt
     4021 ggtaaataga ataatcagat caacttgatc ttctttttct acttcggatt gaagaacgat
     4081 tggtagtttt gcttctacct ctttaccatt aaaatttgct tgtaatccat gctctttgat
     4141 ttgttggaca tgttctgccc agccatcgat taataatact tcgttcccac tttggtgaag
     4201 cattaaccca aagcgacttc ccattgcgcc agcgcctgca attgctattt tcattgattc
     4261 ttcctccttt ttctttctat tatattcatt tgagaatcat ttcacaacct tgttttgtta
     4321 ttcttctctt atttatcatg tttatatcca ccattcaagt tattttatca gcaaatcaat
     4381 aaaaactgga atagaatccc aatttcatcg taagagaaac tagactagca ataagaaaat
     4441 agaaacattc atagaaaaaa cctcactcct ctgattataa aagagggggc attaacctcg
     4501 tgtatactaa aaccttccat tcattcgatg gatgattttc tttttgtgaa aaaatattca
     4561 ttgtagtacg atgttgcgca acacagacgg atggtattgc ttattattta ttaaaatcga
     4621 acaatacact tatttaaaaa cttctttccc agattttctt ccatttttac agttataact
     4681 agtattatca tgaaaaatag ccttttattg tctatgggaa taataattta aaattggtac
     4741 ttttttatta attagttcat cgcccaataa tctctccagt ttcttagcat ctcctgactc
     4801 aaccggaatt atccaagccc agtccaaata attttttaaa atctcatcat attttgcaaa
     4861 tatttcattg ccatattccg tccttaaaat tatattttca gtgccagaca agtgatttgt
     4921 attgagttga taaaaaagat ctccatctaa aaaaatatca ttagaagaag atttccccac
     4981 agtaatgacc attccatcag ctctatgtgt tacatacaaa taattgtatg acctcatttc
     5041 acctaatgaa tcaattattt cttgcccttt agctctaata tattgatctc tattacctat
     5101 ctcactctta tttaacaaaa gttttttcat gtcaaattta atcggctgtt ttatttttgt
     5161 aaattcgctt ctactactaa tccaatccga gattaatttt tcaacatgtc ccagagtaaa
     5221 ctctgattta ttcgctaaat actttttttg ttgtattaaa gacatctttc cccgaagctc
     5281 aaaattgagc ctacatcgat tctctatctc ttcaaaaagc atcttatctt catcattctt
     5341 atattcatca ttcataaaac caatccagct cctcgattaa gataatattt taatctcata
     5401 caaaaactag ctctcatcaa aacaaccatt gttttgattt ctcatgaaat ttctttatac
     5461 ttttctccga ttataaattc ctaacccctt gagaaaatta cgcatatatt tatctccgca
     5521 aggcttaaaa tttcgttggc cttcttttct caaaaatgcc ccgatttcgc cttttgatgg
     5581 atagatttct gcctcagcaa aaacgtctaa aatatcatca gtggtcatag acaaggcgat
     5641 ttttaatttt tttagcaaca cattgttgat atatttgatt tccttgcttt tttgcggcgt
     5701 gtctactatc ggcttatctt tttgcggacc acgcgccaag agaatcaggc catttaggaa
     5761 tgcgtcaaaa acttggttgt tgatggtttt ttcatacaca ttattttctg cattatcttt
     5821 gttcttatcc tgtactttgg tcagtaccgc ctgagcttct tctttagtca gtggtacacc
     5881 gcctagctca aaagctttaa tcaaatccgt atctttcaaa tcaatcgcat aacgcaatcg
     5941 caataaccta tcattgttat tcatttgttc ctccgaagta tttagtcttt actttcttac
     6001 gtactttttg tcttgctaag atttttagga tcaatgataa aaaccgcatc acttatgata
     6061 aggcgctggg tatcatgagt gaatgatatt gctgaataca aatagtccga ccacattaag
     6121 ttgatcggac ttcataagca ttttattgac taatactttt ttgcttaaga attgattcta
     6181 acatcttttg ctaaagttaa gcatcctata gttcctgcat tatctgctaa cttaggtgtg
     6241 acaatgtatt cttcgatatt tggcgtatcg acatagccgt taattagttc taaaaaacgt
     6301 ctttgtactt tctttttcag atgttcttgt tgcatgacac caccaccaaa aattataata
     6361 tctggtgaaa gcatcagggt cgtattacag gcacattgtg caatataatc cgcctcgatt
     6421 tcccagaacg gatcttctat agaaagatct tgtcccttgg ttcccgtacg catctcgata
     6481 gcgggtccag atgccaaccc ttcgagacaa tcatgatgaa aaggacaagt accagagaaa
     6541 gtatctcctg aatgtctctt aactaacgta tgccccattt ctggatggct aaacccttca
     6601 acaaaaatcc catcttgtat agctcctcca ccaattccag ttccaacagt gtaataaaca
     6661 acactggata aaccttttcc atgacctgaa acatactccc cgtaacaagc agcattcaca
     6721 tcggttgtcc aggcaattgg aatttcgaaa tgcttattca acgttccaac aaaatcaaaa
     6781 tttttccagg cagtttttgg agtagaggtg atatgtccat aggtctttga ttcacgatgg
     6841 atatcaatcg gtccaaatga ccctactcca atgctttcta attgttcttt atagtgatta
     6901 aagaaatcaa ttaccaaact cattgtttca ttcggtgtag ttgtaggaaa actcactctt
     6961 tctaaaacct ttaaatcatc attgccaatg gcacaaacaa atttagtccc acctgcttct
     7021 attgagccat acatttctta ctccaccgtc cttttatcta ggaattcgtt cttccctgtc
     7081 taatagtttt tcgaaaaact catgtggttc cttttggtac caataggcaa cagaagaaat
     7141 atcgtcttga cgctcataaa gcttgatgtc atcatttcct agggtttgga aagtaacacg
     7201 taaattttct ttgaaactaa tggggtcggg taaatgccag cggtacaacc catgcatcgg
     7261 taaagcatca tcgccaaaac catgaactgg atttggatta ggttttccag cactaaaata
     7321 atcccgggtg tgatctcttt tagattgaaa aggatagccc ataaatagcg tattaaaatt
     7381 ttgagcatat gccctttctc caaagttctt tttatggaaa gcccaagcgc cgccaaaata
     7441 atcttctgct cctgtagatg ttattgtagg gaaatcttca tctccatcta tataaaactt
     7501 aaattctcct tccccccacc agtatctttc caatgcgcac aaagcaaagt aggttcctac
     7561 ataataccca gtgcctttga ttccatcaat aagggtgtaa tcttttcctt ttgtcgtgat
     7621 gggttcacgg ttccatttag catgaaaata catcgcatca tccggtactt cagtcaatgc
     7681 ataattaaat gtataaaaaa tgtgcttaat aaactcagga tgttcattcg ttaaagtaat
     7741 cttagctttt tttcgaaatg gcatctcaaa atagcaattc atccctgatg ttggattcac
     7801 tacgattggt aaggaattca cttcgcatct tgtcccaaac ccattacaaa aaaaatctcc
     7861 taatggactt tctacagaag gagtggattc atcatcccaa taaatacgca aaattaaatc
     7921 acgtaagacg aaactcccag cgattgtttt gtctgtcact gtcatccaaa aatgacgtaa
     7981 aattccaggg ccttccattt ctgctaaagt aattgtctct ccagcattta tcggaacaga
     8041 aggtgaccct tttctcccaa ttcctagatg actcgctgtc attgccgctt tccctttttc
     8101 accattttta ttttctggtg aaatagttct actttcctct cccttgtgga gccatatatt
     8161 atttaaacaa ttcattttat tcgtcctcta tcctttcttc acttttttga ttattcatat
     8221 agatgatttg ttgttgtgcc tccgcaataa ttgctccaat atctaaatta tcatttccta
     8281 actgcaaagc gattgacatt gccaagggaa gattcattcc tgcaattacg tgcactctgt
     8341 cagaaatata ggtaaaaaac ttttgattta cacttcctcc agccatgtca gttaaaacaa
     8401 ttatttcgtc tgtagtatcc actaactctt taatttgttc atctatatca tcaatttctt
     8461 cgacatatgc acataagcta ctaatttgat tatgcatacc tgttaaaaac tctagtgtct
     8521 ctttaaaacc ctcagctagt attgaatggc tagcaatgat caatcttctc ataattcaaa
     8581 tccttttcct ttacgctaat attccaaatg cagccataac catcgcaaaa acaataacga
     8641 gtagaatcaa aatggtcata ctgatttttt tcttactgag tagataatat gctgcagcag
     8701 taataattac tggaattaat gttggtaaaa ttttgtccaa cattggttgg attttcatac
     8761 taacttcacc aaaagtgaat ttaagtggcg ttgaaacact gataactgaa gcaattaatg
     8821 atccaactac tgttagcccc aatactgaag cagcttcagt taaataatta atcttttcac
     8881 cgtattcagt aacaatcttc attccctgct tataaccaaa ttcaaagcct ctcattcgga
     8941 aaaagaagat taccatgatt aaaatgaacc ataaaaagat tcctacagga ttattttcta
     9001 aagccatata agccgcaatt gatcccatga tggttgggat catcgtcatc aaaagagagt
     9061 ccccaatacc agcaattgga cccatcgtac caactttaaa gtcattaatc gcatcttttg
     9121 cttctaatcc ttgcttttcc tccattgcaa cacaagcacc taaaataatg gctgccagcc
     9181 aaggctgtgt attgtaatac tgaaagtgat tatttaaagc ttcgatgtat tcatcatcat
     9241 ctttgtataa ttttcttaaa ataggtgaca atgcgtaagt cacagatgca ccttgctgtg
     9301 tttgataatt aaaggtacaa actgacatca accaacgata gttcgctttc ctaagatcct
     9361 ttttcgttac ttgatatttt tcgctcattt tattcatcct ccccaataaa cccaacatca
     9421 gaagctcccg cagaagcagt gatttgtgca tctttttcag ctctatgata cacattaaat
     9481 gctaaagcga atcctatgat cgccgctcca ataataggta ccttcaaaaa agcggctaag
     9541 aaaaatccaa caagtaaata actaatgtat cgacctgtag gcataaagcg taacaacatc
     9601 gcaataccaa cagcaggcaa catttttccg gctaatgtta gcccatccat aaaccaatta
     9661 ggtaaaacat ccaaaacgta attaacagca gactgtccta aagtaacggc aataaatact
     9721 ggaattgctg cagataaacc aaagaaaatt ggccccaacc acaaaacact attcattttc
     9781 ttaaattctc gtttattcgc atattcctgt gatttgcgaa caacaaaacc atttaagatt
     9841 ttgacaatta catctagttg cacccctaac atacctacag gtagtcctat tgctaatccg
     9901 gcttctactc caagctggct accaaatgcc gttgcaataa ttgcgcacgt tccatagtcc
     9961 ggtacagatg agccaccaaa accggcaact cctaatgaca tcaattgaac tgtaccgcca
    10021 actatcaaac ctgtttttat atcacccatc aaaattcctg tgaataatcc ccaaaaaact
    10081 gcgtaattta caaagatcat cggaatccca taataatcaa catgtttaat aaatgctaat
    10141 atagtcagca taataatttg aaaaatagta aaatgcatta tccttactcc tttatcctaa
    10201 ttttgattga atttcctgtg ccttatccgc cggaacaaat tgtgtaacaa tttctacacc
    10261 agaatccaac aattttttat acgtgctgat attttcatct gatgcatagg ctctagaaga
    10321 tagctgtaat gcattatctt taacaacagt accgccaaca attagtcggt caattctaat
    10381 accgttatct actaaatgaa ggattgtttc cggctctttc acgatgacaa atactttttc
    10441 ctgatcgtat ttcccctttt taaaattcgc aatcgctgtt tcttcagtga taattgatac
    10501 agccattcca gcaggtctag ctaaacgcat acttgatttt tggatgtcgt tatttgcgac
    10561 agaatcatcg atcaccatca ctctgtttgc tttagacatt ggtgcccatt gcgtgacaat
    10621 aatcccatgc aataaacggt tatcaactct cgacataaca atactcataa atacttcctc
    10681 ctttatcttt cattttctta caagcaatta ccatgccaaa acaaaaagaa cttgataaat
    10741 caagctcttt ttacataatc ataaacgtat ttaacctcat gaatcgggat gtcgacacta
    10801 taataatgtt caacgacact gaaagattgc ttaaaatgtg tctgaaattt ttgaaattcc
    10861 tcactcgttt cattgaaata aatttcatct tccaaagtat tttgttttac taatcgttca
    10921 attaagcaac taatgtgaac atataatccg aaacacgtat tattatcaaa tgaaattcct
    10981 agatcatttt gtaagatgta tatagcatct gaaacccgtt ctaataattt agtcggattt
    11041 aatatggtca attgctccat taaa
//
LOCUS       DACDHL010000082        11062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDHL010000082 DACDHL010000000
VERSION     DACDHL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.082"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..507
                     /locus_tag="IV228_002236"
     CDS             133..507
                     /locus_tag="IV228_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8718096.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IV228_002237"
     CDS             complement(621..1250)
                     /locus_tag="IV228_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8718097.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IV228_002238"
     tRNA            complement(1384..1456)
                     /locus_tag="IV228_002238"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IV228_002239"
     tRNA            1670..1743
                     /locus_tag="IV228_002239"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
     gene            1698..1847
                     /locus_tag="IV228_002240"
     CDS             1698..1847
                     /locus_tag="IV228_002240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718098.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2340..3668
                     /locus_tag="IV228_002241"
     CDS             2340..3668
                     /locus_tag="IV228_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8718099.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFNFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3870..4703
                     /locus_tag="IV228_002242"
     CDS             3870..4703
                     /locus_tag="IV228_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8718100.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4719..5456
                     /locus_tag="IV228_002243"
     CDS             4719..5456
                     /locus_tag="IV228_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8718101.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5611..6579)
                     /locus_tag="IV228_002244"
     CDS             complement(5611..6579)
                     /locus_tag="IV228_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8718102.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6811..7644
                     /locus_tag="IV228_002245"
     CDS             6811..7644
                     /locus_tag="IV228_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8718103.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7678..8517
                     /locus_tag="IV228_002246"
     CDS             7678..8517
                     /locus_tag="IV228_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8718104.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8543..9103
                     /locus_tag="IV228_002247"
     CDS             8543..9103
                     /locus_tag="IV228_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8718105.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9302..11023
                     /locus_tag="IV228_002248"
     CDS             9302..11023
                     /locus_tag="IV228_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8718106.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
BASE COUNT         3775 a         1821 c         2223 g         3243 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
     1801 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
     1861 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
     1921 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
     1981 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
     2041 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
     2101 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
     2161 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
     2221 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
     2281 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
     2341 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
     2401 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
     2461 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
     2521 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
     2581 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
     2641 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
     2701 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     2761 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     2821 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     2881 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     2941 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     3001 ctccaaaaga attcaatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     3061 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     3121 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     3181 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     3241 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     3301 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     3361 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     3421 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     3481 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     3541 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     3601 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     3661 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     3721 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     3781 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     3841 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     3901 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     3961 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     4021 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     4081 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     4141 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     4201 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     4261 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     4321 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     4381 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     4441 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     4501 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     4561 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     4621 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     4681 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     4741 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     4801 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     4861 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     4921 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     4981 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     5041 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     5101 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     5161 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     5221 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     5281 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     5341 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     5401 tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat aaataaaaaa
     5461 gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga aaattgtttc
     5521 tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc acagcctctc
     5581 tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa tcaattcttc
     5641 acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt gactattaat
     5701 actgttacag aaaataatcc ccattttttc tagttctttt cccccgcctt cataatcata
     5761 aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga ttttttggac
     5821 aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac ctaaagcttc
     5881 gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc cttgcatccc
     5941 agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca gtcgctttgt
     6001 ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga tgggacccaa
     6061 tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga
     6121 atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt cacaacttgc
     6181 gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat tacttgaacg
     6241 cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc
     6301 agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac ctgatgcata
     6361 agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg
     6421 aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga tttctgcatc
     6481 taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt ctttcatggc
     6541 gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt ccttacgaat
     6601 aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga
     6661 agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact
     6721 caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc
     6781 caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa
     6841 gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta
     6901 cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat
     6961 tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc
     7021 ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta
     7081 gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc
     7141 tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt
     7201 gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt
     7261 gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca
     7321 cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt
     7381 tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa
     7441 aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa
     7501 gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat
     7561 gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat
     7621 ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg
     7681 atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg
     7741 gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca
     7801 acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat
     7861 gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt
     7921 gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt
     7981 tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt
     8041 atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc
     8101 ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa
     8161 gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat
     8221 cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa
     8281 ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa
     8341 gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt
     8401 ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa
     8461 tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca
     8521 atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt
     8581 ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc
     8641 tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc
     8701 taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga
     8761 agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc
     8821 actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga
     8881 tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc
     8941 tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc
     9001 tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa
     9061 gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt
     9121 ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa
     9181 aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt
     9241 atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata
     9301 aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt
     9361 ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa
     9421 acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat
     9481 gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat
     9541 atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga
     9601 aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca
     9661 aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga
     9721 acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt
     9781 cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt
     9841 gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc
     9901 ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa
     9961 aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag
    10021 tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga
    10081 agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga
    10141 agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga
    10201 tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc
    10261 agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac
    10321 atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga
    10381 agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat
    10441 tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga
    10501 aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc
    10561 gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa
    10621 acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg
    10681 tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg
    10741 tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt
    10801 agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca
    10861 cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta
    10921 tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa
    10981 acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa
    11041 accagcaaga gcaagacgga tt
//
LOCUS       DACDHL010000083        10746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDHL010000083 DACDHL010000000
VERSION     DACDHL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.083"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(23..1807)
                     /gene="pyk"
                     /locus_tag="IV228_002249"
     CDS             complement(23..1807)
                     /gene="pyk"
                     /locus_tag="IV228_002249"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8718107.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(1881..2843)
                     /gene="pfkA"
                     /locus_tag="IV228_002250"
     CDS             complement(1881..2843)
                     /gene="pfkA"
                     /locus_tag="IV228_002250"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8718108.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(3011..6325)
                     /gene="dnaE"
                     /locus_tag="IV228_002251"
     CDS             complement(3011..6325)
                     /gene="dnaE"
                     /locus_tag="IV228_002251"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8718109.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            6556..6741
                     /locus_tag="IV228_002252"
     CDS             6556..6741
                     /locus_tag="IV228_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8718110.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(6803..7999)
                     /locus_tag="IV228_002253"
     CDS             complement(6803..7999)
                     /locus_tag="IV228_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8718111.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(8169..9605)
                     /locus_tag="IV228_002254"
     CDS             complement(8169..9605)
                     /locus_tag="IV228_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8718112.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            9898..10707
                     /locus_tag="IV228_002255"
     CDS             9898..10707
                     /locus_tag="IV228_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8718113.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3208 a         2250 c         1917 g         3371 t
ORIGIN      
        1 ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa acgatgccgc
       61 gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg cctttagcac
      121 caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa gtcaatccgc
      181 cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct ttgtcagttg
      241 atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca gcagatgaag
      301 caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct aataatttag
      361 aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact ggtacaccag
      421 cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct tttttagctg
      481 ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg taaacgcccc
      541 agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag atatctgcat
      601 ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca gcaacgattg
      661 ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt tcagttacat
      721 ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca ttttcagctt
      781 cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg tttgcagatt
      841 caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg tcagaagctt
      901 ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta gcagtgataa
      961 ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt ggtactagtt
     1021 cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca gaaactttga
     1081 tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag atttgaacat
     1141 gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt gctttacgta
     1201 caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata tcgtcagcat
     1261 cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc cctttacgtg
     1321 atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct tcgtcttttt
     1381 caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca acgtgaacat
     1441 cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca tgttcaggat
     1501 ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca cgaccaaaat
     1561 cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta tctaaaagaa
     1621 taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata cgtgataatt
     1681 gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca gattcaatca
     1741 attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg attttggttt
     1801 ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac acagcttaaa
     1861 tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata tagtgataag
     1921 tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac gatttcgttg
     1981 tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac agcgtaagca
     2041 ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg gacaacgtgt
     2101 cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc agcgaattca
     2161 tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc gcgatcgcgg
     2221 ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg gatgatgatt
     2281 tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt gcgtcccatt
     2341 acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg gtcaattgat
     2401 tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc aggaatatca
     2461 ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa agccattgcg
     2521 ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa ttttttcaat
     2581 tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg tgcagaatac
     2641 aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag acgacgaata
     2701 tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag tccttcgtaa
     2761 atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc tccgccactg
     2821 gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact ttatcctaag
     2881 tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta acattatttt
     2941 tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa gtagtatttt
     3001 ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata agttgcccct
     3061 ttatactctt gctgtcatcc acccaataag catctgacag aactactttt cgttgcgttt
     3121 tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc acttgtaatt
     3181 gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa gtttgtgaag
     3241 gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt tgcagttcct
     3301 gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca acggcaaaca
     3361 aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct ccactactgt
     3421 cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct ctaacttcct
     3481 tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc agctgaactt
     3541 gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa taaataccta
     3601 agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact tcttcttctt
     3661 ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata ttttggattt
     3721 ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca aaaactccta
     3781 ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc cgtatgagga
     3841 attgatccac tgactggaac gggccatttt gttttctttc gtttaatata tcttctacaa
     3901 aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc ttattgacca
     3961 actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt ttatttttac
     4021 gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa ttcatcaggc
     4081 tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat cccacgaatg
     4141 aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg atatattcat
     4201 atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat acaaaatgcg
     4261 acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc agaatatctg
     4321 actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt tcctgataga
     4381 cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg tagcgaatgg
     4441 cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg cctggtcgat
     4501 acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt cgtaaaacgt
     4561 tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt tgaaacaaga
     4621 gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac ccttctactc
     4681 gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct aaaaagtcca
     4741 ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga aaagcttcat
     4801 cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata actacacctg
     4861 ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt ttgaataaaa
     4921 gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt ttataagctt
     4981 gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt gcttcacttt
     5041 gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc attgtgccaa
     5101 atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa ttcaatactt
     5161 cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag cgttcggggt
     5221 ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg atttctaaca
     5281 cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg attttgtttc
     5341 tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat cccatttgat
     5401 ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag tcagtgattc
     5461 ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt tttccattag
     5521 gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa atcgattcag
     5581 ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat cgtttgatca
     5641 tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca gataaatttt
     5701 ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca aaaagttctt
     5761 cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct tttatcacat
     5821 actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata taaaaatttc
     5881 tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc tctttattga
     5941 aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg gtttctgtca
     6001 tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc atcagctgct
     6061 gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct tcataagtat
     6121 aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata cgatagaatt
     6181 caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag ccttgttttt
     6241 ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa taagaagtaa
     6301 ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt aaaattcact
     6361 agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga agttgattat
     6421 tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag aactcaaatt
     6481 ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa ttaagataga
     6541 aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt tctaggtcaa
     6601 gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac tttgacaaca
     6661 atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc gccaagaaaa
     6721 gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca agctcttttt
     6781 tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga aagttgaata
     6841 aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca gcactgtaat
     6901 tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca aagttgctaa
     6961 acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc ttactgatca
     7021 gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag cgaagtatac
     7081 ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat gataaaatga
     7141 gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct agtttcggtg
     7201 aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca aaaaggatat
     7261 tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg atcgtagtga
     7321 ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag agatgatcca
     7381 tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga tggaaatctt
     7441 ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct gtgatcaaga
     7501 atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt ccgatcagat
     7561 ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt tctctaggtg
     7621 cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc ccattccaaa
     7681 accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc attgttaatg
     7741 tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca gctagatttc
     7801 cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct agtcccgaaa
     7861 acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt atgaatggca
     7921 taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc actaaatttt
     7981 ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata cctattttcg
     8041 aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg tcattttata
     8101 tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt ccaatccctt
     8161 ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat atcaacgatc
     8221 cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat tgtatagaaa
     8281 ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa gatcgatgca
     8341 aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa agaagccatc
     8401 atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac aatggcgatc
     8461 agtggagaga ataaactact taatgcgaat agcactgcca ctactactga agtcaaacca
     8521 gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt attcgatgta
     8581 ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt atccattttt
     8641 gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa aatacctgtg
     8701 cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa actgaatgcg
     8761 atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga ttgcatgcct
     8821 tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt acctaacgaa
     8881 ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc aaggactgtg
     8941 gtcgctacga taccgataag aatagcccca cggacatttt ttacaactaa aatggctgtc
     9001 agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc taacgcaggg
     9061 acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac agagctcgaa
     9121 atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc gacatacgca
     9181 acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat cgcccgaata
     9241 atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca aataaacacc
     9301 attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa agtgaaaaat
     9361 gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa tcccattacc
     9421 agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa gggcatacct
     9481 gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa gaatgtcgtt
     9541 actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc gaaaaacttt
     9601 tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat aataaaaaat
     9661 aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt cattcgcttt
     9721 tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt tagcttatgt
     9781 ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt gctcttgtta
     9841 attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg acccttattg
     9901 aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga ttcgatgatt
     9961 acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca tgtcagtatt
    10021 gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga attaacaaca
    10081 ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg ggatggtgaa
    10141 agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa aaagcagcta
    10201 aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag ttttgatttc
    10261 tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct ttctcctaaa
    10321 aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt tgcaggagct
    10381 gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac atggggtgga
    10441 cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg cgtgcaagag
    10501 attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga cgagcataat
    10561 gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg tactgaccaa
    10621 ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg tttagctgtt
    10681 tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa agaggttgtg
    10741 acaatt
//
LOCUS       DACDHL010000084        10564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDHL010000084 DACDHL010000000
VERSION     DACDHL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.084"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IV228_002256"
     CDS             23..646
                     /locus_tag="IV228_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718114.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IV228_002257"
     CDS             1096..2145
                     /locus_tag="IV228_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8718115.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IV228_002258"
     CDS             2142..3023
                     /locus_tag="IV228_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8718116.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IV228_002259"
     CDS             3026..3796
                     /locus_tag="IV228_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8718117.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IV228_002260"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IV228_002260"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8718118.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IV228_002261"
     CDS             5107..6270
                     /locus_tag="IV228_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8718119.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IV228_002262"
     CDS             6387..6797
                     /locus_tag="IV228_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8718120.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IV228_002263"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IV228_002263"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8718121.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IV228_002264"
     CDS             9337..9654
                     /locus_tag="IV228_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718122.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IV228_002265"
     CDS             9814..10050
                     /locus_tag="IV228_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8718123.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IV228_002266"
     CDS             10047..10445
                     /locus_tag="IV228_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8718124.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3683 a         1770 c         2234 g         2877 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taat
//
LOCUS       DACDHL010000085        10406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDHL010000085 DACDHL010000000
VERSION     DACDHL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.085"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..843
                     /locus_tag="IV228_002267"
     CDS             <1..843
                     /locus_tag="IV228_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8718125.1"
                     /translation="ETSPYSVFKWGDTAGQGLSYVAAKANIKVTNKDGSVVLKENTDY
                     KIQNSENGFVIDFIVNNGQVSDTVASLHGQDLQMTYNMYVNDSAAVANPLTNSVDFVY
                     SNNPFNQEEHHEKTKADAVTYGAKFLKADGGLFGTGIKATPLEGAEFAVKNAEGKYYG
                     GLVDTDKDGVKEAVWVDDVANAAILKSDKEGHFEITGLTEGEYSLEETKAPTGYQKLT
                     EDIAFKVNKNSFKEENRITVKNNQKAAIPLTGSNGFQTYVLISCLLLGAGALSAVVYF
                     KKKA"
     gene            856..1611
                     /locus_tag="IV228_002268"
     CDS             856..1611
                     /locus_tag="IV228_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8718126.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPSVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            1608..2432
                     /locus_tag="IV228_002269"
     CDS             1608..2432
                     /locus_tag="IV228_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718127.1"
                     /translation="MNKRKWLFVCVAALFSTAAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQPVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            2722..4833
                     /locus_tag="IV228_002270"
     CDS             2722..4833
                     /locus_tag="IV228_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8718128.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDNKNFDMVSDGEKYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(4871..6928)
                     /locus_tag="IV228_002271"
     CDS             complement(4871..6928)
                     /locus_tag="IV228_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8718129.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(7042..7254)
                     /locus_tag="IV228_002272"
     CDS             complement(7042..7254)
                     /locus_tag="IV228_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718130.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            7441..7908
                     /locus_tag="IV228_002273"
     CDS             7441..7908
                     /locus_tag="IV228_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718131.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFI
                     NKPDSLFIVVPYEKDGVKKIVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7905..8147
                     /locus_tag="IV228_002274"
     CDS             7905..8147
                     /locus_tag="IV228_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718132.1"
                     /translation="MIASLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEISEH"
     gene            8165..8467
                     /locus_tag="IV228_002275"
     CDS             8165..8467
                     /locus_tag="IV228_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718133.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            8539..>10406
                     /locus_tag="IV228_002276"
     CDS             8539..>10406
                     /locus_tag="IV228_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718134.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEA"
BASE COUNT         3699 a         1633 c         2028 g         3046 t
ORIGIN      
        1 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaagg actttcttat
       61 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
      121 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
      181 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
      241 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
      301 gtttatagca acaatccatt caatcaggaa gaacaccatg aaaaaacgaa agccgacgct
      361 gtgacttatg gcgcaaaatt cttaaaagct gatggcggac tatttggaac aggaatcaaa
      421 gcaacaccgc ttgaaggcgc agaatttgct gttaaaaacg cagaaggaaa atattatggt
      481 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
      541 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
      601 ggtgaatatt cactggaaga aacaaaagca ccaactggtt accaaaaatt aacagaagat
      661 attgcattta aagtaaacaa aaactcattt aaagaggaaa atcgaatcac tgttaaaaat
      721 aatcaaaaag cagctattcc attaacaggt agtaatggtt tccaaacgta tgtattgatc
      781 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
      841 tagaaggatc agctaatgat tattcgtaag cccccaaaaa gaagagtcat gcacaaactg
      901 atgatatttt tttggttgct gtcggcagta gcagtctatt taattccatc tgtggaaaca
      961 aatgtagcga aatttcaaca acagcaaaga attttagcca ttgaaaatct tgaaaaaaca
     1021 gattcttctt tggatgttgc gaaaatagag ttaggcgatc ctgttggtgt tcttacaatt
     1081 ccaagtattt ctttaaaact accaatttat gacggaactt ccgacaaaat tttagagaat
     1141 ggtgtaggga ttacggaagg aactggcgat attacaggag gaaacggtaa aaatccttta
     1201 atcgcggggc acagtggact ttataaagat aatcttttcg atgatttgcc ttcagtaaaa
     1261 aaagggcaaa aattctttat taaggtaaat gaagaacaac atgcctatca aatttatcga
     1321 attgaagaag ttaaagcaga agaattacaa aaaaattatt tagattatct catgcctgaa
     1381 aaagaccaag ataggatcac gttaatgaca tgcacaccaa aaggtgtcaa ctcacaccgc
     1441 tttttagtgt atggtgaacg agtttccttc agtaaagaag atttaaaact acagaaaaaa
     1501 gcaaaaaata ggaaatcaat ttcattggat tggcgattag cactgctatt cgcttttttt
     1561 tgcgttccta ttatttttat ttataaaaaa ataaggagga gatcggaatg aataagagaa
     1621 aatggctgtt tgtttgtgta gcagctctct tttctactgc agcttattcg caactgtctt
     1681 tcgctgacag cagtgatagt agtaccactc aaacaataga taattcaatt actgcaccaa
     1741 gcgaaacgga cagttcggag aatactacag aagaaacggc agattcaaat ataagcttta
     1801 ctgaatcaac gaatccaacg tcagaaagtt cttcagagac ttcagaaaag ccgcaagacg
     1861 tagaagatac gcaagtgatc aagcaagcca ttcatattca aaaaattatt tcttcgaatc
     1921 aaatcaacaa tcaaggcagt caagtagccc agcaagaagg tatcaatggc gcaaagttta
     1981 ttgtctacga tatcacgaat atcttagaag aaatgtcaca agatgataat gagaaaaatc
     2041 aacctgtaga aataattgag tcaaaattga aagatcgagc aaaaaaactt tcatacgacc
     2101 agttgaaaaa agtggctgaa ggagaaacca aaacgattga tgatcaggaa ggcgtgattg
     2161 aattttccat tgaagtacct gcaaacaaaa aacaagcata ctatatcgtt aatgaatcaa
     2221 gccctgaaaa catttcaaac agtgaagata ttgttttatt gacacctgtt tcaaatgaaa
     2281 aaggtgaatt tttgacggat gtttggttat accctaaatc agagaaatca gagccgaaag
     2341 aagaaattaa aaaagttgtg tcaactggtg taaagaagaa cttttttgaa aattgttggg
     2401 aattttttgc tcaattattt tttgataatt aggatgaaat gatgaaaata ataagaatat
     2461 tagtttttaa tctatccttt ttagccgcaa cattattgtg ttgcggttta attttttctc
     2521 aattatcaat ttatccaaga tatcaacgat tgttcattca ttttctaaac attcaatgtg
     2581 cttccatgcc attagaggtg attaaaaagc ttttgatcag tccaattttt atggttagtt
     2641 tggttcttct tacaggattg gtaacgtggg tattaggaaa aaaagaatga aaagcttacg
     2701 atcgtttaaa aaaaatatta gatggagggc attatggaaa ataaaattca ttgcactacg
     2761 ggaagagtaa ttactttagt gttgtttttg ttccttcttc tttttgcgag gggaactgca
     2821 gtccaggcct tagaaaaagt tgatccgagt caaatcaaag aaaataataa tctggtgctt
     2881 tatcatgata gtgattcatt gagaaatatc gaagtaactg ggatcaatgg atttaagata
     2941 ctagatgagt attcaactaa aaaacaaaaa gtacttgctt ggccaaaagt caaagatttg
     3001 aaaagtacgt caaaaatcgt tgcccattat caaaaagtag gtacttatct aggaaaagaa
     3061 attgattgtg acgtgacttt ttctaatttt gtagcgcacg ctggggttaa ccaagacgga
     3121 ttagctaaaa caattaatgg caaaaagtat tataattatt tgctctttga taaaaatatt
     3181 tatcgaggat ttttcatgca gtcaatcaat gtcatgaatg caaaactaga atttaagtat
     3241 gcaggaacaa aaacaaaagt agctttgagt gaaccacagc cagaaattca atcaaaaatg
     3301 agatcgatga gagcagctgg tattgattta aacgatacat ttctatccat ttatagttta
     3361 aatggacagt atcagcaacg tgtgtcttgg tataatgaca ataatgaatc tgtcggtgaa
     3421 cgtaatattg atatgagaga atttgttggg tatgaaaaaa tgggtagctt accttatttt
     3481 gtcacaacag atacagcatg tgcagaatac aaagctcctg cgttatcaac aaacaattta
     3541 acttcaaaag tagtgggagg acgcgcagaa aaggcttata gctcgaatga tcatttcacc
     3601 gatgttgtag gagctgatac ttatcacaga agtggtgtaa cgtatacgct tcaaggcgct
     3661 tccccaacat tcatgattgg cgcaaatacg aatagtatga tgtttagctt tgatactgca
     3721 ttgctatgga caccacaacc atcgaagcct acaaaagaag tgtttaacaa agctaatact
     3781 gaagaggcag cacacaatat tgacaaaaaa gtgattccac aaggatcaga tgtttactat
     3841 catattcacc aaaagtttga tgcattaaca gtcaacacaa tgaacaaata caaatcattt
     3901 aaaatcactg atacctttga caacaaaaat tttgatatgg tatcggatgg ggaaaaatat
     3961 gatggcgcat tgtggtatgt tgaagcgaat ggtacttgga aaaaagctga tacaggagga
     4021 aaattaactg tatcaggtaa tacagtcact tatactgctt ctgacagctt tttagctaac
     4081 gctgcaaatt acggtaggga ttatattttg cagctccata tgagagcaaa aaatgatgca
     4141 aaaggaattg caccaaatgt ggctactgta acgtttaaca atgatccgaa caaaggaaca
     4201 caaaaaacaa atacagtaga aaactatttc tctcttaaac caaataaaaa agtggaacaa
     4261 gatgggaaag atgttgatgg tcgaaatgat ggtaaaaaag gaacgcctac agctccttta
     4321 aatgcaggat ctgaagtaca atatctagtc acacaaaaat ggcatacaaa aggtgtagac
     4381 attgcctcag atcattacaa gcaatttagt attcaagatc cagtggaaga tcgtttgacg
     4441 tataaagaag gttctgcgcg agtgatagat atgagtacag gaaaagatat tacttcagaa
     4501 ggtacactaa cctatgatag caattcaaga acgctgaagt gggaagcttc cgctgatttc
     4561 ttaagtaaaa atcttttaga tggacgagaa attcaactga tatttacagc taaaactcct
     4621 ttgaatgaag aattggatat cgacaaccaa gcaaaagttt cagtagaaag tattgagaaa
     4681 gcaactaatg tagtaacgat tggggtaaaa cctaacttac ctcagatcat tgtgccaaaa
     4741 actggatcga atcatttagt gctaactgtt attggaagta tattatttct tactttagca
     4801 tcattgggct atgtagtttt aaaactcaaa taaatataaa aacaaggctg agaatcagcc
     4861 ttgttttctt ttatttgaag attggattcg cactaataaa tacattatct acgccaattc
     4921 taccagcaga gtcaactaca aaattatgac ctaatccgag aatactttgg ttattataaa
     4981 gtaaatttgc attatgggca gggctatttt tccatcctac catggcatcg atagcaccat
     5041 cgtctgataa aaggtcatca atatacagcc cagtttgact tatgttttct cccttagcac
     5101 cgtagcagcc taagctttcc aataaatctt ggaaagtacc accaccaggg cgatagtgac
     5161 tgaagctgac ttgtaattct ttagctcgaa tatctgcagc ctgttgaaca atatcattga
     5221 attgcattgg ggcaacacca ttttgttggc gataatcatt taaccaagca agttcttttt
     5281 gacgcataaa gttacgtaat tcgtctaatg aaatcttttt atatttgaat gtgattgttt
     5341 tattttgatc gacgttatta atggatcgtt ggccgttttt atccacggta acggtaggat
     5401 cgtcatttct gcacaagaaa taattttgat cagtcatgtt tttccaagga gtagcaaagc
     5461 tatccccttc gtatactgtt tttgattcac tagagagaac agcgttggta tcagcattta
     5521 catgattaac agtcacatta aatttacgag tgtagttgaa aatataattt tgactttcag
     5581 tcacattgtt taaggttact gaatttgttg gttctacccg atcgtctaaa acatatcctt
     5641 gaatagaagg agcagttaaa gtgacactgt caccgtgagt gacattgact atctgttctt
     5701 tcaaaatatt aactcgtcct tcgtttttgt agttgttatt gcacaacaaa ttgatttgat
     5761 gcacttgatt ataattgaat gtgattgttg tattacctgt gattgttttg ctttgagtat
     5821 cattaccaat cactttgtag ctaaaattat ctttagcgcc ttcgctatat ttaaatgctt
     5881 ttgccttagc agtgtaagta gaaccagaag ttacttttgc ttgatctgta gcaatcactt
     5941 gacctgtttg attgtcaata tggttaatag taaccgtata ttcttttggt gtttcaggtt
     6001 tgtgttgcaa ccctttaaat gcagcatcaa gactattttt agcattattt acttgggctt
     6061 gagaagcctt agaatcgtct agtacttttt tagcattgga taaagcagtc tgaaaattat
     6121 tccaagattc ttctgtaaaa tctcctttag cagttgattt tactcggttg tataaagctt
     6181 gtaattcagc tttattcaca gtaggaactg gttttttctt taatcctttg taagcagaat
     6241 ctaatttatt tttggcacta tccacttgtt tttgagtcgc ttttgagtca tttaacactt
     6301 tcttagcatt gtttaaagcg gtttggaatg cgttccaggt attgtcggta tagtcacctt
     6361 ttttagttcc cttaacgctc gtataaagag cttctaaagc tgatttattc actttgggtt
     6421 tagaggtgtc agcgccatac caaccgatac cttcgtattt ccagccatag tctttggtta
     6481 accattgtac ttctttccaa cttgcagtgt agtgatgtga gccagatttt acaccaggaa
     6541 ggaaaaggcg gtagatcggt ttattatttt tttcggcaga acgccaggca ggcccttcat
     6601 aagtccagcc gtaattttta gtcaaccatt tcacttcatc ccaactagct gtgtaatggt
     6661 gatcacgaag acctttattg tacaaacgat agacaagatg gccaccgcta gtcggtgctt
     6721 cccaggcggg gccttcataa tttccccatt ttatctttac tagatggtca cgttcttttt
     6781 tgctagaggt gtaaaaatgt tcatgattat ttggattgta aaggcgatac atgacgttcg
     6841 ttgaagcagc gtcaacagat ccagcaagac taagtgaggc agcaaatgtt ccaatagcta
     6901 aaacagtttt tggattaaat aatttcattt tttcaactcc taaatttaat taaaaatgtt
     6961 tacatgatta ttatatatcg gctaaccgat atagtcaata aaaaataaga aaagccttat
     7021 ataattttgg cttttcttat tttatttgtt agttgaagta ttaatatcaa cgatttcttc
     7081 agccaattta tttaatttca tagcatttat gaatctcatg ttgtctatac tagagcgttt
     7141 gttggcaagg tcactgactg tagttaaggg tacgccagat tctatggata tagaacgaac
     7201 tgatttacca ctatcaaaca gcaaccattc gattttcttt ttatcagcgt acatttcaat
     7261 gctccttctt gttttttctt tattatagct cttttttgta ataaatcaca atatcaagag
     7321 aaaaattttc tatagaaagg tggattttta tgagtattcg ttctccgtgt atccaaatga
     7381 ggtttgttgc tactttcgtt atttgatata ctaaaaataa acaacgaaag aaggaataaa
     7441 atgaagaaaa agcaactatg gtttgtctct atgtttgcaa tattttttat tattttaagt
     7501 ggatgttcaa gtaataagaa atcggaagct aaagaagata atagtttatt gcaaccagga
     7561 actgtttgga aagaagaagt aggtggtctt attcataatt taaaaattat agatgatact
     7621 acctgggaat attctgaaag tgtatggcat ccagatcctg tacaaataac agttaagaga
     7681 caaaaagact atgaagggtt agaaagatac aaaatagtag attctgcagg tatcagaaaa
     7741 tttataaaca aacccgattc tttatttatc gttgttcctt acgagaaaga tggcgtaaaa
     7801 aaaattgttt ttcttggatc tagtaaggat gaaaagcgaa cgaaagaaaa attgattcat
     7861 gatggatcac aatcaaataa atacaaatta caaaagacta gcgagtgatt gctagtcttt
     7921 ttatttaccc taaaggagga atcatagtgg caaaagcgaa aacgccattt gaaaaagccg
     7981 cgtctaaaat cttagaacgt cgcggtgtat ctgaaacaca gtggaaacaa gaagtccttg
     8041 aaaaagcaca atttgaccta ttacggggaa aagacaagga cttagaagga tatatctatc
     8101 aacgtgaacg tgaaaagctt gttttcgatg aaattagtga acactaaaag gagcgaacaa
     8161 ttttatgttt gaatcaatta caatgaattt tttaacacaa ccgaaagtag tagctgcggt
     8221 tgatattttt agagcattac aaactgccgc aactagtttg aaaaataatc tgatgatttt
     8281 tgctgctgtt gcggcagtgg cttccattgc tcttggaggt attttcttct tctttgggcg
     8341 aagaggagct gaaaacggga agacacacgt aggttatgta ttgttggggg taggagttat
     8401 tttagctgcc gcttctattg tagtttcaat gttctcaatg tttggacaaa ccccacaagg
     8461 actataaaaa aaacaagtag aaggagtaaa aagtacgtgg ctacttttta ctcctttttt
     8521 tatgaaagga gaatattcat gttttttacg atttcaggaa atgcaaaatt ggataaagag
     8581 ttaataagaa aagcaacgaa gaaagtggga aagcgtactt cctttttacc gaaaacaatt
     8641 aaatattctg tcattgaaac ggaagaacag ctacatgtgt taggacaagt gttagaacaa
     8701 gagaatcaat cttctgttaa attgactctc acgttacatg ataaagaagg aaaaatagaa
     8761 aaggctggca cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttttat
     8821 cctacattga tggatttaat ccgccttgat gtgtttaaag atacggatga aactatgagt
     8881 gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc
     8941 gaccaaagaa aagtgaatca agtgagtgaa gtcgaagtaa atgaagaagt accatgggaa
     9001 cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa
     9061 gctgatattc cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg
     9121 gaggtagatc cctttgaaga aacaatggag gatgaagaga cggacgaagt agaagttgat
     9181 ccagttgaag ctaatgaaga gtcaacatgg actgaagggg attctcagga atatattgaa
     9241 ccacttatca gtgaagaagt agaaaaaaac gaaacagaga tacaaaaggc aactgtattt
     9301 gattatattt tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta
     9361 tttttaaatg agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag
     9421 agtaaagaag aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc
     9481 caatcaacaa attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt
     9541 caacaagaaa cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag
     9601 aaagtaaaag aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa
     9661 atcaaagagc ggtacgaaaa gaaacgcgac aatgcttatt ccttgtgtgt agaaaagatc
     9721 aacaatttta ctaagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa
     9781 gaacgtatta aagaacgaaa agaagagttc taccgcggtt tacgtacaga taagctaaag
     9841 attagcaaag aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt
     9901 atggaaaaac acgaacagat ccagcaagaa ttacaagtac aaattccaaa atggaaaaaa
     9961 gagtatgaaa ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa
    10021 aaagagcgtg aacaattaga aataagaaaa cgggaacagg atcttgcgga acaaaaaatg
    10081 gcgatagaaa aacaacggtt agagcaagaa aaggaaaaag aagcagctgc tcgtaaggaa
    10141 cgtcaagaac aaatggatac attgcgtcaa gcacagttga attatctagc acaacctcaa
    10201 ccagtagctg ctgttcaaac gccagtgaac aaccaagcaa atttaactcc ttcaccccag
    10261 cgttccttta acggatggat ggtgggatgt attgcaagtt taagcttgct tctaggtgga
    10321 ggaacagtat ttgcttttaa tcatatcaat caatcttcac aggctgattc tgtagccaca
    10381 acggcgtcaa tcaaggaaga agccca
//
LOCUS       DACDHL010000086        10316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDHL010000086 DACDHL010000000
VERSION     DACDHL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.086"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(70..903)
                     /locus_tag="IV228_002277"
     CDS             complement(70..903)
                     /locus_tag="IV228_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8718135.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1207..3372)
                     /locus_tag="IV228_002278"
     CDS             complement(1207..3372)
                     /locus_tag="IV228_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8718136.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3632..5926
                     /locus_tag="IV228_002279"
     CDS             3632..5926
                     /locus_tag="IV228_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8718137.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5919..6584
                     /gene="pgmB"
                     /locus_tag="IV228_002280"
     CDS             5919..6584
                     /gene="pgmB"
                     /locus_tag="IV228_002280"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8718138.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6577..7584
                     /locus_tag="IV228_002281"
     CDS             6577..7584
                     /locus_tag="IV228_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8718139.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7606..8625
                     /locus_tag="IV228_002282"
     CDS             7606..8625
                     /locus_tag="IV228_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8718140.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8906..10273)
                     /gene="nhaC"
                     /locus_tag="IV228_002283"
     CDS             complement(8906..10273)
                     /gene="nhaC"
                     /locus_tag="IV228_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8718141.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3197 a         2241 c         1909 g         2969 t
ORIGIN      
        1 tctccaaacg gtataatcgt ttggagagtt ttattttaat cagttttttt atgttatatg
       61 ttatattttt tattcgttca catctaattc gatttccacc ccgtagtgat cactgatgat
      121 tggactatcg atcccatcaa aaatccgttg atatttttta acctgcattc cttcagggac
      181 gaaaataaaa tctatacgca atttttctgt attttgttcc caaccatcta ttttcttttc
      241 cacagtagct gcttttcctt tttcattcgc gacaacatac gaatcttgaa gaagaaggca
      301 actttctctt acagtataat aaccttgaga gtctactgga ttattgaaat cacctaggaa
      361 aaaaagtggt acgcgagttt ccaaaaagta gttttccaag tttttccact caaaataaaa
      421 gccacttttt ttatctgtcc accaagaaaa gtggcagctc gagacaaaaa tgtcctgatc
      481 atctatcttt gttttcccaa tcaagatctt gcgtgtctga taatcttctt tcttttgaaa
      541 ttgggagacg gtaaggacct gactttccaa tgtttttttc gtaagcagtg catttccttc
      601 ttcatattta tcataaccga tgtggctcat ttcccaactc caaaaatact cttttccgtg
      661 ttcctttaaa agctgaacta aacggtaggc aaaattatct ttacggatag gtacttgatt
      721 ctttaccgga cagaacttca ttagcttcgc aggttctact tcttcagatt ccagtaattg
      781 gttgatttct tgtaaagcaa tcacttcata atcttctaac aggatcttat ctgctaattc
      841 ttgcaatttt ttctctggtt ctggctcaag ccagctatgc gtgttgagtg tcatgaattt
      901 cattggtttt ctccttgtct tcatttcgaa acaaaagggt tgtgacaaaa tttttgtcac
      961 aacctctcca gcaaatagtg ttctcctgct aactcctcag taataagctc aatggaccag
     1021 gagctatttt ttgtttggct gtttttatca cttggtgcag ggcagctttt tcacgacctc
     1081 tccactaaac agtattcaaa ctgctgcttc tcggtaataa gcccaaccaa acgaaaaata
     1141 ggaggagcta tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac
     1201 ctctccttac attttaatgg agccgatcac tttaccagct gtcactgtac ctaattgctt
     1261 gatcactact tcttctactt tatcgtttgt aaatacgatc atgatcgttg gatctttttc
     1321 ttcgttttgg atcacatcaa gatccatctc agctaatttc gttcctgttc ctacttcttg
     1381 gccttctttg acagcaatct caaaagctgg ctgattcatt tggactgtat cgattcccat
     1441 atggatcaaa acttcgatac catctttcgt ttccagccca attgcatgtt tgctagggaa
     1501 aatacttgtc acttttccag cgatcggcgc aacaacttct cttgatgttg gaataacagc
     1561 aaagccgtca cccatcattt tttgtgagaa taccggatct tttacttcag ataaaggaat
     1621 cacttctcca gaagctgcgg aaactagttc ttgttctata cctaagtatt gcttactacc
     1681 tgcttgtcca tttcctgtag aaaccatttc tggctctgga atctctgctc ctgattgcag
     1741 cagatcttcg atatctgatt tcaagacatc tgcttttggt ccataaactg cttggactcc
     1801 attgtctttg acgatcaatc ccatcgcccc tgctcgtttc caggcttctt cagtccctac
     1861 tttttctcga tcatttacac ttacgcgtaa ccgagtcata caagcatcga catctttgat
     1921 attttctttt cctcccagca aatgaatgat ctggaccact tgctgatctg ctgttcctgt
     1981 tccgctgtta gatacaacgg tttcttcact gtcattgtcg taatttccgt tacgtcccgg
     2041 tgtagcaaag ttgaattttt tgatcaagaa atttgctaag aaatacgtaa caacaccaaa
     2101 agcgatcaca cacaacacga agttgataag atctccacct agacccgctt tgatagctaa
     2161 cggtgtccga gtcagcaatt cgatattccc aaatgaatgg acccgcaatg gcaagatatc
     2221 tgccatcgca aatgcagccc cttggatcac tgaataaatg acatataatg gcacagcagc
     2281 aaacataaac atgaactcca atggttctgt tacacctgtt agaaagacag ccaacgctgc
     2341 tgaaaaatac attgatttat atcttgcttt cttatcagga tcgacattac gatacatcgc
     2401 aaaagccatt cccatcaaga tacctgaaga accaatcatt tgtcctactt tgaaacgagc
     2461 cggcgtccag ttatgcaaca caaactcata ttgcgacata tcccctgcac ctttaagatt
     2521 cactaaatca gtggcccaag ccaaccataa cggatcttgc ccaaatactt gtgtccccgc
     2581 ctgtgcgcct gataaaatct catacgatcc gccaagctgt gtatagttga taggaatcgt
     2641 cagcatatga tgcaatccaa atggcaatag taaacgttct aatgttccaa ataaaaacgg
     2701 tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat aaaccaaaat tattgattcc
     2761 agcctgtacg tttggccaca ccacagcaag aatcaatgcc acgattgttg accataagat
     2821 aacaacaaac ggaacaaagc gctttccatt gaaaaatgac aaagcatccg gcaattttct
     2881 ataattatag tacttattat aagccattgc cccgacaaat ccagcgatga tccctacgaa
     2941 tactcccata ttcaaagctg gtgcttccaa tacacttgta aaaaatcctt taaccatgat
     3001 cttcgtaccg aacagagtgt gggtaaatgc atcttcattc gcaagcatgt cacttgtgac
     3061 accaaagata gccccagtga tccgattgat taaaataaat gagatcccag ctgcaaatgc
     3121 tccgcctgct ctttcttttg cccaacttcc tcctattgct aaagcaaata aaatatgaag
     3181 attaccaata attgcccaac caatattttc aattactccg cccgttgtga caagcggcgt
     3241 ccaatcagga ttgatcaagg gaattgattt ccctatactg atcatcaacc cagctgccgg
     3301 cataactgct accacaacca ttaaagcctt tccaaatttt tgccaaaact caaaactaaa
     3361 tatctttttc attccgttta gctcctttac gcaatcggtt tcgttaaacg tattatagcg
     3421 tgcgttttct tttttgtaaa tgctttattt tttatttttc tgtctatacc attctataaa
     3481 acctataata cgtgtgttcc cttctataat tttctcctaa aaatttattt ttttaagctt
     3541 ttttctatga aatattttct tttgtctatt gacttgtgca atcggttgcg tttattatga
     3601 acgtataaaa cagctcatta ggaggagaaa aatgaaacag atcaaacgac tatttgctat
     3661 tgatccatgg aaaatcagca cgacacaatt agataaaaaa aatcttcgac tgcaagaatc
     3721 tttaacaagt attggaaacg gttatatggg gatgcgcggg aactttgaag aaacttactc
     3781 aggggaccac catcaaggta cttatttggc gggtgtctgg tatcccgata agacacgtgt
     3841 cggctggtgg aaaaatggtt atccagaata ctttggaaaa gtgatcaacg ccttgaactt
     3901 tatcgcgata gatatttcga tcaacgatca gctgatcgac ttggcttctt tggaaccaga
     3961 agacttttac tgggaattag atatgaaaaa tggtgtatta tctcgtacat tcacaatcac
     4021 tacttctact aataaagtac gcatttcctt cgaacgtttt ctgagcattg tcaaaaaaga
     4081 atcagcttat atctatttaa ctatagaaat gcttgaaggt agcggaacag tcaaactcgt
     4141 atcaaaattg gatggaaatg ttcaaaacga agacagcaac tatgatgaac atttttggaa
     4201 tgagattgat cgtggaacta tagaagaaat cggctatttg acgactaaaa ctattccaaa
     4261 caactttggg attgaacaat ttactgttac tgcagcaatg cgccatttcg ttaatggaaa
     4321 atcagtcatc cctgactatc acgaagatac tttagcatta catggttgct tgtctgcaga
     4381 attatctcaa ggtgagacat ggtcagtgga aaaagaaatt atcgttctta ctagtagaga
     4441 tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa ttaacgaacc agacaaacaa
     4501 ttatatccaa gcaaaagaag agcaaacagc ggcttgggca gaacgatgga agcttgccga
     4561 tgtggtcatc gaaggagacg aagaagcaca acaaggtatt cgattcaatt tgttccaact
     4621 attctctact tactacggcg aagatgaacg attgaatatc ggccccaaag gattcacagg
     4681 agaaaaatac ggcggagcca cttactggga tacagaagcg tatgctgtcc ctctttatct
     4741 tgctttagcc aaacctgaag taactaagaa tctgctgaaa tatcggcata atcaactgcc
     4801 gcaagcaatc cacaacgctc agcaacaagg attaaaaggc gcgctttatc ctatggtcac
     4861 cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca ttcgaggaga ttcaccgaaa
     4921 tggcgcgatt gcctatgcaa tctacaatta tacgaattac accggcgata cggaatattt
     4981 gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc ttctgggcag accgtgtcca
     5041 tttttcaaaa cgagcaaata aatacatgat ccacggtgtg accggcccaa acgaatatga
     5101 gaacaatatc aataacaatt ggtatacaaa tacgatcgca gcttgggtct tgcgttacac
     5161 gcgagaaaat tatttaactt atcaaaaaga agctgctatc actatcagcg aagaagaact
     5221 agccaaatgg aaagatatcg cagagaacat gtacttccca gttgacgaag aattaggaat
     5281 ctttgtccag catgacacat tcttagataa agatctcatg cctgtatccg atattcccac
     5341 aagcgatctt cctctgaatc agcattggtc ttgggacaaa atccttagat cttgcttcat
     5401 caagcaggca gatgtactac aggggattta tttcttcaat gatactttta caaaagaaga
     5461 aaagcaaaaa aactttgatt tctatgaacc aatgactgtc catgagtctt ccctttcgcc
     5521 aagtatccat tcgattttag cagcagaatt aggaatggaa agcaaagctg tagaaatgta
     5581 ccagcgaaca gctcgcttgg atttggataa ttacaataac gacacagaag acggcttgca
     5641 catcacttcc atgactggta gctggttgtc gattgtccaa ggatttgctc aaatgaaaac
     5701 tgcccatgat cagttgagat tcgcaccatt tctgccaaaa acttggaaag cttattcttt
     5761 ccatatcaat tatcgcggac gtttattgtt catctcagtc acagatgaac aagttacttt
     5821 cgagttatta gaaggttctc ctctagaaat ttacgtctat gaacagtctt attctttagc
     5881 ggatcgtcta gaaataacgc taagaaagga ggaagcaaat gtttaaaggg gtattgtttg
     5941 atttagacgg tgtcattacc gatactgctg aattccatta tcgtgcttgg cgaaagcttg
     6001 gtcaggaaat cggcattacg atcgaccaca cattcaacga acagctaaaa ggcgtcagcc
     6061 gagaagactc actctctttg cttttagctt atggcggaaa agaacactct ttttcaaagg
     6121 aagaatttgc agaattagcg aaaagaaaaa acgattatta tttagagatg atccagacga
     6181 tcgaaccaaa agatgtcttc cccggggtcg tgcctttact ggattcttta aaagaagaaa
     6241 aaatcaagat tgctcttgct tctgcaagta aaaacgggcc attcctactt gaaaaaatgg
     6301 gtcttacacc ttactttgat gctattgccg acccggcaga agcaacaaac ggaaaacctg
     6361 cgccagatat cttcctcctt gcagcaaaag ctgtcggact aaaagcagaa gaatgtatcg
     6421 ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc aagtggcgct caaccaattg
     6481 gcgtgggacg tgctgaagac cttggagagg agatcccgct tgtccctgat acgacttttc
     6541 ttacgatcga ttacttgaaa gaaaagtggc atgaccatgg ataatccaga gatctccgtc
     6601 gtaccagttc cagagactga aataaagaaa atcacattga agaacgacta cttagaagtc
     6661 gttcttctca attacggtgc ccgcctgcac caaatcttcg caccagacaa agaaggaaaa
     6721 tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg ttctaacaga caaaagcttt
     6781 tttggagcaa ctgtcggacc tgtagccgga aggatccggg atgcttcttg gggtgagcat
     6841 agattggaga aaaactgcgg atcacaccat atccacggtg gcacgaaagg ctggtctttc
     6901 caattttggg aagtagaagt tttcaaaact ccatcatcaa taggtgtggt cttttactta
     6961 aaagatgaat tttctaccta tccaggacca atcactgcta cgatcactta ccgtttaaca
     7021 aaaaacgaat tagagatgac aacctctgct agtagccttg ttgaaaccat ctgcaatccg
     7081 accaaccatg cctacttcaa cctttcagga aatggaacaa gagacatcta tgaacatcag
     7141 ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag aaaaactgcc tactggaaat
     7201 tggacaaaaa aagaaaaact gccaatcgac tttagaaaat ccccaacact gcaagagatc
     7261 cttgcatgtt atccagatgg actagatgat gtatttcttt tgcatcatcc taggctttcc
     7321 aaaacttcct tacaattatt cgagaagcat tctggtcgcc aaatgactat agcaacttct
     7381 aataaaagta tggtattatt ttctacgact ggattcgaag ctgatttctc tgtcaatgga
     7441 aaacagatgc attcaaacta cggattagca atcgaaccgc aagaaatccc tgatatcgtt
     7501 catttcccca aatttggatc gatcaacctc catcctggtc aagaacggat cagtcagacg
     7561 atttatcggt tctctgcaca ataacaagag aaaggtgttt ttatcatggc tattaccgta
     7621 aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg tctctcgtgt catcaacgat
     7681 catcctagta tttccgagac aacaaagaag aaagtccgaa aaattatgga tgaactaggt
     7741 tatgttccta acatgactgc ccgcaatcta ggaaaacgca tttccagtgc catcggcgta
     7801 atcttacccc ctcttgattc aaaagagcgg atcggtaacc cattttatct tgagatcatg
     7861 gaagcaatca acgaagaagc gcggaattat gatatgacta cagcaatcgc tactgctaaa
     7921 aattttgaat tgctgttaga gaacgtccag cgtatgcatt tacaaaaaca agtagatggt
     7981 tttattctag tgtattcaga tagtcaagat actgtcattg attatttata ccaaaaccac
     8041 atccctttca ctttgatcgg acagccttat catcatgaga acgaaatcgt ttatgtagat
     8101 aatgataacc agcttctagg taaacaagca acagaatttt tgatccagaa tggacataag
     8161 aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt atttcgaacg atatttcggt
     8221 tatcaaaaag caatgatgat ggctggtctt cctgcgttat ccagtgtagt actagagcat
     8281 ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa acgaagccac agcattggtg
     8341 gtcattgacg atatcttcgc ccttcgcacg atgcagttgg cgcaaatgta tggctatgtc
     8401 attccagaga cactctccgt cattagcttc aacaactcga tcttctcaac attaacacac
     8461 ccttacctta caagtatcga tatcgatatc tccgaattag gacgtgtcgc tatgcaaaag
     8521 ctgcgcgaat tgatccatga agaattgagc agtggcgtac gtttggtcat ccctcatcgc
     8581 ttgatcaaaa gagagacagt cgttccttta gaaaacccat actaaatagc tgactcacta
     8641 aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata ctacttctaa
     8701 cacattgttt atctgtaaaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg
     8761 ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc
     8821 acaaaaattt aaaaagcaat tttcttgagt ttcttcttat ttcttggagt taaacgcttc
     8881 tgtcccagcc tcttttttcc ttccttcaat tttttacacg tcgacgattc aataaaaaac
     8941 cgcctaatat tgtaaagaca ggtgcaagaa tcgagaaaaa ggcgaatggc aagtaatgca
     9001 acgcgctaac tttcatcgtt cccataatga atgtgccgct gactccccac ggtacgagag
     9061 aattgacggt cgcacctgaa tccgctagtg ttcgtgtcaa ataggttttt gataagccaa
     9121 cgtcatcaaa agaacgcttg aatgtttctc ctggaagaat gatggacaga tactgttctc
     9181 caacgatcag attgattccg atacagctca aggcagtaaa cccgactaaa cgagaaggtc
     9241 ggtccatctt ttcttttaat tcttgaacga tggtatcaac gatcagatat ttgatcagta
     9301 acccacctaa tgccaatgca agtaaaatca aactagcaga acctaacata ctcatgatcc
     9361 cgcctcgaga aagcagttca tctagctttg gatcaggtgt atttgcgaca taaccattca
     9421 tcaagatgtc tgctgctttt ccgaccgtca aacttttctc atggataaaa gaaagaacga
     9481 gagcagctgt cgatcccgct agtaatgtag ggatagccgg aatttttttg attgcccccc
     9541 caaataaaat aactagtgga atcaatgtcc aaggagagat ccaaaagctc gctttcatgt
     9601 tttggatcat ttcattgata ctagctagat cggctgaatg actgttatgt cctaatatcg
     9661 tataaaaaat caacgaaagg acgaatgctg ggaaatccgt ataacgcata ccagcgatat
     9721 gttcgaatag attaactcct ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg
     9781 agcagttatt ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga
     9841 atccaagtaa atgaccgatc cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg
     9901 aactgccgac agtcactccg acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac
     9961 gcacagaaac aagtttaaat ccataaacca taatcgtagg gatggttcca gataaaatcc
    10021 atgaagcaac taagacacca atcaaaagaa aaatcaggat tggaatgatc cctggagtga
    10081 ttcctttgat gatcccgtca tggatctcat cccatgtgaa ctttttaaat ctgccataaa
    10141 acaacaaaca catgaaagct gcaaggatcg ggatatgtgg cgagagctgg aatccgatga
    10201 tcagtactcc taggagtgcc aacaacacga tcaatacagc gatactttct tttaaagaaa
    10261 tatttttatt catcgtcgat ctcctatttc aaatgaaaag gccctgccat gaaaaa
//
LOCUS       DACDHL010000087        10308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDHL010000087 DACDHL010000000
VERSION     DACDHL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.087"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..318
                     /locus_tag="IV228_002284"
     CDS             169..318
                     /locus_tag="IV228_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8718142.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            329..1843
                     /gene="dltA"
                     /locus_tag="IV228_002285"
     CDS             329..1843
                     /gene="dltA"
                     /locus_tag="IV228_002285"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8718143.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1840..3045
                     /gene="dltB"
                     /locus_tag="IV228_002286"
     CDS             1840..3045
                     /gene="dltB"
                     /locus_tag="IV228_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8718144.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3090..3323
                     /gene="dltC"
                     /locus_tag="IV228_002287"
     CDS             3090..3323
                     /gene="dltC"
                     /locus_tag="IV228_002287"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8718145.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3326..4594
                     /gene="dltD"
                     /locus_tag="IV228_002288"
     CDS             3326..4594
                     /gene="dltD"
                     /locus_tag="IV228_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8718146.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4735..5817
                     /locus_tag="IV228_002289"
     CDS             4735..5817
                     /locus_tag="IV228_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8718147.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5817..6620
                     /locus_tag="IV228_002290"
     CDS             5817..6620
                     /locus_tag="IV228_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8718148.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6690..7430
                     /locus_tag="IV228_002291"
     CDS             6690..7430
                     /locus_tag="IV228_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8718149.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7441..8445
                     /locus_tag="IV228_002292"
     CDS             7441..8445
                     /locus_tag="IV228_002292"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8718150.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8490..10253)
                     /locus_tag="IV228_002293"
     CDS             complement(8490..10253)
                     /locus_tag="IV228_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718151.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3316 a         1703 c         2086 g         3203 t
ORIGIN      
        1 aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc
       61 tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt
      121 gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg
      181 cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt
      241 gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt
      301 atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga
      361 tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt
      421 tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg
      481 cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg
      541 ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat
      601 cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga
      661 agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc
      721 agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac
      781 ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt
      841 tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc
      901 gccattttct tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat
      961 gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc
     1021 agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga
     1081 acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga
     1141 attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa
     1201 tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt
     1261 actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat
     1321 tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc
     1381 aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca
     1441 aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt
     1501 attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg
     1561 ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa
     1621 ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa
     1681 acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga
     1741 ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa
     1801 aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc
     1861 ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta
     1921 tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt
     1981 ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt
     2041 ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca
     2101 ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact
     2161 cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc
     2221 tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca
     2281 tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc
     2341 tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt
     2401 ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat
     2461 tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt
     2521 tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct
     2581 ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat
     2641 ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg
     2701 ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa
     2761 aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta
     2821 atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt
     2881 ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct
     2941 tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc
     3001 ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat
     3061 taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag
     3121 aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt
     3181 tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc
     3241 aagtccctgt atctgaattc gagcgagagg attggtgcac accgaataaa atcattcaac
     3301 aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt
     3361 tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc
     3421 taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga
     3481 tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga
     3541 attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta
     3601 tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg
     3661 ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt
     3721 cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata
     3781 cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt
     3841 attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa
     3901 aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct
     3961 gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga
     4021 tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa
     4081 tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac
     4141 gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg
     4201 tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa
     4261 cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc
     4321 tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt
     4381 caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat
     4441 ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga
     4501 aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca
     4561 acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt
     4621 gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa
     4681 aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat
     4741 acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa
     4801 gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat
     4861 gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt
     4921 tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg
     4981 gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc
     5041 aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta
     5101 acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca
     5161 tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat
     5221 attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt
     5281 atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat
     5341 gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt
     5401 acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc aggagacact
     5461 ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt
     5521 tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag
     5581 gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa
     5641 atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg
     5701 cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca
     5761 gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga
     5821 ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat
     5881 ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact
     5941 tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc
     6001 tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag
     6061 ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt
     6121 ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt
     6181 taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca
     6241 gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt
     6301 tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat
     6361 tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga
     6421 aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt
     6481 ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg
     6541 tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc
     6601 cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg
     6661 taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag
     6721 cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt
     6781 tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg
     6841 atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat
     6901 tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg
     6961 gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac
     7021 atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa
     7081 caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat
     7141 tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg
     7201 atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca
     7261 cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac
     7321 acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca
     7381 aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac
     7441 atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat
     7501 ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca
     7561 atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa
     7621 aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga
     7681 aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa
     7741 gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa
     7801 ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc
     7861 aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc
     7921 aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct
     7981 cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa
     8041 tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg
     8101 tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga
     8161 aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt
     8221 tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa
     8281 atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga
     8341 cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat
     8401 tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg
     8461 gaatccacat atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt
     8521 tgaacagtga tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga
     8581 tccgctacgt ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg
     8641 atgcctcgac taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa
     8701 tgttcctctt gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca
     8761 tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg
     8821 ttgataggaa gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca
     8881 gctaacttct ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac
     8941 agcatttcga aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt
     9001 tggtccgtat gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt
     9061 gtatttactt cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt
     9121 ttgattttta agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt
     9181 agattgtagc tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc
     9241 gttgtttgat agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc
     9301 aagacgatcc aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg
     9361 catgcaagtg aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat
     9421 tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt
     9481 tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag
     9541 cagatgatga aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat
     9601 ggttcaaaaa gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa
     9661 gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag
     9721 atcgtataga aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc
     9781 ggcactgatt gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc
     9841 ggtggcagtc cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg
     9901 atataatttg ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga
     9961 taatctttcc gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt
    10021 cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat
    10081 gtttcgtctt tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca
    10141 ggatcatctt ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc
    10201 gcaggagaaa aaataaaata aaagaaataa gcaattcctg ttaaaaataa caatgataat
    10261 ataatgatta tgctccactt tatacgtaaa taaaaagatt ctttttta
//
LOCUS       DACDHL010000088        10168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDHL010000088 DACDHL010000000
VERSION     DACDHL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.088"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..427)
                     /locus_tag="IV228_002294"
                     /pseudo
     CDS             complement(62..427)
                     /locus_tag="IV228_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(501..1151)
                     /locus_tag="IV228_002295"
                     /pseudo
     CDS             complement(501..1151)
                     /locus_tag="IV228_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            1476..1892
                     /locus_tag="IV228_002296"
     CDS             1476..1892
                     /locus_tag="IV228_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8718152.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1889..2359
                     /locus_tag="IV228_002297"
     CDS             1889..2359
                     /locus_tag="IV228_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8718153.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            2373..3146
                     /locus_tag="IV228_002298"
     CDS             2373..3146
                     /locus_tag="IV228_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8718154.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            3160..3972
                     /locus_tag="IV228_002299"
     CDS             3160..3972
                     /locus_tag="IV228_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8718155.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            4026..4991
                     /locus_tag="IV228_002300"
     CDS             4026..4991
                     /locus_tag="IV228_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8718156.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            5107..7830
                     /locus_tag="IV228_002301"
     CDS             5107..7830
                     /locus_tag="IV228_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8718157.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYIHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7843..8475
                     /locus_tag="IV228_002302"
     CDS             7843..8475
                     /locus_tag="IV228_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8718158.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9374..>10168
                     /locus_tag="IV228_002303"
     CDS             9374..>10168
                     /locus_tag="IV228_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8718159.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3408 a         1577 c         1969 g         3214 t
ORIGIN      
        1 taaaggcgga gccaagagaa ggtgctttat cggtcacaat tgctttcggc tcaccaaact
       61 atttatggag tcgttttaag aaagcatagg ctgcttgcgt atcccgtttc tttcgtaacc
      121 agatatctaa ggttaagccg tccgcttcaa ttgcacgata aagataatgc caacgtccct
      181 taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta tttttcttct
      241 tccaaagata atagaggact ttgctgtact cttgtaccca acgataaatc gtagtatgac
      301 aaacatttat tccacgatca tataacaatt cctgaacttc acgatagctt agattgtaac
      361 gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg cctttaaaat
      421 gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat agattttttg
      481 gaaaactttg caacagaacc gccaatcata tggaagccac cgattaggta ttttatgggt
      541 ttagtcggaa atgtttcaag agcaatttcc atcatatcga taatgccatg atgactacag
      601 ccagtaaaga caatcagacc gtcttcttgc tcaattacga ccaataattc atctgaaaaa
      661 tcatcataaa caaatttatc atccatcttt ttgtataaat tcttggtggt cgagtttgct
      721 ttgcctaggg ggatgatatg gaagctcgga gcaagttctg ttttcgtttt tattgtatgt
      781 aaattctcat gcattttaag gatttcattt ttaggaatac caacatacag aaacgtggtt
      841 aaaatcttta aataatgggg taaagttaca ccttcagctg tataaatagg ggcgtttcct
      901 gattttaaat atcttaaccc gccggtgtgg tcagaatgac cgtgtgaaat aatcactgcc
      961 tccagatcat tcaaagtttc ccccatttta gtcgcattat agactaattt tttattagcg
     1021 ccagcatcga agagatattt cttccctgag tagttaatta agagagataa cccatgtcca
     1081 cttttaaagt tcaaatttat tcttgagttt tcaaataaaa ccttgatttc cactataaca
     1141 tctcctttca catgaacgtg tattcatata ttgtagcaca ttaagttcta ttttttgttg
     1201 taattaccga aaaaaatatt aatggacgct ttttacgctt tatttcgtat gtggaagaaa
     1261 atttcttagg atgtggcggg ggattggggc aaagctaaat tccaattaca gtgatcattt
     1321 aattagacac ctttaaatac ccacactttc atcaacacta taatttaggg aaagaaaagt
     1381 gttggcatga tatagaaaaa cctttaatta acagcgtttc ctaaaattgg cacgcatctt
     1441 gcttatagaa aagcgtaagg ataggaggaa tgaaaatggc aactataatt cttgccagcc
     1501 atggggaatt tgctcaagga ttaaagcaaa cagctacaat gattattgga gatagtgtac
     1561 caattcatgc cttgtcagca tttagagatg aagatgaatc tattttggta caaataaaaa
     1621 aacttttagc cacaatagat atagaagaaa cctacatttt aactgatatt ctaggtggta
     1681 gtgtgaataa tgatatgctc actattatta aagagtatga gggattgcaa ttaattactg
     1741 gaatgaactt acctttagtt atttctattg caacctataa tggaaaaatt ggggaaagtg
     1801 agctagaaaa gcttattaaa gagagtcaac aatcagtgat tgattgtaga aaattattag
     1861 tagaacaact tactggagga gatgacctat gattaagtta gtacgtattg accatcgttt
     1921 gttgcacgga caggtagtat tttcttggag taaatcatta aatatcaatc gtattattat
     1981 tgccaataaa gaggcagcga atgatgaatt caaaaaaatg tctttgaatt tatctaaacc
     2041 gactggcatt aaattaacaa ttttttcagt tgaacagtta ctagaaaaaa tgcctaaaat
     2101 cgaaacactt aacgaaaata tcatgcttat ttttggtaat acaaaagaaa ttcgtcaatt
     2161 ttgtgaagcc tatcctaaag tacaagaaat caactatgga gggataatca aaaaagaagg
     2221 ttccaagcaa tttagtaatg ccattttctt gaatgaatct gagcaagaag atgccaaaaa
     2281 attaaaagct cttggcatta aacaatttat tcaacaggta ccaacttcta aaaaagaaga
     2341 tttgaataca atgttataaa ggaggaatga aaatgctcgt tcagtcaatt ggtttagggt
     2401 taatcggtgt tttttgtatt ttagactcaa ggcttttagg aagaatgaat tttgaacgac
     2461 cgttgattgt cagtacgctt gttggtttgg tacttggaga catggaaaaa ggattaattg
     2521 ttggagcctc tttggaattg atgtccttag gattagtaaa tatcggagct gctgcaccac
     2581 ctgatatgaa tatggcatca attattgcta cagcctttgc cattttatca aatgcgaatg
     2641 cagaagcagc attaacgatt gccattccaa tttcagtgtt aggtcaaata ctaggtatct
     2701 taatgcggac gattttgtca aatttgactc acgttgcgga tcgctttatc gaacaaggga
     2761 aatttagcca agcaaggaga attcatatac tttatggctc gggattatat gctctcatgt
     2821 attttattcc tatttttcta gcaatttatt ttggaacgaa tcttgtttct cagattgttg
     2881 atgcgattcc tgagtggcta acaaatggct taacggttgc aagtaaaatt ttaccagcat
     2941 atgggtttgc gttgttatta caaacgatgt taacgaaaaa aatggtgcca tttttattac
     3001 ttggattttt aattactgct tattctggat tgggaatcat tggtattgca gccttcgcat
     3061 gtattgttgc gtttgtcatg gttcaagttc aaggtaaaaa ctcaaatggt aaccaaaatg
     3121 agccagatcc attagatgac ttataaggag gtagattaag tgaaagagaa agatcaaaat
     3181 gcatggaaat atcggcagtt cttatggcgt tcatgggcaa ttcaagcctc atggaactat
     3241 gaacgacaaa tgaacatggg ttttatgtac ggaattgcac caattctaga taacatttac
     3301 aagaatccag aagatgaagc aatgaagaaa gaagcttacg agcggcatat gatgtactac
     3361 aactgtaccc ctcaaacaag tgcatttgtg ttagggttaa ctgcttctat ggaggagcaa
     3421 tactacgaag accaagaaaa ctttaatccg gaatcaatca gtgcagtgaa gacttctctg
     3481 atgggaccgc tatctggtgt tggtgattct ttctttcaag gaacgattcg ggtattagcc
     3541 tttggactag gaattaattt agcccaacaa ggtagtattt taggtccaat tctagcaatg
     3601 ttaatttcct ttgtaccttc ctttttagtt acttactatg gtgggaaaat tggctataac
     3661 atggggaata aatacttgtc taaactgtat caagaaggtt tgatggataa agtaatgtat
     3721 gtttgctcca ttgtggggtt gatggtcatt ggttcgatga ttgccagcat gatcgggatt
     3781 acaacaccaa ttcaaatggg taaagctttt gtcttacaag atgtattaga tagtattatg
     3841 ccacaatttt taccattagg attaaccttc ttcatgtact ggttattgca gaaaaaggtc
     3901 aaaacaggat ggatgttggc aatctgttta gtcggaggaa ttatttttag tttgttaggt
     3961 atcttacaat aagggaataa ccaataaatt tggttgaata atataaataa ggaggaattc
     4021 aattgatgaa ttcaaaagaa attgttaaag aaatcgtagc gaaaaaagag gtaaaatcaa
     4081 tcgtatttgt tggctgtggc gcttcaaaag cggaccttta tcctgcaaag tattttctag
     4141 accaaaatgc gaagcagtta cggattagtc attacacagc cagcgaattt aactatgcaa
     4201 caccagcatc tgtagatgag tcaactgtcg ttatttcagc ttctttagga ggtacgactc
     4261 cagaaacagt tgaagctaac gcagtagcta aagaaaaagg tgctacagtt attagtctaa
     4321 cacgaagcat tgactcaccg ttaacaaaag atgcagatta tgtaatttat catcgatttg
     4381 cagagaacta tgctgctaaa ttagaaaaaa caggctactg tttagaattg gcagtagaac
     4441 ttttacatca agtagaaggt tatgaccatt atgaagcaat gcaagaagga tttgctacta
     4501 tctatgatct tgcccaaaaa agtgcagaat cagctcgtaa agatgcgaag aaatttgctg
     4561 aacgctttaa agatgaaaaa gttatttatg taatgagtag tggtgctaca catgaagtag
     4621 catactcaac atcaatttgc ttgatgatgg aaatgcaatg gattaattct ggtaccttcc
     4681 attcaggaga atttttccac ggaccattcg aaattgtgga taaagacgta ccgtttatat
     4741 tattaatgaa tgatggaaaa actagaaata tagatgctcg agcattaact ttccttgaac
     4801 gctttgatac gaaaacagaa gttgtagatg ccttggatta tggcttgtct tcacatattg
     4861 ctaaagaagt cttagactac tttaacccat tcgtgattac tgcagtattt agagtttatg
     4921 cagaagaatt gtcagaagca agagaacatc cattaactaa gagacgttat atgtggaaac
     4981 taacttatta aaaaatagat caaactaaat aaggtggctt gagaaattaa gccaccttgt
     5041 tttcttcaat gtgattttat acatatggta aaataactag tacatattgt gaaagcaggt
     5101 ggcaagatgc agcgattaaa tgactatctt tatcaactaa cattgctttt gaaatctacg
     5161 ccttcagata attattcagc tcgcagtatt tcgcaagctt tacagttgaa tagaagtaca
     5221 atcagtagct acttaaatga aggtgttcgt gaagggcttt tcataaaagt aaaaagttac
     5281 ccggttttat ttttacatag aacagctctt gaagaactac atatcacact gaataactcc
     5341 gaaatagaat caattgaaag cttgttaaca atttctcaaa aacccgcatt agatcaagta
     5401 attggctcta aaggaagttt gaaagaagca attgatcaaa ttaaaacggc agtcttatat
     5461 cctggaaaag ggttgccttt attattgatc ggcgctagtg gatcagggaa aacctttcta
     5521 gcgaataaaa tatatgaata tgcagtagaa gaaaaggtca ttcacacatc ggcacctttc
     5581 atagattata attgtgcaca atatgtgagc aatccagagt tactatcctc tgctttattt
     5641 ggctacacga aaggagcatt taccggagca agccaggagc atactggttt gctagaaaaa
     5701 gccgatggag gagtattgtt tttagatgaa gtgcatcgct tatctgaaga agggcaagag
     5761 aaattattta catttatgga tacgggtgaa ttctctccta tgggggataa tagtattcgg
     5821 aaaaaagccg atgttcgatt agtctttgca acaacagaaa atatatatac gactttctta
     5881 ccaacttttc ttaggagatt gccagttatt gttaatctac ctagatttca acaacgtccg
     5941 tcgtttgaac gtctgtcatt gattgatgaa ttttttgttt ctgagagtaa aattttagct
     6001 aaggaactat ctgtttctga tgcattgatt catttcttaa tgaatagtga ttttgaaggt
     6061 aacgtgggga atattaaaaa tattatcaag tatgcttgcg gaaacgcata tatccaccag
     6121 aagagtcagc aaacaattca agttaaatta ttggatatgc ctttagagta cagttataaa
     6181 ttcaaggagc aactaaataa acccagaaag aaaagagcag atcgaatcta ctatccagaa
     6241 gaaacacgac aagtgcaact agaaagaaac aatcagcttt ttgttcaatt ttttgaccaa
     6301 ttacttactg gattctctga agtgattgaa aataacaagg atattaaact atttttagaa
     6361 gaaatggtta ccaaggtaac acaagtaatg gatacgctta tttttcaaga ggattacgaa
     6421 aaagagcagt ctttatacgc cattctaaac tatcatattc gtcaaagtat tgattttatg
     6481 cgagaaagct acgggtttga acaagatggt aaccgggtag tcgctttggc caactatcta
     6541 tatgtcaagg ataatcaagt gatttttgaa aatagtcttc aagaaaagaa aaccaaagat
     6601 cagcttaacg aattttttgc taatgttctt gaaacccctt attggtatgc aaaaaaaatc
     6661 ttaagccttt tatctgagtg tttagatcaa cctgtgtatg aagaggacat tttctttatt
     6721 acgctatatt tttatagttt gaatgtcacg aatgttaaaa caggtattaa gagtattgtg
     6781 ctagctcatg gttattctac agctagtagt atggcgaatg ttgccaaccg tatgctgaat
     6841 aagaacgttt ttcaagctta tgatatgcct ttagatatta cattagagaa aattgaagca
     6901 aaattgattc aattccttgg agattatcga acagattcag gcttgatttt attggtagat
     6961 atgggttcac taaaccaact aggcggttta ttagtgaatc atctaaatgg accactgtta
     7021 atcattgatc atatcaatac accgttatta ttggaagtcg gccaacaagt attaaacaat
     7081 aaaagtatca ccgaaatagg agcagaaatt gaaacaagtc ttactttcaa aaagcagtta
     7141 ctacttccca agaaaaagaa aaagaaagcc atcattactt gttgttatac agggattggt
     7201 agtgcgaccc agattcagga gattttaacg aaatgcttga aagatgcagt tagagaatta
     7261 gtaattattc cgtatgatta caaaaagctg ttagaaaaca agaattttga gactcctttt
     7321 caattatacg atgtcatcat gattgtaggt acagaagatc ccaaaattaa tcagattcca
     7381 tatattggat tagatcgcct gattaatggg gaagcaattg gcgaatttgt tcattacttg
     7441 gaaaaagagc tgactattga agtgaacgat ttacaaaaag aattaatttt caatttttca
     7501 attagtaaaa ttgtcgaaaa tttaaccatt ttggatccag ataaggtgct atcttttgta
     7561 caaaaagcaa ttaaagaatt atcgaagtta ttaaattttg atttttctaa taatcagctg
     7621 tttttgttgt acattcactg tagttgtatg attgaacgaa ttgtacgtaa agaaagtgtg
     7681 gatgagcaag aggacattga agaatatatg caagcaaatg gagaaataat ggataaaatt
     7741 catagcgctt tttttgagat tgaaagggaa tacacaattg aaataccaga tttagagttg
     7801 cgattattga gcgatattgt tcaaggatag gagtgaattc gaatgacaat gggagtaatt
     7861 tttgatatgg atggggtact catagacagc gagacatttt attttgagag aagaatgaac
     7921 ttctttcaag aactggagat agatccagga tcaaaccaga ttgatgatta tgtggggaaa
     7981 acagaagctg caatatggga aacattagtt cctgaggaca aatcattaag agaggaactt
     8041 tatccgaaat atgtagatta tcgaaaaacg catccaattg atttcgctaa agatttacga
     8101 aaagaagtga aaccgttact tgacttcctc aagaagcggg atataccatt ggcactggca
     8161 tcttcttctc cagttgttga gatagagagt atgctcattc aatgtgaact tactgaatat
     8221 ttttcctttg ttataagtgg tgagtcatta aaacaaagca aaccacatcc cgaaatatat
     8281 ttaaaagcga aacagcaatt agactgtgac ttctttattg cagtagagga ttcaacgatt
     8341 ggtattaaat cagcaaaggc agcaaatatc tatacggtgg cactaaaaca accaatgcaa
     8401 atcgatcaga gtgaagcaga catacaaatt gaaagtctac aagaactgga acaaattgtt
     8461 ttagaaaaag gttagtgaat taatggaaat aaggctatat atctgtgcta gctattttga
     8521 ctaaccaaaa aaacttgcta taattcaggt gtagtaaaag gtgctggtaa caccttttac
     8581 gaaaatgctt cggcagtgat aatgggattg gctttttaag ttttttattt tgttcgcaat
     8641 ccctaaggtc atgtgaatga tcctagggat tgctagaata ttttgcaaca ttctatctat
     8701 ttcagaacgt aaccttcccc agttcattgg gattcattag catacaacca acatttcaaa
     8761 aagaaattgc actacaccat tattagctat tttgactaac cccaaaaaac ttgctataat
     8821 tcaggtgtag gtaaaaggtg ctggtatcac cttttacgaa actgcttcgg cagtggtaat
     8881 gagtgtggct taaaggacta gtcctttaag ctttttactt tgttcgcaat ctctaagatc
     8941 aagttaatga tcgataagat tgctagaata ttttgcaaca ttctatttca gaacgtaacc
     9001 tttcccattc cactgtgatt cattggcaac cctcctcaca accaacgttt caaaagaaat
     9061 tgtaaagttg ttggggtacc acgaccgaac aatactacac ctgaatgata gtaagttgtc
     9121 tcattaagta tagcatatcc tctttttggg taaatagaaa tcggttacat tttttatcca
     9181 agaccaagtt ttttcatttg gtcttatttg ctatacccaa acaattccgc agtatagtcg
     9241 acggtattga ttttatcagg tgagtaatag aaacttgttt tccttcccca aaacattacc
     9301 tataaaggag aaaaaatggt atcgtaatta tataggtata ggatgaaatt tttttctaag
     9361 aggagggttg ttgatgaaaa atttacagtt aaaatttatt gttgatccat cgattgtacg
     9421 ctggtttcgg attatcaatc aatttgagcg caatcaactt gccacaaaag gagaattagc
     9481 aaagagtaat catgtatctg aacgaaccat tcaaacagat gtcaataaaa tgaaggacta
     9541 cttttctgaa agtgttcaat ttgtttcaac gaaaagtggg tattcttttc agaaggaaag
     9601 accggttctt tttctcaagc agaaggagca actactggag aacgaaattt tatttgagtt
     9661 gttagggaat gtcttttatg gcgaattgga agatatgacg gagctaattg atcgttttca
     9721 tttatctgaa gcgacttttc gtcggtattt gaagcagatt caaccagttt tggaagaata
     9781 cggcttagaa ttgagtcttt atcctcttga tttaaaagga gaagaggcaa atattcgcaa
     9841 gttttttaaa gacttctatt atgaagggga gatgacgccc catactcttg ttccaccaag
     9901 ggatcttcat gaacttgtgc aagctacatt ctatgataaa tttgagtatt tctcgattgg
     9961 aacagggact tctcctgctg aattttatta tagtttgtat attgcgattg agcgtgtgag
    10021 acaaggaaaa acgatagcca ttcctcaagg tctgattgag cgagtaattg ctagtgagaa
    10081 ttttcgctta atgtattcct tgaaagaagc gattcaaaca aattatcaag ttgctttatc
    10141 agaagaggag ttttgttggt tgtattta
//
LOCUS       DACDHL010000089        10125 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDHL010000089 DACDHL010000000
VERSION     DACDHL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.089"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(513..1361)
                     /locus_tag="IV228_002304"
     CDS             complement(513..1361)
                     /locus_tag="IV228_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8718160.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1525..2427
                     /gene="gnd"
                     /locus_tag="IV228_002305"
     CDS             1525..2427
                     /gene="gnd"
                     /locus_tag="IV228_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8718161.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2429..3430
                     /locus_tag="IV228_002306"
     CDS             2429..3430
                     /locus_tag="IV228_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8718162.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3427..3711
                     /locus_tag="IV228_002307"
     CDS             3427..3711
                     /locus_tag="IV228_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8718163.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3744..4541
                     /locus_tag="IV228_002308"
     CDS             3744..4541
                     /locus_tag="IV228_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8718164.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4555..5388
                     /locus_tag="IV228_002309"
     CDS             4555..5388
                     /locus_tag="IV228_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8718165.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5406..6263
                     /locus_tag="IV228_002310"
     CDS             5406..6263
                     /locus_tag="IV228_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8718166.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6285..6794
                     /locus_tag="IV228_002311"
     CDS             6285..6794
                     /locus_tag="IV228_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8718167.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6831..7232
                     /locus_tag="IV228_002312"
     CDS             6831..7232
                     /locus_tag="IV228_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718168.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7344..7697
                     /locus_tag="IV228_002313"
     CDS             7344..7697
                     /locus_tag="IV228_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718169.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7731..8174
                     /locus_tag="IV228_002314"
     CDS             7731..8174
                     /locus_tag="IV228_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8718170.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8191..9276
                     /gene="uxuA"
                     /locus_tag="IV228_002315"
     CDS             8191..9276
                     /gene="uxuA"
                     /locus_tag="IV228_002315"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8718171.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9269..9982
                     /locus_tag="IV228_002316"
     CDS             9269..9982
                     /locus_tag="IV228_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8718172.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEILLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3402 a         1599 c         2204 g         2920 t
ORIGIN      
        1 tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca ataatagaaa
       61 atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc caaaaattcg
      121 atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa taaaattaca
      181 aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa
      241 aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag aaaagataaa
      301 ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg aataatacca
      361 atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta ttgatgttct
      421 gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg ctttttttgt
      481 tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga aatagtatgt
      541 cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa aatcacatat
      601 agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga tcggaattct
      661 gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga agaattagct
      721 tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc aatatggatt
      781 gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc cgtcaataaa
      841 gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat aggggaacgc
      901 aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc gaaaaaatag
      961 acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg ttgtaacaac
     1021 ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc catagtgagc
     1081 tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc attttcttgg
     1141 atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt tgtaaattga
     1201 aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg actatgactt
     1261 actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg gctgaagtcg
     1321 ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc tcattttcat
     1381 aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag agtaggaaaa
     1441 aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag
     1501 ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga
     1561 ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca
     1621 agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa
     1681 tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata
     1741 accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa
     1801 ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa
     1861 atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc
     1921 ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta
     1981 gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg
     2041 atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta
     2101 caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg
     2161 gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta
     2221 agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg
     2281 ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca
     2341 caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga
     2401 catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc
     2461 cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt
     2521 tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg
     2581 attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa
     2641 agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc
     2701 agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt
     2761 agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc
     2821 aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa
     2881 caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg
     2941 taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga
     3001 aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg
     3061 tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca
     3121 gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc
     3181 gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga
     3241 agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca
     3301 tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt
     3361 cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat
     3421 aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat
     3481 ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa
     3541 agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt
     3601 ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct
     3661 gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg
     3721 gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg
     3781 gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata
     3841 aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta
     3901 taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag
     3961 atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg
     4021 aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc
     4081 attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc
     4141 tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa
     4201 gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc
     4261 gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc
     4321 gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc
     4381 tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg gctgaattta
     4441 ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac
     4501 agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa
     4561 acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc
     4621 tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc
     4681 gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg
     4741 atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca
     4801 gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt
     4861 gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact
     4921 tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga
     4981 tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt
     5041 gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc
     5101 gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact
     5161 gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt
     5221 gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc
     5281 gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac
     5341 aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat
     5401 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
     5461 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
     5521 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
     5581 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
     5641 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
     5701 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
     5761 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
     5821 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
     5881 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
     5941 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
     6001 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
     6061 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
     6121 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
     6181 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
     6241 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
     6301 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
     6361 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     6421 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     6481 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     6541 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     6601 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     6661 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     6721 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     6781 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     6841 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     6901 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     6961 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     7021 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     7081 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     7141 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     7201 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     7261 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     7321 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     7381 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     7441 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     7501 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     7561 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     7621 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     7681 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     7741 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     7801 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     7861 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     7921 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     7981 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     8041 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     8101 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     8161 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     8221 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cggggataaa tgggataatt
     8281 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     8341 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     8401 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     8461 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     8521 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     8581 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     8641 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     8701 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     8761 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     8821 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     8881 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     8941 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     9001 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     9061 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     9121 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     9181 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     9241 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     9301 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     9361 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     9421 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     9481 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     9541 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     9601 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     9661 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     9721 gttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     9781 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     9841 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     9901 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     9961 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
    10021 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
    10081 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgt
//
LOCUS       DACDHL010000090         9874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDHL010000090 DACDHL010000000
VERSION     DACDHL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.091"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(22..267)
                     /locus_tag="IV228_002317"
     CDS             complement(22..267)
                     /locus_tag="IV228_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718173.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(382..960)
                     /locus_tag="IV228_002318"
     CDS             complement(382..960)
                     /locus_tag="IV228_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8718174.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1037..1567)
                     /locus_tag="IV228_002319"
     CDS             complement(1037..1567)
                     /locus_tag="IV228_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8718175.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1571..2431)
                     /gene="rlmB"
                     /locus_tag="IV228_002320"
     CDS             complement(1571..2431)
                     /gene="rlmB"
                     /locus_tag="IV228_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8718176.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2424..2819)
                     /locus_tag="IV228_002321"
     CDS             complement(2424..2819)
                     /locus_tag="IV228_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8718177.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2816..4228)
                     /locus_tag="IV228_002322"
     CDS             complement(2816..4228)
                     /locus_tag="IV228_002322"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8718178.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4225..4761)
                     /gene="cysE"
                     /locus_tag="IV228_002323"
     CDS             complement(4225..4761)
                     /gene="cysE"
                     /locus_tag="IV228_002323"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8718179.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5083..6543)
                     /locus_tag="IV228_002324"
     CDS             complement(5083..6543)
                     /locus_tag="IV228_002324"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8718180.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6713..7870)
                     /locus_tag="IV228_002325"
     CDS             complement(6713..7870)
                     /locus_tag="IV228_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8718181.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7904..9274)
                     /gene="radA"
                     /locus_tag="IV228_002326"
     CDS             complement(7904..9274)
                     /gene="radA"
                     /locus_tag="IV228_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8718182.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9337..9864)
                     /locus_tag="IV228_002327"
     CDS             complement(9337..9864)
                     /locus_tag="IV228_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8718183.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2509 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaaagaaat gcaataaaaa taaataagaa
      361 gctcattaag atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt
      421 gagttttact ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc
      481 catgtctaca aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga
      541 aaactggatt tcgcaatttt cgattgcttc ttttaataaa agatgctgaa tcgctaaagg
      601 ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat
      661 tgagacttca tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata
      721 gatttgattg cttataatgc ttttataata acttccaaag ctaacacctt tgcttttttc
      781 gtaagttctc aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac
      841 ataatttctt aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata
      901 cagggcagca aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat
      961 aaaataattc ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac
     1021 tgattagaac gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt
     1081 tttttaactg ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt
     1141 tattttcttg ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag
     1201 aagttcgtaa tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca
     1261 ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag
     1321 ccgtttcgtc ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga
     1381 tgcccggaac tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat
     1441 aatttgataa acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa
     1501 gctggacaag ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt
     1561 ttttcatggt ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag
     1621 caacactggc atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca
     1681 tttctttttt caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg
     1741 atcctttggc attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa
     1801 atcctgtttc tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga
     1861 catgttcgac tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt
     1921 gttttggtat gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa
     1981 aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt
     2041 ttgttcgttc aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga
     2101 ctccttgatg cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga
     2161 ctggcacagc ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat
     2221 cttcttgaag aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt
     2281 gcccaaagac gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc
     2341 gtccagtttg ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg
     2401 attgatgttt gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag
     2461 cggatcaatt cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct
     2521 tcgaaccctg ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt
     2581 ttagcatttc ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc
     2641 agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga
     2701 tgcagcttat tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg
     2761 taaatcgcat ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg
     2821 cttctcttct ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca
     2881 acaaatcacg aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt
     2941 cttcgatcaa ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga
     3001 agatcgaaag ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt
     3061 tcggctggtc tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat
     3121 ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca
     3181 aagcgatttt atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca
     3241 aattgtcaaa cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt
     3301 cactgtagcg gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg
     3361 gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt
     3421 tttcatcgtc ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt
     3481 tccctgtttt cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt
     3541 gcccgccacc atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt
     3601 caatatgcca accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt
     3661 ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg
     3721 tacgctgact tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat
     3781 tttcaaattt acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt
     3841 caaccaacac ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca
     3901 gtgtagcagg ctcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat
     3961 cagcaacttc tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta
     4021 catccgtgaa attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa
     4081 ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg
     4141 gtccgcagac atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc
     4201 tggtcaatgt attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg
     4261 aacgactttt gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac
     4321 ggcacctgca ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt
     4381 cgcagagagc atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt
     4441 gccgcctaat gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc
     4501 aatgacgatt cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg
     4561 gatctcaaca ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag
     4621 ccggtgctga tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta
     4681 tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat
     4741 cgctcgggta aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat
     4801 catcttctta taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt
     4861 ccgcgtggtt ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat
     4921 acgttcttgg ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa
     4981 gaccactccc agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc
     5041 tcttgtaatg atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt
     5101 taaatgtgct aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc
     5161 atgcatttgt cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc
     5221 agtttctttt tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat
     5281 ttgttctagc tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac
     5341 ttcttttgct gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac
     5401 gatttctgca gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc
     5461 gattgtcttt tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca
     5521 catgtcagta aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag
     5581 ttttttcgca tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat
     5641 caattcttgg tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat
     5701 aaagttgaac attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa
     5761 gattgattcg tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat
     5821 atgtccgaag gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt
     5881 attggcaata tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac
     5941 cgcaacagca aaattataag ttggcatacc atcgcgtttt tgtatgacaa agtcgccacc
     6001 gatattatcg gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt
     6061 attgcgaggc acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg
     6121 ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg
     6181 ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt
     6241 gctagcaagc agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta
     6301 agggccgtat tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag
     6361 gttttccaac tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat
     6421 acgaataata aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct
     6481 tgcatttcca atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt
     6541 cattttcttc ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac
     6601 ggcttctcaa atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta
     6661 aatagtaaag ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg
     6721 attgtttagt ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag
     6781 gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga
     6841 tacgttcatt catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt
     6901 aggctactcc ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac
     6961 cagggatgac gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat
     7021 tttggaattc agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa
     7081 gcttagccaa tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt
     7141 acatttcgac agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac
     7201 gaccacgcac acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa
     7261 caaaattagg gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc
     7321 gaccgtcaat gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt
     7381 ttcgttctaa cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc
     7441 gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat
     7501 agcctaagat gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat
     7561 tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac
     7621 cgaagagaag ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag
     7681 ctgagatatg tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc
     7741 tgttggtaaa tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg
     7801 agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac
     7861 gtttttgcat cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct
     7921 tcaacgtttc agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt
     7981 tacctaagtt gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga
     8041 ctcgttgttc aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga
     8101 aacattccgt tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag
     8161 ctaaatcaat cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt
     8221 gattctgaag aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat
     8281 tgaaatcaag acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg
     8341 cttggatctc taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag
     8401 ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc
     8461 catgctcatg catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg
     8521 ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca
     8581 tatgttctaa cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa
     8641 aaatggctat cccatttgtt ttagcgattt tgagtagttc tgcagtggtt tctcttactt
     8701 ggctgacact tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa
     8761 tgataacata atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt
     8821 cagcgtatag ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct
     8881 gctctgcgct ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct
     8941 gagaaacttg taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca
     9001 tagaccctgg caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga
     9061 tccgaggttc ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg
     9121 tcaaacttgt ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat
     9181 tccattttcc gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt
     9241 ggcaaacaaa ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca
     9301 tgagacatta aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc
     9361 gccttgacgt tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc
     9421 ttggccgata cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg
     9481 gaacatgata tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac
     9541 tccgtttgtc aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa
     9601 aaattcatct tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg
     9661 tttttcattt tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt
     9721 ccagcctgct ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc
     9781 agcagcatga tgtgtagcgc gctgcggtag attgatattt tgatctttat atttagaaat
     9841 aacttcaaat cctctttttc tcaccggttt tcct
//
LOCUS       DACDHL010000091         9855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDHL010000091 DACDHL010000000
VERSION     DACDHL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.092"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            407..490
                     /locus_tag="IV228_002328"
     tRNA            407..490
                     /locus_tag="IV228_002328"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:441..443,aa:Leu,seq:cag)
     gene            complement(814..1452)
                     /locus_tag="IV228_002329"
     CDS             complement(814..1452)
                     /locus_tag="IV228_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8718184.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            1787..2221
                     /locus_tag="IV228_002330"
     CDS             1787..2221
                     /locus_tag="IV228_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8718185.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            2244..2564
                     /locus_tag="IV228_002331"
     CDS             2244..2564
                     /locus_tag="IV228_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718186.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            2705..2896
                     /locus_tag="IV228_002332"
     CDS             2705..2896
                     /locus_tag="IV228_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718187.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(2967..3224)
                     /locus_tag="IV228_002333"
     CDS             complement(2967..3224)
                     /locus_tag="IV228_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8718188.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            3382..3645
                     /locus_tag="IV228_002334"
     CDS             3382..3645
                     /locus_tag="IV228_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718189.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            3669..3857
                     /locus_tag="IV228_002335"
     CDS             3669..3857
                     /locus_tag="IV228_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718190.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(3940..4125)
                     /locus_tag="IV228_002336"
     CDS             complement(3940..4125)
                     /locus_tag="IV228_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718191.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            4340..4711
                     /locus_tag="IV228_002337"
     CDS             4340..4711
                     /locus_tag="IV228_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8718192.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(4861..4933)
                     /locus_tag="IV228_002338"
     tRNA            complement(4861..4933)
                     /locus_tag="IV228_002338"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4898..4900),aa:Gln,seq:ctg)
     gene            complement(4979..5407)
                     /locus_tag="IV228_002339"
     CDS             complement(4979..5407)
                     /locus_tag="IV228_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718193.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(5467..6225)
                     /locus_tag="IV228_002340"
     CDS             complement(5467..6225)
                     /locus_tag="IV228_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8718194.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            6520..6717
                     /locus_tag="IV228_002341"
     CDS             6520..6717
                     /locus_tag="IV228_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718195.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            6823..7305
                     /locus_tag="IV228_002342"
     CDS             6823..7305
                     /locus_tag="IV228_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718196.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(7363..7959)
                     /locus_tag="IV228_002343"
     CDS             complement(7363..7959)
                     /locus_tag="IV228_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8718197.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            8346..9812
                     /locus_tag="IV228_002344"
     CDS             8346..9812
                     /locus_tag="IV228_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8718198.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT         3245 a         1562 c         1963 g         3085 t
ORIGIN      
        1 aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt atgatctcta
       61 gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag taatcaacag
      121 aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag aggaaaaaag
      181 aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa cattgaaatc
      241 gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga gttgctcgga
      301 tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag aacaaaaata
      361 tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg ctatggcgga
      421 attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag ttcgagtctt
      481 cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga tgagaaagaa
      541 aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc agattcaaat
      601 aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat tgattcaaat
      661 agtagattta tagattctga tacaagaaat actggcaaac gatagttaag gaataaagac
      721 taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct cttactatat
      781 tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg cttcccagaa
      841 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
      901 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacggccgat
      961 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
     1021 tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
     1081 ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa caggttgttc
     1141 agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
     1201 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagtaga tcaggttaat
     1261 atcagaaatt gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt
     1321 ttgagaaatc gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc
     1381 agtcgttccc atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt
     1441 aagtgatgtc atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg
     1501 aggaatactt taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca
     1561 aatccacgtt ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc
     1621 acataatgca gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa
     1681 taacttgaaa gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt
     1741 atttttgctt gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata
     1801 ccagaacatc atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc
     1861 ccttgactta gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt
     1921 tgagctgacc catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac
     1981 agaagtagcc atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca
     2041 cgcaatagtt gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga
     2101 aaatattgat ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt
     2161 tggatcgacc gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta
     2221 atgagataag ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa
     2281 aaatagaaat tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg
     2341 ctttagtaaa gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac
     2401 aagataaagg attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg
     2461 ataaatttat ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat
     2521 tagataaatt aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc
     2581 agcctttaga aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa
     2641 actagaataa tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag
     2701 gcaaatggaa gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata
     2761 tgaagaagcg aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat
     2821 cgcacaagaa tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt
     2881 tggtataaca gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc
     2941 gttttctttt taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa
     3001 ataataagaa aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt
     3061 gtttcccaat gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc
     3121 agtaaaactg aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata
     3181 ataaaaaaag gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata
     3241 aataaagtcc atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat
     3301 ttatccggta aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt
     3361 gaaagaagga gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc
     3421 aataatattc gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg
     3481 aataaaataa atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga
     3541 agttattata gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat
     3601 ctagatacca aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga
     3661 ggaaaaaaat gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa
     3721 agattgatgt aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga
     3781 atcgtaaata tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc
     3841 cgacacgttt attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag
     3901 cattgtcctc ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa
     3961 tttgtttttg acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata
     4021 aggtcctaat tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt
     4081 cttaatcatt tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt
     4141 tgctatcaac atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca
     4201 ataatatcaa ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag
     4261 gagaaaaatc tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat
     4321 ataacaagga ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag
     4381 tggtattttg ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg
     4441 tatacttgat cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa
     4501 accagaggaa gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa
     4561 tgggaaagaa cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt
     4621 gaaaattagc aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt
     4681 tccggagaaa gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga
     4741 ttttattatt cataatttaa ttatgtaaac taaattctag tagtaaaaat aatacaataa
     4801 tccccctgca aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa
     4861 ttgggatggc agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt
     4921 tggctacatc ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt
     4981 atttttcttg atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt
     5041 ctaatccagc aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat
     5101 attctttgac aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa
     5161 agcgagtata ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact
     5221 cttgatctgc gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg
     5281 tagcaggtct gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct
     5341 tgcagtcacg gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt
     5401 tttccattat ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt
     5461 gaattactag cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga
     5521 aaaatcgttg atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt
     5581 ttctttgtca tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc
     5641 attatttgtg gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac
     5701 agaagtccag acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc
     5761 ggtttttgga aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg
     5821 gggaacatag acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt
     5881 aggatcgcca tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa
     5941 ctggttttta gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc
     6001 acagccattt tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac
     6061 ttctgctttc tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat
     6121 tgtatgttct ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc
     6181 tgtgccccct agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt
     6241 tcagctgttt atctttccta ttataaatgt atttttattg gaaatggaag tattatattt
     6301 taatatgtag gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct
     6361 tctaaataca acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa
     6421 ggttgatttt taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg
     6481 taatgtggag aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat
     6541 ttaggagaac aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt
     6601 gtatcaaaaa tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa
     6661 gaattattag atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga
     6721 aaacaattgg acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt
     6781 cttttcctat tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg
     6841 gttctattgt ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa
     6901 attatttatc ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca
     6961 ctagtcattt ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc
     7021 ttgtcagcaa tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga
     7081 cttgttttga atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta
     7141 ttatatcagc caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga
     7201 ggaccgatgc tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc
     7261 ttaaacgatg aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga
     7321 caagatgttt gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa
     7381 gcgatcggca tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac
     7441 taattgcgaa cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc
     7501 aataactggg ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca
     7561 agtattagct gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata
     7621 taattcaaat tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt
     7681 accataacca tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg
     7741 agtcaatggg cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa
     7801 aaggataact gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc
     7861 tttgatcaaa gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat
     7921 tgaacggcgg tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt
     7981 tgcgttttgc atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag
     8041 taaacagata tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat
     8101 cgttatgtaa ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt
     8161 atttgaaaaa aacagctgat atataattat taaatctcta tcttcttttt tttatgatat
     8221 tgccattaaa aattacttgc ttttatagaa aattagttta tatttcttaa ataagttttt
     8281 tagttaaatt tttcggtaaa gatgtataat attttctgaa aatttaaaga attggggtat
     8341 agattatgga aaaacagcaa gacaagggct tcttcgggca gccgaaggga ttgtcaactt
     8401 tgttcttcac tgagatgtgg gaacgattta gttattacgg tatgcgtgca ttacttatct
     8461 attatatgta cgatacagtt gccaacgggg gacttggatt accacgagca acagcattag
     8521 ctatcatgtc gatctacgga tctttgatct acatgtctag tatcgtcggc ggatgggtat
     8581 ctgaccgagt gttaggagca aaacgcactg tattcttcgg tggagttttc attatgcttg
     8641 gccacatcgt cttggcttta ccatttggtg taagtacatt gtttgtttcg atggcattga
     8701 ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg catggtcgga catctttatt
     8761 caaagactga cttgcgaaga gatgcaggat tttcgatttt ttacatgggt gtcaacttag
     8821 gggcattatt agcaccacta atcgttggta caatcggtca agaatataac tatcatttag
     8881 ggttctcttt agctgctatt gggatgttct ttggattatt acaatacatc atccaaggac
     8941 gcaagacatt agacggagtg ggtacaacac cgccgaatcc tttgacaact gaagaacgta
     9001 aaggattcat ccgtaacttg atcatcgcac ttgttgttat cgctgcagta ttcggcgggg
     9061 cacaactaac tggtcatctg acaattgact tttttgtcaa tacaatcagt gtattgggga
     9121 ttatcttacc gttgtattac tttatcaaaa tgctgacttc aaaagatgta actgctgaag
     9181 aaaaaccaag agtatgggct tatatcccat tattccttgc agcaatcatt ttctggtcat
     9241 tacaagaaca aggttcatct atcttagctt tatttgctag tgaacgtaca caatctacgc
     9301 tatttggtgt aacgatccaa gcaagctggt atcaatcatt gaatccaatt ttcatcttta
     9361 ttctgacacc agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa ccatctacag
     9421 tagtgaaatt ctctcttgga cttttatttg caggattatc gtacttgcta ttgatgttgc
     9481 cgggtatgct ttatggtaca agtggaaaag taagtccatt gtggttgttg ggaagtttct
     9541 tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg acaacaaaat
     9601 tagcaccacg agcatttgaa tcacaaacaa tcgctatttg gttcttagct gatgcttctt
     9661 cacaagcaat caatgcacaa attgcacgct tctacacacc gggaacagaa tctgcttact
     9721 tcggtattat cggtgcagta gcaatcgtcg gtggtatctt gctatttact gtgaaagaac
     9781 caatcaaaaa actaatgggc tctattcatt aatcaatgaa cataaaaaca ggaattgctt
     9841 atttgcagca gttcc
//
LOCUS       DACDHL010000092         9767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDHL010000092 DACDHL010000000
VERSION     DACDHL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.093"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            201..680
                     /locus_tag="IV228_002345"
     CDS             201..680
                     /locus_tag="IV228_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718199.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="IV228_002346"
     CDS             713..2941
                     /locus_tag="IV228_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8718200.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="IV228_002347"
     CDS             3070..4713
                     /locus_tag="IV228_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8718201.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="IV228_002348"
     CDS             complement(4786..5331)
                     /locus_tag="IV228_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8718202.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="IV228_002349"
     CDS             complement(5354..5839)
                     /locus_tag="IV228_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8718203.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="IV228_002350"
     CDS             complement(5829..6641)
                     /locus_tag="IV228_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8718204.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="IV228_002351"
     CDS             complement(6780..8312)
                     /locus_tag="IV228_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8718205.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="IV228_002352"
     CDS             complement(8421..9452)
                     /locus_tag="IV228_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8718206.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3248 a         1824 c         1882 g         2813 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctcc
//
LOCUS       DACDHL010000093         9560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDHL010000093 DACDHL010000000
VERSION     DACDHL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.094"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            91..297
                     /locus_tag="IV228_002353"
     CDS             91..297
                     /locus_tag="IV228_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8718207.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(499..1500)
                     /locus_tag="IV228_002354"
     CDS             complement(499..1500)
                     /locus_tag="IV228_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8718208.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1505..3418)
                     /locus_tag="IV228_002355"
     CDS             complement(1505..3418)
                     /locus_tag="IV228_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8718209.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3586..4092)
                     /locus_tag="IV228_002356"
     CDS             complement(3586..4092)
                     /locus_tag="IV228_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8718210.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4252..4692
                     /locus_tag="IV228_002357"
     CDS             4252..4692
                     /locus_tag="IV228_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8718211.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4718..5875)
                     /locus_tag="IV228_002358"
     CDS             complement(4718..5875)
                     /locus_tag="IV228_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718212.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5878..6234)
                     /locus_tag="IV228_002359"
     CDS             complement(5878..6234)
                     /locus_tag="IV228_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718213.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6532..7506)
                     /gene="sgrA"
                     /locus_tag="IV228_002360"
     CDS             complement(6532..7506)
                     /gene="sgrA"
                     /locus_tag="IV228_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8718214.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7702..8541)
                     /locus_tag="IV228_002361"
     CDS             complement(7702..8541)
                     /locus_tag="IV228_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8718215.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8726..>9560
                     /locus_tag="IV228_002362"
     CDS             8726..>9560
                     /locus_tag="IV228_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8718216.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VM"
BASE COUNT         2871 a         1932 c         1438 g         3319 t
ORIGIN      
        1 tgatcatttt attcacatcc taacaaactt gtcacattta tagattctta atctatttat
       61 catacaatta taaaaaagga ggtgctgctt atgaaagcta tccatatttc tttgcttttt
      121 ttcgctagtg cattgatttt atttgctatt catctttgtt cccagcctta tatcaataac
      181 aacggaatat tagtggaacc agcattcttt tgcttaccac tcggcttttt gtcccttctt
      241 gcctccgcag gcttcggatt tgttgctttt tttaaacgat caaaacaaca aatctgactt
      301 ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa atttcgaata
      361 aaaggtgccc cttgccatct ctaactcctc agtcacaagt tacaatattc ataaaccatg
      421 aaaagcttgt ttattgaaat tgctccttgt gacttggagc taagtgacag attacaacct
      481 cttttgcatc ataagacttt actccttgaa aaacattact aacttttgat atgtttcttg
      541 cgcttgttta ttgctgaaat caaactgata ttcatggttc aaaacagcat ttttctccgc
      601 ataatctact tttgtgactt tcacccccgc tttctcaaat acagaagtta cttcttccat
      661 ttgtttcgta aacgtatttg tatttccatc cgttaagaat gtgttttgcg tccaacgatc
      721 cgcatgtctg accaaggcta aacttttgac cttctctgat tgttcaaagt ccttattatc
      781 aaagtaagcc catccacatt tttcataaag aaagctagag attttatcac ttacttgagt
      841 aagtgcagaa ggatccaaca gaccagacaa agaaataaac ttcgtaatct gcttgttctc
      901 caaagtcgga tcaagatgat cagctttttt aatttcttca ttttgaagtg ctaacaaata
      961 ttctccacta atttgtgcac ctgccgaatt tccaccaatc gaaatattcg accaatctgc
     1021 ttgatctttt atctcttttt tagctgcttg aatggcttcg ttcaattgga taactggtgt
     1081 aggatgtttt gcttccggtg atcgttcata atctatattt acgatgattt gctgcgtgtc
     1141 tgaacataga tattccaaat aatcacgaca atcattttta tcccctccta catatgcgcc
     1201 tccatgcatc cagaaaagaa tcggtaattt tttcttttcc actttcggtg tgtagatatc
     1261 catattatta tttttgtact ttgaaggata ggatatttct ttttttatat cgatttgttc
     1321 gattttttgc tggaaatcat ctggtcgggc atatacttgt tctttttcac ttgtgtcaaa
     1381 ttgttttcta acaagcaagc taatcggctt tggtgtgatt tgattaatca aaaatacagt
     1441 gactaaaata actacgatga taactggtac tgcaatcact attcgcttga accatttcat
     1501 tcatttaccc tctctttctt cgccaaatcc atgtgataat cgtgctaaca gataaaaatc
     1561 cacctgaata aatgagaagc gaatgtttct tttctccagt tttcggcaac attctttttt
     1621 ttgcatctgt ctttttttgt actttcactg tcatctgaat cggttgcgta tcatcacttg
     1681 attgatcctc tcgaggttca ttctttccct tattgtctgc attctctgtc ggatcttgct
     1741 tttttggtgg agctactggg atttttttgt aatagaaaat aatagttggt atcgtctcat
     1801 taaaaatccc ttcagtcggt ccttcaacat ttgctaattc gtatccttcg aatgtctttt
     1861 gctctgtcat ataattttct ccataagctc cgtaaagctg gatttcgtcc tggtctttga
     1921 gtagactttt attgttttca tccacatact ttacgtttat ctgatcatgg aacactactt
     1981 tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc aacttcctgt gcctttccat
     2041 gccctgctcc ttctactaat aaaagatctt tataccctcc tgataactca tataattttt
     2101 gcgcgttaga cacaggtact acatcatctg ctgttccatg gataatcaat ttaggcaatg
     2161 aatctgacat tccaatcgta ggtagagcag cttttgtatc caaatgcatc tcctgatcaa
     2221 aatagtattg tttcaaaagg cccataaata aatcttcatg ctcttgatcc ccaatgatgt
     2281 ctaactgatt ttttatggca tccggtagtg cgggggctac tgtctggctg tacgtaaaac
     2341 gtagttgttc atcaattgat gcaaatccgc aattttcaat aattcctttg acattgacag
     2401 gaatatcttt cgctaataca gacatcatcg tagctgctcc catcgaacca ccataaagta
     2461 aaatcttttg agatggccgc tctcgaactt cttgatttat ccagtaaagg acgtcatctg
     2521 actcatattg tccataggtc acataatcgc ctccgctatt accagtcgct ctggaatcaa
     2581 caaataataa attatacccg gccttataaa aatcattgta ttcttctgta acgatcccgt
     2641 tattccaatt ccctcgaaat ccgccatgga tcatgactgt tttatctgaa ccttgatcga
     2701 cataatatgc atatacatcc atcattttgc catcttgttt gtttagtaca gtttgatgaa
     2761 gttgttttgt atttggatag aagcctgtgt tttctgtagg cgttgtccca tcatctgtac
     2821 ctccaagcat cgacatcaac acactgtttt ttgcaaaagc gatatcgtac aatagataag
     2881 tagcaatcag atgtttgata atctctttat ctacaccata atcattaaca gcaagctcag
     2941 tgactaataa ctctattaat tttgacttga tgtcttctgt tggtccatta agtatttctg
     3001 tcatctgttt accgaagtaa ttcaaatatt ctttaccatt gaaattttca cctttaccga
     3061 tatcatcatt ttctgtccca ttctctttgt cacttacttt agcagaagag gattcgtcgt
     3121 ttgacatttt tgtagctgtt gcttcttctg tttgttggtt atctgtggta gcggtcgttt
     3181 cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc agtatttgtt tctgtttgtg
     3241 aagtactcga tttcgtttca tttccagcat cagtagtttc tgtggtttct acctcccgag
     3301 attccgattc ggaagaaatg ctctctccat atactgtcca ttgcgggacg cttgtactta
     3361 cagcaaataa gatgaccaac gctcccttta atacttgttt tgttctcaag ctattcactc
     3421 ctttttatgt ttctaggaga attatatgta aacgcaaaca ttttttatac aaaaaataaa
     3481 tggtttatcc cataaaaata gaacaggatt attttctcat tttacgtaat aaagcagaat
     3541 ggataaacca gtatccattc tgcttttttt aaacgaagaa acaacttatt gctccaaaaa
     3601 tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg attgtttcct ttgctttcat
     3661 aaaaggatta tcataagttg tcgttgctcc tctacacatc ataagctggt atcctaatcc
     3721 atgagcaaac ttcacagtcg tatctataca atactctgct tgtgccccac ataattcgat
     3781 cgaatgacag tccaattcag ttaataaaac actcaaatta gtcttataaa atgaattcgc
     3841 atgggttttt ctcacaaaat aactgtgctc aggaatagat aattccgaca atagctccca
     3901 tggttttgtt tgtggcacta attgagcatc ttcgtgttga acgaaaataa ctggtttccc
     3961 aatcgtttcg tatgttttga ttcttttatt cactaatgct gtcactgttt ctaattcgtg
     4021 tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg atcactacta ttgcgtcggc
     4081 tgtgtttgac atacttgtgc cttctttctt ttttcttgat ttaatagtat ttcttatcta
     4141 gctagttcca ttatacgtga caacattttc ttattttaca cctctaaatg gcacttttcg
     4201 actatttctt catgttatta tttcataaat acttagctat tggaggaatg tatgatgatt
     4261 aaattgtata caggttctgg ctgctcgtct tcaagacgcg caaaacgttg gcttatcgtc
     4321 catgatatac cgtttatcga acagaactta attcgtcaac cactaaccaa aaatgaattt
     4381 atccacttac taaaattgac aaacaacggc gtgaccgaaa tgctctctac tcattctatt
     4441 agttataaaa atcttgttca cagaatagat gaactcagtt tgagtgaact atatattgag
     4501 attcgtacat ccccttcttt attgaaaaaa ccgattatct acgaccaatc tgcaaaactt
     4561 ttgactggat ttagcgaaga taaaatcagg atgttccttt ctaaaactaa aagaaagcaa
     4621 gagctcaacg aactcttgct ttctggtcat cttgtcgatt ttaaagaaaa taatcttttt
     4681 gcaatgtctt aattttggca taaacaatct gcttttctta attaacgaaa tccaattttt
     4741 ccaatatgga tctatttttc tcattcctga tttttatgat ttcagctttc agacgcgccc
     4801 aatcattttt tgtaggttgt ccattccagt aaaatatctg tgtattcgtt tgatcatatg
     4861 cctttttgtc gataggataa tcgctaatga caatatcagg taactgatca gcggatgcac
     4921 tatattttat tgggcgttca gtcatagata atatcctttt ctttgcccat aattcctgtg
     4981 ttttaccata ctttgattga atatagaata tgacaggttg ttcgtgtttt ataaaaatat
     5041 ccctgactaa catacagtat tgaaaaatca accgtctgtt cttttcataa taagtattaa
     5101 gctctggaat tctgcgagct accccactaa aaaactgttg catgattgga tacatcactg
     5161 gaaatatatg ctgaaaatct ctgtcttctg ctgattttcc aaaagcatcc accttctttt
     5221 ttgtcccaaa aacccttgaa tacaaatgga tcattgccaa attgacatat agataatttt
     5281 tttctgaagt tgtaaaacaa atcttgaagt ttttttcaaa attttctaca atagacgatt
     5341 caatccagcc ccaactttct tgtactttat ctggagtatc caacaaatct tcttccaaat
     5401 aggtgttcat tacaccaaac ataaaataaa ggtaagcttg ctctgttgga gtgatgtgat
     5461 tcatcgacca gaagtttgct tcaaaaaaag gctctagtat gtaatgcgta aactcttctt
     5521 ttgttatcaa tggatgcaca aatttgttca tctcttctgg gacatacgta atcgagtggc
     5581 cttgtttcat tcgttgcaac gaaattctta gtaggctgat ccatttcttt ctatctatac
     5641 tacgcgtata tggcgcatgt ttcgcaaatt tttctaatag ttgacgttta ttttttatac
     5701 ttgataatat atcagaatga ttttcgtcga atgactgcca atacatcaag tgaaaaaagt
     5761 atcggatacg gtattcttct ccttctatcg gattaccttt tttcaagctg caagataaat
     5821 gatagttttt caaaatagta ttaagttctc ttaattttct ttgaatcgta cgcaaactta
     5881 tataattttt tgactaagat tttctaagga ttgataagta cctttgtaaa gtgaatccag
     5941 taaaatataa ccaatcgttc cctctaaaag tatagaataa actttactca gtaaaaactg
     6001 agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg
     6061 atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta
     6121 gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata
     6181 gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca
     6241 tactcctttt tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata
     6301 gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt
     6361 gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa
     6421 aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt
     6481 cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac
     6541 tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat
     6601 ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt
     6661 atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt
     6721 tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg
     6781 taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt
     6841 gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
     6901 ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     6961 gctcgtgttt tctgtgctac tttcactgct cgtactctct gtactgcttt caccgctcgt
     7021 gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt
     7081 ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa
     7141 atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt
     7201 atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc
     7261 tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac
     7321 gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg
     7381 atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat
     7441 tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt
     7501 cttcatttct atcactctcc atataattac cttctatttg ataaaggaat tatataaaat
     7561 gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg
     7621 cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat
     7681 cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt
     7741 tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat
     7801 agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt
     7861 ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt
     7921 ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag
     7981 tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg
     8041 attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc
     8101 aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac
     8161 tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa
     8221 ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac
     8281 aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc
     8341 tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg
     8401 tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag
     8461 tgcattattg acaagaattc ctaaaacaaa accaactaag actattaaaa cagatccaat
     8521 caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt
     8581 ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac
     8641 gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg
     8701 ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat
     8761 gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat
     8821 tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa
     8881 atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata
     8941 cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact
     9001 cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa
     9061 actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga
     9121 taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct
     9181 attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt
     9241 ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat
     9301 gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact
     9361 aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag
     9421 ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa
     9481 atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa
     9541 aacaatccgt aaagtgatga
//
LOCUS       DACDHL010000094         9489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDHL010000094 DACDHL010000000
VERSION     DACDHL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.095"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..486
                     /locus_tag="IV228_002363"
     CDS             <1..486
                     /locus_tag="IV228_002363"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8718217.1"
                     /translation="GTKKELLENLIKAYQLQNVIFLLGGQSNPYKYMSRADLYVMPSI
                     TEGFPNALVEAMSTETAVLCADCKSGPREILSNRTLDTICENIEYAESGILVCPMTDS
                     RNYSPQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTF
                     N"
     gene            524..1615
                     /locus_tag="IV228_002364"
     CDS             524..1615
                     /locus_tag="IV228_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8718218.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(1683..3161)
                     /locus_tag="IV228_002365"
     CDS             complement(1683..3161)
                     /locus_tag="IV228_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP8718219.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(3427..4175)
                     /locus_tag="IV228_002366"
                     /pseudo
     CDS             complement(3427..4175)
                     /locus_tag="IV228_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            4318..5268
                     /gene="pfkB"
                     /locus_tag="IV228_002367"
     CDS             4318..5268
                     /gene="pfkB"
                     /locus_tag="IV228_002367"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8718220.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            5250..6713
                     /locus_tag="IV228_002368"
     CDS             5250..6713
                     /locus_tag="IV228_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8718221.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            6710..7168
                     /locus_tag="IV228_002369"
     CDS             6710..7168
                     /locus_tag="IV228_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8718222.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            7165..8139
                     /gene="lacD"
                     /locus_tag="IV228_002370"
     CDS             7165..8139
                     /gene="lacD"
                     /locus_tag="IV228_002370"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8718223.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(8318..9268)
                     /gene="yidC"
                     /locus_tag="IV228_002371"
     CDS             complement(8318..9268)
                     /gene="yidC"
                     /locus_tag="IV228_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8718224.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         2965 a         1567 c         1928 g         3029 t
ORIGIN      
        1 gggacaaaaa aagaattatt agaaaattta ataaaagcat atcaactaca aaacgtaata
       61 tttttgctag gtggccaatc aaatccttat aaatacatgt cacgtgcaga tttatatgtc
      121 atgccatcta taactgaagg ttttcctaat gcattagttg aagcaatgtc tacagaaact
      181 gctgtcttat gtgcagattg caaatctggg ccaagagaaa tattatcgaa tagaacgcta
      241 gacacgattt gtgaaaatat agaatatgct gaatcaggca tattagtatg tccaatgaca
      301 gacagtagaa attattcgcc ccaatatgaa gaatgtgata aaaatttagc gaatgctata
      361 gaagaattga ttgaaaacga taatttgagg aataagtatg caaaagcagc aaaaaagaga
      421 gcagaactat atagttatga tgcctttgaa cataacttgt tgcaaattat gaagacattt
      481 aattaattca tatgatgaaa taaaaataag ggagtataac tagatgctga aaaaaaatac
      541 atctttacag aataaaacaa tattaataac tggcgcagcc ggttttattg gatcaaattt
      601 ggttcttgag ttattacgta ctcagtcatc aataaaaatc attggcattg acaatatgaa
      661 tgattactat gatgtctcta ttaagaacta tagattatca gagatagaaa cggaggcaaa
      721 gaaccatcca ggatctactt ggaattttat caaaggttct attgctgata aagcaacaat
      781 tgatttcctc tttgataatt ataaaccaag tatcgtggtg aatttagcag ctcaggcagg
      841 agtccgttat tcaattacta atccagatat gtatatcgaa tctaacataa taggattcta
      901 taatattctt gaagcttgtc gtcattcata tgataatgga gaatctggtg tggaacattt
      961 agtgtatgcc tcatcgtctt ccgtttatgg tactaataag aaaattcctt attctacaga
     1021 ggataaagta gacaatcaag tatcgttgta tgcagcaact aagaaaagta atgaatcaat
     1081 ggcacatgca tattctaaac tttacaatat tccgtctaca gggctacgtt tctttacagt
     1141 atatggtcct gctggtcgtc cagatatggc ttattttggt tttactgata aacttcgtgc
     1201 tggtgataat atccaaatat tcaattatgg gaattgcaag cgtgacttta cctatataga
     1261 tgatattgtt gaaggagtaa agagggttat gcagtgcgct ccagagaaaa ttgatgggga
     1321 agatgggcta ccaatcccac catatcatat ttataatatt ggtaacaata cgccggaaaa
     1381 cctactagat tttgtagata ttctacaaca agagctaatt gcagcagggg tgctaccaga
     1441 agattacgac tttgaatctc ataccgaact agttccaatg caaccaggag atgtgccagt
     1501 tacttatgca gatactagtg cgttagaacg agattttgaa tttaaaccaa gtacaagttt
     1561 gcgtgacggg cttcgtaaat ttgcgcagtg gtataagtct ttttatgaag tataattgta
     1621 tttttttact tataaaagtt tatagaaaca cctcagacac tacaaatctg aggtgttatt
     1681 ttttaatctc gttgaaataa atctctggta tatacttttt cttttacatc atcaagatta
     1741 ctatcgtaac gattagcaat aatactgtga ctgatatttt taaaatcatc taaattattt
     1801 attacttttg aaccaaaaaa tgtagtgtta ttttcaagtg ttggttcata aattacaata
     1861 gttgccccct ttgctttaat tcgcttcata actccttgaa tagatgactg acggaagttg
     1921 tctgaattac tcttcattgt aagacggtat acacctatga ctacctcttt ttctttttct
     1981 gcattccacg aactgttagc ctcatacgtg ccagcaagtt ctaacactcg atctgctata
     2041 aagtcttttc ttgtacgatt actatcgaca attgcactta tcatattctg aggaacatcg
     2101 ttatagttag tgagaagttg ttttgtatct ttcggtaaac aatagcctcc atatccaaaa
     2161 gatggattat tataatgtgt gccaattctt ggatctaagc aaaccccgtg aatgatttct
     2221 tgtgtattta gtcctttcat ttctgcataa gtatccagct cattgaagta agatactcga
     2281 agtgcaaggt aagtattagc gaataattta actgcttcag cttctgtgaa tcccatgaaa
     2341 agagtttcga tatctttttt gattgctcct tcttggagta aatctgcaaa agtgtgagag
     2401 gcttctcgta aagttttgtc agtttggtcc gtgccgacaa taattcgcga aggatataga
     2461 ttgtcatata atgccttaga ttctctgaga aattctgggc tgaatagaat atttttagta
     2521 ttgtattttt tacgaatact ttcggtatag cctacgggta ccgtcgactt gatgaccatt
     2581 attgcctttg gattgacttc taacaccaat ttgattactg actctacagc agaagtatca
     2641 aaataatttt gtttactatc ataatttgtt ggagcagcaa tgacaacaaa atcggcttct
     2701 ctataggcgg ctgcaccatc agtcgttgca attaaattaa gatttttttc agctagatat
     2761 ttttcaatgt aatcgtcttg tattggagat ttcttattgt taatttgttt tactttttca
     2821 ggaagaatgt ctactgctgt tacctgattg tgctgggcaa gaagagtagc aatggaaaga
     2881 cctacgtaac cagtaccggc tacagcaata ttcttcgact gatgttttgt tctgtttgat
     2941 ttatttgatt catttataaa tagatctgtt ggttcaaaat ttagaacttc tgctaatgat
     3001 tctaattgtt gtattgacgg gataaaatcg agagattcta ctcgactaat taaggcacgg
     3061 ttgataccag tagctactgc aagttgttgt tgtgtcattt tcagagattt tctagaatca
     3121 ataatgattt gagctaaacg tttttgagat aactttttca tgatgcactc ctttttttgt
     3181 tgctaataat aacaaacaat tttttcttct ataacagaat agcttattat taataaaact
     3241 gtcaattttt ttattgatta aaaagatatt ttttgatttt aagtattttc aattattttt
     3301 tgttacttaa aataacttta tagattgtga ataactttat acataaaaag tttacgtgag
     3361 tccatacaat ataaagaata aaaaatgtga tgttttaaac tgtaaccaac cataataaac
     3421 tgcttctcaa ttctccctca tacgcaatac ataggcgtca agcaggattc tcgcgatgat
     3481 ctggagaggc aagcgtgaac gtacttcata atcagggaaa taagaacctt ctgatttgaa
     3541 acctacgaga tttatctcgc aataatcttt gagtgtagag gaatgattgg ctgtaactag
     3601 aatcgaacca atctctgcat catagcagtt ctttgcggcg gtgacgagtt cttttgtctc
     3661 gccgttcaaa gaaacaaaaa taacgctgtc tttcttagtt agtttccgac tgatgctttt
     3721 gataatctct ggatcagtat gcatttgaca attttttcct gttaattgaa gttttacgag
     3781 catctcttga ccaatcattt ctgaaaatcc tcgggcaaag acaaaaaccc ggtcagtaaa
     3841 tttgattctt tgtattgcat cttcaatgtt cccgatttcc aacatattga tcgtacgtac
     3901 cacttcttgt tcatttttta gaatggctcg acggatacct ttgtctactt tatctaaaaa
     3961 gaaaaattga tgttcttgtt ttctccttct tttagatggt gcttaaacga agtaaatcct
     4021 tcatagcctt ttttcttcat tgtccgcaca atggtggctg ttgagacatt tgcttgttca
     4081 cttagtttca cgatggagta gttagggatc tctaggatgt ggtgttcgat aaagttccag
     4141 aggtactgtt ctgtttcact taatttgtga tccatattct ttccttcttt cactaaaagt
     4201 tagttaaaaa atagcacgat gtgtttataa atgtaaaggt tttctttgtg ttggaacgat
     4261 taagaaaaca ttttctcttt cgcgtatttc ataatagatt caaggaggtc attggttatg
     4321 tctatctaca catgcacgat gaatttggca attgatctat ttattgaaac agaggagatg
     4381 catcctttta tggtaaaccg tacaaaggag gatgacattc aggcaaacgg aaaaggagta
     4441 aatgtgtcac tagtattaaa gatgctagat ttcccaagta cggcattagg tttttctgct
     4501 ggtttcacag gaaaatatat agaagactat ttacagaaga aacaaatcca aacagagttt
     4561 attgaagtgc ctggtatgac gcgtatcaat gtatttacac aagtcaatca aacgagagaa
     4621 gaatacaaat tagtgaatca aggacctgaa ataccgcagg cagctgtcca taatttttta
     4681 aatcaaataa gagcattaca agcagaggat tatctttgtg tttctgggag tttaccaaga
     4741 ggaatgtctc cgaaggtttt agtggaaatc agtcggattt gtcaggaaaa ggagattcat
     4801 ttgattattg atagcagtga tcaagaggtc atggattgtt tgccctatcg accatttttg
     4861 ctgaaaccga atgaggaaga actttcttct tggttcggac gaaaaatggt tacagatgaa
     4921 gattactttg tttatggaca acgactggta gagtcaggtg cagaaaatgt tttgttatcc
     4981 cttggtgaga aaggagcgat tctttttacg aaagacaggg tgattcgagg aaactcgcca
     5041 aaaggaaaag ttgtcaatac agcatgtgca ggtgatgcca tgttaggcac ttttttagta
     5101 ggatacatga atcgacgacc gcttgatgaa acgttacgta aaagcatagc agcaggcagc
     5161 tctactgctt ttcgtaaagg actgactgat tttttggatg tggaggaatt aagtaaacaa
     5221 atcaaaatca aggaagagag gttcgaaaga tgggaaaata tcaactaatt gcagcaaccg
     5281 gttgtccgac aggaattgca cacacctaca tggcgcaaga ggcattagag caagcagcta
     5341 gaaaaaaagg catcacgatc aaagtggaga cccatggaca aattgggatc gagaatgaat
     5401 taacggctga agaaatcaaa caggcagatg ctgtcattat tgccgcagac aaagatgtcc
     5461 attcagaaag atttgcggga aaacgagtca ttgatgtttc tgtcagtaca ggaatcaagg
     5521 atgcacaacg gctgattgac gaagcattgg caggaaaagg gaccatctta gcagataatg
     5581 aagctgttga tactttagaa tcagaaacga taagtggtgc taattttggt cgcagcatct
     5641 ataaaaattt aatgaatggc gtctctcata tgttgccttt cgtggtagct ggcggagtat
     5701 tgattgctat ttcatttgcc gtttggggaa tctattcttt tgatccagac catgcacaat
     5761 acaatgctac agcggcgatg ttaaaaacgg ttggcgatgc agcaatgggg atgatggttc
     5821 cagttttagc agcttatatt gcagaaggga tcgcgaaacg tccagggtta gtcgttggat
     5881 ttgttggtgg aatcgtggcc atgagtggcg gtactggatt tttagtaggt attttagctg
     5941 ggttcttagc aggttatttt atcttatttt tgcaaaagct actgacaatt ttacctaaac
     6001 aactagatgg cttgaaggca atcttcttat atccagttat tggtgtagtg tttatcggca
     6061 tcgtgatgtc tttgctggcg ggtccaatga aggctataaa tgaaggaatg atgagctttt
     6121 tagctggatt tgaacattcg aatccacttg ttcttggcgt gattgttggt tgcatgtgtg
     6181 cgtttgatat gggaggaccg attaataagg cggcatatgt aacaggtaca gctcttttgg
     6241 cacaaggaaa cacgactttt atggctggcg tgtcagtagc ttgcattgca ccaccgttga
     6301 ttactggttt tgcaacttta ttgtttggta aatattttga tactaatgat cgtaatgcag
     6361 gtttggttaa ttttatttta ggatcaactc atattacgga gggagctatt ccgtttgctg
     6421 cgaaagaccc gatccgagta ttaccaacga tgatgttagg ctcatcaatt gctgctgtct
     6481 tgacctatct gtttggtgtc caagtaccag ccccacatgg cggtttttta gtgttacctg
     6541 tagtgacggg aaaaatccaa tgggttacag cgattcttat tggttcgatc gttggtggag
     6601 tgatactagg tttgatccaa aagaaaagag taactgataa agacaagaag atggataata
     6661 aagcagcaaa tcgtacagtt atcgaagaag gaagagcagg aggcttatca tgacaatcat
     6721 tcatgaagag aacatttttt tacaacagcc attagatagt caagaagaag catttaagtt
     6781 tttagcaaag caaacattct gtttaggaat cgctcatgac gaaaaagaag tatatcgaaa
     6841 acttctagaa agagaaaaag aaggtactac aggaataatg tcaggttttg ctatccctca
     6901 tgcgaagtct caaacaatca acgaacctag tatgattatc gtcacactga acaaaggaat
     6961 tgaatggaac agtttagata gacagtcagt tgattttatt tttgcgttat ttattccaga
     7021 ttcagaagca ggaacgactc atttgaaatt attgtctctc gttgcacgat cattgatgcg
     7081 aaaagaggtt acagaacagt tgagaaaagc aaaatcaaaa acagaaatag ccacaatatt
     7141 aactaaagaa ataggtgaag atctatgaag aagatgagta tagaaaagca ggaacgtttg
     7201 aagcgattga cggatgaagc tggaattatt agcgcgttag caatcgacca gcgtggtgct
     7261 ttgaagaaaa tgatcaacca atataaagaa gctacatcag aagacatcat ccaatttaaa
     7321 cagatggttt ctcaatacct gactccctat gcctcagcta ttttacttga tccagaatgc
     7381 ggcctgcctg cagcagcgct tagagagaaa gaatgtggct tgctgctagc ttatgaaaaa
     7441 accggctatg atacgaaaat gccgggaaga ttacctgatt tattgaatat ttggtcggtg
     7501 aaaaggttaa aagaagctgg tgcggatgct tgtaaatttt tattgtacta tgacgtggat
     7561 gaaccagcag agattaatgt gcaaaaacaa atagttatag aacggattgg ttcagaatgc
     7621 gatgcagaag gcataccttt ctttttagag atcctttctt atgatgcaac tattggcgat
     7681 ccgtcctctc ttgcctatgc aaaagtaaaa ccccataaag ttattgaagc catgcgagaa
     7741 ttttcaaaag aacgttatca agtggatgta ttgaaagtag aagtgccagt gaatatgaga
     7801 tttgtggaag gatatgggca agagacttgt tatagtcaag cagaggcatc cgcgtatttc
     7861 tatgaacaat cgaaagtgac tgaacaaccg tttatttttt taagtgcggg agtcaatgct
     7921 acgttatttc aagatacatt acgttttgca aaacaatcag gttcgacatt caatggtgtc
     7981 ttatgtgggc gagcaacatg ggcgaaggga gtggcacctt ttgttaccga aggcgaagaa
     8041 aaagcgagta gttggatgtt agaagaagga aaacaaaata ttaaggaatt aagtacacta
     8101 ttaaaagaaa cagcaacacc tgtctacgtt gatagataag aaaaagttgt ttcttcataa
     8161 aaagatgctg ctgcaattag atagcgatca atcgatgatt caaattatac attcacttaa
     8221 ataaaaatat cgactagatt tctttttcaa aaatcttggt cgatattttt aattgctaaa
     8281 actgttaatg aatttttatt acactcttat ttactatcta cttccgcttc tgctgctttc
     8341 ctgcgttgcg gcctttgtta tttgtactgt tttttggttg tttgatttgt tttgttgctt
     8401 gtttgttgtc agctggtttt gctgattctg tcacgtcttt tcttggagtg acgacttgtt
     8461 ttggcgggtt ttgtttcaac tcttctgcca cttgtgcttt caagcgtggt ttcatcataa
     8521 cgtttgtgat gaatgtttgc agacagctga acacaccacc gactacccag taaagtgtaa
     8581 cgccggctgg cgcactgatc gacatgaaga cgatcatagc aggagagacg atcagcatcg
     8641 tacgcatcgt ctttttctgt tcttcaggaa taccgattgt tgaaatatat ccttgaagca
     8701 gataagcgac ccctgcaatg gccacaagca ccatacttgg tgagcctaga tcgatgccgt
     8761 aaaaagatga ttcacggata ccttctgtgt agcgagccgt aaaatacaag gcagagaaga
     8821 tcggcatttg gatcaataga ggcagacagc cgattccgcc ggtcatactc aatccgtttt
     8881 cgcgatatac ttgctgcatt tctgcttgtg ctgccatttg ttcttcacgt gttgttgcag
     8941 cttttagctt ttgttgtgct gcttcaactt gtggttttaa cgcttgcatt ttttcagatt
     9001 ggatcattgt ttttttcgat tgactgatac ctagtggcaa gatgatgata cgaacgatca
     9061 cagtcattgc aataactgcc cagccgtaag accaattgaa attctcaact agatatgtaa
     9121 tcgcttggcc cataggatgg acgagtactc gataaacgat tcctgaagtg tcagggtttc
     9181 cgtttgaatc catccgcaca cagccagata aaaagagcag gatactgaca agtccggatc
     9241 ccaataacca gtttttccat ttattcatag aatacttcct cttctataat ttttacacaa
     9301 acttaactat actaaaaaag cgcatggaat tcaatcaact tcatagaaat ttcatataaa
     9361 tattctttct ttttcgtagc gaaaaatttt gaaaaaagac aaaaaatcaa gaaagaaata
     9421 cttggaaaga aaagagtgat tccaagtgtt tctttcttat ataaaaaact aaaaactttt
     9481 ttgttctgt
//
LOCUS       DACDHL010000095         9225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDHL010000095 DACDHL010000000
VERSION     DACDHL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.096"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            436..735
                     /locus_tag="IV228_002372"
     CDS             436..735
                     /locus_tag="IV228_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718225.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(832..1266)
                     /locus_tag="IV228_002373"
     CDS             complement(832..1266)
                     /locus_tag="IV228_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8718226.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1498..2208)
                     /locus_tag="IV228_002374"
     CDS             complement(1498..2208)
                     /locus_tag="IV228_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8718227.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2198..3058)
                     /locus_tag="IV228_002375"
     CDS             complement(2198..3058)
                     /locus_tag="IV228_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8718228.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3063..3701)
                     /locus_tag="IV228_002376"
     CDS             complement(3063..3701)
                     /locus_tag="IV228_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8718229.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3716..4015)
                     /locus_tag="IV228_002377"
     CDS             complement(3716..4015)
                     /locus_tag="IV228_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8718230.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4058..5542)
                     /locus_tag="IV228_002378"
     CDS             complement(4058..5542)
                     /locus_tag="IV228_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8718231.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5562..6023)
                     /locus_tag="IV228_002379"
     CDS             complement(5562..6023)
                     /locus_tag="IV228_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8718232.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6041..7108)
                     /gene="ulaG"
                     /locus_tag="IV228_002380"
     CDS             complement(6041..7108)
                     /gene="ulaG"
                     /locus_tag="IV228_002380"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8718233.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7326..8096
                     /locus_tag="IV228_002381"
     CDS             7326..8096
                     /locus_tag="IV228_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8718234.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8369..9196)
                     /locus_tag="IV228_002382"
     CDS             complement(8369..9196)
                     /locus_tag="IV228_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8718235.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2838 a         1908 c         1460 g         3019 t
ORIGIN      
        1 gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga atatccttta cttggttcat
       61 ggaaagaagc tgctcctttt tccaaaccat gtgccataaa aataaagaac gagctagaaa
      121 aagaactgac ttgtggtaaa gctgttgtag atatcatcga aaagtaaatc caatgtatag
      181 aaaggaagat cacacatgaa aagcgacgta caaattgcta agattataga attagaagaa
      241 gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg tataccatat gattatttca
      301 atatgtatcc tggaattgga gatctcgtag agctatatca agatgaatca aatatacttg
      361 tcattcatca tgaaatatag agacttataa aattgtttat ttttataatg tatcagtata
      421 gaaaggaaat aaaacatgga ttatactgtt gaatttgaca gtgaactact taagcaattt
      481 ccacaagaag aacatttcat gtataaagaa gcaatttccc acaaagtgac agagtatttg
      541 gagcataatt tttttcgtat aaagccagtg cgtactcgtt cggcaaaagg aatatacgag
      601 atgaaagtca aaattaataa aaaaaattat cgcatagcat ttgctttaaa aaaaaatcat
      661 gtgttcattt tttatattag ttcgaatttg caaaaaattg tatttgataa agaggtgagt
      721 aaaaaaataa aatagctgag cattggagga tagattgagt gatactaagt ctatcctctt
      781 gtttttctaa agaactaagc tagaattttt tcttttgtac gttattatat gttatttgtc
      841 attaccaaaa agcacctttt ttcttctagt atctatgaca taagaaatac cacccatttc
      901 ctttacttga acaaatccta ctgattcata aaattttttt gttttctcag tatcatctgt
      961 caataattcg atttggactg gtccataact agtgatcttc tccaaagctt cattcaaaag
     1021 cactttgcct attccttggc gctgatacct cggatgaatg aggatatctt gaataaatgc
     1081 gatcgtcgca aaatcagtga tccaacgaac gattccgata atcttccctt ccccattacg
     1141 ctttatcaga gattcagatt gttcaaatgc tctagctaac cttgcagtgt cttttgtgta
     1201 attcgtccaa ccaacgctct tatataattc agaaatctca tcgatcataa gggaatcaat
     1261 attcattttt ttcttccttt cttatacaat tctccctcta ataatacagt tctttgacta
     1321 tttaatctat cacatataac tctcctgtaa agattctctc gattccttaa aattcctctt
     1381 tccttttaga atgcatactt tttgataaat aaaaaggttg tgataaattt tcttcacaac
     1441 ctctgtttct ctctatcaag tgttatcttt taattttcta cccttaccct agcatctcta
     1501 ttgcccgtag tatgcatact ttccatgttt gcgaaaataa tgtttatcta gtaaatattg
     1561 cgggatttca catatattgg ggttgatcag ctcgttttct ttggccatca gacaaatttc
     1621 atctaagatc aaactgtttt ctactgcttt cataggtgaa tccccccatg tgaacggtcc
     1681 atgtccatag acaagtactc cagggacttc gtttggatct aattttcttt ccttaaacgt
     1741 ctcgacaatc acattccctg tatgaacttc gtaagcttct cttacttctt ctttcgttaa
     1801 ttggcgtgta caaggaacct taccataaaa agcatcagca tgagtcgtgc cataagctgg
     1861 caaatcgcgt cctgcttgcg cccacatgac ggagtgcgtg gaatgagtat gagtaatagc
     1921 agttatatct tcaaaagttt gatacaaaac aacatgtgtg gggagatctg aagatggttt
     1981 taagctatct tcttctatta tatttcccga caaatctgtt accaccattt gatctgcttg
     2041 catgcattca tatctgacac cgcttggctt aatgacgatg actcctagct cacggtccac
     2101 ttcactcaca ttgccccaag tcaatttgac taatccgtat cgcggcaatg ccagatttgc
     2161 ttcaaggaca cgttgcttca ttgctgcaat tttttcttca caagacatca tagccagcct
     2221 ccttcaattt agaacttaag tagtcttttg cctgctggat ttctcttcga aagtcttggc
     2281 ttttttcact ccacatttca atcagaaatg ttccatcata atctagacgt ttcaaatgtc
     2341 ttaataatcc aagaaaatca acacaaccgc taccaaaagg aacatcacga aactggcctg
     2401 aatattcttt tgttacaggg taagtatctt ttaagtggat agcagtgata taatcgatcc
     2461 ctttctcaag ttctcgagcc ggattatttt ccggccatgc ggacaaattg cctaggtctg
     2521 gatatacttg tagaaaaggt gaatgaatct gttctttgat ttctaaaaat tttgaaatgg
     2581 agttcataaa tggatcatcc atgatttcca cggaaagaat gatgccatat ttagaagctt
     2641 cctttacgct ttcttttatt ccttcgataa agtcttctct ggaattcatt gatttctctt
     2701 cataataaac atcgtatcca gctatttgga ttactttgac gcttagatta tgcgccaaat
     2761 ggattgcttt tttcattagt tgtttcgctg cttttttctt ttctggatca gcagaaccaa
     2821 acggaaatcg ccgatgcgca cttaaacaaa tcgaatggat acgtacaccg gagctgaaca
     2881 tctcttctct cagctcagct atcttttctt cagaccaatc taatcgtgct aaccgctcat
     2941 ccgtctcatc aatcgacatc tcgataaaat cgaagtccat ttcttttaca agggaaaaac
     3001 gttctttcca tgaaatatct ttgggtaacg ctttttcata aatcccaatt tgagccattt
     3061 ccttaccccc agatacgaat gatctcatct ttgaagtttt gtgcggcctt catcggatca
     3121 tttgctgctg taataccacg accagtgata aaggtataca catcgatccc ttcaaataag
     3181 tgtaatgtgt gtggatttaa tccaccagtt acagaaacat taaagcctag ttcaatcagt
     3241 tttttgatct tcgataaatc tttttcaccc caagtttcac cagataacag cgcatcacgg
     3301 ctttgatggt agattacctg ccggataccg atgttatgcc aatccatggc ttgttcaaaa
     3361 gtccaattgc cgtagagttc tacttgcaat tcacctattt ctttttgtgc tgcctccata
     3421 gttggaatcg ttgcacaaca aatgactgtc atccagtctg ctcctgcttt tgcaacatta
     3481 ctcgccactg ttcctccagc gtctgcacat ttcgtatcag cgactagttt tttatctggg
     3541 aacatactgc gaatacagcg aatcgctttt tgtccttcct gtaagcaaag aattgtgccc
     3601 acttcaataa tatctactac atcaccaact ttcataacat ctcctaatgc atgttctaag
     3661 tcattgtgat ctaacgcaat ttgcaaattc ggcctgctca tcttagttcc ttcctttact
     3721 tgtttttttg gtaccacttt gtttcagcaa atttttcact gatttctttg actgacatca
     3781 catttttgat accgataact gtaactcctt ttttctctgc ctgagtaaac atattcaaaa
     3841 aattgactgg cgtaaaaact acatcgtatt ggccagctga actttttccc tctgaaatgg
     3901 cacaatgatg gattttggta attggtacgt ttagttcttt cattgctttt tccacacttc
     3961 tcatcatcat caagcttgtt cctgatccat ttgcacacga tactaaaatt ctcatcttaa
     4021 tccctccatt atgagtgttg tctaatcaga ttttctttta tactctctgc ttttctgcat
     4081 atgtgtcata gtcttctaca atcaaaaaat aaccatcagg atttttccga tattgtaact
     4141 gcggaatagc caataaacca atgacaacta cggcaattcc agcataacct aggactttca
     4201 tgattactgt aaacaatggc caagctgttg cccagtcgaa catcccgata taaccaccgt
     4261 atgttgacat cccaatccat gtagcgaata atgctgaacc gcctacttgt atgattcctg
     4321 aaagaaacgg gaacaggcaa gcggctttga atccacctcg gttattggca taaactgcta
     4381 taacggcatt atcaaagaac aacggaatga aaccggctat gacgatcgtt ggtgatttca
     4441 ataaaatcag taaaccaatc atcaaaaact gtcccaaagc accaaacaag aacccaatcg
     4501 ttaccgcatt tggcgaacca aagccaaaag tagcagctac gtcaattcct ggaacagctc
     4561 ctggtaaaat cgtgtttgag attccttgga aagattgagt tagttcatcg acgaatgtcc
     4621 gaacacctaa ttgcaagatc gccagatata ccgcaaaatt taaagaagtc gtcataatgt
     4681 agaataagaa actctgccct tcttgcatga attgcgcttg aatcaaataa tctttgccta
     4741 gtacaatcag aatgatccca aagaaaaata gcatgagtaa cgaagtagca accatattct
     4801 cattgaaaat cgacaagaat cctggtaatt tgatatcttc aatttttttg tcttgcttga
     4861 ttgatctatt tttcttttca tcccgctttt tcatccactc agctaatttt gcaaatataa
     4921 agattccgaa catttgctga tgtgccactg caaatccggc accttctgtc aattcctgcg
     4981 tgattcctac tgttaagtta gaagctactg tccaataaca acctaaaatc agtcccataa
     5041 aaatcaatgc ttgtatccta cctagatcag gaaagcaaaa cagtaaaatc caaaaagcgg
     5101 tagctgcttg ttgaacctga acgtttccgg tagtgaaaac ggaacgcagt ttcgtatatt
     5161 tctgaaaacg gactaataag atatttaaaa caaatgcaat cagtaataaa atcatcacat
     5221 ctccaaaggt acggccaaat gtctccatta atccagcatc cacagcattt tgtccaaaat
     5281 aaggatctgt aaccatggca tccaaattga accgttcctt tagtccaacc aagattggcc
     5341 gaaaattatt gaccaaacca ccagagccaa cagtcaaaat aaagtaaccg accgttgcct
     5401 ttaagaatcc agcgatgcat tcatgaagcg ggcgtttcag caaaatatag cctaataaca
     5461 cgataaaacc aatcaggtat gctggctgcg tcaagatatt tgttgcaaaa tacgtccaaa
     5521 tgcttaacaa aaattccccc atcttttttc tcctctatca attaattata ttctgccatc
     5581 acaacttggt aatcatcgat ggagttcgta gagatgagac gctcaaccaa tccatctgtc
     5641 attaataatt cggataattt ttggatattt tctagatgtt cttctggatc ttttgctgcg
     5701 agtgtaaaaa acaaactagc cgttttctcc tcattctccc cttcactttc aaaacggatt
     5761 ggctgtttca acttggtaaa tgaaatagct gttccaaaaa caccatcact tttttctgaa
     5821 gaatgcggca ttgctacttg aggtgcgatc acaatataag gaccgtattt ttctacacaa
     5881 tggatgatct cctccacata cgtttcatca ataatttcct tatcgatcaa tacaagacag
     5941 ctttcggcga tagcttcacg ccaatccgtg atttctttat ctgggtaatg aatcaattga
     6001 tggtcaaaaa agtatttcag caagaaactc cccttctttc ttataaaaac gacggaaacg
     6061 gtgtatcatt ttcaatcgag aagacatctt caaatcctcg ataaaaatta aactcaagat
     6121 catctttgtt tgtcggatag atgaatttcc cgcctacttg ccagatataa ggtttaaatc
     6181 catacttcag gcgatccttt ttgaatttcc aaatttcagt gatctctttt ggatcagcca
     6241 taaaattaga ccaaatatcg tagtggacag gaatcactac ttttgcattc aatgactccg
     6301 ccatgcgcag catatcaaca gaagtaactt tgtctgttat ccctcgtggg ttttcaccat
     6361 aggcccctaa acagacatcg attttgtgtt cattaccatg tttggcaaat aaattggagt
     6421 aatgtgaatc tcctgcatga taaagattcc ctcctgatgt ttcgaataaa tagtttactg
     6481 caatttgatc catctcctga ggcatttttc cttttaatac ctcttctgga tcttcacaag
     6541 tgaccaacgc tgtccgatca aacgcttcta aagctactac attgatgtct ttgattttga
     6601 ctgcatcacc tggctttact gtaatcgttt tttcttcagg aattccccat ttttgccacg
     6661 tgcgaaccac ttcttttggc ccaatgaatg ttgcttcagg acaattttga tgaactgctg
     6721 cggctgtatg gatatctaga tgatcggaat ggatatgtgt aacgattaaa gcgtctacat
     6781 tttttatcgc aaaaggatcg attacgaaag gctgagtacg taaattaggc tgcatatttt
     6841 gaacgccact catacgcatc atctgatggc cttttttcat catgccattc ccatgactac
     6901 gttttcccgt cccacaccat agatcacata aaatattcgt tccttcatga cttttcagcc
     6961 aaattcccgt acagcccagc caccacatag caaaagttcc aggatccact acttctgttt
     7021 cgatttcttc attcaagtat gtgccccact caggaaatgt ttctactaac cacttttctt
     7081 ttgttacttc attgacagtt ttactcattt gcttgctccc cccatatcgt tgttccaact
     7141 tacaagtaaa tattaatgtg tatcagcatc catgaaaacc cttttatatg accgtttgtt
     7201 ttttatatga ttttctttca gtaatcaatg attgtttcag tataataaag acagatatga
     7261 atgtttaaat gaacaactag tcaaataaat ccatggatca agatgtccct caggaggcaa
     7321 aagtcatgaa aaactcactt gtcaatattg aaaaacgtca aaaagaaatt ttggaactgc
     7381 ttcagcagac tgaacaacta tcaactttcg aattagctga atcccttgat gtatctatta
     7441 gtacgatacg aagagattta aatttattag aagaaaaaaa cgagatcata cgcaattacg
     7501 gttattgttc ttataattac aaaaaccaga ctgattttga tcaatctggt cctgaaagga
     7561 ttaaacaagc gattgccaga gaagctagta aatacattaa cgattatgat acgctgttta
     7621 tcaattctag ctcgactgcg ttgaaaacac tggattttct tcaatctaac catttgacag
     7681 tcattacaaa caacgtgaaa atcaattact ctccgcacaa agataattgt agttatattt
     7741 taactggtgg agagatgcgc tttccaaaag aagcactagt gggcgacatc gcattaaata
     7801 cgatttcttc agtcaatgca gacatttgta tcattggctg tagcggagtc gatgtagtac
     7861 atggggtcac aacaaagata ttaaatgaat caacgatcaa cgaaatgatg gtcaacaaga
     7921 caatcaaatg taagatttta gtcgctgatc atcgaaaaat cggactgaca tcaaagttca
     7981 agattgctga tatttcagtc tttgattatt tgatcactga taaattttgt tctgaaaaag
     8041 tattagagga gatcagaaat acggggataa aaaccattca agtagattca ttttaagaac
     8101 caggacgtaa atatgtaaat aggattatca aaaaaatgaa taacaaattt atcgataaat
     8161 gaaaaacacg ctacgctcaa attgataaag gaatcccaag atgtatccat ccaatcgata
     8221 ccatcataat ctattgcttc ttttataacc gttatatgtt gcctaaatag gaatcaaaca
     8281 atatcgaaaa aaggaggtat gacaaaagtc acacctcctt ttttcgaata aacggtattc
     8341 aaatcagaac actgatttca caaccacttt atttcaggtc gttttcaatc acctttaccg
     8401 cgtaatcatt tccgttgaaa cgcgctattt tttttgcgtc catatatctt tctaaataat
     8461 catcttctcc taattgctta tggcaagaag caatttcata aagcaagtat tccaatgcat
     8521 aagtcgattt atgtttctta ttatactcaa tacccttctc acataattgg attgctttat
     8581 cataatcctc gattttacgg taataggtgg cgatatcata atatacaatg cagaccatca
     8641 gattatcaaa atcagtctca ctcaatattt taagtgtacg ctccacataa actttcgcaa
     8701 aatctaattc tccttttgac aagtagatca ttgccacgtt tgcagctgct tgtaagtgat
     8761 ggatatctgt ttcggtcgtt tgttccaaca cttgattaaa ataaaataat gctgcatctg
     8821 gttttttcgc ataaagatga tcaagcagtc cttgataaaa taaaatttga tttaaaagat
     8881 cagaattact tactttttcc tgatcgatcg tttgtaaaat tttataagat tcttggtatt
     8941 ttgatacaga attcaatgat tgaatctcat ttattttttt cctgattttg tcttcttctg
     9001 tttctactaa tatatcggag atctcgacat tcagcttttt gcaaagcgca cttaagatat
     9061 tgatgcctgg cgacatattc ttgttttcga tattgctgac agttgcttgt tcacaaatgc
     9121 cttctgctaa ttgtacttgt gttaacccct ttttctttcg gtattctttt agtaatgctc
     9181 catctacttt aatcaaaaca atttccttcc ctttaaaaaa ttatt
//
LOCUS       DACDHL010000096         9080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDHL010000096 DACDHL010000000
VERSION     DACDHL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.097"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            330..1304
                     /locus_tag="IV228_002383"
     CDS             330..1304
                     /locus_tag="IV228_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8718236.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            1629..3524
                     /gene="asnB"
                     /locus_tag="IV228_002384"
     CDS             1629..3524
                     /gene="asnB"
                     /locus_tag="IV228_002384"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8718237.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(3719..4165)
                     /locus_tag="IV228_002385"
     CDS             complement(3719..4165)
                     /locus_tag="IV228_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8718238.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            4393..4542
                     /gene="rpmG"
                     /locus_tag="IV228_002386"
     CDS             4393..4542
                     /gene="rpmG"
                     /locus_tag="IV228_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8718239.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            4630..5148
                     /locus_tag="IV228_002387"
     CDS             4630..5148
                     /locus_tag="IV228_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8718240.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(5225..6181)
                     /locus_tag="IV228_002388"
     CDS             complement(5225..6181)
                     /locus_tag="IV228_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8718241.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            6347..7153
                     /locus_tag="IV228_002389"
     CDS             6347..7153
                     /locus_tag="IV228_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718242.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            7150..8139
                     /locus_tag="IV228_002390"
     CDS             7150..8139
                     /locus_tag="IV228_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8718243.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            8136..>9080
                     /locus_tag="IV228_002391"
     CDS             8136..>9080
                     /locus_tag="IV228_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8718244.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALF"
BASE COUNT         2745 a         1557 c         1844 g         2934 t
ORIGIN      
        1 aacgattaga ggttgtgaag aagctgtcct gcatcaagta ataagaacgg ccaaacaaaa
       61 atagcttttc ctattttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
      121 accgtttgtt cggaataagg ggctgtgaca agacttttgt cacagcccct tattgttgta
      181 cttttaacct acaatatttt ttgattcccc ctgagtacta ttttttataa atgctagtaa
      241 atcaatcttc tttggccttt tttttgataa aaaaaaatac tagatgtaag ataaaagtgt
      301 aaaaacgatt tgttttagga gggaaaacga tgtgtaccgc aatcacttat gcaacgaaag
      361 accattattt tggaaggaat tttgattatg agatgtctta taatgaagta gtcactatta
      421 ctccaagaaa ttatcgtttc gattttagaa aggtaaaaaa tttagataaa cattatgcaa
      481 tgataggaat agcagctggg gtatccaact atccactcta ttatgaggct acaaatgaaa
      541 aagggttgag tatggcagga ctgaattttc ctggcaatgc tgattataaa gaattacaag
      601 aaggaaaaga taacgttgca ccatttgagt ttattccatg gatactaggg caatgctcaa
      661 ccatagacga agctaaagaa ttattggcta ctatcaatct agtcaatatc gattttagtg
      721 aaaaactacc cttgtcacct ttacactggt tattagctga taaagaaaaa tcgatagtaa
      781 ttgaaagtat gaaagatgga cttcatttat atgataatcc tgtcggtgtg ttgacaaata
      841 atccgccatt tgattatcaa ttatttaatc tgaacaatta tcgctcgcta tcaaatggaa
      901 caccagaaaa tcatttttca aaccagatca gcttgaatgt ttatagtcgt gggatgggag
      961 gattgggctt acctggagat ctctcttctg tttctcgatt tgtaaaagcc acatttacta
     1021 aaatgaatgc tgcatcaggt gattcagaat ctgaaagtat cagtcaattt tttcatatac
     1081 taggctcagt cgagcaacaa aaaggagtat gcgatactgg agaaggaaaa tacgaatata
     1141 cgatttattc ttcttgctgt aacgttgata aagggattta ttattatcgt acgtatgaag
     1201 acagtcaaat tactgctgta aatataaata aagaagactt ggatcgtcat gaattggtca
     1261 gttatccagt cataaaagag caacagataa actatgtcaa ttaattaatg aatagatccg
     1321 tttggtatca tattttctga tacagtcata attagacacg atctttaggt cgtgtctttt
     1381 tttgctgttt tttttggaaa tagcatccac taatattttt ttaacacgaa gagaataagg
     1441 tagcctcaaa aaagaaaaca tggtaaatta gatattggga ctttattttc tgtaaaaaca
     1501 gaaactttca tcttttgttt tcacgtgacg aaattgtaag agagaaaata ttcgtccgct
     1561 atttattcta tgataagatg catatgaatg aaataaacag taattttata ttaggatgtg
     1621 aaagttaaat gtgcggtatt gtaggttttg ttaacgacaa ggacaacaaa aaaacaatta
     1681 ttaatacaat gatggatcga atcgttcacc gtggacctaa tagttcagga gaatatatcg
     1741 ataaacatgt tgctttagga ttcagaagat taagtattat tgacttagag ggcggtactc
     1801 agcctattta caatgaagat cggacaaaga tcattatttt taatggcgaa atctataatt
     1861 accagccttt gagagaagaa ttgattgcag ctggccacgt ttttcaaaca catgcagata
     1921 cagaagtcct tttgcatggt tacgaagaat ggggaacaga attattgcaa aagatccgcg
     1981 gaatgtttgc ttttgctatt tgggataatg aaaagaatga gttattcggt gcaagagacc
     2041 actttgggat caaaccatat tattatgcag aaatgaacgg aacatttatg tttggttcag
     2101 aaatcaaaag ttttctgcct catcctgatt ttaacaaaga attgaacaaa gaagcgttga
     2161 aaccttatat gactttccaa tattctccat taaacggcga aacattcttc aaagatgtct
     2221 atcgtttgcc tgaaggccat tattatacgt ataaagatgg aaaactagac attcagcaat
     2281 attgggatgc agattttgaa acaaaagaaa cgcattcccg acaagaatgg atcgaaaaaa
     2341 tcgacgaaac cgttcaagct tctattgaag cacatacggt cagtgatgta gaagttggtt
     2401 ctttcttatc cagcggggtt gattccagtt atgtaacctc tgtattgaaa ccagatcatt
     2461 ctttctcaat cgggtttgat gataaaacct ataatgaagc gattgaagcc agaaaactaa
     2521 ccgaactatt ggacttagat aatacagcag cagtgatcga cggagacatg tcgttcaaag
     2581 cattcccttt gatccagtat cacttagatg aacctgattc aaatccttct tgcgtacctt
     2641 tgtacttttt agcaaatctt gcttcacaaa gtgtgcgggt agtccaatct ggagaagggg
     2701 cggatgaact gtttgcggga taccaaacgt atggattcca tacgaattca aaattcatcc
     2761 gtgtgattgc tcaaggattg aaaaaattac caaaaggaac acgctataat ttaggacgta
     2821 aaatcggaaa aatgaaaaat ttccatggac ggatccattt atatgaatca cttgctcctg
     2881 caaaagaata ttttatcggt catgctcgag tgttcgaaga atcagaagca tcagaagtgt
     2941 taacaccaaa atatcaaaca gcaccatctg tggatgaaat catgactgtt cattacgaga
     3001 aaacagaagg aatcaaagat gaagtcaata agatgcaata tgtcgatctt catcaatgga
     3061 tgccaaaaga tatcttgtta aaggcagata aactttctat ggcaagttct ttggaagtcc
     3121 gagtaccttt attggatatc gaagtcatga aattggctca acaaatacca agtaagtttt
     3181 tattgaatca aaacaacaca aaagatattt tcagacaagc tgcgaacaaa catcttccgg
     3241 aagaatggtc gaatcgtgtg aaattaggat tccctgtgcc aatcaaagct tggttgaaag
     3301 aagaacacgg ctacgaacaa gtcaaagcat tattcgaagc agattttgca aaagaatttt
     3361 ttgatcaaga aaaaatcatg aagttgctag atgaccatca cgaaggacgt aaagaagagc
     3421 aaagaaaaat ctggacaatc ttcagtttct tgacatggta taaagtctac tttgtagatg
     3481 aaacgattcc gcaagcagaa gcaatcgact atgtgactgt ttgatgaaaa tcaaatatca
     3541 ataaaaaaga ggttttctcc taccgtttaa cactgagtga taagaaacaa tttcaaaaaa
     3601 cagctcttcc cgttttatag atattatgac ttatggttcg gagttggcag aggaacacca
     3661 ttgtttcgaa cataaggggg ttgtgacacg attttcgtca caaccccttt acatatgttc
     3721 aataatcgat ttctttttct tttgtcccat cttttttata aatgaacaaa gttgattctt
     3781 tgttttgtgc tacttgcttt cctttttcga ttgcttcttt ttttgtatca aaatggttgc
     3841 ttgccttttt tgccccttta gatttcacga tccattgttc ttcctcatat tctacgatca
     3901 catctgaatc aagcagtttt ccagcacgag gattcgtatc gtgttcatcc gtcttgcttg
     3961 gatttttttc tttttcgaaa gttcgttttt ctgattcact agcatcttca taccattttt
     4021 ttgcttgatc aatggcaatt gggatcgcac ggttatcagg gtacccttca tctaataatg
     4081 catttgcgat gtcgattgct ttttttcttg tcaatttatc taagtttttc attgatgcag
     4141 gaaagtcctt catgttccat ggcatattag tatccccctt tattaaaaaa cataatctat
     4201 ataaacaagt gtaagggtat ttatacaaaa agaaaaagat tttgtttgag aagtaaaacc
     4261 gttgtattta tcgtcaagta gttttactta acaaaatcgt gaaaaacact agcaatctag
     4321 aaaaaatcat ggtaagataa tcaagtctga gagtcatctt agaggattct tgtaaaaatg
     4381 ggagggaaaa acatgcgcgt aaacatcact ttagagtgta cttcttgtaa agaacgtaac
     4441 tatctaacaa gcaaaaacaa acgtaacaat cctgatcgtt tggaaaaaca aaaatattgc
     4501 ccacgcgaac gcaaagttac tttgcaccgt gaaacaaaat aatgttttta aagttctcaa
     4561 ttccttgctg gtcaaagaat tgagactttt tttatctcaa aacatctaaa atcataagga
     4621 gcggataata tgtttatttc catgtgggca caagataaaa atggtctgat cggcaaaaat
     4681 ggtttgttac catggcgatt gccaaatgat atgcgtttct ttcgcgaaca tacaatggat
     4741 cgaattttag tgatgggaag aaagacctat gaaggaatgg gggatctttc acttccttat
     4801 cgccatatca tcgtgttgac aacgcaacca aattttcgca cgaaagaaaa tgcggaagta
     4861 atgcattcga tcgatgaact tttgtcgtat agtcaaacga tctcagaaga tatctatgtt
     4921 tcgggtggta gtcggatttt tcaagaactt cttccttaca ctggtcggat ctggcgaaca
     4981 ttgatcgata gcacgtttga aggcgatacg tatattggga atattgattt ttcagacttc
     5041 gcccttatag aggagtatga ggggataacg gatcaagaaa atctatatgc ccatcgtttt
     5101 caaaaatggg aaagagtaaa aaatagggtt gcttcaaaag aagaataaaa tctatgcgtc
     5161 tagtttgtta ataagaaaaa aagcatctca cgactaatcg tgaagattct ttttttagtt
     5221 aaaatcaatt tagtttttct ttgatatcat tgaccatttc ctgactggca ataggtccgt
     5281 tatatagcca actagattca ggtccgaatt gcagtacatg attgtctgtc actgctttaa
     5341 gattcttcca gcttggttca ttgaacatgg cagcatcttt gtcactgttc acaaggatga
     5401 tgtagtcagc atctaattca gctagctttt cagaggaaac agcagaccag tcagaagtgg
     5461 cttttttgga gacctcttcc actagatcag gaacaccaaa ttccagatcg tcgtatacga
     5521 ttcttccact tgaccgattt ttgctgacca taaaggctga gttgttggtc acccacaaca
     5581 ctgcagctgt cttgttctcg attttatctg ctagttcttc tcgagttttc ttgatggatt
     5641 tttgataatc ggaaatgatt ttttctgcct ctttttcttt tgccaaagct ttgccgacat
     5701 cttttagttg ttcttcccat gtgacatctg ttccgttttt tacaacataa gttggcgcga
     5761 ttttgctata ttgttcgtat ttcccgcctt cgacagtagc ggaagaagaa atcaacagca
     5821 aatcaggctc aaagcttaac actttctcat aaggaaggtc ataagaaatc gttgggacat
     5881 cctttaaatc atcttgcaga taatcttgaa tggaaccact gccaacagtc cactgggcga
     5941 taggtttttc ccctagtgca atcagatagt cttctaagta actgccgatg atccgtttgg
     6001 gcgcgtttgg aatctctact ttatgtccaa gtgtatctgt gactgtatga gtactattgg
     6061 atgaagtcgt cgaattggca gttgtagctg tttctttatc tgaagagggg gagcaagcgc
     6121 tcagtactcc agccagcagc aatatgctag ataatgtgaa tacttttgtt tttaatttca
     6181 tttctttcct cctaacataa ttgagaatct ttctcattgg ttattattcc tgatatttcg
     6241 aacgctgtca accgcttcat ttggctatag atatgaaagt agcgcttgac gaaatgttga
     6301 aacagcgtca taattgagaa tgattttcag cgaaaggatg ggtgaaatga cgagattaag
     6361 agcagctgac attgcagccg gctacgaaaa caaaaaaatc attgaaaaca tgtctgttac
     6421 gattcctgaa gggaaaatca ctagtttgat tgggccaaat ggaagtggaa aatctacttt
     6481 attgaaaagt tttacccgta tactacctgt gaaatcagga aagatcattg tcaataactc
     6541 tgatatctct acatacagtt caaaagaact tgcaaaaaaa attacattat tagcacaaac
     6601 tagtgaacac cctacaggta tgacagtaga agaagtagta tcttacggac gataccctta
     6661 tcaaaaactg ttcagtggca tggacacgca agatcatgaa gcaatcgagt gggcgttgga
     6721 tgctacaggt ctagctggat ttcgtatgcg ggatatcgca acgctgtctg gtggacaaac
     6781 ccagcgtgtc tggatcgcta tggcgcttgc tcaggaaaca gatatcttga tcttggatga
     6841 accgacaaca tttttagatc cggcacacca attagagatc ctacagttat tgactgaaat
     6901 caatcaaatg aaacaaacaa cgatcttgat gtcgattcat gacattaatc atgcttcacg
     6961 tttttcagat tatttattag caatgaaaaa cggtaagttg atcacagaag gtacacctga
     7021 agaagtgatc acaaaagagt gtatgaaaaa aatatttgag attgatgcaa cgattgtcac
     7081 gcttccaaga acaaataaac cagtttttct aacctatgat ttgttgccaa aagagcagga
     7141 ggatcaatta tgaggactat gaagaaaaat agactcctgc ttttggtctg ttttgttctt
     7201 ttgattattt tttttgttgc tctttcaaat ggcgcagcga ctgtgggaaa agcagatttt
     7261 tggcaggcgt tgtttgattt tgatagatca aaacaaagtc accaaatcat acgaaatatt
     7321 cgtttgcctc gtgtggcggg agcatttctt gtagggagtt gttttgcatt gagcggtgca
     7381 ttgatgcaag gagtgacaag aaatccttta gcagattcag gcttactagg aatcaacgcg
     7441 ggtgcttcgt tggcaatggc ccttagtttt gctttttggc ctaaagcttc tgatttttct
     7501 gtgtttctaa tgtcattggc aggttctttt gctgtaactt tgctcgtatt tggttttttg
     7561 cagttttcga aaatgggcat gaatccttta cagctgattt tagctggcgt agctattagt
     7621 tcttttttta ctgctatcag ccaagcactt acccaattct ttaatctgca gcaggatctt
     7681 gctttttggt ttgtaggcgg agctgccaat atcacatgga aacaattagc agtattgttt
     7741 cctttatata tacttgctgt tttggtttct ttttcattag gaaaagcaat atctttgatt
     7801 catttgagtg aaacacatgc attagctctt ggaagtcatc ctaaacgaac gcggttgatt
     7861 gttttctgtc tagttgcagt gttagcagcc attgccgttt cgttagtcgg acctgtttca
     7921 tttattgggc tgttggttcc gccagttgtt cgcggaatgg ttggaacgga ctatcgttat
     7981 gttctacctg tctctttttt agttggcggt ggattggtta tgttggcgga ttttacagcg
     8041 aggatgatca atcccccttt tgaaacacct tttggattag tcattgcatt gattggcgtt
     8101 ccgtttttac tgatacaagt aaggagggaa caacggtgaa aaaaatatat tggacgttct
     8161 atttcttgct ttttgtactt gtcatcctgt ctataggtag tttaatgatt ggtacgcctt
     8221 ttatttacct ggatcagctt tttgccacgt tcatagggaa agaatcatct tctttggcgc
     8281 tgattgttag agaatttcga gttcctcgat tactgatcag tttattagca ggagcttgtc
     8341 ttggcgtttc tggttatatc ttgcaaggag taacaagaaa tgaattggca gattctagta
     8401 tattgggcat taatgccgga gcaggatttt tagtgatgct ttatttaggt ttcttttcgc
     8461 actattcgct gccgtttcta ttgctagttg tagcctttct aggtggtgtt ttagctgctt
     8521 gctgtgtcta tcttgcggcc tatgatcgaa atggcttttt gggtatgaat cgaatcttat
     8581 tgtccggtat tgccgttaat gccggccttt ctgcattaac attgctatgc acgatccaat
     8641 tatctaagga aaattatgga tttgtgaatg catggttagc gggatcaatc tggggtgcga
     8701 gctggcctta tgtttgggca ttgcttccat gggcggttat cttaattccg ctaggaggac
     8761 tttatgcaca gaggattgga ttgctatcat ttggtcaaga acgcgcgcaa ggattaggcc
     8821 ttaacgtggc taaaagtcaa aaaatccttt tgttgttagc tgttgctttg gcaagcggta
     8881 gtgtagcggt gacaggtagt ttatcttttg taggactact agctcctcat gctgcgaagc
     8941 tagtcgtacg taaagaaaat aactggacct ttgttctgag cggtttgttc ggttccattt
     9001 ttctgcttag tgcagatatg gttgctagag tgattctacc aagtggggaa gtacctacag
     9061 gaattttgat tgcccttttt
//
LOCUS       DACDHL010000097         9029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDHL010000097 DACDHL010000000
VERSION     DACDHL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.098"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..1458
                     /locus_tag="IV228_002392"
     CDS             4..1458
                     /locus_tag="IV228_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP8718245.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="IV228_002393"
     CDS             1534..2694
                     /locus_tag="IV228_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP8718246.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="IV228_002394"
     CDS             2807..3814
                     /locus_tag="IV228_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8718247.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="IV228_002395"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="IV228_002395"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAP8718248.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="IV228_002396"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="IV228_002396"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP8718249.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="IV228_002397"
     CDS             5793..7388
                     /locus_tag="IV228_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP8718250.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="IV228_002398"
     CDS             7404..7718
                     /locus_tag="IV228_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8718251.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8179..8904
                     /locus_tag="IV228_002399"
     CDS             8179..8904
                     /locus_tag="IV228_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8718252.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
BASE COUNT         2767 a         1642 c         2118 g         2502 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgagactcta agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg
     7981 tttccttttt tctttttgtt tttcttcgtg ttttgaaaat tacttactat ttactatacg
     8041 agtagtttca aataattttg acatcctaat cttcaacaac agaaaaaaat ctaaattata
     8101 aaatttaggg ttgactaaaa tataattttt gtataccata tgatcgtgat aatcattatc
     8161 atttagaagg agaaagttat gttagaagtg aaaaagctta atgtttgtta taacgatttt
     8221 ttagcattga acgagatttc tttaaaaata caagaaggtg ctttaacagg tattattggt
     8281 ccaaatggtg ctggaaaatc tacattgctt aaagcaatgt taaatattat ccctcatcag
     8341 ggagaggtct ccattaataa tgaagatatc aataaaaaac tggtgaagat tgcatatgta
     8401 gagcaaaaag ctgatattga ttttactttt ccaataaaag taaaagaatg tgtatctatg
     8461 ggaacatatg ccggaatgaa agtttttcaa agaattaaaa atgcagaatg gcaaagagtt
     8521 tcaaaggctt tggaaaaggt cgatatggga aaatattcta atcatcaaat aggagaatta
     8581 tctggaggac aatttcaacg tgtactttta gcaagatgtc ttgctcaaaa tgcggatttc
     8641 atatttttag atgaaccatt tgtagggata gacttagtta gtgaacgaat aattatggat
     8701 acgttaaaag aattgaaaca tcagggaaaa acagtgctaa ttgtgcatca tgatttaagc
     8761 aaggtaaaga agtattttga taatattatt cttttgaatc gaaatctaat tgctcacgga
     8821 agtgtagaat ctgtatttaa tgaagagaat ttaaaaaaag cctatggaga tactatattt
     8881 attggagagg ggggaacggc ttagcctctc aatccaataa atatattatc tcaaaagaaa
     8941 tgagttaata ccaaattaaa ataacgatgg ttcatagggt tatccaatca aaaccggaag
     9001 gttaaaaccc taactctaat atacaaggg
//
LOCUS       DACDHL010000098         8697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDHL010000098 DACDHL010000000
VERSION     DACDHL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.099"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(33..1580)
                     /locus_tag="IV228_002400"
     CDS             complement(33..1580)
                     /locus_tag="IV228_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8718253.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(1664..2467)
                     /locus_tag="IV228_002401"
     CDS             complement(1664..2467)
                     /locus_tag="IV228_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8718254.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(2470..3252)
                     /gene="phnE"
                     /locus_tag="IV228_002402"
     CDS             complement(2470..3252)
                     /gene="phnE"
                     /locus_tag="IV228_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP8718255.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGHFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(3249..4028)
                     /gene="phnE"
                     /locus_tag="IV228_002403"
     CDS             complement(3249..4028)
                     /gene="phnE"
                     /locus_tag="IV228_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP8718256.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(4000..4791)
                     /gene="phnC"
                     /locus_tag="IV228_002404"
     CDS             complement(4000..4791)
                     /gene="phnC"
                     /locus_tag="IV228_002404"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP8718257.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(4852..5781)
                     /locus_tag="IV228_002405"
     CDS             complement(4852..5781)
                     /locus_tag="IV228_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8718258.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            5976..6659
                     /locus_tag="IV228_002406"
     CDS             5976..6659
                     /locus_tag="IV228_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAP8718259.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            6820..7863
                     /locus_tag="IV228_002407"
     CDS             6820..7863
                     /locus_tag="IV228_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8718260.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            7927..>8697
                     /locus_tag="IV228_002408"
     CDS             7927..>8697
                     /locus_tag="IV228_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAP8718261.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRNIHD"
BASE COUNT         2643 a         1809 c         1716 g         2529 t
ORIGIN      
        1 gctcacacct ttttgaattt tttatttata tactacaaaa tcacttgaaa attatgattc
       61 acttttgctt cgatgcgtgg gtgaatattc aaatagtcgg caattaattc ggtcatgtcg
      121 atttggattt ctcggacaat tttctccgca ccaaacatcg cataatttcc tccgccgatt
      181 gcccgatatt gatttgtcac cacttccagc atttgttgcg gaagaacggg gtgaccttta
      241 aaacgaagtt cggtaatccg ttgtccaaca ggctgtttca tatcaatagt gtactgaatc
      301 ccttcataca tgtcataatt gtagtactgg ggcttaggct gaataaaggc tggattgaat
      361 acgatttggt tattttctac agctaaataa tttgcggtct gctccaacgc cgctctcaaa
      421 tctgcaccat ttatttgaat cactgccaga gtattcggat aaatatagtt ggtgatgacg
      481 tcccgcatcg tgatcgtctc accaaagcct tttccttcat tattgaataa cgcagttcca
      541 gaaatctctg agccgctggc aaataattgt acacgattaa taaactctac atagggatgt
      601 tcctcaagac gagcctgcat cggatcatta atacgcatat ttccgttcac tttacctaag
      661 ggttgatcca accatttctc tagtttttct tctaccggta cgatcgctgc aagaatttct
      721 ggatcaggct ctgatttttc aactgagtgt aaagcaacct ggtgatctgt aacaaccact
      781 tgcccctctt ttttctcgac ggtaagacga atttcaccga tgaactcgcc acgatagcct
      841 ggttgaacaa cgggaactcc atgaaccgtt tcagcaattt tccggtgctg atgaccggtg
      901 aaaagggcgt caatgccttc aacctgctgc aatagctcgt agccttcatt ctctccagtt
      961 agcgcttctg tgtcctcacc ggttaacaag tctttttcaa atccgccgtg gtaactaacg
     1021 attaccaagt ctgcttgttt ccttaataga ggaacatatt cttttgctgt ttcgacgata
     1081 ctcttaaacg taattcctgc taaggtttct ggcttttccc agtgaggaat gtattgtgtc
     1141 gttaatccca agatagcaat ttttacaccc accttttcga taatcttata ggcgtttcca
     1201 aagtatggtt ggtcattttt gttcaccata ttggctccta atataggcgc ttgataattc
     1261 ttaatcgctt cctcgagata atccacacca tagttaaact catgatttcc caacacttga
     1321 acatcatagc tcatttggtt aatcaagtct gtaagctctt tggctgaacc atattcttta
     1381 gcaagataat aactcaaagg agacccttgg atgaaatcac cattttcgat cgtaattacc
     1441 ggtccatctg cttttgcccg taattccttc agtttcgacg atactttagc cgttcctaag
     1501 gccagatcgg ttctgccgga atagtctgtt ggtgaaacat agccatgcat atcacttgtc
     1561 tctaaaaacg taatgttcat tctgcgtgct cctttagctg actcaaaata gaaaaaccga
     1621 agcgtattca gctgttcggt ctcttttttt gatttataat gccttaatcc ttcatttgaa
     1681 gtgtttttaa aatgttgcct ggaataaagt tttctgttgt ctggcgaaca atccttcctt
     1741 gatcgagatg ataaaggtta tacccatgac gttcttcaaa gcgtacgaag ccatcgcctt
     1801 catcttcacc aacaaaggac atactcgata cagtaaaata ggggatccca ttaatttcat
     1861 cagtaaatgt gtgatgggta tgaccgttta ggatacagcg aatatcgtaa ggtgcgatta
     1921 gcttcaagaa acgttcggta ccaggccatt ttggtatact gctttggtga gacagtagat
     1981 gatgatgggt gaccaagaaa atcggttggt ctttttcttg ctctaaggct tttttcagcc
     2041 attgaaattg ctgatcgtcc actataccat ctgcgtatcc atagcttgaa ttatcaaagg
     2101 aaatcactgt aaactcagga tatttgacta tctggttgta cggggcagaa gaggcggctt
     2161 catcgcacca gcctacccaa aaatgttctt tgatatcgtg attgcctaga gtaacaagaa
     2221 tcgtcgtttc tcctagcgca ttttccagcc aagtcttcaa gtatcgataa tcgtctattt
     2281 cgccatcttc ggttaaatct ccgctgataa tcacaaggtc caagggtgtt tgctgcagga
     2341 gatgatgaat gcattgctcc agagggataa ggggattctg catcgtagcc agcattccct
     2401 tatagccttc ttcacaagcc tcatactctc tgcgaaagtg tatatctgat atatgcaata
     2461 tattcattct tatctcaatc tctttcttat aattccggtt atttgatcta aaataaacac
     2521 ggttacgact acgccaatca aaataatact cactttgctc caagaccgag tttgaatcgc
     2581 gaatatcaat ggagcaccga ttccgccagc gccaactaag cctaaggtag ccgcactacg
     2641 aaccgctatt tcaaaatgat ttaatgctaa agaacaataa atcggcatca aataggggat
     2701 gcgaacaaag aaaatcgttt gccaaaaatt tgctcctacc gcatgcattt cttcaacaag
     2761 tcgctcatcc atcgcttcca tttcttctgt aaaaagtttg ccaagcattc cgatctgatg
     2821 gacgccaatc gccataacac cagcaaagtg gccaggtccg acgactttca caaaaataat
     2881 tgcaaaaact agttcaggaa aagctcgtaa aacattacag ataagcttgc caatttttgt
     2941 tacccatgga tgtgcttgcc agaggttaac cgcactgatc aaggtaatgg gaatcgctaa
     3001 taccgtagcg atcattgtcc ctaaacaggc gattccaatg gttaataata gcaaactaat
     3061 caaatcctcg ccgctgccat catatagaaa ctgccaatca ggctgaccta accctctcaa
     3121 aacgtccatt cccatggaag gagagaaggt gcttaatcca gagtaatcca gagtagcggc
     3181 cgacaaaata acgagaatca ctaaagcaac taatgataaa agggttcttc tgttcatttc
     3241 acgaagcatc ataacaggga cctcctgaca cgttcactta atttatcaac gatcgcgaca
     3301 gttactagga tcgttagaat aatgattgaa actcgttcat aattcaaaag cgcaagtgag
     3361 gaatttagaa taacgccaat accaccagct ccgacatatc caaggacagt agaagcacga
     3421 acattcacct caaacgcgta aaaagcatag ctcgcaaact gactcaaaac ttgtggagcg
     3481 atcgaccaaa tagcgatttg cattttattt gctccaacag ccgcagcggc ttcaatcggg
     3541 ccaaagtcaa tggtttcaat tgcttgaaac attaattgtg acaccatacc aaaagtaaag
     3601 acagcgatag tcaacacgcc agtcgcttca ccaattccaa acacagcaac aaaaagcgcg
     3661 gctaaaagca gggtagggat ggtccgtact aaacctaaaa gaaaacgaaa gatggtagaa
     3721 agaagacggt tttctgtcac tacagtggtc gctaaaaaac taacagggat cgctaaaatt
     3781 aagccaatga ccgttccaag caaagacatt tgaagtgttt tcaccatagg ctcgactaat
     3841 ttaggtaaat agctaaagtc tggatgaatg aagcgtgata aaaagacagt catttgattg
     3901 gagttttgaa aaacctgaga aaaatcagct gacgtgatcc aggcacttcc ttgaaaacaa
     3961 acaattaaga acagaagaat aagcaggtgt ttataccatt taaagtgaac ggtttctttc
     4021 aagctcatcg atttcgcctg ccttttcttc aaagattgca ttgccataaa tttgctctaa
     4081 gcgttcatcg gttaagtcgt ctggacgacc atcaaagaca acctgagcag cttttaacgc
     4141 gagcacacgc gtagaaaact cacgagccag agggactgaa tgcagattga caatgactgt
     4201 tttatccatc gtttgattga tattttttag gtccatcata accttttgag tcgtgattgg
     4261 atctaatgat gcaacgggtt catccgctaa aataatgggg gcttgctgca cgagtgctcg
     4321 cgcaatggat actcgctgtt gttgcccacc actgagctca tcactgcgtg tctgcaattt
     4381 atcagacaaa ccaacttttt ccagtgcttc ctttgtctgc tggtagtctt ccttcgtaaa
     4441 aagacctaaa atacttttcc aagtggagta atagcccaaa cgtccagata gcacattttt
     4501 ctgaaccgta ctacgcttta ccagattgaa gctttgcgag attagaccaa tctggcggcg
     4561 aatctgtcgc agctcttttt tattcgcttt tgtaatagag gttccattaa tgcgaatatc
     4621 tcctttggtg gtttctgcca agcgattgat actgcgcagc agagtgctct ttccagcacc
     4681 actcaaccct atgatggaaa caaattctcc atcttcaatt gttaagtcaa tatccttcaa
     4741 gccaacaacg ccattgggat agaccttcga aacattgtca aaaataatca ttatccttct
     4801 ccattttttt aagtgtaagc aaaaaaaagc ttgctgacac ttactgatgt tttattctgc
     4861 tgcttttttc gtgtagtctc tgatcgtgtc ataggcagaa tcagcagctt cttcatagcc
     4921 ctgatgcccc cacaaagcca ttgcttcttt gccatcttta tcatccttca tatttaagaa
     4981 ggtctcttta attttcgctt ttaaatcttt tgacatgtca ggctgtacag cgattgcgtc
     5041 attcggaata tcgccttttg tcaggtacag gacttttaaa tccttaaata aatcattatc
     5101 tgggaaattc gaagcaaaaa catttcgcgc gccttcaaaa acaaacgccg catccatttg
     5161 cttgttcaac actgcggtaa tctcactcgg aatatcattc acagtggtca tctttgtatc
     5221 tgcagtagga tcaatcccag catctttcaa ttcagcgaca ggatagatgt agccgctagc
     5281 agagttagga cttaaggttg cgatattttt tccttttaaa tcagttaact tattcaatcc
     5341 gctatcggca cgaaccaata cttctccttt aaaggtgttc gtaagtttgt cctttaatgg
     5401 caagccagtt tcttgatcat aatcgccgag ctgagaagtc aggattgcat ctgcagctcc
     5461 agcatccttt gcttgaacat aggctgcagg cggcataatt ccgacatcga cttgaccaga
     5521 agacatcgct tcgacgatcg ttgaataatc agtagctaac gtcacttcta cctcacgatc
     5581 taactttttc gataaatact tagcaaatgg ttttgctttt gcctccatac taccatcatt
     5641 gtttgttgga acgaattgaa caactaattt ttctttgctc tctttttctg agctgctact
     5701 tgtattccca gaaccgcagg ccgccaaagt ggtcaataac atgactccga tgaacccttt
     5761 tgctaactgt ttgaactgca tttttctcct cctgttggat ataatttcta atacgtctaa
     5821 atcatagcag ggaatcaaaa tcaatgaagt gacgcacgtc ctattttaca caacttttac
     5881 atacaattta caaacaatta taaataatta ttcgtattga gccgaaaaac aatctaagct
     5941 ataataaaga aaaacttctt agaggtgacg atttcatgaa aaaaatccat gatacgaagc
     6001 aattaactgc ttacgttgag aagcacaacc tgcaggatta tatggattca gatatattgg
     6061 caatggcttc tttacttttt tttgaaaagg aagagcacct cattcaaacg ggaaaaacct
     6121 cagattattt gtatttttta gtggatggga cggtaatggt gtactcgtat tcttctgata
     6181 cacaaaatat ttgcatcgat tacgcaaagc ccgctacacc actcggcgaa gcctcttcat
     6241 tgtgggaatt atcgccgaaa agcagcgtta aagcagtgac taagtgcatc tgtatcagca
     6301 ttccgctaaa tcaatatcga acacagcttc aaaaagatgt tcgctttttg cagaatattt
     6361 gccagttact aagttatcgt ttaaattccg ggataaattt ggcgaactca ctgacagaac
     6421 ctgttgaaac tcgtttagca aagtttattt tgacccatca cgaaaacgaa cgtttctcat
     6481 ttcggttaac cacctgcgcc tcaatcttaa atgtcagtta tcgacatctt ttacgaacaa
     6541 tcacgaattt tcgtgaggag aatattatcg aaaagaaaaa gaattattac ttgattcatg
     6601 atatcgaagc tttagaggac ttagctgaga atataagtag tccagtaaaa gcagaataac
     6661 agctataaaa aaacagcagt cagaagtgat tgctggtttt ttgatagtgg agaaattggg
     6721 aaacggaaaa tcttcgttga agagtagtgt tctatatatt ggtatgctat acttttaaaa
     6781 gttcaatgtg tcaagacaca taaaaagggg agaaaaattt tgaagaaaat acttgtttca
     6841 ttgttgtttt tggtgtcatt agtaacgtta gtcggctgta gtcagtctca gggggagaag
     6901 caaagtgaaa aagagaaaag taaaatcgct ttggttcttg gatttggcgg ggttgatgac
     6961 cgctccttca atcaagctgg ctgggaagga ttagaggctt ggggaaaaga gaaaaagctg
     7021 acagcaaaaa aagattttac gtatatagaa acgaacaatc cagaggataa cagtaccaat
     7081 atcgatcagg caatcagaaa cggctttacc accattatta gcttgagcta cttgcaagca
     7141 gaggaactgg aaaatgctgc taaagataat ccaaagttga aatttgcctt ggtagatggt
     7201 gagattactg ggaaaaagaa tatctcatca gcaaccttta aagatcaaga aagtgcgtat
     7261 ttagcaggac tagctgccgc atatacgaca aaaagtgatc aagtaggctt tattggtgga
     7321 atggaagggc ccgtgatcca acgtttccaa agcggatttg aagctggcgt taaggcaggg
     7381 gccacagcac tgaacaagga aattactgtt aacgtacaat atgctgcatc ctatgattcg
     7441 ctagataaag gaaaagctat tgctgcttct atgtacaaag agggagtcga tattctttat
     7501 caggcggcag gaagtactgg agctggggtg ttccaagaag caaaagcccg caatgaaaac
     7561 gactatagca gaaaagtatg ggtgattggt gtagatcgcg atcaagcgga tgaaggggat
     7621 tataaaatgg agaacggcga gacagggaat ttcacgttga cttctacaaa gaaaaatgtc
     7681 ggtgcagaag tcaaagaatt tgcagaaact tctcataacg ataaatttcc tggcggcaag
     7741 catttgacct ttggtttgaa agaagaaggg gtagatctga caaaaggaca attatcagag
     7801 gaagcctatc aggcagtttc agacgcaaaa gagaaaatca tcagtggtga tttcagactg
     7861 tagacaaagt ccattttatg gatgatgtct acagtatttt cttttataat agatttatga
     7921 aaacgaatgc aaggagtgat agctatgatg tctaaaaaca atacgaatgg tagaaatcaa
     7981 tttgcgatgc ttacgattga tgatctagtt cctcaagatc atctcgttcg aaagattgat
     8041 gccgcgcttg attttgaatt tatttatcca atcgtcgaag ccacttactc cgatttaggt
     8101 cgcccaagta tcgatccagt gattctgatc aaattggtgt ttattcagta tttattcggt
     8161 atccgttcca tgcgacaaac cattaaggaa gtcgatacca atgtcgctta ccgctggttt
     8221 ttaggctact ccttcgagga gaaaatcccc catttctcaa cttttggaaa gaattatgtt
     8281 cgccgattta gagaaacgac ggtgtttgag gatatttttg cctatatttt agaacaagca
     8341 gttaaagcag gttttgtaac agaggataat ttgtatctgg attctaccca catcaaagct
     8401 aacgccaata aacataagtt tacgaaagaa atgacccatg atgaagcgaa agcctatcag
     8461 gatgaattag aagatgagat caatcgtcaa cgaatagagg ctggaaaacg cccttttact
     8521 ttggacttag aaaaggaagt gaagctgaaa gaaagaaaaa taagtaaagc tgatccggaa
     8581 agcggctact acgtcaaagg cgaacgagaa aaacaatttg cttattctgt ccatacttca
     8641 tgcgacgaca atggatttat tcttagcaca atcatcactc ctagaaatat tcatgac
//
LOCUS       DACDHL010000099         8678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDHL010000099 DACDHL010000000
VERSION     DACDHL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.100"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1172)
                     /locus_tag="IV228_002409"
     CDS             complement(<1..1172)
                     /locus_tag="IV228_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8718262.1"
                     /translation="MDTELLKKMKARREQRLTELREKVESGELREADLEAITKEIDAV
                     VDELNGIKSELSEADRSDEGSDADEGTGGSDGSTGSDENRSGEGDEGDEGEGGDNDDS
                     ENRSGMISQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARAL
                     GIEAGNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKK
                     ERSTDIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEI
                     NYMFNGDDTGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKG
                     RFIINRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDT"
     gene            complement(1185..1760)
                     /locus_tag="IV228_002410"
     CDS             complement(1185..1760)
                     /locus_tag="IV228_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP8718263.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSPTLLWD
                     DLSEVIAPGAFARTISNSDVRCLFNHDWSNVLGRTKSGTLRLSEDDHGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTISEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(1726..2913)
                     /locus_tag="IV228_002411"
     CDS             complement(1726..2913)
                     /locus_tag="IV228_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8718264.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNLKGGDGNEEEGN
                     SHD"
     gene            complement(2977..4704)
                     /locus_tag="IV228_002412"
     CDS             complement(2977..4704)
                     /locus_tag="IV228_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8718265.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(4701..5153)
                     /locus_tag="IV228_002413"
     CDS             complement(4701..5153)
                     /locus_tag="IV228_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP8718266.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWSDKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(5264..5644)
                     /locus_tag="IV228_002414"
     CDS             complement(5264..5644)
                     /locus_tag="IV228_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8718267.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(5641..6042)
                     /locus_tag="IV228_002415"
     CDS             complement(5641..6042)
                     /locus_tag="IV228_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718268.1"
                     /translation="MAMIKIRTSITGTEYWDSEKKKTVVVPKGQEPKFDTSEQTDMTV
                     FAKAIVGDGVLFKSGEEIHLSGEELDSDGNTAADFDGDEATNDQSTEDTDELDNMTAK
                     ELRAYAKKHGIDIPGAVRAKGDIIHLIREAE"
     gene            complement(6023..6409)
                     /locus_tag="IV228_002416"
     CDS             complement(6023..6409)
                     /locus_tag="IV228_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718269.1"
                     /translation="MNDFHEAVLSIEVEPSLATAYKKAIEDDNSRHWIKNEIKDADGN
                     IVISDIKPSWNGNHCSVSIDNSLTIEKYSGAIVDRTERKIQLTIALLSRTLPNLQEQV
                     EWYERMGAKVISTNYKGENQNGNDQN"
     gene            6892..7071
                     /locus_tag="IV228_002417"
     CDS             6892..7071
                     /locus_tag="IV228_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP8718270.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            complement(7064..7477)
                     /locus_tag="IV228_002418"
     CDS             complement(7064..7477)
                     /locus_tag="IV228_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718271.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(7501..7737)
                     /locus_tag="IV228_002419"
     CDS             complement(7501..7737)
                     /locus_tag="IV228_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718272.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLTN"
     gene            complement(7958..8434)
                     /locus_tag="IV228_002420"
     CDS             complement(7958..8434)
                     /locus_tag="IV228_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718273.1"
                     /translation="MRFQWLKDYQELDEQILYLKWNLNKSKLELNRWVNGDLADVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVGQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
BASE COUNT         2387 a         1868 c         1357 g         3066 t
ORIGIN      
        1 gtgtctggtt cacccttttt atctgctgca tctgtccaat ccacaggata gccaactaat
       61 tggtaaccaa ttccaccttc tgcttgtgtg aatggccgca acaaaggaaa tccatcatct
      121 gttttcattt tttcaatggc agtcaaagca gctcgattaa taataaagcg tccctttttc
      181 atcacttctg tcactggtgt atttttaaat tcgattaatg catcatataa tttttgccca
      241 gcacctgcag cagttagatc taaaggtttt tcaaatgcta cagccttttt ggctaatgca
      301 ccaggatttt catttccagt gtcatcacca ttgaacatat aattgatttc tttacgcaca
      361 taagcttttt tcaattcttc cacaacaata tcttcaactg gaacaccaga catttttagt
      421 aattttttag ttactgttgc caaagcatcg aattcggcag gatcaagcaa aatttcatca
      481 aactgaatag ctgtttcagc aatatcagtt gaacgctctt tcttgtttac attcgcatct
      541 gctttcttca caagaattgg atatttgaca tctcctgatg ttcgcaccac tgttccgtat
      601 ttacgaagta aattttcttc ttgagcataa gtaataactt cagatgcaat tacttctggg
      661 acagtaactg aaccgttgcc agcttcaatc cctaaagctc gagcttctgc ttcagaaata
      721 tttccaacta caaaattagc aaatgctttt cgtagttgtt gatctttttg tttattggtc
      781 atttttgcac gtgcctccaa tccgttctta attgatccaa gtaatccgtc tcgttgctgt
      841 tgactaatca ttccagaacg attttctgaa tcatcgttat ccccaccttc cccttcatca
      901 ccttcatcac cttcacctga acgattttca tcgcttccag tcgagccgtc tgatccacct
      961 gttccttcgt cagcatctga accttcatct gatctatcgg cttcacttag ttcacttttg
     1021 attccgttca attcatcaac aacggcatca atttcttttg taatagcttc taaatcagct
     1081 tcgcgtaatt ctcctgattc aactttttca cgtaattcag tcaatcgttg ttcacgacgt
     1141 gccttcattt ttttcaataa ttctgtatcc atgtattttt cctcctacgc ttctagcgct
     1201 tgattaattt ttttgattaa tttttttctt tcttcgacgt ttttttctaa ttcatcacga
     1261 cttcttaatg ctgcctctgt atcttcataa gccggcaaag gaacaataga aacctcatat
     1321 aattccactt cgctaattgt tcgaagcata ggttctgaat tgtagtccca tgtttcttca
     1381 gttggtacaa atccaaaact acattgatta atgtctccgc gttccataga tttaaccaag
     1441 tctcttgcta ccgttgtgtc tggcaaatcg acttcgaatt tcaaaccatg atcgtcttct
     1501 gacaatcgaa gggttccgct tttggttcgc cctagtacat tagaccaatc gtggttaaat
     1561 aagcaacgta catcggagtt actaatcgtt ctagcaaaag cgcctggtgc aatgacttca
     1621 ctcaaatcgt cccataatag tgttgggcta ttgaatacag cagcatatcc actaatggtc
     1681 ctcgtatgag tttcttcatc agaacgcgtt gaaaggttgg tgatgtcaat cgtgcgaatt
     1741 tccttcttct tcatttccat cacctccttt caagttttga tcattcgttg gtaaggaatc
     1801 atctgttgca tttttctggc caatcctaga caagtcattt gaaatataga tagcttgtgt
     1861 ttctggagtg ttctgtttag gaaaaccaag catttctgcc acattatctg gacttgtaat
     1921 cccagtacga acaatgttgt agccaatatt tgtttttgtt gagtaaggaa caaaatccaa
     1981 aatgttaatt ttccattcca ctcgatagcc agaattaggc ataaaaaaaa gagcggtgta
     2041 atgttcgctc ttgttcttca atattggttt gattgctttg ttgtgcagat acatcatcgc
     2101 tttttcgatg tctgttttca tcaacgattg atacgtatca acattgattc ctaaaaattt
     2161 acctaagtct tttttataaa cacctaaata gttcaaaata gccgcgtcat caacaggact
     2221 ttttaacgtc tcgatggaat atccttttcc cagaggaatc atcttaacag aatgatcact
     2281 gtcattttgc gttccttcca gttgatccaa tatagctttg acaattttcg tttgggcgct
     2341 attatttgga ttgatgtggg cgtccagttt aagcatgaac gcgagtaaac cacctttagt
     2401 atatttgtcc gtcaaaactt tttcagcgct cagaacgcct tccagagtgt ttcttgcaag
     2461 gtcaattatt ccagcacctt ttaatgaatc agttccgatg ttcttaatat gtcgaatcat
     2521 ttgaccaggt attttttgtc cattcatttc aaattcttct tgaagtcgtt cattgatttt
     2581 agtggttaca ccgtatgcca aatgaagctg gtcccgatct gttaatggga atgtctcacc
     2641 attgatcagt agggtatttg tttccaattt ggtaaattcg aatccggtca aataatcatt
     2701 gggattcttc aaaattttta gcaagtggtg gtccttcact tcattaccgt ctggacctat
     2761 gacaacaggt gaggacaacg ctacctggtt tgagatatcc tggaccaatt cataaacatc
     2821 agaagattcc atgatagagg aatctgttac atatctttga ccgtaacgcg tatagtggcc
     2881 aaacatatcc tcgatgtacc cacgcttttc cataaaggaa tagactgcat tcgataaccg
     2941 atcacgtaat ttcaatattt ctcaccgcct ttctatttat ctatagatgg aactaaggta
     3001 atcatctaat tcatccgaat caatatctgt catctgattc atcgtttcct tatgaccaca
     3061 caaaaacgcc acgaaaccat caatcttttt ctttgattga cgtttacttg gcgctttttg
     3121 tccgttgatg ttagtgattg ctacaacgtt caaggtgcaa taaaggaaca atggattatc
     3181 aaattgaatt cgtttctcat aaaacaaccg ttcgacatca tcaaaaggag cgttcaacac
     3241 tttaggatat tgggcaacct caacgcattc caatcctaag ttctccaatt tctcaacaag
     3301 tttgtcgctc atcgctggat cataattcac ttgttgtata tcgtataaat ccatgcagtc
     3361 ttcgatgtac tgcaagattt gatcttgatc aatcatttta ccatcgcaaa attcaacaaa
     3421 accttgttca gctaaatcgc tgtaaggcac gttatcttct ttttctcgaa actctaaatc
     3481 ttcattggga ataaaataaa gctgcttcac tttaaggacc gcttttccat cttcatccca
     3541 cgttgggaag tttaaagaca cacaggtcaa atctcgtgtg cgtgataaat ccaaaccaat
     3601 gtaacaaggt tcaccactta aatttccaag ctcttgggta gtaaccaaac acggctctac
     3661 ttgatcctgt tcaaagaaat tatccgcacc gtttacaaat acatccaagt gcttcgttag
     3721 aaactcggct tttgaatgtg ctgaacgttg tgccgtttta aacgctgact ccaaggcgga
     3781 aagatcaact gatattcccc agttaggatt gcacatttcc caaacttttc tatccgtcca
     3841 atcatagttt ttatttggtt catagattag aacaaagttc gaatcattat catcacgttt
     3901 caacacttct tttgcttcac gatatacgcg cattccaaca gacgatgaac ctttcccagc
     3961 agttgaaata ttaaacatca atggttgtgg caaagagatt tgtgcagact taaaattgtc
     4021 atactgttcc attttctctt gcttatgcag ctcatcattc aatacaaaat atggattaga
     4081 accctcaatg ttatcgatat tcttcgtttg aacaataaat ttattcgaat aagccatatc
     4141 ttcatgaata taatcataag taatactaga aacagttccc tttggtcctt tgaatatttt
     4201 agttccttca agtaggattg gattgtttaa tattgtagct gcaaaaggtt tggcagcata
     4261 ttgagcttga gcaaagtcag aagcgcatgc ataacaatcg actgacaaag caccttctcc
     4321 atacattgca tagcctaatg aaccgacagc aatcaatgtt ttaccgttct ttttagggat
     4381 ttggacatac gcttcacgag taacacggac aatttgccct ttttcatttt ctttaaccca
     4441 tccatacatc cacgagtaaa taaatttttc ccaaggttct aaaatgaatg gctttccaac
     4501 catctcacct tttgtgtgaa caataaacga ctcaacccag tccatcattt cattcgcacg
     4561 atctacatca aaccaaatat ctttacgttt tttccaccga taccaacgat ccactgccaa
     4621 acgaacagtt ttaggatatt tcccaggttt ctttcttact tcttttgcaa ataaatcggc
     4681 ataatttaca ccaggttcga tcatttttca gtacctgcct tcttacgcca tttgtttctg
     4741 tgcgctgcta gttcatctac tggcttttct tctggacgtg taatttcttc atcttttctt
     4801 gcagtcgatc cgccagttat ttgtctacca gttttagcct tattcgttag ccccaacaaa
     4861 tctagagctt tcattttctt atctgaccaa gtttctactt gctgcgccaa tggatgcttc
     4921 gattgattag tggcacctga tttattcgtg aatttttgcg tctccggaaa gcctttttcc
     4981 ttccacaaaa gatatttgtg ttggtaaatt tcaaaaatat ccaaatatga ttcgattaat
     5041 ggatcaagag tgatggtgta caaatcagac aaattcatta tttttaaaat acgagctttt
     5101 tcagcactta ctttttcatc aacaatcgct ttacgttgcg ctttagtcgt catacttgta
     5161 tacacccccc tttgtttttt gaaaaatttg acctaacgat gcgcgtgact ccccgctacc
     5221 ctatctcccc acgcgaaaaa attttgaaaa tggatagggg gggttaatta aaatacgaag
     5281 gaaaaacttt tttgtcttct gtttcgtttt caacaattgg atgacatttt gaacataaaa
     5341 gcatgagatt gtttggatca agcttaagca gttcattatc tttgattggt acaatgtgat
     5401 ggacgtgtgc cctctttcca aagatgaact gaccacatcg ctgacagtgg ccaccttctc
     5461 tttcgtaaat aaattggcgc atatctttcc atgcttgcgt tcgatagaat ggtttgttct
     5521 catgatgata aacagacttt gcttgctgct tcttcttgcg tgatctgctt gatctcttgt
     5581 gttcagtaca gtagataccc tttgctatct tgttcgtgca tccgtcaaac tgacagtact
     5641 tcattctgct tcacgaataa gatggatgat gtctcctttt gcacggacag caccaggaat
     5701 atcaatgcca tgtttcttag catatgcacg caattctttt gcagtcatat tgtccagttc
     5761 atccgtatct tcagtggact gatcattagt agcttcgtct ccatcaaaat cagcagcggt
     5821 gtttccatcg ctatctaatt cttctcctga taaatgaatt tcttcaccac tcttgaacaa
     5881 gacaccatca ccaacgattg ccttggcaaa tacagtcata tcagtctgtt cacttgtatc
     5941 aaatttaggt tcttgacctt tcggaactac aacagtcttt ttcttttctg aatcccaata
     6001 ctccgttcct gtgatggatg ttctaatttt gatcattgcc attttgattc tctcctttgt
     6061 aatttgtact gattactttt gcacccattc gctcatacca ttcaacctgc tcttgtaagt
     6121 ttggcagtgt tctagaaagc aatgctatgg tcagctgaat tttccgttct gttcgatcaa
     6181 ctattgctcc agaatatttt tcaatagtca ggctattatc aatactgaca ctacagtggt
     6241 tgccattcca gcttggcttg atatcgctaa tcacaatatt tccatcggca tcttttattt
     6301 catttttaat ccaatgccta ctgttgtcat cttcaatcgc ttttttatat gcagtggcca
     6361 gacttggttc tacctcgatg ctaagtaccg cttcatgaaa atcattcata gataaaactc
     6421 ctttcaaaat aaaaagacca ctcaatgagt gatctaatat gtaatacagg acccactatc
     6481 cttcgaggga atgtggatta cagaaacatc agcgcgctat agtttccaac ctgtatattc
     6541 ggttttactg acaggcagtt atggatcacc gtaaaccaaa gtcactggca atgaatcgaa
     6601 cattgcaaca ccattttccg atattttaca gactctccat cataaaaact ctcacctcta
     6661 gaattgtcta tctttagggg atttatttca agatgcctct tgtcctttac cctcgacggt
     6721 ctcttgccta gctgcataaa ctaagctcag gtgctcatta ccactgattt aagttattag
     6781 cgttaccttt ccgccacagt gacaaattta tattgtgaaa ataaatacta agcgtataat
     6841 tttaattatc agcgagtggt ccgctgaaat aaattatagg tggtgaaaaa tttgtacttt
     6901 gttataagag aagcaactaa tggccaatat tacttcgtta ttaagtccaa taacaatgaa
     6961 gtagtagcaa ctagcgaaac atacttgact aaatattccg ctgaacaaac cattaattct
     7021 ataaaaaacg gaatcaccaa agactctcaa gttatcgata tgactaaata agacgactaa
     7081 cttcatttgc tagggagttc atttcagctg ctttttctac caaagcagtt gagatacttt
     7141 ctagcttttc tgatttaaca gaattaaata ctggcgtctt gatactaatc gatacatctt
     7201 gacgaggatc aggactcgtt gctctatttt tttgcaccac tgatctgtac ttttctactg
     7261 aatgatactc aattacttgt tctaaatgtt ttttgtactc tttacataca gcttcttttt
     7321 ggatcaattc taaacgttgt tgctcaataa tatcaatcaa ccgttttcga tccatacctt
     7381 gatactttat ttgttcgact tggtcactag ccttgttgag cagttctttt agttcagtca
     7441 aatctaattt gacagttgct ttgatttctt tatccattcg ttttccctcc aatacataaa
     7501 ttaattagtt aattgttgct cattgatctg gagagctatt tcttcttcta taagctcaac
     7561 gatcttcctt tttgtcgaat ttaattcgaa cttatgatcc attaaagtaa gctttatgtt
     7621 cttaatcttt tcaccttcga attctttgat aatatcttcg attctttggt ttactaaaat
     7681 agttagttga tcaatttttt cggaagcaat cttcatttta tataccctcc aatacataaa
     7741 ttaatagaca gcagcagttg aaagacgaca agaacgtgtt tagttttttg tgcgctgctg
     7801 tctaatcaaa ggcaatagaa aagagccaca gataccaatt aataaaacag ttgggatatc
     7861 ttggctcttt catatttttt tgacactaat agaatatcat gctgctaaca atgtttacat
     7921 agttacaata ttcgaacatt tagtgaacat tgtaatctta gaataaagag agttgttctt
     7981 tgtacttctc gctattgtaa aaatcaattt cgttttcttt tttcaatcgg tcagcttgtc
     8041 tgtgttcata ctcatctaaa aagttcaatg tctttctaat ttctgcatgt cgctgtctga
     8101 catacgatga gctataccca acagattcag caatatcttc taagctcatt tgaccaacat
     8161 atttcatctt aacaatttga ttatcaatac cagaaaagct atctatcagc aataacattt
     8221 cttctttttg ctcgatcaac aattccaatt cattttctat cttttgaata ttctcttcta
     8281 aagaagaagt tctcgaattc ttttcgattc ggacgtctgc taaatctcca ttgacccatc
     8341 gattcaattc aagcttactt ttattaagat tccactttaa gtaaagaatc tgctcatcaa
     8401 gttcttggta atcttttaac cactgaaatc tcacaaacgc caccccttat atgataaaat
     8461 agtattgcgc tgctatctcg aaagaggtgg ctttttttat tttttcttag ccgttccatt
     8521 ctggattgcc ttttgttcca aacgacgttt tttcttcttg attttcgatt ttgtttttcc
     8581 catacaaaca tcctttctgg aatatatatc gttcgatttt tactgtttaa caagagatgg
     8641 ccaaatttta ttgttcgctt tgaatcatct ctttgaaa
//
LOCUS       DACDHL010000100         8483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDHL010000100 DACDHL010000000
VERSION     DACDHL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.101"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            111..1742
                     /locus_tag="IV228_002421"
     CDS             111..1742
                     /locus_tag="IV228_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8718274.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1745..2824
                     /gene="uxuA"
                     /locus_tag="IV228_002422"
     CDS             1745..2824
                     /gene="uxuA"
                     /locus_tag="IV228_002422"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8718275.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2898..4325
                     /gene="uxaC"
                     /locus_tag="IV228_002423"
     CDS             2898..4325
                     /gene="uxaC"
                     /locus_tag="IV228_002423"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8718276.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4409..5056
                     /gene="eda"
                     /locus_tag="IV228_002424"
     CDS             4409..5056
                     /gene="eda"
                     /locus_tag="IV228_002424"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8718277.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5176..6552
                     /locus_tag="IV228_002425"
     CDS             5176..6552
                     /locus_tag="IV228_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8718278.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVNWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            6562..7356
                     /locus_tag="IV228_002426"
     CDS             6562..7356
                     /locus_tag="IV228_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8718279.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            7369..>8483
                     /locus_tag="IV228_002427"
     CDS             7369..>8483
                     /locus_tag="IV228_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8718280.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIRMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEA"
BASE COUNT         2931 a         1104 c         1740 g         2708 t
ORIGIN      
        1 atatggtaga aaaaagcaag tttattccct ttttatattg acaacggtta caatgggttt
       61 taaaatataa aatgtatcaa aactaacata tcagtttgag gaggttaatt atggtttcga
      121 ttaaaaattg gcaaaattct atcgaagaat tcaatgaaat gcagattcta ctgccgtgtt
      181 ttgatgtcaa agaagttaag caagaaggcc agcaacatcc agtatggctt cattttggtg
      241 gaggaaatct ttatcgagga tttcatgcag aaattgctca atgcttagca gatcaaaatg
      301 aattagaaac aggagtagtt gtgtgtgaaa catttgatga agaagtagtg gaaaaagctt
      361 atcatgacta taacaatgac attttggaaa ttgttatgag tggagatggt gaactcaaaa
      421 aaagactttt ggcagtaacg gctgatagtt tatattgcaa tccgaagaat atagcggact
      481 atcaaagagt ggttaaatat tttgaagatc ctaaattaca gtttacaacg tttactatta
      541 ctgaaaaagg ttacagttta aaagattcta aagggcaatt cttttcggta gtcaaagctg
      601 atatcaaatc tggacctagc aatcctcaac atacgatgag tattgtaaca tccttgttat
      661 atagcagatt taatgctgga gagttgccga ttgcgatggt ttctactgat aatttctcac
      721 agaatggtca acgttttcaa gatagtattt ttacgattgc agaagcttgg caaaaagaag
      781 gatatgtttc tgaaaaattt attgcttatt taaaggataa agaaaaagtg acttttcctt
      841 ggagtatgat tgatcgaatt acgccaaatc cttcagagaa tgtaaaggaa gatctattga
      901 tgatggggct cgaggattta gatatcattc acacaaaaaa acataccaat attgctgctt
      961 tcactaatac tgaggtagtt cattatttag ttattgaaga tagttttcca aatggccggc
     1021 ctaaattgga aaaggctggt gtgattatga cagatagaga gacggttgat aaagcagaca
     1081 tcatgaaggt tacgacaagc ttgaatccac ttcatactgc tttggcagta acaggttgtc
     1141 tgctaggata tcaatcaatt tcagcagaga tgcaggatga tgatttggtt ggtttaatca
     1201 aaggaatagg ttatttagaa ggtttaccag tagttgaaaa tccaggcatt attgatccaa
     1261 aacaatttat tgatgaagta attaataaac gtttgcctaa tccctatatt ccagatacac
     1321 cacaacgaat tgcaacagat acttctcaaa aagtagcgat tcgctatggt gaaacaatta
     1381 aaaagtatgt cgaaaaagac ggaaatgcag aaaatctaac atttattcct atcgctattg
     1441 ctgcttggtt acgttattta ctggcagtcg atgatcaagg ggaaaatttt gtgccaagtc
     1501 ctgatccgtt attggtagag ttgcaggata aattatcagt attgaaatta ggtgaacaag
     1561 atccttcaaa gattcattgt gcggtacagg aaattttaga aaataaagaa atctttggtt
     1621 tagatttata tcaagttggg gtaggaaaga aaactgaaaa aatcttttgc gaaatgctag
     1681 taggtcctca agcagttcgt aaaacaatca ataaatatat aacagaagtt ggaggaaagt
     1741 aactatggaa atgactttta gatggtatgg ggcaaatgaa gaaaaaatct cactacaaga
     1801 aattcgtcag atcccagcaa tgaaaggaat tgttggaaca ttaatggata taccagccgg
     1861 agaggtttgg ccgagagaac gaattaaatc tttaaaagat gaagtcgaag ccaatggact
     1921 taccttgaaa gttattgaga gtgttaatgt tcatgatgat atcaaaattg gtttaccaac
     1981 acgagatcaa tacattgaga attataaaca gacgattcgc aatttggcag aatttgggat
     2041 tgaggttatc tgttataatt ttatgccagt atttgattgg gtaaaatcgg atttggatta
     2101 tcgtttagat gatgggtctt ctacgttagc ctatatttca aaaaatatcc ctactgatcc
     2161 ggaggaaatt attagagaag tagaaaattc tactaataat tttgaattgc cgggttggga
     2221 acctcatcgg ttatcacaaa ttaagcaatt attagaagcc tataaagaag ttgatgaaga
     2281 gaaattacga gagaactttg catattttct caaagcaatt atcccgacat gtgaagaagt
     2341 tgggattaaa atggcagttc acccagatga tccaccatat tccattttcg gattacctcg
     2401 tattgttaaa aatcgtgacg atttagattg gttgtgtaat gtagtggatt ctccttctaa
     2461 tggtattaca ttatgtactg gaagtattgc agaacgacca gataatgatg tctatgaaat
     2521 tctatctgaa ttttgtcaac gggatcggat tccttttgca catgttcgta atatcaaata
     2581 tttaagtgac aatggactcg atttttacga agcgccgcat aaatcagaat atggttctct
     2641 agatatgtat aagatattaa aaacaatgta cgataatgga tttaatggct atattcgtcc
     2701 agatcatggt cggatgattt ggggagaaac cggtagacct ggttatggat tatatgaccg
     2761 agctctaggt gcagtttatt taaatggtat ttgggaagcg cttgaaaagg gggcgagtaa
     2821 ataattatat aagggagttt atttagagga aaaattttct cttctaaaaa ctaacggaag
     2881 gaaaggagtg tttaaatatg tttttagata agaacttttt attagagaat gaaatagcta
     2941 aaagactttt tcataaattt gcaaaacacc aaccaattat cgactatcat tgtcatttag
     3001 acccagcata tttgctagaa aataagcaat acaagaattt gactcaggta tggttaaatg
     3061 ataaaggagc tggagatcat tataaatggc gattgatgcg agctaatggt atagaagaag
     3121 agttgatcag tggtccaggt gatgattatg aaaaattttt agcttttgta aaaacagtgg
     3181 agaaggcgcc aggtaatcct atttttgaat ggtctcattt ggaattacaa cgttactttg
     3241 atattgattt gacaatttct gagaagaatg caccagaaat ttggcgacgt gctaacgaaa
     3301 aaattcaaac ggattcctat cgcccgaaag aattgttgga gatgatggat gtgaaagcca
     3361 tctgtacaac ggatgatcct acagatgact tgtggtatca tactgagctt tctaaacagg
     3421 ataattcttt caaggtatta cctactttcc gtccagataa ggtctggagt atcaaatcat
     3481 caggcttttc gaactatatt ttaaaactta gtcaaacagc ggctatacca atcaaagact
     3541 ttaatgggtt gaagcaagca ttgagccaac gagtggatca ttttcatcaa attggctgtc
     3601 gattagcaga tcacggtttg aatacttatt tttatgaaat agcagatgaa aaaatactcg
     3661 acaatctgtt aataaaagct ttagcaggaa aaacggattt ccatttatca gaaattgcac
     3721 aatttgttac aggtattcaa ttacatttga tgtctgagta tacaagatat ggttggacga
     3781 tgcaaatgca tttaaatgct ttaagaaata attcaagtat attaaaggaa caaattggag
     3841 tagatgtcgg tggtgattcg atgggggatc aagctagttt gacacaaaat gttgtagcat
     3901 tgttcgatac cgctgaaaag agtgggtgct taccaaaaac aattttatat tcattaaatc
     3961 caactgattg gcttccacta gcaacagcaa tgcaaagctt ccaagggggt atgattcaaa
     4021 aaatccaatt gggatgtgct tggtggttca atgatacagc tgaaggaatg cgtcaacagt
     4081 tgactacgat ggcacaacaa agtttgttgt cgaattttac aggtatgttg actgattctc
     4141 gaagttttct aagctatcct cgccatgagt attttcgtcg agtattatgt caattactaa
     4201 gcgagtgggt taaagcaggc cgaattccag atgatgaaat gtatcttagt cagatagtgg
     4261 agaatatttg ttatcgtaat gcgtataatt attttaattt ttttgaaatc ggaagtagaa
     4321 aataatactt gaaataaaag cttaaattta ttctttgtaa gttagtacag ctagacaaag
     4381 tatgtatcca taaaggaagg agatagatat gaaaaaagta gaaattttaa gtaagttaga
     4441 agcggctgga gtcattgcgg ttgtacgtgg gaaaaataag gatgaggcca ttaaagcaat
     4501 aaattcaatt attgcgggtg gaattaaagg gattgaacta acttttacag ttcctaatgc
     4561 tgaagaaata atcaaagaaa ttgctaaaaa atatgctgat caaaaagatg taataatcgg
     4621 agccggtacg gttttagatg cgtttactgc tcgtatcgca attatggctg gggcagaata
     4681 tatcgttagt ccatcttttg atcgacaaac agctgagatt tgtaatttat atcaaattcc
     4741 ttatttacca ggttgtatga caattactga gataaaggag gcacttactg ctggcgtaga
     4801 tatcattaaa ctattcccag gttctgcttt tggtccatcc attgtaaaag cctatcaagc
     4861 tccattacca cacgtaaata taatgcctac tggcggagta agtatagaga acttagaaga
     4921 ttggtttgaa gtaggagttg ttgcagttgg tgtaggtggg aatttattgg cttctgttga
     4981 aaaaggtgat tttgaagaag taagtcgagt agctagtaaa tatatagata agttttatga
     5041 gataaagaat tattaggaga aaaaacaaat aatactctga aatgtaaaaa ataaatactg
     5101 actctttacg cttcagagta tagtacgtac tttcatcaga atgaaaacgc ttaacaacag
     5161 aagggagaga attgtatgga aagaaaattt ggtataagag acaaaatagg atatatgttt
     5221 ggagattttg gaaacgattt tactttcatt tttgctagtt cttttttaat gatcttttat
     5281 acaaaagtat tggggataag cggagctgcc gttggaacat tatttttagt agcaagattt
     5341 atcgatgcag ttactgatgt aactatgggg cgtattgttg atagatcgaa agcaactaag
     5401 cagggtaagt ttaaaccgtg gattttaaga atgagtggtc cagtcgcttt agcaagtttt
     5461 ttaatgtatc aaacttctat gatttcagct tctatgagcg ttaaggtagt atacatgtat
     5521 gttacgtata tattatgggg gagtattttt tacacatcta taaatattcc ttatggatca
     5581 atggcatcag ctattacttc tattacagaa gaaagaacaa tgctatcgac attccgaaat
     5641 gttggagcta ctttagcaag tttggtcatt ggtgtattaa caccattatt tatttataca
     5701 agagatgatt caggcgctca aatagttcgt ggtggatcaa cttttacaat tgttgcggga
     5761 atattttcga tatgtgcact tatttgttat ttaatatgtt ataaactaac tactgaacga
     5821 gtaagaattg accaatctca atacaaagtt tcattgttaa aatctttaaa agggttagtt
     5881 aaaaataggg cattactttc tataattggt gcggctattt tcttactcct tgctcaatta
     5941 ttaattatga gtatgaataa ttatgtattt cctgattact ataatgatgc acagggaata
     6001 gttttaatga attttatcaa tcctattttg gttttaatag ttgtttcgcc tttaactttg
     6061 tttttaagta aaaaatatgg aaaaaaagaa ctggcatcag taggaatgtt cttttcagca
     6121 atagtatatt tgatcttatt tttgataaaa gctagtaata tgtatgtatt cttaattctt
     6181 agttcggttg gctatatggg tttgggtgtg tttaatactg ttaattgggc aaatattact
     6241 gatgttattg atgatcaaga agtaaagtct gatcaaagag aagatgggac cgtatatgcg
     6301 gtatattctt ttgctagaaa aataggtcaa gctctagctg gaggcgtcgg aggttggact
     6361 ctttctatta taggctatga ctctttagca aatgtacaaa cgggagatgt tttagaaaag
     6421 ttatataacg cttcaacatt aattccatca atttgttttc tgattgtagg attaatttta
     6481 tttttccttt atccattatc taaagataaa gtcttagaaa attgcgagct tttaaaacaa
     6541 aaacataaat aggagagaaa tatgaataat tggatatttt gtattgattc agatggctgt
     6601 gttatggata cgatgactta taaacacaat ctctttttcg gaccaattgc tgcagagatt
     6661 tttgatgttc atcatagaga aaaattccta caggattgga ataatattaa tttatattca
     6721 gagactcgtg gaataaacag atttttaggt ttagttaaaa cattagaaaa aaatgatgta
     6781 acatcgatta atcgattaaa gcactgggta tctctaacaa aagaattgtc taatccatct
     6841 ttgaagaggg aaatagaata ttctcagtca gaggatttat taaaagcttt aaaatggtcg
     6901 gaagaagtca atcaacagat tagtcaagca aaaggattag ataaaccctt tttaagtgct
     6961 agagatacta taaaagctct gaaaaaatat gggaagataa ttattgtaag ctcagctaat
     7021 aaggaagctg ttcaagaaga atgggaaaga catgaattat tatcattagt tgatgaactg
     7081 tgttgtcaag ataaaggaaa aaaagaagat attattcgaa gtgtaattga aaatggttgt
     7141 gatttggata agatattaat gattggtgat tctcctggtg atctagaagc tgcaaataaa
     7201 aataaagttt tcttttatcc tattttggtc aataaagaaa aagaatcatg ggaaaactta
     7261 agaactgatg ttttatttaa atgtttatcg ggagattatg tagatattga acaaaaatat
     7321 atagatactt tttggaaaaa tcttactaat aagtagagga gaaagaaaat gactataaaa
     7381 gtagatttac gaaaaaaacc atataattta gatgaagatg ctatttcttg gattgaagag
     7441 acgattgatt ctatgacttt ggaagaaaaa ataggtcaat tgtttgttaa tatgggatct
     7501 agtcgtgata aaaattactt gagtaatatg gtcaatcagt accatattgg agctgtacga
     7561 tataatccag gaactgctga tgaggtatat gatcaaaact atatcttaca aacagaaagt
     7621 aaaattcctc tattaattgc tgctaatacg gaagcaggtg gtaacggagc ttgtacagat
     7681 ggtactgaag ttggtgtaga agtaaaaatt ggtgcaacaa atgatgcgaa atatgcttat
     7741 gaattaggga gagtttctgg gattgaagca tcggctattg gatgtaactg gagttttgct
     7801 cctattgtag atattaatag aaattggaga aatcctatca tttctagtcg atcttttgga
     7861 caaaaccctg ataaagtttt agaacttagt ttagcttata tgaaaggtat tcaggagagt
     7921 ggaattttgc ctgcagctaa acatttccct ggtgacggta ttgacgaacg agatcagcat
     7981 ctatctttta gtattaatag tttaggtacg gaagaatggg ataattcttt tggaaaagta
     8041 tactcaggct taatagaagc tggattacct tcaattatgg caggtcatat ccgtatgcca
     8101 gcttattcaa aacattttaa tcctgatctg aaagatgaag atttacttcc tgcaacttta
     8161 tcaaaagaat taatcactga cttacttaga ggaaaactcg gatttaatgg attagtagtt
     8221 actgatgcta gtcatatgtt aggaatgact ggggcaatga aacgatcaga attattgccc
     8281 acttctattg cagctggatg tgatctattt ctatttttta atgatccaga tgaagacttt
     8341 aattatatga tggaaggcta tcgtaaagga attatcactg atgatcggtt attagatgcc
     8401 ttaaaaagaa ttttagggtt aaaagctaaa ttgggattac acaaaaaagc aaaagaacaa
     8461 attttattac ctaagcaaga agc
//
LOCUS       DACDHL010000101         8459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDHL010000101 DACDHL010000000
VERSION     DACDHL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.102"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..604
                     /locus_tag="IV228_002428"
     CDS             5..604
                     /locus_tag="IV228_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8718281.1"
                     /translation="MTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSSVNMQPWRFVV
                     AESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAYESGKMPKEVR
                     DQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGVEADQLAE
                     AFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            958..2436
                     /gene="msr"
                     /locus_tag="IV228_002429"
     CDS             958..2436
                     /gene="msr"
                     /locus_tag="IV228_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8718282.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2483..2929
                     /locus_tag="IV228_002430"
     CDS             2483..2929
                     /locus_tag="IV228_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8718283.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3128..3643)
                     /locus_tag="IV228_002431"
     CDS             complement(3128..3643)
                     /locus_tag="IV228_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8718284.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3760..4962
                     /locus_tag="IV228_002432"
     CDS             3760..4962
                     /locus_tag="IV228_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8718285.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5335..5661
                     /locus_tag="IV228_002433"
     CDS             5335..5661
                     /locus_tag="IV228_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718286.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(5778..6122)
                     /locus_tag="IV228_002434"
     CDS             complement(5778..6122)
                     /locus_tag="IV228_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718287.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(6198..7445)
                     /locus_tag="IV228_002435"
     CDS             complement(6198..7445)
                     /locus_tag="IV228_002435"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8718288.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(7442..8248)
                     /gene="proB"
                     /locus_tag="IV228_002436"
     CDS             complement(7442..8248)
                     /gene="proB"
                     /locus_tag="IV228_002436"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8718289.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT         2531 a         1522 c         1774 g         2632 t
ORIGIN      
        1 aattatgact ggtagaagat ccgttcgcgt atatgatgaa acatacaaaa tcccacacga
       61 agaaatgctg gaaatgatca aagaagcgac gactgcacct tcttctgtca atatgcaacc
      121 atggcgcttt gtggtcgctg aaagcgagga agcaaaagaa atcttgcgtc ctttgatccg
      181 tttcaatacg agacaaaatg atacttcttc tgcaatggtc atgatttttg gcgatatgca
      241 atgttacgaa tatggcgaag aaatctataa ccaagcttac gaatcaggga agatgccaaa
      301 agaagtcaga gatcaacaat tagctgcgat cattccttat tacaagagtt tttcaagaga
      361 agaaatgaac gatgtagtca aagtcgattc tagcttagcg gctatgcaat tcatgttagt
      421 tgcgcgtgat catggctatg aaacaaatcc aattggtgga gtcgaagcag atcagttggc
      481 agaggcattt ggtttggata aagaccgtta tgttccagtt attattttat caataggaaa
      541 agcggtagaa gaaggttacg agtctgttcg tttagcacca gaaaagatca ctacgttcga
      601 ataaaaggtt ttcagttact attcagtagc tattgctttt tttgaatcgt gtgtggtaac
      661 ctaataaaaa aggaggtcgg aaaaatgact gcatcgatga aattacgttt cgaacttttg
      721 aataacaact aaataaattc aaaagacatg tataaacaac aatttcatgg atggagtatg
      781 tccatttttt cgaacgacag tagaataggt gaaagctatg acagatttgg tgatttagtc
      841 atagttattt cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa acagattttt
      901 ctgctctttt ttagtatcga taattgttgt tttacatgaa ataggagtgt aaaacatatg
      961 gaaaatttag cagtaaatat aacaaatcta caagtcagct ttggcaacca gctagaacta
     1021 tctattgatt ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg agaaaatggt
     1081 gtcggtaaat cgactttgct caaactaata gccggtgaac tttttcccga tcatgggaaa
     1141 atccaaacag agatcacctt caactacctg cctcaattaa cctatctcgc tgaagcaaag
     1201 gacctaaatt tggaattagc cagtcatttc cagttaagac tggaagaaac ttcagagcgg
     1261 aaatggagcg gaggggaaga acgaaagatc gagttgatac gtcttctttc ttcttatgaa
     1321 caagggatgc ttctagacga gccaacaacc catctagata gaaaaagtat tgatcgactg
     1381 attgaagagc ttcgttatta ttatggtacg ctggtttttg ttagtcatga tcgctatttt
     1441 ctagatgagc tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg agagttttcg
     1501 gggaattata gtgcctatct cactcaaaag gaattggaga aaaagactca gctacgagaa
     1561 gcagagtcga tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga aaagaaaaaa
     1621 caagcggaaa agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat cagaccggat
     1681 cggttgtctt cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa gaatgcgaaa
     1741 acattagaga gaagactcca aaaaatagga gaaacaacca agccgcagca gatgaaacaa
     1801 atccgttttc cagtaccaaa atctcttgaa ctccacagcc gctatcccat catgggacaa
     1861 aatgtccaat tggaaagaag tgggagaaca ttactggtaa atggtgattt tcagttttct
     1921 ttaggtaaaa aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac cttattagaa
     1981 catatccgca aacaaggaga aggaatcctt ctctctccga aagtaagctt tcaagtatat
     2041 cagcaaaagg gttatcaaat gacatctgaa gaatccatca ttcgttttgt catgagacaa
     2101 acagagtttt cagaatcgct tgtccgtagt ttgctgaatc acttagggtt tgctcaggaa
     2161 actttgacga aaccgttatg tacattaagt gggggagaag cgacccgtct gacgattgct
     2221 ttgcttttta ctaagccaag taatgtgttg ctgttagatg aaccgactaa ttttattgat
     2281 atggcaacga tcgaagcttt agagaagcta atgcaaatat atccgggaac gattttgttt
     2341 acttctcatg attcttactt tgtcgagcgt acggctgatg aagtttatga aataaaaggg
     2401 cagaaaataa aaaaagtact tacgagaaat ttttaaatgt gctacgatga ttttcgtgac
     2461 aaatttaaag gagtggtaca gattgcctac aaataaaaaa gaaggaataa tttttacaac
     2521 aatgatgtgt tttttgatgg tcttggggat gagcgcgtat aatttatggc tgcatcaaga
     2581 tcttcattta tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg ctttcatttt
     2641 ggatgtcttc atcatcggtg tgctggcgaa aaaaattgcc tttaaactac cagtaaatcc
     2701 agaaagcaag gtccaaatga tcttaactat ttcatgcttg atggttttag gaatggtgac
     2761 atgtatgtct ttattcggtg tgatcatgga acaaggaata cctgatcaat tattctctgc
     2821 ttatatgaat gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt tgatcatcgt
     2881 tggcccgctt tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat ggaacactgt
     2941 tatttgaaaa aaggccttgg catgcttttt gtcatagtct cttgaatgca gttctagaaa
     3001 aatagtgatc cgaacgatag gttttggcct gtcgttcgga tcgctttttc ttttccaact
     3061 gtattgctct tttattgaag ataatttgct cttcaataga tatttctaga aaggttctgt
     3121 ttatccctta ctcaaaaaac tgctgcattt gttctcttac ttgctgacac cattggagtt
     3181 cctgctgaca aagcataatt ctgcgctgga tcacaacata acgtccaaat tcttgtttct
     3241 tgtctagcga gaggtcattc aatttttttt gatttttttg catgattttg ttcagctgtt
     3301 gttctctagt gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa aaagcataga
     3361 tttttgctag aaattcatct ttttgcgtag gagcgggagt ttcctctttg atccattcct
     3421 ctacaagagc acgtccttca tcagtcaagt ggtagatctt tttttgagag tgctcgtctt
     3481 tgtccaaaac ggtcacataa ccctgctggt ctaacttggc aagcgttgta tagatctgac
     3541 tgtgatgggc ttcccaaaaa attgtgatca gctgcttcaa ttcataccca gaacgtggtt
     3601 tggataaaag cacgcataat aatatatagg ctaaagtatt cataaaaatc ctacctctcg
     3661 tcttattttc tttattatat cacagtaaag tttccacgtt ttacatgtgc atatttacat
     3721 gtaaaataaa tcatgtatga tgctaataga gagaaaggga tggaaatatt ggagaatcaa
     3781 atagatatac aagaacaaac aaatgaagaa acaaagggga aaagttcagc gctagttgta
     3841 gcagctacct gtatgatcgc ttttatggga gtgggtctag tagacccaat cttaaaaacg
     3901 attgctgcac agttaaacgc tacgccagct gaaacgaccc tgttatttac tagttacatg
     3961 ttagtcactg gtgtggtgat gctatttaca ggtttcttat ctagtcggat cggtttgaaa
     4021 aaaacattga tgacaggatt gtttatcatc gttgtattcg ctggtcttgg cggattatct
     4081 gggaatatcg gaatgctgat tgggttacgt gccggctggg gtgtcgggaa tgctttattt
     4141 gtctctactg ctttagcaac gatcgtcagt attttagttg gtaacacaga aaaagcaatc
     4201 atgatgtatg aagcagcact tggctgcgga atggctgttg gccctctagt cggtggggtc
     4261 ttgggcagtg gttcatggcg ttatccattt tttggggtag catttttgat gttttgcggt
     4321 tttatagccc tagcggtact tttacctgaa actgagaaac caaagagaaa agtaggcctt
     4381 tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt cagttggact aatcgcattg
     4441 ttatataatt tcggattctt tactttactt gcgtatgcgc catttttatt gattggctat
     4501 tctgaattag aagtcggttt tgtatttttt ggctggggtg tgcttctggc tattgcttcg
     4561 attttcgttg caccggctct tgagcggaag tttacgactt atcggacaat tttagcagca
     4621 tttgtcttat tcattatttg tttgctgtta ttaggagctg gaacttctat gccagtcctt
     4681 gtaccgatca gcattgtgtt agcaggattt ttccaaggaa ttatcaatac actgttgacg
     4741 acgattgcta tggaaatacc aggtctgcaa cgaaacgttg cttcgtccag ctatagtttt
     4801 gtccgctttt tcggtggagc tttagcacca tttattgcag gtgtgattgg tgaacatatt
     4861 ggtgaaagct atgcgtttta ttttgcaggt gccattgttc ttatcagtgt atgttgtatc
     4921 atttatcacc gtaattattt tgctacaaat gaagaagaat aagaaatcag aggttgtgaa
     4981 aaagctgtcc tgcatcaagt cataagaaca gccaaacaaa aatagcttct catatttttc
     5041 gtttggttgg gcttattacc gcagatgcaa gcttttgaac gccgtttacc aagaggttgt
     5101 gagactgtcg tttaactctg aatcacaagg aacaatttca aaaaacagtt tctcatgttt
     5161 gttgaatatt gtgacttatg attgaggagt tggcagaaga acaccattta ttcggaaaaa
     5221 ggggatgtga cacgattttt gtcacattcc ctttttttct caaaataatg aaactggaaa
     5281 attttcagta ggtaagctaa aattcattta agaaaaagaa tggggggata atagatgctg
     5341 gaagaatatt ttcaggaagt cgggttgaca ctcgttgaat tacccattga attttcagat
     5401 gatcggttga tttatcgtgg ctatatcaaa gaattgccaa taatcaatgc ggaaggtgaa
     5461 actagaaaag cgatgcagca aaaaatagct ggtatgtatc aggtgtatcg ggaacagcta
     5521 ctggctgaat cagcggaaga agaaaaagaa aaaacgatga acttgtccat ggatgagctg
     5581 cttcgttatt atgatggcga aacatttgac ggattttcgc ttcttaacga agaggaaaca
     5641 gatttaccag gcagctggta ggcgaacgcc gtttatatgg aaaaagaggc tgtgacaaaa
     5701 tctttgtcac agcctctaaa aagtccggca gttttttgcc gatcttcaag gcactctact
     5761 tatcgtacgg atatttttta gcggaaagtc cgattcaagt ggtttttatt cagctgatat
     5821 ttataagata agtgaaaaat cccagcaatc agtagcgtaa tcgtataagc gattgaatga
     5881 tcgaaaaaag aatagtaatt gattgtcgct aataagcata atgcaaccat gtaactaatg
     5941 tgcaagcgtg taaccagccc tttttgcccg attttgtcaa aatactgcga ccatttttcc
     6001 cgttgactcc ctatgcttcg ttttttgaat tgaaagaaaa gggataaaat accgactccc
     6061 agaaaagcag cgatattcag agaaataagg taaataaaaa aaatcattaa aatttcaaac
     6121 atattgacac ctttctagtt ggatagaacc tttacagaaa aaaggcaaaa ggcgggaata
     6181 atgtttcaca cgaaacatta ttcgcggatt tgtccatcgc cataaatgat atattttgtg
     6241 gaagttaatt catttaaccc cattggacca cgggcatgca acttttgagt agagatacca
     6301 atctctgcac caaaaccaaa taccgatccg tctgtaaaac gagtggaggc attgacatag
     6361 acagccgccg cgtccacttc atttaagaaa cgttgactag ccgcataact atctgtcacg
     6421 atcgtttctg aatgtttagt attatgccga ttgatatgtg caattgcttc atccaaagaa
     6481 tcgaccactt tgacagcaag gatataatct agatattcag tatcccaatc tgtgtcttct
     6541 gctaaagcag ccgtctctag atattctaac gcacgagcat ccgcacgtaa agagaccccg
     6601 tgttctacca gttctttttc aatagctggc aagaaaaaag gtgcgatctc agaatggatc
     6661 agcaatgttt ctgccgcgtt acagacagat ggacgggaag cttttgcatt gacgatgatg
     6721 ttcactgcca tagataactg tgcttcttta tcgatatata cgtgattatt tcctgtacct
     6781 gtttcaatga taggtaccgt tgcctgttct ttgactcgtt ggattaattt tgctccgcct
     6841 cgaggaatca gcacatctaa gtattctgtc aatcgcatca actgtcctgc agtttcatga
     6901 gaagtatcat cgacaaactg gatggcataa ggagattcgc cagcactgac cagtacttgc
     6961 tgcaataatt ggactaataa ttgattcgag aagaatgctt ctttgccacc gcgaaggata
     7021 acggcattcc ctgatttgaa tgctagacta gctgcatctg tcgtgacatt tggacgagat
     7081 tcgtaaatga tgccggtcac gcctagtggc acgcgctttt tgccgattct caatccgtcg
     7141 gtgttcgtcc acatctcatc tacttttcca ataggatcgt ctaacgaagc aattttttcg
     7201 atctctgtag ccatggcatt gattcgctca ggtgtcagct gcaagcggtc ttgcatcgtt
     7261 tcactgatat tgttttcgac tgctttttct aagtctttat gatttgcttc aatgatcgct
     7321 tgttgattct ttttgatggt ttgtgccatt tgaacaagta gctcattttt gcggtttgta
     7381 ggcataagtg ctagttgttg cgcgctatct tttgcctgtt gtcctaattg catcaagtca
     7441 gtcatttcaa gtcctcctta aataaagtac ctacttcttt tcctgcaaga atatcaaaaa
     7501 taattgccgg attttttcca tttgctaaaa tcattgcgct gtttgcttca aatatcctag
     7561 aagcggcttt taatttactc tgcataccgc ctgttccaaa tgtgctacca gcaccacctg
     7621 cttgtgccag aatcttttca tcgattttat cgatatagcg atacatctga gcctcgggat
     7681 ttgtagtcgg attatctgaa taaaacccgt cgatatcaga aaggacgatc aaaaggtctg
     7741 ccgcgacaag ttttgccatg atggctgaca gctgatcatt atcgccaaat tttgtcagat
     7801 gatctaattc atccactgct acagtatcgt tttcgttcac gatagggatc gtgtccataa
     7861 tcaaaagctg ttccaatgta ttcctcacat ttttacgact ttcaggataa tctaccacgt
     7921 ctcttgtcac gaggatctgt gccgtctgtt gattatatac actgaatctt tgattataga
     7981 tattcatcag ttcggcttga ccaacagccg ccgcagcttg ctgttcagga atcaaagtcg
     8041 gacgttctga caaatgtaat ttatgcatac caacgcctac agctcctgat gagactaaga
     8101 tcacttcctt tccccgattt ctaagatcag aaaggacaaa agctaactga tcgattgccg
     8161 caagattgat atttccattg ggataaatca atgtacttgt tcctactttg acaataattc
     8221 gttttgcatt tttaatttct ttacgcatgt gacacctcaa tcaaatatcc actcattaat
     8281 ttcgttctat tctacttgtt ttgacgagta aagtcatgta aaaaatagtt tttttgtaca
     8341 acttgtagtc gtttggaaaa agaacgaatt aatggtttac tttaagatag cagaatttct
     8401 tgaagaattt gtaaagaaag tcttttacag cctcggatat gttagaatag gagtagttt
//
LOCUS       DACDHL010000102         8451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDHL010000102 DACDHL010000000
VERSION     DACDHL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.103"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..193
                     /locus_tag="IV228_002437"
     CDS             <1..193
                     /locus_tag="IV228_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718290.1"
                     /translation="LVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIF
                     YIGYFPNKNNNFYFYRNKL"
     gene            complement(319..996)
                     /gene="nth"
                     /locus_tag="IV228_002438"
     CDS             complement(319..996)
                     /gene="nth"
                     /locus_tag="IV228_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8718291.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1003..1701)
                     /locus_tag="IV228_002439"
     CDS             complement(1003..1701)
                     /locus_tag="IV228_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8718292.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1969..3153
                     /locus_tag="IV228_002440"
     CDS             1969..3153
                     /locus_tag="IV228_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8718293.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3159..3602)
                     /locus_tag="IV228_002441"
     CDS             complement(3159..3602)
                     /locus_tag="IV228_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8718294.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3828..4115)
                     /locus_tag="IV228_002442"
     CDS             complement(3828..4115)
                     /locus_tag="IV228_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718295.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4286..5638)
                     /locus_tag="IV228_002443"
     CDS             complement(4286..5638)
                     /locus_tag="IV228_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8718296.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(5910..6356)
                     /locus_tag="IV228_002444"
     CDS             complement(5910..6356)
                     /locus_tag="IV228_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8718297.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6592..7518)
                     /gene="cysK"
                     /locus_tag="IV228_002445"
     CDS             complement(6592..7518)
                     /gene="cysK"
                     /locus_tag="IV228_002445"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8718298.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7658..8451)
                     /locus_tag="IV228_002446"
                     /pseudo
     CDS             complement(7658..8451)
                     /locus_tag="IV228_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
BASE COUNT         2293 a         1732 c         1407 g         3019 t
ORIGIN      
        1 tttagttata aatggtattt ttttattatc gcttatcatt tttattacac ctttagatag
       61 atacgtcact tatttatata atcctttgga atactggact tattttatag gtatattgga
      121 aggcattatt ttctacatcg ggtattttcc caataaaaat aacaactttt atttttatcg
      181 caataaattg taataagtta ccatcctagc tactgagtat gtacatttac tttttcatgc
      241 aaaaaagata aacgaaacag cttttctcca taataacaag ataattcata catttatttg
      301 caattaaaga aaccacgtct acaggcgtgg tttctttttt agcgcctttt cttttagacg
      361 catccgattt ttcccatctt gacatattga tagcaaagga catacctcgc acttgggatt
      421 tcttgccgtg caatgatatc taccaaaaaa aatcagtgta tgatgtgtct ttacccataa
      481 ttcttgtggt actttccgca tcagtgtttc ttctacttcc atgaccgtag catctagttt
      541 gcagatcctc aggcgttttg agacacgttc cacgtgggta tctactgcga ttgctggtat
      601 cccgaatgca tcccccaata cgacatttgc tgtctttctg cctacaccag gaagagacat
      661 caattcttct cttgacgttg gcacttgccc atcgaaacgt tcaatcagct gctgtgcaca
      721 cgctttgatg ttttttgctt tatttcgata caaaccaatc gtttttattt taagaatgat
      781 ttcatcgatt gaagcttctg ctaacgcatc gggtgtcgga aaagaggcaa acaaatctgg
      841 cgttgcttta ttcactgata catctgtcgc ctgggcactt aagatcactg caatcaataa
      901 ctcaaatgga ttattgtgct tgagttctcc atgtgcttcg ggaaacattc catacatcgt
      961 ttctagagct tccattgttt tttgtttgct taacatattc cgctactcct ccaaccaatt
     1021 tttcagtgag actttcggca attcttcttg ttgagctgac tgttctactt ctctttgaag
     1081 aagctgctgt tttcttcgtt tttggtcttc ttccacttgt tgttttgtac gtagattttt
     1141 tctttcccac gatagcaaaa tgcggtcaac gtaattaaaa ctataagcct gatttaatac
     1201 ggcttcccgc agtgctagct tcaaaatttc cggctgataa tggtcttctt ctagccattg
     1261 tccgatccgc tggaactcga tcgaagaaag cgggcgtcca aattcttgtt caaataattg
     1321 atacactgac tggatttctt tttcttgtgt tttttcttcg tatttttttt cttgtgtttt
     1381 taaatactca cctaatagat catagatggg gtaaagatta tatcgatctg cttttttacc
     1441 gttgacaatc gtcgtttcta tcttgataaa tccatttgtt acgagacgat ccagtatttg
     1501 aaaaatctga tcttgtttga ttcccatatc catagcaatc atctgcaaat cagggaaagt
     1561 atctccttca agctgtgcca tatgcaactg tagaacaaat aaaaattctt cagattgtag
     1621 accgatccta tgataatttt taagtaaaag attagatatg actgtttgtc cagcgcttaa
     1681 gtaattttct aaattcaaca tgatttctcc tccgctcaat cacagtatac gtaaaaaaaa
     1741 catgggaagc aagaacaggt ttcttttcta tttacttcac tgatcggtca gatttacaat
     1801 gtcgcctact tgatttttgt ttgttcatgt ttttgatgcg tatcttcact tttcggaata
     1861 aataaattaa gtagatttcg ctgttcctta gaatgttgac aaggaaacaa aattactata
     1921 cactattttt gtttccaagt taacattatt gcataaagga gtttttacat gtacagtttc
     1981 acaaataaac attctaaagt ttttggatac gtctctgtct tattcactgg attaggattt
     2041 actatcgtca gtcctgtact tccgttttta acgctgcctt attctcactc catacatcag
     2101 caagctttct atatcacttt gctcatgtct gtttatgctc tagctgcctt cttatctgcc
     2161 cccattctcg gtagcttaag tgatcatttt gggcgtcatc cgatccttat tatcagttta
     2221 ttaggatcaa gtatcggata tttgcttttt ggtttaggaa attcgatatg gatgttgttc
     2281 cttggtcgta tcattgaagg attgacggca ggagaaatct ccaccttata tgcttacttt
     2341 gccgacatca cggaacctaa tgaaagaacc aaagtttttg gatggatggg tgctttagtc
     2401 ggtataggaa caacgctggg tcctattata gggggtctat tagctgagtt ggggaattct
     2461 gttcctatat ttattggagc actctttact ttcttaaatg ccgtttatgg ctacacattt
     2521 atgctagaaa gccttccaat aaaaaaacgt tcagtagatt taagtttctc ccatgtaaga
     2581 ccttttcacc aattacaaca attattcaag atttcatccg ttattccttt attgacagca
     2641 ggttttgctg tttggttggc agctggctct cttcaatcta tcttttctca attttcgatt
     2701 gacgcctttc agtggaaggc tgggttggtt ggtttaagct tctctttgat tggaatcttg
     2761 gatactgtct cgcaattgtt cattatgcct cgattgctca aaaaattttc agaacagcag
     2821 atcacaagaa tagggatgtt cagtgaaatc ctcgcctatc tattcatcac attatctgga
     2881 attcttctac tgccgatttt gtttttattt gggattatct gttatggttt cggtgattct
     2941 atttttactc ctgtattcaa tggccaacta tccaattctg tctcagataa ccagcaaggt
     3001 cttgtaatgg gcggtaccca aagtatacaa tctctatcgc gcgtcatcgg cccgctaatc
     3061 gcaggacagc tttatgctgc agcaccattt cttccgacgg tattcggatt tttgcttatg
     3121 atttttgcct attatcacta caataaaaaa tagtagcttc atttgctact tcgtataaat
     3181 tctctgtatc gaccgataaa acgaaatact gtgtcaaatt cttcttttga catattttca
     3241 aatacagtag catctcgctg tacccatttt tgatggagtt gctcgtgtct attccttatt
     3301 ttttgtccga ttggcgtcaa ttgaaaatag atttcttttt tattctcttc gctttgataa
     3361 gtgtttatga tccctctagc tatcagtttt tttgttagct tactgatggc tcctctagtc
     3421 atgtaactag cattcgctaa ttttgtcacg ttaggatcag ggattttccc gatatgttca
     3481 atcacttcta gttcatttag agtaaatcct gcaaattctt tttccatttc tggtttatta
     3541 cgatgaataa tctcattagc taaatccaga tattcttcta ttagcagttg tcttttatcc
     3601 atttctacac cttctttttc tttcattata ttgttttttt gtttcctatt caacaaagat
     3661 aaaaatattt tgtaggaaaa cccaatctga aaccaagaaa aaaaggggtt gacaaagctt
     3721 gtcataaccc ctttttctga ttaaaagata gtaaagagac agtcatcggt aattgtgatg
     3781 attttttgtc atcacttaag gatgagcggc cttttcaaaa actgtcttta aagatcatca
     3841 tcagtttcta gcacatatgg ctgaccttca tatgtgtact ctgtattcaa tgtgccgtca
     3901 ttttcatctg agtagatgcg tctactgaaa ggaaaacgat gagaaacatt atctttggcg
     3961 tcatttgctg gcatcgtgta atattcctct tgcgtttcat tcaactgaga gagtacttga
     4021 tctgccaact catcaatatt gatcgttaat ttcttcgaag cacttttata atcttctaaa
     4081 aatgcatctt gttggacaaa atattcttta ttcatcttct ttctcacctt tcttttcaaa
     4141 atcaaataaa gctgcttgtt cttttatgta acagctttat ctttgctgct tattgtgaca
     4201 aaaaattcga agaaagacaa ctaattgtac ttttcacaaa aaaagacaga gggtcgtgac
     4261 aaaattgtgt cacaaccctt tttctttatt gtgcagtttc gatttcgcct agcgcttctt
     4321 gtgcaagtag gttcaagtaa ttccatgggc ggtcaaaatg tggttggaag aagaaatctg
     4381 aaagtgcgag atcttctaca gtcattttat tttgaacagc taaagacatg gtatttgctg
     4441 attgtgtaat gtcgtatttg gacatgaact gagcacctac gatacgattc gttccttttt
     4501 catagactag ctccatcatt actttttcag tagttggcat gaactctgga cgataattat
     4561 cttcaaatac agtcgcttgg acttctagat cgttcaaagt tgcgctttct ttggtcacac
     4621 ctgtcgaacc gatcgtccag ccaaataggt aaagtcctga agttccttga gtaccacgat
     4681 acgccatttt atgatctgtc aagttatttc ccacaagtaa accttgacga acggcatttg
     4741 ttgccaaagg aatatagttt tgcgtgccac ttggattgta gttgactact gcagagtctc
     4801 cagctgcaaa aatgtctgga tcacttgtct gcatgtattc attcactttg attgccccgt
     4861 ttggcagtgt atctactttt ccttcgatca gttttgtatt tggacggaag cccacacata
     4921 aaatgaccat atctgtatca aatgtatctg ttggtgtagc tacttgtttc acttttcctt
     4981 cttcatctgt gataaattca gtaacatttt cgcctagcgc aagagtaaca ccgcggtctg
     5041 tcaattcttt ttctaagata tctgtgaatg gtttatctag atatttattc aagatgcgat
     5101 ctaatccatc aatcaatgtc acttgttttc cagattcgac aaaagcttct actaattcaa
     5161 taccgatata tccaccacca acaattacta cacgttttgc ttctttcgct tgtgcaataa
     5221 tttcatttgc ttgattgtag tttttgcata ggagtacatt tttcgcatcg attccactaa
     5281 tcggtgggat aataggccat gaaccagttg tcatcactaa tttatcataa gaaaccgttt
     5341 ctgtttctcc tgttttcaga tcttttgccg ttactgtttt attttctgta tcgacgtctg
     5401 ttacatcgtg ttccatttta actgttgctc ctaatgaaga taattcttca ggatttgagt
     5461 agaacaagcc tgctgggtcc tttactactc cgccgacata aagtgcgata ccgcaagata
     5521 agaaagaaat attgtcattt ctttcatata ctgtcacttc tgcttctgga tgatttttta
     5581 aaatactttt tacagctgca gtacctgcat gagtacagcc aattactact actttcattt
     5641 atggtccctc cgattaacat tttgtccttc gacaaaacta ctataacaga aataaaacgg
     5701 atacaacagt ttaatgcttg tgaattcaca aaggtttgtt tgtagtttca ttccttttca
     5761 agcataagaa cccaatatct acaagattct tattgtgata atattcactt tgatatctaa
     5821 gattgtgaat aaaagacaaa aaaataaaaa agctagcgtt tttttacaca gctagctttt
     5881 tttaggtata acacttatag aaactgtttt tacatttgcg ccttttttgc tgactcttta
     5941 caatccggac agagtccata caattccaac cgatgttcat taatctcaaa atcagtcagt
     6001 ctgctcgcag ccatttctat atcttcaagc cctgggtaat gaaaatcaac aatcttaccg
     6061 cagttttcac aaattacatg ataatgacgc tttgaactga aatcaaatcg gctagaagca
     6121 tcgccgtact tcatttcttg aacaaatcca atcgaagtaa ataagcgaag gttattgtaa
     6181 acagtcgcaa cactcatatt tggaaaccga tgttcaagtg ctttataaat ctcatcagcc
     6241 gttggatggg aatgatgttc aatcaaatat tctaaaaccg cgtaccgttg aggtgtgatt
     6301 cgaatgttct ctttttttaa cttttcgata acctcttcga ctaacttatt ttccatgtaa
     6361 acaccccacg ttgttattct cgtcttatca tactattttt acattttaaa atccacttaa
     6421 catgaaaaga aaattcaatt ttattgtatt ttaatgaaaa aagacgtaaa acagtcgcgt
     6481 tcactatttt acgtctctat tcaaataatt tttctcatca taaatttttg tgtgttcttt
     6541 tcatctttaa aagtttttta cataaaccta tctggagaaa aaccaagtgc cttatatctc
     6601 ttggtaaagg gctgtagata aataacgttc accattgtcg ggaaccacag ctaatacctt
     6661 cttgcctttt cctaattctt ttgcgacttt tagcgcggca gcaattgcag cgccagatga
     6721 gatgcctact aaaatccctt cttttcgccc aacttctcta gctgtttcca tcgcttcgtc
     6781 tccactaata gaaaggactt tatcatacac agaagtatcc agtgttttag gaacaaaacc
     6841 tgtaccgatt ccttggattt tgtgaggtcc cgcttctttc ccttctaaaa tagcagattc
     6901 tgctggttct accccgatta attcgatttt cggatacaca cgttttaatt ctcggccagc
     6961 gccagtgatc gttcctcctg taccgatacc tgcaacaaac gcgtccaagc catttactcc
     7021 aaatgcttga tgtatctcag gaccagtcgt cttctcatgc acaagaggat ttgcttcatt
     7081 ttcaaattgt aatggtaaaa agtagccgtt ttcttttgcc aaacgttctg cttctctgat
     7141 tgatccagaa ataccatccg caccaggagt caaaatcaac tctgcgccgt accctttcat
     7201 caataaacgt ctttcgatac tcatcgtctc aggcatcacg atgattactt tatatccttt
     7261 tgcaactcct accatcgaca atccgattcc tgtatttcca gaagtaggtt caatgatagt
     7321 atcacctggt ttcagtagac catcgtgttc tgctttttct atcatactta aagcaatacg
     7381 atcttttaca cttcctcctg gattaaaaaa ctcaagcttt acaaaaacat ctgctgaatc
     7441 ttctggtacg attttattta atttgacgat tggcgttttg ccaatcaatt cggtgactga
     7501 gttatagata gctgtcatct tcaattcctc cttgtctttg ctcttcttac ttatcataat
     7561 acaatctgtt catttatggc gagtgattcg tctttgacta gcaaaaaaaa gacaaagata
     7621 taccatatat ggtagtttct ttgccttttt atctaaatca aacgtttagt tcttgaccaa
     7681 ctaataaaga ataactggac atagacatgc catttttcgc cattagatca tcaacagaca
     7741 tgccaaattt ttgtgcaata tcccatagcg tatcacctga cacgactgta tatgtctgac
     7801 cattatttgt atttacatct gttgttgaag atgatgacgt tgaagtcgtc gtagtagtcg
     7861 ttgttgttgt acttgtggta gccgaacctg ttgatggcgt atcaaaacgg gttaaattat
     7921 aagtattaat caatgagttc aatttggccc cataatttgg atcggtagca taacgacctg
     7981 tcaagtacgc tgttgcatca taaaaacttg ttgtattgct tttccacacg ccagcataat
     8041 gataatcgcc cgtggaaaaa ctggttgaac gaagaacatt cgcatggtct tggaaggatt
     8101 cccaataaga agaatatttt cggaatggtt cattcatcgt tacccattgt ccgttacata
     8161 ttctttggtt gccatataaa cgacctgtcc gttatagctt cctttcacac cgaatagatt
     8221 ataatttggc gcactagcta atccggaagt gccatatgca ctttccaaca acgcttgagc
     8281 gatcatgact gaagcataca agtcattact tgcagcaact tctgacgcag accagccgat
     8341 ttgatcgata aacgcttgag gatcagcaac tgtttgagat ccttgttcat ctgcatgaac
     8401 atgttgcggt accgctgtaa ccatcatcgg cccaactaaa agactcgttc c
//
LOCUS       DACDHL010000103         8421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDHL010000103 DACDHL010000000
VERSION     DACDHL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.104"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            565..2103
                     /locus_tag="IV228_002447"
     CDS             565..2103
                     /locus_tag="IV228_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8718299.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2100..3065
                     /locus_tag="IV228_002448"
     CDS             2100..3065
                     /locus_tag="IV228_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718300.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3052..4374
                     /locus_tag="IV228_002449"
     CDS             3052..4374
                     /locus_tag="IV228_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8718301.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4437..4541
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4615..5358
                     /locus_tag="IV228_002450"
     CDS             4615..5358
                     /locus_tag="IV228_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8718302.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5373..6365
                     /locus_tag="IV228_002451"
     CDS             5373..6365
                     /locus_tag="IV228_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8718303.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6377..7114
                     /locus_tag="IV228_002452"
     CDS             6377..7114
                     /locus_tag="IV228_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718304.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7104..7826
                     /locus_tag="IV228_002453"
     CDS             7104..7826
                     /locus_tag="IV228_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8718305.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7910..>8421)
                     /locus_tag="IV228_002454"
     CDS             complement(7910..>8421)
                     /locus_tag="IV228_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8718306.1"
                     /translation="LSTISQKYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSA
                     QAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAK
                     EWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAK
                     AFWEANGWY"
BASE COUNT         2710 a         1446 c         1823 g         2442 t
ORIGIN      
        1 tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt ttgatgaaga
       61 accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt tatggagtga
      121 aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac gtttatgatg
      181 tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt ataatattcg
      241 ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg gctgtttctt
      301 tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag tgacaatttc
      361 gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg atccgacttt
      421 gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt tttttgttcg
      481 tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg tttatactta
      541 aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag tgaaagaaaa
      601 ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc tttgctgatt
      661 gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa aatggcagaa
      721 gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt attaggttta
      781 cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt tatttacgct
      841 ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc gtctattgaa
      901 gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt agagcttccc
      961 atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat catcggtacg
     1021 gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt gttaggtatt
     1081 gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt gcttgcaatt
     1141 atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc catcattact
     1201 ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc cttgtttaaa
     1261 aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct gatccaaatg
     1321 tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc gaattttggc
     1381 aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta tcctgagttc
     1441 acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag tgctgatttg
     1501 acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt cgatatgaca
     1561 ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag atcttttgcg
     1621 aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca gatcaaagcg
     1681 ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca agaagcatat
     1741 ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca agcaatcaat
     1801 aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa ggagtacgat
     1861 ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc accgctgatg
     1921 aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa attaagcgga
     1981 aaaataacag aggatcaaat gattgatatg aattaccaag tcaatgtcga aaagaaaaag
     2041 ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga ggaagatcag
     2101 tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg
     2161 atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt ccttccggta
     2221 gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca gcaggagaca
     2281 tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg
     2341 gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa aatattgaag
     2401 tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta gatgaacttc
     2461 ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga gagttgtctg
     2521 gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca gatgtgattt
     2581 tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta caagaattgg
     2641 ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac aatatggaag
     2701 aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt atccaatgtg
     2761 acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat ttctttgatg
     2821 aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga tatttcttaa
     2881 atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg aaagaagtat
     2941 attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga agtattgggt
     3001 cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga aatgaaaaca
     3061 tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa
     3121 gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac ttgtcctaac
     3181 cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa
     3241 caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga acttcaaaaa
     3301 ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt agcagaagct
     3361 gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat tgaagtcaac
     3421 gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc tacgatctta
     3481 ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt gcctgtactg
     3541 cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc tacgattgcc
     3601 aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt aaaagaattt
     3661 gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat tcagtttgct
     3721 tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt agtcggaaaa
     3781 gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc gaatacagaa
     3841 tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc agtcaatgat
     3901 tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc aaggaaaacc
     3961 ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg gataacggat
     4021 gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc cagtcctaaa
     4081 cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg
     4141 gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg
     4201 acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca agcagacgaa
     4261 ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga gttggatagc
     4321 tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa ataatcagca
     4381 tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa aatgaatacc
     4441 tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac ccattgaacc
     4501 tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg ttactatgtc
     4561 gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa caaaatgaag
     4621 aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca gattgttgtc
     4681 atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt ttctgcatta
     4741 gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga agcagtgctt
     4801 ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga ttattggaag
     4861 tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc aatcatggtg
     4921 ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt gatccttgtt
     4981 gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag gaaagtatca
     5041 aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat ttatcgaata
     5101 tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc tgctacttat
     5161 tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt agggtattac
     5221 atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat catttgtgtc
     5281 attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg gtattatcgt
     5341 atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt ttattaccgc
     5401 tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa caagaaataa
     5461 aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat catactggca
     5521 tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc caaatcatcg
     5581 aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca caatttggtg
     5641 taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata ccagtcaaag
     5701 caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca gacagtaaca
     5761 tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag
     5821 aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa ctgacaatgg
     5881 taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc caatggtact
     5941 ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac tatatcccat
     6001 tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg aatgatcaac
     6061 tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa aaaggctatc
     6121 aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa
     6181 atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat acggatgatc
     6241 ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct tttatgcagg
     6301 aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat cagtttataa
     6361 aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca ttgaaaacct
     6421 gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat cgattgtcgc
     6481 gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc agcctatgac
     6541 ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag catttagtta
     6601 tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg ttacgttata
     6661 tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc tggatatctt
     6721 tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga tgaagcagcg
     6781 aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt
     6841 tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga agctgcctaa
     6901 aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa tttatttatc
     6961 agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc
     7021 tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc aaacgattta
     7081 tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta gatgggagtc
     7141 agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat tgtgattcgc
     7201 cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc ttaagttacg
     7261 gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct tttttgaaaa
     7321 aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta ccgatgacta
     7381 cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag aaacctgttg
     7441 tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct taccctaatc
     7501 actatttgat ccattggtat tcttctttgg actatcaaca agactatatt gatttaggct
     7561 gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa ctagctaaag
     7621 cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca gagtgggctt
     7681 taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag ttaaagacaa
     7741 tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat caaaacttgg
     7801 aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg tcgcacctct
     7861 ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct tagtaccaac
     7921 cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt
     7981 gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa gaagaatcta
     8041 attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt gattcttttt
     8101 gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt agcagcagtt tctgttacag
     8161 caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga accgtttctt
     8221 gtacaacagg ttgttcagct tggactggtg cttcttgaac ggctgcttga ggttcagcag
     8281 ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagtaa atcaggttaa
     8341 tatcagaaat tgaatttgat tctgcaatgg cattgatcaa ggagttatca ccaacgtatt
     8401 tttgagaaat cgttgataga g
//
LOCUS       DACDHL010000104         8335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDHL010000104 DACDHL010000000
VERSION     DACDHL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.105"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            107..1248
                     /locus_tag="IV228_002455"
     CDS             join(107..335,335..1248)
                     /locus_tag="IV228_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8718307.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="IV228_002456"
     CDS             1483..1935
                     /locus_tag="IV228_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718308.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="IV228_002457"
     CDS             1949..2638
                     /locus_tag="IV228_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718309.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="IV228_002458"
     CDS             2763..5114
                     /locus_tag="IV228_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8718310.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="IV228_002459"
     CDS             5194..5589
                     /locus_tag="IV228_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718311.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="IV228_002460"
     CDS             5914..6531
                     /locus_tag="IV228_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718312.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="IV228_002461"
     CDS             6572..6871
                     /locus_tag="IV228_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718313.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="IV228_002462"
     CDS             6905..7072
                     /locus_tag="IV228_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8718314.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..7587)
                     /locus_tag="IV228_002463"
     CDS             complement(7117..7587)
                     /locus_tag="IV228_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718315.1"
                     /translation="MEFREGAFDFQTSQQNPSLDRLGRDYEDIKNTVAFMKQKKVALK
                     ILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKG
                     RPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(7593..8273)
                     /locus_tag="IV228_002464"
     CDS             complement(7593..8273)
                     /locus_tag="IV228_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HAP8718316.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
BASE COUNT         2813 a         1301 c         1642 g         2579 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgatggg ttctgttgcg aagtttgaaa
     7561 atcaaacgcg ccctctctga actccaaata tcttaggctg ttattcccat taataccttg
     7621 atttcagtag acaccgaaaa gccgaagagc gttccatttc ttcggttctt tttatatatt
     7681 cctcgaatgg tctccatgcc cttaatcgtg gaagaggctg tacggagact ttgataaaat
     7741 ttatttcgtc gtttaatagg tcgatggtct tgttctatta aattgttaag atacttcaca
     7801 gttcggtgct ctgtcttagt atataaaccc acactctgta actttctaaa ggcggagcca
     7861 agagaaggtg ctttatcggt cacaattgct ttcggctcac caaactgttt atggagtcgt
     7921 tttaagaaag cataggctgc ttgcgtatcc cgtttctttc gtaaccagat atctaaggtt
     7981 aagccgtccg catcaattgc acgataaaga taatgccaac gtcccttaat tttgatatag
     8041 gtttcgtcca ttttccatga atagaaggat tgtctatttt tcttcttcca aagataatag
     8101 aggactttgc tgtactcttg cacccaacga taaatcgtag tatgacaaac atttattcca
     8161 cgatcatata acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca
     8221 acagcgacaa taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg
     8281 tcctctctgt cttttttctc aattttacac taaaatagat tttttggaaa acttt
//
LOCUS       DACDHL010000105         8325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDHL010000105 DACDHL010000000
VERSION     DACDHL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.106"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..483)
                     /locus_tag="IV228_002465"
     CDS             complement(<1..483)
                     /locus_tag="IV228_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8718317.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     S"
     gene            complement(613..1323)
                     /gene="budA"
                     /locus_tag="IV228_002466"
     CDS             complement(613..1323)
                     /gene="budA"
                     /locus_tag="IV228_002466"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8718318.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1336..3000)
                     /gene="alsS"
                     /locus_tag="IV228_002467"
     CDS             complement(1336..3000)
                     /gene="alsS"
                     /locus_tag="IV228_002467"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8718319.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3205..3867)
                     /locus_tag="IV228_002468"
     CDS             complement(3205..3867)
                     /locus_tag="IV228_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8718320.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3877..4692)
                     /locus_tag="IV228_002469"
     CDS             complement(3877..4692)
                     /locus_tag="IV228_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8718321.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4954..5397)
                     /locus_tag="IV228_002470"
     CDS             complement(4954..5397)
                     /locus_tag="IV228_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8718322.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5531..5869)
                     /locus_tag="IV228_002471"
     CDS             complement(5531..5869)
                     /locus_tag="IV228_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8718323.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5857..6234)
                     /locus_tag="IV228_002472"
     CDS             complement(5857..6234)
                     /locus_tag="IV228_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8718324.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6458..7651
                     /locus_tag="IV228_002473"
     CDS             6458..7651
                     /locus_tag="IV228_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8718325.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7817..8239)
                     /locus_tag="IV228_002474"
     CDS             complement(7817..8239)
                     /locus_tag="IV228_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8718326.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2444 a         1685 c         1408 g         2788 t
ORIGIN      
        1 tgaaaaagca tttatattaa aatttgctaa taaaagatct acatattttt ctagaaatgc
       61 ttgatcgatt tcatcatacc ctttcgttac tccgcaatcg atatctaaaa caccgattaa
      121 attaccttct tttaccattg gcactacgat ttctgattgt gcggctgaat cgcaggaaat
      181 gtaatttgca tgggttttga catcatcaac gatcaatgtt tcttgttttt cagctgattc
      241 tccacaaact ccttttccca tcttgatacg tgtgcaagaa actcgccctt ggaacggtcc
      301 taaaatcaat tcattaccat cgaataaata atatcctgca aagacagtct caggcaaagt
      361 ctcattcaac aatgcagatg aatttgctaa atttgctaac cagttatctt ctgcttctaa
      421 taatccttgt tgctgtaata ataaaatttc gtatgcatct tttttctgtt tttctgtcca
      481 catgataaaa accgctcctt tttgatctat ggagaagatt atagctcagt ttttttgtaa
      541 acgctaggtt ttctcctcaa aaaaacgaga tacgtcaggt taatcacctt gaacgcatct
      601 cgctgtcttt ttctattctg cttgttggat ttctgctgct aaatcagcat aatcaatcgt
      661 ttcgtgaaca aaagtcggat catcaactgg aaaatgctgt acaagggacg agatgcggct
      721 aatctcaact tttccttcag ccacttcaaa atccattaca tgtccgccaa atgtatgtgt
      781 atcatcgata aaatgcaggt gaaatcccgc tgcagcaact ccttggaata attcaggcgt
      841 aaagaacccg actaatgttc cttggacatt gctttgttgg aattctggtt gagactctga
      901 aatttttgct agtcttctgt aaggtttttc ttgcttcggc atgatacgga tgtgcatatt
      961 tttgaataat ccagttatct tcactgccaa aaataggttt tccccggctt ctcttactaa
     1021 tatgtctttt ttcaattgtt ctccatctgc agtttttgag acagaaaaac tagtatctgg
     1081 cgtaaaatct gtaattgctg catagggggt aagttcactt ccatctaatt gggcaactga
     1141 gccatctgat cttccttgat aagctacgcc atctaaaaag atgacttcac catcaagccc
     1201 atgcaatgtt cccaatccca gagttccttt ttctaatata tgagcaatcg tttctgttcc
     1261 atccatcaag ccagccatca aagctcctaa tgtcccgtgc tggtataaga ttttttcagt
     1321 catgtcgaca cttccttaat agaattgatc tggtaagatt gtttctccca atttctcatt
     1381 atcacggtaa tcaatcggaa tatcaataat tacgggtcct tctgtcgcaa atccttcttc
     1441 taaagctttt tctaattcct ctacacttgt agctcggatt cctttagcgc caaaagcttc
     1501 tgcatatttc acaaaatcaa ctggaccaaa gtctacacct gacgcccgct gatatttcat
     1561 tttttcttgg aactctacca tattgtagtg tccatcattc caaatcaaat gaacgatatt
     1621 caatttttta cgtaccgccg tttctaaatc ctgggcagag aataaaaatc ccccatcgcc
     1681 ggaaactgat acgatttgtg tatttggacg caccaatgca gcagaaatcg cccatggcaa
     1741 tgcaacacca agcgtttgca taccattact gaataacaaa tgacgaggtt cataacttct
     1801 aaagtggcgt gccatccaaa tataatgact acctacatct acggtaacag tcatatcatc
     1861 tgttactttt gactgaagcg tgttaataac ttctaaagga tgcaagatac ctgcttctcc
     1921 ttttgattga acaatgtccc gctccgttaa ttctgcttga agagatgcta aatattcttt
     1981 tgcatcttct gacacttgtt gaggctctaa tgaacctgtc aataaatcaa gagtggctga
     2041 gatatcaccg atcaattctc tttcaggctg cataaaagga tcgatttcag ctggcgcttc
     2101 atcgatcaca atgatccgcg catctttttc cgcattccag tttcttgctt catattcgat
     2161 tggatcatag ccaatagcaa taactagatc acttcgtttc aaaagcatat ctcctggttg
     2221 gtttctaaat aaacccacac gaccaaagaa atgcgcttct aattcacgtg agatcactcc
     2281 agctgcttgg aatgtctcta caacaggcaa tgctgtcttt ccaactaatt gcctgatggc
     2341 taacgtttct ttttcacttg atcctcgcat acctactaat agtaccggta gttttgcttc
     2401 tctaatgcgt tcagctaggt aacggatatc atctgcagaa gcagaaccta atttaggatc
     2461 gctcaatggc ttgatgacat tcccttggac aggtgcatcc actacatctt gcgggatact
     2521 gataaagctc gcgccttttt ttgaactctt agccatacga taagcattgg caatcacttc
     2581 tgaaattgtt tctggatctt ggatttctgc actgtatttt gtgattggct ggaacagtgc
     2641 tgcattatcc atgctttgat gggtcaactt caatagatcg cttcgtttta cttgtccggc
     2701 aattgctaaa actgggtcac cttctgctgt cgctgttacc aaacctgtag ctaagttact
     2761 agcaccagga ccacttgttg cgataactac tcctggctct cctgtaatac gtccgattgc
     2821 ttgtgccata aatgcagcat tttgttcgtg acgagtcacg attaattcag gaccttgatc
     2881 ctctaattca ttaaatacac catcgatttt tgcacctggt ataccgaata cataatcaac
     2941 atgatgatta atcaaacttt ctactacaga tttcgaacct tgtttcattt tttcgtccat
     3001 tgaacatcgt cctttttatg ctttattttt aattattagt tagaaataat cacaaataat
     3061 tgatacagtt tctaaacctg tttcattcaa aagtatagca cgttttattt catttgttta
     3121 gccatatgtt tcaaaaaatg aaaaagaatg acagttttta cataaaaact gtcattctta
     3181 attgaaagtt tcaatttata aagtctacag atccatagtt ctcatcaatt tacgattgtt
     3241 gaagtaaatc aaaccaaata cgatagaagg acctagtaga atacaccatt ggggctttac
     3301 gaaaaagaag agaaacaaac ttgtcaatag taaaaaatct agtaaactca cataccattt
     3361 tttcaacgta tagtagaacg ctatccgtag aacttgattt ggtttcgacg ctggatttct
     3421 cacctgaaaa taccagtagt tgatgctaaa ggaaactgat aaacaagcaa gtaaaacaaa
     3481 caacggaatc aaccattttc cataagaaaa acgtataaaa aagaaacaat caaaaatcat
     3541 tatcacacta actatccaag caaatagcca ataaaaaaat ccctttagcc aaaattggcg
     3601 aaacgtcttt atgaacaaac gtactggtga aaaacttttt tcttttgcta attcttctgt
     3661 catacctatc atcacgatgc aacctggacc aataaaaagt aaagaaaaaa taaaggaaaa
     3721 gacattcctt ccatctatcg acaaaaggaa cgcagctgct atatacaaga tattgacaca
     3781 ccaaaaacaa aggctacttg ccaatgattg atacaaccaa taaaagaatt ccatataaac
     3841 atttttgtca aaacgctgct ctttcataat cacctcctat tccttcattc ctgatgaagc
     3901 gatcccttct acaaaatatt tttgcatcgc taaaaatata aaagcaacag gaagaacaga
     3961 aacaactgag gcagccatga ttaatgcgta ctctgcatca aatagcccaa taaacatttt
     4021 caaccctaac tggattgttt tattttccgt agaagacaaa taaatgaatg gccccatata
     4081 atcgttccat gtattaacga atgtgatgat cgtcaaactg gcaatcgccg gttttgtaag
     4141 aggtaaaatg atcttgcgat agattcccca ctctgataag ccatcgatcc gcgcactctc
     4201 acataaagaa tctgggatac tactgtagta ttgcttcaac aagaacacac caaaagcatt
     4261 gaacgcttgt aatagcacca acgaccacaa cgtatatgtt aaacccactt ttctcatcat
     4321 aatgaactgc gggatcatat aagactgcca agggacagca atcgtgccga tatatgctaa
     4381 aaataaaata tcgcgccctt taaaatttag tttagaaaac ccgtaagcag caaaactaga
     4441 agtaaacaac tgaatcaacg tgatcacaac gcttaaaaaa gctgtattct taaaaaatgt
     4501 cactaaagga atacgagacc aaataaccag ataattctct aaatgccaag ttttagggat
     4561 ccattgaatc ggaatcgtga acacttcatt atttgctttg aatgaagcag aaaccatcca
     4621 taaaaaagga atgatcgtca ataaagctaa tacaatcagc agaatcatac ccaaccattg
     4681 aataaagggc atgttttttg aacgaatttt tgcttttttt ctttcatttc gaacacctcc
     4741 aaagttataa tcttcatttc tttgtaatga ttacaatcca gaacaacggt tattatagct
     4801 gattcatttc cgttcttctt atgtttgtaa gcgcttattt aacaagttat gtaaatctct
     4861 ttattttttt tgatttaaaa atgaaaaaat acaaaagcaa gactgtcata cgtctcgctt
     4921 ttgtattgaa atttcttcga ttagagatac ttattactgt tcaaaagctt ctttgaaacg
     4981 gtgaatcaat tcattgacta caggtgataa aatccgattc tttttccaga ctagcatgca
     5041 gttcgtttca atcattgggt caagcggaac gaatgtcact cgctttgaaa gatgacttac
     5101 agcaccttct atgactaaag cagcacctac ctgattctca attagcgcaa atacattgaa
     5161 aatcaaattg tacgttcctg tgatcagcaa ctgctccttt tttatgccta accagtcagc
     5221 aaacattcgc tgtatttctg gacgctttga gatcaaaaga ggtacattcg caaagtcttc
     5281 ttttgttatc acgtcttttt ttgctaaaac agaattttct aaaatgagca atccccattt
     5341 ttcctttctt ggtaacaaaa tcgtttcgta tttctctgta tctactggat ctagcaaaat
     5401 cgctaaatct aacaggcttt tttctagtca ctccgtgata tcatcacttg ttccgctgtg
     5461 gatatgaaat tgaacgctgg gataatcacg gatcaacttt tctagtaata gtgccaatgt
     5521 attcgaatta tcagcttcca cacagccgat cgtaaatcgg ccgctgaata attgtttttt
     5581 tctgttctcg aattcttgag tggtttgatt cgttaaatct aaaatttctt ctgctcggct
     5641 tttttagaaa ataacctgct tctgtcaaga tcattttgcg gttttcgcgt acaaacagtt
     5701 ctgttcctag ttcctcttct aactccttca gttgtcggct taaggttggt tgtgtaagat
     5761 gtagaacttc tgcggcttga gagattgttc cttcttctgc aattgtccaa aaatatttta
     5821 gtaagcgtaa ctccatagcg atgctccttg atttttttac ttgatacatt ccattttatt
     5881 tctagataac acacgatcat atttcttgat ttttccttgt aaacgttcca aacattcttg
     5941 catttcagca atttttttcg tcaaaagttc actttcttca atcaaaagct tacgccgctc
     6001 aaatacagtt gcttctcctt cgctagatag gcgaatatat tcaatcaaag actcgatcga
     6061 taatccggca tttcgcatac agatcgtaaa gtcgacccat tttaggtctt cttctgtata
     6121 atcacggatt ccgccttttg tccgattaac tggtggaatc aacccgattc gttcataata
     6181 gcgcaatgta tcggccgaaa tgcccttttc ttcactgact tgtttgatat tcatctgatc
     6241 actctttctc ttaatttctt gccactttac ttatgtattt tatttattct acaactttaa
     6301 gtgtactcca agtcaatact catttctatt aaaactaagc tggattatct atatcggttg
     6361 tttgtgtatt atttcaaata aaaaagtctt gtttttttat tcttttttgg atatactttt
     6421 aacaatttag ggaaatttta ggaaaagggg tataaaaatg acaacgtatc agcgaaagtt
     6481 attattatcg acatcggcgg gaattgcttt agagaatatg gatattatgt ttttagcttt
     6541 ttcattatct tcaatgatta cttcttttca tattagtggt acgcaagcgg gtttcatttc
     6601 aacgatcact aatttgggga tgctcattgg aggtattttt tttggcatca tagctgataa
     6661 atatggacgt gtgaaagtat tttctcaaac ggtccttctg ttttcgattg cttctttatt
     6721 gatgtatttt gcttcaaaca tctacttggt ttatctcttt cgctttatcg caggtgtagg
     6781 agcgggagga gaatacgggg cgtgtatgtc cttgatttcc gaaagttttt caaaaaaaca
     6841 aattggacga gcttcttcgg ttgctggtat tggtgcgcag gtcggtgcgg ctttggctgc
     6901 gattttagct gctgtgatca ttccaacgtt tggttggaag atgttgtatg tcgtaggtgt
     6961 actgccggtg ttgatggtct tgttcattcg tcgcggcttg actgaaccaa aggaattcaa
     7021 atctgtacaa tctgccgaaa agaaaaccaa acttagcaga ctgtttgaat caaaacatct
     7081 tgcttggcaa acatgtggcc ttagtatcat ggtaactgtc caaattgccg gctattttgg
     7141 gttaatgaat tggctgcctt caattatgca aaatcgatta gggctttcgg tttctgggtc
     7201 ttcattgtgg atggtcagta ctattttagg catgtctttg gggatgatga cttttggcat
     7261 aatcatggat aaattggggc ctaaactagc cttttctttg tttttaattt gttcatcctt
     7321 atctgtctat ctacttgtgt tagcccatag ccagtggagt ttggtagcag ccgctgtgat
     7381 agtcggctac tttatcaatg gcatgtatgg tggatatggc gcagtgatta gtagtttgta
     7441 tccaacagaa atccgcgcga cggcgaataa tttcattatg aaccttggac gagctgttgg
     7501 tggtttttct tctatcgtca tcggtttctt gatggatcat tatagtttaa ctgccgtaat
     7561 cttgtttttg agcggtattt acctagtcag tctgctggtc ttgacaactc ttactggtgt
     7621 tacagcatta agggaaagct tgcagcattg atagttgagt ctgtaaatga caagaaagag
     7681 gtgtggcaaa agcttgcatc tgcggtaata agaccagcca aatcaaaaac atgagacact
     7741 gtttccgttt ggctggtcgt attacttgat gcagggcagc tttttcaact gctaatttcc
     7801 ttctatctgg cggctactat tctttgacgt ccgtcatttc aaagtacttt actaataact
     7861 tagcataacg aaaaactgtg gatacactcg tatattgagc gtctgcataa tcttccaaat
     7921 ttttctcagg atgttgatac atgaattcaa tcaaatgata attatatgat tttctgtaga
     7981 aatgaagata aataatgtag attgggaagt tctcctgctt ttccatagaa aagcgccgat
     8041 tattctcatt cttcaaaatg accttatcag gatatttttg gaacaattct tctaaatctg
     8101 ttagataatt caagatagac ttacttgata ccttcaattc gttttgtagt tcttctattg
     8161 aacaccaacg ttctgtttca agcagtaaat gaatcatttt aattttcttt tgagaaactt
     8221 ttgttaaaaa agtctgcata ttttctcctc ctgtatctac tttactatac tacatacaag
     8281 aaaaaaaacg gatacaaata tcatattgtc acaccgtcaa tagtg
//
LOCUS       DACDHL010000106         8139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDHL010000106 DACDHL010000000
VERSION     DACDHL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.109"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..312)
                     /locus_tag="IV228_002475"
     CDS             complement(<1..312)
                     /locus_tag="IV228_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8718327.1"
                     /translation="MTYTHLTPNELVMIEVYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGPAERTFS
                     CS"
     gene            947..1840
                     /locus_tag="IV228_002476"
     CDS             947..1840
                     /locus_tag="IV228_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8718328.1"
                     /translation="MGKISLGIDIGGTFIKYALIDKQHRIKEKWKVETVKYNTADEFY
                     DYICSNIRNVDEIDKIGVSAPGLIDEDSNVKSYAAPNVAIMYGTNINNEINKRTNKKV
                     SAINDAKAAGLCEFKIGNAKGYKSSAFLIIGTGTGGCICDENGVVYGKDSFAGEFHNM
                     PFVNAEIGGLDKMGDYASITGLVNLYNKKANREEQVQYGNEVCKKYLNGNESAVSSVD
                     EWIGNIVAQLIVITVFYNPEVICIGGGISEENWFINKVRETYKKTCREYLEADFITTE
                     INRCKHNNDANILGAVIKASI"
     gene            1914..3353
                     /locus_tag="IV228_002477"
     CDS             1914..3353
                     /locus_tag="IV228_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8718329.1"
                     /translation="MIYEELDKFQDDFLWGSASAAYQVEGAWDKDGKGESVWDVYTRQ
                     PGTTFKNTNGNIAVDHYNRYKEDVALMAEMGLKAYRFSIAWTRIYPNGRNEVNEEGLK
                     FYENLIDELIANNIQPIITLYHWDLPQHLQDLYGGWESREIIHDFNEYCVTLFKRFGN
                     KVKHWVSLNEQNIFVTLGYVTAMHPPAVKDQKRMLEANHIANLANATVIESFKKYVPD
                     GMIGPSFAFGPVYPFSCDPKDVLSAENAEDLNCNWWLDVYCKGKYPVFAFKYYERLGI
                     APTIEDGDLELLERVKPDFIGINYYQTSTVAFNPLDGVGQSEGINNTGKKGTTKESGL
                     PGVYKNAKNAHLETTNWDWAIDPTGLRIGLRRLTSKYNLPILITENGLGEFDKLEEDD
                     TVNDAHRIDYLSAHIKACKEAITDGVDLLGYCTWSFTDLLSWLNGYQKRYGFVYVDRD
                     ENDQKDLRRIKKNSFYWYKNVIRTNGEEL"
     gene            3401..3703
                     /locus_tag="IV228_002478"
     CDS             3401..3703
                     /locus_tag="IV228_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8718330.1"
                     /translation="MKKILLVCSAGMSTSLLVTKMRNSAKEKGIEVEIDALPVAEAST
                     AVDNVDIVLLGPQVRFQKGTVEKLVKGRIPVEVMDMRLYGMMNGKAILEDALQKIN"
     gene            3722..5086
                     /locus_tag="IV228_002479"
     CDS             3722..5086
                     /locus_tag="IV228_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8718331.1"
                     /translation="MSFMDKFQSAIERLLVPLSTKLNAQRHICAVRDAFILSFPLTMA
                     GSLMVLLNNVFLSADGFMFKILQFDKLFPGIVKFQAVFAPVVKGSADIFSILIVFLIA
                     RNLAKLMEGDDLLTGLTAISVYFIIYPDYFDNEGVNYLTNQYMGAQGLFVAILVGLLV
                     GELMSRLAKSDKLEIKMPEQVPPAVARSFKVLIPIIITTVFFSVLNYFVKMAAPGGLH
                     ELIYNIIQTPLTRMSQSLFSVIILALLSQLLWAMGIHGPNTIAAIRDTMFSEAGNANL
                     LHYAESGTTWGAPFPITYSGLATAFAEYGGSGATLGLIIAILIFSKNKESKSIGKLSF
                     VPGLFNINEMVIFGLPIILNPIYIIPFIIAPLVNIMIGYTAVMILKIMPPVTIGIPWT
                     TPGPLMPFLGTGGNIVALFVGFICLAVSVLIYSPFVIAANKAAIIEDEMENEIQSQEV
                     VESI"
     gene            5142..5456
                     /locus_tag="IV228_002480"
     CDS             5142..5456
                     /locus_tag="IV228_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8718332.1"
                     /translation="MEGIELTAFSIISNVGMAKSLAIEALRDARKGKYNEAEKKMAEA
                     SEYFVKGHHAHTSLIQKEAAGEKVELSLIIMHAEDQLMSTETTKLLIEEMIEMFKQLN
                     NK"
     gene            5790..6836
                     /locus_tag="IV228_002481"
     CDS             5790..6836
                     /locus_tag="IV228_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8718333.1"
                     /translation="MMNSTEIAKIAGVSRSTVSRVINNYSNVSEETREKVLRVIKEHE
                     YVPHASARMLAGSQNKVIGLFIIDLIHEEVGLKNRITKSPYYLEFTSSVIETASEMDY
                     LVLVHIIHSVAGYEKIKESFYNKTISGGIFIGQNDDDESIKTIIDRGYKVVLLDQSIR
                     PEDATYNQCMIVNADNYNGAYCATKYLIDKKHKQIAHITGGTVKFSSKDRIRGYKKAL
                     EDAGIEVNKNLIVNSEFVENAGYEAAKKLFNGNSEFSAIFASNDKIAFGAIKAIQERG
                     LKVPDDISVIGFDDIESSKYFNPPLTSMKMKLGEMADIATKSIVASIEGETKPSINVV
                     PVSLVERESCGILQ"
     gene            6818..7054
                     /locus_tag="IV228_002482"
     CDS             6818..7054
                     /locus_tag="IV228_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718334.1"
                     /translation="MWDFAVAKLAELVGADYLIILIEVDNVCVYVDLKSKVQRPKMLC
                     TLNLSKNRNISQTSPSLSNYTKIMQTFFFHDKII"
     gene            7448..7945
                     /locus_tag="IV228_002483"
     CDS             7448..7945
                     /locus_tag="IV228_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718335.1"
                     /translation="MKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINSYEQLI
                     VDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIKKNGDIKVNIFPI
                     PFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILLPSSLK
                     SYFRL"
     gene            7988..8139
                     /locus_tag="IV228_002484"
                     /pseudo
     CDS             7988..8139
                     /locus_tag="IV228_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2815 a         1267 c         1692 g         2365 t
ORIGIN      
        1 tgaacaagag aaggttcgct ctgcagggcc aataatgacg tctggggtcc aaccttcagt
       61 tgatcttttt gcaatgtatt ctttttcctc agcaggaaaa atgatttcgg ttctcccaca
      121 acgtcgctta ttttctttgt attgttcaaa gtattcaagt gctgttccac cacatttgag
      181 aaagttatag acattgtaaa ttgtttggcg tccacgttta agctgcttcg caacgatggc
      241 aaccggagtt tcttgatgaa aatatacttc tatcattaca agctcgtttg gtgtaagatg
      301 ggtataagtc atttatgttc actctccttg tatgctttag cgggtattac gatttgagtg
      361 taacataaat ggctttttta tttgtctcgc ttaattatac aaacggcgtc attagaaaat
      421 gatggtaaat tatttgatgg taacggtgat gtaacaacgg taatgaaatt attgctttaa
      481 cagtttagcg tgataaaaat ctataccttc ttaagttgat ttattaactt gagaaggtat
      541 ttttttctga aaaatgataa tgaaaatcaa aatgttcatt tctatcaatt ttgaagtcaa
      601 aaaaataaag tacagcccag aaatttgctc tgtactttaa aaaactagtt aaatgaagat
      661 gaaatcgctc ccttattttt tagtattggt ggaattaaaa ggtacttttt aaacaaaaac
      721 tacacataaa aaataaaaaa aataaaaaaa gtattgaaat gtaattgctt tcagtatatg
      781 ctatatctat aaacgcgcgt tcatgggatg ttcatcgtat aaaaaaaggt ggaaatgagc
      841 gtatcgtttt tctttttaac aaatcatgaa cgcgcgttta tgagtttgaa tcaagctgca
      901 atataagaag gcaaactgca agaccttcat tttaaggaga agtgttatgg ggaaaataag
      961 tttaggaata gacataggag ggacgttcat taagtatgca ttaatagaca agcaacatcg
     1021 catcaaagag aagtggaaag tcgaaacggt taagtacaat acagccgacg aattttacga
     1081 ttatatatgt tcaaatataa gaaatgtaga tgaaattgac aaaatcggtg taagcgcacc
     1141 cggccttatt gatgaagatt cgaatgttaa atcctatgct gctcctaacg tagctataat
     1201 gtacggcaca aatatcaata atgagatcaa caaaaggaca aataaaaaag tgtccgcaat
     1261 caatgatgca aaagcggcag gtttatgtga gtttaaaatt ggaaatgcaa agggatacaa
     1321 atcttcagcg tttttgatta taggtacagg aacaggcgga tgcatatgcg acgaaaacgg
     1381 agttgtgtat ggaaaagatt cgtttgcggg ggaattccat aatatgcctt ttgtaaacgc
     1441 tgagataggc ggccttgata agatgggtga ttatgcttcg ataaccggat tggttaattt
     1501 atacaataaa aaagctaatc gggaggagca ggttcagtat ggaaatgagg tttgcaagaa
     1561 gtacctaaac ggaaatgaat cagctgtgtc ttcagtagat gaatggatag gaaacatagt
     1621 ggctcaatta atagtaatca ccgtttttta taatcctgaa gtgatatgca tcggtggagg
     1681 aatatccgaa gaaaactggt tcatcaataa agtaagagaa acgtataaga aaacctgtag
     1741 ggagtattta gaagcggatt ttattactac cgaaatcaat agatgcaagc ataataacga
     1801 tgccaacata ctgggtgctg ttataaaagc ttctatctaa aatacacaac agttttgttt
     1861 caagaaagaa tgtttgaaaa taaatttata ttgaaaacga aggagaagat tcagtgatat
     1921 acgaagaatt agacaaattc caagatgatt tcctatgggg atcggcatct gcagcgtatc
     1981 aggttgaagg agcatgggac aaagatggaa agggggaaag tgtatgggat gtgtacaccc
     2041 gccaaccggg gacaacattt aagaatacaa acgggaatat cgcggtcgac cattacaata
     2101 gatacaaaga agacgtagct ttaatggctg aaatgggatt gaaagcttat agattctcaa
     2161 ttgcatggac acgtatttat cccaatggaa gaaatgaagt aaatgaagaa ggcttaaaat
     2221 tttatgaaaa cttgatagat gaacttatcg cgaacaatat ccaaccaatc attacgttgt
     2281 accattggga tttacctcaa catcttcaag atttgtacgg cggatgggaa tcaagagaaa
     2341 tcattcatga ttttaatgaa tactgtgtaa cgttattcaa gagatttggg aataaagtta
     2401 agcactgggt atcattgaat gaacaaaata tatttgtaac actaggttac gtaaccgcta
     2461 tgcacccgcc agctgttaaa gatcaaaaga gaatgcttga ggcgaatcat atagcgaact
     2521 tagctaatgc taccgtaatt gaatccttca aaaaatatgt tccagacggt atgattggac
     2581 caagctttgc atttggacca gtttatccat tcagttgcga tccaaaagat gttttatccg
     2641 cagaaaatgc agaggattta aattgcaatt ggtggctcga tgtttattgc aaaggtaaat
     2701 atccagtgtt cgcttttaaa tattacgaaa gacttgggat cgcacccact attgaagatg
     2761 gagatcttga attgttggaa agagtaaaac cagattttat cggaataaat tattaccaaa
     2821 caagcacagt tgcattcaat ccgcttgatg gagtagggca atcggaaggt atcaataaca
     2881 caggtaaaaa aggcaccact aaagaaagcg gattacccgg agtttataaa aatgcaaaaa
     2941 atgcacatct ggaaacaaca aattgggact gggcaattga tcctactggt ttaagaattg
     3001 gactaagaag attgaccagc aaatataatc ttccaatact tattacagaa aacgggcttg
     3061 gagaattcga caagcttgaa gaagacgaca ctgtcaatga tgctcacaga atcgattact
     3121 tgagcgcaca tatcaaggct tgtaaagaag caataacaga tggtgttgat cttctaggct
     3181 actgcacgtg gtcatttaca gatttgttaa gctggttgaa tggttatcaa aaacgctatg
     3241 gatttgtgta tgtggacaga gatgaaaatg atcaaaaaga tttgagaaga atcaaaaaga
     3301 acagcttcta ttggtacaaa aatgttattc gcaccaatgg agaagagtta taagaatcgt
     3361 tcacaatcaa tgaatatgaa aaaacaagga gagatagacc atgaaaaaaa ttttactagt
     3421 atgttcagca ggaatgtcca caagtttgct tgtgaccaaa atgagaaact ccgcaaagga
     3481 aaaaggaatt gaagtagaaa tagatgctct tccagttgca gaggcaagta cggccgttga
     3541 taatgtcgat atcgttcttt taggaccaca agtgcgtttc cagaaaggga ccgttgaaaa
     3601 acttgtaaaa ggcagaattc ccgtagaagt gatggacatg cggctgtatg gaatgatgaa
     3661 tggaaaagcg attttagaag atgctttaca aaaaataaac taggaaagga ggctaaaaaa
     3721 aatgagtttt atggacaaat ttcaaagtgc tatagaaaga ttgttagtgc ctttatctac
     3781 aaagttgaac gctcaaagac acatatgcgc agtaagagat gcttttattt tatcattccc
     3841 attaacgatg gcaggatcat tgatggtgtt attgaataat gttttcttgt cagcagatgg
     3901 attcatgttt aaaatcttgc aattcgataa actttttccg ggtattgtta aattccaagc
     3961 agtgttcgct ccagttgtta aaggttcagc tgatattttc tcgattctga ttgtgttttt
     4021 gatagccaga aaccttgcga aactaatgga aggcgatgat ttgttgacag gtttgaccgc
     4081 aatctctgtt tatttcatca tctatcctga ttatttcgat aatgaaggag taaattatct
     4141 aacaaatcaa tatatgggcg cacaagggtt gtttgtagca attttggttg ggctcttggt
     4201 cggcgaacta atgtctagat tagcaaaatc agataagtta gaaattaaaa tgccagaaca
     4261 agtacctcca gcagttgcaa gatccttcaa agtgttgatc ccgattatca tcacgacggt
     4321 tttcttctcc gtcctcaact actttgtaaa aatggctgca ccgggcggat tacatgaatt
     4381 aatttataac atcattcaaa caccattgac cagaatgagt caaagtttat tctcagtgat
     4441 tattttagca ttattatctc aattgctatg ggctatggga atacacggac caaatacgat
     4501 tgcagctatt cgagatacca tgttctcgga agccggaaac gcaaacctct tacattatgc
     4561 tgaaagcggt actacatggg gagcgccttt tccgataacg tacagtggtt tagctactgc
     4621 tttcgctgaa tatggcggtt caggagcgac attgggtcta atcattgcta tattgatttt
     4681 ctcgaaaaat aaggaatcga aaagtattgg caaactttct tttgtacccg gtttgttcaa
     4741 tataaacgaa atggttatct ttggtttacc gattattctg aatccgattt acattattcc
     4801 atttattatt gcgccactgg ttaacattat gattggttat accgcagtta tgattcttaa
     4861 gattatgccg ccggtaacga ttggtattcc ttggacaaca ccaggaccat tgatgccttt
     4921 cttgggtaca ggcggaaata tcgtcgcact gtttgtcgga ttcatctgtc ttgcggtgag
     4981 tgtattgatt tactcgccgt ttgtaatcgc agcgaataaa gcagcgatta ttgaagatga
     5041 aatggagaat gaaatacagt cacaagaagt agtagaatcg atttaaaaaa gctgcagtac
     5101 gctgagaaca aaccgaaaga ttcaagatag gagatttaca catggaagga attgaattaa
     5161 ctgcttttag tataatcagt aatgttggaa tggccaaatc attggctatt gaagcattaa
     5221 gagatgccag aaaaggaaag tacaatgaag cagaaaagaa aatggctgaa gcatcggaat
     5281 attttgtgaa aggccatcat gcacatactt ctttgataca aaaagaagct gctggcgaaa
     5341 aagttgaact ttcgttaata atcatgcacg cagaagatca attgatgtcg acggagacaa
     5401 ctaaattatt aatcgaagaa atgatagaaa tgttcaaaca gttaaacaat aaataagtga
     5461 acatgaaaga gaaaatatta gacgaactag aattgcttgc ttactttaca ggcagaatct
     5521 gatgccagca gcaaaacata gagcacccag gatgaaatat aatcctgggt gccttatttt
     5581 tagaacaaaa caccggggtg gtagtttgtt ctgcggttga aggcatttgc tgatggtttc
     5641 tgcatgaaag gtattgccaa aagtctcctt ttgctggaat cctgaagcag gcagagtttt
     5701 tcgaaaaaca gaaaaagatt gaaaaaaatg gacaaataat atagtgttgc agtatgaaca
     5761 cgagtttacg aacgaaagga gatgccttta tgatgaatag tactgaaatt gcaaaaattg
     5821 ccggagtatc acgcagtact gtatccagag tgataaataa ttattcaaat gtctcggaag
     5881 aaacaagaga aaaggtttta agagtaatta aggaacatga gtatgtgcca catgcttcag
     5941 caaggatgct tgcaggaagc caaaataagg tgatcggctt atttattata gacctgatac
     6001 atgaagaagt tggactaaaa aatagaatta ccaaaagtcc gtattatcta gaatttacta
     6061 gttcagtgat agaaacagca agtgagatgg attacctggt tcttgtccat attatacata
     6121 gcgtcgcagg ctatgaaaaa attaaagaga gtttttataa taagacaatt tctggtggga
     6181 ttttcatagg ccagaatgac gacgatgaat cgataaaaac aatcattgat agagggtata
     6241 aagttgtgct gctcgatcaa tcaatcagac ctgaagatgc cacctataac cagtgcatga
     6301 tagtgaatgc ggacaattac aacggcgctt attgtgcgac aaaatatctc atagataaaa
     6361 aacataaaca aatcgcacat attacaggcg gtacggtcaa attctcttct aaagacagga
     6421 taagaggata caaaaaagca ttagaagatg cagggataga agtaaataag aacttgattg
     6481 taaatagcga atttgtggaa aatgctggat atgaggctgc gaaaaaatta tttaatggaa
     6541 atagtgagtt ttcggctatt tttgccagta atgataagat agcatttgga gcaatcaaag
     6601 ctattcaaga aagaggtctc aaagttccag atgacatctc tgttatagga tttgatgata
     6661 ttgaatcgtc taaatatttt aatccgccat tgacttctat gaaaatgaag ttaggagaaa
     6721 tggctgatat cgccacaaag tcgattgttg catcgattga aggtgaaacg aagccatcta
     6781 taaatgtagt acctgtctca ttagtggaaa gggagagttg tgggattttg cagtagcaaa
     6841 acttgctgaa ttagttggag ctgattattt gattattttg attgaggtag acaatgtctg
     6901 tgtctatgtc gacttgaaaa gtaaagtaca gcgcccaaaa atgctctgta ctttaaatct
     6961 cagcaaaaac aggaatattt cccaaacatc tccttctttg agtaattata caaaaattat
     7021 gcagactttc ttttttcatg ataagataat ttaaaaagtt tttaaacgat ttctcctcct
     7081 ctttttctta tatcggggga gcatattttt ttgtagacta ggggattata agttcagcca
     7141 tcaatgtcta gctcccaagt cctgacctag tgaaaaaaag ataaatttgc cccattgcgc
     7201 gcttcgctgc tcattcaggg tcaaatttcc tattttttca taggtcaagg cggacttgtc
     7261 cgcttttctt atattaagtt tacaaaggat gcgaatcacg cagagaaagt taaaagtctt
     7321 tcttctaata cttttggttg gggtataaag ttatttcttt tgaaactctt tgaacaaggg
     7381 attgcaaaaa ttgaagcaac ttttgtcgaa gctcaaaaaa atatgggctg ttttacctga
     7441 acattctttg aagcaacgca ttggtaataa tctagctttg atttatagtg gagctctcct
     7501 aattcaagat atactgggct atcctttacg attagagcat atacagacgg tgcttattaa
     7561 ttcgtacgaa cagttaatcg tagatactag tttaggaagt gagcaaacgg tatttgaccg
     7621 agtgaaaaca ttgttggtga tgaatagcca taaattttta acaaacagtg acaacaaaca
     7681 tacgcccagt acgatttggg gaaaagtatc aattaaaaaa aatggggata ttaaggtaaa
     7741 tatttttcct atcccgtttg aggacttgtt aaggcgagaa tttcaagtaa aggatctaac
     7801 ctatcttttt agacaattgc ttgattcggg taatatgtgt gcagaaaaag ggagaagaac
     7861 gaaacgtatt cacatgaatc gtcaagttct tccaacatac gaaattctat taccttcttc
     7921 actcaaatcg tattttcgat tgtaatttag aataaaccaa agtatgaaag tgaggcacca
     7981 tgttattaag caactgaaag atattcctat tccaatgccc gctatttcag aacaacaaaa
     8041 aatcggcact ttcttcaaac aactcgacga caccattacc cttcaccaac gtaagttaga
     8101 tttactaaaa gaaacgaaaa aaggcttctt acaaaagat
//
LOCUS       DACDHL010000107         8055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDHL010000107 DACDHL010000000
VERSION     DACDHL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.110"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            125..3652
                     /locus_tag="IV228_002485"
     CDS             125..3652
                     /locus_tag="IV228_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8718336.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3649..7371
                     /gene="addA"
                     /locus_tag="IV228_002486"
     CDS             3649..7371
                     /gene="addA"
                     /locus_tag="IV228_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8718337.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7440..7781
                     /locus_tag="IV228_002487"
     CDS             7440..7781
                     /locus_tag="IV228_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8718338.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
BASE COUNT         2780 a         1378 c         1703 g         2194 t
ORIGIN      
        1 tatacttaaa aaagtagaaa tatcagaacc ttaaaaggat ataatttgaa gaatatagaa
       61 caaatgttca gtattttttg atataataaa ggagcaccgt atcatgtgtt gaaggaggaa
      121 aagcatgtct ttacaattta ttcttggaaa cgggaaaaaa gatcaccgtc aagtcttgat
      181 cgacgaagca tccgcttggc tcgaccagga tgcaagtaac caagtatttt tccttgttcc
      241 caactacagc aaattcgaac aggaacaaga aattttagcg gagatgcgaa gaagaagcgg
      301 aaaaaaaact ttcagcacaa caaacgttca agtatttagt ttttatcgtc tggcttggta
      361 tttgctgcaa cagacaactc ttcttacagg aaatgaatta tcagaatcag gatctgcaat
      421 gatcttgcga cagattttag aaaaaaatga ggaagaatta acaatctttc gcggagaaat
      481 caataaaaga gggtttattc gtcaattaca agaactctac agtgaattgc gtacaggaaa
      541 tattcaacca gaagacctga tgcttttatt tggtgattca cctaatgcag aagaccagca
      601 acttaaaatg aaagacttga agctgatttt ttcagcttat gacagcgaat tgactcaacg
      661 tgcactaaca aatgaagatg cactatctat actagcaaat tatatgcaga cacaagattt
      721 caacaacgtc aaatttattg tcagtggttt ttctcgaatc aatggtcaag agtatcaatt
      781 actgcaggca atgatggaaa aggggcaatt ggttgtcgat ttattgctgg atcgtcctta
      841 tacagcggat cttccagaag tacttacgct ctttcatgag actggaaagc tttatttccg
      901 tctttttcaa agtgcaagag aacggaaagt ccctatatta gtggaccgat ttgcaccaga
      961 gcagactcgt ccgacagagc ttcaactgct gcaaacaatg tgggaagaga catctagcca
     1021 aaagaaagcg gcagtacaca aaataggggg acaagagcat ctccatttgt gggaagctga
     1081 aaatccaact gaagaagtcc gacaattagc tgttgaaatc agacgactag tcagtgaaga
     1141 aaactaccgt tatcgtgata tccaagtgct gacaaaaaat atgtctttat atggaaatat
     1201 ccttcggccg atctttcaag aaatggcaat tcctttttat atcgatgaag aacaaatgat
     1261 ggaaaatcat ccgctgatcg aatggatcaa cagtctgttc gctcttgacc gctattctta
     1321 tcggctaagt gatattatgc gttttttcaa aactgaatta tttctgtcga cggaagcaga
     1381 aaccgaactt gatgaatgga aacaagagcg agatgaattc aggaaaaaac tggacatcac
     1441 tgaaaatgta gcacttgctt acaattatca aggaacttat tggactcgtg aaaaagactg
     1501 gcagtttgtg gcgtatgatt ttgaagcaga acagattgaa gacgtacagt atttggaaga
     1561 acagtcaaat acgattagac gattatttca gaaagctgta ccagtatttt tgaaaaaaat
     1621 aaagaaaagc aaaacaggac aagaggccgc aaatgttttt tatcaatttt tgatccaaag
     1681 tggcgtacag caacaattat tgttttggcg taatcaggaa gtagacagag gaaacttaga
     1741 agcggcaaga aaccatgagc agacatgggg agcgctgatt gatctgttgg atgaatatgt
     1801 agtaatttat ggtgaatcgg cttttgactg gtcactgttc caagaaatca ttacaagtgg
     1861 tctagaaaat ctaagttatg gtaaaatccc taccgcaatc gaccaagtgc gaatcaatcg
     1921 tttggaatta gttcgaccaa atcaagctgc cgtcactttt gcattagggt tgaatgatca
     1981 agtgtttcca gaccgaaaag agacaaaagg attgttgtcc agtgaagaac gagaagtatt
     2041 aaatcaggcc ttgccagatg gacagttttt attcgaccca tcaaaggaaa gtatctcttt
     2101 tgagcctttc caagcctatc ttgtttttct ttccggaaca gatcgattat atcttagtta
     2161 tgcacaaagc tatgacacag atggttcgct gaagatgtct ccttatttgc gtagaatcac
     2221 agaatattta tctgttcccg tcgagagaaa agagcatttg acgatcgaaa gcgaccctaa
     2281 ttgttatgta gggacttaca gaagcggaat caatacagtc aataggattt atcgattagc
     2341 tttagatgaa aaaaaacctg taccaaagca ctggcaaaag ctcaaagaat tagtactgaa
     2401 ttcgtcatgg cgaaattttg ctttacagat ttttgaaagt caggaacatt taaatgttcc
     2461 tgtttcgatg ccagaaaaaa tggcggaaga gctttatgga aaagagattt atacttcaat
     2521 ttcacgtatg gagaattttt ataattgtga atacaaatac ttcatgcaat ttggtttgaa
     2581 attgaaagaa cgaactgttt atggtctaac tccagcagca actggtgatt tttatcatga
     2641 atcattggat cgtttcttca agctactgtt ttcaaaccaa ttatccttag tgaatatgtc
     2701 cgatgcgcag cgtagagact ttactgaaaa agtgctgcag gaagtattcg gagaacttcg
     2761 gtttgagata ttggatagtt ctgcaagaat gaactatatc cgctaccagt taggacagac
     2821 gattcaaaaa gtgacatggg ctttgcaaaa acagagcaaa aatacaggca tgcatcctct
     2881 tcagacagag attctatttg gtcagattgc tggagcaaaa ggtatcacag gtctggaatt
     2941 accgttgagt aatggtggaa aactccatgt tcgagggaaa atcgatcgaa tcgatgtagc
     3001 ttctgaacaa gaagatactt ggctaagcgt cgttgattac aaatctagcg gacgttcatt
     3061 cgatgtcaca gaagcttatt atggcatggc tatgcaatta ttgacgtatc tggacgtagc
     3121 attgatggat gcggtacgtt tgactggaaa ggcggctgtt aaaccagcag gatcatatta
     3181 tttacatgtt cataatcctg tattgacgga gacagatgat gtagagaaga atacattgaa
     3241 aaaatttagt tatgatggcg tttttgtcga tgatcccgct cttttagaag tactagatca
     3301 ttcactggaa gcaaaagaac aatcactgat ctatccagtg aaaaaaaatg cgaaagatga
     3361 ataccaaaaa ggtagtttca gtaaagacaa atttttcacg gaagaagaac tgcaactatt
     3421 catggcgcat aatcgggaaa atatgcggtt agctggagat gcgattactt ccggagaaat
     3481 caaactaaat ccagcttata aagataaaca gcggattgcc tgcgaatttt gtccattcag
     3541 aagcatctgt acgtttgatg tgatgctgaa agaaaataat taccaccgtc tggagaaaat
     3601 agataaggaa gaagcactgc gtagaatatt gaaaaggagt gaagaagagt gaatcaaatt
     3661 cctttaaaac cgcaaaatga acgattcaca gatgatcagt ggcaagcgat ttttgatcaa
     3721 ggagataatc tattagtctc tgcgtctgcc ggatcaggta aaactacagt gcttgttcga
     3781 agagtgattg aaaaactaaa aatgggtttt gatatcgatg aattattgat tgtgacattt
     3841 actgaagctg ccgcaagaga aatgaaagaa agaattcaag aggcattaca agaatccgtc
     3901 aatagcgaaa gtgaccctgt acgtagacag cattttacta agcagttagt tttattgccc
     3961 acagcgaata tctcaacctt acatgctttt tgcctgacag ttattcgacg ttattattac
     4021 ttaattgata ttgatccagt ctttcgcatg ttaacagatg aaacagaaac gatcttgatg
     4081 aaggaggatg tgtgggacga gctgagagaa gcactttatg cagaaaatga tgaacgattt
     4141 ttccaattaa caatgaattt ttctaatgat cgttcagatg acggcttgac gaatctggtg
     4201 ttttctcttt atgagtttgc ccgtgccaac cctgaccccc aaaaatggct agaacagtta
     4261 agcgataatt atcgtttgcc tgaggggcta gcgaaatctc gattatatca aaaacaaatc
     4321 cgtcctttag ttttggcaga tatataccaa tgtgtacagt tatatgaaca gatgactcag
     4381 ttagcacaag gtgaaggatt agaaaaaatg catgagcaag tagctggaga acaacagcag
     4441 atcaagaata tctatgaagc gttctcgcaa gatcggttag aagaagctta tgctggtttg
     4501 gaacaactaa ctttttcaac ttttaagagc agtcggaaag cagaattgaa agaaatttcc
     4561 aatgaagtca agggcatgag ggataaagca aaaaaactga ttcagcagat aagtaaaagt
     4621 tattttcctg tttccccaag ccaaatggaa gaattaacgg acaaagcact accgttagtc
     4681 gaagaaatga caaaagtcac gcaaagcttc atggacggtt tcagtatgcg taaacgggaa
     4741 aaaggtgtgc tggatttcaa tgatctggaa catttagcgt tgcagattct cacagaaaaa
     4801 acgaaagatg tatggctgcc aagtgaagct tcaaaacatt atcgaaaaaa attcaaagaa
     4861 gtaatggtag atgaatatca agacgtgaac caattacaag aagccatttt atactggcta
     4921 agagaacccg atgatacaaa aggaaatatg ttcatggttg gggatgtcaa acaatccatc
     4981 tattctttcc gtttagcaga tcctagttta tttatcggga aatacgagaa tttctcaaag
     5041 aaagaaggcg gacgtcgaat cgttttggct gagaacttcc gctctagaaa agaagtcctt
     5101 tcttttacga atcttatttt tgaacaacta atggatccag cagtgggtca aatcaattat
     5161 gacgaagcag ccaaactgat ccaaggattt tcagactttc cagagaatga acagtttgag
     5221 ccggaaatca tgatctatga aaaagaacag gaagaatcag agattgagat tccgacggat
     5281 gatatactgg aagacaaaac agaaggcgaa ctttttatga caggactaaa aattcgtcaa
     5341 ttaattgatt cctcctttat gatttatgat aaaaaatcaa aaaaaagtcg tccgatagag
     5401 tacaaagaca ttgttctctt gacacctaca aagaagaaca acttaacgat tttagagatt
     5461 ttcaaaacat tggatattcc attagagatg aatgatgctc aaaactattt tcaagcgaca
     5521 gaaattcgca cgatgatctc gctgctacaa ttaatcgata atccctatca agatattccg
     5581 cttgcggctg tattgcgctc accgatcgtg ggtctgattg aaccagaatt agccagcatt
     5641 cgattagcta accgtgcaca tacttactat gatgctgtgc ttgcttatca agcatcaaac
     5701 gaagatgaat tagcagctaa gctggaacat tttggcaagc aactagagca ttggcgagaa
     5761 cttgctcgcc gttcttcgat cactgacttg ttatgggata tctattatga gacgggatat
     5821 ctagagtatg tggtcggtct gccagcaggg gcacagagac aagccaatct ttatgcattg
     5881 gttgatcgtg ccaaagctta cgagcaaagt tctttcagag gattatacca gttcgtgcgt
     5941 ttcattgaaa aaatgcaaga aaaagataaa gacttagcag aaccagtcat ttcaatagaa
     6001 gataatgctg ttcgcgttat gacgatccat gcaagtaaag gcttggaatt cccagttgta
     6061 tttttgttag atatgacaaa ggagttcaac ttacaagact tgcgcaatcg gtatgctttt
     6121 gaagaaaaac ttggagcagg aattcgctat atggatcctg aaacacgagt gttatatgat
     6181 acgttaccgt ttcaagccat caaattggca aagcaaaaca aattgttgtc ggaagaaatg
     6241 cgtaaattgt atgtaggatt gactcgggca gaacaaaagc ttttcattgt cggttcctat
     6301 aaaaataaag agcaaatgat ccaaacgtgg tctgaagcag ctgatcatga ggagttagtt
     6361 tttgatcctg cgctacgttt gaagggcaga agcagcttga tgaattggat tggttacgga
     6421 ttgatccgtc atcctgaaat gcagaaatac ttagaggaag aaatttcgac ttctcttctt
     6481 caacattcaa atgcacagtt ttctatttca tggatgaatc agcaatcgat cattgaacaa
     6541 agacagttgc ttgctgaaaa agaactggtt aacttagatc agcagatgaa agaagatgaa
     6601 actttattag cagattcgtt acaaaaacgt ctggcatatg aatatcctta tcaagcttct
     6661 tcacaaacca caagttatca atctgtatct gaaatcaaac gattattcga agatccagat
     6721 gatacacaag aatcacgttt gactctggaa agcagtcaga acaaagcagc atcccgtcaa
     6781 tttcgttata cgcaagaaca actagcagaa ccaaaatttt tgcaaaaaga tcggcaagtg
     6841 tcagcagctg ctgttggaac tgctacgcat gctttgttac aattactgcc attagagatg
     6901 ccaactacag agagtatcca tcaaaaatta caggaattgg taaggaaacg gttagtagac
     6961 gaaaaagtcg cgaaaaaagt agatgtttct tctattatat ggttctttca aacagaactt
     7021 ggtcaacagc tgattgccaa caaagaaaac gtaaaacgag aacaaccttt ttccatgctg
     7081 cttcctgctg acgaagtatt tcaagactat ccaaatcaag aagatgaact attaatccac
     7141 ggaatcgtcg atggatacct agaagaaaaa gatcatctca atatctatga ttttaaaaca
     7201 gattttattt tacatccgga tgatccagca gaaatagatg caatcgtcca aaaatatcaa
     7261 ggacagctaa gactttacca acaagcaatg tcagaagctt tgaataagcc agtagaaaat
     7321 gttttcctaa ttttgcttcg agtgaaacaa attataaaca ttaacaaata attataattg
     7381 agcaaacttg acttatgttt tgtaaggatt atgctatact ctgggaagag gtgagtatga
     7441 tggagctaac acagacagaa ttcgttttat gccctaaatt tgaaaaaaca ttctctattt
     7501 taggaaaaaa atggaacgga ttgattatcg atgtactact cgaaaacggt ccgcaacgct
     7561 ttgttgagct tgcggcaaaa atcccgatgg tcagtgatcg agtattagtc gaacgcttga
     7621 aggaattaga aaaagaagga atagtcactc gaaactttgc ctgtagcgaa aagagccgtt
     7681 cagaatatct cttgactgaa aaaggagcag acttgcaaaa agcgatgcgt caaatacaag
     7741 tttggagcga aaaatggatc acagaggaag aatgcggttg attttgtaaa aaaaatcttg
     7801 taaactatag tccatacgaa taagtaaaga acgatgacga aaaagagtag tttggctaaa
     7861 ccagttcagg gagatctgtc gcagactgaa agcagatcct gtgtttgaaa aatgaagttc
     7921 actttccgag ttcgtctgat aaaatcagac cggcaattgt cgttaacgaa gtaagagtgc
     7981 ggtgtaagtc atttatccgc aaattaggat ggtaacgcga agacctcgtt ccttaacagg
     8041 aatgaggtct ttttg
//
LOCUS       DACDHL010000108         7737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDHL010000108 DACDHL010000000
VERSION     DACDHL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.111"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..843
                     /locus_tag="IV228_002488"
     CDS             <1..843
                     /locus_tag="IV228_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8718339.1"
                     /translation="ETSPYSVFKWGDTAGQGLSYVAAKANIKVTNKDGSVVLKENTDY
                     KIQNSENGFVIDFIVNNGQVSDTVASLHGQDLQMTYNMYVNDSAAVANPLTNSVDFVY
                     NNNPFNQEEHHEKTKADVVTYGAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYG
                     GLVDTDKDGVKEAVWVDDVANAAILKSDKEGHFEITGLTEGEYSLEETKAPENYQKLT
                     KEISFKVDKDSYKEENRITIKNNQKASVPMTGSNGFQTYVLISCLLLGAGALSAVVYF
                     KKKA"
     gene            858..1610
                     /locus_tag="IV228_002489"
     CDS             858..1610
                     /locus_tag="IV228_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8718340.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            1626..2381
                     /locus_tag="IV228_002490"
     CDS             1626..2381
                     /locus_tag="IV228_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718341.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            2394..2654
                     /locus_tag="IV228_002491"
     CDS             2394..2654
                     /locus_tag="IV228_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718342.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            2651..4741
                     /locus_tag="IV228_002492"
     CDS             2651..4741
                     /locus_tag="IV228_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8718343.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            4768..5232
                     /locus_tag="IV228_002493"
     CDS             4768..5232
                     /locus_tag="IV228_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718344.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            5229..5471
                     /locus_tag="IV228_002494"
     CDS             5229..5471
                     /locus_tag="IV228_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718345.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            5489..5791
                     /locus_tag="IV228_002495"
     CDS             5489..5791
                     /locus_tag="IV228_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718346.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            5861..>7737
                     /locus_tag="IV228_002496"
     CDS             5861..>7737
                     /locus_tag="IV228_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718347.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     A"
BASE COUNT         2926 a         1223 c         1559 g         2029 t
ORIGIN      
        1 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaggg actttcttat
       61 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
      121 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
      181 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
      241 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
      301 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
      361 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
      421 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
      481 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
      541 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
      601 ggtgaatatt cactggaaga aacaaaagcg cctgaaaact atcaaaaatt aacgaaagag
      661 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaac
      721 aatcaaaaag cttccgtacc aatgaccggt agcaacggtt ttcaaacgta tgtattaatc
      781 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
      841 tagaaggatc aaaaccaatg attatcagga agcctaagaa aaagcgaatc atgggtaaat
      901 ggatcattgc tttctggtta ctttcagctg taggtgtctt acttttgatg cctgctgaag
      961 cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg
     1021 cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta
     1081 ttcttacaat tctaagtatt tctttaaaac taccaattta tgacggaacg tccgacaaaa
     1141 ttttagaaaa tggtgtaggt attacggaag gaactggtga tattacagga ggaaacggta
     1201 aaaatccttt aatcgcaggt catagtggac tttataaaga taatcttttt gatgatttac
     1261 cttcagtaaa aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc
     1321 aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact
     1381 tagaatcaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca
     1441 acacacaccg ttttctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag
     1501 atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc
     1561 tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa
     1621 ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg
     1681 cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa
     1741 tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag
     1801 ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc
     1861 acgttcaaaa gattatttct tcagaacaat tagacaacca ggggcatgaa aaacaagaag
     1921 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
     1981 ttaaggatct cactactgac caaattgaat cccaattaaa aaatcgagtg aaaaaactat
     2041 ccagtgatca attgaagcta gtttctaatg aagaaacaaa gacaattgat caacaaacag
     2101 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
     2161 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
     2221 ctgataaaaa cggtgaattc ttaaaggatg tgtggatcta tcctaaatca gaagcttcgc
     2281 aacctaaaga agaagtaaaa aaaatcgttt ctactggcgt aaagaaaaac ttctttgaga
     2341 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
     2401 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
     2461 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
     2521 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
     2581 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
     2641 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
     2701 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
     2761 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
     2821 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
     2881 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
     2941 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
     3001 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
     3061 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
     3121 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
     3181 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
     3241 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
     3301 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
     3361 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
     3421 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
     3481 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
     3541 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
     3601 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
     3661 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
     3721 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
     3781 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
     3841 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
     3901 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
     3961 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
     4021 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
     4081 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
     4141 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
     4201 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
     4261 acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac aatttagtat
     4321 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
     4381 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
     4441 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
     4501 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
     4561 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
     4621 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
     4681 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
     4741 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
     4801 attgccatga ttgttatttt agtcggatgt agtaatgaaa aaaataaaca aactaatcaa
     4861 gataatggcg tattgaaatc aggaactatg tggaaagaag aagtaggtgg tcttgtttat
     4921 aatttgaaaa ttatagacga gactacctgg gaatactctg agagtgtatg gcatccagat
     4981 cctgtacaaa taacagtcaa gaggcaaaaa gactataaag gtttagaaag atacaaaata
     5041 gtagattcag ctggtgttag ggaatttata aacaaatctg attctttatt tattgttgtt
     5101 ccctatgaga agaatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgaaaag
     5161 cgaacgaaag agaaattgat tcacgatgga tcacaatcca ataaatataa gttacaaaag
     5221 actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca gtggcaaaag
     5281 ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt gtatctgaaa
     5341 cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg ggaaaagaca
     5401 aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta
     5461 ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga attttttaac
     5521 acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg ccgcaactag
     5581 tttaaaaaac aatctgatga tttttgctgc tgctgcggcc gttgcttcca ttgttattgg
     5641 gggtcttttc ttcctatttg gacgaagagg agctgaaaat gggaagatgc acgtaggcta
     5701 tgtattgatg ggggtaggag ttattttagc agcagcttct attgtggttt cgttatttgg
     5761 attgtttgga caaacacctc aaggattata agaaagtaaa aaagtaggag taagaagtag
     5821 tcgatacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgattgcagg
     5881 caataccaaa acagataaag agttaataaa aaaagcaacg aaggaagtag gaaagcgtac
     5941 ttctttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
     6001 gttagggaaa gtactggaac acgaaagtcg atcttctgtt aagttgactc tcacgttaca
     6061 tgacacagaa ggaaaaatag agaaagctgg tacagaatac gtttttggcc cactggatta
     6121 tcaaatttca gaaggtttct atcctacatt gatggattta atccgccttg atgtgtttaa
     6181 agaaacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt
     6241 tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagggagtg aagtcgaagt
     6301 aaatgaagaa gtaccatggg aacgaccaat atttccaaag aaggagtcaa aagaaaaaga
     6361 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
     6421 gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
     6481 gacggccgaa gtagaagttg atccagttga agcgaatgaa gagtcaacat ggactgaagg
     6541 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaataga
     6601 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
     6661 gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca
     6721 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
     6781 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
     6841 attagccacc tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
     6901 ggaacagtcg tctaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
     6961 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
     7021 ttccttgtgt gtagcaaaga tcaataattt tactcagacg aatcaagaag cgttatcgaa
     7081 agaaaaagaa aacttgttaa aagaacgtat taaagaacga aaagaagaat tttaccgagg
     7141 tttgcgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataagctata
     7201 tcaaacaacg actgaagttc ttatggaaaa atacgaaaag atccagcaag aattacaagt
     7261 gcaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
     7321 agaaaaacaa cggcaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
     7381 ggatcttgcg gaacaacaaa tggcaataga aaaacaacgg ttaaaccaag aaaaggaaaa
     7441 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagctcagtt
     7501 aaattatcta gcacaacctc aaccaacgcc ttcagtagct gctgttcaaa ccccaatgaa
     7561 caaccaaaca aatctaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
     7621 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
     7681 tcaatcttca caggctgatt ctgtggccac aacgacgtca atcaaggaag aagccca
//
LOCUS       DACDHL010000109         7694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDHL010000109 DACDHL010000000
VERSION     DACDHL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.112"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..2378
                     /locus_tag="IV228_002497"
     CDS             51..2378
                     /locus_tag="IV228_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8718348.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2504..2833
                     /locus_tag="IV228_002498"
     CDS             2504..2833
                     /locus_tag="IV228_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8718349.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2873..3877
                     /locus_tag="IV228_002499"
     CDS             2873..3877
                     /locus_tag="IV228_002499"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8718350.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3874..5303
                     /locus_tag="IV228_002500"
                     /pseudo
     CDS             3874..5303
                     /locus_tag="IV228_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5509..6681
                     /locus_tag="IV228_002501"
     CDS             5509..6681
                     /locus_tag="IV228_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8718351.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNKAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6843..7298)
                     /locus_tag="IV228_002502"
     CDS             complement(6843..7298)
                     /locus_tag="IV228_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718352.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2602 a         1442 c         1531 g         2119 t
ORIGIN      
        1 ttagaataac gtgtaaagga ttatttgatg attttttatt gagaggaggg atgaaaatga
       61 ttaagaaaac tcaaaataaa atccaacgta tatgtcaaaa acattttgtt tctagactca
      121 aagctgtatc taaaagaaaa tacttacatc ccattatgat ttcgatcatc ttgattttta
      181 ctttcttagg gagcttatca agtatttatg taaacgttta tacagaaacg tcaaattttg
      241 ctaaccaaac gacagaggag acaaatagtc caccctattc tgaaatacag tccatagaaa
      301 gcaccaatca gtctagcaac aacgagacaa cctcaaccac atcctcgact caactgattg
      361 atccgacaga aaaagaaatc acagcagcaa aagcagcagg ggagaaagct tattcagaaa
      421 caggtagaag ccagacattt agtactgttg cggccactgc gaatttcaac ggacatacca
      481 taccagatgg atatacagtg atatatgcca aagatggagt cattgttgcg gttaacggat
      541 cgatgagtgc ggataaaact gcgttacaag gcgtaaccaa agcaatcgct tttattgata
      601 accgtccaga tatccataat ctgtttattg gctccacagt aaatggtact actatcacat
      661 tcccaaaaag tatctatatg ggggtggcta ccgctggaaa tacgatcgat tcagttacac
      721 ccaatgcgga taaaagttat tcttttacac cctcaacatg gggagggctt cctgccggag
      781 gaggtatgag tactttttca atcggtatac agttagcaaa tgatattcca ttcacggggt
      841 actcgatcaa aaatgggcag atcgttgatg gtaagggaaa tgtttcaaat tttccaacct
      901 atcaagcagc aacggttcag gtcaatgcga atcggacagg aactttttct tatacagtca
      961 atggtggagc agcaaaaatc ggaacgtacg ggactacatt atctggaatc catgtgggtg
     1021 acaaagtggt tatcactcca acagcagtaa cgggctattc ttttgcacaa acaaacggtt
     1081 ctttcacagc cctagaaagt aacgtcgata cggtgaccta tacaggcaaa caaacaccaa
     1141 tcactattaa gtatcaagac tattttggac agatgattgc tccttctaaa ataatgaatc
     1201 ttacttacgg aagtgctgca caagacctga cgactaatac gcctactatc aacggatata
     1261 cctttacagc tgtttcagcg acagaaacaa agaaccaatc agcaatgagt gttttagcta
     1321 gtttagatac aaatggaaat gtaatcgtaa aggacgcaaa tggaaaacag atttctgaag
     1381 tcatcctata ttataagaca aatgcaacag tatcaatcaa tgcaaatggc agtaagtact
     1441 atgatggtct ttctgttgta ccgatcgtta aatatgcctt taatgataaa acagaatcaa
     1501 catccctttt aaacaatgat ctcagtattg atcaaatcac atggaatcca actgatttca
     1561 aagcgatgaa tgaaaaggga gttgaagtaa ctgcgcctac tgaaattgga acctatacgt
     1621 cttggcaatt gacaaaaagc ggactagcta aactagctgc cagaacaaac tatttattta
     1681 ctatcgtgca gacatctgat gtctatacga ttaagcaaat cagcggtgcg gtagagctca
     1741 gtggcagtaa gacatatgat ggaaaagcag gtgtcccgtc aatccatgtc aagctagcag
     1801 agggtgtcac tagttcgctt actccagtac aagtggcact tagcagtatg gattacacag
     1861 cagatgcaca atcagcaaaa aatatggtta atgctggcag ctatacaatt aaactaacga
     1921 atagcggaat agacaaagtc aaagcagcaa atcccaagtt gtcatttact gatatagcca
     1981 acacatcagg aacttatacc atctataaag ctaacgcagt gattactgtc gatgacgctg
     2041 cctttaatta tgatgcccaa agtcactcga tttcggttgg aaatctccac gttaccggtg
     2101 ttgtcttagg cgaatcattg gattataccc taacagttaa cagccggaca gatattggat
     2161 cacaaatagt aacgattagt ttaggcgaag gaagtgtaaa caataattat acgattatcg
     2221 caacagatac cgctaagttg acgatcaatc cgactctctt gcttccttcg acaggtggga
     2281 taggtctact gccctttgtc tttttaggac tgatctttat tttttctggc ttcttttatt
     2341 ttatccatcg taagaaaaaa gcaggtgaac agcgatgaaa aaaacaaaaa atgtacgtgt
     2401 ggatatattt gctgttctct tttttatcct actggtcttt tttataggtt ctccgactgt
     2461 gtcagctaga gaatcaccta ttacatttac tttgcataat cgaatgttcg aaagcgataa
     2521 gatgccaagt atcagtcaaa acgacggaat aaatatcccg gataactttc aaggagtaaa
     2581 agggttgaac ggcgttactt tcgctatata tgatgtctcg gatgaatttt acaagctgcg
     2641 gtcagaagga agtagtgtgg aagacgcaca aagaaaacta gcacaaaaaa gtgatagtga
     2701 aaaaatactg gcagaaaatg tgacaaaaac agtagacgag gaagagggca ttgcttcttt
     2761 tagtgcatcg gacaaagatg agcaaggtcg tgacgctgtt taccgatttg cggaaataaa
     2821 gacttcagat taagtaaaag aaaaatcagc accatttgtc gttgtgcttc ctgtgacaga
     2881 ttcatctaat cataaattga cgaccatcca cttgtatcca aagagcgaac aaaaaatacc
     2941 tgctgcactc acattaacaa aaacagtaga gaataagcag acagattttg cagatgggga
     3001 taaagttcct tatctcatca ccgtaacaat tcctgagaac atcaacgaga taggaaccta
     3061 tacgatcaaa gatacagctg atcctcagtt atggctagaa ccagaaacga tcgatttttt
     3121 gattggtgga gacaagattc atacatttca cacacaaaaa acaaaacatg gttttgtctt
     3181 atcagattct ggaaaagatc ttagttcgtt tgctgggaaa aaattaacga tccgttacca
     3241 aatgacattg aaagaaaaca gtgaaaaaac gacatttgat aacgatgtac gtttgacgac
     3301 tgacaatcaa acagtagaaa cacatcttgc catccaaaca ggaggcaaac aatttgtcaa
     3361 agtggatcgg caaacgaaac aaaaactagc agatgctcaa tttctagtca aaaaaaatga
     3421 ccaatactta gcgcaagaaa acgggataaa tcattgggta gccaaagatg attcaagagc
     3481 aacgattttt acttccagta aagatggaac attttctgtc catggattgt cttttggaag
     3541 ttacgaacta gtggagatta aaccgcctaa aggatatata ttgagtcaat caaccattct
     3601 ctttgaagtt gaaaaaggca gttatgatcc gcttcatcct ataccattgg aaatcatcaa
     3661 cgaaccaaaa gcaaatccga cgaataatgg aaaaacgcca gttcagatgc agacaaatgg
     3721 tccatcaaat gaaaaaaatg gaggaagctt tccaaaaaca agcgaagaga tgcttggagg
     3781 tttctcaatt ttagggttgt tgctagtcct ttcaacaggg acagcttggt tttataaaaa
     3841 acaacaaaaa ggaaacaata ggagggaaat aaaatgaaaa acatcaaaac aaaaatatgg
     3901 acgtttttag gaactgctat catgctgctt cctttcgttc tcggcctagg aactgcagag
     3961 gtttctaggc cgtatcacct acaccagaaa atgttacggt taatttgcat aaattgaaat
     4021 tcacttcagc accagaaaat caaataaaca acggaacgga attgacattt ccgaatagcg
     4081 aaccgttgaa cggcgttgaa ttcaacgtat atgacatcac tgctacgtac tatccaagta
     4141 aagacacagc agttccagca gacgctacac ccttcgcaag tgtaacgact tctggtgaag
     4201 gattagctaa cctgactctt ccaggtaaat ctgatgggaa agatgctgta tacgtctttg
     4261 tagaaacacc aaagcccggt gtcgaaacgt cgccaaatat cgtgttaagc ttaccctttt
     4321 ataacccgaa ttcatcagac cctgacaaac ctttagacac gattcaccta tatgctaaaa
     4381 atgtcatcaa agaaagcaat atcaaaatcc agaaagctgg gaacttttca ggggtagata
     4441 agttagcagg ggcgcaattc cagatcaaaa attcagagaa tcaattcgtg accggttttg
     4501 atgaaaacac tggtgtcgct ctctatgatc ctgctgagtc agcgttgaca ttcacaactg
     4561 atactgaagg atccatcagt gtcaatggat taatagacgg caattatact ttagttgaaa
     4621 caaaagcacc agatggttat caattaacca atccacaaaa agaaactact tttatagtaa
     4681 aaaacggaaa attgcaacaa acaaccggta tcatccatgg cgcagatgaa gccaatcttg
     4741 ggaatccaaa cggtgtagtc aatcgggaaa atacaactac ggataacacg attactgtag
     4801 aaaatgtcca aaactacggt gatttccact tcatcaaaca agatgtgaac acgcaagaaa
     4861 aattagccga tgcagaattt tctattctaa gagcaaaaga tccaactgtt aaattaatga
     4921 aaaaaatagc aaatcttgcc tcaggagaat acccatatgc ttggtcagat gaagtcacag
     4981 gttctgactg ggaagaagtg aaattgattt ctgattcaga agggaaattt tcaatcgaag
     5041 ggctgaaaca aggggcatac tgccttcaag aaacaaaagc accagaaggc tacgcattac
     5101 ctaagaatga agctgcgtac actgaattta cagtagatga tgatccaaca acttctgatg
     5161 aaacaacgat cgataaccaa cctaaaggaa tcttgccttc aactggtggt atggggatca
     5221 tcgtattcat cttggtaggt actgctttag ttggaggagc agtgatctac ttcaaaaaac
     5281 gccatcaaga aacagaagct taaactattg gaaacaaaaa tcatacgata aataaacgat
     5341 agtgattcaa atggggagaa aggttgtgac aagtggtcgg tctcttggta ttcgctcaac
     5401 aaacggcaaa ataggaggaa ctattttgcc gtttgttgag cgaatactca ggtcaaggcg
     5461 ctgatttcac aacctcttca tatacatatt agaaaatagg tggacatgat gaataaaagc
     5521 agaataagaa tactggacat cttcatggca atcattttag tcgttgggat cgggatcttt
     5581 tcttatcctt ttgtagaaga ttcactgaat gattttcttg cccagcaaat gatcatccat
     5641 tatcaaaagc aagcatcgaa taagaattca gaggaaataa aaaaacagca ggaaaaaatg
     5701 acaaagaaaa accaacagtt ggcagagcaa aacgtatcac cagggatagg cagtttcaac
     5761 aaagcagttg atgcaaaagc attaaaagat ttacctagta atgccttttt tatggaacat
     5821 atgcttggag tcatcgaaat cccaaaaatc aatgtcagct tacctatatt cgatcagaca
     5881 acggaaatat ttttgcaaaa aggaacgtca ttattggaag aaagttctta tccgactggc
     5941 gggaaaaaca cccatgccgt tctttctggg catcgtggac tcccagaagc aaaattattt
     6001 acagatttgc ctaagttgaa aaaaggagac cagtttttca tccagatcaa tggaaagaca
     6061 ttggcgtatc aagtagaaaa aatccaagtg gtcttacctg atgaagtaga ttctttgggt
     6121 atccaaaaag gcagagatct cgtgactttg cttacctgta ctccttatat ggtcaataca
     6181 catcgactgc ttgtgacggg gcatcgtatt ccttatcaag caaaagaggc aaaaaaagcg
     6241 atccaaggaa ttgatcaatg gaaaaaatgg aaatttttta tttggtttat cggtatacta
     6301 cttggatcaa tcggcttagt ttggctactc atcgcttatt tagattcttt ggccatcgct
     6361 aagcgaaact atcctttatc tttttatgta aagaacacaa acggacgacc aatcgaagga
     6421 atggtctttt ctgtaaaaac gctcaacggc aaacactata ttactagaga aaaagttcca
     6481 tttgtgaaag cttctgatga atacggacta gtgagatttt ctgatttaaa aggcaggaac
     6541 tatcgcttgc aacatgagga gctcctgctt aaaatccatg taaaacataa acattcgaaa
     6601 cagttttcaa tgaaattgaa aaaaggaaga tataaactgc gaaaagaaaa agaggtgtac
     6661 tatttgattg agaaagaatg aatgaagatg tccaaaaaag cagagggaac agactaaatg
     6721 aaaaaagaga gaagctgacg aaatggacgt gtaaactgct atttttggtt tactatctgg
     6781 aacgccagct tctatttttt tatgtttcag aagaatttta ttattatagt ataacttttg
     6841 aattagtttc cataacattt ggagcaaggg gttaaaccac gagcttgagc ttcgcttaaa
     6901 gtggcagggg tatacgtacc atttccacat ttatgtgtat ggtattttga accggtcggt
     6961 gtgacataga cagtttgttc attattttgc tcaggagctg cggcttgtgc ctgctgagag
     7021 gcctgttcag ctgctgcttg ctgtcgcgcc tgttctgctt gttgctgtgc gattgcttct
     7081 tgttgcgctt gttctgcttg ttgtcgtgca atttcttctt gctttttccg ttcttcttct
     7141 gcttcttttg ctttgattgt ggaatcaatt gcagcaagac ggcttgtcaa ctcttgtttg
     7201 ttttcaggta gcgcttggat agctgctgaa gcagttgcat agttttcttt tgtaggattt
     7261 gcttcagctt gtgacaaagc agattccgct tctgtcactt ttttttctag tgcagctttt
     7321 tccgcttttt gtttctcttc ttcttttttc ttttcaattt gttcttgttc tttcgatgaa
     7381 tctgccgtac tttcggagat cgtggtactg ctgctggaag cctgagctga acttgtgctt
     7441 tgagtagtag acgtagtagc accacaccca ctcaacaaac ttcctgcgac caaaaacaat
     7501 gcaactaatt ttttcactaa atttcctcct taagtaaaaa acaaccgttt tcaataaatg
     7561 tccttttcaa ttgtttactt acttgttata ataattgtgt tatgaatagt gattgaggaa
     7621 acttaatcta atcccgtttt gatacacggg attttttgct atagttctgt tattaagtat
     7681 gcaataataa aata
//
LOCUS       DACDHL010000110         7642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDHL010000110 DACDHL010000000
VERSION     DACDHL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.113"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            36..815
                     /locus_tag="IV228_002503"
     CDS             36..815
                     /locus_tag="IV228_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718353.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            856..1800
                     /locus_tag="IV228_002504"
     CDS             856..1800
                     /locus_tag="IV228_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8718354.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1816..2595
                     /locus_tag="IV228_002505"
     CDS             1816..2595
                     /locus_tag="IV228_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8718355.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2608..3306
                     /locus_tag="IV228_002506"
     CDS             2608..3306
                     /locus_tag="IV228_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8718356.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3334..4098
                     /locus_tag="IV228_002507"
     CDS             3334..4098
                     /locus_tag="IV228_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8718357.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            4111..5928
                     /locus_tag="IV228_002508"
     CDS             4111..5928
                     /locus_tag="IV228_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8718358.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            5942..7164
                     /locus_tag="IV228_002509"
                     /pseudo
     CDS             5942..7164
                     /locus_tag="IV228_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            7178..>7642
                     /locus_tag="IV228_002510"
     CDS             7178..>7642
                     /locus_tag="IV228_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8718359.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLML"
BASE COUNT         2672 a         1349 c         1601 g         2020 t
ORIGIN      
        1 ttatttatct atcatcaata tttaggagtg aatctatgga aaagccgttc gtcaagttat
       61 tggctactgt ggcggtaggc acaggcgcta ttgtgatttg cttgtttgga taccatctca
      121 ataatcagcg gcaacatcat caacggatca attacgcaga atctgccatc accaatcaaa
      181 aagacaccgt gacctcttta tcaaaagaag tagataagct gtacagtaca aaagaaaaaa
      241 ttttcctaaa tcctgaaata acagaagaaa cagtttcaaa tttgtcacat aaactatctt
      301 ctatcaaact ttcagcagat gattatgaca tcaaggaatc agaattacct aaagaagctg
      361 ctgcgatcca agaagaaaag aaagcagtct taacacagtt agaggatgca gaatcaaaac
      421 tgaagatcca aacggctgtc aataaattat ttactaaaaa cgtatcaaac tggcagcaag
      481 ctgtggatga tgtcatcatc aaagaaaagt tggcaagtgc agatgtcgcc catgtacgtg
      541 aaaatatgag cttttttaag gatagtgctt ggaaaacggt cgttatgcaa tatctaggat
      601 tcgctgatac ccaaattgca caagtcacgc aactagatca gctattcgat acgatgctga
      661 aagacggtca agtaacagct actgctactt acgatcaata tttgaccgca ctcagtctca
      721 tcgaacagat ccggaatgaa aaaataagag ccgcatacgc gtcaaaagca gaaacagtcg
      781 ctcagcaaat gggctatagc aatacgagct attaataagc gagaattcaa gcaaggctag
      841 caaacggagg aaactatggc aaaacgaaga agaagatcaa gagggaaaaa gatatttcta
      901 attattttag caattcttgt cgtattagta ttagcaatcg taggagtagg agcaaaatta
      961 tatctagatc tctcacattc tattgaaaaa acttacgaat cagtggaacg aacccaagga
     1021 gaacgaacaa aaccagttga tctggacaaa caagaatctt tctctgttct attgatgggg
     1081 attgatacgg gagatttagg tcgggtcgat caaggaagat cagatacgat gatggttgcc
     1141 acagtcagtc ctgaagacaa acagacaacg attgtcagca ttcctcgtga tacgtatgtc
     1201 gacatcgttg gacatgacac aaaagacaag atcaaccacg cttacgcatt cggcggagct
     1261 gccatggcga tggataccgt acaaaaatat gtagacatcc caatcgatca ctatgtttca
     1321 atcaatatga aaggtctaaa agaactcgtt gatgcagttg gtggtatcga agtcaataat
     1381 gacctcacct tctcacaaga tggctatgat tttacgatcg gtaaaatcac attgaacggg
     1441 gatcaggcat tgtcttattc tcgtatgcgt tatgaagatc caaatggtga ctacggcaga
     1501 caagaacgtc aaagaaagat cgtagaaggt gtagcgaaaa aagtgttgag tctgaacggt
     1561 gtaagcaact atcaatccgt tttgaatgct ctagagtcga atatgaaaac agacatgagc
     1621 tttgatgaaa tgaaaaagat tgctttagat tatcgggatg catttggtac gatcaagcaa
     1681 gatcagctgc aaggagaagg cttcatgcaa gacaatatct cataccaaaa agtcagcgat
     1741 caagaacttg atcgtgtgaa aaaagaatta aaagatcaaa tgaatctaga gaataaatag
     1801 gaaagtggag aaaaaatgga agagacaatc agcttgaaag aaatttttga aacattacgc
     1861 aaacacgtga taacaatcct catctcaatg tttgctggct tggccatctc aggcatcgtg
     1921 acgtttttca tcttgacgcc aaaatacagc tcacaagccc aattgatcgt gacactgccg
     1981 caatcagata ctgcaaatgt caatgatgtc aatacgaacc tgcaaatgat caatacgtat
     2041 aaggatatga tcgtcagtga tctagtacta aatgaagtca aagatcgttt agaaacagaa
     2101 gacaatgtaa aaatgactgc tggacaaatc aaagatgcca tctccgtcaa tcagtcacaa
     2161 aattcacaaa tgttctcgat ccaagcaaca agcacaaatg cagtgacggc tcaacaaatc
     2221 gctaatacaa cgtcacaagt attccaagag aacgccaaag acgtgatgaa tgtcgataaa
     2281 atctcgatca tctcaggtgc agttgccaat acgacacctg tttcgccaaa taataaattg
     2341 aatctagtga ttggcttagt gttaggaatg atggtaggcg taggtctagc tttcttattt
     2401 gaattactcg accggacagt aaaagatgaa aaatatgtga cggatacatt gggcttcccg
     2461 atcttaggga ctgtaccaga aatgtcagcg aaagaattga atgctggtat ccgtaaaaaa
     2521 ggaccagctc cgacacaacc aaaagctaca aacgataaaa cagcgccatc acgcagaagc
     2581 cgctcaagag tctaggagga aagaaaaatg gcacgaacac agaaacaaaa agcagcccaa
     2641 actcatgcag tcagtttggt cacactcaca aacccctctt caccaatcgc tgaacaatat
     2701 cgtacgattc ggacgaatat ccagtttgcc tccgcagctg gtcagcaaat taaaacgatt
     2761 gttgtgactt catcaggacc tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt
     2821 gtttttgcca aatctggtca acgggtgctg ctagtcgatg cagacttacg taagcccgtc
     2881 atctataaaa cgttcaagtt gaacaacgca agcggattaa gtaccgttct tagttcatca
     2941 ggaagtgtag cagatgagat ccaacgaaca ccagtcgaga atttgtcgat tctgccaagt
     3001 ggacctaaac caccaaatcc atcagaacta ctaagttcgc caagaatgga tcagatttta
     3061 gcagaagcaa gacaactatt tgatgtagtg atcttcgaca tgccgccagt agttgccgta
     3121 acagatgcac agatcatgtc ttccaaaacg gatggcacct tattggtcgt acgtgaaaat
     3181 atgtcacgga aagaatcatt gactaaagca aaagaattgc tggatatggt acaagcgcga
     3241 gtccttggtg ttgtatacaa tggggcagaa cacagcaaag atgctggcta ttactacggg
     3301 aattaagaaa taaaaagaga ggagacaaga gaaatgatcg atctacattg ccatatcctg
     3361 ccaggaatcg atgacggagc agaaaacata gatgccagta tcgccatggc tgaaaaagcg
     3421 attcaacaag ggatcacaca tattttatgc acaccccatc ataataatgg aaaatacagc
     3481 aatgaaaaat cacaagtcat ttctctagtc gcttcgttac aagcagaatt agagaaaaga
     3541 caattgcctt tgacgttatt ggaaggacaa gaagtacgta ttaccggaac actgatcgaa
     3601 gacatcgacc gagatgaaat tttatttaca gatttagatg atacatatct tctaatcgaa
     3661 tttccgacac tggaagtgcc gttatatgca gaacagctct tttttgagtt gttacaacta
     3721 ggaaagacac cagtgatcgt ccatccagaa cgcaacgcgc attttcgtaa agacccgaat
     3781 catatgctgc cattcctaga tatgggatgc ttggcccaac tcaccgcacc aagttatgtc
     3841 ggtgtatttg gaaaagacat ccagaaaaca gccaaaatga tggtcaaaca taatcttgtg
     3901 cagatggctg cttctgatgc ccatggggtc tctaagcgca ccttttactt aaaagagtgc
     3961 tataagcaga ttgccaagga ctttggtaag gaaaaagtag tacagatgaa acaagtcgca
     4021 aaggatctga tcaacgggga tgaaatcagt tatccagtct atgaagagat aaaaaagaag
     4081 aagtttggtt tattttaggg ggagtgtaag gtgtttgtat taacaagaag aagaaaaatt
     4141 gccacattgg ttgttgtgga cagtttgtta ttgatcgctg caaatattgc agcagctaaa
     4201 ttcatgaagc cgtttgtagc tataccaatg gaccttattt taatctctac cggtttgtcg
     4261 attggttttt atttatttta tggtagttta ttcaaagttt tcactcggat caatcgctat
     4321 acaaatttaa gagagatcat tgcaattttt ggttcactat ctgcaagtga cgtctcaagt
     4381 atcctcatac tgctgttcat taatcggcaa tacagtttac ggttagttat ctttgcttat
     4441 ctgctttcgc tcttattgat catcggtagc cgattgatct ggcgtatcta catcgaaaca
     4501 aaaaatatga gatatgtatc agcggattct gctaaaaata cattgatcgt tggtgctggc
     4561 gaaggcgggc gtattttata caacagcttt ctgggatcca aaacggctca agatatccaa
     4621 gttgtcggat ttgtagatga tgatccgaat aaaagaaata cgtatctttc aggaaaaaaa
     4681 gtattaggtg cattaaaaga tattccggaa ttgattgaaa aatatgacat tcaaatggta
     4741 acgatcgcta tcccttcctt atcaagaaaa aaattacgaa gaatcttcga gttagtcgaa
     4801 tccgctcacg tgaaagtcaa cacgatgcct tctatagaag aacttgcttc aggaaaaatt
     4861 agcgtatcaa aattaaaaac aattgacgta gtggatttac tgggaagaga tgaggtaaag
     4921 ctggatattg aatccatcaa agatcagatc acagacaaag tcattcttgt caccggcgca
     4981 ggaggatcga tcggttcaga aatctgtcgg caaatcattc aattcaatcc tgcaaagcta
     5041 ttgcttttag gacatgggga gaactcgatc tacttgattg accgtgagtt acgcacgcat
     5101 catcaaaatt gtcctacaga aatcgtaccg atcatcgcag atatccaaga tcgcgagaaa
     5161 atcaatgaga tcatggaaca atatcatcca gatatcgtct accatgcagc tgctcataaa
     5221 catgtaccac tgatggaata taatccaaaa gaagcagtca aaaacaatat tttcggtaca
     5281 aaaaatgtag cagaagcagc taaagcagca aaagtgaaaa acttcgtcat ggtatcgaca
     5341 gataaagcta acaatcctcc aaatgtcatg ggttcaacaa aacgaatcgc tgaaatgatc
     5401 gtgactggat tgaatgaaga aggctgtacg aaattctcag ctgtcagatt tgggaatgtc
     5461 cttggttctc gcggtagtgt gattccagta ttcagagaac aaattgctaa aggtggtcca
     5521 atcactgtaa cagactttcg tatgacccga tacttcatga cgattcctga agctagtcgt
     5581 ttagttattc aatctggcgc attagcaaaa ggtggcgaaa tttttattct agatatgagt
     5641 gaaccagtga agattgtaga cctagccaaa aacatgatcc gattgagcgg gtatagcgaa
     5701 gacgaaatcg aaatcattga aacaggtatc cgtccagggg aaaaactata tgaagaacta
     5761 ctgttggata aggaaagaaa tgatgaagca gtttatgaga aaatctttgt aggaaacatc
     5821 aaaggttact caatacaaaa agtgatggat tttgtcaaga gcctgccgca agacgatgaa
     5881 caattggcta aagatatcgt cacttttgca aatgcttcga ataaataggg gggaaatata
     5941 aatgaataaa ttagaaaaac gtcagatacc attttcaccg ccagatatta ctgatttaga
     6001 aataaaaaac gttgtagaag tattaaaatc tggatggata actacgggac caaagacaaa
     6061 aaaattagaa aaaaaattac aaagtggtta ggaacttcta aagtagtttg tttaaattca
     6121 caaactgctg ctgcagaaac agggttacgt ttgctgggaa taggagaaaa tgatgaggtt
     6181 atcgtcccag cttatactta ttctgccagt gcatcggttg tatgccacat aggcgcgaac
     6241 ctagtattaa tagactgtca acaagattct ttggagatgg attacgatgc attagaagca
     6301 gcaattacgg agaagacaaa agtgattatt cctgttgatc ttggtggtat accttgtgac
     6361 tacgaccgta tttttgagat tgtggagaaa aaaagaaatt tatttcaacc tagtaatgag
     6421 attcagaaag cttatggtcg tataattgtt atggcagatt ctgctcatgc atttggagct
     6481 aattggcatg atcagatggt tggtaatgtt gctgacttga caacattttc atttcatgct
     6541 gttaagaatt ttacaactgc agaaggtgga gctttagtat ggaaagagca cgacaaacta
     6601 gataatgagg caatgtatca tcagtgccag ctgctttctt tgcatggaca gtcaaaagat
     6661 gcacttacta aaacacagtt gggtgcatgg gaatatgata ttgtgggtcc atggtataaa
     6721 tgtaatatga ctgatgttac tgcatcaatt gggttggcac aatttgaacg ctatcctaat
     6781 attctgaaaa gaagaaagaa tataattaag aggtatgatg aagcctttca acaactagga
     6841 attgaagttc ttatgcatta tacggatgat tatagttctt caggccattt atatattact
     6901 agagtaccta acattagttc tgagcaaaga aatgaaataa ttataaaaat ggcagaacaa
     6961 gggattgctt gcaacgttca ctataaacca cttccaatgt ttacagcata taaaaatatg
     7021 gggtttgata ttaataacta tcctaatgcc tatgctcatt ttgtaaatga gattacatta
     7081 ccattgcaca cgaaacttac agatgaagaa gtagaatacg taattaataa ctatagtaag
     7141 attgtaaagg atttggttgg atgataggag aggcaaaatg agaaagtggg aagatttgcc
     7201 aaaagaaatg cagaatgatg aaatacggta ttattatgat atcctcagta aaaaaaaatt
     7261 cagcttagtg gtaaaaagag tatttgatat tttagtgtct gcaactttgt tactgacact
     7321 ttcatggtta ttttttattc ttgcaatagt gataaaaatt gattctcctg gacctgtatt
     7381 ttatagacaa gaacgtatta ctcgttatgg aaaaaaattt aaaatttata agtttcgtac
     7441 tatggtaata aatgcagata aagatggcac tcttgttact gtgaaaaatg atgatcgtat
     7501 tacaagagta ggaaaggtaa tccgtaaata tagattagat gaaattagtc agctaattaa
     7561 cgttttggtc ggagatatgt cttttgtagg aaccagacca gaagttggaa aatatgtaaa
     7621 gtcgtattca ccattaatgt ta
//
LOCUS       DACDHL010000111         7625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDHL010000111 DACDHL010000000
VERSION     DACDHL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.114"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IV228_002511"
     CDS             complement(14..706)
                     /locus_tag="IV228_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8718360.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IV228_002512"
                     /pseudo
     CDS             791..967
                     /locus_tag="IV228_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IV228_002513"
     CDS             complement(1143..1712)
                     /locus_tag="IV228_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8718361.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IV228_002514"
     CDS             complement(1786..2109)
                     /locus_tag="IV228_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718362.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IV228_002515"
     CDS             complement(2263..3075)
                     /locus_tag="IV228_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718363.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IV228_002516"
     CDS             3429..4277
                     /locus_tag="IV228_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8718364.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IV228_002517"
     CDS             4422..5363
                     /locus_tag="IV228_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8718365.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IV228_002518"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IV228_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacct
//
LOCUS       DACDHL010000112         7419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDHL010000112 DACDHL010000000
VERSION     DACDHL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.115"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..533
                     /locus_tag="IV228_002519"
     CDS             <1..533
                     /locus_tag="IV228_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8718366.1"
                     /translation="TSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            609..827
                     /locus_tag="IV228_002520"
     CDS             609..827
                     /locus_tag="IV228_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718367.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            814..3492
                     /locus_tag="IV228_002521"
     CDS             814..3492
                     /locus_tag="IV228_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718368.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3483..3680
                     /locus_tag="IV228_002522"
     CDS             3483..3680
                     /locus_tag="IV228_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718369.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3684..4295
                     /gene="mobC"
                     /locus_tag="IV228_002523"
     CDS             3684..4295
                     /gene="mobC"
                     /locus_tag="IV228_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP8718370.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4280..5671
                     /locus_tag="IV228_002524"
     CDS             4280..5671
                     /locus_tag="IV228_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8718371.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5887..6201
                     /locus_tag="IV228_002525"
     CDS             5887..6201
                     /locus_tag="IV228_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718372.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6194..6688
                     /locus_tag="IV228_002526"
     CDS             6194..6688
                     /locus_tag="IV228_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718373.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6756..7070
                     /locus_tag="IV228_002527"
     CDS             6756..7070
                     /locus_tag="IV228_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718374.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7169..7414
                     /locus_tag="IV228_002528"
     CDS             7169..7414
                     /locus_tag="IV228_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8718375.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMDRLQLTRQKN"
BASE COUNT         2851 a         1002 c         1434 g         2132 t
ORIGIN      
        1 acacatcaat gaaaacaaga gacaaaaaca attttgctga tagttcaaaa aatgaactag
       61 aattatataa gtcttataaa gatagtaaag atattaaaga atcacataat gatgatcttc
      121 ttgaatcact ttcaactaaa aataataaat tagatgaaga attgatcgaa acatttattg
      181 aagaaaatct tcttaaacaa atatggggag aaagtattgt gaattgctta aaactggcta
      241 gcaatagtga ttatcgtcaa ttcgacaact ggtacaaaaa attcaaatat gcgattagat
      301 ctgctgaaaa agaacaaaaa attcaactga aaattatcta tgaaatttgt aataacaagt
      361 attttgtaga tcatgttaga gaacagctat caatgacttt acgtgacttg atacgaagag
      421 caaaaacaga tcatcgtatc aaacaaaaag acgattatat ctttgcttct ctaaaaaata
      481 aagcgttaga actaattgaa ttgcaaatca gcgaaggaat agataagcaa taagaataga
      541 attgagtcaa atgaaatgga ggggtatctc cacttcattt gagaactttg aatataggag
      601 gggttatttt gattaaaaat cgtttaatag atcaagtttg gtgtctagat caaaatacag
      661 atagtctcga attaatcgag actatttgct tcaacactat agttctcgat ttaagagctg
      721 aaaatgataa ttgtgtaact catgtcataa ttaatcaaaa aatggctcag aagctctctg
      781 agagcctcag aaaatgggct aagggaggta acgatgaaaa taattgaaaa aaagattgcc
      841 aataaaaaaa taaattggtt ggttgttaat tttcttgttc tgatagctat gattattttt
      901 acaagtaata ttgctcatgc gagtattttc aattggtttg atgacacaaa aagtcaaaca
      961 gagtttttga ataattctct ttatagacct tacctaaaaa agtacaatgg agatttcctt
     1021 agtcagttcg ggatttggct cggatgggct gtggtaaaag gcatgtttac tgtgacagat
     1081 tcgattcaaa atatgattcc agatgtgctg gatttgttca atttcattga aagtacaggg
     1141 ctaaataatg tttatcaatc agccgtaaat acaattgtca ttggattaat gatcctttca
     1201 ttgatgtttg ttggttacaa aatgattact ggaaaaggaa cagtggattt aaaatcggtt
     1261 ggaatgaacg tagtgatgtc tatagcatta atacttttaa tgccaaccat gatttctagt
     1321 ggtattcaat ttgctaaaac attttataag gactctacta cgattacaaa ttcagatgac
     1381 ggagtcgctt ggtcattgat aaaacaagga gtaacagatt tagcatatat taataaaaca
     1441 gatcagtact caagcattga taaaacggag gatcgaaata atttaacacg aaaaaatttt
     1501 caacagacgg atctcacaca agtgttaacg gataaagtga ttgataaatt ggaaaaagaa
     1561 aatccaattg cggacaatct tcgttatgaa ttagttgaaa attctaataa tgaatttgtt
     1621 gctacaaagt tttctgacaa cttcttatca acattttctg atagcttgaa atcaggctat
     1681 taccgatacc aagcaaatct atgggggatt tcaattgggt tgacagcgtt agcaatcgcc
     1741 tatgtgttca gcgcgtttgt gatcattaca gcaatactag aacttgcatt taaacgtgtg
     1801 ttaggtgttc ttgtttttgc aacagatatt gagacaggac agcgttcaaa agttgttcta
     1861 tcagatattc tacaatgtta cttaactgtt gggtttcaag gatttgggtt atcaatgttt
     1921 gctatgttta tcaattttct gaactcagga caaggtatct caacaaatat ttttataaaa
     1981 acaattgctt atatctgtgc cgtttttgtt ttaatcaaag gcagtggaac agtcatgaga
     2041 tactttggtg ttgatattgg actcaaagaa ggctatgggc aacttgcttc tgcttttgga
     2101 atgggcgcaa tgctgtttag aaaaggatca aatggattta atcgtgctaa aggaagcgga
     2161 gaaggagaag atcgaaaacc agaaaagaat ttcggcgaaa ctctatcaaa aaaagctggc
     2221 aaaactggaa gagctttggg atatgcccat gaacgtggat tatccggttt agcttcagat
     2281 ggagctacaa tggctagtga aagagcaaca aaaccattta agagtatgcg tgatatagct
     2341 aatgatacca agaataagtt taaagaaggt ttagatgatg gtacagtgtc cgctattaat
     2401 aaaaatagca aacctatgtt agctaaaaat aaagaagatg aaactggaaa atatgctgat
     2461 tccgtgccaa cacgtctttc agatagaaaa gatggtgcta aagttgaaaa tgccgataga
     2521 attatgtcta gttctgaacg tatgcgtgaa gcaatgaaaa ataatgctga atcgaataat
     2581 aatcctgcaa gcgcaattca gcaaaaagtc caacaagata ttgaagaacg taaaaatgct
     2641 atgcacggtc aagctaagtc tgctgaagaa ttaattaatc agaagcgtca tgaagcgaaa
     2701 tacacgcctg aagcaatgaa tcgcgaggaa ataatgttaa ggaaacgtgt agaagggcgg
     2761 acaggcgcta atattgacgg aaaagaagcg ttgaccaaag aaagaattca agaagctaaa
     2821 aattcaaata caggattaga aaaattagtc aaagaaaatc cacaaaattc tacaagtagt
     2881 catggtggcc gatcagtaga tgtccgagaa aatattcaag gttcactgaa tggtcatgtt
     2941 ggacggaatg ttgatgtaag agagaattta caaagttctt caaatgctca aggtactaag
     3001 acaattgatg ttcgagaaaa tctgcaaagt agcagtacaa gtcaaccaaa aacagtagat
     3061 gttcgagaaa atgtacaaaa ttctacaagt agtcaagggt ttaaatcagt ggatgtccga
     3121 gagaatgtgc aaggtagcag tgcaagtcaa ccaaaaacag tagatgtccg agaaaatgta
     3181 caaagttcta caagtagtca aggattcaaa acagtagatg ttcgggagaa cattcaaaaa
     3241 gatagtggag aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca
     3301 gcgaaaaaat ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat
     3361 gataagggat caaaatcaag acgaagattc acttatgaag acaatgagtt attcagagat
     3421 acacttaatg atcctaatcc acttttcgat aatttattaa gaaataaaag ggggggattg
     3481 tcatgcaatt aattctaaag attagtacgc atgatttctt agaacttgac gaattatgtg
     3541 aatcagtaaa gaacgcaaca aatgaaaaaa tcacggtcac taattatatt gaactgatga
     3601 ttcaagaaga attaaagatg aaaaatgaaa tagagagcaa tttcaaatgt tgctctctag
     3661 aaaaaaatgt gaggggataa tcaatggatg ataaacaaaa aatgtacgaa aaaaatcgca
     3721 aacatagtga ggccttaaat tttcgtgtcg ataaaaagac ttatcttgaa ctgacattga
     3781 ttgcaaaaca aaggggaatt tctagagcag acgtttgccg tgagcggtta aagggactca
     3841 aatatgaacc gccgttgtta gctgaagatg atagggtagt tttaaccaaa cagttaaatc
     3901 aaattggtaa caatttgaat caagcggtaa aggcattaaa tatcattgct aaatatttta
     3961 gagaagaatc tgaagataaa aaagaaaaga gcctacattt tagttttgat gctagccgtt
     4021 acaacatttt taaagatgaa cttgcgaata gcatagcaaa aaagttgatg attagttatc
     4081 cagatgacat ttttttggag tccaatcgga gtgccccctc tttttttaaa ttatcagaag
     4141 tagaaaaaga atatttcgaa tattggatag aaaagcaaac tagaccgggt acgaaagaag
     4201 agtattccca aaaagttgat cggaatttaa ttcactcaat tcagattatt gcaagtttaa
     4261 aagatgaggt gaaacaactt tgggagctac tataacatcg aaaggacgca agaaaaattt
     4321 atcctcgcta ttacaatatg cagctaatcc ttcaaaaaaa gaaaaatgtg ttgcagtctc
     4381 aggaataaat tgtagtgatg atagtgagga agcactgcaa gaaatgaatt tagtacgtac
     4441 caagatgaat aaatctaaag atatagcaca agcgtttcat gttattcatt cgtttgataa
     4501 gagtacttct aaagagttaa gtattgataa aatgcacgaa atagctgtcg aatttgcaga
     4561 gaaagctttt ccaaattcgc aaatcattgt tgcaagtcac aatgataaag atcattttca
     4621 tagtcatctt gtcattaata atattaacat ggaaacatgt aaaaggttgc gtattgaccc
     4681 taaagattta gaactaatgc gaaaaattaa cgatcaagtc ctcaatgaac atggtcttga
     4741 atcagtgaat aatgagtact atgaaaactt agaaaagaga accaatcttt attcaaacga
     4801 tccttctaag atgaaatatg gaaaaattaa ttatcaagag tttgtgcaaa atgcgatcga
     4861 agcaacgttg gaaaaatgcg aagtgaattc ttttgaagat ttttcggatc atttggcaaa
     4921 agaatataac attgaagttt atcaattttc aaaaacaaat aacaaacttg gctatgttct
     4981 ttacaaacaa ggtgtaaatt tctatgagcg tagaggtgag ttaattactt taggaagaga
     5041 taaacaatct agaaaagaaa aaagtcagta tgtcgaacgt acttttagtg caagaaagtt
     5101 agggaaaaga tattcatttg aatctgtttc tgaacaactt gaaaagaatt tagaagaatc
     5161 acatataaaa aattttttta atatggaaag agagcaagtt atgtcgctaa ttagtggagt
     5221 aaattacaaa actggtgagt taaatgtacc gtttgtggaa ttagattcca tcgcacaaaa
     5281 gcaatttagc gaccgtgaag gcgatgaaaa attcattaaa tatgatgact tcttgcgaga
     5341 ggtagacaag cattttgtaa atacaatgtc tttagaagat gatcaagaat acacgattaa
     5401 acttcatacg gatgtgaatc ctcaaacttt acgtagtgcg aaaagtaaaa caatttctat
     5461 taaaaagact gatcagaaag agttatttct accggcaaag ggttttgaaa attgttctca
     5521 tacaccttct gctaaacaga ttcactatca aatcagtcgc ataatgaatg acgatgtaaa
     5581 aatgtatcgt gctaaaaagg attatgaatt cacacaacaa atgattgaaa atcagcaaaa
     5641 aaggcaacaa aattttgaaa tggaattctg aatgggcttg ttagtggact ttccagtttt
     5701 ttgcctatga agagttgctt ggaaagaagc agtttttagg acatacgcca gctacccatc
     5761 tacttgccag tcaatccgcc ccgctcacgc taggctactt tctggcaagt cagatggcgg
     5821 gtaaatctct gagggagcaa gctccctaca gactccctcc caaaaaaaga aaggtggaag
     5881 aaaaaaatga actatccata ttttaaagtc tcggcttcag aagaaactaa agaaatattc
     5941 aacaattttt acaaccaaaa caaaggaatt tttggttcaa aggcaaatat gtttcgtgtt
     6001 atggtttcaa atttaccagt gttagctagt ccatcaaata ataaatttaa tgatcctgaa
     6061 agcattaaat ttgaacaaaa aatttcagaa ctagaatcaa tgataagtaa tgaagtaatt
     6121 gaaaagctgg atgacattga tcaaaagtta tcgtattctc taaaaaacaa atacaaaacg
     6181 gaggagaaaa aagatgttta aagtttactt cccgtttcag tggctcgtta aaggaattct
     6241 tttagtgcta ctaggattga ttctttttgg aagttatcta acaattaaag ccagacattt
     6301 tccagaagaa aagccaataa aaattgtaac aaaagattta aaaataggtg aaacttatca
     6361 ggcagatggg gttgaactaa catttaaaaa tattgaagtg accgaaaaaa gaaatgaatt
     6421 tgcacaacat gatcgtgtat tacaagtgac ctatacactt ataaatgata cagaaaatcc
     6481 gatacgtcca caacaatggt cgattgaaaa taaggatggt gaagaacttt atgagtatga
     6541 tatgatattg gataatccag agtatattga aagtggcaaa gaagaaacag taaattacta
     6601 ttatgaatta ccgattgaaa ggcaggacta ccaaatcgta gtacgtagag agaatggtta
     6661 catagttggg ttgccattta ctttataagt tttattttgt tagtagtttt taaggtaata
     6721 tcagttttgt gatttttgaa aatggaactg ggagaatggc tgaggaaaat aaagttgttc
     6781 cgtttgaaac attagtgata aataaggaag agtttgagca actttttctt gattttttta
     6841 ttcaatatga catgatttat gattttagtt cgtcactaga tttatttgaa ttagaagcag
     6901 aaataataga aaacagtcga gagaagaatg gtaaaatcat tatttcagta gaaaaattca
     6961 atgaattatt ctcacaaaca tttgaaatag atccagacta ttatcaacca caagaagttt
     7021 ttcgagagtt tagaaataag attattggtt ttagtcaaac tacaaaatga aagcttaata
     7081 aagcaagtgt tgatagaatt attcctcaac gcttgcttta ttttatgggc acttcataat
     7141 agaggtatag agaagagagg tattcatttt ggatactatt actccaacaa acttcaaaga
     7201 aaatataggt aaagtgttgc aagaagtagt tcattcaaat tctgaaatag aaattactag
     7261 ggattctaaa aatggattga acgatggcgt cattatgatt tctaagagaa aatgggagtt
     7321 gttacaagaa gaactttatt tacaacgaac gggtacatgg gcttatgtat ctgatttaat
     7381 ggataggcta caattaacta gacagaaaaa ttaaggtgt
//
LOCUS       DACDHL010000113         7307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDHL010000113 DACDHL010000000
VERSION     DACDHL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.116"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..892)
                     /locus_tag="IV228_002529"
     CDS             complement(<1..892)
                     /locus_tag="IV228_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8718376.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
     gene            complement(1269..1907)
                     /locus_tag="IV228_002530"
     CDS             complement(1269..1907)
                     /locus_tag="IV228_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718377.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1911..2681)
                     /locus_tag="IV228_002531"
     CDS             complement(1911..2681)
                     /locus_tag="IV228_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8718378.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3099..3515)
                     /locus_tag="IV228_002532"
     CDS             complement(3099..3515)
                     /locus_tag="IV228_002532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718379.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3566..3947)
                     /locus_tag="IV228_002533"
                     /pseudo
     CDS             complement(3566..3947)
                     /locus_tag="IV228_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4073..4738
                     /locus_tag="IV228_002534"
     CDS             4073..4738
                     /locus_tag="IV228_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718380.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5135..7168)
                     /gene="brxL"
                     /locus_tag="IV228_002535"
     CDS             complement(5135..7168)
                     /gene="brxL"
                     /locus_tag="IV228_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HAP8718381.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         2347 a         1422 c         1083 g         2455 t
ORIGIN      
        1 tagaatctga agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg atttttacat
       61 tagcgtcttt caatacttcg ccaattactt tattctggaa ggtactgtca cttaattttg
      121 tgtctgttgc aatttctttc aattcttttt tgtatttatc catgtcgttg cctttgtttt
      181 ggtttttcac cattttaacc acataatatt ccgtagtata agttgaagca ttggtagaag
      241 tgatcacatc agaaacttga ccatctttca acttaaaggc tgcttctttg acttctgccg
      301 gaaccgttgt agtagtggaa tcaaatttca cttttccgcc atcttctttt gtcgtagtat
      361 cagttgactt gtctttggct aatttagaga aatcgtctcc cttatcagca gctttcttca
      421 catcttttgc atcatcttca cttgctactt ggataatttg agcttccact tctggatgga
      481 aagattccca agccgttttt aaatcttcat cggtcaattt gatatgtttc tttagacctt
      541 cttgaaaggc tagactttgt ttgatactgt ctttgaatgt ttgttctgtg taacctgcag
      601 atttcaattg tgaatcaaat gaatcaccca attgtttctt tgtttgatca tattgggaat
      661 cgatttcttt ggttgatacg tcatctccat atttatcttc aaatacttga taaacgatca
      721 actgttgcac agcggaatct gctccttgca tttttttcaa gctatccatt acttcttgtt
      781 cgctgattgt accgccttta taactaacgg ctgttttttc accggcattc gtacagcctg
      841 acaataagcc agtgcctagt acgatggcaa gtgctaaaaa tctttttttc atttatcaat
      901 tctcctactt ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga tgaaaatcat
      961 caagaacacc tttactaatc tgctcgatac gtcctgaata gatataaggc tacacaatcc
     1021 agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc acaccttaat atactacttc
     1081 ttttgttacc gtttttcaag tctatcttgc gttccgtatt taacgtaact aattcttaga
     1141 acaaatgtta tcgattttgt ttttcttctt ccgctgttgg gatattcaaa tcagacactt
     1201 ttatgttcga ttttccagac atttttttgt tgtttttatt agaaaaagtt tgttttttca
     1261 tttgttttct aaagcatgat cttcttgttt cctattttgg tcagatatgg atcatgcgtg
     1321 accacgataa ctaacgtacc ttgggaagcc tgttgcgata agatttcaac gacttgctcc
     1381 ttatttcgtt tatctaatga tccagttggt tcatcggcta aaatgatttt gctattcttc
     1441 aataagactc tcgcacaggc gattctttgc ttctcaccac cagataatgt gtagataggc
     1501 gtacttagct tcttgtttat atttactttt ttcaatgcct catactttgc tgttcgctta
     1561 tctgaaacat ttaaattcga aaacttcaat ccaatatcta aattaaaatc gattgtttca
     1621 tattcaatca acccaaaatc ttgaaaaaga aaagagaggg catttctttt tatcaagcgc
     1681 ttctctcttc cagttaaatg ttttgtttct tgattaaata aataaaattg ccctgaactg
     1741 aattgatcat tcaatcctaa tatattcaat aaagtacttt tccctgctcc gctctctcca
     1801 actacagtca caaaatcccc agcatccgca gtgaaacgat ccaaatgaag aatcgttttg
     1861 tttcgaatga tttttttcgc accttttatc actatcaacg gctccaattg ttaacctcca
     1921 ttcatcagca ataactgact tttcttttct gtagacaaaa taaaaacaac tgaaagaata
     1981 aaatcaatga agctaaagaa aaaaatatta aaaagatatt taggctctct ttctataaga
     2041 aaaaggaaga ttacacttgt cgttccccat ttgatgaggt tcatcatgaa gaaatcttta
     2101 tacgtttcaa agagcgaata accacttgaa cgaaagatag cgattttccg acaattgtag
     2161 acaaaatata tgcataacat gacatagctc aacaagaaga aagaaaagaa agtgagtaaa
     2221 aatatctgta tcaagtaatg aaatgatctg cttaacactt tgacagctat ctcttcagtc
     2281 tgagtatacg gaaatgataa ctgcaagtta tcacgtaact gactttcttc aatcagctca
     2341 aataaccttt ctttcgacaa agagcctatc ttgatcttta acggtggata gatttctccg
     2401 ctgagaatat ttctttccca atacgtactg tttttcaatg tcagtatttc aacaaccgga
     2461 taatgttcga tccaggggat agaaggaata aatgtataaa tgggttgatt gttttttata
     2521 aatagaaatt tcggttcttt ttcactgatt tcttgataat attctgttat ttgcgccagg
     2581 tcatttttta aatctgtatg tctatatcgt tcagggatca atataacagg gtgcgtttct
     2641 ttttcggtta tttggatcaa ccttccagag acatcaatca aaggaaattt cttaagataa
     2701 ttcagattta tttttatccc tctgccaaac tgttcatttt cttcattcaa gtaatctgat
     2761 acattgacta atatacttcc gtttttattc aaagtttcat ataattcctg atcggctgct
     2821 gatgcaaaac gattggaata aataaaatcc acgtggtttt tcccgatggt tactggataa
     2881 aatacatgat agtcctcacc agcgatttct tttggtgttt catgggaaac agctaactgt
     2941 gaagaagaaa aaacgatagt tgctaattcg acattggcaa cggtcaaaag aagagaaaac
     3001 aacaatccaa atgtaaaaag aatgtattgg atatagttga acgcttctct taacgaagcg
     3061 attttttcaa ctaataaaag agaaatcaaa gagataccct atattccgct gaacaagaaa
     3121 acgtacatct ggaacaaata aagaaattgc tccttataat acataaaaat aaaaccacca
     3181 atgattcctg caattatgaa tgtaataatg attttctttt tccccaataa acaccacaat
     3241 acttgatgta atgaaaagcc attattcaac aataaactga tttctttatt catcaataag
     3301 aaaaaggatg ataacaaaag gaataaaaga atcaatccaa tagttaaata cactcgataa
     3361 ttatatacgt ctgtacttga aataaccgaa tacgaatagc tattttcaaa gtcttcaaaa
     3421 atattagtgg cataatcagt gtgaaatgct tgattgaatc gttccacaaa tagtaaaatg
     3481 aaccgttcat aggcttcttt ttctctcgtc atcaagaaaa attcaccttc aaaatctttt
     3541 attttgatcg attcttcaat ggacgttacg cgaaaggagg tattagaata gggtaccttc
     3601 tcgtgagaag atttgacatc cccttgataa tacaatgtca tttcaagctt tggctgtatc
     3661 ttgaagttcc aagatgtatc tacttcattg gattcattta ctgttttttt catataagaa
     3721 agctcagttt ctgatgagac attttcaaat accatgatta gctgctcata ttcttcttgc
     3781 ccaataaaat gttgtggcaa tcgaatcgga gtataagtat ctgtttgatg gaaaaattca
     3841 tttgtccgat agagtacctg tttatttata ataaccaagg caaaaccact catgataccg
     3901 gtcactatga ttgtgagtaa aactgtgaac ttttttttga ttatcaccac tctcccttac
     3961 ttaaatgatt ctttgataaa atcataccgt ttttcatcaa aaaaacgtat ctatcaaatg
     4021 tataggtcat gtagaataaa tctatatact gtagaatcga aaggagaaga agatgaacat
     4081 cggagaaaaa atacgaactt accgcagaaa ttgtaagctt acgcaaaaag aattaggcga
     4141 aaaaatcggt gtttccgata aaacaatctc tagttgggaa aattctcgta caatgccaga
     4201 ccttgaaatg ctgtcacttt tacatcagac tttaggtctg ccgattgatt ttccttctat
     4261 tgaatcagct accgaatcaa tagaaccaac attgccggat gaacctcttg ttatcaagaa
     4321 atcttattca tcaaaacaac ccttctcact caaaaagaca ctatctttct ttctgctcgt
     4381 cttttgtttg gtctatcttt ttcttatcaa ttatcaatat aaaaatgaaa cggttgatcg
     4441 attcaatcct tttttgaaac cacaaatcgg ctataccaca ctccctgtag gatacgtgga
     4501 aaattccatc gaacattggg tatgtaatga tatctttgtt catggtacat ggctgtcttt
     4561 tcacgctgga gaggtagaaa aaggtaagaa tcatgctgta atcaaacaca aaggaaaaca
     4621 cgtcatgaac gttcgcctgc tcacttggga agccattccc tcagattacc aagaaatcct
     4681 catccaagac tatgacgatg ccttaaaaag aagccctcag gagccagata ggcattaagt
     4741 aggagatgaa cgtagagata gaaaaaatca atgcacatga tcttatcaaa aaaaaacagg
     4801 ggtacaaatt aattgaaaaa gctttgagat gaaagttcct aaataaaaaa gttcagaatg
     4861 ataggtaaaa aaatgactaa ctattctagt attaggctat ttgtattatt cttctgttat
     4921 ttttatatca aaaaatccag aaataactaa atataggtat ttctggctta tagtataata
     4981 tttacgccac agcccccaat gattcttttt ggaatcatga tcaaggacgg acatacacaa
     5041 aaatattttg cccattcgat ccgaatagat taacgactaa tctagtaagt aatatagatt
     5101 ggtacgtagt caaaccagca aatggaaaag ttgtctattc caccccaagg gctttaaaga
     5161 cagcatcttc cggcgtttga taaaaaatca aattaaaggt acccattaat tctgggggta
     5221 cagttccaat atcagcagcg cttatcatag gtacaagtac ttttttcgct ccggcatctt
     5281 ttgctacttg taaacagtta gctaaattat ctaccttaat catagaacca ccaattgtaa
     5341 tatcccctaa aataactaaa cttggtaata caggtttatt taaggaaact gagcataaag
     5401 ctattatagt tgataacgat aatgtttttg tcataccaat gccattcata tcttgaacat
     5461 gaacgacaaa atcttttgtc gtcaaactaa tagactgact aacacttcca taattagctc
     5521 ttaaatatcg gtatgccgaa tccatcgctt ctcttgctcc agcatctgaa ttgatcccag
     5581 tacgttcaaa ctttccattt cccgaagtca tttgcgtttc caatttatag gtacctaatt
     5641 gcccagtttt gccataagat accgtgtaca aatgccctgg cttccctaac ccttctggaa
     5701 tcaaagttcc tccaccttgt tcaggaacag aaacatactc ttctcgctct tcttctaaat
     5761 caatgtatga aaaattgaca tcataaaatt ccattccacc aattttcttt aattgttctt
     5821 ttacacgacg acgcatttca agagcaaatc ttagtatttc ttcgacatca cttttttcaa
     5881 atctaccatc aggatacaac agtttcatca tcccagatac aatccgacga actgcaatcg
     5941 tatcccgctg atttaattga gaaccaagtt tgaaatattt atcaaaacta tcgccaaaac
     6001 taacttttcg aagctcacgc atgacttcag aaaaataatc tgtaatgaag ccatagtcat
     6061 ttgtaaaaaa atctggtcga tatttaggaa tttcccaacc tggtaaataa caatgcatcc
     6121 gatccaaaaa ggctgaatcc gtccccataa cctccggaaa tggctcaaat aaattcgaag
     6181 ttttcaatat tacatcaacg ctttgattaa tatttcctac aaaagccatt gatgctgaag
     6241 cattcttttc ttctttgcct ctagcaaaag atcctgaagc catgtaatct ttcatgattt
     6301 gaacgccatc attatcctta aatttaatcc cagcaacttc gtcaaaagca acacagtccc
     6361 acatacctac aagtcccatt gtacgactag ccatattata gaaaaggttt gctactgttg
     6421 tttgtccacc tgaaacaagg attgagtttg gtgaaatttc tttatataca tgcgatttac
     6481 ctgtaccacg tggtcctagt tcacagagat taaaattatt ttcaattaat ggaataagtc
     6541 ttgctaaatg aagccatttt acccttttat taaattgtgt aggttccata ccaatggacc
     6601 gtagaagtaa gtcaatccat tcatttgtag taaactcctt gcgagccatc ttgtaagcct
     6661 caatgtctaa accaggcatt tggattggat caaccttttt tgcaataaaa gggctattcc
     6721 aacgatcatt ctcatcatac caatattcca tcgaaacaat acaccagatc cccccaccta
     6781 ataatcgatc aaattttttc ggatactctt cactaatttg aacatttttt aatcccaggt
     6841 tagagaattc tgcttcataa caatctttct tttcatttaa atgtacagtt actttatcaa
     6901 taactgtata accacccaat tcacgaattt ttgcttttac tttttctgct tcatcaggac
     6961 gcacatagtt tcgagcaaga atatttttaa cattttccac acctttttca accaatgttt
     7021 catccaatga cgaagcatac ataccaagta aatattccaa tacataaaca ggtacattcg
     7081 caccttcttt aattttcttt gttaaatctt ttctgacaat ctttccggga aagaattcag
     7141 ataactttaa aatttcctgt tgttccacta gaattcctcc atgtgttcag tcattataat
     7201 agcaattaaa gtatatcata cttaaccagt aggcttgata atacaaaaga aaaagtatcc
     7261 acaaaaaatt gtggatacct cgtttttata tatcaaaatc aaatgat
//
LOCUS       DACDHL010000114         7254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDHL010000114 DACDHL010000000
VERSION     DACDHL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.117"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(13..765)
                     /locus_tag="IV228_002536"
     CDS             complement(13..765)
                     /locus_tag="IV228_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8718382.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1129..3795)
                     /locus_tag="IV228_002537"
     CDS             complement(1129..3795)
                     /locus_tag="IV228_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8718383.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4136..5587
                     /gene="cls"
                     /locus_tag="IV228_002538"
     CDS             4136..5587
                     /gene="cls"
                     /locus_tag="IV228_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8718384.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="IV228_002539"
     CDS             complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="IV228_002539"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8718385.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            7025..>7254
                     /locus_tag="IV228_002540"
     CDS             7025..>7254
                     /locus_tag="IV228_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718386.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIE"
BASE COUNT         2121 a         1517 c         1243 g         2373 t
ORIGIN      
        1 aaaaagaacc cttcacgagt cgtgaaaggt tcttttttta tctgcggacc aaaggagcag
       61 tctataggtc agcttagtcg tactaaggat ttctcgtttt tcactgaata gtttgcgaat
      121 gtacctggtc acaacaggat cttttgcgta cgcgtcatag tatagcttca tcaattcttc
      181 agtaattgat tcctgtgtaa aataagtaaa atcttttttc ttcactttga atccttcatc
      241 aattaagtat gtttcaaatt gatccattac aactggcgat gtattaggat cttcttctat
      301 cattcctagt tgcttttcga taggataaaa aacatcatcg attgattcca ctactctgat
      361 tatcacgcac catttgcttg actttttcat caatttttct aattgaataa gatgtaccaa
      421 tgctggattt gctgctgtaa aaaccatttc atatgtctca tttgattgga taaattcttc
      481 taacgactgt ttgataaagg aaacatttgg caactcattt ttttctgctt ctttttttgc
      541 attttctagc attcctgaag aaaaatcgat caaatcatag cgattaattt cttttgctaa
      601 ggaaagaaat ctgccagatc ctccagcaac gtctgctgct gtttcagcag gcaggacttt
      661 ctccgttttc agataatttt ttacatcttc agcaatagtt acttgcgatt cttgctggat
      721 ttcataatat tcttcagcaa attcatccca ttctttttca ttcatttgat ttcctctaat
      781 tttttgttat atgttttcac ttttcggaat aaatcattct cctcttttat ctattctatc
      841 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
      901 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      961 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
     1021 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
     1081 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
     1141 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
     1201 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
     1261 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
     1321 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
     1381 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
     1441 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
     1501 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
     1561 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
     1621 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
     1681 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
     1741 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
     1801 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1861 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1921 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1981 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     2041 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     2101 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     2161 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     2221 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     2281 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     2341 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     2401 gcgttggaag acgatatctg gtccgccttt aacaaacaag taatttttgt tatcaattgt
     2461 gtgtccagta gacattaatt ttcgatctga gtcaaaagga agttctgctt gtcttgggta
     2521 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     2581 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     2641 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     2701 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     2761 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     2821 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2881 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2941 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     3001 gatacctaaa atcaataaag acaatgccaa aatagccaaa cttagttttt tactgaattt
     3061 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     3121 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     3181 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     3241 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     3301 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     3361 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     3421 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     3481 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     3541 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     3601 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     3661 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     3721 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     3781 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3841 aaaaagagac ctaagacaaa tacgcatttg tcttaggtct caccatttca tacaatacca
     3901 gaaaaccatt tggttaactt actgttgata ttatcgcaac aaacgttgcc ggttactccc
     3961 ctaaggagat attcaactgt actaatatcc taccttatta acctacaaaa agcaactact
     4021 ttagcccgat ttacctacat tttcatgaaa ggagtgaaaa gtatattcac cttggtaggc
     4081 ttctttcttc tatcatgcta tactaatgtt attgtatgta aggaggaaca tttttgtggt
     4141 atctagtatt ataaccgccc tttatctatt aaatgcactt attgctttgg tggctatttt
     4201 gatcaaaccc cgagatgtag cagccatttg ggcatggctc ttagtattgt ttgcccttcc
     4261 tggggtgggc tttgtattat atttattttt cggacgtgga ttaacggata agaaaaaatt
     4321 ctatctccga caaagtgact tgaaagaatt agaaaacttt cagtctttta gaggagatac
     4381 cattgaacat tacgatcctg acatgggcga taaagacaaa cagcaatttg ttgacttctt
     4441 ctcttcatta aatcgtatgc cgctgacaag aatgaattct gtcactcttc tcacagacgg
     4501 acaagagaaa ttggattcac tgcttcaaga tctaaaaaaa gccaaacatt cgatccatat
     4561 cgaatattac gcatttgtga cagataatat cggccagcaa gtcttacatg ttttagaaga
     4621 aaaagccgca gaaggcgtgg aagttcgaat attatatgat gcatttggct ctcatggcac
     4681 aaaagcaaaa gatttcaatc gtctaatcaa aaatggtgga catgtccata catttgttac
     4741 ctcacaaagg gcattacttc gtttccgatt gaattaccat gatcaccgaa aaatcgttgt
     4801 gatcgatgga aagattagtt ataccggtgg tttcaatatt gccaatcaat atgtaaatac
     4861 aacaaaaaaa ttcggctatt ggcgcgatac gcatatacgg attttcggtg ccgcttcttc
     4921 tttgctccag cttcgcttct taacagactg gaacgtctcg gtacctgaag aaaaaaaggt
     4981 cggctatcat ttgaattatt tctttaaaaa agcagatcga gatgaatcta agcttgctga
     5041 tacatccatc cagcttgttt caagcggacc gaataacgaa agggaacaaa tcaagctttc
     5101 atttatcaaa ttgattactt ctgctaaaaa acgtgtttgg atacagacac cttaccttgt
     5161 tcctgatgaa agtgtcattg ctgctttaaa aatcgcaact gcctctggtg tagatgtgaa
     5221 aatcatgatt cccaacaaac cggatcatcc ttttatttat cgagcaacac aatattatgc
     5281 tcggcagctg atcaaggaaa atgtacaaat ccttgtctat gagaacggct tcctccatgc
     5341 aaaaacattg ataatggatg atgaaatctg catggtaggt tcagcaaatc aagatattcg
     5401 tagctaccga ttgaattttg aaacaagtgc tgtcatttac gatcctgagt ttttagaaga
     5461 acttgctact cagttcaaag aagatgagac acattgttca tccatgacaa ctgaaacagt
     5521 caaggaaatg tctaactggc tattattcaa gcaacaaatt tctcgattat tttctccaat
     5581 cctataattt ttttattcaa gtagagaaaa atatactaag attaaaggga ctgtgacaaa
     5641 aatcttgtca cagtcccttc ttccgaacaa acggtgttct cctgccaact cctcggtcac
     5701 aagtcacaat attcaacaaa catgagaagc tgttttttga acttgttcct tgtgactcgg
     5761 agctggacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
     5821 taataagtcc aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac
     5881 ttgatgcagg gcaacttttt cacaacttct ttcttttact ctaattcacc agctgcccaa
     5941 tgattgatgg ggccatattt gtgccctaca tttatttctt cacgaattgc acggtttaca
     6001 tagtcttttg ccaatcggat cgcttgttca actgtttttc ctttcgctaa ctcagctgca
     6061 atacatgcag atagcgaatc gccagttccg ttgatccggt cggtaggata aaatggtgca
     6121 tttaaccaaa atgattttcc tgattcgaac aatacgtagt cattgactgt ttcttgctgc
     6181 gcatttttag catgatgtcc tttgatcata acgttttttg cacccatatc ttgcagttct
     6241 tttgctgctt gcacaaaatc ttcttcagct gctaatgtcc gtccagtcag cttttctgct
     6301 tcataaaaat taggtgtcag tacttcggcc aaagggacaa gcttttcttt caagctttgc
     6361 aaagcagatt cttctagtaa aagatttcca tgtttcgtta cgattacggg atccacgact
     6421 aatggtccaa aatcatattt ttggtaattt tttacaactg tctcaatcaa ctttgaatct
     6481 gctagcatac ctgttttagc tgcacgtatt tgaaagtcct tcgcaatcag ttcaaattct
     6541 ttgtcgataa aatctgttgg caatgtcaca cttgctccga ttccgtaaga attacctgca
     6601 acacatgcgg tcatgacaga aacaccgtaa acatttcttg taaagaatgt atgcatatct
     6661 gcttgcatcc ctgcgcttcc atcgctatct gatcctgcga tcgtcattgc ttgtacatac
     6721 tgatcttcca tcaaatcgat tcctccattt ttataaaaag atttttcata atactgttac
     6781 tgtttctatc atatgttttt caaagtatcc ctcagtaaat ataccactac cttttcttgg
     6841 taatcaaatt tatttgaaaa aacatttgca ataaatcaga aaatagtggt cgtttatttg
     6901 tattcgctat tctaataata aataaccact atttgattta tatatatttc agtatccttc
     6961 ttgttttcat ataaccaaag gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa
     7021 aaaaatgaaa cttgaggata aaaaagtcat tttgaagaaa atcaaacgac taccagacca
     7081 aatagatggc tatacgatct gttctttcaa aaaagacagg agtataaaag cttatacgca
     7141 aaacacagaa aaaggaataa tctatttcct acatgaagaa ggttacgata cccaaacctt
     7201 tatgtttact gaaaaaaaag aatttcataa acagatgaaa aaactgattg aaaa
//
LOCUS       DACDHL010000115         6797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDHL010000115 DACDHL010000000
VERSION     DACDHL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.118"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(140..553)
                     /locus_tag="IV228_002541"
     CDS             complement(140..553)
                     /locus_tag="IV228_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8718387.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1009..1494)
                     /locus_tag="IV228_002542"
     CDS             complement(1009..1494)
                     /locus_tag="IV228_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718388.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1633..1848)
                     /locus_tag="IV228_002543"
     CDS             complement(1633..1848)
                     /locus_tag="IV228_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8718389.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2072..2872
                     /locus_tag="IV228_002544"
     CDS             2072..2872
                     /locus_tag="IV228_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8718390.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2891..4759
                     /locus_tag="IV228_002545"
     CDS             2891..4759
                     /locus_tag="IV228_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8718391.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4752..5243
                     /locus_tag="IV228_002546"
     CDS             4752..5243
                     /locus_tag="IV228_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8718392.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5231..5785
                     /gene="srlA"
                     /locus_tag="IV228_002547"
     CDS             5231..5785
                     /gene="srlA"
                     /locus_tag="IV228_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8718393.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5803..>6797
                     /locus_tag="IV228_002548"
     CDS             5803..>6797
                     /locus_tag="IV228_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8718394.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2369 a         1087 c         1324 g         2017 t
ORIGIN      
        1 catatttttt accaagagag atcataacat ctttatcgta aaaacaaaat atttttttgc
       61 tcattttgaa aatttaaaca tactcgttct tatccaaata tataaaaatc gcccctcatt
      121 catcgagagg cgataagttc taatttaact caattttttt tgtttctttt ggctgataag
      181 ctgttttagg taataccaat ttcagcacac cgtttttaaa ggttccagtg attttttctt
      241 catcaacatt aggaagatag aatgaacgtt tgtaagagac cgaatgtctt tctttccgta
      301 catagttgcc atcttcttta tcttctgtat gggactcatg attggcggag atcgtcaaag
      361 tattatctga atattcaact gtcgtgtctt ctttgtcaca gccaggcata tcagcagtta
      421 gtttgtattc tttttcattt tcgactaaat ctacttttgg ataacttgaa acacctaaaa
      481 aatcattgaa tgctggacta aacacgtcat tgaaatcagg gaacatgtcg cgaatgctag
      541 gtacatttgc catttttgat catcctttca ataagtattt tttatttatc cctatttacg
      601 taataactct attttgaaaa aaatgcaagt ataatggtta gctttgtgaa tatttcatct
      661 aatacgatac aaaggctatc tttctaattt gaaaaaggga tcattaaaat aataagaaga
      721 aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa atatagaaaa attgaatcta
      781 gaaaaccgcc cgacaataaa tttcattaac tcagttgacc gaaaatgaag tagataccaa
      841 aaatttatca agagagcaag tagagaagtg agtatattca taagagaaat agctgtaacg
      901 aattattagg ttaatgcaaa tgcttattta tactccgtat aactttctca gaaaaaatga
      961 taccaataat gacttgctag tactgtaaat ccgtataata tcatctaatc aaaatggtgt
     1021 tccatttgca tctgtcatcg taatcgatcc ttcgggtgac actcgataaa aaccaacgct
     1081 tccagatcct ccttgggaac ggatttgttt gctggaagct gtgaacaaat aatctcgatc
     1141 aattttttga taaaagctaa taacaatatc gtcactttgt aattcggaac ggtgggcaat
     1201 aagaaataga agtgctttgt cagagttttc taatggaaga gaaggtacag tgctttcttg
     1261 catttcagat gttgaagaaa tgctaaacga ctgaatactt gattggctat gttgcgaatc
     1321 tacattacca gtagaagtat tagtaatgga agtagtagaa gtacttttag ttgaaccaac
     1381 ctcagttgtt gaaatggctg ctgaagattc tttaacagaa ttctttgttt tatatccaca
     1441 ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa atccctttat gcatgatagc
     1501 tcctcctaaa atagattatc tttattcagt atatcactta aacaaagaaa agcagatggt
     1561 ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg aagataataa cttttttaat
     1621 aattatagct ttctaatatc tttcatcaac ttttctagta atcattggga aaaatttcaa
     1681 gcagtagatc gcacaaatac ctacgacgat ataaatgatc ttcgcaaaaa tagttgttga
     1741 accaccagca atcatagcta ctaaatcaag ttcgaacaag cctaccaaca gccagttcaa
     1801 ccctcccaca atcaataaag ttaatgcaat agcatctaaa gttttcatta gaattctcct
     1861 ttcttaaatg ataaaacaat tatatatcaa aaagagaaac attgcttata taacgctcag
     1921 gaattttagc aatctatgca aaaagatgaa aatttttttt gctcttccat tagtacacat
     1981 tttttagctg aagctgtaaa tttcatgtct taattttttt tagtgagctt ttatactgga
     2041 tttgtaagaa aaaagaagga gaggatcaca tatgtcggat tggttggata ttgcaggaaa
     2101 agtagtaatt gtaactggag gttcctcagg aattggaaga agtatcgtcg aaaatttgtt
     2161 gaagcagaat gcacaagtag cgaattttga tgtaactgaa tgccgtatac agcatgaaaa
     2221 cctactatct ttaaaagtcg atgtgtcttc taaaacggac atagaagaag ggatatataa
     2281 agtaatgaaa catttcaaga cgatcgatgg tctagtcaat aatgcaggaa taaatattcc
     2341 ttctcttcta atagatagaa atcatccaaa aagcaagtat gaattatcag aacaagtttt
     2401 tgataagatg atcgctgtca atcaaaaaag tgtatatttg atgagccaag cagttggaag
     2461 aatcttggtg caaaagggat caggagtgat cgtgaatctt tcttctgaat caggattgga
     2521 aggatcagaa ggacagagct gttatgctgc tacaaaagca gcgatgaaca gttttactcg
     2581 ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg gtaggtgttg caccagggat
     2641 actggaagaa accggtttga gaacacaaga atatgaagaa gctctttctt atacgagagg
     2701 tataagcgtc gaacagctga gaaacggtta cagtcgaaca agtacgatcc ctctaggaag
     2761 gagtggtaag ctgcaagaag tggcggatct agtctgctat tatctttcag aaagatcaag
     2821 ttatatcact ggagtcacga ctaatatttc aggaggtaaa acaagaggat aaaggatagg
     2881 ggggacgcac gtgacgctag taaacagatg gtaccaatta ttgaaattac tagtcactca
     2941 caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa accagtaatc aaaccataaa
     3001 aaaagatatt ggggagttga atgaagaaat tgccggtatt gcaaaaatca tccaaaaaaa
     3061 caaacatttt gaacttgaaa tttatgattt tgattttttt gatgagatca tgcaaggaaa
     3121 attgaaaaca gccagtgatt tcaattcggc atccaaaaga atcgcttatt tactaaatag
     3181 gctgatcgaa acagaggatt tcttacgtat ggatgatttg tctgaagaag tcggtgtcag
     3241 cagaggaaca gtggtcaagg atctaaagac attaaaagag atgatcaatg attttgatgt
     3301 aaagattaca ggcacaccaa ataaaggact aagaataata ggagatgagc tagaattacg
     3361 actactttta ttttattttg tttctcccta cttttcagag acgaaaacag aacagtttct
     3421 agaagaaaaa caagcaatca aacattttca gaagaagacg aatgctagta aacaagaaat
     3481 caccctttta acgaaagtta tcggtatttc tttgaataga atctctagta actatctgtt
     3541 gaagaagccc atcaatcgtt acagtgggtg ttttgaatat atccaagaat tcaatgaact
     3601 gatcaatcag ttggaagaaa tctatgaact aactttgagc caatatgaaa aagattttat
     3661 cagttttcct ttgaacatca tcaacacaag tataacgatg ataaggaaag ctaactctga
     3721 tttgcgctta tatttcgacc ccatgatgat gcgtattcga cgattagatc tcattgatct
     3781 ggatgagtcc ttcttatatc aagagatgaa acagcacctc tctttattga taaatcgggt
     3841 aatttttcgt tatcgattga ctgatttatt ttatggtgag atagaaaaaa agtatccttt
     3901 ttcttaccaa atcgctagcg aatgcataca agcattagaa gctatcttgc aacataagat
     3961 ctctaaagta gagatcagct acctaacgtt gtatttggag ttagcatttc gatatcaaaa
     4021 agagcatttt tcctctaaaa aagtagccat tgttcatacg acaggaaaag gaacagcatt
     4081 aatgatccaa agacaaatca aaaggatatt ggggagggat atacaaataa cacatgtttc
     4141 agaagaacaa tatgaaaaag tagatctaaa caaatatttc gctgttttta caacgattcc
     4201 gttaaaaaat gtaaatgaag agacaccggt gatccatttg actaatcttt ttgatgactc
     4261 ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga ttagccgggt ttcattctac
     4321 tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat caagaatatt taaacaaatt
     4381 agtcgctcct ttgatcagag gctattatgt ggatgaagaa tttgaaaaaa ggatcttgaa
     4441 aagagaatat cagcaaagca catttgttaa caaagaattt gcttttcctc acacaaccaa
     4501 ccagctatct caacacattg ttttatcagt agggctaata actgatcaag gtttagcaga
     4561 aacaggagga aatggagtta tttttttatt tgccatacct gaagaaatga cggctgaagt
     4621 tgagaatgaa ttatttgagc tatacgatat cctatttgaa ctcataaaaa tgggaataga
     4681 tgagtttatt gaattagcac cgacacaaga atcattttta aaactaatca aaagtaaagg
     4741 agagagactt catgaatgat atgtatctat tcatagtttt cgcagcttca gcttatgtgc
     4801 tgcaagcagt tatcagttgg aagcaactaa agcatttcaa tcaaacatat agcgaactgc
     4861 gaagaaaagg aaaagtagcg attggtaaac gacctgggaa aatccgttca ggcacgattg
     4921 ttatgctagc tatagacgat catgatcaag ttttagaagt cagaaaaatg caaggggtta
     4981 catctttggc aaaatttcat tcattacctg tctatgcggg tgaagatatc cattacatcg
     5041 atcgttacca tccctcagtt cgagcggaag atcgtttgac acaagcagct ttagaaaatg
     5101 ccagagaagt atacttacga atagaggcag gaaatgaaaa aagtgaacaa ccttcattgc
     5161 cttttattgc tatgctcaat aattttcgga tatggaaaag tacgatcaaa gaaaaaagga
     5221 gtgacaaacg atggatatca taacaaaatt tgctgaggga tttatgaggc tgttccaaac
     5281 aggtgcagaa acattcataa gctggatgac aggaatcgta ccagttgtct tgatgctgat
     5341 ggttgcaatg aatacgctga ttgctttttt aggtgaagac cgagtaacga aagtagccaa
     5401 attatcagcg aaaaatccgc ttactcgtta ccttgtgtta ccgttcttat ctgcttttat
     5461 gttgggaaat cctatgtcat ttacaatggc tcgtttcctt ccagaatact acaaaccaag
     5521 ctattatgct gctcaagctc aattttgtca tacgagcaat ggtgtattcc cccatattaa
     5581 tcctggagaa ttgtttgtat ggatggggat tgctcaaggg atcgaaacct tagggctaaa
     5641 ttcgatggag ctggctatcc gatacttgct tgtgggactg ctgatgaact ttattggtgg
     5701 ttggataact gattttacta ctggatttgt atgcagacag caaggaatcg ttcttagcaa
     5761 aaaagtagag ctttcagttg attagagaaa ggagagtttt aaatgtatgg tgatattaag
     5821 atcgtaagag gaaacggcgg gtatggaggt cctttgatta ttaggccgac aaaagataaa
     5881 cacaagttca tctatgtcac aggtggaggt gagaggccaa tgatcgtaga taaaatagaa
     5941 gaactgacag gtatggagtc tatcaatggg ttcaaaacat caatacctga tagtgaaatc
     6001 gcgcttgcaa tcgttgactg tggagggact cttagatgtg gaatttatcc taaaaaaggg
     6061 ataccaacca tcaatatcgt ttccacagga aaaagtggcc cattagcaca atatatcact
     6121 gaagatattt atgtttcagc agtagggtta gaacaaatac agccactaat ggaaactgat
     6181 gataacaaag aaacagtaaa tactgcatct ccagataaaa agtacaaata tgatacttct
     6241 aaaaagataa ccgagcaaaa agcagagcag caaaactgga tagcaaaaat tggaatgggc
     6301 gcgggaaaag tagttgctac attcaatcaa tcagcaagag aagcagtaga tacgatgatc
     6361 cggacagtga tcccttttat ggctttcgtt tcccttttga ttggtgtgat acaaggttct
     6421 gggatcggta atgtatttgc taacattatg gcacctttag caggaaatgc agttggatta
     6481 acaatcattg gctttatttg ttcactgcca tttttatctc ctttgcttgg tccaggggca
     6541 gttattagcc aagtgattgg aacattgatc ggtgtagaga ttgggaaagg aaatattcct
     6601 ccccaattag cattaccggc actatttgct atcaatactc aaaatgcatg tgattttatt
     6661 ccagtcggtc taggtttgga agaagcaaaa gctgagacag tagaagtagg tgtaccatca
     6721 gttctttatt caagatttct taacggcatc ccacgagtta tcattgcttg ggtagcaagt
     6781 tttggattat atagctg
//
LOCUS       DACDHL010000116         6517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDHL010000116 DACDHL010000000
VERSION     DACDHL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.119"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..826)
                     /locus_tag="IV228_002549"
                     /pseudo
     CDS             complement(53..826)
                     /locus_tag="IV228_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            847..2049
                     /locus_tag="IV228_002550"
     CDS             847..2049
                     /locus_tag="IV228_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAP8718395.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2782..3750
                     /locus_tag="IV228_002551"
     CDS             2782..3750
                     /locus_tag="IV228_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8718396.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3761..4576
                     /locus_tag="IV228_002552"
     CDS             3761..4576
                     /locus_tag="IV228_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8718397.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4644..5354
                     /locus_tag="IV228_002553"
     CDS             4644..5354
                     /locus_tag="IV228_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8718398.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            5390..>6517
                     /locus_tag="IV228_002554"
     CDS             5390..>6517
                     /locus_tag="IV228_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8718399.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2197 a         1001 c         1328 g         1991 t
ORIGIN      
        1 gccaataaaa cttagtccga tatgcggcgc gttctgtgcg ccgcatattt ttttagtttt
       61 caaagatcag accttgttgt aaatagatcc gtgcccgaaa gttataaaag ttgcgataac
      121 caaacgcaat tcgtttaatc agcttgatct tgttatacag cccctcagtg acgccgtttg
      181 agtgggacac cttaaaggca tttgaaatac ccgattggta gcgatggaag atcgtgaatt
      241 tctttttaaa cgactgaggt aacgtagatg aaattgtttt aagggtttct tgaaagccta
      301 ccgtatctcg tttaacgaaa taatagcgta ataattgaca aaaatgatac gcagttcgca
      361 attcttcgtt gtagcttaac agctcattaa cgatttctgt ttgactaatt ggtcgtttaa
      421 acagtcgatg ataatgccgt ttgagtgcct cccatgtcgt ggaatctttg agtaagagtt
      481 tccaatatcg cttgagccga cggtacttct tctgattttc agagtgtgag gttcgaaaac
      541 gattcatgat ctggacacgt aattgattga agctgcgatt gatgtgttgg acaatatgaa
      601 agcggtcagt tacgatctcc gcatttggaa agaccgtttt aagcaactga caatagctgg
      661 cattcatgtc catcactagg tacttgacct ttaaacgggc ctttctagga aagcgcagga
      721 agtattgagt tagtttgtaa agtctgcgat cttcaagggt cgtaaatagc tgattggttg
      781 tcccatcaac acaaataaaa ctcatggaac cagaagtggc aacattgtgt acttttcttt
      841 gcaatcttgt gtacaaaact attgcaaaac acagcgcata agaatcctta caaaacagta
      901 gatgaagcaa cccaattaga tcttgttata gttgttaatc agctcttaac gggttacgat
      961 tcaaaatggg tcaacaccct atacttagat aagtcacttg aatatcagaa cataattcag
     1021 gctttttcac gcacaaatcg tcttaatggt cctgaaaaac cgttcggaac cattcgttat
     1081 tatcgtaaag cacatacgat ggaacagaac attgaagagg ctattgagtt atactcaggc
     1141 aaacaagcaa aagggttatt tgttgaaaaa ttatataata acttagaaaa gttgaacgat
     1201 atattcgaag aaattaagtc cgtatttaat atttctggca tagaaaactt cagtcattta
     1261 ccggaggttc caggggaaat taagcaattt gtaaatcttt ataatcaact aatccattgt
     1321 tatgaatcag cgatgataca gggattcaca tgggcaaaat cgacttatga aatccaggat
     1381 tatgaaccta taacagtagt aatcacagaa tatgaattta atatcttggc acaacgttac
     1441 agcgagatag agagagaacc tagagacgga aatgatagta tagcgtatga cattgattac
     1501 caattacctg aaggtagtcg tagtcgtatt aattatgagt atttaaacac aaacttcaaa
     1561 aaatacatga atataaaaga agaacgtgat tcgaatttaa ttcaatcatt gttaaacgac
     1621 ttacatcgca catttgcgca attaccagtg gagttacagc catatgctga gatgattcta
     1681 aatgatgtaa ataatgggga attagttatc aaagaaggat gggaatttac tgattacctc
     1741 aatgaatatc agcttagtga agaggacgat tttatccaag atttagtgga ttcaaccaat
     1801 attaatgagg cattattacg tgagatgttg gatttacgct taaccgaggt aaacattaat
     1861 gaatatagtc gatttgataa attaaaatca agtgttaacg taggtcattt cagtggatat
     1921 atcgagaaaa atttaggtaa aatggtaata ccaatcaagg taaatatgct aattgacaga
     1981 ttattgcgtg catttttatt agaagatgta ttcgatgtta cagagtacat aaagaattac
     2041 tttaactaga gtacacctac atcttatagt acataaaaaa attcatcttt tttagtttaa
     2101 taattcacca aaaaggtgag taatgccatt tttgaaaatt gagcttgttt tatgtctaca
     2161 tctgtatccg tatataatcc aaaggattat aaactcagaa gagtctttat tttttctgaa
     2221 tataactact gtatcaaaaa caatcgatta tgagacaaaa caaaatgacc tcttttattt
     2281 aagaaaggag gtcatttttt gtatcataaa ttgtattaaa atcaatgtat cactttttaa
     2341 tcaattttaa gaccataaat tgcgtactaa aagaaattct gcaattcaaa ataaagcatt
     2401 ttaagccatt ttcaaattta agtgcaccca tctacccgtc aaatcacttt attttccact
     2461 ccagtagttt cctaattcaa ctgtgtgcaa aacgatttct tctaaacaaa tcaagacaaa
     2521 cttataaacc ttcatcccag caccataatt atttaaatca taggcatttt tcgaaattag
     2581 tatatgtact caagtttcac tgcagattat ttatattttt tagagaaata aatttttcct
     2641 attgacaaac acaaagaaag cggatacaat acaattatat cacatgacgt tatatgacgt
     2701 tatgtaatat ctttacattt aatcagttac ttttattttg gataaaatta acatttttaa
     2761 aattttaagg aggaaatgtt gatggatgta gcaaaaacgg ttaacgcaat tgtagaaaaa
     2821 aatgatggag agatttctgg aatttatttg gtggcttgtg gcggttcgct ggtagatatg
     2881 tatgtctcag attattttct taaagccgaa gcatcgaagt gtattacagg cttatataca
     2941 gcgaacgaat ttaaccatgt aaccccaaag caattagatg ataaatctgt tgtcattcta
     3001 tgttctcatg gaggaaatac agcagaaacg gtaaaagcag gagaagtcgc tcaagctaga
     3061 ggagctgtga ctgttggtct gactcataat aaaactgcgg atttattaaa ggtttctgat
     3121 catagtttcc tttacgaatg gggagatgat gcaaaaatag tcaataatcc tatggccatc
     3181 attcttgatt taactgtatc tttggtcaat gttaccgaaa catttgaaca ttacggtaaa
     3241 ttcagagaag gcatgaaggt tatcgatacc gtaattgaaa atggcattaa gcaggtgcag
     3301 gatcgtatta aagtgtttgc tgataagtac aaagatgaaa caatgtttta tatcttaggt
     3361 agtggaccat catatggaca tgcttatggt ttttcaattt gctctttgat ggaaatgcaa
     3421 tggcttgatt caacttctgt tcattctgga gaattcttcc atggaccttt tgaagtaaca
     3481 gacaaagata cgaattttat tctcttgatg agcattggac gcactcgtgc tttagatgaa
     3541 cgggcaaaac gattccttga taaatatgca gaaagagtag aaatcgttga tgcaaaggaa
     3601 ctaggattgg atatgatatc agatgaagtt tctgaattct tcaatcccat tttattctac
     3661 agtattcttt cagaatatcg ttcagcttta gcagatgcac gcaatcatga tttagatgtt
     3721 cgtcgctata tggggaaagt ggagtactag gaggattaaa atgaaggtat taggcttagg
     3781 tgataacgtc gtcgataaat atgaaaatct gggaattatg tatcctggag gaaattcatt
     3841 gaattttgca gtttttgcaa aaaaactggg acatgaaagt agctttatgg gagtttttgg
     3901 ctcagatgag gaggcagctc atgttttatc agcaataaaa aaaataggac tggacaacag
     3961 tcactctcgc tttgaaaaag gcgagaatgg ctgtgccaga gtgaagattg atgagggcga
     4021 ccggattttc cttggcagca attcgggagg tgttactcgt gaatacccga ttcagcttac
     4081 tgaagaagac agagaatatt taaaccagtt tgaattgatt catatgggat tatatagcca
     4141 tgtcaatcat ctattagaag agttacaaaa tgtttctgga aagatttctt atgatttttc
     4201 tgacgacttt acggaagaag aggttcagcg ggcaattgat aaagttgatt ttggcttctt
     4261 ttctatagaa gacttttccg atgaagaagt aaagcagttt ttgatgaagc atttttgtga
     4321 gcgaaatgat gtattgattg caacccgagg agaaaaaacg gctattggtt atgatggagc
     4381 aaagttttat gaagtgaacc ccgtgctaga agaaccagtt gatacaatgg cagcgggaga
     4441 ttctttctta acagctttcc tactttattt tctcgaaggg gacgatatag tcaccgcgat
     4501 gaaaaagggg aatgaatttg ctgggaagtc ttgtctggtt gacggttcgt tcggctttgg
     4561 tattcattac gattgagctt gttttaaatt cctgcatttg ataacctaaa gaaagaaatc
     4621 ccggtaaagt ggaggttaaa aaaatgctgg atacaaatgc acaattacca ttgtacttac
     4681 aattaaaaga aatattaaaa aataaaatca atacagggga gtatcccgag aatacgaaaa
     4741 ttcccgctga atcagagctg atagaaagtt atgaggtcag ccgaatcaca gtgagaaaag
     4801 cagtagaaga actggtagaa gaagggtacc tacttaaaaa acaagggaaa ggaacttttg
     4861 tcaaccggca taaagttttt cgtaaaattg aatatgttat gggtttcagt gagagttgta
     4921 aatctaatgg ttttacaccg actagcattg ttttggaacg caagatcatt ccagcaactg
     4981 aagggatggc aaaaaaactc gatatcgctc aaggtgatga tgttttatat atccagagaa
     5041 agcgaatggc ggataacatc cccattatgt tagaaaacaa ttatttttct tatgaaagat
     5101 atagtttctt aatggaggaa gagctgactg attctttgta tgaagtactt aaagtccaag
     5161 gtaccttagc catcaatcct ggggaaactg cacttgaaat tgtcacagct gatgaccaaa
     5221 ttgccaaaat aatggaagta gctgttggga cgcccttttt ctttatggat actttaatca
     5281 acgatcaaaa caacatacca attcatgtag gtaatcaata ttacctggga gaagtatatc
     5341 gtttttactt atgatttcct gttttgattt caaggagtag attttattta tgaagcaagt
     5401 attgcggtca aaaatgattt ttgatggaga gcagccacct ttcaaaggtt atattgtaat
     5461 caatgaaggg atcattgaaa aggtggaaaa caactggaat tacagtgatc tattaaatga
     5521 aaacacggaa ttaattgctt atgatgatca attcgttatg ccagcaattc atgataatca
     5581 tgtatttttc agtggttatt tggctatgaa tgctggcata gatttaagcg atacatcttc
     5641 agctaacgag gctgtagagc gtattaaagc tgtaatgaac cataaaaaag cagatgaacc
     5701 aatttatgct catggctggg atgctgccct gtggaaaact gacccatcag aagagctatt
     5761 gaatgaaata aaccactttg gtccaatcac agcgatcgac aaaaatcgca gccattgttg
     5821 gatgaatgct attgctaagc agacttatgg ttttacagaa aaagaaacca gtgctgaaag
     5881 tcgagtatta ttaattaagg aaatgctagc aaacaaaaag cttttagcag aggtttacgc
     5941 tgaatttgaa gcattactgc tttcaaaagg tgttatatca atgaaagaaa tcgtttttga
     6001 tgacgcagaa tttctttcta atatacctca gaggaaaata cttagtaacc tatatgtgca
     6061 ggcagtccaa gaacctctac agcatgaact tctatatgag tataaggaaa aagttttccc
     6121 tgaaaaggtc cgctttggtg gagtgaagat catggtagat ggtgttgttg cggatagaag
     6181 tggagatatt tacggtgttt atcagtcagg agtagcttca cccgagattg actatgaggc
     6241 aattaaagca gaagttgctg cattcaatga agtagagatt ccttgctgtt tgacaacgga
     6301 aggagataaa gcgggttata aagctgcgtc aattcttgtc gaatttggaa agcggttacc
     6361 tgaaggcgtt tttaattcga ttagtgattt ggaaatggtt actcaaaaga cagcagaaaa
     6421 aatgaacgaa ggacaagtag tagcagaggt atatccacaa attctcggct tgaatcaaac
     6481 gttagaagag gcttacatgt caactgttat aaaaaat
//
LOCUS       DACDHL010000117         6389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDHL010000117 DACDHL010000000
VERSION     DACDHL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.120"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2569
                     /locus_tag="IV228_002555"
     CDS             <1..2569
                     /locus_tag="IV228_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8718400.1"
                     /translation="SHIFSTADSQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHS
                     VRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSG
                     NGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTP
                     AVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVL
                     PFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNK
                     IRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKE
                     GQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHG
                     LIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTK
                     NSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLIN
                     LQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAA
                     TQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKV
                     RYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKI
                     WAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLG
                     SEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLL
                     RREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2751..2990
                     /locus_tag="IV228_002556"
     CDS             2751..2990
                     /locus_tag="IV228_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718401.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            3006..4379
                     /locus_tag="IV228_002557"
     CDS             3006..4379
                     /locus_tag="IV228_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718402.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            4383..6020
                     /locus_tag="IV228_002558"
     CDS             4383..6020
                     /locus_tag="IV228_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718403.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6183..>6389
                     /locus_tag="IV228_002559"
     CDS             6183..>6389
                     /locus_tag="IV228_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718404.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLC"
BASE COUNT         2215 a         1053 c         1296 g         1825 t
ORIGIN      
        1 ttctcatatt ttttctactg cagattctca agcaattatt aactatttag aaaaacagaa
       61 attgtctgaa ggtacagtaa acataactta taatcctatt aaacaagagg tgattacaca
      121 aaaaaagcat tctgtgagag acaatgatat tgaaaaaatt cggtttgtat ttattgatat
      181 tgaccctaaa aggaaagagg gaagtgcaac agatagcgaa aagaaaaagg cagaaaatgt
      241 tatggaacaa gtagaacgtt atttgaaaga aaagagaata gaatcatttg taaaagttga
      301 tagtgggaat ggctaccata tttttcttcc cataaatgaa caaccaaata atcacgagac
      361 gattcttact attcagaatt ttcttcaatt acttcaccga agatttggag tagaagatga
      421 agttgatatt gatacttctg tttataatcc ttcaagatta tgcaaattag ttggtacacc
      481 agcggtcaaa ggaaagtcca caaaagagcg tccgcatcgt ttatctagtt tcctttctat
      541 tcccgataac atagatagaa gtaattgttt taaaattctt gaacagatag ttgaagaaaa
      601 ttccatgatg tcaacctcta gagtaaaaag ccatccatct tttagagaag tgttaccttt
      661 tattcaagca gactataaac aatggttagc tcactatcct gaattaactt ataccataaa
      721 agagggcgat cataaaggag ttaccttact tatatttgac gaatgtccat taagaaaaca
      781 cacgaataat tctaatggtt cttgtttgtc tgttacggct gataataaga ttcgatttca
      841 ttgtctgcat gcttcggata aaaataaaga tattcaagac tttgtaaaaa aatatccgtt
      901 gccaaaacct gtaaatcctt ctgtagaatc attagaatcc aatcaagaat attattttga
      961 ccagtttaaa ctgaatcaag aaggagtatt tttggtgaaa gaaggtcaag aagaaaaagt
     1021 tgcttgtccg atgtttatta agcaaatacg caaaaatatc gaacaaaaag tcattacact
     1081 tgttcttgcc tattatgtcg atcattgttg ggatgaaata gaaatagaag gtggatgttt
     1141 acaaactaat aattttaaac aattagcaaa acatggctta atcattaaag ctagaagaga
     1201 agctgaagtc gttgactttt tacagattca acgggagaat gctccaatct tttttgaaca
     1261 tgaatctctt ggctggaaaa aagaaaatga agcaatgatt tttcagttac aagattcttt
     1321 tggacgtgaa attccttctg tattaactaa aaactcatgc taccaattaa caactaatgg
     1381 tacagaagcg gaatttgacg cattggttca taagtatgtt ttaggaacga acttagaact
     1441 tgcttggtcg attggtattg cttctattgt gttaggctat ttaaacgtta cgaaggctaa
     1501 tacttttttg agtttactaa ttaacctaca gggcaagagc acaacgggta aaaccactac
     1561 gttacacttt attgcctctt tatttggaaa tccaactacg gttcttagaa atatgaatgc
     1621 taccaataaa gccattataa agctagcatc aaacaatcag ggaggtgttc cactaatact
     1681 agatgagtta ggagcagcaa ctcaaaataa ctttagctcg ttgatctatc aattagcttc
     1741 aggagaagaa aggctaagat tagattctaa ttgtcagctt atgccacaag agactttttc
     1801 tgttttgacc cttacctctt ctgaagagcg tttggaaaat tatttcaatg agaaattaac
     1861 tggtttgaaa gttcgatata tcgaattttc tgatatcaag tttacaaagg atgcgaatca
     1921 cgcagagaaa gttaaaagtc tttcttctaa tacttttggt tggggtataa agttatttct
     1981 tttgaaactc tttgaacaag ggattgcaaa aattgaagca acttttgtcg aagctcaaaa
     2041 aaaaatatgg gctgttttac ctgaacattc tttgaagcaa cgcattggta ataatctagc
     2101 tttgatttat agtggagctc tcctaattca agatatactg ggctatcctt tacgattaga
     2161 gcatatacag acggtgctta ttaattcgta cgaacagtta attgtagata ctagtttagg
     2221 aagtgagcaa acggtatttg accgagtgaa aacgttgttg gtgatgaata gccataaatt
     2281 tttaacaaac agtgacaaca aacatacgcc cagtacgatt tggggaaaag tatcaattca
     2341 aaaaaatggg gatattaagg taaatatttt tcctatcccg tttgaggact tgttaaggcg
     2401 agaatttcaa gtaaaggatc taacctatct ttttagacaa ttgcttgatt cgggtaatat
     2461 gtgtgcagaa aaagggagaa gaacgaaacg tattcacatg aatcgtcaag ttcttccaac
     2521 atacgagatt ctattacctt cttcactcaa atcgtatttt cgattgtaat ttagaataaa
     2581 ccaaagtatg aaagtgaggc accatgttat ggaaatattt cttattgatt tagcaaaaca
     2641 actgattact tatctgtatg atgacgagca aacccaaacg gaagtaaatg aagcttgatc
     2701 tatgcttcat ttacttcatt caatcaaaca tttaagaaag gggaattcta atgactaaaa
     2761 ataaaatgac actaaaagca gaggttcttc tgtatattca agaacatttt tctaatcaag
     2821 ccttttttac aaaaccaatt tacttggctt tcgaaataag aggggtatct gctggatcta
     2881 ttggaggtac tcttcaagcg ttgaagaacg aaggatattt agaaaatcac tttgtgcaac
     2941 gttcgttcaa tggaagagtc gcgaaagaat ggtatttagt tcattcatag agaggtgatt
     3001 caagcatgcg tgtggcttgt tatgatcgaa cgtctgttta tgaagaagcg aaaatcagta
     3061 atgacgctca atatgatgca gtgaaagagt atgttttaga gaaatatgaa caaattcctc
     3121 aacgattttc agacaaagga gaatcaggtg gaaaagcgga tcgtccagct tttcgacagc
     3181 ttagagcttg ggtttgtgcg tcagaagaag agggggtgat ctatgtctgg cgttatgatc
     3241 gattggcacg tcaagccaaa atagccttag aatttattga gttgtgccaa caacatcagg
     3301 ttcaagttta ttcgatttct gaacctcttc ctgcttcttt ctcgcacgcg tcaacgaata
     3361 aactatttgt gcaattattg ttcacttttg cagaacaaca acgaaacgtc attattgaaa
     3421 atgttcgtgc cggacttgcc tataaaaagc aacaacggca atatttgtct tctgcggttc
     3481 catatgggta tcgtttagtg aatgggaaaa tccaacaaga agcacatgaa gcaaaagttg
     3541 tccgccatat ctttcaactt tatttgacta aaaaatatgg ctacaaaaag ctttgtcaac
     3601 gattgacgca acaaaagttc ttcttccgtg aacgtccttt tcagccctat catatttata
     3661 gtatcctaaa aaatccattg tactatggcg aaatcaaagg aggatctttg gggaagtatc
     3721 taggaacctt tgaaccgatt ctctcgaaaa caatcttttt acaagcccaa gagattcgcc
     3781 aatcaaggtg tacagctaaa aaagatacgt atccgtactt attacgtcaa aaaattcggt
     3841 gtcccttctg tggtcgtcat ctgagttcga agtatcaatg gaatacaaag aagacgaaga
     3901 cgcttcatta ttatcattgt accgaccgaa gctgccaagg aatctttctt cgggcagaaa
     3961 gggttgagtc agaagtattg gaaaagatac aggctttttt tcaatacgaa ggcgtctatc
     4021 aagccttgat tgcccaattg acgcaacaat tagccgaaaa gcgaaaagat gaaaaacaca
     4081 aagtaatcca acaaaagaaa caagaagcac aatggctcaa ggcatttgaa gagggcaatt
     4141 gttcactgga agaactcaag caacagctgc aagctcttca aaagccgagg tctacttctt
     4201 cgtttctttc actccaacag tgtgaagcaa aagtgaaaga atggcttcaa ctaaaagacc
     4261 tctctagtcg acaattgtta tgtgaccatg tttgtcggat cgaggtaaca aaggaaaaaa
     4321 cagtctgtgg gatttacttc cgacaaatga cacaaaatat tttagaaggg aacgaatgat
     4381 ctatggaaac gacaccaacc aaacgagtcg cattgtatat tcgggtgagt acccaagaac
     4441 aagctttaga aggacatagt attcaagcac aagaacaaaa tggacgacag tttgccgaac
     4501 ggatgagcta tgaagtggta gaagtttatg ccgatgaagg gattagtggg aaatccacaa
     4561 aaaatcggtt cgcctatcaa cgaatgatga acgatgctcg gaaccatcgt tttgatctac
     4621 tagtgatttg gaaattgacc cgtctcggac gcaatatgtt agatgtctta caaaccgtcg
     4681 aggaactgtt gcgatatgat gtggggttac attcgatctc ggaacaattt gacgtgacta
     4741 cttcgacagg aaaactaatg ttacaactcc ttgggagttt cggtgaattt gaacgcaacc
     4801 aaattgcgga gaatgttcag atgtcaatgc aaagcttagt gaaagataaa aagcgttatg
     4861 caggtggacg acgtttaggc tatgtcagtg gtattgacac gaatggtcaa aaacaattgg
     4921 tcattgaacc agaagaggcg aaaattgttc gtctgatcta tgcaaaatat ttaggcggag
     4981 cagggtatcg agcgattgcc aattttttga atcgtcaagg atatcaaaca gtcaaaaaga
     5041 atgctttttc taccacggca gtcaaagata tcttacgcaa taaaatttat gcagggtatt
     5101 tagaatatgc acgctatatc aattgggaaa ccaaacgacg aaagggcaag aacccaaaac
     5161 cgattttagt gaaaggtacc catgaaccga ttatttcgga agaagattat caaaaagtgc
     5221 aagaacgcct tgttttagaa ggcaaacagc ccaaatggaa ccatacaggt gaaaatgtat
     5281 tgacaggctt gttacgttgt ccagaatgtg gggcaccgat ggcagcgagt aatgtcacaa
     5341 atactctaaa ggatggcaca aagaaacgaa ttcggtatta ttcttgttca gtctttcgga
     5401 ataaaggagc aagtgtgtgt catgccaatt cgattcgtgc cgaacaagcg gaaacatttg
     5461 tcgccgaccg attaaaggag atcgttcagc taccagaagt tcttccccga ttggtggcag
     5521 cgttaaatga agaaatagtg cgacaaagcc aaccattgga acaagaactc gtggtgttac
     5581 tggaacgaaa ggaagaattg aagactaaaa ttgaaaaatg ggaagctgct ttggaagata
     5641 gtcctgaatt gtttccgatg ttaaaagacc gcttagacga attgactgaa aaacgacgac
     5701 agttgcacat tcgagaaaat gaaatcttag ggatctttca acaacaagga gagccgattc
     5761 aagtcaaaga tgtccaacgg atattgacta gtttggatcg ttttcttgcc cagagtgaga
     5821 agaagcaagt caaagccttg tatcgtacct ttattgagaa aataaccttt gatcctaact
     5881 ataaggagat attggagatt acaatgaaat tcacgcctgc cgtggtacaa caattgaatg
     5941 agcaatatca aatagccgta tccaaaacaa aggatacggc tatttttgtg ttaaagacac
     6001 cctttacgtt aacgatctag tctaggttat atcaaacaac gttagagggg cgctaacgct
     6061 tctctagcga tttgaaatcg ttagatgagt cgttagggag gtccttatca taaataggga
     6121 tatttattaa tgaatatttg aaatataatt gtatataaga attaaaaaga gtggggatag
     6181 atatgaaaaa gatgggtaag tgtattttat gtgaaaaata cactgaactt actaaagaac
     6241 atgtaccacc taaaaaatca gggaatactg gaggtaaaaa gaccagaact ggtaatttag
     6301 atgatttcct taagagcgat tttacaaaag gtgattttcc gaaggggatt aaaaggaaac
     6361 ctcaaggaaa tgtttattat acgttatgt
//
LOCUS       DACDHL010000118         6341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDHL010000118 DACDHL010000000
VERSION     DACDHL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.121"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(28..315)
                     /locus_tag="IV228_002560"
     CDS             complement(28..315)
                     /locus_tag="IV228_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8718405.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(305..634)
                     /locus_tag="IV228_002561"
     CDS             complement(305..634)
                     /locus_tag="IV228_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8718406.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            939..1268
                     /locus_tag="IV228_002562"
     CDS             939..1268
                     /locus_tag="IV228_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8718407.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(1555..2040)
                     /gene="vanZ"
                     /locus_tag="IV228_002563"
     CDS             complement(1555..2040)
                     /gene="vanZ"
                     /locus_tag="IV228_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP8718408.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2193..3104)
                     /gene="vanY"
                     /locus_tag="IV228_002564"
     CDS             complement(2193..3104)
                     /gene="vanY"
                     /locus_tag="IV228_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8718409.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(3532..4140)
                     /locus_tag="IV228_002565"
     CDS             complement(3532..4140)
                     /locus_tag="IV228_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8718410.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4146..5177)
                     /gene="vanA"
                     /locus_tag="IV228_002566"
     CDS             complement(4146..5177)
                     /gene="vanA"
                     /locus_tag="IV228_002566"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8718411.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(5170..6138)
                     /locus_tag="IV228_002567"
     CDS             complement(5170..6138)
                     /locus_tag="IV228_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8718412.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         1878 a         1334 c         1053 g         2076 t
ORIGIN      
        1 atcctctcaa gttgaaacat tgggttctta cctaatcttt tttaattgtt cataaaaatt
       61 ttcatgcgtt ccacatagta agttaggtat cactgcatta tctttaattt catatgcaac
      121 gcgatatgtc gtacctgcat acttgaatcc aatagcccaa attcctgata aatcacccgt
      181 tttttgttct cccgaatgag gacgaactgc tatctcatca taaattatcg ttaaaaattg
      241 ttcttttaat gttttctctt ttaatttttt tagatacttt attacgtgtt tagaaggtag
      301 aatctcataa gccaatttct gctgccaact cctcttttgt catcggttta gaatttgcaa
      361 cagtatcttc agcaatagat ttaaatgcag aagttaacgc attttttcta tttctaaatt
      421 cttttaagat ctcttgtttg ctataacctt ctgaaagaat atcagccaaa atatcttcac
      481 taaaatcaaa aaattctttt ttaggctcta caaactcata aaggatacca ttctctacaa
      541 gcttagcttc tacttcaaca ccattgggca catcgaattc tttagggacc gttaacataa
      601 tagaatttcc ttgttttctt gtttttgtaa tcatattatt tcctccatta ttttacattt
      661 cgtaattact tagtaattac attgtacgct atttttagta gaaatacaaa ataataaaac
      721 tcctttagaa caattttcaa aggagtttta tttgattagg aaattatgta gaataataat
      781 atgtttttaa cttggaatca aaaacgcact tgcaagtaag tttacttcaa tacatgaaat
      841 tgcttataat agtgtattgg aattaaggta gattgacaaa agagtgatca aataggtata
      901 cttcattcaa ataatcgttt gaatgtgagg tgagatgaat gaaaaaagtt tgccaagtga
      961 ctgtcgtaca taaagaaaaa gtaaatggaa taaaaaataa attggatcaa aaagattttt
     1021 caaacttact tgtgttaggg aaatgtttca gcgatttttc tagaattaaa attttctatg
     1081 ctttagaaac ctataaggaa atgtgcgtct gtgatttagc agaaatactt actgctagtg
     1141 tcgctacaac gtcacaccat ttacgttttt taaaaaaaca tggaatggca aaatcacgtc
     1201 aagatggaaa agtagtttat tactctttag cgaatgaaga catcctttcc gctattcgtg
     1261 ttttttaaag ttatcagaaa atctatctat gaaatcttag tttgggggta ccgtcaggaa
     1321 aatgcggatt tacaacgtta aggaaattta ttaaagagac caattcctca gcactaaaat
     1381 tgataattgg gctttctttg ttactatagt gttaggtaag ctttttactt tatcaccggt
     1441 tttcccctca cttcacacct acgggcgagt ttccccacat cccgttttcc atctaatcca
     1501 attcattcaa tatctttatg tgtatgaaag atcagattgc ttgatttaga catcttacac
     1561 gtaatttatt cttaaatggg tacggtaaac gagcaataat acgagcaaaa gtatacctac
     1621 aaaaataata actctgtcta attttttttg attcatatgc ttattgctta aaccatataa
     1681 tttcagtcca agaaagcctc caacagtatt tgtaattaca tctgttatgt ctgtcgctcc
     1741 aatagcgaag ataaattgaa ttatttcaaa agtaagactt aaaaccagta caaaagcaaa
     1801 cttaggtaaa aatccgattt ctttaaaatt gacattcaaa agcaagccaa atggaataaa
     1861 gattataaca ttatctatca tctctctgaa attcccagta gcagtaaatg gagtcaagtt
     1921 aagacttctt tgatgataat taaatactga taaaatattg tattgtaatt tgaataacac
     1981 taaccagatt agtgtcacta aatataaagc tagcaatcct ctagataata tttttcccaa
     2041 tataatttcc tctaattctt ttgtttaagt tgtatcatta gtatagcaat gtcctagtta
     2101 acgtgggttt taaattaaaa agctaacgtt accccaaaag tgctagtaca ataatgctgt
     2161 tcattaattt cgttggtttc attccgccat ccttacctcc ttgaattagt atgtgttgat
     2221 ccgggaaaca ctgtcacaat tacaccgtct atattgtttc ctgatatctc ataacgaaga
     2281 ttagtcggca catgaatggt ggtattttta gtaacaggat aataaaagat ctcatatttt
     2341 tccccattta cactaacaga aatggttttt tcttctttta ggtaatccat atattcctcg
     2401 agaacgaaat tcttttcttt cataatcgca ctatgtggta aaccaacata gcgaatatgc
     2461 catagttcat attgaattcc tgttaactct gttttgtcct ctggataacg taaaatgaac
     2521 ccgtatttcc aagcattttc ttctatccac tttccttcag gggctcgttc cattttcgtc
     2581 aagcttgatc ctacatctag tgataaacct gaattatgct cactataacc tgctggtaag
     2641 gcatactcag cccccatttc ttggtaaagc acactttgct catcaaagtc tcgatagcca
     2701 ctattaataa taaaatgact aacgccaccc tttacagcat cattgaccat ctctgaaaat
     2761 ttttgtgcta tttcttttga catataaata ttactatcaa gcaacccgta tccatttatt
     2821 aattcgtcat gtttagataa attcacgata tctgacttca cactttcttg gcgaacagga
     2881 tatttactat tgattaatag cagatttcct tgataaacct gttcttctgt aattgttttc
     2941 tcttgggtat tttcagaagt cccactattt tctaaatgtt ctttgggatt ttgatcataa
     3001 ttttgaaatt cgactttttc ctgagaaaac agtgcttcat taacgtagtc ataacctaag
     3061 tatattaaga ataacaataa caataaaaaa aacaacttct tcattttcag tctcctcctt
     3121 aactatatag ttggaagtat gaaatatttc tagaatcttc cccttaaacg gcacaatctc
     3181 gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt
     3241 tactattatc accaatgtag atgaattttc ataagtgata cccatgctga atttaacgag
     3301 gctgaactgc ctgttgacaa tgggcggtgt gaaataccac ccctctatgc gatcagtctt
     3361 attatcaggc tatcttgccg cagcgcacgc agataatgac cgcaatatcg ctctgcctta
     3421 catcttcaat actatctact atatataaat tccctaagaa ccagttacat acgtcgggtt
     3481 tcctgccgaa agagttagct tatatagttt gtccgtgcaa cggttaaaag tttatttaac
     3541 ggggaaatca aaatagctat tggggtatgg ttcgtctctt aatacatagt gccaccattc
     3601 gaggctatat gcttcaaacc cactgttttc catgatggag cgcaaacgtc tgcgattttg
     3661 cgcttcattg catgatattc catttgccgc atgatgagag cgttcatcca taaaatcaaa
     3721 tcggctcccc attggtacaa gctcacccgt gtctaatcga taaagcgtaa gatcaatggc
     3781 actgccgcgg ctatggcttg attttgaagc cacgtatcct tttgaaatca tctcagttcg
     3841 gtcaatattg ggataataac tttcctttgt caggttattt tccggctgtg cagcccattg
     3901 cataaaacag tttacagcac gattaggacg gtaaccgtcc catagaagca atccgtaccc
     3961 ttgggtagca gccagttctt ttgccttcaa aagcgattca gccaactcgt atgtccctac
     4021 aatgcgattt acttcataac cgtcaaccgg ttttccggtg aaattatccc aagtggcata
     4081 tttagcgtcc caacgaacac cgtgtactat ttcatctaaa aaagtaaatc ctatttccat
     4141 gcttatcacc cctttaacgc taatacgatc aagcggtcaa tcagttcggg aagtgcaata
     4201 cctgcagcgg ccatcatacg gggataacga ctgtatgacg tgaaaccggg cagagtattg
     4261 acttcgttca gtacaatgcg gccgttatct tgtaaaaaca tatccacacg ggctagacct
     4321 ctacagccga gcgctttata tatttttttt gccgtttcct gtatccgtcc tcgctcctct
     4381 gctgaaaggt ctgcgggaac ggttataact gcgttttcag agcctttttc cggctcgact
     4441 tcctgatgaa tacgaaagat tccgtactgc agcctgattt ggtccacctc gccaacagct
     4501 aacgcggcac tgtttcccaa taccgcacaa ccgacctcac agcccgaaac agcctgctca
     4561 attaagattt tgctgtcata ttgtcttgcc gattcaattg cgtagtccaa ttcgtccgcg
     4621 ctattgactt ttttcacacc gaaggatgag cctgaacgcg ccggcttaac aaaaacagga
     4681 taggtaaacg tagctgccac cggcctatca tctttattaa taacccaaaa ggcgggagta
     4741 gctatcccag catttttcgc aacgatgtat gtcaacgatt tgtccataca aattgctgag
     4801 ctttgaatat cgcagcctac aaaagggata ccggacaatt caaacagacc ttgtatggat
     4861 ccatcttcac ctgacttgcc atgcaaagct gaaaatgcta catcaacatg gttgatttca
     4921 tattcatggt tctttttaac aagtaatccg tgcatttttt tatccggcga gagtacagct
     4981 gaatagcaat tgtcgttttc ccattccgcg caaggttttt cgcacatttt ccatacacca
     5041 gatttcgtaa ttccaatgta taacggctcg tatttttctt tattaatgtt agcggctatc
     5101 tctattgcag attttaccga tacgtcatgc tcctctgagc aacccccaaa cagtattgca
     5161 acttttattc tattcatgct cctgtctcct ttcaaaatcc aaacagtttt taatggtttt
     5221 ttcaacggta tcacgcaacg cttgctcggt ataataggcc gtatgcggtg tgattatcac
     5281 gttaggcatt ctttgaagtt taagtaaaaa ttgattatca attggttttt gggtgcaatc
     5341 agagtagaaa aactcttcct ctccttccaa tacatccaat gcggcaccgc ccagtttccc
     5401 gttttctaat gctttaacca actcataggt atctacaagt ggaccgcgcc cagtattgat
     5461 aagaaatgct ccttgcttca ttctctgtat ttgttcgtgg ctgataatat agtgcgtatc
     5521 cgtattgagc ggcacatgaa gcgtaacgat atcgctattt tgcagcaact catcaaacgg
     5581 tacatagttt acctctatac ttcggctgcg actataagcc aacactttac atccaaatcc
     5641 tcgcagccgc tcaataaccg ctttgcctat ctggcccgtt cccaccacac caactgtcat
     5701 gtcgctgagt accttgccac ggtcgctgtc caacctgaaa tcatgttttt ccacagagcg
     5761 cacaatcgat tttacgttgc gtactgccat aagaattagc atcatagtat aatcggcaac
     5821 gctatccggc gagtacgcca cattgtcgac agtgatgccc attctcttag cagcagttgt
     5881 atctatatga ttgcagccga tgcttcgggt agaaatatat ttcacaccgg ctctcttcag
     5941 cgcaagaaga atagaggcgg aaatctctga tttatgtccc acactgatac attgattgaa
     6001 aggcgcggat ttggcgttgg attccgacac gttggcgtta attatcgttg ccataacgcc
     6061 aaagcgaggc gaaagagcat ggaatgcatc tgcctcatcc tgctcacatc cataaacagt
     6121 aatgccgatg ttattcataa ttaagaccaa ccctttctgt gaaaggcacc aaataaaacg
     6181 gggatgccaa tggtaatctt gtcaaatctt tctctgaaga acgaaaacgg ctcgttctta
     6241 cggtattaag tctaccgtgt aagaacgagc cgtttcttaa tattaaattg ttaatttcct
     6301 aagaaaaagt aaagataacc ttgagatttt cctaaaaaat t
//
LOCUS       DACDHL010000119         6316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDHL010000119 DACDHL010000000
VERSION     DACDHL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.122"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV228_002568"
     CDS             complement(99..1229)
                     /locus_tag="IV228_002568"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8718413.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IV228_002569"
     CDS             complement(1878..3080)
                     /locus_tag="IV228_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8718414.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IV228_002570"
     CDS             complement(3140..3343)
                     /locus_tag="IV228_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8718415.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IV228_002571"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IV228_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IV228_002572"
     CDS             4880..5692
                     /locus_tag="IV228_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718416.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1875 a         1299 c          996 g         2146 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctct
//
LOCUS       DACDHL010000120         6310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDHL010000120 DACDHL010000000
VERSION     DACDHL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.123"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(103..645)
                     /locus_tag="IV228_002573"
     CDS             complement(103..645)
                     /locus_tag="IV228_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718417.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(958..1509)
                     /locus_tag="IV228_002574"
     CDS             complement(958..1509)
                     /locus_tag="IV228_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8718418.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            complement(1802..3733)
                     /locus_tag="IV228_002575"
     CDS             complement(1802..3733)
                     /locus_tag="IV228_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8718419.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            4032..4346
                     /locus_tag="IV228_002576"
     CDS             4032..4346
                     /locus_tag="IV228_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8718420.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            4388..5296
                     /locus_tag="IV228_002577"
     CDS             4388..5296
                     /locus_tag="IV228_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8718421.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(5398..6009)
                     /locus_tag="IV228_002578"
     CDS             complement(5398..6009)
                     /locus_tag="IV228_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAP8718422.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6026..>6310)
                     /locus_tag="IV228_002579"
     CDS             complement(6026..>6310)
                     /locus_tag="IV228_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8718423.1"
                     /translation="FSNLLVLGKCFSDSSRIKIFYALETYKEMCVCDLAEILTASVAT
                     TSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILSAIRVFLKLSENLSMKS"
BASE COUNT         1970 a         1226 c         1066 g         2048 t
ORIGIN      
        1 aataacccac atgaatgatt tgaaatcgtc ttaaaatgct ttattttata acccaaaata
       61 gaataattac ttcaaaatga cgtcgcatat cactattaat gattaatcga tgacttccat
      121 aatcatttta agttccccgg atgttaaatc ctcgctaaaa atgccagtaa ctttttgtcc
      181 aggtccaatt tcctttgatg ataggtttaa caaagagata gggatataga ttaatccgtt
      241 ttgtttcaaa gacagagcca atattttttt ccttgttcga atagctgcat catagcgaag
      301 agaagagtaa acacctctgc caacaataga aatatgcttc cccaataata aatcaaggct
      361 acctattact tgtaatttct gaaaggtcgt cccatccgtt ttgatttgaa tatcctgtaa
      421 atttattttc ctgtctaagg catcttgaaa aaacaagtgt gtgcctctgc gataatcaat
      481 gccacagagg tgcataaaat tagttgtcga aaaaataatc gctaccgttg agacttctcc
      541 gacaaaggat acttttttat ctacaaaatg ttcttgtaag agatgagcag ccttataaat
      601 cgcgtttaac tgtctggtaa tttgggttgt ctcttttata gacatgcgtt tgtattcttt
      661 ttttgccgcg tgcgaagaat gctccttttt cccaataaaa aaagctgctc aaaagagcag
      721 ccttttgtac aggtttagtt ttggtgcttg ccacccgccg gacccttacg ttccggattt
      781 gtatcgagtt tagttttggt gctcgccacc cgccggaccc ttatgttccg gatttatatc
      841 aagttgacta tgagcctgct ctcatttgta tatataatat accaaaaaaa ggttgatttg
      901 tccaatactg tgggtgatta taaccgtctt actgtgctta tctattatat aatacaatca
      961 aaaacctaac cgaaaattta ataacgtttc actgatttca tcttcattaa ttccgatgta
     1021 gcgtttggta atcttctcgc tgctgtgacc aaagatttcc attaaggtgg ccacgtcttt
     1081 ggttttcttg taatagtggt aaccaaaggt ttttcgtagg gtgtgggtgc cgatatcgtc
     1141 ccttcctaac agcttcgcga ccttttgaaa catctggtag accgtattga cttctaaatg
     1201 gccgccctta gtactgggaa acaagtaagc ctccggatct aagggtgcgg tatagccgtg
     1261 aatcaagtcc tgtaagctgc ttaagtataa aatgcgtgtt ttccctgtct tttgctccac
     1321 gatgcgcggg ttttttgagg taatcacgtc ttttttcttg agtttaacga tgtcggacat
     1381 gcgcagaccg ctgttaattc caattagaaa caagaaaacg tcacgttccg catttttatt
     1441 ccgtcgcagg caaaataaaa aatcattaat ctcctgttgc gaccgtagtg gctgaacatt
     1501 atagcccatg acaatccctc ctttttagtc tgttcgaata catgattata gttattacaa
     1561 cacgaaatcg tgtgtttagg gggagtaaaa aaaaagaacc ctataaggat aggattcttg
     1621 atacatgatt ttatccaatt tcatgtatct tatattcaac ccgcgccaca agagtatggt
     1681 tgatatatca ttttgacttt gaacatctag ataaatataa acaagtaata gagataaaaa
     1741 atgtagtgta atccttaatt atctctatta ctcgattaat ttgaagttgt gcaatcattc
     1801 tttagactgc ctttagcaat cgaagtccat tcaaaataac aaccaaagta cttccttcat
     1861 gaataatgac actgattgca atatccgtta agcctaagaa actaacgaca attaaaaaag
     1921 caacaacggc cattgaaaaa ataatattct gccaaataac acggttcatt ttcgaagaaa
     1981 tattatgaga ttgtaccaat ttagataaat tgttttgcat taaaactaaa tcagatacct
     2041 ctactgccac atcagtccca tctcccatag cgattcccac atctgcatta acaagagcag
     2101 gagcatcatt tacaccgtct ccaaccatgg cggtcacgcc gtatttttct ttttgttcgt
     2161 ctataattct ggatttgtct tccggcatga cattcgcaat cacttcatct attcctaatt
     2221 gtttagcaac cgcttttccc gtcatttctg agtcaccagt aattaatgtg gtgtgtatac
     2281 cttgtttcct aaaatattca atggtttctt tagcatattc actcggaacg tccataaggg
     2341 caataaggcc tataacctct tcatctactg ctacgtatac gactgtctta ccttctgacg
     2401 cccattcatt atttaaacga ctatattcat ctgaaacatc atcaaacgaa gtcggttttc
     2461 ctatacgata atttctccct ttgtaatctc ccgtcaatcc tttaccaatt tggttttcta
     2521 cttcaatagt tagtttgttt ttttgctcaa actttcttag aatagcatct gctaaggggt
     2581 gattggattc tttttcaaga gctactacga tatcaatcat gttttcttcg ttcacggaat
     2641 cagcaaaata ataattggtt acttcaggtt ttcctttggt caaggtgccc gtcttatcaa
     2701 atgcaattgc tttaatatcg gctaattgag acaggtaaga actacctttt gaaaggactc
     2761 cttttttggc taaattagaa gtcgtcgata gcgttgccga gacagtagct gctgctaaag
     2821 cacacggtga agctgcaact aaaaggacca atcctctata gacactttgt gaccatgtcc
     2881 aatcaagtag aaaaggagcc aataacatga atagaggaat ggcaattaaa acaactgtga
     2941 catatttcgg ttcaaatttt tggataatac tcgcagcttt tgtttggtta tcttggttct
     3001 gattcactaa ttgtaaaatt tttgaaaata ctgtatcttt gttttctttg gtgactttca
     3061 tcgtgaaggt acctgttcca ttgattgtac tcccgaagac gccatctcct ttggatttct
     3121 ctttcgggat actttctcca gtaatagacg actcatcaat ggatgtagat ccagacaaaa
     3181 tgacgccatc aataggtact tgatcgccat tcaacacttg aagttgatct cccactttta
     3241 attcactgac atccacattt tttgtactac catcaggcag gattaaccgg gcagtcgttg
     3301 gattcatttc gagtaatttc gttatttctc gtttgcttct tccttctgca taatcttcca
     3361 aaaaatgtgc gccagaaaaa ataagaatca ataatgttcc ttcccaaaaa ttccccatca
     3421 aagaagctcc aattgccgct aatcccatta aaatatgaga attaggtatg aacttttttt
     3481 gtgcttttga attctcaatt gtttctccaa ggccttcaag aagaataacg tgatacccgg
     3541 cactgattgt agcgattgag aacaaaacat tttgtaccaa ctgataatct tcattcaaaa
     3601 atagagcaat cactgctaat gctaaaccaa caaagtacaa aacgactggc attttcccat
     3661 gatcatggtc gtgatcgtgg ttgtgattat gattatgctt ttcttgagac tgttgttctt
     3721 ggatagtttc cacttctatt gcctccctat ataatttata attcctttgt tttccatcat
     3781 ttacttcttg aatcacataa atttctgcta tttctgactc ctttcgttta ttccaaaagt
     3841 aaatcgtaat gaatccacct tacatatgac taattaatca tatgtttgta tactcatttt
     3901 atatcgctat atacattttg tcaagataaa gagaacgcca cttctctttt ataaacaaaa
     3961 gattagcatt gtttcgctca tatggtaata tagatatgaa cggaaggtca tatgaaagga
     4021 ataaaaacac tatggatata aaaacaactt ctccaatcga taaaggatct attctgtcaa
     4081 taagcaaact atttaaggtg attagtgatc ctacacgatt gtcggttctc tttcttttac
     4141 agaaaaaaga gtgtagtgtt ggaaatatcg ctatttctct ggacatggaa cagtccgcta
     4201 tttctcatca attaaaaacg ttgaaagacg cacgcttagt gaaatcaaga agagaaggaa
     4261 aaagtatgct ttacagttta gatgaccttc atgtttttag tattctcgaa caagtcttaa
     4321 ctcatgttaa tgaattagaa aaataaaacc aattagtatg tagcatacaa acttaggagg
     4381 tttttaaatg tcaaaatcag ataaagacca taatcactcg aatcagaatc aaccgaaaaa
     4441 gaatattaaa attgctttct tcataaatct tattttctct ataagcgaat ttttctttgg
     4501 ctttcttttc aatagtgtgt ccattatgtc cgacgcagtt catgatttag gggactctat
     4561 ttcaattgga ttcgcttggt tttttcaagg ttattcagag aaaaaacaag atgaacggtt
     4621 cacttttggc tataaccgct tttctttact gggcgcgctg ataactggcg ttgtgctaat
     4681 tagtggctcg ttttttatga tctatcgtag tgtgccacgt ttgattgacc cacaacccgt
     4741 aaattatagt ggcatgttct ggctttctct cgtagctatt gcattgaatg gatatgctgc
     4801 gtggatttta agcaaaggca catccaaaaa cgagagcatg ttgaacctcc atatgctgga
     4861 agacgtatta gggtggattg gagtcttgat tgtgagtatt ctaatgaact ttaccgaggc
     4921 atatcgtctg gatcctatct tatcgatttt gattgcactt tatatcctct acaaaaccgt
     4981 tcctgaattt ttcaccacaa tgaaaatttt attgaatggt tcgcctgaaa atgtaaacgt
     5041 aaagagcttg gcggactcca ttaaaaatgt tcctgctgta aaagacctct cacattttca
     5101 catttggtca ctggacgggg aagaaaacgc ccttatcatc acggttctta tagacccttc
     5161 agacatagac aaggctaggg aaataaaaga agaaattgca gaagtcgcac acaaatcaag
     5221 cgtagagaat catatcacaa tagaaataac tacaagcaaa gaagagcttg aaaagggata
     5281 ctcccacaac acgtaagata gttctacgct aaaaaatact tcattatttg aaaagcaaaa
     5341 aaggcagcga ttttgattag tcgctgcctc tctttttatc tttcgtgatt atgattttta
     5401 aaaacctagt agcgtttgta tcgtcccgtt ctctatcata ataaaaattc ctaacgcaat
     5461 aaacattata gggacaatga ttcgttcgta tttttctaac gtttcggaaa caaaagaaat
     5521 cttggctaat ttgtaactga tatagcagag aaccgcaaca gagatagcga atacgattaa
     5581 agcaataatg atttctgaaa aagctaacga ggtaaagtat ggaatataaa tacctaaatt
     5641 atcgcctcca gaagcaatcg ttattaaggc aactgtccag aataagcgat tagttcctct
     5701 agattcgagt ttttcaacga cttcctcttc ttcttcctcc tcttcgccaa caaaagcgac
     5761 tctgattcct aagaaaatcg gaattagacc gagaagacca atgatccaat cttgcggaat
     5821 aaaatttagc acataagcag caaacaaact tacggctacc aaaatacctg ttccaagata
     5881 ttgaccccaa aaaatgtgac gaataccttt ttttgtgtgg ctttgagaaa agataatcag
     5941 caagataaac aaataatcaa tactggtaga aatataaaca gcgatagctg aaaaaatact
     6001 ttgtaacaaa ataacttcct ccaaactaag atttcataga tagattttct gataacttta
     6061 aaaaaacacg aatagcggaa aggatgtctt cattcgctaa agaataataa actacttttc
     6121 catcttgacg tgattttgcc attccatgtt tttttaaaaa acgtaaatgg tgtgacgttg
     6181 tagcgacact agcagtaagt atttctgcta aatcacagac acacatttcc ttataggttt
     6241 ctaaagcata gaaaatttta atcctagaag aatcgctgaa acatttccct aacacaagta
     6301 agtttgaaaa
//
LOCUS       DACDHL010000121         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDHL010000121 DACDHL010000000
VERSION     DACDHL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.124"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IV228_002580"
     CDS             725..811
                     /locus_tag="IV228_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8718424.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IV228_002581"
     CDS             complement(932..1621)
                     /locus_tag="IV228_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718425.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IV228_002582"
     CDS             complement(1628..1906)
                     /locus_tag="IV228_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718426.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IV228_002583"
     CDS             complement(1866..2780)
                     /locus_tag="IV228_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8718427.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IV228_002584"
     CDS             complement(2762..3142)
                     /locus_tag="IV228_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8718428.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IV228_002585"
     CDS             complement(3592..3879)
                     /locus_tag="IV228_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8718429.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IV228_002586"
     CDS             complement(3899..4123)
                     /locus_tag="IV228_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8718430.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IV228_002587"
     CDS             complement(4763..5296)
                     /locus_tag="IV228_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8718431.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IV228_002588"
     CDS             complement(5317..6159)
                     /locus_tag="IV228_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8718432.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDHL010000122         6085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDHL010000122 DACDHL010000000
VERSION     DACDHL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.125"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..634
                     /locus_tag="IV228_002589"
     CDS             278..634
                     /locus_tag="IV228_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8718433.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            644..1543
                     /locus_tag="IV228_002590"
     CDS             644..1543
                     /locus_tag="IV228_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8718434.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2060..2791
                     /locus_tag="IV228_002591"
     CDS             2060..2791
                     /locus_tag="IV228_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8718435.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2936..4963
                     /locus_tag="IV228_002592"
     CDS             2936..4963
                     /locus_tag="IV228_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8718436.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNTYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            5071..>6085
                     /locus_tag="IV228_002593"
     CDS             5071..>6085
                     /locus_tag="IV228_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8718437.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYI"
BASE COUNT         2104 a         1006 c         1255 g         1720 t
ORIGIN      
        1 taattctttt tccaatagtt gttaaaggtt ttttattttt tatagtattt tttgttattt
       61 aagaaaggaa taaggttaaa caaataacag gttgcttttt gggtcttttt ccgttcaaga
      121 aactgaacga agaaaaggga aagttgtctc ttttgtgata agagaaatca taataaacga
      181 atgacttact ttccaataaa ttgagaacag tcagaaccag acaaattagt tgcatctgat
      241 gaataaagtg attattctat gacatgaggt gatacagatg aaattagaag tgacaaaaga
      301 agcacaagaa gtattgaaga ataaattaga agcagacgat caacttttat tagatatcga
      361 aaacggtgac ggtccatttg cagaaagcca agtcagttgc caattggata cggcatttcg
      421 attgatcatt gtcaaaaaag atacacaaac agatctaagt ctttattctg tagtggctga
      481 tacaccagtt ggaccaatca aaatcaaaga cagtgcaata ctttacatgg atgatccatc
      541 cacacttgct ttagaaccaa cttataatag tttacaatta aaaggaccaa gcggcttgag
      601 aaaaggcaac ttgcaagttg ttcgaaaaga ctagagggat aaaatgggaa aattggcgat
      661 catcagcgac ctacatgtgg acatcaataa attcggcgaa tcagagctga tgcagctttg
      721 gcaagttttg caaaagcaca agatcactcg gcttcatcta gccggagata cggctaataa
      781 agtagaccgt tgcctagctg ttgtcgaatt tttcgtcaat aaaggattat ctacgacatt
      841 tcatttcggc aatcacgagt tggcagatgt gcccaatgaa cagatgatgg accattttcc
      901 agacccacat tttttaaatg aacggtatat tgctttaaat gagcagaccg ttcttttagg
      961 gatgaatggc tggtacgact atcagttttc cgaattgaaa gattacgagg caatcgtacg
     1021 aatgaagcgt ttgtattggt atgatcggtt catccagcgt gggaaaacag atctcgaggt
     1081 caatgcagaa atgcttgcag ctgcaaaaaa tttgctagac cagctggaag caaaaaaact
     1141 tgaggtgatt ttatccactc atttcgttcc taaacgggca tttatcatgt atcaatctgc
     1201 tccttatgag cgttggaaca aattaaatgc atttttagga tcagaatcat tcggagaatt
     1261 acttgaccgt tattcaaata tcaaacaagt agtttttggc catacgcatc gtcgctttga
     1321 agataaatcc atcggtcaca cgatttacag ctgcagacct tttggttatt attatgaatg
     1381 gcagctgacc cgagattttg tcttgaaaga acagttgcta gacagctatg atcctatgaa
     1441 attacgtgtc ctcttgcgtg cccatcaccc agcttttcag gcctatcaaa cacaacattt
     1501 gcaagaggaa tttgaacaag cgatgacaat cgttccgtat tgagacagca ttagtaatgt
     1561 gtaaaagaaa ataccttgta aaacttaagg ataagtaaga gcagtagatc catttatcgt
     1621 ggatatgctg ctttttttgc ggaaaataat cagcgaatgt ttatttgatc ggatgatttg
     1681 taactcgcag agtactagat gcaagcgtgt attctttttt tgaacgataa tcacaaaaaa
     1741 aaatataata tacatttttt atttaattat aattaatatt gattgaagtc taggcagtca
     1801 agtcacattt tgatagatag gagggggact tacgaattat tttaaaggct atgacagctg
     1861 tatttctaga ttgtgtttgc atcaagttgc aataaaaact agaaatcaaa tcgggctgat
     1921 aatcatttat aactaatcga tacataggac caaatatagg tctatgttct tttatttcat
     1981 aagtatcgta aaacagaatt ttgctatgga ttaatccaat cgactgatat gtttagtaaa
     2041 taaagaaaga ggaatgtcaa tgatgaaaaa agaaatcgta ttaacagcag caatgatgtt
     2101 attcagcatg gtagcaagta cgacttttgt ttctgcaact gaagtatacc caaaggaata
     2161 taacacagaa ggaacgatta catttgaagc aggagatgag ggtgtaactc ctcctgttga
     2221 tccagagaat cctgatccca ataaacctgt cgatccatca gatcctccaa gccctggaac
     2281 aggaggggca ttatcgattg attatggatc aaaattcaaa tttgggactc aaaaaatttc
     2341 tactgcggat aaaacgtatt atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc
     2401 gacatatgtc caagtgacag ataggagaag taccttatct gggtggaaat taagtgtctc
     2461 tcaaccagaa cagtttaaaa ctgccagtgg ggatgaacta gttggagcac agctgaaatt
     2521 tacgaaaggg caggctgttt ctttggttga tccaacatat acgccgcaaa cggtgaattc
     2581 tgaattaacg ttgacaccgg gaggaaacaa tacgttagca attaatgcta aatcaggaac
     2641 aggtgtgggg acttgggtct atcgatttgg tgcaaatgaa aatgaaaatc aagatgcggt
     2701 acagctttcg gtacccggta aatctgtcaa gttagcgcaa caatattcca ctaaattagt
     2761 ttggacatta gaagatacac ctaacaacta gtttactgaa aagaactgtt aaaaataaat
     2821 acgtagtcat attgtcttgc gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt
     2881 tgtttggata cgaatagttc atctaggaat ttaacgatca ggaggaaaag atatattgaa
     2941 aaagaggagg atcgtgctca ttttaagcag tctcttagtg actaatatct ttccgatcat
     3001 agggtactct ttggaattga atcagttgga gcatggaaat agaagagacg aagaaagcaa
     3061 actcacttcg acattcgaca gtttaataga atccaaccag acacaagaaa gcagtgagga
     3121 tttaagtatt tcgagtagtg gagagaccac tgacaacgta gaagctgagg gttcggacaa
     3181 acaaggacta gaagatgcac caatttctgc ggttgagctg cctgacgatc ccaatattat
     3241 ttctatcgat aaaatttttc aaacacctat cggtactagc acgtccatat tggaaaatgg
     3301 gaagttactg cagttgaacc cagcagaaag gtctcaaaga ggagctattt ggtcaaataa
     3361 gaagattagt ttactctcgg attttacatt taaatcttat ttatacttag gaaatcaacg
     3421 agatagagcg ggagatggca tgacctttac attgactggc gatcctagaa tggctagtaa
     3481 tcccagtcaa gttattggat cgccaggtat gggaattggc tcatattcta ctcgttctgg
     3541 ccaaccttac attgcgaatg cgctctcgat tgagtttgat acgtataaaa atagtggttc
     3601 ttctgatcga atggatcgag agataactgc tgataataga gggcatggac atgtcgcttt
     3661 tgtcacgcct aaaccaaata ataatactta ttctggagag catactggcg tgacggtagc
     3721 tcctacattt ctttctaatg gcacatggcg cttgttaata attaaatggg acgcggagca
     3781 aagaagtctt acttatgatt tggaaggtgt cggtcgctct tctcggatta tacctgatct
     3841 cattaggcag tttggttcta cagaagtaaa ttgggggttt acttcttcaa caggtacttt
     3901 ttttcaagaa aatgctgtcg ctgtcacgcg acttccatcg aatgtcacat caacagctga
     3961 tgtaaaagtc aacgatagtg attatggggc catcgctgaa gctggaggaa acgataagat
     4021 aactatcaga aatacattga aggtcgaggg ggattttctt gaatataaga aaatgcagac
     4081 tagtatcgac ctcccacaag aattaactat tcccgagcat atcttgatca atggaaatca
     4141 aattgatgcc aaagatatct tgataaccgg aaatcgagtc acgctaaatc tgacagatta
     4201 ttttattgct ggagatacgt taataatcga attggagaca actttgcagg aagtaattcc
     4261 tgaaaaagta ctcactatgc attttgaata ccttgaaaac gggaatttga ttgagcaatc
     4321 gaatgaaata aacctaaaga tcgcaaagct gaaggagata gatatccatg tttattataa
     4381 agataaagac agtcagattg atattgctga aatgaaaaca atcactggga aaataggaga
     4441 gaagtataaa gaaactccta ttgatattga agggtatgtt tttgactcgg attcagcgaa
     4501 tacagaaggc atcttctccg aaaatacaga ggatatctat ttttattaca gaaaaggcaa
     4561 actttatctc tcaactgctc ctcaatatgt caattttgga aaacataaaa tatccagtag
     4621 accattaagt gaattcggac agttttcagg tgggttgaaa attatagatg aacgggcttt
     4681 tggaagttgg aaattacaat tgaaacaaga gaaagttctt acaaatggag attttgagct
     4741 tccagaagct ctttcctttg ttacctctga aaatacaacg gttataggga catccgctgt
     4801 tacgattttc gaaagtgatc aaaagggtga aagcgatttg tcctctcttt tagattctac
     4861 agctcatcgt ggaatcagaa taaatattcc tgtagaaaac caaagaatcg gaacctttga
     4921 ggggaaacta tcttggatac ttgcagatgt cccaggaaat taacaggaag agaagaatat
     4981 aacaaaatta ttgagtttca agaagggcac aggtaccata ggctcttgtg tcccacagag
     5041 acaagatttt gttatagtct tctcttacat atgattaaag gagcgatttt cttgagacca
     5101 aaacaacaat tatttataac actcctagtt attttagggg cactttttaa caatatatca
     5161 gtgtcagcta gtgcatttaa tttttcagtg acgccgataa cttcagagaa tcaaatcgac
     5221 aagagaaaaa cttacttcga tcttcaactt gatccggatc aggaagtaga agtaaaagcg
     5281 gaattgagaa atgatacaga aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc
     5341 accaattcaa atgtgatggt tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg
     5401 atctttgatc tggtagatta tgtgagctat ccacagatag tgacgctaaa acctaaaagt
     5461 gtccaaaccg ttgcttttca tgtcaggatg ccgaatgagc gatttgatgg tgtattggct
     5521 ggtgggatca cattccaaga aatccagacg gaaaaggacc aaacagaaac taaggatcaa
     5581 agcttatcga ttgagaatga atacacctat attattgctt tgttgatgca acagacactg
     5641 aacgaagttg ctccgaacct tactcttcat gaagttaaac cagatcaaat caatgcaaga
     5701 aatgtcatct tagcaaacgt tcaaaatgat caaaaaacct atatcaatca agtagttatt
     5761 gaaacaaaaa ttacaaaaaa aggacattct gaagttttat atcaagagga aaaagaagga
     5821 ttgcagattg cgccgaatac aaatttttct tttccaactg ctttaaatgg tcaaccgcta
     5881 accccaggtg aatatcacct tacaatgact gttttaggaa atgaaaatga aaatggaaaa
     5941 ttttcgagaa aaaaaggaaa tactacgatc aactatacca atcaatgggt atttgaaaaa
     6001 gattttacaa tagacggaaa agtagccaag gaattgaaca cgacggatgt tacgattaaa
     6061 aaagacaata attggattta tatct
//
LOCUS       DACDHL010000123         6063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDHL010000123 DACDHL010000000
VERSION     DACDHL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.126"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            361..642
                     /locus_tag="IV228_002594"
     CDS             361..642
                     /locus_tag="IV228_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718438.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="IV228_002595"
     CDS             644..1060
                     /locus_tag="IV228_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718439.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="IV228_002596"
     CDS             1682..2836
                     /locus_tag="IV228_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8718440.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="IV228_002597"
     CDS             2830..3252
                     /locus_tag="IV228_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718441.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4422..5390
                     /locus_tag="IV228_002598"
     CDS             4422..5390
                     /locus_tag="IV228_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8718442.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5407..5931
                     /locus_tag="IV228_002599"
     CDS             5407..5931
                     /locus_tag="IV228_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8718443.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT         2284 a         1038 c          995 g         1746 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aacactttag tgagttttgt
     3481 agaaagtacc tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa
     3541 agggtgggat ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata
     3601 tatgctaata atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag
     3661 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca
     3721 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag
     3781 tatataaata tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag
     3841 acaactttag ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat
     3901 aagggattta tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa
     3961 gagttcctta tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag
     4021 agcagagcga tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa
     4081 tgcgcgaatc tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct
     4141 cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac
     4201 tctcccaagg gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg
     4261 tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga
     4321 attagtacat cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa
     4381 cgtgtatctc ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa
     4441 agtatcatca tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt
     4501 tgttcttttc tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat
     4561 tcgagcaatt aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg
     4621 atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg
     4681 gtttgcaacg tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata
     4741 ttccttttgc tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag
     4801 aatgggtgcg ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa
     4861 tgtttcgatt actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact
     4921 ttcatgaatt acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt
     4981 tcaatccaat acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat
     5041 acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa
     5101 aaaaatcaga cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag
     5161 aactagctaa ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca
     5221 aagaacaaaa gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa
     5281 atacccaaaa taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag
     5341 aactaaaaaa agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta
     5401 gattttatga cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca
     5461 gcaattaaca aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac
     5521 aagtttgtga ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac
     5581 gaaaactcaa atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc
     5641 aaccaagttc ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat
     5701 atgcaactac aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa
     5761 caacaactca ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg
     5821 acttatacgg aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg
     5881 atagaaaaac aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagcaa
     5941 tttaacagaa ttaggcagaa gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg
     6001 tatgagggtt accgttggta actcgaagac cgacaactaa ggcgtattgc ctttttattt
     6061 tgt
//
LOCUS       DACDHL010000124         6013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDHL010000124 DACDHL010000000
VERSION     DACDHL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.127"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..958)
                     /locus_tag="IV228_002600"
     CDS             complement(<1..958)
                     /locus_tag="IV228_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8718444.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGES"
     gene            complement(960..2399)
                     /locus_tag="IV228_002601"
     CDS             complement(960..2399)
                     /locus_tag="IV228_002601"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8718445.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2584..4530
                     /locus_tag="IV228_002602"
     CDS             2584..4530
                     /locus_tag="IV228_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8718446.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4794..5666
                     /locus_tag="IV228_002603"
     CDS             4794..5666
                     /locus_tag="IV228_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8718447.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1907 a         1027 c         1078 g         2001 t
ORIGIN      
        1 tactttctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
       61 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      121 ttgttgtcaa ttgtgggaaa aatttctcaa tgaaagaggt accatcgtat ccaataattt
      181 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      241 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
      301 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
      361 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
      421 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
      481 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
      541 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
      601 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
      661 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
      721 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
      781 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
      841 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
      901 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
      961 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1021 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1081 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1141 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1201 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1261 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     1321 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     1381 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     1441 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     1501 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     1561 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     1621 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     1681 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     1741 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     1801 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     1861 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     1921 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     1981 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2041 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2101 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2161 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2221 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     2281 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     2341 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     2401 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     2461 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     2521 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     2581 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     2641 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     2701 aatcaaaaag ctttagacga aaatccagat gtgaaaagca catttaaaat tgatggacaa
     2761 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     2821 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     2881 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     2941 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3001 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3061 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3121 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3181 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3241 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     3301 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     3361 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     3421 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     3481 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     3541 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     3601 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     3661 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     3721 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     3781 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     3841 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     3901 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     3961 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4021 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4081 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4141 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4201 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4261 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     4321 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     4381 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     4441 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     4501 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atggaatttt
     4561 ctttccaaaa tcaaataata tagattaatc aaatatttga gaataacatt ttatttaatc
     4621 ttttcatata tcaattgtat gaaaaattat tagaatttta aaaaatgata aaaattcaaa
     4681 aagaaaaaat agtagttatt tacttgtcat gttactaaat aaaaaaataa tttaaatatg
     4741 atatatagtc atttatttag tgatttcatt ctaaaataaa gggagaaatg tctatgctat
     4801 acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga
     4861 cgatcaagga aagagtgagc tttccgacaa cgacacctga ggaaacaatg cctttagtca
     4921 ttgacttctt caaacaatac caagcagatt tagcagggat tgggattggc tcatttgggc
     4981 ctatcgatat ccatcgcgat tccgccactt atgggtacat tacctcgacc ccgaaattgg
     5041 catggcaaaa ttttgatttc atcggtacca tgaaaaaaga atttccgatc ccgatttctt
     5101 ggacaacaga tgtcaatgcg gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat
     5161 tgagcagtgt cgtttactat acgatcggta cgggtgttgg cggcggtgcc ttacaagaag
     5221 ggcgctttat cgaagggttt agtcatccag aaatggggca tatgctcgtc gtccctcatc
     5281 caaaagatga ctttgctggc agctgtccat ttcatggcaa ctgtttagaa gggatggccg
     5341 ctggacccgc catcgaaaac cgcttaggga aaaaaggaca agaggttgca gaagatgatc
     5401 cttattggga aatcgaagcc tcctatattg ctcaatgtgc ctacaataca accttgatgt
     5461 tttctccaga tgtgatcatt tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa
     5521 acgtccacga cgcatttgca aaattggtca atgggtatgt cgaaacacct gagttaagca
     5581 aatatatcgt gaccccagca ttagaagaca acgctggaac gctcggttgt ttggcattgg
     5641 ctcgagaagc ggtcttacat tcataaacga aaaaaggctc ttcgacattt agtgttggta
     5701 gagaaagata caactgaaaa tttagtcata accagttagg cagagtgctt aactggtttt
     5761 tgttttactt ggaagatcaa gccttgaatg agataaattc ttgctcgaaa gttatggaaa
     5821 tttcgatagc cgtaggccac ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc
     5881 ggatattttt ttggaccttt ttaacaaata ggttctgttg caaagtttta aatctactat
     5941 cagataaggt agaataatag aaaaagatag caggaggaat gacgatgagt cattttaaag
     6001 gaaagcaatt tca
//
LOCUS       DACDHL010000125         5995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDHL010000125 DACDHL010000000
VERSION     DACDHL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.128"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            255..1559
                     /locus_tag="IV228_002604"
     CDS             255..1559
                     /locus_tag="IV228_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8718448.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1655..2524
                     /locus_tag="IV228_002605"
     CDS             1655..2524
                     /locus_tag="IV228_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8718449.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2636..2956)
                     /locus_tag="IV228_002606"
     CDS             complement(2636..2956)
                     /locus_tag="IV228_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8718450.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3110..3505
                     /gene="dhaM"
                     /locus_tag="IV228_002607"
     CDS             3110..3505
                     /gene="dhaM"
                     /locus_tag="IV228_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8718451.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3507..4490
                     /gene="dhaK"
                     /locus_tag="IV228_002608"
     CDS             3507..4490
                     /gene="dhaK"
                     /locus_tag="IV228_002608"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8718452.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            4518..5123
                     /gene="dhaL"
                     /locus_tag="IV228_002609"
     CDS             4518..5123
                     /gene="dhaL"
                     /locus_tag="IV228_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8718453.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            5270..5935
                     /locus_tag="IV228_002610"
     CDS             5270..5935
                     /locus_tag="IV228_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718454.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         2020 a         1091 c         1310 g         1574 t
ORIGIN      
        1 ttcagtttcc tgattacctc atttgtattg actaggtgga acaatcgaaa tctatacaaa
       61 aaagtaaata aatcttttga atatgaagat gaaaaattga aaaaaagaca agaattactt
      121 gaagaagtgt atacggaacg ttttggaaca aaagaagaga gagaaacggt ccgtttttat
      181 tctgtctccg aagagaaaaa tctagatacg acattcattg cagatttgta taaagaaaat
      241 ggggtggaac tgaaatgatc tcaatcaccc atacaggtaa tttattctta gatacttgtt
      301 tgtctattat gtattttttt ctcgtttctt atccgatttt aggcggattt gtatggttta
      361 ttggtgtctg gtgttatgtg tttttataca aacataagca aaaagaatgg gtagatgttc
      421 ccttgagtgt cgaaccgttc atcacgatca tggtgccagc ccataatgaa gaaattgtga
      481 tcgaatatac aatcgagtat ttgatgacga aattgaatta tcacaactat gaggtattag
      541 tgacagacga tggttcaacg gaccaaacac cagaaatatt agctagactg atgaaaaaat
      601 acgctaatct tcgtgtggtt cggatcgaaa aaaataaagg aaaagcccac gcgtttaata
      661 tcggacttgc ctttgcaaaa gggaaattga ttttaagcaa tgatgcagac acggtaccgg
      721 agccagatgc actgattagg tatgtgaatt atttcattcg ccctggtgca agacatattg
      781 ctgcggtcac tgcaaatatg gatgtccaaa atcgtaccaa attgattgca aaatctcaaa
      841 cggttgaatt ctcaagtatc gtgggcatca ttaaacgaac acagtcagct gttttcggtg
      901 gtctttatgc gtacagtgga gcaaatacga tgtatcgaaa agaagccttg atcgatgtgg
      961 gcggattcag acaagatcga gcgacagaag acatcagtat tgcttgggat catcagttaa
     1021 atgactggtt atctgttttt gctcctgaga ttatcttttt catggaagta cctgtgacgt
     1081 tgaagatgct atatcgacac agaaaacgtt gggcaaaagg tggaacagaa gtctggctga
     1141 cgaatttcaa aaaagtgatg ctccatcctt ttaaacatat tggaagaacg atcatcttca
     1201 ttgatcaaac attgagtatt gtctggtcaa tattcttttg tatttccgta gttctttttg
     1261 ctggattaat cgggcattat gtatatcaag gaaactacga acagatttat attactttta
     1321 cattcagttt tgtgttcatc tgctttgaaa tggtcgcagg atttttccag ttgctagctt
     1381 cgctgattgt agatgatcga cgaagaaaat tgaaatacct tcttttcgca ccactatata
     1441 tgcttttgtt ctggatagtg aacgcgataa cgatcgtgac gactttcatt ccagcagtca
     1501 aaacgatttt aggttatgga agcgggacat ggaaaagccc ggaacgtaca aaaaaataaa
     1561 agatgcaccg ttttttagac aaacagcctg ttgtgtttat ggttcttcca aacatttttg
     1621 cttataataa gaaattagtt aaaaagtagt aaggatggga aaagtgatga aaaaaagaat
     1681 gaagccgtta atctggccaa ttgctgtttc cagtattttc ttattgggac agacagcttt
     1741 tgcggaaact tctgaacctg ctcaaacaga accttcaaca accataagct cttctggtgc
     1801 tgaaaacggt gcctcggaaa cagaaatgac ttccaatgta ccgacagata attcaggaga
     1861 aacgatttca tcttcagaag atacatcaat aaatagttct acctcttctg accaaatcga
     1921 tcatttgcaa gacttggaaa ataaaagaaa agaactcgaa gaagcattag aagctgcaaa
     1981 aaacaaattg aaagaagacc aagcagcaat cgatcaagat ttagctgatc ttaaaaataa
     2041 agtgggaaat gaccgtgcac aacttgagaa aaagcttgcc gaagcgaaag caaaagcgga
     2101 agctgtccaa acagatcttg acgggaagat ccaacaagca aaagaattca atcagcaatt
     2161 aaatcaggtt gctcaaacag cttctggaaa gctaagtcaa actcaagcgg atatcacagc
     2221 ggccagtcaa gcaatccacg gaaagatcag cgaagcacaa acagttgcta atgattttga
     2281 agcaaaaaaa gctgacttag cagctagttt ccaacgaata aaagatattt tgcattcggc
     2341 aggagaaaaa tcagtgacct ctggaagcac gacagaaaca gttcagcaaa cgacagagcc
     2401 atccaaatca acaagtcaga ctttgccaaa aacaaatgaa aaaacagctt ctgtcagtta
     2461 ttccttagca ggggcgggtg tgttagtcgc agcagcttac ggaatcatcc gcaaaaagaa
     2521 ataaaaaaga gaggatggga aggtagagta cctcgaagtt agactgaaaa atctaattct
     2581 tgagtgccac tacatgtccc atctctcttt agttattaaa acagatgtcc cctacttatc
     2641 tcgtggagac agctgttcca ctacatgtga ccatcagcat gccattgtcg ctgcctaata
     2701 cttcatagtc catttttacg ccaatgatcg catcagctcc taatgctttt gcacgttcgg
     2761 ccatttcggc aagtgcttct tctcttgcac ccattagttc gttttcatag ctttttgagc
     2821 ggccgccaaa gatatttcgc aaccccgcct ccaagtcttt aaatgcatgg atcccagtaa
     2881 tgacctcacc aaaaacgatc cctttataag ctactacggc ttttccctct acactgttcg
     2941 ttgttgttgt aatcattcga tcactcctct cttttaatta aagtataaca aacttcaaga
     3001 agcactacac atattttccc aaaattagaa atttattata tacttgaatt gttcattaat
     3061 gaaagcgaaa ccaatcgtta gcaactatgt aaaagaaagg aagattcttg tgaaaaaaag
     3121 tattttgctt atttctcata gtcaaaaagt aacagatggt attaaagaaa tgattgagca
     3181 gatgcaaaaa tccgaagagg tgcaaatttt ttctctcggc ggaataaatg gcgagatcgg
     3241 atcagacccg atgaaaatcg tagaagcagt caatgaatca agcgatgcag ctagttattt
     3301 tgtttttgcg gatatcggta gcgcggtatt gaactcggaa ctggcaaaag acatgttgga
     3361 agaagagcag cagcaacgtt atcatctggt ggatgcgcct ttagtggaag gagcttttgc
     3421 agcagccatc actgccggta taacagatga tactcagcaa atcttaacag aagcacaaaa
     3481 tgccggaaag aaaggatggg aataaattga aaaaaatcat gaatgatcca agcaatattg
     3541 tcgaagaaat gctggaagga ttagtaaaaa gctatccaga acttgttcat cgtgtagaat
     3601 cttcccgtgt tgtagccaag aatcagaaag cggaacaagt tggtttggtt tctggtggag
     3661 gaagcggaca tgaaccatcc catgccgggt ttgtagggga agggatgtta agtgcagcgg
     3721 tgcttggtga tgtgtttact tctccaactc cagatcagat tcagacggct attaaggaag
     3781 ccgatagtgg aaaaggagtt ttgctgatcg ttaagaatta tacaggggat gcattgaact
     3841 tcgatatggc aaaggaatta gctgctatgg acgatattga agtagaaagt gtgatcgtag
     3901 atgatgatat cgccgttgaa aatagtacgt atactgctgg aaaacgcgga gttgcaggta
     3961 ctgtccttgt acacaaaatc gtgggcgatg cagcaagaaa cggtgcttcc ctagctgagt
     4021 tgaaagaact gggagaaaag gtcgtacagg caactaagac gatcggtcta gcgcttcgag
     4081 cagcaactgt accagaagta gggaaaccag gattcgaact aggagaagat gagatcgagt
     4141 atggtgttgg tatccacggt gagccaggct atcgtcgaga aaagatgcag ccttctaaag
     4201 tactggcaaa ggaacttgtt actaagattt tagatgatta ctcgaattta ccaaaagaag
     4261 caggtgtact agtcaacgga atgggcggga cgcctttgat ggaacagttc gtgttcatga
     4321 atgatgtttt agctctgttg gaagaaagag gcgtcaatgt agttttccga aaagtcggta
     4381 attttatgac ctcactagat atgcaaggtc tttctctaac actgatcgac ttgacggaga
     4441 cacaatggaa agattcttta gaaagcaatg tacagacgat ttcatggtaa aaaatctcaa
     4501 gataaaaggg tgataatatg aaactaaccg ttcaagaaat ccaagaatgg ctcacgcaat
     4561 ttgcagaggc aatcaatcag aacaaacaat atctcagtga tctagatacc ccgattggcg
     4621 atggagacca tggaaataac atgggaagag gggtttcagc ttatgaagaa gcttttcaag
     4681 ccgatcatcc tgaaacaatc agcgatacat tcaaagtatt ctctatggcg atgatatcga
     4741 aagtcggtgg ggcttctggt ccgttatacg gatctgcatt catgaacatg atgaaagcga
     4801 caaaagaggt cgattctatt gattctcaag aaaaattagg cgaagtgatc gagcagggaa
     4861 cagcgggtat tcaaagccga ggaaaggcag aagctgacga taaaacgatg ttagatgttt
     4921 ggctgccagt cacagaggca ttgaaggcag ggaatttaac aaaagaaatc attgaccagg
     4981 caaaagaaca caccaaagtc ttggtagcta aaaaaggacg agcttcctat ctaggagagc
     5041 gtgcaatcgg acatatcgac ccaggagctg cttctagtgc cattttattt tcaacgcttt
     5101 tagatgtgat caatcaacct taagttcaat aaaactgcgg tgatgctcat cgcagttttt
     5161 tgtttggctg ttcttatgac ttgatgcaga acagcttttt cacaacctct atttgtccat
     5221 atcgcaaaat tcaggtagaa tacaaaagat aacggaaaga ggcgaaacaa tggaacttgg
     5281 aaaatcagat tatcagttgt ttgatcgacc aatctacgca ttcaaacagc tcaaagagag
     5341 ccatccaaca gataagattg aacaaatcaa gaaagaatat aaagaacact ggcaaaaatg
     5401 gaaagagatt cagctccaga cagctgcact tttgcctgat atgtacggca tgagcaaacc
     5461 taaaatcgaa agctggacga atggctggaa tttacgcagt catttttgga gcgcgtaccg
     5521 aagcgaatct cgtcaagacg agaatgcctg tctagcagtt ttgttgaatc aaaaacaata
     5581 ccaaatctat ttgatgtacc aacattataa aagcgaagaa cgatatggta gcattgcagc
     5641 gtataaccag ttgcttgcga tactgaaaga gtggagcaaa acagtcgata tcaaggatta
     5701 ttacatatgg ccgcaaccag aacatgaact tgacgaccac ttagctttgt ctgactattt
     5761 gtccgataca aagaaacaag aagagttgaa aaaagcgatg agagaccgaa cctttcaaat
     5821 gggtaaacta ttcttctatc cgcaagaaat cgaacacgtt gagcaaataa ccgctgaaac
     5881 cttacaagaa ctagcaccac tatatcaaaa gctaggtgcg gaaaaatttc aatagacaac
     5941 acgaaataag caatgcgaaa aacaaacctt actctcggct tgcgagtgaa ggttt
//
LOCUS       DACDHL010000126         5884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDHL010000126 DACDHL010000000
VERSION     DACDHL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.129"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..869
                     /locus_tag="IV228_002611"
     CDS             <1..869
                     /locus_tag="IV228_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8718455.1"
                     /translation="GTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSL
                     ANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNN
                     EKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLY
                     SLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFH
                     YNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTSLLIISLVIYFL
                     KIKNRIIDDYNS"
     gene            882..1889
                     /locus_tag="IV228_002612"
     CDS             882..1889
                     /locus_tag="IV228_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8718456.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1901..2905
                     /gene="neuB"
                     /locus_tag="IV228_002613"
     CDS             1901..2905
                     /gene="neuB"
                     /locus_tag="IV228_002613"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8718457.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2902..4050
                     /gene="neuC"
                     /locus_tag="IV228_002614"
     CDS             2902..4050
                     /gene="neuC"
                     /locus_tag="IV228_002614"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8718458.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            4023..4700
                     /locus_tag="IV228_002615"
     CDS             4023..4700
                     /locus_tag="IV228_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8718459.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            4690..5832
                     /locus_tag="IV228_002616"
     CDS             4690..5832
                     /locus_tag="IV228_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8718460.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
BASE COUNT         2146 a          756 c         1131 g         1851 t
ORIGIN      
        1 gcggaacact gtcatttgtg ttgataggtt tagtaattta tttaagaata atgataaagg
       61 gtgctcattt ttttgataaa aaacagtgga gatacgcttt aaaaatctct ttgccgctaa
      121 tcccacattc cttagcaaat atagttttag cacaattcga tagaattatg attaattctt
      181 atttaggtcc tgctgcgggg ggaatatatt cctacattag taacataggt attattctaa
      241 gcgtaatatg gatgtctact aataacgcct gggttccatg gttctatggt gagatgagta
      301 aaaataatga aaaaaaagtg aagaaagtta gtaactatta tttagtagtc tttactttga
      361 ttacgatgat tgtaatggta gtttcgattg atttagcaaa atttatggct ccgaaagact
      421 attattctgg gttatcttta gtggttccga taaccttagg ctatttcttt cagtttttat
      481 atagtttacc agttaatgct gaattttatg agaaaaaaac atcgtttatt gcgattggta
      541 cagtagcaag tgctatagtt aatatactat taaacatgat tttcatacct aggttaggaa
      601 taattgctgc tggatacaca accgtattta cgtatttttt actttttatt tttcattata
      661 atatcgctaa aaaaattacg aataaacaat tatttgacac aaaaaaaata tgtatcataa
      721 taatttttgt atcaattttt tctttgatac ttcatatttt aatcaatcaa atgttgattc
      781 gttacgtttt gacatcatta ttgataatta gtttagttat atatttttta aagattaaga
      841 atcgaattat agatgactat aattcatagg aggagatata agtgtttaaa ggaaaaacaa
      901 tattagtaac tggcggaact ggttccattg gaagtgaaat tgttcgtcag cttttaaaat
      961 atgaaccaaa agctatcaga atttatagta gaggtgaaga aaaacaattt tatatgcaac
     1021 aagagctctc aaatcacact aatttgagat ttttaattgg tgatatcaga gactctcaaa
     1081 ggattgatta tgcatgtcgt gatgtggata ttatttttca tgctgccgca atgaaacacg
     1141 ttccagcaag tgaatataat cctatggaag ctgtaaaaac aaatgtgatg ggaactcaaa
     1201 atgtaataga tgcagcgatc aaaaataatg taaaacattt tgtaggtatc agtacagata
     1261 aagtagtgag tcctactaac accatgggtg cgacaaagtt attgagtgaa aaattaataa
     1321 tagctgccaa tcagtataaa ggaaaaacag aaacaatatt ttcttgtgta aggtttggaa
     1381 atgtcatggg gtcaagtgga tctgttattc ctctttttag taaacaaata aaaaacaata
     1441 aagatattac tttgacagat gttgatatga cacgatttat gatgtcaata ccggatgctg
     1501 caagattagt tctgtctgct gctatgaatt caaaatttgg agaaacatat gtcttaaaaa
     1561 tgccggcaat caatatttta actttagctc aatcattaat aaaaatatat aatgatacac
     1621 atgatgaaaa atatagaggt gaaataaaag ttgtaggtat caggccagga gaaaaattat
     1681 atgaagagct aatgactaca gacgaaattg aacgctcata tgaaaatgat aaaatgtata
     1741 tcattccacc acactatcat gctactgacg aactgtacaa agattatgaa aaaattaaaa
     1801 tcactgcata ctcttcagat gcaacagaat taatgagtgg acaggaattt attagaatcg
     1861 caaaagagaa taatgtcgtg acttggtaag gagttttatg atgaaatcag aagtatatgt
     1921 aattgcagaa attggtgtaa accataatgg aagtctagat ttagctttag aaagtattga
     1981 taaagctaaa gaatgtggag ctaacgcggt taaatttcaa acatttaaaa cgaataagct
     2041 agtttccaaa aaggctccaa tggcaaaata tcagaaggat aatcttaaag aggacagttc
     2101 tcaatttgaa atgctaaaaa aattagaatt atctcaatct gattttatta gaataaaaga
     2161 ttattgtata caaaataaaa ttgatttttt gtctactcct tttgatgaag aaagtgcaga
     2221 ctttctaaaa caaattggtg tacaaggatt taaaattggc tcaggagatt taacaaattt
     2281 accttttctt agaaaaatag atatgtatgg gttgccagtt ttattatcta caggtatggc
     2341 tgatattcat gaggtcagtg aggctgtaga ttgtttcata gatagtccag ttactatttt
     2401 acattgtact tcaaattatc cggctagttc tgaagatatt aatttattgg cgattaacac
     2461 attaaaagaa atgttcggga tacccgttgg gtactccgat cattcattgg gatatgatgt
     2521 tggtatttgt gctgttgcga tgggtgctaa ggtaattgag aaacatttta ccttggataa
     2581 ggaactacct ggtccagatc ataaagcttc tttagatcct caggaatttg cagattttgt
     2641 aaagcatatt agaaacactg aaatattttt aggtgatggt gttaagcgag caatgccttc
     2701 tgaacttagt acaaaagaag tatcaagaaa aagcctagtg gtcactgaga atttaaatgt
     2761 gggagatatt cttacagaga aatctcttac aataaaacgt cctggaaatg gaatagctcc
     2821 taagttttta tatcaatata ttggcaaaaa agttaaaaga ccaataagtg cagatagtgt
     2881 tctgcttgag gaagatgttg aatgaaaaaa atacttatag taagtggaac acgagctgat
     2941 tatggaatat ataaacctgt tgctgagaat ttactacaag ataattggga tgtatcattt
     3001 gttgtttctg gaatgcattt atcccataaa tatggattta ctaaagaatt gatcttagct
     3061 gacggattta gaattagtgg agaagttcaa agtttgtttt tagagaacaa taagggaaac
     3121 atggctcggt ctgtggcatt agagataaat ggtttcacaa atatatttga acaaactagc
     3181 cctgattttg tattattact aggggataga ggagaaatgt tggcagctgc gatagcttgt
     3241 ttgtatcttg gaatcaaaac agcacatatt cacggaggcg aagtgtctgg tactgtagat
     3301 gaatcaataa gacatgccat aacgaaaata gcatctgttc attttgctgc tacggaaaaa
     3361 agcaaagagc gtttaataaa aatgggggaa gattcttgga gagtttttcc cgtcggagct
     3421 cctagaatag atactatttt aaataataga ttacctagtt ttagagatat aaaagaaaaa
     3481 tatcatctaa aatttgaaaa gaaaaaatac gttttgcaag tatttcatcc agttgtaacc
     3541 gaactagaaa atatagaaaa acaaatttta gctttgatca attcaatgga acaacaatct
     3601 caaccaatta tttgcatttt acctaatagt gatgctggta gcgaaattat cagaaaaatg
     3661 taccagaaat atttaaaaac ggatattatt tatattacga atttagagcc ggaggaatac
     3721 ctgactatat taaaagagtg caaatttatg atcggaaatt cttcttctgg gataattgaa
     3781 gctgcatcat tcaaaatacc agtaataaat ttaggaacca gacaaaatgg acgagaacaa
     3841 tctaaaaata ttataaatgc aaatctaaat gaaaatgaaa ttgaaactgc tttgaataaa
     3901 atacaagatc ctcagtttat aaaaagcttg aaaaatgtta agaacatcta cggtgacggc
     3961 aacagttcta gaagaataac aaaaacattg aatcaaatat tgaatgatgg ggaagatatt
     4021 aagtggattc aaaaacaaat agcttattaa aatatactag tgatccatac attattattg
     4081 gggctggcgg acacgcaaaa gtcattattg aaaccttaag attagaaaat aagttaatat
     4141 atggattttt tgatgataag caaaataaaa tgcaagatct gaaatatctt gggaaaataa
     4201 acgaattgaa tagctgggcg gatagcaaaa aaaaaatcta taaatttatt gtagctattg
     4261 gtgactgtgt agtaagaaaa aagattataa aaaaattgaa tatgccaagt gagcaatatg
     4321 gaatagctgt tcatccaagt gctatagtgg caaaagaaat ttcaattggt tatggaactg
     4381 ttatttttgc gaatgctgta attaacccag atgctgtaat tggagagcat gctataataa
     4441 acacaggtag cattgttgag catgattgta gaattaataa ctatgttcat ttatctccag
     4501 gtgtatgctt agcgggtgga gtacatgtag gtgtaggaac tcaaatagga attggttctc
     4561 aatgtatcca gatgaagaaa attggatctt ggtgtatgat aggcgcaggg agcactattg
     4621 ttaaggatat tccctcacat tccttagctt atggcaatcc agctaaaatt aagaaagaag
     4681 gaattaattt tgaattctag aattttatta tcaagtcctc atatgggagg aacagaagaa
     4741 aaatatgttg ctaaggcttt taaagataat tggatagcgc cattaggtga aaatgtggat
     4801 aaatttgaag aaagtattac aaaatatact ggttctaagg gcggagcttc tgcgttaagt
     4861 tcaggtacag ctgccataca tcttgcctta agactgctta atgttggaaa aaacgatatt
     4921 gtctttgctc cttcatttac ttttatagct acagtaaatc ctattttata tcaaggggct
     4981 gtaccagtgt tcattgactc agaacctaat acatggaata tgagccctgt tgcactagag
     5041 agcgcattag aagaagctga aagaaacaac caattaccta aggcaatcat cgtagtgaat
     5101 ttatatggtc aaatggctga ttatgaacga attttagcta ttgcaaacca atttaatgtt
     5161 ccagtgatag aagatgcagc ggaatcgtta ggttctactt ataagggaat ttcgtcaggc
     5221 ttatttggga caattgggat ctattcattt aacggtaata agatcatcac aacctcaggt
     5281 ggaggagctc tagtgtctag tgataaaaaa tatgttgagc attcaagatt tttagcaaca
     5341 caagcaagag atcaagcacc gtattatcaa catagcgaaa taggttataa ttaccgtcta
     5401 agcaatattt ctgccggtat cggacgtgga caaatggaag tattgaatga tagagtagct
     5461 aaacgaagag aaattttcaa tcgatataag gaagcttttt ctggaattga tagaattgaa
     5521 atgatgccag aagtggatgg catggtgcca aatcgttggt tgtctacttt aacgatcaat
     5581 gattataatt ctattgatca agtagttaac gtaataaata aactaaatga aaaaaacatt
     5641 gaaacaagag ctttgtggaa accactacat ttgcaaccag tatttcaagg ggtaaaaatg
     5701 tattaccatg ataataatct tagtgtgtca gaaaagttat ttaatagcgg cttatgctta
     5761 ccttctggaa gtaatatgac agatagtgaa caagaatttg ttattgatga gtttaaaaaa
     5821 atattcaagt aggtgtgata ttttatgaac ataggaatta ttggtttagg ttccatgggg
     5881 aaaa
//
LOCUS       DACDHL010000127         5877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDHL010000127 DACDHL010000000
VERSION     DACDHL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.130"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            641..1375
                     /locus_tag="IV228_002617"
     CDS             641..1375
                     /locus_tag="IV228_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8718461.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IV228_002618"
     CDS             1391..2560
                     /locus_tag="IV228_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8718462.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IV228_002619"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IV228_002619"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8718463.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IV228_002620"
     CDS             3650..4576
                     /locus_tag="IV228_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8718464.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IV228_002621"
     CDS             4595..5845
                     /locus_tag="IV228_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8718465.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1204 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DACDHL010000128         5828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDHL010000128 DACDHL010000000
VERSION     DACDHL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.131"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            196..921
                     /locus_tag="IV228_002622"
     CDS             196..921
                     /locus_tag="IV228_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718466.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IV228_002623"
     CDS             911..1420
                     /locus_tag="IV228_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718467.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IV228_002624"
     CDS             complement(1490..2938)
                     /locus_tag="IV228_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8718468.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IV228_002625"
     CDS             complement(2938..3654)
                     /locus_tag="IV228_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8718469.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IV228_002626"
     CDS             complement(3635..3985)
                     /locus_tag="IV228_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8718470.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IV228_002627"
     CDS             4129..4902
                     /locus_tag="IV228_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8718471.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IV228_002628"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IV228_002628"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1696 a         1136 c         1011 g         1985 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt ttttttaatt attatttgtc aaattaagcg agacaaaact
     5761 ttccatttac gtaactcaaa aaaacttcta atggtgtttg gtaatttagt gattttctag
     5821 ggatatgg
//
LOCUS       DACDHL010000129         5689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDHL010000129 DACDHL010000000
VERSION     DACDHL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.132"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IV228_002629"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IV228_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8718472.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IV228_002630"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IV228_002630"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8718473.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IV228_002631"
     CDS             2591..3376
                     /locus_tag="IV228_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8718474.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IV228_002632"
     CDS             3389..4240
                     /locus_tag="IV228_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8718475.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IV228_002633"
     CDS             complement(4364..4816)
                     /locus_tag="IV228_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718476.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IV228_002634"
     CDS             complement(5281..5649)
                     /locus_tag="IV228_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8718477.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1908 a          924 c         1156 g         1701 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttacca
//
LOCUS       DACDHL010000130         5620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDHL010000130 DACDHL010000000
VERSION     DACDHL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.133"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..408
                     /locus_tag="IV228_002635"
     CDS             <1..408
                     /locus_tag="IV228_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP8718478.1"
                     /translation="IPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVS
                     VTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAM
                     GNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            689..1909
                     /locus_tag="IV228_002636"
     CDS             689..1909
                     /locus_tag="IV228_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8718479.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1906..4626
                     /locus_tag="IV228_002637"
     CDS             1906..4626
                     /locus_tag="IV228_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8718480.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4610..5554
                     /locus_tag="IV228_002638"
     CDS             4610..5554
                     /locus_tag="IV228_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8718481.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2089 a          902 c         1105 g         1524 t
ORIGIN      
        1 atcccttata ataatattga ggaaattata gaaaaggatg ctatcagcat ttacaaattt
       61 tttattttta ttcctaatcg tcaattgaaa gctaatatat ttaaccattt gaaaaagatg
      121 ctaaatgtgt ctgttactga atcggcacct gtaaatattg aaatcgtacc tcaaaatgta
      181 tctaaaggtg tggtgtttga tcgtttggaa tcaatctaca atttaaagaa acctttgaga
      241 atcgcgatcg gagatagttt aaatgactta tctatgtttg aaagtgcaga tattagtttt
      301 gcaatgggaa atagtcatca agaaatcatg aaaaaagctg attatgtcac tgtttcaaat
      361 gataaagacg gagtatctca agcatttgaa agaattctta ccatataaac taattaaggt
      421 agtatagaac atagaattct aaatacggaa aaatggagca taaattccag tacaaatagt
      481 acttcttcag tgtcaaacga agcgaatata acatagatat ttaggattgt gacaaaaatc
      541 atgtcacaat ctttttttcg aataaactta ttttttgaag tcggccattt atcgtaattt
      601 ctttgcataa tacgaacaaa ttttcggtta ttgatgaaaa aaaacattga aaatggtatg
      661 ataaaagaaa aaaaggagga aatttttaat ggtgaaattt atccatgcgg cagatttaca
      721 tatggatcgg tcatttgaag gactgacaac tcttgataaa actgtacagg aaaaactatt
      781 gaagacgaat ctgaccgttt tatcaaatat cattgaacag gcaataataa ataacgttga
      841 ttttgtttta cttgcaggag ataattttca tcaaaatcgt ccttcattaa aaatacagaa
      901 acatttttca gaacagatga aacggttaga aaagaaccat atacctgtat ttataatatt
      961 cggaaatcat gatttctatc aaaaagagcg gtattggttc tcctttccta aaaatgtcca
     1021 tttatttaca tccgaaacgg tagagacaaa aaaaataacg ataaaaagtg gagaaactgt
     1081 ctctctcagt ggatttagtt atcgtcaacc gtggattcaa aaagacaaag tgatggaatt
     1141 tccttctaga gaactcacag attaccatat tgggttgtac cacggggaac ctggcgtctc
     1201 tcaaaaagga aactatgctc ctttccaacc ctcaaagatg caagaaaagg gatacgatta
     1261 ttgggcttta gggcatatcc atgtgccaac ggtcttaaat gaaaagcaaa caattgttta
     1321 tccaggtgcg cctcaaggtc atactaaaaa ggaacaagct tctacatcta ttctactagt
     1381 tgaattaagt aaaggaagtt gccaaataca tccgattaaa gtagcagagg tctattggaa
     1441 gacaaaggaa atatcattga ggaaagcaag aacgacaaaa gaggtcattg ttcacattcg
     1501 tcagcagctt tcaaaagtag aggatggatt tagtttaatt gagataaaat taaaagatta
     1561 tgaccatctc aatacagatg ttttggaacg gatacaatca ggagagctat tagactattt
     1621 gttagaggaa ttctcagata ggataaatga tttctttatt tggcgtattt ctctcataga
     1681 gaatactttt tatacgaaga ccccacttgc tgcttctgct aaattgatgg aacagttatt
     1741 tcaatactat cagacacctc aagatttcca acaaatttta aatgaagtat atagtcatca
     1801 agaagcggct agaatattga gcgaactgcc ggaatatcaa gaagaaacac ttgaaaaagc
     1861 caagcagctg cttaatcaag attttttatt tgaggaggat caggaatgaa gattttaagg
     1921 atcgagattg ctgcttttgg aaaatggcgt caaaaaagtt ttgactttta ttcaggcaat
     1981 cagctgatct atgggggaaa cgaagcagga aaatcaacga tttatcaatt tattcaagca
     2041 atattatttg gtttcccttc aaaaggccgt aaaaaaaagg attatacacc aaaagacggc
     2101 tcagcctatg gcggcaaaat atggcttaaa catcctgtat atggtgaatt tgctgtcgag
     2161 cgctacaagc agcaaaatcg cggaaaatcg aaggtgtggc tgggggatca agtgggaagt
     2221 gatgagttac tagaaaaact gatcgcacct ttgacgaaag agttattcca gcaggtattt
     2281 actttccagc aagagcagtt gatggaacta gatcatttga gagaaaaaga gctacatgat
     2341 gcgctgattt ctttaggcat cacgggcagt tctcaagtat tccaaaagcg gcaagaatat
     2401 tatcagaaag cacaacaatt attcaagaaa aaaggccaaa aattactaat caatcaaaaa
     2461 ttaaatgaat ggaaagagct gcaagaaaaa attcatcaaa aacaagaaca ggaagcttat
     2521 tttggcaagt tgatccaaca agaacaggaa tacgagtcgc aacagcagca gttattggca
     2581 caattaaaag aggcgaacaa acaattttca ttagccagac agcaagaact taatttttct
     2641 ttatatgaag aatggcaatc tctcaaaaaa aacagcacta caggtcttcc tgatgagcaa
     2701 cttcttttag atctggagtc tttctccaaa cggtatcagc aaacaaatga gcgtttggaa
     2761 gaattagaag caacactgga aaagcatagc gggatggatc agcagtcagc cagatattat
     2821 ttttatttag agcaggagca ggaactaaaa gatctccagc aacttcaatt tacagcggaa
     2881 caattaaacg aagaatggca aagattacaa attgagacta gagaaacaaa ttcagaattg
     2941 aagattttgg aacaaaggtg gcattggcag aaaaatccgt taccggaaga atttttatat
     3001 gaagaagaat ggaagcatct tctggctgaa gaaagaagga ttgctgatct agaaagccag
     3061 atttctgtgc gtctccaaat agctaaagaa cagcaagaga tgattgaagc agaagtgaat
     3121 gacttggaag agacctatcc agcattatta caaaatcagt cagagatagc accagtctct
     3181 tcatctaaac cgtatatgct gatggctggc ggagcaataa tcattgcagg tatatttgta
     3241 ccaatgccct tgaaagtgat ccttttacta attggtgcag gcatgattgg ctatttcttc
     3301 tacaaaaggc aaccaaaaat taaaactaaa aatgatttct ctgagataaa aggaatgtgg
     3361 caagaaaaac taggacaact tgatctagcc ttagctcaat tagctgaagc agaagaaaaa
     3421 caaaagagaa ctggagaaga aaaacagatt cttcagcaag aagtatatcg ttttgcggat
     3481 catcggcact taggaaagat ggatacgctc aaactcatga aagaatatgg acaggaagtc
     3541 atagattatc agaaattata tggcgtattt gagcggaaac ataaaagaga acaggaaata
     3601 caagaaaaac tacgacaaat cgaccaaaaa tttgagtttg ttaaagaatg gttaccgata
     3661 caagataaga caatttcaga aaaaatggta ttgctttccc gttttataca agagatggaa
     3721 cagatgaaat ttgccagaag ctatcagcaa aatactttat tgaagcaaga aatcaatcaa
     3781 ttgaaaaaga aacaaaaagt atggatacaa gaatacagcg aacaattgac tcaagcaggc
     3841 ttatcttatc cttcggaagt acctgtcttt ttacaaaaag ccaaacaagc cgaacagcag
     3901 atgaaaagat acaatgaatt atcggaaatg cttactccac tatttcctga agagctgaca
     3961 aaagagaaat tatctgtaag actgcgccaa ctgaaagaaa agcaatccca actccagcaa
     4021 gaagaaaaag aagcaagtga aaatcggcaa cgactccaac ttcaagtaga agagttgcaa
     4081 agagacggca cattggacga actttatcaa caagctagcc ggcaaaaagc aaaattacag
     4141 gaattactgg aggaatgggg agcacttgaa atcgccggga cattcttagg tgatctttca
     4201 acagagatga gtgaaagaca attaccccag ttgttaagag cagcaggtga ttatttaggt
     4261 attttaacaa aaggaaatta tcagaagata ttactgaaaa atgatacact gactttaaca
     4321 gacggatata cagaatttcc catctatgag ttatctacag gtacgaaaga tcagctgatt
     4381 atggcgatga gatttgctta cctctcatta gaagctaacc gttcgatatg cccgatcatc
     4441 attgatgatg gctggcttca ttatgatcat cagcgaaagt atcaattagc tgaactttta
     4501 gctaaatttg gagaaaagta tcaaataatt tgtttttctt ctgataaaga aatggtaagc
     4561 tattaccatg agatgaacca aatcgtaaaa caattagaag gagcaaataa tgaaaaaaat
     4621 tcgtgattta gtaatcgatg agcagtttga acaatttgtc ctgatcaaaa gtgcagatgt
     4681 aagaatcgct aaaaacggaa aaaaatttat tgcttttact ttccaagata cttcaggtac
     4741 tatcgatggt aaatactggg atgcttcgga ggaggaaatc aataaattta cagccggcac
     4801 agtcgttttt ctaaacggta aaagagaggt ttatcaagga aaccctcaag taaagattct
     4861 tcatatgcgc ttaacaaggg tagatgagcc aggagagcct agtttataca tggaaagagc
     4921 ccctttgaag aaagaagaaa tggaagaaga aatcaatcaa ttgttgtttg agatcaccaa
     4981 tgcgcattgg aatcgtattg ttcgctatct acttaataaa tatcaaaaac aattttttga
     5041 gtttccggca gcaaaaagaa accatcatgc atttgccagt ggattagctt atcatacttt
     5101 gacaatgctt cacttaggga aatcaatcgc cgatgaatat cctgagctga atcgagcatt
     5161 gttgtatgct ggtatcattt tgcatgattt aggaaaggtg attgaacttt ccggtcctat
     5221 gtcgacagaa tatacgcttg caggaaattt gatcggtcat ctggtattga ttgacgaaga
     5281 aataactaaa gcctgtcttg cgttgaaaat cagcgaagct gacgaagatg ttctcgtatt
     5341 acgtcatatg gttttagcac accatggctt attagaatat ggctcacctg tacgtccaag
     5401 aataatggaa gcagaaatat tacatcaaat cgacaatatc gatgcttcga tgcagatgat
     5461 gttgacttcg attcgtcaaa cagaaccagg aaaatacacc gatcggattt tcggtatgga
     5521 caaccgaagt ttctatgtac caaaagattt atagcaaaaa tttatttttt tcattcaaaa
     5581 caaaaaaagc gagacataag tcttcgcttt ttttgttttc
//
LOCUS       DACDHL010000131         5568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDHL010000131 DACDHL010000000
VERSION     DACDHL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.134"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(103..2007)
                     /locus_tag="IV228_002639"
     CDS             complement(103..2007)
                     /locus_tag="IV228_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8718482.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            complement(2205..2438)
                     /locus_tag="IV228_002640"
     CDS             complement(2205..2438)
                     /locus_tag="IV228_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAP8718483.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            complement(2585..3439)
                     /locus_tag="IV228_002641"
     CDS             complement(2585..3439)
                     /locus_tag="IV228_002641"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8718484.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            complement(3424..3762)
                     /locus_tag="IV228_002642"
     CDS             complement(3424..3762)
                     /locus_tag="IV228_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718485.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            complement(3990..4607)
                     /locus_tag="IV228_002643"
     CDS             complement(3990..4607)
                     /locus_tag="IV228_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8718486.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            complement(4839..5411)
                     /locus_tag="IV228_002644"
     CDS             complement(4839..5411)
                     /locus_tag="IV228_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8718487.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
BASE COUNT         1566 a         1283 c          945 g         1774 t
ORIGIN      
        1 tcaagaactc aaaaaagcgg aaaatcccag atttaaaaat ctgagtttcc gcttttgtca
       61 tttcaaggat ggactattgt ctcagcctct tctaatttat tcttaagata tttttacttt
      121 ctctgcaggt gctgatactt ctttttgttc ttttcgacct ttcaacttat acttcattaa
      181 tctcatgcca ttgaaaatta ccaccaagat actaccttca tgaaccaaca ttccgataga
      241 catattcatc cattcgctga agaagacgct ggtcaacaag accaacacta ctccaattga
      301 aatcacgata ttttgtttca tattatttgc ggtggctttt actaatccta atgcgtgagg
      361 caagttgctg aagttcgagt tcattaaaac gacatcggat gtttcaatcg ctacgtccgt
      421 tccgcttccc attgcgattc caatgtctgc taaagctaag gacggactat cgttaacgcc
      481 atctccaaca aaggctacaa tttgaccacg ttgttgaagt tttccgatat aagccgattt
      541 gtcttccggc aacatgtagc cgtgagcttc ggtcaaacca agttcgccgg cgaccacatc
      601 aacggttcct tggttatctc ctgaaagaac gactagattt ttcacgccca agtcttttaa
      661 ttcctgcaaa ttagctttca cacccggacg gacttgatcg cgaatgccca tcagtacttt
      721 taattcaccg tcgaccgctg tcaaaacgag agagttccct tgttgttcaa accgtttgac
      781 atctttttgc acttttttgc tgagagtgac attttctttt tccatcaagg ccacattccc
      841 aacagccact ctatgtccag ctacacttga aacgattccg ccgcctttca caacttcagt
      901 tccttcaaca ggataaaagt ttgtttctcc aatttgattt aagaccgctt ttgctaatgg
      961 atgatctgat tcccgttcca cactggccag atagccaagc gtttcagcag gatctttttc
     1021 atacagttca gtcgctgcaa cggttgggtt tccgacagtt aaagtacctg tcttatcaaa
     1081 tacaattgta tccacattac tgaagtcttg aatgacttca ctccctttta aaagaacacc
     1141 gttacgagca ccattcccaa taccggcaac gtttgagaca ggcactccga taactaatgc
     1201 gcctgggcaa cctagaacca agatggtgat tgctaactca atattttgac tgaacgccca
     1261 tacaacaatt gccagaacca agacagctgg tgtgtagtat ttagaaaagc gatcaatgaa
     1321 ccgttccgct tcggatttag aatcttgtgc ttcttccacg agttcaataa tttttccaaa
     1381 tgtggtgtcc tcaccaactc ggtcagctct gatttgaatc gttccgtttt ctaaaatggt
     1441 tcctgcaaaa acttctgcat cagctagctt gttgaccgga atagcttcac ctgtaatgct
     1501 agcttcattg atatgccctt cacccgtaag gactatgcca tctactggaa ctttcgcacc
     1561 cgttttaacg agcaagatat ccccttcatc tatatcatcc acttctactt cttcaaattc
     1621 gccattatcc atttgtttca aggcgctttc gggtgccatt tcagtcaatt ctttgatagc
     1681 agatcgcgtt tggttcaatg ttcgttgttc caagtagtgt ccgaataaga ataagaacgt
     1741 gacaatagcc gattcttcat aattttgaat aaaaagcgca ccaatcgctg caatactgac
     1801 taagacatca atactgatga ctttgacttt cattgcttga aacgcttgaa tggcaattgg
     1861 cgtaatccca aagacagagg cgatgatcaa agaccactct gaaagtccta cgttttctaa
     1921 aacaaagtga ctgaagaaac cgagtacgat taacaatcca ctgatgacgg tgataacatt
     1981 tttcttgctt aatatatatt gttgcatgct ttttccctcc aaatttaatt tctcttttat
     2041 tgataaacac agtataagat aattgcctaa gtaattaatt gactgacatc aagtttagaa
     2101 agttctcttt tatcgtttct aagcacctcg aatcggattg caacaaaaaa agcaatcctg
     2161 gaaaaatagc ttttcttcca gaattgccta gttcatttaa tctgttaaac ggccttgact
     2221 tttgctttaa gaaccggata gcctacgttt tcgatagctt tttgaatctc ttcaatcgaa
     2281 gtgacagctg aatcaaaatt ggttttgact ttgctggagt tgaataatac tttaatgctg
     2341 tctttatcaa taccgttaac tgatttcacg gcaccttcaa ttttttgcat acaacttgga
     2401 caagacaacg tttctaattg caatatagct ttttccataa ttcctatctc ctttatgtgt
     2461 ttttatttta tcggcagcag attaatgaac actgcctttt cctctttaca actgtagtat
     2521 agctcacttt tagcaaacga aaattgacgc atatcaagtt ttgcacttct ttttatcttt
     2581 ttgctcaatt caccatctta atagtctttt tctgcccctt attgccatca gaataataag
     2641 aaattggtgg ataatcttgt ctgcgtataa accatactac aatcgcgcca ataaacaggg
     2701 ccagagacaa ggcttgcgaa acgcggatcc actcgccaat ccataaactg tccgttcgca
     2761 taccttcaat gaagaaccga cccactgagt accacaaaac gtaactcaaa gcgacttctc
     2821 cttgacgcaa aagctgtttt cggttccgta tagtgacgat gaggacaaag cccaacaaac
     2881 tccacaatga ctcgtataaa aaagtcggat ggtagtaggt accattcata ttcatttgtt
     2941 caatgataaa ttcaggtaaa taaagatttt ccaagaattg acgagtaacc ggcccaccat
     3001 gagcttcctg attcataaaa ttgccccaac gaccaatgga ctgggctaac aatacattgg
     3061 gcgctaaaat atcaagaacg agtgccaaag aagtaccttt tttctttgca taccaatata
     3121 gcgtaccccc tcctgcaatc aatcccccat aaatcgcaat gccgccattc caaatcgcaa
     3181 ggatttgtcc tggattttgc agataataac caagttcaaa taacacataa taaagccgag
     3241 cgccgaggag tccaatcgga attgcccata aggccatatc gataatggta tcctcttcca
     3301 gtcctttttt ctttgcttcc ttggtgctca actggagagc cgctaatatg cccgctgcaa
     3361 taatgactcc ataccaatga atcacaacag aaccaatctc caccacatgg ggattcaaaa
     3421 cgcctaatac gaatcccatt ctatttgctc catttcttca atttttcgcg tcattctagt
     3481 catggtgatg ctgaacaaga ttccggctaa gctccatgga aaccaaacag tgaatacact
     3541 tttatctccc tggtcactcg cttcgtaata cgccactttt ccgtgtccga ttacgccggc
     3601 tccttttaca acttcctctt ctttttccat agctaaccta tctgcctcaa caacgttaga
     3661 cgtttttgct tcgccgccaa tcgtgttctt ggtgaaaggg acgacaaaga atgcccccgc
     3721 tgctgacaac gtgatggcaa ctatcaccca aaaacaatgc acattcaccc tacttatttt
     3781 tacgttttgt tcgacaaccc tatcttaaag aaaaataaaa caaaaaaact tgagggttat
     3841 catgtttgtg cttttatttt tccaatgaat gaaggtcgtt ctgtcttaaa aaagccaaaa
     3901 acggacagat ggaagcaaaa gcactgagtt ctgtcccgtt gttaatgaag agcgacagaa
     3961 caccaacgtg tagaaaaaag tgtgtccgat tattcgctgt aaagcaataa atcatctaaa
     4021 tcctgaatct tgatcctttt cccagacagc tgttgaatca atccttcgtc ctccaattct
     4081 ccaaatttgc gactgatggt ctcaggtgtc gttcctaaat aggaagcaat gtcctttttt
     4141 gccatcggca gggtgatggt gggagagttg cccatttcag gttccacatt ttctgccaaa
     4201 aacaaaacta aacgggtaat gacactctcc acggctactt gtgtcgtttg acgttctgaa
     4261 ctttctaacc gtttagacat ttccgccaat agttttaaag aaatagctgg gtattgttcc
     4321 agaaaatctt ggacatcttt ttgctgaatc atacagacaa cggtatctcg ggttgcttcg
     4381 gcatattcct cgtgtactgc atccgggttg aacaaggtcc attctccggt gaaatcacca
     4441 gggttcaaga tccgcactag ctgctctttg ccagagtctg ccaatcgata aatacggatt
     4501 ttcccacgat tgataatata taaagtatcc tctttttctt gtgaacgaaa cagaaactct
     4561 cctttttgat actgtttcgt gtgggctttt tcagcaatgt aattcatcgc gctatcctct
     4621 aagtgattga atattggaac caagcggata caggctgcgt ggtcgccaat ttcatgatgg
     4681 gtatgtttag tcaattcatt ctcccccttt atttattttg tttatattcg ctgcttttat
     4741 tatataagtc tcttactaca aaaaaattga tgcacatcaa gaaaatggaa aaaggactgg
     4801 aaagaattcc aatccctaaa tcagcttcta cttttacctt aatcttcatc ctcatcaaga
     4861 gcgtcatcat cttctaatgc ttctttacca atgaacgctt gcagcatcca aatgttcttg
     4921 tctaagtcat ttttaaaagc aatcaatgta tcttctaaag catcgtcacc ctcgtcttga
     4981 gctaaacgaa tcgcgcgaat agttaggtca cgagtgcttc tgaagtcctc gataatattt
     5041 tcaaccatgt cttctgcttt agcgtattta ttaactgcat cttccgaaag catcgaatat
     5101 ttttcaaatt caaccgttgt cgaagcaggt ttatgcccag aagtaattag acgctccgca
     5161 agtttgtcga accatttttc attttcgtta tacaactctt caaatttttc atgcaacgta
     5221 aagaaattgg ctccttttac ataccagtga tattggtgca gtttcacatg taatgtatgc
     5281 atgttgccta aaagatgatc cgcaatggcc gctgcattaa ttttcgtatg atgaacatgt
     5341 tccttgtgtt cttgttcttc ttgcaatctt tcttggggtg ttctttcttg agtagcttta
     5401 ttttcagtca tgatttattc ctcctataat tttggtttgt ttaaaatagc tttctttgat
     5461 tcctcctttt tctttactga atttagtata ggatagattt ccgttaaaaa aattgacgct
     5521 catcaagaaa attatttctt aatagacgcc aggcttctct attaaatt
//
LOCUS       DACDHL010000132         5473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDHL010000132 DACDHL010000000
VERSION     DACDHL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.135"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(420..493)
                     /locus_tag="IV228_002645"
     tRNA            complement(420..493)
                     /locus_tag="IV228_002645"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(457..459),aa:Ile,seq:cat)
     gene            complement(682..1701)
                     /locus_tag="IV228_002646"
     CDS             complement(682..1701)
                     /locus_tag="IV228_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8718488.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1698..1922)
                     /locus_tag="IV228_002647"
     CDS             complement(1698..1922)
                     /locus_tag="IV228_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8718489.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1919..2212)
                     /locus_tag="IV228_002648"
     CDS             complement(1919..2212)
                     /locus_tag="IV228_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718490.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(2249..2386)
                     /locus_tag="IV228_002649"
     CDS             complement(2249..2386)
                     /locus_tag="IV228_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8718491.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(2388..2819)
                     /locus_tag="IV228_002650"
     CDS             complement(2388..2819)
                     /locus_tag="IV228_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718492.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2833..3327)
                     /locus_tag="IV228_002651"
     CDS             complement(2833..3327)
                     /locus_tag="IV228_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718493.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(3327..3776)
                     /locus_tag="IV228_002652"
     CDS             complement(3327..3776)
                     /locus_tag="IV228_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718494.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTMVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(3780..4397)
                     /locus_tag="IV228_002653"
     CDS             complement(3780..4397)
                     /locus_tag="IV228_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718495.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(4397..5305)
                     /locus_tag="IV228_002654"
     CDS             complement(4397..5305)
                     /locus_tag="IV228_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8718496.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(5318..>5473)
                     /locus_tag="IV228_002655"
     CDS             complement(5318..>5473)
                     /locus_tag="IV228_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8718497.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
BASE COUNT         1566 a         1139 c          831 g         1937 t
ORIGIN      
        1 atgatatcta tattttatag tatcagtgct ataaaataaa aaataagtca ctaattaact
       61 accactccaa ttataagcct tttttctcac tttttttcaa aaatatggta tgctttttaa
      121 tggcttcaaa tataaaagag tttaaagcgt aacacactta tggggcagtg gtttttgggg
      181 aacgctttaa actcttcttt attattatct cacaatataa cccaaatgtc tttctattta
      241 aaaatcaaag taaaactttt caaatataca gaaatataac tatgtgaaac atcctttcat
      301 taatccataa aaggatacat aaaaaagcca ctcatttgag tggcaatgaa gaaaagcttt
      361 agcttgtata atactcttca aaaaattcta acacagaacg attcaaatgg ctacgttaat
      421 gtaccctgta ggactcgaac ctacgaccgg acggttatga gccgtctgct ctgaccaact
      481 gagctaaggg tactggttgt tgccacataa agccataaac aatcaaccag tagaatgtgt
      541 ggcaacaaac ctgttatcgc atagcttgga gtgtgactat tatgggtgat agtgaagata
      601 tgcgataaca tcactatttt atcgaatgat ttttatagtt gtcaatatag ttatgtaatg
      661 ctcctcaacg aggagctatt tttatcgttt aggaatattt aaataccaac gtttgtcatg
      721 gaaatcttgc gcaccgcctt tagtgtttcc ctctggatca ttcgttgccc gcatcatgac
      781 atagactttc ttattaggga agttacgcat gttaaaagat acatgatagc caacatttcc
      841 agaagtatta taagcttgat ttacatctgc tctataaatt ccatcagctc ttactcgagc
      901 taattctttc ccagtattgt aatccataat gaagatatac tcgtatttat agttagcaat
      961 gtgccatcca gctacatgca aatttgcatt ttcgatttct ccaaactgat caatgtgggc
     1021 gtaattcgtt ccatctgtca gtgtaggatt agctgcacca gctcgtgttg gatcaatgac
     1081 aggcttgttt tcagaagttg ttggattttc atcggtaaat ccatgagcta aatcatatgc
     1141 taatttttct ttacttacgc ccatttcaga aagataaccg taaggatctg tatgatcgcc
     1201 ccagatgttt tgtgttaccc ataaatgcga tttgattccc ggttggttat aaggagtgtc
     1261 caacgttaat ggaataccat atttcattgc tgaatctcta gccaattcaa cgtatgcctt
     1321 gtagtttttc tcaaacgttg ctttatcatg tgtgtgttgt aactcaatct gcacaggact
     1381 gttggcatta gcatacgaac cagcaccgta ctgtacataa ccaggttgac cgacttgata
     1441 aacaattccg ccgtctccca caatataagc agtataagcg ctagtccatg aacgttgcat
     1501 atactgcgct tcattgcgtc ctgttgctgt ttcattagcc gtttcatgca gtaaaatata
     1561 ctgattattt gctacttgtg agctaccttc atttgcgccc aaattaaatt cattgttgat
     1621 agtataggca aatccgttaa ttggcaataa aaaaagagcc gttaacaggc tcatcgcagt
     1681 aatagtaatt ttctttttca tttgtttcct ccttcttcgc tttcagccga gaacattttg
     1741 taggttcgat ttgatacacc caacacactc cctaaaaacg cgccaaaacc agtaatgatg
     1801 acaacacaga tatctgtgta ctgccaattg agcgctttac caactaaccc cacgaaagta
     1861 gctagtgcgg gaataattac cagtgcgaac cattttagta cttcgaacgt tttattattc
     1921 attttcttct ctccctaaac aaagttttga tttgttgcgt gtgttccacc aatttttctg
     1981 tatgtgtatc taatctttca tcgtgtttct ttagttcttc atgaatcatc aatcgatctg
     2041 atttgctcga ttctaaatct ttagtcagca aatctaaatt gtggcttact tttgaaagag
     2101 tctcagtaat tttcgagaaa gatgcagcaa tcggtctaat tactaataaa atcaaagaaa
     2161 cgatagcggt tattgatcct gctatcattc cccattcccc taaattaatc atgtgacaac
     2221 tcctttacct taaataaaaa cgcatcaatt aagatgcgct cttatcttta ttaatgattt
     2281 tatctgcttc ttcgtctgta atgcataatg gaacgaaaac cattacttgt tcgttagtga
     2341 aacagcccca gtcatacatt agtttgatat cttcatatga atacatgtta cttcccacct
     2401 tgtaattgtt ttttgatttc ttcgatttcc ttggtatttt gtacagaagc aagcatcgtt
     2461 tttgaattca actgcgctaa actttctgac ttttctttca ataagacatt ttcttgcttg
     2521 attgctacat tgtttagcat tgctttagaa tttagctgtt tcaattcatc attttctgcc
     2581 ttcagcgttt cgtataatgc cttaatatta gccaattcat ctacgctatc aacagcattc
     2641 ccttttttct cttcttcagt agctaactca acccactgtt ttttatcaaa atcaaatctt
     2701 ggtctccagt taggaactgg tggtttaatc tctgtacagt tttcagggat attttcttga
     2761 ttgttcaaaa tgatttgctc gaatccgtaa ggcttgattg atttgtaaac tactttcatt
     2821 agatttcctc ccttaaattg aataagtgat aacaaatgaa taagctgatc cataacttga
     2881 atttcttctc catttaatgg ctccgtctgc gccaatagat agttgagcac tgttcaaagt
     2941 agaacggtct attgacccaa ccagttgttc aaaactaatt ggtgtccgat agccttctgg
     3001 aacagttaaa ataaccgaat cattcccgcc actacttttt ccgtttaaag ccacaaaata
     3061 tatagaaact gtttttcctt cacgataaag ctttgctgtc cctgtattac cgttagtgac
     3121 aaccagattt accacttcat atctgttatc atcagtcgtc aaaacacgtt tattattgat
     3181 tgttacagaa cctgaaaaat tcttatgtcc tgcaatggct tggtctccat ctgtctgcac
     3241 caatgcttct ttaccatcaa tagcttctac atgcgttttt aagtatttag caactccgtc
     3301 ttcttttaat tgaacaatat cagccattaa accgtcccca ctttctcaaa tgtaatattc
     3361 gctaatccat caagtttcac tttatcggtt gctgacatta aacctgctgt cgtagttgta
     3421 gcattacctg gatttttctg tgctccagct gcaattccat ccaacttagt tttatctgtg
     3481 gacgacatca acccatttgc tgtagttgta gctacagctg tagttgtggc atttattcca
     3541 ggatcacctt tatctccctt tggtaaaaca aaattaaatc tagctgcaga tgatgttcct
     3601 acattcgtaa cagaagcggt tgaaccacta gaaacggttc ctatggtaat tgttgctgct
     3661 tggccaggat cgcctttatc tcccttcacc attgttggtt tgccttctat agcattccaa
     3721 tgagtttgtg gataaacctg tacattgctt tgttttattt ttacgatatc tgtcattctc
     3781 tatacctccc caattttttc aaaagtaaaa tttggaattc tttcgtttgt gtaattttct
     3841 gcttgattta cagcttcttg gaatttttga tctacatatg actgattaac accaccagtc
     3901 ccactaccac ctgtagaact aattgttcca tcttctgcaa ttgatatatt tgcacctgct
     3961 tttaatattt ttagagattc taattttcct tttaattctt cagagaaatt gaagtctgtt
     4021 tgcttagttg cagataaaac accttcttca gtaacttcta agagttcccc aacttttata
     4081 cctcctaatt gttcaggagt agcaatcggc aaaatatatg ttccaccttc tccatttacg
     4141 atcttttgaa acatttcagc agtaagaata ccatcgcttg tctcgcttgc ataaggtagt
     4201 tcagtaattg cattctctaa tcctaaatca gctttcgtta aaatgactgc accatattta
     4261 ccattaaccg aaagaacttt tgattgtcct gatatcattt tttctaagcc taaaactgca
     4321 gagacatgtg taattggcat aaattgacgt ttaacaccag attcatcagt ttccatcatt
     4381 ctctttactt taaccatcaa atcaccccaa ctttctcaag agtgaaaaca ttctgttttg
     4441 gatcatcaac agttgcaata atcaaagctc cttcttcaat tggaaattct actgtttcaa
     4501 ttttttcgac ttcgtgatta tctgaaaaga gatcgtcttg aagaatatca gtcacttcaa
     4561 tttcaccgta ttcaatcgtg aaaagtgctg ctttcatttt ttgatacaaa taatttagat
     4621 cggataggaa acgttcagaa atcgattcat ggcgtacacc ttgaagatct acacgtgcat
     4681 ccattaattc ggctaacatt actccacctg gatcgataga cttcaaaata tccttgattg
     4741 attcaaacca tgacaagtaa tctgattctt gtccttctct ccaatcttgg aaactattct
     4801 cctgttcctc tctccagcga tcaaattctt ctttcctttc attcatccag tccgtaaaat
     4861 cgcctttatt ttcattaata aaatctgtca tatcagcaat taaatcttca attgattgcc
     4921 aataagaacc catttctcct tctgtttttg aaacagcatt gatgacaaaa taagagaagt
     4981 cttgagttgt tccaatcaag tcttctcctt taaatataat gaaattggct gtttgtcgat
     5041 gcaaacactg catggaatat ttatcaaaaa tatatttgat tttccctttt ttagcatcca
     5101 caatttttgt ttctaattgg actggatatt ttccgccaac aactgattca aaatatacct
     5161 tacattctga taaatcatat ggaagaccat tttcgacaat tgtagcttcc ataacttcag
     5221 tgtttttatt gccttgtcga acttgaatca tccccacgta attatagggt tctgttgtac
     5281 ttaatatgac attccacttt gccattaaat cacctcccta ttttggtggt atgacaatgg
     5341 aagaaattgc acttgcacta taatactgtc gatctaactt tccacaaatc atacctaact
     5401 ctgtgttttg ttcataagtt tgcatacgac cattagctaa gcctcggata acgccagtat
     5461 gtccataagt tcc
//
LOCUS       DACDHL010000133         5372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDHL010000133 DACDHL010000000
VERSION     DACDHL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.136"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            396..1355
                     /locus_tag="IV228_002656"
     CDS             396..1355
                     /locus_tag="IV228_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8718498.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(1442..2452)
                     /locus_tag="IV228_002657"
     CDS             complement(1442..2452)
                     /locus_tag="IV228_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8718499.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNGGKQFRVYTDGRISQRIGMTNNFQM
                     IK"
     gene            complement(2534..3424)
                     /locus_tag="IV228_002658"
     CDS             complement(2534..3424)
                     /locus_tag="IV228_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HAP8718500.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            complement(3417..3713)
                     /locus_tag="IV228_002659"
     CDS             complement(3417..3713)
                     /locus_tag="IV228_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718501.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            complement(3869..4414)
                     /locus_tag="IV228_002660"
     CDS             complement(3869..4414)
                     /locus_tag="IV228_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8718502.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            complement(4588..4887)
                     /locus_tag="IV228_002661"
     CDS             complement(4588..4887)
                     /locus_tag="IV228_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8718503.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            complement(4914..5222)
                     /locus_tag="IV228_002662"
     CDS             complement(4914..5222)
                     /locus_tag="IV228_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8718504.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
BASE COUNT         1640 a         1002 c          818 g         1912 t
ORIGIN      
        1 ccttttttct cacttttttt caaaaatatg gtatgcttct tagtaaattc aaatatatgg
       61 tatgcttctt agtgaattca aatataaaag agtttaaagc gtaatacact tatggggaag
      121 tggtttgggg tgcgctttaa actcttcttt attattatct cacagcatgc ttaaaaggtc
      181 tatggattaa gttatgtaaa aaagtgcctc tttaaacgtc ctaaaatcca aacaaggata
      241 gcataatgta cactatcgtt gatccagaaa acaggtaaat aaattttgca aaagcaataa
      301 gtcgccgatt gtaaaattaa gctagacaac taaaaaagtc atttgtgcta cactcaaaat
      361 agttccgacc aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaacgg
      421 atgaacttgt aataatagag tcttatttca aaatgaatca atctgttgct aaaactgccc
      481 attgcttgaa tcgttcaaga caaacgatcc ataaagtata tctgttcttc aagcaaggaa
      541 aatcagcctt agaatattat caacagtata agaaaaacaa atcaaactgt ggtagacgtc
      601 cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt caaggatgga
      661 cacccgatgt gattgttggt cgtgcagcgt ttcctattag ttgttctgct cgtaccattt
      721 atcgtatgtt caaaaagggg ctatttgatt cttctgactt accgatgaaa ggcaagcgta
      781 aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct attcatgaac
      841 gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt gacactatcg
      901 taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca aaagttatca
      961 tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta aatcaatggt
     1021 ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga aaggaatttt
     1081 caaattggaa acagatcagt aatgtcaatg atattgccat ttatttcgct gatccaggaa
     1141 cgccgtctca aagaggccta aacgagaatt ctaacggatt gttacgtaga gatggtttat
     1201 tgaaatctat ggatttcaat tcagtagatg aattttttat tcaatctgtc gcatctaaac
     1261 gaaataatat tcctagaaaa tcactggatt atcgaacacc tttggaagta tttttgagtt
     1321 acgtaagtat tgatgatctg tctaacttaa tttgacaatt aagattataa aaaaactgat
     1381 tctaagaaaa ttaaataatt tttctcaaaa tcagtttttc tttgataaat aatattttta
     1441 attatttaat catttgaaaa ttgttagtca ttccaatacg ttgagaaatt cttccatctg
     1501 tataaacacg gaattgtttt cctccattca ttaccgtatt tccaatatca tcagtagaaa
     1561 tatcaactgg ataaaattct ccattttcat cttgcattaa atcttttcta tattgggtgg
     1621 attcaataat attagtattt gcttcatttt tatctggaga ctcataaatc cataacgctt
     1681 tattatttag tacagtttct ttagcagctt cccaatttga aattcctgaa ctacttgtag
     1741 attgtgtcgt tgtagttgtt gcttgttgag tagattcttc tactactggt gtttggggag
     1801 tttgtccatt aatgatatct gtaaatcctt gttttagctg ttgattttga gtttcgttaa
     1861 agtacagata attattttca ttaccttgat tttgttgcgt taaataaact ttaggttgtc
     1921 cattttgaaa accgaaaaag taaacatgct gccctaaata ttctccagtt tctacatcag
     1981 aataaactgc tactaaatta aatcctgcat ttccagttcc atcttctgac cattgtacag
     2041 taactggtgc ttcattgatt gccattttcc aatttccatc aataactgca cttggtaaag
     2101 gcgtaccata cagacttaca ttgttctgag gattgtagct tttatattct tgacctaatg
     2161 tctttcccca ttgtgggaca aaagttgcta attgagatgc tttatttgta tcccataatt
     2221 cttgttttat ttgaaaatca ctcgtatctg aactcgttgc cttactagaa gactctgttg
     2281 ttttagtttc ttgagtagtt gtttctattt cttttgtaga agattccttt cttgttgttt
     2341 cagtttcata agttgttgaa tccacttgtt tcttttgttc attttgagag acagtagaac
     2401 atcctccaag taaaagaatc gtgcctaata ggaataatcg ttttgctttc atcctttttc
     2461 ttctcctaat aataaatttg aattcattca agaaaaagaa ccacccgtat aagtggttgc
     2521 tttttctaaa taattatttc tttaacagct cttgcttcaa ttgttgaaac tcttcttctg
     2581 taatcatatt cgtttccaac aagtgagaaa gtctttctaa ttgagaaaga actgtatcag
     2641 aagacacgat atcagaagat acttcttcaa attcaccttc taaaatatct aaatcttgtt
     2701 ggagattttt ttctgtatac gctacatttt tccctaactc tgtttgaagc ttcgttgcca
     2761 ttggtttcat agacaagtaa ttgatgcctc ctgaaacaac gcctcccaca actggtactg
     2821 ctttagaaat acctttcgca aaggtatttt tagttaactt tgtaccaaaa atacctagta
     2881 ctcgtttgat tactggataa tacagcgttt gtgtcaatgc tttttgagga atctttttca
     2941 atgcttgtgt agacagcttt ccagacatcg ctcgtacggc agaacctgca gaagttactc
     3001 caagcataat tcccaaatac aatatcagcg tattctttgc ttcttcactg aactctccag
     3061 tctctcccat taaatcctta aatccataaa tatacgaaat ttcctgtgct aatcgtaatg
     3121 aataaccaaa gaactgaagg agatccgctg gaatagttgc tgccatagcg atacctcctg
     3181 gcagtccagt tgctaacgat accgcacttg atttcaaggt aatcgattga atacattttt
     3241 gtgcttcacg atctagcatt tctttggata aaaatgcttg tggacccttt tctatgagtc
     3301 cttcaatatc agaaacattc ccttgaaatg tttgaatcaa aaatgctcga cgatcaactt
     3361 tgaccattgg taatttgaat gcttcttcta tgattctcaa acctactgtt tcttcattag
     3421 ccatgtacat gttgctcctt ttgttgttgt ctgataatac tttgaatcca tctcgttgaa
     3481 aaaccatacc gatccgttaa tttcctagaa tcaatggaat ggttttcttg tatcattgtt
     3541 tcaatttttt gagcgactaa ttctcggtca tacattttca ttggaaacga caactgttgc
     3601 ccacgaaaag cgatataaaa ctgataaagt aaatccgtgt ctagtccttt ttcctcaaaa
     3661 aattcataga tacttttata cgaactcgtc aaacattccc catctacttt cacttgctca
     3721 cctccatttc ccctgacatt ttaaaaatat catattgatt ccttcattga taatgcatat
     3781 aacaagaggt tttacaagtt gtttagtctg caaaagaaca agtaaaataa cagaaaaaga
     3841 aacatataca ttgaatactt attttcgttt attgaaataa caaagaatga tccgacaatt
     3901 gccattcatt acgaacatga atacttaatg ggtgagataa cttgccttct gcatttatca
     3961 atactttggt catcttcttt tgtttcgcac tcaattgatc gtaaatggtc tttgcttgtt
     4021 cataagccac ttgactcgtt gccgtaatcg aaccacaagc aaaaataata gtgttgattt
     4081 tcttttctgt aaaaatggtc gttaatattt ccaaagattg ttcatcattg ccacgagaaa
     4141 atacccgagg acttttcgct tttgtatacg aaaaaagatt cacggcaata aaaccagtaa
     4201 atcccaattg tcggatattc ttttcgatta ataacatact caaatcattc gtaaatggtt
     4261 ctgtatctgt tggacgaatg gtaatgacta ctgctaattt ttctttctct tcttttcctt
     4321 tgattgccca ttgtcgtttg aaaacatagc gatgggtatg atgaggatct cgccaaactt
     4381 ctgtttgtgt ggtcactaat tctttttcaa tcatctcttt ttctcctatg ctaatttttg
     4441 ttcaagcaat tcaattacca ttgccgttat aagtgtcaat actagttgaa aatatttcat
     4501 attctttctc cttttttctt cagaattcga taaatcgcgg taaggatatc ttctaaacat
     4561 ttcgcaatca ttctcaaata atcgatctta ttcattcgtt cctccaatct gactccaaac
     4621 caatcgtcct tctaccagta acccacaaac aaattctcgc tggctggtaa tatctgtaat
     4681 gaaataaact acatagtctt ttccatcttc tggtgcgcaa taaatataaa gatgtgtcat
     4741 cactaaacgt ggtagatgaa catttttagg aagcgtaccc atttcaattt cctctttttc
     4801 catcgtaacg gattcatagc cccactcttc aggagtagtg actagctgtg cacacttttc
     4861 cagtacttcg atagccgttt ctaacaagtc atttcgctcc tttcttttag atatcagtat
     4921 tcttctggta ataacatcgt ttgatactca cccgaatcaa tgacccatat tttttgagac
     4981 actgattcca aacgaagctt aggtgaaatc agatactctt ttcttgcttc cggttcttct
     5041 tgacgatgaa ccaacttttg atttcctttt tctatcgaaa attcaaagat ttgaagatag
     5101 tctaaaggaa gcttcttttg aacgttttga tcgattaaat tccaacataa agtttgtaat
     5161 tccaccagta aactgtccat tactcccttt gtgacataac gttgtccttt attttgaaac
     5221 atttcttttc cctcctttgt cgtaaaaaat agcccaatct tctataaaga agattggact
     5281 tcatggatat aatatatatt tgaatttatc tctatttcat tgctatatta tttatttcaa
     5341 ttaactgaat agacatcaca actaaatata ag
//
LOCUS       DACDHL010000134         5311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDHL010000134 DACDHL010000000
VERSION     DACDHL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.137"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="IV228_002663"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="IV228_002663"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8718505.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="IV228_002664"
     CDS             complement(1297..2133)
                     /locus_tag="IV228_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8718506.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="IV228_002665"
     CDS             complement(2317..3534)
                     /locus_tag="IV228_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8718507.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3917..4000
                     /locus_tag="IV228_002666"
     tRNA            3917..4000
                     /locus_tag="IV228_002666"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5237)
                     /locus_tag="IV228_002667"
                     /pseudo
     CDS             complement(4240..5237)
                     /locus_tag="IV228_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DACDHL010000135         5207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDHL010000135 DACDHL010000000
VERSION     DACDHL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.138"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..1367
                     /locus_tag="IV228_002668"
     CDS             33..1367
                     /locus_tag="IV228_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718508.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1354..2988
                     /locus_tag="IV228_002669"
     CDS             1354..2988
                     /locus_tag="IV228_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718509.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3163..3996
                     /gene="msrA"
                     /locus_tag="IV228_002670"
     CDS             3163..3996
                     /gene="msrA"
                     /locus_tag="IV228_002670"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8718510.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4076..5065
                     /locus_tag="IV228_002671"
     CDS             4076..5065
                     /locus_tag="IV228_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8718511.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttag ttgtctctat
     1741 attttctctg cttatcggac agtagacatc tactatcgtt ccgcaattgc cgaagctatc
     1801 gcaataacac ttgttatccc tgtattattt tatgcttatc aaatcatctc tggaaaagaa
     1861 gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt tgctagtgta cagccatgta
     1921 ctatcaacat taatgagtac agcattaatt atcattttta tttttattcg tttggtttct
     1981 aaaggtttca agaatgcaga ttttattgct atatttaaaa aattatttag tgcggcagga
     2041 atgacattgg ttctgacgag tgctttttgg tatccgatgt ttgaacaaat gctctaccaa
     2101 aaaatcaaca aacctagcgt gacaaattta tatgctcatg catctaatgt gttcgatagt
     2161 ctgactgaag ctatgaacaa cgacttaact acttactcaa tgggactagt tggtctgtta
     2221 tctttatgta ttcccctgat tttatttaaa aaattaacaa acatagaaaa aaagatttat
     2281 tatggtacgt gtttgacttg gctagctaca actagtttgg tgccatggta tcttctgcaa
     2341 aatacgccag ccaaattgtt acaatttcct tggcggatct taagcttgca aataatattc
     2401 tcttctctga tattaacgat gatatttttc aaaaatagaa gatacaataa gacaagagaa
     2461 ctattttatt tatttatgac tgttttgtta ttaatcactt taactgtttc tagtaaaaaa
     2521 aatttcaatc aaaaaataat ttctcagccg ggacatattg ttgtaaacaa agaaacagta
     2581 gaggcgtata ccactaaagg aatggattat tttttatatg attattctcc tatcgcttca
     2641 ttggagaata aggagcatat aataaaacac gaagcaaaga ttggcagtca atgggtaaac
     2701 cttccttata aaacaactgg tagttctatc atatatagag taactaatga gaagagccaa
     2761 gtaatcacac ttcctgtata tgcttattta ggaacgaatg tagaaataaa tcatcaacct
     2821 gtaaaggagc aggtgaatgc taatggattg gtacaaataa gcggagtcaa aggagttgct
     2881 gatattaaag taagcacaag ttatacagta acgactatta tttcttatct tgttacatta
     2941 actgcatatt tatgtttatt ttttaaaata gttttccata aaaggtaaat taacttttca
     3001 tttataaaat aaattgatta aaaaggagaa tctgtcgata aggattctct cttttttata
     3061 cgaaaaatct gtagggggat gaagtaatcg cttatttcct cactttatga taaattacag
     3121 ctaaagtcac tagcttgtct tcttcggaaa ggaggagaat ctatggatga ggaacaaata
     3181 ttcattcaaa cactttataa tctgattttg aatccgaata cacgagattg ggaacgaaaa
     3241 gtattgattc agacaaagaa tgacataaga gaaaatatat ccgttaaaga acagttatca
     3301 aaactagaag caacactacg tccattagct atccgaatga atctgacgcc tgatgtaatg
     3361 gacttttatt tattgattac ggaaggattt gataaggagc aaaaatatga cttttccaag
     3421 catgccatgc aagatgctga ctatcaagaa cgtgcagtat ttgcaggggg atgtttctgg
     3481 tgtatggtcg agccgtttga atcgaaaaaa gggatcctat ctgttttgtc aggatacacg
     3541 ggcggacatg ttgaaaaacc aaattatgat caagtaagtg gtggttacac aggtcatgtg
     3601 gaagctgtgg agatcattta tgacacaaga gaaataagtt atagcgagtt gctgacaatc
     3661 tattggcaaa ttactgatcc cacagataca tttggacaat ttcaagatag agggaagcaa
     3721 tatcgtccag tgatttttta tcaaggcgaa cgacaaaaag aattagcgga acaatcaaaa
     3781 cagaaactag acagttcagg aactttccac caaccaatcg tcacaaaaat cgaaccagcc
     3841 ggtacgtttt ggccagcaga aaactatcat cagcaatttt acaaaaaaca accgaaacgt
     3901 tataaaaaaa tccagcaagc acgtaatcaa tttctgatct atcaacgagt aaaaaacaaa
     3961 tggcaaaaaa atatacgaaa aaatcatttt gattaactcc ttttctttaa aatgtagtaa
     4021 actaaaaata gaaaaaagaa agcgttttaa aaagatttga tgaggaggag aaaaattgta
     4081 tattgcagat agtgcacgtt atgaaaaaat ggaatatcgt aaatccggtc attcaggttt
     4141 gaaattaccg attttatctc tcggattatg gcaaaatttt ggtgattatg atcctatcca
     4201 taatcaaaga gagattttaa gaggcgcatt cgacatggga attacccatt tcgaccttgc
     4261 gaataattat ggaggccctg ctggagctgc agagaaaaac tttgggcgta tttttagaga
     4321 agattttcaa gcctaccgtg atgaactgat tatttctagc aaagcaggtt atcatatgtg
     4381 ggaagggcca tatggggaat ggggctctcg taaatccatc atttcaagtt gtgaccaaag
     4441 tctccaaaga atggggctag attatgttga catcttctat catcatcgcc cagaccctga
     4501 tacacctttg gaagaaacag cagaagcttt gatgcaactt gtaagacaag gaaaagcatt
     4561 atatattggt atatccaact ataatggaga agatacaaaa aaaatgactg aaattttaaa
     4621 aagaaaggaa gcaccgttca ttattcatca gatgcgctac aatatgtttt ctcgtgcact
     4681 actagaagac gatctatctc cagtattaga agaagaaggg ctaggggcaa tcacgttcag
     4741 tccgcttgct caagggttgc tgacaaatcg ttatctacat ggtattcctg aagattctag
     4801 agcacatcgt aaggaaatac cattcctttc agaagaacaa gttggatcaa ctctggaaaa
     4861 aatcaaagca ctgcaaacaa tcgccgtatc acgaggacaa tcattagcgc aaatggccct
     4921 tgcatggaat ttgcgacaaa aatctgttac aagtgtactg gtcggagcaa gtcggttaag
     4981 tcagttacaa gaaagtgttc gaatgatgga taatttagat ttctcaccag aagaagaatt
     5041 gagaatcgac caaattctag aatagaaacg atgtaatcaa actttttatc catatattaa
     5101 aaaaaacaaa agatgacatc aagaatcgaa tgaagatcgt ctgttattga acatcatatt
     5161 ttttgtcgtt caaaataaaa atgatcgaag gctaggagtg aatttta
//
LOCUS       DACDHL010000136         5126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDHL010000136 DACDHL010000000
VERSION     DACDHL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.139"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            225..824
                     /locus_tag="IV228_002672"
     CDS             225..824
                     /locus_tag="IV228_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8718512.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            873..1589
                     /locus_tag="IV228_002673"
     CDS             873..1589
                     /locus_tag="IV228_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAP8718513.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1603..2382
                     /locus_tag="IV228_002674"
     CDS             1603..2382
                     /locus_tag="IV228_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP8718514.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            2465..2821
                     /locus_tag="IV228_002675"
     CDS             2465..2821
                     /locus_tag="IV228_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8718515.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            2811..3077
                     /locus_tag="IV228_002676"
     CDS             2811..3077
                     /locus_tag="IV228_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8718516.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            3236..3514
                     /locus_tag="IV228_002677"
     CDS             3236..3514
                     /locus_tag="IV228_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718517.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(3511..4192)
                     /locus_tag="IV228_002678"
     CDS             complement(join(3511..4158,4160..4192))
                     /locus_tag="IV228_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8718518.1"
                     /translation="MNHFKGKQFKKDVIIVAVSYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLDYLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFSVCTEI
                     RILLGIPA"
     gene            complement(4224..4385)
                     /locus_tag="IV228_002679"
     CDS             complement(4224..4385)
                     /locus_tag="IV228_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718519.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(4709..5041)
                     /locus_tag="IV228_002680"
     CDS             complement(4709..5041)
                     /locus_tag="IV228_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718520.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
BASE COUNT         1772 a          836 c          930 g         1588 t
ORIGIN      
        1 ttaaaggggc aatattaatg ttctgagata atcaaatctt accgaaaata tcaaaagtcc
       61 aatgtgacac aattggattt tgtcacattc gttaaaatcg ctccatcctt agaggctcta
      121 ataggaaatc acttttcttt tagcgccttt tttctatagc ttgtttaaat gtgacttatt
      181 atctgttata atcgtattaa gtaacatttg agaggtgtct aaaaatgaaa agcgtggaac
      241 cgattcgcga caaaaaaaag atcgatgcca tgaaggccat tttggcttcc ggaaaatatg
      301 ggcaacgaaa tttggtgctt ttttcgatcg ggatcaatac agcctatcga atctccgatt
      361 taagacagtt aaaattaagg gatgtgctag aaatttcccg tgggcgggtg atcgtcaaag
      421 aacgtctggc catgaaggaa caaaaaacgg ccaaacacaa ttcggtcttc atttcgaaca
      481 aattgcgaaa agtgattctt gattatgttc aatccgaatt tcctgagcag ttgcaagcac
      541 aagacttcag caagtatttg tttccaagtc gaaaaggagc agatacgcct ttgacccgac
      601 aaagcctttg gcggatcatt catgaagcag gaactgcagt tgggttaaaa gaaattggtc
      661 cccattcgat gcgtaagacc tttggctatt ttttatacaa gcaaggaacc aaaacagaga
      721 tcattcagtc gctgctaaac cattcttcac aacgggagac cttacgctat attggaatca
      781 cccaggaaga caaagatact gcagtaaaga gtttggattt atgacagaat aatgccctat
      841 ataatagaag gaaaaattca agaggtgaga aaatgaacga agaaaagcag cctaactttc
      901 ctgataaata tcacttatct cgcaaagagt ctgtgtattt attgaagaaa aacattgtag
      961 aactggtgta taatgcgggg aaatttgaag ggttaaatac aacgttgtta caaacagaag
     1021 ggattattaa gtataatcgg gccaacaacg tggctgtaga tgatgtcctg accgtcgtta
     1081 acttaaaaag aggctttgaa atgcttttaa atgacgtaca ggagcccctg ttagagacaa
     1141 gtaagcggat taatcggatc gttgcagcag aggatgcgct ttttcccggt gaaatacgaa
     1201 ccggtggggt agaattatcg acgattcaag gaagatatgt gccaccaatg ttaactgaag
     1261 acgaggtaaa caatcaatat ggtgagataa tgaatcaaga gatatcagat acggaaaaag
     1321 ccctgcgact gtttctattc atttcgaaaa atcaaatatt ctggaatggt aatgtgggta
     1381 cagccttgtt gattgcgaat aagattatgt ttagcaacgg tttaggacta ttatcaatac
     1441 ccgaaaatgt atttatgaaa tttaacgaac ttttatcaaa gtattgcaac agtaatctat
     1501 tagtcaatga gtcaaatatt atatcatttt tatatgaaca gtgtatcttt ggtattaatt
     1561 atttgaaaat aatccaacat acaatttaag aggtataagt aaatgatgac tatagacgaa
     1621 gcaaaacaat tagcaactta tttttcagtt ggttcagcgg gatatatttt agactttact
     1681 aatgtaacgt ggaacatatt tacaaagaaa atcgtaggga ttgaaataca aaatcattat
     1741 gggggctcaa agggagtatc ctttcaaaag tttctgcttg acgatcaatt tgatgattcg
     1801 ttaaagaatc ggttaatcca atctttaaaa ggtattatgg atcgagataa aaaatatgaa
     1861 tctactgaaa tgaatcccat agttcagttg ctttttgagg aagtatttaa agaagatact
     1921 tcaactgcat taagaaaaat gaaattttca agcgagattg atttacttta tattcggcag
     1981 ctacccatta ggattcaaac agatattaaa aacaaggatt ttgactctgt tttgacaaaa
     2041 gcaaatactt taatagatga agttctaaaa tttattattg aaaataccga aggaccgagt
     2101 gttaatatta atttatttaa gtcaaaagaa ttaagaaaag aagtatttgg ccgcctcaat
     2161 attaaaactg aaaaagatat ggataataga atcaaagccc ttgttggagc actcaataca
     2221 ttatcagata aaatactcga aatgagaaat agtcaaggag atgctcatgc acatggctca
     2281 aatagaatag taattaagga agaagaagca attcttgttg caaattcttc tatggttttg
     2341 tgcgagtatg tgtttactaa gtataaagga ttaaatagat aataaaatgc atattttata
     2401 gtatacaatt cgaattttta tgttatatac tataatataa aatcaatata tgatataatt
     2461 gggtatggaa aaaatatctt ttgaaacatt taaaagaccc aatggtcatg atgaattttt
     2521 agaatggttg gaaacattac caaaaaaaga tagtgcgaag cttttacgta caatagaaga
     2581 aacagaaaag aatgggatgc tagtagctca acggttgaaa tgggtgaaaa aattagacac
     2641 taatttgtac gaattaagaa gtaaggtagg atcaaacatc caaagagcca tctatttcca
     2701 tgtggacaat ggaaggtatg tcattactca cggatttact aaaaaaacgg agaaaacacc
     2761 aataactgaa ctcaagcatg caaaagaact tagagaggag tggttcacaa atgatgatta
     2821 aaaaattgat tgaagaaaag aaaaaagata aagattttgc agaagcatat gaccgtgaag
     2881 gaagaaaact cgaaactgcg gttgctcttt atcatgctcg ggaggaagca gggctaactc
     2941 aagcggagct tgcacaaaaa gcacatacaa ctcaatctac tattgctcga attgagagag
     3001 gggataacgt atctttcgat aaacttgatg atatcgccag ggcattaggt aaaaagctgg
     3061 tagtagaatt tgcttaaaag cgagttgaaa atatatctat aataggctcc taactaatca
     3121 attagtttag tgggcctttt tttactagat agataaaggt tgtactatag tgtaaaagac
     3181 aataaatttt atactaaaat tactttaaag agaactagag agaaaggtac aataaatgaa
     3241 aaccataaat cttataaaag tatttatagc atcaccaggt gatatagcag caaaaaggaa
     3301 tgaagtagaa gaaaccatat atagatggaa tatagaaaat acagataata caaaagtgat
     3361 tttgatgcct atacgatggg aaactaatgc gattcctact tatgtaaata atagctctgg
     3421 gcaaagtgtt attaataaac aaattgtaga aaatagggtt ctgctgcaaa gttttaaaaa
     3481 taaaatcagt ttttgtatta tttcttcatt ttaagctgga attcccaata atatcctgat
     3541 ttcagtgcag actgaaaagc cgaagagagt gccttctttt cggcttttct tgtagattcc
     3601 tcgaatggtt tccatccctt taattgtggt ggaagctgta cgtagacttt gatacaattt
     3661 atgtcgacgt tttatcgatc gatgatcttg ttctattaaa ttgttaagat acttcacagt
     3721 tcggtgctct gtcttagtat ataaacccac actctgtaac tttctaaagg cggagccaag
     3781 agaaggtgct ttatcggtca caattgcttt cggctcacca aactgtttat ggagtcgttt
     3841 taagaaagca taggctgctt gcgtatcccg tttctttcgt aaccagatat ctaaggttaa
     3901 gccgtccgca tcaattgcac gataaagata atgccaatgt cccttaattt tgatataggt
     3961 ttcgtccatt ttccatgaat agaaggattg tctatttttc ttcttccaaa gataatcgag
     4021 gactttgctg tactcttgca cccaacgata aatcgtagta tgacaaacat ttattccacg
     4081 atcatataac aattcctgaa cttcacgata gcttagattg taacgcaggt agtaactaac
     4141 agcgacaata atgacgtcat tttttgaatt gtttgccttt aaaatgattc attactctat
     4201 cctctctgtc ttttttctca attttacact aaaatagatt ttttggaaaa ctttgcaaca
     4261 gaaccacttt tcgtaatatc aatgtcttta ttcgtatgat ttttcgtttt acgatctaca
     4321 tgattttgaa gtccatttaa cgacccaact ttatgctttt gtgttcgtgc caccatatag
     4381 gccataactg tgtacttcct ttcgttaaaa ttattgcgag aatcacaagc taaattcgga
     4441 aaaacctcca tgtaaggtat aacttactag acttatactg cgtaaagtct gtaagctctg
     4501 ccgagggcta tatcggctta acagccgatg agaaactccg tttctctcaa acgaaaaagc
     4561 gaacgaaaaa cgactgcctt cgcattcctt ttttacgtcc ttttttcgtt caaaaccatt
     4621 ggctttttgc gtcattgttt tcacaatttt tattccgcaa aaaaccaaaa gtgaaaagta
     4681 ttgcttttca cttcaacccc tcctcaattt agttcacatt ttctgaacga aattgtgcca
     4741 taaattgagt caaaccctta acatcattcg tacgccaata tgcaccattt tcttgccaaa
     4801 ctaatccatc tgcaaatttt cttagcgata ctaaaacctc atcatttact gaatgatttc
     4861 ttttatttga ttccaaaaaa tctaatgctt cagcaggtgt cttaaagcct tcctttttac
     4921 aatataattt cgctaaagaa atcatttctt tctgtctaat ctgttttcgt ttcgctctaa
     4981 tcttcgcttc ttttgctttc aaatccgcca tttcttttgc caatatttct ctattgttca
     5041 ttttgattcc tcctaaaaat aaggcatatt tctagtttaa aatatttatt ttacgaatag
     5101 aggatttgaa caacgaatga cacaaa
//
LOCUS       DACDHL010000137         5089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDHL010000137 DACDHL010000000
VERSION     DACDHL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.141"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IV228_002681"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IV228_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8718521.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="IV228_002682"
     CDS             complement(2066..3064)
                     /locus_tag="IV228_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8718522.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="IV228_002683"
     CDS             complement(3290..3943)
                     /locus_tag="IV228_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8718523.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IV228_002684"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IV228_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8718524.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1510 a         1042 c          773 g         1764 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacatatgaa acagcgtata
     4921 aaactgtact attattttat ctcaaaagct aaatgaaacc gatttcttaa gattttttat
     4981 gttttttatt tctttaatag taatgttttc tgtcatttta aataatactg ttctttatta
     5041 taacgcttat tttctctgtt atataagcgt agtattttaa agcaatgat
//
LOCUS       DACDHL010000138         5087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDHL010000138 DACDHL010000000
VERSION     DACDHL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.142"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IV228_002685"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IV228_002685"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP8718525.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IV228_002686"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IV228_002686"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP8718526.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IV228_002687"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IV228_002687"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP8718527.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IV228_002688"
     CDS             complement(4028..5044)
                     /locus_tag="IV228_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8718528.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DACDHL010000139         5008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDHL010000139 DACDHL010000000
VERSION     DACDHL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.143"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            275..1753
                     /locus_tag="IV228_002689"
     CDS             275..1753
                     /locus_tag="IV228_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8718529.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="IV228_002690"
     CDS             2030..4090
                     /locus_tag="IV228_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718530.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="IV228_002691"
     CDS             4173..4742
                     /locus_tag="IV228_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8718531.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1813 a          816 c          922 g         1457 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaa
//
LOCUS       DACDHL010000140         4866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDHL010000140 DACDHL010000000
VERSION     DACDHL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.144"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(92..1702)
                     /locus_tag="IV228_002692"
     CDS             complement(92..1702)
                     /locus_tag="IV228_002692"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8718532.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2895..4235)
                     /gene="glnA"
                     /locus_tag="IV228_002693"
     CDS             complement(2895..4235)
                     /gene="glnA"
                     /locus_tag="IV228_002693"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8718533.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4263..4655)
                     /locus_tag="IV228_002694"
     CDS             complement(4263..4655)
                     /locus_tag="IV228_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8718534.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1437 a         1046 c          797 g         1586 t
ORIGIN      
        1 aaataataac aaatcagaaa aagagaaaag tgacaagaaa attgcttatc cccttctctt
       61 gctacttttc tctttctatc aagttcaaaa attattttgt ttcgtgatct gctaatgatg
      121 ctccgacaaa tgctttgatt aatttttgcg ggcgatttgg acgagaaatc aattctgggt
      181 ggaattggca tcccacaaag aattttttct ctggcagctc tacgatttct actaaacggt
      241 tatctggtga gacacctgaa aagactagcc cttgttcttc aaacaattga cgatatttgt
      301 tattgaactc atagcggtga cggtgacgtt cttgcacaac atcggctcca tcatatgctt
      361 cagccgtctt cgttcctttt ttgattttac atgggtataa tccaaggcgt aaagttccac
      421 ccaaattctc gatgttttcc tgatcagcca ttaaatcaat aatattgttt gctgtatctg
      481 gattcgtttc agctgatgac gcatcttcta atccaacgac gttacgtgca aattcgacac
      541 aagccatctg cataccaaga caaataccta aaaacggaac gtcattttcg cgagcataac
      601 gaatcgcttc gattttccct tctactccgc gatcaccaaa tccaccagga accaagattc
      661 catctgcgtc tttcaaacgt tcttctacat tttctctcgt taattcttgt gctttgatcc
      721 aatcgatctc gatgtctgaa ttataggcga aaccagaatg tttcaatgct tcaactactg
      781 agatatatgc atctggtaac tcgacatatt taccgacaag cgcaatccgt acttttttct
      841 tcaagttcaa gacacgttct tctaattctt tccattctgt catgtctgct tctggtgtat
      901 ctagttttaa atggtcgcaa acaatttgat ccattccttg tgcttgtaaa actaatggga
      961 tggagtaaag tgtatctaca tctggcgact cgataactgc ttctggttcg acatcacaaa
     1021 attgtgccaa tttgtttttc acgctttgag aaacaggttg ctctgtacgt accaccaaaa
     1081 tattcggttg gatacctaaa ccacgtagct cttttacact gtgctgcgtt ggtttagttt
     1141 tcatttctcc agcagctttc aagtaaggaa tcaatgttgt atggatatac ataacattat
     1201 ctgtaccgac atctgctttc atttgacgaa gtgcttcaag gaatggcaaa gattctatat
     1261 cccctactgt tccccctact tctgtaatga tcacatctga atcagtcatt ttagctgctc
     1321 gcatgatttt ttctttgatc tcatttgtaa tatgaggaat aacttgaaca gttgctccca
     1381 agtattcgcc cttacgttct ttacgcaaaa cttcagaata gatttttcct gtcgtcacgt
     1441 tggagtattt gttcaaattg atgtcaataa aacgttcgta gtggcccaga tccaaatctg
     1501 tttcagcacc atcatctgtt acgaatactt ctccgtgctg atatgggctc atcgtccccg
     1561 gatccacgtt gatgtatgga tcaaattttt ggatagttac ttttaagccg cggtttttca
     1621 ataagcgtcc taaagaagct gcaacaatcc cttttccgat tgatgaaacg acaccgccgg
     1681 tcacaaaaat atattttgtc ataagttcaa aaactccttt ttaatttatg tgcaggaaaa
     1741 gtctcagaca ataaacaaag ctccctattc ttcaataaga atagggagcc gaaatttctg
     1801 taaacttctg accctctaaa agggtgccca aggtgtataa tacaagctga ctaagggaaa
     1861 gtcaagtact caaatgaata ctatccgctt acaatttttt ctttttcgat atcctttgag
     1921 aaattatttc ttaagtcctt tatgagtgca aaaaaaattt aacccacata ataagcttga
     1981 tataaacata gctaacgtta aacctaagca aatcagaaaa tcgttactga aaaattttaa
     2041 gtgaaataat agataaaaaa ataaaacaaa cattatgata gataatatcc cacctaacaa
     2101 agaactactt tttcctactt gcagcaacag tttatatagc ttatcgtaga aacaatcttt
     2161 ttctttcacc cttatttacc tcctttacgt aaaaaacgta gtccacagtt ctcggatttt
     2221 tggcatatct tttctattaa ataatatcaa aaacaaaaca attccactaa ttttttcaaa
     2281 gatgaccttt ttttcaacaa aagtgccttg tagggattca ttgaaggtaa cgaatcacat
     2341 aagtaaaaaa taaggagatg cgacaaaagt cgtgcctcct tatttctaaa taaacggtgt
     2401 tctcctgcca actcctcggt cacaagtcac aatatccatc aaacatgaga agctgttttt
     2461 gaaattattt cttgtgattc ggagttaaat ggtaggctca caacctctgg ataaacggta
     2521 ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt
     2581 cacaaaattt ttcttaatac tcgaaactaa acgtcttttt caaaacctct tggtaaacgg
     2641 tgttcaaaag cttgcatctg cggtaataag ttcagccaat tcaaaaacaa gaaaagctgt
     2701 ttttgcttgg ctattcttat tacttgttgc agggcagctt tttcacaacc tctccactaa
     2761 acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact
     2821 atttttccgt tagtttctct aatactcagg tcaaaacgct gatttcacag cctctaattc
     2881 acggtattcc tgttttaata aagttctaga tattgttctc tttcccactc ggaaacagtt
     2941 tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa aatttgagaa gatatgttgt
     3001 cctaatgcgt caatcattac attatcctta cgcaattctt tgatagcatt atggatagtt
     3061 gaaggtaagt cctggatatg tgcagcttct ctttcttcct catccattac ataaatgtta
     3121 cggtcaacag ctgctggcgg tgtgatctga ttacggatac cgtctagtcc tgcttgcaac
     3181 aatactgcca ttgccaagta tgggtttgca gatggatcaa ctgaccgcaa ttccaaacgt
     3241 gtggataaac cgcgagattc tggtacacgt actaatggcg aacgattacg gccactccat
     3301 gcgacataaa caggcgcttc ataacctgga acgagacgtt tataagaatt gactgttgga
     3361 ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac cacctaagaa atgataagct
     3421 gtttcactta gttccaattc accgtcttta tcatagaaaa catttccttc atcattgaac
     3481 aaagacatat tgcaatgcat accagaacca ttgatcccaa ataacggttt cggcatgaaa
     3541 gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa cgagcttgaa ggtttggata
     3601 ttatcacaag catctaccac atctgcatat ttaaagtcaa tttcatgctg tcctggcgct
     3661 acttcatgat gtgaagcttc cacttcaaag cctagacttt ctaattccaa cacgatgtca
     3721 cggcggcaat tttcacctaa atctgttggt gcaaaatcaa aataaccacc tttatcattc
     3781 aaggtcgttg tgatcccacc gtcttcgtct aatttaaata agaagaattc tggttcaggt
     3841 cctaaattga aagaggtaaa accaaggtcg cgcatatctt tcaatgctcg tttcaaattc
     3901 ccgcgaggat cgccggcaaa tggtgtccca tccggattat agatatcaca aattaaacga
     3961 gcaacttttc catgagcact ttcccacggg aagatcatcc aagtggagag atctggatac
     4021 aaatacatat cgctttcttc aattcgaaca aatccttcaa tcgaagaacc gtcaaacatc
     4081 attttatttt ctaatacttt gtcgatctgg ctcactggaa cttctacatt tttgatcgta
     4141 cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt tttcttcctc aatgattcgt
     4201 ttgatttctt cacctgttat ctgtttcttt accatggtat ccctcttcct tttttaattt
     4261 attcagtcaa agaattgtct ttgcaatcct tgcgactgat aatgacctgt tttgcttaac
     4321 ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa cgtcatgatc tgtcaacggc
     4381 ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt cgtatacacg tttgatccca
     4441 gccatattca atccctctga aagatagtct ttaatctcaa gcaacatatc aatatcattc
     4501 agcgaataca tacgtcggtt tccttcgctt cgttccggat gaactaaatc ttgttcttca
     4561 taatagcgaa tttgccgagc agacaaatca gtcagcttca taactgtacc aattggaaat
     4621 acggacatag atcggcgcaa ttccttttct cccatcagac gtcccctttc gttttataag
     4681 ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg catatgttag gttttctaac
     4741 atgagatctc tattttttat aaaataacaa aaaaataaga acacaaaaaa ggtatctagt
     4801 tgattttcgt accagatacc ttttattttt tattatactg tactatcctt tattagtgtt
     4861 tttatt
//
LOCUS       DACDHL010000141         4849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDHL010000141 DACDHL010000000
VERSION     DACDHL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.145"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(102..2138)
                     /gene="recG"
                     /locus_tag="IV228_002695"
     CDS             complement(102..2138)
                     /gene="recG"
                     /locus_tag="IV228_002695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8718535.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(2255..3937)
                     /locus_tag="IV228_002696"
     CDS             complement(2255..3937)
                     /locus_tag="IV228_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8718536.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(3989..4351)
                     /locus_tag="IV228_002697"
     CDS             complement(3989..4351)
                     /locus_tag="IV228_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8718537.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            4621..4809
                     /locus_tag="IV228_002698"
     CDS             4621..4809
                     /locus_tag="IV228_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8718538.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         1292 a         1115 c          858 g         1584 t
ORIGIN      
        1 acaattgctt gaggtgcgtg atctccgccc attgcatcta cggcaatttt cattgtttcc
       61 actccttttt ccgtcatcag tataaagtat agcacagtct gctaatcgaa ataacgatta
      121 tcctgatttt ctatttggag gctttgcgct aaaggctgat actcaggaag catctgccaa
      181 tcctttatct gccaaagggc tgcagcctct tctctggcta cctccaaaac atttccatcc
      241 gtcaccaagt cagcaatcgc aaattgaggc agaccagatt gacggaagcc aaatacttca
      301 ccaggaccac gtaattccag atctttttcg cttagtacaa aaccattggt ggtctctgtc
      361 atgattttca tccgttccac acctagttca tttttaggat tagcaactaa gatacagtag
      421 gaagcatcac ttccgcgtcc aacacgtcca cgaagttgat gcagctgtgc caatccaaat
      481 cgatccgcat ccatgatcag catgacggtc gcgttcggca cgtttacccc tacttcaatt
      541 acagtagtcg aaactaatat ctgcatctgg ttgtctttga aggcttccat gattacttct
      601 ttttcctgat tcttcatttt accatgtaac aagccaactt catattttgg tgcaaatagt
      661 tcacgaagtt tttcatagat ttccactgca ttttttacat ctaatgcttc tgattcttca
      721 atcagcggac agatgacata catttgatgt cctcttgcca gttctttttt cgtccaatcc
      781 agtacactgt ccaattgagg tgttcttacc cagcgtgttt cgatcgggat acgtccagct
      841 ggaagttcat caatgataga tacatccatc tcaccatatg ctgtaatagc taatgttcta
      901 ggaatcggag tcgctgtcat aaataagaca tctggtcgcc agcctttttc acgaagtact
      961 ttccgctggt tcacgccaaa acggtgctgc tcatccgtaa taactagacc taaattctga
     1021 aattcgactc catcttggat caatgcatgt gtgccgatca cgatgttaat ttccccttgc
     1081 tctagttttt caaggagctc acgacgttct tttgctttcg ttgaccccgt aagcaatgcg
     1141 atggttactt cattgggatc aaacagctgt tgcaagcttt ccatatgttg ttgtgccaag
     1201 atttctgtag gcaccatcaa cgcaccttgg aaacctgctg taacggtagc gtataacgca
     1261 atcgcggcaa ccactgtttt tccgctacct acatctccct gcaaaagtcg ctgcatatgc
     1321 tgcggactgc gaagatcccg acagatttca ttcgttacac gtttttgtgc atttgtcagt
     1381 tcaaaaggta gcttttgagt aaattctttc aatttttgga catcataaag gatctccaaa
     1441 ccattcgttt ctgctttttc tgcttttttc aatccttgaa gacgcatttg aaataaaaag
     1501 aactcttcaa aaacgacacg tcgtttggct tgatggtttt cttcgtggtc ttttggaaaa
     1561 tgcatacttc ttacagcaga agctcggtca agcagtcgat atttctccac aaggttgttt
     1621 ggcagatttt cttcaatcaa tgtgccgtat tcttgaaacc cttttttgat tagatcaact
     1681 aatgtcgttt gacgaattga tttgttgacg tgatatatcg gcgaaaagtc atccattgtt
     1741 tgagaaccta aaatcttcat cccgttcaat gctttacgct ttgcatccca tttaccgtat
     1801 acggcgattt cctccgataa aatgactttt tcttttagat aaggttgatt aaaaaaagaa
     1861 acgttgaaca catcatgttc ttgcatcata cgaaattgca atcggctttt tttataacca
     1921 aatcgattca tcacaggagg cgagactacg attcctttca aagtaacctt ttcttgatct
     1981 tggatctcgt ttagatttct ttcctgaata tcttcgtagc ggaaaggata ataagtcagt
     2041 aagtcttcaa tcgtttggat gcccaagctg gcaagactag ctgctctttt ttctccgact
     2101 cctgccagca tagtgattgg atcatctaaa gaacgcataa atttttcctc cctcccttaa
     2161 caaaaaaagg acttggtcag cgatcatacc tgatcactaa ccgaaggcct tttatgaatg
     2221 aatactgtgt tagaaagata ttctgacaca gaaattattc agcagaaaat agataaggat
     2281 agacaggctg atctccttgg tggatctcga tttccaaatc aggatgtttt tctgttagtt
     2341 tctcagcaaa ttcatttgct tgttcaactg ttccttcatc acctgccaag atcgttacga
     2401 tctctgtgtc ctcattgatc atttgttcta gtgtacttaa cgctgttgct gtgatatctg
     2461 ggttagagat caggattttt ccatcgatca tgcccaaaaa gtctccttct ttgatctcta
     2521 caccgtcaat tgaggtatct ctgatcgcgt gagtgacttg accgctcaca acgctagaca
     2581 acatttctgt catcgtttct ttgttttctt ctaaagaagc ttgatcattg aatgaaagca
     2641 tagctgtcaa tccttgggaa atcgttcttg aaggaacaac tgctactggg atatctgcta
     2701 cttctgctgc ttgatcagct gccatgaaga tgttcttatt gtttggcaag acaatcactt
     2761 tatctgcgtt tacttcttga atcgctttaa caatgtcttc cgtacttgga ttcattgttt
     2821 gcccaccact gataacataa ttagcgccta aactttcaaa cagctcttgg acgcctttac
     2881 cagcagcgat cgctacgata ccatacggga tacgtggtgt ttcttttttc gcaaactcgt
     2941 tcacttcttc atcgtgttcc aaaatcgtct catgctgcaa gcgcatgttg tcgactttga
     3001 ttttgactaa tgaaccgaat ttttgtccat aattcaagac ttctcctggg ttttctgtat
     3061 gcacgtggac tttgatgatc tcgtcatcat tgaccacaag taaagaatct cctataccat
     3121 ctaaatagtt acggaattct tcgtaatcga atgtactatc gactgtaggt ccttctccga
     3181 tttttaccat gatttctgta cagtaaccaa aatggatatc ttctgtcgcc atttgacctt
     3241 ggacactgcg atgatgttct gcattgacca tatcatccat ttctgctgga gaaggttcat
     3301 atgtttcatc cgcttggaat tctccattca atgcacttaa aaagccctca taaacaaaca
     3361 gcagtccttg ccctccactg tctacgacac cgacttcttt taggactgga agcaagtctg
     3421 gagttttagc taaagcacgc tttgcccctt ttacaacagc tgtcattact tcaacgacat
     3481 catcagtact ttttgctttc ttttctccag ctttcgctgc ttctctagct actgtcaaaa
     3541 tcgttccttc tactggtttc attactgctt tatacgcagt ttcaactcca tgagtgaagg
     3601 ctgcagcaag atcggaagca tttaatgaga caacatctgg aatttgctta gaaaatccac
     3661 gaaacaattg agacaaaata acgccggaat ttccgcgcgc acccatcagc aatccttttg
     3721 aaagcattgc tgctaattct cctacttttt ctgaggttga atctactact gccttagctc
     3781 cactagtcat agatagattc atatttgttc ccgtatcgcc atctggcaca gggaatacat
     3841 ttaatgaatt gacgtattct gcatttactt gcagacgatt cgcacctgcc tggaccattt
     3901 cctggaactg gcttgcactt atttctgtta cactcaccta gataatcctc cttcagatcg
     3961 actgctgagt taccagccag tctcctgcct agtctggcag tacacgaaca ccttgtacaa
     4021 agacattaac tgaattggca gttacgccta ataatgcctc tagattgtat ttcacttttt
     4081 cttggacgtt gcgggaaact tcagagattt tcgtaccata gctgataatc gtataaacat
     4141 ctacagcaat accattatct tcttggcgga caactactcc tcttgagtaa ttttctttac
     4201 gcaaaatctc agtaatatta tctttaattt gatttttgct ggccattcct acaacaccga
     4261 acacatctgt cgcggcgcca ccaacaactg ttgcaattac atcattggta atttcaatgg
     4321 ttccagctga cgtttgaatt tttacagcca tgaatgagcc tccttaacgg gtaaaaaact
     4381 cccatttttc ttctatcata ttttatcata gcgcaacttg aaataaaagg cagttaccca
     4441 aagtactcaa aaaccttgca aaatttcagg tataaccttg tttaatttac aattattctt
     4501 ctcaattgtc aattgtatct atgaaaatgt acttgcaaat ttccattcta tatgatatgt
     4561 tagtctggta tgagctgaat tattctattg ctccaaatca aggcaaagga ggaaattacc
     4621 atggcaaaag tttgttactt tacaggtcgc aagacaaaaa gtggaaacaa ccgttcacac
     4681 gctatgaact caacaaaacg tactgttaaa cctaacttac aaaaagttcg cgttatggtc
     4741 gacggaaaac ctaaaaaagt ttgggtgtca actcgtgctt tgaaatcagg aaaagttgaa
     4801 cgcgtttaat tgaatgatac ttggaccgtt cagatagtga acggttttt
//
LOCUS       DACDHL010000142         4767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDHL010000142 DACDHL010000000
VERSION     DACDHL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.146"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(120..896)
                     /locus_tag="IV228_002699"
     CDS             complement(120..896)
                     /locus_tag="IV228_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HAP8718539.1"
                     /translation="MSKESMIKWMQDRLGKVTYSMTHRLGPNSYDCSSAVFFSMIAGG
                     FLPSGSMGNTETLFGMVGTKLKKISRSEVQRGDIFISGTPGGSNGSAGHTGIFLSNSS
                     FIHCSYTHNGIAVDTNDAYMSTKLQHNFYRILDGGTANTDNKPQMIQLALDGQFGNAT
                     ARRLQEYFDTAGKDGVISHQFKQTFNQNIYAAQFDNTLTGSNVIKALQGFLGIAQDGL
                     CGQGTIKALQRHLGTTQDGVISPVSNAVKELQRRLNANKL"
     gene            complement(899..1303)
                     /locus_tag="IV228_002700"
     CDS             complement(899..1303)
                     /locus_tag="IV228_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8718540.1"
                     /translation="MVIIDNLVLLSEFKNLVSNVYIQIFVWIVIADIITGVCKGLAGK
                     ETNSTKGLMGVVKHLLVVALVLIAYPYLKIMNFEGVATAFVLSYIAVYGISVIENLGQ
                     LGIPIPDFVKDRFSKLKDSTEKQGKNKKEGGQ"
     gene            complement(1604..1738)
                     /locus_tag="IV228_002701"
     CDS             complement(1604..1738)
                     /locus_tag="IV228_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8718541.1"
                     /translation="MYSYDDIKMMYDWNCFTADQVRQFVPLCITDEEAQKIINKTKVL
                     "
     gene            complement(1740..2147)
                     /locus_tag="IV228_002702"
     CDS             complement(1740..2147)
                     /locus_tag="IV228_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718542.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKANEELTTK
                     AESLAQINSKTMLTSLQNTKDIATIKEQLDGGK"
     gene            complement(2175..2699)
                     /locus_tag="IV228_002703"
     CDS             complement(2175..2699)
                     /locus_tag="IV228_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718543.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCNTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQNSILQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            complement(2699..3067)
                     /locus_tag="IV228_002704"
     CDS             complement(2699..3067)
                     /locus_tag="IV228_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718544.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     VGTVTSGTTASVTNAGTASAAKFNFVLPKGDKGDKGDPGANATTTAVATTTANGLMSK
                     EDKVKLDGLANITFEKVGTV"
     gene            complement(3071..3691)
                     /locus_tag="IV228_002705"
     CDS             complement(3071..3691)
                     /locus_tag="IV228_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718545.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3691..4599)
                     /locus_tag="IV228_002706"
     CDS             complement(3691..4599)
                     /locus_tag="IV228_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8718546.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(4612..>4767)
                     /locus_tag="IV228_002707"
     CDS             complement(4612..>4767)
                     /locus_tag="IV228_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8718547.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
BASE COUNT         1309 a         1012 c          740 g         1706 t
ORIGIN      
        1 atagggttta aagctgattt taagtgtttt tcgcttatta tcagcttttt attatctaaa
       61 aaaacaactt ttagagacat ccatatactg ccagggggag tcaaaagggg agtcattttt
      121 tatagtttat tcgcatttaa tcgacgctgt aattctttca ctgcattaga tacaggactg
      181 attacaccgt cttgtgtggt gcctaaatgt ctttgtaatg ctttgattgt tccttgacca
      241 cacaatccgt cttgagcaat acctaagaaa ccttgtaaag ctttgatgac attcgaccca
      301 gttaatgtat tgtcaaattg agcagcgtaa atattttgat taaatgtttg tttgaactga
      361 tgactgatca caccatcttt gccagctgtg tcaaagtatt cttgtaaacg tctagctgtt
      421 gcattgccaa actgaccatc aagagctaat tgaatcatct gtggtttatt gtctgtatta
      481 gcagttcctc catcgagaat acggtagaaa ttatgttgca gttttgtact catgtatgca
      541 tcattagtat ctactgcgat cccgttatgc gtataagagc aatggataaa tgagctgtta
      601 cttagaaaaa tacctgtatg cccagctgat ccatttgaac caccaggagt acctgaaata
      661 aagatatccc ctctttgtac ttctgatcga ctaatctttt tcagtttcgt tccaaccata
      721 ccgaacaacg tttcagtatt tcccattgat ccagaaggta gaaagcctcc agcaatcatt
      781 gagaagaaaa ctgcagagct gcaatcgtaa gaattaggtc caagccgatg ggtcattgaa
      841 tacgtcactt tacctagtcg atcctgcatc catttaatca ttgactcttt actcattttt
      901 attgtcctcc ttcttttttg tttttccctt gtttttcagt tgaatctttt agcttgctga
      961 atcgatcttt cacaaaatct ggaatcggga tccctagctg acctaaattc tcaatcaccg
     1021 agattccata aacagcaatg tacgaaagca caaaagctgt tgcgactcct tcaaagttca
     1081 tgattttaag ataggggtaa gcaatcagta caagcgccac gaccagcaaa tgttttacta
     1141 cccccattag tcctttagta ctatttgttt cttttccagc aagtccctta caaactcctg
     1201 taatgatatc cgcaatgaca atccaaacaa aaatttgaat ataaacgttg cttactagat
     1261 ttttaaactc tgataacaaa actaaattat caatgatcac catgtttcca cccttcactt
     1321 tctatttttg gccatacaaa aagcgcactc gttcgagtac gctatagtga ctaactcact
     1381 tgtggatgtt tacaattgct cgttttgaaa ccttattacc attaaatttt gttacaagat
     1441 aaattcttgt aattcttgaa ttttcttctg tttctaataa ccaacttggt ttgaatgcta
     1501 acacttttat cccctccttt cttcttcgat agtaatatag cacaacacgt ctaaaatacg
     1561 atgcgtataa agaaaaagtt tatttatatt aactatgttg ttatcacaac actttagttt
     1621 tattaataat tttttgtgct tcttcgtccg taatacataa tggaacgaat tgtcgaactt
     1681 ggtcagcagt aaaacaattc cagtcataca tcatcttgat atcatcataa gaatacatat
     1741 tattttcctc cgtcaagttg ttctttgata gtagcaatgt cttttgtgtt ttgaagggat
     1801 gtcagcatgg ttttcgaatt aatttgtgct agtgattctg cttttgtagt aagctcttca
     1861 tttgcttttt tcaatgcagc attatctacc tgtaaaccta tagataaatt ttctagtagt
     1921 tctaattttt ttgaataatc ttgtgtaact gcttcttccc acttattttc agtgaagtta
     1981 aaaaattgcg actgttgtct tcttccgaaa ccttcatccg tttcatcttc cttcttttca
     2041 aacaaaatag gtggaacttc gacaaatggt aaagatgttg gaaagttatc ttctacttga
     2101 tgttcttcat atcccattgg atacaatact ttataaattg ttttcatttt actcttcctc
     2161 caattagtaa tctgttaagc cggccaagga tcttgagtga tccacatacc tgaaatatat
     2221 gaactacctg aacctgattt ggcttgtagg atgctatttt gatcaatgaa tgcacgtgca
     2281 tccgctggct gacttgcatt tctaactagt gaaagcgcgg tttgcgctgg atagccttga
     2341 tcacgtttga aaccgtccgg gatttttaaa atccaacgag tttctgatcc ttcgggccat
     2401 gtgttacatt taaaattaaa agtaagaaat acgatgtttc cgattctgat tatttttcca
     2461 ttcacatcag ttacgtttgt tgtatcactt cgatcagcta gtgtgatagc tcgctcgatc
     2521 atacctgctt gcacaggcaa tccattgaat tgcaatccgt ctttaaaatt tttcgttcct
     2581 aagaccgttt cattacctgt ggcttttact aaaacaccct ccacaccatc aattcctttg
     2641 gcatgagttt tcaaatactt agcaactccg tcttctttta attgcacaat atcagccatt
     2701 aaaccgtccc cactttctca aatgtaatat tggctaatcc atcgagtttc actttatctt
     2761 ctttggacat caaaccattt gctgtcgtcg tagccacagc tgtcgttgtt gcattggctc
     2821 caggatcgcc tttgtcccct ttatcacctt ttggcaatac aaaattaaac tttgctgcac
     2881 ttgcagtacc agcatttgtg acactagctg ttgtaccact cgttactgtc cctacagtaa
     2941 ttgtggcagc ctgtccaggg tctcctttat ctcccttcac cgttgttggt ttgccttcta
     3001 tagcattcca atgagtttgt ggataaacct gtacattgct ttgttttatt tttacgatat
     3061 ctgtcattct ctatacctcc ccaatttttt caaaagtaaa atttggaatt ctttcgtttg
     3121 tgtaattttc tgcttgattt acagcttctt ggaatttttg atctacatat gactgattga
     3181 caccaccagt cccactacca cctgtagaac taattgttcc atcttcatca atagtgatgt
     3241 tttctccagc agtataattt ttcagtgaat cgagtttttc ttttaactcc agcgaaaaat
     3301 tgacatcaga ttgtctgact gcagagactt tccctgtctc atcaatcgtc aataattcac
     3361 caacttttat gccacccaaa cggtcgatag tggcaactgg caacacgtag tcaccttctc
     3421 cactgtttaa aattttttga tacatttctg ccgtgataat gccgtcatct tcttcacttg
     3481 catagggtaa ttctgtgaga acattctcta atcccaaatc ttctttagta atgacgacgg
     3541 ctccaatttt tccattaaca gataaaactg ttgcttctcc tgctattatt tcttctaacc
     3601 caagaactgc ggaagcatgt gtgattggaa agaattggcg ttgtacacct gttttttcat
     3661 ccgtttccat cattcgttta gttttagcca ttagatcacc ccgactttct caagagtgaa
     3721 cacattttgc tttggatcat caacggttgc gatgatcaag gctccttctt caattggaaa
     3781 ttctactgtt tcaatttttt cgacttcgtg attatctgaa aagagatcgt cttgaagaat
     3841 atcagtcact tcaatttcac cgtattcaat cgtgaaaagt gctgctttca ttttttgata
     3901 caaataattt agatcggata ggaaacgttc agaaatcgat tcatggcgta caccttgaag
     3961 atctacacgt gcatccatta attcggctaa cattactcca cctggatcga tagacttcaa
     4021 aatatccttg attgattcaa accatgacaa ataatctgat tcttgtcctt ctctccaatc
     4081 ttggaaacta ttctcctgtt cttctctcca gcgatcaaat tcttctttcc tttcattcat
     4141 ccagtccgta aaatcgcctt tattttcatt aataaaatct gtcatatcag caattaaatc
     4201 ttcaattgat tgccaataag aacccatttc tccttctgtt tttgaaacag cattgatgac
     4261 aaaataggag aagtcttgag ttgttccaat caagtcttct cctttaaata taatgaaatt
     4321 ggctgtttgt cgatgcaaac actgcatgga atatttatca aaaatatatt tgattttccc
     4381 ttttttagca tccacaattc ttgtttctaa ttggactgga tattttccgc caacaactga
     4441 ttcaaaatat accttacatt ctgataaatc atatggaaga ccattttcga caattgtagc
     4501 ttccataact tcagtgtttt tattgccttg tcgaacttga atcatcccca cgtaattata
     4561 gggttctgtt gtacttaata tgacattcca ctttgccatt aaatcacctc cctattttgg
     4621 tggtatgaca atggaagaaa ttgcacttgc actataatac tggcgatcta actttccaca
     4681 aatcatacct aactctgtgt tttgttcata agtttgcata cgaccattag ctaagcctcg
     4741 gataacgcca gtatgtccat aagttcc
//
LOCUS       DACDHL010000143         4744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDHL010000143 DACDHL010000000
VERSION     DACDHL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.147"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(28..1026)
                     /locus_tag="IV228_002708"
     CDS             complement(28..1026)
                     /locus_tag="IV228_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8718548.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1101..1397)
                     /locus_tag="IV228_002709"
     CDS             complement(1101..1397)
                     /locus_tag="IV228_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718549.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1390..1683)
                     /locus_tag="IV228_002710"
     CDS             complement(1390..1683)
                     /locus_tag="IV228_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718550.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1829..2380)
                     /locus_tag="IV228_002711"
     CDS             complement(1829..2380)
                     /locus_tag="IV228_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8718551.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2554..2853)
                     /locus_tag="IV228_002712"
     CDS             complement(2554..2853)
                     /locus_tag="IV228_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8718552.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2880..3146)
                     /locus_tag="IV228_002713"
     CDS             complement(2880..3146)
                     /locus_tag="IV228_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8718553.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            3433..4176
                     /locus_tag="IV228_002714"
     CDS             3433..4176
                     /locus_tag="IV228_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718554.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(4213..4710)
                     /locus_tag="IV228_002715"
     CDS             complement(4213..4710)
                     /locus_tag="IV228_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8718555.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1404 a          907 c          694 g         1739 t
ORIGIN      
        1 tttttctttg ataaataata ttttcaatta tttaatcatt tgaaaattgt tagtcattcc
       61 aatacgttga gaaattcttc catctgtata aacacgaaat tgttttccac cagccatggt
      121 cgtgtttcca tcatcatccg ttacaatatt aattgaatag tagcttccat tttcatcttg
      181 cattagatct ttcatatatt catttgtgct aattaaatta gtatttgata catttttatc
      241 aggagaatca tacaaccata aagctttatt atttagcaca gattctttag cagcttccca
      301 acttgaaatt cctgaactac ttgtagattg tgtcgttgta gttgttgctt gttgagtaga
      361 ttcttctact actggtgttt gaggagtttg tccattcaca atatcactga aaccttgttt
      421 taactgttga ttttgagttt cgttaaagta cagataatta ttttcattac cttgattttg
      481 ttgcgttaaa taaactttag gttgtccatt ttgaaaaccg aaaaagtaaa catgctgccc
      541 taaatattct ccagtttcta catcagaata aactgctact aaattgaaat ctgcatgacc
      601 agttccatct tctgaccatt gtacggtaac tggtgcttca ttgattgcca ttttccaatc
      661 gccattaatc acggcttgtg gtaaaggagt gccatacaga cttacattat tctgaagatt
      721 gtagctttta tattcttgac ctaatgtctt tccccattgt gtgacaaaag tttctaattt
      781 ggaagcttta ttcgtatccc acaaagtatt cttaacctct tcagaagaag attcagtgat
      841 tgaacttgca cttgtttcca cagattgctt catttctgtt gtagtttgtt cagttgattg
      901 cgttttactt tttttagtct caattgttgt atcagtttct atagtcgctt tagtcctcgt
      961 attctcagat gtacaaccag tgaataaaat tcccattcct attagtaata acagccattt
     1021 tttcattata tttccccctt tcaatttctc cctcacgtac ttattctatc agacaattaa
     1081 ttgatctaaa tggtaatctt ttaatcgatt ctttcccaat aatgtcgata gcccgtatgg
     1141 cagcggtctc ttccttgagg actctcagat ggattaaaac tagtagaaat ttcccttcca
     1201 catcctctac agcgataagt tctcattatt tttgggctat tagtattatt tttcttagtt
     1261 agagctatga gtccaactat gattacccca actgtcttaa ttacttttcc tttatctata
     1321 ttcggatgcg caagcatata ttgatataac caatatccac cgtaaattaa accacctaat
     1381 acaatcagct tagccatgta ctcgtctcct ttctttttgt ttaattactt tttgaatcca
     1441 tcttgcagag aaaccataaa cttctgtcaa ttgacggata tctacagttt tttcctgttc
     1501 tatcatgttt tcgattcttt ttctaactaa ctcacgatcg tacattttca ttggaaaaga
     1561 aatctgttgc cctcgaaaag caagataaaa ttgatagagc aattctgaat ccagttccat
     1621 ttcctcaaaa aagtcattca tacttttata agagttactc aaacaagttt catcaatctc
     1681 catctacaca ccccctgtta ttttttacat aatgaaaata gcatattact gggtacattg
     1741 ataattcata taacatgtat catcacatgt atttttaaag tcaaaaagca tgtaaaacta
     1801 cttataagta acatgagaaa ataaaatgtt atttttcttg aaataataat tgactatctg
     1861 ccaattgcca ttcattacga acatgaatac ttagtgggtg ggataactta ccttctgcat
     1921 ttatcaatac tttggtcatc ttcttttgtt tcgcactcaa ttgatcgtaa atagtctttg
     1981 cttgttcata agccacttga ctcgtcgctg taatcgaacc acaagcaaaa ataatggtat
     2041 tgattttctt ttctgtaaaa acagtcgtta atatttccaa agattgttca tcatttcctc
     2101 gaagaaatac ccgaggactt ttcgcttttg tgtacgaaaa aagattcaca gcaataaaac
     2161 cagtaaatcc taattgtcgg atattctttt cgattaataa catactcaaa tcattcgtaa
     2221 atggttctgt atctgttgga cgaatagtaa tgactactgc taatttatct tccgcttctt
     2281 ttcctttgat cgcccattgt cgtttgaaaa catagcgatg ggtatgatga ggatctcgcc
     2341 aaacttctgt ttgtgtggtc actaattctt tttcaatcat ctctttttct cctatgctat
     2401 tttttgttca agcacttcaa ttaccattgc cgttataagt gtcaatacta gttgaaaata
     2461 tttcatattc tttctccttt tttcttcaga atttgatgaa tcgcggtaag gatatcttct
     2521 aaacatttcg caatcattct caaataatcg atcttattca tccgttcctc caatctgact
     2581 ccaaaccaat cgtccttcta ccagtaaccc acgaacaaat tctcgctggc tggtaatatc
     2641 tgtaatgaaa taaactacat agtcttttcc atcttctggt gcgcaataaa tataaagatg
     2701 tgtcatcact aaacgtggta gatgaacatc ttcaggaagc atacccattt caatttcctc
     2761 tttttccatc gtaacggatt catagcccca ctcttcagaa gcagtgacca gctgtgcaca
     2821 cttttccagt acttcgatag ccgtttctaa caagtccttt cgttcctttc ttttagatac
     2881 tagtattctt ctggtaataa catcgtttga tactcacccg aatcaatggc ccatattttt
     2941 tgagacactg atttcaaacg aagcttaggt gaaatcagat actcttttct ttcttccggt
     3001 tcttcttgac gatgaaccaa cttttgattt cctttttccg tagaaaattc aaaaatttga
     3061 agatagtcta aaggaaattg cttttgaacg ttttgatcga ttagattcca acataaagct
     3121 tgtaattcca ccggcaaact gtccattact ccctttgtga cataacgtta tcctttattt
     3181 tgaaacattt cttttccctc ctttgccata aaaatagccc aatcttctat taaagaagat
     3241 tgggcttcat ggatataata tatatttgta aataaagtta attcagttat aattatcaaa
     3301 actacacaat tattaaagtt tttcatatct ctttgtttag taagtttttc taaatcttaa
     3361 cccgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg gtaaatcaac
     3421 aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg aggactattt aacaaactac
     3481 gatggttacg tcgtgacaac tgaacatttt gacttagcca atcctgttac taaagtcgat
     3541 gatatccccg attatgaaat gtacagtcaa acaatcgata gcttaaataa gcgttttggc
     3601 aatcgtgttt taaaaggcat caaaatcggt tacatcgctt ctgaaaagga tcggatcatc
     3661 gactatttag ccgataaaga ttacgattta aaattactca gtgtgcatca taatgggcaa
     3721 tttgattatt tagatgacga agtcaaagat atggatcctg ctattgtgat tcctcagtat
     3781 tttgcacaac tttcagaagc actagttgtt atagaagcag atgtctttgc tcattttgac
     3841 tatggttttc gggtatttgg tctatccgtt gctgaattta aacaatatga agcgcagttt
     3901 ttaccgattc tagatcaagt aatcaaaaat aaattggcat tcgaattaaa tgctaagagc
     3961 gcttacttgt atgacaattt agcgttatat gaatatgtca tcgatttata tctttcacgt
     4021 ggtggtacct tgtttagtgt tggatcagat gggcattacc ttgaacattt ccgtttccat
     4081 ttcgatgatc tttttgctct gttaaaagca aatggtgtga cggaattagc gatttatcaa
     4141 aaaggaaagc gaattatggt tccattacca gtctaaaaaa acgtcctcag catcaaaagt
     4201 gaggacgttt ttttagattt ctttttctaa aatcggacaa atacgatctt gttttcctaa
     4261 aggaaaatgt ttgatcccga tttttttaaa tccatgacgt tcataaaaac gaatacctgc
     4321 atgatttttt tcataaacag tcaaacgtaa ataacgtttt tgtttcgttt tagcataacg
     4381 attcgcttca tcgagtaacc gttgccctaa ccctttgttt tgataatctt tgagcagata
     4441 gagccgttga atctctatag catctttttc atcattgact ttcatataac caatgagtat
     4501 ttgatgatcc ttaataccaa taaatttact ggttgactgt gccaattcta ctgccaatat
     4561 ttcagttgtc aattgtgtct gtaaatatcc ttgcatatca agtggatctg cgccatcgat
     4621 aaacgattca taaaaactga caacacttac aacttgtaaa gcatctagat catcataatc
     4681 taaatacgct agtgtgattt gattgttcat ctaactcctc cttttgacat agtatactat
     4741 aaat
//
LOCUS       DACDHL010000144         4725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDHL010000144 DACDHL010000000
VERSION     DACDHL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.148"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            351..2195
                     /locus_tag="IV228_002716"
     CDS             351..2195
                     /locus_tag="IV228_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8718556.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2251..3717
                     /locus_tag="IV228_002717"
     CDS             2251..3717
                     /locus_tag="IV228_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8718557.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3754..4596
                     /locus_tag="IV228_002718"
     CDS             3754..4596
                     /locus_tag="IV228_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8718558.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1543 a          759 c         1012 g         1411 t
ORIGIN      
        1 tctgctagaa gatagaagag aataagagtt atatatgttg gaagatattc ttagaaaaaa
       61 gacaaaactt ttagaacacg aaaactatat tgaacgaata aaatgaaaaa atgattaaaa
      121 aatcatttta ttatttacgt ttacattcta atatgttaaa ttgaatataa cttaaacagg
      181 catgtgactg gtaatgcagg caggacctaa ataagttgaa taacaatgta tgcgtttcaa
      241 tacacgtttg ccttgttgtc aatttattta gtttctgcct gttttttgtg ttgaggatgg
      301 ccacttcgac tcaaaagttc atcactcaaa aaagaaaaga gggaaacacc atgaattaca
      361 aacaaacagc agaagaagta ttgcagtaca tcggtgggga agaaaacata tcccatttgg
      421 agcattgctc aactcgttta cgttttacgt taaaagacaa cagcaaggta aatatgagcc
      481 agttagaaaa agtggatggc gttatgggcg ttcgccaaaa tgtgcaatgc caagtaatca
      541 ttggaaacga tgttgttgaa gtgtatgatg aagtgaaagc gttattaggt gacaatgttt
      601 caaatactga agaagcacca aaacaaaaac aaaaattagg ttcagttcta ttagatttta
      661 ttatttctat tttccaacct ttaattcctg cgattgcagg tggaggagta ttgaagtcct
      721 tgttgctact agcttctatc ttagggctga ttagtgacca atctcaaacc tatcagattt
      781 taaatttggt cggcggggca ccgctttatt tcttgcctat tctggtggca atgactacgg
      841 ctaataaatt gaaagtcaat caattggttg cagtatctgc tgtcggtgca ttgatccttc
      901 ctgaaatgac agctatgttg gcggaaggtg ctacattctt atcttttgga ttggataata
      961 tagcgtatgc atctcaagta ttcccagcca tcttaactgt tttattttat gctcaacttg
     1021 aaaaatggtt tacaaaagtt tcaccaaaac caattcgtat tttctttgta cctatgatga
     1081 gcttattgat tacagttcct attgctttgt tgcttctagg accattaggt tataatgtag
     1141 gaacagcttt ctcttctatc attgtgtctt tgtacaatca atttggatgg gttgcaaccg
     1201 gagtcttagc agctgtactg ccattaatgg ttgtaacggg tatgcataaa gcaatgatcc
     1261 cttacgctgt ttcatccatg agtgaattgg gtgcagaact tctttatctc ccggcttcat
     1321 tggcacacaa tatttcagaa gctggcgcat gctttgctgt cagtatcaaa acaaaagaca
     1381 aaaaattgaa atctacggct gtttctgcgg gtatatctgc attattcgga attactgaac
     1441 cagctcttta cggggtaacg attctgcaca aacgtgtttt atatgccgtt atgttttcta
     1501 gcctgatcgg gggagcattt gcaggaatcg ttgcacttca agcctttgct ttagtgggtc
     1561 ctgggattgc aagtatcacg atgttcgctg acaaagctga cccaatgaat ctggtttggg
     1621 catttgcagt atcggggatt tcatttgtgg ttgcttttgc tgcagcgttg gttctttata
     1681 aagataaaga agttgtcaat gaagttgaaa ctaagatgga aacggccgtt gtttcagatg
     1741 ctgttctttc aagtcccgtg aatggaaaag taatctcact atcagaagta aaagacactg
     1801 ttttttctag tggtatggta ggtgatggag tagcaatcat tcctgaagaa gggatattag
     1861 tagctcctga agatggagaa atcacaatgg tatttgaaac gaagcatgct attggtttaa
     1921 aaacggctaa tggtgcagaa cttctttttc atattggtat tgatactgtt cagttagaag
     1981 gaaaagggtt tgaggcattt gttcgttctg gtgaaaaagt agccaaagga caaaagctga
     2041 ttgcatttga tattgattta atcaaagaag caggattcga ccccacagtt atctgtgtgg
     2101 taaccaataa ggataattac actatccgtt catttaacct agaaaaattg gtaaccaatg
     2161 atagtgatct cttagctatc agtccgatag cttaaacaca agatagtgaa aaaaaatata
     2221 ttgaatgaag gaatagagga gaatagaata atgacaaaca caacaagttt tccaaaagga
     2281 tttttatggg gcggcgcgat agcagcaaat caagtagaag gtgcttggaa tgtagacggt
     2341 aaaggtcttt cagtagcgga tatggctact tataaaccaa atttagacaa caaagatttt
     2401 gctgctcata tgcatgtcac cctgcagtct ataaaagaag ctagtgaaga caaatctgat
     2461 aaatggtacc caaaaagaag gggcgttgat ttttatcatc actataaaga agatttagct
     2521 ttatttgccg aaatgggctt taaaaccttg cgcctctcta ttgcttggac tcgcttgttc
     2581 cctacaggag aagagctgga accgaatgaa aaaggggttg cattttatga aaacgtcttt
     2641 aaagaaatgc aacgtttagg aattgagccg attgtgacac tatctcatta tgaaatgcca
     2701 ttagctttag cagttaaata taatggctgg gtggagcgtc gagtaattga tgattttgtt
     2761 cgcttcgcta acgtttgttt tgatcgcttc ggcaaatacg taaaatattg gctgacattt
     2821 aatgagatag acagcatcca ccgtcatcca tttacaacag ctggaattat ccaagagaaa
     2881 agcgcagaag gtcaagaagc tcaagatatc tatcaagcat tgcatcatca atttgttgcg
     2941 agtgcgttag tgacaaaaga tgctcatgaa aaaattccag gaagccaagt tggttgtatg
     3001 ttaacgaaat tgatgactta tccattaact tgcgcaccag aagacgttga gctgaccttg
     3061 aaaaagaact tagaaaataa tttttatgca gatgttcaag ttaagggaga atatcctgct
     3121 ctaatcgaaa aggatttgga aaatcgggga attcagatta aaatggaaga tgaagatcga
     3181 gaaattttaa agaaacatac cgtagatttt gtttctttca gctattacat gtccatggca
     3241 gaatcagctg atccaaacgc agaacgcaca ccgggaaata ctgttttagg ggttaagaat
     3301 cctcatttgc cttcaacaga ttggggctgg caaatcgatc caaaaggttt gaaaatttcg
     3361 ttgatcgaac tttatgatcg ttaccaagta ccgttgatga ttgtggaaaa tggtatggga
     3421 gcagtcgatc aggtagtaga tggtaaaatc catgatccgt accgcatcga ctactttaaa
     3481 gcacacttcc aacaaatgaa agaagcaatc gatgaaggtg tagatttgat agcttatact
     3541 agctggggac caattgattt agttagtgca ggaacttctc aaatgtctaa acgatatgga
     3601 tttatttatg tggatgcaga cgatgaagga aatgggacat ataaccgctt gaaaaaggat
     3661 tcgttcaact ggtacaaaga agtgatcgaa acaaacggtg catccttatt cgagtagtct
     3721 cgatcttttt aagcagcgat agggggataa tgagtgattt caataaaaaa agtattgaat
     3781 tcaagtgtgg tacttgttga agaaaatggc aaagaaatga ttgcattagg taaagggata
     3841 ggctttggac agaaagctgg ggatgaaata tcaggagata aagtagataa aatatttctg
     3901 ccttttgaag aaaagaaatc ttcacaacta ttagagttag ttgaagaaat acctattgaa
     3961 ttttttgaaa tcactcgtac tatcgttttt gaagcagaaa aaggattaga taaaaaactg
     4021 aatcctggag tctatttaac cttaacagac cacctgcatt ttgcagttga acgcgcacaa
     4081 aaaggggtaa atattgcaaa ccgtttgtac tgggaaataa aaatctacta tcccaaagaa
     4141 tttcaaattg ggaagtacgc actcaccctt ttgaacgagc aatatggaat tgatcttcct
     4201 aaagaggaag cctcaaacat tgctttccat ttgatcaatg cacaatcgga agatcctgat
     4261 agcggcgctg gattcaagta tgcaaaaatg atcggcggga tcgtcaattt ggtccggtat
     4321 tcgttgcaga aagatatcga tactaattcg atccattatg cccgcttcat tacacatgta
     4381 cgtttctttg tagaaagata ttattctgat gctttgttgg atgatggtga ggaagaattc
     4441 tacaatcaaa tgtggatctt atacccgtct gccatggaaa gcgcagctaa gatcaaagac
     4501 tacattgaaa aagtttatca taaaacaatt ccgaacgatg aagttgtata cctaggtgtc
     4561 cacatcaatc ggttgatgaa acatgctaat gggtgagcgc tcagtaatac tattgatgct
     4621 tttaaaatct ttagagatgg attatagtaa aaacaaggtg atttccagct gtttgttatc
     4681 tataagagac atttaaaaag gattaacgta agataataaa aaatc
//
LOCUS       DACDHL010000145         4649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDHL010000145 DACDHL010000000
VERSION     DACDHL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.149"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(271..2043)
                     /locus_tag="IV228_002719"
     CDS             complement(271..2043)
                     /locus_tag="IV228_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718559.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1997..3751)
                     /locus_tag="IV228_002720"
     CDS             complement(1997..3751)
                     /locus_tag="IV228_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718560.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3861..4430)
                     /locus_tag="IV228_002721"
     CDS             complement(3861..4430)
                     /locus_tag="IV228_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8718561.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4448..>4649)
                     /locus_tag="IV228_002722"
     CDS             complement(4448..>4649)
                     /locus_tag="IV228_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718562.1"
                     /translation="NMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIK
                     KDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1560 a          868 c          631 g         1590 t
ORIGIN      
        1 cgttttaagt agcttttcct ctacttaaaa cggcaatatg aaagaaacta taacttttta
       61 ttcaatcttt tatgtttaac tggtttatcg ccaatttttt aaaactttga ttattcctta
      121 tattataaga atagacatta agtgaactaa ttcaatcata ctgattcttt cgtgtattca
      181 aaattagatg ctgtttaata aaattattct ttttgaaaaa gaattataac tatatcccaa
      241 aaattgtagg ttaatttaaa gcctatttca tcatcgtgat tgagcttctt tttcatagac
      301 gaatttctca gatagtttag ttttctttat gagatttttg tatatttctg accttcgata
      361 caactcacta tgagtacctt gatcaactat ttttccttga tccatcacta caattttatt
      421 taccttttca atagacttca agcgatgcga tataattagt actgtcttat cttgtattaa
      481 acgatttaaa ctttcctgaa tacgtttttc attttccgca tctaaactgg cagtaatttc
      541 atctaaaatt aaaattggcg catttttcaa aaaagcccgt gctatggata ggcgttgtcg
      601 ctctcctcca gaaagtgaac ttccgttttc tccgaccaat gtatgataac cgtcaggtag
      661 tttttcaata aagtcttcgc agttagctaa acgagctgct tttttcactt cctcatctga
      721 cgcttgacta tttccaatac gtatattttc caaaatactt gtattaaaca aagttacctc
      781 ttgaaaaaca atagcgattt tgctaaacaa agaagctgga gatacacgtt taatatcata
      841 cccatcaatt aaaatacagc cttcatcata atcatacagt ctagacacta atttcaagat
      901 actagttttc ccggaaccac tagcacctac taacgcagtc acttctcctt gtttagccgt
      961 aaaagagata tgatctaaaa ttggggtatt gttatcatat gagaaagaga catctcgcaa
     1021 ctcaatgtca aaagatttca gaggactatc tgatccctct tggatactgg tttccttcat
     1081 agctctaatt cgctgtattt taggagctaa ataaaatatt tcaagcactg cttctttcat
     1141 agaatcgaag gaatccttta tttttattgc ggctagtaag taaccaacga cgtagagtat
     1201 agtcacttca cccgttaaca gtaagtgaac acctactaga atgactactg ctaaactaac
     1261 atatgagaaa atagaggata aggccatgac tgaaaaagta cttaattcta cctttaaatg
     1321 aatcctttca ctctcttcca tttttttgaa aagtctgtct tgaacttttt tagatagatt
     1381 aaagctatta atctcttgct gcaattctat agtttcttga aattcttccg agttttctct
     1441 caaagtatcg taatatttag tattggcttt cgtttgggat ttctttgata acaagattaa
     1501 gacaaaacta aataacgttg gcaaaataac agctaatccc attttgacat taccaaccaa
     1561 aagcatcact gaaataaaag ggaaaaacag agccataccg ccggctttag gtattgcatg
     1621 actcatcgca tgctcaatac cttcaacgtc agacatgata gtttgagata aatctgataa
     1681 attatgtttt gaaaaatagg ataatggtaa atttgacaaa tcgtctgcaa tttgcattct
     1741 taaatgcgca ctttctttat aagtactatt atataagttt tcatactccc tatctaacaa
     1801 ccaatacatg aaaagcaaag taacgaacga tactgtaaaa taaagccaag gcggctttac
     1861 attttctaaa actaattcgt ctatcagcat cattataatg aatgctggta ccatattaat
     1921 gcagtataca aaaaagctag aaatagatgc tttaaccaaa tcttctgcgc cctgattagt
     1981 taaagcgaat ttgtttttat aaaaactttt cattgtcaag cctccaatca tttgcattct
     2041 cataattagt gaccaattgt ttatacactt gatgtttagc cattaattca ttatgatttc
     2101 cacgttctac tattttccct tctgacaaga caagaatttc atctacagat ttaatgctgg
     2161 ataatcgatg agctatcata ataactgttt tttctttcat cagatgttga aaagcctttt
     2221 gtagttcata ctcattatct ggatcaatag atgcactggc ttcatccata ataacaatct
     2281 ttgcatcttt taaaattgct ctggcaatag ctatacgttg tttttctcct cctgacagat
     2341 aaactccttt actaccaatt aaggtatgct ctctttcggg aaatttttct aaaatcgatt
     2401 cgcaaccggc taatcttagc gcgtcaaata ctttacttgc tggcgcttga ggatttgcta
     2461 tagaaacgtt atcgtaaata ctctgattga atagtttcgt atcttgaaag acaaaagcaa
     2521 ttgtgttaat aattgtgtct tttgaatagt gatttaaagg cttaccacca attttaatct
     2581 gtcccttatc cacattgtag aaacctgaaa ttagtttagc tatcgtactc tttccgcttc
     2641 ctgattttcc tactaatgca taaattcttt tttgttctaa aactaagttg aaattttcta
     2701 aaacacactc ttctttgtat gaaaagctga cattttcaaa tgcaatgtca caatttttaa
     2761 tctgtgtctc gttcccatat tttagttggt cttgcttcat ttctaaataa agggagtcta
     2821 gtgtttcgac tgcatatctt gcttcaaaca agtgcatact tacatacatt attctcatga
     2881 aagaaacaaa aataattcca cttaaaaaaa acaacatcaa caagtcaaca gctaatatct
     2941 tgggagtggt aagagaagtt ataaaaaagg ttataggaac tatcaaaatg caaactatgc
     3001 caaaaaatag ccattgaaaa agaacaaaag gtgttttccc actcaaagaa tacttatatg
     3061 caaacttagc ataatcgtta attgctttat tcaaggcttt aaatgatgtt actttagcac
     3121 caaatatctt tataacttga attcctcgga tatattctac cgtttcagag cttaacacat
     3181 ctaacgattc ttgatatttt tgaataaaat ttgtattctc tgtcattttc tttaacaaga
     3241 atacaccaat tactaacatg gcgattagaa ccaatccaat tttaaaatta atgaaaaagc
     3301 ctaaggcaac tgataaaaca ggtgttacta tggcttgagc actatctgga atcaagtgag
     3361 caattgccat atgtgtttta gccgcgttgt catcaattgt ctttcgaata tatcccgaag
     3421 aatttaaatc aaaaaaacga aaacttgctt ctgttaaacc gctaattccc ttttttctca
     3481 aattagtctc taagcgaaac ccaaggatat gagaaaataa gccagaaaaa atataaaata
     3541 aagctcctaa agttaaccaa gcaacagttt gaaccgcata actcttagct aaatattcat
     3601 ctcctacaat aattagagac ttaagaaatt tgtaaatact gtaatatcca tagactgtta
     3661 atacgacaga aaaatcagaa aataaagtgg ctaaccatcc gtagtatttc attttaggga
     3721 cataactaaa taattttttg tatactttca ttttttcctc ccccgcattc aatttcttct
     3781 cttattcgtt tacaactgtt aatgctttcc gtaatagcgt aaattagaga tgtacaaaca
     3841 gatagaaggt aaactacgtt ctattttttg tattttttta agacttttct tccaatcaat
     3901 acaccaatca tagataaact tgctacaact aacactgaaa tgattcccca tatacctacc
     3961 gcatagtgga tcattgcatc cattgttgca gcagattgtc ctttatctat ccaatcttga
     4021 cgaaaacgat ctgtaaagaa ccatatagga atccactgtc cggcagcact gcctaattgc
     4081 attagagccc aagacgcaac ttgaggaatt gctcgatcat atccaaatac agatagaata
     4141 atatcagcta agatcgccat actaatcgaa ctgataaacc aaggtaagta cgccccgccc
     4201 attaacgtaa aaaataacaa taaaacaatt gtgtaaatag taaaaattcc ttttctaggg
     4261 tagttatagc taatataaac aaatatgatt ccagataata atccaaaaat ccctggggat
     4321 atgctgtgac caaacgcccc accaatacta ctaaccatta ttgaaaaaaa gtaaattact
     4381 agggaaataa ctgtagctaa accaataaaa atataatctt ttgttttcat atatattctc
     4441 ctcaattcta tttcgttatg tcattaaaaa tctgttctat caaagttgaa tcttctactc
     4501 taagttttat atcttttttt attccaccat tttttaaata aataattcta ttgcatgttt
     4561 tataaagaaa ttccggatca tgagtggcta taactactgc acccttttct tttaagtctt
     4621 taataaggtc agcaatcctt tccatatta
//
LOCUS       DACDHL010000146         4587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDHL010000146 DACDHL010000000
VERSION     DACDHL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.150"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            186..2855
                     /locus_tag="IV228_002723"
     CDS             186..2855
                     /locus_tag="IV228_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8718563.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="IV228_002724"
     CDS             complement(2946..3566)
                     /locus_tag="IV228_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8718564.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="IV228_002725"
     CDS             3680..4219
                     /locus_tag="IV228_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718565.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="IV228_002726"
     CDS             4216..4554
                     /locus_tag="IV228_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP8718566.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DACDHL010000147         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDHL010000147 DACDHL010000000
VERSION     DACDHL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.151"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IV228_002727"
     CDS             574..1008
                     /locus_tag="IV228_002727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718567.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IV228_002728"
     CDS             2298..3221
                     /locus_tag="IV228_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8718568.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IV228_002729"
     CDS             3237..3776
                     /locus_tag="IV228_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8718569.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          674 c          757 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DACDHL010000148         4443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDHL010000148 DACDHL010000000
VERSION     DACDHL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.152"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..482)
                     /locus_tag="IV228_002730"
     CDS             complement(12..482)
                     /locus_tag="IV228_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8718570.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IV228_002731"
     CDS             complement(641..1099)
                     /locus_tag="IV228_002731"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8718571.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IV228_002732"
     CDS             complement(1270..2415)
                     /locus_tag="IV228_002732"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8718572.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IV228_002733"
     CDS             complement(2428..3345)
                     /locus_tag="IV228_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8718573.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IV228_002734"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IV228_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8718574.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1367 a          828 c          848 g         1400 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaa
//
LOCUS       DACDHL010000149         4434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDHL010000149 DACDHL010000000
VERSION     DACDHL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.153"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IV228_002735"
     CDS             complement(543..2048)
                     /locus_tag="IV228_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8718575.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IV228_002736"
     CDS             complement(2045..2731)
                     /locus_tag="IV228_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8718576.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV228_002737"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV228_002737"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8718577.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DACDHL010000150         4386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDHL010000150 DACDHL010000000
VERSION     DACDHL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.154"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..434)
                     /locus_tag="IV228_002738"
     CDS             complement(<1..434)
                     /locus_tag="IV228_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8718578.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFA"
     gene            981..2222
                     /gene="sstT"
                     /locus_tag="IV228_002739"
     CDS             981..2222
                     /gene="sstT"
                     /locus_tag="IV228_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8718579.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            2369..3004
                     /locus_tag="IV228_002740"
     CDS             2369..3004
                     /locus_tag="IV228_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8718580.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            3195..3935
                     /gene="truA"
                     /locus_tag="IV228_002741"
     CDS             3195..3935
                     /gene="truA"
                     /locus_tag="IV228_002741"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8718581.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(4080..>4386)
                     /locus_tag="IV228_002742"
     CDS             complement(4080..>4386)
                     /locus_tag="IV228_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8718582.1"
                     /translation="KLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGT
                     TELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         1392 a          666 c          875 g         1453 t
ORIGIN      
        1 gcgaagagag aatatggttt agaaaacctc agcgctgtat ctgtatttcc tctgatgatg
       61 agtgaaattt gtgtcatcaa aataatcaaa ccgaataaaa caatcaagct gataaatact
      121 tgaggaagct tatagcggac cttttctttc cagttccatt ctttcaaacc ataacccatt
      181 aaaaataaat aaaaaatcca taaaatactc tgtccattct gtaatgcaaa cgggtcttta
      241 ttaaatagag tagagcagcc aactaatcca attgttaaaa ataatagtag atatttaatt
      301 gttgttttcg ataatttatt taaaaatcca actgtataag gaatactaca aaaaagcaaa
      361 gtacaagaaa cagcataagt aaagtaattc tgactgatag gtgttaggat aagccaataa
      421 tcacgaatat tcaagctgcc agcttgtacg taaaaaaaag atattgcaaa ctagtgtggc
      481 tattaggaat ataaaggcaa aacgacttaa ataatgtaaa acttttaaac gggcgatttt
      541 ttgatattgg taacccatgt aaagtgggat caaatttgca ccaataacaa cggaagtttg
      601 taaaaatgta tagatgtaga atcgaccagg gtttgttgca tatgtaggaa tagtgactgc
      661 tgaaatcaat atacgctgta aaaccatcat tatacaagcg acaaagattg tcaggaagcc
      721 ataactctcc ttctttattt ttttcaagaa taaacacctc acaaaaaaat tggacaaaaa
      781 cataagaaaa cgtttcctga taagttgccc attaataaca tcctaactta gaatatctca
      841 aaaaacaacg tgtagacaga aaaagtacaa aattagcaag aaatggttgt ttgtattgtt
      901 agaattttta gaaaattata cctttagttt ctttgcataa aatgatacaa tgattgttaa
      961 aattgtgaaa gggtggtcat ttgttagaga agtttttgga tatgtcgtta gttcaacgta
     1021 ttgttgttgg tattttagtc ggaatattgt ttggtatctt tttccccaca tggacattta
     1081 tttcaatatt gggaacgttg tttgtcagtt gcttgaaggc aatcgcacct attctggttt
     1141 ttttcttgac gatggcgtca attgccaagc ataaaattgg gaataagaca ttcgtgaagc
     1201 ctattttgat cttatattta gtgggaacat tgctttctgc acttgtagca gttattgtaa
     1261 gttcaatttt tactgttcct attactttac aagaaacagt atctgaaaaa gcaccgcaaa
     1321 gtttggaaag tgttttatct actatgttga cgaatgtcac acaaaatcct gttcaatctc
     1381 ttattgatgc gaattacctt ggcgtcttat tctgggctgt attgttggga ctagcttttc
     1441 gagcacgttc agaatcgaca aaagagttgg tggatcagat atctatagcg ttgtcacaag
     1501 tcgtacaagt gattattgca tttgctccga tagggatttt aggattggtt tatcaatcaa
     1561 ttgctacgac tggaatagct ggattggcag agtatctgca gcttttactt gttttgatcg
     1621 gtacgatgct gtttgtcgca ttagttgtgt acccattcct cacgtttttg tttattaaag
     1681 aaaatcccta tccattgatt ttcttttgtt tgaaagaaag cgcacttccc gctttcttta
     1741 cccgaagttc tgccgcaaat atcccgataa acatgatgtt ggctgaaaga ttgaaattga
     1801 caaaagagtc ttattccata tcgatcccgt taggtgctac gatcaatatg ggcggtgccg
     1861 cgattaccat ttccttgatg acgttgactg ctgtacattc tttagggatt gaagcacctt
     1921 ttgttttgaa agtcctttta tgtattttgt ctgctttagc tgcttgtgga gcttcaggtg
     1981 ttgcgggcgg ttcactgtta ttgattccat tagcatgtag tttgtttggt atctctaatg
     2041 atgtcgcgat gcaaatcgtc ggtattggat ttattatcgg tgttatccag gattcaatgg
     2101 aaactgcctt aaattcgtca agtgatctgt tgtttactgc aataggagag ctaggcgcgc
     2161 gtaaaagaag tggggaaaaa atcaaattga aagattgttt agatggatta agtgaacgtt
     2221 gaacgttaga tatttagtat ttttaaaatg attcgagttt ttaatgagtt atatagatgt
     2281 tcgtgtaaat agaggtttct gttaaatcaa gataaagtcg cttataataa caattaaaag
     2341 cataggtaat tgttattgga ggcgtatcat ggatttttta cgtaaatttg gttttttctt
     2401 tttattatta ggcgtgctga gtgatttttt gactccgtac gtattaggat tgtattatcc
     2461 tgaattagat caaatgaaga gcgtgatcag tgtatttgga gaggtttcca gtcctgtaag
     2521 aaaagcgttt cttatttggt ctgtggtttc agggatcttt ttcatcctgt cattgcctgc
     2581 attgtatgct gcatttgtaa gtacatctcg tgtgttagct accctagtta cagcagccat
     2641 cgcgttctat ggtataggtg attgtatctt tacaggtttg ttcagtatca atacccatga
     2701 atcttcgtgg aatgtctcga catgggtcca taatatcgga tcaggtttag gttacagtgg
     2761 ttttttattg tttccagttc ttcttttctt gatctatcga aaaaatgggc aaatggacgt
     2821 aagccactta taccttggac tgtctgtgat cagtttgctt tttgcggcga tttatggact
     2881 ggcaagaatt ccttctataa attcttggcc attactgaat aaaataggtt tttgccaaag
     2941 aatcagtttt ttctttaatt atgtgcccat tgcctggttg agtatcttgc atctcaaaca
     3001 gtaaaacttg agccgtcaaa ttgaacagtc agcggatact tcatccggtg gctgtttttt
     3061 gtttttctga aatatatatt tccacagttt tcttgtatag cttctgtttt agagaagaaa
     3121 ggagctatat tttgtgctac aatcttaaaa aggtagagag aagaccaaat ggaagacaga
     3181 ggagccacta aaatatgaga aacatcaagt tgacaatcga atatgacgga aaaagatatt
     3241 caggatggca aagacttgga gatgacgata aaaccattca aggaaaaatc gaaaaagtat
     3301 tgcatcagat gacaaatgaa gagatagaaa ttattggatc aggaagaaca gatgctggca
     3361 cccatgcgcg aggacaagta gcaaatttta aaacaaacac agaaattgga ctggcagaaa
     3421 tgatcgactt tatgaatcgt taccttccgc gggatattgt catcaaaaga atcgaagaaa
     3481 tgcctgagcg cttccattca agatataatg cggtgggcaa aaaatacagc tattatgttt
     3541 ggaacaacgt gattccttca gcatttgagc gaaaccacag cttttatttc cctcaagagt
     3601 tggatatgga caagctgaat gcagcttgcg aaaaattagt gggtacacat gatttcattg
     3661 gtttttctgc attgaaaaaa tcaaaaaaat ccacagtgcg tacgattgat gaattatcta
     3721 tcgaacgaaa gggagaaatg cttcatttta cttttgtcgg agacggattc ttatataaaa
     3781 tggtgcggat tatcatgggg acattactag aaatcggcac aggtgccatg ccgatagaaa
     3841 caatcgatga aatatttgaa agcaaagtgc ggcaacatgc aggagaaacc gtccctgcac
     3901 aaggtctgtt tttggatgaa gtttattatc gataaaaata ctttgataat ggatgaaacg
     3961 catgtttcac agtataaata tcgatgttaa ataatgtatg aagcaaatta aagaaatata
     4021 aaaaaagact ttttagcctg tttatgtgta cagctaaaaa gtctttctta aataaaaatc
     4081 tagtgagaat gttgtacttc tcttctcggt tgtttttgtt tagaaaaagt aaatcgataa
     4141 agtaaaaata aaatcaaagt aacgatagat aaatatcttg aagcaattgc tagttttgtg
     4201 tactgataag taataacgac tttatttata cctggaggaa tagttaattc agttgtgcca
     4261 aacttagata attttgtttg tactagtttg ccattaatag aagccacttg ccctttataa
     4321 cgataaatag gtgtcgtcaa gacagtattt ttattattcg aattatctaa ttcaattgta
     4381 agtttg
//
LOCUS       DACDHL010000151         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDHL010000151 DACDHL010000000
VERSION     DACDHL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.155"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(83..1051)
                     /locus_tag="IV228_002743"
     CDS             complement(83..1051)
                     /locus_tag="IV228_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8718583.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IV228_002744"
     CDS             complement(1498..2904)
                     /locus_tag="IV228_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8718584.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IV228_002745"
     CDS             complement(3126..3488)
                     /locus_tag="IV228_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718585.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYLNESDGLFEVIPNNK
                     SQMSESKFTSMFSDVDLR"
     gene            complement(3492..3800)
                     /locus_tag="IV228_002746"
     CDS             complement(3492..3800)
                     /locus_tag="IV228_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718586.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IV228_002747"
     CDS             complement(3809..4228)
                     /locus_tag="IV228_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718587.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IV228_002748"
     CDS             complement(4221..4361)
                     /locus_tag="IV228_002748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718588.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1232 a          995 c          621 g         1526 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttctcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaatg tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 agtgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaatccac gtcactaaac attgacgtaa atttactctc actcatctga
     3181 cttttgttat tcggtatcac ttcgaataag ccgtcagatt cgtttagata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACDHL010000152         4333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDHL010000152 DACDHL010000000
VERSION     DACDHL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.156"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..630)
                     /gene="clpP"
                     /locus_tag="IV228_002749"
     CDS             complement(37..630)
                     /gene="clpP"
                     /locus_tag="IV228_002749"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8718589.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            810..1337
                     /locus_tag="IV228_002750"
     CDS             810..1337
                     /locus_tag="IV228_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8718590.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            1428..2165
                     /locus_tag="IV228_002751"
     CDS             1428..2165
                     /locus_tag="IV228_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8718591.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2169..3086
                     /gene="miaA"
                     /locus_tag="IV228_002752"
     CDS             2169..3086
                     /gene="miaA"
                     /locus_tag="IV228_002752"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8718592.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3088..4317
                     /gene="hflX"
                     /locus_tag="IV228_002753"
     CDS             3088..4317
                     /gene="hflX"
                     /locus_tag="IV228_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8718593.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1471 a          756 c          961 g         1145 t
ORIGIN      
        1 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
       61 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
      121 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
      181 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
      241 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
      301 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
      361 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
      421 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
      481 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
      541 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
      601 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
      661 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
      721 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
      781 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
      841 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
      901 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
      961 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
     1021 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
     1081 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
     1141 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
     1201 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
     1261 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
     1321 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
     1381 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
     1441 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
     1501 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
     1561 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
     1621 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
     1681 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
     1741 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
     1801 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
     1861 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
     1921 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
     1981 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
     2041 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
     2101 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
     2161 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
     2221 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
     2281 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
     2341 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
     2401 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
     2461 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
     2521 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
     2581 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
     2641 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
     2701 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
     2761 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
     2821 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
     2881 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
     2941 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
     3001 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
     3061 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
     3121 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
     3181 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
     3241 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
     3301 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
     3361 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
     3421 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
     3481 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
     3541 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
     3601 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
     3661 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
     3721 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
     3781 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
     3841 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
     3901 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
     3961 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
     4021 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
     4081 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
     4141 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
     4201 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
     4261 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
     4321 tttatttttt taa
//
LOCUS       DACDHL010000153         4286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDHL010000153 DACDHL010000000
VERSION     DACDHL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.157"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..377)
                     /locus_tag="IV228_002754"
     CDS             complement(<1..377)
                     /locus_tag="IV228_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP8718594.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTR"
     gene            827..1441
                     /locus_tag="IV228_002755"
     CDS             827..1441
                     /locus_tag="IV228_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718595.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1606..1848)
                     /locus_tag="IV228_002756"
                     /pseudo
     CDS             complement(1606..1848)
                     /locus_tag="IV228_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2052..3371)
                     /locus_tag="IV228_002757"
     CDS             complement(2052..3371)
                     /locus_tag="IV228_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP8718596.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(3526..4113)
                     /locus_tag="IV228_002758"
                     /pseudo
     CDS             complement(3526..4113)
                     /locus_tag="IV228_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1370 a          753 c          716 g         1447 t
ORIGIN      
        1 cgggtcactt ttaagattga ttcatcgatc gagtaaatca gtagatcttc atcagccaca
       61 tatcttctga aaatattatt tacctgcata tttcgcttga tatataggtt catacgtggt
      121 ggaacaacgt gtagtgtttt aggaaatggt tgtggtaagt cacgtggtct actcacattt
      181 gtaataccat accgcttttt tgcttcagga gaagaagcta atatcaatcc tgaaccagta
      241 ttatcagctc gactcataac aacaagttct gttgttaatg gatctaaatt tctttctata
      301 cattcgacac ttgcgtaaaa gcttttcata tcgattaaaa agtaatcatt tactggctct
      361 tttgaataat ccatcataaa taacactcca ttctttcaca ttatacaaac atatgttcga
      421 tattgtaaag aagaaatagc taaatacata aaaaaagaag cataggtatg ctatcaccct
      481 gcttcttctt ttgatttgta tgatcaattt attctatcac tttactaatt aatttgaaaa
      541 tctaataaaa actctcactc taaaagagtg agagactgca aattagagta cggacttttt
      601 tggagttccg ttctttaata ttaacaaatg aataaaatcc ttacaacatt tgagaataag
      661 ttatcagtag acacttacaa aaaattctca tactatttcc tccatcatta gtttttgtct
      721 atataaggta taccctaaat agacactggt ataacattga aaaatatggc ttcctcgtgt
      781 atgattaggg tgtattttaa atagttgcag aaagtaggta aaagttttga gagttgcata
      841 catacgtgtc tcatcgatcg atcaaaatga acaacgccag attgaagaaa tgaaaaaatt
      901 cggtgcagag agaattttca tcgagaaaca atctggcgca acgattactc atcgacctgt
      961 tttccaagaa gcattagatt acgtccgaga tcaagatatt tttattgtgg aagctattga
     1021 tcgacttgga agaaattatg acgagatcat cgcttcagtc aattacttga agaagaaaaa
     1081 tgtgaagctg atcattacta gtttaccgat catggctgaa gcaattggca atcctctcct
     1141 ggataaattc attaaggatc tgatcatcca aattttggcc atgatcgctg aacaagaacg
     1201 gactgaatcg aaacgtcgcc aggcacaagg aattaagatc gcaaaagcaa acggcgtcta
     1261 caaagggcgg cccaaattat acagtgcaga cgcaaaagat ccccaacgtc gccttgttta
     1321 caaaagcatc gttgaagatc ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta
     1381 caatgttacg agacaaaccg tgtatagaat aaaaaaagac agcatggtca atgacaaata
     1441 gttgatcatg ctgcttttat tatcttctca tctttaattt cctacatata attactatct
     1501 ataaaattaa gtcttactca gaacagataa agcataaaat tccaacaaac cattttttac
     1561 ttaactaaat aaagagtctg ttaatataat cttcacttat ttacgttaat cccctactgt
     1621 atttgttggt tccttatcta tattttctac aaaatcctta ttatcttgtg atttagcttc
     1681 gtataattct ttgtatgtcg acacattggt tatactaatt cctatagcta gttcttgaga
     1741 ctctactttt ctctctagct cctttgacaa agtgatttga gatttatttg gttccgttgg
     1801 agtcaattct atagattctt cataaaaatc tctatacata aagctatccc tgaataattc
     1861 atagctttaa ttctttggca cattttattg aaatatgtat cactaatatc cctatcaact
     1921 gattttgact aaaaagttac ttcaaacatt cactgttcaa aaatattgag gaataaattt
     1981 tgggtgtaag gcattttgag catacttctc cctggataac ttcccagtaa aaaaatcgtt
     2041 agattgttgg attagatcca ttgactggct cgcctcaggc gagcaaagac cctgtagaat
     2101 tcattctgat acacatgata actagataat ttgagataga ctcgtctacc cgtttggact
     2161 aatttcccag caactttcag aaacttcaat cgaatagaat gaatggtcat tcccttttgg
     2221 acttcttcaa agccaatcgt tcttaagaag ttgactaagt tgtaagctat caagctgagc
     2281 atcattcgga catgattctc caaaaagcga ggactgtctg ttttgtcaaa gtaaaagcca
     2341 gctttcgctt ctttaatgaa gttctccatt gtgccacgtt tggcatagat agaaaagatg
     2401 gtatcaggag aaacattttc tgatagattc gtcacgataa attcatgtcg aaagagtagt
     2461 tcgcccgctt cacgtgttga gcgtatacac acgcgacgac tttgtgacca ggattgtgct
     2521 tgataagtgg tcgagaagta ctgaacttct cgttcttccc acttttgatt atcgccataa
     2581 agtactgatt tctcagctat ttgacctagt ctacgattat tcttcagtcg aataacataa
     2641 tgactctttt ttgattcaca cgaatcatac acaccaggtg tagcgaaccc gctgtctcca
     2701 cgaaccaaga tgtcagtgtt tggtagagag tgattatagt gctctaataa aggtgtgaga
     2761 aactccttta cgccttttga tgtatattga tttcctgaac gtagttcagc ttttaagaag
     2821 tcaccagtca atccgtcaaa agctaccaat ggatggtaac cataggtttg gtagtgtgta
     2881 ttataattcg tttgttcttg gtgaccaaat gtatctgaat gggtcgaatc taaatcaatg
     2941 attaattccg tatcattacg gatgaggcgt gctttatcga taagttcttg gttcaaagct
     3001 tgaagttcat ggatattctc ctcagatagt cgatctaaaa atcgggaaag tgaagattga
     3061 gaagcgagtt ctttcctatc taaaacagct ttaaacacag gatcttgtct caggagatta
     3121 gctgcagaat cagctgagta accagcaatt aattgcataa tgaactgctc aagtatggat
     3181 aagttgtcat gagtaaaata cgctcgttcg tcttcaatgt gaatgtgttg cttagccagt
     3241 tcagaaaaac caaagatgtt catcagctct ttcactagga cgagacccga atcactcgat
     3301 aattgaccac ctgtatgaga aatagtgata tttgaattga attttacttg gtttttgtgt
     3361 aagctagtca tgaaaagaac tcctttcttt tggttggttt agtcacttta accatagcag
     3421 aaaggggttc ttttttcatc acttaacggg tgaagatgaa aaagtaattg taagctgccg
     3481 tgaggcagtt tcacatggtt ttaattaaaa gtatgaatta ttcaggctat cattaatttt
     3541 ataaaaaaaa ttaaatgttt ttagcactgc catataacat tcaaaacatt cttctatagt
     3601 tggtttactt ccttgaggag aatgaaccaa tttattacgt ttttctatga aatttttaat
     3661 ttcctttctt atttctttcg gaaactcaat attaaagtac cctttaaaca tgctccctaa
     3721 taatttgtct acagggggag attgtaaatt ctcaaatatt agagttaaat caggtttctc
     3781 ttgttgataa aattccttta cccctatttc acaggctgta attatcgaaa taaacctatc
     3841 tctagtattt gatatatttt tacttctttt aaggagtata agagaaaagg ggatactttc
     3901 gtgtccatgc atgtaattaa ctaattgttg agcttcagaa gttatatctc taattatata
     3961 gttaacggaa ttaggaagtg tctttaactc taatgaaatt tctatccaat tactatcttc
     4021 taatctgtat atgctcgaag tataatttga ttcgtcagca agcatcgcta catcccaaac
     4081 actgatcata aaatctttta aacgttcaat catttcagca atattgctaa aatcagctaa
     4141 tttttcttta gaataaaaaa aaacatttat atcagaatca ataaattcta tttttttatt
     4201 actatatcgt catcattatc ttgaccattt atgtttattg ttccctgagt ggggagtact
     4261 tctttcaaat tcaagttaaa tttata
//
LOCUS       DACDHL010000154         4181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDHL010000154 DACDHL010000000
VERSION     DACDHL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.158"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            65..412
                     /locus_tag="IV228_002759"
     CDS             65..412
                     /locus_tag="IV228_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718597.1"
                     /translation="MDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFS
                     YAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIF
                     LSFIVWVFYLVKK"
     gene            507..1391
                     /locus_tag="IV228_002760"
     CDS             507..1391
                     /locus_tag="IV228_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8718598.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1613..>4181
                     /locus_tag="IV228_002761"
     CDS             1613..>4181
                     /locus_tag="IV228_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718599.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
BASE COUNT         1364 a          708 c          947 g         1162 t
ORIGIN      
        1 atttctagaa gttattcacg tatacggatt tgtttttctc acaaataaaa aggcaggaag
       61 tacaatggat catttgtata taaaatatat tcatcaattt tccgttttgg tattaggcac
      121 actaattata gcggggattg ttgcatttat tattcaaggt cgtgacatat taacagtaca
      181 tcaagagttt ttcagttatg catttggtat tcctctgatc gctataaggt ctgataacaa
      241 aaagtggaag aaacgctatt acattctctt gctatttttg tgtttttatc tgccacctaa
      301 gatttttact ttattaggta tacaatgcct atcattttct tttatttcct atatcattgt
      361 tttaattttc ctcagtttca tagtatgggt attttatctt gtcaaaaaat aaatgaatgt
      421 ttcatgaaca gtacttcatc acagcctaat ttttttcttt atgctatcgt tatattaaag
      481 aaatcaatca gaagggaaag aaagtgatga aaaaagtttt attagtggta aaccccagtt
      541 caggcggtga acaagcaaag gaatttgagc aattagcgat agcaaaattg gaatcggtgt
      601 ttgatgaagt ggtcgtcttg catacaaaaa aagcagggga tgcaaaaaac tttactcgcg
      661 aagcggctac ggaagggtat catagtgtgt ttgtcatggg cggagatgga acagtcaacg
      721 aaggaatcag tggaatcgct gaacaagaac atcggccaaa tttcggattt ttcccattgg
      781 gtacggtgaa tgacttggct cgagcattag ggataccgct agaacctgag gaagcgatca
      841 atcatttttc gattgaatca gtcaagccat tggatattgg aaaaataaat gacgactatt
      901 ttatgaatgt cgtcgcaatt ggctctatcc ctgaagcaat caatgatgta gatgcggaga
      961 agaagacgaa atttggaaaa ctagcttatt tcatgtcagg aatcaaacag ttagcttcca
     1021 cacaatccta ctcgtttcac gtagaagttg atggaaagaa ggaagaaatt gagagcagta
     1081 tcttgctgat aggactaact aactcagtag gcggttttga aacattactt ccaaatgcaa
     1141 aagtggatga tggtaagctc catttggttt atttaaaaga ttcttctttg atcgatacgc
     1201 ttaaaacatt gccagaccta ttaaaaggag tagatgaatc tacgaaaaat ctagcctatc
     1261 ttacttgtga gcaattagtt gtgtcattag ctgatgagga agaattgacg acgaatgttg
     1321 atggagatga aggggataaa ttacctgtga caatcaaagt gttaccctca catttacagg
     1381 tttattgtta aaatggaggt tgtgaccgaa gagttgtcag gaggacatcg gttgttcgga
     1441 ataagggact gtgacaagac ttttgtcaca gccttttttt gtacaaaaaa gaaattttca
     1501 cctaacagaa aaatttgcca taaaaataca tattttaaat atgaaagtac aattacaaat
     1561 aaattaaaac gttaaaataa aattgtcaaa acgtcagaga ggatggtgga gaatgaatca
     1621 atatgtaaaa aagctgattg cgtcgatttt gtttgtcttc cctctcttga tggtgacatc
     1681 cgtttttgcc gatgtagaga aaacaagcga atcaacgcaa tcagagaatc aaagttttga
     1741 caagactact ttttcagaag aatcaattac ttctgaaact acagaatccc aaagtgaggc
     1801 gcctacttct aatacaaggc aagaagaaaa taagacaagt gagacctcag aagtagctga
     1861 acaaacgtct gatagcagtg aagaggtaca ggttagagtt gtttcagcgg atgggtacag
     1921 cgaagctgcc acaacagaag aactgacgca gcttctcgca gacgaatcag tagctaagat
     1981 tcgtttaact cagcctttga ctttagacag ggaattggag atcaagcgag atatcgttat
     2041 cgattttggc ggacttgctc ataatttcgg cacacatcac atctatatca atgagacacc
     2101 tcagtcgatc gagtttcaga attttaaagg aacggcagct catccaggtg ggttgatacc
     2161 aaatatcgat ggaaatgcca ttatcattgc ttatatgtcc gatttttggg gaaacagata
     2221 tcattttacg ggagaaataa aatttaccgg gacacttgac ttatatgatg gttctaaatt
     2281 aggcctgatt tacgcccctc gtgcaactgt cacattagat ggtgtttccg gtgtattgga
     2341 tgtccaacca gtgaaagaag ggatgaatgc cgctccgggt aaagcttatt tttgtagaag
     2401 ctatcagcta aatgtaatca atggtagtca gctttatgga ccatatctag gaaaattcta
     2461 tggttttcta gattcaggtg atgagacggg cagtggaaaa acagcaccag gactccatat
     2521 cttgagtggt tcaaaggtct ctcttgacta tgatcgggca gatggcgtca gcgatgaagg
     2581 agaggctgtc gatacgctac ccgggaatgt cacatttaaa gtcagtggct caggaagtga
     2641 attttcggta aatacaaaga tcaatattac caacgacaac aatcgcggaa ttatccaaat
     2701 gagaggctca ggatcaagag tcgctgtgtc gaatgatggg aaaatcacaa tcaatacagc
     2761 gactactggc ggatttcgcc tgcaaggaga ttctagtcaa atccatgtat caagtggtgg
     2821 acaaatctcg gtcaaaatgg ctggagatgg cgaccaacct ggaaacaatg ggatgcgttt
     2881 tgtagggaaa ggactttctt tgatcgtaga aggagccgga tcagtcatcg atattgataa
     2941 gcaaagtggt cgttctagcg cagttcgttt tgagaacggt acgcaaaaac taacagtaac
     3001 taatggaggc tcattgaagg taagaaatgc aggggatggc aaatcttatg tgaacagact
     3061 caatcaagga aaccaagcga tccaatttta tgacgcttct ggttttctag aaagtccagg
     3121 gtcagctgat tttacggtta ctggagaaaa atcaaatatc gatatacgcg cagattccgg
     3181 agctaccgta gatacaactg gagatattga tttgaacttt atggctggcg aaaaaactta
     3241 tttagtcatg gtcgggaaaa ctggggaaga taagactggc atcatcagtg gggatgtgtt
     3301 gaatgtagac ttagaatctc ctacctattt cgatttccaa aacaaacgga tcggaagcga
     3361 gactaatggc ataggtggct gggtatttca aggaaatggc gattcttctt tgaagatcgc
     3421 tcattcagaa attgctttat ggcgaaaggc aggagtaacg ttcgataact ttgaaaaaga
     3481 tccggattat ttttctgata tgacagactt atcgatcaat ggaaccgact taggtaattt
     3541 tgtatcaagc cctagtgcag gtttaacaaa tgaatttgct cagacaggtg ccggaatgaa
     3601 aaattataat cgaatcagtg ccaataacca gcttccgatg attgaatcct taagagtacc
     3661 aacgaatgca gataaaaaaa tctatgggca tgtcgttgtt cccgaaggtg tcgaggcaaa
     3721 gccaagagat gcttggacag gcgaagtaga agtgacttta cgtattactt atgcagatac
     3781 gacgaaatca cctgtagaaa ttactgctct cacacaagga caaacagatg aaaatgaagg
     3841 gacaggatat aatccgtggg gagaaggcga acaaggcggt ttgtttgagg ttcaagtacc
     3901 caacgaagaa agactaacaa cgggggatca agtgcaaata gttgctgcta aaaaagtaag
     3961 aggcggccaa gaagtgacag aactggactc tgccaaaacg acagtcgatg tgattcctcc
     4021 tgagcctgct aaattaagta ccacattgat caatgcagct tctcgctcaa tcacaggtac
     4081 aggcgaagca ggagctttgg tttcattgaa aaaaaatcaa gctgttttgt ccgaaacaac
     4141 tattgatgat acaggtaaat ttgttctttc tatcccagaa a
//
LOCUS       DACDHL010000155         4114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDHL010000155 DACDHL010000000
VERSION     DACDHL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.159"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1014..1304)
                     /locus_tag="IV228_002762"
     CDS             complement(1014..1304)
                     /locus_tag="IV228_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718600.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1646..1942
                     /locus_tag="IV228_002763"
     CDS             1646..1942
                     /locus_tag="IV228_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718601.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="IV228_002764"
     CDS             1949..3901
                     /locus_tag="IV228_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8718602.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..>4114)
                     /locus_tag="IV228_002765"
     CDS             complement(3973..>4114)
                     /locus_tag="IV228_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8718603.1"
                     /translation="EFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYERKNL
                     LS"
BASE COUNT         1424 a          705 c          707 g         1278 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttca
//
LOCUS       DACDHL010000156         4065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDHL010000156 DACDHL010000000
VERSION     DACDHL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.160"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2759)
                     /gene="brxC"
                     /locus_tag="IV228_002766"
     CDS             complement(<1..2759)
                     /gene="brxC"
                     /locus_tag="IV228_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HAP8718604.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQ"
     gene            complement(2784..3359)
                     /locus_tag="IV228_002767"
     CDS             complement(2784..3359)
                     /locus_tag="IV228_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HAP8718605.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3359..3958)
                     /locus_tag="IV228_002768"
     CDS             complement(3359..3958)
                     /locus_tag="IV228_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAP8718606.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1214 a          780 c          548 g         1523 t
ORIGIN      
        1 aattgacctt ttaaatattt cttgaaattt tccattgtat gttcattgtt attttcatca
       61 acaatattac gactttgaaa cagttctcta ccgatttcgt caaccaattt ttttgctttt
      121 ggcgaaatca ccgcgcttgg ttcaattttt atcttttcac tttctcgtcg tttttgaata
      181 atcattacaa tactatttgg atcatccatc cgtgaaattt gtacattgtt ataaagtaaa
      241 tcaattttac cagacatgaa aagtttagcg atgcaccatt ccacatcaag ttctgtgaat
      301 ccatacggtt gttttgtttc aaaacgatca cgaattgttt tcatcgtaat cgttaggtga
      361 tttctctttt gatcattgat aaaattgaaa atttcctgaa ctgcttgctc atttgatgga
      421 tcttcaataa tttcattttt gctttctttc actaaatata agatatcttc tttttcttta
      481 ggcgcatcaa tttggtttaa tttatggtat acttcttcag ccattttctg aagtgctgat
      541 tggaattttg ctttaaagtc ggagcctgtc agttgaattt ctgcaccgtt atagaaaaat
      601 tttgcagatt tcaacagttc atttaattga tcgttgatac ttgcacttag ttcctgtaat
      661 tcaccagatt tttccgaacg aatcgcgtca aagttcttta ctacactcga ataagtcgtt
      721 gttaaataaa gattcattct agcatacatt gctatatcat ctttaaattc tttatctgat
      781 ggcaattcta cgaataataa gttacgggaa ctcttaagcg caagttctgc ctcttgacct
      841 gctaaatcag agtatggtga aataaaatta actgtcattt cagcatttcc agcttgcttg
      901 tatggacgtt gatctaacac ttgtgcataa gtaaacgtat accggttttt aaatttattt
      961 ccacctgaca tgcctaattt ttcataggca aaactatttc ggtctagaac gccgtcaaaa
     1021 attcgtttag atagttctac gatcaaatca ctatctgtaa tattttgatt tttaatctca
     1081 cgagtaattt cctgttcttc atcagtttgg aaaatataac tatctaagtt cttccgcaca
     1141 aagttttcct taattaaaag gtccaatgct tcttctactc gttttttagt ttccaggcgg
     1201 tcttcgttga tatttcttat catcaagctc gtaatatttt caacatttgg tttgatggct
     1261 tgatcaacat atttaatcat gaataaaatt cgtaagacat ttacgttaaa gttatctata
     1321 tcagttggtc tttgaatatg agaattattt gtagcttgtg taatcactcg gctaattgct
     1381 gtatcaatcc aacgttctaa cgagtaataa aactgatcta aagaaaccaa actggttgta
     1441 tcattttcct tatttcgttc agcggcttct ttaaacattg ctagtaatga acgttcacca
     1501 gacgataagt ttgatccttg atagctgtga ttacgaatct cggttagaat ttgccccaat
     1561 aaattgaatt gatagggaat aaatggataa acttctgcgt aattttcagc atcttggaag
     1621 agctttaatt caggactatc aacaaagagt aatttatttt tcaattctgt ttgaacagtt
     1681 tcaaattgta aggctaaaga atcattggca aactcttttt tcttcaaaat acgttcttta
     1741 attacttcat cggcatttgc tgaagataaa ctcacagttg tggcaaaacg atccttgatc
     1801 ttagaaaaat cattatcaga attgttatca aagtttgtta cgtgatcaat cgcttcctga
     1861 cttgtcacta atacccaagc cttaccttca caaattcgac ccaaatcttc cacaacggtt
     1921 tgtaactgca aagtcaactc acgattaccg ccgatatatt gtccgacctc atcgactaga
     1981 aaggcaatat gatggtctgc ttctttggaa tcaagatagc ttttaactag atttgcaaag
     2041 tcatcaatgc ttattaaata atttcctgat gctttttcta aaaaattatc aatagcggct
     2101 tcactcataa aatcgatttt aagtgccgct tctttgatat catcttggat ccaatcaaac
     2161 tcatggcgct cttcttgcca ttttgcccca ttaatctctt caaaagcagc tttgaatgca
     2221 tcaaactttt cttctttaat taaccgacgt tcaaaatctg ctagatgtgg aatcgtgctt
     2281 aatccttgca tttcgttaaa tacctttaaa aaaacattca cgattccgtt tttgtctttt
     2341 ttaccacctg catcactttt tgaatcaata ttaaacagaa taacatctgt atttatttgt
     2401 gagcaccgtt ccatcgttgc caaagtcagt ggatctttga ttttcccatc ttctttgaag
     2461 taatcaatgg ctttcttgtc atttatttct cgatttgcga gcaagtaaga aataattttt
     2521 aagaaatgag atttacctga accaaagaac ccctgaatcc aaacacccat tttatctgtt
     2581 ggtgtattga tactccgttg gtaggcatca aaaattgttt taaagtgttt ttgtagttct
     2641 ttcgtaacca cgtattcttc taattcttgt tgaacatttt catcctttgc ttgaccgacc
     2701 ttaataactc cttgaatatt tcggtcaatt ggttttgcaa aaatatcttt aattagcatt
     2761 taatctccta ccttccatcc caactaaatt ctaaatgctc gataataatt atcatcttta
     2821 ttggtattaa gaattgtcaa acttaattca tcatattccc ctggatacat catcacaact
     2881 ggtacgcgat caaatacctg atgcatttta tttaaaatag agtgcgctct aattaatgga
     2941 tataattcgc caattcctgt caggaacaca actgattttt ctggtgtatg ggcatccaca
     3001 tattcaaaaa atacattttc ttcctcaggt aaatccattt tcaataaacg accaatgctt
     3061 tccactaatt tttgtaaacc acgtcgttct tccatcttaa tcgctttttc agtgaaaccg
     3121 cgtttttcta gatactctaa cataatcaca tagagattat aaactttcac ttcataatta
     3181 tcaccaacag atgttccaga tatatcatta atataatctc ttacaattaa ctcgtctttc
     3241 ggatcgtagt caaagatata atagcctact tcattaccta aaccacgatt caataaaaaa
     3301 tcttcttttt gaattacttc attcaacttt tccaaatgct cttgaatcga taatgacatt
     3361 ataaaattcc tcccagtgct agtacaatat ctttacgatt cttgtcaact aaccattgaa
     3421 tcaatcgttt atctaataat atcggagtca ttcgtccata ttctttttcc cctctgacta
     3481 atcctgtttc aattagattc gtttgataag cggcaataag ccgtttaatc gtataatctt
     3541 tccattttga aatatcttct gaacttctcc gcttctctgt aaaaaaagaa cggtaatcaa
     3601 ctggtgttaa ttgcaaattt cctttacgta gattttcagc atatacctca gtcataaatt
     3661 cattaaaaag atcatcaatc ataaggatgg aaatcaaatt cactaacttt tgattatcta
     3721 tatttaactg attgaaaagt tgcaatccag cttcatccag ttgactaact ctccttttca
     3781 cgttctgata gatttctttt tgacggttac tgctcactgc aaaaaaaata ttttcatgtt
     3841 catttttctc tttaatttct atatctgtat atccttctag tcgtaattga atatataact
     3901 tgaactctga aaaccaaaag cgtttagaaa ctagaccagc agaatatttt ttattcaata
     3961 gattgcctcc taatcatcta taattcaata agtaaacaag tgttttttgc tcatcgttag
     4021 cattcatttt ttagtatcta ctatacaata catatttatt atagt
//
LOCUS       DACDHL010000157         4060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDHL010000157 DACDHL010000000
VERSION     DACDHL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.161"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            85..1434
                     /locus_tag="IV228_002769"
     CDS             85..1434
                     /locus_tag="IV228_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8718607.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="IV228_002770"
     CDS             complement(1562..2152)
                     /locus_tag="IV228_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8718608.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="IV228_002771"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="IV228_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8718609.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1168 a          743 c          788 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc
//
LOCUS       DACDHL010000158         3911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDHL010000158 DACDHL010000000
VERSION     DACDHL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.162"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..937)
                     /locus_tag="IV228_002772"
     CDS             complement(<1..937)
                     /locus_tag="IV228_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8718610.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIID"
     gene            1315..2706
                     /locus_tag="IV228_002773"
     CDS             1315..2706
                     /locus_tag="IV228_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8718611.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            2722..3762
                     /locus_tag="IV228_002774"
     CDS             2722..3762
                     /locus_tag="IV228_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8718612.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            3781..>3911
                     /locus_tag="IV228_002775"
     CDS             3781..>3911
                     /locus_tag="IV228_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8718613.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYA"
BASE COUNT         1301 a          641 c          686 g         1283 t
ORIGIN      
        1 tatcaattat catgggttta aaataagaag tgatggagat aaccagattg ttcttagcgt
       61 caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc tcagaatcta
      121 atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca ctggttctag
      181 gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta tacatccctt
      241 tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac aaaccatatc
      301 ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga gacttatcca
      361 ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg tttggtatcc
      421 ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta cttgtctgat
      481 acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg ccggttatac
      541 cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt gaacagactt
      601 ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag tgcttgattg
      661 taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa taatctaata
      721 ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc agcaaagata
      781 caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa ccattccatt
      841 catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata tttggttgtc
      901 gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc actttcttct
      961 ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg taatacaggt
     1021 attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata taaaggttca
     1081 ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta ccaacattag
     1141 tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt agaggtgtat
     1201 aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac taggttttta
     1261 aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt atatatgaat
     1321 aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga tgttattgta
     1381 tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat cccattacga
     1441 aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat tgcgttgaac
     1501 attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt gtttgtcaca
     1561 gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc gggccgctgg
     1621 tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct tttcttaagt
     1681 atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa aaaggtcgtc
     1741 attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc aaagaaaaac
     1801 aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat caaaaatatg
     1861 attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga aaaaatcaaa
     1921 atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac atttgaaaac
     1981 ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat tgaagcatct
     2041 ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat catcgtttca
     2101 cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt agtcaaagtg
     2161 acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca aaaggaattt
     2221 tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg accagtttta
     2281 gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc tgtccgcttt
     2341 gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt aggaccacgt
     2401 cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta ctacttacgg
     2461 cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata tgtctctgat
     2521 tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt gatgcttgat
     2581 ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc tcaaggactt
     2641 gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga aaaaatcttt
     2701 cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt accagtatac
     2761 aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga ttatgaagag
     2821 tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat cattcaagaa
     2881 ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg gcccggatta
     2941 gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt cgatgcagat
     3001 gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa gagtccaaaa
     3061 gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag aatcgtttcc
     3121 gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt tataaaagac
     3181 atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg ctatcgtagc
     3241 gatattctga aaaataacca aattctattt aaaacttata atagttgtga ggatcgaata
     3301 tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa agtagaatat
     3361 atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa caagttcgaa
     3421 acatttaaag aattctattc attagcaaat actgtaacag acgatattaa taaagacggt
     3481 atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta tagtacctct
     3541 gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga tccagtgatc
     3601 cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac aaagttgctt
     3661 tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt cgttgaagtg
     3721 aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga ggaaaatata
     3781 atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga gtctttcatg
     3841 atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt gatttatgaa
     3901 gattatgcag a
//
LOCUS       DACDHL010000159         3903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDHL010000159 DACDHL010000000
VERSION     DACDHL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.163"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..561)
                     /locus_tag="IV228_002776"
     CDS             complement(<1..561)
                     /locus_tag="IV228_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8718614.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLL"
     gene            complement(563..1231)
                     /locus_tag="IV228_002777"
     CDS             complement(563..1231)
                     /locus_tag="IV228_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8718615.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1362..1946)
                     /locus_tag="IV228_002778"
     CDS             complement(1362..1946)
                     /locus_tag="IV228_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8718616.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2281..2511)
                     /locus_tag="IV228_002779"
     CDS             complement(2281..2511)
                     /locus_tag="IV228_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8718617.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2526..3476)
                     /locus_tag="IV228_002780"
     CDS             complement(2526..3476)
                     /locus_tag="IV228_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8718618.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3473..>3903)
                     /locus_tag="IV228_002781"
     CDS             complement(3473..>3903)
                     /locus_tag="IV228_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8718619.1"
                     /translation="TIGVSVLVLVTVVLFFKELLITSFDPLMAKAMGMNVSFYHYLLM
                     ILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGACSSLLGLFI
                     GYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERKL"
BASE COUNT         1312 a          694 c          505 g         1392 t
ORIGIN      
        1 aagtaagtca gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg gttcatccaa
       61 tataattaat ttagagttta gcgataaact acatccaata gaaattttct gccgttcacc
      121 acttgatagc gaaaaaatgt ttttatctaa tatatttgaa agatctaata attcagctaa
      181 ttcattgatt cttttatcca tatgctcttt tgtaaatcca aagttttcca tcgaaaatac
      241 taactcactc gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac ttacgtagcc
      301 aatatttttt gatatacttt cttgtgttaa ggattgtagc gagttatttt caaataaaat
      361 ttccccaacc aattttccgt cataaagtct tggaataagt ccattcatta actttaatag
      421 tgtggatttc ccactcccag aagaaccagt aatcaatgta agactacctt tttttattga
      481 aaaattaagg tcaacaagcg cttgttcctt aacatcctta tatgaaaaag aaacattgtt
      541 aaattcaaac aattctttca cctcaaatca aaatacttaa gaaaaaaata gtaacggaaa
      601 acaggatgat atagtctaat aagctcatgt ttgttttata gtaagaactt cttttcacag
      661 gagattctat cccctttgtt attgcattag cggtcatttc ttcagcaatc atagttgcac
      721 gtataagaac gggaactaac agaaattcca aatattgcag aggctgaaaa aaagacaagc
      781 gaatgtttct catttttaaa gattctttaa tcattcttat ttcagaacga taagtcggaa
      841 agaatctgat cactactgta atacttaaca ttattttttt cggtaaatgt attttttcta
      901 aagaggataa taattcattg ctagtataga cactaaccaa caaccatgag atcattacga
      961 ccggcaacat tcgaaataca atatataagg atgtcccaaa aaatatagta aaaaactcta
     1021 tcttgatagc atcaataatt ttcattacta aaaacagaac tgaataaatc aagatgaaat
     1081 aaatacatgt ctttatttta cgaaacatca atactaaaac tgtagcaata atgaaacacc
     1141 ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag
     1201 taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac
     1261 tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa
     1321 agcataactg agaacaattc tcaatgcaag ctttttttta attaactcag taattcaatc
     1381 cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt ttcttttgaa
     1441 tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta gaagtggatt
     1501 tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa cttatctaga
     1561 caacagactg tcttttttgt cacaaatgat gtaatttttt caacaatatc ttgatatttg
     1621 gttccagctg attggaataa aaccaaatcg aatatacgtt tattctcaaa aatataatta
     1681 ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc tatattttct
     1741 aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc attaatatgc
     1801 ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata aaaagcaccc
     1861 gtagttacgc ctgcggtcaa gcacagttgc cttatcttag tttgctgaaa tcctactttt
     1921 tcaaaatggc ttagtccact ttctattaat ttttggtgag ttgtaatata atctctggtt
     1981 tctatgggta cttcctccta ataagatgaa ttaactatat tatcttacac aggacttgaa
     2041 gtttcaatcg ttaacgtcta ttactgaact cattccttag agctaaacta atgagaaaga
     2101 gcaaaataac tgatacaaaa agcaatccta caagaattaa tagactcatc taaatcattc
     2161 accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta ttaggataag
     2221 tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa agccttttac
     2281 ttaaaacctt tttttgtaat cgtcaataaa ttttttccct ttatcagtta attgaaatcc
     2341 tatatctaaa tagtcctcat atctaagttt agtcagcatc tcacttacag atgctggact
     2401 aacacttaga acttttgcta aatcattatt ttttaatttc ttttttcttt ttgataattc
     2461 ctgaattgct agtaaataaa ttagtatagt agttgtttta atttgtacca tacctaccct
     2521 ccaaattatt tcgaaaggcc ttcagcaatt ttatccaggt tccatttcat catcgcatag
     2581 taactatcac catcttgtcc tttttctgca attgaatcag taaaaatcgt tgaatagata
     2641 ggaatattgg tatcttttga tactgttttc attggtctat cgtccacact gctttctacg
     2701 aataaggagg gaacttttgt tgtgcgtaat ttttcaacta agtgttttat ttgatctggt
     2761 gttccttctt cttcagtgtt gatttcccaa atgtaagcag aaggcacatt atacgctttc
     2821 gagaaatatt taaagcatcc ttcacttgta acaatcattt ttttatcatt cggaattgaa
     2881 gcaaatttag atttagcttc tttgtctaga gaatccaatt tttcaatata cttatctaga
     2941 ttttctttat agaatttttt attatcagga tctttttccg ctaactgttt ttcaatattt
     3001 ttagcgtaaa taataccgtt ttctaaattt aaccaagcat ggggatcttc cttccctttc
     3061 tcactctgac cctctaagta aataacatct atgccatcac ttgctgcaaa atagtctttg
     3121 ttttcctctt tgttcgcatt ttttactaat tttgtaaacc aagcatttcc tccagtttcc
     3181 aagttaacac cgttataaaa aatcaaatct gcttttgaag ttttttgaac atcttcaggc
     3241 aaaggttcat attcgtgggg atctttccca ataggtacga tactgtgtaa atcaatttta
     3301 tcttttgcta tattttcagt aatatctgct aaaatcgaat tagtaactac tacctttaat
     3361 ttttcgttcg aagccactgt atctttgtct ttactatttg ttgaacatgc cccgaatata
     3421 aaaagagcaa tcaaaatccc cagacttccc agtactgctt tccattttct catcataact
     3481 ttctctcctt ctttcttaaa aatttttgtt ttggtgagat tagaaaacta attacaaata
     3541 agaatgccgc tgttaaaaca atacttgatc ctgctgctat gttaaaacta taaccaatga
     3601 acaaacctag caatgatgag cacgctccga ctgtcgccga aataaatatc atagttttta
     3661 aactgtttgc atataaatat gctgtagcag ctggtgtaat taacattgca acaattaata
     3721 tagttcccac actttgcata gctgttactg ctactaaagt taatagaatc attaatagat
     3781 aatgataaaa actgacattc atccccattg cttttgccat taatggatca aaagaagtga
     3841 ttaataattc tttaaaaaat agaacaactg taaccaacac taaaacagaa acgccaattg
     3901 taa
//
LOCUS       DACDHL010000160         3849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDHL010000160 DACDHL010000000
VERSION     DACDHL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.164"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1154)
                     /locus_tag="IV228_002782"
     CDS             complement(<1..1154)
                     /locus_tag="IV228_002782"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8718620.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1339..3780
                     /locus_tag="IV228_002783"
     CDS             1339..3780
                     /locus_tag="IV228_002783"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8718621.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1231 a          721 c          840 g         1057 t
ORIGIN      
        1 tatattttgt agtaacgaat atcattttcg atctttgtaa tcgaaaaagg tacatctgaa
       61 gtatattctg cacattctcc gtgttcagga agcgtctcac tgaaaatcgt ttcatattcg
      121 tcaacagtga tccgtgttcg agaatctagc atctcttgat gagattgtgc gaagagatat
      181 tcttgatagt tttcttccag ataacctgta aaaaattcac ttactgcacc agaaccgtag
      241 ctgaatagac cgatgcgatc tcctggctgt agtgatttcg aattttccag tagagaagtt
      301 agccccaggt ataatgaacc agtgtaaaga ttaccgattc gtcggctgta acggatgctt
      361 tcttcatagc gagccataag acgttcctga ttttcttcgt ctgtttggtc taatacgctt
      421 tgcaatgcct tttttcccat cttagtatac ggaatatgga aagcaatcgc ctgataatct
      481 tcgagtcctc gacccgacaa ttctttatgt cgattccaaa ctttttggaa tgattcgata
      541 tacgtagaat tagataaagg accatcaaca acaggaaatt cgctataatc tggacgccag
      601 aaatcataga tatcttctgt cagaaatacg ctgtcgtctt caatcgataa aatacgcggg
      661 ttttgagtga tcatcatcgc aacagcaccg acaccttgcg tcacttcacc accgcttgcc
      721 aagctgtaac gagcaatatc acttgctatg actagtactt ttcgttctgg atgatttttg
      781 acatattctt tcgccatgtg caaagctgcg gtgccaccgt agcaagcctc ttttacttca
      841 aaagaacgag caaaagattg gattttcagt aaatggtgga taattaccgc actcgctttt
      901 gaatgatcaa tcccggattc tgttccgacg atgaccatgt cgattagctc acggtctttt
      961 tctgttacga ttttgctggc agcatttgct cctagtgtaa tgatgtcttc atttgcacga
     1021 ttgactgcca tttggtcttg accaatccct atatggtatt ttgcaggatc atccccacgg
     1081 ctttctgcca actctgtcat atctaaatat aaattaggaa taaaaaagga aagacgatca
     1141 atccctattt tcatttcaac acctctttac ttatgataaa aaacttactt tactaaggta
     1201 tcataaaaag attaaaagaa atataaaaaa gagaatattt aaggattgtt aaaatttaat
     1261 cttagaaaaa aataactgga acaagagcta tttgttttta cagcaaagaa tggtatattt
     1321 ttgtaggagg tggcttactt gaaagaagtc gttatgatag atgctgcaag aacgccgata
     1381 ggaaagtatc gcggaagttt aagtccattt actgcagttg aattaggcac acttgtgact
     1441 aaaggattat tagataaaac aaaactgaaa aaagataaaa tcgatcaagt aatatttgga
     1501 aatgtcctgc aagctggtaa tggtcaaaat gttgccagac agattgcttt gaacagcggt
     1561 ctccctgtag atgtcccagc aatgacgatc aatgaagttt gcggatctgg tatgaaagca
     1621 gtgatcttag cacgccagct gatccaatta ggtgaagcag aacttgtgat agccggcgga
     1681 acagagagta tgtctcaagc acctatgctg aaacagtatc agtcagaaac aaatgaatat
     1741 ggtgaaccaa tttccagtat ggtcaacgac ggattgactg acgcattttc aaatgcacat
     1801 atgggattaa ccgcagagaa tgttgcaaca caattttctg tgagcagaga agagcaggat
     1861 cgctatgcct tgtcgtccca gttgaaagca gcacatgctg tcgaagccgg tgtattttct
     1921 gaggagatca tcccagtcaa gatttctgat gaagacgtgt tatctgagga tgaagcagtt
     1981 cgtggaaata gtacattgga aaaactgggc acgttacgta cagtattctc agaagaagga
     2041 actgtaacag caggaaatgc ttccccgttg aatgacggtg cctctgtggt gatccttgca
     2101 tccaaagaat acgcagaaaa taataatctg ccttatttag caaccatcaa agaagtagcg
     2161 gaggtcggta ttgacccaag tatcatggga atcgctccaa tcaaagccat tcaaaaattg
     2221 acagatagat caggaatgaa tttatcaaca atcgatcttt ttgagatcaa tgaagcattt
     2281 gcagcttctt ctatcgtagt tagccaagaa ctgcagttag atgaagaaaa ggtcaatata
     2341 tatggaggag caatcgcatt aggccatcca attggtgcaa gcggtgcacg tatcttgact
     2401 acattagctt acggattgct tcgtgaacag aagagatacg gtattgcttc attatgtatt
     2461 ggtggaggtt taggcttggc agtcttgtta gaagcgaata tggagcaaac acataaggat
     2521 gtgcaaaaaa aaaagtttta tcagcttacg ccatctgaac ggcgcagtca attaatagaa
     2581 aaaaatgtat tgacacaaga aactgcgttg atttttcagg aacaaacact ttctgaagag
     2641 ctctccgatc acatgatcga aaaccaagtg agtgaagtgg aaatcccaat ggggatcgcg
     2701 cagaattttc aaatcaatgg caagaaaaaa tggattccga tggcaacaga ggaaccatct
     2761 gtcattgcag ctgcaagtaa tggtgcaaaa atctgtggaa atatctgcgc agaaacacct
     2821 cagcggctga tgcgagggca aatcgttcta tcaggaaaat cggagtatca agcagtgatc
     2881 aatgcagtta atcatcgaaa agaagagctg attctttgtg caaatgaaag ttatccttca
     2941 atcgtgaaac gcgggggcgg tgttcaagat atctctactc gggaattcat ggggagtttc
     3001 catgcatact tatctattga ttttttagtg gatgtaaaag atgcaatggg cgctaacatg
     3061 atcaactcga ttcttgagag tgtggcaaac aagttacggg agtggttccc tgaagaagag
     3121 attctattca gtattttaag taattttgct acagaatcat tggcttctgc ctgttgtgaa
     3181 atcccattcg aacgcttagg gagaaacaaa gaaatcggtg aacaaattgc caaaaaaatc
     3241 caacaggcag gggaatatgc gaagcttgac ccttaccgtg cagcaacgca caacaaaggg
     3301 atcatgaatg gtatagaagc tgtagtggca gctacaggaa atgatacacg tgcggttagc
     3361 gcttcgatcc acgcctacgc ggcaagaaat ggattatacc aaggattaac agactggcag
     3421 atcaaagggg ataaactagt tggaaaacta acggtgcctt tggctgttgc cacagtaggc
     3481 ggagcttcca atatcctgcc aaaagcaaaa gcatctttag ctatgttaga tattgattca
     3541 gccaaggaac tagcacaagt aattgctgca gtagggttag ctcaaaacct ggctgctttg
     3601 cgtgcattag ttacagaagg aatccaaaaa ggccacatgg gacttcaagc tcgttcatta
     3661 gcgatctcaa ttggggctat tggtgaggaa atcgagcaag ttgcgaaaaa attacgtgaa
     3721 gcagaaaaga tgaaccagca gacagctata caaatattgg aaaaaatccg ggaaaaataa
     3781 aaaagcaata tctttagaag aataaagcaa gatttacagt tattttattt actgcagatc
     3841 ttgcttttt
//
LOCUS       DACDHL010000161         3839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDHL010000161 DACDHL010000000
VERSION     DACDHL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.165"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..996
                     /locus_tag="IV228_002784"
     CDS             <1..996
                     /locus_tag="IV228_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8718622.1"
                     /translation="EAVQVSLLPFFEKATSLLQEFSKWWGSLDQDTKNWIITIAGIAA
                     AVGPVLIVLGSLMGSVTKIVGEVKSFIGVWQGLAGLFGMSGGWFALAVIAIGALVTGL
                     IWAYNNVKWFHDGVNSFFKGVSDVAVEIFNFLSGYFGGFFDGVIANFNNFYNAGKRIF
                     SGFLDFITGVFTGNWSKAWQGLVDIFGGIFDGIVAVGKAPINAMIGLINGFIGGLNNI
                     KIPKWVPGVGGKSFSISKLPYLAQGGHLINGQAIVGEAGPELLTAKNGKTTVTPLSDE
                     EKRKGISGRVSGGSIEQHIHIGKVDANNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            1008..1712
                     /locus_tag="IV228_002785"
     CDS             1008..1712
                     /locus_tag="IV228_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8718623.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            1709..>3839
                     /locus_tag="IV228_002786"
     CDS             1709..>3839
                     /locus_tag="IV228_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718624.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT         1268 a          578 c          873 g         1120 t
ORIGIN      
        1 gaagcagtcc aagtaagcct tttgcctttt tttgaaaaag caacttcctt attacaggaa
       61 ttctctaaat ggtgggggtc tctagatcaa gatacaaaga attggatcat aacaattgct
      121 ggtatagcgg cagcagttgg accagtacta atcgttcttg gttcacttat gggatctgtt
      181 acaaaaatcg ttggggaagt taaaagtttt ataggagttt ggcaaggact tgctggttta
      241 tttggaatgt cgggaggctg gtttgcatta gcagttatag ctattggcgc attagttact
      301 ggactaattt gggcatataa taacgttaaa tggtttcatg atggagtgaa ttctttcttc
      361 aaaggtgttt cagatgtagc tgttgaaata tttaattttt taagtggtta ttttggtgga
      421 tttttcgatg gtgttatagc aaatttcaat aatttttata atgctggaaa acgaattttc
      481 agtggttttc tagattttat cactggagta tttacaggga attggtcaaa agcatggcaa
      541 ggtctagttg atatttttgg aggtattttc gatggtattg ttgctgtagg gaaagctcct
      601 attaatgcca tgattggatt gatcaatgga tttattggtg gattgaataa tataaagata
      661 ccaaaatggg ttcctggtgt aggtggaaaa tcattttcta tttcaaaact gccttattta
      721 gcacaaggcg gtcatctaat caatggacaa gcgattgttg gtgaagctgg acctgagtta
      781 ctgactgcca agaatggtaa aaccacagtt actccattat cagacgaaga aaaacgcaaa
      841 gggatcagtg gaagagtttc tggaggtagc attgaacagc atatacacat tggcaaagtc
      901 gatgcaaaca atccatctga gttagatcgt atgaatcgta aatttgctaa agcaaacaaa
      961 caggctattc gtgatttggg aggtgttcct atttgagtcg acaatttatg aatccagatg
     1021 aaccaaattt tatttggaaa aatcggaatg cagtaattga tatgaattgc atcattgagg
     1081 cagagcttcc agatattatg ccgaacaagc gatacgaaac ttacacgatt cagggccgaa
     1141 gcggagaatt gacggagacg tttgatgact atgaaccttt tgatttggaa atagaaggga
     1201 tcactattcc acattcgaaa ctgagagaag tcaaacgatg gctcactggt aaaagccaat
     1261 taatcacaca taatgatcca gacaagtatc tagaggctat ctgcagcatg gataaagaag
     1321 tccctttcga aaacgagtgg ggcttctttt atacattcga tgttactttt cgttgtcagc
     1381 cactcaaaag aaaaatcggt gaagcaccta aaatttttca ttctagtgtt atggatattt
     1441 ttgatcatgg tgacgaaatt gcacatccct atctggaaat agaatcgaac ggaggagata
     1501 taacgatcgc tgtcggtgat aaaagcctaa caatacttaa tacgttagct gcaacaatcg
     1561 tggttgatac tcagcttgga aaagcaatac aagaaggttt gaatttattt acgcgtggtg
     1621 attggccagt attgcagcct gaatggaatc aggtaaaaat atccggaaaa tttaaagaag
     1681 tgcggttttg gaataggagt gtgtatctgt gacgcaagaa tttatttatg cttataaaaa
     1741 aatgccagat gatttgagcg tgaacggagc gtcgttagtt gattgggaag atttgccaga
     1801 aatcaatcgt gtgctgaatg gccaatatcg tttttatggt aactattcaa gaagcggaca
     1861 gtatcgctct tatttaaaaa aaggaaattt tataaaagcg aaggtaccag atggatcatg
     1921 gcaatatttt gaaatttata atgtcaaaaa gaatttgacc tccgtttctg taacagctcg
     1981 gcatattggt tttatggcca ataagaactt tattgtcaag tcgtttacgg ataacggcaa
     2041 tgggtctcag atcatgacca atcttaaaaa cagtttggca tttgatcaaa aatttaacta
     2101 tctttcgaat gtagggacaa cgcaccaatt tacagcaaga caagtcgcgc cagttgaagc
     2161 aatcattggt tcaaataacg gtaatcaaaa tttgactggc gtgactagcg cggaacttga
     2221 tatggataac tatgatttga aactggtaaa acagattgga tcagataatg gttttcggat
     2281 tgattttggc atcaatttag aagcaatcga agaagaaatt gatgaagaat caattgtcaa
     2341 tagtttgtat ttggttggcg gtgttcctga taacgattat gatgaagaca aagaacctat
     2401 cgaatatggt tatttagaaa ttgatggagt aaccaacgag aatcgacgta ttgccaaacg
     2461 tgaaaacgga gattgtaaaa cagttgatga attgatcaaa tggggcaaaa ccttatttga
     2521 caatgatcgt atccatgagc caaaagctac tcatacagtc agtatggtgg cgttggaaca
     2581 cacactcgaa tacggtgaca tgtatcaaga gctagcgtct ttacactttg gtgatgtcgc
     2641 tcatgttagg gcgaaagagc tagatatcga gataaaagag cgtatggtcg aatacactta
     2701 tttcccaacg cttgggaaat ataaggatat tgttttaggt aatgatttat cactgtacac
     2761 ttcggcagta aatactcaag cacaagaatt gaaaaagaaa atcgacaatc gaacagaaac
     2821 gttagttcaa aatgtgttga atgcgacggc atggatcact ggtaattctg gtgggcatgt
     2881 cgtttttcgt ccagaaaaag ctccatcaga gattcttatc atggatactc caaatgtggc
     2941 gaatgcaaag cgagtgtggc gttggaattt aaatggctta ggttattcag acaacggagt
     3001 aaatggcccg ttcggtattg ctatcacatc aaaaggagaa attgttgctg acttcatcaa
     3061 agtaggaaca ataaatgcag aagtattcga aacttccttt aatgcttatg gtgatgtgtt
     3121 aaaacttgta aaaggcacgc ttcaaatttg gaatgaaaac aaaaaaatca tggagctaac
     3181 caaaaaaggt atggaattct ggaatagaaa agaatccatt ggaacgatcg gaacaacaga
     3241 ttcagcagga aatccatttc caaatgctgt aacacctaca cctcttgagg aaaattcttt
     3301 ggtaatacgt acaaatggcg atggaaaata cattttaatt tctccaactg cagaaaaagg
     3361 atttgtgtta ttaggaaacg gaaaagcata ttatttcggt gatttagata ttcagggaaa
     3421 acttactgtt agaggtaaag aagtgatacc aggacaaaat ggcggtccat ctggcggagg
     3481 agaaacacct ggtgggtatc ctgatgaatt aaaaacagat gcagaaaaga gagcttggag
     3541 aatatacgat attttgtgta ataacggatt tacgaaacaa tctgcatgtg gaatattggg
     3601 gaacattcaa caagaaacag gaggaacttt tgatcctgat actgttcaaa taggtggacc
     3661 agcatacgga ttagttcagt gggatggttc ttcatatcct cttgttggtc cagcaacatg
     3721 ggatggaaaa gtttatgttc aaaacttatt taatgctgca ggtattaaag aaccaataac
     3781 gagcttagat gcacaagttc gattgcttat ttggacattt acaaatggac aatggatgg
//
LOCUS       DACDHL010000162         3839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDHL010000162 DACDHL010000000
VERSION     DACDHL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.166"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..996
                     /locus_tag="IV228_002787"
     CDS             <1..996
                     /locus_tag="IV228_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8718625.1"
                     /translation="ESVQVSLLPFFEKATSLLQEFSKWWGSLDQDTKNWIITIAGIAA
                     AVGPVLIVLGSLMGSVTKIVGGVKSFIGVWQGLAGLFGISGGWFALAVIAIGALVTGL
                     IWAYNNVKWFHDGMNSFFKGVSDVAVEIFNFLGGYFGGFFDGVIANFNNFYNAGKRIF
                     SGFLDFITGVFTGNWSKAWQGLVDIFGGIFDGIVAVGKAPINAMIGLINGFIGGLNNI
                     KIPKWVPGVGGKSFSISKLPYLAQGGHLINGQAIVGEAGPELLTAKNGKTTVTPLSDE
                     EKRRGIGGKVSGGSIEQHIHIGKVDANNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            1008..1712
                     /locus_tag="IV228_002788"
     CDS             1008..1712
                     /locus_tag="IV228_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8718626.1"
                     /translation="MKPDEPNFIWKNRNAVIDMDCIIEGELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            1709..>3839
                     /locus_tag="IV228_002789"
     CDS             1709..>3839
                     /locus_tag="IV228_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718627.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAMTSKGEIVADFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNDNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGDL
                     DVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT         1240 a          567 c          883 g         1149 t
ORIGIN      
        1 gaatcagtcc aagtaagcct tttgcctttt tttgaaaaag caacttcctt attacaggaa
       61 ttctctaaat ggtgggggtc tctagatcaa gatacaaaga attggatcat aacaattgct
      121 ggtatagcgg cagcagttgg accagtacta atcgttcttg gttcacttat gggatctgtt
      181 acaaaaatcg ttgggggggt taaaagcttt ataggagttt ggcaaggact tgctggttta
      241 tttggaatat ctggaggctg gtttgcatta gcagttatag ctattggcgc attagttact
      301 ggactaattt gggcatataa taacgttaaa tggtttcatg atggaatgaa ttctttcttc
      361 aaaggtgttt cagatgtagc tgttgaaata ttcaattttt taggtggtta ttttggtgga
      421 tttttcgatg gtgttatagc aaatttcaat aatttttata atgctggaaa acgaattttc
      481 agtggttttc tagattttat tactggagta tttacaggaa attggtcaaa agcatggcaa
      541 ggtctagttg atatttttgg aggtattttc gatggtattg ttgctgtagg gaaagctcct
      601 attaatgcca tgattggatt gatcaatgga tttattggtg gattgaataa tataaagata
      661 ccaaaatggg ttcctggtgt aggtggaaaa tcattttcta tttctaaact gccttattta
      721 gcgcaagggg gtcatctgat taatggacaa gcgattgttg gtgaagctgg tccagagtta
      781 ctgactgcaa aaaatggtaa aactacagtt actccgttat ctgacgaaga aaaacgcaga
      841 ggtattggcg gaaaagtgtc tggtggtagt attgagcagc atatacacat tggcaaagtc
      901 gatgcaaaca atccatctga gttagatcgt atgaatcgta aatttgctaa agcaaaccaa
      961 caggctattt atgatttggg gggtgttcct atttgattcg acaatttatg aaaccagatg
     1021 aacccaattt tatttggaaa aatcggaatg cagtaattga tatggattgc attattgagg
     1081 gagagcttcc agatattatg ccgaacaagc gatacgaaac ttacacgatt cagggccgaa
     1141 gcggagaatt gacggagacg tttgatgatt atgaaccttt tgatttggaa atagaaggga
     1201 tcactatccc acattcgaaa ctgagggaag tcaaacgatg gcttactggt aaaagccaat
     1261 taatcactca taatgatcca gacaagtatc tagatgctat ctgcagcatg gataaagaag
     1321 tcccattcga aaacgagtgg ggcttctttt atacattcga tgttactttt cgttgtcagc
     1381 cactcaaaag aaaaatcggt gaagcaccta aaatttttca ttctagtgtt atggatattt
     1441 ttgatcatgg tgacgaaatt gcacatcctt atctggaaat agaatcgaac ggaggagata
     1501 ttacgatcgc tgtcggtgac aaaagcctaa caatacttaa tactttagct gcaacaatcg
     1561 tggttgatac tcagcttggg aaagcaatac aagaaggttt gaatttattt acgcgtggtg
     1621 attggccagt attgcagcct gaatggaatc aggtaaaaat atccggaaaa tttaaagaag
     1681 tgcggttttg gaataggagt gtatatctgt gacgcaagaa tttatttatg cttacaaaaa
     1741 aatgccagat gatttgagtg ttaacggagc gtcgttagtt gattgggaag atttgccaga
     1801 aatcaatcgt gtgctgaatg gccaatatcg tttttatggt aactattcaa gaagcggaca
     1861 gtatcgctct tatttaaaaa aaggaaattt tataaaagcg aaggtgccgg atggttcgtg
     1921 gcaatatttt gaaatttata atgtcaaaaa gaatttgacc tccgtttctg taacagctcg
     1981 gcatattggt tttatggcca ataagaactt tattgtcaag tcgtttacgg ataacggcaa
     2041 tgggtctcag atcatgacca atcttaaaaa cagtttggca tttgatcaaa aatttaacta
     2101 tctttcgaat gtagggacaa cgcaccaatt tacagcaaga caagtcgcac cagttgaagc
     2161 aatcattggt tcaaacaacg gtaatcaaaa tttgactggc gtgactagcg cggaacttga
     2221 tatggataac tatgatttga aactggtaaa acagattgga tcagataatg gttttcggat
     2281 tgattttggc atcaatttag aagcaatcga agaagaaatt gatgaagaat caattgtcaa
     2341 tagtttgtat ttggttggcg gtgttcctga taacgattat gatgaagaca aagaacctat
     2401 cgaatatggt tatttagaaa ttgatggagt aaccaacgag aatcggcgta ttgccaaacg
     2461 tgaaaacgga gattgtaaaa cagttgatga attgatcaaa tggggcaaaa ccttatttga
     2521 caatgatcgt atccatgagc caaaagctac acacacagtc agtatggtgg cgttggaaca
     2581 cacactcgaa tacggtgaga tgtatcaaga gctagcgtct ttacactttg gtgatgtcgc
     2641 tcatgttagg gcgaaagagc tagatatcga gataaaagag cgcatggtcg aatacactta
     2701 tttcccaacg cttgggaaat ataaggatat tgttttaggt aatgatttat cactgtacac
     2761 ttcggcagta aatactcaag cacaagaatt gaaaaagaaa atcgacaatc gaacagaaac
     2821 gttagttcaa aatgtgttga atgcgacggc atggatcact ggtaattctg gtgggcatgt
     2881 cgtttttcgt ccagaaaaag ctccatcaga gattcttatc atggatactc caaatgtagc
     2941 gaatgcgaaa cgtgtgtggc gttggaattt aaatggctta ggttattcag acaacggagt
     3001 aaatggcccg tttggtatcg caatgacgtc gaaaggtgaa atcgtagctg atttcattaa
     3061 agttgggatt attaatgcag aagtccttga aagctcaatt aatgatctgg gcgatgtgct
     3121 aaaaattgtc aaaggtacat tacaaatttg gaatgataat aaaaaaatca tggaattaac
     3181 caaaaaaggg atggagttct ggaataccaa agaatccatt ggcacaattg gtactactga
     3241 ttctgcaggc aatccttttc ctggggcttc tactccaacc cctattgaag ataattcttt
     3301 agttattcgt acaaatggag acggcaaata tattttgatt tctcctaaag taggaaaagg
     3361 attagtttta ttaggaaatg gtaaagcaat ttattttgga gacttagatg tacaaggaaa
     3421 acttacagtt aaaggaaaag aaataattcc tggacaaggt ggtggtccat cgggaggtgg
     3481 agaatctact ggtgggtatc ctagtgaatt aaaaacagat gcagaaaaga gagcttggag
     3541 aatttacgat attttgtgta ataacggatt tacgaaacaa tctgcatgtg gaatattggg
     3601 gaacattcaa caagaaacag gaggaacttt tgatcctgat actgttcaaa taggtggacc
     3661 agcatacgga ttagttcagt gggatggttc ttcatatcct cttgttggtc cagcaacatg
     3721 ggatggaaaa gtttatgttc aaaacttatt taatgctgca ggtattaaag aaccaataac
     3781 gagcttagat gcacaagttc gattgcttat ttggacattt acaaatggac aatggatgg
//
LOCUS       DACDHL010000163         3678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDHL010000163 DACDHL010000000
VERSION     DACDHL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.167"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IV228_002790"
     CDS             complement(150..1337)
                     /locus_tag="IV228_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8718628.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="IV228_002791"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="IV228_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="IV228_002792"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="IV228_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8718629.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..3472
                     /locus_tag="IV228_002793"
     CDS             3305..3472
                     /locus_tag="IV228_002793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718630.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            3526..>3678
                     /locus_tag="IV228_002794"
     CDS             3526..>3678
                     /locus_tag="IV228_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8718631.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT         1051 a          684 c          735 g         1208 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgcttttac tggttctgtt gcaaagtttt aaatctacta
     3481 tcaaataagg tagaataata gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa
     3541 ggaaagcaat ttcagcagga tgtgattatt gtagccgtgg gctactatct tcgttataac
     3601 cttagctatc gtgaagttcg agaaatctta tatgatcgtg gcattaacgt ttctcatacg
     3661 acgatttatc gttgggtg
//
LOCUS       DACDHL010000164         3638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDHL010000164 DACDHL010000000
VERSION     DACDHL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.168"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            202..1151
                     /locus_tag="IV228_002795"
                     /pseudo
     CDS             202..1151
                     /locus_tag="IV228_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1196..1829)
                     /locus_tag="IV228_002796"
                     /pseudo
     CDS             complement(1196..1829)
                     /locus_tag="IV228_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(2089..2637)
                     /locus_tag="IV228_002797"
     CDS             complement(2089..2637)
                     /locus_tag="IV228_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718632.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2638..3495)
                     /locus_tag="IV228_002798"
     CDS             complement(2638..3495)
                     /locus_tag="IV228_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8718633.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
BASE COUNT         1099 a          694 c          549 g         1296 t
ORIGIN      
        1 ttcttgcagg atgaggtttt ttatataaac tttatttgat ataaagaaaa ggcattcata
       61 attgtgctat actactaaaa aatgataata cagtgaatta gattagcgct ttattaaatt
      121 aagaggacag catatcgata ttgcttcctt ttttttgcta tttatttaag ggatagaagt
      181 ttagaaagga ctgtttataa gatgaaaaat gatatagtga cttatgcaaa cacaaaatgt
      241 gaagaaattg gcatcagtaa tttagaagag tgcttttttg acacaaatga cctgtcgcaa
      301 tttttaagaa cgggaagaac aaatgccagt aaacttgcca atgacttagt tgcaaagaac
      361 gttctattta aaatcaatac tcgtccagtg ttatttgcca tcaaagagcg ctttgaagag
      421 cactttttga ttccggtaaa agatgtatac gaaagtatat ctgaattaaa aacagacttg
      481 gagcccaatt ctacagattc cattctacaa aagatgattg ggtatgatat gagcttatcc
      541 gatgcggtgg aacaaatgaa aactgctgtc ttctatcctg gaagagggct tcctatgatg
      601 atcactggag atactggagt tggaaaaagt ttttttgttc atactgctta tcagtttatg
      661 aaaaaagtga acgttattca tgagaatgct ccttttaaag tacttaattg tgcgcaatac
      721 cataataatc ctgaattatt gtcggggata ctatttggct atgttaaagg tgcttttacc
      781 ggcgcagatg aagatcgaaa tggattgttg caagatgcag acggtggagt actgttttta
      841 gacgaagtac acagattgcc tcctgaaggg caagagaaac tatttgttta tatggatggc
      901 ggaacttatt ctagagtcgg tgaaactatc catcacgcct ccaatgtacg tttagttttt
      961 gcgactacag aaggcaaaga ggattttctg gaaaccttta tgcgaagaat ccctatacat
     1021 atccatatcc caaatattga ggaacgaggt attttagaaa aaaacaaatt atcgaaaata
     1081 ctctctttag cgaaagcaaa aactttagca aggctattga tgtttctcct aaagtcacta
     1141 gtgttgcata aatatagtca gaatattctg aattattatt ttcttgaaaa aagtaaaaaa
     1201 aatcgtatcc caaactttca tctacatttc cagaaagatc ttcatgttga taatcaattc
     1261 ctgaatcaat gaccgctacc ttcacatcct tagaaccctt ggtttcttcc caagcggatg
     1321 gcatgctaat ctctcttaaa ttagcttgtt tactccaatt aggatcgtta tcgatttcct
     1381 aaagaatcag ttgtatttgg ctcctcaaca tcattcaacg gctctagttc aaccacatag
     1441 tttggctctg cataattgac catatcagaa gcattaaatg ttcttactgc ctcttccaca
     1501 ctaatttctt cgttcaattt cactaataat acttgatctc gcttattata atcgctttca
     1561 tttgtcctaa gagtcaaatc tttttccacc ataactttta cacccaataa attttgcggt
     1621 ttatttttct ctgttgaaat tctttgtgta gcctttaaac tgacaattag ctcattgggt
     1681 acatactcta tttgagagtc atctaattgg ctcataatcc tttctttttg gttagtatct
     1741 gcagttactt gtttagtatg agccatgaac ccaaacatac tcaccccaac aaccaatcct
     1801 aaatatattt tctttttaaa ctttttcatc tacatctcct cctaatagtt ttattttaga
     1861 gttagctcta ttacttattt actaactcaa caacagatta tcatacactt attaatgaaa
     1921 tcacaatatc tcattaaaac acaccctaaa atcataaccg ttcctatgct cattttttgg
     1981 attttttaat cacgtaacca gaaaaataaa tcccctctat ttcacgcaaa tcaaagaaga
     2041 actaagtaac taccgtttaa aggtagttac ttagttcttc tttgattatt atctaacttt
     2101 attattcatt ccccaccctt tgcgcagtga gtcttcggta tcttgtttta tctgactggt
     2161 agttcttatt atttctccac gatcatctaa ttctggaaca attggatatt tttctctgaa
     2221 caagtctagt tctttcacca cgtcattaaa caaatcttgt ttatcttctt gcgtaagatt
     2281 ctgtttaata tactcatcta aagcttttct aagcaattca tctcttgtca tattagtctc
     2341 atacttttct actaaaactc tggaagtcgc atttaacaaa gtcgacagtt ttaaaggatg
     2401 cttggtggaa taaaccttat caaactttct gattggtgcg ccaacttttt ttgttcttcc
     2461 tctactctgt ctttcaacat tggaggaagt ttttatttct tgaacttggt gcacttctaa
     2521 ttgcttattt tcagatggtg taattgcttg ctgctttttc tttgcttctg cttctttttt
     2581 tattttggcg agtcctttgt tgttcccaaa aatatcaagt cctttgatgt cagccaatta
     2641 ctccacctcc acataccaat tctcgtgttc ttccaattct ttcaaaatac tgataaataa
     2701 ttcgtggctt ttgcgatccc attgttttaa gagacctttc tttgtcttct ccatcgtaat
     2761 acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt acaacatttt taatcaaatg
     2821 ttccccataa atctcttggg cttgtttata catttcctga tctactgcgt caccgctttc
     2881 caacatgaaa ggaataacac cagcaacata taaatttgta tcaaattctt tcacaaaaaa
     2941 gtccatatac tcaagatatt gcatcgcatt tctcatcgat tttacttggg tctgtaagac
     3001 gacgattgcc caatccgcaa tgtacatcgc ggatttacta tatacagaaa tagaaggtgg
     3061 gacatctaaa tagatgacgt caaactcatc aaataatggt tctagctttt ctttcatata
     3121 tttgaattgg gataatgaat cattatattg gtctttgatc caatcatcat acatttcaaa
     3181 ggacatgtct gctggaagta tatagaggtt attgattact ttatagacag cctctttagg
     3241 tggaagattg tttatcaaca cgtcagtcac agtattttcg taaacaatcg gttcaccttc
     3301 tccgcctcct tcttcgttag tgaagttcat atgcgttaag ttcatcacat cagaagtatt
     3361 tccttgcata tccaagtcaa ttatcaaggt tctttttcct tcattaactg ctgattcaaa
     3421 ccctaacatt tgcgcgcacg tacttttacc tacgccacct ttaaaattac cgattacata
     3481 tttcttcgct accacgttcc tacctctttt cttttagcaa tattcatttg atactattat
     3541 aacatattta tttttagtta ctaccaaaaa aaagatatat atgtactacc ttataattaa
     3601 taagtaaaag gtagtacata aataaggaaa agctgaaa
//
LOCUS       DACDHL010000165         3624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDHL010000165 DACDHL010000000
VERSION     DACDHL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.169"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..1078
                     /locus_tag="IV228_002799"
     CDS             134..1078
                     /locus_tag="IV228_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718634.1"
                     /translation="MFKKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIFINHYSLEKE
                     LTKEVQDEYISARLPDKAREIKDFISYSIGCIFGRYSLDEDGLIFAGGEWDPSRYQKF
                     KPDTDNVLLITDDEYFKDSQIDIVNRFIDFVEITFGKETLEENLQFIADTLGGKGTSR
                     EIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQLGKVRTEYL
                     HELQKAYDNRIELRQQQISTSDNPKERNAMTKEIVKIQKQLKEVREYDEKLGHLALKR
                     IELDLDDGVIVNYDKLQRDPKTGEKFQIFAKGVKEPKK"
     gene            1194..>3624
                     /gene="pglZ"
                     /locus_tag="IV228_002800"
     CDS             1194..>3624
                     /gene="pglZ"
                     /locus_tag="IV228_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HAP8718635.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYY"
BASE COUNT         1314 a          506 c          693 g         1111 t
ORIGIN      
        1 tcttaaagtt acatctgaga gaaaattaaa aaaaataaga gaaaaagtgg atgaaaatat
       61 cgatatagct agaaaagact ataatgctct tgaaacgtca tacgagttta gaagaaacat
      121 tttgttaagt tatatgttta aaaaagatct ctcagaggca tttaactgtc taagtaggca
      181 gttggttaat caatttaata ctctaaaatc gaatgaagag gaactgaacg acatattcat
      241 taatcattat tctcttgaaa aagaattaac aaaagaggtt caagatgaat atatctctgc
      301 aaggttacct gataaggcga gggaaattaa ggattttatt tcttattcaa taggttgtat
      361 cttcggtcgt tactccctag acgaagatgg tctcatcttc gctggcggcg aatgggatcc
      421 ttcccgctat caaaaattta aacctgatac tgataatgtt ttactcatca ccgatgatga
      481 gtatttcaag gatagccaaa ttgacattgt caatcgtttc attgatttcg ttgagattac
      541 ttttggtaaa gaaacccttg aagaaaatct ccagtttatc gctgacactt taggtggcaa
      601 agggacaagt cgagaaatta ttcgtcgtta cttcgtacaa gatttttaca aagaccattt
      661 aaaaacatac tccaatcgac caatttattg gcaattggac tcaggaaaac aaaatggctt
      721 taaagcgtta atgtacttgc atcgctatac accagatcaa ttaggaaaag tacgtactga
      781 atacctacac gaactacaaa aagcgtatga caatcgaatc gaattgcgtc agcaacaaat
      841 ttcaacaagt gataacccaa aagaaagaaa tgcaatgaca aaagaaattg taaagattca
      901 gaagcaatta aaagaagttc gtgagtatga cgagaagttg ggtcacttag cattaaaacg
      961 aattgaatta gacttagatg atggtgtaat tgtcaattat gataagttgc agcgtgatcc
     1021 taaaaccggc gagaaattcc aaatttttgc aaaaggggtc aaagaaccaa aaaaatagtt
     1081 atctaagaaa gttgttttct aacaactttc ttttttattc ttaaattgcg tgcgaaatta
     1141 tccaaattat gtcaatataa ggggtataga aagagaacga ttaggtgaga ttgatgacag
     1201 atataaatac gcaacaaatt gaagaaaaat taaatcaacg ttttcctaat tatggatgta
     1261 ggaaaatcat tttttggttt gattcaaatc aggattttct tgaagaaatt gactcattgg
     1321 tcattcaaaa tgcaaaaatc tatcgattag aaaaggacgc acaatttaaa actaagcgtt
     1381 tattggaata tgaagatccc gaaactaact atttaatcta tgcgccattt cctcgttggg
     1441 atgataaaga tgaagataat catttgttga gtgtattgaa ttattcagag gaatttagtg
     1501 cagaccgctt agcgattatc atgaatgaat tggctattcc acttagattt catcagacag
     1561 ttaaacagta tcgtaatttc tttaatgcaa aggatcgcat gagaagtttt ggtgaattta
     1621 gtacgcatat gacaattaat aaatcagaaa cgattgaatt aatgataatg gctgttttgt
     1681 tgaaatcgaa aagtattcga ttaaatgatt tactgcgtgt agttctaaaa agctttacag
     1741 aaagtaggga gaatccttta agggaacttg ataagtatca gttaacagct aaattttggg
     1801 aatttattca aatgaaattt ggctttacgg atagccagcc aacattggaa aagctgacga
     1861 tgtgtcttta tttgaactat ttttattatc aaattgatca agagatgccg aagaagttca
     1921 aaggatttga aatactacat ttgaaagcaa acattattac tttcatggat cagtttatga
     1981 atgatacacg ttacatggaa agctttgaac agctatctaa aaaagtatat caggctattg
     2041 atggtgatgg attattagac agtatagaaa ttgatgatct aattgaagca gacgtatttt
     2101 ctgagattca tgcaaaaata ttaatgtttt atgcagaacg attaaaggct tctgacacta
     2161 caacgaaaat tgcaggacaa acaatttttg atagtttaaa gcagcgcgaa aaaatgcatt
     2221 tcgcatcgga ttatatttat gagtatcaat tattgaaaca tgcttattat cttgtggata
     2281 aaaacagatt gtgcttgccg atgaacatgg ataatttgct aactcaatat gagaaggaaa
     2341 tgtattgggt tgatacgcat tatcggaaat ttatttggaa tagtgatcat atagaagaaa
     2401 agcaaaaata tggccaattg tatcgattag ttgaagcaaa ttattcgaag tttcttgatc
     2461 aatcaggaca aatttggaat caaacttttg aatttaagaa tcgtccatcc attcgaaaat
     2521 tttataatca agctgtaatg aaacgctctg ttaaaactgt agttattatt tcagatgctt
     2581 ttcgatatga gttagggatg aatttagaac gtaagttagt tagagaaaag aagtattcaa
     2641 caaacatggc tagctttctt tctgtattac cttcagttac tgaattcggt aaagccgcct
     2701 tattaccaaa caatcaacta acttacaatg aaggaacaga agtactgatt gatgggaaaa
     2761 aaacgaatgg tttatccaat cgaaatgcga ttctccagca acgagatgca aatgctgtag
     2821 ctttaaatta tgatgatgtg gttaagatga ataaggctga ggtacgagaa gtatttaatg
     2881 gcaaatcagt catttatatt tatcacgatc aagtggatag aacaggtgat cactcgaatg
     2941 aaaatcgagt ttttgaggcc tgtgatttag cgataagcga aatttcccga cttattaatc
     3001 gcttgcataa tgatggcagt atctatcgtt ttatagttac ggctgatcat ggatttattt
     3061 ataaaagaag ccacgttgaa gaagtagata agattgaaaa tccatctaga aatgaaaaag
     3121 atcgtgtaga acgtcgtttt attcttagtg aagaagaata cgaaataatt ggtacgaaaa
     3181 attactcttt gggagattct ttaggaaatg atgacactag aaacatttgt gtcccgatta
     3241 cttcaagtat ttttaagaaa gctggcgggg gacaaagcta cgttcatgga gggtcatcta
     3301 ttcaagaaat gctagtccct gttctagaaa ttacggtagc caaaggtagc aatgcaaagg
     3361 aaccagtagc aattgaatta atgactagca accgcagaat tacaggatta tcaaccacgt
     3421 tagaattcta tcaaaaaaat gcaattagtg atgtttatga ccccgcatct ttctggcttt
     3481 attttgaaga tgaatatggt gaacgtatct caaatgaaga aatctatgtt gcggattcaa
     3541 aaacagaaga tgcaaatcag cggtttacta aatttgtttt tgaatttgta aatcgtcgat
     3601 atacagttga agatacttat tatt
//
LOCUS       DACDHL010000166         3585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDHL010000166 DACDHL010000000
VERSION     DACDHL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.170"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            30..1073
                     /gene="pheS"
                     /locus_tag="IV228_002801"
     CDS             30..1073
                     /gene="pheS"
                     /locus_tag="IV228_002801"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8718636.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            1078..3498
                     /locus_tag="IV228_002802"
     CDS             1078..3498
                     /locus_tag="IV228_002802"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8718637.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
BASE COUNT         1238 a          614 c          798 g          935 t
ORIGIN      
        1 tatttggaat ttaaaggagg aacaagcaca tgtctctaca agcacaatta gagagcttga
       61 aagaagcaac gctcgtcaaa atcacagcgg cacaagattt aaatgaatta aatcaaatac
      121 gtgttgaaac gttaggaaaa aaaggaccga tcacagaagt attacgtggg atgaaagatc
      181 taagtccaga agagcgacca aaagtcggaa gtttcgcaaa tgagatccga gatcttttga
      241 cagaagagat cgtgcgaaaa aaacaagtat tagaagaaga agcgttaaat gcagctttgg
      301 caaaagaaac aatagacgtg acactacctg gtagaaaagt aaaacatggt acacgtcata
      361 tcgtgacaca aatcatggaa gaaattgaag atatttttgt gggtatgggc tatcagatcg
      421 tagaaggata tgaagtagaa tctgatcatt acaatttcga acggatgaat ctgccaaaag
      481 atcatccagc aagggacatg caagatactt tctatatttc agacgagata ttgattcgga
      541 cacatacttc accagtccaa gcacggacaa tggaaaaaca tgatttctct aagggtgctc
      601 tacgaatgat ctcgccaggg aaagtcttcc gcagagatac agatgatgcg acccacagtc
      661 atcagttcca tcaaattgaa gggctagttg ttgataaaaa catcacaatg ggcgacctta
      721 aaggaacatt agaagtcgta atgaaaaaaa tgtttgggga agatcgtgaa attcgtttgc
      781 gtccaagtta tttcccattt acggaaccat cagtagaagt agatgtcagc tgtttcaaat
      841 gtggtggtgc cggttgtaac gtatgtaaat acactggatg gatcgaaatt ttaggagctg
      901 gcatggtaca cccgaatgtg ttgaagatgt caggaatcga tccagaagaa tattcaggct
      961 ttgctttcgg ccttggacca gaccgcgtag ctatgttgcg gtatggagtg aatgatatcc
     1021 gtaatttcta tcaaaatgac ttacgtttct taagtcagtt caagggggag taagaaaatg
     1081 ttagtttcat ataaatggct caatcgttat gtagatttat cgaatgtaac accaaaagaa
     1141 ttagcggata agatgtctgt gacaggaatc gaagtagagg gagtaacggt tccagaagaa
     1201 ggcttgaaaa aaatcgttgt cggtgaagtc aaggaatgcg taccacatcc agattctgat
     1261 cacctttcta tttgtcaagt cgatattggc gaagacgaac tgtctcaaat cgtttgtgga
     1321 gcgcctaatg tgaaggttgg aatcaaagtg attgttgcac ttcctaactc acgtatcgct
     1381 ggtaatgtaa aaatcaaaaa aggaaaaatg cgtggccaag tatcaaatgg catgatttgc
     1441 tcattacaag aaattggcta ttcagatagt gttataccga aagaatacgc tgaaggtatc
     1501 tattatatgc cacaagaagc tgtaaatggc gatccagttt ttccttattt ggatatggat
     1561 gattcgatta ttgaattatc tatcacaccg aatcgtgcgg atgcattgtc aatgcgtggc
     1621 gtagcttatg aagttggggc aatttatcgt cagactccta cattcgatga cgaaaagctg
     1681 gtagaatcct cacgtcctgc tagcgataaa attcaagtgg aagttgaaga taaagaagca
     1741 gttcctgctt atcaaatcag aatcattgaa ggagtaagaa tccagccaag tcctcaatgg
     1801 ctgcaaaatc ttttgatgaa cgaaggcatt cgtccaatca acaatgtagt agatgtgacg
     1861 aactatattt tgcttttatt cggacaacca ctacatgcat ttgactacga caaattgaaa
     1921 tcaaaagaga tctttgtccg ccgtgcaaaa gaaaatgaaa cattagtaac actagatgga
     1981 gaatctcggg aattgacaac tgacaatctc gtcattacta acggcaaaga accagttgct
     2041 ttagctggtg tcatgggagg gttagactca gagatcacga atgaaacaac aacagttgca
     2101 ctagaatctg ctttatttga tccaatttcg attcgtcgta cagcaaaaca atttaatttg
     2161 cgtagtgaat cctctgcacg ttttgaaaaa ggaatcaacc atgcgactgt tggagaagcc
     2221 agtgaggttg ctgcagcaat gatcgtttct cttgcgggtg gggaagttct acaaggcgcg
     2281 gtcaaaggct cagagatcaa agcagaagac gtatcagtga agatttcact tgaacgcatc
     2341 aatcaatatt taggaacatc tcttgtcgtt aaagaagtaa atgagatttt tgaagcactt
     2401 ggatttagct atgaagaaaa ggagtatgtt tattccgtag tagtaccacc aagacgctgg
     2461 gatatccaaa tcgaagcaga tattatcgaa gaagttgctc gtatttatgg atacgaccag
     2521 ttgccatcta cacttccaag tggagaaacg gttgctggaa gcttgtcaaa agaacaagaa
     2581 actactcgta aaattcgtac aatctttgaa ggaagcggat tgagcgaagc aatcagttat
     2641 gcattgacaa cagaagaaaa atcaagacag tttgttataa atgaaagtaa tattacacgt
     2701 ttggactggc caatgagcga agaacgttct gtattacgaa tgaatttgat cagcggacta
     2761 ttagatgata tcagttataa cactgcccga aaaaatacag agattgcttt atatgaaatt
     2821 ggtcgggtgt tcttccaaga aaatgatcca cgtacacatc tgccgcaaga agtaaaacat
     2881 gcagcaatcg ctatttctgg tatttgggaa gaaaaagatt ggcagacgaa agctcaaaat
     2941 gtagactttt tcaccatcaa gggtctttta gaaaacttat ttgaacaact aggcatcact
     3001 gaagatgtcc attatcaaac agtgacagaa atgaaggaaa tgcatcctgg aagaactgct
     3061 gcgatttacc ttggcgaaaa atttattggt tttgtcggtc aagtgcatcc aacgattaca
     3121 aaaagttatg atattccaga aacatatgtt gctgagtttg atttatctgc tatagtggaa
     3181 gctgctcaaa aagggatcgt tttcgaagca gtgacaaaat tcccagcagt cagccgggac
     3241 atcgctttgc ttgtaaaaga aactgtgaca aatcaggaat taacagaagt tatccgttct
     3301 gctgccggaa gatttttaac agatatccaa ttatttgatg tctatcaagg agaaaacatt
     3361 gaaaaaggac ataaatccat ggcgtatagc ctgacattcg tcaacccaga agcaacattg
     3421 acagatgaag aaatcaataa agcaatggaa aaagtcgaaa aagcgttggt agaaaaccta
     3481 gaggcaagta ttcgctaaga aggaaaatat agtaagtaac gatcaagtag cttttgaaga
     3541 ccatacattt gaaaaaaaga tgtgacacaa cttcgtcaca tctct
//
LOCUS       DACDHL010000167         3551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDHL010000167 DACDHL010000000
VERSION     DACDHL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.171"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            390..722
                     /locus_tag="IV228_002803"
     CDS             390..722
                     /locus_tag="IV228_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8718638.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKAVVQCKKYI
                     IQKDKVSEEMIYYTGKRKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1313..1642
                     /locus_tag="IV228_002804"
     CDS             1313..1642
                     /locus_tag="IV228_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718639.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            1629..1970
                     /locus_tag="IV228_002805"
     CDS             1629..1970
                     /locus_tag="IV228_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8718640.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2084..2323
                     /locus_tag="IV228_002806"
     CDS             2084..2323
                     /locus_tag="IV228_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718641.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2390..2629
                     /locus_tag="IV228_002807"
     CDS             2390..2629
                     /locus_tag="IV228_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718642.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            2715..3386
                     /locus_tag="IV228_002808"
     CDS             2715..3386
                     /locus_tag="IV228_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8718643.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
BASE COUNT         1266 a          476 c          784 g         1025 t
ORIGIN      
        1 aaaggcccct gtaaggctcg ggagactggt aatctccgtc agtgtcctac aggaaagcat
       61 taagatgacg ccttaatgcg tgcccttcag tgttacggta ttacccgcaa cggactcagt
      121 ataacagata ttcaaaagtg ataccacctt ttttggctct attaaggtta aaaatacgtg
      181 atattgtaat gaatctatgc ctaagcataa tgttataaaa atgtccgtgt ggctctttgc
      241 cgtatgacga atgctgaaat tagagggaac gcatgtttgg atgcgttccc tcttttttgt
      301 ttgtcatgaa cgaaaatcaa ttctctattt ttgaaaaata acaagaaaaa cttattaaaa
      361 atatttaaag aaagtgagag gattatttta tgtcatatac aggaattttg agtctcgaag
      421 atatttgtca ttacggtaaa cggtgtaccg ctacagagaa aatcacgaag aaactatcaa
      481 ctgggcaaag caaagctgtt gtgcagtgta agaaatatat aatccagaaa gacaaagttt
      541 cagaagaaat gatttattac actgggaaac gcaagcaaat aattttaaaa gatcctatcc
      601 ctttaaaaga gctatatcca acaatcaaac atgtttatga ccaaaacggt gtactgattg
      661 gtcgaagaaa aaatggagtc ttgcgttgta ccgcaaaagg gatggggcgt ttaatcagct
      721 gaattaatca ctctcataac ttgtatttta tcgtttttcg cttgcttgaa aatattgtcg
      781 agacttctaa aggaacttag aatcattaga aaaataagga gtagacttga tctatcaaca
      841 attcctttcg gggggtgacg gggtaggttt ttacgattgg taacgtaaaa aattggtcaa
      901 tcgagtaaat gcccttgttt ggatagtggt gaaatgaatt tgtgtaaata atatgcacag
      961 agtaaacaag agggaattta gaagttagac aagttagaat agcgcctaga ttaatcatga
     1021 gcagctacta agggatctag gctttgacgc aaactcatgg aataaggagt aggtcttctc
     1081 gtaatgggga ggacgcacaa gaaattgtgt cgtgtgctgc agtacatgta gatacttgcg
     1141 aagacgttga tctttggatc aatcgagcat ttaccgtttt ctactagggt ttctcgtttc
     1201 ggacgccata aacgagtcga tttattttat atatttttga gtgaacgaaa aacgataaag
     1261 atattcgtta tgaagtaaaa ctaatttagt agatagcaaa ggtggtagag gaatgattca
     1321 aaaatttgcg aaaatgagta aaactgcatg gattgaaaag cttgaaaatg acttgagtgt
     1381 catgcgatta gctccaaaga aattcaaacg agagattgca gaaaaagaag caattttgtc
     1441 ggtattgaat gcggtagacg ataaatgtcc agtaaaaatt agtttttcag atatagcaac
     1501 aattatcgaa ggtatttatt atggtgaagt tgatgaagag atatcttcta caaaagattt
     1561 attagatttt ttggataaaa acaaagatac ctttatagta ctaactgatt atgaggaggt
     1621 tacaacaaat gaaaaattat gatccgaata tacgattggg cacacatact attaaggttt
     1681 ctttccaacg ttgggattac aaaggatttg tgacgtttag aagaggcgga aattgtaaag
     1741 gattagatgt tttagcatta gacgaggatg atttatacga tcaaacattg actgataatc
     1801 caattggatt cggcttgcta cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc
     1861 ttatgaatga taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt
     1921 atattgtcag cgttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg
     1981 aataaatttt acagataaag gcttagaagg agtcacgacg attaataaaa aggtagtatt
     2041 accttaggca ttgagtaagg aatttattta ggaggaaaat tagatggcgc gaccagtttt
     2101 agaggcgatg gatatggata ctttgacaaa aggaaggtat gtacagctgt tagctcgtgg
     2161 ttattcacct aaagatgtat tcaaatccct gtctaaagga acagatatgg aaaaagaaag
     2221 attacgaaaa gaattcgatt attggagaac ccataatgga atcaaggatt tgaaaccagc
     2281 acggccagta attaaacgaa agaaaaagat aacccaagac tgaagtgtag agcgtatgtg
     2341 atcataggct tttttatttt tgcaaaaaac aaggaaggta ggaaaactca tgaattatga
     2401 agaaatcgaa aacagaaaaa aagtgtcgaa agaaatggaa gaaaagttgc taaaaacgat
     2461 gaaacaaaaa caccttaaaa gattatctgt catgcaatat atcaatgata tgcagataac
     2521 aggaaaagag aaagcttgtt tgttaggttc aatgaaaaac tttgaacagt tgagacgaac
     2581 ttatgtgaaa aaaagttcta attgtcaact attgttagaa gtctcatgac attcattaat
     2641 agaattcatt tttaaaactt tttctatgga aatgagaaaa agattttttg ttttgtctga
     2701 aagggggtag ctggatgaga aaatggttat ttggtttttt aggagtcgct ttaatcgtct
     2761 tttgcagtgt gtttggctat gttagctacc aaaaacatga aggagaggtg tttaagcaaa
     2821 atatagaaaa aaagatgccc gtagatcaaa tcaatgcaca cgcaaagagt tataaggagg
     2881 atgcaacgaa cgtcaataat gatatgtctt tggggcaaat gttgtccata caaaaagaag
     2941 caatcgaaat gggcgtcaat aaacaagtat ttgcacaaat tcagattcct gcacttggtt
     3001 tagcattgcc aatttttaaa ggggcaaatc aatacacttt gagtcttgga gcggctactt
     3061 atttttatga agatgctgaa atgggcaaag gaaactatgt cctagcaggt cataacatgg
     3121 aaatgccagg tgttttattt tctgatattc aaaagttgtc attaggggag gtgatggact
     3181 tagttagtaa cgatggcgtc tatcgttaca aggtaacgcg taaatttatc gtacctgggt
     3241 actttaaatt aatcgatggc gttcctgaag agaactcttt cttgtctttg ccaaagaaag
     3301 gagagaaacc cttattaacg ttgtttactt gtgtttatac gtcacaagga aaagaaagat
     3361 acgtggtaca gggagagcta caataacaag gttttaatca acgcgagtcg ttgaaatatg
     3421 aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg aatgcttgtt
     3481 ggaatgttag gaatcattgc aggctgcttt caggggtatc aagtacatgc agaagatgtc
     3541 acgcaaaaaa c
//
LOCUS       DACDHL010000168         3490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDHL010000168 DACDHL010000000
VERSION     DACDHL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.172"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            161..961
                     /locus_tag="IV228_002809"
     CDS             161..961
                     /locus_tag="IV228_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP8718644.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            977..1855
                     /locus_tag="IV228_002810"
     CDS             977..1855
                     /locus_tag="IV228_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8718645.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1868..2848
                     /locus_tag="IV228_002811"
     CDS             1868..2848
                     /locus_tag="IV228_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8718646.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
BASE COUNT         1201 a          566 c          720 g         1003 t
ORIGIN      
        1 ttttcacaaa cattcatatt ataatgtttc tttttaataa aaagattgtt tttaagctaa
       61 ttattatatg tgtgttattg aactaactct tgacagataa tcaaaaagaa tttatagttg
      121 ttaccgtagt agctactaaa aataaagtga ggattaatca atggaaacga ttattcaaat
      181 catgaaaaaa tatgattttt cagagatgga gacaagagtg tataccacac tattagaaaa
      241 aggtaatctt acaggatatg aagtaagtaa aatctcaggt gtgccacgct cgaaagtata
      301 taatattttg gaaaaactat taaaaaagaa tttgattgtc gttaataaat cagaacctaa
      361 actttatcat gccatctcgg caaatgagtt tttagagaaa ttagaaaaaa gtgttaaaaa
      421 tgatctttct tttcttacca agaatctagg gatgatcaaa gaaaaagatg aagaagaaat
      481 gctgtggaaa gttgatggta tcgagtatgt tctggataaa gtagagcatt tggtgaaaaa
      541 cgcaaaagaa agtttgctca ttcaagtttg gcatgagaat ttcacggatt cactactaaa
      601 ggcattgcag caagctgaaa aaagagttga taagtttgta ctgattttat tcagttctac
      661 ccatgaatat gatcttccct tagaaaagta ttatattcat ggttttgaaa cagacaaact
      721 tgctgacttt ggagcaagat ggatcaacat cgtcgccgat gagcaagaag tcgtgtttgg
      781 taccattaac gaagaactgc aatcaaccga tgtcacttgg acaaaaaacc acgccatggt
      841 caatctagca aaagaatatg tcaaacatga tgcatataca ctaaaagtga ttgcagaatc
      901 tcctgaagaa cttaaagcaa aatatggtga gggctttgag ggaatcagaa agatttacta
      961 gtaggaggaa ttttttatga ttcggaatat tttgctgaca ctaattgtcg caatcaatct
     1021 atattttatt atttactttc taagagacct tttcaaaaac aaacaaaatt tcaaagaaga
     1081 accaggggat atgcgtctgt tgcccttcac gtcatttatc atcttctttc tttcaacatt
     1141 tggcgtttcc gactttgcta tcggaaccgt cctttatcca aagttgaaat gggtcagcat
     1201 gaaaaaacta cccggaacgt tgaataccca atgtgtagta ccggtagccg tcatggcatt
     1261 gtcttacatc actgccatca acgtagggat caagacacta gctgtttgta ttatctgcca
     1321 aattatcggt gcatatttag gaccaagatt tgtcgtgaag ttaccagaga aaacgatcaa
     1381 actgtttgtc ggcataggat tggtcattgc tgcattgttg atttttgcag gacaaatgaa
     1441 ctggattcca tcaaacggaa cagccagtga attgtatggc ggaaaattaa tcttggcagg
     1501 atttttactc tttgtctatg gtgcattgaa caatatcggc attggttctt atgcgttaac
     1561 gatggtaact gtctacttgc taggcttgaa tccagttgca gcctttccta ttatgatggg
     1621 ggcttgtacc ttctcagtac cgatcggcag tgttcaattc attaaattta atgaatacag
     1681 cagaaaaatc acacttttta cttcaacctt cggtgtattg ggggtattgg tcgctgtatt
     1741 cctagtcaaa tctttagata cctatatcct gaaatggatc gtgattttgg tcttactata
     1801 cagtgcatat acgatgttgg gcagtcaatt aaaaaaagtt acagcagcta attaaggagc
     1861 gattcaaatg ttatttctta aaaaagaaga tattatcaaa agtttttcaa tgagagaagc
     1921 catcgatgcg gacaaaaaag cactgtcatt gtattcagca ggaaaggcaa gtgtgcctct
     1981 tcgcaccaat atcgatgtgc caaaaagcaa cggacaaagc ttatatatgc ctgcttatgt
     2041 agaaggtggc gaaggcgcgc taggtgtcaa aatcgtttcc gtgtatcctg aaaacatcaa
     2101 gaaaaactta ccaagtgtac cagcaacgat gatcgtattg gatccagaaa cgggtatggt
     2161 atccgcttgt ttggatggta cttatttgac acaattaaga acaggcgctg ttcaaggagc
     2221 agcaacagaa cttcttgcaa gagaagatgc taaaattggc gcgctgattg gaaccggcgg
     2281 acaagcacag tcacaattag aagcaatgtt gaccgttcgt aaattagaag aagttcggat
     2341 ttttgatatt gattttgagc gtgccagcca atttgctgaa aaaatgatgc aacaattttc
     2401 tgtaacgatg cgcccaacaa aaaccagtca agaatgcgtt gaaggagcag acatcattac
     2461 aagtgtgacc acttcaaaac gtgcgacatt ctctgctgag tgggtcaaaa aaggtgcgca
     2521 catcaatgga gtcggtgctt acacaccgga aatgtgtgaa attccacgag aaataatcaa
     2581 agccgcagat gttgttattt ttgataccat ggacggtgtt ctaaaagaag ctggggactt
     2641 catttcaccg ctgcaagatg ggtatattca aagagacagc tatcatgggg aactaggcca
     2701 actgatcaat gaagaattga tcggtcggac aagtggagaa caaatcacga tcttcaaaac
     2761 agttggttcg gctgtattag atgtggtagt tgcgacagaa atcgttaaga aagctaaaga
     2821 aaataatttg ggaaaattga ttttctaatt aaaaaggctg aaattaaaac ctttattatt
     2881 gcgtttaaaa aaagaaaaca aagaagtttg taatcaaatg gaaactgacc ccataattta
     2941 aataaaataa aaaacatctc ttgttgaatt ctagtaatga agcgacccta aaacttagac
     3001 caaaaaatct aacttttggg tggagttgtc aagaaaaacc gggcacttag ttaaaaaata
     3061 agttggacgg gttatactaa aattgacacc aaattaagag aaagcgaaga tctggattat
     3121 gccaagacgt tcatttgatg aaatatttaa aattaccgct gtaaaactgg taacggaaga
     3181 agggttttct gttaaagaag ttagtctata gctagaagct cgctctaata gcgtgtattg
     3241 atgagttcaa gaagttgaaa aatatggata acgtactttt ctcggtagag gaagtcatga
     3301 aatccattcg cagaaatgaa attacacgct tagaaaaaga gaataaacgg ttaaaagaat
     3361 aggtagaact cctaaaaatt accgaagtct tcctgaaaga aaaggctctt cgtcaaattg
     3421 tgttggcgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt ctgtgcgccg
     3481 catatttttt
//
LOCUS       DACDHL010000169         3480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDHL010000169 DACDHL010000000
VERSION     DACDHL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.173"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            39..521
                     /locus_tag="IV228_002812"
     CDS             39..521
                     /locus_tag="IV228_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8718647.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            558..1568
                     /locus_tag="IV228_002813"
     CDS             558..1568
                     /locus_tag="IV228_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8718648.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1683..2396)
                     /locus_tag="IV228_002814"
     CDS             complement(1683..2396)
                     /locus_tag="IV228_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718649.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            2546..3480
                     /locus_tag="IV228_002815"
                     /pseudo
     CDS             2546..3480
                     /locus_tag="IV228_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT         1231 a          573 c          694 g          982 t
ORIGIN      
        1 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
       61 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
      121 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
      181 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
      241 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
      301 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
      361 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
      421 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
      481 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
      541 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
      601 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
      661 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
      721 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
      781 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
      841 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
      901 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
      961 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     1021 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     1081 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     1141 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     1201 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     1261 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     1321 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     1381 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     1441 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     1501 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     1561 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     1621 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     1681 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     1741 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     1801 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     1861 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     1921 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     1981 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     2041 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     2101 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     2161 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     2221 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     2281 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     2341 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     2401 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     2461 accttgtcta tggataggct acaattaact agacagaaaa attaaggtgt gtagactaga
     2521 agaaaacata ccaggaggaa tttttatgtc taagagaaca cgaagaactt tttcacaaga
     2581 attcaagcaa caaatcgtca atctttactt agctggaaag ccacgtgtag aaatcattcg
     2641 agaatatgaa ctaacggctt cagcatttga caaatgggta aagcaatcta aaacgagtgg
     2701 ttcattcaaa gaaaaagata atcttacgcc tgaacaaaaa gaattgttag aactacgtaa
     2761 aagaaaccag caattagaaa tggaaaatga tattttaaag caagcagcgc tgatattcgg
     2821 acgaagagac aagtaatcga tgcgaataag catctttacc ctatatcagc gatgtgcaga
     2881 atattaggtc tatcacgtca gtcctattat tatcaatcaa aaccaaagaa agacgaatca
     2941 gaacttgaag aagtagtcgc tgaagaattt atccgcagcc gaaaggccta cggctcaaga
     3001 aaaataaaaa aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga
     3061 atcatgaaaa atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat
     3121 tctacttgca atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac
     3181 ccattagaag cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat
     3241 gtctgtttca ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat
     3301 aaagatgccg ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt
     3361 gagatttttc atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta
     3421 acaacttttg acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DACDHL010000170         3459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDHL010000170 DACDHL010000000
VERSION     DACDHL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.174"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            586..918
                     /locus_tag="IV228_002816"
     CDS             586..918
                     /locus_tag="IV228_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8718650.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1537..1878
                     /locus_tag="IV228_002817"
     CDS             1537..1878
                     /locus_tag="IV228_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8718651.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            1992..2231
                     /locus_tag="IV228_002818"
     CDS             1992..2231
                     /locus_tag="IV228_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718652.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2343..2537
                     /locus_tag="IV228_002819"
     CDS             2343..2537
                     /locus_tag="IV228_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718653.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            2623..3294
                     /locus_tag="IV228_002820"
     CDS             2623..3294
                     /locus_tag="IV228_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8718654.1"
                     /translation="MRKWLFCFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
BASE COUNT         1229 a          470 c          739 g         1021 t
ORIGIN      
        1 atttcatttt ttaaaaaagc taaaaacctt gatattactt cttttcaggc aatacgaata
       61 ttattcgctt acattttttt gtataaattt agttgtttta aatttaattg tctgctacgc
      121 ttgtgatgaa ttaattttcg ttctgcactt ttggacgata aatttggaca attgaaaata
      181 caaaaaaaag aaaggccctg tgagactcgg aaggctggta accttcgtcg acgtccacag
      241 gtggcattaa gacaaccttt aatcccttaa tgcgtttacc tttcagtgtt acggtattac
      301 ccgcaacgga tttagtataa cagatattca aaagtgatac cacttttttt ggctgtgtta
      361 aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt
      421 ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat gtttggatgc
      481 gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga aaaataacaa
      541 gaaaaactta ttaaaaatat ttaaagtaag tgggtggatt attttatgtc atatacagga
      601 attttgagtc tcgaagatat ttgtcactac ggtaaacggt gtaccgctac agagaaaatc
      661 acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa atatataatc
      721 cagaaagaca aagtctcaga ggaaatgatt tattacgctg gtaaacaaaa gcaaataatt
      781 ttaaaagatc ctatcccttt aaaagaacta tatccaacaa tcaaacatgt ttatgaccaa
      841 aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc aaaagggatg
      901 gggcgtttaa tcagctgaat tgatcattct cataacttgt attttatcgt ttttcgcttg
      961 cttgaaaata ttgtcgagac ttctaaaaga acttagaatt actagaaaaa taaggagtag
     1021 acttgatcta tcaacaattc ctttcggggg gtgacggggt aggtttttac gattggtaac
     1081 gtaaaaaatt ggtcaatcga gtaaatgccc ttgtttggat agtggtgaaa tgaatttgtg
     1141 taaataatat gcacagagta aacaagaggg aatttagaag ttagacaagt tagaatagcg
     1201 cctagattaa tcatgagcag ctactaaggg atctaggctt tgacgcaaac tcatggaata
     1261 aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta
     1321 catgtagata cttgcgagga cgttggtcat tggatcaatc gagcatttac cgatttctac
     1381 tagggtttct cgtttcggac gccataaacg agtcgatatt ttttatgtat ttttaagtaa
     1441 acgaaaaacg ataaaatatg aacgagtcct gaataaagtg aaaaaattga tataaaacat
     1501 agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga tccgaatata
     1561 cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa aggatttgtg
     1621 acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga cgaggatgat
     1681 ttatactatc aaaaattgac tgataatcca attggattcg gcttgctacc tgaagatgat
     1741 gaaggtaatg aatggtttaa aatgacgctt acgaatgata atggagacga attatctgtt
     1801 gaagatactt ggtcatattt gagtgattat attgtcagcg ttgaaatcat tgaatttgta
     1861 gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc ttagaagaag
     1921 tcacgacgaa taataaaaag gtagtattac cttaggcatt gagtaaggaa ttcatttagg
     1981 aggaaaatta gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag
     2041 gaaggtatgt acagctgtta gctcgtggtt attcacctaa agatgtattc aaatccctgt
     2101 ctaaaggaac agatatggaa aaagaaagat tacgaaaaga attcgattat tggagaaccc
     2161 ataatggaat caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa
     2221 cccaagactg aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa
     2281 ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaat
     2341 aaatggaaga aaagttgcta aaaacgatga aacaaaaaca ccttaaaaga ttatctgtca
     2401 tgcaatatat caatgatatg cagataacag gaaaagagaa agcttgtttg ttaggttcaa
     2461 tgaaaaactt tgaacagttg agacgaactt atgtgaaaac aagttctaat tgtcaactat
     2521 tgttagaagt ctcatgacat tcattaatag aattcatttt taaaactttt tctatggaaa
     2581 tgagaaaaag attttttgtt ttgtctgaaa gggggtagct ggatgagaaa atggttattt
     2641 tgttttttag gagtcgcttt aatcgtcttt tgcagtgtgt ttggctatgt tagctaccaa
     2701 aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa agatgcccgt agatcaaatc
     2761 aatgcacatg caaagagtta taaggaggat gcaacgaacg tcaataatga tatgtctttg
     2821 gggcaaatgt tgtccataca acaagaagca atcgaaatgg gcgtcaataa acaagtattt
     2881 gcacaaattc agattcctgc acttggttta gcattgccaa tttttaaagg ggcaaatcaa
     2941 tacactttga gtcttggagc ggctacttat ttttatgaag atgctgaaat gggcaaagga
     3001 aactatgtcc tagcaggtca taacatggaa atgccaggtg ttttattttc tgatattcaa
     3061 aagttgtcat taggggaggt gatggactta gttagtaacg atggcgtcta tcgttacaag
     3121 gtaacgcgta aatttatcgt acctgagtac tttaaattaa tcgatggcgt tcctgaagag
     3181 aactctttct tgtctttgcc aaagaaagga gagaaaccct tattaacgtt gtttacttgt
     3241 gtttatacgt cacaaggaaa agaaagatac gtggtacagg gagaactaca ataacaaggt
     3301 tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag taaaaaatgg caataaatcg
     3361 gtatgtaaaa aaagctggaa tgcttattgg aatgttagga atcattgcag gctgctttca
     3421 gggatatcga gcatatgcag aagatgtcac gcaaaaaac
//
LOCUS       DACDHL010000171         3363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDHL010000171 DACDHL010000000
VERSION     DACDHL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.175"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..837
                     /locus_tag="IV228_002821"
     CDS             <1..837
                     /locus_tag="IV228_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HAP8718655.1"
                     /translation="KYLEVIVQTSDVEDFFYTIKKYRDELFDWAETYDKIKNFHKQNS
                     TQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKKADPYSDMMKLNELIQEF
                     IEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIEQAN
                     TIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEIDSAA
                     KAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENTEITI
                     QF"
     gene            901..>3363
                     /gene="pglX"
                     /locus_tag="IV228_002822"
     CDS             901..>3363
                     /gene="pglX"
                     /locus_tag="IV228_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HAP8718656.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIF"
BASE COUNT         1323 a          429 c          629 g          982 t
ORIGIN      
        1 aaatatcttg aagtaattgt tcaaacgtca gatgttgaag acttttttta tacaatcaaa
       61 aaatatcgcg atgaattatt cgactgggct gaaacatatg ataaaattaa aaatttccac
      121 aagcaaaata gtactcagaa aaaaatctgg gagaatgcac aagataaaat tagattaatc
      181 gaaaaaagtc ggcgtcaaac ttctagccaa gaaattgatc aattgattca tgagatgaaa
      241 accattttga aaaaggctga tccttattca gatatgatga aactaaatga gttgattcaa
      301 gaatttattg aacaatataa tcaactgtta gagttggaaa gtcaggaaat attaaataaa
      361 ttaacggaag aaaaacaagc aacatttaaa ttattagaag gaaaagattt taaggatcaa
      421 tttgtaccaa tagttgacca agaatttcaa gaactgaaag caaaaattga acaagcgaat
      481 actatagatg atcttgtttt cttatcatta aacgtagcag aacagtattt gatctttaat
      541 caacgtttcg ataatgaaga aaaccgcaga cgtcaagaag aacaagcacg taaacaaatt
      601 gaagcagcaa acttgattcg tgaaactgaa aaagatgaaa tagactctgc agcaaaagct
      661 gaagaaatag cgatagtaaa acctactatt caacagaaaa cagtaccaat gagaacactt
      721 aacgttcatg ttaaaactat taagtcagca gcagatattg atgagttaac agctgaaatt
      781 aatcaaaaac tgaaggaaca acttcaagaa aatacagaaa tcacgattca attctagtga
      841 aaagacaggc ataaaaaggt gcctgtcttt tcaaaggtta catatagaag ggaaagaaaa
      901 atggataaga aagcgataaa gaatttcgcg gtcaatgccc gtagaaaatt aattgatgaa
      961 gtaatgacta aagcaaaacg tttaggaatt gatgaatcaa aaattgaaga tgtgaagaaa
     1021 attgatagca atttacaaga aatcgaaaaa tcaggaataa gaattcaagg aaaagaaata
     1081 gcgcaaagaa acaaattgat tgcagaacta aaacaacgtg cttctgtcac ttcttattca
     1141 gaagcttttc aagagttaat cgaagaggtt gcttatacgt ggtttaaccg attgattgcc
     1201 attcggttta tggaggtaaa tgattattta cctgacacgt atcgtgtatt atctagtgaa
     1261 ataagtggga aaattgagcc agatattatt acagatattt atgatgccga tatctttgaa
     1321 gaattatcta gtaatgaaca gcaacaagtc agaacttgga aaacagataa ttcagcagaa
     1381 gcaatggaca agctgtatca attgattttt ataaaaatct gtaatcaatt gaatgattac
     1441 ttacctgagc tatttgaaga agtagaagat tatacagaac ttttgtttac agcttcttat
     1501 attaaaaaag atggtgtaat cgctgatctt ttaagtattc cagaggatga tttctatgtt
     1561 caagctggtg gacaggtaga gattattggg tggttgtatc agtactataa tagtgagcca
     1621 cataatcaag tggtgaatat ttataaaggg attgtcaaaa aacaagatat tccggcagca
     1681 actcaactat ttaccacaga ttgggtcgtt cgttatatgg ttgacaatag tttaggaaag
     1741 tattggttag atcattatcc caatagtgaa ttaagaaata agttgaaata cttaattcca
     1801 gataaatatg cttttgaaca agcaacatct gtaagtaatt taaaaccaga agaactaaaa
     1861 attatcgata acgcaatggg gtctgggcat attttagttt acgccttcga tgtattgatg
     1921 gaaatttatc ttacgttagg atattctgag cgtgatgctg cacgtgcaat cgttgaatac
     1981 aatttatatg gtcttgaaat cgataaaaga gcgtatcaat tgtcttattt tgcaattatg
     2041 atgaaagggc gtcaatacga tcgtcttttt ttgaaaaaaa gtctatcgcc gaatttaaga
     2101 gagtttattg attctcctaa agtgacagac gagtatcaag agcgtattag tgaattgaac
     2161 aatgataagt atagtgaacg ctggaatgaa tacaaagagt taatttttga tcaatttgaa
     2221 aatgggaaag aacttggatc aatcattaag ttagtcgata aaaataatca ggcaataact
     2281 aaagaatact taggaaaagt aagagagtat atatcaaaat tcaacgaatt tacgaatatg
     2341 gatattcttt atgggatgaa ggaaatacaa gaaaaatact tgcacattct tgacgtggct
     2401 gaaatgctag ttgataaata tgtagcggta gtcactaatc cgccttatct taataaaatg
     2461 gataagaaat taaaaaaata tgtaaatgat aactattctg atgtcaaaaa agatctattt
     2521 tctgtgttta ttaaattgaa tagccaaatg ctgattgata atggatatgc ttcctttatg
     2581 acgccatttg tttggatgtt catttctagc tatgaaaagt tacgctcatt tttaattaat
     2641 gaaagaaaca tttctagttt gattcaaatg gagtattcag cttttgaaga agcaaccgtt
     2701 ccaatttgta cgtttacgat aaaaaattca aatgaacaag atggaagcta tataaaatta
     2761 tcagatttta aaggtggaat ggaagttcaa aaaattaaaa cgttagaagc aattcatcag
     2821 catggtgaat gcgattattt ttatagtact aatcaagaca acttcaaaaa aatcccagga
     2881 atgccgattg cttactgggc aagcgataac gtaatcactg attttgctca aggaaaatta
     2941 actaacgatg ttgcagatgc aaaacaaggg ttggcaaccg gaaataacaa taaatttttg
     3001 cggtattggt ttgaagtaga atttaacaaa ataaagtttg atgctaaaac attagaggac
     3061 gctaaaaagt ctagaaaaaa atggtttcct acaactaaag gtggtcgttt tagaaaatgg
     3121 tatggaaaca atgaatacgt ggttaactgg gagaatgatg gagatgaaat aaaaaattat
     3181 gttaatgaaa atgggaagtt aaaatctaga ccacaaaatc ttaattacta ttttaaaaag
     3241 ggaattactt ggtcgacact ttcatctgga caagtatctt ttagattgca gaatcaggca
     3301 atttttgaaa ctaaaggatc tgttctgttc tcaaaggata taactgactt tgaagaaata
     3361 ttt
//
LOCUS       DACDHL010000172         3363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDHL010000172 DACDHL010000000
VERSION     DACDHL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.176"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(36..443)
                     /locus_tag="IV228_002823"
     CDS             complement(36..443)
                     /locus_tag="IV228_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718657.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(462..1280)
                     /locus_tag="IV228_002824"
     CDS             complement(462..1280)
                     /locus_tag="IV228_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8718658.1"
                     /translation="MDIFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(1506..1799)
                     /locus_tag="IV228_002825"
     CDS             complement(1506..1799)
                     /locus_tag="IV228_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8718659.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFTSNQQRKVKKQID"
     gene            complement(1936..2115)
                     /locus_tag="IV228_002826"
     CDS             complement(1936..2115)
                     /locus_tag="IV228_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718660.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLFI"
     gene            complement(2112..2462)
                     /locus_tag="IV228_002827"
     CDS             complement(2112..2462)
                     /locus_tag="IV228_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718661.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMTTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPDDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(2455..>3363)
                     /locus_tag="IV228_002828"
     CDS             complement(2455..>3363)
                     /locus_tag="IV228_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP8718662.1"
                     /translation="RRKKLAQMIQERIKEELGLIATVGVGDNPLLAKLALDNEAKHNE
                     GFIAEWTYKNVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKSRL
                     GVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQV
                     AIRIRQHNCKTGCVHLDIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEG
                     QEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEKLDKIIDKIRQKYGFTSIVHASSM
                     LEGARSITRSTLVGGHAGGAGGLKND"
BASE COUNT          967 a          638 c          510 g         1248 t
ORIGIN      
        1 acttatactt taattgaata ccgcatgaaa aaaaattatt ttggtaattt ttcaaactct
       61 tcttgtattt cttctcttat gtgttcttct tttgctactc ctagtctatg ctggatatat
      121 gagtctgcta atgtattaac aattttttcg aaagaaattc tccctttttc actttgtaac
      181 tccgtcatat agtcttgtaa aattgagagt ttgctattta atgccggaga gatcttttta
      241 ggaactgata ccttgtttct tttatcagga attgaaactg gtcggcctct ttttttcaat
      301 gtaatatttc ttgtgttact caatgcacta ttgctaaagt tatattcacc gcttattttc
      361 acatcttggc gtttgttatt gggtaataat ggctcaaatt cgccattctc catcatttta
      421 ggaatatcaa aatctttagg cattacttat gcaactcctt attatttatt taaaaactct
      481 tgtctagcat ctagttctgc caaaatattg ataaatactt ggtgtgcttt ataatcccat
      541 tgatcaaaat ttccattttt gtattttctt aaatgtatac caattttaga ataccttctc
      601 aatctttttt gataatgaat gagatttttg gataccactg ttccgtattt ttcaattgct
      661 tcttctagga tttcttcatc aaaatcatca tttggttcta ccatacaagg tacaatatct
      721 ataatttgca aatcaagatc gtatctatcg accatgaatt tttgataatt tacatatttg
      781 ttgactccct ctaaggattc ttcagctgtt tgaaatgaaa ttatcgaata gtctgaagct
      841 gccatagcat tatcggaaaa ttcgctaatg gttggtggta catcaataaa tatttcttga
      901 taattgttct ttattggaag aagtaatttt ttcaagacag taacttggtc taattcattt
      961 tcatgtttat tagcaaaagc ggagaatgat cgaaaactag tgtcacatgc cagaaggtca
     1021 agattttcat ttaccttaat aatactactt gctaattctc catttgcgac accatttgtt
     1081 atagtggttt tgatatcggt tataccgaaa gttcttgcta tgacttgtgt taagttcgct
     1141 tgagggtcta aatcaataat aagagttttt ctatttctta tcattgcatt gtcataagca
     1201 agcatgctga ctatcttagt tttacctact cctcctttaa aattgccaac tgtaaatgta
     1261 gtcccttgaa aaatatccat gtaaatcctc ccaatcttta attgataatt aaagtataac
     1321 ttatatatcg gaaatggtca acattaaaag tataacttaa ataataatat ctaaaaacgt
     1381 ttggatatac cggtttttaa gaataaaagt ataagttata ccatatatat ttagggtata
     1441 acttatactt ttaatattta aggtataact tataccttaa atattgcttt tcgaaaatag
     1501 ctattctagt ctatctgttt ctttactttt cgctgctgat ttgaagtgaa ttgatcatat
     1561 tcattttcta gtattaataa cttttcgtat acatcggtca tattcatact agaagcaaga
     1621 cttagatcat atacaaatga agcaggtaat gattttattc tacgttgtcg ggttatccag
     1681 cttgcaaccg ttccttgtgg gtaggcatat aactcacaaa attgttctac agtgattcct
     1741 aaattcttga taatgtaaac attgatcggg tgcgagtaaa caaatgtatt gatagacatg
     1801 gtgcgtaagc ctactttctt ttattattgc gtttgcaata atagtttaac acacgcatta
     1861 ttctagtatc tactcctatt tgtaaaaaac aaaaaaggat tcatagagat aactctatag
     1921 aatccgtaaa ataccttata taaataacgg gaaaatataa aacctaaaat tatgataacc
     1981 tgctgcagct tctacataga aagagaagcg ctcaacacca acctcattta tcaaacgttg
     2041 gccataaccc caaatctctt ctgatagctg atcaaaggtt atatacttta gctcatattg
     2101 ctggataatg ttcattctaa tcgactccat ttattatcct ggtaaaattc gatatgacgt
     2161 atttcgtcgt agtgaatttt gtctttgcca acgtagatcc ccagctcatc actacctaat
     2221 attttgccaa ttatatcgtc tggatagtat ccttcagcat ttcgttcttc tttttgaata
     2281 catactaatc tattttttat gactgcagcg ttcaatactt ccgaaatctc ttcagtcgtc
     2341 atttgttctt ttgctaagtt ttgattggct cgctgttcat tgtccgcatc gatctcttcc
     2401 gtgtgatcgg ataaatagaa tcctgcccat ttcaatcgtc ctcgatcgtt atagtcaatc
     2461 atttttaagc ccccctgcgc cgcctgcatg gccaccaacc aacgtgcttc tagtaatact
     2521 acgagcgcct tctaacatcg aagaagcatg tacaattgac gtaaaaccat acttctgcct
     2581 aattttatct attattttgt ctagtttttc gtcatcaatc tgtttttgtg gatcactaaa
     2641 caagtccagc tgaagcgaat cagtataaac tagcttgctg taagtgattc cgacgtggcg
     2701 gacttcttgt ccttcataat acttatcaaa caagaaaaga caataattct gcaattcctt
     2761 tgtattgtct gtgataggga ttttcatctg gtgtgagaac cctggtctcg tttctaagat
     2821 cgatgtacca atgtctaaat ggacacatcc tgttttacaa ttatgctgtc tgatacgaat
     2881 ggctacttgt tccgccattt cttttaccac cagctcgatt tcatttcttc tagtataatc
     2941 acgtggcaac acttgtgaat taccatagga cttttctttt gtcggttctg gtgggattgc
     3001 tatatcagtt cgatcaatac catttgcatg aaagtatagc tgtaagccaa tcacacctaa
     3061 acggctctta atggtgtatg gattccagtt agcaagatct ctaatgctca taatccccat
     3121 ctgattgagt cttttcttca ttcgagagcc aatgccccaa aagtccgtca tttcaggaat
     3181 attccatact ttttcaggaa cgtttttgta cgtccattcc gcaataaatc cttcattatg
     3241 ctttgcttca ttgtctaagg ctagtttagc caaaagggga ttatcgccga cgcctaccgt
     3301 agcaatcaaa cctaactctt ctttaatacg ttcttggatc atttgagcga gcttcttcct
     3361 tcg
//
LOCUS       DACDHL010000173         3348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDHL010000173 DACDHL010000000
VERSION     DACDHL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.177"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(133..378)
                     /locus_tag="IV228_002829"
     CDS             complement(133..378)
                     /locus_tag="IV228_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718663.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(372..773)
                     /locus_tag="IV228_002830"
     CDS             complement(372..773)
                     /locus_tag="IV228_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8718664.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(960..2768)
                     /gene="uidA"
                     /locus_tag="IV228_002831"
     CDS             complement(960..2768)
                     /gene="uidA"
                     /locus_tag="IV228_002831"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8718665.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2791..>3348)
                     /locus_tag="IV228_002832"
     CDS             complement(2791..>3348)
                     /locus_tag="IV228_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8718666.1"
                     /translation="PIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAK
                     GFEVEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQ
                     RIVWPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVD
                     KLMGLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         1052 a          650 c          424 g         1222 t
ORIGIN      
        1 aagtgttaac gtactgatag aatctattta attttcaatg aacaataggt tttctaccta
       61 aaagaatcta ctaattcctt aactattaga agattctttt aaacagaaaa agtggaaagg
      121 ggattttttt aattagttaa ttttaaattt taagatcgct tctattaatt taatttctag
      181 ctgacttatc atatattcat ctacataggt aacgaattga cctacctcgt taaatccatt
      241 tgtcaaacat aacttagcca tataagaatg ataatgtttg agtatcatct tgatcgaaat
      301 tggatctcct tctagagcag ataagataat ggatagtgga actaataggt actgccgctt
      361 tttagattta tctaccatag gtcatacccc attttcaatt gttcaactgc cttctttcgt
      421 tgataccaga taccacttgt gctcatattg agaattttac cgatttcagc atcattaaaa
      481 cctgcaaaat aataaagtag gataatcttt cttcttatct ccgataactg atcaattgct
      541 gctgataact tttgatctga aattaatatt tccattccaa ataagaaata gacatcttgt
      601 tcatcaaaat gagtttggga atacctgcct tcatttgaat ctgccaatgg ttcctcatta
      661 tcctttatgt tagcgtttcg ttttcgaata gacttagctt cgtttcgtaa tactttttta
      721 cagtaggcat caaattggtg ttgtatagat atgtcattgt aatgttgttg catgataata
      781 tgctcctttt tccccctttc cactaataca acaatcagaa agcccctttt tttcgaaaaa
      841 actagaaaaa aaacataaaa atatttatca ttctttaaac agaaaatctt tctataaaaa
      901 acaaaaaaaa acgattaggc taggctaatc gttttttgaa cattcttaaa aaatccaaat
      961 tattttttat aactgaaatt gggtattgat gaccatcgtt tctgtagata gtaagcaatc
     1021 attttaggtt ttctatctct agtaaagatt cctttcttat ttccttgcac tcttaaaatc
     1081 ccttgacttg ttgcaaaatc tgcaaaattc cacacttgtt caccaacaaa attttcacat
     1141 gtatctatta cttcgtgatt tgcttcgtaa taagccactt gatactcttc tgtaaacatt
     1201 gttggtactg tatcatgaaa tccagctact gtatctgctc catattctgt aaacattatt
     1261 ggcgtatcag gacatctttc catccatcca tctagctctt ttttcaacag atattttgca
     1321 tgtgccagat cccctcccga aacataccaa ccataataac gatttaaaca taaaacatcg
     1381 acaatatctc ctaccttaca aacatctggc gttgatttta gataagtaac gattgttaca
     1441 ggtcttttct gcggatcaag ttctttagtt aattcaacta aaggttcaaa atattcttta
     1501 gcaccttcgg aatccgaatc tggttcattt gcaatagacc acattacaac acttgggtgg
     1561 ttcttgtcgc gttctatcaa ttccttgatc accgttttat gagaattttc tgttcgtatc
     1621 tctttccacg tattatgttt ttccacatta tcatctaata aagtggccat gaaatttaaa
     1681 tgtaacccta ctgcaggtac ttcatcaata actacaatac cttctcgatc tgctaatcgc
     1741 ataatttctt ctgagtaagg ataatgtgat gttctaaaag aattagcacc tatccatttc
     1801 attaaattaa aatctttaat atttacagcc tcattcaaac cacgtccatt aacatacgaa
     1861 tcctcatgct taccaaatcc tttaaaataa aaaggtttat tattaatcaa aaatttgcca
     1921 ttggtaacct gaacactgcg aataccaaat tcttctgtat aggtatcgat cactttatta
     1981 tcatcaatta aactaatttt catactatat aaataagcgt ctagaggttc ccaaaaaatt
     2041 ggattcttaa gctgaatcat tcctttcttt ccttcagctt cagctacaag ttgagcctct
     2101 tcgtcatata tcgctattaa gatttttccg gtccattctg tatcgatatt gaattctaca
     2161 cttgcatcca aatcagttat tcttgtatta atagtaatat cacttatata gtctttcgga
     2221 gttgtatata acttaacagg tctatgaata cctgcataat tgaagaaatc aaaattaact
     2281 aagttttcct tttttatatc cccattttcc tgtactgttt ccttcataat ccccacaggt
     2341 aaagtactat cgtcaataat attgtttaca gcaactgtaa ttcgattatt cccgtctttt
     2401 acaaaccgat taatttcaga ctcaaatggt gtaaacccac ctttgtgttg agtaataaac
     2461 tttccattca aaaaaacttt tgcttcatgt gttgcagatc caaaacgaag aactaatctt
     2521 tcttctaata atcttttagg aagagtcact tctttttcat accacaccca tccaacatga
     2581 tttctaatgt tctctgtttc aaataaatca ttataagaag agggaactga aatcggataa
     2641 ctctcttttt catttattgg ttctaaagca agttcttcct gtaagtcatc atttgtatct
     2701 aatttaaatc tccagagacc gcttaagtca attacttgcc tcgtctctgt cataatggga
     2761 tacaacatat aaaccactcc tatctaaata ttaaataaat gtatccttta aaccacaata
     2821 agcatctatt ggacttactc ccttaaattc agaaagaccc attagtttgt ccaccaatat
     2881 ttctaaagtt tcttgttttc catcataagt attaatataa gtctttactt gaggtacatc
     2941 agccaaatga aacggacact gtacagatac aaaaattgtt ggaatttcat gaatgtaaaa
     3001 cggtatatcc ggagttcctt tacttgctgg ccagacaatt cgttgaactg tgcctggatt
     3061 aacattagct atattaatga tcaagtcata ttttttagtt aaatttttaa ttggtctctt
     3121 agcagcatat attccagcta tatttttaat tttttcttct tgcgataatc ctttcaattc
     3181 ttcttcaggt gaaatgtatt tttcaacttc aaatcctttt gctgacaaaa tctgtttcaa
     3241 taaatcaatg gcttgaggtc tatttccagc aactacttca ccaaacccac cttttacccc
     3301 atgtacatct actagtaaaa tccttttata tttttctggt acaatagg
//
LOCUS       DACDHL010000174         3250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDHL010000174 DACDHL010000000
VERSION     DACDHL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.178"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2232)
                     /locus_tag="IV228_002833"
     CDS             complement(<1..2232)
                     /locus_tag="IV228_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718667.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3250)
                     /locus_tag="IV228_002834"
                     /pseudo
     CDS             complement(2904..3250)
                     /locus_tag="IV228_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          892 a          725 c          532 g         1101 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg
//
LOCUS       DACDHL010000175         3248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDHL010000175 DACDHL010000000
VERSION     DACDHL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.179"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..206
                     /locus_tag="IV228_002835"
     CDS             <1..206
                     /locus_tag="IV228_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8718668.1"
                     /translation="KVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGY
                     RNQRLAQRILDNELGCANASEAV"
     gene            341..562
                     /locus_tag="IV228_002836"
                     /pseudo
     CDS             341..562
                     /locus_tag="IV228_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            562..909
                     /locus_tag="IV228_002837"
     CDS             562..909
                     /locus_tag="IV228_002837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718669.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            906..1955
                     /locus_tag="IV228_002838"
     CDS             906..1955
                     /locus_tag="IV228_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8718670.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1970..2176
                     /locus_tag="IV228_002839"
     CDS             1970..2176
                     /locus_tag="IV228_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718671.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            2197..2793
                     /locus_tag="IV228_002840"
     CDS             2197..2793
                     /locus_tag="IV228_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HAP8718672.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1158 a          467 c          650 g          973 t
ORIGIN      
        1 tgaaagtaga atttgtctat caatacacat ttgaaacctt acaacaattg gatttggagt
       61 tatttgacta tgtcaattgg tggaaccacc ttcggttgca cggtacactt ggctacgaga
      121 caccggttgg ttaccgtaac cagagattgg cgcagcgaat ccttgataat gagctcggat
      181 gtgctaacgc tagcgaggca gtctaacttt aactgttagc tcctgccgaa gatcgtcaca
      241 tccgaggagg ctcattgtca aggacaatcg gaatagcata cggaaagaag ttctgcacct
      301 tataaaattt gtcaaaaaaa ctgttgccat tccacttaaa acaactgaat ttgatattct
      361 acacgctcta atgtccacac ctaataaagt cttttctcgt agtgatttga tagagaaagt
      421 taagggaata gaatttgatg gactagatag gagcatcgat tctcatataa aaaacttaag
      481 acataaaatt gagaatgatg ctaaatcacc tatgtatatt ttgacagtcc atggtacagg
      541 atacagattt ggtggccact aatgagaaaa agaacgataa aaactcaatt agctgtttcc
      601 tttttagcaa tcgcaacatt aataatagga tcgattagtt tagtcgcatt aagtttgatg
      661 aacaatcatt ttagtaaata cgtggaagag cggcaagaag atttgttgaa tcagtatgta
      721 tatacaattg atttattgtg gttaaatagt ggggaaacat ggaatagtga agaacttgca
      781 gcactatctg agaaagtatt agagaataat atttattttt ctatagaaga cgaacaaggc
      841 aacatggttt gggaacttac tggaaaagat ttgaagtcag cacaggagaa actgaaaaaa
      901 atgcattgaa agtttctgag aaaaacagtg tgaaactaga cgaaacaata gaagtgaaaa
      961 agaagttaat taatgatgga aacgagtttg gaaaagtaac gttttattat ttcggacctt
     1021 ttgcttatac agagcacgat gccttgttta tctccagtat gaaacaaagt ttaatgtatg
     1081 ttgcaattgc agctcttctt gtatcattca tacttgcttc gtggatctct gctagattag
     1141 gattgccact aaagcatgta agcgatttta cacataaatt aactcgtggg gaatatgctg
     1201 ataaaatccc acaagaaact tcaattattg aaataaattc cttaattgat tccctaaatg
     1261 atttaagtaa tcagttggag aaacagcatg ggcttcgaaa aagattaact accgatattt
     1321 cacatgaatt aagaactcca ttagctacgc ttaaaggcaa tgtggaagga atgattgacg
     1381 gagtctggaa aataactccc gagcgcctac aatcttgtta tgacgaaata gatcgtctaa
     1441 ctcgtttaat tggaaatata gaaataatca acaaaattga agcaaaatat gatcatttaa
     1501 ataaaactga gtttaacata tataagttga ttgaatccgt tattgaaaat ttcgccagca
     1561 aaattgagtc aaaaaacctt catgttgaaa tccaagggga taacatcaat atatctgcag
     1621 ataaagataa aatgaaccaa gttgttatca atcttttgaa taatgctatt aaatttacgc
     1681 aaaaggaggg aaccattaag ttttctataa gtaaaaataa agaccatgtt cttttaattg
     1741 ttgaagacaa cggcatcggt atagaaaaag atcagcaact tcatattttt gatagatttt
     1801 atatggctga cccctccaga agtagagctt taggggggca aggtatcgga ttagctattg
     1861 taaagagcgt tattgaagcg cataaaggta gtataactgt aaaaagtaaa ttaggtctag
     1921 gaactaaatt tgttataaag ttaccatttc aataaaggag gagagttatt tgtttcaaaa
     1981 acagataaaa attgctaaac tagtatgtga atgttgggca aaaaaaatat tttttcaact
     2041 gatttcgata gatagtgtcg aaaaagttca tttagatatc ttatcaaatt ctgtagtaat
     2101 cttttctaag aaggacttat caatagctac aattaaaaaa gaagtttttg gattacaaat
     2161 ttatgagatg acctaaatgt gattaattaa aggaggatga agacagtgaa aaagatgaca
     2221 aaattagtat ttgcaagtct tggaattatt gtatttttag gagcgtgtag caatcaaagt
     2281 gaatcgaata attctaatag cacaaacgag gaatcgactt ctacagcgtc tagtgaaatg
     2341 aatagtatgg agggaatgaa tcacgaaggt atggttcctt cttcaatgaa agatgctgcg
     2401 aaccctaaat tcccggtggg aagtaatgtt atactgctag gcgatcatat ggaagggatg
     2461 aaaggtgcaa atgctcaagt tgtaggggcc tttgatacta caatgtatga agtaagctat
     2521 gagccaaaaa ctgggggacc aatggttaac aatcatcgat gggtagtaca agaagagtta
     2581 aaagatactg agactgtagc aaatgagggc gatactgtta tcttgaatgc cgatcacatg
     2641 gatgggatga tgggagctga agcaaaagta gataaatcta ttacaggaac tgtttacgtt
     2701 gttaattaca ctccaaccga tggtcaagaa gaagttaaga atcatatgtg ggtaactgaa
     2761 gatgagatgg aatatgacga aaataatgag taattttata tactaaacca acaagctggt
     2821 acatggtctt cgtattctca acatgaggaa tatgagctct ttgttaataa ggactgatga
     2881 gttgtgcaaa atcaaatctg ggtcagaacg aacctcattc tggctcagat ttttggctct
     2941 tcgacattta gtgctggtag agagacaact gaaaatttag tcataaccag ttaggcagag
     3001 tgcttaactg gtttttgttc gcatttatta ttcttttgat attggctgct tgcccttcaa
     3061 ttttatttgg tttcttaatc aactcttcat ataaccccaa atcatatatt aatgaggtgc
     3121 ctcctattcc tataaatcta tctcttcttt aatagtatga gaaaagtcta ctcttgtaga
     3181 tttaaattgt caacaaaaaa tttacagatg taaatgataa gttgaataga gaaagataaa
     3241 ttattttt
//
LOCUS       DACDHL010000176         3223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDHL010000176 DACDHL010000000
VERSION     DACDHL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.180"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(554..2167)
                     /locus_tag="IV228_002841"
     CDS             complement(554..2167)
                     /locus_tag="IV228_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8718673.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="IV228_002842"
     CDS             complement(2308..3216)
                     /locus_tag="IV228_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8718674.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          554 c          572 g         1059 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctt
//
LOCUS       DACDHL010000177         3219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDHL010000177 DACDHL010000000
VERSION     DACDHL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.181"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(144..1088)
                     /locus_tag="IV228_002843"
     CDS             complement(144..1088)
                     /locus_tag="IV228_002843"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8718675.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1384..2328)
                     /locus_tag="IV228_002844"
     CDS             complement(1384..2328)
                     /locus_tag="IV228_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718676.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2549..>3219
                     /locus_tag="IV228_002845"
     CDS             2549..>3219
                     /locus_tag="IV228_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8718677.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVS"
BASE COUNT          931 a          622 c          519 g         1147 t
ORIGIN      
        1 catatgtacg gatacatttt cggaccgatt aattttaacc attctgttta tttgcttacc
       61 tattagatat caataaaaaa ctgcttaaca gctatttcag ctgttaagca gttttaggat
      121 ttttagttca tattcattgt ttcttataat acagtgttca atacttctct taaagcactt
      181 gttgatttgc tgaacattgc tttttcttct tcgttgattg ataattcaac aacttcacga
      241 acaccatttt cgtcaacgat tgatggcaca cctgtaaaga tatctttttc gtcgtattca
      301 ccagttaaat aagcagatac aggcaataca gcttgttcgt tattcaagat agcttttacg
      361 atacgtgcag tactcatacc aataccatag taagttgctt ttttacgatc aatgatctca
      421 taagcagcat tacggacttt atcagcaatc acatctaatt catcttgggc aatacgatgg
      481 tctttttcta caatttcaaa cactggttta ccaccgacag ttgtatgtga ccatgcagca
      541 acttctgaat caccatgttc acctaagata tagccgtgga cgctgcgtgg gtcaactttt
      601 aatttcaatg cgatttcttt acggaaacga gttgtatcca atgttgttcc agtaccgata
      661 acacgtgatg ttggcagacc agattcgttc caagcgatat atgtcaaaat atctactggg
      721 tttgaagcaa caacgataat accgtcgaat cctgatccca tgatttcttt tacgatttgg
      781 cgcatgattg acgcatttgt tttaactaaa tctaaacgag attgaccagg tttttggttg
      841 ataccagctg tgataacaac gatatcagca tctttacatt cttcatagcc acctgaccat
      901 acagccacat tttcatcgcc ccaagccata ccatctaaaa ggtccaatgc ttccccttct
      961 gctttttctt gattcacatc gatcaatact aattcattag agattccttg gttgatcatt
     1021 gcataagcga tacttgttcc aacaaaacct gtaccgacga ttactacttt acgacttgtt
     1081 tttttcatga ataaacagct ccttttcata actatttgtt actttcatga aaagtatagt
     1141 cttgtttatt cattttatca acatgttaat tataatagtt gttattataa tttttgatgg
     1201 aaacgaattc ctgaatacgc ataacagata aatgacaaat gtttacacct atatcccctg
     1261 ataatacaac caaaagttta gaactgaaaa taaatccaaa aaaattcgct gcaacgatat
     1321 aataagacag caatcgcacc taaaacaatt ttccttcata tgctttagtg aaagattttc
     1381 ttcttaatgt ttatttctcc attcattggg agtacatgtt gaatacttct tgaataattt
     1441 gaataaagaa gtcgtttgtt tatatccgat acgattagcg atttcttcca acgaaaaact
     1501 cgtttcttga atcagttcca acgcaaagtc gaattttttc tttagaacca gttcttgaaa
     1561 ggaagcatca aaatacattt tcaatttatt actaagatag accggattgt agccaaaatg
     1621 agctgctgtt tcttttaacg tacaagaagt aaaatgctta tcgatataat ctaatactct
     1681 aagcatctca ttttctgcgc atccatctgc tgatgaaaag ctttgttgtt ctaataaatg
     1741 agtcaattcg atcagtaata gtgatgtata gagatttaac gaacggatct tatgactgta
     1801 tgtatcaaaa taaatcaaca gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc
     1861 gccgcgaaaa tgaatgaatt gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa
     1921 agaagaaagt agattatttg tgcttgccaa atgattcaat atctctgtag ataaggtttc
     1981 ttcttttaat aagatgttga ttaaaatatc ctcttgccct gtgtattcga ttctttgagt
     2041 agcttcttta tcaatgatca ccacgtcact ttcttccatc tctatccttt ctccattaat
     2101 aatttgtaaa catgtaccgc tatacatata attgatttca acaaaatcat gtgtatgctc
     2161 gggaacttcc gcatatcgac ggtgtttact gatatgaata tttccctgtt gaaatagatc
     2221 ccctcggtgg atttttggcg gtgtctgaag atggtaagct aagtttaaat cattgatatt
     2281 cattccttgt tcacgctgca gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta
     2341 attcagctag ttccaattga ctccctcctt tttgtaagcg gtttctttcg cttattagtt
     2401 cacgctgtac tgctattata tgcaaagcta tttttggttt cataaagact ttctatggtt
     2461 gggacatgaa cttcctgtga ttccgctatt gagagaataa ccacttcata ctatattttt
     2521 gataagcgct tacaaaaatg aaaggaacat gcttatgaat aaaaagatga tgaaattttc
     2581 tctgctgtca gtctctctta ttttgacttc tgctggtgca gtcacgtctg caattcccag
     2641 tatgtcagct acttatgcca cgcgttcttt atctgctgtt gaattattaa cgactgctcc
     2701 ctcttttatg gtcactattt ttgttttgat tagtagtttt attgctagga aaatcggaca
     2761 aaaacaaact gtgatcatcg gattagtgat tgcatcgatt tatggtatcc tccccctctt
     2821 ctcacaaagt tacagtattg tcttagcttc aagagctgga ctaggtattg gctttggatt
     2881 gatcaattct ttggcagtca gcatgattgc tgccttttat cacggagatg aacgagcctc
     2941 tatgattggt tttcaaagtg catttcaagg atttggtgca gctttaatga catttgttgc
     3001 aggacagcta gtacagttcg gttggcaata taccttttta gtctacgcca tcagcttacc
     3061 tattcttgtc ttgtttgttt tgtttgttcc tacacctgaa acaactgaac ttcaatcaca
     3121 tggcacagcc ccaacaagac aagtaatcaa aacgaatcct aaaatgatta gttatggtat
     3181 ttttctcttg ctggtcatcg tggtatataa tgctgtatc
//
LOCUS       DACDHL010000178         3168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDHL010000178 DACDHL010000000
VERSION     DACDHL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.182"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            165..2777
                     /locus_tag="IV228_002846"
     CDS             165..2777
                     /locus_tag="IV228_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8718678.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            2774..>3168
                     /locus_tag="IV228_002847"
     CDS             2774..>3168
                     /locus_tag="IV228_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718679.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMA"
BASE COUNT         1134 a          488 c          606 g          940 t
ORIGIN      
        1 gaacaacaat tagaagaatt attgaatcgt accaatcagt caacaacaga gtcaagcatg
       61 gaaaatagca aagagagtga ttctagtaag gctaaagaag gatcaagcga ttcaactgta
      121 caatcaaaca cttccgaaag ttcagaatca aatgaggtga aataatgcgt gttagagaaa
      181 caacgaagcg aaaagacaaa cgattcaatt tagaaaagaa taggtatctt tctcatttct
      241 atcgctatac tccctcagga gaagagctac cagaaaaaca tgacaaacgc atttcgaaaa
      301 agatttttta tagtggactt gtatacctcc ctttagagta tttagtaaca agagtaccag
      361 aagttttcaa ccgattggaa attgttaagg gaagcttaca aggaataaat ctgtttcaat
      421 tacaggaaat gccacaggtc tttcattttg attcagctgg tttttggaat ccatttactc
      481 ctacaactag tttgggtaca cgtttagcaa tttcgacagt ttgtgtggtt ccagctgtac
      541 ttatgggatt gaaaatggac atgtggtttc gtcctctagc agatggacaa aaaggggata
      601 atcgattaat gaccttaaaa gaaatccaac agatttatcc gcaaatacct gaaacaaaaa
      661 ctacttttcc aggttatgga ggtattccaa tcacacatta caaaaaatat tggtacattc
      721 aaacagatac tgttaatacg tgtattgttg gtacctctcg atcagggaaa ggtcagattg
      781 aagtgttagc caccattgac aatttatctc gtgcagaaaa acaaagttca atggtcgtta
      841 atgatccaaa aacagaatta tatgttggta gtaaagatga actagaagct agagggtatg
      901 atgtatatgc ctttaatatc accgatccac tgcaatcaat gagtgataat ccattggcac
      961 tcatcgtgaa atactggaaa cgaggagaca tagatactgc aacacagtta accaatactt
     1021 tcacaaatac gatttattat gatgccaacg cttcagataa caaatacttt aacgaaaacg
     1081 cgcagaaagc tgtcaatgcg cttatttttg cgttgttaga acaagctgat caaacaggag
     1141 attactccaa attgacaccg aataatctcg ttgagcttct ttctgagatt ggcggtttta
     1201 attattatcc agatccagac aatgaattca aacagttgaa tgccttagat gaatttatga
     1261 atcagttgcc acctggaaac attgcaaaaa aacaatatgg ggcaacgaaa attggatctg
     1321 ataaagcgaa gggaaatatt ttatcaacgg caatcacggg attgaatcca tttactctaa
     1381 cgaaaattgc aaagatgact tctcaatcaa cgattgatta taaatcggtt gggtttccaa
     1441 aatacttaga tttgaaatta catgaaagtc ttttaaatca acgagtcgta gtagaatttt
     1501 atcataaggg aaagaaattt catgaagaac gtgtcaaagt aggctataaa ggattttgtg
     1561 aaatcaattt tgattgtcat ttagttgcag gtgatcaagt ttgtttaaaa tacgatcatc
     1621 aaggaaaaga atatgtggct tggtatgaat tttctcaaag agtattgaaa gacgaagagg
     1681 gtaatatcgt ttatcggaaa aaacgaggag aaacccattt gccagaatta gaaaaagaag
     1741 ccgttctaac attagatgag gagaaaagta acttattagt agagtctatt cggatgcact
     1801 ataccgataa accaatggct atttttatgc ttacacctga ttatgatcca tctaaccata
     1861 tcattgttag tatttttctt agtcagttat ataaagaatt atctacgcaa tgtgttaaga
     1921 ctaaaggaga taaatgtcac cgcaggattc attttatttt agatgaattt gggaatatgc
     1981 caccaatgga taatatggaa ggaatcatga cggtcacagc tggccgaaac atgttgtggg
     2041 atctgtttat tcaatcttat cagcaacttt atgcgaagta tggagaacaa aacgcagcga
     2101 ttatcaaaga aaattgtcag acacatattt atattatgtc gacagatgat aataccatcg
     2161 aagaattttc taaaaaagtg gggaataaga cggttgaaca agagaacagt accatcaatt
     2221 cattaggtat gaatactcat atcaatcgca atgttgattc agatcgtatc ttaactcctg
     2281 aaagaatttc tactttttta gaaggtgaaa cgattgtact cgctccttta aaacgacaag
     2341 atttgaaagg tcataaaata cggccgtatc ctatttttaa tcatggggaa acgaatcttc
     2401 cttatgctta tcaatatatt gaagatttca atccatcgtg ggatatcaat gatatagaca
     2461 tacattgtac acatgcggac ttggatctta aggctttaca aattgattat gattatttcc
     2521 taccagatga atatgctaga aatctctatc atggactgca tgatcaatta gaagaaaaaa
     2581 acgaagaaaa tatgttttat aaaatcgttc aatctcttga gaatgagacg gctaaaaaat
     2641 tgttaattca attttacgaa gaacaagcag cagatcgact tcaagaatat gttgaaaagt
     2701 tgatcaaaaa taaagaaatt tctgaaatga ttgggaaacg gttgttattg gaatctgaaa
     2761 aggtggtgaa agaatgatca ttggaggaat tttttctggc atagccgact ggtggtcagg
     2821 cttattcgga gaaggggatg caaaccccaa aaccgtaacg aaagtcttac aagattatgg
     2881 taattatctg cagtatcaag atgtatttag ttggatttgg aacacttttc tgtggggatt
     2941 gattaaagga ttatataatc tcaatacttt actagaaaaa accatttatc aaagctttga
     3001 tttaaaagac ttattgaacg ccgctggggt aaatgcacta tttcaaagtt tgattagtaa
     3061 ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttgaaat tttttatgag
     3121 taaacaccca ccaaaagtca aaaatatctt ggttaattta tttatggc
//
LOCUS       DACDHL010000179         3148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDHL010000179 DACDHL010000000
VERSION     DACDHL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.183"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(126..773)
                     /locus_tag="IV228_002848"
     CDS             complement(126..773)
                     /locus_tag="IV228_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8718680.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1181..1684)
                     /locus_tag="IV228_002849"
     CDS             complement(1181..1684)
                     /locus_tag="IV228_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8718681.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1807..2571)
                     /locus_tag="IV228_002850"
     CDS             complement(1807..2571)
                     /locus_tag="IV228_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8718682.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2679..2954
                     /locus_tag="IV228_002851"
     CDS             2679..2954
                     /locus_tag="IV228_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8718683.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT          881 a          653 c          556 g         1058 t
ORIGIN      
        1 aaaggggctg tccccagact ttagtaggcg tcattagcta tctttcgtta gcatttaaag
       61 gcgaacacca tcacctattc acttccatta aaagatagca tatttttctg taaaaaacaa
      121 taaaattatc gttctttctt tacacgttca gcaaattcat agaaaggagc caatttcgtt
      181 aactgttgat tacattggtc actatcatga caaatataat tccccttttt cgtatacgtg
      241 ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaaa cattcgatgt tttctgacag
      301 attgcacaga ttcccttatt tatcgttggt gataaagtac cactaatccc ttttaatttt
      361 tcatcttcgt aatagatcat gaattttttc tgcgtacctg gatcattcca gccgatgtac
      421 gtatgttctt gcaagtcgat ttcattccaa gctggttgtt tgagtttttt cacttttcgg
      481 aataatttct cgatttgtgg tttgctaggc tgcataaacg gctgaacgat ttgcttcagc
      541 tcctccaatg ctcgttctgc ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa
      601 aatgtatcga tttctggcag ttcgtgttct aatatgtctc gaatcttttc agttgctagt
      661 gcctgaactg tttgtaccgt ttttggatca ttgacagatg agtacgcatt taataactgt
      721 tctacttctc tttttataaa aaaatattgg tatggtttta ttgtttgttc catgatttta
      781 aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa aagtgatctt ctgcaaagta
      841 tgtgttacct gaagatcccg gactctttta aataagatac gttttgcatc ttatcatcac
      901 cccctttaat gcttattctt ctatgtacat gattcattcg ataaataaaa gtaaaccgta
      961 tcttgaacag atagtcaact gcaaacagaa aaaaggactg tgacaaaagt cgtgtcaaag
     1021 tcccttgttt cgaatgaatg gtgttcaaaa gcttgcatct gcggtaataa gccgaaactt
     1081 cacaaaaatt tgagaagtaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
     1141 ctgtcacaac ctcaatatgg tctattattg gttttacctg ttaaatcgag gattctttcc
     1201 caaagtaacg ttgccatctc ttacgcatac ttgcagacat gggctgagcc gcttctttga
     1261 ctgcgcagta tttgtccacg aatgtcacaa tatatccttc tttatactta ggcggtgcga
     1321 tcgttgcacc ccacatgtgc ttcaaaatga tatctcgctc aagatccgac aaatcggtga
     1381 ttttttctgc attctttaca gcaattcttg gatggatata cgcatgtgtc ccctcgtcaa
     1441 atttcgtcgt acgccaatca tagtagaaca gatcatgcaa aagtccagca cgcgctgttg
     1501 ctttagcatt gccaccccat ttttttgcga gcagataact atagtaagaa acactgatgg
     1561 aatgctccaa acgagtggaa tgaacatgct gtgtgtaatt cgctaatttt ttcactgctt
     1621 ctgtttctaa aagatcttgt acataagaca tatattcttc gtcttcacgc catttttctg
     1681 tcataattat cgatctgcca cctttttgaa tttcattttc ttctgaaatt gatctaagta
     1741 taccaccagt gttcggatta ttctagtcgc tctagataaa atagctaaag cttaatagtt
     1801 ccttaatcat aaagaaaaat gataaagaac gatccctgct gcgactccga cattcaatga
     1861 ttccgcattt cctttcattg ggatatatac gttctgatca gtcaaagaca agatttcctg
     1921 acttactccc tgaccttcgt ttcccatgat tatagcgaaa tcctctgttt tttctacttt
     1981 gtctaatgca tgtgcttctt catttaattc tgtaccgtac accgtaaacc ctttgccttt
     2041 cattacaggg atcacttctg ccaaatcttt ttgtaacaca ggcaaatgat aattgcttcc
     2101 ctgcatgctt cgcagcacct ttgtactata aatatctgcc gttcctttac ctaataaaac
     2161 accagccata ccaaacgcat ctgccgtacg gatcatcgtt cccacatttc caggatcctg
     2221 tacattatct agtaaaagcc agcctcctga aaaatctggc gtatcttcag atgttaacgt
     2281 catcaccgct aagatccctt gcggtgtagg tagatctgac atagcggtca tgacctcatc
     2341 tgaaactaga aaagtagaaa tctctttgtt tacagccagc cagtctcccc attctttcat
     2401 acctctctgt gtcacaaaaa gttcttttat ttctgcatca gacgacgcag cttcttcgat
     2461 cagatggaac ccttccaaaa gatattctgc cttttcttct ctgtatttct ttttatgtag
     2521 cttttttaat tctttgattt ttggattttt tgctgattgg atctctttca cattccttca
     2581 cctcacctga ttatatcatg gaataggtgc ttgagcttgt ttcatcctaa aaaaatcggt
     2641 atcattgatt tataaaacag gaaagaaagg tgacgaacat gcgcaaagtt aaaatgaatg
     2701 tccaaggacg tgtccagggc gtgggatttc gctatatgac caagatggtt gctgatcagt
     2761 taggcgtgac cggaacggta aaaaacgaag acgacggctc agtcacaatc gaagcaatcg
     2821 gaaatgacga catcatccaa aaatttatcg aagaagtcaa gaagtctccg agcccaagtg
     2881 gacgagtgca gtatgtggat gtccaagaag atccgatgat tgaggaacga aagaaatttg
     2941 atgtggtggg ataaggttgt gaaaaggtcg cttagttttg agtattaagg aaaattttgt
     3001 gaaaatggct ttccacattt ttgcataaat ttgacttaat accgagaaac tgacttttga
     3061 acaccgtcga tcaggttgtt tttgaggcgc tttgcctcaa gtattaaaga taggtagtaa
     3121 aaaagctgat ctataacttg caataaat
//
LOCUS       DACDHL010000180         3010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDHL010000180 DACDHL010000000
VERSION     DACDHL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.184"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            62..1150
                     /locus_tag="IV228_002852"
     CDS             62..1150
                     /locus_tag="IV228_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718684.1"
                     /translation="MYSKNWKKKLVMGVMVGSIFVFAGCSASTDKSKTATSETKTAEV
                     VKSKMKQIEKNVEKILAEKDKDTKLSEYKKFVTESSAYSKSDKTEKKITNTYTESIKK
                     IKTNFKEQDDKDLKENTLDNLEEATENDLSQKVNTLDELKKWVQKESTDVYSQAEVDS
                     LIKKIDDTITTYKNQLEKIKIDNQEATSTETTTESSEVATLQENVSEEPQVVHTNLLN
                     SIEEAAQYLGQKLPQSGGSDAFENGQQNSDGSFTFHYQLHGGPEGITWNIITVNNNGE
                     IIQDDYFKTVPNEATNNNVSTLSQAAISELEAAQLAIAKHNELGRPNATDAGSETTAD
                     GYYFYYMADGTDQMYKYFVSNSGQVQAE"
     gene            complement(1607..1774)
                     /locus_tag="IV228_002853"
     CDS             complement(1607..1774)
                     /locus_tag="IV228_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718685.1"
                     /translation="MSIREIVRIQSFPDSYIFVGSSLSAKYKVIGNAVLPKLAKVIGD
                     SIVKQLSESNF"
     gene            1819..2241
                     /locus_tag="IV228_002854"
                     /pseudo
     CDS             1819..2241
                     /locus_tag="IV228_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752995.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1160 a          434 c          489 g          927 t
ORIGIN      
        1 aatataacca cttttatgaa aagatgttat aattaactaa aaaataatag ggagaaaaca
       61 catgtactca aagaattgga aaaaaaagtt agtgatgggt gttatggtag ggtctatttt
      121 tgtttttgca ggttgttcag cttcgacaga caaaagtaaa acggctactt cagaaacaaa
      181 aactgctgaa gtagtaaaaa gtaaaatgaa acaaatagaa aaaaatgtag agaaaatttt
      241 agccgaaaaa gataaagata caaaactgtc ggagtacaaa aaatttgtta ctgaatccag
      301 tgcttactct aagtcagata aaacggagaa aaaaattact aatacctata cagaatctat
      361 taagaagatc aaaacaaatt ttaaagaaca agacgataaa gacttaaaag agaatacact
      421 agataatctt gaagaagcaa ctgaaaatga tctttctcaa aaagtaaata cattagatga
      481 actcaaaaaa tgggtacaaa aagagagtac tgatgtatat agccaagcgg aagtagattc
      541 attaatcaaa aaaattgacg atacaattac cacatataaa aaccaactag agaaaataaa
      601 aattgataat caggaggcta catcaacaga gactacaaca gaaagtagtg aggtagctac
      661 tttacaagaa aatgtttccg aagaaccaca agtggtccac acgaatttat taaattctat
      721 tgaagaagca gctcaatatc tgggacaaaa acttccacaa tcgggcggtt cagatgcatt
      781 tgaaaatggg caacaaaatt ctgatggctc gtttactttt cattatcaac tacatggagg
      841 tccagaaggt atcacttgga atatcattac agtgaataat aatggtgaga ttatccaaga
      901 tgattatttc aaaactgtac ccaatgaagc aacaaacaat aatgtctcta cacttagtca
      961 agcagctatt tcagagttag aagctgctca acttgcaata gctaaacaca atgagttagg
     1021 tagacctaat gcaactgatg ccggaagcga aactacagca gacggatatt atttttatta
     1081 tatggccgat ggaactgatc agatgtataa atattttgta agtaattctg gtcaagttca
     1141 agcagaataa cattgagtta taatgatatt atcagatgaa ctgtatcata aatttttaac
     1201 gtgaaatgtt tgtaatatca attggagaat ggtatatgga tttactaaaa gataataact
     1261 gttatttttt agtaaattca tactatttag aaaagaataa tttgctgaat acttgtaaac
     1321 tatgtgtgat acttatctta tatggatata gactcgtggt gctagttaat ttcagcttat
     1381 tataaaccaa cattttcatt ttataaaaac gactaaaaac agaatttctg agtaaaattt
     1441 caaaagtaaa aatagtgata ttatgctctt gttcctagat tcaataatta actagcacca
     1501 cgagtttata aaattggcaa taaaatttta atcattatta acataacctg gatctgtaaa
     1561 gcaatttaac tcttaataaa aaagcatcta aaaaaagcgt gttcagctaa aagtttgatt
     1621 cagaaagttg tttaactata gaatcaccaa ttacttttgc aagttttggt agaacagcat
     1681 taccaattac tttatatttt gctgaaagag agctaccgac aaaaatataa ctatctggaa
     1741 atgactgaat tctcactatc tctcttatcg acactcttag atgaactcaa aaaaatgggt
     1801 acaaaaagag aatactgatg tatatagtca actagaaata tcgctcatag agtgcgtgtt
     1861 cgaggtcgaa aagaaatctg tgaggagttc aaattgattt accatgcgaa ttcaaaagaa
     1921 gaggcattga attacatcga ttttatgatt aggaaatgga aaaagcagga tcgaagagtc
     1981 gtgaacctac tcttgaatcc tgccctatta accttttata atttccctta cgctatcaga
     2041 cgaacaattt attcaacgaa cctgattgaa ggctttaata agcagctaaa acgatatact
     2101 cgaagaaaag aacaattccc taatgaagaa tctctctaga gattcttagc ttcgcaattc
     2161 aactaatata accagaaatt tttaagtaga gttcacaaaa gatttaaaga aattcaagat
     2221 acattagagt cgatgattta actgtaatta acagaatgga ttttccattt acacataatt
     2281 cttgacgcta cctcataata attaagagct ttaaaattag tatttattag atacttaaaa
     2341 gctattttag tattgataaa ttgttaccag caacaaaaaa gaagttactt agtgctaact
     2401 tttgagcaac tgctagagga ggtaggatat ggctaattat cctgtaattt ttattgtatt
     2461 ttctagaaat gtaattattc tcttaaacat tccagcatat ccaagtatat agaaaattta
     2521 cgttttctat atagttggat gtttacaagt tatatctggt ttaataagtt tttttctgtt
     2581 agtcatggca tgttttattg tgctggttca tcaatatatt gaaatgtact aaaatgaaat
     2641 tttcagttat ggtataatat ttatgctgtc tgctaccccg gcctattatg gcaaggggag
     2701 gaggttgtat tatgaattac cttttcgata tgttttttgc aatcttagca gaagtatccg
     2761 cgcattacat ctgtaaatgg cttgattgca aaattattgc caaaaaagac agtaagccta
     2821 cccgttaatc tgaatagaac gtaaaagaaa accactagcc cgccagctag tggtttttgg
     2881 tgtatcatga attacctttt cgttaaaaca attataccat atttcctaaa aaatacaatt
     2941 aatagacgaa ttatcattac cactttgatg aaaaaagctg acataaattt ttcttttatt
     3001 tttataatga
//
LOCUS       DACDHL010000181         2985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDHL010000181 DACDHL010000000
VERSION     DACDHL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.185"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            687..>2985
                     /gene="esp"
                     /locus_tag="IV228_002855"
     CDS             687..>2985
                     /gene="esp"
                     /locus_tag="IV228_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8718686.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPA"
BASE COUNT         1091 a          441 c          576 g          877 t
ORIGIN      
        1 tctaactttt tcagaaaagt taaacaacta caagttaata gattataagt atttgagtga
       61 aagcaattaa ttattcatgt agagtactag acactttaaa tatggacaat tcaattataa
      121 agaaaaagaa tcaataattt aatgaggctg tgacaaatgt cattttttgt ttcgctaaaa
      181 acgagtgaaa aaatgttgat aactccaatc attcgtttaa gattctagga atcattcagg
      241 aatgctgtga aaccgatact ttataattat aaagaaggtg atttcaaatt ccgaagaagg
      301 aatttgaagt caccttttta cttatgtcac agtctcattc agtaatgcgg gataaggctc
      361 cttattccgc tatttgaata ctctgaagtt taatctaaga aagagtaata ttttgaaaac
      421 atgcttcatc gcatagaaat gaacttaatt tcagaaagaa tacatacata tctttttata
      481 tgttttcagc tgctgaatgg ttaaaaagca caaatatccg aaaaaaacat gaagaaatgt
      541 actttttttg ttgtaatgtg catgaaattc tttaagtaaa ctagttaaca taagtttagt
      601 aagattccta ttctgtgaat aatacgtatc agatttatct tttattctta ctaagaaaat
      661 aactattaag ggagttgatt tgaataatgg ttagcaagaa taataagaga gtatttctag
      721 aaaaaacaaa gaaacgagta ttgaagtaca gtatcaaaaa attaagtgtt ggggttgcat
      781 ctgttttggt tggtgttggt cttgtcttgg gaacaactga actagttcaa gcacaagatg
      841 aaatttctcc aagcacccca cttgaaacag cgataagctc cgtacaagta ggtgacaaag
      901 tagcttcggg gaatactttt caggagaatc ctggttatac gaaaaattat aacttttcgg
      961 atttacaatt tagtcctcaa gaactaactg gagacacact aaaaggcaat acaattggat
     1021 ttgaagttta cggaaaacat aatattgcag catcaactaa aaactgggaa atccgtcttc
     1081 aattagatga acgattagct aaatatgtag aaaaaattca ggtcgatcca aagaaaggaa
     1141 ttggatcttc tagacggacc tttgtaagaa ttaatgattc gctgggtaga cctacaaata
     1201 tttggaaggt caattatatt cgtgcaagtg atggattatt tgctggggct gaaacaactg
     1261 atacacaaac tgctcctaat ggggtaatta cgtttgaaaa gagcttagat gaaattttta
     1321 aagagatagg catagacaat ctcaagaccg atcgtttaat gtatcgaatc tacttggtaa
     1381 gtcatcaaga cgatgataaa attgtaccag gtattgatag tacgggctac tttttaacag
     1441 attcagatga tttctataat agcttagatg tatctgaaaa taatcctgac cagtttaaac
     1501 atggatcagt aagtgcaaaa tatgaggaac ctaatactca aacaaaagat ggttcaggat
     1561 ccacaggtgc taatggagct ataatcttag atcataagtt aacgaaaaat tataatttct
     1621 catattcagc ttccgcaaag ggcacgcctt ggtatgctaa ttacaaaatt gatgaacgat
     1681 tagttccata tgtagcgggt atacagatgc acatggttca ggctgacaaa gtaacatatg
     1741 atgtttcttt tgaatcaggg aaaaaagtgg ctgatttagc aattgaacgg cgcaaagatc
     1801 atgaaaatta tggtgtaggt tcaattactg ataatgatct taccaaactt attgattttg
     1861 ctaatgcaag tccacgtcca gtcgttattc gatatgtttt acaattgact aagccattag
     1921 atgaaatctt agaagatatg aaagcaacag cacaagttga agaaaataaa ccatttggtg
     1981 aagatttcat ctttgattct tggttgtcgg atacgaataa aaaattaatc cagaacactt
     2041 atggaacagg ttattattat ttgcaagata ttgatggtga tggaaaccct gacgataaag
     2101 aagagagcgg agacacgaat ccatatatcg ggaaacctga attagaagaa gtatatgatg
     2161 ttgacacaac agttaagggg aaagtattca tccacgagtt agcgggaaca ggtcacaaag
     2221 cccaacttgt tgataaagaa ggtactgtat tagcagaaaa aactatcgct ccaaatgaaa
     2281 aagatggggc tccaatttca gatactgtag aatttgaatt tacgggtgta gattcaagta
     2341 aattaatcgc gaaagatgaa ttaaaaatcc aaatcgtttc tccaggtttt gataaaccag
     2401 aagaaggttc aaccgttatt aaggaatcac caaaagcggt tgataaacaa accgtggtag
     2461 ttggatttaa accagatgct aaagaatcaa ttcggaataa taaaaactta cctgaagatg
     2521 cagagtattc atggaaaaca gagcctgata cttctaacgt tactgatagt acgaaaggta
     2581 ttgtaactgt taagatcgga aatcgaactt tcgacgtgga tgtagagttt gctgtaaaag
     2641 cttctcaagc tatggaaaat gatgcaacat acgtacctat aacaacaact ccagaaacga
     2701 cagttcaaag tggaaaacct acatttgata aaccagatgt tcctttagct aaagatgcct
     2761 tttcaatttt agatgtctat aataaggact tcggtaatgc aagtgttgat gcaaatactg
     2821 gtattgttac attcactcca gctaaaggtg taggagaatc ggagccgatt actggaacaa
     2881 ttcctattaa aattgtttac caagatggtt ctgtaggcac gaccgattta gcagtaactg
     2941 taagtaaaga tatttatgaa aatccaggag aaaacattcc tgcag
//
LOCUS       DACDHL010000182         2792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDHL010000182 DACDHL010000000
VERSION     DACDHL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.186"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..1086
                     /locus_tag="IV228_002856"
     CDS             4..1086
                     /locus_tag="IV228_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8718687.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1304..2446)
                     /locus_tag="IV228_002857"
     CDS             complement(1304..2446)
                     /locus_tag="IV228_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8718688.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2451..2636)
                     /locus_tag="IV228_002858"
     CDS             complement(2451..2636)
                     /locus_tag="IV228_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8718689.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          925 a          512 c          439 g          916 t
ORIGIN      
        1 gatatcaatg atgatggctc aagaaaaaaa gtggagaaag ttggcggaga cacccgacca
       61 gaggccgaag ctgctttacg aaaagtttta tcagatattg acgaaacagg acaatacttt
      121 ttaggtacgg atactcgagt aaaacaatac cttgattttt ggatggagga atacgttaaa
      181 ctaaatctaa aatacaatac ctatgaaaac taccgattta ccatcaaaaa tcatataaac
      241 ggttatttag gaaagaaaaa acttacggat ctctcccctg ctcttttaca aaatttcatc
      301 aatgctgaat ttaaaaaggg ttactcgaag aaaacaatga ctattactca ctctgtcctt
      361 aagaatgcgc tgaatatggc ggtttatcct tggggattaa tcaagcaaaa tcctatgctg
      421 tatgtaaaaa taccaaaata tgaagcacga ccaacgacta aaaaagatct aaaaatcatt
      481 tctcttgagg actttgatca tatgctagaa attactcctg aaggacatcc tttctatatt
      541 cctttgaata ttggatttta tacgggaatg cgcgttggcg aagtttgtgg tctgacgtgg
      601 gatgatgtcg atttttcaaa tggaacaatt actgtagaga aacaaatggt aaagaatgac
      661 ggcgcatggg tatatggtac gccaaagaca agcagttcca atcgaacgat ttttattgga
      721 caaaccttgc tagcaattct gaaaaaacat aagaaacaac aattagaaaa tcgaatgaag
      781 tatggaaagc tctacattga ttcaaatgca gtatgtacga aggaagacgg tgagctagtt
      841 acgccaagtg tagtgaaatg gaatactagg aggatatcga atgcactctc cctctctttt
      901 aacttccatt ctctcagaca tactcatgct acacttcttc tcgaaaatgg cgcaaaaatg
      961 aaagaaatct ctgaacgatt gggccacagc agaatttcaa ttacgatgga tacttactcg
     1021 catgtaacag ataagatgag aaatgaaacg gtcgatatca tggaaaatct tagaaagaat
     1081 tcttgatttt tgccaccgaa aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa
     1141 catggtttat tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc
     1201 gtccattttc gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa
     1261 aaataggcta tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg
     1321 taatttcatt tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag
     1381 tagttttaat agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt
     1441 caattaaaaa ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg
     1501 caatttcagc tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat
     1561 caaaagaaaa aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt
     1621 ttaaatcttc aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg
     1681 tttcagcaaa ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc
     1741 tgccatttat taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac
     1801 ctgtaacaaa aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat
     1861 aaacactatt atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct
     1921 gagtttttat tttacctata gtatctacag gattttcttc aataatttca agtacaattg
     1981 cccttttaaa aactactctc aacttattga atatctgttt aatataattt ggtgataatg
     2041 gttgctcaat gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta
     2101 tatgaattgg tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta
     2161 aaatatgatc tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa
     2221 aatactctcg ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag
     2281 aaaaactttg ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc
     2341 ttctggtcac ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc
     2401 aagtgcctct ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc
     2461 caagaaatat ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta
     2521 cgataaaaaa agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta
     2581 gcatagtact gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata
     2641 gcaccttcct tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg
     2701 aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta
     2761 aatcatcgct ttttattgac tataaaaatt at
//
LOCUS       DACDHL010000183         2787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDHL010000183 DACDHL010000000
VERSION     DACDHL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.187"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1184..1510
                     /locus_tag="IV228_002859"
     CDS             1184..1510
                     /locus_tag="IV228_002859"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8718690.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1518..2426
                     /locus_tag="IV228_002860"
     CDS             1518..2426
                     /locus_tag="IV228_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8718691.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
BASE COUNT          855 a          578 c          490 g          864 t
ORIGIN      
        1 tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt tccagtttta
       61 ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat tctcctcttt
      121 atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa aagcatttct
      181 aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt taactatttg
      241 atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga cctttcggga
      301 gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt tcgtatacct
      361 aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa aacaaggggc
      421 ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc cttgttttgt
      481 cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac gaaaatgacc
      541 tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct cccaaaatgt
      601 cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt aagtattaaa
      661 taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca aaaataaact
      721 gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta atttatcgat
      781 tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca gcgaatccag
      841 cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc ttcctacgaa
      901 aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta ttccgtgtta
      961 ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg gcggggctgt
     1021 atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat caatgaatgg
     1081 cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc gccggatgac
     1141 cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca aaatctttcg
     1201 taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta tcggaatcac
     1261 agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta caaaaggtct
     1321 caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt tggaatcttc
     1381 cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg tcacactggc
     1441 tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag atgcaaaaat
     1501 ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct ggtcttgaaa
     1561 tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg aaaacaagtg
     1621 aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat gatgttttta
     1681 gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa catcccggtg
     1741 aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt gaagaaatcc
     1801 aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc caatgcctca
     1861 accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt caagctcttg
     1921 cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta gacactcagc
     1981 caataacagc gattgcgatt ctttcggaaa taggaccgga tatgtccgtg tttccaactt
     2041 cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt gcccgacgaa
     2101 caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt gtacagattg
     2161 ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat cgtagaatca
     2221 aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc ctgaccgcta
     2281 tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg gagccaaaac
     2341 ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta agaaaacgcg
     2401 gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct aaatagactc
     2461 gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac cgttcttcag
     2521 gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact ctcctatagc
     2581 ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc cgtaataagc
     2641 ctcctagtat ataggcagac tgccaattat aactgattca atccacatta tgataaaatc
     2701 caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac gagtcttttc
     2761 attcagtgct ttgtaaatac tcaaaat
//
LOCUS       DACDHL010000184         2726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDHL010000184 DACDHL010000000
VERSION     DACDHL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.190"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            243..1202
                     /locus_tag="IV228_002861"
     CDS             243..1202
                     /locus_tag="IV228_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8718692.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1189..1794)
                     /locus_tag="IV228_002862"
     CDS             complement(1189..1794)
                     /locus_tag="IV228_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718693.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2034..>2726)
                     /gene="tnpB"
                     /locus_tag="IV228_002863"
     CDS             complement(2034..>2726)
                     /gene="tnpB"
                     /locus_tag="IV228_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8718694.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          878 a          481 c          435 g          932 t
ORIGIN      
        1 ttttttgtat ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg
       61 tatcgaggca gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca
      121 cttgtcacat ttttttattt ttttaccttc gccgattgta aaattaagct agacaactaa
      181 aaaagtcatt tgtgctacac tcaaaatagt tccgtcgaaa gaattataag gagtgagtac
      241 aaatgaccta tacccatctt acaccaactg aactcgtaat gatagaagct tatttcaatc
      301 aatcacactc tgtttcaaaa gtagcccatc tggtacaacg ttctcgacaa accatttata
      361 atgtctatcg ttttcttaaa tctggtggaa gcgcaataag ctactatgaa caatataaga
      421 aaaacaaacg taactgcgga agacgtccga ttgtgttacc agatgggcaa caagaataca
      481 ttcaaaaaaa ggtggctcaa gggtggactc cagatgtcct tattggtcga gcagagtttc
      541 ctattgcttg ttcggtacgt acactatatc ggatgtttaa agataaaacc tttgaccctc
      601 acgatttacc aatgaaaggg aaaagaaaac caaacggaca taaagaaaaa agaggaaaac
      661 aagcttttcg ccgttcgatt caggatagaa caactgatta tatacagttc aatcatgagt
      721 ttggtcacct agaaggtgac accattgtcg gtggaaaaca taaaagtgcg gtgatcacgt
      781 tagttgagcg attatctaaa gtgatcatca ccttgaaacc cgctggtagg caagctatag
      841 atattgagaa tagactgaat caatggtttc aatctgtacc gaaacacttg tttaaatcca
      901 tcacatttga ttgtggaaaa gagttctcga attggaaaaa gattagtaat accaatgata
      961 tttcgattta tttcgctgat ccaggaacac cttctcaacg aggactaaat gaacattcga
     1021 atggcttatt acgtaaagat ggcttatata aagctatgga ttttaataag gtatccgaaa
     1081 cctttatcca atcggtggca tcaaaacgaa atcatattcc tagaaaatca ttacactatc
     1141 gcacaccatt ggaagtattt ttgagttacg taggtaaaga tgatttgtct agcttaattt
     1201 gacaaacgaa actttgtaaa aatattcgat tgaatattct atttggcacg actcacttgg
     1261 cagtatatgt atgtcaatat ttttcatctg tttcataggt atttcaaaat ctaaactatt
     1321 aaaaacatat aattccctgt aattatttac atctaactct actaaattta tctttggaac
     1381 aatagatcct ccacccattc caccataata aaaaaaatct tgttctctat caaaatagca
     1441 catattaagc tcagtttgtt tatcatcctc aataatttta taaatcaaac ctatataatc
     1501 atgaatgctg gcaaaaattt tttttaagtc aagttctaaa tcttcttcga aaacacattt
     1561 aatttctcct actgataatt gatactctat attacatgac ttaattagtt ttttagatcc
     1621 cccctccaga taggcatatg tttcaataat tatgttcttt ttattgcttg gcaaatttcc
     1681 gttatttatt gcataaatta aaaatgactg tctatttagc ccataatcta atacaacttc
     1741 ttcatactca tatttcttta aatatttaat atgaaaatat gctgaattaa tcaaaactcg
     1801 agtgttactg ttgttattta ttgttgttct attgtgtata aaattgtata ggttgtcata
     1861 ttttcttggt ttagtgaact actcaccact tagctaaacc taacggtttc ttacgtttga
     1921 agtgggagct tcttgggaag agagcatact tgatagcgta ttactttact aacagaggtg
     1981 cctcttattt accaagctat ccccgtagtt cctacggttc ttgtttattt gatctaagcc
     2041 aatcccatag agtgtagcct tagaccttcg gtcaggatat tgatactagc gttgatatct
     2101 cggtcatgat gagtatgaca aatagggcaa gtccattttc gaatttcgag agatttcttg
     2161 ccgtctttat gtccacaatt cgaacagatt tggctagatg gaaaccactt atctactttg
     2221 atgattttcc gaccgtacca gtcagccttg tattgtaatt tagacacaaa actagaccaa
     2281 gaaacatccg aaatactttt tgctagttta tgattacgca acatgccttt tgtgtttaag
     2341 tcttcaatac agatgatatc gtgatttttg ataatttctg tactcaactt attcagaaaa
     2401 tcagtacgtt gattcattac tttttcatgc aatcgtgcta ctttacgttt ttgtgtttga
     2461 taatttctag cttcaaacag attgatacct ttttgtttag ctaataaggc acgtcttgac
     2521 aacttacgtt gttcacgttt gagtttcttt tccattttgg acgtaaattt attattatca
     2581 aatttttgac cggtagaaag aattgcaaag tctataatac ctaggtcaat accaacagca
     2641 gaattagatt taggtagttc gcgaacttct tctttgcata acaaagaaat ataatattta
     2701 ccgctagaat gacgtgatat tgtagc
//
LOCUS       DACDHL010000185         2712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDHL010000185 DACDHL010000000
VERSION     DACDHL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.191"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..287
                     /locus_tag="IV228_002864"
     CDS             <1..287
                     /locus_tag="IV228_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718695.1"
                     /translation="VFRDKARLGTMYPYIVYSNVSNSKKMASGKVYKKLPYYQVSFFT
                     LGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYYTYVRCVENAR"
     gene            277..642
                     /locus_tag="IV228_002865"
     CDS             277..642
                     /locus_tag="IV228_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8718696.1"
                     /translation="MPGNKNGFSEMADYLGNLSRVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMSEQIKVVVDDDRVKVQFEETAFYWRFVENGATNRRAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            649..1275
                     /locus_tag="IV228_002866"
     CDS             649..1275
                     /locus_tag="IV228_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8718697.1"
                     /translation="MGKQDTLYFEGLDDILIAMMATPDTVGTAPTYSEVVRLPIGTKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIEVMDEIKGLVARSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANFVSI
                     GLKYNNVIYASFDSNREEATMDLFEKFIAQPVYDEEQWKTLAKVGGAG"
     gene            1275..1739
                     /locus_tag="IV228_002867"
     CDS             1275..1739
                     /locus_tag="IV228_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718698.1"
                     /translation="MARLSDYGINVEDLKNSATVTIQGVEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLYKKEGKISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            1934..>2712
                     /locus_tag="IV228_002868"
     CDS             1934..>2712
                     /locus_tag="IV228_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8718699.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQA"
BASE COUNT          966 a          438 c          584 g          724 t
ORIGIN      
        1 cagtgttcag agacaaagcc agattaggga cgatgtatcc atacattgtg tactcaaatg
       61 tgagtaacag caaaaaaatg gcatccggta aagtctataa aaaattacct tattaccaag
      121 tctctttttt tacacttgga acagaacagg atttagttgt tattgaacaa gcgctgcaaa
      181 atgctggcat tccatattca gactttacag gtatacaagg tgatgagaat gacgataccg
      241 tgaccaacta ttacacatat gtgaggtgtg tggaaaatgc caggtaataa gaatggattt
      301 tcggagatgg ctgattattt gggtaatctt tcgagagttg atcccaaaaa attatccttg
      361 gaatccttag aagaagcagc taacttttat ctaaagcagt tgcttcccaa tattcctaag
      421 tccctgctta agaaaaaaca tatgagcgaa caaataaaag ttgtggtcga tgatgatcga
      481 gtgaaggtcc agtttgaaga aactgctttt tattggcgat ttgttgaaaa tggagcaact
      541 aaccgaagag cccaacactt tgcaagtggg acatatgaac aaaacaaaga aaaaatagaa
      601 gagattatga caaagaaaat acttgattta tgggaagggt gattttaatt gggaaaacaa
      661 gatactttat attttgaagg cctcgacgac attttaattg cgatgatggc aacgcctgat
      721 accgttggga ctgcgccaac ttatagtgaa gttgttcgtt tgcctatcgg tacaaaaatc
      781 ggcgtcaaag gtaatggtac agcgttagag aaatgggcat caagtaaaat gttccgacgt
      841 gtatcgcgag aaacaaaaca tgaaattgcg ttggatcatg tgggtattcc gattgaagta
      901 atggatgaaa taaaaggatt agttgctaga agcggagtaa cgtttagcaa aaataccgca
      961 cgagaatttc cgtattttgc ctttggattc atcggaaata ttgagaatgg cggtaaaaaa
     1021 gcggtatggt atcctaacac acaactatct aatgtaattg atgaagagta tgccactgca
     1081 gaagaagaga caaaaatcga cgatgtaact gcaaattttg tttctattgg actgaaatac
     1141 aataatgtaa tatatgcaag ttttgattcg aatcgcgaag aagcgacaat ggatctattt
     1201 gaaaaattca ttgctcaacc tgtatacgat gaagaacagt ggaaaacatt ggctaaagtt
     1261 ggaggtgcag gctaatggct cggttatctg attatggaat taacgttgaa gacttaaaaa
     1321 attctgctac tgtcactatt caaggtgtag aattccctat ctcatttact atgcaaacaa
     1381 tggaatttat agcagatgta tatggtggag attattcgca attcgaatct gatatgaatg
     1441 ccatgctata caaaaaagaa ggaaaaattt cttctgctaa cttatcgcct agtgacttaa
     1501 aaatcatgcg tgccttgatt tatgcaatgt tgcgcactgg tggtttagag gaagatccag
     1561 aaactatttt taaattctta ggaatgagtg gagaggtgtt gtctgcttat agtacctgta
     1621 tggaaatttt tgctagccag acatttcagg tggaagacct aaaaaaatcc aagaagccac
     1681 aagactttca aaaaacgcaa gcaaaaagaa aggtaaacaa aaagaatcgg aagagatagg
     1741 aactccttgg agtttttata tttacgttgc tctcactcta ttgaattgga gtgagagttt
     1801 ctttttgaag tctacaccta acttgtggct caaatcttat ttacagtggt tacaacaaaa
     1861 caccgatttt gaaccacctc aatctgtaac tatggataaa tcgccttggt ggtagaaagg
     1921 agcgctaaga taaatggctg gtaaagaatc tgatgtcgtt cttaatttta aaacgaatgg
     1981 cgaagtcagt tattcgaaaa caatcaaaga aatcaacaaa gaaatgaact tagccgctgc
     2041 cgagtacaaa aaccaagtgt ctgcgatgga caaggatgca actcaaacag aaaaattgcg
     2101 agcggctaaa caaaagttag aaaaacaact ggggttagct gaacaacgat ctcaaatgtt
     2161 gagagaagaa tatgaaaaat ctgtcaaaga gacaggcgaa tattctgctg aatcagaaaa
     2221 actttataaa caattactca attctgaaac cggagaaaat aaacttcgta cagcattaga
     2281 acagacgaac gatgcactta aagaacaagg tgacgtttct gttgatacag caaaaaaact
     2341 ccagaaaatc gaagaaacag gtgagaaagt aaaaggtgtt ggtgaaaaaa tgtctgttgg
     2401 agtaaccgcg cctattgtag cggcaggagc agcaggactt gcagcatttg gtgaagttga
     2461 cgaggcactt gataccatca ttacaaaaac cggagcaaca ggtgatcaag ctgatagact
     2521 ttcacagtct ttcaaaaacg ttggttcaaa tactcattta cctttacaaa cggttgggga
     2581 agctattggt gaggtaaata cacaatttgg attcatggat aaaaaactgg aagattcaac
     2641 caattatctc ctacagtacg ctgaaatcaa tgatacagat gtttcgcaat cagcaatatc
     2701 tgctcgacaa gc
//
LOCUS       DACDHL010000186         2712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDHL010000186 DACDHL010000000
VERSION     DACDHL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.192"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..287
                     /locus_tag="IV228_002869"
     CDS             <1..287
                     /locus_tag="IV228_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718700.1"
                     /translation="VFRDKARLGTMYPYIVYSNVSNSKKMASGKVYKKLPYYQVSFFT
                     LGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYYTYVRCVENAK"
     gene            277..642
                     /locus_tag="IV228_002870"
     CDS             277..642
                     /locus_tag="IV228_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8718701.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            649..1275
                     /locus_tag="IV228_002871"
     CDS             649..1275
                     /locus_tag="IV228_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8718702.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            1275..1739
                     /locus_tag="IV228_002872"
     CDS             1275..1739
                     /locus_tag="IV228_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718703.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            1934..>2712
                     /locus_tag="IV228_002873"
     CDS             1934..>2712
                     /locus_tag="IV228_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8718704.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQA"
BASE COUNT          971 a          442 c          571 g          728 t
ORIGIN      
        1 cagtgttcag agacaaagcc agattaggga cgatgtatcc atacattgtg tactcaaatg
       61 tgagtaacag caaaaaaatg gcatccggta aagtctataa aaaattacct tattaccaag
      121 tctctttttt tacacttgga acagaacagg atttagttgt tattgaacaa gcgctgcaaa
      181 atgctggcat tccatattca gattttacag gtatacaagg tgatgagaac gacgataccg
      241 taaccaacta ttacacatat gtgaggtgtg tggaaaatgc caagtaataa gaatggtttt
      301 tctgaaatat cggattattt aggaaacctt tcaaaagttg atccgaaaaa attatcgttg
      361 gaatctttag aagaagcagc taacttttat ctaaagcagt tgcttcctaa tattcctaag
      421 tcgctcttaa aaaagaaaca tatgcgcgat caaataaaag tagtagttga aaacgatcaa
      481 gtgaaagttc aatttgaaga gacggccttt tactggcgct ttgccgaaaa tggcacaaca
      541 aatcaacgtg cccaacattt tgcaagtggt acgtatgaac aaaacaaaga aaaaattgaa
      601 gaaattatga ctagaaaaat acttgattta tgggaagggt gattttaatt gggaaaacaa
      661 gatacttttt attttgaagg attggacgac attttaattg cgatgatggc aacgccagat
      721 tctgttggaa ctgcaccgac ttatagtgaa gttttacgat tgccaattgc cacaaaaatc
      781 ggtgtcaaag ggaatggaac agcattagaa aaatgggcat ccagcaaaat gtttcgtcgt
      841 gtgtcgcgag aaacaaaaca tgaacttgcg ttggatcacg tgggtattcc gatagaagtg
      901 atggatgaaa tcaaaggatt agttgctaaa agcggagtaa cgtttagcaa aaataccgca
      961 cgagaatttc cgtacttcgc ctttggattt attggaaata ttgaaaatgg cggtaaaaaa
     1021 gcagtttggt atcctaatac acaactatcc aatgtgattg acgaagagta tgccactgca
     1081 gaggaagaaa caaaaattga cgatgtaact gcaaatctgg tttccatcgg actgaaatac
     1141 aataatgtga tgtatgcaag ttttgattca aatagagacg gagcttctct agaattattt
     1201 aataaattca ttgctcaacc tgtatacgat gaagaacagt ggaaaacatt ggctaaagtt
     1261 ggaggtgcgg gttaatggct cggttatctg attatggaat tcacgttgaa gacttaaaaa
     1321 attctgctgt tgtaattatt caaggcgcag aattccctat ttcatttact atgcaaacga
     1381 tggaatttat agcagatgtg tatggtggag attattcgca atttgaatct gatatgaacg
     1441 ccatgctatc taaaaaagaa ggacaaattt cttctgctaa cttatcgcct agtgatctaa
     1501 aaatcatgcg tgccttgatt tatgccatgc tgcgtactgg tggtttagag gaagatccag
     1561 aaactatttt caaatttttg ggaatgagtg gagaggtgtt gtctgcttat agtacctgta
     1621 tggaaatttt tgctagccag acatttcagg tggaagacct aaaaaaatcc aagaagccac
     1681 aagactttca aaaagcgcaa gcaaaaagaa agaaaaacaa aaagaatcgg aagagatagg
     1741 aactccttgg agtttttata tttacgttgc tctcactcta ttgaattgga gtgagagttt
     1801 ctttttaaag tctacaccta acttgtggct caaatcttat ttacagtggt tacaacaaaa
     1861 caccgatttt gaaccacctc aatctgtaac tatggataaa tcgccttggt ggtagaaagg
     1921 agcgctaaga taaatggctg gtaaagaatc tgatgtcgtt cttaatttta aaacgaatgg
     1981 cgaagtcagt tattcgaaaa caatcaaaga aatcaacaaa gaaatgaact tagccgctgc
     2041 cgagtacaaa aaccaagtgt ctgcgatgga caaggatgca actcaaacag aaaaattgcg
     2101 agcggctaaa caaaaattag aaaaacaatt aggattggct gaacaacgat ctcaaatgct
     2161 gagagaggaa tatgaaaaat ctgtcaaaga aaccggtgaa tattctgctg aatcagaaaa
     2221 actttataaa caattactca attctgaaac cggagaaaat aaacttcgta cagcattaga
     2281 acagacgaac gatgcactta aagaacaagg tgacgtttct gttgatacag caaaaaaact
     2341 ccagaaaatc gaagaaacag gtgagaaagt aaaaggcgtt ggtgaaaaaa tgtctgttgg
     2401 agtaaccgcg cctattgtag cggcaggagc agcaggactt gcagcatttg gtgaagttga
     2461 cgaggcactt gataccatca ttacaaaaac cggagcaaca ggtgatcaag ctgatagact
     2521 ttcacagtct ttcaaaaacg ttggttcaaa tactcattta cctttacaaa cggttgggga
     2581 agctattggt gaggtaaata cacaatttgg attcatggat aaaaaactgg aagattcaac
     2641 caattatctc ctacagtacg ctgaaatcaa tgatacagat gtttcgcaat cagcaatatc
     2701 tgctcgacaa gc
//
LOCUS       DACDHL010000187         2672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDHL010000187 DACDHL010000000
VERSION     DACDHL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.193"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..391
                     /locus_tag="IV228_002874"
     CDS             14..391
                     /locus_tag="IV228_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718705.1"
                     /translation="MDVLDEIIATHAEVKNYSEAVRFLCMNYDSEVEKKVNSNQAKLN
                     AMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVEGKIKANQTKKYSPKI
                     KKTISESVTTKSEVFDPTRFLKR"
     gene            411..2000
                     /locus_tag="IV228_002875"
     CDS             411..2000
                     /locus_tag="IV228_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718706.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2012..2347
                     /locus_tag="IV228_002876"
     CDS             2012..2347
                     /locus_tag="IV228_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718707.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2488..>2672
                     /locus_tag="IV228_002877"
     CDS             2488..>2672
                     /locus_tag="IV228_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718708.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1060 a          407 c          545 g          660 t
ORIGIN      
        1 ttatgaaaca catatagatg ttctagatga aattattgca actcatgcag aggttaaaaa
       61 ctatagtgaa gccgttcggt ttctatgtat gaattatgat tcagaagtgg agaaaaaagt
      121 aaattccaat caagcaaagt tgaatgcaat gggaaaagaa atttctattt taaccgaaat
      181 ggtttcagag tttgcggatc tccatttgaa agatacaggg attttaattg gtacaaattc
      241 aactgtttac caagaagcaa aagagcgtgt ggaaggcaag ataaaagcaa atcaaacgaa
      301 gaaatattct cctaagataa agaaaacaat aagcgaatca gtgactacaa aaagtgaagt
      361 atttgatcca acaaggttct taaaacgata aaaattagaa ggagaatgtg atgagtaaat
      421 cacctgcagt cattctcact tgtcaattta ccttgcccaa taaaaaatct ttttcaacat
      481 acattgatta tatgacaaga gaaaaagcgc tggaagaaaa ggaacgacgt acgcccgagg
      541 aagaacagga actgcgtcta atcaagaatg ctttagagga tttttatatt cctgaaggag
      601 aaactttttc ggagagtaag acgggtaaag aattcagcga aacgtcgaat gaagcgcaat
      661 cgttgatgaa acatggaatc aattttgaca agttagaaga acaagatttt accaaatatc
      721 tttcttatat gacacgtcac tatgcgttag aacaaaagaa agtactgacg catagtgaac
      781 aaaaagaata ccaacgcttg aaccaagcaa taaaaaaata tcaaacagag ccacacaaaa
      841 aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg aaaggaagtg
      901 atctgaagga gatcaagcaa accttccaaa gaggacaaca aaaaggctca atcatttatc
      961 aagatgttgt ctctcatgat cattcgtatc ttgaaaaaat aggattgtac gatcccaaaa
     1021 cagatacttt agatgaagaa ggtttaaaag cagctggaag aaaaatgatg gagacattgt
     1081 tcgagaaaga gcaactgaat gaaacaggct actggatggc aacgattcac cgaaatacca
     1141 aacatatcca tattcatttt gcagttgttg aaaaaaaaaa tacgcgtaaa ccatacactg
     1201 agattgaaaa tggtgtgagt tatatcgtac cgaaagggaa gagaaggcaa gcaacattag
     1261 acgaaatgaa gactaattat gtacatgagt tagaacgttt ttcttatgaa aaaaatcgaa
     1321 tgggtatgga gaaacgaaat ttattgacac ggaaaagtga tttgcgtaac accttaacca
     1381 aagtagtgaa agagccagta cgttatgatc gacacgcaat gagcttatta actgaagtat
     1441 ataaaaactt acctaaaaag cgttcggaat ggcattacgg ggatgaaaat cgaaccaaac
     1501 tttcaccata cacaagagag aaactcgatg agttaaccca atatgtgtta acaaaagaac
     1561 ctggatatca agaatatata cagatttcaa aagcattgaa agaagaagca aaaagtcttt
     1621 atggggaaag caagagagaa tcaaaggatg cagaaaaaaa tgctctcttt gatttaaaaa
     1681 agcgcacagg aaatgccatt ctaacagaac tgaagaaaca ggatagtcaa ataaaacctt
     1741 taatagaaag gatggatcag atgcaatcta tttacaatga acgatccttt cgaacactta
     1801 aacagttgcc aacgaatgaa tacaaatata gttacgaaga attcatgaag cgcaggaaac
     1861 aattccaaca aagagttgtc acgcaacgga gtttgcgtca attaaatcga acgattaatg
     1921 cccatgaaga aaaataccac gcaagtaaag catacgaaga gcaacaacga agaattcaaa
     1981 tggaacaaga aagacagtag gaggaatagc aatgaaacaa tatttagatc aatggaaagt
     2041 tatagaagga tcattaagag aagaacgaat cgaacaactt cccgattgtt tagaaaaaga
     2101 gcatttgttt cagataagag aaatgttgat aaacgaacag tttgatccaa atcaattttt
     2161 agtcgttgag tacccagcta ctggtgtgta ctgttgtaat catgtgaagg gtgaaaaata
     2221 ttttatcatt caagaatatg agggcaagtt agcgccttat tatactactt gggaaatgaa
     2281 tgaagagggg atcaataact tcccgtgtaa aagcattgaa gagtctattt cactcacaga
     2341 atgttagttg ttaagtgaat aaaagaagta aaagagtcct gaacgtgatt gtccaggact
     2401 cttttttttc actgaattgt tttccgccaa ttcattgagt ggtgcgattt ccgtctacat
     2461 tatatcaaaa gaaaggagga agaagaaatg gcttattcaa cgattggagt tgttgttgaa
     2521 aaaagcagag acaatcttgt ttttgtgaca gaaattcaaa caggcagagc ttttgtagtc
     2581 acagacaaag cggcaaaagc atatcaaagt ggcgatattt taacgttaaa tctgactacg
     2641 aagacgttcg tcgatgcggc agaagattat cc
//
LOCUS       DACDHL010000188         2505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDHL010000188 DACDHL010000000
VERSION     DACDHL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.195"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1372
                     /locus_tag="IV228_002878"
     CDS             <1..1372
                     /locus_tag="IV228_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8718709.1"
                     /translation="KNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRASKE
                     TFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLVDY
                     DIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNK
                     ILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSAAQ
                     ELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYN
                     LIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSL
                     GISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSA
                     SSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKE
                     LLIDNN"
     gene            complement(1658..>2505)
                     /locus_tag="IV228_002879"
     CDS             complement(1658..>2505)
                     /locus_tag="IV228_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8718710.1"
                     /translation="KQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKG
                     KKELLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQCTI
                     SKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKY
                     RGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQL
                     LSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMFLWLFR
                     MGVIP"
BASE COUNT          892 a          378 c          450 g          785 t
ORIGIN      
        1 aaaaaatgca tttgcttatg taactagaaa aagtttaaaa tcattaatta tcatattagt
       61 tattttatct atgtcaactt taagtctcat tagtttatct attaaagatg ctacggacag
      121 agcttcaaaa gaaacatttg ctaatataac aaatagtttt tctatggaga taaatagaca
      181 agtaaatcca ggaacaccta gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat
      241 atctcaaaca gatagtatag actcctatgt aaaaagaata aacagtgttg cagatttagt
      301 tgattatgat attattgaaa ctcaagagac tcttgcgaat caatcacctg aaagagcaaa
      361 gaattttaaa agaacagtta tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt
      421 tgtatcagaa gcatataaat tagtagaagg aaagcattta gaaaatgaag ataaaaataa
      481 aatcttaatg cataaagatt tagctgaaaa aaataacctt aaagttggag ataagataaa
      541 aataaaatct aatttatttg atgctgataa tgagaaaggt gcagatgaaa cagtagaagt
      601 agaaattaaa ggtctattcg atgggcataa tagcggtgga gtaagtgcag cacaagaact
      661 atacgaaaat acattaatca ctgatattca tacagctgct aaagtttatg gtaataccga
      721 agatacagct gtataccaag atgcaacatt ctttgtaaaa ggtgataaga atcttgatag
      781 tgtaataaaa gatcttggga aattagatat aaattggaga gaatataatt tgattaaaag
      841 ttcatcaaat taccctgcat tacaacaatc tatatcaggt atttactcaa tttcaaataa
      901 attatttttt ggctcattaa tatttgcagg agttgtagtt tcattattat tattcttatg
      961 gatgaatgct agaaagaaag aaatagcagt attactatca ttaggtatat caaaattaga
     1021 aatttttggt caattcctaa ttgagatggt atttatatca atccccgcat tcgttggttc
     1081 ctatttctta gctcaatata cagctgacaa gcttgggaat aatatattga ataaggttac
     1141 tggtgatata gctaaacaaa tagctaaaca atctgcatct agccaattag gtggtggggc
     1201 agaagctgaa ggatttaaca agacattatc aagtttagat atcaatgtat tacctaaatt
     1261 catgatttat gtagttatat ttatgagttt agtacttctt gtatcattga ttatttcttc
     1321 attcaatata ttaagaagga atccaaaaga attattaatt gacaataatt aaaattttcg
     1381 gtgcttttta gcaccaaaaa tgagagtgta atataaaccg tgtaagtaac catcaatcct
     1441 aggcgggtgg ttgcttcacg gtttttattt ttctaaaata agatcaagag gtgattacac
     1501 gatggctaga aagaaaagaa atcctgatgc tgagaagtta gctgaatcca ttttgaatac
     1561 ttatcaaccc gaatctgtag aggatatgca agatgtttta aaagatgtgt ttggtccctt
     1621 atttgaaaaa atgcttcaag gagaattgaa taaccattta gggtatgacg cccattctaa
     1681 agagccaaag aaacatgaca atcgacgaaa cggttatggc aataagacgc ttaaaacgag
     1741 ctttggtaaa gtaaacattg atgttcccag agaccgggag gcttcttttg aacctgagtt
     1801 gattcctaat agaaaacgag atgtctccga catcgcagac tgtagacaaa agctgttcca
     1861 aactcgtaac tcgagcttgg aacagctttt tatctacttc gtttcgaatt tttgaacaaa
     1921 aaaataaagg ctccttgcct ttccatagcc aggtggctaa tttctttaaa ttcattgcag
     1981 caaacactag catcgtatgc gtcgcgactt tttccaagcc tcgatacttg gtccaacgca
     2041 tcccatgctt ttcttttgca tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt
     2101 aaattgattt attcagatct gttaggcgaa gatgttccac ttcatccatc agatctcccc
     2161 aaacatgtct cgtgatgact cgttgatgat ttttacttat ggtacattgg tttagcagag
     2221 gacagttcac acattctttt ggattggatt tgtattctcg gtacccttct cgagtaaccg
     2281 ttgaaaaagc gagcatttga tcttgcggac aaatataaca atcaaaatac tcgtcataga
     2341 gaaactcatt ttttgataat agctcctttt tccctttggg ccttgaataa ggcaaacaag
     2401 gtcgaaggtt caaatgagtc aaaaagtgaa caaacttagg tgtcttatac ccagcatcag
     2461 cgacaacaca attgatttca ggaaaaagtc tcttcgattg tttga
//
LOCUS       DACDHL010000189         2460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDHL010000189 DACDHL010000000
VERSION     DACDHL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.196"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(151..2304)
                     /locus_tag="IV228_002880"
     CDS             complement(151..2304)
                     /locus_tag="IV228_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8718711.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          636 a          550 c          421 g          853 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaacaagaa gtatttaata tttttttcat aaaatctttc
     2401 atcatttcct atccttccgc ctatttacat tcatgcttca ttgtacccaa aaacttctta
//
LOCUS       DACDHL010000190         2439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDHL010000190 DACDHL010000000
VERSION     DACDHL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.197"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IV228_002881"
     CDS             <1..382
                     /locus_tag="IV228_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718712.1"
                     /translation="RITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDNPYSQFLI
                     TVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMT
                     VNQICEITNVSRASLYRKLSEVNN"
     gene            596..1291
                     /gene="vanR"
                     /locus_tag="IV228_002882"
     CDS             596..1291
                     /gene="vanR"
                     /locus_tag="IV228_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8718713.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1269..2423
                     /locus_tag="IV228_002883"
     CDS             1269..2423
                     /locus_tag="IV228_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8718714.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          840 a          437 c          515 g          647 t
ORIGIN      
        1 tcgaatcact cgtagtacac aagatctatt tgaattaatc gataacatac gagataaaaa
       61 ggcaagttta aaatcactaa aagatacatg gcttgattta tcagaagata atccatacag
      121 ccaattctta attactgtaa tggctggtgt taaccaatta gagcgagatc ttattcggat
      181 gagacaacgt gaagggattg aattggctaa gaaagaagga aagtttaaag gtcgattaaa
      241 gaagtatcat aaaaatcacg caggaatgaa ttatgcggta aagctatata aagaaggaaa
      301 tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat tatacaggaa
      361 attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata tttttaaaga
      421 agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc cctttgataa
      481 aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc ttataaattc
      541 ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga taactatgag
      601 cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg aattatactt
      661 aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat tggaatgtat
      721 agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca caagcggcct
      781 tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc tgaccgggaa
      841 agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt atataacgaa
      901 gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc gatacaaaaa
      961 attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc ttgtcattaa
     1021 tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc ccaccgagtt
     1081 ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg agctgctatt
     1141 tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca ccgtgcatat
     1201 ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata taaaaacggt
     1261 atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa ctagaacgaa
     1321 aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg ttgtatattc
     1381 gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa aacaaatatg
     1441 acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac aatatagata
     1501 tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc atgctttcaa
     1561 aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt cagaacgaag
     1621 ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc aacacattaa
     1681 aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga aaaaatgacg
     1741 ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc ggttatttga
     1801 gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat gtgcatatca
     1861 cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag attacacggt
     1921 ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat atgctggtgc
     1981 agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg gttattcacg
     2041 cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc tttaacaaca
     2101 ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt accgcgggcc
     2161 tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca aaagataagc
     2221 tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc gatacgggtg
     2281 gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga gggcagattt
     2341 acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg atgccagact
     2401 tggttgataa aaggaggtcc taagagatgt atataattt
//
LOCUS       DACDHL010000191         2415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDHL010000191 DACDHL010000000
VERSION     DACDHL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.198"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(734..1912)
                     /locus_tag="IV228_002884"
                     /pseudo
     CDS             complement(734..1912)
                     /locus_tag="IV228_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1942..2352
                     /locus_tag="IV228_002885"
     CDS             1942..2352
                     /locus_tag="IV228_002885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718715.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
BASE COUNT          779 a          386 c          440 g          810 t
ORIGIN      
        1 taatgaaaaa cgagaaggaa gatgacttta aagtagttta gtatgaaggt cgattgataa
       61 aacgatgtgg attaatgcta aataatgagt aatttttcaa taatacaaaa actgtgtaca
      121 gtattagaag tagagaaacg ctagcgtcga aaaaaagata tttttcgtga aaagtaatag
      181 gggctaaaaa gaaagtatac aatttattta tgtttctaag catataaaaa agagcgttga
      241 aaggaatatt tccccttcaa cgcttctttt ttagaagatt ttaatgacca tctcaacttc
      301 attttcaggt ttaggtaatt catggaagct tgtgtttatc gtgcttaatt gatcaactaa
      361 ccatgtttta ctttttgact cgtagacaaa tctaaaatta atatcattgt tctcgtgttg
      421 aagaggtttc tccaaataat ctggtgaata ttcatagtca acgtaacctg ctaatacaaa
      481 agaaagcagt ccattattta gtgtcagatt taacggatgg tcgtaagctt ttattttttt
      541 aatgtcatat tgtacatctt atgaagactt aatgactact tgtagtcaat agatttaaag
      601 tatttatttt ctcatttatg tatcaagaat aaatactgat ataataaaaa ttatatcaat
      661 gttttgatga agtaatttgg agggagcgtc aataattttg tgtaaataac tcgtccttct
      721 gcaaaataat gggttactca gtaaacattg aagctaatgt atccgtcact tgttggaagc
      781 ctttatggct tctatttaag aatttttgat tgtatgtatc aaagatggat actaggaaac
      841 gctctagtga ttcttcattt tgaaactgct cttttctgcg gctgtatttc ttgatttgtt
      901 tcttgaaaga ttcgattaaa ttagttgagt aaatgcttcg gcggatacca ggcggaaact
      961 cataaaaagt caataaatct tgatttttta tcagtgactg cgtcacttta ggataagttt
     1021 tttgctactt ctcaatcata ctccctaaaa aggtatttgc ctcttccttt gagctagctt
     1081 gataaacagc cttaaaatca tcacagattt cttttcgatc tttgacacgt actttatgag
     1141 caatattacg ggaaacgtga acacagcaat gttggtattt agctttagga taaatctggt
     1201 gaatcgtatc tttcatgcct tttaagccat cggtaataaa cagtaagaca tcatgaaccc
     1261 ctctggagta aatgtcctgt aacaactcat tccaaacgta ggtcgattca gtcggagcaa
     1321 tcgcataact tagtacttct ttagtgccgt cttctcgtat accaatggca atatagattg
     1381 cttctttgga gacggtttgc cgttttaaag gaatataagt agcatccatg aaaatagcga
     1441 catacttatc atttagagtt ctaactttaa atgcatttac ttcttcagtc agcactttag
     1501 tcatgttgga catggtttgg ggcgtgtagt gatgcccata cattttctca atcaagtctg
     1561 cgatttccga catggtaata ccttttcgga agaggtgaat gacggtctcc tctaagatat
     1621 cattcgtccg tctataagca ggaacagtct gttgcttgaa ctcgccgttg cggtcgcgcg
     1681 gaatctggag atgaagttcc ccgtactcgg tcttgaccgt acggtcatag gagccgttac
     1741 gagaattccc cgtgttaaaa ccaatgcgat cgtacttttc gtaatcgagg aaagccgtta
     1801 actccgtttt gagaagcgta ttgacagctt tttccaaatg gacacggaat aattcattca
     1861 aatcgccttt gttcgctaga gtctttagaa tttctgtagt aaaatcgttc atatggaagt
     1921 cctcttttct gtgaatgtgt tgtggtaact ctattctaca gaagggactt ccttttttct
     1981 atttacacaa aatattttac actctcgtca aatataaaaa ctagtacgaa attaatttgt
     2041 aaattattta agcttgataa aataagcaat atcgatgatg taaagtccaa gatattaaca
     2101 agaaaagtaa gcgaaccagt aaagctaacg gcacaagata aaaaaaatgg aattgttctt
     2161 tatggtgatg atgcaaagta taactataat tgggatacct cctcagaagc tgaggtatta
     2221 agtattccta ctaccgatac ttcaggggaa aaagtcacct ttaaaataaa ttttataacg
     2281 gcaatttcta agttggagta tgggaagcaa aaaaagagag ataacaatga tcaatatcta
     2341 cctaaaaaat aacgaattaa tgatgcatat gttaaagttg tttttttgaa ttaaacaact
     2401 caatttattc agtta
//
LOCUS       DACDHL010000192         2411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDHL010000192 DACDHL010000000
VERSION     DACDHL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.199"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..144
                     /locus_tag="IV228_002886"
     CDS             <1..144
                     /locus_tag="IV228_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8718716.1"
                     /translation="PFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAK
                     DHY"
     gene            complement(329..931)
                     /locus_tag="IV228_002887"
     CDS             complement(329..931)
                     /locus_tag="IV228_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016612517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718717.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITQRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAISKIAKDYNVTRQTVYRIKNELM"
     gene            1245..1667
                     /locus_tag="IV228_002888"
     CDS             1245..1667
                     /locus_tag="IV228_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718718.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1677..1880
                     /locus_tag="IV228_002889"
     CDS             1677..1880
                     /locus_tag="IV228_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718719.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2082..>2411
                     /locus_tag="IV228_002890"
     CDS             2082..>2411
                     /locus_tag="IV228_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718720.1"
                     /translation="MIFLKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSN
                     RPGLEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKS
                     SSTGQLMF"
BASE COUNT          825 a          368 c          405 g          813 t
ORIGIN      
        1 ccctttgctg gattaggaaa acctgagcca ttaaagcatg atttatctgg aaagtggtcc
       61 agaagaatta cagatgaaca tagactgata tatagagttg aaaatgaaac gatatttatt
      121 tattctgcaa aagatcacta ttaaccaatt ggaagtaagg gaagggtcag aaacttttaa
      181 gtttttgacc tctatttttt tcattacatt acgtttctta gcaattcatt taataaatgg
      241 ataaaagacg acatgcctgt ataaagttaa tattggagaa tttgtaatat tcttacaccg
      301 aaaatatatt tattcagaca agtaaatgtc acatcagctc attttttatt ctataaactg
      361 tttgtcttgt aacattgtag tctttagcaa tctttgaaat agcaacacct tttttaagat
      421 cttcaacgat gtttttataa acaaggcgcc gttggggatc ttttgcatta gcactgtata
      481 gcttaggacg tcctttatag actccatttg ctttcgcaat cttaattcct tgcgcctggc
      541 gtcgtttaga ttcggtacgc tcttgttccg cgatcatggc caaaatttga atgatcaaat
      601 ctttaatgaa tttatccaga agaggattgc caatggcttc agccatgata ggtaaactgg
      661 tgatgatcag ctgcacattt ttcttcttta ggtaattgac tgaagtgatg atctcgtcgt
      721 aatttcgtcc aagtcgatca atagcttcca caataaaaat atcttgatct cggacgaaat
      781 ccaatgcctc ttgaaaaaca ggtcgctgag taatcgttgc gccagattgt ttctcgatga
      841 aaattctctc tgcaccgaat tttttcattt cttcaatctg ccgttgttca ttttgatcga
      901 tggatgagac acgtatgtat gcaactctca aaatttttac ctactttctg aaactattta
      961 aaatacaccc taatcataca caaaaaaaac agatttttca atgtcgtggt cttgtctatt
     1021 tagggtatac cttatataga caaaaactaa tgatggagga aatagtatgt gtcacttttc
     1081 tttttaaatg gcaatatttt ttgtaaataa atgtcaatca agaaaattta aacgattatg
     1141 acattaaaaa tatattttat gaattttcta gtatataact tgatttatct gttaagattt
     1201 attaagaaaa cgttttctta tttttgattt cgaggtgata gacaatgaaa aataagttta
     1261 ttttatcttt aggtatttta actattggaa ttagtgtaac tggaactaca attcctacat
     1321 ttgctgatag tacaaatgaa ccaactactg tagtagataa taaccaagaa ttaactcctt
     1381 tagataggaa cggaatagcc attggtaacc caactaacga cttaactgtt actgaaaatt
     1441 atttaatagt ttctccatta cttgctcgag cagcttctaa aacgatacga gtgacaggaa
     1501 cgatgatttt tgataaagtt ggttggcctt ctagacctcc tcaatcacgg aattggaaag
     1561 aaaaaagaaa tggttattgg tactatggaa aattgtattt acaaagttat aaatcagctg
     1621 gaaacaattg gattgcgact tattcaggaa ccttaactag aaagtaggga ttttttatgc
     1681 cagaatttgt gaacacacca aatttttatc aagatagcga tattctaagt aaaattaatt
     1741 taccgaaaga agaatcagct ttacaagttc agcgaacttt acaggagaca caaaaaattc
     1801 aaaaatcaat ttctaatcta gaagattctg aagaaaaaag aaaaaaaaga gaccaaaaaa
     1861 catatcaaaa agaaaaataa tgattttaaa aaaagagtgt ctagtcgaca ttcttttttt
     1921 ctaattgaaa gtgttcataa aatcacccat ttctggacac tttctttttt tgctttattt
     1981 ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg ttcagttaaa
     2041 ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt ttgaaaatag
     2101 gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac agcctaaata
     2161 aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt aatcgaccag
     2221 gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt tggcgcttag
     2281 atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg aatgagcgtg
     2341 gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt tctacaggac
     2401 agctcatgtt t
//
LOCUS       DACDHL010000193         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDHL010000193 DACDHL010000000
VERSION     DACDHL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.200"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IV228_002891"
     CDS             207..2069
                     /locus_tag="IV228_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8718721.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IV228_002892"
     CDS             2069..>2400
                     /locus_tag="IV228_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8718722.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDHL010000194         2391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDHL010000194 DACDHL010000000
VERSION     DACDHL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.201"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(407..724)
                     /locus_tag="IV228_002893"
     CDS             complement(407..724)
                     /locus_tag="IV228_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8718723.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(725..979)
                     /locus_tag="IV228_002894"
     CDS             complement(725..979)
                     /locus_tag="IV228_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8718724.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1239..1457)
                     /locus_tag="IV228_002895"
                     /pseudo
     CDS             complement(1239..1457)
                     /locus_tag="IV228_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          780 a          435 c          344 g          832 t
ORIGIN      
        1 agtttcccat agatatattg gacttcctct tttaaaacac aaaaataaat actaccttat
       61 ttaggtagta tttattaaaa gttacgtcaa ttttgattaa aaatatacta cttgaacctc
      121 tcatgagtaa agtcacgaaa tttttgggaa tagagcgtac ttgatatctt attttttcac
      181 taatagcagt gtctcttatt caccaagcga tccccgtagt tcctacggtt cttaattttt
      241 ctctagacta atgattgtat ctttagacct tcggccataa catggagcaa aataaaaagg
      301 tactaagtct tatatatcaa tcttcgagta accgacggtt aacaaccata tcgagcggca
      361 ttaatcaccc acctgtagag gatgagcgac ttttgcctaa ttattgttaa aagcctagtt
      421 tcatttgttt agattggaaa atacgtccta caagtatctc ttttttttca tggtcgatcc
      481 gataaaataa ggcatagctt ttgccgatca cgatccgata tgttgggata tccattccat
      541 agatttcgga aacttcctca tgcatagttg gaaaattttt ggttagttct aaagccttct
      601 ggatcgcacc tacaaatctt tttatgctgg ctggagaaag agcaagctca ttttcccatt
      661 cttctattat cgtttcaagg ctggtctgga aacttttaga ataaactagc gtgtactctt
      721 tcatctattc actccagccg tattttccga aagcttttac catttcaatt tctgaaactg
      781 ttaaaccttt ctcaaagtct tggaaacttt cttcaatcaa tttgcgatcg tgttcttttg
      841 ctatttgagt agtatctaat atgaataagt tgccttcttg tttggcttgg atagtgtctc
      901 cgctttttaa tccaagagag cttggaagga tcactcccac gctattgccg atctttctaa
      961 cctttagttc ttttcccatg ttatcacact tcttttagtt tttgataaat aaattataac
     1021 atgttataac gacaattgcc aacggtgttt cttaagggat tccactccat attattattg
     1081 gacaaaataa gccacttaga acgctgaaaa catatacaaa cgatatcaaa atacgagtga
     1141 actactcacc atacacttcc gttaacatcc atttaagtag gtatttttat tatatataga
     1201 tttaacatat gtcggtcggt tgattgatat agctaaagtt ataactcaac cgaagccgat
     1261 tcatctacac gaataaagtt gcgagtgttc tcggctaatt cataaaaatt gttatgtgtt
     1321 ccatatagca atactggaat aactttattt tcttgaatct cataagcaac tctatacacg
     1381 gtgaccgcat acttaaatcc attagaccaa ttacctgata aacctccagt tttttgctct
     1441 tacttaaatg gatcaatagc aatttcatca caaacctata gaggatgagt gacttctgcc
     1501 taattacgtt aaaaaatatt gaaagtaact ctatgtagca aaaaatatct agaatttcat
     1561 ttcctcaaaa ctgatcgata ttgtatctaa aaatatatac tacctaaaat aggtagtata
     1621 tattaaaata tgtgcttttg ttagaaaaat caatacataa attatcatat ctgtatatgt
     1681 catttaggta tttcagttat gttataatag ttttgctgtc tgctaccccg gcctaatctg
     1741 gcaaggggag gaggagatat aatgaaatac ctttttgatt ttgtttttgc aatcaagcca
     1801 tttgcagagg taactgcaca ctacatctgc aagtggcttg attgcaaaat tgccaaaaaa
     1861 gacagtaagc ctaccagtta attctataat atagaaacgt aaaaaaaacc ccatcagcct
     1921 acacgctgat ggggtttgat gatataatga aatacctctt tgttactcat aattatacca
     1981 taattagtca cgaatgcaac caatagaaaa aactacttag aacggtagat catcgctaat
     2041 aacaatgcat tgtccaccaa atggattttg tgaagcatta aatacattgt tattaattgt
     2101 cgctgttttt aactaggcct ctccccttct attataaaag aagatatata ttatttctta
     2161 attacctact accttaaacc aaaatttaag tactacctaa tttaagtagt acttaaataa
     2221 tctaaaaaat agttatccca attgttatgc ttttataata cgattatttg taacaaaatg
     2281 aatattatat aattaaacac taccatattt aggtagtgtt taattattag caaaacaaaa
     2341 aatatttatt ctattctctt aacttcaact aagttatgct gcacaccttt a
//
LOCUS       DACDHL010000195         2311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDHL010000195 DACDHL010000000
VERSION     DACDHL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.202"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1307)
                     /locus_tag="IV228_002896"
     CDS             complement(<1..1307)
                     /locus_tag="IV228_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8718725.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIR"
     gene            complement(1326..1730)
                     /locus_tag="IV228_002897"
     CDS             complement(1326..1730)
                     /locus_tag="IV228_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8718726.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(1962..2219)
                     /locus_tag="IV228_002898"
     CDS             complement(1962..2219)
                     /locus_tag="IV228_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8718727.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          702 a          448 c          322 g          839 t
ORIGIN      
        1 cgaatctctt gataggtggg atatgttaaa gctgaattat tttggatatc aacattcttt
       61 tgtctaaaat atctatcata tttctcaaaa agctgctgct tatcagtgtc tttcttccac
      121 gtttgataaa gatactctat aagttcgttg atattaaaaa cttcgtctac tttttccaaa
      181 tcatccattt gaatatattt taattcattc tgtcttttca taaccttcat gccgttgaaa
      241 ttcacccatt gtggtaaatg atacatctga atataaagtt cttccaaatc atctaagaaa
      301 taaaaggcat tggcacaggt caattgaatg atatttttca aattgccaac attttcacta
      361 aagcgatagt ttaataaaaa atctaataat tgttgagata tcagtatttt ctttgctagt
      421 ctttcttctt ctgatttaaa aaatatcttg attaactgtt ttctttcata aagagaacga
      481 ctgtctaatg ttggaagtgt tagctttaca ggtattcttc tcaacaaagt tgataatagt
      541 ttatcctcag gtggttctga tgttgcaaat ataatccagc aacttgatgt gtaccagttt
      601 tcattatcac ccatccgatg aaatttcctc gtatccataa actgaaaaag tttctcctga
      661 cattcattgc tcaatgcatg aatttcatct aaaaataaga ctccattatt agctaattct
      721 gctaaaccct ttcttccatc ttcggctcct gtatatgcac cttttacatg cccaaaaaaa
      781 ttggacaaga aaagttctgg attattggca tattcactac aattaactga aatgaaagat
      841 gccttctctt ctatataatt attggccaac aaatccttat acacataatt tgctaaatag
      901 ctttttccag ttccagtctc gccatgaagt aaaatgggta agccatttgg cggatagtag
      961 attgctgctt taatttgagc aattacattt cgcagacttc cttgacttcc gataaccctt
     1021 tctattccaa ccctaaaaga gctatcatac catttttccg cttcctctga agagctaaaa
     1081 atttgttgat ttggaacttt ctccggtaaa gaaaagaaga ttactggacg agtattgatt
     1141 tttccaaatt ctccatcttt tacgaattca ttcaaatatt gagagacaat attacgacta
     1201 acatttaatt tttcagatat tttctgtgct gtattgttcc cccatttatt tttaatctca
     1261 gagttctcta tgacataatt gacaatgcgt tcttttaatt cttccatcac agcacctcct
     1321 cgacactatt cgaattttgc cgacagtatt tttccataaa tatgacttgc taaaaaggct
     1381 ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa gacgttgaaa
     1441 agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat cagatcttct
     1501 tttgattctt catctgaaag taattcacga tacaacatcg tttgtttgct tactgtgtca
     1561 tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg gtcggataaa
     1621 tatttataga aaatcagccc caataaataa tttttatatt ctgacgcatc cattttacta
     1681 cggagattat ctgccgcact aaataacttc tgatttaatt ctgcacccaa aaagatacac
     1741 tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc agttttagaa
     1801 aaagcaaaaa aataaagaaa cttattcttg ataacaagct acgtgacgta tatccctaac
     1861 acaaaaatag ccatatcctt tggtttggat acggctattt gatattactc attcaattat
     1921 caaatcacag taggtgtgaa tctcatcggg atcggcaata tctacttctg attaggatca
     1981 aaggtgattt tctcaataaa ggtgtgatac aaagctttaa cttgcttctt ctcactctgg
     2041 ataagaaagc aatccaaact agtcaatatc tgttggacat ctttgacttg aatcggttcc
     2101 ccttgttgtt gaaagatccc taagatttca ttctctcgaa tttgtaactg tcttcgtttt
     2161 tcagtcaatt catttaaacg atcttttaac atcggaaaca attcgggact atcttccaaa
     2221 gcagcttcct gtttttgctc taatagttag attttgcgta ccattttctt cgtttatttt
     2281 ccttcgattg ctgattttaa tatagtctaa a
//
LOCUS       DACDHL010000196         2227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDHL010000196 DACDHL010000000
VERSION     DACDHL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.203"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..905)
                     /locus_tag="IV228_002899"
                     /pseudo
     CDS             complement(1..905)
                     /locus_tag="IV228_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1180..1722
                     /locus_tag="IV228_002900"
     CDS             1180..1722
                     /locus_tag="IV228_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718728.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1812..>2227
                     /locus_tag="IV228_002901"
     CDS             1812..>2227
                     /locus_tag="IV228_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8718729.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          762 a          346 c          421 g          698 t
ORIGIN      
        1 actcaatgaa cgagtaattc caaaaccatt cagaatctca tgaatggttt ggctatcaaa
       61 ttcctttcca cggtctgtat gaaaaagctt gacgtctgtt aaagaatacg gtatccgtcc
      121 aaaggcttct tttaccaatg aggcatcttt cttctcacca caggaataac caataatttc
      181 tcgattaaac aaatcaagta ttaagcagat ataatgccac tttttcctca cgcgaacata
      241 agtaagatcc gtaacgatgg cttccaatgg ctgttcttgt gtaaaggtcc gatctaatac
      301 attcgttgtt ttcgcttcat tacaagctgt tcgttgccct ttaaaatgag cgatcgtata
      361 ggtagatgtc aatccgcggc gtttcatgat tcgaccgatt cggcgccgac tgagttgaag
      421 cccacgcttt gctaaacact tcttcaattt tcgggtaccg taagcctttc ggtttcgaat
      481 aaactcttcc tgaacgatct cttctaagtc ggactcattt tcgatcggtt tcgcttgata
      541 atagtaggtc tgacgagaaa tatttagaat tttgcacatc gctgatatgg aatatttatg
      601 cttgttggca tcaattactt gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata
      661 tcattctcca tttcgagttg cttatttttc tttcttagtg cgatcaattc tgcttgttct
      721 ggtgttaagt tgtctctttc tttgaatgag cccgttgatt gtgcttgctt catccattta
      781 tcgaaagaag aaggcgtaag ctcatactct cgaataattt ctgcgcgagg cttaccagcg
      841 agatagagat cgacgatttg ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt
      901 gacatgaaaa atcctccagt gtgtttttta ttattctaca caccttattt tttctgtcta
      961 gtctagtgta gccgattcaa attgatgaag cagatcccaa agggctgctt catcaatttt
     1021 ttcgtaagtg tccgtacact tactgtgctt gctcatatat taatcgatca tatactaatc
     1081 gtgtaagaac aactttaagc ataatactta tattgttata ccatagtttg gaaaaataaa
     1141 aaattatatt tcatcaacgt ttcataaaag gtgtgtagaa tgttattgaa agtagctata
     1201 aaatgctatt gtgtaaaaaa taaaggagga ataatttatg aaaaaatagt accaagagga
     1261 gcatgcctat tacttgcatt atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat
     1321 gaaaacaata aaataaaaga aggacaatcc ataagttctg aagaggaagg catgtcaatt
     1381 aatgcaaatg gcaaaaatga aacttttaag ccatcagatt atacattgga ggcaaagaaa
     1441 gaatatgttt atgaatattt agggcttaaa tttaaactct cagacaaatt tagaaattat
     1501 atagctgata aaaagatagc gatgttagat gatcaaagtc ctatagataa agaactaaag
     1561 tatgctattt tgacatttga gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag
     1621 atgggcgatg gatataaaaa ttggcaaaat gagcttgaaa gaattgggac tattggtata
     1681 tttgaaaaaa atacatcaga agaaaaaaat ctaaaattat aaaatgtgat actcatacta
     1741 aaataggagt ttcaagtgat ggaaaatacg attgctattt aagcacaaat agcggagctg
     1801 aaagtaatct tctggatgaa ttaaaaagaa ctgagattca aattatagat aaaaaagaac
     1861 gtcctaaaaa tggttttgtg ctatcagaaa aaactgattt agaaaataca gaagcattta
     1921 acaaagaatc ggttaaagat ttacgtaaac taccaacaaa ggacataaat ggcaaggatt
     1981 ttacaagtaa agattttgag aagtatgatc ttactatggt aaatgtattt gcaacttggt
     2041 gtacagcttg tgtaaaggaa attcctgatt tggttgaggt tcagaatgaa atgaaaagca
     2101 agggtgtaaa tatcgttgga gttgttacag atcctgttga tgataacggt gaaaacaagg
     2161 aagcaattga aaagtcaaag ttaatacatg aaaagacaaa ggcttcatat cctttcttaa
     2221 tgccaga
//
LOCUS       DACDHL010000197         2201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDHL010000197 DACDHL010000000
VERSION     DACDHL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.204"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            194..>2201
                     /locus_tag="IV228_002902"
     CDS             194..>2201
                     /locus_tag="IV228_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8718730.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT          738 a          399 c          376 g          688 t
ORIGIN      
        1 aatgctatct gataaattag gtataaaagg aagaggaaat tttcttgctg tatttttgat
       61 tgtcactcaa gttatatgta atttcccttt agtttcttat cgcgtatcta catttatgaa
      121 acgtgaattt ccaaactcag ggaaggcatg gctgataaat atcgggcagt tttttatttg
      181 ctatttatta gcagtgtccc tcctagttat ctttttattt tatatacaaa aaatccgttt
      241 cattcatcgt ttcatcgaat acttcgcttt tgattcgatt gaacaattaa ctcagcgttg
      301 gaaaaaaaga attcattggc tttttgttca taaaagttat tttttagttg cagcattttt
      361 ttactgcttt accttcgtgc agattttcgt tttagaacca aaagaaggtt ggaaagaaac
      421 tatccaggtt gccttaaaaa tatttactca gcgacaagca cccatgatcc tgacaatcat
      481 cattattatg ggatttttct tcttactatt gttgatttcc aatcgttttt ggtacgcctt
      541 gactgctacc ttgatcatta acttactgct cactatttcg acagtaatca aaatggaaat
      601 gcgggaagag cctgtctttc cttcagattt aaaaatactg accggattgt cagaactatt
      661 atcgatggtt agcccagtct tattaatcgt gggcggattg atccttctcc tattattgat
      721 tactagtatt attgtacaac gtagactaca aaaacagtat tccttgaaga ttcattggaa
      781 acgtcgattt atcagtatcg ctgtattatt aggatgtttt tcgggtgtct ttttcattaa
      841 tcacaaaaat tcgccttctt tcttgctgtt taatttattt aaagtaaaca agactttctt
      901 taaccagcaa ggcgccgtaa aagataacgg accagtcatt caattcctaa acaatatcga
      961 cattaaaatc atggaagaac ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa
     1021 aaagtacaac acaaaggcag atgaaatcaa ccaaacaaga agtgactggt taaacaatca
     1081 gacgatcgtt ctcaatttaa gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac
     1141 agtacctaca gaccctattc cttacatcaa aagcattata acagaaggaa caggaggatt
     1201 gatgttatcc gtcggttatg gtggcggaac agctaacatg gaatgggaag gactgacagg
     1261 cttgagtatc agtaacctgt ctgcttcgtt agttacacct tatacccagc tagtggagcg
     1321 tcagaaaata agtccaaata ttaccgatct attcgatgaa aaaatagcca tccatccttt
     1381 tacagctgct ctttatcgca gaaaacaagt gtttgataaa tttggttttg acaggttcta
     1441 ttatttagac agtccggata aattaactta tacggataaa atagaacaaa gcccacgcat
     1501 atcggatgat tcagcataca aagaaacctt gaaagcttta caaagcaata cagaaacgtc
     1561 acaattcatc caattatcca ctatgcaaaa ccatatgcct tatgatgatt actatagtga
     1621 attgaactac actgctgaag gaactgctgt cattcctaat agaagaaaag aattacaaac
     1681 ttatatgcaa ggccttcact ataccgatac agcagtaaaa aaattcatcc aagaaatcga
     1741 caaaatcgat aaaccgatta cctttgtttt ttatggtgac catttacccg caatctatag
     1801 tgggaatgat tcgaaaaaat atggattaga acagcaccag acagattatt tcatttataa
     1861 caacaagtat agtcgtgaac aagcagctac gttaaataaa gaagttgttg ctcctaataa
     1921 ctttggtgct atggctcttg agcatgcgaa tatcaaagta actccttact atgcgttgtt
     1981 gacagaagta tctaatcatc tacctgcatt aacaattgat cctacagaaa gcttgtctaa
     2041 tcgttttaat ggaaaacagg tctttgttac agaggatagt caaattatcc aagaaaaaaa
     2101 tttaactgag aaacaaaaag aaattctaga agattatcgt ctgatacaat atgatcttgt
     2161 tgcaggtaac cagtacagtg caacatgggc gacacaaaaa a
//
LOCUS       DACDHL010000198         2199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDHL010000198 DACDHL010000000
VERSION     DACDHL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.205"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..717
                     /locus_tag="IV228_002903"
     CDS             1..717
                     /locus_tag="IV228_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8718731.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            916..>2199
                     /locus_tag="IV228_002904"
     CDS             916..>2199
                     /locus_tag="IV228_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8718732.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTF"
BASE COUNT          721 a          428 c          426 g          624 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tcttttattt tttggctcta tactttttga tgttggtgaa cttcaccaac atcttttttg
      841 tataaaaaaa tatagaaaag acaccctgaa atcctttaga attaatttgg ctaccaattc
      901 ataggaggat tcaagatgtc tcatactcag cttatcaaag ataccctaaa tattctagac
      961 ctaaatatct atttcgagga aaattgttta actaaagaaa aatacaaagg gcaaatttgc
     1021 ttgatttaca aaggaactct tctgtacaag ccagaagagt gttcccattg tctttgtgtc
     1081 gtcccctcta ggatcattcg ttgggggacg acaaccgttc ggttgttact gaatgatgtg
     1141 tctgagtatc gcacttatct ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa
     1201 agaacattcg tagcggatac ctctgttgct gaaaaacact gtttcattag tcaaaaagtt
     1261 cgttggtctg tcattgctcg cttaaaagag aatacttcca tgacggaaat cgctcgtcag
     1321 aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc gattctaccg cccattgaat
     1381 cctttcaaac agactttgcc aaaggtctta tgctttgatg aattcaaatc ggttcgaagt
     1441 gttgccagtg caatgagttt tatcatgatg gacggacaaa gccatgcatt attggatatt
     1501 gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt ctcggttttc attagcgact
     1561 cgtgaagccg ttcaattgat tgttattgat atgtatgcgc cttatgtttc gttggtgaaa
     1621 aagcttttcc cgaatgctca gttgatcatc gatcgtttcc acatcgtcca acatattggc
     1681 agaactttct tgaatcatcg agtgaaagaa accacggtac gacttaaagg aaattcccac
     1741 tctcaacgag ggttagggaa aaaattgaag cgctattgga aactcctaca aaaagaagaa
     1801 gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt ttaaagatta tttgactgaa
     1861 agtgagattg ccgatcgact gttagctgct tgtcccaact tacgccaagg ctatcagtta
     1921 taccaagaca ttctttatgc ggtaaagaaa agagatcagt cactatttga ggattgttta
     1981 actagagaca ttagcggtct gccagaaacc tatacaacca ctttacgaac gtttaaaaag
     2041 tttctgccac agatccaaaa tgcgcttcac tacagctatt caaacggacc attggaatgc
     2101 ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg gctttcgcaa cttttacaat
     2161 ttcaaactac gaattatgat tcgtcatggc aaaacattt
//
LOCUS       DACDHL010000199         2164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDHL010000199 DACDHL010000000
VERSION     DACDHL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.206"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            486..>2164
                     /gene="ltrA"
                     /locus_tag="IV228_002905"
     CDS             486..>2164
                     /gene="ltrA"
                     /locus_tag="IV228_002905"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8718733.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHC"
BASE COUNT          838 a          336 c          469 g          521 t
ORIGIN      
        1 ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg tacaaacgta ctgacgaact
       61 agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg gagagacagt gagcaaaaaa
      121 tcactcattg tgctaacttg tggttgagta aaagtcgttg ttatgaaaga ccatgctgcg
      181 ttacaggcgt aggagaagtt agggggcaaa agctagcatc taagttaagg catgtggata
      241 gatataaggg aacaggggaa gccatcaaca tggagagtgc atactcgatg aagtggtagt
      301 aagaaatatg tgaatgacaa ataacttact ttagtgatgg tgaaagtaga gtcacgactg
      361 atgaaggttc tgtaatggaa ctggaggaac aggctcgatt cgtctgtaaa agtcatgaaa
      421 cccgttcaac catgggattc tcgtatgact aaaaaggtca cgagccaatt ggagcgtgat
      481 aaaccatgac aaaagaaaca agtccaaaag ataggaagtt gcggcatgca gaatattatg
      541 gcatgacgga aatcttcgat aagatatact ccaaaagttt gaagggagat aatttcaaga
      601 atctaatgca gattatcatt tctgataaca acattgtcct agcatatcgt aatatcaaga
      661 gaaatcacgg tagtgtgaca agaggagaag atggagtaac gattaaagat attgaaaatc
      721 tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt cgcacattat aaccctcgga
      781 aagtaaggcg tgtagaaatt cctaaatata atggtaaatt aagaccgttg ggtattccat
      841 caatatggga tagaatcgcg cagcaatgca tcttacaagt attagaacca atatgtgaag
      901 caaaattcaa taatcacagc tatggtttcc gcccaaatag atcagcagaa cacgcaatag
      961 cagattgtat ggcccgaatg aatcaaagtc acatggaata tgtggtagat gtagatatca
     1021 aaggtttctt cgacgaagtc aatcatacta aactcatgag acaaatctgg tctatgggta
     1081 tacgagacaa acagcttctc ttaatcatta ggaaaatgtt aagagcaccc gtagtacttc
     1141 caaatgggaa gttgatatac ccaacaaaag gaacaccaca aggcggaata cttagcccat
     1201 tacttgcgaa catcaatctc aacgaatttg actggtggat agcaaatcag tgggaggaaa
     1261 aagatgccaa agagctaaaa catcatttta ggaaaaatgg agcttgggat aaaggtaata
     1321 tctatcacaa gctcagaaag agtacaactc taaaagaaat atacctagtg agatacgcag
     1381 atgattttaa aattttctgt agaaattata atgaagcgaa aaaagttttc tatgcaacta
     1441 aaatgtggtt acaggagcgt ctaaagttac caatatctga agaaaagtca aaaatcacaa
     1501 atctttcaaa agaagaaagt gaatttttag gctttacgat gaaattagtg accaaaggta
     1561 acaaaaaggt tgttaatacg catataggta caaaagcaca aagcatgatt aaagtgaaac
     1621 taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag caaagatacc tttatctata
     1681 ttggagtcta taacagtatg gttattggga ttcataatta cttcaaaatt gcgactcatt
     1741 gtagtgtagc aatgcgaaaa attgcaagag aactcagacc aagttttgaa atacgactac
     1801 gagaacaagg aatctcaaaa gaaggacaat acggaaagta cgaaggatac aagccttacc
     1861 tacggtcagg acaaatacga tatataagag ggtttccaat attaccagta gggtatattc
     1921 aacacagaca tccgatgtgt aaaaaaagaa atattaataa atacaccgaa gaaggtaggg
     1981 aagccattca taagaagcaa caaaccgtag aagaatggaa gatatcgtgg ttgtgtagca
     2041 atccagtgct tagtaagaga gcaacgattg aatttgcgga caatcgaatt tcaaaattca
     2101 tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct tatcttatct gaaatgcatt
     2161 gtca
//
LOCUS       DACDHL010000200         2098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDHL010000200 DACDHL010000000
VERSION     DACDHL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.207"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..908
                     /locus_tag="IV228_002906"
     CDS             <1..908
                     /locus_tag="IV228_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP8718734.1"
                     /translation="KDYKGTPRTVLVQRHDELYLVNRTSGENYGLGANIAIVGVGSVG
                     SKIAENLGPLGVRKMTLIDNDCFQAENLYRNSLGADSLKNARSQNNFFKVDLMQEELE
                     KKIPYLCVEVESMNVLDLISMQPGFFKEYDYVFVCIGDTMASLALNNYFSKSSQKVFY
                     SWLEPYGIGGHTLFINYSLNQKGCFNCLYTDSNSKNRCSNRASLVKEGQIFERTLASC
                     RSKFIPYNAIASTECAINAIKLFHKVNQNNMTNSTLFSWIGDGSTFENLGYQYSDRNT
                     FLNGVNKFEERFFSKYCKVCGELDD"
     gene            901..1362
                     /locus_tag="IV228_002907"
     CDS             901..1362
                     /locus_tag="IV228_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718735.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            1375..>2098
                     /locus_tag="IV228_002908"
     CDS             1375..>2098
                     /locus_tag="IV228_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718736.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTS"
BASE COUNT          736 a          265 c          412 g          685 t
ORIGIN      
        1 taaaagatta taagggtacg cctaggactg tattagttca gcgtcatgat gaattatatt
       61 tggtgaatag aacgagcgga gaaaactatg gattgggtgc taatatagct attgttgggg
      121 tagggtcagt tggcagtaaa attgctgaaa atcttggtcc attaggtgtg agaaagatga
      181 cactcataga caatgattgt tttcaagctg aaaatttgta tcgaaattca cttggtgcag
      241 atagtttaaa aaatgcccga tctcagaata atttttttaa agttgattta atgcaggagg
      301 agctcgaaaa aaaaatccca tacctttgtg ttgaagtgga gtcaatgaac gttttagact
      361 taattagtat gcaacctggt tttttcaagg aatatgacta tgtttttgtt tgcataggag
      421 atacaatggc aagcctggct ttaaataatt atttttccaa aagtagtcaa aaagttttct
      481 attcttggct ggagccatat ggcattgggg gacacactct atttataaat tattctctaa
      541 atcaaaaagg ttgtttcaat tgtctttata cagattcaaa ctctaaaaat aggtgttcaa
      601 atagagcttc tctggtaaaa gaaggacaaa tttttgaaag gactttagcg agttgccgct
      661 caaaatttat accatataat gctattgcct cgacggaatg tgcaattaat gcaataaaac
      721 ttttccataa agtcaatcaa aataatatga ccaatagtac tcttttttca tggataggag
      781 atgggagtac ttttgagaat ttaggatatc aatatagcga cagaaatact tttcttaatg
      841 gagtcaataa gtttgaagaa agattctttt ctaaatattg caaagtatgt ggggagttag
      901 atgattaaat tttcgtgtga ccgtattgtc attgaagtaa gggatgaagt tcttaagata
      961 tttgaacaat atcggcaggg aaaggatgac ttgcctgaag ctggaggagt tcttcatgga
     1021 aaaagactta agaatgggag cataataatt accgatataa caacacctca aataggagat
     1081 atacaaaaaa gattactctt taaaaaaaac cgagaaattc atcaacagct ttctaatgag
     1141 aaatggatag aatcaaataa gttttctatt gcattgggag aatggcatac tcatcctgaa
     1201 gctgtaccaa ctccatcaaa tgttgataag aaaagctgga gactgaacgt gagcaagcaa
     1261 catgatgata gagtttatgt ttttattatc gttggatttt ctgatttgag aatgtggtta
     1321 ctgtcaaaaa aatcagatat taggcaggct ataaaactat aaaggagtga ttaggtgaaa
     1381 tcacaaaaga atatttggtg gtgggggttt ttagtaggaa aggtaatgat accagccctt
     1441 ctcttaattt ctctaccgat gattactttt ttctttacca ataaaaaaat gagtaaagaa
     1501 gccgtgattt ttttctttgg agaccaaaaa gctaccttta ttgaaacgtt agtaacatct
     1561 cttcagctta atttgaatta cattttttca attattattg ttatatcgtt attgaatttt
     1621 tttaaaaaaa gaaattcagg aaaggtgttt aactctaatg gaaatgtgta ctacaactat
     1681 ttttattttg tattttgggt agcagcaact ttacttggtt atgataaaat tcagattgca
     1741 ggaattccta tacacatgca atataagttg gttttgagtg gaattttttc tgaggtgttg
     1801 ccagatatat acgatgatca ttacgacagt gatggaacat gtaaagtaag cattgaaaaa
     1861 gaaaatttcg atgacatcga tggatatgac tctgtgaact tactgattat tgatacttac
     1921 gatataaaaa tgagtgaatt atctatggaa aatcaaacat atcctacgat aattgttaga
     1981 gggaattcaa tagatggagt tcgtaaagtt aatagatctt taatcttgga aataaaaaaa
     2041 acaatggatg aaattcaaaa aagtgacttt aaaaaagtat ttgttgcttc aacatcta
//
LOCUS       DACDHL010000201         2011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDHL010000201 DACDHL010000000
VERSION     DACDHL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.208"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(288..1580)
                     /locus_tag="IV228_002909"
     CDS             complement(288..1580)
                     /locus_tag="IV228_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8718737.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          656 a          385 c          325 g          645 t
ORIGIN      
        1 gtaaattcaa ctaaatatgc ctaaaaaaat aaaaaatcta agatagaata agatttattc
       61 agtcttagat ggatttatag caactttttc gtaacaactt taaaaaatac gaaaagaaag
      121 agctgtttta ctctgactac gttgctaaat tgtcgtttaa tcattcattt aaaccaaatc
      181 tttttttata gaattcgttt taatcctcct gaatccaaaa actatgaaaa ccgtaattaa
      241 atagttaaga gtttttaacc accataattc tctcggctat tttgaaatca gataaatttt
      301 gatcggacac atagttgaat aaacctaccg ttgttacgga aggtaaatcg catacttttc
      361 attctagagg agaaggtctc gttgatcatt aactgattaa gcaccatagc ccagttttga
      421 attcgaccac ctgaccactt cgcgtgtaat tctttcgtgc gtaaataaag tagttttagg
      481 agtgcattct cattagagaa cgctcctttt tttgtgactt ttctgaagct ggagtggaca
      541 ctttcaaccg cattggtagt gtacataatt tttcgaatgg cactaccata atcaaatagt
      601 tgttcaacat gtgcaaagtt ccgtttccag acatctacag caccagaata atgagaccac
      661 cgattttgaa aactgtcaaa agcagcatgt gcagcgttta gagaagaagc accgtagaac
      721 tttttcatat ctcggcagac ttccttatag tccttacttg gaatatagcg caatgcattt
      781 cgaacaagat gaacaataca gcgctgaaca attaccgatg gaaagatcgc ttttgcgcct
      841 tcttcgaggc cagaaacacc gtccatcgaa atgaaaaaga catcttcgac accacgtgct
      901 ttcagttcat caaatacttg catccagcga tttttagatt ctgtttgatt taaccataat
      961 cctaaaatct ccttatttcc tttgagatca tagccaagaa tggtgtatac agcatattct
     1021 ttggcttcat aattttctcg taaagtaaca tacatacaat caacgaatag aaaggcataa
     1081 cactttgcta gggggcgggc ttgccattct tccaattcag gaaggacagc gtcagtgata
     1141 tctgaaatca tttcatggga aatatcaaag ccataaatag cttcgacggt tgcggcaata
     1201 tctcgttgac tcattcctcg tgcatacatg gaaagaacct tcccttcgat gtcggagaca
     1261 tctcgttttc tcttaggaat taactctggt tcaaaggaag cttcccggtc tctaggaaca
     1321 tcaatagcta cttcaccaaa actggtttta agcgttttag ttccatagcc atttcgacgg
     1381 ttatcgtgtt ccttaggctc tttagaatgg gcatcataac ctaaatgatt attcaattct
     1441 ccttgaagca ttttttcaaa aaggggccca aacacatctt tcaaagcatc ttgcatgtca
     1501 tcgacagatt caggttgata ggcattcaga atggattcag ctaacttttc ggcatcagga
     1561 tttcttttct ttctagccat cgtgtaatca cctcttgatc ttattgtaga aaaagaaaaa
     1621 ccgcgaagca accacctgcc taggattaat ggttacttac acggtttata ttacactctc
     1681 atcattaaaa aagtatcttt cgatattact aaacaatgaa attatctatt tgaaatttaa
     1741 atctgataat atattgagtg ttccaatgag ggctttgatc ctattatcca tatctttttc
     1801 agttttaata ttgatacaca ttataagaaa aagaaacttt tggttaattt atttcatccc
     1861 agtgcaaaat ataaaaaagt aaaatagacc ataggcaata gattaacaat taatataata
     1921 caagcaaata ttctattcat tgtatttctg ctttcaaaaa gttctaatat tgaaagtata
     1981 attccacaaa tgcctataat aagtatggga a
//
LOCUS       DACDHL010000202         1997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDHL010000202 DACDHL010000000
VERSION     DACDHL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.209"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IV228_002910"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IV228_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8718738.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="IV228_002911"
     CDS             complement(323..892)
                     /locus_tag="IV228_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8718739.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="IV228_002912"
     CDS             1078..1980
                     /locus_tag="IV228_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8718740.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
BASE COUNT          596 a          423 c          333 g          645 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gttttta
//
LOCUS       DACDHL010000203         1975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDHL010000203 DACDHL010000000
VERSION     DACDHL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.210"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1335)
                     /locus_tag="IV228_002913"
     CDS             complement(<1..1335)
                     /locus_tag="IV228_002913"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAP8718741.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNY"
     gene            complement(1394..1855)
                     /locus_tag="IV228_002914"
     CDS             complement(1394..1855)
                     /locus_tag="IV228_002914"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP8718742.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
BASE COUNT          696 a          374 c          243 g          662 t
ORIGIN      
        1 ataattagta cagaattcca acataatttt tgctagaact cccattagca atgcactagc
       61 acaagatttc caaacctgct ttaaaatttt aaatggatta aacttaaaac caaaccataa
      121 aataatccaa aagactataa tctgctctat cttaacgaaa gatctaccat atgctagttc
      181 tataaaccct ttccttgaat agattattag aactggaatt aatactgcta aatgtaggat
      241 agtcataaga acatttgctt ttggttttcc taaaccacga tagtactcag aggcatattg
      301 tcccaagaca agagtaaaac agctcattaa tccatatatc ccaacaaatg gtacagcctc
      361 tttccattga ctcccgagta gaatatttgt tactagttcc ctatatacat atatacctat
      421 acccagagga ataataaaaa tggaaacaat tttaataaaa tctaaatatg tcttatcata
      481 ctccgtttta ctattttgta aacgagataa tgttgaaaat aatacagttg ttgttgctga
      541 agttacaatt gcaaaaatac cattaaccgt tgtcattgta gttttgtata ttcccaaata
      601 gtatacgctt aaaatgcctc ctacgataaa agtacctata taagatgaca accatgtacc
      661 taatgattcc actagtgacc acgcactaaa ggaaaacatt tcgtaaaatt ctttttttga
      721 aaaaaaaagt tttggtttcc actctgataa cttagttaat attgcagctg taactaaatt
      781 tcctattata gatccaatta ctagtgacca ataacttagt ccaattatcg ccaacggtac
      841 tgtaataact aaaggtatca atgcagatat tattctagca taacacaaaa ttttgtaatc
      901 aaaatttcgt ctaaaaattg ccgattgtat actggaaaaa gaagtaattg gaagagaaac
      961 tccagctact ataattacta atccaagtcc agaatttcca actaagtgcg ctattttatt
     1021 attaaacaca caaataaata accataataa taaagaaata cctaagttac tagtaaaagc
     1081 tacatttgtt gatgaaaaca tgtgttcttc attatcaaat tcatgctgta ctaaaaattt
     1141 ttgaaatcct gaatcactaa acatatcagc gaaactaata accatagtta cagtggctac
     1201 tactccaaaa gcatctggag tcaataatct agctaaaatc atattcgaaa ttggagttat
     1261 aatctttgca attatttcag ctaaggatga ccattttaaa gcttttgctg tctttccatg
     1321 aatattattt cccatttatt accccatttt gctaaataaa tatagcaacc tttcgcatat
     1381 taaacattac tttttaatca aatttataat caaatttcaa ctctgttatg attgctggat
     1441 ttccatatac taagctatat ggtggaacac tttttgtaac tacagcattt gcacctatga
     1501 ttgaatcatg tccgatatta attggtccta aaacacatgc accagcgaaa acagccacat
     1561 tttcctctag cgttggtgcc ccaacagttt cttttccatt tattattttg ccatttccac
     1621 ccaaagtcac attttgatat attttacaat tattaccgat ttttgtttta gggtggaata
     1681 cacaaccaag accattatgg actagttgag cattagagct aaaatctacc tgtccgtaga
     1741 attcacatcc ataaataatc cttaaaatat agcaggatat ttttgacatt ttattttgac
     1801 cattcctgta ttgtctacaa gctttatctg cccatttctc tgcttttctt ctcatttttt
     1861 ctacctcaac tttctttttt tatagttctt tctgatgcag aaatcataca caaaacaact
     1921 attgcgtaaa gaaaacgcga agatacaata tccggtataa acattgctcc cacta
//
LOCUS       DACDHL010000204         1966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDHL010000204 DACDHL010000000
VERSION     DACDHL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.211"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..280)
                     /locus_tag="IV228_002915"
     CDS             complement(<1..280)
                     /locus_tag="IV228_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8718743.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAI"
     gene            356..922
                     /locus_tag="IV228_002916"
                     /pseudo
     CDS             356..922
                     /locus_tag="IV228_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1303..1482
                     /locus_tag="IV228_002917"
     CDS             1303..1482
                     /locus_tag="IV228_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718744.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1613..1882
                     /locus_tag="IV228_002918"
     CDS             1613..1882
                     /locus_tag="IV228_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8718745.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1875..>1966
                     /locus_tag="IV228_002919"
     CDS             1875..>1966
                     /locus_tag="IV228_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HAP8718746.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINK"
BASE COUNT          691 a          314 c          385 g          576 t
ORIGIN      
        1 cgatggctcg atacaaataa tgccattttc ctttaatttt gatgtacgtt tcatccattt
       61 tccatgaata aaaggatttt ttatttttct ttttccaaat ttgatagagt agtttgccat
      121 attcttgcac ccaacgataa atcgtcgtat gagaaacgtt aatgccacga tcatataaga
      181 tttcttgaac ttcacgatag ctaaggttat aacgaagata gtagcccacg gctacaataa
      241 tcacatcctg ctgaaattgc tttcctttaa aatgattcat cgtcattcct cctgctatct
      301 ttttctatta ttctacctta tttgatagta gatttaaaac tttgcaacag aacccgaaga
      361 acaattgtat ttggaaagtg aaacgagtca aacgaaggtc acagtcagcc ataatcgttt
      421 agacgtgtat aaacgtcgtc ctgaaacggt aaaaaaagga acggagcttc tacgactaga
      481 aacagaaaaa tacatcatga atgtttacct agaaaggagt gagctggtcg aaaactttgt
      541 cagctacacg atttgcagga aacactatta ctcagaatca gaatattatt caatttttga
      601 gagcatggaa ttaatggaag aagaaatgga agagctaaac agcaaagatg cgacgataga
      661 cgattattac cttgtattta ctagattttt gacgtgtgga acggaatcta tcgtgttaat
      721 gggaatggat tatgaactcg ctgtacccga agcatttttg agggaatatg tagaagaagt
      781 agataaccca ggcgacgact ggtacacgac tttaactgat tttttagaca cctatactta
      841 cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat atcaacgatt
      901 tgaaaggaaa aaaaggcaat aatcatttat accgaaaagt gagaatgtgt gaaataagga
      961 caaaaaacct agtaactaag ggaatggttc ttatttcgca ccaatcgctc caaccgcccc
     1021 cgccgcttag gtggtgcgaa ataagcgggc aacacccgat aacctaagag aaagaaggag
     1081 atccatatga ataaaatcat tcctctattg ttagtcctat ttgttggcga aatacttatg
     1141 cgccatgctg cagctgtgca tttaaacttc gtagtatttt tggcaatact ggtattttgt
     1201 ttgatctttt ggcgctttgt attagccctt gtgagtctac tattcaagat gattgcagcc
     1261 atatttttca tagggttatt tcgatgaaag gagagagtac taatgccaat tgcaatagga
     1321 aataaacggt tacccgttac gttggatgaa aagagacaaa aagaattgca gcaactaaag
     1381 cagaagtacg gcaaaagtga atccaggatt atgtgtattg cgttagattt attgattgcc
     1441 caagaaaaag caggatttga ggtaccagca ctcaaaaagt gacgtcactt tttatcctaa
     1501 aaactaaaag tgatagcact tttaattata agaagttaga atattaatca tttgcttaat
     1561 tgtacaatat aatgtacaat tgttttatag aaataaataa ggggtgaaag gaatggaagc
     1621 agtagcttat tcaaatttcc gccaaaattt acgtagttat atgaaacaag ttaatgagga
     1681 tgctgaaaca cttattgtaa caagtaaaga tgtagaagat acagttgttg tattatcaaa
     1741 aagagattat gattctatgc aagaaacgtt gagaacactt tctaataatt acgtcatgga
     1801 aaaaattcgt cgaggagatg aacaattctc caaaggtgca tttaaaacac atgacttaat
     1861 cgaggttgaa tctgatgatt aaggcttggt ctgatgatgc ttgggatgat tatctttatt
     1921 ggcatgagca aggaaacaaa agcaatataa aaaagattaa caagtt
//
LOCUS       DACDHL010000205         1917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDHL010000205 DACDHL010000000
VERSION     DACDHL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.212"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(323..649)
                     /locus_tag="IV228_002920"
     CDS             complement(323..649)
                     /locus_tag="IV228_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718747.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(636..1424)
                     /locus_tag="IV228_002921"
     CDS             complement(636..1424)
                     /locus_tag="IV228_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8718748.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          596 a          308 c          250 g          763 t
ORIGIN      
        1 gttttgttca aaactatgtt acaatgaatt aaacaaatat atctatccac tatatatttt
       61 tctactgctg ataagtcgcc aaactttgtt aaagcagtag tcttttttta tcttttttta
      121 gtttacctta aaaaataaat taaagcaaac cgctctattt caaggtttaa gctcatagta
      181 actatatcac gatattacaa taatattata tcacgatatc gtaatattac aatatgaaga
      241 taagcgactt atcagctttg atactactga tttttataaa aaaaatatca cgatattatg
      301 atatcgtgat attgaatgta aattaaattt tattttgttt ctttgctagt ttatatcctt
      361 caatcaagta ttcttcaggt atggacttta atagcgcatc ttctataacg tctggtttac
      421 ttttgtctga ttcagcaata gtcgtccaat atttgagtgc tgacattaga attggattta
      481 tacgaatgct atctgctgtt cttacactac ttccttgatc ttttatgact ttacttatat
      541 ttttcttggt taattcgatt tcttctactt cttttgattt aaaattcttt ttatttttat
      601 ttcctagtaa tctacttact gcagcttgac tgcgattatc ttctgccatc aaaatcacca
      661 acttctttta aatattcttc tgtaaatgct ttatagtcat cttcagctgt tgagggttta
      721 ttaaaattga ttagatctga tccagcagct tgcgcttcgt caacggttat tgtcctacga
      781 atataagttt tgaaaatttt tgcaccaatg actctagaaa tttcggttgc tgcagacgtc
      841 atttctttag aaatgttata acgcttatta tacttagtaa atagaactcc tcgtacttca
      901 agcttgtcgt tcgtatattc ttttaccgtt tcaatagttt cacttaactt tattagtcct
      961 tttaatgaga aatacgcagc ttcaactgga atgataattt catcagaaac agtaaatgcg
     1021 ttgactgtaa gcattcctaa acttggtgga caatcaatta taatataatc atagttgtcc
     1081 ttgattggtt gaagtactct tttaagcctg tgttcacttc ctacttgagt aaattccctc
     1141 tctgcagaag aaagggctat gtcggctggc aaaacatcaa ttcctttata gtccagaata
     1201 gcatctgtaa tgtcagcctc cccttttaat acttcataaa ttgtaggttg gtttgttcca
     1261 tctatatcga aacctacatt atctgtagca tttccctgag gatcaatgtc cactaaaagt
     1321 acatttttac cgtaaacaaa tttcaaacat gctgctaaat taacgctagt cgtagtttta
     1381 gaaactccac ctttttgatt ggctactgac aatattcttg ccattaaaat atcccccatt
     1441 catcttatat tataatataa ttatatcata atattgtgat attgcaatat cgtatatgca
     1501 ttatattgta atattgtgtg attattttgt tttaaagata tatatcggtt cggatataaa
     1561 gattattcat atgttattcg aaattaaaat aatcactttt tattacttta aaatttttta
     1621 ttaatgataa gaaatatgtt ttgcgagcag caactagaat aatttaaaca ttactaaact
     1681 gtctatattg ttactaataa ttcccttctt atagtgcatt ttcttagaaa aaaacaatat
     1741 tacgatatca tgatattaag atatcgtaat attatacaat tgtgattgat attatatatg
     1801 tttttttatt tttttatcat gtttaatctt gaaggaatca tattcttttt ctaaagacag
     1861 gagcttttca taaacatcag acatgtttaa actagaggcc aggcttaaat cataaac
//
LOCUS       DACDHL010000206         1858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDHL010000206 DACDHL010000000
VERSION     DACDHL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.213"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="IV228_002922"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="IV228_002922"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8718749.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          417 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagt
//
LOCUS       DACDHL010000207         1821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDHL010000207 DACDHL010000000
VERSION     DACDHL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.214"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            144..542
                     /locus_tag="IV228_002923"
     CDS             144..542
                     /locus_tag="IV228_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8718750.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="IV228_002924"
     CDS             526..1221
                     /locus_tag="IV228_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8718751.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          688 a          267 c          348 g          518 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt g
//
LOCUS       DACDHL010000208         1745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDHL010000208 DACDHL010000000
VERSION     DACDHL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.215"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1050)
                     /locus_tag="IV228_002925"
     CDS             complement(<1..1050)
                     /locus_tag="IV228_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8718752.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQ"
     gene            complement(1152..1505)
                     /gene="tnpB"
                     /locus_tag="IV228_002926"
     CDS             complement(1152..1505)
                     /gene="tnpB"
                     /locus_tag="IV228_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8718753.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(1495..1689)
                     /locus_tag="IV228_002927"
     CDS             complement(1495..1689)
                     /locus_tag="IV228_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718754.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          485 a          362 c          312 g          586 t
ORIGIN      
        1 ttgagctgcg ggaatatccg tgtttttctt tccttttgga attgcttcga aaaatttccg
       61 ccgaacatgc gcgaaacaat agacaagtcg tacactttcc acggcattat acccgctata
      121 gccgtcacag tgtaaatacc ctgtaaactc cttgagaaaa gttttcggaa catctccagc
      181 acgactttcg tcgtggtgat aataaacgat gggatgctga ctgtcttttc ctgtacaaaa
      241 ctgccagtaa taggtatctg ttttggcact ttcaatcaca cgataacttg tttcatcccc
      301 gtgtagaatc tcttcttgat taagatagac tcttaatcgt tctcctaaag ggtctaacgc
      361 gttttgcact gctttaatat gccaattggt gatattatct ctcgaaatat ccaacccatg
      421 tgtttgtttc caatagttct cttgccgata ggcaggaact ttctgaccaa atttcatccg
      481 gatcgtttcc gccactaaac tattggaacc taagctatta gaaatcaaag gtttaggaac
      541 gatcgctttt ttaaaagaag aaagtccttc tttttcgcag cttttacaac aataggtatg
      601 ttgataatgg gtgagacaat ccaacatagc ctgatggaaa cgaatttctt ctcgtacttt
      661 tgtccgtccg atatctttca tctgtgaacc gcaatgttca caaaattgtt cttccaaagg
      721 aaggagatgt tctttctctg ttataggtaa gtgtttgatt ttgtctgctt tcgttcctac
      781 aactcgttga tgtcctttta ctggaataat ggtggcagct gcttctggaa cacttacaga
      841 aatttcttca tcactaaaaa gattcaattg cccattcgtt tcctcaaaga gagtctcttt
      901 agagcgtcca aataatttgt tggttaaata attgaccttt tcattcaata gttggatttc
      961 agttgtaaga ttctgaatgg tttgttgttg tgcttgattg gttttatggg cttcttgaag
     1021 caactgaagc aacgtatctg tagtttccaa aatttttcac caccttgaat cgaactgatt
     1081 ttgttacaga ataccacaag aaacgcacaa gaaatagggg ttcaacacat tttttgtgtt
     1141 aaccccccag cttaaaaaac tccttttttt cctggcaaaa tagctcgagg ttgatcgata
     1201 cttaatcctt ctaacaacca tcgaacctgt tggttcgtta aggttcgaac ttcgtctttc
     1261 gtccgaggcc attttaactt gccgttttct atccgcttat aacagagaag aaaaccatca
     1321 ccgtcccaat agagaagttt gaaacggtct tgacggttcc cgcaaaataa aaaaacggca
     1381 tcttcgtaaa gctcgagttg atactcttgt tgaataagac tggcaagacc atcaattcct
     1441 tttcgcaaat ctgtttttcc gcacacaata taaatatgtt tggtttgaag taaatcataa
     1501 agcattggac aacagttctt tcaaaatagc ttgaagaata taattgttaa taccgttata
     1561 gatcaaaact tcagaaccat tctctctttt gatgcgcatc gctaacttgg cagccgcggg
     1621 tttacctttt tctggtgcac tcttttcgtt tcctaattgg acaggaacaa tctcatttaa
     1681 tttatacata gaaaacctcc agacttcttg atagctcaag tatatcttga ggtgcatttg
     1741 ttagt
//
LOCUS       DACDHL010000209         1736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDHL010000209 DACDHL010000000
VERSION     DACDHL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.216"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(129..1169)
                     /locus_tag="IV228_002928"
     CDS             complement(129..1169)
                     /locus_tag="IV228_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8718755.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          581 a          262 c          216 g          677 t
ORIGIN      
        1 atattttaac tcataaattc tattaaaata gctatttctt tgtaacattt gtaatataat
       61 tagaataata aaaaattttt aattttaatg ctactccatg aagcaacaat caatttgtca
      121 gtgaaatttc actctaacca gtctttcgta atagtttttg ggaagctttc agaattcaaa
      181 cgtttttcac ttgatacttt agcagctatt ggtttttcga taaaccaatt ttttacatag
      241 acaaaaatca ggttctggat attttcaact ttttcggttt ttaatttttg ataggctttt
      301 aacagtgtcg tgtataattc ttgttctgca ttgacataat aactatcgag ttcttcaaaa
      361 attattgaaa taccactttc tgtttcggct tttttctttg cattatgaat agtctggaca
      421 gttttttgag cttcactgta acttgaagaa aatgttttta ttaaatttaa tactcgttca
      481 gggataaacg tagcaatgct gtcgtttgtc ataattgtct caaaattgtc taacaacaat
      541 tgatcttgta ggcggtcttt ttcagtgtct atattgtgtc tattagtgtc taatatatta
      601 ttattaagtt ctggtatgat tttcataccc tctgatttat caacgttttg gcacggtatg
      661 attttcatac cctcttgagc cccaagggat tcagcgtctg atttttcgtc gagggtgggt
      721 atgattttca taccctctga tttatcaacg ttttccctat tctctctatc aaatttttcg
      781 agtaagtaga tatcattttc tgagactttt aattctgcta gatatagtct attaggatta
      841 tttttcccta actgtttgtt aaatcccatc tgcttttgta ataataaacc aaaggattcc
      901 aactcttttt tgattgctat cgctttttgt ttttggcagt ttaatacgct acatagttca
      961 tttgtcgtat aagtaaaata aacattacct tcttcatcga tttgccgttt gctgattgcg
     1021 atctctaatc ttgcttttaa aagcatatag gcaatcttag ttgaatcttt catttttttg
     1081 tattcgtcag aaacaataaa gacttttgga aattgataaa ataattcgtt gtaaactcta
     1141 tccgccttaa aaaaattaaa atcagtcatt gtagatcctc tactttcacg ttgttttatt
     1201 tatgtaaaat tacctactct tttcgaaaat agcaaagata aataaaaaat gcaaacgcat
     1261 taaaactgtt ttttaattaa atactaccta aaatcagaaa taaaatgaat tatgtactac
     1321 ctttaaagtc attaagaact actttaaata aatattattt ataattaaat actaccagaa
     1381 gaacgtatga ataaaattta gttttaaaat taaagataat taagtactac cagtgtcaaa
     1441 acaaaaaata aattaagaac taccttatgc atataaaaca tactaccatt actagtaata
     1501 tatactacct tatttcagta gtatatatta tattcttctt ataagacaag gctttaggct
     1561 agaaaacaat agattatata ctactaaaaa atacaataag tactaccaat gtcagatata
     1621 tataataatt atgtactacc attaaaatta acttatttaa taagatacta ctgtgttaat
     1681 ctgaaaaaaa ttactttaaa gtagttacat actacctgtt aaattagata ctacca
//
LOCUS       DACDHL010000210         1730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDHL010000210 DACDHL010000000
VERSION     DACDHL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.217"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IV228_002929"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IV228_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8718756.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /locus_tag="IV228_002930"
     CDS             complement(1305..1709)
                     /locus_tag="IV228_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP8718757.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          510 a          300 c          273 g          647 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg
//
LOCUS       DACDHL010000211         1689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDHL010000211 DACDHL010000000
VERSION     DACDHL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.218"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..346
                     /locus_tag="IV228_002931"
     CDS             86..346
                     /locus_tag="IV228_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718758.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(708..947)
                     /locus_tag="IV228_002932"
     CDS             complement(708..947)
                     /locus_tag="IV228_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718759.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1233..1547
                     /locus_tag="IV228_002933"
     CDS             1233..1547
                     /locus_tag="IV228_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718760.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          585 a          225 c          291 g          588 t
ORIGIN      
        1 atcacaagtg ttattgattt tcataatcat attttaatgt tattatcatt ttaaataatg
       61 aaaaaacatt aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata
      121 tatttgccaa cctattggct tgaaacaaaa agtgattgga gaagtaatca gtaaattaga
      181 aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga
      241 aaaatgcgga aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata
      301 tctatataac tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag
      361 tgccttcaaa agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg
      421 ttcatgattt ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc
      481 tggtggagaa actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa
      541 actagtagtt ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc
      601 aggatcattt agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg
      661 agtaaacgcg aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt
      721 tgtttgaaat aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa
      781 acttttatat cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg
      841 atactattcg aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca
      901 gtaagtaaca ataaatttga taaactgata aacagaattc ttttcattat gtacacactc
      961 cttatagtat cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta
     1021 tcaagtcttt tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc
     1081 agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt
     1141 tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt
     1201 gggtatttaa atagttgcta ggaggatagc ttttggattt ttacagtaat tttatcttaa
     1261 taatagcaat attattactt ctaaatattt ggttttttga taagagtaga aatgcaggga
     1321 taggttttag aaccaaacga agtacgtctt ctgaaaaaaa gtgggtatat tcacagacta
     1381 ttttttacgg aggggttatt tcaatcagtt tactttcttc aacactttat tcttttaatg
     1441 ttatagacgt ttccatgtca aattttatat ctattattgg aatccttatt tctgcaatta
     1501 ttacacaact acttcttgtg tttgaagaga agtcaaaaaa taattaaagc aattatgcct
     1561 tttagtacag gaattattac ttcttacaaa tgtatagcga atgtgaagcc tgctgtttta
     1621 attgttctag aaaaactttt gagtgaacaa ttattttatg ttaggatgaa ttaatttctc
     1681 tagagttta
//
LOCUS       DACDHL010000212         1670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDHL010000212 DACDHL010000000
VERSION     DACDHL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.219"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(260..457)
                     /locus_tag="IV228_002934"
     CDS             complement(260..457)
                     /locus_tag="IV228_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718761.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(533..733)
                     /locus_tag="IV228_002935"
     CDS             complement(533..733)
                     /locus_tag="IV228_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8718762.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(853..>1670)
                     /locus_tag="IV228_002936"
     CDS             complement(853..>1670)
                     /locus_tag="IV228_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8718763.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
BASE COUNT          487 a          341 c          292 g          550 t
ORIGIN      
        1 tttgcccaca cctttgttat gccaaattgt taggctgtct gatttccatc cagccattct
       61 ttagccagcg atctacctca tcaaaacaaa aaattttcct ctgatggttc cttaaataag
      121 actattttga tcctttttcc atgaataaga ttttgcatca gacaatactt catttctgaa
      181 aaagttgcgg caccaatata gcctaatacc cctttccgat cttgattaaa tacaaaaaat
      241 aaatccgctg ctgcgatatt taaaaaaaag tcgtcaagat ttctggatac tctttgtcta
      301 ggatcggaca tgagcgagga taatcaatta attcaatgcc ttttttactg aaatcttttt
      361 ccatatctcg atattcttct tggaaaacaa tacttccaga aataacagct ttcacaaaat
      421 tggcctccat ttttttattt ttcagacact ccaccatacc ggatacctct caaagaaaag
      481 acatagcaaa aatcaatagc agctatctct ccacggtttt ttactggtaa ttttattttt
      541 cttttggaaa ttgtatcagg aaggtcgttc cttcattttt tcggctggtc acttcaattg
      601 aaccgtggtg caggcgcact agttccttca cgattgataa tccaagacct gactcgccta
      661 tctttgtatt tttacttgac ggatcggctt tataatagcg atcccaaatg tttttttgtt
      721 cctcttcaat catgccgatg ccattgtcct cgacagtaat aaatacaggc tctttgtcaa
      781 atgatgttgg taaagagaaa tcttagaata atttggacag aacagaaaga agaaactctc
      841 tttctgttct gtttatttgg ttaaaaatgt tttgccatga cgaatcatga tccgtagttt
      901 gaaattgtaa aagctgcgaa agccgtacgc attgcgtttt aatactttga tgtggttgtt
      961 taaacattcc aatggtccat ttgaatagct gtagtgaagc gcattttgga tctgtggcag
     1021 aaacttttta aacgttcgta aagtggttgt ataggtttca ggaagaccgc tgacttctct
     1081 gtttaaacag tcctcaaata atgactgatc tcttttcttt accgcataaa gaatgtcttg
     1141 gtataactga tagccttggc gcaggttggg acaagcagat aacagtcgat cgacaatctc
     1201 actttcagtc aaataatctt taaaactggc acgccaaacc cttttttcgt agtttaactt
     1261 tgcttcttct ttttgtagga gtttccaata gcgtttcaat tttttcccta accctcgttg
     1321 agagtgggaa gttcctttaa gccgtgccgt ggtttctttc actcgatgat tcaagaaggt
     1381 tctgccaata tgttggacga tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag
     1441 ctttttcacc aatgaaacat aaggcgcata catatcaata acaatccatt gaacggcttc
     1501 acgagtcgct aatgaaaacc gagaaaaata gcattctaaa taaggcaagc gtcgattttc
     1561 gacaatatcc aataatgcat ggctttgtcc gtccatcatg ataaaactca ttgcactggc
     1621 aacacttcga accgatttga attcatcaaa gcataagacc tttggcaaag
//
LOCUS       DACDHL010000213         1645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDHL010000213 DACDHL010000000
VERSION     DACDHL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.220"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(620..805)
                     /locus_tag="IV228_002937"
     CDS             complement(620..805)
                     /locus_tag="IV228_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718764.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT          569 a          282 c          224 g          570 t
ORIGIN      
        1 aatctttatt tattagatat tcaacagaaa tcccgataac actagccaac aatatattta
       61 tcagaagtgt aaaataagga tgctttttat agaatagaac tagtgttgtt ttcatagact
      121 ctcctttctt catatttcca attaatcaca aattcacggc attactagaa aaattaaagg
      181 ggcgcagaag tggaaaaata ctcttcgact ttccctacaa accaggccat cttgtgttta
      241 ataactatac caaatctaag gtacctctaa atctaaattc catttgtggc ggtgacctcc
      301 aatttttaac tcttctatct cccatccagt atacataacc ttgatacttc gaccacttat
      361 atatcgaaca cacaaatacc tatccattgc aggagtacta cctaacttag ttgctacttg
      421 catctaaata acagctgaaa agataggact tactcttagt aaagtatctg aactattaat
      481 ttttaaacta tcatttttat cctcaactac atgcacatct gttgcaactt cactttctgt
      541 ctcatccaga taaatgatta aggaatcggg aattgcaata tcttcagctg acgctggaat
      601 agttgagcta aattagtcat catatcttta ttataaaata accaacagca aaaaatacaa
      661 tgaaaaacaa acttggaact aatgtatcat taataaatgt tgaaaaattc agcaactcat
      721 tactgtatga ttttagcagc ccaaccaaca tataccctat aaaagctaat aagatggtta
      781 ggaatccttt agaattttta ctcattcata gacacttcct taaataactt ttattataga
      841 aggtattgta aataataata ttgacaaaaa gtgactcaaa ataaaaaaaa ccttacatta
      901 tactggaatg aactgtattg atagtgtacc taaaagctct actgacataa gatcgtgtaa
      961 tcggtaaagt aattttatta attatacggg atgtaatcaa ttagagaata aactaatcat
     1021 aaaaggtact tttacattca taattgtcgc tttgagattc tactttggtt cactatctga
     1081 cccaattgca cattttcgat cattacaagt attggtgtcg ttagaacaac taaataaaaa
     1141 ctagcggtag atttttcaga atctatgcta atttctgtat ggatgttcca gtgatggcaa
     1201 atttctcttt tcattgtttt tcttgtcgca tgatatgcct ttttgtgtta ttcagttaaa
     1261 ctatagaaat tctttcctag acagatcttg atcccaaatt ttacgttttt gagcatcgat
     1321 atagtttaaa atgacattac tcatatctaa agagtttaga ttttttagga ctaaaactgt
     1381 cagttgttct ttttcttctt tctgttaaag tattgtcgcg acttttcgta tacgttaaga
     1441 tatgtcgtga aaaaacaagt tttgtgacac aaaaaaacga atgacttgag gaaatcacct
     1501 caatcattcg tttaaaatgc tatatattct ttagtttaag atacggaaat taatgccatc
     1561 gaccacacta gtagaatgaa cgacatttac ttggcctttt tttaatttcg ttatgtttag
     1621 catataaaaa tattatagag tttac
//
LOCUS       DACDHL010000214         1640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDHL010000214 DACDHL010000000
VERSION     DACDHL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.221"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..909
                     /locus_tag="IV228_002938"
     CDS             <1..909
                     /locus_tag="IV228_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP8718765.1"
                     /translation="RRKKLAQMIQENIKEELGLIATVGVGDNPLLAKLALDNEAKHNE
                     GFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRL
                     GVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQV
                     AIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEG
                     QEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSM
                     LEGARSITRSTLVGGHAGGNGGIKND"
     gene            902..1252
                     /locus_tag="IV228_002939"
     CDS             902..1252
                     /locus_tag="IV228_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718766.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1249..1428
                     /locus_tag="IV228_002940"
     CDS             1249..1428
                     /locus_tag="IV228_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718767.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
BASE COUNT          576 a          253 c          359 g          452 t
ORIGIN      
        1 cggagaaaga agctggctca aatgatccag gagaatatta aagaagagct aggattgatt
       61 gctacagtag gtgtcggaga taatcccctt ttggctaaac tagccttaga caacgaagca
      121 aagcataatg aaggatttgt tgcggaatgg acgtacgaaa acgttcctga aaaagtatgg
      181 aatattcctg aaatgacgga cttttgggga attggctctc gaatgaaaaa aagactcaat
      241 cagatgggga ttatgagcat tagagatctt gctaactgga atccttatac gattaagaat
      301 agactaggtg tgattggctt acagctgtac ttccatgcta atgggatcga tcgaacagat
      361 atagcaatcc caccagaacc gacaaaggaa aaatcatatg gaaattcaca agtgttacca
      421 cgagattata ctagaagaaa tgaaatcgag ctggtggtaa aagaaatggc ggaacaagta
      481 gcgatacgca ttaggcagca taactgtaaa acaggttgtg tccatttaca cattggcacg
      541 tcgatcttag aaacgagacc agggttttca caccagatga agatccctat cacagataat
      601 acaaaggaat tgcagaatta ttgtcttttc ttgtttgata agtattatga aggacaagaa
      661 gtaaggcatg ttggaataac ctatagcaag cttgtgtaca ccgattcgct tcagctggat
      721 ttatttagtg atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag
      781 attcggcaaa aatatggttt tacgtcgatt gtacatgctt cttcaatgtt agaaggcgct
      841 agaagcatta ctagaagcac acttgttggt ggtcatgcgg gcggaaatgg gggaattaaa
      901 aatgattgat tacaatgatc gtggacgttt aaagtgggca gggttttatt tatctgatca
      961 tactgaagaa attgatgccg acaatgaaca acgaaccagt caaaatttag caaaagaaca
     1021 aatgacgact gaagagattg cggaagtatt gaacgctgca gtcataaaaa atagatcagt
     1081 atctattcaa aaagaagaac gaaacgctga aggatactat ccagaagata tcattggcaa
     1141 gatcttaggt agtgatgagc tggggatcta cgttggaaaa gacaaaattc actacgacga
     1201 aatacgtcat atcgaatttt accaggataa taaatggagt cgattagaat gaacattatc
     1261 cagcaatatg agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat
     1321 ggccaacgtt tgataaatga ggttggtgtt gagcgcttct ctttctatgt agaagctgca
     1381 gcaggttatc ataattttag gttttatatc ttcccgttat atatataagg tattttacgg
     1441 attctataga gttatctcta tgaatccttt tttgttttta caaataggag tagatactag
     1501 aataatgcgt gtgttaaact attattgcaa acgcaataat aaaagaaagt aggcttacgc
     1561 accatgtcta tcaatacatt tgtttactcg cacccgatca atgtttacat tatcaagaat
     1621 ttaggaatca ctgtagaaca
//
LOCUS       DACDHL010000215         1592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDHL010000215 DACDHL010000000
VERSION     DACDHL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.222"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            52..1035
                     /locus_tag="IV228_002941"
     CDS             52..1035
                     /locus_tag="IV228_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8718768.1"
                     /translation="MPKVFFTNEKYMDLSNDAKIAYMLLKDRFDYSVKNNWVDSDDNI
                     FFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVNKRDGKIESMPNLLYLGK
                     PEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTTVIAKIANTAKSSHIKDSTV
                     IAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMKEDSINTFVPENVLNLI
                     KTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELESYGIDADKGLYMTLLKAYQK
                     QKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT          614 a          232 c          267 g          479 t
ORIGIN      
        1 gattttaatt attacaaatc aaaagaaatt tatcgtgaaa aatattatca aatgcctaag
       61 gtatttttta ctaacgaaaa atacatggat ctatcaaatg atgcaaaaat tgcttatatg
      121 ctacttaaag atcgatttga ttattcagtc aaaaataact gggtagattc tgatgataat
      181 atatttttca tattcacagt tgaagaatta atgaaattgt tacaatgccg cgaaggtaaa
      241 gtatctaaaa tcaaaaagga attagaaaaa gcaggtcttc tgaaacagaa gagaggaaga
      301 gtaaataaac gtgacggtaa gattgaatcc atgcctaatt tattgtactt aggcaagcca
      361 gaagtaacga atcaagacgt ctttaaaatc atggaagagg aagacaatac tgataccacc
      421 gtaattgcga aaatcgcaaa tacggcaaaa ccgcgagaaa tcaatgatac caccgtaatt
      481 gcgaaaatcg caaatacggc aaaatccagt catatcaagg actcaaccgt aattgcgaaa
      541 atcgcagaca atctattcta ttctaattct atagacacta atagacacat aatagacact
      601 gaaactgatc agttacaaaa tcaagtttta ctagataact tcgtcgacat catgaaagag
      661 gacagtataa atacatttgt accagaaaat gtattaaact tgataaaaac attttctagt
      721 acttacgaag acgctcaaaa aaccgtccaa actattcata atgcaaagaa aaaagctgaa
      781 gaattagagc aaacagttgt tgtttatgaa gaactggaat cttatggaat tgatgctgac
      841 aaaggattat atatgacttt gctgaaagct tatcaaaaac aaaaaactga aaaagtaaac
      901 aatttgcaga atctaatttt cgtgtatgta aaaaattggt ttgtcgaaaa agctattgcg
      961 gcaaagcttg caaacgaaca aagagaaaca tttaacgaat taccaacagt tagtacggaa
     1021 aattggctag agtagttgat aaaaagcgat cactataaaa atgatcaatt ttaaattaga
     1081 acttttttat gattttaatt ttattacaaa tgttacaaat aaatagctat tattccggag
     1141 tttttagatt aaaagtttat gaaaacaatc acaaacgtag gttttaaagt cataatcgtt
     1201 agatatttta gttatttttc attcaaaaaa atagggaact agtgtttgca tatgtgcttt
     1261 tttgagttat aataattcat gaagagaggt aattcactta tttccccaag ctgaaaagcc
     1321 tgatattact tcttttcagg cagtgctgct aatattcaat tacacttttt tgtatatttt
     1381 tagttgtttt aaatttaatt gtctgatatg cttgtgatga attgattttc gttctgcact
     1441 tttggacgat aaattgtgat aattgaaaat acaaaaaaaa gaaaggcccc tgtaaggctc
     1501 gggagactgg taatctccgt cagtgtccta caggaaagca ttaagatgac gccttaatgc
     1561 gtgcccttca gtgttacggt attacccgca ac
//
LOCUS       DACDHL010000216         1574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDHL010000216 DACDHL010000000
VERSION     DACDHL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.223"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            45..995
                     /locus_tag="IV228_002942"
     CDS             45..995
                     /locus_tag="IV228_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAP8718769.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(992..>1574)
                     /locus_tag="IV228_002943"
     CDS             complement(992..>1574)
                     /locus_tag="IV228_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718770.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT          542 a          238 c          301 g          493 t
ORIGIN      
        1 tataattgta atgtattacc aaatagttag cacttagatt agtgatggaa aggaatagtg
       61 agaacaagat gactggtaga aatcccaaag tatttatatc atattcatgg gagaataagg
      121 aaaataaaga atgggctaga gaactagcag acaaattact tgaaaacggt attgatgcat
      181 atatagatca gtatgattta agtttaggtg atagattacc tcagtttatg gagcagcaaa
      241 tatccgattc agattatgtt ctaattatat gtacaccaga gtataaaaaa aaggcaaata
      301 atcgtacaag tggtgttggg tatgaaggtc atattatctc agcagaattg ctttctggaa
      361 aaaatgaacg taaatttatc ccaataatta aagaaggacg tgcaagcgaa gtacttccta
      421 cttttcttgc tggaaagctt ggaattgatt tatcagatag ccagcattat gatgaaaact
      481 tagatgattt aattacaact atatttggtg ttaagagaaa accagcatta ggtgatatac
      541 ctaggcatat taaaggcagt cctttactac ctatagaaca taatgaaaat aaagatattg
      601 aaatcttagg aattatcact gataaagtaa ctgtacctaa aatggatggc tcaaggggtt
      661 ctgcattata taaaattcca tttagattaa atagagtacc aagtcagctt tggaaagagt
      721 tgtttgttag aaattggaat tcaccaccac gatatacaac aatgcataga ccaggaattg
      781 cttctgttgt agacgatgaa attttactta atggaacaac tattgaggaa gtccgggaat
      841 atcatcggga aactttaaaa ctttgcattg aaaaaagtaa tactcaagaa aaaaaaatat
      901 tgctagagga ggaacaaaaa aaagagagag agaatgagcg agtcagatca cataaagaaa
      961 gtgttcagaa attagcagat gaattagagt tctagttgat agtcagttct ttcttaattc
     1021 gatacacagt ttgtctagtt acattatagt ctgttgctat ttttgaaata gcaacaccat
     1081 tctcctggtc ctgaacgatg cttctataaa caaggcgacg ttgaggatct tttgtgttcg
     1141 cactgtataa cttaggacgt cctttataga cgccgtttgc tttagcaatc tttatccctt
     1201 gtgcctggcg tcgttttgat tcagtacgct cttgttcagc aatcatggcc aagatttgaa
     1261 tgattaggtc tttgataaat ttatccagga gaggatttcc gattgcttca gccatgatcg
     1321 gtaaattagt gataataagt cgcacatttt tcttctttag ataatttact gaatcgataa
     1381 tctcgtcata attacgtcca agtcgatcga tagcctctac aataaaaata tcttggtctc
     1441 taacaaaatc tagaacttcc tgaaaaatcg gtcgatgagt aatagtcgct ccagattgtt
     1501 tctcgatgaa aattttttca gcaccaaatt ttctcatttc ctcgatttga cgttgttcat
     1561 tttgatcgat cgat
//
LOCUS       DACDHL010000217         1511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDHL010000217 DACDHL010000000
VERSION     DACDHL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.224"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            337..1422
                     /gene="xerS"
                     /locus_tag="IV228_002944"
     CDS             337..1422
                     /gene="xerS"
                     /locus_tag="IV228_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8718771.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          580 a          256 c          209 g          466 t
ORIGIN      
        1 tctttaactt ctccctcccc ttttttattt gtatatacat tcatatacgc ttgttaatat
       61 caaatcaacc gtagtctctc ttatgtctaa ttatatagaa agattgttct ttctatataa
      121 tttagacaat ttaaaaaatt aagattgcct ccttctgttt cctttcctat tcatgtatac
      181 catttatttt aaattattaa atctatacct atttatctgc ttttttgaaa taaattaata
      241 aatttcagag tataccaatt gacaaaacac tctataattg gtatagtcca attatagaga
      301 tactcctctc ccctacttat cgaaaggatg aataacatga aaaatacaga aatatatcaa
      361 aaaatagata caatcatact tcatatgccc gattatataa aagattttgt tcaagatcgt
      421 gaggataaag atcaatcacc tcgaactact ttagaatatc taaaaaatta taagctcttt
      481 tatgaatggc ttctgagtga gtctatcgtt cccgatactt caatcacctc gataagacag
      541 ttaactgcct atgatctaaa cttgtacaaa tctcatttga aacgtcgcac aaaggaaaat
      601 gtaaaaaaag agacaacaaa aactaagctc aaagataata ataacttagg tctatctact
      661 tctactatca atagaaatat cacagcatta aaggttctat ttaaatatct ttcgaaaagt
      721 tccaataatc ctttaggaaa accatactta gaaaataatc ctatggatca agtagcaact
      781 atcacagata aaactacgtt agctgcccgt gcaaacgcaa tcgagaaaaa attatttcta
      841 gacgaagata cacaaaatta tctagattat attgccaatg aatataaaaa aactttgagc
      901 aaacgagctt tgatctatta tcaacgagat gtagaaagag acctggcgat caatgcattg
      961 attttaggat ctggattaag actctccgag gttgtaaata ttaatttaga ggacctatct
     1021 ttagaaaaga ataatgtggt agtaactaga aaaggaaata agagagacgc agtaaatata
     1081 gcagcatttg caatggaata tttagctaat tatctagcta ttcggaaaga acgatacaaa
     1141 gtaacagaaa acgaaaaagc tttatttcta gcaatttatc aaggagaagc caagcgtata
     1201 tccggtattg caatcgaacg gatggtagca aaatattcaa aaggattcag agtccaagtc
     1261 agtccacata aattacgtca tactttggct actagactct atcaacagac aaattcattg
     1321 gtattaactg cccaacaatt gggacataca agtacaaata caacaacttt atatactcat
     1381 atagacaatg cagctacaat tgatgctttg aacgctttgt aaatgtagta aaaaatagta
     1441 taataatgaa gcctgtctct aaaattagat aaaagcccaa ctttaaaggc aagcttcatt
     1501 atcattttta t
//
LOCUS       DACDHL010000218         1446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDHL010000218 DACDHL010000000
VERSION     DACDHL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.225"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            835..1047
                     /locus_tag="IV228_002945"
     CDS             835..1047
                     /locus_tag="IV228_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8718772.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1037..1432
                     /locus_tag="IV228_002946"
     CDS             1037..1432
                     /locus_tag="IV228_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718773.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          500 a          215 c          243 g          488 t
ORIGIN      
        1 tactctagta tttgtgttac ccgaataatt acttgattat attggtttcc catataattg
       61 aaggctgtcc taatttttac ctactatatg atcataattg attaactctt atatatttcc
      121 gaaaattatc ttgaagattt ctattggtat ggagaatatc catttcattg ccaataagga
      181 tataaattct caaaataaca atcgatgtct gtattaaaca agcctagaat ggtaaatctg
      241 attcgccaaa accattggat tgtatcgatt tgctttacgc gaacgccacc tacccatttt
      301 ataaaaataa aatgggcaaa agatctttct ttttgtcata caaaaagaag aaaaaaaaga
      361 gacgattttc ttcttccaaa atcgtccctc ttttttctaa aaccatggat gaacgaacaa
      421 aaaatctctc gtattttgtt tgttcatcca ctaaaaatat gcgaacagta tggtttcatt
      481 caatcctgtt cttacaagtc aaaagcatcg aatcgtcctt ggacgattca acaaaaaaag
      541 tagtcgtttt tggatgacta ctaagttcta aacattcatt acccaaaatc aatttggtct
      601 aacatcgttt ctcgactggc ttgatctaat cggctcttga cagggctttt tcttttctgt
      661 tgatagcaga taagaggaaa taagaataag tgtgttcatg tgtcataaaa acagtaacta
      721 aaacattaaa atcaaaattt atatggaaag cttccttgac attttgtgct cgcaggagta
      781 ttataaaagc acgaaaggaa agtaaacttt ccatttttta gaaaggagca tgttgtgaaa
      841 aatcgtttgg aagaattacg aaaacaaaga ggaattaaac aagaagattt agcttctgct
      901 cttgaagtat ctcgtcaaac aataggttct ttagaaaatg gacgttataa tccctctatt
      961 atattggcat ttaaaattgc aaggtacttt caaatgagca ttgaagaaat ttttatctat
     1021 gaggaggaaa gtgaagatga aaaataaggg tcagatttgg tatatcggtt atattgtttc
     1081 gttgtttttg attgtgacaa ttttggttac agattttcct aaaatggtag atattggact
     1141 tgctatttta ttcagtgccg tttttggcgt ttctcatgtt caaattttgc atgataaaat
     1201 gattaaaaat gatgcagact acaaaatcaa tgttatggac gaacggaata tcataatcaa
     1261 agaaaaagcg ggaaatataa caaatatgtt taacacaata ctgttagggg tggcaacagt
     1321 tatatttatt tgtttagact acatcattcc agctattatt gtcggaagta tcattgcaat
     1381 ccagccgatt attttaatca ttatcagtag tgtgcttgag aaaaaaatgt agaaataaag
     1441 gttcat
//
LOCUS       DACDHL010000219         1442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDHL010000219 DACDHL010000000
VERSION     DACDHL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.226"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..765)
                     /locus_tag="IV228_002947"
     CDS             complement(<1..765)
                     /locus_tag="IV228_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718774.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLENINETVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGE"
     gene            1305..>1442
                     /locus_tag="IV228_002948"
     CDS             1305..>1442
                     /locus_tag="IV228_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8718775.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FK"
BASE COUNT          482 a          261 c          158 g          541 t
ORIGIN      
        1 ttcaccttca atctggggag catatagtcc aatttcctcc caataatatg aatctctttc
       61 aattcccatg atggtagctt tggctaattt caaatcaaat aacgatttta gaattcttag
      121 tgtctcaata aagtcttctc tcttcaactc tttaatccat tgtgaatttt ctggattatc
      181 taaaatttca agttcatctt cattataagt atatttcaaa tttgacttgc ttaactcata
      241 gataaatgaa gtttcttgac tcataattct tttaagtact tgactataag tttcgtttga
      301 ctttgcccat ttcgctaatt ctaccactgt attcactaat aaatcgaaat aatgatgttc
      361 tcgttcaata gtctcagcat tttgaaaatg aaaatcacct ttataaactc cattcttttc
      421 cactgtttca ttaatatttt ctaaaaacca gaatgagttt gcaaatttag tcgtactttt
      481 tcgattaact aagttaataa ctcttccatg taataataag acaaaaatac ataaattttt
      541 agcttcctta ttattagata ctaattgatc aatcataggg agccattttt tgattttcac
      601 taattgattg ctcataaatc tttcactgga cttttgttca atcttaaatt gcaaaacttc
      661 taattcataa tttccatctt ctgatttatt tttgggaaca actatcaaac ttttataaag
      721 tttatccaca tcaaaattta tcttttttgc gtaatttgta gccattaata catctcctta
      781 ttcataataa atttcatatg acgctcctct caaaatttac cggtagacta gaaaaaagaa
      841 taaaagatac gtcaattcac actttattct taaaaaaaac tggtctctct accggtaata
      901 tcgattgagt ccgcctttat cgactttatc ctttttttat attaaatgtt ttcaccggta
      961 gacagggaga gaaagaataa actaaaagcc cttaattctt aatgttttca cctccttacc
     1021 gttcctaccg ctaaaattta atataactaa attctataac attagaagaa attttaaata
     1081 cctttgataa aaaatattga tgttttttct ctcattgttt acaaactaga tagcttatgt
     1141 attcttatta aaaataatag atatttcgaa catcatcact ctcatctact taatttcgca
     1201 tttttacttt acgccgattg taaaattaag ctagacaact aaaaaagtca tttgtgctac
     1261 actcaaaata gttccgacca aagaattata aggagtgagt acaaatgacc tatacccatc
     1321 ttacaacgga tgaacttgta ataatagagt cttatttcaa aattaatcaa tctgttgcta
     1381 aaactgccca ttgcttgaat cgttcaagac aaacgatcca taaagtatat ctgttcttca
     1441 ag
//
LOCUS       DACDHL010000220         1411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDHL010000220 DACDHL010000000
VERSION     DACDHL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.227"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            88..1380
                     /locus_tag="IV228_002949"
     CDS             88..1380
                     /locus_tag="IV228_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8718776.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          459 a          287 c          256 g          409 t
ORIGIN      
        1 cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga attaatttgg
       61 cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa agaaacatta
      121 gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa
      181 gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga atgcatccac
      241 tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt ccggttgtta
      301 atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc
      361 aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca ttgtttcatc
      421 agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag
      481 atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa gcgattttat
      541 cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga tgaattcaaa
      601 tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca aacacagcgc
      661 ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt tagtcatttt
      721 tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc tccttatgta
      781 tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt tcacatcgtc
      841 caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca actattaaaa
      901 agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg gaaacttctt
      961 caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg cttcaaagct
     1021 catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta
     1081 ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta cgtatcgttt
     1141 gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac cttgagaacg
     1201 ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc aaatggccca
     1261 cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc
     1321 ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat tttcaactaa
     1381 aaaagatcaa gaggaactga tccacttgat c
//
LOCUS       DACDHL010000221         1383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDHL010000221 DACDHL010000000
VERSION     DACDHL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.228"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            97..654
                     /locus_tag="IV228_002950"
     CDS             97..654
                     /locus_tag="IV228_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAP8718777.1"
                     /translation="MSIINTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLD
                     KNSFSGGQLFGKNPGGEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGL
                     GACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWR
                     QAQGHAFIDDGTWIDPLPFLNSSKK"
     gene            670..1314
                     /locus_tag="IV228_002951"
     CDS             670..1314
                     /locus_tag="IV228_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718778.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
BASE COUNT          514 a          217 c          322 g          330 t
ORIGIN      
        1 ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggagggta tgccgccgca
       61 ggaattactg aagacagtgc ctattataaa aattgtatgt ctattattaa tacaaataaa
      121 ttgatggagt acgatgagtt tgcgattaaa cactggagag aaggcggcgg aactggtggt
      181 acgattacag gaagctgggg caatccattc ccaggaagta gcttagataa aaattcgttc
      241 agtggaggac aactttttgg gaaaaatcca ggaggagaat tccgacctaa cgggttccat
      301 gatggactag actttggttc agtcgatcat ccaggaagtg agattcatgc ggttcatggc
      361 ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt tgggtgcgtg tgtgatcgtg
      421 attaattacg atggcttgaa tatggtgtat caagaatttg caaatagtac aggaaattct
      481 cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa ttgggatacg agatacagca
      541 cacttgcatt taggatttac tcgaatggat tggcgtcaag cacaaggaca tgcattcatt
      601 gatgatggta catggatcga tccgttacca tttttgaata gcagcaaaaa atagatcagg
      661 agaaaaaata tggaaaataa aaccttaaaa atcgtcttaa cagctgcaat tagcgttagt
      721 ttaatcggca atattgcgtt gttttggcag cttaaagaag aacaagagac aaatacctat
      781 aaaattgaag aagtggctaa aaacgcgtct gataagctca aaaaaaattc ttctgaggca
      841 caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga aatcgatcag
      901 ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca aagaaagacg
      961 gcagaagagt tcggtcaatt ggccattgat aaatcattaa gcagaaatga attaacggaa
     1021 aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctgc agacgatgat
     1081 caccaacatg acgactacgg aagttactct tttacattag gtgacgtgca aacttacgct
     1141 caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta tacaatcggc
     1201 aatccgcaat tcaaagatgt caaaccacaa aaaataaaag gtggattaac tgtcactgaa
     1261 aaacaagtag atggtcaatg gaaagtcata gactttagtt atttcactcg ttaggaggaa
     1321 gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa aaacgaaaaa gttatcttca
     1381 atg
//
LOCUS       DACDHL010000222         1383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDHL010000222 DACDHL010000000
VERSION     DACDHL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.229"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..654
                     /locus_tag="IV228_002952"
     CDS             <1..654
                     /locus_tag="IV228_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAP8718779.1"
                     /translation="GNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSIITSNKLTEY
                     DEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGGEFRPNGFHDG
                     LDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQEFANSTGNS
                     RVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLPFLNSSKK"
     gene            670..1314
                     /locus_tag="IV228_002953"
     CDS             670..1314
                     /locus_tag="IV228_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718780.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
BASE COUNT          504 a          223 c          319 g          337 t
ORIGIN      
        1 ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggcggtta tgccgctgca
       61 ggaattacgg aagatagtcc gtattataca ggttgtatgt caattatcac ttctaataaa
      121 ttaaccgaat atgatgaatt cgccatcaaa cattggggtg aaggtggcaa taacaacggc
      181 acgattactg gtgaatggac gaatccattc cctggcagca gtttagataa aagttccttc
      241 agcggtggtc aattatttgg aacgaatcca ggcggtgaat tccgtccgaa cggcttccat
      301 gatggattag attttggttc agttgatcat ccaggaagtg agattcatgc ggttcatggc
      361 ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt tgggcgcgtg tgtgatcgtg
      421 attaattacg atggcttgaa tatggtgtat caagaatttg caaatagtac aggaaattct
      481 cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa ttgggatacg agatacagca
      541 cacttgcatt taggatttac tcgaatggat tggcgtcaag cacaaggaca tgcattcatt
      601 gatgatggta catggatcga tccgttacca tttttgaata gcagtaaaaa atagatcagg
      661 agaaaaaata tggaaaataa aacgttaaaa atcgtcttaa cagctgcaat tagcgttagt
      721 ttaatcggca atattgcgtt gttttggcag ctgaaagagg aacaagcagc aaatacctat
      781 aaaattgaag aagtggctaa aaacgcgtct gagaagctca aaaaaaattc ctctgaggca
      841 caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga aatcgatcag
      901 ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca aagaaagacg
      961 gcagaagagt tcggtcaatt ggccattgat aaatcattaa gcagaaatga attaacggaa
     1021 aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctac agacgatgat
     1081 caccaacatg atgattacgg aagttactcc tttacattag gtgacgtgca aacttacgct
     1141 caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta tacaatcggc
     1201 aatccgcaat tcaaagatgt caaaccacaa aaaataaaag gtggattaac tgtcactgaa
     1261 aaacaagtag atggtcaatg gaaagtcata gactttagtt atttcactcg ttaggaggaa
     1321 gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa aaacgaaaaa gttatcttag
     1381 atg
//
LOCUS       DACDHL010000223         1369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDHL010000223 DACDHL010000000
VERSION     DACDHL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.230"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..1350
                     /locus_tag="IV228_002954"
     CDS             join(11..452,452..1350)
                     /locus_tag="IV228_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8718781.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          568 a          178 c          240 g          383 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag ttcaactttt ggggttcag
//
LOCUS       DACDHL010000224         1343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDHL010000224 DACDHL010000000
VERSION     DACDHL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.231"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(69..1208)
                     /locus_tag="IV228_002955"
     CDS             complement(69..1208)
                     /locus_tag="IV228_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8718782.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          351 a          289 c          249 g          454 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtttatta
       61 aaaaaatctt acgctaagta ggataaaata attccttgat atttttcgat catatccaaa
      121 taattatcga tttcaacata ctcatctact tgatgaggca aagtaacgac accaggccca
      181 aagacgacaa agtcgaagga atggttagct ttcgtaaatt cggctgcatc cgttgttgct
      241 gccgctccaa ctaaaggtaa tggctggctg aattgttgct gaatgcaatg aatcaaagga
      301 gaatctggat cagctttgac aggtattttg ttgtaatcga tcatcaattc caaatgatag
      361 tctgtctctt gattcaattc attgacgatt gattgcagca atgcaatgat tttatcattt
      421 gggtattctg gaatcgatcg aatgttccct tggagctgcg catggcttgg gatgctgttg
      481 acttgattcc cgccactgat caatgtaaca ttatggattg tttttcctag gacaggattc
      541 tcgattgttt cagcgaggtg attcatttca gcatttgctt tcgtaatgaa ttcatttagg
      601 tgattgattg cattataacc ttggtctggc atagagctat gcgcttcttt tccgtgcgag
      661 gtgactgtat agttgattga tcccatgtgc gtatacatca aactgtaatt ggttggctct
      721 ccgatgatca aggcatctaa gtcatctaca tagcctgctt tcgtgagctg ttccccacct
      781 agttcaccca cttcttctcc gacagtggct aataatttga ctgttccgtt aaatggtttt
      841 cctgactctt tcagttcgat catggcaatc accattgctg ccaagccact cttcatatct
      901 gtagcaccac gtccgtacaa acggttccca tggatttctg ctgcaaaggg tgcataggtc
      961 caagacgatt catcaccagc cgccacgaca tccatatgcc cagataggcc taacactttt
     1021 cctgattggc ccttttggta acgagcaatc aaattatctc taccatctct gtaagaaaca
     1081 atctctcctg ttatgtcatg ctttgccaaa agcttattca aataggctgc tacttcacct
     1141 tcgtttccat tcactgattt aattcggata atctcttgta aaatcgcgat tttttcttct
     1201 tttttcatct aatcctctcc cttgccattt ctttttattg tatttgagtg tgctcgaatt
     1261 ttcaaatagg agcaaccaaa gtaaaaatga agaaagagag ccactttatg aaaaatatgc
     1321 tgctacttcc ctcctatttt att
//
LOCUS       DACDHL010000225         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDHL010000225 DACDHL010000000
VERSION     DACDHL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.232"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1337
                     /locus_tag="IV228_002956"
     rRNA            <1..>1337
                     /locus_tag="IV228_002956"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACDHL010000226         1309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDHL010000226 DACDHL010000000
VERSION     DACDHL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.233"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..1158
                     /locus_tag="IV228_002957"
     CDS             46..1158
                     /locus_tag="IV228_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8718783.1"
                     /translation="MLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQ
                     QLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKA
                     LTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTD
                     EEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHT
                     KFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQV
                     LSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAI
                     IMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          467 a          242 c          218 g          382 t
ORIGIN      
        1 aaacatgcta aaaaaagaaa atattcattt ttaatgatcg ttgtcgtgtt gtttagcctt
       61 agtgcgttct ctacaggaac tattcttgga tggcaatttt acaataatga acactctcag
      121 atagttgccc atgctcaatc agctcaaaaa aagttgatac tgactaccaa tgaacaacag
      181 ctcgttgaaa aaatcgatca actattaaga aaaaatgatt atattggctc aatttacgta
      241 aagaaaaata atcgcatcat catggaaaaa ggctatggct atgcgaataa actcacgcaa
      301 caaccaaatg atccttctct ttattatcag attggttcgg ttcaaaaagc attgactact
      361 cttttgattt taaaacaagt cgagcgtggg cacctttctt tagatactaa gctggctcaa
      421 ttctatccac aaattgctgg cagccagcaa attactatcc gtgacttgct gtatatgcgc
      481 tcaggcttgc accgaactgc ttctccaacc ataccgatga ctgatgaaga agtcgttcaa
      541 tttgcgattc agcacttaaa atttacagat tatcagacct ctcattatga ttcattgaac
      601 tttaccatat tgactggaat tttgatcaaa ttaacaaatc aaccgtatga aaaattattg
      661 accgatgata tcatcgaacc tttaggatta cagcatacaa aattttatga ccaagtcaaa
      721 aattcatcca atcatgcact atcctatcaa atgtctaaag aggatgatta ctttaaacca
      781 ctgacagaat ctgaaacaga gatccataac gaattaggga ctggtaatat cagtatgtcc
      841 gtctatgatt taaatacgct gtttacacaa gttttatctg gaaaactagt ttccaaagac
      901 ttactcttct cattatggcg tgaaaatgcg aatggacatc catatagtgg tggggtatat
      961 agcggagata attatatcct gtcgcaagga aatatcgact cttttgatac atctgctata
     1021 tttagcaaag attcacagga tgctatcatc atggaaagca atataaaagc tgacaaaact
     1081 atcaaactgc ctactgctga tctaagaaac caaatctatg acctgatgga aggaactact
     1141 aagttggcaa atagctgata tgatgaaaag atagcaaata ctacttcttt gaatagcatt
     1201 tgcttaaaaa aagtactcga ctaaacgtag gaaagagtgt gggacaaatc taaaaatcaa
     1261 tttttgctac agactttaat tccaaataaa cagaacagaa agaggtgtt
//
LOCUS       DACDHL010000227         1307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDHL010000227 DACDHL010000000
VERSION     DACDHL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.234"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            267..428
                     /locus_tag="IV228_002958"
     CDS             267..428
                     /locus_tag="IV228_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718784.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            447..941
                     /locus_tag="IV228_002959"
     CDS             447..941
                     /locus_tag="IV228_002959"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP8718785.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          503 a          186 c          230 g          388 t
ORIGIN      
        1 gaaggcaaaa agtaattata gaaacgaaaa ataaatcata tcgggcactc agacatgaaa
       61 acaaccctag aaatatataa ctagtccaat gaaaaaaaag cgaatgccgt agaaaacaag
      121 gattctgcgt acattcatat ctcattttgc gagcatatct gctattactg tgactaaatg
      181 ttaatatggt taaaatgacc gttatatttt ttatataaat atcgtataat tagtatgaat
      241 atagaagaag gaaagaaggt gcaatcttgg aacctaaaga caagtattta gattacgcta
      301 gggtcaataa agataaattt attgaaacaa tcatcaaggg aaaaatccct gatgatgaaa
      361 aagatgcggt attcatggct ggaagcccag gagcaggtaa aacagaggtt gcactaggtt
      421 tagcttaaaa ttatgacaac catgttgtga ttgatgcgga ctattttaga gcacaatttc
      481 cagaatacaa tggaaagaac tcaagtgttt ttcagaaagc ttcttcttgg ttagttgaac
      541 aatcattaaa atatgtactg gaacatggct attctttcat actagaggat acttttgcta
      601 tccttagtgc agagaaaaat attattcgag cccttaaaaa taactttcgc gttacgattt
      661 tctatgttta ccaagatccc aaagttgcct gggatttcac tcgaaaacgt gaactagctg
      721 aaggacgtca tgtgccaaaa gaaatgttta tcaatgcgtt ttttaagtcc agagagaata
      781 ttgaaaaagt caaaaataga catcctgaag tagtccttca cataatgatt aaggattatc
      841 aaaacgacat ttctgaagtg cattatgcag cagataatat tcaattagta ctaccaatac
      901 aatatacttc aaaagagttg gaggaggaat tacatgactg agaaagaaca agtacaacaa
      961 atcgtgaaga agtataataa aagtatcgct gatttatcag aaaacgcctc agcgaaagaa
     1021 tttaaaacag taatgaatta cgtagcagat gaagcaaatc gtaagcagcg aaaactagtt
     1081 gggttggata aataaatttt ttaaaccata tccttgtaaa gttgagtttt ttattttaat
     1141 gaattaagac tattaaatag ataattattg taacttaaaa aacacagcta aaaattagtt
     1201 gtgtttttta ttaggtttac ttttctggta taaaaggaac tagctacttt ttcaaaattt
     1261 ctaaatcatt caatatgtag cgcttactaa attattcgcc ataaaat
//
LOCUS       DACDHL010000228         1278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDHL010000228 DACDHL010000000
VERSION     DACDHL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.235"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..1160
                     /locus_tag="IV228_002960"
     CDS             75..1160
                     /locus_tag="IV228_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8718786.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAKIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKYKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT          536 a          182 c          202 g          358 t
ORIGIN      
        1 ccttaataca tggtatagtt cacttgttga aaatcgtgaa tatatacaag tttctaagaa
       61 gaaggagaaa tttaatgtct acagaaaatc aatctcaaaa aatgattatt aaaaattggg
      121 gatataaagc aacgaatgtt tatattgaaa aatattataa atttcctagt tttttaattc
      181 agaattctaa atactctaaa atgaaagaca gctcaaaaat agcctatatg ctttttaaaa
      241 atgaatttcg tagagcctta catgaaaaat ggatcgacga tgatggtcta ttatatttgg
      301 aatttacaca agcagaccta atggacaaat taaattgcta ccaaggcaaa gtaaaaacca
      361 ttttaaacga gttagaaaca tataatttga tttcagtcgt aaaaggagaa tttaacccaa
      421 aaacgaaaaa aaatgaaaaa aataaatact atttacttca acctgaaata agtgccgatg
      481 atctttttat tcaaaattct ttcgatgacg atttaagcga aactattgat aaatcaacct
      541 cttctcggaa tgcggaaatc gcacgtcgag aggaaactgc taaaaccctt gataatactg
      601 gaaatgcgga aatcgcacgt cgagaaaaaa cagctgaaac ccttgataac actggaaatg
      661 cgaaaatcgc acaagattat tatattaata aatctttaga cactaagaga cacttaaaag
      721 acactgaaac agaccaatta caaaatcaag ttttgctaga taacttcgtt gaaatcatga
      781 aagaagacag cataaacaca tttgtaccag aaaaggtatt aagcttgata aaaacatttt
      841 ctaacactta cgaagaagcc caacaaaccg tcaccacaat acacaacgca aaatataaag
      901 ccgaggaact tgagaagacg gtagttgtgt atgaagaact tgaatcttac ggaattgatg
      961 cggataaagg attatatatg acgttgctga aagcttatca aaaacaaaag actgagaaag
     1021 taaataattt gcagaatttg atatttgtgt atgtaaaaaa ctggtttatt gaaaaagcta
     1081 ttgcggcaaa gcttgcaaac gaacaaagag aaacatttaa cgaattacca acagttagta
     1141 cggaaaattg gctagagtag ttgatcaatt ttaaattaga acttttttat aattttaatt
     1201 ttattacaaa tgttacaaat aaatagttat tattcaaagg ttttaagatt aaaagtttat
     1261 gaaaacaatc acaaacgt
//
LOCUS       DACDHL010000229         1269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDHL010000229 DACDHL010000000
VERSION     DACDHL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.236"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1269
                     /locus_tag="IV228_002961"
     CDS             <1..>1269
                     /locus_tag="IV228_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718787.1"
                     /translation="DDNGKEIPVQISDEGLAKYVYTSGYRRYSSKPGIILAGESSLAV
                     AYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIEDISQSFLLLAFTILEL
                     RIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSVTKIFGVDTSLKNGSNS
                     LLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGHRKDVTPDNGEEQSDDK
                     ATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAEDLTEKAKDTLEEKLDG
                     TRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQVNAAVKANTTTEKENQEGI
                     VIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPKTDPEQYRYKPNSDLENEK
                     RISDLSKVNQEDKAENEGQLPKTDPEQYRYR"
BASE COUNT          467 a          198 c          277 g          327 t
ORIGIN      
        1 gatgataatg ggaaagaaat tcctgttcaa attagtgacg aaggcttagc aaaatatgtc
       61 tatacttcag ggtatcgtcg atacagtagt aaaccaggta ttatcttagc tggtgaatct
      121 tctttggcgg tggcctattt atttatccta tttacgatcg ttacttgcat tattgagctg
      181 gtatttaaaa aattctattt agtgattgcc gcgtcaacgg actttgagac aggccaacgg
      241 atgaaaagtg cgatagaaga tatttctcaa tccttcttgt tattggcgtt tacgatttta
      301 gaattacgaa tctatacatt aatgctttca ggtatcggtg atttgcatgc ggcaggaaag
      361 atcaatggat tcttgtatgt tgttgcttta attgtgctaa ctattgcgct attcaaaggc
      421 tctcaatcgg ttacgaagat tttcggtgtt gatacgagtc tgaaaaacgg cagtaattct
      481 cttcttagca tgtttgcctt aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac
      541 cttgcaagtg ctggtaagag tggtttaagc catttaaatg atattcgtaa aaatggattt
      601 ggccatcgaa aagatgttac ccctgataac ggggaagaac aatcagacga taaagctaca
      661 actgtaccta atagtaagaa aacagtttcc gaaatgttcg ggaaagcgaa aaatggtgtt
      721 tctaaagccg cagaaggtct tggttacgtc aatgaacgtg ggttaaaagg aacagctgaa
      781 gatcttacag aaaaagccaa agacacctta gaagagaaat tagacggcac gagagctaaa
      841 gaattggctg aagctgtgaa gaatccatcc gaagcactag aaaaacaaaa agaaaaagct
      901 tcagaagtca aagaaggact aaaagatgcg tatggtgaag gacaagttaa tgcagcagta
      961 aaagctaata caacaaccga aaaagaaaat caagaaggta tagtgattcc ttcagtcaaa
     1021 gatcaccaag aagcgttgca ctctaatgaa aattcgattg aagagaaaaa ttccatagaa
     1081 gaaagaaatt cagaagaagg aaaaaatgga cagttaccaa aaactgatcc agaacaatat
     1141 cgctataaac ctaacagtga ccttgaaaat gagaagcgaa tttcagattt atcaaaagta
     1201 aatcaagaag acaaagcaga aaatgaggga cagttaccaa aaactgatcc agaacaatat
     1261 cggtataga
//
LOCUS       DACDHL010000230         1264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDHL010000230 DACDHL010000000
VERSION     DACDHL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.237"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..75
                     /locus_tag="IV228_002962"
     CDS             <1..75
                     /locus_tag="IV228_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8718788.1"
                     /translation="EFLFPKFLEDSSELQDIYNYLYRR"
     gene            80..1027
                     /locus_tag="IV228_002963"
     CDS             80..1027
                     /locus_tag="IV228_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8718789.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          407 a          186 c          268 g          403 t
ORIGIN      
        1 gaatttttgt ttcctaaatt tttagaagat tcttcggagc ttcaagatat ctataattat
       61 ttatatcgga ggtaaaagga tgagctgtta tcttatcacc ggtggggctg gcttcattgg
      121 ttctacctta gccaatcatt taggagaaga acatgtggta atcgtcgtcg atgacttatc
      181 aatgggaaaa gtagaaaatc ttgatatgac gaaaaatatc acttttattg agggagatgt
      241 cgctgagaaa tccttgatgg aagagatcat gaaagctaat aaatttgatt atatctttca
      301 tttagcagca gttgctagtg tggcagattc agttgcccga ccagtagaaa cacatcgtgt
      361 gaattttgaa agtgtattaa tgattttaga attgatccgc aagtatcaac ctgatttaaa
      421 acggatcgtg tttagttctt cagcggctgt ttatggggat gaaccgactt taccaaaaaa
      481 agaagagtcc gtgattcgcc cattgacgcc ttatgcggtt gataaattcg ctgcagaaca
      541 atatgtgcta gattattgtc atttatatga tgtacccggt agcgcagttc gttttttcaa
      601 tgtctatggt ccaaatcaaa atccgaattc tccatacagt ggtgtgattt ctattctcgt
      661 agatcgctat aagaaacaaa tcgtaggaga agcaacttca tttacattat ttggtgatgg
      721 aagccagtct agagactttg tttatataga tgatgtaatc caagcattac ttttagttgc
      781 gaatgaggaa gctgcactgg ggaaacaatt caatgttgga acgggtaaag caaccacact
      841 tttggaattg attcatgcaa tcaatgaaat tctcggtgta gaattgacac ttgaatacaa
      901 agaagagcgt tcgggtgata tccatgactc tctagcagat attactaaga tccagtcaat
      961 tggctatcaa ccgaaatatg gtattttaag tggaatgact aattatttga aaactgaaat
     1021 caattaaaac atactaagat aagattagag ggtaaattca ggctctttgt caataaggac
     1081 tgatgagttt tgtaaaatca aatctgggat agaagggctc tttgtcaaat gatgttggta
     1141 aagagaaatc ttagaataat ttggacagaa cagaaagaag aaaacctctt tctgttctgt
     1201 ttatttggtt aaaaatgttt tgccatgacg aatcataatt cgtagtttga aattgtaaaa
     1261 gttg
//
LOCUS       DACDHL010000231         1264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDHL010000231 DACDHL010000000
VERSION     DACDHL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.238"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1102)
                     /locus_tag="IV228_002964"
     CDS             complement(<1..1102)
                     /locus_tag="IV228_002964"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024831.1,HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8718790.1"
                     /translation="MKLLYILNVANKVNNFSKASMMAAKQTGLEFYIAGNWKYKSDEE
                     RKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVVGRI
                     AGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQK
                     LRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELNENK
                     NHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLL
                     AAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEI
                     AGAINRLADNKLLCESMGAHNLDKIKDFDLSVV"
BASE COUNT          401 a          235 c          145 g          483 t
ORIGIN      
        1 tcacaactga taaatcaaaa tcttttatct tgtctaaatt atgtgccccc atagattcac
       61 acaataattt gttatcagcc aatctattta tcgcaccagc aatttcttct tcgtctttag
      121 gattgattag aaatccaccc ttaccatctt ctataagatc tacatttcct cttatattac
      181 cgacaataca tggtaaacca ctagccatcg cttccattaa agaggcgttg aggccttccc
      241 ttaatgaggg tagaatatat atgtttgcag cagctaataa tttgggtata tcacttctat
      301 atcctagtaa aaaaactcgc tgatgtaatc ctaattcatt agcaatattt tgtagtgtct
      361 ccctcatttc tcctacccca gcgataatat aatagatatc tgtatgcagc ggaatttttt
      421 gtaatgcatg tattactgat aaatggtttt tattctcatt tagctctcct acagataaaa
      481 tgatttttgc tgtatcaggt atattaagtt ctttacatat actttctcta aaattcctta
      541 ttgatgtgga agacataaac ctatttgtat ctatacccac acctggaata taataagctt
      601 ttccaccttt cctaagacga agtttttgag ctaaagtata atcctcatga tttattgtaa
      661 tcaaaacatc tgtataatga gctagccaac gttccatagg ataatatata aaccaattta
      721 ttttaggagc acctgaataa aaatgaaatc catgtgcctg gtaaattact tttttgactt
      781 tacatttaag accagcaatc cgtccgacaa cacctccaat aggtgtatta caatggataa
      841 tatctatttt ttcatttttt ataatctcta caatctgatg ataagctttt atatttttta
      901 aatttagtgg gtttctataa aaatcaactt ggtatatttt tattccatat ttcacttcat
      961 cagcttttct ttcctcatcg gatttgtatt tccagttacc agctatataa aattctaaac
     1021 ccgtttgttt agctgccatc atactagctt tagaaaagtt atttacttta ttagctacat
     1081 ttaaaatata caataatttc ataattaatt cttctccata taaaaccgat attcattcat
     1141 aattcttcct tcattagtag aatatttacg ttctaaaatg aactcattct ttttataaaa
     1201 attattcaca acatcattat ttaatgcatc tgtttctagt gcaatatatt catattttga
     1261 aaaa
//
LOCUS       DACDHL010000232         1247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDHL010000232 DACDHL010000000
VERSION     DACDHL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.239"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            507..743
                     /locus_tag="IV228_002965"
     CDS             507..743
                     /locus_tag="IV228_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8718791.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            740..1153
                     /locus_tag="IV228_002966"
     CDS             740..1153
                     /locus_tag="IV228_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8718792.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
BASE COUNT          493 a          156 c          222 g          376 t
ORIGIN      
        1 atcatatcca taaatctctt acatatataa gcgagaagga aaatcaagat tataatactg
       61 tagtgagaga agcaattagt gctgtcgaaa gttgtcttat cgaattttca ggattaccag
      121 ctaataaaaa aatactttgg gaactgctat aaaagcaatt aaagacaaat cgaataacaa
      181 tgtcgatctt acatttttaa aaccatttga gacaatgtat ggtttggcga gtaataatgg
      241 aatacgacat gcagaaaatg aaaaaacaat tgtctcagat ttacctgatg cagttttggt
      301 gttaacaaca tgttcagccg taattaatta tttgggagta aaaatagcat atgactccaa
      361 taaatcatga aaagttaaaa ttcatccaag agctacatta taactattaa tttccaactc
      421 tttaaaagtt tagtaattga aggaagtaat aagggtgatt gaacaatacg gatatacggt
      481 gtatacttat cgtaggagtt gatgatatga atattaaaaa acgcaaaatc agagaagtag
      541 gtaattcaat agttgtaact ttgtcaaaag agagtttatt gcaaaaaggt ttgaaacctg
      601 gagatactat ttttattgat gaagataaaa tgatggaagc tattgtaaaa gaggaaagca
      661 agatagatct tgaaattgat atgtatgtta accaagcttt atctgagtat gatgcagctt
      721 tcaaggaatt agttgaaaaa tgatctatct aagtgaaaaa gatattgtaa aattgaatgt
      781 tattcaaata aagaaatatt caccgcgaga aacaatcgga gtcattgaca aaaatgcgct
      841 gagtatggct gtaaatcaac ctaaacagca tatttttgga aaagatctgt atcctgataa
      901 atttagcaaa gcagctgttc tgctaataaa tctgatcaaa aagcatccat ttcataatgc
      961 taacaaacgt acagcatttt tagccgcgta tgtttttctc aagctaaatg gctattcact
     1021 aaaaatgaaa aatcaggaag tagtagaatt tgtagttgaa attgctacgt atcagggtga
     1081 ttttgatgac ttaaaagagg caacaacaga tatactaaaa aagaattcaa caaagaccga
     1141 ttcaaaaaag tagaagcaaa ctagggattc agttaaatct gaagatttag tttgttcttt
     1201 attttagttt acataagata cattatgcga agttcattat tttaaaa
//
LOCUS       DACDHL010000233         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDHL010000233 DACDHL010000000
VERSION     DACDHL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.240"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(7..1065)
                     /locus_tag="IV228_002967"
     CDS             complement(7..1065)
                     /locus_tag="IV228_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8718793.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          492 a          205 c          148 g          400 t
ORIGIN      
        1 ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat aataccagaa
       61 aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca tttcatctat
      121 aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca ctttacttct
      181 aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga tacatataaa
      241 agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt ttccatgtgc
      301 tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa attttgcatc
      361 gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc ttataacttt
      421 aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat cactagaatg
      481 ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg actctataac
      541 ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag cataattagt
      601 accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt tgatttttac
      661 aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca aaagaaataa
      721 tactaaaaaa gaacaaatta ctacctgggc acctctagac gaagaaagtg taatataaaa
      781 tatttgtacg ataaaattca tcatataaaa aacacgaatc gtttttgtta tttttgaaat
      841 taatacacta attaaactta aaataataga agtatatcca aacatagctc caacatttgg
      901 acttgtatag acaccaaata accgatgttc aataaaacct tgtcttgcaa gtaatcctgg
      961 cgttcctgtt gggacatgat aagaaatcaa aaaaataaat tgatagatag atattagcgc
     1021 agctaaaaaa attatgataa tgaaaacatt attaaaatac accatttttt tctttttttc
     1081 ctcttcactt tgtgaaaagt ctgcaggaaa aattactatc aaagtaatca gcaaatatcc
     1141 aaaattataa atagatggaa ttaaaaaatt ttctctgtta atgattatag ttaagataaa
     1201 actaatcatt gctccaatca gtagccaaga aaaacgcatt ttaaa
//
LOCUS       DACDHL010000234         1204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDHL010000234 DACDHL010000000
VERSION     DACDHL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.241"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            298..675
                     /locus_tag="IV228_002968"
                     /pseudo
     CDS             298..675
                     /locus_tag="IV228_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            675..>1204
                     /locus_tag="IV228_002969"
     CDS             675..>1204
                     /locus_tag="IV228_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HAP8718794.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVAT"
BASE COUNT          396 a          196 c          263 g          349 t
ORIGIN      
        1 atagctatga aaacctagtg attgttactg aggaggttca tcgggtaata cacgctacac
       61 aaagtgaaac gattgaggaa cttctaaaat atctaaaatt aaatccaaaa caaaaagaaa
      121 aactcaatga gttgagatta aaagttggta acgaagaaat atcttaataa atatttgtag
      181 gacgggaaaa cagaatgtct tttatagacg agggaacgcc gtatgcggtg aaagtcgcac
      241 gtacggtgtg gactggggga aaagacggag ataatatcaa actcttacct atcagtatcg
      301 attaatccag attttattga aacggtaaat tatgtcacaa actttcaacg agaaatgcgt
      361 aaagtagaag caatgactgt tttagctaca caatccccac aagaaatggt gccagaaaat
      421 atgtcttctg ataacttctc gaaactgaag aaagtctttg aattttcttc tacgaagatc
      481 tttatgcgta tggatgaatc tattttacgt catatagaag gattagtggg taaatcaatg
      541 acgaactcag aattagaatc cattcctcaa caaaatcaag gagacgctgt gatcaatttt
      601 ggctcaaaag aaaccattcg tgttcatttt agtccgaaca aacgtcaatt aaaattgttt
      661 gatggaggaa aataatggaa aaccaaaatg aaagtttaat caagcagtat gttaagcgtc
      721 gtgcaaagcg gcgcttattt ttgtggttgt ttggtacttc tgcaggtttg attactattt
      781 tgatcactgt gttcgtgacc ctatttctaa tacttgcggc gggctcgatt gataattcgg
      841 attcagattc aagcagtggt ggcgaagctt ttacaggaga atattcggaa ggcttaccga
      901 tttataaaga aatcaaagga agaggccctt tttctgatga gattgcgcaa tatgccgtag
      961 gtgcagcagt gaaatataaa ttattaccaa gtgtgatcct ttcgcaatac ggttacgaat
     1021 cagcgtttgg tacatcggcg tccgcaagaa atgatctcaa ctattttggg attacttggt
     1081 ttgatggttg tctgtttcct aaaggaactg cacgaggaat tggtggcatt gaaggtggct
     1141 ggtacatgaa gtttcccaac tcaaaagctg cattttccta ttacggcttt atggtagcca
     1201 caca
//
LOCUS       DACDHL010000235         1199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDHL010000235 DACDHL010000000
VERSION     DACDHL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.242"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1041)
                     /locus_tag="IV228_002970"
     CDS             complement(<1..1041)
                     /locus_tag="IV228_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8718795.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQ"
BASE COUNT          479 a          177 c          193 g          350 t
ORIGIN      
        1 ctggttacct agtaaatatt gatcattatt aatcatatca ggatgataga ttgactcttt
       61 gtttgcataa tcagtatgat tgtaattttc agctatagca acagcgtctt caggtgctat
      121 aagtgttaat gaatctttgt tagcatggat agaattgctt actcctgaga gatgcaatat
      181 tagtgcaaga aacgtaaaga ccattatttt ccatgaatta tttttagtag aaaaaatata
      241 atagatcact atactcatcg tataggctat aaataaagaa gaaaaaccat ttaaccgcca
      301 gacaaattgt agactcttaa ctggtgtatg gttaaaaagt tgccaaggaa ataaattggt
      361 tgaacaaaaa agaacaaata caccaagaaa gaaaataaat aaatcatcag cagttaattt
      421 ttttataaaa ataaaagcac caatcaatcc gagaaataag aataatccaa tagtatagtt
      481 gttatagtta ttatttaaaa tgtgtgtcag catatcacta ggagccatac cacttaattc
      541 ttttcccagt gggactttca attcttgtgc tctagattgt tgaataaaag gaattaagaa
      601 tcccgcactt aaaaaaaagg tgaggacggt agcttttaaa agacttaaga gtctagcgat
      661 tttttggtcc caaaaataaa aagaaagaaa caacgttcct ccaataaata cagaaaccat
      721 agcgacagaa agtaaatgag tatacactac tagcgtcatg ccaatagaca accagtacca
      781 tttttggtaa tctctaatat aaatttcgta acaccccatt agtatcaaag gcaaaaatgt
      841 taaagcgaca gcttctccca atgaagcacg atggaatatg tcaatggcac gataggcgga
      901 aaatgtataa ataatcgaaa ataacagcga aatataacga ttgtttttta tttgtttcat
      961 agaaaaataa gaaacgacca ttgttaaaaa agtgataaaa aaatagtaga tattataact
     1021 taaaacaaga tttcccatca ttttataaaa taaaaaagct ggataaagag taagccatgg
     1081 ataaaaaata ttcatcattg ttccatgatg atcgaagtta ttgaagttta ctggagaact
     1141 ccatacatta tctagtccta tgattcgaga taaatgaaag taagtatcct gattgacac
//
LOCUS       DACDHL010000236         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDHL010000236 DACDHL010000000
VERSION     DACDHL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.243"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV228_002971"
     CDS             <1..1076
                     /locus_tag="IV228_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8718796.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACDHL010000237         1167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDHL010000237 DACDHL010000000
VERSION     DACDHL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.244"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            56..727
                     /locus_tag="IV228_002972"
     CDS             56..727
                     /locus_tag="IV228_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8718797.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          426 a          178 c          168 g          395 t
ORIGIN      
        1 atggcatgaa cagaattaaa atatcagtta aaataaaagt taaggagcat tgtgtatgac
       61 aattaaaact agtctccaaa atcaagctta ccaatctatt cgccgacaaa ttatctatgc
      121 tgacttagaa cctggaaaaa aagtatcaga aaaaaaatta gaagaaacac tttctattgg
      181 tcgtacacca atacgtgaag cactgattca actccgacaa caaggtcttg ttgatacaat
      241 tcctcaaagc ggaaattata tctcattaat tgatttagat tcggccaaaa atgctcgatt
      301 tgtcagagaa catttggaac gacaaattat gttagagtgt tgtgccaaaa tgaataacca
      361 aaaaaaacgg attttagaaa ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa
      421 tgctcgtagt ttttttcata atgataattt attccataaa gcttgttttg aaattgcaga
      481 gcgagaagaa gtatggcaat ggttagaaaa taacaataca cacttagaac gatttcgatg
      541 gctacgagta aaaacggaag gattaaagtg ggaaaaaatt atggaacaac attatcaact
      601 atttgaggca ttaacaaaac aagatccaga agaagctaac tttctaacat cgttgcatct
      661 tcatttaatg cttaatgagc aaaatattgt tttagctact catccaaaat atttcaaaaa
      721 tatataactt ttaactcttt ttaaattaaa tggttgttct gatcagaaaa atatcgcttg
      781 ttttcttatt atgtaatata tttcataaac tattctgcat aacaactatt gtttgtactt
      841 tttacattgg ataactaatt atgactgctg ataacttttg atattaaact agtatctcca
      901 atacaaataa aaaatagata tcttgttcat caaaattagt tcgtggatac ttaccttcat
      961 ttacatcatt cagcggtttc ccattttctt ttattctatt atttcatttt ctaatagact
     1021 tggcttcatt tcgcaatact ttttttcaat aggcatcaaa ttggtgttgt ttagatattt
     1081 agttgtagtt ttgtgacatg ataagtatgc tcctttttct cttttccact aatacaacga
     1141 tcaaagggtg cttttttcaa aaaacta
//
LOCUS       DACDHL010000238         1163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDHL010000238 DACDHL010000000
VERSION     DACDHL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.245"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..423)
                     /locus_tag="IV228_002973"
     CDS             complement(<1..423)
                     /locus_tag="IV228_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8718798.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSAD"
     gene            774..1073
                     /locus_tag="IV228_002974"
     CDS             774..1073
                     /locus_tag="IV228_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8718799.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          419 a          165 c          193 g          386 t
ORIGIN      
        1 atctgcgctt gataagacag agtgctcaaa ggtactttct tttatgtttg aaccagataa
       61 atttacatag tccattttac aattctcgaa taacgtgttc ccaatataac tttccatcaa
      121 tttagtacct gttaatttac aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa
      181 tatacagcct gaaaaatcac agttttcgaa tatacagtcg gtaatctcca ttctttgaaa
      241 atcattggaa gcaaaaaatg catacttgaa aatagatttg taaataattg gatggcagat
      301 tatttggttt tcgatgacct ctttttgtga aaggatatta tacagttcaa aatcttcgtt
      361 ttcatagata tgaaaaaagg atcctttatt caagcttgct tcattaactt taggaaaaat
      421 catatttaat tacctccatt ttcataaagt catattaaca aaagctaaac caacttacat
      481 tagcagaaaa tgagataggt tttcgccttt ttcgataacg atagtgtcgc tatctcttga
      541 aacgttctta gaattgccct gagagacaca aattaggcgt aattctaata aaaatcttag
      601 aataaaaatt tttactgatt taatttagaa tgtttttaaa ataaaaatcg ttgccataac
      661 agtgtattga aaggttttaa caatccagta atgatttaaa aaaatctaga tgtagcgtat
      721 actttgttta tagaatgtag tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta
      781 aaagtgaaga aatagaaaac atggtactaa aattatatga tctcgtttta aatccagaga
      841 tcaaagaaaa ggaacgtata cttttgatag acgcaaagac aggcttagaa aaaggtcagt
      901 attatcctaa agtcataaat aatttggaac gtagtttacg tccgttagca atcaggggag
      961 aattgtctaa accagtatca ccgttttata tggagatatc aacaatagga aaatttgaaa
     1021 aagaattagg tcgaggaatg gcttcggcac cgattacttt tgggcattta taaaaacgag
     1081 ttattcatct caatgaatta aaaagtatag tcaatctcat aatgcaggct aggttcattt
     1141 atttgaacag agtctgtatt ttt
//
LOCUS       DACDHL010000239         1155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDHL010000239 DACDHL010000000
VERSION     DACDHL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.246"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..154
                     /locus_tag="IV228_002975"
     CDS             <1..154
                     /locus_tag="IV228_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8718800.1"
                     /translation="NKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIQA"
     gene            206..352
                     /locus_tag="IV228_002976"
                     /pseudo
     CDS             206..352
                     /locus_tag="IV228_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            429..758
                     /locus_tag="IV228_002977"
     CDS             429..758
                     /locus_tag="IV228_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8718801.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            748..1035
                     /locus_tag="IV228_002978"
     CDS             748..1035
                     /locus_tag="IV228_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8718802.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          420 a          155 c          221 g          359 t
ORIGIN      
        1 caataaattc tatcgaagtt tacgcactgc ctctaccacg attaaaggca tggaagccat
       61 tcgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
      121 tgaaatcaag gtattattgg gaatccaagc ttaaatcata gataccgtaa gggattttat
      181 tctttattta aaactttgca acagaaccca atcatttgag aagttaaaac ctacacatga
      241 aggtaggtat tttagaagat tgaatagaca acaccgggtt gtttataagg tagatgaaga
      301 tgaacacgtg gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt
      361 aaatacatta tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag
      421 ggatttaaat gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta
      481 aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc
      541 tttatgaatt tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg
      601 atataattgc agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa
      661 acaaaatgca ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa
      721 tgactaaaga ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc
      781 attaagtatt taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt
      841 tatgatgaaa ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt
      901 atttggtcta gtgggttcaa atatgcgggc acaacatata gagtagctta cgagattata
      961 gaaaacaagg ttattccagt attgttatgt ggaacgcatg aaaactttta tgaacagttg
     1021 aaaaaattaa tctagtgatt tgttgtcgtt cttaagaagg acggcttctt ttttatcgaa
     1081 aatctgattc gtctacatga ctaaagtcgc gagtattctc tgcttctcga taaattaaat
     1141 aacgtcatta ctttg
//
LOCUS       DACDHL010000240         1136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDHL010000240 DACDHL010000000
VERSION     DACDHL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.247"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1061)
                     /locus_tag="IV228_002979"
     CDS             complement(<1..1061)
                     /locus_tag="IV228_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8718803.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          301 a          254 c          182 g          399 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaac
//
LOCUS       DACDHL010000241         1126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDHL010000241 DACDHL010000000
VERSION     DACDHL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.248"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            83..>1126
                     /locus_tag="IV228_002980"
     CDS             83..>1126
                     /locus_tag="IV228_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8718804.1"
                     /translation="MPYLLLQKIIETKKDINEGIENISDSKISPVSSIITEQTQIDIS
                     ELEDKHAIQQNDHFSEQNEFIERFNQLTIRSKLHYSKRDLWNFHTAMLGDSLVVLAGL
                     SGTGKSQLVTSYAKALQLSDTQIKFISVRPFWEDDSDLLGYADTVNSVYRPGDSGLID
                     ALLEASRHKENIYMVCFDEMNLARVEHYFSQFLSVLEMEPNTRKIKLYNEDLENKLYN
                     SSTYPSTIEVGQNILFVGTINTDESTHQFSDKVLDRSNIISLELVPFHEISEVNDYIQ
                     SDQKKRNQLKYDDFKNFKNSNPTNGLTQKEKEMFWKLHLAINSKDKNIGIGWRILKQI
                     DEYLKNLPVQNDLS"
BASE COUNT          440 a          161 c          171 g          354 t
ORIGIN      
        1 ttacaaaaag tttattgatg aaggtagtaa aaactatgta ggttacgatg attttataaa
       61 tcaaacaaaa gatataattt ttgtgcccta tctattactt caaaaaatta tagaaactaa
      121 aaaagatatt aacgaaggca tagaaaatat ctctgattcc aaaatatctc cagtaagttc
      181 aattattact gaacaaacgc aaatagatat atctgagtta gaagataaac acgctattca
      241 acaaaatgat cacttctctg aacagaatga atttatagaa agatttaatc agctcacaat
      301 tcgtagtaaa ttacattact caaaacgaga cttatggaat tttcatactg ctatgttggg
      361 tgatagtttg gtggtattag ctggactaag tggtaccggt aaatcacaat tagtaacatc
      421 ctatgcaaaa gcacttcaat tatcagatac acaaataaag tttatttctg tgagaccctt
      481 ttgggaggat gattctgatt tattgggcta tgctgatact gtaaattctg tctatcgtcc
      541 aggagatagc ggtctaattg atgcactatt agaagcatca agacataaag aaaatattta
      601 tatggtttgt tttgatgaga tgaatttagc tcgggttgaa cattatttct cccaatttct
      661 ttcagtttta gaaatggagc caaatactag gaaaatcaaa ctgtataacg aagatctaga
      721 aaataaatta tataactctt caacctatcc ttctacaatt gaagtaggac aaaacatatt
      781 atttgtagga acaataaata cagatgaatc tactcatcaa ttctcagata aagttttgga
      841 tagatcaaac attatttcac ttgagttagt accattccat gaaattagtg aagtaaatga
      901 ctatattcaa tcagatcaaa agaaacgtaa tcaacttaaa tatgatgatt tcaaaaattt
      961 taaaaactct aaccctacta atggtttgac ccagaaagaa aaagaaatgt tttggaaact
     1021 ccatttagca attaattcta aagacaaaaa tatcggtatc ggttggagaa tattaaaaca
     1081 aattgatgaa tatttaaaaa atcttccagt gcaaaatgac ttatca
//
LOCUS       DACDHL010000242         1104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDHL010000242 DACDHL010000000
VERSION     DACDHL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.249"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..914
                     /locus_tag="IV228_002981"
     CDS             <1..914
                     /locus_tag="IV228_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8718805.1"
                     /translation="VEVGGTVDLTDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEG
                     VIEVTYPDGTKDTVKVPVEVTDNRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLP
                     KGTTYTWKEKVDVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEG
                     DQIVTGKTEPNAEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNT
                     SEPTNVVVSSNEKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSN
                     IFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          435 a          159 c          233 g          277 t
ORIGIN      
        1 aagtagaagt tggaggcaca gtagatctaa cagataatgt tactaacttg ccaacattac
       61 cagaaggaac aacagtaaca gatgtcactc ctgatggtac gattgatacc aacacaccag
      121 gtaattacga aggggttatc gaagtaacgt atccagatgg tacgaaagat acagtgaaag
      181 ttccagtaga agtgacagac aatcgttctg atgccgataa atacaatcct gaaggacaaa
      241 aagtgactac agacttaaat aaagagccag acgcatctga gggaattaag aacaaagaag
      301 atctaccaaa aggaactact tatacttgga aagagaaagt agatgttagt acagctggaa
      361 acaaaaaagg gattgtagta gtaacatatc cagatggatc taaagaagaa gtagaggtta
      421 ctatttctgt agaagataaa aaagcaccga ataaacctca agttgatcct attacagagg
      481 gtgaccaaat tgttactggt aaaactgaac caaatgcaga ggtgacagtt acattaccaa
      541 acgggagtca ataccatggg acagctgata aaaatggtaa ttttacagtt aaagttccta
      601 aattagaggc aggaacaaaa gtgatagtaa ctgcaactga tgaatctggt aatactagtg
      661 aacctactaa tgtagttgtt tcttcaaacg aaaaagacag tgaaaaagcc gttagtaaag
      721 ataataagac tgacaatcaa ggtagcaagc aaaacaaaaa tagaggcaaa agcagtcctc
      781 aaaagcagtc ctcaaaagct tatcctaaga cgggagagat tgatagtaat atctttacta
      841 ttagtggtgg tttaatcctg ttaggaactt tagggttatt agggtataaa aatcgaaaaa
      901 aagagaatga atagaaaatc aatttcttaa atcttctctg aaaagaggaa gaatcttttt
      961 cttcctcttt ttgtttttta ttttgggaga taggttgtta gttaaaaatg aagatacttt
     1021 tcatagaagg gaaaagaatg tgattaagtg aaaaaaatag taattattgg atcaaaccat
     1081 tcggggctag ctgcagctcg attt
//
LOCUS       DACDHL010000243         1092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDHL010000243 DACDHL010000000
VERSION     DACDHL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.250"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            89..1024
                     /locus_tag="IV228_002982"
     CDS             89..1024
                     /locus_tag="IV228_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8718806.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT          378 a          174 c          247 g          293 t
ORIGIN      
        1 gttataaaaa tgaacattgc atagtagaga ttctttgatt atactaattc aaggttatat
       61 gaaaaaagag aaatgagatg gatgatttat gaaactttgg atgaaaatcc ttttaggcgt
      121 cgctttagct ctgattgttg cgttagtgtt tgcagggaat tatttttata attatgctgt
      181 tgtgccttct gagaaagact ttttagacga agatacacct ggtacaacac agacatccaa
      241 agaaacggat gcacataaat ggtttagttc aaaagaaaac cgaagcgatt ggaccatcac
      301 ttcaaaagac ggtctgaaat tatcagcgat ttatcttcct gctgaaacga aaagtgaaaa
      361 aacagctctt gtggctcatg gctatatggg aaacgctgaa acgatgacca attacgcaaa
      421 gatgtttcat gatatgggtt acaatgtcct tgttcctgat gcaagaggac atggaaaaag
      481 cgaaggggat tacattggat ttggatggcc agagcgtaaa gactatgtac agtggataaa
      541 caaagtgcta gaaaacaatg gaaaatcgca agaaatcgtt ctatacggtg tcagcatggg
      601 agcagcaaca gttatgatga ctagcggaga aaaactacca gataatgtga aagcaatcat
      661 tgaagattgc ggttattctt ccgttcatga tgagcttgct tatcagctag acgatatgtt
      721 tagtcttcca gcatttccgc ttatgcaagt aacgagtcta gtgacaaaag tccgtgctgg
      781 ctactttttc ggtgaagcaa atgcagtgga acaattgaag aaaaaccagc gcccaatgct
      841 ctttatacat ggagacgaag atacatttgt accatttgaa atgttagata aggtctatcg
      901 tgcaacgaag ggaccaaaag aaaagtatgt tgtaaaagga gccgagcatg cggaagcata
      961 caagacagat cctgaaaaat atcagcaagt agtccaacaa tttttaagcc aatatgttca
     1021 ttgaacgagt ttaaaagtag gggatgaaaa gaaagaagcc tttgactgga tccagtcaaa
     1081 ggcttctttt at
//
LOCUS       DACDHL010000244         1065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDHL010000244 DACDHL010000000
VERSION     DACDHL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.251"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            78..>1065
                     /locus_tag="IV228_002983"
     CDS             78..>1065
                     /locus_tag="IV228_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8718807.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPP"
BASE COUNT          360 a          204 c          207 g          294 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat caccacaaaa taaacaactg
       61 agaggacgat acgctatatg aatgattcta tcaaaaaaat gctgagaata acagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cccttcagaa gaaaaagacg ttggtcgtcg
      181 atgctgttct ctcgcctact cctcgtgctt gtagaagttg tggttctact gtggtagatg
      241 gaaacgggaa agcaattata gtgaaaaatg gaaaaaagga aacgattgtc cgttttgaac
      301 aatacaatca tatgcctttg gttatgcgcc taaaaaagca acgctatacc tgtaaaaact
      361 gtcgcaccca ttggacggct caaagttatt ttgtccaacc cagacattca atcgcaaatc
      421 atgttagata taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga
      481 aaagctgtca ggtatctttg accaccgtta ttcgtacatt gaaagagttt aaaagctatt
      541 taccaaagca atctaagaag atcctcccca gagtattgat ggttgacgaa tttcgttcgc
      601 atgcttccat agaagataag atgagcttta tttgcgcgga tggcgaaaca ggaaaattaa
      661 tagatgtttt gcctacgcgt aaattacctc gattaacaag ctatttctta ggttgtacca
      721 atccagaaga agtagaattc ttggtgacag acatgaacgc cgcctacttc cagctcacca
      781 aacgtgttct gccaaatgcg aaagtcgtga ttgatcggtt tcatattgtc aaacacatga
      841 atcaagcgtt caatgagttg cgtatccgtg aaatgaatga acttcgtaaa gccggacaga
      901 aaagccaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca
      961 tcaaccatta tgaatacaaa acatggaaaa gtttccgagc accaaaatac ccatttctta
     1021 ctgaagcaat gatgattgat cgattgcttg agttttctcc gcccc
//
LOCUS       DACDHL010000245         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDHL010000245 DACDHL010000000
VERSION     DACDHL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.252"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            171..>1058
                     /locus_tag="IV228_002984"
     CDS             171..>1058
                     /locus_tag="IV228_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP8718808.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTL"
BASE COUNT          372 a          186 c          187 g          313 t
ORIGIN      
        1 agtctaactt ttggggtgca catcactttg aggggcggta cataaaaatt tattatttaa
       61 cacgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg gtaaatcaac
      121 aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg aggactacat atgcgttcat
      181 caaaatatac tgaacattat acagatactt ttataacatt caaggaaatt atgagaactg
      241 tttctaacat ataccacaat tgtgtaccag ataagattaa aaaccgaaga aatactgatc
      301 aactgaagca acgcgataca gtgattattg cttgtgttat atggggcata attaatggct
      361 atactagcca aagagccacg tatagagcgg tttgttccgt cttatttcct aatggtgact
      421 ttcctagtag gagtcgattc actcgcttaa gttcaaactt agcttacact atcaagatta
      481 ttcgatactt tttcatcaag aagctcacca aaggtgaact agtcggaatt atagatagtt
      541 ttcctagtcc attatgtaaa ccagttagaa atagacaagc aaaactattg aatcaaatag
      601 ccaaagttgg ctataactca acaaaaaaat cttattttta tggtcttaaa attcatatga
      661 tcgttactaa gactggcttt ccgattactt actcaatcac gaatccaggt gtccatgacg
      721 tgaaagtgtt agagacttta tcggaagaag cgaatcttcc caatatccta ggggacaaag
      781 gatatattag ccataaaatc catgaaaagc ttgcccttaa gggcattact atatccgttc
      841 cgccacgtaa aaacatggat aaatcagaaa aactagacca cagcttactt ggaaaacaaa
      901 gaaaaacagt tgagactgtt ttttcttcct tagaaaaatt aggttgtcaa aatttcaact
      961 cacgttctgt taaagggcta gagagtagat ttgaaagcat attactggct tacagtgttc
     1021 tattaagtcg agcacaacga cgttttgaag gaactttg
//
LOCUS       DACDHL010000246         1036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDHL010000246 DACDHL010000000
VERSION     DACDHL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.253"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..>1036
                     /locus_tag="IV228_002985"
     CDS             35..>1036
                     /locus_tag="IV228_002985"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8718809.1"
                     /translation="MKKIKIMHLLQSNRFSGAENIVCQIMSLFQNNEKFEMIYVSPDG
                     PIANILNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSH
                     LHNNPLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNT
                     ERIIKMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLF
                     DEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPV
                     VAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNIN
                     "
BASE COUNT          399 a          114 c          190 g          333 t
ORIGIN      
        1 gtaagtaaat aattgaattg aaagtgatat taatatgaaa aaaataaaaa ttatgcattt
       61 gttgcaaagt aatagatttt ctggcgctga aaatattgtt tgtcaaataa tgagtttatt
      121 tcaaaataac gaaaaatttg agatgattta cgttagccca gatggtccta tagcaaatat
      181 tttaaatatt agaggtttaa gttacttacc attaaaaaaa ttttcgagaa aagaaattga
      241 cagagccgta catgaattaa acccagatat aattcatgct catgatttta atgctagtgt
      301 aagagcaagt agatataaga agcaaatcat cctttcccat ttgcacaata atcccttgtg
      361 gttacctaaa ctagacataa gaactattat atattcttta tgttcgtcta ggtttaattc
      421 agtaattggt gtttcgaatt ccgttaagga agaatttcgt tacaaacaat taataaaaga
      481 taaatttgtg ttgttaccta acactgtaaa tactgaaaga attataaaaa tggggaatga
      541 atttgttgaa gatgatattg atattttata tgtaggacgc atggcagaac ctaaaaatcc
      601 aataggtgca attaatgttt tagaaaatgt agtaaaaaaa tattcaaaac atttacgtgt
      661 cgtgatgatt ggtgaaggac cattatttga tgaagttaag atgcaaatac aaaaaagaaa
      721 cttaacagat gttattgagt taaagggatt caaaagtaat ccctataagt acatgaagaa
      781 atctaaagtt ctattaatgc catctctttg ggaagggttt ggcctagtat cagtagaggc
      841 aatgttgtta gagacgcctg ttgtagctta taacgtagga ggattgaagg atattatcga
      901 tgagacaaat ggatttctgt gtggtagtga agttgaatta tctaattcac taatgaagct
      961 attaaattca gaagaactta gaaaaaaaat gggagaaaat gctgctatta gtgcaaagaa
     1021 attttctaat ataaat
//
LOCUS       DACDHL010000247         1002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDHL010000247 DACDHL010000000
VERSION     DACDHL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.254"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(81..635)
                     /locus_tag="IV228_002986"
     CDS             complement(81..635)
                     /locus_tag="IV228_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8718810.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          324 a          186 c          107 g          385 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctacatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
      721 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
      781 tattccgact tttttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
      841 tttaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
      901 aaattatatg aaatttaagt ttgaacaata tactaagaac tccccttact ataaagtgaa
      961 ataaattagc atatgaataa ccgtgctacc aaacttttca tt
//
LOCUS       DACDHL010000248         1002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDHL010000248 DACDHL010000000
VERSION     DACDHL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.255"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1002
                     /locus_tag="IV228_002987"
     CDS             <1..>1002
                     /locus_tag="IV228_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8718811.1"
                     /translation="GKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTP
                     FCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCL
                     LFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIV
                     TDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYR
                     QLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDL
                     LTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH
                     "
BASE COUNT          300 a          209 c          191 g          302 t
ORIGIN      
        1 ggcaagaaga tctctatgat tctcctgaat cgtagcggta ataaacggac ctatcttcgg
       61 ttgaagaaac agcgatacct ctgccgagca tgtaaaaaat attttaccgc tcggacgtat
      121 ctcgttaccc cattctgctt tatttctaag cagattcatt acaagatctt agaagaattg
      181 acagagcgtc aatctataaa agcaattggt aaacattgcg atgtttctgt gacaactgtt
      241 cagcggacat tgtccgcctt gtcatcagat atccgtcaga atcttgagta tttacctcgg
      301 tgtctattat tcgatgagtt tcgttctctt tctacatggc acggtaaatt ttctttttct
      361 tgtatggacg gtgagaccgg gaaactgttt gagatcttac ctagccgccg taaaaaagat
      421 ctcgttgctt ttttcatgcg tttttctctc aaagcgaggt tgggtgtaca ttacatcgta
      481 acggatatga acgctcctta tttctcacta gttaaagagt gttttccaaa tgcacaagtg
      541 atcgtcgatc ggtttcacat tgttcaacat ctgaaccgct tcttcgatgc ggttcgaaaa
      601 cgtgtgatga aaacacttga tcaaaaagac ccaatacaag ctaaacagta tcgccaattg
      661 aaatccttat tcaaattact tttaaaatca gaggatcgtt tagattacaa tacactaacg
      721 aaaagacgca atttcaattg gagactattg actgaaacag aagtagttga ccgccttcta
      781 aagatttctc ctgaacttaa aacagcttat cgttactacc aagacctgct tactgcgtat
      841 caggaaaaag atccagatac ttttttccag ctgcttcgag caatgcctgg agaagttcca
      901 ttggaactgc accatatcaa aaaagccttt ttcaaatacg agaaagggat tcggctggca
      961 ttactgaaga agtattcgaa tgcacgactg gaaaatctcc at
//
LOCUS       DACDHL010000249          995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDHL010000249 DACDHL010000000
VERSION     DACDHL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.256"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..893
                     /locus_tag="IV228_002988"
     CDS             <1..893
                     /locus_tag="IV228_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8718812.1"
                     /translation="TVTPRNYKLNFRKVNDLDTHYAMIGIAAGIADYPLYYDATNEKG
                     LSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGETKKLLKNINLANINYS
                     DELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRVLS
                     SETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQ
                     FFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDL
                     DSHKLISYPIIEKQQIKYIN"
BASE COUNT          351 a          127 c          171 g          346 t
ORIGIN      
        1 ttactgttac tccaagaaat tataagttga attttcgaaa ggtaaatgat ttggatactc
       61 attatgcaat gattggtatt gccgctggta tagctgacta ccctctttat tacgatgcga
      121 caaatgaaaa aggattgagt atggctgggc taaatttttc tgggtatgct gattataaag
      181 aaatacaaga agggaaagac aatgtatctc cttttgaatt tattccttgg attttaggac
      241 aatgctcaac agtaggagaa actaaaaaat tgttaaaaaa tatcaattta gcaaatataa
      301 attatagtga tgaacttcct ttatcccctt tacattggct attagctgat aaagaaaaat
      361 caattgtcat tgaaagtatg aaagatggac ttcatatata tgataaccct gtgggcgttc
      421 ttaccaataa tccttcattt gactatcaat tatttaattt aaacaattat cgtgtcttat
      481 cgagtgaaac tcctaaaaat aatttttcaa atcaaataag tttgaatgcc tatagccgcg
      541 gtatgggagg gataggcttg cctggagatt tatcctcagt atctcgtttt gttaaagcga
      601 cttttacgaa gctgaattct gtatctggag attcagagtc agaaagtatt agtcaatttt
      661 tccatatctt aggttcagta gaacaacaaa aaggtttgtg tgatgttggt gatggaaaat
      721 atgaatatac aatttattct tcttgttgca atgttgacaa aggaatctat tattatcgaa
      781 catatgaaga cagtcaaatt actgcaattg atatgaataa agaagactta gatagtcata
      841 agttaattag ttatccaatt atagaaaaac aacaaattaa atatataaat tagttaatgt
      901 gttgtgattg attatttaat atagatataa tacaaaaagg caaagatatt ttcaaaacat
      961 tttttcttga tgtgatatcc ttgtcttttt tgtaa
//
LOCUS       DACDHL010000250          993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDHL010000250 DACDHL010000000
VERSION     DACDHL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.257"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..367
                     /locus_tag="IV228_002989"
     CDS             <1..367
                     /locus_tag="IV228_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8718813.1"
                     /translation="KKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVG
                     LYTKTEHRTVKYMNNLIEQDHRPIKRRNKFYRSLRTASSTIKGMETIRGIYKKNRRNG
                     TLFGFSVSTEIKVLMGIPA"
     gene            450..>993
                     /locus_tag="IV228_002990"
     CDS             450..>993
                     /locus_tag="IV228_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718814.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRR"
BASE COUNT          341 a          142 c          203 g          307 t
ORIGIN      
        1 aaagaaacgg gatacgcaag cagcctatgc tttcttaaaa cgactccata aacagtttgg
       61 tgagccgaaa gcaattgtga ccgataaagc accttctctt ggctccgcct ttagaaagtt
      121 acagagtgtg ggtttatata ctaagacaga gcaccgaact gtgaagtata tgaacaattt
      181 aatagaacaa gaccatcgac ctattaagcg acggaataaa ttttatcgaa gtctccggac
      241 tgcctcatcc acgattaagg ggatggagac cattcgagga atatataaaa agaaccgaag
      301 aaatggaacg ctcttcggct tttcggtgtc tactgagatc aaggtattaa tgggaatacc
      361 agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc gcaacagaac
      421 cattatttta aagtgggagg aaaattgttt tgtggatatt taaacaagaa atctttgcta
      481 aattatttag tttagatgga ttaggttggg gaatcttatt ttttattttt ttatttttag
      541 gatctatgtg gatctggttt gttgataaaa aaaattctta tgagaaagta atagtgaaaa
      601 aaatgaatcg aaatgaattc gatgattttt caaaggaaca tagagttgaa ttgtacgata
      661 gtcatgaaat taacagtact tctccatctt attattgggt atttggttgc gaagaaaaac
      721 cattggagat taaattgttg aaaatgcttg aaaatcctcc agtcattgaa gatttttctg
      781 caaaaaacgt tgaaaaacag atgtttgaac aggtacaaat aagaggtcaa aacaaaattt
      841 cattagaacc agatgaatat gcagcctttt ttttagaaac aaattcaggt acttcaatgc
      901 ctgttttcaa agttaaatta aaaatgaatg gtcgtttatg cacttatgat cttgttgcaa
      961 atatgcgcta tggttctact ggtgatagac gta
//
LOCUS       DACDHL010000251          992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDHL010000251 DACDHL010000000
VERSION     DACDHL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.258"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            359..992
                     /locus_tag="IV228_002991"
                     /pseudo
     CDS             359..992
                     /locus_tag="IV228_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          339 a          181 c          182 g          290 t
ORIGIN      
        1 agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa caccttcaat
       61 tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta
      121 ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag tgcaaatatt
      181 aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga tctatatatt
      241 catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata taggctctat
      301 aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt ttaggtatat
      361 gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc
      421 aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata aaatgagctt
      481 tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac gaaaattgtc
      541 tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac
      601 agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt
      661 cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca
      721 agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag tgaaaaagaa
      781 ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca aaacatggcg
      841 cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg atcgattgct
      901 ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga ccgaagcctt
      961 tagagacaag gaatcagact tattttttac ct
//
LOCUS       DACDHL010000252          984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDHL010000252 DACDHL010000000
VERSION     DACDHL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.259"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            271..963
                     /locus_tag="IV228_002992"
     CDS             271..963
                     /locus_tag="IV228_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8718815.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          425 a          141 c          164 g          254 t
ORIGIN      
        1 aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt gcttttttgt
       61 ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc tctataaaaa
      121 agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta tttatattaa
      181 aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta cttttagcat
      241 gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac tggtgggatt
      301 gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa tgaaattatt
      361 ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca gcttgatcat
      421 acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc acaaaaagaa
      481 cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt aaatgaatta
      541 gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca attagtcagc
      601 aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga tgagatcaac
      661 caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact acaagaagca
      721 caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca aatcgcaaca
      781 ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa gaaattagac
      841 gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga aaaagcaaaa
      901 gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca tttgcgtaaa
      961 taatctttct taacaagtaa acct
//
LOCUS       DACDHL010000253          982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDHL010000253 DACDHL010000000
VERSION     DACDHL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.260"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>982
                     /locus_tag="IV228_002993"
     rRNA            <1..>982
                     /locus_tag="IV228_002993"
                     /product="23S ribosomal RNA"
BASE COUNT          305 a          195 c          284 g          198 t
ORIGIN      
        1 ccgaatgggg gaacccaaca tctttaatag gatgttacga ttgtgtgaat acatagcaca
       61 ttcgaggtag acgcagagaa ctgaaacatc taagtacctg caggaagaga aagaaaattc
      121 gattccctga gtagcggcga gcgaaacggg aaaagcccaa accagcaagc ttgcttgttg
      181 gggttgtagg actccaatat ggtagttctt tcagatagtc gaatgacttg gaaaagtcag
      241 tcaaagaggg taaaaacccc gtagacgaaa tgtggaagac acctaggagg atcctgagta
      301 cggcggaaca cgagaaattc cgtcggaatc cgggaggacc atctcccaag gctaaatact
      361 ccctagtgac cgatagtgaa ccagtaccgt gagggaaagg tgaaaagcac cccggaaggg
      421 gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtc aaagcccgtt aatgggtgat
      481 ggcgtgcctt ttgtagaatg aaccggcgag ttacgattgc atgcgaggtt aagttgaaga
      541 gacggagccg cagcgaaagc gagtctgaat agggcgtttg agtatgtagt cgtagacccg
      601 aaaccatgtg atctacccat gtccaggttg aaggtgcggt aaaacgcact ggaggaccga
      661 acccacgtac gttgaaaagt gcggggatga ggtgtgggta gcggagaaat tccaaacgaa
      721 cttggagata gctggttctc tccgaaatag ctttagggct agcctcggaa ttgagaatga
      781 tggaggtaga gcactgtttg gactaggggc ccatctcggg ttaccgaatt cagataaact
      841 ccgaatgcca ttcattcata tccgggagtc agactgtgag tgataagatc catagtcgaa
      901 agggaaacag cccagaccac cagctaaggt cccaaaatat atgttaagtg gaaaaggatg
      961 tggggttgca cagacaacta gg
//
LOCUS       DACDHL010000254          977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDHL010000254 DACDHL010000000
VERSION     DACDHL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.261"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>977
                     /locus_tag="IV228_002994"
     CDS             <1..>977
                     /locus_tag="IV228_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8718816.1"
                     /translation="TVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGD
                     VEFTLGNITDQVLPREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGE
                     ITFTDQPAYVNSKGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYP
                     KNIVSKLSFELTKFGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTA
                     TDLTLGKYYFVEVPSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLK
                     ASYVNYKAPVIDKTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADG"
BASE COUNT          358 a          158 c          216 g          245 t
ORIGIN      
        1 acggttcata aattaatgta cgatcaagga acgcaattga atgttgatat tgacgggatc
       61 aaaaacgatg gttatacgca cgatcaatat cctacaggtg taacgaaata caacaaggca
      121 gattatggtg atgttgagtt tacgttagga aatattacgg atcaagtatt accgagagaa
      181 gattctgatc taaccaacgc gaaagtggac gagattgtac aagatgtaga agacaaggga
      241 tctaacagtg agtacgtcaa gaatgcaacc aacaaaatta cttcagctgt agatgaaaat
      301 ggtgagatca cttttactga tcaaccagcc tatgtcaatt ccaaagggaa tgtatatgtg
      361 gtttatgaaa gtaaatccgc tgctggatta gtcacacaaa aagcaaaacc aatggttgtg
      421 attgcgccaa tgacagataa aacaggatca ggatttttaa aaaatattca tttgtatcct
      481 aagaacattg tttctaagtt gagttttgaa ttaacgaaat ttggtgacga tggcgcagac
      541 caaagcaaac aaaccctttt aaaaggggca aaatttgagt tatacaaggg cgaacctgga
      601 aaaggaacaa aattgggtga tcttgtatcg gatgatcaag gaaagctgac tgcgactgat
      661 ttgacgttag gaaaatacta ttttgtggaa gttccttcgg aagttgtagt gggatcagat
      721 caagaaccaa cagcagatca atatttatta ggcgcagacg ctcgcaacga tgcgcacaat
      781 aaattaacct ttgaaattac gaatgatggc gtgacaagtg acttaaaagc ttcatacgta
      841 aactataaag caccagtgat cgataaaaca gtaacaaatg gaacaggaca agaacattct
      901 ttccaaattg gtgacgcagt aaattatcaa ggaacgattc atattccaac agacattgca
      961 ggtggcgcag acggtat
//
LOCUS       DACDHL010000255          974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDHL010000255 DACDHL010000000
VERSION     DACDHL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.262"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            123..>974
                     /locus_tag="IV228_002995"
     CDS             123..>974
                     /locus_tag="IV228_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8718817.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEE"
BASE COUNT          345 a          149 c          185 g          295 t
ORIGIN      
        1 ttttacactc tctattatga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agag
//
LOCUS       DACDHL010000256          952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDHL010000256 DACDHL010000000
VERSION     DACDHL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.263"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(278..637)
                     /locus_tag="IV228_002996"
                     /pseudo
     CDS             complement(278..637)
                     /locus_tag="IV228_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          298 a          168 c          182 g          304 t
ORIGIN      
        1 aaatccgtta gaaaatacaa tttgtagata tccaattgat ttgtaaacaa aaaggagttg
       61 tcaactaagc cactcctttt tgttgtaatt tttcccaata agaaagtcta ctactttagg
      121 aagataatag taagatagaa atgttgcccc caaaattatt atccagctgg atataattaa
      181 tcaaataaaa aggatcaaaa atgtagtgac ccccaaaagt tagacttttt tggagtggag
      241 aaatccactc cttttttcat gctctaaaat gatattttta cacaaccgta acggttgttt
      301 tacgaaattg gacgggactt tgacagttta atttctgttt tatccgttca ttattgtagt
      361 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      421 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      481 tccctttccg agacaggctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      541 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      601 tggtctggat agcttcctcc aaaccttcca ttaccgcacg tgtttctgaa tggtggtagc
      661 gaccgatttg aacggctagg gcggaaatac ggtgcaaatg cacggtggtt tcatgcaatg
      721 cttacagggc gtattttgta agggtatgta acggagatga tcccgacggt aatggatgtg
      781 agaggccaag ccgacgctga cggtagagca aacaaacata gttgcactct agcgatcgtt
      841 atttattttt aacggtcaaa ctagggtaca actatgctta aaacacttcg cctcaacttc
      901 tggtagcaaa agtgattttg aaagtcaaaa tattttttat gagttattta ta
//
LOCUS       DACDHL010000257          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDHL010000257 DACDHL010000000
VERSION     DACDHL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.264"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..705)
                     /locus_tag="IV228_002997"
     CDS             complement(37..705)
                     /locus_tag="IV228_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8718818.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          356 a          164 c          159 g          264 t
ORIGIN      
        1 tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact gttcaagagg
       61 atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc
      121 aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt
      181 atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat
      241 caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat
      301 atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag
      361 aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct
      421 ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc
      481 gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt
      541 gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc
      601 atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc
      661 taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc
      721 ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa
      781 aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat
      841 gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg
      901 agtttttttg gctcaagaac aaaaactggt gcctgtgaca atg
//
LOCUS       DACDHL010000258          933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDHL010000258 DACDHL010000000
VERSION     DACDHL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.265"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            225..815
                     /locus_tag="IV228_002998"
     CDS             225..815
                     /locus_tag="IV228_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8718819.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          328 a          144 c          159 g          302 t
ORIGIN      
        1 ggaattggtt cttttgtcat ccatgtatgc aaaatgttat aatgctggta aacaaaaatg
       61 attacctgtc atttttattt agttcgtatt taattaagtt gactgtctgt atactctgtc
      121 taaaacttaa gaattgttgt tttcgctgtc gctatccaaa tttaatgaat gtctgtatgt
      181 taaaactgtt gtccttgtca tagatagtaa aggagagctt gaaaatgaaa actgcgatta
      241 cgtttctatt agaggaatat gccgattggg aaggtgctta tattactagc acactaaacc
      301 aaaaagaggg atggcaaaac aaaactgctt ctatccaatc agaggtcact tccattggag
      361 gattaaaaac aaaaatcgat tacttaatcg aacaaatacc tgaaaaagtt gaccttttta
      421 ttatgatcgg tggcaattca tgggacataa aaaatcatca tttatatcaa ataatcgata
      481 aattgttaaa aacagacgta ccagtagctg ctatttgtgg ggcagttgat tatttagctc
      541 agagcggatt attgcaaggt tacaaacaca ccggaaatgc gcaatttcct tggttagaat
      601 cggaaaatta tcagaataaa gaagatttta tagaacaaca agctgtgaaa gatcgtaatt
      661 tgattactgc gaatggaaca gctcctatag aattcacaga cttgattctt caagcagtcc
      721 attttgaaac agaagaaata agagaaaaaa agattcgtct atacaaactt ggattctatg
      781 attactataa aaagtacgga gatcctttct cctaatcatt ctcttcaatt aaatactcct
      841 tatcaatagt atgacaagta attttactga tggttatttg tcacactttt tctgacattt
      901 atcaaagaag atacactcta tttatattca tag
//
LOCUS       DACDHL010000259          925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDHL010000259 DACDHL010000000
VERSION     DACDHL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.266"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(69..401)
                     /locus_tag="IV228_002999"
     CDS             complement(69..401)
                     /locus_tag="IV228_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718820.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(453..>925)
                     /locus_tag="IV228_003000"
     CDS             complement(453..>925)
                     /locus_tag="IV228_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718821.1"
                     /translation="KRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDS
                     RLNQFKDNDQSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTR
                     EAAPKPIQNVHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
BASE COUNT          259 a          182 c          154 g          330 t
ORIGIN      
        1 cgctcttctg gttttccaaa ctgcaaaatg gatttccttt caactgaatt ttgtcctttg
       61 actcgcattt attcatcctc cttatacgta ccatgataat ttcggttagg atctaacgca
      121 tgataatggt tcttgtccgc aaaaaaaata gactcgataa tcgcaaaaat ttgtcgttga
      181 cctgggttat tatagggtcg ccaaacaatc actaacgcta tcgcaatcca taagccataa
      241 ttcaagagtg caaaaaagag tggtaagtgg acaagattat tgaccatttt cgcccaaaaa
      301 aagcagccac caagcgttaa tagatctaaa aagaaaaaag cgaaaaactt ccattctcca
      361 ctaaatcttg gagggttttt catgttacta tatttatcca taaatgattt ctccttttca
      421 tacatagaaa aaaggcctaa cttgtaggcc ttttatggtt tgatatcctg tttgatatcc
      481 ctcaatagtt gttctttcaa tgcattgttc ctgttttttg tttctcttgt cacatttatt
      541 tttgttggtg cagtaaatgc cacatttgct ggcttgtaac ctgtatgaac gttttgaata
      601 ggttttggtg cagcttctct tgtcacaaca ttttgaacgg acgcattccc tgtctcacca
      661 ctttcagata aattgtaaga aacagttgtc agatttccat tttcaggcaa acgacttggt
      721 ttcatttgaa cagtacctgc aggtgattga tgaacaaaac tttgatcatt atccttgaat
      781 tgattcaaac gactatcttt cattggtaaa ttttgtgcct taggtaaacc aattttcgtt
      841 gatttttcag ttccaacttc gattccatta tcatttggca gaacgatgga atcctcatcc
      901 gtttgtactt cttgatttcg ttttg
//
LOCUS       DACDHL010000260          923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDHL010000260 DACDHL010000000
VERSION     DACDHL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.267"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(113..313)
                     /locus_tag="IV228_003001"
     CDS             complement(113..313)
                     /locus_tag="IV228_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8718822.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          298 a          162 c          144 g          319 t
ORIGIN      
        1 aacatctaaa gtagaaaagg aattctacct tagatgttct tatctcactc tatcagtcct
       61 atttgacaaa gagccctttt ctttcgaaaa gacggttaaa agtcaatttt tattaagctt
      121 taacaatgtt tgttgcttga gggccacgtt gaccatcttc aacatcgtaa gacactgctt
      181 ggccttcttc taaagttttg aagccgtcac cttggatagc tgaaaaatgt gcaaatacat
      241 catttccatc ttcaccagtg ataaagccaa aacctttgtc tgcgttgaac cattttactg
      301 taccgttatt cataaataaa ttcctcctga tacgtatcaa tgtacgtttt ttcgttgcaa
      361 taaatatttg ataggcaaaa ggagttttat ctatgtagat atatcgctca aattacgttt
      421 caaaatagat tactcattta atatatcatg ctttttctaa taacacaagg ataatgcaat
      481 gaaatataaa attcattgtc ttctattgtc ttcgaatcga agaaaaagtt gaataaccgc
      541 gattgattca ctttttgttt tcttgtttat ttgcttttat ctgcaacttt tgactttaaa
      601 atttcttaac tatgaaaacc tttcaattgg tggtatactt ataaatggaa tttcgttatc
      661 aacgcgttcg ttgcctttca gcaagtattt aaagaaagga gaatggctat gacattctat
      721 aacaaagata tacaatatcg agtaaccttg gatacagatt tgaatctatt tgtcgttttt
      781 gacaaagaaa ataccaatca tgtagcgaca ggtgttacga tcgaacaagc agtgcaagaa
      841 ttaaaaaaat ctgcataagg agtggaaacc ctcatatgga cgattctcgt tcttctcttt
      901 aaacgcatct actggtagat aac
//
LOCUS       DACDHL010000261          866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDHL010000261 DACDHL010000000
VERSION     DACDHL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.268"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..330
                     /locus_tag="IV228_003002"
     CDS             <1..330
                     /locus_tag="IV228_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718823.1"
                     /translation="HEDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATI
                     DLNEELVKKLKDQLPYQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKID
                     RMTKEKI"
     gene            352..582
                     /locus_tag="IV228_003003"
     CDS             352..582
                     /locus_tag="IV228_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718824.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT          375 a          128 c          144 g          219 t
ORIGIN      
        1 cacgaagata aggttaaaga tcaagtgaaa gatgaagatt atgaacaaca cgcgatcaat
       61 tacaaagtga aaggatacaa gaaagaacaa aaagcagctc aaaaacaaat ggaatctttc
      121 caagcaacca ttgatttgaa tgaagaatta gtgaagaagc taaaagatca attgccttat
      181 caagtcgcag atgatcagga aaatactgaa aacaagatta atagctacca gcaagaaatt
      241 gaaacatcga aaacgcaact gaaagatcaa gaagagatca ttcaaaaact tcaagaaaaa
      301 attgatcgta tgacaaaaga aaagatttag aaggacaggt ggagtgaaaa aatgaagcga
      361 acaaacttgg ctgacaaaga tactttcgct gagcagaaag tattgattaa agcctatctc
      421 aaagttgtaa aagctaaaga aatatgtcga gaagtgaatt tatctaacac aacaatatct
      481 gagttgaaga atggtcgaaa gaatttagat actttaaaat tcagatcatt tgaagctcta
      541 tatgtagctg ctaaaaaata ttttacagct gataacaaat aaaaaacagg agtattttat
      601 gtgaaaaaag tatataaaaa atgcttatga atcagctata tcaccttaac catttgttaa
      661 tgagatggga gaaaacatta accacgattc taacgcaaac atcaagcaca taaaacattt
      721 aacactcctc ctttctggct gaagatttca accaaacagg aagacagcgt atcaattata
      781 gcatacactt tttcttaaat tcatttttca tatgacaaaa tatttccttc taatcgacaa
      841 agttagaagg attttttatt gtctaa
//
LOCUS       DACDHL010000262          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDHL010000262 DACDHL010000000
VERSION     DACDHL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.269"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..412)
                     /locus_tag="IV228_003004"
                     /pseudo
     CDS             complement(1..412)
                     /locus_tag="IV228_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            488..643
                     /locus_tag="IV228_003005"
                     /pseudo
     CDS             488..643
                     /locus_tag="IV228_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            662..>863
                     /locus_tag="IV228_003006"
     CDS             662..>863
                     /locus_tag="IV228_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8718825.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNV"
BASE COUNT          266 a          123 c          162 g          312 t
ORIGIN      
        1 acgatctctt ctaagtcgga ctcattttcg atcggtttcg cttgataata gtaggtttga
       61 cgagaaatat ttagaatttt gcacatcgct gatatggaat atttatgctt gttggcatca
      121 attacttgtc ttttcgtccg aatatcagcg ccgcttgctt taaaatatca ttctccattt
      181 cgagttgctt atttttcttt cttagtgcga tcaattctgc ttgttctggt gttaagttgt
      241 ctctttcttt gaatgagccc gttgattgtg cttgcttcat ccatttatcg aaagaagaag
      301 gcgtaagctc atactctcga ataattcctg cgcaaggctt accagcgaga tagagatcga
      361 cgatttgttg tttgaattct tttgaatagg ttcttcgttg acgtcttggc atgaaaaatc
      421 ctccagtgtg ttttttatta ttctacacac cttatttttt ctgtctagtc tagtgtagcc
      481 gattcaggat aaggataagt gtattaattg tggaaaatgt aaaaaagttt gccaaatgaa
      541 taaagattca gttgagaatt gcaatcatct ggagtgtatc agatgtggac gatgtaaaaa
      601 tgcttgtcca gtggatgcaa ttagttatgg agtaagaaaa tagaaaggag tagttaggaa
      661 agtgataaaa aatgctatag catatattac aagaaagaaa aatagaactt taataatttt
      721 tatcatatta actatagttc tttcttgctt gtattcttgt ttaacaataa tgaaatcaag
      781 caatgaaata gaaaaggctt tatatgaaag ttctaattct tcaatatcaa taacaaaaaa
      841 agatggtaaa tattttaatg tta
//
LOCUS       DACDHL010000263          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDHL010000263 DACDHL010000000
VERSION     DACDHL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.270"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>863)
                     /locus_tag="IV228_003007"
     rRNA            complement(<1..>863)
                     /locus_tag="IV228_003007"
                     /product="16S ribosomal RNA"
BASE COUNT          187 a          265 c          191 g          220 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc att
//
LOCUS       DACDHL010000264          853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDHL010000264 DACDHL010000000
VERSION     DACDHL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.271"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            411..853
                     /locus_tag="IV228_003008"
                     /pseudo
     CDS             411..853
                     /locus_tag="IV228_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          305 a          148 c          156 g          244 t
ORIGIN      
        1 tttatcacgg atgagcttga aatcgtcttt tatacgtatc aaattgaaat gttcggcata
       61 caacctatcg ttgttactac attactaatt gtcacttgtt aaggaacttg gatcctttct
      121 aaacattccc tagagataaa ttttacttta tccctccgtt aatatccaat ttaagcacct
      181 ttccgattca gggttaattt gcatatcaag tgcagcttgt gttctagtaa gattagccct
      241 tttagtccgg ctgagttgac ttatggatat aagatatggt catagcaaat agatactacg
      301 tatagtctgt taatttagtg tcattaatct taagttggat aggctacaat taactagaca
      361 gaaaaattaa ggtgtgtaga ctagaagaaa acataccagg aggaattttt atgtctaaga
      421 gaacacgaag aactttttca caagaattca agcaacaaat cgtcaatctt tacttagctg
      481 gaaagccacg tgtagaaatc attcgagaat atgaactaac ggcttcagca tttgacaaat
      541 gggtaaagca atctaaaacg agtggttcat tcaaagaaaa agataatcct acgcctgaac
      601 aaaaagaatt gttagaacta cgtaaaagaa accagcaatt agaaatggaa aatgatattt
      661 taaagcaagc agcgctgata ttcggacgaa gagacaagta atcgatgcga ataagcatct
      721 ttaccctata tcagcgatgt gcagaatatt aggtctatca cgtcagtcct attattatca
      781 atcaaaacca aagaaagacg aatcagaact tgaagaagca gtcgctgaag aatttatccg
      841 cagccgaaag gcc
//
LOCUS       DACDHL010000265          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDHL010000265 DACDHL010000000
VERSION     DACDHL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.274"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(64..363)
                     /locus_tag="IV228_003009"
     CDS             complement(64..363)
                     /locus_tag="IV228_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718826.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(369..599)
                     /locus_tag="IV228_003010"
     CDS             complement(369..599)
                     /locus_tag="IV228_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718827.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          278 a          138 c          103 g          274 t
ORIGIN      
        1 atatctcctt tcataagtat ggtgataaaa tccgttccgg gctacctttt taggtagcct
       61 actttaatct ttgtatcttt ctggatcaac gaaagtatac tttatatagt cataacgccg
      121 atgatcgctt cgagcgtcta gcacatcagt cacgatatca aacaaaaaat atacgtcttt
      181 cttcattcta gttttcgcag cagggatttt aaagtagttc ttattagaat agtagagatt
      241 aattaataag ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa
      301 atctttgaca aatctattcg aaggatcaaa tttaaaccat aattgcgtct cattaaaaag
      361 cataaccatt actccgatct atttttaaac ttagtctcta cctctaatat atatcgagtt
      421 tttattttgc cttcagagaa tactgtttct ttttttgcag ttacaggttg tttattttcg
      481 gaaaaagcta atatagctaa aattgaaaca tccatctgga atttgtcttt ttcgttgctt
      541 tgctcataaa aatctgcata ttcatcacta atattttttc taataaattc ttccatcata
      601 aagatcacct cgaaaagagt atacgaacaa acgttctttt tgtaaaggtg gaatttatta
      661 tactaaataa ataggtgaac aagtacttgt gccaaattat gtgccaaaaa aaatcgaatt
      721 taataaaaca caaacaaaaa gaaatcctat tatgataaca tttcttataa taacaaacac
      781 aacagaagac gtg
//
LOCUS       DACDHL010000266          789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDHL010000266 DACDHL010000000
VERSION     DACDHL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.275"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..183
                     /locus_tag="IV228_003011"
     CDS             <1..183
                     /locus_tag="IV228_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8718828.1"
                     /translation="FSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSP
                     FEPAVYRYEITKPVGG"
     gene            185..463
                     /locus_tag="IV228_003012"
     CDS             185..463
                     /locus_tag="IV228_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8718829.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            444..770
                     /locus_tag="IV228_003013"
     CDS             444..770
                     /locus_tag="IV228_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8718830.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
BASE COUNT          281 a          129 c          154 g          225 t
ORIGIN      
        1 ttttctgcat ttaaaattca agaagttatt ggtgccttgg aaattcaaaa acttgttgaa
       61 aaattctctg gtaaaaatca aattggattc caaatttaca acttgctaga tggtcaattg
      121 gtttattctc catttgaacc ggcagtatat cgctacgaaa ttacaaaacc agttggtggt
      181 taagatggaa gagcaaacta aagaattgtc tttagaggaa aaattcaaat cacatattca
      241 ttttgaagag ggcatggatg attctttgct ctctttttat ttaaatatgg caaaagatta
      301 tgtcaaaaca gcaactggtg gccaacaaga atatcttatt ttgatggttg ccggcattgc
      361 ctatgaatat agagtttcag aggatgaact cgacaaagct atgaatgcca tgacgccatt
      421 tatcgtgcaa ggagcgattc aaaatgccga agagacagac taataatctc agatggaaag
      481 ccgaattgct agacatcaaa acaggaacag acggaaacga tcgtccaact acaatttacg
      541 aatttaagcg tctaatattc tatgaagaac tcggtgtgac ttctcaagaa aaatatttat
      601 cacagcaagc caagacagac gttgtcagac gaattaaagt cagatgggat aaatccatca
      661 cagagaaatt aagtgcgctc aaaattgatt ctgtaacgta taacattact cgcatttata
      721 cgaatcccga tgcaagagaa atggagttga gtttagctta tgtcgattag ctttgatgaa
      781 ttgaaaaca
//
LOCUS       DACDHL010000267          765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDHL010000267 DACDHL010000000
VERSION     DACDHL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.276"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(597..740)
                     /locus_tag="IV228_003014"
     CDS             complement(597..740)
                     /locus_tag="IV228_003014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718831.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            659..>765
                     /locus_tag="IV228_003015"
     rRNA            659..>765
                     /locus_tag="IV228_003015"
                     /product="16S ribosomal RNA"
BASE COUNT          315 a          124 c          134 g          192 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag
      721 tcgaacgctt ctttttccac cggagcttgc tccaccggaa aaaga
//
LOCUS       DACDHL010000268          754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDHL010000268 DACDHL010000000
VERSION     DACDHL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.277"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..222
                     /locus_tag="IV228_003016"
     CDS             <1..222
                     /locus_tag="IV228_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8718832.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            403..>754
                     /locus_tag="IV228_003017"
     CDS             403..>754
                     /locus_tag="IV228_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718833.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIF"
BASE COUNT          278 a          134 c          135 g          207 t
ORIGIN      
        1 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
       61 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      121 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      181 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
      241 gattttattc tttatttaaa actttgcaac agaaccaccc gcactatgcc attacattta
      301 tatctacgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
      361 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
      421 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
      481 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
      541 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
      601 agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa agtcactctt
      661 ggttggacgc ctgacgtcat tatcgggcga aaagaaaggt ctgtttcatg cggtatgaga
      721 acactttatc gtttattttc taaaggaata tttg
//
LOCUS       DACDHL010000269          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDHL010000269 DACDHL010000000
VERSION     DACDHL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.278"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(137..427)
                     /locus_tag="IV228_003018"
     CDS             complement(137..427)
                     /locus_tag="IV228_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718834.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
BASE COUNT          311 a          115 c          113 g          202 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg g
//
LOCUS       DACDHL010000270          737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDHL010000270 DACDHL010000000
VERSION     DACDHL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.279"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..367
                     /locus_tag="IV228_003019"
     CDS             <1..367
                     /locus_tag="IV228_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8718835.1"
                     /translation="FGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFG
                     RVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGK
                     ENWNDLYHANEVLKIAEEK"
     gene            368..>737
                     /locus_tag="IV228_003020"
     CDS             368..>737
                     /locus_tag="IV228_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8718836.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNL"
BASE COUNT          284 a           81 c          132 g          240 t
ORIGIN      
        1 atttggtttc cctaaatcag taaaaaaaca gtttggtaaa tattcgaatc ttgaaattaa
       61 cagtaatgat ttagcaacat atatattgag atacgatgat aaaattatag aaattcattt
      121 agattatttt ggaagagttc ctacaagaaa atttcaattg tttactttag atgaatgtat
      181 caatgttgac ttattaaata ataaaataag ttttgaagaa aacattgtta aagaaatgcc
      241 tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa acgtagtaac
      301 aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa tagctgagga
      361 gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg ggtttaaaaa
      421 taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag caatgataga
      481 tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata cggacagtaa
      541 tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag aacgtaaacc
      601 tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat tcttacaact
      661 agaaagagaa gataatatat ttgatttaat tatagattta gatttgactt cacctttacg
      721 tgaagtttct aatcttg
//
LOCUS       DACDHL010000271          728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDHL010000271 DACDHL010000000
VERSION     DACDHL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.280"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            135..335
                     /locus_tag="IV228_003021"
     CDS             135..335
                     /locus_tag="IV228_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8718837.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            347..673
                     /locus_tag="IV228_003022"
     CDS             347..673
                     /locus_tag="IV228_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718838.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
BASE COUNT          313 a           75 c          130 g          210 t
ORIGIN      
        1 agtaaattcc aaactattat agcatgaaaa ccacaaattg agtaatattt taattgatat
       61 tactcgtttt gtggtttaaa atgtgcttat taagataaaa accacaaagt gaataatttg
      121 aaaggaggcg ctgaatgata actcttaatg atcaattcat tagaagctta agaagacata
      181 gagccgatct tatacttact aaaaacgatg cagcaaaatt gataggtata aatcggaaaa
      241 catatgtcaa aatcgaaaat gggagcaaag aaagtattag agcatctact tatcaaaagt
      301 tggtaaattg gttacttaat gatttgaaaa tctaaataaa tacaacttgg cgagttggct
      361 gattgaagaa atagaaaatg agaggagaaa aataatggat gcaggaatta cagtaatgtt
      421 agataaacaa caaacgaatc aattgaaaaa ttatgttttt gaaatgacaa aagaggctat
      481 tgatcaagca agaattgata ccggattaga aagaccattt ctaaaaggaa aagagatggc
      541 taaatatttg aatgtatcat atacaacttt tttgaaattt aaaagaatgg gattgccagt
      601 tattttatta gaaaaaatgg agttgttttc aaaagaagaa tgcaaaaaat ggatattgag
      661 tcatcaaatt taaaaaacac ctcgtttttt aaatttgatg aagtagaaaa aattaaaaag
      721 agtgttcc
//
LOCUS       DACDHL010000272          717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDHL010000272 DACDHL010000000
VERSION     DACDHL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.281"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..658
                     /locus_tag="IV228_003023"
     CDS             50..658
                     /locus_tag="IV228_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8718839.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT          262 a          141 c          134 g          180 t
ORIGIN      
        1 agttaatagc gtcgaaagaa taaaaaacgg agaaaaaaag gagtttaata tgaattcatt
       61 taagaaaatt gttttaggta caacttttgc tgctggtgct actgcaatgt ttgtaggaac
      121 tacaaatgct cacgctgatg aagtttatac agtaaaatca ggtgattcat tatctaagat
      181 ctcacaaaaa tttgcaggag acaactcaat gatcgatgca attgctgaaa aaaattcgat
      241 cgcaaacatc aaccgcattt atgttggtga acaattaaca atcccaactt caaatgattc
      301 ttcagcaaca acagaaaaca aaacaacaga aaatacagca tctacaactg aaacagcaac
      361 gcaagaacat acatacgttg cacctgttga aacagttgaa gtagctcctg ctgccccagc
      421 tgctgcaaca gcgccaactt caagctctgc aaaagaatgg atcgcacaaa aagaatcagg
      481 cggttcatac acagctacta acggacgtta catcggccgt tatcaattag atgcttcata
      541 cttaaacggc gactattcag ctgccaatca agaacgtgta gcagaacaat atgtaacatc
      601 tcgttatggt tcatgggatg ctgctaaaac attctggtta gcaaacggct ggtactaagc
      661 cgataaaagg aaaatgatga aaccgagaaa cttgtttatt caagttctcg gtttttt
//
LOCUS       DACDHL010000273          717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDHL010000273 DACDHL010000000
VERSION     DACDHL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.282"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..483
                     /locus_tag="IV228_003024"
     CDS             <1..483
                     /locus_tag="IV228_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718840.1"
                     /translation="GVLITREDHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYV
                     PTIKLVSLTTETDLTEQIVQKRQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRA
                     EQERPDLSFFFVIEGKTIKDLQTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE
                     "
BASE COUNT          287 a          110 c          146 g          174 t
ORIGIN      
        1 ggagtcttaa ttactagaga agatcatttt caacgttatt atcgtgtagt ttctacagac
       61 gtggaaggat taaatgaaca agagaaacta gaacgaatga atcagttgac caccgtcatg
      121 cgtacgtatg tgccaacaat taagttagtt agtttgacca cagagacgga tctaacggaa
      181 caaatcgtgc aaaagcggca attattagat aaaaatcgta tggaacagtc cttaggaaga
      241 aatctacaac gattaaaaaa atatgaaaag aaattagtcg aagaaatcca ggaattgaaa
      301 cgagcagaac aagaacgtcc tgacttaagc ttctttttcg tcattgaagg aaagacaatc
      361 aaggatttac aaaccagaaa ccgtcagtta atgcgatcta gtggcatttt agggttaaaa
      421 ccactctaca aaaaagaatt gatcaagatt ctgtatcgaa tgaacaatat gaatgatgag
      481 taggaatgga gaagagagaa tgaaatatcc gttgatatgg ttagaaaaag atgttccgaa
      541 ggaagagacc actagaaaat ttcctcacac tttaagcctg ggtaagttgg gaaaagggca
      601 atcttttaac caagtaaaga atcaaacaag aagaggtgac tgtacatgtt agaagcaatt
      661 aaaacagctt tttcaccaca aaatgatcaa aaagtacttg atccattaaa gcaacta
//
LOCUS       DACDHL010000274          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDHL010000274 DACDHL010000000
VERSION     DACDHL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.285"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(74..649)
                     /locus_tag="IV228_003025"
     CDS             complement(74..649)
                     /locus_tag="IV228_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8718841.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          240 a          153 c          125 g          198 t
ORIGIN      
        1 cttaatttga caaataataa aatgaaaaaa cagagccaga ttagcattat tttgctaatc
       61 cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgg ggcaacatag
      121 tgatgactaa tgaaacaacg acgatagaca acaagcgatc aaggtagtct gtacccgcct
      181 gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc tgaacgatca
      241 tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc gaagaaacga
      301 tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg tttttatctt
      361 tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt tggattggcg
      421 aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg gacaatcctg
      481 tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg atagaatcga
      541 gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg atcccaatac
      601 acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt ttgttagccc
      661 atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DACDHL010000275          705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDHL010000275 DACDHL010000000
VERSION     DACDHL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.287"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            111..>705
                     /locus_tag="IV228_003026"
     CDS             111..>705
                     /locus_tag="IV228_003026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718842.1"
                     /translation="MVLKQGKQDYQIIGYLEDDLNKIKINSDDVMYFFMTPLSDFPSS
                     VIDNVEGIQIYSNTLTKFGFIDDINESHSTRAQSLKEFLDPYIIVCTPKIKRNHEQAR
                     NYYQGDDLELIPKPKSEVFKNLIKENESLVPIPVFSINNSDSVIKDETSFENQLLNGK
                     LLGKIINFSKDNEDYPLNVFWENTDGSRVLFGEIIGQN"
BASE COUNT          290 a           90 c          104 g          221 t
ORIGIN      
        1 ctttcaatta attcccagaa tgaaaaaaaa gattatacaa agtttaaaaa ataaaataaa
       61 aattcaagaa ttaaatgatt ttactatgct tatgaaggag gaaaatataa atggttttaa
      121 aacaaggtaa acaagattac caaattattg gttatttgga ggatgattta aataaaatta
      181 aaataaactc ggatgatgta atgtactttt ttatgactcc tctttcagat ttccccagta
      241 gtgtgattga taatgtagag ggtatacaaa tatatagtaa tacgcttact aaatttgggt
      301 ttattgatga tataaacgaa tctcatagta ctcgtgccca atcacttaaa gaatttttag
      361 atccttatat tatcgtttgt acaccaaaaa ttaaaagaaa tcatgaacaa gcaagaaatt
      421 actatcaagg tgatgattta gaattaatcc ccaaaccaaa gtctgaagtt tttaaaaatt
      481 taataaaaga gaatgaatca ttagtaccta taccggtctt ttctataaat aacagcgatt
      541 ctgtaatcaa agacgagaca agttttgaaa accaattact aaacggcaaa ctactcggaa
      601 aaattataaa tttctcaaaa gacaatgaag attacccttt aaatgttttt tgggagaaca
      661 ctgatggatc aagagtttta tttggtgaaa taatcggaca aaaca
//
LOCUS       DACDHL010000276          659 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDHL010000276 DACDHL010000000
VERSION     DACDHL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.290"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>659)
                     /locus_tag="IV228_003027"
     CDS             complement(<1..>659)
                     /locus_tag="IV228_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8718843.1"
                     /translation="VTYLHKTKRDWQSMFTMFDEQAEWFGNIKGQSLPSLNIGGGFPF
                     HHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMMGAGKSTVLKMIAEA
                     HLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIFATRTYDEASSLYDD
                     GSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYLNRFYEEYGTVPRGF
                     TS"
BASE COUNT          173 a          152 c          123 g          211 t
ORIGIN      
        1 gaagtaaagc cgcgtggtac tgttccatac tcttcataaa aacgatttaa gtaggtctta
       61 aattctgctt tcatcgttcc ttttagctga ggactcacta actgtacctg atagaccact
      121 ttgtctaagt gtgtttgata ggacgctgct tcattgatct tcaaatcttt gactgatcca
      181 tcgtcatata aactggatgc ttcatcatac gtccgtgtgg caaatatctc taaaggattg
      241 atcattccgt cagatccgtc taaacgcacc ataataccgt tatattcctt taagaaagga
      301 ataaagtctt ttcccttttc aaagccccaa ataaagtctc ctgcagctaa atgggcttcc
      361 gcgatcattt taagaacggt cgatttacca gctcccatca ttcctagaag caaggtatta
      421 gtagacaacc gtacactatt tttatacgtc gcattccaga caaatgggcc gcctgtcatg
      481 gtttccccat attgtgtccc tcctggatct tttagcgttt ggtggtggaa cgggaaacct
      541 ccgccaatgt ttaacgatgg tagtgattgc cctttgatat ttccgaacca ttcagcttgt
      601 tcatcgaaca tggtaaacat cgattgccaa tctcgtttgg ttttatgcag ataagtcac
//
LOCUS       DACDHL010000277          557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDHL010000277 DACDHL010000000
VERSION     DACDHL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.293"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>557
                     /locus_tag="IV228_003028"
     CDS             <1..>557
                     /locus_tag="IV228_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP8718844.1"
                     /translation="QITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFI
                     QIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYI
                     YDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGL
                     LNNVSTRMAIDVANQILLDLPLKQI"
BASE COUNT          200 a           75 c           95 g          187 t
ORIGIN      
        1 tcaaataacg tctatctttt tgaattcttg gatttatcaa atccaattaa catcggacga
       61 taaattatat tccttcatca atgatattaa tagttggtgt agtcgagaag ttcaattagt
      121 caatcgtttt attcaaatag gggatattta tttaacaaaa attcaaaaaa atgttttggt
      181 tattttattt aagttaatgt gtagtaaaga atttcagcaa aaacgtttag cactgatttt
      241 ggctcatgga gatctaacgg caacctgtat agcaagtact gctaatagga tcgttgagag
      301 atatatatac gacgccattg atatgccact aatgacttca agtagtgaga ttgcagacaa
      361 aattaataga tacttacaag agtgtaccgg atatgaagaa attattttac ttgttgatat
      421 gggttcactg gagaaaattt atctaaattt ggaactagac gctcaaaaaa aattcggact
      481 actaaacaat gtttcaacaa gaatggctat tgatgttgca aatcagatat tattagattt
      541 accgcttaaa cagattc
//
LOCUS       DACDHL010000278          549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDHL010000278 DACDHL010000000
VERSION     DACDHL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.295"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IV228_003029"
     CDS             <1..390
                     /locus_tag="IV228_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8718845.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          205 a           95 c           83 g          166 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccatcctaaa acataagcta tttgtttact tctgtagaat
      481 atatactttg ttgttagata agttgcacat gtttatagaa tctaaataat acccgtggtg
      541 ctagttaat
//
LOCUS       DACDHL010000279          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDHL010000279 DACDHL010000000
VERSION     DACDHL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.296"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..476
                     /locus_tag="IV228_003030"
     CDS             <1..476
                     /locus_tag="IV228_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8718846.1"
                     /translation="FVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSK
                     LDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSG
                     IILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          168 a           93 c          111 g          154 t
ORIGIN      
        1 ggtttgtcct agaaaagaaa tggaaagact tttctcctga gaagcgatta agctgtcggc
       61 aacaagaact gcgtccatta tttattgctt tttatgagtg gatggcaacg atagatcctg
      121 ttgcaaaatc caagttggat gcagccgttc aatatgcgtg taaactacga agtggttttg
      181 aacctatttt tgaagatggt cgtttagaac tcacaaataa tcgtgcggag cgaaatatca
      241 aagagctggt gataggacgg aaaaattggt tgcattccac aagtcttgaa ggagcacgaa
      301 cttcaggaat cattctgagt gtatataaaa cagcagaact taacggattg aatcctgtaa
      361 agtatctaga gtttctattt gataagatac caaatcttcc cgtactttca gcagaaactt
      421 tagatcaact gttgccttgg aacaaggatg tacaacaaca tttttctcgt aactaaaaac
      481 gccaaacatt cccgattcta ttctataggt cgggttattt gacgta
//
LOCUS       DACDHL010000280          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDHL010000280 DACDHL010000000
VERSION     DACDHL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.297"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(34..234)
                     /locus_tag="IV228_003031"
     CDS             complement(34..234)
                     /locus_tag="IV228_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8718847.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          169 a           79 c           92 g          169 t
ORIGIN      
        1 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
       61 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
      121 taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga
      181 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
      241 atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata
      301 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
      361 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
      421 tcaaagtacc atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat
      481 agcttagatc ctttattcta tgctttata
//
LOCUS       DACDHL010000281          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDHL010000281 DACDHL010000000
VERSION     DACDHL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.298"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV228_003032"
     CDS             complement(<1..132)
                     /locus_tag="IV228_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8718848.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACDHL010000282          505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDHL010000282 DACDHL010000000
VERSION     DACDHL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.299"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          196 a           64 c           97 g          148 t
ORIGIN      
        1 aaaagtctaa caaggctttt cttttttgtt tatatgtata ttaagatata cgtttatata
       61 aacgtattta tgtatatgaa aatatacgta aatatatacg tggttataaa cattaataaa
      121 acgttaagaa ttgttaaata atgatattta gtaatatacg aattataata tatattttat
      181 ataaaatgta tgtaaataaa taaaatgtct gacggatatt gaatagcaat aaataacaat
      241 aattcagacg gatacggtga gtgaagtagc ttgctacgaa cgaacaggca gaatgaaacg
      301 gcagtttcat tagagcaagt cgcttgcgac gtagcgttac cgctgatgta actggcaagt
      361 tacatagaga gagtttgctt gcaaacgaac gttacctatt tgaatagaaa taaaaaataa
      421 cagattagtg aattttgaat aggagccaca actcaaccaa ccgcttgcgg tgggtgagtc
      481 ccacggagga aaagacgaac gaacg
//
LOCUS       DACDHL010000283          501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDHL010000283 DACDHL010000000
VERSION     DACDHL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.300"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            208..>501
                     /locus_tag="IV228_003033"
     CDS             208..>501
                     /locus_tag="IV228_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718849.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSR"
BASE COUNT          208 a           50 c           98 g          145 t
ORIGIN      
        1 taaagagcat aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc
       61 tattacgttt ctcaaaaata attgtgggaa caaaatattt aaaaatagga tagtgaaagg
      121 atagcaaagg atagtaaaag gatagaagca ggatagcgaa aaggataggc tccttttcgc
      181 tatccttttt aaattggagg aattaatatg gaagtaagaa taagaaatgt agatgagaaa
      241 atcgtaagag aaattgatcg aagatgtgac gaactaagta accgaacggg taataaatgg
      301 agtcgtaacg attatttaaa gctactcgtt gaaaatgatt ttgatcgtcc tttgatggaa
      361 tataaacaag ataaatttga tcaattatta aatcagttaa tagataaatt ttcttttgtt
      421 caaagtcata atacaaaagt attagaagaa tatatttgtc agaataatca aattatcgaa
      481 cttttaattg aacaaagtag a
//
LOCUS       DACDHL010000284          491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDHL010000284 DACDHL010000000
VERSION     DACDHL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.301"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>491
                     /locus_tag="IV228_003034"
     CDS             <1..>491
                     /locus_tag="IV228_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8718850.1"
                     /translation="YDDLNSVLDVTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFG
                     EGVTLLGQFEQSGVDSSAALSSLSKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAI
                     NAAAEVFGTKGGPRMADAIRRGTLNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAM
                     NNA"
BASE COUNT          169 a           86 c          119 g          117 t
ORIGIN      
        1 tatgatgatt tgaattctgt ccttgatgta acaacgaaaa catcgcagaa tactggtcaa
       61 tctgtagacg acttgatgca aaaagcaatt gatggcgcac cacaaattaa acaattaggg
      121 ttgagctttg gtgaaggggt cactttactt ggacaatttg agcaatctgg tgttgattca
      181 agcgcagctt taagtagttt atctaaagca acagtggctt atgcgaaaga cggaaaatct
      241 ttaagtcaag gactagaaga actacaagac aaggtaaaaa atgcaggttc tgaaacagaa
      301 gccatcaatg cagcagcaga agtattcggg actaaaggcg gtcctagaat ggccgatgca
      361 attcgtagag gtactttgaa cctggaagat ttagcaaaaa ctgctggaga aagtggggga
      421 gctgtaggag acacattcga tgctactctc gacccaattg atcaagccga tcaagctatg
      481 aacaatgcaa a
//
LOCUS       DACDHL010000285          483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDHL010000285 DACDHL010000000
VERSION     DACDHL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.302"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          203 a           59 c           82 g          139 t
ORIGIN      
        1 atttatgtgg tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg
       61 tgtaaattta catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta
      121 ttttaaaaaa catataaaat agttgttgac atgttagaaa tgacatgata agataactga
      181 gttgttaaaa aagaaccact tgtgaagctt gattctatcg aaaaaaactt caaaaaagtt
      241 cttgacaaat aacaaatgat aagttatgat aaatgagttg ctgtaagatt tcgaaaaacg
      301 aaatcaaaaa aacttttaaa aaagagttga caattacttg tctctctgat atgatataaa
      361 agttgctacg gcagccaaaa acgaatcggg gaacactcct gatgaagtag acctttgaaa
      421 actgaacaaa gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa
      481 aca
//
LOCUS       DACDHL010000286          470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDHL010000286 DACDHL010000000
VERSION     DACDHL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.303"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(210..>470)
                     /locus_tag="IV228_003035"
     CDS             complement(210..>470)
                     /locus_tag="IV228_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8718851.1"
                     /translation="PKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVR
                     RREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          166 a           59 c           82 g          163 t
ORIGIN      
        1 tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata
       61 aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa gacattagta
      121 ttaaatagtt taaaaaaatg aaaaataata aaggaagtga gtcaattcca gactcctgtg
      181 taaaatgcta tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa
      241 gaataaatcc attcatcatg taggtccata agaacggctc caattaagcg attggctgat
      301 gtttgattgg ggaagatgcg aataatcttt tctcttctgc gtacttcttg attcagtcgt
      361 tcaattagat tggtactctt tagtcgattg tgggaatttc cttgtacggt atattgaaag
      421 gcgtcttcga atccatcatc caatgatgcg caagcttttg aatattttgg
//
LOCUS       DACDHL010000287          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDHL010000287 DACDHL010000000
VERSION     DACDHL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.304"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          149 a           89 c           53 g          171 t
ORIGIN      
        1 gcacaaaaaa taaaaaaatt attacgttat taaaaaaaca gatgaaagat caggttttat
       61 tagatctcat tctttcatca cttgaaggga tttaatgaac tactttttcc tctttgtcgt
      121 ttttatatat tcgtattcct tcaaacagta ggatagcctc tttcaatgct tgatatttga
      181 actgtccgtt acttaaaaat ttagatgtgc cgccctcttc ctagaacata ttctttttta
      241 ctttttcatt aattgacaga ccaatcgaac gaaactgtaa atttcgatag gataatgatt
      301 ggtcaaactc attcattttc aagacatctc gattcatatt caagatagga atctttttat
      361 caacatcact ttattcgtat tttcctctac cataaaagct tcacgctcca caaaattgtt
      421 ttatacacta ctattcagcc cattcaacga ccaatgaatt tt
//
LOCUS       DACDHL010000288          461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.305, whole genome shotgun sequence.
ACCESSION   DACDHL010000288 DACDHL010000000
VERSION     DACDHL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.305"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>461
                     /locus_tag="IV228_003036"
     CDS             <1..>461
                     /locus_tag="IV228_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP8718852.1"
                     /translation="EGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQ
                     WFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRR
                     NGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYS"
BASE COUNT          155 a           87 c           76 g          143 t
ORIGIN      
        1 ttgaaggtga tacgatcgtt ggcattcatc ataaaagtgc cgtcattact ttagttgaaa
       61 gattatctaa agtcattatc acgattaaac ccaacggccg taaggcatta gatattgaaa
      121 ctgcccttaa tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct attacgtttg
      181 actgtggaaa agaattttct aactggaaag ccattagtaa ccaacatgat attgatatat
      241 attttgcgga ccctggaaca ccttctcaac gcccattaaa cgagaattct aacgggattc
      301 tgcgtcgtaa tggactgccg aaatcaatgg attttagaaa agtgaatcag acatttattt
      361 ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa
      421 tcgaaatatt tttgagctat gtacaagaag cattttattc t
//
LOCUS       DACDHL010000289          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.308, whole genome shotgun sequence.
ACCESSION   DACDHL010000289 DACDHL010000000
VERSION     DACDHL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.308"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(36..272)
                     /locus_tag="IV228_003037"
                     /pseudo
     CDS             complement(36..272)
                     /locus_tag="IV228_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          147 a           76 c           77 g          151 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa
      301 aaaagccatt tatgttacac tccaattgta atacccgcta aagcatacaa ggagagtgaa
      361 cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc
      421 atcaagaaac tccggttgct atcgttgcga a
//
LOCUS       DACDHL010000290          431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.309, whole genome shotgun sequence.
ACCESSION   DACDHL010000290 DACDHL010000000
VERSION     DACDHL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.309"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..385
                     /locus_tag="IV228_003038"
     CDS             188..385
                     /locus_tag="IV228_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718853.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
BASE COUNT          165 a           60 c           97 g          109 t
ORIGIN      
        1 tatgaattcg ttgggatgag tgtgcgcaaa aatggacggt tagttattaa atatgcgaaa
       61 gataaccatg caaagcggtt tatgcgagag gagaatcgaa aaattgacca aaaagaattc
      121 aggcaacagt atgaagaaga aaaagaaaaa acttttaaag caattcgctg ggattgggaa
      181 cgccaacgtg aaatttatat gtctaagttg tggtgaaaaa gaatcaatcc caatggatgt
      241 agtaaaattt ttagatgatg cagatattgc aaatgatccg aacaatcctc cccaatttac
      301 ctgtgaaaaa tgtgggaagg agatgtatcc agaatattat agaaacgccc ttggaataga
      361 gtttaagatc agtgatgttc aataagaagg ttaatcatac aaaaagtcca ccggagaatt
      421 cattctccgg t
//
LOCUS       DACDHL010000291          429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.310, whole genome shotgun sequence.
ACCESSION   DACDHL010000291 DACDHL010000000
VERSION     DACDHL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.310"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..>429)
                     /locus_tag="IV228_003039"
     CDS             complement(138..>429)
                     /locus_tag="IV228_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8718854.1"
                     /translation="DFRYHSVSGFITLIFLSLFAGYYFQRNRRIISLFIANIISTVTS
                     YLFCANFTEWRYFYHPLKPTQLILLLAGIYLVPQILGSLWAVALSYKKARHP"
BASE COUNT          173 a           54 c           87 g          115 t
ORIGIN      
        1 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
       61 agttttggtg atataagaaa aacatatatg tataaaaaga agaaagtagc ttctctttgt
      121 acatatatgg tttgttttta tggatgccga gcttttttat aagataaggc taccgcccat
      181 aacgacccaa ggatttgtgg aactaagtaa atacctgcaa gaagcaagat cagctgggtc
      241 ggttttaaag gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa taaataggaa
      301 gtgacagtag atataatatt ggcaatgaat aaagatataa tacgcctgtt cctttgaaaa
      361 taatatccag caaaaagaga taaaaatatc aatgtgatga acccgcttac ggaatgataa
      421 cgaaaatca
//
LOCUS       DACDHL010000292          429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.311, whole genome shotgun sequence.
ACCESSION   DACDHL010000292 DACDHL010000000
VERSION     DACDHL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.311"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>429
                     /locus_tag="IV228_003040"
     CDS             <1..>429
                     /locus_tag="IV228_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8718855.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          132 a           64 c          109 g          124 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggaca
//
LOCUS       DACDHL010000293          426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDHL010000293 DACDHL010000000
VERSION     DACDHL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.312"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>426
                     /locus_tag="IV228_003041"
     CDS             <1..>426
                     /locus_tag="IV228_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8718856.1"
                     /translation="HLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKT
                     EYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYG
                     HHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          156 a           78 c           71 g          121 t
ORIGIN      
        1 gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt ttttagatta
       61 cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt cttactttcg
      121 atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc gtaatggtga
      181 gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg aaaccactat
      241 tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga tcgaaaaaat
      301 gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc tgactgaaga
      361 agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt ttatggacgc
      421 tactta
//
LOCUS       DACDHL010000294          420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.313, whole genome shotgun sequence.
ACCESSION   DACDHL010000294 DACDHL010000000
VERSION     DACDHL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.313"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>420
                     /locus_tag="IV228_003042"
     CDS             <1..>420
                     /locus_tag="IV228_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8718857.1"
                     /translation="QYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNG
                     CREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDV
                     VSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMT"
BASE COUNT          149 a           54 c           76 g          141 t
ORIGIN      
        1 caatatatta agtctaaagt gacgcataat aaaaaaattt cttatcaata ccatgtagga
       61 cagtatttac ccgactggca tccttgggaa gattatcgca atttttttgt ttcaaacaaa
      121 aaaactaatg ggtgtagaga aatattcgct attgaattac cttggattat agatacattt
      181 gggaaagtta aatcgtttac tgtaatgacc cagaaattat ccaatcttga tattgattat
      241 tcggacagtt tcactcttat tattgagcat gaaactggta tagttggaac tttaagtgta
      301 gatgttgttt caagagtagc taaaagaaat ttaactataa ttggtgaaaa gattttaata
      361 gaatgggaag gtaacccgga ttctctattt tcatggaatg aagaaacttc tagtatgaca
//
LOCUS       DACDHL010000295          414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDHL010000295 DACDHL010000000
VERSION     DACDHL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.denovo.314"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(160..>414)
                     /locus_tag="IV228_003043"
     CDS             complement(160..>414)
                     /locus_tag="IV228_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8718858.1"
                     /translation="DQQQLVFFDIDSITGLDRGVQQCQMYMAMTLIWNQALIQGRRQR
                     RLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
BASE COUNT          104 a          100 c           70 g          140 t
ORIGIN      
        1 atttctttta ccttacggta tccttcagcc gacttcaccg agcttcacac aacgttggtc
       61 tgcataaact acttgttgca tgtcggagta tcagtggaag ccctttgtgg cgttacccac
      121 tcatttgact tctcgttata agagttatta caatagtttt tagactactt gcaacccaca
      181 gcttttcact gcagaacagg tcgcaccatt atggcactca tcaatcatcg ccaagaagcg
      241 tttgaagtct tgttcttcca tttgtttttc tttaattaaa cgtcgttgtc ttcttccttg
      301 aatcaatgcc tgattccaaa tcaacgtcat agccatgtac atttgacatt gttgtacccc
      361 tcgatctaag cctgtaatag agtcaatgtc aaaaaagacc aactgttgtt ggtc
//
LOCUS       DACDHL010000296        10087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.guided.090, whole genome shotgun sequence.
ACCESSION   DACDHL010000296 DACDHL010000000
VERSION     DACDHL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.guided.090"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            537..1139
                     /locus_tag="IV228_003044"
     CDS             537..1139
                     /locus_tag="IV228_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718859.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="IV228_003045"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="IV228_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2344..2640
                     /locus_tag="IV228_003046"
     CDS             2344..2640
                     /locus_tag="IV228_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP8718860.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2641..3057
                     /locus_tag="IV228_003047"
     CDS             2641..3057
                     /locus_tag="IV228_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8718861.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="IV228_003048"
     CDS             3077..4621
                     /locus_tag="IV228_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718862.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="IV228_003049"
     CDS             4764..4988
                     /locus_tag="IV228_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8718863.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="IV228_003050"
     CDS             5003..5872
                     /locus_tag="IV228_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8718864.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="IV228_003051"
     CDS             5853..6587
                     /locus_tag="IV228_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8718865.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="IV228_003052"
     CDS             6620..7528
                     /locus_tag="IV228_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8718866.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="IV228_003053"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="IV228_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP8718867.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="IV228_003054"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="IV228_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8718868.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9062..9250)
                     /locus_tag="IV228_003055"
                     /pseudo
     CDS             complement(9062..9250)
                     /locus_tag="IV228_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9230..9802
                     /locus_tag="IV228_003056"
     CDS             9230..9802
                     /locus_tag="IV228_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8718869.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DACDHL010000297         8178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.guided.107, whole genome shotgun sequence.
ACCESSION   DACDHL010000297 DACDHL010000000
VERSION     DACDHL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.guided.107"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..498
                     /locus_tag="IV228_003057"
     CDS             <1..498
                     /locus_tag="IV228_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8718870.1"
                     /translation="FWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEIRETYQ
                     KNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLFTKKML
                     RGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFRAKSKK
                     TSPIK"
     gene            658..2544
                     /locus_tag="IV228_003058"
     CDS             658..2544
                     /locus_tag="IV228_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8718871.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2556..3503
                     /locus_tag="IV228_003059"
     CDS             2556..3503
                     /locus_tag="IV228_003059"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8718872.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3523..4050
                     /locus_tag="IV228_003060"
     CDS             3523..4050
                     /locus_tag="IV228_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8718873.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4113..4766)
                     /locus_tag="IV228_003061"
     CDS             complement(4113..4766)
                     /locus_tag="IV228_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8718874.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4898..5740
                     /locus_tag="IV228_003062"
     CDS             4898..5740
                     /locus_tag="IV228_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8718875.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5898..6794
                     /locus_tag="IV228_003063"
     CDS             5898..6794
                     /locus_tag="IV228_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8718876.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6797..7510
                     /locus_tag="IV228_003064"
     CDS             6797..7510
                     /locus_tag="IV228_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8718877.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            7530..8048
                     /gene="msrA"
                     /locus_tag="IV228_003065"
     CDS             7530..8048
                     /gene="msrA"
                     /locus_tag="IV228_003065"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8718878.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            8045..>8178
                     /locus_tag="IV228_003066"
     CDS             8045..>8178
                     /locus_tag="IV228_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8718879.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     S"
BASE COUNT         2955 a         1422 c         1662 g         2139 t
ORIGIN      
        1 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
       61 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
      121 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
      181 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
      241 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
      301 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
      361 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
      421 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
      481 acgagtccaa taaaataaaa aggtaaaaat tttcaagttt tataaaaaac aaaacatatc
      541 agaacaggat cgaatcggtc ctgttctttt tttgagaaca caaaaagaac aaataccaaa
      601 aaaattttcc tgaagttcac tatttttatg atacaatatc caagataagg agggaacatg
      661 ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac
      721 cagatttctt ttttggtcca cgaaaaagac cgtatcgggt tgattgggac gaacgggacg
      781 ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata gcagcgacgg tgatcggcaa
      841 acagtgtttt atccaactga ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc
      901 gatgaattaa ctgttctcca agcagtcttt caaggaaaca gccccttgat ccaaactgtt
      961 cgagcatatg aggaagcatt gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa
     1021 aaacgttatg ctaaagcaga agaacaaatg aataaagaag atgcttggac tacagatacc
     1081 aacgcaaaga ttattttgca aaaattagga atcagtgaat tagataaaaa aatcagtact
     1141 ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag tgttgattga tgaacccgat
     1201 ttactattac tagacgaacc aacaaaccat ttagattatg aagcgatcga gtggttggaa
     1261 aactatttga aacaataccg tggcgcacta ttgatggtta cccacgatcg ttattttcta
     1321 gatagagtag caaaccgaat cttcgaactt tcatttggaa aattatacga gtataaagga
     1381 aattatgaag cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa
     1441 gaaaagcgca agcggcttta taaacaggaa ttagcttgga tgagggctgg cgtacaggca
     1501 cgtggcacca agcagcaagc acggatcaac cgtttcgaag atctaaagga gaacttgtac
     1561 caagtaaacc aagaagatga tttagagctg aacctagcga cacaacgttt agggaaaaaa
     1621 gtactggaaa tcaaagatgg cagttaccgt ataaatgacc aaacactgct agaacattta
     1681 gatttattga ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa
     1741 tcgacattgc ttaatattct agctggcaga attcccctgg atagcggtac aatgtctatc
     1801 ggtgaaacgg ttcatcttgc gtattataca caagaaaatg aagaaatggc accagataaa
     1861 cggatgattg cttacttaca agaagctgct gaagaagcaa aaacagcaga tggcagtcaa
     1921 atcggagtag cagaattact tgaacgattt ttatttccaa gatttatgca tgggacactg
     1981 ataggcaaac tttcaggcgg agaaaaacgc cggttatttt tgttgaaact gctgattcag
     2041 caacctaatg ttttattatt ggatgaaccg accaatgatt tggacatagc aacattgacg
     2101 attttggaag attattttag aagttttcca ggtgctgtga tcactgtttc ccatgaccgt
     2161 tatttcttgg acaaggtagc agataagtta ttagtattcc aaggaaacgg aaaacaagaa
     2221 ctttattacg gaaacatgtc cagttattta ctgaagcaaa aagagaccca gcagcctgct
     2281 gaaaaagcga aaccaaaaat acagagcaag gaacctgctg aaaagaagaa attatcttac
     2341 atggaacaaa aagaatggga aacgattgaa gatgagattg cagaacttga agaaaaaatc
     2401 tcattgctcc aagaagagat gaatcatcaa ggagataatt tcactcgatt acaagaattg
     2461 caaaacgacg tatctgaaac agaagcacaa cttgaagaaa aaatggcacg gtgggaatac
     2521 ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga acaagcgtat cttgacttag
     2581 gaagaaaatt gttggaagaa gggcatcgta aagaagacag aacaggcaca ggcacattaa
     2641 gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga
     2701 aacgagtacc ttttggattg atcaaaagcg aattgctttg gtttttaaaa ggagatacga
     2761 atatccgcta tctattacaa aataaaaacc atatttggga tgaatgggca tttgaacgct
     2821 acatcaaaag tccagactat aatggaccag atatgactga ttttggccat agatgtttga
     2881 cagatcctga attcaatgaa gaatataaaa agcagagcaa gcttttttgc gagaagatcc
     2941 taaccgatga ttcctttgct gaaacatacg gcgatcttgg acacatctac ggctatcaat
     3001 ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa gtgatcgaag
     3061 caatcaaaaa gactcctgat tctagaagat tgatcgtttc tgcatggaat ccggaagatg
     3121 ttcctagcat ggctttgcct ccttgtcata cgatgtttca attttatgtt caagagggac
     3181 ggctaagctg ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca
     3241 ttgcaagtta tgcgttgctg actcacctaa ttgcacatga aacaggttta gcggttggcg
     3301 aatttgttca cacattagga gatgctcatc tttatatcaa tcatatcgaa caaatgaaag
     3361 aacaattgag ccgagaaata cgcgcatttc cgactttagt tttgaatcag gaaaagcagt
     3421 cagtatttga ttttgagatg gaagatatcc aattagaagg atatgaccca catccggcga
     3481 tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga aaatgttagc tgcaatttgg
     3541 gcacaggatg aaaacggatt gattggaaaa gaagaccgtc ttccttggcg tttgccaaat
     3601 gatctgaaat tcttcaaaca aatgacagaa gcaaatacac tagtgatggg acgaaaaaca
     3661 ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga cgattgtttt gacgagagat
     3721 tcttcctata aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg
     3781 gaaaaagaaa cagacggtat ctttttcatc gctggagggt cagctgttta tcaagaattt
     3841 ctgccatttt gtacgatatt ataccgtaca gtcatccatc atgtattcga tggagatgct
     3901 tattttccac ctgttgactg gtctgattgg tcactgatca atactagtca aggagaaatg
     3961 gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc agcgtaatga gaatcctgta
     4021 aaaagcggga caggcgaatt gacgatataa aaaagctatg gatagactag ctgttattta
     4081 gagctgtccc atccatagct ttattttttt tattaagtaa agaataaaac agagaagtac
     4141 ataaaacctg cacctaacat aacaaacaaa tgccaaacaa catgcatata gcggatagaa
     4201 ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac catcaaaata
     4261 acaccagtca ttccaagaga gtgataaaga ggaacagcag ccacgacaca gagccacccc
     4321 ataaaaatat agatgatcgt agatactttt gatactctct cccgtttatg caacgtcaac
     4381 gctttatata taatgccaag aatcgcaaac aaccagatta atacaaataa agtccaacct
     4441 agccatcctc ggatactcag caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa
     4501 atcgaactat gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat caatgaatga
     4561 aacaaagtag acgttaaaaa taaaagaatc atcatcgatc catagatagc ataagagaca
     4621 atatggaggg aggaatgcaa gtgggcacct tttactaaaa gaaccactag tcccgcgata
     4681 ctcaatccag tcccaattcc atgggtgact gcattaaaca cttcatttaa tatttcatac
     4741 gttcgattaa atttcgtttt ttccatgaaa acggctcctt tactaggtgt aattaatgaa
     4801 aaatcattat ttgtctgtta tactaaaaat agaaattgtc ttacatgtag tttaacatga
     4861 ataaggcatc ttgttaaaga aagagagaga gaaaataatg acaaatgtaa aaatcgtaac
     4921 agattcttca tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc
     4981 gctatccatc atgatcgacg gaacagtata tcccgatgat gaccaactag acggcgaaag
     5041 atttatggaa atgatggcga aagcaaaaaa tctgcccaaa acaagccaac caccaattgg
     5101 agaattcatc tcgatgtatg atgagctagg agaagacggc agtgaagtga tttcgatcca
     5161 tatgacaaaa ggcttgagtg gaacagtaga agcagcaaga caagccagta atctaacaaa
     5221 aacgaaagta acggttatcg acagtgatac cactgaccaa ggattatctt ttcaggtaat
     5281 tcgagcagct gaattagcaa aagaaggtaa aacagtagag aaaatccttg aagaagtcaa
     5341 aaaagtacaa gacaatacaa aattatacat cggaatatcc acactagata acttggtaaa
     5401 aggtggaaga atcagccgaa caactggttt gctgtctagt gttttgaaca tacgcgtggt
     5461 gatgaatttc gcccattctg aattaatccc tgtaactaag gggcgaggaa agaaaacgtt
     5521 cgataaatgg tttaataatt tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat
     5581 cggtatttca catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga
     5641 attattcccg gacatgcata tccctgtact gcatacgaat ccggttatcg ctacgcatac
     5701 tggtaaagac gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag gataagccct
     5761 cagcctgaaa ggcttgcagc ttattctctt tttctttata aaattaggaa aataattgtt
     5821 ttcttttttc ataaagacta cctttttgat aaaatttttg ataacattag aaccaatcga
     5881 atacaagagg tgaaaaaatg aaattaacga aacgacattt gatcctcgtt ttgttcccat
     5941 tattaattgc gatcatcatc tatgcaggtt tagctgcttc tataccgaaa gctggcccac
     6001 taatgaaacc agtaactact gtgagagaag atccaaataa aaaagaaaaa cttcactaca
     6061 tagctatcgg agattcgctg actgaaggag taggagatca aaccaagcaa ggaggattcg
     6121 ttccccttgt cgcaaatgat ctaaaagacc gttataattt gaccgcggtt gaaacagaaa
     6181 actatggtgt aaatggtgaa cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag
     6241 aaattcgaaa caatatcaaa acagctgatt ttatcacact gactgttggc ggtaatgatt
     6301 tgatgaaagt tatccaaaat aatttattcg ggttaacggt caattccttt aaaaagccac
     6361 tgaaaaacta tcaggaaaat gtcacagaac tattggaaga atttcgatcc ctcaataaaa
     6421 ccgcacctat ttacatttta ggcatctaca atccatttta tttgaacttt cctgagatca
     6481 ctgacatgca gacaattgtt aacaattgga acaaaggaac agaaacggtg acaaaaaaaa
     6541 tgccgaactg ctactttatc ccaatcaatg atttgcttta tcaaggattg aatcaggaag
     6601 taggtgtcac agaagaagga acagatcaaa caactagcac gacagaaaat ctaaatggca
     6661 tcaaaaataa tgtgctctat gaagaagatc atttccatcc aaataattta ggttatcaat
     6721 tgatggcaaa tgcagtgaga gatcagctga tcgagacaca aaaccaatgg ttagagaagg
     6781 aggatgatca ataaacatga aaacaagatc tgacgtatct tcaaacaaaa caaagaaaac
     6841 aaattactgg aaattcgcct tccttatcct tcttggggta gtacttggtt cgaccgtatt
     6901 tttaggcagc cgtatttttg ctaatcggga accagaactc ccagaaatac cagctcttac
     6961 ggaaagacaa ggagatcctg tcttaacgat caattcaaat aaagaaaaag tcaatcaaat
     7021 catcagcttt ttccttagtg agtatcaaaa agatagtgat attgaatata aattttattt
     7081 ggaaaatgaa gcactactca atggtacttt cgaagtacta ggttttccta tcgatttcta
     7141 tttatatttt gatccgtatg tgatggaaaa cggaaatgtc caactaaaag ctagaagtct
     7201 ttctatcgga acattgagtt tgccaatcaa agatgtgatg aatatgatca agagaaacta
     7261 taaattacca gattggatcg aagtcaatac agatgatctg actatcatgc ttaggttgga
     7321 tcaattccga atgcaaaacg ggatgtatat taaagcagat aaaatcgact tagtaaatga
     7381 tgacattcgg tttagtctat atttgccaaa agatacctct gatgcttcaa acaactcaaa
     7441 taccaacaaa actagcacca gcagtactga aagcaagaca aaagaaaata aaaaatcatc
     7501 ggatgagtag tattagaggg ggaatcataa tggaacgagc aatatttgca ggaggctgct
     7561 tctggtgtat gatccagcct ttcgacacgc ttcctggtat ccatacagtg atgtcaggat
     7621 atactggagg gcatgtacca gatccgactt atgaacaagt aaaaagccaa acgactggac
     7681 atacagaagc agtggagatt atctatgatc caacattgat ttcatatgaa caattagtag
     7741 aactttactg gcagcaaact gatccaactg acgctttcgg acaattcgag gatcgcggtg
     7801 acaattatcg cccggtgatc ttttatttga cagaagaaca gcggatcatt gctgaaaaaa
     7861 gtaagaaaca gttagctgaa agtggacgtt tcgaacagcc aatcgttaca aaaatcgaac
     7921 cagcccaacc attttatcta gcagaagaag accatcaaga gtattataaa aagcatcctg
     7981 aaaattttaa aagaaaccat gcacgccggg ctgcctttat cgaagaaaat tggaggaacg
     8041 aagggtgaat cgaagctttt ttcattattt aatgacactg cgaggcggtg gcacacatga
     8101 tgctttatct gttttcgcta cagaagccgg taaagacatc caatttccta agcacagctc
     8161 tagctacgaa gaaatttc
//
LOCUS       DACDHL010000298         8166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.guided.108, whole genome shotgun sequence.
ACCESSION   DACDHL010000298 DACDHL010000000
VERSION     DACDHL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.guided.108"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(134..577)
                     /locus_tag="IV228_003067"
     CDS             complement(134..577)
                     /locus_tag="IV228_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8718880.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IV228_003068"
     CDS             complement(795..2135)
                     /locus_tag="IV228_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8718881.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IV228_003069"
     CDS             2404..3300
                     /locus_tag="IV228_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8718882.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IV228_003070"
     CDS             complement(3450..4391)
                     /locus_tag="IV228_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8718883.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IV228_003071"
     CDS             4517..5638
                     /locus_tag="IV228_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8718884.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IV228_003072"
     CDS             5896..7398
                     /locus_tag="IV228_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8718885.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IV228_003073"
     CDS             7465..7992
                     /locus_tag="IV228_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8718886.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2710 a         1515 c         1607 g         2334 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataagagta ttgaaatttt gtttctttag atatatactc
     8161 tagtca
//
LOCUS       DACDHL010000299         5117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.guided.140, whole genome shotgun sequence.
ACCESSION   DACDHL010000299 DACDHL010000000
VERSION     DACDHL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.guided.140"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            245..1213
                     /locus_tag="IV228_003074"
     CDS             245..1213
                     /locus_tag="IV228_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8718887.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IV228_003075"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IV228_003075"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8718888.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IV228_003076"
     CDS             2243..2851
                     /locus_tag="IV228_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8718889.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /gene="vanY"
                     /locus_tag="IV228_003077"
     CDS             3279..4190
                     /gene="vanY"
                     /locus_tag="IV228_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8718890.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ"
                     /locus_tag="IV228_003078"
     CDS             4343..4828
                     /gene="vanZ"
                     /locus_tag="IV228_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP8718891.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT         1627 a          881 c         1140 g         1469 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacta tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aacttta
//
LOCUS       DACDHL010000300         2772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.guided.188, whole genome shotgun sequence.
ACCESSION   DACDHL010000300 DACDHL010000000
VERSION     DACDHL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.guided.188"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            111..194
                     /locus_tag="IV228_003079"
     CDS             111..194
                     /locus_tag="IV228_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8718892.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="IV228_003080"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="IV228_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8718893.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="IV228_003081"
     CDS             complement(1319..2182)
                     /locus_tag="IV228_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8718894.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="IV228_003082"
     CDS             complement(2184..2456)
                     /locus_tag="IV228_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8718895.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="IV228_003083"
     CDS             complement(2473..2682)
                     /locus_tag="IV228_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8718896.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DACDHL010000301         2747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.guided.189, whole genome shotgun sequence.
ACCESSION   DACDHL010000301 DACDHL010000000
VERSION     DACDHL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.guided.189"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            43..642
                     /locus_tag="IV228_003084"
     CDS             43..642
                     /locus_tag="IV228_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8718897.1"
                     /translation="MTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSSVNMQPWRFVV
                     AESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAYESGKMPKEVR
                     DQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGVEADQLAE
                     AFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            996..2474
                     /gene="msr"
                     /locus_tag="IV228_003085"
     CDS             996..2474
                     /gene="msr"
                     /locus_tag="IV228_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8718898.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2521..>2747
                     /locus_tag="IV228_003086"
     CDS             2521..>2747
                     /locus_tag="IV228_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8718899.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPES"
BASE COUNT          938 a          456 c          587 g          766 t
ORIGIN      
        1 aagatttctc tttaccaaca tcatttgaca aagagcccaa ttatgactgg tagaagatcc
       61 gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga aatgatcaaa
      121 gaagcgacga ctgcaccttc ttctgtcaat atgcaaccat ggcgctttgt ggtcgctgaa
      181 agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat
      241 acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa
      301 atctataacc aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta
      361 gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa
      421 gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa
      481 acaaatccaa ttggtggagt cgaagcagat cagttggcag aggcatttgg tttggataaa
      541 gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag
      601 tctgttcgtt tagcaccaga aaagatcact acgttcgaat aaaaggtttt cagttactat
      661 tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa
      721 aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa
      781 aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta
      841 gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag
      901 gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata
      961 attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa
     1021 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
     1081 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
     1141 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
     1201 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
     1261 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
     1321 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
     1381 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
     1441 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
     1501 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
     1561 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
     1621 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
     1681 tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc tctaaacaaa
     1741 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
     1801 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
     1861 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
     1921 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
     1981 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     2041 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     2101 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     2161 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     2221 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     2281 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     2341 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     2401 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     2461 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     2521 ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc
     2581 ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg
     2641 attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg
     2701 ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaa
//
LOCUS       DACDHL010000302         2519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.guided.194, whole genome shotgun sequence.
ACCESSION   DACDHL010000302 DACDHL010000000
VERSION     DACDHL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.guided.194"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..423
                     /locus_tag="IV228_003087"
     CDS             <1..423
                     /locus_tag="IV228_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8718900.1"
                     /translation="PYLIVDLKLCNRTTRITRSTQDLFELIDNIRDKKASLKSLKDTW
                     LDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAG
                     MNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            637..1332
                     /gene="vanR"
                     /locus_tag="IV228_003088"
     CDS             637..1332
                     /gene="vanR"
                     /locus_tag="IV228_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8718901.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1310..2464
                     /locus_tag="IV228_003089"
     CDS             1310..2464
                     /locus_tag="IV228_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8718902.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          867 a          449 c          529 g          674 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ctcgaatcac tcgtagtaca
       61 caagatctat ttgaattaat cgataacata cgagataaaa aggcaagttt aaaatcacta
      121 aaagatacat ggcttgattt atcagaagat aatccataca gccaattctt aattactgta
      181 atggctggtg ttaaccaatt agagcgagat cttattcgga tgagacaacg tgaagggatt
      241 gaattggcta agaaagaagg aaagtttaaa ggtcgattaa agaagtatca taaaaatcac
      301 gcaggaatga attatgcggt aaagctatat aaagaaggaa atatgactgt aaatcaaatt
      361 tgtgaaatta ctaatgtatc tagggcttca ttatacagga aattatcaga agtgaataat
      421 tagccattct gtattccgct aatgggcaat atttttaaag aagaaaagga aactataaaa
      481 tattaacagc ctcctagcga tgccgaaaag ccctttgata aaaaaagaat catcatctta
      541 agaaattctt agtcatttat tatgtaaatg cttataaatt cggccctata atctgataaa
      601 ttattaaggg caaacttatg tgaaagggtg ataactatga gcgataaaat acttattgtg
      661 gatgatgaac atgaaattgc cgatttggtt gaattatact taaaaaacga gaattatacg
      721 gttttcaaat actataccgc caaagaagca ttggaatgta tagacaagtc tgagattgac
      781 cttgccatat tggacatcat gcttcccggc acaagcggcc ttactatctg tcaaaaaata
      841 agggacaagc acacctatcc gattatcatg ctgaccggga aagatacaga ggtagataaa
      901 attacagggt taacaatcgg cgcggatgat tatataacga agccctttcg cccactggag
      961 ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa aattcagtgg agtaaaggag
     1021 cagaacgaaa atgttatcgt ccactccggc cttgtcatta atgttaacac ccatgagtgt
     1081 tatctgaacg agaagcagtt atcccttact cccaccgagt tttcaatact gcgaatcctc
     1141 tgtgaaaaca aggggaatgt ggttagctcc gagctgctat ttcatgagat atggggcgac
     1201 gaatatttca gcaagagcaa caacaccatc accgtgcata tccggcattt gcgcgaaaaa
     1261 atgaacgaca ccattgataa tccgaaatat ataaaaacgg tatggggggt tggttataaa
     1321 attgaaaaat aaaaaaaacg actattccaa actagaacga aaactttaca tgtatatcgt
     1381 tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt cgttcaatga tccgagggaa
     1441 acttggggat tggatcttaa gtattttgga aaacaaatat gacttaaatc acctggacgc
     1501 gatgaaatta tatcaatatt ccatacggaa caatatagat atctttattt atgtggcgat
     1561 tgtcattagt attcttattc tatgtcgcgt catgctttca aaattcgcaa aatactttga
     1621 cgagataaat accggcattg atgtacttat tcagaacgaa gataaacaaa ttgagctttc
     1681 tgcggaaatg gatgttatgg aacaaaagct caacacatta aaacggactc tggaaaagcg
     1741 agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac gttgttatgt acttggcgca
     1801 cgatattaaa acgcccctta catccattat cggttatttg agcctgcttg acgaggctcc
     1861 agacatgccg gtagatcaaa aggcaaagta tgtgcatatc acgttggaca aagcgtatcg
     1921 actcgaacag ctaatcgacg agttttttga gattacacgg tataacctac aaacgataac
     1981 gctaacaaaa acgcacatag acctatacta tatgctggtg cagatgaccg atgaatttta
     2041 tcctcagctt tccgcacatg gaaaacaggc ggttattcac gcccccgagg atctgaccgt
     2101 gtccggcgac cctgataaac tcgcgagagt ctttaacaac attttgaaaa acgccgctgc
     2161 atacagtgag gataacagca tcattgacat taccgcgggc ctctccgggg atgtggtgtc
     2221 aatcgaattc aagaacactg gaagcatccc aaaagataag ctagctgcca tatttgaaaa
     2281 gttctatagg ctggacaatg ctcgttcttc cgatacgggt ggcgcgggac ttggattggc
     2341 gattgcaaaa gaaattattg ttcagcatgg agggcagatt tacgcggaaa gcaatgataa
     2401 ctatacgacg tttagggtag agcttccagc gatgccagac ttggttgata aaaggaggtc
     2461 ctaagagatg tatataattt tggctctttg tcaaatgatg ttggtaaaga gaaatctta
//
LOCUS       DACDHL010000303          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.guided.283, whole genome shotgun sequence.
ACCESSION   DACDHL010000303 DACDHL010000000
VERSION     DACDHL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.guided.283"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..681
                     /gene="aac(6')"
                     /locus_tag="IV228_003090"
     CDS             133..681
                     /gene="aac(6')"
                     /locus_tag="IV228_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8718903.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSK"
BASE COUNT          259 a          107 c          161 g          189 t
ORIGIN      
        1 attttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
       61 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
      121 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
      181 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
      241 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
      301 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
      361 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
      421 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
      481 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
      541 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
      601 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
      661 cctcgaccaa attctaaata ataaaaagag gttgtgacag aagtaatcag cttcaa
//
LOCUS       DACDHL010000304          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62507_2016
            SAMN12640287-rid9892173.guided.284, whole genome shotgun sequence.
ACCESSION   DACDHL010000304 DACDHL010000000
VERSION     DACDHL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640287
            Sequence Read Archive: SRR10040989
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742933.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742933.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:24:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,091
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 36 of 75
            Pseudo Genes (incomplete)         :: 35 of 75
            Pseudo Genes (internal stop)      :: 11 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640287-rid9892173.guided.284"
                     /isolate="E62507_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..681
                     /gene="aac(6')"
                     /locus_tag="IV228_003091"
     CDS             133..681
                     /gene="aac(6')"
                     /locus_tag="IV228_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8718904.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          258 a          108 c          161 g          189 t
ORIGIN      
        1 attttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
       61 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
      121 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
      181 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
      241 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
      301 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
      361 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
      421 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
      481 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
      541 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
      601 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
      661 cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaa
//
